LIMK1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following LIMK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa01405 NM_078584.3
Latest version!
Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa01406 NM_167327.2
Latest version!
Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa01407 NM_001169262.1
Latest version!
Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa01407 NM_167326.2
Latest version!
Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa02798 NM_001169263.1
Latest version!
Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa01405
    Clone ID Related Accession (Same CDS sequence) NM_078584.3
    Accession Version NM_078584.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product LIM domain kinase 1, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_078584.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCATCATC AGCAGCGCCT GCGGGCAAAT GGCGGACGAG GAGGCACTGG ATTGGGCGCA 
    GGATCAGGAC CTGTGTCCGG AGGACACTCA CCATTGTGCG CCCATTGTCG GGGTCAACTA
    CTGCCACATC CAGAGGAGCC AATCGTGATG GCTCTCGGCC AGCAATGGCA CTGCGATTGC
    TTTCGCTGCT CCGTTTGCGA GGGCCATCTG CATAACTGGT ACTTCGAACG GGAGGGCTTA
    CTGTACTGTC GTGAGGATTA CTACGGCCGA TTCGGCGATG CCTGTCAGCA GTGTATGGCC
    GTTATCACCG GACCCGTAAT GGTCGCCGGC GAGCACAAAT TCCATCCGGA GTGCTTCTGC
    TGCACGGCAT GCGGCTCCTT CATCGGCGAG GGCGAATCGT ACGCCCTTGT CGAGCGTTCG
    AAGCTCTATT GTGGCCAGTG CTATGGCAAA CGTTCCTGCC AGCCGGCGGA TGCGAAGGCG
    CGGATCACAA CGGCCGGCAA GCCGATGCAC TCCATCCGTC TGGTCGAGAT ACCCAAGGAC
    GCCACGCCCG GCCTGAGGGT CGACGGAGTG GCCCTCGACG ATGGTTGTCC CACGGTCCGC
    ATTACAGACT TGTTCTGTAA CTTCTTCTTG TGGCTAACCT CGATGGCGGA ACAGTGCTGT
    GGGGCGCCTT ATAGAATTGA TGTGAATCTT ACAAATCTGC ATATTGGAGA TAGGATATTG
    GAGGTGAACG GCACACCAGT TAGTGATTCG TCCGTGGAAC AGATCGACAA ATTGATACGG
    AGTAATGAGA AGATGCTTCA GCTGACCGTG GAGCATGATC CGGTGCAAGT GTGCCGCTCC
    TGCAGCCAAG CGGACATCCA GCGGGCCATG TCAGCGTCCA CGTTAATCCT GCCGCTCAGC
    ACATCGGCCT CCAGTGTCGA GGTGGGCAGG GAGCGTCTGT ACAAGACACC CGGCGAACAG
    GGCACGAAGG CCAGGAAACT GCGACAAGCC ACCAATGCCT CTACGACAAT ACCACCGGCA
    GCTGGAGCCA CCGCAATGAC GCAACTAAAG GAGAAGGAGC GTTGTTCCAG TCTGTCCAAG
    TTGCTGGATG AACAGCATCA GGCACAACAG CATTCGGCGC ATCCTCAGCT CTATGATCTG
    TCCAGGACGC AATCCTGCCG TGTCGTCCAA AAGCCACAAC GAATCTTTCG TGCAACTGAT
    CTGGTTATAG GAGAGAAGCT AGGCGAGGGC TTCTTTGGCA AGGTTTTCAA GGTGACCCAC
    CGTCAGAGCG GCGAGGTGAT GGTCCTCAAA GAACTGCATC GTGCGGATGA GGAAGCGCAA
    AGGAATTTCA TCAAGGAAGT GGCCGTTCTC CGTTTGCTGG ACCATCGGCA TGTGCTCAAA
    TTCATTGGTG TTCTGTACAA GGATAAGAAA CTGCACATGG TCACGGAGTA CGTGGCCGGT
    GGTTGTCTCA AGGAGCTGAT CCACGATCCA GCCCAAGTAC TCCCTTGGCC CCAGCGCGTC
    CGCTTGGCCA GGGATATTGC GTGCGGCATG AGCTATCTGC ACTCGATGAA CATCATCCAT
    CGCGATTTGA ACTCAATGAA TTGCCTGGTC CGTGAAGATC GCTCGGTCAT AGTGGCGGAT
    TTTGGACTAG CTCGCAGTGT GGATGCGCCG CGGCTGCCAA GTGGCAATAT GACGCCAGGC
    GGCTATGGAT CAGGCGCTAA CAGCGATGCG CCCATGTCAC CGAGTGGAAC ACTAAGGCGC
    AGCAAGAGCA GGCAGCGAAG GCAACGTTAC ACGGTGGTCG GGAATCCCTA CTGGATGGCA
    CCCGAGATGA TGAAAGGTCT CAAATACGAC GAAAAGGTGG ATGTCTTCTC CTTTGGCATT
    ATGTTGTGTG AGATCATTGG ACGCGTGGAA GCCGATCCGG ATTTTATGCC TCGCAATTCG
    GACTTCAGTC TCAATCAGCA GGAGTTTCGC GAGAAGTTCT GCGCTCAGTG TCCAGAGCCT
    TTCGTTAAAG TGGCCTTCGT GTGCTGTGAT CTTAATCCCG ATATGCGTCC GTGTTTCGAA
    ACTCTGCACG TTTGGCTGCA GCGTCTCGCC GATGATCTGG CCGCCGATCG TGTGCCGCCA
    GAGCGTCTGC TGCACGAGAT TGAGACCTTT CAGGAATGGT ACGCCAGCTC TGAGGACGCC
    TTGTCACCCA CCTCCCAGCG CAGCTTAAAT AATCTCGATG AGTTGGTCAA GAGTGCGGTG
    GATTCGGAAA TTTCGCCGGT AGAAAAAGAG AAGGAGAATA TGGTGATTAA GCCCCAGGAT
    ATACCGAAAT CTCCGCATCT CGGCAAGGAT TTCTCGCCCA GCGGCGAGAG ACTGCGTGAC
    AGTATGCGGG CCAGGCGTCG CCAACGTTTC TTGGGCGCCC AGGAGGAGCG TCGTAATCTC
    ACGCCCGACA CGGAGTCCAA GGAAAGGGCT TTGAAGAAGG CGCTGAAGAA ATGTCGTCCG
    TTTGGCGAAA GAGGTTACCT GGTGGATCTG CGTGCCGGTG CCGAATTGCA GCTAGAGGAT
    GTCAGGGATT TGAACACCTA TTCCGATGTG GACTCCAGTT GCGATACTAG CCTAAATTAC
    CATGATGTAA ATAATCTGCC AGCAGCGCAA GAGGATGAGA ATACAGTGAA ACCGGGCAAG
    GAAGAACTGT TGGAGGAGAG CACAAATAAA CCCAGCAACC AGGAGTCACA ACACCATCGT
    CTGGCCATCG ATGATATGCG CACCCGCCTG AATCAGTGCC GCAGCAAATT CGAGCATTTG
    GAGGAGGCTA GTCGGCGGAA CTTCAACCAG TCGCAGCACT CCATGAAGAA CTTTTTCAAA
    ACCCCACCGG TGGCCCTTAA GATGTTTCAG CGCCTGGAGC ACGAGGCTGC AGCCCTGAAT
    GGTGGCAACA ATTGCCCACC GCCACCACCA CGTACGCAGC GCATCAATCA GACGCCCATT
    TTCGGTCGAA AGAATCCGCC AGTGGCAATC GTCGGGCAAA AACTACAGCA TGCTGAGTCC
    CTAGAGGACC TGGCCTCCAG CGGAGTTGCC AAACAGCTGG CCACACCAGC ACCCAAACGC
    AGCAAAGCCA CAGCAACGAC CAAAGGCGGC CAGAGCAGCA ATCCGCCACT TTTCCTGCCG
    CCTAGCCTTA ACATAAGTGT CGCATTAAAT AGCAATGGAA ATGTGACCAC AACGACCAAT
    ACCAATAGTA GTTGTCCACC ATCCGCATCA GACTGGCTGC CGAAGAAGCA CAAGCTAACT
    TTGCCACTTC CGTCGGCCCA ACAGCAGCGA ACGAGCAGCA ATCATCGCCT GCCAATGTGC
    AATAACAAAG GGAAGACACT GAAGCCACTG CCAAGTCGCA CGGGCAGTCA AGGCATTCCC
    GCAAGCAATT GCGTCTCGCC GACGAGGAGC AGCAGGCCCG GATCACCCAC CAAGCATCTG
    GCCCAGAGGC ACACAGCCGC CACGGCGCAG CGACTGACAA ATGCGGCCGC CACTCACCAG
    CAGCAGCATC AGCAACAATC CTCGAAGACG ACGCGCCTGA ATATCCTGAG TCCGGAAAAG
    GTGCATCGCC TGGGCGCTCG ACTCACCGAT CAGAAGCAGA AAATGCGCGA GGAGGCCGCA
    GCGACGGCCT CGTCCGTCGG AGGAGCCGGT TGTGCGGCAG GAACAGCGGC TGGAAGCTTA
    AATGGTCATC GGACCATCGG ATCCTCCGGC ACACCGAATT CGGCGGTCGG TGAACGGAGG
    AGGCGAGCAG CTCCATCGCC GCCAGTTCGC ACGCATTTCA ATACGCGCTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_511139.2
    CDS300..4073
    Translation

    Target ORF information:

    RefSeq Version NM_078584.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_078584.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCATCATC AGCAGCGCCT GCGGGCAAAT GGCGGACGAG GAGGCACTGG ATTGGGCGCA 
