Tomosyn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tomosyn
Entrez Gene ID	32217
Full Name	CG17762 gene product from transcript CG17762-RJ
Synonyms	BcDNA:RH19475,CG11056,CG17762,CG2205,Dmel\CG17762,tomosyn
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11B6-11B7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169265.2	NP_001162736.2	tomosyn, isoform K
NM_167329.3	NP_727629.2	tomosyn, isoform C
NM_167330.3	NP_727630.3	tomosyn, isoform L
NM_001258715.2	NP_001245644.1	tomosyn, isoform H
NM_132591.5	NP_572819.4	tomosyn, isoform J
NM_001169264.2	NP_001162735.1	tomosyn, isoform F
NM_167328.4	NP_727628.3	tomosyn, isoform I

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	STXBP5	XP_003311579.1
Homo sapiens (human)	STXBP5	NP_001121187.1
Drosophila melanogaster (fruit fly)	tomosyn	NP_001245644.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000009	XP_003436897.1
Danio rerio (zebrafish)	stxbp5a	NP_001093451.1
Xenopus tropicalis (tropical clawed frog)	stxbp5	XP_002936416.1
	STXBP5	XP_002803916.1
Canis lupus familiaris (dog)	STXBP5	XP_533442.3
Rattus norvegicus (Norway rat)	Stxbp5	NP_848035.1
Bos taurus (cattle)	STXBP5	NP_001178469.1
Mus musculus (house mouse)	Stxbp5	NP_001074813.2
Gallus gallus (chicken)	STXBP5	XP_003641042.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tomosyn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tomosyn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01418	NM_167328.4 Latest version!	Drosophila melanogaster tomosyn, transcript variant I (Tomosyn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa01418	NM_001169264.2 Latest version!	Drosophila melanogaster tomosyn, transcript variant F (Tomosyn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa01418	NM_167329.3 Latest version!	Drosophila melanogaster tomosyn, transcript variant C (Tomosyn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa01419	NM_132591.5 Latest version!	Drosophila melanogaster tomosyn, transcript variant J (Tomosyn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa01419	NM_001258715.2 Latest version!	Drosophila melanogaster tomosyn, transcript variant H (Tomosyn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa21448	NM_001169265.2 Latest version!	Drosophila melanogaster tomosyn, transcript variant K (Tomosyn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa21448	NM_167330.3 Latest version!	Drosophila melanogaster tomosyn, transcript variant L (Tomosyn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tomosyn ( NM_167329.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727629.2 Drosophila melanogaster tomosyn, transcript variant C (Tomosyn), mRNA.

Price & Availability↑

CloneID	OFa01418
Clone ID Related Accession (Same CDS sequence)	NM_167329.3 , NM_001169264.2 , NM_167328.4
Accession Version	NM_167329.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tomosyn, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167329.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGCTGAAC 
GACACGACCA GTCCGGATAG TCCGTCTCTC GAATTTGTAC CGGAGGCTGG CGAAACATCG 
GCCCACGATG GCGTGGCGAC GGCAACTAGC TCACGTCCCA GTTCACCGGA CCCGGTGGTG 
AACAGTGCTC GTGAAACGCT GCTCAGCGGA TCGGCAGCGG CAACGGCGGC TGCGGCAGCA 
TCGGCGGCTG TCATGATGGC CAAGAGTCCC CAGCGGGATG ATGCAGCTCC CAAGGAGCTG 
CAGGACAGAC GAAAGTCCAT GTCCTGGAAA ACGTTCAATT TGAAGCGTCA ATTATCGAAA 
GTCAATATGA AGATCGGCGG CCTGAATGTC AATACCGATA TGGAGTCGCT AAAGAATAGC 
TCCATTTTCT ACACGCCCAA GGAGGTGTCA CCGGAGGCTG CTACGCCACC GGCAACAGAA 
GAGGAGGCAA CGCCGGAGGG CAAGGAGGAC GAGTCCCTGG ATGATGGCGA AATGGCGACC 
AACGCGGGAG CAGCAGCAGC TGCATCACCA TCGCCGGCAG TTCGTGGCAA ATTTCGCTCA 
TCCACCACGG ATTCACCTGA TGAGGGCGCC AGCAGTAGCA TTGCCAGCGG CATTCGTCGT 
GTGGACTTTG AACAGCCGGC TGGGGTCGAG GTGGAGCGAC CAAACAACCT GCCACTGGCG 
GATGCACCAG CTCCACTGAA GCCCGCCCGT CAAAAGTTCA AGGAAAAGTC GCGCGATCAG 
CGACTTTTAT CCGTTCCGAA CATTAAGTAT CAGCCGCGAG ATTCGCGTGG CGGTGCTCGG 
AACCTCAAGA ATGATGTCTC ACCGCTGATG GGCGGTGGTG GCGGTGGAGG AGGCGTTGTT 
GGTGCCAGCA GCGGCATTGG TGGAAGCGTC GCTGGAGGCA AGAAGATACA TAAATTGGAT 
GGAACTTTCT CAAGATCCAG ATCATCGTCA ATGTCCAGCA TTGATATGTC TTCCTCTGAA 
TCTGTTACCT GTTTAGCTTT TATCGAGAGT TATGCAAAAC GAAATGATAT TTTGACACTT 
GTTCCTACTT TGTGGATTGG AACTAGTTTC GGTTCGATAC TGACATTGTT CATCACCATG 
CCCGAGCGAG ATGTGCGCAA ATCTCAGCCA GTATTGATAA CAATAAATGG TGGCCCCGTG 
GTACGACTCA AAGGATCCAT AACTTCCATG TCTTTTTTAG ACTCATACGG TTCGATCATA 
CCCTATACGT TTGAGACCTG GCGTGACGAG AAGAGGGACA ACAAAGATCG CACACCAACA 
AAGAGCAGCA GCCGCACCAG TCCCACATTC ACACCGACAA CTGCCAATAC ATTATCAAAC 
ACTTCGGTCG CGGGTGGTGC GTCCGCAGGC GGAGGAGCTG GCGGCGGTGG TGGTGCCGGC 
GGTGGTGCGG CCAGCGGTGG CGCAGCCGCA GGAGGGCCAG CCGGCGGTGG TGCCGGAGCA 
GGATCGGCGG CTGGAGCGGC CGGCGGCGTT GGAAGCGGTG TAACCAGCGG CGGGGGGATG 
AGCAGCAGCA GCGCCAGCAG CAGCGGCATC AGCGGAAGTG TGAGCACCGG CCTGGAAACT 
CTCACCGATC GACAATACAT CGTTATCGCA AGCGAGAAGC AAACAAAAGT CTTCGATGTC 
GCCAACCAGT GCTGCATCAA CCGCATACAA TTATCGGAAA TGGATTTTGC AGTCAAGGCA 
GAAACTATCA CGATGAAAGA TGGCTCTTGC TTAGCAACCT ATCTTTCGAA TGGCCATCTA 
ATGGTACATA GTCTTCCTAG TCTAAAACTG TTATTGGATA CTGATTTCTT ACCACTTATG 
GAACTAAGTT TTCAAACAAA ATGTAAACAG GGAATTGTGG ATCCAATGCT TTCGATATGG 
GGTCAACAGA TCATTGTTCA TGAAGATACA ACTCAAATAT CAAAAATATT TTGCTTTAGT 
CATAAAGGTC ATGGATTGTA CATGGCATCG CCCACCGAAA TTCAAAAATT CACGATATCA 
TCGGAATTTT GTCAATTTAT TTTGGAGATG ATGGGTGAAC TATATACGGT ACACGAAATG 
CCCGAACAGC CGAAGGAGGG TTTCTTCAAG GGTCTATTTG GTGGTGGCGC CAAACTCCTG 
GATCGCGAGG AGCTATTTGG CGAGCAGAGC GGGAAACCGA ATCGATCGGT GGCACGGCAC 
ATACCCGGCC CCAATTTGGA GCAGCTCGGC CAGAGGGCTT CCACCGCCGC ATCGGAGATC 
AGCCGGGCGC ACCAGCTGGC CATGGAGCGC GGCGAGAAGC TAAATTTGCT GGAGGAGCGC 
GCCGAGCGCA TGGCCAACAC TGCTCAAGAT TTCAGCGGCA CTGCGCATCA ATTAATGCTT 
AAATATAAAG ACAAGAAGTG GTATCAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727629.2
CDS	839..5251
Translation	MKKFTFKGVLDGFRQSVQPQATRQEQEIQEQLKADHFTLKKTFRHGFPYSPTSFAFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTVTADDTIHLWSIRQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYIINWNKAIEVVRTSHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWHYEGKYFVSSHTDGSICSWPTKPQAKPQSQVCPHAKINKDGNAEKCKPIYKVDLKSSATGETFTIFSGGMPSEKGSKSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLIDLLTPGFPCFESPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGEWSPASCSYSEIVITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPESDNPLAVHLIFLCSESRRLCVAGAMGQVMLFKFRKVESTSEVLVLEIPILYENFDDIYGTSPECDFLTGHQVQKAESSDSDKTAECSLKVRNGAQRKPPGFQSQLVCLTTGPNRRNVQVTSLCISSSYGLMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKRIENKEEQSRSPSSDQLNDTTSPDSPSLEFVPEAGETSAHDGVATATSSRPSSPDPVVNSARETLLSGSAAATAAAAASAAVMMAKSPQRDDAAPKELQDRRKSMSWKTFNLKRQLSKVNMKIGGLNVNTDMESLKNSSIFYTPKEVSPEAATPPATEEEATPEGKEDESLDDGEMATNAGAAAAASPSPAVRGKFRSSTTDSPDEGASSSIASGIRRVDFEQPAGVEVERPNNLPLADAPAPLKPARQKFKEKSRDQRLLSVPNIKYQPRDSRGGARNLKNDVSPLMGGGGGGGGVVGASSGIGGSVAGGKKIHKLDGTFSRSRSSSMSSIDMSSSESVTCLAFIESYAKRNDILTLVPTLWIGTSFGSILTLFITMPERDVRKSQPVLITINGGPVVRLKGSITSMSFLDSYGSIIPYTFETWRDEKRDNKDRTPTKSSSRTSPTFTPTTANTLSNTSVAGGASAGGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGGMSSSSASSSGISGSVSTGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETITMKDGSCLATYLSNGHLMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHEDTTQISKIFCFSHKGHGLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFGGGAKLLDREELFGEQSGKPNRSVARHIPGPNLEQLGQRASTAASEISRAHQLAMERGEKLNLLEERAERMANTAQDFSGTAHQLMLKYKDKKWYQL

Target ORF information:

RefSeq Version	NM_167329.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tomosyn, transcript variant C (Tomosyn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167329.3

ORF Insert Sequence:

