Ptp10D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptp10D
Entrez Gene ID	32115
Full Name	Protein tyrosine phosphatase 10D
Synonyms	10D,CG1817,CT4920,DPTP,DPTP10D,DPTP[[10D]],Dmel\CG1817,PTP10D,Ptp10,R-PTP 10D,dptp,ptp10D,ptp10d
General protein information	Preferred Names Protein tyrosine phosphatase 10D Names CG1817 gene product from transcript CG1817-RG CG1817-PB CG1817-PE CG1817-PF CG1817-PG CG1817-PH CG1817-PI Ptp10D-PB Ptp10D-PE Ptp10D-PF Ptp10D-PG Ptp10D-PH Ptp10D-PI protein tyrosine phosphatase 10B protein tyrosine phosphatase 10D
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10D1-10D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_167292.3	NP_727544.2	protein tyrosine phosphatase 10D, isoform B
NM_206690.2	NP_996413.2	protein tyrosine phosphatase 10D, isoform F
NM_001272524.1	NP_001259453.1	protein tyrosine phosphatase 10D, isoform H
NM_206691.3	NP_996414.2	protein tyrosine phosphatase 10D, isoform G
NM_001272525.1	NP_001259454.1	protein tyrosine phosphatase 10D, isoform I
NM_001144715.2	NP_001138187.1	protein tyrosine phosphatase 10D, isoform E

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PTPRB	XP_538283.3
Rattus norvegicus (Norway rat)	Ptprb	XP_003750369.1
Drosophila melanogaster (fruit fly)	Ptp10D	NP_001138187.1
Homo sapiens (human)	PTPRB	NP_001103224.1
Pan troglodytes (chimpanzee)	PTPRB	XP_001156918.2
Mus musculus (house mouse)	Ptprb	NP_084204.2
Gallus gallus (chicken)	PTPRB	XP_416095.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004246	XP_003436753.1
	PTPRB	XP_001117453.1
Bos taurus (cattle)	PTPRB	NP_001192226.1
Danio rerio (zebrafish)	ptprb	NP_001186063.1
Xenopus tropicalis (tropical clawed frog)	ptprb	NP_001131088.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

through a genetic screen in Drosophila, we identify the ligand Sas and the receptor-type tyrosine phosphatase PTP10D as the cell-surface ligand-receptor system that drives tumour-suppressive cell competition
Title: The ligand Sas and its receptor PTP10D drive tumour-suppressive cell competition.

The study of this study showed that neuronal Ptp10D restrains signaling by overexpressed glial Sas, which would otherwise produce strong glial and axonal phenotypes.
Title: Interactions between a receptor tyrosine phosphatase and a cell surface ligand regulate axon guidance and glial-neuronal communication.

Described is the crystal structures of Drosophila melanogaster PTP10D in the apo form as well as in a complex with a substrate peptide and an inhibitor.
Title: Conformational basis for substrate recruitment in protein tyrosine phosphatase 10D.

Results describe a unique tracheal tubulogenesis phenotype caused by loss of both Drosophila type III receptor tyrosine phosphatases (RPTPs), Ptp4E and Ptp10D.
Title: Receptor tyrosine phosphatases control tracheal tube geometries through negative regulation of Egfr signaling.

Ptp10D is expressed only on the innermost core fibers during development of the larval mushroom body.
Title: Receptor tyrosine phosphatases regulate birth order-dependent axonal fasciculation and midline repulsion during development of the Drosophila mushroom body.

The following Ptp10D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptp10D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01302	NM_206690.2 Latest version!	Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant F (Ptp10D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa01302	NM_206691.3 Latest version!	Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant G (Ptp10D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa01303	NM_001272525.1 Latest version!	Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant I (Ptp10D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa01303	NM_001144715.2 Latest version!	Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant E (Ptp10D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa01304	NM_167292.3 Latest version!	Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant B (Ptp10D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa02789	NM_001272524.1 Latest version!	Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant H (Ptp10D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptp10D ( NM_206690.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996413.2 Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant F (Ptp10D), mRNA.

