CG8974 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8974
Entrez Gene ID	32532
Full Name	CG8974 gene product from transcript CG8974-RD
Synonyms	Dmel\CG8974
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 13E18-13F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_132848.4	NP_573076.1	uncharacterized protein, isoform C
NM_167462.3	NP_727896.1	uncharacterized protein, isoform A
NM_206725.2	NP_996448.1	uncharacterized protein, isoform E
NM_167463.3	NP_727897.1	uncharacterized protein, isoform B
NM_206726.2	NP_996449.1	uncharacterized protein, isoform D

Pathways from BioSystems

Homologies

Homo sapiens (human)	RNF185	NP_689480.2
Caenorhabditis elegans (roundworm)	rnf-5	NP_497830.1
Bos taurus (cattle)	RNF185	NP_001077172.1
Danio rerio (zebrafish)	rnf185	NP_998202.1
Pan troglodytes (chimpanzee)	RNF185	XP_515084.1
Canis lupus familiaris (dog)	RNF185	XP_852634.1
Rattus norvegicus (Norway rat)	Rnf185	NP_001019442.1
Gallus gallus (chicken)	RNF185	NP_001007841.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006041	XP_316088.3
Mus musculus (house mouse)	Rnf185	NP_663330.2
Drosophila melanogaster (fruit fly)	CG8974	NP_727897.1
Drosophila melanogaster (fruit fly)	CG32581	NP_727898.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG8974 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8974 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03609	NM_167462.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa03609	NM_206726.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG8974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa03609	NM_132848.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG8974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa03609	NM_206725.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant E (CG8974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa03609	NM_167463.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8974 ( NM_132848.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573076.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG8974), mRNA.

Price & Availability↑

CloneID	OFa03609
Clone ID Related Accession (Same CDS sequence)	NM_132848.4 , NM_167462.3 , NM_206725.2 , NM_167463.3 , NM_206726.2
Accession Version	NM_132848.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132848.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAC CGAAAAGTGT ACCCACAGCG GAGCTTCCCA GCACATCAAC CGGGGCATCG 
TCGTCCTCAT CGACTTTGTC CAACAACGTG AGCTACGACA AGATAGCCTG GACTCGGGAT 
ATCACAGAAC CCTTGGCTGA GGAGTCATCT ACAAGTCAAT TGCGCCCGCT GGGTACCTCG 
ATTCCAACCA ATTCGACGGC ATCGGAACTG AAGAAGTCCA ACACTTCGTA TTCCTTTACT 
GGAGACTATC TATCTGGTGG CAATAAGGCC GATCTGAAGG GTGGCTATCC ATTCGGCGGC 
ACCGATACGG ACACAAAAGC CAACGAAAAG GACAAGGAGA AGGAACATAC CGCAGACGAC 
TCACTCTACG AGTGCAACAT ATGCCTGGAC ACCGCCAAGG ATGCTGTGGT CAGCATGTGC 
GGCCATCTGT TCTGCTGGCC GTGCCTGCAT CAGTGGCTCC TGACGCGTCC CAATCGCAAA 
CTCTGTCCCG TTTGTAAGGC TGCCGTTGAT AAGGATAAGG TCATACCGCT GTACGGAAGA 
AATAGTACAC ACCAGGAAGA TCCACGTAAC AAAGTTCCAC CACGCCCCGC TGGACAACGT 
ACAGAACCAG ATCCTGTTCC CGGGTTCCCT GGATTCGGAT TCGGCGACGG ATTTCACATG 
TCTTTCGGTA TTGGAGCTTT TCCTTTCGGG TTCATAACAT CTACGCTCAA CTTTGGCGAA 
CCACGTCCAC CTGCTGCTAA TCGGGGAACA AGGCAATACG AGGATGAACA GACCCTGTCC 
AAGCTGTTTT CGTATTTGGC CGTTGTGTGG ATATTGTGGC TGTTCTATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573076.1
CDS	521..1354
Translation	MEEPKSVPTAELPSTSTGASSSSSTLSNNVSYDKIAWTRDITEPLAEESSTSQLRPLGTSIPTNSTASELKKSNTSYSFTGDYLSGGNKADLKGGYPFGGTDTDTKANEKDKEKEHTADDSLYECNICLDTAKDAVVSMCGHLFCWPCLHQWLLTRPNRKLCPVCKAAVDKDKVIPLYGRNSTHQEDPRNKVPPRPAGQRTEPDPVPGFPGFGFGDGFHMSFGIGAFPFGFITSTLNFGEPRPPAANRGTRQYEDEQTLSKLFSYLAVVWILWLFYA