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGCTGAAC 
GACACGACCA GTCCGGATAG TCCGTCTCTC GAATTTGTAC CGGAGGCTGG CGAAACATCG 
GCCCACGATG GCGTGGCGAC GGCAACTAGC TCACGTCCCA GTTCACCGGA CCCGGTGGTG 
AACAGTGCTC GTGAAACGCT GCTCAGCGGA TCGGCAGCGG CAACGGCGGC TGCGGCAGCA 
TCGGCGGCTG TCATGATGGC CAAGAGTCCC CAGCGGGATG ATGCAGCTCC CAAGGAGCTG 
CAGGACAGAC GAAAGTCCAT GTCCTGGAAA ACGTTCAATT TGAAGCGTCA ATTATCGAAA 
GTCAATATGA AGATCGGCGG CCTGAATGTC AATACCGATA TGGAGTCGCT AAAGAATAGC 
TCCATTTTCT ACACGCCCAA GGAGGTGTCA CCGGAGGCTG CTACGCCACC GGCAACAGAA 
GAGGAGGCAA CGCCGGAGGG CAAGGAGGAC GAGTCCCTGG ATGATGGCGA AATGGCGACC 
AACGCGGGAG CAGCAGCAGC TGCATCACCA TCGCCGGCAG TTCGTGGCAA ATTTCGCTCA 
TCCACCACGG ATTCACCTGA TGAGGGCGCC AGCAGTAGCA TTGCCAGCGG CATTCGTCGT 
GTGGACTTTG AACAGCCGGC TGGGGTCGAG GTGGAGCGAC CAAACAACCT GCCACTGGCG 
GATGCACCAG CTCCACTGAA GCCCGCCCGT CAAAAGTTCA AGGAAAAGTC GCGCGATCAG 
CGACTTTTAT CCGTTCCGAA CATTAAGTAT CAGCCGCGAG ATTCGCGTGG CGGTGCTCGG 
AACCTCAAGA ATGATGTCTC ACCGCTGATG GGCGGTGGTG GCGGTGGAGG AGGCGTTGTT 
GGTGCCAGCA GCGGCATTGG TGGAAGCGTC GCTGGAGGCA AGAAGATACA TAAATTGGAT 
GGAACTTTCT CAAGATCCAG ATCATCGTCA ATGTCCAGCA TTGATATGTC TTCCTCTGAA 
TCTGTTACCT GTTTAGCTTT TATCGAGAGT TATGCAAAAC GAAATGATAT TTTGACACTT 
GTTCCTACTT TGTGGATTGG AACTAGTTTC GGTTCGATAC TGACATTGTT CATCACCATG 
CCCGAGCGAG ATGTGCGCAA ATCTCAGCCA GTATTGATAA CAATAAATGG TGGCCCCGTG 
GTACGACTCA AAGGATCCAT AACTTCCATG TCTTTTTTAG ACTCATACGG TTCGATCATA 
CCCTATACGT TTGAGACCTG GCGTGACGAG AAGAGGGACA ACAAAGATCG CACACCAACA 
AAGAGCAGCA GCCGCACCAG TCCCACATTC ACACCGACAA CTGCCAATAC ATTATCAAAC 
ACTTCGGTCG CGGGTGGTGC GTCCGCAGGC GGAGGAGCTG GCGGCGGTGG TGGTGCCGGC 
GGTGGTGCGG CCAGCGGTGG CGCAGCCGCA GGAGGGCCAG CCGGCGGTGG TGCCGGAGCA 
GGATCGGCGG CTGGAGCGGC CGGCGGCGTT GGAAGCGGTG TAACCAGCGG CGGGGGGATG 
AGCAGCAGCA GCGCCAGCAG CAGCGGCATC AGCGGAAGTG TGAGCACCGG CCTGGAAACT 
CTCACCGATC GACAATACAT CGTTATCGCA AGCGAGAAGC AAACAAAAGT CTTCGATGTC 
GCCAACCAGT GCTGCATCAA CCGCATACAA TTATCGGAAA TGGATTTTGC AGTCAAGGCA 
GAAACTATCA CGATGAAAGA TGGCTCTTGC TTAGCAACCT ATCTTTCGAA TGGCCATCTA 
ATGGTACATA GTCTTCCTAG TCTAAAACTG TTATTGGATA CTGATTTCTT ACCACTTATG 
GAACTAAGTT TTCAAACAAA ATGTAAACAG GGAATTGTGG ATCCAATGCT TTCGATATGG 
GGTCAACAGA TCATTGTTCA TGAAGATACA ACTCAAATAT CAAAAATATT TTGCTTTAGT 
CATAAAGGTC ATGGATTGTA CATGGCATCG CCCACCGAAA TTCAAAAATT CACGATATCA 
TCGGAATTTT GTCAATTTAT TTTGGAGATG ATGGGTGAAC TATATACGGT ACACGAAATG 
CCCGAACAGC CGAAGGAGGG TTTCTTCAAG GGTCTATTTG GTGGTGGCGC CAAACTCCTG 
GATCGCGAGG AGCTATTTGG CGAGCAGAGC GGGAAACCGA ATCGATCGGT GGCACGGCAC 
ATACCCGGCC CCAATTTGGA GCAGCTCGGC CAGAGGGCTT CCACCGCCGC ATCGGAGATC 
AGCCGGGCGC ACCAGCTGGC CATGGAGCGC GGCGAGAAGC TAAATTTGCT GGAGGAGCGC 
GCCGAGCGCA TGGCCAACAC TGCTCAAGAT TTCAGCGGCA CTGCGCATCA ATTAATGCTT 
AAATATAAAG ACAAGAAGTG GTATCAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tomosyn ( NM_001169264.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162735.1 Drosophila melanogaster tomosyn, transcript variant F (Tomosyn), mRNA.

Price & Availability↑

CloneID	OFa01418
Clone ID Related Accession (Same CDS sequence)	NM_167329.3 , NM_001169264.2 , NM_167328.4
Accession Version	NM_001169264.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tomosyn, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001169264.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGCTGAAC 
GACACGACCA GTCCGGATAG TCCGTCTCTC GAATTTGTAC CGGAGGCTGG CGAAACATCG 
GCCCACGATG GCGTGGCGAC GGCAACTAGC TCACGTCCCA GTTCACCGGA CCCGGTGGTG 
AACAGTGCTC GTGAAACGCT GCTCAGCGGA TCGGCAGCGG CAACGGCGGC TGCGGCAGCA 
TCGGCGGCTG TCATGATGGC CAAGAGTCCC CAGCGGGATG ATGCAGCTCC CAAGGAGCTG 
CAGGACAGAC GAAAGTCCAT GTCCTGGAAA ACGTTCAATT TGAAGCGTCA ATTATCGAAA 
GTCAATATGA AGATCGGCGG CCTGAATGTC AATACCGATA TGGAGTCGCT AAAGAATAGC 
TCCATTTTCT ACACGCCCAA GGAGGTGTCA CCGGAGGCTG CTACGCCACC GGCAACAGAA 
GAGGAGGCAA CGCCGGAGGG CAAGGAGGAC GAGTCCCTGG ATGATGGCGA AATGGCGACC 
AACGCGGGAG CAGCAGCAGC TGCATCACCA TCGCCGGCAG TTCGTGGCAA ATTTCGCTCA 
TCCACCACGG ATTCACCTGA TGAGGGCGCC AGCAGTAGCA TTGCCAGCGG CATTCGTCGT 
GTGGACTTTG AACAGCCGGC TGGGGTCGAG GTGGAGCGAC CAAACAACCT GCCACTGGCG 
GATGCACCAG CTCCACTGAA GCCCGCCCGT CAAAAGTTCA AGGAAAAGTC GCGCGATCAG 
CGACTTTTAT CCGTTCCGAA CATTAAGTAT CAGCCGCGAG ATTCGCGTGG CGGTGCTCGG 
AACCTCAAGA ATGATGTCTC ACCGCTGATG GGCGGTGGTG GCGGTGGAGG AGGCGTTGTT 
GGTGCCAGCA GCGGCATTGG TGGAAGCGTC GCTGGAGGCA AGAAGATACA TAAATTGGAT 
GGAACTTTCT CAAGATCCAG ATCATCGTCA ATGTCCAGCA TTGATATGTC TTCCTCTGAA 
TCTGTTACCT GTTTAGCTTT TATCGAGAGT TATGCAAAAC GAAATGATAT TTTGACACTT 
GTTCCTACTT TGTGGATTGG AACTAGTTTC GGTTCGATAC TGACATTGTT CATCACCATG 
CCCGAGCGAG ATGTGCGCAA ATCTCAGCCA GTATTGATAA CAATAAATGG TGGCCCCGTG 
GTACGACTCA AAGGATCCAT AACTTCCATG TCTTTTTTAG ACTCATACGG TTCGATCATA 
CCCTATACGT TTGAGACCTG GCGTGACGAG AAGAGGGACA ACAAAGATCG CACACCAACA 
AAGAGCAGCA GCCGCACCAG TCCCACATTC ACACCGACAA CTGCCAATAC ATTATCAAAC 
ACTTCGGTCG CGGGTGGTGC GTCCGCAGGC GGAGGAGCTG GCGGCGGTGG TGGTGCCGGC 
GGTGGTGCGG CCAGCGGTGG CGCAGCCGCA GGAGGGCCAG CCGGCGGTGG TGCCGGAGCA 
GGATCGGCGG CTGGAGCGGC CGGCGGCGTT GGAAGCGGTG TAACCAGCGG CGGGGGGATG 
AGCAGCAGCA GCGCCAGCAG CAGCGGCATC AGCGGAAGTG TGAGCACCGG CCTGGAAACT 
CTCACCGATC GACAATACAT CGTTATCGCA AGCGAGAAGC AAACAAAAGT CTTCGATGTC 
GCCAACCAGT GCTGCATCAA CCGCATACAA TTATCGGAAA TGGATTTTGC AGTCAAGGCA 
GAAACTATCA CGATGAAAGA TGGCTCTTGC TTAGCAACCT ATCTTTCGAA TGGCCATCTA 
ATGGTACATA GTCTTCCTAG TCTAAAACTG TTATTGGATA CTGATTTCTT ACCACTTATG 
GAACTAAGTT TTCAAACAAA ATGTAAACAG GGAATTGTGG ATCCAATGCT TTCGATATGG 
GGTCAACAGA TCATTGTTCA TGAAGATACA ACTCAAATAT CAAAAATATT TTGCTTTAGT 
CATAAAGGTC ATGGATTGTA CATGGCATCG CCCACCGAAA TTCAAAAATT CACGATATCA 
TCGGAATTTT GTCAATTTAT TTTGGAGATG ATGGGTGAAC TATATACGGT ACACGAAATG 
CCCGAACAGC CGAAGGAGGG TTTCTTCAAG GGTCTATTTG GTGGTGGCGC CAAACTCCTG 
GATCGCGAGG AGCTATTTGG CGAGCAGAGC GGGAAACCGA ATCGATCGGT GGCACGGCAC 
ATACCCGGCC CCAATTTGGA GCAGCTCGGC CAGAGGGCTT CCACCGCCGC ATCGGAGATC 
AGCCGGGCGC ACCAGCTGGC CATGGAGCGC GGCGAGAAGC TAAATTTGCT GGAGGAGCGC 
GCCGAGCGCA TGGCCAACAC TGCTCAAGAT TTCAGCGGCA CTGCGCATCA ATTAATGCTT 
AAATATAAAG ACAAGAAGTG GTATCAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162735.1
CDS	639..5051
Translation	MKKFTFKGVLDGFRQSVQPQATRQEQEIQEQLKADHFTLKKTFRHGFPYSPTSFAFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTVTADDTIHLWSIRQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYIINWNKAIEVVRTSHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWHYEGKYFVSSHTDGSICSWPTKPQAKPQSQVCPHAKINKDGNAEKCKPIYKVDLKSSATGETFTIFSGGMPSEKGSKSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLIDLLTPGFPCFESPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGEWSPASCSYSEIVITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPESDNPLAVHLIFLCSESRRLCVAGAMGQVMLFKFRKVESTSEVLVLEIPILYENFDDIYGTSPECDFLTGHQVQKAESSDSDKTAECSLKVRNGAQRKPPGFQSQLVCLTTGPNRRNVQVTSLCISSSYGLMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKRIENKEEQSRSPSSDQLNDTTSPDSPSLEFVPEAGETSAHDGVATATSSRPSSPDPVVNSARETLLSGSAAATAAAAASAAVMMAKSPQRDDAAPKELQDRRKSMSWKTFNLKRQLSKVNMKIGGLNVNTDMESLKNSSIFYTPKEVSPEAATPPATEEEATPEGKEDESLDDGEMATNAGAAAAASPSPAVRGKFRSSTTDSPDEGASSSIASGIRRVDFEQPAGVEVERPNNLPLADAPAPLKPARQKFKEKSRDQRLLSVPNIKYQPRDSRGGARNLKNDVSPLMGGGGGGGGVVGASSGIGGSVAGGKKIHKLDGTFSRSRSSSMSSIDMSSSESVTCLAFIESYAKRNDILTLVPTLWIGTSFGSILTLFITMPERDVRKSQPVLITINGGPVVRLKGSITSMSFLDSYGSIIPYTFETWRDEKRDNKDRTPTKSSSRTSPTFTPTTANTLSNTSVAGGASAGGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGGMSSSSASSSGISGSVSTGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETITMKDGSCLATYLSNGHLMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHEDTTQISKIFCFSHKGHGLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFGGGAKLLDREELFGEQSGKPNRSVARHIPGPNLEQLGQRASTAASEISRAHQLAMERGEKLNLLEERAERMANTAQDFSGTAHQLMLKYKDKKWYQL