Price & Availability↑

CloneID	OFa01302
Clone ID Related Accession (Same CDS sequence)	NM_206690.2 , NM_206691.3
Accession Version	NM_206690.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein tyrosine phosphatase 10D, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_206690.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGTACC AGCTAAGTAA AGCCACTACT AGAATCCGGC TCAAAAGGCA AAAAGCCGTT 
CCACAACATC GCTGGCTCTG GAGTTTAGCA TTTCTGGCAG CATTTACACT AAAGGACGTT 
CGATGTGCAG ATCTAGCCAT AAGTATACCC AATAATCCCG GCCTGGACGA TGGGGCCTCC 
TATCGACTGG ACTATAGTCC GCCCTTCGGT TATCCGGAGC CCAACACGAC GATTGCCTCG 
CGGGAAATCG GCGATGAGAT TCAATTCTCA CGCGCCCTAC CGGGCACCAA ATACAACTTT 
TGGCTGTACT ACACGAACTT TACGCACCAC GATTGGCTCA CCTGGACGGT GACGATAACA 
ACGGCTCCCG ATCCGCCGTC GAATCTGTCC GTTCAGGTGA GAAGCGGCAA GAATGCCATC 
ATCCTATGGT CACCGCCCAC CCAGGGCAGC TATACGGCGT TTAAGATCAA GGTGCTCGGA 
CTGTCGGAGG CGTCGAGCAG CTACAACCGC ACCTTTCAGG TGAACGACAA TACATTCCAG 
CACAGCGTCA AGGAGCTGAC ACCCGGAGCC ACCTACCAGG TGCAGGCATA TACCATCTAC 
GATGGCAAGG AGTCGGTGGC CTACACTAGT CGCAACTTCA CCACAAAGCC CAACACTCCG 
GGGAAATTCA TCGTCTGGTT CCGTAATGAG ACGACACTGC TGGTCCTGTG GCAGCCGCCA 
TATCCGGCGG GCATCTACAC GCACTACAAG GTATCCATCG AGCCGCCGGA TGCCAACGAT 
AGTGTGCTCT ATGTGGAAAA GGAGGGCGAA CCGCCCGGAC CGGCGCAGGC TGCCTTCAAG 
GGTCTGGTGC CCGGAAGGGC GTACAACATA TCCGTTCAGA CGATGTCCGA GGATGAGATC 
TCATTGCCGA CGACGGCGCA ATATCGAACG GTGCCGTTGC GTCCGCTGAA CGTGACCTTT 
GACCGTGACT TTATTACCTC CAATTCGTTC CGTGTCCTGT GGGAGGCGCC CAAGGGGATA 
TCCGAATTTG ACAAATACCA GGTATCGGTG GCCACAACAC GACGCCAATC CACAGTGCCG 
CGCAGCAATG AACCGGTGGC ATTCTTTGAT TTTCGCGACA TCGCCGAACC GGGCAAGACG 
TTCAATGTGA TCGTGAAGAC CGTATCCGGC AAGGTTACCT CGTGGCCAGC CACCGGGGAT 
GTGACACTGC GACCACTGCC CGTTCGCAAT TTGCGGAGCA TCAACGATGA CAAGACGAAT 
ACTATGATCA TAACGTGGGA AGCGGATCCG GCCAGCACGC AGGATGAGTA TCGCATTGTA 
TACCACGAAC TGGAGACATT TAATGGTGAC ACCAGTACCC TGACCACGGA TCGGACTCGA 
TTCACACTGG AGAGCCTGCT ACCCGGTCGC AACTACTCAT TGTCCGTCCA GGCGGTATCC 
AAGAAGATGG AATCGAATGA GACTAGCATC TTTGTGGTCA CCCGACCCTC GTCGCCCATC 
ATCGAGGACT TGAAGAGCAT ACGGATGGGT CTGAACATCA GTTGGAAGAG CGATGTCAAC 
TCCAAGCAGG AGCAGTACGA GGTGTTGTAC TCGCGCAACG GAACCAGCGA TTTGCGAACC 
CAAAAGACCA AAGAGTCGCG TCTGGTGATC AAGAATCTGC AGCCAGGTGC TGGCTATGAA 
CTCAAGGTGT TTGCAGTTAG TCACGATTTG CGCAGCGAAC CACATGCCTA TTTCCAAGCA 
GTTTATCCCA ATCCACCACG CAACATGACC ATCGAAACGG TGAGGAGTAA CTCGGTGCTG 
GTACACTGGT CACCGCCGGA AAGCGGTGAA TTTACCGAGT ACTCGATACG CTATCGCACG 
GACAGCGAAC AGCAGTGGGT GCGATTGCCC AGCGTTCGGT CCACGGAGGC GGATATCACC 
GATATGACCA AGGGCGAAAA GTACACCATC CAGGTGAACA CGGTCAGCTT TGGCGTAGAG 
AGTCCAGTGC CCCAGGAGGT GAACACGACG GTGCCGCCGA ATCCGGTGTC CAATATCATT 
CAACTGGTGG ACTCACGGAA CATTACGTTG GAGTGGCCCA AGCCAGAGGG TCGCGTGGAA 
TCGTACATTC TTAAGTGGTG GCCCAGCGAT AATCCCGGTC GTGTCCAGAC CAAGAATGTC 
TCCGAGAACA AGTCGGCCGA CGATTTGTCA ACAGTGCGTG TCCTCATCGG CGAACTGATG 
CCCGGCGTGC AGTACAAGTT TGACATCCAG ACGACGTCGT ATGGAATCCT GTCGGGCATC 
ACCAGTCTGT ATCCGCGCAC CATGCCGCTC ATCCAGTCGG ACGTGGTGGT GGCCAATGGC 
GAGAAGGAGG ACGAGAGGGA CACCATCACT CTGAGCTACA CACCCACACC GCAGTCGTCG 
TCCAAGTTCG ATATCTATCG ATTCTCTCTC GGCGATGCCG AGATCCGGGA CAAGGAGAAG 
CTGGCCAACG ATACGGATCG CAAGGTGACG TTTACGGGTC TGGTGCCTGG TCGATTGTAC 
AACATCACAG TTTGGACTGT GAGCGGTGGT GTGGCCAGTT TGCCCATACA ACGACAGGAT 
CGCCTGTATC CGGAACCCAT CACACAGCTG CATGCCACCA ACATCACGGA TACGGAGATC 
TCATTGCGCT GGGATTTGCC CAAGGGCGAG TACAATGACT TCGATATTGC CTATCTCACG 
GCGGACAATC TATTGGCCCA GAATATGACC ACCAGGAATG AGATCACCAT CAGTGACCTG 
CGACCCCACA GGAACTACAC CTTCACCGTG GTGGTACGTT CCGGCACTGA ATCGTCCGTG 
CTGAGGAGCA GTTCACCATT ATCCGCTAGC TTTACGACCA ATGAAGCGGT GCCCGGGCGA 
GTTGAACGCT TCCATCCCAC GGATGTTCAG CCCAGCGAGA TCAATTTCGA GTGGTCGCTG 
CCATCGAGTG AGGCAAATGG CGTTATTCGC CAGTTCTCGA TAGCCTATAC GAATATCAAT 
AATCTCACGG ACGCAGGCAT GCAGGACTTT GAGTCGGAGG AGGCATTCGG TGTGATCAAG 
AATCTAAAGC CCGGCGAGAC CTATGTGTTC AAGATTCAGG CCAAGACTGC CATCGGTTTC 
GGTCCGGAGC GGGAGTACCG TCAAACGATG CCGATATTAG CGCCACCACG TCCTGCCACC 
CAAGTGGTGC CCACCGAGGT CTATCGCAGC TCATCGACCA TCCAGATTCG GTTTAGGAAG 
AACTACTTCT CGGATCAAAA CGGCCAGGTG CGCATGTACA CGATCATCGT GGCCGAGGAT 
GATGCCAAGA ATGCATCCGG CCTGGAGATG CCCAGCTGGC TGGATGTGCA GTCGTACAGC 
GTTTGGTTGC CCTATCAGGC CATAGATCCG TACTATCCAT TCGAGAATCG ATCCGTAGAG 
GACTTCACCA TCGGTACGGA GAACTGTGAC AACCACAAGA TCGGCTACTG CAACGGACCA 
CTGAAATCGG GAACCACCTA TCGGGTTAAG GTGCGGGCGT TCACCGGAGC GGATAAGTTC 
ACGGATACCG CCTACAGTTT TCCCATTCAG ACAGATCAAG ACAACACCTC ACTGATTGTG 
GCCATTACGG TGCCGTTAAC TATCATCTTG GTGCTCCTGG TGACACTTTT GTTCTACAAA 
CGACGTCGCA ACAATTGCCG TAAGACGACC AAGGATTCGA GGGCCAACGA CAATATGTCC 
CTGCCGGATA GCGTAATCGA GCAGAATCGC CCCATTCTGA TCAAGAACTT TGCCGAGCAC 