    GGATCAGGAC CTGTGTCCGG AGGACACTCA CCATTGTGCG CCCATTGTCG GGGTCAACTA
    CTGCCACATC CAGAGGAGCC AATCGTGATG GCTCTCGGCC AGCAATGGCA CTGCGATTGC
    TTTCGCTGCT CCGTTTGCGA GGGCCATCTG CATAACTGGT ACTTCGAACG GGAGGGCTTA
    CTGTACTGTC GTGAGGATTA CTACGGCCGA TTCGGCGATG CCTGTCAGCA GTGTATGGCC
    GTTATCACCG GACCCGTAAT GGTCGCCGGC GAGCACAAAT TCCATCCGGA GTGCTTCTGC
    TGCACGGCAT GCGGCTCCTT CATCGGCGAG GGCGAATCGT ACGCCCTTGT CGAGCGTTCG
    AAGCTCTATT GTGGCCAGTG CTATGGCAAA CGTTCCTGCC AGCCGGCGGA TGCGAAGGCG
    CGGATCACAA CGGCCGGCAA GCCGATGCAC TCCATCCGTC TGGTCGAGAT ACCCAAGGAC
    GCCACGCCCG GCCTGAGGGT CGACGGAGTG GCCCTCGACG ATGGTTGTCC CACGGTCCGC
    ATTACAGACT TGTTCTGTAA CTTCTTCTTG TGGCTAACCT CGATGGCGGA ACAGTGCTGT
    GGGGCGCCTT ATAGAATTGA TGTGAATCTT ACAAATCTGC ATATTGGAGA TAGGATATTG
    GAGGTGAACG GCACACCAGT TAGTGATTCG TCCGTGGAAC AGATCGACAA ATTGATACGG
    AGTAATGAGA AGATGCTTCA GCTGACCGTG GAGCATGATC CGGTGCAAGT GTGCCGCTCC
    TGCAGCCAAG CGGACATCCA GCGGGCCATG TCAGCGTCCA CGTTAATCCT GCCGCTCAGC
    ACATCGGCCT CCAGTGTCGA GGTGGGCAGG GAGCGTCTGT ACAAGACACC CGGCGAACAG
    GGCACGAAGG CCAGGAAACT GCGACAAGCC ACCAATGCCT CTACGACAAT ACCACCGGCA
    GCTGGAGCCA CCGCAATGAC GCAACTAAAG GAGAAGGAGC GTTGTTCCAG TCTGTCCAAG
    TTGCTGGATG AACAGCATCA GGCACAACAG CATTCGGCGC ATCCTCAGCT CTATGATCTG
    TCCAGGACGC AATCCTGCCG TGTCGTCCAA AAGCCACAAC GAATCTTTCG TGCAACTGAT
    CTGGTTATAG GAGAGAAGCT AGGCGAGGGC TTCTTTGGCA AGGTTTTCAA GGTGACCCAC
    CGTCAGAGCG GCGAGGTGAT GGTCCTCAAA GAACTGCATC GTGCGGATGA GGAAGCGCAA
    AGGAATTTCA TCAAGGAAGT GGCCGTTCTC CGTTTGCTGG ACCATCGGCA TGTGCTCAAA
    TTCATTGGTG TTCTGTACAA GGATAAGAAA CTGCACATGG TCACGGAGTA CGTGGCCGGT
    GGTTGTCTCA AGGAGCTGAT CCACGATCCA GCCCAAGTAC TCCCTTGGCC CCAGCGCGTC
    CGCTTGGCCA GGGATATTGC GTGCGGCATG AGCTATCTGC ACTCGATGAA CATCATCCAT
    CGCGATTTGA ACTCAATGAA TTGCCTGGTC CGTGAAGATC GCTCGGTCAT AGTGGCGGAT
    TTTGGACTAG CTCGCAGTGT GGATGCGCCG CGGCTGCCAA GTGGCAATAT GACGCCAGGC
    GGCTATGGAT CAGGCGCTAA CAGCGATGCG CCCATGTCAC CGAGTGGAAC ACTAAGGCGC
    AGCAAGAGCA GGCAGCGAAG GCAACGTTAC ACGGTGGTCG GGAATCCCTA CTGGATGGCA
    CCCGAGATGA TGAAAGGTCT CAAATACGAC GAAAAGGTGG ATGTCTTCTC CTTTGGCATT
    ATGTTGTGTG AGATCATTGG ACGCGTGGAA GCCGATCCGG ATTTTATGCC TCGCAATTCG
    GACTTCAGTC TCAATCAGCA GGAGTTTCGC GAGAAGTTCT GCGCTCAGTG TCCAGAGCCT
    TTCGTTAAAG TGGCCTTCGT GTGCTGTGAT CTTAATCCCG ATATGCGTCC GTGTTTCGAA
    ACTCTGCACG TTTGGCTGCA GCGTCTCGCC GATGATCTGG CCGCCGATCG TGTGCCGCCA
    GAGCGTCTGC TGCACGAGAT TGAGACCTTT CAGGAATGGT ACGCCAGCTC TGAGGACGCC
    TTGTCACCCA CCTCCCAGCG CAGCTTAAAT AATCTCGATG AGTTGGTCAA GAGTGCGGTG
    GATTCGGAAA TTTCGCCGGT AGAAAAAGAG AAGGAGAATA TGGTGATTAA GCCCCAGGAT
    ATACCGAAAT CTCCGCATCT CGGCAAGGAT TTCTCGCCCA GCGGCGAGAG ACTGCGTGAC
    AGTATGCGGG CCAGGCGTCG CCAACGTTTC TTGGGCGCCC AGGAGGAGCG TCGTAATCTC
    ACGCCCGACA CGGAGTCCAA GGAAAGGGCT TTGAAGAAGG CGCTGAAGAA ATGTCGTCCG
    TTTGGCGAAA GAGGTTACCT GGTGGATCTG CGTGCCGGTG CCGAATTGCA GCTAGAGGAT
    GTCAGGGATT TGAACACCTA TTCCGATGTG GACTCCAGTT GCGATACTAG CCTAAATTAC
    CATGATGTAA ATAATCTGCC AGCAGCGCAA GAGGATGAGA ATACAGTGAA ACCGGGCAAG
    GAAGAACTGT TGGAGGAGAG CACAAATAAA CCCAGCAACC AGGAGTCACA ACACCATCGT
    CTGGCCATCG ATGATATGCG CACCCGCCTG AATCAGTGCC GCAGCAAATT CGAGCATTTG
    GAGGAGGCTA GTCGGCGGAA CTTCAACCAG TCGCAGCACT CCATGAAGAA CTTTTTCAAA
    ACCCCACCGG TGGCCCTTAA GATGTTTCAG CGCCTGGAGC ACGAGGCTGC AGCCCTGAAT
    GGTGGCAACA ATTGCCCACC GCCACCACCA CGTACGCAGC GCATCAATCA GACGCCCATT
    TTCGGTCGAA AGAATCCGCC AGTGGCAATC GTCGGGCAAA AACTACAGCA TGCTGAGTCC
    CTAGAGGACC TGGCCTCCAG CGGAGTTGCC AAACAGCTGG CCACACCAGC ACCCAAACGC
    AGCAAAGCCA CAGCAACGAC CAAAGGCGGC CAGAGCAGCA ATCCGCCACT TTTCCTGCCG
    CCTAGCCTTA ACATAAGTGT CGCATTAAAT AGCAATGGAA ATGTGACCAC AACGACCAAT
    ACCAATAGTA GTTGTCCACC ATCCGCATCA GACTGGCTGC CGAAGAAGCA CAAGCTAACT
    TTGCCACTTC CGTCGGCCCA ACAGCAGCGA ACGAGCAGCA ATCATCGCCT GCCAATGTGC
    AATAACAAAG GGAAGACACT GAAGCCACTG CCAAGTCGCA CGGGCAGTCA AGGCATTCCC
    GCAAGCAATT GCGTCTCGCC GACGAGGAGC AGCAGGCCCG GATCACCCAC CAAGCATCTG
    GCCCAGAGGC ACACAGCCGC CACGGCGCAG CGACTGACAA ATGCGGCCGC CACTCACCAG
    CAGCAGCATC AGCAACAATC CTCGAAGACG ACGCGCCTGA ATATCCTGAG TCCGGAAAAG
    GTGCATCGCC TGGGCGCTCG ACTCACCGAT CAGAAGCAGA AAATGCGCGA GGAGGCCGCA
    GCGACGGCCT CGTCCGTCGG AGGAGCCGGT TGTGCGGCAG GAACAGCGGC TGGAAGCTTA
    AATGGTCATC GGACCATCGG ATCCTCCGGC ACACCGAATT CGGCGGTCGG TGAACGGAGG
    AGGCGAGCAG CTCCATCGCC GCCAGTTCGC ACGCATTTCA ATACGCGCTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa01406
    Clone ID Related Accession (Same CDS sequence) NM_167327.2
    Accession Version NM_167327.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product LIM domain kinase 1, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_167327.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTCTTTTT GGTTACATTT TGTACTGAAA AAAATTTTAC CATGGCTCAA ATCTACGAAA 
    ATTGATGTGA ATCTTACAAA TCTGCATATT GGAGATAGGA TATTGGAGGT GAACGGCACA
    CCAGTTAGTG ATTCGTCCGT GGAACAGATC GACAAATTGA TACGGAGTAA TGAGAAGATG
    CTTCAGCTGA CCGTGGAGCA TGATCCGGTG CAAGTGTGCC GCTCCTGCAG CCAAGCGGAC
    ATCCAGCGGG CCATGTCAGC GTCCACGTTA ATCCTGCCGC TCAGCACATC GGCCTCCAGT
    GTCGAGGTGG GCAGGGAGCG TCTGTACAAG ACACCCGGCG AACAGGGCAC GAAGGCCAGG
    AAACTGCGAC AAGCCACCAA TGCCTCTACG ACAATACCAC CGGCAGCTGG AGCCACCGCA
    ATGACGCAAC TAAAGGAGAA GGAGCGTTGT TCCAGTCTGT CCAAGTTGCT GGATGAACAG
    CATCAGGCAC AACAGCATTC GGCGCATCCT CAGCTCTATG ATCTGTCCAG GACGCAATCC
    TGCCGTGTCG TCCAAAAGCC ACAACGAATC TTTCGTGCAA CTGATCTGGT TATAGGAGAG
    AAGCTAGGCG AGGGCTTCTT TGGCAAGGTT TTCAAGGTGA CCCACCGTCA GAGCGGCGAG
    GTGATGGTCC TCAAAGAACT GCATCGTGCG GATGAGGAAG CGCAAAGGAA TTTCATCAAG