Target ORF information:

RefSeq Version	NM_001169264.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tomosyn, transcript variant F (Tomosyn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169264.2

ORF Insert Sequence:

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGCTGAAC 
GACACGACCA GTCCGGATAG TCCGTCTCTC GAATTTGTAC CGGAGGCTGG CGAAACATCG 
GCCCACGATG GCGTGGCGAC GGCAACTAGC TCACGTCCCA GTTCACCGGA CCCGGTGGTG 
AACAGTGCTC GTGAAACGCT GCTCAGCGGA TCGGCAGCGG CAACGGCGGC TGCGGCAGCA 
TCGGCGGCTG TCATGATGGC CAAGAGTCCC CAGCGGGATG ATGCAGCTCC CAAGGAGCTG 
CAGGACAGAC GAAAGTCCAT GTCCTGGAAA ACGTTCAATT TGAAGCGTCA ATTATCGAAA 
GTCAATATGA AGATCGGCGG CCTGAATGTC AATACCGATA TGGAGTCGCT AAAGAATAGC 
TCCATTTTCT ACACGCCCAA GGAGGTGTCA CCGGAGGCTG CTACGCCACC GGCAACAGAA 
GAGGAGGCAA CGCCGGAGGG CAAGGAGGAC GAGTCCCTGG ATGATGGCGA AATGGCGACC 
AACGCGGGAG CAGCAGCAGC TGCATCACCA TCGCCGGCAG TTCGTGGCAA ATTTCGCTCA 
TCCACCACGG ATTCACCTGA TGAGGGCGCC AGCAGTAGCA TTGCCAGCGG CATTCGTCGT 
GTGGACTTTG AACAGCCGGC TGGGGTCGAG GTGGAGCGAC CAAACAACCT GCCACTGGCG 
GATGCACCAG CTCCACTGAA GCCCGCCCGT CAAAAGTTCA AGGAAAAGTC GCGCGATCAG 
CGACTTTTAT CCGTTCCGAA CATTAAGTAT CAGCCGCGAG ATTCGCGTGG CGGTGCTCGG 
AACCTCAAGA ATGATGTCTC ACCGCTGATG GGCGGTGGTG GCGGTGGAGG AGGCGTTGTT 
GGTGCCAGCA GCGGCATTGG TGGAAGCGTC GCTGGAGGCA AGAAGATACA TAAATTGGAT 
GGAACTTTCT CAAGATCCAG ATCATCGTCA ATGTCCAGCA TTGATATGTC TTCCTCTGAA 
TCTGTTACCT GTTTAGCTTT TATCGAGAGT TATGCAAAAC GAAATGATAT TTTGACACTT 
GTTCCTACTT TGTGGATTGG AACTAGTTTC GGTTCGATAC TGACATTGTT CATCACCATG 
CCCGAGCGAG ATGTGCGCAA ATCTCAGCCA GTATTGATAA CAATAAATGG TGGCCCCGTG 
GTACGACTCA AAGGATCCAT AACTTCCATG TCTTTTTTAG ACTCATACGG TTCGATCATA 
CCCTATACGT TTGAGACCTG GCGTGACGAG AAGAGGGACA ACAAAGATCG CACACCAACA 
AAGAGCAGCA GCCGCACCAG TCCCACATTC ACACCGACAA CTGCCAATAC ATTATCAAAC 
ACTTCGGTCG CGGGTGGTGC GTCCGCAGGC GGAGGAGCTG GCGGCGGTGG TGGTGCCGGC 
GGTGGTGCGG CCAGCGGTGG CGCAGCCGCA GGAGGGCCAG CCGGCGGTGG TGCCGGAGCA 
GGATCGGCGG CTGGAGCGGC CGGCGGCGTT GGAAGCGGTG TAACCAGCGG CGGGGGGATG 
AGCAGCAGCA GCGCCAGCAG CAGCGGCATC AGCGGAAGTG TGAGCACCGG CCTGGAAACT 
CTCACCGATC GACAATACAT CGTTATCGCA AGCGAGAAGC AAACAAAAGT CTTCGATGTC 
GCCAACCAGT GCTGCATCAA CCGCATACAA TTATCGGAAA TGGATTTTGC AGTCAAGGCA 
GAAACTATCA CGATGAAAGA TGGCTCTTGC TTAGCAACCT ATCTTTCGAA TGGCCATCTA 
ATGGTACATA GTCTTCCTAG TCTAAAACTG TTATTGGATA CTGATTTCTT ACCACTTATG 
GAACTAAGTT TTCAAACAAA ATGTAAACAG GGAATTGTGG ATCCAATGCT TTCGATATGG 
GGTCAACAGA TCATTGTTCA TGAAGATACA ACTCAAATAT CAAAAATATT TTGCTTTAGT 
CATAAAGGTC ATGGATTGTA CATGGCATCG CCCACCGAAA TTCAAAAATT CACGATATCA 
TCGGAATTTT GTCAATTTAT TTTGGAGATG ATGGGTGAAC TATATACGGT ACACGAAATG 
CCCGAACAGC CGAAGGAGGG TTTCTTCAAG GGTCTATTTG GTGGTGGCGC CAAACTCCTG 
GATCGCGAGG AGCTATTTGG CGAGCAGAGC GGGAAACCGA ATCGATCGGT GGCACGGCAC 
ATACCCGGCC CCAATTTGGA GCAGCTCGGC CAGAGGGCTT CCACCGCCGC ATCGGAGATC 
AGCCGGGCGC ACCAGCTGGC CATGGAGCGC GGCGAGAAGC TAAATTTGCT GGAGGAGCGC 
GCCGAGCGCA TGGCCAACAC TGCTCAAGAT TTCAGCGGCA CTGCGCATCA ATTAATGCTT 
AAATATAAAG ACAAGAAGTG GTATCAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tomosyn ( NM_167328.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727628.3 Drosophila melanogaster tomosyn, transcript variant I (Tomosyn), mRNA.

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CloneID	OFa01418
Clone ID Related Accession (Same CDS sequence)	NM_167329.3 , NM_001169264.2 , NM_167328.4
Accession Version	NM_167328.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tomosyn, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167328.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGCTGAAC 
GACACGACCA GTCCGGATAG TCCGTCTCTC GAATTTGTAC CGGAGGCTGG CGAAACATCG 
GCCCACGATG GCGTGGCGAC GGCAACTAGC TCACGTCCCA GTTCACCGGA CCCGGTGGTG 
AACAGTGCTC GTGAAACGCT GCTCAGCGGA TCGGCAGCGG CAACGGCGGC TGCGGCAGCA 
TCGGCGGCTG TCATGATGGC CAAGAGTCCC CAGCGGGATG ATGCAGCTCC CAAGGAGCTG 
CAGGACAGAC GAAAGTCCAT GTCCTGGAAA ACGTTCAATT TGAAGCGTCA ATTATCGAAA 
GTCAATATGA AGATCGGCGG CCTGAATGTC AATACCGATA TGGAGTCGCT AAAGAATAGC 
TCCATTTTCT ACACGCCCAA GGAGGTGTCA CCGGAGGCTG CTACGCCACC GGCAACAGAA 
GAGGAGGCAA CGCCGGAGGG CAAGGAGGAC GAGTCCCTGG ATGATGGCGA AATGGCGACC 
AACGCGGGAG CAGCAGCAGC TGCATCACCA TCGCCGGCAG TTCGTGGCAA ATTTCGCTCA 
TCCACCACGG ATTCACCTGA TGAGGGCGCC AGCAGTAGCA TTGCCAGCGG CATTCGTCGT 
GTGGACTTTG AACAGCCGGC TGGGGTCGAG GTGGAGCGAC CAAACAACCT GCCACTGGCG 
GATGCACCAG CTCCACTGAA GCCCGCCCGT CAAAAGTTCA AGGAAAAGTC GCGCGATCAG 
CGACTTTTAT CCGTTCCGAA CATTAAGTAT CAGCCGCGAG ATTCGCGTGG CGGTGCTCGG 
AACCTCAAGA ATGATGTCTC ACCGCTGATG GGCGGTGGTG GCGGTGGAGG AGGCGTTGTT 
GGTGCCAGCA GCGGCATTGG TGGAAGCGTC GCTGGAGGCA AGAAGATACA TAAATTGGAT 
GGAACTTTCT CAAGATCCAG ATCATCGTCA ATGTCCAGCA TTGATATGTC TTCCTCTGAA 
TCTGTTACCT GTTTAGCTTT TATCGAGAGT TATGCAAAAC GAAATGATAT TTTGACACTT 
GTTCCTACTT TGTGGATTGG AACTAGTTTC GGTTCGATAC TGACATTGTT CATCACCATG 
CCCGAGCGAG ATGTGCGCAA ATCTCAGCCA GTATTGATAA CAATAAATGG TGGCCCCGTG 
GTACGACTCA AAGGATCCAT AACTTCCATG TCTTTTTTAG ACTCATACGG TTCGATCATA 
CCCTATACGT TTGAGACCTG GCGTGACGAG AAGAGGGACA ACAAAGATCG CACACCAACA 
AAGAGCAGCA GCCGCACCAG TCCCACATTC ACACCGACAA CTGCCAATAC ATTATCAAAC 
ACTTCGGTCG CGGGTGGTGC GTCCGCAGGC GGAGGAGCTG GCGGCGGTGG TGGTGCCGGC 
GGTGGTGCGG CCAGCGGTGG CGCAGCCGCA GGAGGGCCAG CCGGCGGTGG TGCCGGAGCA 
GGATCGGCGG CTGGAGCGGC CGGCGGCGTT GGAAGCGGTG TAACCAGCGG CGGGGGGATG 
AGCAGCAGCA GCGCCAGCAG CAGCGGCATC AGCGGAAGTG TGAGCACCGG CCTGGAAACT 
CTCACCGATC GACAATACAT CGTTATCGCA AGCGAGAAGC AAACAAAAGT CTTCGATGTC 
GCCAACCAGT GCTGCATCAA CCGCATACAA TTATCGGAAA TGGATTTTGC AGTCAAGGCA 
GAAACTATCA CGATGAAAGA TGGCTCTTGC TTAGCAACCT ATCTTTCGAA TGGCCATCTA 
ATGGTACATA GTCTTCCTAG TCTAAAACTG TTATTGGATA CTGATTTCTT ACCACTTATG 
GAACTAAGTT TTCAAACAAA ATGTAAACAG GGAATTGTGG ATCCAATGCT TTCGATATGG 
GGTCAACAGA TCATTGTTCA TGAAGATACA ACTCAAATAT CAAAAATATT TTGCTTTAGT 
CATAAAGGTC ATGGATTGTA CATGGCATCG CCCACCGAAA TTCAAAAATT CACGATATCA 
TCGGAATTTT GTCAATTTAT TTTGGAGATG ATGGGTGAAC TATATACGGT ACACGAAATG 
CCCGAACAGC CGAAGGAGGG TTTCTTCAAG GGTCTATTTG GTGGTGGCGC CAAACTCCTG 
GATCGCGAGG AGCTATTTGG CGAGCAGAGC GGGAAACCGA ATCGATCGGT GGCACGGCAC 
ATACCCGGCC CCAATTTGGA GCAGCTCGGC CAGAGGGCTT CCACCGCCGC ATCGGAGATC 
AGCCGGGCGC ACCAGCTGGC CATGGAGCGC GGCGAGAAGC TAAATTTGCT GGAGGAGCGC 
GCCGAGCGCA TGGCCAACAC TGCTCAAGAT TTCAGCGGCA CTGCGCATCA ATTAATGCTT 
AAATATAAAG ACAAGAAGTG GTATCAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727628.3
CDS	679..5091
Translation	MKKFTFKGVLDGFRQSVQPQATRQEQEIQEQLKADHFTLKKTFRHGFPYSPTSFAFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTVTADDTIHLWSIRQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYIINWNKAIEVVRTSHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWHYEGKYFVSSHTDGSICSWPTKPQAKPQSQVCPHAKINKDGNAEKCKPIYKVDLKSSATGETFTIFSGGMPSEKGSKSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLIDLLTPGFPCFESPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGEWSPASCSYSEIVITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPESDNPLAVHLIFLCSESRRLCVAGAMGQVMLFKFRKVESTSEVLVLEIPILYENFDDIYGTSPECDFLTGHQVQKAESSDSDKTAECSLKVRNGAQRKPPGFQSQLVCLTTGPNRRNVQVTSLCISSSYGLMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKRIENKEEQSRSPSSDQLNDTTSPDSPSLEFVPEAGETSAHDGVATATSSRPSSPDPVVNSARETLLSGSAAATAAAAASAAVMMAKSPQRDDAAPKELQDRRKSMSWKTFNLKRQLSKVNMKIGGLNVNTDMESLKNSSIFYTPKEVSPEAATPPATEEEATPEGKEDESLDDGEMATNAGAAAAASPSPAVRGKFRSSTTDSPDEGASSSIASGIRRVDFEQPAGVEVERPNNLPLADAPAPLKPARQKFKEKSRDQRLLSVPNIKYQPRDSRGGARNLKNDVSPLMGGGGGGGGVVGASSGIGGSVAGGKKIHKLDGTFSRSRSSSMSSIDMSSSESVTCLAFIESYAKRNDILTLVPTLWIGTSFGSILTLFITMPERDVRKSQPVLITINGGPVVRLKGSITSMSFLDSYGSIIPYTFETWRDEKRDNKDRTPTKSSSRTSPTFTPTTANTLSNTSVAGGASAGGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGGMSSSSASSSGISGSVSTGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETITMKDGSCLATYLSNGHLMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHEDTTQISKIFCFSHKGHGLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFGGGAKLLDREELFGEQSGKPNRSVARHIPGPNLEQLGQRASTAASEISRAHQLAMERGEKLNLLEERAERMANTAQDFSGTAHQLMLKYKDKKWYQL