Target ORF information:

RefSeq Version	NM_132848.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG8974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132848.4

ORF Insert Sequence:

ATGGAGGAAC CGAAAAGTGT ACCCACAGCG GAGCTTCCCA GCACATCAAC CGGGGCATCG 
TCGTCCTCAT CGACTTTGTC CAACAACGTG AGCTACGACA AGATAGCCTG GACTCGGGAT 
ATCACAGAAC CCTTGGCTGA GGAGTCATCT ACAAGTCAAT TGCGCCCGCT GGGTACCTCG 
ATTCCAACCA ATTCGACGGC ATCGGAACTG AAGAAGTCCA ACACTTCGTA TTCCTTTACT 
GGAGACTATC TATCTGGTGG CAATAAGGCC GATCTGAAGG GTGGCTATCC ATTCGGCGGC 
ACCGATACGG ACACAAAAGC CAACGAAAAG GACAAGGAGA AGGAACATAC CGCAGACGAC 
TCACTCTACG AGTGCAACAT ATGCCTGGAC ACCGCCAAGG ATGCTGTGGT CAGCATGTGC 
GGCCATCTGT TCTGCTGGCC GTGCCTGCAT CAGTGGCTCC TGACGCGTCC CAATCGCAAA 
CTCTGTCCCG TTTGTAAGGC TGCCGTTGAT AAGGATAAGG TCATACCGCT GTACGGAAGA 
AATAGTACAC ACCAGGAAGA TCCACGTAAC AAAGTTCCAC CACGCCCCGC TGGACAACGT 
ACAGAACCAG ATCCTGTTCC CGGGTTCCCT GGATTCGGAT TCGGCGACGG ATTTCACATG 
TCTTTCGGTA TTGGAGCTTT TCCTTTCGGG TTCATAACAT CTACGCTCAA CTTTGGCGAA 
CCACGTCCAC CTGCTGCTAA TCGGGGAACA AGGCAATACG AGGATGAACA GACCCTGTCC 
AAGCTGTTTT CGTATTTGGC CGTTGTGTGG ATATTGTGGC TGTTCTATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8974 ( NM_167462.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727896.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG8974), mRNA.