    GAAGTGGCCG TTCTCCGTTT GCTGGACCAT CGGCATGTGC TCAAATTCAT TGGTGTTCTG
    TACAAGGATA AGAAACTGCA CATGGTCACG GAGTACGTGG CCGGTGGTTG TCTCAAGGAG
    CTGATCCACG ATCCAGCCCA AGTACTCCCT TGGCCCCAGC GCGTCCGCTT GGCCAGGGAT
    ATTGCGTGCG GCATGAGCTA TCTGCACTCG ATGAACATCA TCCATCGCGA TTTGAACTCA
    ATGAATTGCC TGGTCCGTGA AGATCGCTCG GTCATAGTGG CGGATTTTGG ACTAGCTCGC
    AGTGTGGATG CGCCGCGGCT GCCAAGTGGC AATATGACGC CAGGCGGCTA TGGATCAGGC
    GCTAACAGCG ATGCGCCCAT GTCACCGAGT GGAACACTAA GGCGCAGCAA GAGCAGGCAG
    CGAAGGCAAC GTTACACGGT GGTCGGGAAT CCCTACTGGA TGGCACCCGA GATGATGAAA
    GGTCTCAAAT ACGACGAAAA GGTGGATGTC TTCTCCTTTG GCATTATGTT GTGTGAGATC
    ATTGGACGCG TGGAAGCCGA TCCGGATTTT ATGCCTCGCA ATTCGGACTT CAGTCTCAAT
    CAGCAGGAGT TTCGCGAGAA GTTCTGCGCT CAGTGTCCAG AGCCTTTCGT TAAAGTGGCC
    TTCGTGTGCT GTGATCTTAA TCCCGATATG CGTCCGTGTT TCGAAACTCT GCACGTTTGG
    CTGCAGCGTC TCGCCGATGA TCTGGCCGCC GATCGTGTGC CGCCAGAGCG TCTGCTGCAC
    GAGATTGAGA CCTTTCAGGA ATGGTACGCC AGCTCTGAGG ACGCCTTGTC ACCCACCTCC
    CAGCGCAGCT TAAATAATCT CGATGAGTTG GTCAAGAGTG CGGTGGATTC GGAAATTTCG
    CCGGTAGAAA AAGAGAAGGA GAATATGGTG ATTAAGCCCC AGGATATACC GAAATCTCCG
    CATCTCGGCA AGGATTTCTC GCCCAGCGGC GAGAGACTGC GTGACAGTAT GCGGGCCAGG
    CGTCGCCAAC GTTTCTTGGG CGCCCAGGAG GAGCGTCGTA ATCTCACGCC CGACACGGAG
    TCCAAGGAAA GGGCTTTGAA GAAGGCGCTG AAGAAATGTC GTCCGTTTGG CGAAAGAGGT
    TACCTGGTGG ATCTGCGTGC CGGTGCCGAA TTGCAGCTAG AGGATGTCAG GGATTTGAAC
    ACCTATTCCG ATGTGGACTC CAGTTGCGAT ACTAGCCTAA ATTACCATGA TGTAAATAAT
    CTGCCAGCAG CGCAAGAGGA TGAGAATACA GTGAAACCGG GCAAGGAAGA ACTGTTGGAG
    GAGAGCACAA ATAAACCCAG CAACCAGGAG TCACAACACC ATCGTCTGGC CATCGATGAT
    ATGCGCACCC GCCTGAATCA GTGCCGCAGC AAATTCGAGC ATTTGGAGGA GGCTAGTCGG
    CGGAACTTCA ACCAGTCGCA GCACTCCATG AAGAACTTTT TCAAAACCCC ACCGGTGGCC
    CTTAAGATGT TTCAGCGCCT GGAGCACGAG GCTGCAGCCC TGAATGGTGG CAACAATTGC
    CCACCGCCAC CACCACGTAC GCAGCGCATC AATCAGACGC CCATTTTCGG TCGAAAGAAT
    CCGCCAGTGG CAATCGTCGG GCAAAAACTA CAGCATGCTG AGTCCCTAGA GGACCTGGCC
    TCCAGCGGAG TTGCCAAACA GCTGGCCACA CCAGCACCCA AACGCAGCAA AGCCACAGCA
    ACGACCAAAG GCGGCCAGAG CAGCAATCCG CCACTTTTCC TGCCGCCTAG CCTTAACATA
    AGTGTCGCAT TAAATAGCAA TGGAAATGTG ACCACAACGA CCAATACCAA TAGTAGTTGT
    CCACCATCCG CATCAGACTG GCTGCCGAAG AAGCACAAGC TAACTTTGCC ACTTCCGTCG
    GCCCAACAGC AGCGAACGAG CAGCAATCAT CGCCTGCCAA TGTGCAATAA CAAAGGGAAG
    ACACTGAAGC CACTGCCAAG TCGCACGGGC AGTCAAGGCA TTCCCGCAAG CAATTGCGTC
    TCGCCGACGA GGAGCAGCAG GCCCGGATCA CCCACCAAGC ATCTGGCCCA GAGGCACACA
    GCCGCCACGG CGCAGCGACT GACAAATGCG GCCGCCACTC ACCAGCAGCA GCATCAGCAA
    CAATCCTCGA AGACGACGCG CCTGAATATC CTGAGTCCGG AAAAGGTGCA TCGCCTGGGC
    GCTCGACTCA CCGATCAGAA GCAGAAAATG CGCGAGGAGG CCGCAGCGAC GGCCTCGTCC
    GTCGGAGGAG CCGGTTGTGC GGCAGGAACA GCGGCTGGAA GCTTAAATGG TCATCGGACC
    ATCGGATCCT CCGGCACACC GAATTCGGCG GTCGGTGAAC GGAGGAGGCG AGCAGCTCCA
    TCGCCGCCAG TTCGCACGCA TTTCAATACG CGCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_727622.1
    CDS102..3260
    Translation

    Target ORF information:

    RefSeq Version NM_167327.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_167327.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTCTTTTT GGTTACATTT TGTACTGAAA AAAATTTTAC CATGGCTCAA ATCTACGAAA 
    ATTGATGTGA ATCTTACAAA TCTGCATATT GGAGATAGGA TATTGGAGGT GAACGGCACA
    CCAGTTAGTG ATTCGTCCGT GGAACAGATC GACAAATTGA TACGGAGTAA TGAGAAGATG
    CTTCAGCTGA CCGTGGAGCA TGATCCGGTG CAAGTGTGCC GCTCCTGCAG CCAAGCGGAC
    ATCCAGCGGG CCATGTCAGC GTCCACGTTA ATCCTGCCGC TCAGCACATC GGCCTCCAGT
    GTCGAGGTGG GCAGGGAGCG TCTGTACAAG ACACCCGGCG AACAGGGCAC GAAGGCCAGG
    AAACTGCGAC AAGCCACCAA TGCCTCTACG ACAATACCAC CGGCAGCTGG AGCCACCGCA
    ATGACGCAAC TAAAGGAGAA GGAGCGTTGT TCCAGTCTGT CCAAGTTGCT GGATGAACAG
    CATCAGGCAC AACAGCATTC GGCGCATCCT CAGCTCTATG ATCTGTCCAG GACGCAATCC
    TGCCGTGTCG TCCAAAAGCC ACAACGAATC TTTCGTGCAA CTGATCTGGT TATAGGAGAG
    AAGCTAGGCG AGGGCTTCTT TGGCAAGGTT TTCAAGGTGA CCCACCGTCA GAGCGGCGAG
    GTGATGGTCC TCAAAGAACT GCATCGTGCG GATGAGGAAG CGCAAAGGAA TTTCATCAAG
    GAAGTGGCCG TTCTCCGTTT GCTGGACCAT CGGCATGTGC TCAAATTCAT TGGTGTTCTG
    TACAAGGATA AGAAACTGCA CATGGTCACG GAGTACGTGG CCGGTGGTTG TCTCAAGGAG
    CTGATCCACG ATCCAGCCCA AGTACTCCCT TGGCCCCAGC GCGTCCGCTT GGCCAGGGAT
    ATTGCGTGCG GCATGAGCTA TCTGCACTCG ATGAACATCA TCCATCGCGA TTTGAACTCA
    ATGAATTGCC TGGTCCGTGA AGATCGCTCG GTCATAGTGG CGGATTTTGG ACTAGCTCGC
    AGTGTGGATG CGCCGCGGCT GCCAAGTGGC AATATGACGC CAGGCGGCTA TGGATCAGGC
    GCTAACAGCG ATGCGCCCAT GTCACCGAGT GGAACACTAA GGCGCAGCAA GAGCAGGCAG
    CGAAGGCAAC GTTACACGGT GGTCGGGAAT CCCTACTGGA TGGCACCCGA GATGATGAAA
    GGTCTCAAAT ACGACGAAAA GGTGGATGTC TTCTCCTTTG GCATTATGTT GTGTGAGATC
    ATTGGACGCG TGGAAGCCGA TCCGGATTTT ATGCCTCGCA ATTCGGACTT CAGTCTCAAT
    CAGCAGGAGT TTCGCGAGAA GTTCTGCGCT CAGTGTCCAG AGCCTTTCGT TAAAGTGGCC
    TTCGTGTGCT GTGATCTTAA TCCCGATATG CGTCCGTGTT TCGAAACTCT GCACGTTTGG
    CTGCAGCGTC TCGCCGATGA TCTGGCCGCC GATCGTGTGC CGCCAGAGCG TCTGCTGCAC
    GAGATTGAGA CCTTTCAGGA ATGGTACGCC AGCTCTGAGG ACGCCTTGTC ACCCACCTCC
    CAGCGCAGCT TAAATAATCT CGATGAGTTG GTCAAGAGTG CGGTGGATTC GGAAATTTCG
    CCGGTAGAAA AAGAGAAGGA GAATATGGTG ATTAAGCCCC AGGATATACC GAAATCTCCG
    CATCTCGGCA AGGATTTCTC GCCCAGCGGC GAGAGACTGC GTGACAGTAT GCGGGCCAGG
    CGTCGCCAAC GTTTCTTGGG CGCCCAGGAG GAGCGTCGTA ATCTCACGCC CGACACGGAG
    TCCAAGGAAA GGGCTTTGAA GAAGGCGCTG AAGAAATGTC GTCCGTTTGG CGAAAGAGGT
    TACCTGGTGG ATCTGCGTGC CGGTGCCGAA TTGCAGCTAG AGGATGTCAG GGATTTGAAC
    ACCTATTCCG ATGTGGACTC CAGTTGCGAT ACTAGCCTAA ATTACCATGA TGTAAATAAT
    CTGCCAGCAG CGCAAGAGGA TGAGAATACA GTGAAACCGG GCAAGGAAGA ACTGTTGGAG
    GAGAGCACAA ATAAACCCAG CAACCAGGAG TCACAACACC ATCGTCTGGC CATCGATGAT
