Karl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Karl
Entrez Gene ID	32131
Full Name	CG4139 gene product from transcript CG4139-RC
Synonyms	CG4139,Dmel\CG4139,karl
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10E3-10E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_132520.4	NP_572748.2	karl, isoform B
NM_167303.3	NP_727559.1	karl, isoform A
NM_001298208.1	NP_001285137.1	karl, isoform C
NM_001298209.1	NP_001285138.1	karl, isoform D

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000790	XP_311332.4
Drosophila melanogaster (fruit fly)	Karl	NP_572748.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Karl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Karl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01320	NM_167303.3 Latest version!	Drosophila melanogaster karl, transcript variant A (Karl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa01320	NM_001298208.1 Latest version!	Drosophila melanogaster karl, transcript variant C (Karl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa01320	NM_001298209.1 Latest version!	Drosophila melanogaster karl, transcript variant D (Karl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa01320	NM_132520.4 Latest version!	Drosophila melanogaster karl, transcript variant B (Karl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Karl ( NM_132520.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572748.2 Drosophila melanogaster karl, transcript variant B (Karl), mRNA.

Price & Availability↑

CloneID	OFa01320
Clone ID Related Accession (Same CDS sequence)	NM_132520.4 , NM_167303.3 , NM_001298208.1 , NM_001298209.1
Accession Version	NM_132520.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	karl, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132520.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGGA TCATGGGCAG GAATGGATCG CTCCTACTGA CCGCCGTCCT CATAATTATC 
CTGGATGTTT TGCTTGCACC AGTGGGTGCC AACCTGTGGA CACGACACAA TAGCAATGCC 
TATCAAACCA GGAGGCGCTC AGGTCCCAGC AATCGGTGTC CCAAGGTGGG AGCCATTAAG 
AACTTCGATT TGGAGCGCAT GATGGGCTGT TGGCACGTGG TTCAGTATTA CGCATCCACA 
GAGGAACTTC CGGAGTACGC GTGCATGCGC AGTCACTTTT CCTTTTCCAA AGAGGATCAG 
CATATAACGA TGAACTTTAG CTACATATTT GCGGAGGATC CGCTGCGCGA GAAGCTCGTG 
GGCAACATCA CCTGGATGAT ACCCAAATTC CAAGAGCCAG GACATTGGCA GCACACGGAG 
GACATATATG AGGGCATCTA CAACACATAC GTTCTGGACA CGGACTACGA CACCTGGGGC 
CTGGTGATGC ACTGCGCCGA GAAGAAGAAG CAACCACGTT ACCTGTCCGC CCTGCTTTTG 
TCGCGGAAAA CATCGTTGGC CGACAACGAG ATCAGCTTTC TGCGCGGCAA GTTGCCGCAG 
GATATCGACA CATCCTTTAT GTTTAACATC GGGCAGGAAT CGTGCGACAA TCTAATGGAA 
TCTAGTCGCG ACGATCCACT GGCCTATGTT GTGAATGGAC GCAAGAGAGC CAAGGAAATC 
TTCAAAATTA TCAATAAGCC TGACGGCAGA GTGCGCGCTA GTAGCGAAGC GAGAAGCTAT 
GAGCAAAGCG AGAAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572748.2
CDS	96..896
Translation	MERIMGRNGSLLLTAVLIIILDVLLAPVGANLWTRHNSNAYQTRRRSGPSNRCPKVGAIKNFDLERMMGCWHVVQYYASTEELPEYACMRSHFSFSKEDQHITMNFSYIFAEDPLREKLVGNITWMIPKFQEPGHWQHTEDIYEGIYNTYVLDTDYDTWGLVMHCAEKKKQPRYLSALLLSRKTSLADNEISFLRGKLPQDIDTSFMFNIGQESCDNLMESSRDDPLAYVVNGRKRAKEIFKIINKPDGRVRASSEARSYEQSEKL

Target ORF information:

RefSeq Version	NM_132520.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster karl, transcript variant B (Karl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132520.4

ORF Insert Sequence:

ATGGAGAGGA TCATGGGCAG GAATGGATCG CTCCTACTGA CCGCCGTCCT CATAATTATC 
CTGGATGTTT TGCTTGCACC AGTGGGTGCC AACCTGTGGA CACGACACAA TAGCAATGCC 
TATCAAACCA GGAGGCGCTC AGGTCCCAGC AATCGGTGTC CCAAGGTGGG AGCCATTAAG 
AACTTCGATT TGGAGCGCAT GATGGGCTGT TGGCACGTGG TTCAGTATTA CGCATCCACA 
GAGGAACTTC CGGAGTACGC GTGCATGCGC AGTCACTTTT CCTTTTCCAA AGAGGATCAG 
CATATAACGA TGAACTTTAG CTACATATTT GCGGAGGATC CGCTGCGCGA GAAGCTCGTG 
GGCAACATCA CCTGGATGAT ACCCAAATTC CAAGAGCCAG GACATTGGCA GCACACGGAG 
GACATATATG AGGGCATCTA CAACACATAC GTTCTGGACA CGGACTACGA CACCTGGGGC 
CTGGTGATGC ACTGCGCCGA GAAGAAGAAG CAACCACGTT ACCTGTCCGC CCTGCTTTTG 
TCGCGGAAAA CATCGTTGGC CGACAACGAG ATCAGCTTTC TGCGCGGCAA GTTGCCGCAG 
GATATCGACA CATCCTTTAT GTTTAACATC GGGCAGGAAT CGTGCGACAA TCTAATGGAA 
TCTAGTCGCG ACGATCCACT GGCCTATGTT GTGAATGGAC GCAAGAGAGC CAAGGAAATC 
TTCAAAATTA TCAATAAGCC TGACGGCAGA GTGCGCGCTA GTAGCGAAGC GAGAAGCTAT 
GAGCAAAGCG AGAAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Karl ( NM_167303.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727559.1 Drosophila melanogaster karl, transcript variant A (Karl), mRNA.

Price & Availability↑

CloneID	OFa01320
Clone ID Related Accession (Same CDS sequence)	NM_132520.4 , NM_167303.3 , NM_001298208.1 , NM_001298209.1
Accession Version	NM_167303.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	karl, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167303.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGGA TCATGGGCAG GAATGGATCG CTCCTACTGA CCGCCGTCCT CATAATTATC 
CTGGATGTTT TGCTTGCACC AGTGGGTGCC AACCTGTGGA CACGACACAA TAGCAATGCC 
TATCAAACCA GGAGGCGCTC AGGTCCCAGC AATCGGTGTC CCAAGGTGGG AGCCATTAAG 
AACTTCGATT TGGAGCGCAT GATGGGCTGT TGGCACGTGG TTCAGTATTA CGCATCCACA 
GAGGAACTTC CGGAGTACGC GTGCATGCGC AGTCACTTTT CCTTTTCCAA AGAGGATCAG 
CATATAACGA TGAACTTTAG CTACATATTT GCGGAGGATC CGCTGCGCGA GAAGCTCGTG 
GGCAACATCA CCTGGATGAT ACCCAAATTC CAAGAGCCAG GACATTGGCA GCACACGGAG 
GACATATATG AGGGCATCTA CAACACATAC GTTCTGGACA CGGACTACGA CACCTGGGGC 
CTGGTGATGC ACTGCGCCGA GAAGAAGAAG CAACCACGTT ACCTGTCCGC CCTGCTTTTG 
TCGCGGAAAA CATCGTTGGC CGACAACGAG ATCAGCTTTC TGCGCGGCAA GTTGCCGCAG 
GATATCGACA CATCCTTTAT GTTTAACATC GGGCAGGAAT CGTGCGACAA TCTAATGGAA 
TCTAGTCGCG ACGATCCACT GGCCTATGTT GTGAATGGAC GCAAGAGAGC CAAGGAAATC 
TTCAAAATTA TCAATAAGCC TGACGGCAGA GTGCGCGCTA GTAGCGAAGC GAGAAGCTAT 
GAGCAAAGCG AGAAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727559.1
CDS	96..896
Translation	MERIMGRNGSLLLTAVLIIILDVLLAPVGANLWTRHNSNAYQTRRRSGPSNRCPKVGAIKNFDLERMMGCWHVVQYYASTEELPEYACMRSHFSFSKEDQHITMNFSYIFAEDPLREKLVGNITWMIPKFQEPGHWQHTEDIYEGIYNTYVLDTDYDTWGLVMHCAEKKKQPRYLSALLLSRKTSLADNEISFLRGKLPQDIDTSFMFNIGQESCDNLMESSRDDPLAYVVNGRKRAKEIFKIINKPDGRVRASSEARSYEQSEKL