Target ORF information:

RefSeq Version	NM_167328.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tomosyn, transcript variant I (Tomosyn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167328.4

ORF Insert Sequence:

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGCTGAAC 
GACACGACCA GTCCGGATAG TCCGTCTCTC GAATTTGTAC CGGAGGCTGG CGAAACATCG 
GCCCACGATG GCGTGGCGAC GGCAACTAGC TCACGTCCCA GTTCACCGGA CCCGGTGGTG 
AACAGTGCTC GTGAAACGCT GCTCAGCGGA TCGGCAGCGG CAACGGCGGC TGCGGCAGCA 
TCGGCGGCTG TCATGATGGC CAAGAGTCCC CAGCGGGATG ATGCAGCTCC CAAGGAGCTG 
CAGGACAGAC GAAAGTCCAT GTCCTGGAAA ACGTTCAATT TGAAGCGTCA ATTATCGAAA 
GTCAATATGA AGATCGGCGG CCTGAATGTC AATACCGATA TGGAGTCGCT AAAGAATAGC 
TCCATTTTCT ACACGCCCAA GGAGGTGTCA CCGGAGGCTG CTACGCCACC GGCAACAGAA 
GAGGAGGCAA CGCCGGAGGG CAAGGAGGAC GAGTCCCTGG ATGATGGCGA AATGGCGACC 
AACGCGGGAG CAGCAGCAGC TGCATCACCA TCGCCGGCAG TTCGTGGCAA ATTTCGCTCA 
TCCACCACGG ATTCACCTGA TGAGGGCGCC AGCAGTAGCA TTGCCAGCGG CATTCGTCGT 
GTGGACTTTG AACAGCCGGC TGGGGTCGAG GTGGAGCGAC CAAACAACCT GCCACTGGCG 
GATGCACCAG CTCCACTGAA GCCCGCCCGT CAAAAGTTCA AGGAAAAGTC GCGCGATCAG 
CGACTTTTAT CCGTTCCGAA CATTAAGTAT CAGCCGCGAG ATTCGCGTGG CGGTGCTCGG 
AACCTCAAGA ATGATGTCTC ACCGCTGATG GGCGGTGGTG GCGGTGGAGG AGGCGTTGTT 
GGTGCCAGCA GCGGCATTGG TGGAAGCGTC GCTGGAGGCA AGAAGATACA TAAATTGGAT 
GGAACTTTCT CAAGATCCAG ATCATCGTCA ATGTCCAGCA TTGATATGTC TTCCTCTGAA 
TCTGTTACCT GTTTAGCTTT TATCGAGAGT TATGCAAAAC GAAATGATAT TTTGACACTT 
GTTCCTACTT TGTGGATTGG AACTAGTTTC GGTTCGATAC TGACATTGTT CATCACCATG 
CCCGAGCGAG ATGTGCGCAA ATCTCAGCCA GTATTGATAA CAATAAATGG TGGCCCCGTG 
GTACGACTCA AAGGATCCAT AACTTCCATG TCTTTTTTAG ACTCATACGG TTCGATCATA 
CCCTATACGT TTGAGACCTG GCGTGACGAG AAGAGGGACA ACAAAGATCG CACACCAACA 
AAGAGCAGCA GCCGCACCAG TCCCACATTC ACACCGACAA CTGCCAATAC ATTATCAAAC 
ACTTCGGTCG CGGGTGGTGC GTCCGCAGGC GGAGGAGCTG GCGGCGGTGG TGGTGCCGGC 
GGTGGTGCGG CCAGCGGTGG CGCAGCCGCA GGAGGGCCAG CCGGCGGTGG TGCCGGAGCA 
GGATCGGCGG CTGGAGCGGC CGGCGGCGTT GGAAGCGGTG TAACCAGCGG CGGGGGGATG 
AGCAGCAGCA GCGCCAGCAG CAGCGGCATC AGCGGAAGTG TGAGCACCGG CCTGGAAACT 
CTCACCGATC GACAATACAT CGTTATCGCA AGCGAGAAGC AAACAAAAGT CTTCGATGTC 
GCCAACCAGT GCTGCATCAA CCGCATACAA TTATCGGAAA TGGATTTTGC AGTCAAGGCA 
GAAACTATCA CGATGAAAGA TGGCTCTTGC TTAGCAACCT ATCTTTCGAA TGGCCATCTA 
ATGGTACATA GTCTTCCTAG TCTAAAACTG TTATTGGATA CTGATTTCTT ACCACTTATG 
GAACTAAGTT TTCAAACAAA ATGTAAACAG GGAATTGTGG ATCCAATGCT TTCGATATGG 
GGTCAACAGA TCATTGTTCA TGAAGATACA ACTCAAATAT CAAAAATATT TTGCTTTAGT 
CATAAAGGTC ATGGATTGTA CATGGCATCG CCCACCGAAA TTCAAAAATT CACGATATCA 
TCGGAATTTT GTCAATTTAT TTTGGAGATG ATGGGTGAAC TATATACGGT ACACGAAATG 
CCCGAACAGC CGAAGGAGGG TTTCTTCAAG GGTCTATTTG GTGGTGGCGC CAAACTCCTG 
GATCGCGAGG AGCTATTTGG CGAGCAGAGC GGGAAACCGA ATCGATCGGT GGCACGGCAC 
ATACCCGGCC CCAATTTGGA GCAGCTCGGC CAGAGGGCTT CCACCGCCGC ATCGGAGATC 
AGCCGGGCGC ACCAGCTGGC CATGGAGCGC GGCGAGAAGC TAAATTTGCT GGAGGAGCGC 
GCCGAGCGCA TGGCCAACAC TGCTCAAGAT TTCAGCGGCA CTGCGCATCA ATTAATGCTT 
AAATATAAAG ACAAGAAGTG GTATCAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tomosyn ( NM_001258715.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245644.1 Drosophila melanogaster tomosyn, transcript variant H (Tomosyn), mRNA.