TATCGCCTAA TGTCCGCCGA TTCGGACTTC CGTTTCAGCG AGGAATTCGA GGAACTGAAG 
CACGTTGGCC GGGATCAGCC GTGCACTTTT GCCGATCTAC CCTGCAATCG TCCGAAAAAC 
AGGTTCACCA ATATACTGCC CTACGATCAC TCACGTTTCA AGCTTCAGCC GGTGGACGAT 
GATGAGGGTA GTGATTATAT CAATGCCAAT TACGTGCCGG GTCACAATTC ACCGCGCGAG 
TTCATCGTGA CCCAGGGACC ATTGCATTCG ACACGCGATG ACTTCTGGCG AATGTGCTGG 
GAGAGCAACT CGCGGGCCAT AGTCATGCTG ACCAGGTGCT TTGAGAAGGG GCGCGAGAAG 
TGCGACCAGT ATTGGCCAAA TGATACGGTG CCCGTCTTCT ACGGTGACAT CAAGGTGCAG 
ATACTCAACG ACAGTCACTA TGCCGACTGG GTGATGACCG AGTTCATGCT ATGCAGAGGC 
AGCGAACAGC GCATCCTGCG ACACTTCCAC TTCACCACCT GGCCGGACTT CGGTGTTCCC 
AATCCGCCAC AGACACTGGT GCGCTTTGTG CGCGCATTCC GCGATCGAAT TGGTGCGGAA 
CAGCGACCCA TTGTGGTCCA TTGTAGCGCC GGTGTGGGAA GGTCGGGCAC CTTCATCACC 
CTGGATCGCA TCCTGCAACA GATCAACACG TCTGACTATG TGGACATATT TGGCATAGTA 
TATGCCATGC GCAAGGAGCG CGTTTGGATG GTGCAGACGG AGCAGCAGTA TATCTGCATC 
CACCAGTGCC TGCTGGCGGT GCTCGAGGGG AAGGAGAACA TCGTGGGTCC CGCTCGCGAG 
ATGCACGACA ACGAGGGCTA TGAAGGCCAA CAAGTGCAGC TGGACGAGAA CGGTGATGTG 
GTGGCCACCA TTGAGGGTCA CCTGTCGCAT CATGATCTGC AGCAGGCCGA GGCGGAGGCC 
ATTGACGATG AGAATGCGGC GATACTCCAT GATGACCAGC AGCCGCTGAC CAGTAGCTTT 
ACTGGACACC ACACTCACAT GCCGCCCACC ACATCGATGA GCTCATTTGG CGGCGGAGGT 
GGAGGCCATA CAAATGTGGA TGCACCGGAT AGATGACATT CGGTTGTCAA CCAGAGTGAT 
AACAACAATT CGGTGGTTAT CGTCCTGGTT GACAACAAGC CCAGCTCGAT GATCTGCAAG 
GATAGCAAGG GCGGCAACAT CGATGTGCTC GAATCGCAGC AGCAGCAGCA GCAGCAACAG 
CAACAGCAGC CCAACCAGGG AGGCCACAAC ATAACCACCA TATCCGCCAT CAACGGCTAC 
AATACGCTAC AGCATAGACG GAAATCCCAG CTGATAACGT TCAGCTCGTC ATCCTGTGAT 
ATCAAGAACA GTCTTAGTCA TGAGTACATC AATGGATCCA ATGGATCGGC AGCTAATGGT 
CCACCCAGTT CCGGTTCTGG TTCCGGTTCC GGTCCAGGCA GCAATCGGGC CAGTCGCGCC 
AATGTCCGAT TAAGTTTCGC CGAGGAAGAT GTAATGATCT TGCCACAAAA CCACAGTCAG 
CAATCCAATC ATCAAGATGA CGAGGTCTTC ACGCGACGTC GTTCGCTTTT GGAAGTGGAA 
ATCGGTGTGG AGGTAGGCGA AGATGGTGAA CTTGCACCCC ACGAAATGGA AGAGGATCTC 
GAAGAGGAGG ACGAGGATGA GGAGCTGTAT ATGCACGATG AGTTCGAGAC CCACATCGAT 
ACCAAGTCGA ATAATGCCAA CGATGACAGC GGTGGCGGCA GCTACGAGGA CTCCCATGCC 
CTGCACTCCT CGCTGGGGGG CAGTAATCGA AATAGTCTGG AAAAGGATGA CGACGACATC 
GAGGTGGATG TGATCAGCAC AGACGTTAGC TGTTACGATC AACTGCTCGG CAGCAGTTGC 
AACACGCGGA ACGGCGATGA TGACGACATA GCCACGCTGG TCGGCGATGG CGACTATTCC 
ACCACGAAAC TCAGCAAGGC ATCCAGATTA TCCGGCGCTG GAGTCGGCGG ACTAGTGGTC 
AGCGGGGGAG GAGGTGGAAC TGCAATCGGC GGTGGAATCG CTGTTAACGG TGGTGGTGTT 
TTGGGAAATG GTGTTGGTTC CGAGGCTGGC GGCGGCATCA TCTATGCCAA TCCGTTTATG 
GATGACGAGG GCATTGCAGA GTCGGGCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996413.2
CDS	522..6494
Translation	MLYQLSKATTRIRLKRQKAVPQHRWLWSLAFLAAFTLKDVRCADLAISIPNNPGLDDGASYRLDYSPPFGYPEPNTTIASREIGDEIQFSRALPGTKYNFWLYYTNFTHHDWLTWTVTITTAPDPPSNLSVQVRSGKNAIILWSPPTQGSYTAFKIKVLGLSEASSSYNRTFQVNDNTFQHSVKELTPGATYQVQAYTIYDGKESVAYTSRNFTTKPNTPGKFIVWFRNETTLLVLWQPPYPAGIYTHYKVSIEPPDANDSVLYVEKEGEPPGPAQAAFKGLVPGRAYNISVQTMSEDEISLPTTAQYRTVPLRPLNVTFDRDFITSNSFRVLWEAPKGISEFDKYQVSVATTRRQSTVPRSNEPVAFFDFRDIAEPGKTFNVIVKTVSGKVTSWPATGDVTLRPLPVRNLRSINDDKTNTMIITWEADPASTQDEYRIVYHELETFNGDTSTLTTDRTRFTLESLLPGRNYSLSVQAVSKKMESNETSIFVVTRPSSPIIEDLKSIRMGLNISWKSDVNSKQEQYEVLYSRNGTSDLRTQKTKESRLVIKNLQPGAGYELKVFAVSHDLRSEPHAYFQAVYPNPPRNMTIETVRSNSVLVHWSPPESGEFTEYSIRYRTDSEQQWVRLPSVRSTEADITDMTKGEKYTIQVNTVSFGVESPVPQEVNTTVPPNPVSNIIQLVDSRNITLEWPKPEGRVESYILKWWPSDNPGRVQTKNVSENKSADDLSTVRVLIGELMPGVQYKFDIQTTSYGILSGITSLYPRTMPLIQSDVVVANGEKEDERDTITLSYTPTPQSSSKFDIYRFSLGDAEIRDKEKLANDTDRKVTFTGLVPGRLYNITVWTVSGGVASLPIQRQDRLYPEPITQLHATNITDTEISLRWDLPKGEYNDFDIAYLTADNLLAQNMTTRNEITISDLRPHRNYTFTVVVRSGTESSVLRSSSPLSASFTTNEAVPGRVERFHPTDVQPSEINFEWSLPSSEANGVIRQFSIAYTNINNLTDAGMQDFESEEAFGVIKNLKPGETYVFKIQAKTAIGFGPEREYRQTMPILAPPRPATQVVPTEVYRSSSTIQIRFRKNYFSDQNGQVRMYTIIVAEDDAKNASGLEMPSWLDVQSYSVWLPYQAIDPYYPFENRSVEDFTIGTENCDNHKIGYCNGPLKSGTTYRVKVRAFTGADKFTDTAYSFPIQTDQDNTSLIVAITVPLTIILVLLVTLLFYKRRRNNCRKTTKDSRANDNMSLPDSVIEQNRPILIKNFAEHYRLMSADSDFRFSEEFEELKHVGRDQPCTFADLPCNRPKNRFTNILPYDHSRFKLQPVDDDEGSDYINANYVPGHNSPREFIVTQGPLHSTRDDFWRMCWESNSRAIVMLTRCFEKGREKCDQYWPNDTVPVFYGDIKVQILNDSHYADWVMTEFMLCRGSEQRILRHFHFTTWPDFGVPNPPQTLVRFVRAFRDRIGAEQRPIVVHCSAGVGRSGTFITLDRILQQINTSDYVDIFGIVYAMRKERVWMVQTEQQYICIHQCLLAVLEGKENIVGPAREMHDNEGYEGQQVQLDENGDVVATIEGHLSHHDLQQAEAEAIDDENAAILHDDQQPLTSSFTGHHTHMPPTTSMSSFGGGGGGHTNVDAPDR*HSVVNQSDNNNSVVIVLVDNKPSSMICKDSKGGNIDVLESQQQQQQQQQQQPNQGGHNITTISAINGYNTLQHRRKSQLITFSSSSCDIKNSLSHEYINGSNGSAANGPPSSGSGSGSGPGSNRASRANVRLSFAEEDVMILPQNHSQQSNHQDDEVFTRRRSLLEVEIGVEVGEDGELAPHEMEEDLEEEDEDEELYMHDEFETHIDTKSNNANDDSGGGSYEDSHALHSSLGGSNRNSLEKDDDDIEVDVISTDVSCYDQLLGSSCNTRNGDDDDIATLVGDGDYSTTKLSKASRLSGAGVGGLVVSGGGGGTAIGGGIAVNGGGVLGNGVGSEAGGGIIYANPFMDDEGIAESGM