Target ORF information:

RefSeq Version	NM_167303.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster karl, transcript variant A (Karl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167303.3

ORF Insert Sequence:

ATGGAGAGGA TCATGGGCAG GAATGGATCG CTCCTACTGA CCGCCGTCCT CATAATTATC 
CTGGATGTTT TGCTTGCACC AGTGGGTGCC AACCTGTGGA CACGACACAA TAGCAATGCC 
TATCAAACCA GGAGGCGCTC AGGTCCCAGC AATCGGTGTC CCAAGGTGGG AGCCATTAAG 
AACTTCGATT TGGAGCGCAT GATGGGCTGT TGGCACGTGG TTCAGTATTA CGCATCCACA 
GAGGAACTTC CGGAGTACGC GTGCATGCGC AGTCACTTTT CCTTTTCCAA AGAGGATCAG 
CATATAACGA TGAACTTTAG CTACATATTT GCGGAGGATC CGCTGCGCGA GAAGCTCGTG 
GGCAACATCA CCTGGATGAT ACCCAAATTC CAAGAGCCAG GACATTGGCA GCACACGGAG 
GACATATATG AGGGCATCTA CAACACATAC GTTCTGGACA CGGACTACGA CACCTGGGGC 
CTGGTGATGC ACTGCGCCGA GAAGAAGAAG CAACCACGTT ACCTGTCCGC CCTGCTTTTG 
TCGCGGAAAA CATCGTTGGC CGACAACGAG ATCAGCTTTC TGCGCGGCAA GTTGCCGCAG 
GATATCGACA CATCCTTTAT GTTTAACATC GGGCAGGAAT CGTGCGACAA TCTAATGGAA 
TCTAGTCGCG ACGATCCACT GGCCTATGTT GTGAATGGAC GCAAGAGAGC CAAGGAAATC 
TTCAAAATTA TCAATAAGCC TGACGGCAGA GTGCGCGCTA GTAGCGAAGC GAGAAGCTAT 
GAGCAAAGCG AGAAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Karl ( NM_001298208.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285137.1 Drosophila melanogaster karl, transcript variant C (Karl), mRNA.

Price & Availability↑

CloneID	OFa01320
Clone ID Related Accession (Same CDS sequence)	NM_132520.4 , NM_167303.3 , NM_001298208.1 , NM_001298209.1
Accession Version	NM_001298208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	karl, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGGA TCATGGGCAG GAATGGATCG CTCCTACTGA CCGCCGTCCT CATAATTATC 
CTGGATGTTT TGCTTGCACC AGTGGGTGCC AACCTGTGGA CACGACACAA TAGCAATGCC 
TATCAAACCA GGAGGCGCTC AGGTCCCAGC AATCGGTGTC CCAAGGTGGG AGCCATTAAG 
AACTTCGATT TGGAGCGCAT GATGGGCTGT TGGCACGTGG TTCAGTATTA CGCATCCACA 
GAGGAACTTC CGGAGTACGC GTGCATGCGC AGTCACTTTT CCTTTTCCAA AGAGGATCAG 
CATATAACGA TGAACTTTAG CTACATATTT GCGGAGGATC CGCTGCGCGA GAAGCTCGTG 
GGCAACATCA CCTGGATGAT ACCCAAATTC CAAGAGCCAG GACATTGGCA GCACACGGAG 
GACATATATG AGGGCATCTA CAACACATAC GTTCTGGACA CGGACTACGA CACCTGGGGC 
CTGGTGATGC ACTGCGCCGA GAAGAAGAAG CAACCACGTT ACCTGTCCGC CCTGCTTTTG 
TCGCGGAAAA CATCGTTGGC CGACAACGAG ATCAGCTTTC TGCGCGGCAA GTTGCCGCAG 
GATATCGACA CATCCTTTAT GTTTAACATC GGGCAGGAAT CGTGCGACAA TCTAATGGAA 
TCTAGTCGCG ACGATCCACT GGCCTATGTT GTGAATGGAC GCAAGAGAGC CAAGGAAATC 
TTCAAAATTA TCAATAAGCC TGACGGCAGA GTGCGCGCTA GTAGCGAAGC GAGAAGCTAT 
GAGCAAAGCG AGAAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285137.1
CDS	1125..1925
Translation	MERIMGRNGSLLLTAVLIIILDVLLAPVGANLWTRHNSNAYQTRRRSGPSNRCPKVGAIKNFDLERMMGCWHVVQYYASTEELPEYACMRSHFSFSKEDQHITMNFSYIFAEDPLREKLVGNITWMIPKFQEPGHWQHTEDIYEGIYNTYVLDTDYDTWGLVMHCAEKKKQPRYLSALLLSRKTSLADNEISFLRGKLPQDIDTSFMFNIGQESCDNLMESSRDDPLAYVVNGRKRAKEIFKIINKPDGRVRASSEARSYEQSEKL