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CloneID	OFa01419
Clone ID Related Accession (Same CDS sequence)	NM_001258715.2 , NM_132591.5
Accession Version	NM_001258715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tomosyn, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258715.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGATCAAC 
GGCAGCGGAG TTGCAACTGC AGCACCGCAG CAGCAACAGC AGCAGCAGCA ACAGTTGCAG 
CAATCACAGC AACAACAGCA GCAGCAGCTA CAGCAGCAAC AGCAGCAGCA GCAACAACAA 
CAGCAGCAGC AGCAACAACA ACAACAATCG TTAGGTGGTG ATCCTGGTGA TCATGCAACA 
TCGACGTCAG CGGGAGCAGC AACATTGGCG GGATCCTCCA TTTACGGTGC AGATGATGCA 
GATAAATTGG ATGGAACTTT CTCAAGATCC AGATCATCGT CAATGTCCAG CATTGATATG 
TCTTCCTCTG AATCTGTTAC CTGTTTAGCT TTTATCGAGA GTTATGCAAA ACGAAATGAT 
ATTTTGACAC TTGTTCCTAC TTTGTGGATT GGAACTAGTT TCGGTTCGAT ACTGACATTG 
TTCATCACCA TGCCCGAGCG AGATGTGCGC AAATCTCAGC CAGTATTGAT AACAATAAAT 
GGTGGCCCCG TGGTACGACT CAAAGGATCC ATAACTTCCA TGTCTTTTTT AGACTCATAC 
GGTTCGATCA TACCCTATAC GTTTGAGACC TGGCGTGACG AGAAGAGGGA CAACAAAGAT 
CGCACACCAA CAAAGAGCAG CAGCCGCACC AGTCCCACAT TCACACCGAC AACTGCCAAT 
ACATTATCAA ACACTTCGGT CGCGGGTGGT GCGTCCGCAG GCGGAGGAGC TGGCGGCGGT 
GGTGGTGCCG GCGGTGGTGC GGCCAGCGGT GGCGCAGCCG CAGGAGGGCC AGCCGGCGGT 
GGTGCCGGAG CAGGATCGGC GGCTGGAGCG GCCGGCGGCG TTGGAAGCGG TGTAACCAGC 
GGCGGGGGGA TGAGCAGCAG CAGCGCCAGC AGCAGCGGCA TCAGCGGAAG TGTGAGCACC 
GGCCTGGAAA CTCTCACCGA TCGACAATAC ATCGTTATCG CAAGCGAGAA GCAAACAAAA 
GTCTTCGATG TCGCCAACCA GTGCTGCATC AACCGCATAC AATTATCGGA AATGGATTTT 
GCAGTCAAGG CAGAAACTAT CACGATGAAA GATGGCTCTT GCTTAGCAAC CTATCTTTCG 
AATGGCCATC TAATGGTACA TAGTCTTCCT AGTCTAAAAC TGTTATTGGA TACTGATTTC 
TTACCACTTA TGGAACTAAG TTTTCAAACA AAATGTAAAC AGGGAATTGT GGATCCAATG 
CTTTCGATAT GGGGTCAACA GATCATTGTT CATGAAGATA CAACTCAAAT ATCAAAAATA 
TTTTGCTTTA GTCATAAAGG TCATGGATTG TACATGGCAT CGCCCACCGA AATTCAAAAA 
TTCACGATAT CATCGGAATT TTGTCAATTT ATTTTGGAGA TGATGGGTGA ACTATATACG 
GTACACGAAA TGCCCGAACA GCCGAAGGAG GGTTTCTTCA AGGGTCTATT TGGTGGTGGC 
GCCAAACTCC TGGATCGCGA GGAGCTATTT GGCGAGCAGA GCGGGAAACC GAATCGATCG 
GTGGCACGGC ACATACCCGG CCCCAATTTG GAGCAGCTCG GCCAGAGGGC TTCCACCGCC 
GCATCGGAGA TCAGCCGGGC GCACCAGCTG GCCATGGAGC GCGGCGAGAA GCTAAATTTG 
CTGGAGGAGC GCGCCGAGCG CATGGCCAAC ACTGCTCAAG ATTTCAGCGG CACTGCGCAT 
CAATTAATGC TTAAATATAA AGACAAGAAG TGGTATCAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245644.1
CDS	639..4403
Translation	MKKFTFKGVLDGFRQSVQPQATRQEQEIQEQLKADHFTLKKTFRHGFPYSPTSFAFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTVTADDTIHLWSIRQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYIINWNKAIEVVRTSHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWHYEGKYFVSSHTDGSICSWPTKPQAKPQSQVCPHAKINKDGNAEKCKPIYKVDLKSSATGETFTIFSGGMPSEKGSKSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLIDLLTPGFPCFESPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGEWSPASCSYSEIVITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPESDNPLAVHLIFLCSESRRLCVAGAMGQVMLFKFRKVESTSEVLVLEIPILYENFDDIYGTSPECDFLTGHQVQKAESSDSDKTAECSLKVRNGAQRKPPGFQSQLVCLTTGPNRRNVQVTSLCISSSYGLMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKRIENKEEQSRSPSSDQINGSGVATAAPQQQQQQQQQLQQSQQQQQQQLQQQQQQQQQQQQQQQQQQQSLGGDPGDHATSTSAGAATLAGSSIYGADDADKLDGTFSRSRSSSMSSIDMSSSESVTCLAFIESYAKRNDILTLVPTLWIGTSFGSILTLFITMPERDVRKSQPVLITINGGPVVRLKGSITSMSFLDSYGSIIPYTFETWRDEKRDNKDRTPTKSSSRTSPTFTPTTANTLSNTSVAGGASAGGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGGMSSSSASSSGISGSVSTGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETITMKDGSCLATYLSNGHLMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHEDTTQISKIFCFSHKGHGLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFGGGAKLLDREELFGEQSGKPNRSVARHIPGPNLEQLGQRASTAASEISRAHQLAMERGEKLNLLEERAERMANTAQDFSGTAHQLMLKYKDKKWYQL

Target ORF information:

RefSeq Version	NM_001258715.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tomosyn, transcript variant H (Tomosyn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258715.2

ORF Insert Sequence:

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGATCAAC 
GGCAGCGGAG TTGCAACTGC AGCACCGCAG CAGCAACAGC AGCAGCAGCA ACAGTTGCAG 
CAATCACAGC AACAACAGCA GCAGCAGCTA CAGCAGCAAC AGCAGCAGCA GCAACAACAA 
CAGCAGCAGC AGCAACAACA ACAACAATCG TTAGGTGGTG ATCCTGGTGA TCATGCAACA 
TCGACGTCAG CGGGAGCAGC AACATTGGCG GGATCCTCCA TTTACGGTGC AGATGATGCA 
GATAAATTGG ATGGAACTTT CTCAAGATCC AGATCATCGT CAATGTCCAG CATTGATATG 
TCTTCCTCTG AATCTGTTAC CTGTTTAGCT TTTATCGAGA GTTATGCAAA ACGAAATGAT 
ATTTTGACAC TTGTTCCTAC TTTGTGGATT GGAACTAGTT TCGGTTCGAT ACTGACATTG 
TTCATCACCA TGCCCGAGCG AGATGTGCGC AAATCTCAGC CAGTATTGAT AACAATAAAT 
GGTGGCCCCG TGGTACGACT CAAAGGATCC ATAACTTCCA TGTCTTTTTT AGACTCATAC 
GGTTCGATCA TACCCTATAC GTTTGAGACC TGGCGTGACG AGAAGAGGGA CAACAAAGAT 
CGCACACCAA CAAAGAGCAG CAGCCGCACC AGTCCCACAT TCACACCGAC AACTGCCAAT 
ACATTATCAA ACACTTCGGT CGCGGGTGGT GCGTCCGCAG GCGGAGGAGC TGGCGGCGGT 
GGTGGTGCCG GCGGTGGTGC GGCCAGCGGT GGCGCAGCCG CAGGAGGGCC AGCCGGCGGT 
GGTGCCGGAG CAGGATCGGC GGCTGGAGCG GCCGGCGGCG TTGGAAGCGG TGTAACCAGC 
GGCGGGGGGA TGAGCAGCAG CAGCGCCAGC AGCAGCGGCA TCAGCGGAAG TGTGAGCACC 
GGCCTGGAAA CTCTCACCGA TCGACAATAC ATCGTTATCG CAAGCGAGAA GCAAACAAAA 
GTCTTCGATG TCGCCAACCA GTGCTGCATC AACCGCATAC AATTATCGGA AATGGATTTT 
GCAGTCAAGG CAGAAACTAT CACGATGAAA GATGGCTCTT GCTTAGCAAC CTATCTTTCG 
AATGGCCATC TAATGGTACA TAGTCTTCCT AGTCTAAAAC TGTTATTGGA TACTGATTTC 
TTACCACTTA TGGAACTAAG TTTTCAAACA AAATGTAAAC AGGGAATTGT GGATCCAATG 
CTTTCGATAT GGGGTCAACA GATCATTGTT CATGAAGATA CAACTCAAAT ATCAAAAATA 
TTTTGCTTTA GTCATAAAGG TCATGGATTG TACATGGCAT CGCCCACCGA AATTCAAAAA 
TTCACGATAT CATCGGAATT TTGTCAATTT ATTTTGGAGA TGATGGGTGA ACTATATACG 
GTACACGAAA TGCCCGAACA GCCGAAGGAG GGTTTCTTCA AGGGTCTATT TGGTGGTGGC 
GCCAAACTCC TGGATCGCGA GGAGCTATTT GGCGAGCAGA GCGGGAAACC GAATCGATCG 
GTGGCACGGC ACATACCCGG CCCCAATTTG GAGCAGCTCG GCCAGAGGGC TTCCACCGCC 
GCATCGGAGA TCAGCCGGGC GCACCAGCTG GCCATGGAGC GCGGCGAGAA GCTAAATTTG 
CTGGAGGAGC GCGCCGAGCG CATGGCCAAC ACTGCTCAAG ATTTCAGCGG CACTGCGCAT 
CAATTAATGC TTAAATATAA AGACAAGAAG TGGTATCAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tomosyn ( NM_132591.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572819.4 Drosophila melanogaster tomosyn, transcript variant J (Tomosyn), mRNA.

Price & Availability↑

CloneID	OFa01419
Clone ID Related Accession (Same CDS sequence)	NM_001258715.2 , NM_132591.5
Accession Version	NM_132591.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tomosyn, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132591.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGATCAAC 
GGCAGCGGAG TTGCAACTGC AGCACCGCAG CAGCAACAGC AGCAGCAGCA ACAGTTGCAG 
CAATCACAGC AACAACAGCA GCAGCAGCTA CAGCAGCAAC AGCAGCAGCA GCAACAACAA 
CAGCAGCAGC AGCAACAACA ACAACAATCG TTAGGTGGTG ATCCTGGTGA TCATGCAACA 
TCGACGTCAG CGGGAGCAGC AACATTGGCG GGATCCTCCA TTTACGGTGC AGATGATGCA 
GATAAATTGG ATGGAACTTT CTCAAGATCC AGATCATCGT CAATGTCCAG CATTGATATG 
TCTTCCTCTG AATCTGTTAC CTGTTTAGCT TTTATCGAGA GTTATGCAAA ACGAAATGAT 
ATTTTGACAC TTGTTCCTAC TTTGTGGATT GGAACTAGTT TCGGTTCGAT ACTGACATTG 
TTCATCACCA TGCCCGAGCG AGATGTGCGC AAATCTCAGC CAGTATTGAT AACAATAAAT 
GGTGGCCCCG TGGTACGACT CAAAGGATCC ATAACTTCCA TGTCTTTTTT AGACTCATAC 
GGTTCGATCA TACCCTATAC GTTTGAGACC TGGCGTGACG AGAAGAGGGA CAACAAAGAT 
CGCACACCAA CAAAGAGCAG CAGCCGCACC AGTCCCACAT TCACACCGAC AACTGCCAAT 
ACATTATCAA ACACTTCGGT CGCGGGTGGT GCGTCCGCAG GCGGAGGAGC TGGCGGCGGT 
GGTGGTGCCG GCGGTGGTGC GGCCAGCGGT GGCGCAGCCG CAGGAGGGCC AGCCGGCGGT 
GGTGCCGGAG CAGGATCGGC GGCTGGAGCG GCCGGCGGCG TTGGAAGCGG TGTAACCAGC 
GGCGGGGGGA TGAGCAGCAG CAGCGCCAGC AGCAGCGGCA TCAGCGGAAG TGTGAGCACC 
GGCCTGGAAA CTCTCACCGA TCGACAATAC ATCGTTATCG CAAGCGAGAA GCAAACAAAA 
GTCTTCGATG TCGCCAACCA GTGCTGCATC AACCGCATAC AATTATCGGA AATGGATTTT 
GCAGTCAAGG CAGAAACTAT CACGATGAAA GATGGCTCTT GCTTAGCAAC CTATCTTTCG 
AATGGCCATC TAATGGTACA TAGTCTTCCT AGTCTAAAAC TGTTATTGGA TACTGATTTC 
TTACCACTTA TGGAACTAAG TTTTCAAACA AAATGTAAAC AGGGAATTGT GGATCCAATG 
CTTTCGATAT GGGGTCAACA GATCATTGTT CATGAAGATA CAACTCAAAT ATCAAAAATA 
TTTTGCTTTA GTCATAAAGG TCATGGATTG TACATGGCAT CGCCCACCGA AATTCAAAAA 
TTCACGATAT CATCGGAATT TTGTCAATTT ATTTTGGAGA TGATGGGTGA ACTATATACG 
GTACACGAAA TGCCCGAACA GCCGAAGGAG GGTTTCTTCA AGGGTCTATT TGGTGGTGGC 
GCCAAACTCC TGGATCGCGA GGAGCTATTT GGCGAGCAGA GCGGGAAACC GAATCGATCG 
GTGGCACGGC ACATACCCGG CCCCAATTTG GAGCAGCTCG GCCAGAGGGC TTCCACCGCC 
GCATCGGAGA TCAGCCGGGC GCACCAGCTG GCCATGGAGC GCGGCGAGAA GCTAAATTTG 
CTGGAGGAGC GCGCCGAGCG CATGGCCAAC ACTGCTCAAG ATTTCAGCGG CACTGCGCAT 
CAATTAATGC TTAAATATAA AGACAAGAAG TGGTATCAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572819.4
CDS	684..4448
Translation	MKKFTFKGVLDGFRQSVQPQATRQEQEIQEQLKADHFTLKKTFRHGFPYSPTSFAFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTVTADDTIHLWSIRQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYIINWNKAIEVVRTSHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWHYEGKYFVSSHTDGSICSWPTKPQAKPQSQVCPHAKINKDGNAEKCKPIYKVDLKSSATGETFTIFSGGMPSEKGSKSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLIDLLTPGFPCFESPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGEWSPASCSYSEIVITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPESDNPLAVHLIFLCSESRRLCVAGAMGQVMLFKFRKVESTSEVLVLEIPILYENFDDIYGTSPECDFLTGHQVQKAESSDSDKTAECSLKVRNGAQRKPPGFQSQLVCLTTGPNRRNVQVTSLCISSSYGLMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKRIENKEEQSRSPSSDQINGSGVATAAPQQQQQQQQQLQQSQQQQQQQLQQQQQQQQQQQQQQQQQQQSLGGDPGDHATSTSAGAATLAGSSIYGADDADKLDGTFSRSRSSSMSSIDMSSSESVTCLAFIESYAKRNDILTLVPTLWIGTSFGSILTLFITMPERDVRKSQPVLITINGGPVVRLKGSITSMSFLDSYGSIIPYTFETWRDEKRDNKDRTPTKSSSRTSPTFTPTTANTLSNTSVAGGASAGGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGGMSSSSASSSGISGSVSTGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETITMKDGSCLATYLSNGHLMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHEDTTQISKIFCFSHKGHGLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFGGGAKLLDREELFGEQSGKPNRSVARHIPGPNLEQLGQRASTAASEISRAHQLAMERGEKLNLLEERAERMANTAQDFSGTAHQLMLKYKDKKWYQL