Price & Availability↑

CloneID	OFa03609
Clone ID Related Accession (Same CDS sequence)	NM_132848.4 , NM_167462.3 , NM_206725.2 , NM_167463.3 , NM_206726.2
Accession Version	NM_167462.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167462.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAC CGAAAAGTGT ACCCACAGCG GAGCTTCCCA GCACATCAAC CGGGGCATCG 
TCGTCCTCAT CGACTTTGTC CAACAACGTG AGCTACGACA AGATAGCCTG GACTCGGGAT 
ATCACAGAAC CCTTGGCTGA GGAGTCATCT ACAAGTCAAT TGCGCCCGCT GGGTACCTCG 
ATTCCAACCA ATTCGACGGC ATCGGAACTG AAGAAGTCCA ACACTTCGTA TTCCTTTACT 
GGAGACTATC TATCTGGTGG CAATAAGGCC GATCTGAAGG GTGGCTATCC ATTCGGCGGC 
ACCGATACGG ACACAAAAGC CAACGAAAAG GACAAGGAGA AGGAACATAC CGCAGACGAC 
TCACTCTACG AGTGCAACAT ATGCCTGGAC ACCGCCAAGG ATGCTGTGGT CAGCATGTGC 
GGCCATCTGT TCTGCTGGCC GTGCCTGCAT CAGTGGCTCC TGACGCGTCC CAATCGCAAA 
CTCTGTCCCG TTTGTAAGGC TGCCGTTGAT AAGGATAAGG TCATACCGCT GTACGGAAGA 
AATAGTACAC ACCAGGAAGA TCCACGTAAC AAAGTTCCAC CACGCCCCGC TGGACAACGT 
ACAGAACCAG ATCCTGTTCC CGGGTTCCCT GGATTCGGAT TCGGCGACGG ATTTCACATG 
TCTTTCGGTA TTGGAGCTTT TCCTTTCGGG TTCATAACAT CTACGCTCAA CTTTGGCGAA 
CCACGTCCAC CTGCTGCTAA TCGGGGAACA AGGCAATACG AGGATGAACA GACCCTGTCC 
AAGCTGTTTT CGTATTTGGC CGTTGTGTGG ATATTGTGGC TGTTCTATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727896.1
CDS	259..1092
Translation	MEEPKSVPTAELPSTSTGASSSSSTLSNNVSYDKIAWTRDITEPLAEESSTSQLRPLGTSIPTNSTASELKKSNTSYSFTGDYLSGGNKADLKGGYPFGGTDTDTKANEKDKEKEHTADDSLYECNICLDTAKDAVVSMCGHLFCWPCLHQWLLTRPNRKLCPVCKAAVDKDKVIPLYGRNSTHQEDPRNKVPPRPAGQRTEPDPVPGFPGFGFGDGFHMSFGIGAFPFGFITSTLNFGEPRPPAANRGTRQYEDEQTLSKLFSYLAVVWILWLFYA

Target ORF information:

RefSeq Version	NM_167462.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167462.3

ORF Insert Sequence:

ATGGAGGAAC CGAAAAGTGT ACCCACAGCG GAGCTTCCCA GCACATCAAC CGGGGCATCG 
TCGTCCTCAT CGACTTTGTC CAACAACGTG AGCTACGACA AGATAGCCTG GACTCGGGAT 
ATCACAGAAC CCTTGGCTGA GGAGTCATCT ACAAGTCAAT TGCGCCCGCT GGGTACCTCG 
ATTCCAACCA ATTCGACGGC ATCGGAACTG AAGAAGTCCA ACACTTCGTA TTCCTTTACT 
GGAGACTATC TATCTGGTGG CAATAAGGCC GATCTGAAGG GTGGCTATCC ATTCGGCGGC 
ACCGATACGG ACACAAAAGC CAACGAAAAG GACAAGGAGA AGGAACATAC CGCAGACGAC 
TCACTCTACG AGTGCAACAT ATGCCTGGAC ACCGCCAAGG ATGCTGTGGT CAGCATGTGC 
GGCCATCTGT TCTGCTGGCC GTGCCTGCAT CAGTGGCTCC TGACGCGTCC CAATCGCAAA 
CTCTGTCCCG TTTGTAAGGC TGCCGTTGAT AAGGATAAGG TCATACCGCT GTACGGAAGA 
AATAGTACAC ACCAGGAAGA TCCACGTAAC AAAGTTCCAC CACGCCCCGC TGGACAACGT 
ACAGAACCAG ATCCTGTTCC CGGGTTCCCT GGATTCGGAT TCGGCGACGG ATTTCACATG 
TCTTTCGGTA TTGGAGCTTT TCCTTTCGGG TTCATAACAT CTACGCTCAA CTTTGGCGAA 
CCACGTCCAC CTGCTGCTAA TCGGGGAACA AGGCAATACG AGGATGAACA GACCCTGTCC 
AAGCTGTTTT CGTATTTGGC CGTTGTGTGG ATATTGTGGC TGTTCTATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8974 ( NM_206725.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996448.1 Drosophila melanogaster uncharacterized protein, transcript variant E (CG8974), mRNA.