    ATGCGCACCC GCCTGAATCA GTGCCGCAGC AAATTCGAGC ATTTGGAGGA GGCTAGTCGG
    CGGAACTTCA ACCAGTCGCA GCACTCCATG AAGAACTTTT TCAAAACCCC ACCGGTGGCC
    CTTAAGATGT TTCAGCGCCT GGAGCACGAG GCTGCAGCCC TGAATGGTGG CAACAATTGC
    CCACCGCCAC CACCACGTAC GCAGCGCATC AATCAGACGC CCATTTTCGG TCGAAAGAAT
    CCGCCAGTGG CAATCGTCGG GCAAAAACTA CAGCATGCTG AGTCCCTAGA GGACCTGGCC
    TCCAGCGGAG TTGCCAAACA GCTGGCCACA CCAGCACCCA AACGCAGCAA AGCCACAGCA
    ACGACCAAAG GCGGCCAGAG CAGCAATCCG CCACTTTTCC TGCCGCCTAG CCTTAACATA
    AGTGTCGCAT TAAATAGCAA TGGAAATGTG ACCACAACGA CCAATACCAA TAGTAGTTGT
    CCACCATCCG CATCAGACTG GCTGCCGAAG AAGCACAAGC TAACTTTGCC ACTTCCGTCG
    GCCCAACAGC AGCGAACGAG CAGCAATCAT CGCCTGCCAA TGTGCAATAA CAAAGGGAAG
    ACACTGAAGC CACTGCCAAG TCGCACGGGC AGTCAAGGCA TTCCCGCAAG CAATTGCGTC
    TCGCCGACGA GGAGCAGCAG GCCCGGATCA CCCACCAAGC ATCTGGCCCA GAGGCACACA
    GCCGCCACGG CGCAGCGACT GACAAATGCG GCCGCCACTC ACCAGCAGCA GCATCAGCAA
    CAATCCTCGA AGACGACGCG CCTGAATATC CTGAGTCCGG AAAAGGTGCA TCGCCTGGGC
    GCTCGACTCA CCGATCAGAA GCAGAAAATG CGCGAGGAGG CCGCAGCGAC GGCCTCGTCC
    GTCGGAGGAG CCGGTTGTGC GGCAGGAACA GCGGCTGGAA GCTTAAATGG TCATCGGACC
    ATCGGATCCT CCGGCACACC GAATTCGGCG GTCGGTGAAC GGAGGAGGCG AGCAGCTCCA
    TCGCCGCCAG TTCGCACGCA TTTCAATACG CGCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa01407
    Clone ID Related Accession (Same CDS sequence) NM_001169262.1 , NM_167326.2
    Accession Version NM_001169262.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product LIM domain kinase 1, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCATCATC AGCAGCGCCT GCGGGCAAAT GGCGGACGAG GAGGCACTGG ATTGGGCGCA 
    GGATCAGGAC CTGTGTCCGG AGGACACTCA CCATTGTGCG CCCATTGTCG GGGTCAACTA
    CTGCCACATC CAGAGGAGCC AATCGTGATG GCTCTCGGCC AGCAATGGCA CTGCGATTGC
    TTTCGCTGCT CCGTTTGCGA GGGCCATCTG CATAACTGGT ACTTCGAACG GGAGGGCTTA
    CTGTACTGTC GTGAGGATTA CTACGGCCGA TTCGGCGATG CCTGTCAGCA GTGTATGGCC
    GTTATCACCG GACCCGTAAT GGTCGCCGGC GAGCACAAAT TCCATCCGGA GTGCTTCTGC
    TGCACGGCAT GCGGCTCCTT CATCGGCGAG GGCGAATCGT ACGCCCTTGT CGAGCGTTCG
    AAGCTCTATT GTGGCCAGTG CTATGGCAAA CGTTCCTGCC AGCCGGCGGA TGCGAAGGCG
    CGGATCACAA CGGCCGGCAA GCCGATGCAC TCCATCCGTC TGGTCGAGAT ACCCAAGGAC
    GCCACGCCCG GCCTGAGGGT CGACGGAGTG GCCCTCGACG ATGGTTGTCC CACGGTCCGC
    ATTACAGAAA TTGATGTGAA TCTTACAAAT CTGCATATTG GAGATAGGAT ATTGGAGGTG
    AACGGCACAC CAGTTAGTGA TTCGTCCGTG GAACAGATCG ACAAATTGAT ACGGAGTAAT
    GAGAAGATGC TTCAGCTGAC CGTGGAGCAT GATCCGGTGC AAGTGTGCCG CTCCTGCAGC
    CAAGCGGACA TCCAGCGGGC CATGTCAGCG TCCACGTTAA TCCTGCCGCT CAGCACATCG
    GCCTCCAGTG TCGAGGTGGG CAGGGAGCGT CTGTACAAGA CACCCGGCGA ACAGGGCACG
    AAGGCCAGGA AACTGCGACA AGCCACCAAT GCCTCTACGA CAATACCACC GGCAGCTGGA
    GCCACCGCAA TGACGCAACT AAAGGAGAAG GAGCGTTGTT CCAGTCTGTC CAAGTTGCTG
    GATGAACAGC ATCAGGCACA ACAGCATTCG GCGCATCCTC AGCTCTATGA TCTGTCCAGG
    ACGCAATCCT GCCGTGTCGT CCAAAAGCCA CAACGAATCT TTCGTGCAAC TGATCTGGTT
    ATAGGAGAGA AGCTAGGCGA GGGCTTCTTT GGCAAGGTTT TCAAGGTGAC CCACCGTCAG
    AGCGGCGAGG TGATGGTCCT CAAAGAACTG CATCGTGCGG ATGAGGAAGC GCAAAGGAAT
    TTCATCAAGG AAGTGGCCGT TCTCCGTTTG CTGGACCATC GGCATGTGCT CAAATTCATT
    GGTGTTCTGT ACAAGGATAA GAAACTGCAC ATGGTCACGG AGTACGTGGC CGGTGGTTGT
    CTCAAGGAGC TGATCCACGA TCCAGCCCAA GTACTCCCTT GGCCCCAGCG CGTCCGCTTG
    GCCAGGGATA TTGCGTGCGG CATGAGCTAT CTGCACTCGA TGAACATCAT CCATCGCGAT
    TTGAACTCAA TGAATTGCCT GGTCCGTGAA GATCGCTCGG TCATAGTGGC GGATTTTGGA
    CTAGCTCGCA GTGTGGATGC GCCGCGGCTG CCAAGTGGCA ATATGACGCC AGGCGGCTAT
    GGATCAGGCG CTAACAGCGA TGCGCCCATG TCACCGAGTG GAACACTAAG GCGCAGCAAG
    AGCAGGCAGC GAAGGCAACG TTACACGGTG GTCGGGAATC CCTACTGGAT GGCACCCGAG
    ATGATGAAAG GTCTCAAATA CGACGAAAAG GTGGATGTCT TCTCCTTTGG CATTATGTTG
    TGTGAGATCA TTGGACGCGT GGAAGCCGAT CCGGATTTTA TGCCTCGCAA TTCGGACTTC
    AGTCTCAATC AGCAGGAGTT TCGCGAGAAG TTCTGCGCTC AGTGTCCAGA GCCTTTCGTT
    AAAGTGGCCT TCGTGTGCTG TGATCTTAAT CCCGATATGC GTCCGTGTTT CGAAACTCTG
    CACGTTTGGC TGCAGCGTCT CGCCGATGAT CTGGCCGCCG ATCGTGTGCC GCCAGAGCGT
    CTGCTGCACG AGATTGAGAC CTTTCAGGAA TGGTACGCCA GCTCTGAGGA CGCCTTGTCA
    CCCACCTCCC AGCGCAGCTT AAATAATCTC GATGAGTTGG TCAAGAGTGC GGTGGATTCG
    GAAATTTCGC CGGTAGAAAA AGAGAAGGAG AATATGGTGA TTAAGCCCCA GGATATACCG
    AAATCTCCGC ATCTCGGCAA GGATTTCTCG CCCAGCGGCG AGAGACTGCG TGACAGTATG
    CGGGCCAGGC GTCGCCAACG TTTCTTGGGC GCCCAGGAGG AGCGTCGTAA TCTCACGCCC
    GACACGGAGT CCAAGGAAAG GGCTTTGAAG AAGGCGCTGA AGAAATGTCG TCCGTTTGGC
    GAAAGAGGTT ACCTGGTGGA TCTGCGTGCC GGTGCCGAAT TGCAGCTAGA GGATGTCAGG
    GATTTGAACA CCTATTCCGA TGTGGACTCC AGTTGCGATA CTAGCCTAAA TTACCATGAT
    GTAAATAATC TGCCAGCAGC GCAAGAGGAT GAGAATACAG TGAAACCGGG CAAGGAAGAA
    CTGTTGGAGG AGAGCACAAA TAAACCCAGC AACCAGGAGT CACAACACCA TCGTCTGGCC
    ATCGATGATA TGCGCACCCG CCTGAATCAG TGCCGCAGCA AATTCGAGCA TTTGGAGGAG
    GCTAGTCGGC GGAACTTCAA CCAGTCGCAG CACTCCATGA AGAACTTTTT CAAAACCCCA
    CCGGTGGCCC TTAAGATGTT TCAGCGCCTG GAGCACGAGG CTGCAGCCCT GAATGGTGGC
    AACAATTGCC CACCGCCACC ACCACGTACG CAGCGCATCA ATCAGACGCC CATTTTCGGT
    CGAAAGAATC CGCCAGTGGC AATCGTCGGG CAAAAACTAC AGCATGCTGA GTCCCTAGAG
    GACCTGGCCT CCAGCGGAGT TGCCAAACAG CTGGCCACAC CAGCACCCAA ACGCAGCAAA
    GCCACAGCAA CGACCAAAGG CGGCCAGAGC AGCAATCCGC CACTTTTCCT GCCGCCTAGC
    CTTAACATAA GTGTCGCATT AAATAGCAAT GGAAATGTGA CCACAACGAC CAATACCAAT
    AGTAGTTGTC CACCATCCGC ATCAGACTGG CTGCCGAAGA AGCACAAGCT AACTTTGCCA
    CTTCCGTCGG CCCAACAGCA GCGAACGAGC AGCAATCATC GCCTGCCAAT GTGCAATAAC
    AAAGGGAAGA CACTGAAGCC ACTGCCAAGT CGCACGGGCA GTCAAGGCAT TCCCGCAAGC
    AATTGCGTCT CGCCGACGAG GAGCAGCAGG CCCGGATCAC CCACCAAGCA TCTGGCCCAG
    AGGCACACAG CCGCCACGGC GCAGCGACTG ACAAATGCGG CCGCCACTCA CCAGCAGCAG