Target ORF information:

RefSeq Version	NM_206690.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant F (Ptp10D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206690.2

ORF Insert Sequence:

ATGTTGTACC AGCTAAGTAA AGCCACTACT AGAATCCGGC TCAAAAGGCA AAAAGCCGTT 
CCACAACATC GCTGGCTCTG GAGTTTAGCA TTTCTGGCAG CATTTACACT AAAGGACGTT 
CGATGTGCAG ATCTAGCCAT AAGTATACCC AATAATCCCG GCCTGGACGA TGGGGCCTCC 
TATCGACTGG ACTATAGTCC GCCCTTCGGT TATCCGGAGC CCAACACGAC GATTGCCTCG 
CGGGAAATCG GCGATGAGAT TCAATTCTCA CGCGCCCTAC CGGGCACCAA ATACAACTTT 
TGGCTGTACT ACACGAACTT TACGCACCAC GATTGGCTCA CCTGGACGGT GACGATAACA 
ACGGCTCCCG ATCCGCCGTC GAATCTGTCC GTTCAGGTGA GAAGCGGCAA GAATGCCATC 
ATCCTATGGT CACCGCCCAC CCAGGGCAGC TATACGGCGT TTAAGATCAA GGTGCTCGGA 
CTGTCGGAGG CGTCGAGCAG CTACAACCGC ACCTTTCAGG TGAACGACAA TACATTCCAG 
CACAGCGTCA AGGAGCTGAC ACCCGGAGCC ACCTACCAGG TGCAGGCATA TACCATCTAC 
GATGGCAAGG AGTCGGTGGC CTACACTAGT CGCAACTTCA CCACAAAGCC CAACACTCCG 
GGGAAATTCA TCGTCTGGTT CCGTAATGAG ACGACACTGC TGGTCCTGTG GCAGCCGCCA 
TATCCGGCGG GCATCTACAC GCACTACAAG GTATCCATCG AGCCGCCGGA TGCCAACGAT 
AGTGTGCTCT ATGTGGAAAA GGAGGGCGAA CCGCCCGGAC CGGCGCAGGC TGCCTTCAAG 
GGTCTGGTGC CCGGAAGGGC GTACAACATA TCCGTTCAGA CGATGTCCGA GGATGAGATC 
TCATTGCCGA CGACGGCGCA ATATCGAACG GTGCCGTTGC GTCCGCTGAA CGTGACCTTT 
GACCGTGACT TTATTACCTC CAATTCGTTC CGTGTCCTGT GGGAGGCGCC CAAGGGGATA 
TCCGAATTTG ACAAATACCA GGTATCGGTG GCCACAACAC GACGCCAATC CACAGTGCCG 
CGCAGCAATG AACCGGTGGC ATTCTTTGAT TTTCGCGACA TCGCCGAACC GGGCAAGACG 
TTCAATGTGA TCGTGAAGAC CGTATCCGGC AAGGTTACCT CGTGGCCAGC CACCGGGGAT 
GTGACACTGC GACCACTGCC CGTTCGCAAT TTGCGGAGCA TCAACGATGA CAAGACGAAT 
ACTATGATCA TAACGTGGGA AGCGGATCCG GCCAGCACGC AGGATGAGTA TCGCATTGTA 
TACCACGAAC TGGAGACATT TAATGGTGAC ACCAGTACCC TGACCACGGA TCGGACTCGA 
TTCACACTGG AGAGCCTGCT ACCCGGTCGC AACTACTCAT TGTCCGTCCA GGCGGTATCC 
AAGAAGATGG AATCGAATGA GACTAGCATC TTTGTGGTCA CCCGACCCTC GTCGCCCATC 
ATCGAGGACT TGAAGAGCAT ACGGATGGGT CTGAACATCA GTTGGAAGAG CGATGTCAAC 
TCCAAGCAGG AGCAGTACGA GGTGTTGTAC TCGCGCAACG GAACCAGCGA TTTGCGAACC 
CAAAAGACCA AAGAGTCGCG TCTGGTGATC AAGAATCTGC AGCCAGGTGC TGGCTATGAA 
CTCAAGGTGT TTGCAGTTAG TCACGATTTG CGCAGCGAAC CACATGCCTA TTTCCAAGCA 
GTTTATCCCA ATCCACCACG CAACATGACC ATCGAAACGG TGAGGAGTAA CTCGGTGCTG 
GTACACTGGT CACCGCCGGA AAGCGGTGAA TTTACCGAGT ACTCGATACG CTATCGCACG 
GACAGCGAAC AGCAGTGGGT GCGATTGCCC AGCGTTCGGT CCACGGAGGC GGATATCACC 
GATATGACCA AGGGCGAAAA GTACACCATC CAGGTGAACA CGGTCAGCTT TGGCGTAGAG 
AGTCCAGTGC CCCAGGAGGT GAACACGACG GTGCCGCCGA ATCCGGTGTC CAATATCATT 
CAACTGGTGG ACTCACGGAA CATTACGTTG GAGTGGCCCA AGCCAGAGGG TCGCGTGGAA 
TCGTACATTC TTAAGTGGTG GCCCAGCGAT AATCCCGGTC GTGTCCAGAC CAAGAATGTC 
TCCGAGAACA AGTCGGCCGA CGATTTGTCA ACAGTGCGTG TCCTCATCGG CGAACTGATG 
CCCGGCGTGC AGTACAAGTT TGACATCCAG ACGACGTCGT ATGGAATCCT GTCGGGCATC 
ACCAGTCTGT ATCCGCGCAC CATGCCGCTC ATCCAGTCGG ACGTGGTGGT GGCCAATGGC 
GAGAAGGAGG ACGAGAGGGA CACCATCACT CTGAGCTACA CACCCACACC GCAGTCGTCG 
TCCAAGTTCG ATATCTATCG ATTCTCTCTC GGCGATGCCG AGATCCGGGA CAAGGAGAAG 
CTGGCCAACG ATACGGATCG CAAGGTGACG TTTACGGGTC TGGTGCCTGG TCGATTGTAC 
AACATCACAG TTTGGACTGT GAGCGGTGGT GTGGCCAGTT TGCCCATACA ACGACAGGAT 
CGCCTGTATC CGGAACCCAT CACACAGCTG CATGCCACCA ACATCACGGA TACGGAGATC 
TCATTGCGCT GGGATTTGCC CAAGGGCGAG TACAATGACT TCGATATTGC CTATCTCACG 
GCGGACAATC TATTGGCCCA GAATATGACC ACCAGGAATG AGATCACCAT CAGTGACCTG 
CGACCCCACA GGAACTACAC CTTCACCGTG GTGGTACGTT CCGGCACTGA ATCGTCCGTG 
CTGAGGAGCA GTTCACCATT ATCCGCTAGC TTTACGACCA ATGAAGCGGT GCCCGGGCGA 
GTTGAACGCT TCCATCCCAC GGATGTTCAG CCCAGCGAGA TCAATTTCGA GTGGTCGCTG 
CCATCGAGTG AGGCAAATGG CGTTATTCGC CAGTTCTCGA TAGCCTATAC GAATATCAAT 
AATCTCACGG ACGCAGGCAT GCAGGACTTT GAGTCGGAGG AGGCATTCGG TGTGATCAAG 
AATCTAAAGC CCGGCGAGAC CTATGTGTTC AAGATTCAGG CCAAGACTGC CATCGGTTTC 
GGTCCGGAGC GGGAGTACCG TCAAACGATG CCGATATTAG CGCCACCACG TCCTGCCACC 
CAAGTGGTGC CCACCGAGGT CTATCGCAGC TCATCGACCA TCCAGATTCG GTTTAGGAAG 
AACTACTTCT CGGATCAAAA CGGCCAGGTG CGCATGTACA CGATCATCGT GGCCGAGGAT 
GATGCCAAGA ATGCATCCGG CCTGGAGATG CCCAGCTGGC TGGATGTGCA GTCGTACAGC 
GTTTGGTTGC CCTATCAGGC CATAGATCCG TACTATCCAT TCGAGAATCG ATCCGTAGAG 
GACTTCACCA TCGGTACGGA GAACTGTGAC AACCACAAGA TCGGCTACTG CAACGGACCA 
CTGAAATCGG GAACCACCTA TCGGGTTAAG GTGCGGGCGT TCACCGGAGC GGATAAGTTC 
ACGGATACCG CCTACAGTTT TCCCATTCAG ACAGATCAAG ACAACACCTC ACTGATTGTG 
GCCATTACGG TGCCGTTAAC TATCATCTTG GTGCTCCTGG TGACACTTTT GTTCTACAAA 
CGACGTCGCA ACAATTGCCG TAAGACGACC AAGGATTCGA GGGCCAACGA CAATATGTCC 
CTGCCGGATA GCGTAATCGA GCAGAATCGC CCCATTCTGA TCAAGAACTT TGCCGAGCAC 
TATCGCCTAA TGTCCGCCGA TTCGGACTTC CGTTTCAGCG AGGAATTCGA GGAACTGAAG 
CACGTTGGCC GGGATCAGCC GTGCACTTTT GCCGATCTAC CCTGCAATCG TCCGAAAAAC 
AGGTTCACCA ATATACTGCC CTACGATCAC TCACGTTTCA AGCTTCAGCC GGTGGACGAT 
GATGAGGGTA GTGATTATAT CAATGCCAAT TACGTGCCGG GTCACAATTC ACCGCGCGAG 
TTCATCGTGA CCCAGGGACC ATTGCATTCG ACACGCGATG ACTTCTGGCG AATGTGCTGG 
GAGAGCAACT CGCGGGCCAT AGTCATGCTG ACCAGGTGCT TTGAGAAGGG GCGCGAGAAG 
TGCGACCAGT ATTGGCCAAA TGATACGGTG CCCGTCTTCT ACGGTGACAT CAAGGTGCAG 
ATACTCAACG ACAGTCACTA TGCCGACTGG GTGATGACCG AGTTCATGCT ATGCAGAGGC 
AGCGAACAGC GCATCCTGCG ACACTTCCAC TTCACCACCT GGCCGGACTT CGGTGTTCCC 
AATCCGCCAC AGACACTGGT GCGCTTTGTG CGCGCATTCC GCGATCGAAT TGGTGCGGAA 
CAGCGACCCA TTGTGGTCCA TTGTAGCGCC GGTGTGGGAA GGTCGGGCAC CTTCATCACC 
CTGGATCGCA TCCTGCAACA GATCAACACG TCTGACTATG TGGACATATT TGGCATAGTA 
TATGCCATGC GCAAGGAGCG CGTTTGGATG GTGCAGACGG AGCAGCAGTA TATCTGCATC 
CACCAGTGCC TGCTGGCGGT GCTCGAGGGG AAGGAGAACA TCGTGGGTCC CGCTCGCGAG 
ATGCACGACA ACGAGGGCTA TGAAGGCCAA CAAGTGCAGC TGGACGAGAA CGGTGATGTG 
GTGGCCACCA TTGAGGGTCA CCTGTCGCAT CATGATCTGC AGCAGGCCGA GGCGGAGGCC 
ATTGACGATG AGAATGCGGC GATACTCCAT GATGACCAGC AGCCGCTGAC CAGTAGCTTT 
ACTGGACACC ACACTCACAT GCCGCCCACC ACATCGATGA GCTCATTTGG CGGCGGAGGT 
GGAGGCCATA CAAATGTGGA TGCACCGGAT AGATGACATT CGGTTGTCAA CCAGAGTGAT 
AACAACAATT CGGTGGTTAT CGTCCTGGTT GACAACAAGC CCAGCTCGAT GATCTGCAAG 
GATAGCAAGG GCGGCAACAT CGATGTGCTC GAATCGCAGC AGCAGCAGCA GCAGCAACAG 
CAACAGCAGC CCAACCAGGG AGGCCACAAC ATAACCACCA TATCCGCCAT CAACGGCTAC 
AATACGCTAC AGCATAGACG GAAATCCCAG CTGATAACGT TCAGCTCGTC ATCCTGTGAT 
ATCAAGAACA GTCTTAGTCA TGAGTACATC AATGGATCCA ATGGATCGGC AGCTAATGGT 
CCACCCAGTT CCGGTTCTGG TTCCGGTTCC GGTCCAGGCA GCAATCGGGC CAGTCGCGCC 
AATGTCCGAT TAAGTTTCGC CGAGGAAGAT GTAATGATCT TGCCACAAAA CCACAGTCAG 
CAATCCAATC ATCAAGATGA CGAGGTCTTC ACGCGACGTC GTTCGCTTTT GGAAGTGGAA 
ATCGGTGTGG AGGTAGGCGA AGATGGTGAA CTTGCACCCC ACGAAATGGA AGAGGATCTC 
GAAGAGGAGG ACGAGGATGA GGAGCTGTAT ATGCACGATG AGTTCGAGAC CCACATCGAT 
ACCAAGTCGA ATAATGCCAA CGATGACAGC GGTGGCGGCA GCTACGAGGA CTCCCATGCC 
CTGCACTCCT CGCTGGGGGG CAGTAATCGA AATAGTCTGG AAAAGGATGA CGACGACATC 
GAGGTGGATG TGATCAGCAC AGACGTTAGC TGTTACGATC AACTGCTCGG CAGCAGTTGC 
AACACGCGGA ACGGCGATGA TGACGACATA GCCACGCTGG TCGGCGATGG CGACTATTCC 
ACCACGAAAC TCAGCAAGGC ATCCAGATTA TCCGGCGCTG GAGTCGGCGG ACTAGTGGTC 
AGCGGGGGAG GAGGTGGAAC TGCAATCGGC GGTGGAATCG CTGTTAACGG TGGTGGTGTT 
TTGGGAAATG GTGTTGGTTC CGAGGCTGGC GGCGGCATCA TCTATGCCAA TCCGTTTATG 
GATGACGAGG GCATTGCAGA GTCGGGCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptp10D ( NM_206691.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996414.2 Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant G (Ptp10D), mRNA.