Target ORF information:

RefSeq Version	NM_132591.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tomosyn, transcript variant J (Tomosyn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132591.5

ORF Insert Sequence:

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGATCAAC 
GGCAGCGGAG TTGCAACTGC AGCACCGCAG CAGCAACAGC AGCAGCAGCA ACAGTTGCAG 
CAATCACAGC AACAACAGCA GCAGCAGCTA CAGCAGCAAC AGCAGCAGCA GCAACAACAA 
CAGCAGCAGC AGCAACAACA ACAACAATCG TTAGGTGGTG ATCCTGGTGA TCATGCAACA 
TCGACGTCAG CGGGAGCAGC AACATTGGCG GGATCCTCCA TTTACGGTGC AGATGATGCA 
GATAAATTGG ATGGAACTTT CTCAAGATCC AGATCATCGT CAATGTCCAG CATTGATATG 
TCTTCCTCTG AATCTGTTAC CTGTTTAGCT TTTATCGAGA GTTATGCAAA ACGAAATGAT 
ATTTTGACAC TTGTTCCTAC TTTGTGGATT GGAACTAGTT TCGGTTCGAT ACTGACATTG 
TTCATCACCA TGCCCGAGCG AGATGTGCGC AAATCTCAGC CAGTATTGAT AACAATAAAT 
GGTGGCCCCG TGGTACGACT CAAAGGATCC ATAACTTCCA TGTCTTTTTT AGACTCATAC 
GGTTCGATCA TACCCTATAC GTTTGAGACC TGGCGTGACG AGAAGAGGGA CAACAAAGAT 
CGCACACCAA CAAAGAGCAG CAGCCGCACC AGTCCCACAT TCACACCGAC AACTGCCAAT 
ACATTATCAA ACACTTCGGT CGCGGGTGGT GCGTCCGCAG GCGGAGGAGC TGGCGGCGGT 
GGTGGTGCCG GCGGTGGTGC GGCCAGCGGT GGCGCAGCCG CAGGAGGGCC AGCCGGCGGT 
GGTGCCGGAG CAGGATCGGC GGCTGGAGCG GCCGGCGGCG TTGGAAGCGG TGTAACCAGC 
GGCGGGGGGA TGAGCAGCAG CAGCGCCAGC AGCAGCGGCA TCAGCGGAAG TGTGAGCACC 
GGCCTGGAAA CTCTCACCGA TCGACAATAC ATCGTTATCG CAAGCGAGAA GCAAACAAAA 
GTCTTCGATG TCGCCAACCA GTGCTGCATC AACCGCATAC AATTATCGGA AATGGATTTT 
GCAGTCAAGG CAGAAACTAT CACGATGAAA GATGGCTCTT GCTTAGCAAC CTATCTTTCG 
AATGGCCATC TAATGGTACA TAGTCTTCCT AGTCTAAAAC TGTTATTGGA TACTGATTTC 
TTACCACTTA TGGAACTAAG TTTTCAAACA AAATGTAAAC AGGGAATTGT GGATCCAATG 
CTTTCGATAT GGGGTCAACA GATCATTGTT CATGAAGATA CAACTCAAAT ATCAAAAATA 
TTTTGCTTTA GTCATAAAGG TCATGGATTG TACATGGCAT CGCCCACCGA AATTCAAAAA 
TTCACGATAT CATCGGAATT TTGTCAATTT ATTTTGGAGA TGATGGGTGA ACTATATACG 
GTACACGAAA TGCCCGAACA GCCGAAGGAG GGTTTCTTCA AGGGTCTATT TGGTGGTGGC 
GCCAAACTCC TGGATCGCGA GGAGCTATTT GGCGAGCAGA GCGGGAAACC GAATCGATCG 
GTGGCACGGC ACATACCCGG CCCCAATTTG GAGCAGCTCG GCCAGAGGGC TTCCACCGCC 
GCATCGGAGA TCAGCCGGGC GCACCAGCTG GCCATGGAGC GCGGCGAGAA GCTAAATTTG 
CTGGAGGAGC GCGCCGAGCG CATGGCCAAC ACTGCTCAAG ATTTCAGCGG CACTGCGCAT 
CAATTAATGC TTAAATATAA AGACAAGAAG TGGTATCAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tomosyn ( NM_001169265.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162736.2 Drosophila melanogaster tomosyn, transcript variant K (Tomosyn), mRNA.

Price & Availability↑

CloneID	OFa21448
Clone ID Related Accession (Same CDS sequence)	NM_001169265.2 , NM_167330.3
Accession Version	NM_001169265.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tomosyn, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001169265.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGATCAAC 
GGCAGCGGAG TTGCAACTGC AGCACCGCAG CAGCAACAGC AGCAGCAGCA ACAGTTGCAG 
CAATCACAGC AACAACAGCA GCAGCAGCTA CAGCAGCAAC AGCAGCAGCA GCAACAACAA 
CAGCAGCAGC AGCAACAACA ACAACAATCG TTAGGTGGTG ATCCTGGTGA TCATGCAACA 
TCGACGTCAG CGGGAGCAGC AACATTGGCG GGATCCTCCA TTTACGGTGC AGATGATGCA 
GGTGCGGGTG TTGCTAGTGG CGGTGTTGGT GTTGGTGGTA TTAGCAGTGC GATTAGCGGT 
GGATTTGGTG GTGCGGCTGA GCCGCAACAG CAGCAACAGG TGTCGCAACA GTTGCATCAA 
TTACAGCAAC TACAGCAGCA ATCGCAGCAG CAGCAGCAAT CGTTTGAGGC GTCTTGTGAT 
CCATGGGGCG GCGAGTGCGG CGGTGGTGGT GCTGGCGGCA GCGGCGGCGG CGGTGGTGCA 
AGCGGTGGTG GTGGTGCTAG CGGTGCAGGT GCGCAACAAC ATCAATATCA GCATCCGCAT 
CCGTATCCGC ATCCGCATCC GTATCCGCAT CAAACATCCG TTATACAGCA ACAGCAGTCA 
GTGACATCGT ACGAATCCCT AAAAAAGTCC AAATCACAAG ATAAATTGGA TGGAACTTTC 
TCAAGATCCA GATCATCGTC AATGTCCAGC ATTGATATGT CTTCCTCTGA ATCTGTTACC 
TGTTTAGCTT TTATCGAGAG TTATGCAAAA CGAAATGATA TTTTGACACT TGTTCCTACT 
TTGTGGATTG GAACTAGTTT CGGTTCGATA CTGACATTGT TCATCACCAT GCCCGAGCGA 
GATGTGCGCA AATCTCAGCC AGTATTGATA ACAATAAATG GTGGCCCCGT GGTACGACTC 
AAAGGATCCA TAACTTCCAT GTCTTTTTTA GACTCATACG GTTCGATCAT ACCCTATACG 
TTTGAGACCT GGCGTGACGA GAAGAGGGAC AACAAAGATC GCACACCAAC AAAGAGCAGC 
AGCCGCACCA GTCCCACATT CACACCGACA ACTGCCAATA CATTATCAAA CACTTCGGTC 
GCGGGTGGTG CGTCCGCAGG CGGAGGAGCT GGCGGCGGTG GTGGTGCCGG CGGTGGTGCG 
GCCAGCGGTG GCGCAGCCGC AGGAGGGCCA GCCGGCGGTG GTGCCGGAGC AGGATCGGCG 
GCTGGAGCGG CCGGCGGCGT TGGAAGCGGT GTAACCAGCG GCGGGGGGAT GAGCAGCAGC 
AGCGCCAGCA GCAGCGGCAT CAGCGGAAGT GTGAGCACCG GCCTGGAAAC TCTCACCGAT 
CGACAATACA TCGTTATCGC AAGCGAGAAG CAAACAAAAG TCTTCGATGT CGCCAACCAG 
TGCTGCATCA ACCGCATACA ATTATCGGAA ATGGATTTTG CAGTCAAGGC AGAAACTATC 
ACGATGAAAG ATGGCTCTTG CTTAGCAACC TATCTTTCGA ATGGCCATCT AATGGTACAT 
AGTCTTCCTA GTCTAAAACT GTTATTGGAT ACTGATTTCT TACCACTTAT GGAACTAAGT 
TTTCAAACAA AATGTAAACA GGGAATTGTG GATCCAATGC TTTCGATATG GGGTCAACAG 
ATCATTGTTC ATGAAGATAC AACTCAAATA TCAAAAATAT TTTGCTTTAG TCATAAAGGT 
CATGGATTGT ACATGGCATC GCCCACCGAA ATTCAAAAAT TCACGATATC ATCGGAATTT 
TGTCAATTTA TTTTGGAGAT GATGGGTGAA CTATATACGG TACACGAAAT GCCCGAACAG 
CCGAAGGAGG GTTTCTTCAA GGGTCTATTT GGTGGTGGCG CCAAACTCCT GGATCGCGAG 
GAGCTATTTG GCGAGCAGAG CGGGAAACCG AATCGATCGG TGGCACGGCA CATACCCGGC 
CCCAATTTGG AGCAGCTCGG CCAGAGGGCT TCCACCGCCG CATCGGAGAT CAGCCGGGCG 
CACCAGCTGG CCATGGAGCG CGGCGAGAAG CTAAATTTGC TGGAGGAGCG CGCCGAGCGC 
ATGGCCAACA CTGCTCAAGA TTTCAGCGGC ACTGCGCATC AATTAATGCT TAAATATAAA 
GACAAGAAGT GGTATCAGTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162736.2
CDS	639..4802
Translation	MKKFTFKGVLDGFRQSVQPQATRQEQEIQEQLKADHFTLKKTFRHGFPYSPTSFAFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTVTADDTIHLWSIRQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYIINWNKAIEVVRTSHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWHYEGKYFVSSHTDGSICSWPTKPQAKPQSQVCPHAKINKDGNAEKCKPIYKVDLKSSATGETFTIFSGGMPSEKGSKSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLIDLLTPGFPCFESPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGEWSPASCSYSEIVITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPESDNPLAVHLIFLCSESRRLCVAGAMGQVMLFKFRKVESTSEVLVLEIPILYENFDDIYGTSPECDFLTGHQVQKAESSDSDKTAECSLKVRNGAQRKPPGFQSQLVCLTTGPNRRNVQVTSLCISSSYGLMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKRIENKEEQSRSPSSDQINGSGVATAAPQQQQQQQQQLQQSQQQQQQQLQQQQQQQQQQQQQQQQQQQSLGGDPGDHATSTSAGAATLAGSSIYGADDAGAGVASGGVGVGGISSAISGGFGGAAEPQQQQQVSQQLHQLQQLQQQSQQQQQSFEASCDPWGGECGGGGAGGSGGGGGASGGGGASGAGAQQHQYQHPHPYPHPHPYPHQTSVIQQQQSVTSYESLKKSKSQDKLDGTFSRSRSSSMSSIDMSSSESVTCLAFIESYAKRNDILTLVPTLWIGTSFGSILTLFITMPERDVRKSQPVLITINGGPVVRLKGSITSMSFLDSYGSIIPYTFETWRDEKRDNKDRTPTKSSSRTSPTFTPTTANTLSNTSVAGGASAGGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGGMSSSSASSSGISGSVSTGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETITMKDGSCLATYLSNGHLMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHEDTTQISKIFCFSHKGHGLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFGGGAKLLDREELFGEQSGKPNRSVARHIPGPNLEQLGQRASTAASEISRAHQLAMERGEKLNLLEERAERMANTAQDFSGTAHQLMLKYKDKKWYQL