    CATCAGCAAC AATCCTCGAA GACGACGCGC CTGAATATCC TGAGTCCGGA AAAGGTGCAT
    CGCCTGGGCG CTCGACTCAC CGATCAGAAG CAGAAAATGC GCGAGGAGGC CGCAGCGACG
    GCCTCGTCCG TCGGAGGAGC CGGTTGTGCG GCAGGAACAG CGGCTGGAAG CTTAAATGGT
    CATCGGACCA TCGGATCCTC CGGCACACCG AATTCGGCGG TCGGTGAACG GAGGAGGCGA
    GCAGCTCCAT CGCCGCCAGT TCGCACGCAT TTCAATACGC GCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162733.1
    CDS300..4007
    Translation

    Target ORF information:

    RefSeq Version NM_001169262.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169262.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCATCATC AGCAGCGCCT GCGGGCAAAT GGCGGACGAG GAGGCACTGG ATTGGGCGCA 
    GGATCAGGAC CTGTGTCCGG AGGACACTCA CCATTGTGCG CCCATTGTCG GGGTCAACTA
    CTGCCACATC CAGAGGAGCC AATCGTGATG GCTCTCGGCC AGCAATGGCA CTGCGATTGC
    TTTCGCTGCT CCGTTTGCGA GGGCCATCTG CATAACTGGT ACTTCGAACG GGAGGGCTTA
    CTGTACTGTC GTGAGGATTA CTACGGCCGA TTCGGCGATG CCTGTCAGCA GTGTATGGCC
    GTTATCACCG GACCCGTAAT GGTCGCCGGC GAGCACAAAT TCCATCCGGA GTGCTTCTGC
    TGCACGGCAT GCGGCTCCTT CATCGGCGAG GGCGAATCGT ACGCCCTTGT CGAGCGTTCG
    AAGCTCTATT GTGGCCAGTG CTATGGCAAA CGTTCCTGCC AGCCGGCGGA TGCGAAGGCG
    CGGATCACAA CGGCCGGCAA GCCGATGCAC TCCATCCGTC TGGTCGAGAT ACCCAAGGAC
    GCCACGCCCG GCCTGAGGGT CGACGGAGTG GCCCTCGACG ATGGTTGTCC CACGGTCCGC
    ATTACAGAAA TTGATGTGAA TCTTACAAAT CTGCATATTG GAGATAGGAT ATTGGAGGTG
    AACGGCACAC CAGTTAGTGA TTCGTCCGTG GAACAGATCG ACAAATTGAT ACGGAGTAAT
    GAGAAGATGC TTCAGCTGAC CGTGGAGCAT GATCCGGTGC AAGTGTGCCG CTCCTGCAGC
    CAAGCGGACA TCCAGCGGGC CATGTCAGCG TCCACGTTAA TCCTGCCGCT CAGCACATCG
    GCCTCCAGTG TCGAGGTGGG CAGGGAGCGT CTGTACAAGA CACCCGGCGA ACAGGGCACG
    AAGGCCAGGA AACTGCGACA AGCCACCAAT GCCTCTACGA CAATACCACC GGCAGCTGGA
    GCCACCGCAA TGACGCAACT AAAGGAGAAG GAGCGTTGTT CCAGTCTGTC CAAGTTGCTG
    GATGAACAGC ATCAGGCACA ACAGCATTCG GCGCATCCTC AGCTCTATGA TCTGTCCAGG
    ACGCAATCCT GCCGTGTCGT CCAAAAGCCA CAACGAATCT TTCGTGCAAC TGATCTGGTT
    ATAGGAGAGA AGCTAGGCGA GGGCTTCTTT GGCAAGGTTT TCAAGGTGAC CCACCGTCAG
    AGCGGCGAGG TGATGGTCCT CAAAGAACTG CATCGTGCGG ATGAGGAAGC GCAAAGGAAT
    TTCATCAAGG AAGTGGCCGT TCTCCGTTTG CTGGACCATC GGCATGTGCT CAAATTCATT
    GGTGTTCTGT ACAAGGATAA GAAACTGCAC ATGGTCACGG AGTACGTGGC CGGTGGTTGT
    CTCAAGGAGC TGATCCACGA TCCAGCCCAA GTACTCCCTT GGCCCCAGCG CGTCCGCTTG
    GCCAGGGATA TTGCGTGCGG CATGAGCTAT CTGCACTCGA TGAACATCAT CCATCGCGAT
    TTGAACTCAA TGAATTGCCT GGTCCGTGAA GATCGCTCGG TCATAGTGGC GGATTTTGGA
    CTAGCTCGCA GTGTGGATGC GCCGCGGCTG CCAAGTGGCA ATATGACGCC AGGCGGCTAT
    GGATCAGGCG CTAACAGCGA TGCGCCCATG TCACCGAGTG GAACACTAAG GCGCAGCAAG
    AGCAGGCAGC GAAGGCAACG TTACACGGTG GTCGGGAATC CCTACTGGAT GGCACCCGAG
    ATGATGAAAG GTCTCAAATA CGACGAAAAG GTGGATGTCT TCTCCTTTGG CATTATGTTG
    TGTGAGATCA TTGGACGCGT GGAAGCCGAT CCGGATTTTA TGCCTCGCAA TTCGGACTTC
    AGTCTCAATC AGCAGGAGTT TCGCGAGAAG TTCTGCGCTC AGTGTCCAGA GCCTTTCGTT
    AAAGTGGCCT TCGTGTGCTG TGATCTTAAT CCCGATATGC GTCCGTGTTT CGAAACTCTG
    CACGTTTGGC TGCAGCGTCT CGCCGATGAT CTGGCCGCCG ATCGTGTGCC GCCAGAGCGT
    CTGCTGCACG AGATTGAGAC CTTTCAGGAA TGGTACGCCA GCTCTGAGGA CGCCTTGTCA
    CCCACCTCCC AGCGCAGCTT AAATAATCTC GATGAGTTGG TCAAGAGTGC GGTGGATTCG
    GAAATTTCGC CGGTAGAAAA AGAGAAGGAG AATATGGTGA TTAAGCCCCA GGATATACCG
    AAATCTCCGC ATCTCGGCAA GGATTTCTCG CCCAGCGGCG AGAGACTGCG TGACAGTATG
    CGGGCCAGGC GTCGCCAACG TTTCTTGGGC GCCCAGGAGG AGCGTCGTAA TCTCACGCCC
    GACACGGAGT CCAAGGAAAG GGCTTTGAAG AAGGCGCTGA AGAAATGTCG TCCGTTTGGC
    GAAAGAGGTT ACCTGGTGGA TCTGCGTGCC GGTGCCGAAT TGCAGCTAGA GGATGTCAGG
    GATTTGAACA CCTATTCCGA TGTGGACTCC AGTTGCGATA CTAGCCTAAA TTACCATGAT
    GTAAATAATC TGCCAGCAGC GCAAGAGGAT GAGAATACAG TGAAACCGGG CAAGGAAGAA
    CTGTTGGAGG AGAGCACAAA TAAACCCAGC AACCAGGAGT CACAACACCA TCGTCTGGCC
    ATCGATGATA TGCGCACCCG CCTGAATCAG TGCCGCAGCA AATTCGAGCA TTTGGAGGAG
    GCTAGTCGGC GGAACTTCAA CCAGTCGCAG CACTCCATGA AGAACTTTTT CAAAACCCCA
    CCGGTGGCCC TTAAGATGTT TCAGCGCCTG GAGCACGAGG CTGCAGCCCT GAATGGTGGC
    AACAATTGCC CACCGCCACC ACCACGTACG CAGCGCATCA ATCAGACGCC CATTTTCGGT
    CGAAAGAATC CGCCAGTGGC AATCGTCGGG CAAAAACTAC AGCATGCTGA GTCCCTAGAG
    GACCTGGCCT CCAGCGGAGT TGCCAAACAG CTGGCCACAC CAGCACCCAA ACGCAGCAAA
    GCCACAGCAA CGACCAAAGG CGGCCAGAGC AGCAATCCGC CACTTTTCCT GCCGCCTAGC
    CTTAACATAA GTGTCGCATT AAATAGCAAT GGAAATGTGA CCACAACGAC CAATACCAAT
    AGTAGTTGTC CACCATCCGC ATCAGACTGG CTGCCGAAGA AGCACAAGCT AACTTTGCCA
    CTTCCGTCGG CCCAACAGCA GCGAACGAGC AGCAATCATC GCCTGCCAAT GTGCAATAAC
    AAAGGGAAGA CACTGAAGCC ACTGCCAAGT CGCACGGGCA GTCAAGGCAT TCCCGCAAGC
    AATTGCGTCT CGCCGACGAG GAGCAGCAGG CCCGGATCAC CCACCAAGCA TCTGGCCCAG
    AGGCACACAG CCGCCACGGC GCAGCGACTG ACAAATGCGG CCGCCACTCA CCAGCAGCAG
    CATCAGCAAC AATCCTCGAA GACGACGCGC CTGAATATCC TGAGTCCGGA AAAGGTGCAT
    CGCCTGGGCG CTCGACTCAC CGATCAGAAG CAGAAAATGC GCGAGGAGGC CGCAGCGACG
    GCCTCGTCCG TCGGAGGAGC CGGTTGTGCG GCAGGAACAG CGGCTGGAAG CTTAAATGGT
    CATCGGACCA TCGGATCCTC CGGCACACCG AATTCGGCGG TCGGTGAACG GAGGAGGCGA
    GCAGCTCCAT CGCCGCCAGT TCGCACGCAT TTCAATACGC GCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa01407
    Clone ID Related Accession (Same CDS sequence) NM_001169262.1 , NM_167326.2
    Accession Version NM_167326.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product LIM domain kinase 1, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_167326.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCATCATC AGCAGCGCCT GCGGGCAAAT GGCGGACGAG GAGGCACTGG ATTGGGCGCA 
    GGATCAGGAC CTGTGTCCGG AGGACACTCA CCATTGTGCG CCCATTGTCG GGGTCAACTA
    CTGCCACATC CAGAGGAGCC AATCGTGATG GCTCTCGGCC AGCAATGGCA CTGCGATTGC
    TTTCGCTGCT CCGTTTGCGA GGGCCATCTG CATAACTGGT ACTTCGAACG GGAGGGCTTA
