Chc cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Chc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa03614 NM_206729.2
Latest version!
Drosophila melanogaster clathrin heavy chain, transcript variant C (Chc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa03614 NM_001103524.2
Latest version!
Drosophila melanogaster clathrin heavy chain, transcript variant F (Chc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa03614 NM_001298371.1
Latest version!
Drosophila melanogaster clathrin heavy chain, transcript variant G (Chc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa03614 NM_167466.2
Latest version!
Drosophila melanogaster clathrin heavy chain, transcript variant B (Chc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa03614 NM_057694.3
Latest version!
Drosophila melanogaster clathrin heavy chain, transcript variant A (Chc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa03614 NM_001103523.2
Latest version!
Drosophila melanogaster clathrin heavy chain, transcript variant E (Chc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa03614 NM_206728.2
Latest version!
Drosophila melanogaster clathrin heavy chain, transcript variant D (Chc), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa03614
    Clone ID Related Accession (Same CDS sequence) NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
    Accession Version NM_001103524.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product clathrin heavy chain, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001103524.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096994.1
    CDS255..5291
    Translation

    Target ORF information:

    RefSeq Version NM_001103524.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster clathrin heavy chain, transcript variant F (Chc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103524.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03614
    Clone ID Related Accession (Same CDS sequence) NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
    Accession Version NM_057694.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product clathrin heavy chain, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057694.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_477042.1
    CDS374..5410
    Translation

    Target ORF information:

    RefSeq Version NM_057694.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster clathrin heavy chain, transcript variant A (Chc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057694.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03614
    Clone ID Related Accession (Same CDS sequence) NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
    Accession Version NM_206728.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product clathrin heavy chain, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206728.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996451.1
    CDS471..5507
    Translation

    Target ORF information:

    RefSeq Version NM_206728.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster clathrin heavy chain, transcript variant D (Chc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206728.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03614
    Clone ID Related Accession (Same CDS sequence) NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
    Accession Version NM_001103523.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product clathrin heavy chain, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001103523.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096993.1
    CDS361..5397
    Translation

    Target ORF information:

    RefSeq Version NM_001103523.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster clathrin heavy chain, transcript variant E (Chc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103523.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03614
    Clone ID Related Accession (Same CDS sequence) NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
    Accession Version NM_001298371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product clathrin heavy chain, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001285300.1
    CDS466..5502
    Translation

    Target ORF information:

    RefSeq Version NM_001298371.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster clathrin heavy chain, transcript variant G (Chc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001298371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03614
    Clone ID Related Accession (Same CDS sequence) NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
    Accession Version NM_206729.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product clathrin heavy chain, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206729.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996452.1
    CDS412..5448
    Translation

    Target ORF information:

    RefSeq Version NM_206729.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster clathrin heavy chain, transcript variant C (Chc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206729.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03614
    Clone ID Related Accession (Same CDS sequence) NM_001103524.2 , NM_057694.3 , NM_206728.2 , NM_001103523.2 , NM_001298371.1 , NM_206729.2 , NM_167466.2
    Accession Version NM_167466.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product clathrin heavy chain, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167466.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_727901.1
    CDS331..5367
    Translation

    Target ORF information:

    RefSeq Version NM_167466.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster clathrin heavy chain, transcript variant B (Chc), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_167466.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGCAAC CACTGCCCAT CCGCTTTCAG GAGCATTTAC AGCTCACAAA TGTCGGGATC 
    AACGCCAATT CATTTTCATT CAGCACGCTC ACGATGGAAT CGGATAAGTT TATTTGCGTG
    CGGGAGAAGG TGAATGATAC CGCCCAAGTG GTCATCATTG ACATGAACGA TGCCACCAAT
    CCCACGCGGC GTCCCATCTC AGCGGATTCG GCTATCATGA ATCCAGCTAG CAAGGTCATT
    GCGCTCAAAG CGCAAAAGAC GTTGCAGATC TTCAACATTG AGATGAAGTC GAAGATGAAG
    GCGCATACCA TGAACGAGGA TGTGGTGTTC TGGAAGTGGA TCTCCCTCAA TACGCTAGCT
    CTGGTCACAG AAACGAGTGT GTTCCATTGG TCCATGGAGG GCGATTCGAT GCCGCAAAAG
    ATGTTTGACC GGCATTCATC GCTGAACGGT TGTCAAATCA TCAACTACCG CTGCAATGCC
    TCCCAGCAGT GGCTACTCTT GGTCGGCATT TCGGCACTGC CAAGTCGCGT TGCCGGTGCC
    ATGCAGTTGT ATTCGGTGGA GCGTAAGGTG TCCCAGGCGA TCGAGGGGCA TGCGGCCAGT
    TTTGCCACGT TTAAAATCGA TGCCAACAAG GAGCCGACCA CGCTGTTCTG CTTTGCAGTT
    CGTACGGCCA CTGGGGGCAA GCTTCATATT ATCGAAGTTG GTGCTCCGCC GAACGGCAAT
    CAGCCGTTTG CCAAGAAGGC TGTCGATGTC TTCTTTCCGC CGGAAGCACA AAATGATTTC
    CCTGTGGCTA TGCAAGTCTC TGCCAAGTAC GACACCATCT ACTTGATAAC CAAGTATGGA
    TACATACATC TGTATGACAT GGAGACGGCC ACGTGCATAT ACATGAATCG TATATCGGCC
    GATACGATCT TTGTTACCGC ACCGCATGAG GCAAGTGGTG GCATCATTGG CGTCAATCGC
    AAGGGACAGG TCCTCTCCGT GACCGTCGAC GAGGAGCAGA TCATTCCCTA CATCAACACC
    GTTCTGCAGA ATCCCGATTT GGCCCTTCGC ATGGCCGTGC GCAACAATTT GGCTGGTGCC
    GAAGATCTCT TTGTGCGAAA GTTCAACAAG CTCTTTACAG CCGGCCAGTA TGCTGAAGCG
    GCTAAAGTTG CTGCCCTGGC ACCCAAGGCC ATTCTGCGTA CGCCACAGAC GATCCAGCGT
    TTCCAACAGG TGCAGACACC AGCTGGCTCC ACGACTCCGC CGCTGCTGCA ATACTTTGGC
    ATTCTCCTCG ACCAGGGCAA GCTGAACAAG TTCGAGTCTC TCGAGCTGTG CCGTCCCGTC
    TTGCTGCAGG GCAAGAAGCA GCTGTGCGAG AAGTGGCTGA AGGAGGAGAA GTTGGAATGC
    AGCGAGGAGT TGGGTGATCT GGTCAAGGCC TCCGATCTTA CACTTGCCCT GTCCATCTAT
    CTGCGCGCAA ATGTGCCCAA CAAGGTTATC CAATGCTTTG CTGAGACTGG GCAGTTCCAG
    AAGATTGTAC TCTACGCCAA GAAGGTCAAC TATACGCCCG ATTACGTGTT CCTGCTGCGC
    TCCGTGATGC GAAGCAACCC GGAGCAAGGA GCTGGTTTCG CCTCTATGTT GGTGGCCGAG
    GAAGAGCCAC TGGCGGACAT CAATCAGATT GTGGACATCT TCATGGAGCA CTCCATGGTG
    CAGCAGTGCA CTGCATTCCT GCTGGACGCC CTCAAGCATA ACCGTCCCGC CGAGGGTGCC
    CTCCAGACGC GCCTGCTGGA AATGAATCTG ATGTCTGCTC CGCAGGTGGC CGACGCCATC
    CTGGGCAATG CTATGTTCAC CCACTACGAT CGGGCCCACA TTGCCCAGCT GTGCGAGAAG
    GCTGGACTGC TCCAGCGCGC CCTCGAGCAC TACACGGATC TGTATGACAT TAAGCGGGCC
    GTTGTGCACA CGCACATGCT GAATGCCGAA TGGCTGGTCA GTTTCTTTGG CACGCTGTCG
    GTGGAGGACT CGCTGGAATG TCTGAAGGCA ATGCTTACGG CGAATTTGCG CCAGAACTTG
    CAGATCTGTG TGCAGATTGC CACCAAGTAT CACGAACAGC TGACCAACAA GGCACTGATT
    GATCTGTTCG AAGGTTTCAA GAGCTACGAC GGACTGTTCT ACTTCCTGAG CAGCATTGTC
    AACTTCTCAC AGGATCCCGA AGTGCACTTC AAATACATTC AGGCGGCATG CAAGACTAAT
    CAGATTAAGG AGGTGGAGCG AATTTGCCGT GAATCAAACT GCTACAATCC CGAACGGGTG
    AAGAACTTCT TGAAGGAGGC CAAGCTGACG GATCAGCTAC CATTAATTAT TGTTTGTGAT
    CGTTTTGATT TCGTGCACGA CTTGGTGCTT TACCTGTATC GTAACAATCT GCAGAAGTAC
    ATTGAGATCT ATGTGCAGAA AGTGAATCCA TCCCGCTTGC CAGTGGTAGT GGGTGGTCTT