Target ORF information:

RefSeq Version	NM_001169265.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tomosyn, transcript variant K (Tomosyn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169265.2

ORF Insert Sequence:

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGATCAAC 
GGCAGCGGAG TTGCAACTGC AGCACCGCAG CAGCAACAGC AGCAGCAGCA ACAGTTGCAG 
CAATCACAGC AACAACAGCA GCAGCAGCTA CAGCAGCAAC AGCAGCAGCA GCAACAACAA 
CAGCAGCAGC AGCAACAACA ACAACAATCG TTAGGTGGTG ATCCTGGTGA TCATGCAACA 
TCGACGTCAG CGGGAGCAGC AACATTGGCG GGATCCTCCA TTTACGGTGC AGATGATGCA 
GGTGCGGGTG TTGCTAGTGG CGGTGTTGGT GTTGGTGGTA TTAGCAGTGC GATTAGCGGT 
GGATTTGGTG GTGCGGCTGA GCCGCAACAG CAGCAACAGG TGTCGCAACA GTTGCATCAA 
TTACAGCAAC TACAGCAGCA ATCGCAGCAG CAGCAGCAAT CGTTTGAGGC GTCTTGTGAT 
CCATGGGGCG GCGAGTGCGG CGGTGGTGGT GCTGGCGGCA GCGGCGGCGG CGGTGGTGCA 
AGCGGTGGTG GTGGTGCTAG CGGTGCAGGT GCGCAACAAC ATCAATATCA GCATCCGCAT 
CCGTATCCGC ATCCGCATCC GTATCCGCAT CAAACATCCG TTATACAGCA ACAGCAGTCA 
GTGACATCGT ACGAATCCCT AAAAAAGTCC AAATCACAAG ATAAATTGGA TGGAACTTTC 
TCAAGATCCA GATCATCGTC AATGTCCAGC ATTGATATGT CTTCCTCTGA ATCTGTTACC 
TGTTTAGCTT TTATCGAGAG TTATGCAAAA CGAAATGATA TTTTGACACT TGTTCCTACT 
TTGTGGATTG GAACTAGTTT CGGTTCGATA CTGACATTGT TCATCACCAT GCCCGAGCGA 
GATGTGCGCA AATCTCAGCC AGTATTGATA ACAATAAATG GTGGCCCCGT GGTACGACTC 
AAAGGATCCA TAACTTCCAT GTCTTTTTTA GACTCATACG GTTCGATCAT ACCCTATACG 
TTTGAGACCT GGCGTGACGA GAAGAGGGAC AACAAAGATC GCACACCAAC AAAGAGCAGC 
AGCCGCACCA GTCCCACATT CACACCGACA ACTGCCAATA CATTATCAAA CACTTCGGTC 
GCGGGTGGTG CGTCCGCAGG CGGAGGAGCT GGCGGCGGTG GTGGTGCCGG CGGTGGTGCG 
GCCAGCGGTG GCGCAGCCGC AGGAGGGCCA GCCGGCGGTG GTGCCGGAGC AGGATCGGCG 
GCTGGAGCGG CCGGCGGCGT TGGAAGCGGT GTAACCAGCG GCGGGGGGAT GAGCAGCAGC 
AGCGCCAGCA GCAGCGGCAT CAGCGGAAGT GTGAGCACCG GCCTGGAAAC TCTCACCGAT 
CGACAATACA TCGTTATCGC AAGCGAGAAG CAAACAAAAG TCTTCGATGT CGCCAACCAG 
TGCTGCATCA ACCGCATACA ATTATCGGAA ATGGATTTTG CAGTCAAGGC AGAAACTATC 
ACGATGAAAG ATGGCTCTTG CTTAGCAACC TATCTTTCGA ATGGCCATCT AATGGTACAT 
AGTCTTCCTA GTCTAAAACT GTTATTGGAT ACTGATTTCT TACCACTTAT GGAACTAAGT 
TTTCAAACAA AATGTAAACA GGGAATTGTG GATCCAATGC TTTCGATATG GGGTCAACAG 
ATCATTGTTC ATGAAGATAC AACTCAAATA TCAAAAATAT TTTGCTTTAG TCATAAAGGT 
CATGGATTGT ACATGGCATC GCCCACCGAA ATTCAAAAAT TCACGATATC ATCGGAATTT 
TGTCAATTTA TTTTGGAGAT GATGGGTGAA CTATATACGG TACACGAAAT GCCCGAACAG 
CCGAAGGAGG GTTTCTTCAA GGGTCTATTT GGTGGTGGCG CCAAACTCCT GGATCGCGAG 
GAGCTATTTG GCGAGCAGAG CGGGAAACCG AATCGATCGG TGGCACGGCA CATACCCGGC 
CCCAATTTGG AGCAGCTCGG CCAGAGGGCT TCCACCGCCG CATCGGAGAT CAGCCGGGCG 
CACCAGCTGG CCATGGAGCG CGGCGAGAAG CTAAATTTGC TGGAGGAGCG CGCCGAGCGC 
ATGGCCAACA CTGCTCAAGA TTTCAGCGGC ACTGCGCATC AATTAATGCT TAAATATAAA 
GACAAGAAGT GGTATCAGTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tomosyn ( NM_167330.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727630.3 Drosophila melanogaster tomosyn, transcript variant L (Tomosyn), mRNA.

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CloneID	OFa21448
Clone ID Related Accession (Same CDS sequence)	NM_001169265.2 , NM_167330.3
Accession Version	NM_167330.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tomosyn, isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167330.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGATCAAC 
GGCAGCGGAG TTGCAACTGC AGCACCGCAG CAGCAACAGC AGCAGCAGCA ACAGTTGCAG 
CAATCACAGC AACAACAGCA GCAGCAGCTA CAGCAGCAAC AGCAGCAGCA GCAACAACAA 
CAGCAGCAGC AGCAACAACA ACAACAATCG TTAGGTGGTG ATCCTGGTGA TCATGCAACA 
TCGACGTCAG CGGGAGCAGC AACATTGGCG GGATCCTCCA TTTACGGTGC AGATGATGCA 
GGTGCGGGTG TTGCTAGTGG CGGTGTTGGT GTTGGTGGTA TTAGCAGTGC GATTAGCGGT 
GGATTTGGTG GTGCGGCTGA GCCGCAACAG CAGCAACAGG TGTCGCAACA GTTGCATCAA 
TTACAGCAAC TACAGCAGCA ATCGCAGCAG CAGCAGCAAT CGTTTGAGGC GTCTTGTGAT 
CCATGGGGCG GCGAGTGCGG CGGTGGTGGT GCTGGCGGCA GCGGCGGCGG CGGTGGTGCA 
AGCGGTGGTG GTGGTGCTAG CGGTGCAGGT GCGCAACAAC ATCAATATCA GCATCCGCAT 
CCGTATCCGC ATCCGCATCC GTATCCGCAT CAAACATCCG TTATACAGCA ACAGCAGTCA 
GTGACATCGT ACGAATCCCT AAAAAAGTCC AAATCACAAG ATAAATTGGA TGGAACTTTC 
TCAAGATCCA GATCATCGTC AATGTCCAGC ATTGATATGT CTTCCTCTGA ATCTGTTACC 
TGTTTAGCTT TTATCGAGAG TTATGCAAAA CGAAATGATA TTTTGACACT TGTTCCTACT 
TTGTGGATTG GAACTAGTTT CGGTTCGATA CTGACATTGT TCATCACCAT GCCCGAGCGA 
GATGTGCGCA AATCTCAGCC AGTATTGATA ACAATAAATG GTGGCCCCGT GGTACGACTC 
AAAGGATCCA TAACTTCCAT GTCTTTTTTA GACTCATACG GTTCGATCAT ACCCTATACG 
TTTGAGACCT GGCGTGACGA GAAGAGGGAC AACAAAGATC GCACACCAAC AAAGAGCAGC 
AGCCGCACCA GTCCCACATT CACACCGACA ACTGCCAATA CATTATCAAA CACTTCGGTC 
GCGGGTGGTG CGTCCGCAGG CGGAGGAGCT GGCGGCGGTG GTGGTGCCGG CGGTGGTGCG 
GCCAGCGGTG GCGCAGCCGC AGGAGGGCCA GCCGGCGGTG GTGCCGGAGC AGGATCGGCG 
GCTGGAGCGG CCGGCGGCGT TGGAAGCGGT GTAACCAGCG GCGGGGGGAT GAGCAGCAGC 
AGCGCCAGCA GCAGCGGCAT CAGCGGAAGT GTGAGCACCG GCCTGGAAAC TCTCACCGAT 
CGACAATACA TCGTTATCGC AAGCGAGAAG CAAACAAAAG TCTTCGATGT CGCCAACCAG 
TGCTGCATCA ACCGCATACA ATTATCGGAA ATGGATTTTG CAGTCAAGGC AGAAACTATC 
ACGATGAAAG ATGGCTCTTG CTTAGCAACC TATCTTTCGA ATGGCCATCT AATGGTACAT 
AGTCTTCCTA GTCTAAAACT GTTATTGGAT ACTGATTTCT TACCACTTAT GGAACTAAGT 
TTTCAAACAA AATGTAAACA GGGAATTGTG GATCCAATGC TTTCGATATG GGGTCAACAG 
ATCATTGTTC ATGAAGATAC AACTCAAATA TCAAAAATAT TTTGCTTTAG TCATAAAGGT 
CATGGATTGT ACATGGCATC GCCCACCGAA ATTCAAAAAT TCACGATATC ATCGGAATTT 
TGTCAATTTA TTTTGGAGAT GATGGGTGAA CTATATACGG TACACGAAAT GCCCGAACAG 
CCGAAGGAGG GTTTCTTCAA GGGTCTATTT GGTGGTGGCG CCAAACTCCT GGATCGCGAG 
GAGCTATTTG GCGAGCAGAG CGGGAAACCG AATCGATCGG TGGCACGGCA CATACCCGGC 
CCCAATTTGG AGCAGCTCGG CCAGAGGGCT TCCACCGCCG CATCGGAGAT CAGCCGGGCG 
CACCAGCTGG CCATGGAGCG CGGCGAGAAG CTAAATTTGC TGGAGGAGCG CGCCGAGCGC 
ATGGCCAACA CTGCTCAAGA TTTCAGCGGC ACTGCGCATC AATTAATGCT TAAATATAAA 
GACAAGAAGT GGTATCAGTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727630.3
CDS	761..4924
Translation	MKKFTFKGVLDGFRQSVQPQATRQEQEIQEQLKADHFTLKKTFRHGFPYSPTSFAFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTVTADDTIHLWSIRQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYIINWNKAIEVVRTSHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWHYEGKYFVSSHTDGSICSWPTKPQAKPQSQVCPHAKINKDGNAEKCKPIYKVDLKSSATGETFTIFSGGMPSEKGSKSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLIDLLTPGFPCFESPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGEWSPASCSYSEIVITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPESDNPLAVHLIFLCSESRRLCVAGAMGQVMLFKFRKVESTSEVLVLEIPILYENFDDIYGTSPECDFLTGHQVQKAESSDSDKTAECSLKVRNGAQRKPPGFQSQLVCLTTGPNRRNVQVTSLCISSSYGLMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKRIENKEEQSRSPSSDQINGSGVATAAPQQQQQQQQQLQQSQQQQQQQLQQQQQQQQQQQQQQQQQQQSLGGDPGDHATSTSAGAATLAGSSIYGADDAGAGVASGGVGVGGISSAISGGFGGAAEPQQQQQVSQQLHQLQQLQQQSQQQQQSFEASCDPWGGECGGGGAGGSGGGGGASGGGGASGAGAQQHQYQHPHPYPHPHPYPHQTSVIQQQQSVTSYESLKKSKSQDKLDGTFSRSRSSSMSSIDMSSSESVTCLAFIESYAKRNDILTLVPTLWIGTSFGSILTLFITMPERDVRKSQPVLITINGGPVVRLKGSITSMSFLDSYGSIIPYTFETWRDEKRDNKDRTPTKSSSRTSPTFTPTTANTLSNTSVAGGASAGGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGGMSSSSASSSGISGSVSTGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETITMKDGSCLATYLSNGHLMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHEDTTQISKIFCFSHKGHGLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFGGGAKLLDREELFGEQSGKPNRSVARHIPGPNLEQLGQRASTAASEISRAHQLAMERGEKLNLLEERAERMANTAQDFSGTAHQLMLKYKDKKWYQL