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CloneID	OFa01302
Clone ID Related Accession (Same CDS sequence)	NM_206690.2 , NM_206691.3
Accession Version	NM_206691.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein tyrosine phosphatase 10D, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_206691.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGTACC AGCTAAGTAA AGCCACTACT AGAATCCGGC TCAAAAGGCA AAAAGCCGTT 
CCACAACATC GCTGGCTCTG GAGTTTAGCA TTTCTGGCAG CATTTACACT AAAGGACGTT 
CGATGTGCAG ATCTAGCCAT AAGTATACCC AATAATCCCG GCCTGGACGA TGGGGCCTCC 
TATCGACTGG ACTATAGTCC GCCCTTCGGT TATCCGGAGC CCAACACGAC GATTGCCTCG 
CGGGAAATCG GCGATGAGAT TCAATTCTCA CGCGCCCTAC CGGGCACCAA ATACAACTTT 
TGGCTGTACT ACACGAACTT TACGCACCAC GATTGGCTCA CCTGGACGGT GACGATAACA 
ACGGCTCCCG ATCCGCCGTC GAATCTGTCC GTTCAGGTGA GAAGCGGCAA GAATGCCATC 
ATCCTATGGT CACCGCCCAC CCAGGGCAGC TATACGGCGT TTAAGATCAA GGTGCTCGGA 
CTGTCGGAGG CGTCGAGCAG CTACAACCGC ACCTTTCAGG TGAACGACAA TACATTCCAG 
CACAGCGTCA AGGAGCTGAC ACCCGGAGCC ACCTACCAGG TGCAGGCATA TACCATCTAC 
GATGGCAAGG AGTCGGTGGC CTACACTAGT CGCAACTTCA CCACAAAGCC CAACACTCCG 
GGGAAATTCA TCGTCTGGTT CCGTAATGAG ACGACACTGC TGGTCCTGTG GCAGCCGCCA 
TATCCGGCGG GCATCTACAC GCACTACAAG GTATCCATCG AGCCGCCGGA TGCCAACGAT 
AGTGTGCTCT ATGTGGAAAA GGAGGGCGAA CCGCCCGGAC CGGCGCAGGC TGCCTTCAAG 
GGTCTGGTGC CCGGAAGGGC GTACAACATA TCCGTTCAGA CGATGTCCGA GGATGAGATC 
TCATTGCCGA CGACGGCGCA ATATCGAACG GTGCCGTTGC GTCCGCTGAA CGTGACCTTT 
GACCGTGACT TTATTACCTC CAATTCGTTC CGTGTCCTGT GGGAGGCGCC CAAGGGGATA 
TCCGAATTTG ACAAATACCA GGTATCGGTG GCCACAACAC GACGCCAATC CACAGTGCCG 
CGCAGCAATG AACCGGTGGC ATTCTTTGAT TTTCGCGACA TCGCCGAACC GGGCAAGACG 
TTCAATGTGA TCGTGAAGAC CGTATCCGGC AAGGTTACCT CGTGGCCAGC CACCGGGGAT 
GTGACACTGC GACCACTGCC CGTTCGCAAT TTGCGGAGCA TCAACGATGA CAAGACGAAT 
ACTATGATCA TAACGTGGGA AGCGGATCCG GCCAGCACGC AGGATGAGTA TCGCATTGTA 
TACCACGAAC TGGAGACATT TAATGGTGAC ACCAGTACCC TGACCACGGA TCGGACTCGA 
TTCACACTGG AGAGCCTGCT ACCCGGTCGC AACTACTCAT TGTCCGTCCA GGCGGTATCC 
AAGAAGATGG AATCGAATGA GACTAGCATC TTTGTGGTCA CCCGACCCTC GTCGCCCATC 
ATCGAGGACT TGAAGAGCAT ACGGATGGGT CTGAACATCA GTTGGAAGAG CGATGTCAAC 
TCCAAGCAGG AGCAGTACGA GGTGTTGTAC TCGCGCAACG GAACCAGCGA TTTGCGAACC 
CAAAAGACCA AAGAGTCGCG TCTGGTGATC AAGAATCTGC AGCCAGGTGC TGGCTATGAA 
CTCAAGGTGT TTGCAGTTAG TCACGATTTG CGCAGCGAAC CACATGCCTA TTTCCAAGCA 
GTTTATCCCA ATCCACCACG CAACATGACC ATCGAAACGG TGAGGAGTAA CTCGGTGCTG 
GTACACTGGT CACCGCCGGA AAGCGGTGAA TTTACCGAGT ACTCGATACG CTATCGCACG 
GACAGCGAAC AGCAGTGGGT GCGATTGCCC AGCGTTCGGT CCACGGAGGC GGATATCACC 
GATATGACCA AGGGCGAAAA GTACACCATC CAGGTGAACA CGGTCAGCTT TGGCGTAGAG 
AGTCCAGTGC CCCAGGAGGT GAACACGACG GTGCCGCCGA ATCCGGTGTC CAATATCATT 
CAACTGGTGG ACTCACGGAA CATTACGTTG GAGTGGCCCA AGCCAGAGGG TCGCGTGGAA 
TCGTACATTC TTAAGTGGTG GCCCAGCGAT AATCCCGGTC GTGTCCAGAC CAAGAATGTC 
TCCGAGAACA AGTCGGCCGA CGATTTGTCA ACAGTGCGTG TCCTCATCGG CGAACTGATG 
CCCGGCGTGC AGTACAAGTT TGACATCCAG ACGACGTCGT ATGGAATCCT GTCGGGCATC 
ACCAGTCTGT ATCCGCGCAC CATGCCGCTC ATCCAGTCGG ACGTGGTGGT GGCCAATGGC 
GAGAAGGAGG ACGAGAGGGA CACCATCACT CTGAGCTACA CACCCACACC GCAGTCGTCG 
TCCAAGTTCG ATATCTATCG ATTCTCTCTC GGCGATGCCG AGATCCGGGA CAAGGAGAAG 
CTGGCCAACG ATACGGATCG CAAGGTGACG TTTACGGGTC TGGTGCCTGG TCGATTGTAC 
AACATCACAG TTTGGACTGT GAGCGGTGGT GTGGCCAGTT TGCCCATACA ACGACAGGAT 
CGCCTGTATC CGGAACCCAT CACACAGCTG CATGCCACCA ACATCACGGA TACGGAGATC 
TCATTGCGCT GGGATTTGCC CAAGGGCGAG TACAATGACT TCGATATTGC CTATCTCACG 
GCGGACAATC TATTGGCCCA GAATATGACC ACCAGGAATG AGATCACCAT CAGTGACCTG 
CGACCCCACA GGAACTACAC CTTCACCGTG GTGGTACGTT CCGGCACTGA ATCGTCCGTG 
CTGAGGAGCA GTTCACCATT ATCCGCTAGC TTTACGACCA ATGAAGCGGT GCCCGGGCGA 
GTTGAACGCT TCCATCCCAC GGATGTTCAG CCCAGCGAGA TCAATTTCGA GTGGTCGCTG 
CCATCGAGTG AGGCAAATGG CGTTATTCGC CAGTTCTCGA TAGCCTATAC GAATATCAAT 
AATCTCACGG ACGCAGGCAT GCAGGACTTT GAGTCGGAGG AGGCATTCGG TGTGATCAAG 
AATCTAAAGC CCGGCGAGAC CTATGTGTTC AAGATTCAGG CCAAGACTGC CATCGGTTTC 
GGTCCGGAGC GGGAGTACCG TCAAACGATG CCGATATTAG CGCCACCACG TCCTGCCACC 
CAAGTGGTGC CCACCGAGGT CTATCGCAGC TCATCGACCA TCCAGATTCG GTTTAGGAAG 
AACTACTTCT CGGATCAAAA CGGCCAGGTG CGCATGTACA CGATCATCGT GGCCGAGGAT 
GATGCCAAGA ATGCATCCGG CCTGGAGATG CCCAGCTGGC TGGATGTGCA GTCGTACAGC 
GTTTGGTTGC CCTATCAGGC CATAGATCCG TACTATCCAT TCGAGAATCG ATCCGTAGAG 
GACTTCACCA TCGGTACGGA GAACTGTGAC AACCACAAGA TCGGCTACTG CAACGGACCA 
CTGAAATCGG GAACCACCTA TCGGGTTAAG GTGCGGGCGT TCACCGGAGC GGATAAGTTC 
ACGGATACCG CCTACAGTTT TCCCATTCAG ACAGATCAAG ACAACACCTC ACTGATTGTG 
GCCATTACGG TGCCGTTAAC TATCATCTTG GTGCTCCTGG TGACACTTTT GTTCTACAAA 
CGACGTCGCA ACAATTGCCG TAAGACGACC AAGGATTCGA GGGCCAACGA CAATATGTCC 
CTGCCGGATA GCGTAATCGA GCAGAATCGC CCCATTCTGA TCAAGAACTT TGCCGAGCAC 
TATCGCCTAA TGTCCGCCGA TTCGGACTTC CGTTTCAGCG AGGAATTCGA GGAACTGAAG 
CACGTTGGCC GGGATCAGCC GTGCACTTTT GCCGATCTAC CCTGCAATCG TCCGAAAAAC 
AGGTTCACCA ATATACTGCC CTACGATCAC TCACGTTTCA AGCTTCAGCC GGTGGACGAT 
GATGAGGGTA GTGATTATAT CAATGCCAAT TACGTGCCGG GTCACAATTC ACCGCGCGAG 
TTCATCGTGA CCCAGGGACC ATTGCATTCG ACACGCGATG ACTTCTGGCG AATGTGCTGG 
GAGAGCAACT CGCGGGCCAT AGTCATGCTG ACCAGGTGCT TTGAGAAGGG GCGCGAGAAG 
TGCGACCAGT ATTGGCCAAA TGATACGGTG CCCGTCTTCT ACGGTGACAT CAAGGTGCAG 
ATACTCAACG ACAGTCACTA TGCCGACTGG GTGATGACCG AGTTCATGCT ATGCAGAGGC 
AGCGAACAGC GCATCCTGCG ACACTTCCAC TTCACCACCT GGCCGGACTT CGGTGTTCCC 
AATCCGCCAC AGACACTGGT GCGCTTTGTG CGCGCATTCC GCGATCGAAT TGGTGCGGAA 
CAGCGACCCA TTGTGGTCCA TTGTAGCGCC GGTGTGGGAA GGTCGGGCAC CTTCATCACC 
CTGGATCGCA TCCTGCAACA GATCAACACG TCTGACTATG TGGACATATT TGGCATAGTA 
TATGCCATGC GCAAGGAGCG CGTTTGGATG GTGCAGACGG AGCAGCAGTA TATCTGCATC 
CACCAGTGCC TGCTGGCGGT GCTCGAGGGG AAGGAGAACA TCGTGGGTCC CGCTCGCGAG 
ATGCACGACA ACGAGGGCTA TGAAGGCCAA CAAGTGCAGC TGGACGAGAA CGGTGATGTG 
GTGGCCACCA TTGAGGGTCA CCTGTCGCAT CATGATCTGC AGCAGGCCGA GGCGGAGGCC 
ATTGACGATG AGAATGCGGC GATACTCCAT GATGACCAGC AGCCGCTGAC CAGTAGCTTT 
ACTGGACACC ACACTCACAT GCCGCCCACC ACATCGATGA GCTCATTTGG CGGCGGAGGT 
GGAGGCCATA CAAATGTGGA TGCACCGGAT AGATGACATT CGGTTGTCAA CCAGAGTGAT 
AACAACAATT CGGTGGTTAT CGTCCTGGTT GACAACAAGC CCAGCTCGAT GATCTGCAAG 
GATAGCAAGG GCGGCAACAT CGATGTGCTC GAATCGCAGC AGCAGCAGCA GCAGCAACAG 
CAACAGCAGC CCAACCAGGG AGGCCACAAC ATAACCACCA TATCCGCCAT CAACGGCTAC 
AATACGCTAC AGCATAGACG GAAATCCCAG CTGATAACGT TCAGCTCGTC ATCCTGTGAT 
ATCAAGAACA GTCTTAGTCA TGAGTACATC AATGGATCCA ATGGATCGGC AGCTAATGGT 
CCACCCAGTT CCGGTTCTGG TTCCGGTTCC GGTCCAGGCA GCAATCGGGC CAGTCGCGCC 
AATGTCCGAT TAAGTTTCGC CGAGGAAGAT GTAATGATCT TGCCACAAAA CCACAGTCAG 
CAATCCAATC ATCAAGATGA CGAGGTCTTC ACGCGACGTC GTTCGCTTTT GGAAGTGGAA 
ATCGGTGTGG AGGTAGGCGA AGATGGTGAA CTTGCACCCC ACGAAATGGA AGAGGATCTC 
GAAGAGGAGG ACGAGGATGA GGAGCTGTAT ATGCACGATG AGTTCGAGAC CCACATCGAT 
ACCAAGTCGA ATAATGCCAA CGATGACAGC GGTGGCGGCA GCTACGAGGA CTCCCATGCC 
CTGCACTCCT CGCTGGGGGG CAGTAATCGA AATAGTCTGG AAAAGGATGA CGACGACATC 
GAGGTGGATG TGATCAGCAC AGACGTTAGC TGTTACGATC AACTGCTCGG CAGCAGTTGC 
AACACGCGGA ACGGCGATGA TGACGACATA GCCACGCTGG TCGGCGATGG CGACTATTCC 
ACCACGAAAC TCAGCAAGGC ATCCAGATTA TCCGGCGCTG GAGTCGGCGG ACTAGTGGTC 
AGCGGGGGAG GAGGTGGAAC TGCAATCGGC GGTGGAATCG CTGTTAACGG TGGTGGTGTT 
TTGGGAAATG GTGTTGGTTC CGAGGCTGGC GGCGGCATCA TCTATGCCAA TCCGTTTATG 
GATGACGAGG GCATTGCAGA GTCGGGCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996414.2
CDS	694..6666
Translation	MLYQLSKATTRIRLKRQKAVPQHRWLWSLAFLAAFTLKDVRCADLAISIPNNPGLDDGASYRLDYSPPFGYPEPNTTIASREIGDEIQFSRALPGTKYNFWLYYTNFTHHDWLTWTVTITTAPDPPSNLSVQVRSGKNAIILWSPPTQGSYTAFKIKVLGLSEASSSYNRTFQVNDNTFQHSVKELTPGATYQVQAYTIYDGKESVAYTSRNFTTKPNTPGKFIVWFRNETTLLVLWQPPYPAGIYTHYKVSIEPPDANDSVLYVEKEGEPPGPAQAAFKGLVPGRAYNISVQTMSEDEISLPTTAQYRTVPLRPLNVTFDRDFITSNSFRVLWEAPKGISEFDKYQVSVATTRRQSTVPRSNEPVAFFDFRDIAEPGKTFNVIVKTVSGKVTSWPATGDVTLRPLPVRNLRSINDDKTNTMIITWEADPASTQDEYRIVYHELETFNGDTSTLTTDRTRFTLESLLPGRNYSLSVQAVSKKMESNETSIFVVTRPSSPIIEDLKSIRMGLNISWKSDVNSKQEQYEVLYSRNGTSDLRTQKTKESRLVIKNLQPGAGYELKVFAVSHDLRSEPHAYFQAVYPNPPRNMTIETVRSNSVLVHWSPPESGEFTEYSIRYRTDSEQQWVRLPSVRSTEADITDMTKGEKYTIQVNTVSFGVESPVPQEVNTTVPPNPVSNIIQLVDSRNITLEWPKPEGRVESYILKWWPSDNPGRVQTKNVSENKSADDLSTVRVLIGELMPGVQYKFDIQTTSYGILSGITSLYPRTMPLIQSDVVVANGEKEDERDTITLSYTPTPQSSSKFDIYRFSLGDAEIRDKEKLANDTDRKVTFTGLVPGRLYNITVWTVSGGVASLPIQRQDRLYPEPITQLHATNITDTEISLRWDLPKGEYNDFDIAYLTADNLLAQNMTTRNEITISDLRPHRNYTFTVVVRSGTESSVLRSSSPLSASFTTNEAVPGRVERFHPTDVQPSEINFEWSLPSSEANGVIRQFSIAYTNINNLTDAGMQDFESEEAFGVIKNLKPGETYVFKIQAKTAIGFGPEREYRQTMPILAPPRPATQVVPTEVYRSSSTIQIRFRKNYFSDQNGQVRMYTIIVAEDDAKNASGLEMPSWLDVQSYSVWLPYQAIDPYYPFENRSVEDFTIGTENCDNHKIGYCNGPLKSGTTYRVKVRAFTGADKFTDTAYSFPIQTDQDNTSLIVAITVPLTIILVLLVTLLFYKRRRNNCRKTTKDSRANDNMSLPDSVIEQNRPILIKNFAEHYRLMSADSDFRFSEEFEELKHVGRDQPCTFADLPCNRPKNRFTNILPYDHSRFKLQPVDDDEGSDYINANYVPGHNSPREFIVTQGPLHSTRDDFWRMCWESNSRAIVMLTRCFEKGREKCDQYWPNDTVPVFYGDIKVQILNDSHYADWVMTEFMLCRGSEQRILRHFHFTTWPDFGVPNPPQTLVRFVRAFRDRIGAEQRPIVVHCSAGVGRSGTFITLDRILQQINTSDYVDIFGIVYAMRKERVWMVQTEQQYICIHQCLLAVLEGKENIVGPAREMHDNEGYEGQQVQLDENGDVVATIEGHLSHHDLQQAEAEAIDDENAAILHDDQQPLTSSFTGHHTHMPPTTSMSSFGGGGGGHTNVDAPDR*HSVVNQSDNNNSVVIVLVDNKPSSMICKDSKGGNIDVLESQQQQQQQQQQQPNQGGHNITTISAINGYNTLQHRRKSQLITFSSSSCDIKNSLSHEYINGSNGSAANGPPSSGSGSGSGPGSNRASRANVRLSFAEEDVMILPQNHSQQSNHQDDEVFTRRRSLLEVEIGVEVGEDGELAPHEMEEDLEEEDEDEELYMHDEFETHIDTKSNNANDDSGGGSYEDSHALHSSLGGSNRNSLEKDDDDIEVDVISTDVSCYDQLLGSSCNTRNGDDDDIATLVGDGDYSTTKLSKASRLSGAGVGGLVVSGGGGGTAIGGGIAVNGGGVLGNGVGSEAGGGIIYANPFMDDEGIAESGM