    CTGTACTGTC GTGAGGATTA CTACGGCCGA TTCGGCGATG CCTGTCAGCA GTGTATGGCC
    GTTATCACCG GACCCGTAAT GGTCGCCGGC GAGCACAAAT TCCATCCGGA GTGCTTCTGC
    TGCACGGCAT GCGGCTCCTT CATCGGCGAG GGCGAATCGT ACGCCCTTGT CGAGCGTTCG
    AAGCTCTATT GTGGCCAGTG CTATGGCAAA CGTTCCTGCC AGCCGGCGGA TGCGAAGGCG
    CGGATCACAA CGGCCGGCAA GCCGATGCAC TCCATCCGTC TGGTCGAGAT ACCCAAGGAC
    GCCACGCCCG GCCTGAGGGT CGACGGAGTG GCCCTCGACG ATGGTTGTCC CACGGTCCGC
    ATTACAGAAA TTGATGTGAA TCTTACAAAT CTGCATATTG GAGATAGGAT ATTGGAGGTG
    AACGGCACAC CAGTTAGTGA TTCGTCCGTG GAACAGATCG ACAAATTGAT ACGGAGTAAT
    GAGAAGATGC TTCAGCTGAC CGTGGAGCAT GATCCGGTGC AAGTGTGCCG CTCCTGCAGC
    CAAGCGGACA TCCAGCGGGC CATGTCAGCG TCCACGTTAA TCCTGCCGCT CAGCACATCG
    GCCTCCAGTG TCGAGGTGGG CAGGGAGCGT CTGTACAAGA CACCCGGCGA ACAGGGCACG
    AAGGCCAGGA AACTGCGACA AGCCACCAAT GCCTCTACGA CAATACCACC GGCAGCTGGA
    GCCACCGCAA TGACGCAACT AAAGGAGAAG GAGCGTTGTT CCAGTCTGTC CAAGTTGCTG
    GATGAACAGC ATCAGGCACA ACAGCATTCG GCGCATCCTC AGCTCTATGA TCTGTCCAGG
    ACGCAATCCT GCCGTGTCGT CCAAAAGCCA CAACGAATCT TTCGTGCAAC TGATCTGGTT
    ATAGGAGAGA AGCTAGGCGA GGGCTTCTTT GGCAAGGTTT TCAAGGTGAC CCACCGTCAG
    AGCGGCGAGG TGATGGTCCT CAAAGAACTG CATCGTGCGG ATGAGGAAGC GCAAAGGAAT
    TTCATCAAGG AAGTGGCCGT TCTCCGTTTG CTGGACCATC GGCATGTGCT CAAATTCATT
    GGTGTTCTGT ACAAGGATAA GAAACTGCAC ATGGTCACGG AGTACGTGGC CGGTGGTTGT
    CTCAAGGAGC TGATCCACGA TCCAGCCCAA GTACTCCCTT GGCCCCAGCG CGTCCGCTTG
    GCCAGGGATA TTGCGTGCGG CATGAGCTAT CTGCACTCGA TGAACATCAT CCATCGCGAT
    TTGAACTCAA TGAATTGCCT GGTCCGTGAA GATCGCTCGG TCATAGTGGC GGATTTTGGA
    CTAGCTCGCA GTGTGGATGC GCCGCGGCTG CCAAGTGGCA ATATGACGCC AGGCGGCTAT
    GGATCAGGCG CTAACAGCGA TGCGCCCATG TCACCGAGTG GAACACTAAG GCGCAGCAAG
    AGCAGGCAGC GAAGGCAACG TTACACGGTG GTCGGGAATC CCTACTGGAT GGCACCCGAG
    ATGATGAAAG GTCTCAAATA CGACGAAAAG GTGGATGTCT TCTCCTTTGG CATTATGTTG
    TGTGAGATCA TTGGACGCGT GGAAGCCGAT CCGGATTTTA TGCCTCGCAA TTCGGACTTC
    AGTCTCAATC AGCAGGAGTT TCGCGAGAAG TTCTGCGCTC AGTGTCCAGA GCCTTTCGTT
    AAAGTGGCCT TCGTGTGCTG TGATCTTAAT CCCGATATGC GTCCGTGTTT CGAAACTCTG
    CACGTTTGGC TGCAGCGTCT CGCCGATGAT CTGGCCGCCG ATCGTGTGCC GCCAGAGCGT
    CTGCTGCACG AGATTGAGAC CTTTCAGGAA TGGTACGCCA GCTCTGAGGA CGCCTTGTCA
    CCCACCTCCC AGCGCAGCTT AAATAATCTC GATGAGTTGG TCAAGAGTGC GGTGGATTCG
    GAAATTTCGC CGGTAGAAAA AGAGAAGGAG AATATGGTGA TTAAGCCCCA GGATATACCG
    AAATCTCCGC ATCTCGGCAA GGATTTCTCG CCCAGCGGCG AGAGACTGCG TGACAGTATG
    CGGGCCAGGC GTCGCCAACG TTTCTTGGGC GCCCAGGAGG AGCGTCGTAA TCTCACGCCC
    GACACGGAGT CCAAGGAAAG GGCTTTGAAG AAGGCGCTGA AGAAATGTCG TCCGTTTGGC
    GAAAGAGGTT ACCTGGTGGA TCTGCGTGCC GGTGCCGAAT TGCAGCTAGA GGATGTCAGG
    GATTTGAACA CCTATTCCGA TGTGGACTCC AGTTGCGATA CTAGCCTAAA TTACCATGAT
    GTAAATAATC TGCCAGCAGC GCAAGAGGAT GAGAATACAG TGAAACCGGG CAAGGAAGAA
    CTGTTGGAGG AGAGCACAAA TAAACCCAGC AACCAGGAGT CACAACACCA TCGTCTGGCC
    ATCGATGATA TGCGCACCCG CCTGAATCAG TGCCGCAGCA AATTCGAGCA TTTGGAGGAG
    GCTAGTCGGC GGAACTTCAA CCAGTCGCAG CACTCCATGA AGAACTTTTT CAAAACCCCA
    CCGGTGGCCC TTAAGATGTT TCAGCGCCTG GAGCACGAGG CTGCAGCCCT GAATGGTGGC
    AACAATTGCC CACCGCCACC ACCACGTACG CAGCGCATCA ATCAGACGCC CATTTTCGGT
    CGAAAGAATC CGCCAGTGGC AATCGTCGGG CAAAAACTAC AGCATGCTGA GTCCCTAGAG
    GACCTGGCCT CCAGCGGAGT TGCCAAACAG CTGGCCACAC CAGCACCCAA ACGCAGCAAA
    GCCACAGCAA CGACCAAAGG CGGCCAGAGC AGCAATCCGC CACTTTTCCT GCCGCCTAGC
    CTTAACATAA GTGTCGCATT AAATAGCAAT GGAAATGTGA CCACAACGAC CAATACCAAT
    AGTAGTTGTC CACCATCCGC ATCAGACTGG CTGCCGAAGA AGCACAAGCT AACTTTGCCA
    CTTCCGTCGG CCCAACAGCA GCGAACGAGC AGCAATCATC GCCTGCCAAT GTGCAATAAC
    AAAGGGAAGA CACTGAAGCC ACTGCCAAGT CGCACGGGCA GTCAAGGCAT TCCCGCAAGC
    AATTGCGTCT CGCCGACGAG GAGCAGCAGG CCCGGATCAC CCACCAAGCA TCTGGCCCAG
    AGGCACACAG CCGCCACGGC GCAGCGACTG ACAAATGCGG CCGCCACTCA CCAGCAGCAG
    CATCAGCAAC AATCCTCGAA GACGACGCGC CTGAATATCC TGAGTCCGGA AAAGGTGCAT
    CGCCTGGGCG CTCGACTCAC CGATCAGAAG CAGAAAATGC GCGAGGAGGC CGCAGCGACG
    GCCTCGTCCG TCGGAGGAGC CGGTTGTGCG GCAGGAACAG CGGCTGGAAG CTTAAATGGT
    CATCGGACCA TCGGATCCTC CGGCACACCG AATTCGGCGG TCGGTGAACG GAGGAGGCGA
    GCAGCTCCAT CGCCGCCAGT TCGCACGCAT TTCAATACGC GCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_727621.1
    CDS263..3970
    Translation

    Target ORF information:

    RefSeq Version NM_167326.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_167326.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCATCATC AGCAGCGCCT GCGGGCAAAT GGCGGACGAG GAGGCACTGG ATTGGGCGCA 
    GGATCAGGAC CTGTGTCCGG AGGACACTCA CCATTGTGCG CCCATTGTCG GGGTCAACTA
    CTGCCACATC CAGAGGAGCC AATCGTGATG GCTCTCGGCC AGCAATGGCA CTGCGATTGC
    TTTCGCTGCT CCGTTTGCGA GGGCCATCTG CATAACTGGT ACTTCGAACG GGAGGGCTTA
    CTGTACTGTC GTGAGGATTA CTACGGCCGA TTCGGCGATG CCTGTCAGCA GTGTATGGCC
    GTTATCACCG GACCCGTAAT GGTCGCCGGC GAGCACAAAT TCCATCCGGA GTGCTTCTGC
    TGCACGGCAT GCGGCTCCTT CATCGGCGAG GGCGAATCGT ACGCCCTTGT CGAGCGTTCG
    AAGCTCTATT GTGGCCAGTG CTATGGCAAA CGTTCCTGCC AGCCGGCGGA TGCGAAGGCG
    CGGATCACAA CGGCCGGCAA GCCGATGCAC TCCATCCGTC TGGTCGAGAT ACCCAAGGAC
    GCCACGCCCG GCCTGAGGGT CGACGGAGTG GCCCTCGACG ATGGTTGTCC CACGGTCCGC
    ATTACAGAAA TTGATGTGAA TCTTACAAAT CTGCATATTG GAGATAGGAT ATTGGAGGTG
    AACGGCACAC CAGTTAGTGA TTCGTCCGTG GAACAGATCG ACAAATTGAT ACGGAGTAAT
    GAGAAGATGC TTCAGCTGAC CGTGGAGCAT GATCCGGTGC AAGTGTGCCG CTCCTGCAGC
    CAAGCGGACA TCCAGCGGGC CATGTCAGCG TCCACGTTAA TCCTGCCGCT CAGCACATCG
    GCCTCCAGTG TCGAGGTGGG CAGGGAGCGT CTGTACAAGA CACCCGGCGA ACAGGGCACG
    AAGGCCAGGA AACTGCGACA AGCCACCAAT GCCTCTACGA CAATACCACC GGCAGCTGGA
    GCCACCGCAA TGACGCAACT AAAGGAGAAG GAGCGTTGTT CCAGTCTGTC CAAGTTGCTG