Price & Availability↑

CloneID	OFa03609
Clone ID Related Accession (Same CDS sequence)	NM_132848.4 , NM_167462.3 , NM_206725.2 , NM_167463.3 , NM_206726.2
Accession Version	NM_206725.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206725.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAC CGAAAAGTGT ACCCACAGCG GAGCTTCCCA GCACATCAAC CGGGGCATCG 
TCGTCCTCAT CGACTTTGTC CAACAACGTG AGCTACGACA AGATAGCCTG GACTCGGGAT 
ATCACAGAAC CCTTGGCTGA GGAGTCATCT ACAAGTCAAT TGCGCCCGCT GGGTACCTCG 
ATTCCAACCA ATTCGACGGC ATCGGAACTG AAGAAGTCCA ACACTTCGTA TTCCTTTACT 
GGAGACTATC TATCTGGTGG CAATAAGGCC GATCTGAAGG GTGGCTATCC ATTCGGCGGC 
ACCGATACGG ACACAAAAGC CAACGAAAAG GACAAGGAGA AGGAACATAC CGCAGACGAC 
TCACTCTACG AGTGCAACAT ATGCCTGGAC ACCGCCAAGG ATGCTGTGGT CAGCATGTGC 
GGCCATCTGT TCTGCTGGCC GTGCCTGCAT CAGTGGCTCC TGACGCGTCC CAATCGCAAA 
CTCTGTCCCG TTTGTAAGGC TGCCGTTGAT AAGGATAAGG TCATACCGCT GTACGGAAGA 
AATAGTACAC ACCAGGAAGA TCCACGTAAC AAAGTTCCAC CACGCCCCGC TGGACAACGT 
ACAGAACCAG ATCCTGTTCC CGGGTTCCCT GGATTCGGAT TCGGCGACGG ATTTCACATG 
TCTTTCGGTA TTGGAGCTTT TCCTTTCGGG TTCATAACAT CTACGCTCAA CTTTGGCGAA 
CCACGTCCAC CTGCTGCTAA TCGGGGAACA AGGCAATACG AGGATGAACA GACCCTGTCC 
AAGCTGTTTT CGTATTTGGC CGTTGTGTGG ATATTGTGGC TGTTCTATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996448.1
CDS	104..937
Translation	MEEPKSVPTAELPSTSTGASSSSSTLSNNVSYDKIAWTRDITEPLAEESSTSQLRPLGTSIPTNSTASELKKSNTSYSFTGDYLSGGNKADLKGGYPFGGTDTDTKANEKDKEKEHTADDSLYECNICLDTAKDAVVSMCGHLFCWPCLHQWLLTRPNRKLCPVCKAAVDKDKVIPLYGRNSTHQEDPRNKVPPRPAGQRTEPDPVPGFPGFGFGDGFHMSFGIGAFPFGFITSTLNFGEPRPPAANRGTRQYEDEQTLSKLFSYLAVVWILWLFYA

Target ORF information:

RefSeq Version	NM_206725.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant E (CG8974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206725.2

ORF Insert Sequence:

ATGGAGGAAC CGAAAAGTGT ACCCACAGCG GAGCTTCCCA GCACATCAAC CGGGGCATCG 
TCGTCCTCAT CGACTTTGTC CAACAACGTG AGCTACGACA AGATAGCCTG GACTCGGGAT 
ATCACAGAAC CCTTGGCTGA GGAGTCATCT ACAAGTCAAT TGCGCCCGCT GGGTACCTCG 
ATTCCAACCA ATTCGACGGC ATCGGAACTG AAGAAGTCCA ACACTTCGTA TTCCTTTACT 
GGAGACTATC TATCTGGTGG CAATAAGGCC GATCTGAAGG GTGGCTATCC ATTCGGCGGC 
ACCGATACGG ACACAAAAGC CAACGAAAAG GACAAGGAGA AGGAACATAC CGCAGACGAC 
TCACTCTACG AGTGCAACAT ATGCCTGGAC ACCGCCAAGG ATGCTGTGGT CAGCATGTGC 
GGCCATCTGT TCTGCTGGCC GTGCCTGCAT CAGTGGCTCC TGACGCGTCC CAATCGCAAA 
CTCTGTCCCG TTTGTAAGGC TGCCGTTGAT AAGGATAAGG TCATACCGCT GTACGGAAGA 
AATAGTACAC ACCAGGAAGA TCCACGTAAC AAAGTTCCAC CACGCCCCGC TGGACAACGT 
ACAGAACCAG ATCCTGTTCC CGGGTTCCCT GGATTCGGAT TCGGCGACGG ATTTCACATG 
TCTTTCGGTA TTGGAGCTTT TCCTTTCGGG TTCATAACAT CTACGCTCAA CTTTGGCGAA 
CCACGTCCAC CTGCTGCTAA TCGGGGAACA AGGCAATACG AGGATGAACA GACCCTGTCC 
AAGCTGTTTT CGTATTTGGC CGTTGTGTGG ATATTGTGGC TGTTCTATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8974 ( NM_167463.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727897.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG8974), mRNA.

Price & Availability↑

CloneID	OFa03609
Clone ID Related Accession (Same CDS sequence)	NM_132848.4 , NM_167462.3 , NM_206725.2 , NM_167463.3 , NM_206726.2
Accession Version	NM_167463.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167463.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAC CGAAAAGTGT ACCCACAGCG GAGCTTCCCA GCACATCAAC CGGGGCATCG 
TCGTCCTCAT CGACTTTGTC CAACAACGTG AGCTACGACA AGATAGCCTG GACTCGGGAT 
ATCACAGAAC CCTTGGCTGA GGAGTCATCT ACAAGTCAAT TGCGCCCGCT GGGTACCTCG 
ATTCCAACCA ATTCGACGGC ATCGGAACTG AAGAAGTCCA ACACTTCGTA TTCCTTTACT 
GGAGACTATC TATCTGGTGG CAATAAGGCC GATCTGAAGG GTGGCTATCC ATTCGGCGGC 
ACCGATACGG ACACAAAAGC CAACGAAAAG GACAAGGAGA AGGAACATAC CGCAGACGAC 
TCACTCTACG AGTGCAACAT ATGCCTGGAC ACCGCCAAGG ATGCTGTGGT CAGCATGTGC 
GGCCATCTGT TCTGCTGGCC GTGCCTGCAT CAGTGGCTCC TGACGCGTCC CAATCGCAAA 
CTCTGTCCCG TTTGTAAGGC TGCCGTTGAT AAGGATAAGG TCATACCGCT GTACGGAAGA 
AATAGTACAC ACCAGGAAGA TCCACGTAAC AAAGTTCCAC CACGCCCCGC TGGACAACGT 
ACAGAACCAG ATCCTGTTCC CGGGTTCCCT GGATTCGGAT TCGGCGACGG ATTTCACATG 
TCTTTCGGTA TTGGAGCTTT TCCTTTCGGG TTCATAACAT CTACGCTCAA CTTTGGCGAA 
CCACGTCCAC CTGCTGCTAA TCGGGGAACA AGGCAATACG AGGATGAACA GACCCTGTCC 
AAGCTGTTTT CGTATTTGGC CGTTGTGTGG ATATTGTGGC TGTTCTATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727897.1
CDS	176..1009
Translation	MEEPKSVPTAELPSTSTGASSSSSTLSNNVSYDKIAWTRDITEPLAEESSTSQLRPLGTSIPTNSTASELKKSNTSYSFTGDYLSGGNKADLKGGYPFGGTDTDTKANEKDKEKEHTADDSLYECNICLDTAKDAVVSMCGHLFCWPCLHQWLLTRPNRKLCPVCKAAVDKDKVIPLYGRNSTHQEDPRNKVPPRPAGQRTEPDPVPGFPGFGFGDGFHMSFGIGAFPFGFITSTLNFGEPRPPAANRGTRQYEDEQTLSKLFSYLAVVWILWLFYA