Target ORF information:

RefSeq Version	NM_206691.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant G (Ptp10D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206691.3

ORF Insert Sequence:

ATGTTGTACC AGCTAAGTAA AGCCACTACT AGAATCCGGC TCAAAAGGCA AAAAGCCGTT 
CCACAACATC GCTGGCTCTG GAGTTTAGCA TTTCTGGCAG CATTTACACT AAAGGACGTT 
CGATGTGCAG ATCTAGCCAT AAGTATACCC AATAATCCCG GCCTGGACGA TGGGGCCTCC 
TATCGACTGG ACTATAGTCC GCCCTTCGGT TATCCGGAGC CCAACACGAC GATTGCCTCG 
CGGGAAATCG GCGATGAGAT TCAATTCTCA CGCGCCCTAC CGGGCACCAA ATACAACTTT 
TGGCTGTACT ACACGAACTT TACGCACCAC GATTGGCTCA CCTGGACGGT GACGATAACA 
ACGGCTCCCG ATCCGCCGTC GAATCTGTCC GTTCAGGTGA GAAGCGGCAA GAATGCCATC 
ATCCTATGGT CACCGCCCAC CCAGGGCAGC TATACGGCGT TTAAGATCAA GGTGCTCGGA 
CTGTCGGAGG CGTCGAGCAG CTACAACCGC ACCTTTCAGG TGAACGACAA TACATTCCAG 
CACAGCGTCA AGGAGCTGAC ACCCGGAGCC ACCTACCAGG TGCAGGCATA TACCATCTAC 
GATGGCAAGG AGTCGGTGGC CTACACTAGT CGCAACTTCA CCACAAAGCC CAACACTCCG 
GGGAAATTCA TCGTCTGGTT CCGTAATGAG ACGACACTGC TGGTCCTGTG GCAGCCGCCA 
TATCCGGCGG GCATCTACAC GCACTACAAG GTATCCATCG AGCCGCCGGA TGCCAACGAT 
AGTGTGCTCT ATGTGGAAAA GGAGGGCGAA CCGCCCGGAC CGGCGCAGGC TGCCTTCAAG 
GGTCTGGTGC CCGGAAGGGC GTACAACATA TCCGTTCAGA CGATGTCCGA GGATGAGATC 
TCATTGCCGA CGACGGCGCA ATATCGAACG GTGCCGTTGC GTCCGCTGAA CGTGACCTTT 
GACCGTGACT TTATTACCTC CAATTCGTTC CGTGTCCTGT GGGAGGCGCC CAAGGGGATA 
TCCGAATTTG ACAAATACCA GGTATCGGTG GCCACAACAC GACGCCAATC CACAGTGCCG 
CGCAGCAATG AACCGGTGGC ATTCTTTGAT TTTCGCGACA TCGCCGAACC GGGCAAGACG 
TTCAATGTGA TCGTGAAGAC CGTATCCGGC AAGGTTACCT CGTGGCCAGC CACCGGGGAT 
GTGACACTGC GACCACTGCC CGTTCGCAAT TTGCGGAGCA TCAACGATGA CAAGACGAAT 
ACTATGATCA TAACGTGGGA AGCGGATCCG GCCAGCACGC AGGATGAGTA TCGCATTGTA 
TACCACGAAC TGGAGACATT TAATGGTGAC ACCAGTACCC TGACCACGGA TCGGACTCGA 
TTCACACTGG AGAGCCTGCT ACCCGGTCGC AACTACTCAT TGTCCGTCCA GGCGGTATCC 
AAGAAGATGG AATCGAATGA GACTAGCATC TTTGTGGTCA CCCGACCCTC GTCGCCCATC 
ATCGAGGACT TGAAGAGCAT ACGGATGGGT CTGAACATCA GTTGGAAGAG CGATGTCAAC 
TCCAAGCAGG AGCAGTACGA GGTGTTGTAC TCGCGCAACG GAACCAGCGA TTTGCGAACC 
CAAAAGACCA AAGAGTCGCG TCTGGTGATC AAGAATCTGC AGCCAGGTGC TGGCTATGAA 
CTCAAGGTGT TTGCAGTTAG TCACGATTTG CGCAGCGAAC CACATGCCTA TTTCCAAGCA 
GTTTATCCCA ATCCACCACG CAACATGACC ATCGAAACGG TGAGGAGTAA CTCGGTGCTG 
GTACACTGGT CACCGCCGGA AAGCGGTGAA TTTACCGAGT ACTCGATACG CTATCGCACG 
GACAGCGAAC AGCAGTGGGT GCGATTGCCC AGCGTTCGGT CCACGGAGGC GGATATCACC 
GATATGACCA AGGGCGAAAA GTACACCATC CAGGTGAACA CGGTCAGCTT TGGCGTAGAG 
AGTCCAGTGC CCCAGGAGGT GAACACGACG GTGCCGCCGA ATCCGGTGTC CAATATCATT 
CAACTGGTGG ACTCACGGAA CATTACGTTG GAGTGGCCCA AGCCAGAGGG TCGCGTGGAA 
TCGTACATTC TTAAGTGGTG GCCCAGCGAT AATCCCGGTC GTGTCCAGAC CAAGAATGTC 
TCCGAGAACA AGTCGGCCGA CGATTTGTCA ACAGTGCGTG TCCTCATCGG CGAACTGATG 
CCCGGCGTGC AGTACAAGTT TGACATCCAG ACGACGTCGT ATGGAATCCT GTCGGGCATC 
ACCAGTCTGT ATCCGCGCAC CATGCCGCTC ATCCAGTCGG ACGTGGTGGT GGCCAATGGC 
GAGAAGGAGG ACGAGAGGGA CACCATCACT CTGAGCTACA CACCCACACC GCAGTCGTCG 
TCCAAGTTCG ATATCTATCG ATTCTCTCTC GGCGATGCCG AGATCCGGGA CAAGGAGAAG 
CTGGCCAACG ATACGGATCG CAAGGTGACG TTTACGGGTC TGGTGCCTGG TCGATTGTAC 
AACATCACAG TTTGGACTGT GAGCGGTGGT GTGGCCAGTT TGCCCATACA ACGACAGGAT 
CGCCTGTATC CGGAACCCAT CACACAGCTG CATGCCACCA ACATCACGGA TACGGAGATC 
TCATTGCGCT GGGATTTGCC CAAGGGCGAG TACAATGACT TCGATATTGC CTATCTCACG 
GCGGACAATC TATTGGCCCA GAATATGACC ACCAGGAATG AGATCACCAT CAGTGACCTG 
CGACCCCACA GGAACTACAC CTTCACCGTG GTGGTACGTT CCGGCACTGA ATCGTCCGTG 
CTGAGGAGCA GTTCACCATT ATCCGCTAGC TTTACGACCA ATGAAGCGGT GCCCGGGCGA 
GTTGAACGCT TCCATCCCAC GGATGTTCAG CCCAGCGAGA TCAATTTCGA GTGGTCGCTG 
CCATCGAGTG AGGCAAATGG CGTTATTCGC CAGTTCTCGA TAGCCTATAC GAATATCAAT 
AATCTCACGG ACGCAGGCAT GCAGGACTTT GAGTCGGAGG AGGCATTCGG TGTGATCAAG 
AATCTAAAGC CCGGCGAGAC CTATGTGTTC AAGATTCAGG CCAAGACTGC CATCGGTTTC 
GGTCCGGAGC GGGAGTACCG TCAAACGATG CCGATATTAG CGCCACCACG TCCTGCCACC 
CAAGTGGTGC CCACCGAGGT CTATCGCAGC TCATCGACCA TCCAGATTCG GTTTAGGAAG 
AACTACTTCT CGGATCAAAA CGGCCAGGTG CGCATGTACA CGATCATCGT GGCCGAGGAT 
GATGCCAAGA ATGCATCCGG CCTGGAGATG CCCAGCTGGC TGGATGTGCA GTCGTACAGC 
GTTTGGTTGC CCTATCAGGC CATAGATCCG TACTATCCAT TCGAGAATCG ATCCGTAGAG 
GACTTCACCA TCGGTACGGA GAACTGTGAC AACCACAAGA TCGGCTACTG CAACGGACCA 
CTGAAATCGG GAACCACCTA TCGGGTTAAG GTGCGGGCGT TCACCGGAGC GGATAAGTTC 
ACGGATACCG CCTACAGTTT TCCCATTCAG ACAGATCAAG ACAACACCTC ACTGATTGTG 
GCCATTACGG TGCCGTTAAC TATCATCTTG GTGCTCCTGG TGACACTTTT GTTCTACAAA 
CGACGTCGCA ACAATTGCCG TAAGACGACC AAGGATTCGA GGGCCAACGA CAATATGTCC 
CTGCCGGATA GCGTAATCGA GCAGAATCGC CCCATTCTGA TCAAGAACTT TGCCGAGCAC 
TATCGCCTAA TGTCCGCCGA TTCGGACTTC CGTTTCAGCG AGGAATTCGA GGAACTGAAG 
CACGTTGGCC GGGATCAGCC GTGCACTTTT GCCGATCTAC CCTGCAATCG TCCGAAAAAC 
AGGTTCACCA ATATACTGCC CTACGATCAC TCACGTTTCA AGCTTCAGCC GGTGGACGAT 
GATGAGGGTA GTGATTATAT CAATGCCAAT TACGTGCCGG GTCACAATTC ACCGCGCGAG 
TTCATCGTGA CCCAGGGACC ATTGCATTCG ACACGCGATG ACTTCTGGCG AATGTGCTGG 
GAGAGCAACT CGCGGGCCAT AGTCATGCTG ACCAGGTGCT TTGAGAAGGG GCGCGAGAAG 
TGCGACCAGT ATTGGCCAAA TGATACGGTG CCCGTCTTCT ACGGTGACAT CAAGGTGCAG 
ATACTCAACG ACAGTCACTA TGCCGACTGG GTGATGACCG AGTTCATGCT ATGCAGAGGC 
AGCGAACAGC GCATCCTGCG ACACTTCCAC TTCACCACCT GGCCGGACTT CGGTGTTCCC 
AATCCGCCAC AGACACTGGT GCGCTTTGTG CGCGCATTCC GCGATCGAAT TGGTGCGGAA 
CAGCGACCCA TTGTGGTCCA TTGTAGCGCC GGTGTGGGAA GGTCGGGCAC CTTCATCACC 
CTGGATCGCA TCCTGCAACA GATCAACACG TCTGACTATG TGGACATATT TGGCATAGTA 
TATGCCATGC GCAAGGAGCG CGTTTGGATG GTGCAGACGG AGCAGCAGTA TATCTGCATC 
CACCAGTGCC TGCTGGCGGT GCTCGAGGGG AAGGAGAACA TCGTGGGTCC CGCTCGCGAG 
ATGCACGACA ACGAGGGCTA TGAAGGCCAA CAAGTGCAGC TGGACGAGAA CGGTGATGTG 
GTGGCCACCA TTGAGGGTCA CCTGTCGCAT CATGATCTGC AGCAGGCCGA GGCGGAGGCC 
ATTGACGATG AGAATGCGGC GATACTCCAT GATGACCAGC AGCCGCTGAC CAGTAGCTTT 
ACTGGACACC ACACTCACAT GCCGCCCACC ACATCGATGA GCTCATTTGG CGGCGGAGGT 
GGAGGCCATA CAAATGTGGA TGCACCGGAT AGATGACATT CGGTTGTCAA CCAGAGTGAT 
AACAACAATT CGGTGGTTAT CGTCCTGGTT GACAACAAGC CCAGCTCGAT GATCTGCAAG 
GATAGCAAGG GCGGCAACAT CGATGTGCTC GAATCGCAGC AGCAGCAGCA GCAGCAACAG 
CAACAGCAGC CCAACCAGGG AGGCCACAAC ATAACCACCA TATCCGCCAT CAACGGCTAC 
AATACGCTAC AGCATAGACG GAAATCCCAG CTGATAACGT TCAGCTCGTC ATCCTGTGAT 
ATCAAGAACA GTCTTAGTCA TGAGTACATC AATGGATCCA ATGGATCGGC AGCTAATGGT 
CCACCCAGTT CCGGTTCTGG TTCCGGTTCC GGTCCAGGCA GCAATCGGGC CAGTCGCGCC 
AATGTCCGAT TAAGTTTCGC CGAGGAAGAT GTAATGATCT TGCCACAAAA CCACAGTCAG 
CAATCCAATC ATCAAGATGA CGAGGTCTTC ACGCGACGTC GTTCGCTTTT GGAAGTGGAA 
ATCGGTGTGG AGGTAGGCGA AGATGGTGAA CTTGCACCCC ACGAAATGGA AGAGGATCTC 
GAAGAGGAGG ACGAGGATGA GGAGCTGTAT ATGCACGATG AGTTCGAGAC CCACATCGAT 
ACCAAGTCGA ATAATGCCAA CGATGACAGC GGTGGCGGCA GCTACGAGGA CTCCCATGCC 
CTGCACTCCT CGCTGGGGGG CAGTAATCGA AATAGTCTGG AAAAGGATGA CGACGACATC 
GAGGTGGATG TGATCAGCAC AGACGTTAGC TGTTACGATC AACTGCTCGG CAGCAGTTGC 
AACACGCGGA ACGGCGATGA TGACGACATA GCCACGCTGG TCGGCGATGG CGACTATTCC 
ACCACGAAAC TCAGCAAGGC ATCCAGATTA TCCGGCGCTG GAGTCGGCGG ACTAGTGGTC 
AGCGGGGGAG GAGGTGGAAC TGCAATCGGC GGTGGAATCG CTGTTAACGG TGGTGGTGTT 
TTGGGAAATG GTGTTGGTTC CGAGGCTGGC GGCGGCATCA TCTATGCCAA TCCGTTTATG 
GATGACGAGG GCATTGCAGA GTCGGGCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptp10D ( NM_001272525.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259454.1 Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant I (Ptp10D), mRNA.