    GATGAACAGC ATCAGGCACA ACAGCATTCG GCGCATCCTC AGCTCTATGA TCTGTCCAGG
    ACGCAATCCT GCCGTGTCGT CCAAAAGCCA CAACGAATCT TTCGTGCAAC TGATCTGGTT
    ATAGGAGAGA AGCTAGGCGA GGGCTTCTTT GGCAAGGTTT TCAAGGTGAC CCACCGTCAG
    AGCGGCGAGG TGATGGTCCT CAAAGAACTG CATCGTGCGG ATGAGGAAGC GCAAAGGAAT
    TTCATCAAGG AAGTGGCCGT TCTCCGTTTG CTGGACCATC GGCATGTGCT CAAATTCATT
    GGTGTTCTGT ACAAGGATAA GAAACTGCAC ATGGTCACGG AGTACGTGGC CGGTGGTTGT
    CTCAAGGAGC TGATCCACGA TCCAGCCCAA GTACTCCCTT GGCCCCAGCG CGTCCGCTTG
    GCCAGGGATA TTGCGTGCGG CATGAGCTAT CTGCACTCGA TGAACATCAT CCATCGCGAT
    TTGAACTCAA TGAATTGCCT GGTCCGTGAA GATCGCTCGG TCATAGTGGC GGATTTTGGA
    CTAGCTCGCA GTGTGGATGC GCCGCGGCTG CCAAGTGGCA ATATGACGCC AGGCGGCTAT
    GGATCAGGCG CTAACAGCGA TGCGCCCATG TCACCGAGTG GAACACTAAG GCGCAGCAAG
    AGCAGGCAGC GAAGGCAACG TTACACGGTG GTCGGGAATC CCTACTGGAT GGCACCCGAG
    ATGATGAAAG GTCTCAAATA CGACGAAAAG GTGGATGTCT TCTCCTTTGG CATTATGTTG
    TGTGAGATCA TTGGACGCGT GGAAGCCGAT CCGGATTTTA TGCCTCGCAA TTCGGACTTC
    AGTCTCAATC AGCAGGAGTT TCGCGAGAAG TTCTGCGCTC AGTGTCCAGA GCCTTTCGTT
    AAAGTGGCCT TCGTGTGCTG TGATCTTAAT CCCGATATGC GTCCGTGTTT CGAAACTCTG
    CACGTTTGGC TGCAGCGTCT CGCCGATGAT CTGGCCGCCG ATCGTGTGCC GCCAGAGCGT
    CTGCTGCACG AGATTGAGAC CTTTCAGGAA TGGTACGCCA GCTCTGAGGA CGCCTTGTCA
    CCCACCTCCC AGCGCAGCTT AAATAATCTC GATGAGTTGG TCAAGAGTGC GGTGGATTCG
    GAAATTTCGC CGGTAGAAAA AGAGAAGGAG AATATGGTGA TTAAGCCCCA GGATATACCG
    AAATCTCCGC ATCTCGGCAA GGATTTCTCG CCCAGCGGCG AGAGACTGCG TGACAGTATG
    CGGGCCAGGC GTCGCCAACG TTTCTTGGGC GCCCAGGAGG AGCGTCGTAA TCTCACGCCC
    GACACGGAGT CCAAGGAAAG GGCTTTGAAG AAGGCGCTGA AGAAATGTCG TCCGTTTGGC
    GAAAGAGGTT ACCTGGTGGA TCTGCGTGCC GGTGCCGAAT TGCAGCTAGA GGATGTCAGG
    GATTTGAACA CCTATTCCGA TGTGGACTCC AGTTGCGATA CTAGCCTAAA TTACCATGAT
    GTAAATAATC TGCCAGCAGC GCAAGAGGAT GAGAATACAG TGAAACCGGG CAAGGAAGAA
    CTGTTGGAGG AGAGCACAAA TAAACCCAGC AACCAGGAGT CACAACACCA TCGTCTGGCC
    ATCGATGATA TGCGCACCCG CCTGAATCAG TGCCGCAGCA AATTCGAGCA TTTGGAGGAG
    GCTAGTCGGC GGAACTTCAA CCAGTCGCAG CACTCCATGA AGAACTTTTT CAAAACCCCA
    CCGGTGGCCC TTAAGATGTT TCAGCGCCTG GAGCACGAGG CTGCAGCCCT GAATGGTGGC
    AACAATTGCC CACCGCCACC ACCACGTACG CAGCGCATCA ATCAGACGCC CATTTTCGGT
    CGAAAGAATC CGCCAGTGGC AATCGTCGGG CAAAAACTAC AGCATGCTGA GTCCCTAGAG
    GACCTGGCCT CCAGCGGAGT TGCCAAACAG CTGGCCACAC CAGCACCCAA ACGCAGCAAA
    GCCACAGCAA CGACCAAAGG CGGCCAGAGC AGCAATCCGC CACTTTTCCT GCCGCCTAGC
    CTTAACATAA GTGTCGCATT AAATAGCAAT GGAAATGTGA CCACAACGAC CAATACCAAT
    AGTAGTTGTC CACCATCCGC ATCAGACTGG CTGCCGAAGA AGCACAAGCT AACTTTGCCA
    CTTCCGTCGG CCCAACAGCA GCGAACGAGC AGCAATCATC GCCTGCCAAT GTGCAATAAC
    AAAGGGAAGA CACTGAAGCC ACTGCCAAGT CGCACGGGCA GTCAAGGCAT TCCCGCAAGC
    AATTGCGTCT CGCCGACGAG GAGCAGCAGG CCCGGATCAC CCACCAAGCA TCTGGCCCAG
    AGGCACACAG CCGCCACGGC GCAGCGACTG ACAAATGCGG CCGCCACTCA CCAGCAGCAG
    CATCAGCAAC AATCCTCGAA GACGACGCGC CTGAATATCC TGAGTCCGGA AAAGGTGCAT
    CGCCTGGGCG CTCGACTCAC CGATCAGAAG CAGAAAATGC GCGAGGAGGC CGCAGCGACG
    GCCTCGTCCG TCGGAGGAGC CGGTTGTGCG GCAGGAACAG CGGCTGGAAG CTTAAATGGT
    CATCGGACCA TCGGATCCTC CGGCACACCG AATTCGGCGG TCGGTGAACG GAGGAGGCGA
    GCAGCTCCAT CGCCGCCAGT TCGCACGCAT TTCAATACGC GCTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02798
    Clone ID Related Accession (Same CDS sequence) NM_001169263.1
    Accession Version NM_001169263.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product LIM domain kinase 1, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGGAAC AGTGCTGTGG GGCGCCTTAT AGAATTGATG TGAATCTTAC AAATCTGCAT 
    ATTGGAGATA GGATATTGGA GGTGAACGGC ACACCAGTTA GTGATTCGTC CGTGGAACAG
    ATCGACAAAT TGATACGGAG TAATGAGAAG ATGCTTCAGC TGACCGTGGA GCATGATCCG
    GTGCAAGTGT GCCGCTCCTG CAGCCAAGCG GACATCCAGC GGGCCATGTC AGCGTCCACG
    TTAATCCTGC CGCTCAGCAC ATCGGCCTCC AGTGTCGAGG TGGGCAGGGA GCGTCTGTAC
    AAGACACCCG GCGAACAGGG CACGAAGGCC AGGAAACTGC GACAAGCCAC CAATGCCTCT
    ACGACAATAC CACCGGCAGC TGGAGCCACC GCAATGACGC AACTAAAGGA GAAGGAGCGT
    TGTTCCAGTC TGTCCAAGTT GCTGGATGAA CAGCATCAGG CACAACAGCA TTCGGCGCAT
    CCTCAGCTCT ATGATCTGTC CAGGACGCAA TCCTGCCGTG TCGTCCAAAA GCCACAACGA
    ATCTTTCGTG CAACTGATCT GGTTATAGGA GAGAAGCTAG GCGAGGGCTT CTTTGGCAAG
    GTTTTCAAGG TGACCCACCG TCAGAGCGGC GAGGTGATGG TCCTCAAAGA ACTGCATCGT
    GCGGATGAGG AAGCGCAAAG GAATTTCATC AAGGAAGTGG CCGTTCTCCG TTTGCTGGAC
    CATCGGCATG TGCTCAAATT CATTGGTGTT CTGTACAAGG ATAAGAAACT GCACATGGTC
    ACGGAGTACG TGGCCGGTGG TTGTCTCAAG GAGCTGATCC ACGATCCAGC CCAAGTACTC
    CCTTGGCCCC AGCGCGTCCG CTTGGCCAGG GATATTGCGT GCGGCATGAG CTATCTGCAC
    TCGATGAACA TCATCCATCG CGATTTGAAC TCAATGAATT GCCTGGTCCG TGAAGATCGC
    TCGGTCATAG TGGCGGATTT TGGACTAGCT CGCAGTGTGG ATGCGCCGCG GCTGCCAAGT
    GGCAATATGA CGCCAGGCGG CTATGGATCA GGCGCTAACA GCGATGCGCC CATGTCACCG
    AGTGGAACAC TAAGGCGCAG CAAGAGCAGG CAGCGAAGGC AACGTTACAC GGTGGTCGGG
    AATCCCTACT GGATGGCACC CGAGATGATG AAAGGTCTCA AATACGACGA AAAGGTGGAT
    GTCTTCTCCT TTGGCATTAT GTTGTGTGAG ATCATTGGAC GCGTGGAAGC CGATCCGGAT
    TTTATGCCTC GCAATTCGGA CTTCAGTCTC AATCAGCAGG AGTTTCGCGA GAAGTTCTGC
    GCTCAGTGTC CAGAGCCTTT CGTTAAAGTG GCCTTCGTGT GCTGTGATCT TAATCCCGAT
    ATGCGTCCGT GTTTCGAAAC TCTGCACGTT TGGCTGCAGC GTCTCGCCGA TGATCTGGCC
    GCCGATCGTG TGCCGCCAGA GCGTCTGCTG CACGAGATTG AGACCTTTCA GGAATGGTAC
    GCCAGCTCTG AGGACGCCTT GTCACCCACC TCCCAGCGCA GCTTAAATAA TCTCGATGAG
    TTGGTCAAGA GTGCGGTGGA TTCGGAAATT TCGCCGGTAG AAAAAGAGAA GGAGAATATG
    GTGATTAAGC CCCAGGATAT ACCGAAATCT CCGCATCTCG GCAAGGATTT CTCGCCCAGC
    GGCGAGAGAC TGCGTGACAG TATGCGGGCC AGGCGTCGCC AACGTTTCTT GGGCGCCCAG
    GAGGAGCGTC GTAATCTCAC GCCCGACACG GAGTCCAAGG AAAGGGCTTT GAAGAAGGCG
    CTGAAGAAAT GTCGTCCGTT TGGCGAAAGA GGTTACCTGG TGGATCTGCG TGCCGGTGCC