Target ORF information:

RefSeq Version	NM_167330.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tomosyn, transcript variant L (Tomosyn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167330.3

ORF Insert Sequence:

ATGAAAAAGT TCACATTCAA AGGAGTTCTA GATGGATTTC GACAAAGCGT TCAGCCCCAA 
GCCACACGAC AGGAGCAGGA GATTCAGGAG CAACTCAAGG CCGATCACTT TACGTTAAAG 
AAAACCTTTC GCCATGGCTT TCCATACTCG CCCACATCCT TCGCCTTCGA TCCAGTACAA 
AAGCTACTAG CGATTGGCGA CAAATCGGGC TACATTCGCA TTTTGGGACG ACCTGGCGTC 
GATGCCCATG CCAAACACGA GGGCGAATCC GAGTGCGCCG TGCTCTTTGC CCAGTTCCTG 
GTCAACGAGG GCGCATTGGT CACCGTCACC GCCGATGACA CCATTCACTT GTGGAGCATT 
CGCCAAAAGA CACCGCGCAT TGTGCAGAGT CTGAAGTTTC AGAGGGAGCG CGTGACTTGC 
ATACACCTGC CTGTGGGCAG CAAATGGCTG TACGTGGGCA CCGAGAAGGG AAACATACAC 
GTTGTTCACA TAGATACATT TGCACTTAGT GGTTATATTA TTAACTGGAA TAAAGCAATT 
GAAGTGGTTA GAACTAGTCA TCCAGGTGCT GTGATTGCGT TATGTGATAA TCCATTGGAT 
GCAAACAAGT TGCTTATTGC ATTTGAGTGC GGGCTGCTTG TGCTGTGGGA TCTGAAGGCG 
AAATGCGCTG AGCTGCGATG GCAGGCGGCC GAGGCCGTCA AGTCCCTGGC CTGGCACTAC 
GAGGGCAAAT ATTTTGTGTC CTCGCACACG GATGGCTCCA TTTGCTCCTG GCCCACGAAG 
CCGCAGGCTA AGCCGCAGTC GCAGGTCTGT CCGCATGCTA AAATCAACAA GGATGGCAAT 
GCGGAGAAAT GCAAGCCGAT ATATAAAGTT GACTTGAAGT CCAGCGCAAC TGGGGAAACC 
TTCACAATTT TCTCGGGCGG CATGCCCTCG GAGAAGGGCT CCAAGTCCAA CTGCATCACG 
GTGATGGTGG GCAAGGCCAC CACGGTGCTG GAGATGGAGC ACGCCGTTTG CGATTTCATA 
ACACTCTGCG AGAATCCCTG GCCCTGCGAG ACCCAGGAGC CGTATGCTAT TGCCGTGCTG 
TTGCAATACG ACTTAGTATT AATAGACTTA TTAACACCCG GTTTTCCATG TTTTGAATCG 
CCTTATCCAA TGGACTTACA CGAATCACCT GTTACATGTT GCACATATTT AACCGATTGC 
CCATCGGATT TGGTTCCTGC TTTCTATTCA GTTGGTCGCA CAACGACAAG TAAAAAGAGC 
TGCTTCTCGG AGCGCGAATG GCCGATATCC GGTGGCGAGT GGTCGCCGGC GTCGTGCAGC 
TATTCGGAGA TCGTGATCAC CGGACACCAG GACGGCAGCC TCAAGTTCTG GGATTCCGGT 
GCCGGCACAC TGCAGATCCT CTATAAGCTG AAGACAGCCA AGATGTTCGA GAAGCCGCGC 
TCCCACGTCG CCCACTCGCA TCCGGAAAGC GACAATCCGC TGGCGGTGCA CCTAATCTTC 
CTTTGCTCGG AATCGCGACG CCTGTGTGTG GCCGGCGCCA TGGGACAGGT GATGCTCTTC 
AAGTTCCGCA AAGTGGAGTC CACGTCGGAG GTACTGGTGC TGGAGATACC CATACTCTAT 
GAGAATTTTG ATGACATTTA TGGGACAAGT CCCGAGTGCG ATTTTCTAAC GGGCCATCAA 
GTGCAGAAGG CTGAGTCGAG TGATTCGGAT AAGACGGCCG AGTGTTCGCT AAAGGTGCGC 
AATGGGGCGC AACGCAAGCC GCCGGGCTTC CAGTCGCAGC TGGTCTGCCT CACCACCGGA 
CCAAATCGCC GGAACGTGCA GGTCACCTCG CTGTGCATCA GCTCCAGTTA TGGCCTAATG 
GCTTATGGTA CCGAATATGG ACTTGTCATA ATTGATATAA TCCAAAAAAT TTGCCTATTG 
AGTGTTGCAT GCCCCGATCT GTATGGCGCA CATGATCCTT ACTCGCGAAC ACCAAAAAGT 
CCAAAACGTA TTGAAAACAA AGAGGAGCAA AGCCGATCGC CCAGCTCAGA TCAGATCAAC 
GGCAGCGGAG TTGCAACTGC AGCACCGCAG CAGCAACAGC AGCAGCAGCA ACAGTTGCAG 
CAATCACAGC AACAACAGCA GCAGCAGCTA CAGCAGCAAC AGCAGCAGCA GCAACAACAA 
CAGCAGCAGC AGCAACAACA ACAACAATCG TTAGGTGGTG ATCCTGGTGA TCATGCAACA 
TCGACGTCAG CGGGAGCAGC AACATTGGCG GGATCCTCCA TTTACGGTGC AGATGATGCA 
GGTGCGGGTG TTGCTAGTGG CGGTGTTGGT GTTGGTGGTA TTAGCAGTGC GATTAGCGGT 
GGATTTGGTG GTGCGGCTGA GCCGCAACAG CAGCAACAGG TGTCGCAACA GTTGCATCAA 
TTACAGCAAC TACAGCAGCA ATCGCAGCAG CAGCAGCAAT CGTTTGAGGC GTCTTGTGAT 
CCATGGGGCG GCGAGTGCGG CGGTGGTGGT GCTGGCGGCA GCGGCGGCGG CGGTGGTGCA 
AGCGGTGGTG GTGGTGCTAG CGGTGCAGGT GCGCAACAAC ATCAATATCA GCATCCGCAT 
CCGTATCCGC ATCCGCATCC GTATCCGCAT CAAACATCCG TTATACAGCA ACAGCAGTCA 
GTGACATCGT ACGAATCCCT AAAAAAGTCC AAATCACAAG ATAAATTGGA TGGAACTTTC 
TCAAGATCCA GATCATCGTC AATGTCCAGC ATTGATATGT CTTCCTCTGA ATCTGTTACC 
TGTTTAGCTT TTATCGAGAG TTATGCAAAA CGAAATGATA TTTTGACACT TGTTCCTACT 
TTGTGGATTG GAACTAGTTT CGGTTCGATA CTGACATTGT TCATCACCAT GCCCGAGCGA 
GATGTGCGCA AATCTCAGCC AGTATTGATA ACAATAAATG GTGGCCCCGT GGTACGACTC 
AAAGGATCCA TAACTTCCAT GTCTTTTTTA GACTCATACG GTTCGATCAT ACCCTATACG 
TTTGAGACCT GGCGTGACGA GAAGAGGGAC AACAAAGATC GCACACCAAC AAAGAGCAGC 
AGCCGCACCA GTCCCACATT CACACCGACA ACTGCCAATA CATTATCAAA CACTTCGGTC 
GCGGGTGGTG CGTCCGCAGG CGGAGGAGCT GGCGGCGGTG GTGGTGCCGG CGGTGGTGCG 
GCCAGCGGTG GCGCAGCCGC AGGAGGGCCA GCCGGCGGTG GTGCCGGAGC AGGATCGGCG 
GCTGGAGCGG CCGGCGGCGT TGGAAGCGGT GTAACCAGCG GCGGGGGGAT GAGCAGCAGC 
AGCGCCAGCA GCAGCGGCAT CAGCGGAAGT GTGAGCACCG GCCTGGAAAC TCTCACCGAT 
CGACAATACA TCGTTATCGC AAGCGAGAAG CAAACAAAAG TCTTCGATGT CGCCAACCAG 
TGCTGCATCA ACCGCATACA ATTATCGGAA ATGGATTTTG CAGTCAAGGC AGAAACTATC 
ACGATGAAAG ATGGCTCTTG CTTAGCAACC TATCTTTCGA ATGGCCATCT AATGGTACAT 
AGTCTTCCTA GTCTAAAACT GTTATTGGAT ACTGATTTCT TACCACTTAT GGAACTAAGT 
TTTCAAACAA AATGTAAACA GGGAATTGTG GATCCAATGC TTTCGATATG GGGTCAACAG 
ATCATTGTTC ATGAAGATAC AACTCAAATA TCAAAAATAT TTTGCTTTAG TCATAAAGGT 
CATGGATTGT ACATGGCATC GCCCACCGAA ATTCAAAAAT TCACGATATC ATCGGAATTT 
TGTCAATTTA TTTTGGAGAT GATGGGTGAA CTATATACGG TACACGAAAT GCCCGAACAG 
CCGAAGGAGG GTTTCTTCAA GGGTCTATTT GGTGGTGGCG CCAAACTCCT GGATCGCGAG 
GAGCTATTTG GCGAGCAGAG CGGGAAACCG AATCGATCGG TGGCACGGCA CATACCCGGC 
CCCAATTTGG AGCAGCTCGG CCAGAGGGCT TCCACCGCCG CATCGGAGAT CAGCCGGGCG 
CACCAGCTGG CCATGGAGCG CGGCGAGAAG CTAAATTTGC TGGAGGAGCG CGCCGAGCGC 
ATGGCCAACA CTGCTCAAGA TTTCAGCGGC ACTGCGCATC AATTAATGCT TAAATATAAA 
GACAAGAAGT GGTATCAGTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tomosyn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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