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CloneID	OFa01303
Clone ID Related Accession (Same CDS sequence)	NM_001272525.1 , NM_001144715.2
Accession Version	NM_001272525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein tyrosine phosphatase 10D, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGTACC AGCTAAGTAA AGCCACTACT AGAATCCGGC TCAAAAGGCA AAAAGCCGTT 
CCACAACATC GCTGGCTCTG GAGTTTAGCA TTTCTGGCAG CATTTACACT AAAGGACGTT 
CGATGTGCAG ATCTAGCCAT AAGTATACCC AATAATCCCG GCCTGGACGA TGGGGCCTCC 
TATCGACTGG ACTATAGTCC GCCCTTCGGT TATCCGGAGC CCAACACGAC GATTGCCTCG 
CGGGAAATCG GCGATGAGAT TCAATTCTCA CGCGCCCTAC CGGGCACCAA ATACAACTTT 
TGGCTGTACT ACACGAACTT TACGCACCAC GATTGGCTCA CCTGGACGGT GACGATAACA 
ACGGCTCCCG ATCCGCCGTC GAATCTGTCC GTTCAGGTGA GAAGCGGCAA GAATGCCATC 
ATCCTATGGT CACCGCCCAC CCAGGGCAGC TATACGGCGT TTAAGATCAA GGTGCTCGGA 
CTGTCGGAGG CGTCGAGCAG CTACAACCGC ACCTTTCAGG TGAACGACAA TACATTCCAG 
CACAGCGTCA AGGAGCTGAC ACCCGGAGCC ACCTACCAGG TGCAGGCATA TACCATCTAC 
GATGGCAAGG AGTCGGTGGC CTACACTAGT CGCAACTTCA CCACAAAGCC CAACACTCCG 
GGGAAATTCA TCGTCTGGTT CCGTAATGAG ACGACACTGC TGGTCCTGTG GCAGCCGCCA 
TATCCGGCGG GCATCTACAC GCACTACAAG GTATCCATCG AGCCGCCGGA TGCCAACGAT 
AGTGTGCTCT ATGTGGAAAA GGAGGGCGAA CCGCCCGGAC CGGCGCAGGC TGCCTTCAAG 
GGTCTGGTGC CCGGAAGGGC GTACAACATA TCCGTTCAGA CGATGTCCGA GGATGAGATC 
TCATTGCCGA CGACGGCGCA ATATCGAACG GTGCCGTTGC GTCCGCTGAA CGTGACCTTT 
GACCGTGACT TTATTACCTC CAATTCGTTC CGTGTCCTGT GGGAGGCGCC CAAGGGGATA 
TCCGAATTTG ACAAATACCA GGTATCGGTG GCCACAACAC GACGCCAATC CACAGTGCCG 
CGCAGCAATG AACCGGTGGC ATTCTTTGAT TTTCGCGACA TCGCCGAACC GGGCAAGACG 
TTCAATGTGA TCGTGAAGAC CGTATCCGGC AAGGTTACCT CGTGGCCAGC CACCGGGGAT 
GTGACACTGC GACCACTGCC CGTTCGCAAT TTGCGGAGCA TCAACGATGA CAAGACGAAT 
ACTATGATCA TAACGTGGGA AGCGGATCCG GCCAGCACGC AGGATGAGTA TCGCATTGTA 
TACCACGAAC TGGAGACATT TAATGGTGAC ACCAGTACCC TGACCACGGA TCGGACTCGA 
TTCACACTGG AGAGCCTGCT ACCCGGTCGC AACTACTCAT TGTCCGTCCA GGCGGTATCC 
AAGAAGATGG AATCGAATGA GACTAGCATC TTTGTGGTCA CCCGACCCTC GTCGCCCATC 
ATCGAGGACT TGAAGAGCAT ACGGATGGGT CTGAACATCA GTTGGAAGAG CGATGTCAAC 
TCCAAGCAGG AGCAGTACGA GGTGTTGTAC TCGCGCAACG GAACCAGCGA TTTGCGAACC 
CAAAAGACCA AAGAGTCGCG TCTGGTGATC AAGAATCTGC AGCCAGGTGC TGGCTATGAA 
CTCAAGGTGT TTGCAGTTAG TCACGATTTG CGCAGCGAAC CACATGCCTA TTTCCAAGCA 
GTTTATCCCA ATCCACCACG CAACATGACC ATCGAAACGG TGAGGAGTAA CTCGGTGCTG 
GTACACTGGT CACCGCCGGA AAGCGGTGAA TTTACCGAGT ACTCGATACG CTATCGCACG 
GACAGCGAAC AGCAGTGGGT GCGATTGCCC AGCGTTCGGT CCACGGAGGC GGATATCACC 
GATATGACCA AGGGCGAAAA GTACACCATC CAGGTGAACA CGGTCAGCTT TGGCGTAGAG 
AGTCCAGTGC CCCAGGAGGT GAACACGACG GTGCCGCCGA ATCCGGTGTC CAATATCATT 
CAACTGGTGG ACTCACGGAA CATTACGTTG GAGTGGCCCA AGCCAGAGGG TCGCGTGGAA 
TCGTACATTC TTAAGTGGTG GCCCAGCGAT AATCCCGGTC GTGTCCAGAC CAAGAATGTC 
TCCGAGAACA AGTCGGCCGA CGATTTGTCA ACAGTGCGTG TCCTCATCGG CGAACTGATG 
CCCGGCGTGC AGTACAAGTT TGACATCCAG ACGACGTCGT ATGGAATCCT GTCGGGCATC 
ACCAGTCTGT ATCCGCGCAC CATGCCGCTC ATCCAGTCGG ACGTGGTGGT GGCCAATGGC 
GAGAAGGAGG ACGAGAGGGA CACCATCACT CTGAGCTACA CACCCACACC GCAGTCGTCG 
TCCAAGTTCG ATATCTATCG ATTCTCTCTC GGCGATGCCG AGATCCGGGA CAAGGAGAAG 
CTGGCCAACG ATACGGATCG CAAGGTGACG TTTACGGGTC TGGTGCCTGG TCGATTGTAC 
AACATCACAG TTTGGACTGT GAGCGGTGGT GTGGCCAGTT TGCCCATACA ACGACAGGAT 
CGCCTGTATC CGGAACCCAT CACACAGCTG CATGCCACCA ACATCACGGA TACGGAGATC 
TCATTGCGCT GGGATTTGCC CAAGGGCGAG TACAATGACT TCGATATTGC CTATCTCACG 
GCGGACAATC TATTGGCCCA GAATATGACC ACCAGGAATG AGATCACCAT CAGTGACCTG 
CGACCCCACA GGAACTACAC CTTCACCGTG GTGGTACGTT CCGGCACTGA ATCGTCCGTG 
CTGAGGAGCA GTTCACCATT ATCCGCTAGC TTTACGACCA ATGAAGCGGT GCCCGGGCGA 
GTTGAACGCT TCCATCCCAC GGATGTTCAG CCCAGCGAGA TCAATTTCGA GTGGTCGCTG 
CCATCGAGTG AGGCAAATGG CGTTATTCGC CAGTTCTCGA TAGCCTATAC GAATATCAAT 
AATCTCACGG ACGCAGGCAT GCAGGACTTT GAGTCGGAGG AGGCATTCGG TGTGATCAAG 
AATCTAAAGC CCGGCGAGAC CTATGTGTTC AAGATTCAGG CCAAGACTGC CATCGGTTTC 
GGTCCGGAGC GGGAGTACCG TCAAACGATG CCGATATTAG CGCCACCACG TCCTGCCACC 
CAAGTGGTGC CCACCGAGGT CTATCGCAGC TCATCGACCA TCCAGATTCG GTTTAGGAAG 
AACTACTTCT CGGATCAAAA CGGCCAGGTG CGCATGTACA CGATCATCGT GGCCGAGGAT 
GATGCCAAGA ATGCATCCGG CCTGGAGATG CCCAGCTGGC TGGATGTGCA GTCGTACAGC 
GTTTGGTTGC CCTATCAGGC CATAGATCCG TACTATCCAT TCGAGAATCG ATCCGTAGAG 
GACTTCACCA TCGGTACGGA GAACTGTGAC AACCACAAGA TCGGCTACTG CAACGGACCA 
CTGAAATCGG GAACCACCTA TCGGGTTAAG GTGCGGGCGT TCACCGGAGC GGATAAGTTC 
ACGGATACCG CCTACAGTTT TCCCATTCAG ACAGATCAAG ACAACACCTC ACTGATTGTG 
GCCATTACGG TGCCGTTAAC TATCATCTTG GTGCTCCTGG TGACACTTTT GTTCTACAAA 
CGACGTCGCA ACAATTGCCG TAAGACGACC AAGGATTCGA GGGCCAACGA CAATATGTCC 
CTGCCGGATA GCGTAATCGA GCAGAATCGC CCCATTCTGA TCAAGAACTT TGCCGAGCAC 
TATCGCCTAA TGTCCGCCGA TTCGGACTTC CGTTTCAGCG AGGAATTCGA GGAACTGAAG 
CACGTTGGCC GGGATCAGCC GTGCACTTTT GCCGATCTAC CCTGCAATCG TCCGAAAAAC 
AGGTTCACCA ATATACTGCC CTACGATCAC TCACGTTTCA AGCTTCAGCC GGTGGACGAT 
GATGAGGGTA GTGATTATAT CAATGCCAAT TACGTGCCGG GTCACAATTC ACCGCGCGAG 
TTCATCGTGA CCCAGGGACC ATTGCATTCG ACACGCGATG ACTTCTGGCG AATGTGCTGG 
GAGAGCAACT CGCGGGCCAT AGTCATGCTG ACCAGGTGCT TTGAGAAGGG GCGCGAGAAG 
TGCGACCAGT ATTGGCCAAA TGATACGGTG CCCGTCTTCT ACGGTGACAT CAAGGTGCAG 
ATACTCAACG ACAGTCACTA TGCCGACTGG GTGATGACCG AGTTCATGCT ATGCAGAGGC 
AGCGAACAGC GCATCCTGCG ACACTTCCAC TTCACCACCT GGCCGGACTT CGGTGTTCCC 
AATCCGCCAC AGACACTGGT GCGCTTTGTG CGCGCATTCC GCGATCGAAT TGGTGCGGAA 
CAGCGACCCA TTGTGGTCCA TTGTAGCGCC GGTGTGGGAA GGTCGGGCAC CTTCATCACC 
CTGGATCGCA TCCTGCAACA GATCAACACG TCTGACTATG TGGACATATT TGGCATAGTA 
TATGCCATGC GCAAGGAGCG CGTTTGGATG GTGCAGACGG AGCAGCAGTA TATCTGCATC 
CACCAGTGCC TGCTGGCGGT GCTCGAGGGG AAGGAGAACA TCGTGGGTCC CGCTCGCGAG 
ATGCACGACA ACGAGGGCTA TGAAGATGAC GAGGGCATTG CAGAGTCGGG CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259454.1
CDS	490..5166
Translation	MLYQLSKATTRIRLKRQKAVPQHRWLWSLAFLAAFTLKDVRCADLAISIPNNPGLDDGASYRLDYSPPFGYPEPNTTIASREIGDEIQFSRALPGTKYNFWLYYTNFTHHDWLTWTVTITTAPDPPSNLSVQVRSGKNAIILWSPPTQGSYTAFKIKVLGLSEASSSYNRTFQVNDNTFQHSVKELTPGATYQVQAYTIYDGKESVAYTSRNFTTKPNTPGKFIVWFRNETTLLVLWQPPYPAGIYTHYKVSIEPPDANDSVLYVEKEGEPPGPAQAAFKGLVPGRAYNISVQTMSEDEISLPTTAQYRTVPLRPLNVTFDRDFITSNSFRVLWEAPKGISEFDKYQVSVATTRRQSTVPRSNEPVAFFDFRDIAEPGKTFNVIVKTVSGKVTSWPATGDVTLRPLPVRNLRSINDDKTNTMIITWEADPASTQDEYRIVYHELETFNGDTSTLTTDRTRFTLESLLPGRNYSLSVQAVSKKMESNETSIFVVTRPSSPIIEDLKSIRMGLNISWKSDVNSKQEQYEVLYSRNGTSDLRTQKTKESRLVIKNLQPGAGYELKVFAVSHDLRSEPHAYFQAVYPNPPRNMTIETVRSNSVLVHWSPPESGEFTEYSIRYRTDSEQQWVRLPSVRSTEADITDMTKGEKYTIQVNTVSFGVESPVPQEVNTTVPPNPVSNIIQLVDSRNITLEWPKPEGRVESYILKWWPSDNPGRVQTKNVSENKSADDLSTVRVLIGELMPGVQYKFDIQTTSYGILSGITSLYPRTMPLIQSDVVVANGEKEDERDTITLSYTPTPQSSSKFDIYRFSLGDAEIRDKEKLANDTDRKVTFTGLVPGRLYNITVWTVSGGVASLPIQRQDRLYPEPITQLHATNITDTEISLRWDLPKGEYNDFDIAYLTADNLLAQNMTTRNEITISDLRPHRNYTFTVVVRSGTESSVLRSSSPLSASFTTNEAVPGRVERFHPTDVQPSEINFEWSLPSSEANGVIRQFSIAYTNINNLTDAGMQDFESEEAFGVIKNLKPGETYVFKIQAKTAIGFGPEREYRQTMPILAPPRPATQVVPTEVYRSSSTIQIRFRKNYFSDQNGQVRMYTIIVAEDDAKNASGLEMPSWLDVQSYSVWLPYQAIDPYYPFENRSVEDFTIGTENCDNHKIGYCNGPLKSGTTYRVKVRAFTGADKFTDTAYSFPIQTDQDNTSLIVAITVPLTIILVLLVTLLFYKRRRNNCRKTTKDSRANDNMSLPDSVIEQNRPILIKNFAEHYRLMSADSDFRFSEEFEELKHVGRDQPCTFADLPCNRPKNRFTNILPYDHSRFKLQPVDDDEGSDYINANYVPGHNSPREFIVTQGPLHSTRDDFWRMCWESNSRAIVMLTRCFEKGREKCDQYWPNDTVPVFYGDIKVQILNDSHYADWVMTEFMLCRGSEQRILRHFHFTTWPDFGVPNPPQTLVRFVRAFRDRIGAEQRPIVVHCSAGVGRSGTFITLDRILQQINTSDYVDIFGIVYAMRKERVWMVQTEQQYICIHQCLLAVLEGKENIVGPAREMHDNEGYEDDEGIAESGM