Target ORF information:

RefSeq Version	NM_001298208.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster karl, transcript variant C (Karl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298208.1

ORF Insert Sequence:

ATGGAGAGGA TCATGGGCAG GAATGGATCG CTCCTACTGA CCGCCGTCCT CATAATTATC 
CTGGATGTTT TGCTTGCACC AGTGGGTGCC AACCTGTGGA CACGACACAA TAGCAATGCC 
TATCAAACCA GGAGGCGCTC AGGTCCCAGC AATCGGTGTC CCAAGGTGGG AGCCATTAAG 
AACTTCGATT TGGAGCGCAT GATGGGCTGT TGGCACGTGG TTCAGTATTA CGCATCCACA 
GAGGAACTTC CGGAGTACGC GTGCATGCGC AGTCACTTTT CCTTTTCCAA AGAGGATCAG 
CATATAACGA TGAACTTTAG CTACATATTT GCGGAGGATC CGCTGCGCGA GAAGCTCGTG 
GGCAACATCA CCTGGATGAT ACCCAAATTC CAAGAGCCAG GACATTGGCA GCACACGGAG 
GACATATATG AGGGCATCTA CAACACATAC GTTCTGGACA CGGACTACGA CACCTGGGGC 
CTGGTGATGC ACTGCGCCGA GAAGAAGAAG CAACCACGTT ACCTGTCCGC CCTGCTTTTG 
TCGCGGAAAA CATCGTTGGC CGACAACGAG ATCAGCTTTC TGCGCGGCAA GTTGCCGCAG 
GATATCGACA CATCCTTTAT GTTTAACATC GGGCAGGAAT CGTGCGACAA TCTAATGGAA 
TCTAGTCGCG ACGATCCACT GGCCTATGTT GTGAATGGAC GCAAGAGAGC CAAGGAAATC 
TTCAAAATTA TCAATAAGCC TGACGGCAGA GTGCGCGCTA GTAGCGAAGC GAGAAGCTAT 
GAGCAAAGCG AGAAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Karl ( NM_001298209.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285138.1 Drosophila melanogaster karl, transcript variant D (Karl), mRNA.