    GAATTGCAGC TAGAGGATGT CAGGGATTTG AACACCTATT CCGATGTGGA CTCCAGTTGC
    GATACTAGCC TAAATTACCA TGATGTAAAT AATCTGCCAG CAGCGCAAGA GGATGAGAAT
    ACAGTGAAAC CGGGCAAGGA AGAACTGTTG GAGGAGAGCA CAAATAAACC CAGCAACCAG
    GAGTCACAAC ACCATCGTCT GGCCATCGAT GATATGCGCA CCCGCCTGAA TCAGTGCCGC
    AGCAAATTCG AGCATTTGGA GGAGGCTAGT CGGCGGAACT TCAACCAGTC GCAGCACTCC
    ATGAAGAACT TTTTCAAAAC CCCACCGGTG GCCCTTAAGA TGTTTCAGCG CCTGGAGCAC
    GAGGCTGCAG CCCTGAATGG TGGCAACAAT TGCCCACCGC CACCACCACG TACGCAGCGC
    ATCAATCAGA CGCCCATTTT CGGTCGAAAG AATCCGCCAG TGGCAATCGT CGGGCAAAAA
    CTACAGCATG CTGAGTCCCT AGAGGACCTG GCCTCCAGCG GAGTTGCCAA ACAGCTGGCC
    ACACCAGCAC CCAAACGCAG CAAAGCCACA GCAACGACCA AAGGCGGCCA GAGCAGCAAT
    CCGCCACTTT TCCTGCCGCC TAGCCTTAAC ATAAGTGTCG CATTAAATAG CAATGGAAAT
    GTGACCACAA CGACCAATAC CAATAGTAGT TGTCCACCAT CCGCATCAGA CTGGCTGCCG
    AAGAAGCACA AGCTAACTTT GCCACTTCCG TCGGCCCAAC AGCAGCGAAC GAGCAGCAAT
    CATCGCCTGC CAATGTGCAA TAACAAAGGG AAGACACTGA AGCCACTGCC AAGTCGCACG
    GGCAGTCAAG GCATTCCCGC AAGCAATTGC GTCTCGCCGA CGAGGAGCAG CAGGCCCGGA
    TCACCCACCA AGCATCTGGC CCAGAGGCAC ACAGCCGCCA CGGCGCAGCG ACTGACAAAT
    GCGGCCGCCA CTCACCAGCA GCAGCATCAG CAACAATCCT CGAAGACGAC GCGCCTGAAT
    ATCCTGAGTC CGGAAAAGGT GCATCGCCTG GGCGCTCGAC TCACCGATCA GAAGCAGAAA
    ATGCGCGAGG AGGCCGCAGC GACGGCCTCG TCCGTCGGAG GAGCCGGTTG TGCGGCAGGA
    ACAGCGGCTG GAAGCTTAAA TGGTCATCGG ACCATCGGAT CCTCCGGCAC ACCGAATTCG
    GCGGTCGGTG AACGGAGGAG GCGAGCAGCT CCATCGCCGC CAGTTCGCAC GCATTTCAAT
    ACGCGCTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162734.1
    CDS62..3193
    Translation

    Target ORF information:

    RefSeq Version NM_001169263.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster LIM domain kinase 1 (LIMK1), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169263.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCGGAAC AGTGCTGTGG GGCGCCTTAT AGAATTGATG TGAATCTTAC AAATCTGCAT 
    ATTGGAGATA GGATATTGGA GGTGAACGGC ACACCAGTTA GTGATTCGTC CGTGGAACAG
    ATCGACAAAT TGATACGGAG TAATGAGAAG ATGCTTCAGC TGACCGTGGA GCATGATCCG
    GTGCAAGTGT GCCGCTCCTG CAGCCAAGCG GACATCCAGC GGGCCATGTC AGCGTCCACG
    TTAATCCTGC CGCTCAGCAC ATCGGCCTCC AGTGTCGAGG TGGGCAGGGA GCGTCTGTAC
    AAGACACCCG GCGAACAGGG CACGAAGGCC AGGAAACTGC GACAAGCCAC CAATGCCTCT
    ACGACAATAC CACCGGCAGC TGGAGCCACC GCAATGACGC AACTAAAGGA GAAGGAGCGT
    TGTTCCAGTC TGTCCAAGTT GCTGGATGAA CAGCATCAGG CACAACAGCA TTCGGCGCAT
    CCTCAGCTCT ATGATCTGTC CAGGACGCAA TCCTGCCGTG TCGTCCAAAA GCCACAACGA
    ATCTTTCGTG CAACTGATCT GGTTATAGGA GAGAAGCTAG GCGAGGGCTT CTTTGGCAAG
    GTTTTCAAGG TGACCCACCG TCAGAGCGGC GAGGTGATGG TCCTCAAAGA ACTGCATCGT
    GCGGATGAGG AAGCGCAAAG GAATTTCATC AAGGAAGTGG CCGTTCTCCG TTTGCTGGAC
    CATCGGCATG TGCTCAAATT CATTGGTGTT CTGTACAAGG ATAAGAAACT GCACATGGTC
    ACGGAGTACG TGGCCGGTGG TTGTCTCAAG GAGCTGATCC ACGATCCAGC CCAAGTACTC
    CCTTGGCCCC AGCGCGTCCG CTTGGCCAGG GATATTGCGT GCGGCATGAG CTATCTGCAC
    TCGATGAACA TCATCCATCG CGATTTGAAC TCAATGAATT GCCTGGTCCG TGAAGATCGC
    TCGGTCATAG TGGCGGATTT TGGACTAGCT CGCAGTGTGG ATGCGCCGCG GCTGCCAAGT
    GGCAATATGA CGCCAGGCGG CTATGGATCA GGCGCTAACA GCGATGCGCC CATGTCACCG
    AGTGGAACAC TAAGGCGCAG CAAGAGCAGG CAGCGAAGGC AACGTTACAC GGTGGTCGGG
    AATCCCTACT GGATGGCACC CGAGATGATG AAAGGTCTCA AATACGACGA AAAGGTGGAT
    GTCTTCTCCT TTGGCATTAT GTTGTGTGAG ATCATTGGAC GCGTGGAAGC CGATCCGGAT
    TTTATGCCTC GCAATTCGGA CTTCAGTCTC AATCAGCAGG AGTTTCGCGA GAAGTTCTGC
    GCTCAGTGTC CAGAGCCTTT CGTTAAAGTG GCCTTCGTGT GCTGTGATCT TAATCCCGAT
    ATGCGTCCGT GTTTCGAAAC TCTGCACGTT TGGCTGCAGC GTCTCGCCGA TGATCTGGCC
    GCCGATCGTG TGCCGCCAGA GCGTCTGCTG CACGAGATTG AGACCTTTCA GGAATGGTAC
    GCCAGCTCTG AGGACGCCTT GTCACCCACC TCCCAGCGCA GCTTAAATAA TCTCGATGAG
    TTGGTCAAGA GTGCGGTGGA TTCGGAAATT TCGCCGGTAG AAAAAGAGAA GGAGAATATG
    GTGATTAAGC CCCAGGATAT ACCGAAATCT CCGCATCTCG GCAAGGATTT CTCGCCCAGC
    GGCGAGAGAC TGCGTGACAG TATGCGGGCC AGGCGTCGCC AACGTTTCTT GGGCGCCCAG
    GAGGAGCGTC GTAATCTCAC GCCCGACACG GAGTCCAAGG AAAGGGCTTT GAAGAAGGCG
    CTGAAGAAAT GTCGTCCGTT TGGCGAAAGA GGTTACCTGG TGGATCTGCG TGCCGGTGCC
    GAATTGCAGC TAGAGGATGT CAGGGATTTG AACACCTATT CCGATGTGGA CTCCAGTTGC
    GATACTAGCC TAAATTACCA TGATGTAAAT AATCTGCCAG CAGCGCAAGA GGATGAGAAT
    ACAGTGAAAC CGGGCAAGGA AGAACTGTTG GAGGAGAGCA CAAATAAACC CAGCAACCAG
    GAGTCACAAC ACCATCGTCT GGCCATCGAT GATATGCGCA CCCGCCTGAA TCAGTGCCGC
    AGCAAATTCG AGCATTTGGA GGAGGCTAGT CGGCGGAACT TCAACCAGTC GCAGCACTCC
    ATGAAGAACT TTTTCAAAAC CCCACCGGTG GCCCTTAAGA TGTTTCAGCG CCTGGAGCAC
    GAGGCTGCAG CCCTGAATGG TGGCAACAAT TGCCCACCGC CACCACCACG TACGCAGCGC
    ATCAATCAGA CGCCCATTTT CGGTCGAAAG AATCCGCCAG TGGCAATCGT CGGGCAAAAA
    CTACAGCATG CTGAGTCCCT AGAGGACCTG GCCTCCAGCG GAGTTGCCAA ACAGCTGGCC
    ACACCAGCAC CCAAACGCAG CAAAGCCACA GCAACGACCA AAGGCGGCCA GAGCAGCAAT
    CCGCCACTTT TCCTGCCGCC TAGCCTTAAC ATAAGTGTCG CATTAAATAG CAATGGAAAT
    GTGACCACAA CGACCAATAC CAATAGTAGT TGTCCACCAT CCGCATCAGA CTGGCTGCCG
    AAGAAGCACA AGCTAACTTT GCCACTTCCG TCGGCCCAAC AGCAGCGAAC GAGCAGCAAT
    CATCGCCTGC CAATGTGCAA TAACAAAGGG AAGACACTGA AGCCACTGCC AAGTCGCACG
    GGCAGTCAAG GCATTCCCGC AAGCAATTGC GTCTCGCCGA CGAGGAGCAG CAGGCCCGGA
    TCACCCACCA AGCATCTGGC CCAGAGGCAC ACAGCCGCCA CGGCGCAGCG ACTGACAAAT
    GCGGCCGCCA CTCACCAGCA GCAGCATCAG CAACAATCCT CGAAGACGAC GCGCCTGAAT
    ATCCTGAGTC CGGAAAAGGT GCATCGCCTG GGCGCTCGAC TCACCGATCA GAAGCAGAAA
    ATGCGCGAGG AGGCCGCAGC GACGGCCTCG TCCGTCGGAG GAGCCGGTTG TGCGGCAGGA
    ACAGCGGCTG GAAGCTTAAA TGGTCATCGG ACCATCGGAT CCTCCGGCAC ACCGAATTCG
    GCGGTCGGTG AACGGAGGAG GCGAGCAGCT CCATCGCCGC CAGTTCGCAC GCATTTCAAT
    ACGCGCTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.