Target ORF information:

RefSeq Version	NM_167463.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167463.3

ORF Insert Sequence:

ATGGAGGAAC CGAAAAGTGT ACCCACAGCG GAGCTTCCCA GCACATCAAC CGGGGCATCG 
TCGTCCTCAT CGACTTTGTC CAACAACGTG AGCTACGACA AGATAGCCTG GACTCGGGAT 
ATCACAGAAC CCTTGGCTGA GGAGTCATCT ACAAGTCAAT TGCGCCCGCT GGGTACCTCG 
ATTCCAACCA ATTCGACGGC ATCGGAACTG AAGAAGTCCA ACACTTCGTA TTCCTTTACT 
GGAGACTATC TATCTGGTGG CAATAAGGCC GATCTGAAGG GTGGCTATCC ATTCGGCGGC 
ACCGATACGG ACACAAAAGC CAACGAAAAG GACAAGGAGA AGGAACATAC CGCAGACGAC 
TCACTCTACG AGTGCAACAT ATGCCTGGAC ACCGCCAAGG ATGCTGTGGT CAGCATGTGC 
GGCCATCTGT TCTGCTGGCC GTGCCTGCAT CAGTGGCTCC TGACGCGTCC CAATCGCAAA 
CTCTGTCCCG TTTGTAAGGC TGCCGTTGAT AAGGATAAGG TCATACCGCT GTACGGAAGA 
AATAGTACAC ACCAGGAAGA TCCACGTAAC AAAGTTCCAC CACGCCCCGC TGGACAACGT 
ACAGAACCAG ATCCTGTTCC CGGGTTCCCT GGATTCGGAT TCGGCGACGG ATTTCACATG 
TCTTTCGGTA TTGGAGCTTT TCCTTTCGGG TTCATAACAT CTACGCTCAA CTTTGGCGAA 
CCACGTCCAC CTGCTGCTAA TCGGGGAACA AGGCAATACG AGGATGAACA GACCCTGTCC 
AAGCTGTTTT CGTATTTGGC CGTTGTGTGG ATATTGTGGC TGTTCTATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8974 ( NM_206726.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996449.1 Drosophila melanogaster uncharacterized protein, transcript variant D (CG8974), mRNA.