    CTTGATGTTG ATTGCAGTGA GGATATAATT AAAAATCTAA TTCTCGTGGT CAAGGGACAA
    TTCTCAACCG ACGAACTGGT CGAGGAGGTC GAGAAGCGCA ACCGTCTCAA GCTTCTCCTT
    CCCTGGCTGG AGTCCCGAGT TCACGAGGGC TGCGTCGAGC CAGCCACCCA CAACGCGTTG
    GCCAAGATCT ACATTGACTC GAACAACAAT CCCGAGAGAT ATCTTAAGGA GAATCAGTAC
    TACGATAGCC GTGTGGTCGG TCGCTACTGC GAGAAGCGGG ATCCCCATTT GGCGTGTGTC
    GCCTACGAGC GTGGATTGTG CGATCGCGAG CTGATCGCCG TTTGTAACGA GAATTCTCTG
    TTCAAGAGCG AAGCACGCTA CTTGGTTGGT CGCCGCGACG CCGAACTCTG GGCCGAGGTC
    CTTTCGGAGA GCAATCCATA CAAGCGCCAG TTGATCGATC AGGTGGTACA GACCGCTTTG
    TCCGAGACCC AGGATCCCGA TGACATCTCT GTAACGGTCA AAGCATTCAT GACCGCCGAT
    TTGCCCAATG AGCTGATCGA ACTTCTCGAG AAGATTATTC TGGACTCGTC CGTCTTTAGC
    GACCATCGCA ATCTGCAAAA CTTGCTCATT CTCACAGCCA TCAAGGCTGA TCGCACCCGA
    GTCATGGACT ACATTAACCG GCTGGAGAAC TACGATGCAC CGGACATCGC GAACATTGCG
    ATCAGTAATC AGTTGTACGA AGAAGCCTTC GCCATCTTCA AGAAGTTCGA TGTGAACACA
    TCGGCCATTC AGGTGCTCAT CGATCAAGTG AACAACCTGG AGCGGGCTAA CGAGTTCGCC
    GAGCGGTGCA ATGAGCCGGC CGTTTGGTCG CAGCTGGCCA AGGCCCAACT GCAGCAGGGT
    CTGGTCAAGG AGGCTATCGA CTCGTACATC AAGGCTGATG ATCCGAGCGC TTACGTCGAT
    GTCGTCGATG TGGCCAGCAA GGTGGAGTCT TGGGATGACC TCGTTCGCTA TCTGCAAATG
    GCACGCAAGA AGGCGCGCGA ATCTTACATC GAGAGCGAAT TGATCTATGC CTATGCGCGC
    ACTGGACGTC TGGCCGATCT GGAGGAGTTC ATTTCGGGTC CCAACCATGC CGATATCCAG
    AAGATTGGCA ACCGTTGCTT CAGCGACGGC ATGTACGATG CAGCGAAGCT ACTGTACAAC
    AATGTGAGCA ACTTTGCCCG TCTGGCCATC ACTTTGGTCT ACCTGAAGGA GTTCCAAGGG
    GCCGTGGACT CGGCGCGGAA AGCCAACTCA ACGCGCACAT GGAAGGAGGT GTGCTTCGCC
    TGCGTGGACG CCGAGGAGTT TAGGCTAGCT CAGATGTGCG GCCTGCACAT TGTGGTGCAT
    GCCGATGAGC TAGAGGATCT GATTAACTAC TATCAAAACC GTGGATACTT CGATGAATTG
    ATTGCGCTAC TCGAGTCGGC TTTGGGGCTG GAACGTGCGC ACATGGGAAT GTTCACCGAA
    TTAGCTATAC TTTATTCAAA ATTCAAACCT TCCAAAATGC GCGAACACTT GGAGCTGTTC
    TGGTCTCGCG TTAACATTCC AAAAGTTCTG CGTGCCGCTG AATCGGCTCA CTTGTGGTCG
    GAGCTGGTGT TCCTGTACGA TAAGTACGAG GAGTACGATA ACGCCGTCCT GGCCATGATG
    GCTCATCCCA CGGAGGCGTG GCGCGAGGGG CACTTTAAGG ACATTATCAC CAAGGTAGCC
    AACATTGAGC TGTACTACAA GGCTATCGAA TTCTATTTGG ACTTCAAGCC GCTGCTGTTG
    AACGACATGC TGCTCGTGCT GGCACCCAGG ATGGATCACA CTCGTGCTGT TAGTTACTTC
    TCCAAAACCG GCTATTTGCC ACTCGTCAAG CCTTATCTGC GTTCAGTCCA ATCTCTCAAT
    AACAAGGCAA TCAACGAAGC CCTGAACGGA CTCTTAATCG ACGAGGAGGA CTACCAGGGT
    CTGCGCAATT CGATCGATGG ATTTGATAAC TTTGACAACA TTGCGTTGGC ACAGAAACTC
    GAAAAGCACG AACTTACCGA ATTCCGTAGG ATTGCCGCCT ACTTGTACAA GGGAAATAAT
    CGCTGGAAAC AGAGCGTTGA GCTCTGCAAA AAGGATAAAC TCTACAAGGA TGCTATGGAG
    TACGCCGCCG AATCTTGCAA GCAAGATATT GCCGAAGAGT TGTTGGGTTG GTTCCTAGAA
    CGTGACGCTT ACGATTGTTT TGCAGCTTGT CTTTATCAGT GTTACGACTT GCTGCGCCCT
    GATGTTATCT TGGAGTTGGC CTGGAAACAC AAAATCGTTG ACTTTGCCAT GCCCTATTTG
    ATTCAGGTTC TGCGCGAATA CACAACAAAG GTGGACAAAC TGGAGTTGAA CGAGGCTCAG
    CGCGAGAAGG AGGACGATTC CACTGAGCAC AAAAACATTA TTCAGATGGA GCCACAACTG
    ATGATCACCG CTGGCCCAGC AATGGGCATT CCTCCACAAT ATGCACAGAA TTATCCACCT
    GGTGCAGCAA CGGTAACGGC GGCAGGAGGA CGCAACATGG GCTATCCCTA CTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.