Price & Availability↑

CloneID	OFa01320
Clone ID Related Accession (Same CDS sequence)	NM_132520.4 , NM_167303.3 , NM_001298208.1 , NM_001298209.1
Accession Version	NM_001298209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	karl, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGGA TCATGGGCAG GAATGGATCG CTCCTACTGA CCGCCGTCCT CATAATTATC 
CTGGATGTTT TGCTTGCACC AGTGGGTGCC AACCTGTGGA CACGACACAA TAGCAATGCC 
TATCAAACCA GGAGGCGCTC AGGTCCCAGC AATCGGTGTC CCAAGGTGGG AGCCATTAAG 
AACTTCGATT TGGAGCGCAT GATGGGCTGT TGGCACGTGG TTCAGTATTA CGCATCCACA 
GAGGAACTTC CGGAGTACGC GTGCATGCGC AGTCACTTTT CCTTTTCCAA AGAGGATCAG 
CATATAACGA TGAACTTTAG CTACATATTT GCGGAGGATC CGCTGCGCGA GAAGCTCGTG 
GGCAACATCA CCTGGATGAT ACCCAAATTC CAAGAGCCAG GACATTGGCA GCACACGGAG 
GACATATATG AGGGCATCTA CAACACATAC GTTCTGGACA CGGACTACGA CACCTGGGGC 
CTGGTGATGC ACTGCGCCGA GAAGAAGAAG CAACCACGTT ACCTGTCCGC CCTGCTTTTG 
TCGCGGAAAA CATCGTTGGC CGACAACGAG ATCAGCTTTC TGCGCGGCAA GTTGCCGCAG 
GATATCGACA CATCCTTTAT GTTTAACATC GGGCAGGAAT CGTGCGACAA TCTAATGGAA 
TCTAGTCGCG ACGATCCACT GGCCTATGTT GTGAATGGAC GCAAGAGAGC CAAGGAAATC 
TTCAAAATTA TCAATAAGCC TGACGGCAGA GTGCGCGCTA GTAGCGAAGC GAGAAGCTAT 
GAGCAAAGCG AGAAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285138.1
CDS	452..1252
Translation	MERIMGRNGSLLLTAVLIIILDVLLAPVGANLWTRHNSNAYQTRRRSGPSNRCPKVGAIKNFDLERMMGCWHVVQYYASTEELPEYACMRSHFSFSKEDQHITMNFSYIFAEDPLREKLVGNITWMIPKFQEPGHWQHTEDIYEGIYNTYVLDTDYDTWGLVMHCAEKKKQPRYLSALLLSRKTSLADNEISFLRGKLPQDIDTSFMFNIGQESCDNLMESSRDDPLAYVVNGRKRAKEIFKIINKPDGRVRASSEARSYEQSEKL

Target ORF information:

RefSeq Version	NM_001298209.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster karl, transcript variant D (Karl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298209.1

ORF Insert Sequence:

ATGGAGAGGA TCATGGGCAG GAATGGATCG CTCCTACTGA CCGCCGTCCT CATAATTATC 
CTGGATGTTT TGCTTGCACC AGTGGGTGCC AACCTGTGGA CACGACACAA TAGCAATGCC 
TATCAAACCA GGAGGCGCTC AGGTCCCAGC AATCGGTGTC CCAAGGTGGG AGCCATTAAG 
AACTTCGATT TGGAGCGCAT GATGGGCTGT TGGCACGTGG TTCAGTATTA CGCATCCACA 
GAGGAACTTC CGGAGTACGC GTGCATGCGC AGTCACTTTT CCTTTTCCAA AGAGGATCAG 
CATATAACGA TGAACTTTAG CTACATATTT GCGGAGGATC CGCTGCGCGA GAAGCTCGTG 
GGCAACATCA CCTGGATGAT ACCCAAATTC CAAGAGCCAG GACATTGGCA GCACACGGAG 
GACATATATG AGGGCATCTA CAACACATAC GTTCTGGACA CGGACTACGA CACCTGGGGC 
CTGGTGATGC ACTGCGCCGA GAAGAAGAAG CAACCACGTT ACCTGTCCGC CCTGCTTTTG 
TCGCGGAAAA CATCGTTGGC CGACAACGAG ATCAGCTTTC TGCGCGGCAA GTTGCCGCAG 
GATATCGACA CATCCTTTAT GTTTAACATC GGGCAGGAAT CGTGCGACAA TCTAATGGAA 
TCTAGTCGCG ACGATCCACT GGCCTATGTT GTGAATGGAC GCAAGAGAGC CAAGGAAATC 
TTCAAAATTA TCAATAAGCC TGACGGCAGA GTGCGCGCTA GTAGCGAAGC GAGAAGCTAT 
GAGCAAAGCG AGAAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Karl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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