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CloneID	OFa03609
Clone ID Related Accession (Same CDS sequence)	NM_132848.4 , NM_167462.3 , NM_206725.2 , NM_167463.3 , NM_206726.2
Accession Version	NM_206726.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206726.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAC CGAAAAGTGT ACCCACAGCG GAGCTTCCCA GCACATCAAC CGGGGCATCG 
TCGTCCTCAT CGACTTTGTC CAACAACGTG AGCTACGACA AGATAGCCTG GACTCGGGAT 
ATCACAGAAC CCTTGGCTGA GGAGTCATCT ACAAGTCAAT TGCGCCCGCT GGGTACCTCG 
ATTCCAACCA ATTCGACGGC ATCGGAACTG AAGAAGTCCA ACACTTCGTA TTCCTTTACT 
GGAGACTATC TATCTGGTGG CAATAAGGCC GATCTGAAGG GTGGCTATCC ATTCGGCGGC 
ACCGATACGG ACACAAAAGC CAACGAAAAG GACAAGGAGA AGGAACATAC CGCAGACGAC 
TCACTCTACG AGTGCAACAT ATGCCTGGAC ACCGCCAAGG ATGCTGTGGT CAGCATGTGC 
GGCCATCTGT TCTGCTGGCC GTGCCTGCAT CAGTGGCTCC TGACGCGTCC CAATCGCAAA 
CTCTGTCCCG TTTGTAAGGC TGCCGTTGAT AAGGATAAGG TCATACCGCT GTACGGAAGA 
AATAGTACAC ACCAGGAAGA TCCACGTAAC AAAGTTCCAC CACGCCCCGC TGGACAACGT 
ACAGAACCAG ATCCTGTTCC CGGGTTCCCT GGATTCGGAT TCGGCGACGG ATTTCACATG 
TCTTTCGGTA TTGGAGCTTT TCCTTTCGGG TTCATAACAT CTACGCTCAA CTTTGGCGAA 
CCACGTCCAC CTGCTGCTAA TCGGGGAACA AGGCAATACG AGGATGAACA GACCCTGTCC 
AAGCTGTTTT CGTATTTGGC CGTTGTGTGG ATATTGTGGC TGTTCTATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996449.1
CDS	67..900
Translation	MEEPKSVPTAELPSTSTGASSSSSTLSNNVSYDKIAWTRDITEPLAEESSTSQLRPLGTSIPTNSTASELKKSNTSYSFTGDYLSGGNKADLKGGYPFGGTDTDTKANEKDKEKEHTADDSLYECNICLDTAKDAVVSMCGHLFCWPCLHQWLLTRPNRKLCPVCKAAVDKDKVIPLYGRNSTHQEDPRNKVPPRPAGQRTEPDPVPGFPGFGFGDGFHMSFGIGAFPFGFITSTLNFGEPRPPAANRGTRQYEDEQTLSKLFSYLAVVWILWLFYA

Target ORF information:

RefSeq Version	NM_206726.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG8974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206726.2

ORF Insert Sequence:

ATGGAGGAAC CGAAAAGTGT ACCCACAGCG GAGCTTCCCA GCACATCAAC CGGGGCATCG 
TCGTCCTCAT CGACTTTGTC CAACAACGTG AGCTACGACA AGATAGCCTG GACTCGGGAT 
ATCACAGAAC CCTTGGCTGA GGAGTCATCT ACAAGTCAAT TGCGCCCGCT GGGTACCTCG 
ATTCCAACCA ATTCGACGGC ATCGGAACTG AAGAAGTCCA ACACTTCGTA TTCCTTTACT 
GGAGACTATC TATCTGGTGG CAATAAGGCC GATCTGAAGG GTGGCTATCC ATTCGGCGGC 
ACCGATACGG ACACAAAAGC CAACGAAAAG GACAAGGAGA AGGAACATAC CGCAGACGAC 
TCACTCTACG AGTGCAACAT ATGCCTGGAC ACCGCCAAGG ATGCTGTGGT CAGCATGTGC 
GGCCATCTGT TCTGCTGGCC GTGCCTGCAT CAGTGGCTCC TGACGCGTCC CAATCGCAAA 
CTCTGTCCCG TTTGTAAGGC TGCCGTTGAT AAGGATAAGG TCATACCGCT GTACGGAAGA 
AATAGTACAC ACCAGGAAGA TCCACGTAAC AAAGTTCCAC CACGCCCCGC TGGACAACGT 
ACAGAACCAG ATCCTGTTCC CGGGTTCCCT GGATTCGGAT TCGGCGACGG ATTTCACATG 
TCTTTCGGTA TTGGAGCTTT TCCTTTCGGG TTCATAACAT CTACGCTCAA CTTTGGCGAA 
CCACGTCCAC CTGCTGCTAA TCGGGGAACA AGGCAATACG AGGATGAACA GACCCTGTCC 
AAGCTGTTTT CGTATTTGGC CGTTGTGTGG ATATTGTGGC TGTTCTATGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8974 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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