Target ORF information:

RefSeq Version	NM_001272525.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant I (Ptp10D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272525.1

ORF Insert Sequence:

ATGTTGTACC AGCTAAGTAA AGCCACTACT AGAATCCGGC TCAAAAGGCA AAAAGCCGTT 
CCACAACATC GCTGGCTCTG GAGTTTAGCA TTTCTGGCAG CATTTACACT AAAGGACGTT 
CGATGTGCAG ATCTAGCCAT AAGTATACCC AATAATCCCG GCCTGGACGA TGGGGCCTCC 
TATCGACTGG ACTATAGTCC GCCCTTCGGT TATCCGGAGC CCAACACGAC GATTGCCTCG 
CGGGAAATCG GCGATGAGAT TCAATTCTCA CGCGCCCTAC CGGGCACCAA ATACAACTTT 
TGGCTGTACT ACACGAACTT TACGCACCAC GATTGGCTCA CCTGGACGGT GACGATAACA 
ACGGCTCCCG ATCCGCCGTC GAATCTGTCC GTTCAGGTGA GAAGCGGCAA GAATGCCATC 
ATCCTATGGT CACCGCCCAC CCAGGGCAGC TATACGGCGT TTAAGATCAA GGTGCTCGGA 
CTGTCGGAGG CGTCGAGCAG CTACAACCGC ACCTTTCAGG TGAACGACAA TACATTCCAG 
CACAGCGTCA AGGAGCTGAC ACCCGGAGCC ACCTACCAGG TGCAGGCATA TACCATCTAC 
GATGGCAAGG AGTCGGTGGC CTACACTAGT CGCAACTTCA CCACAAAGCC CAACACTCCG 
GGGAAATTCA TCGTCTGGTT CCGTAATGAG ACGACACTGC TGGTCCTGTG GCAGCCGCCA 
TATCCGGCGG GCATCTACAC GCACTACAAG GTATCCATCG AGCCGCCGGA TGCCAACGAT 
AGTGTGCTCT ATGTGGAAAA GGAGGGCGAA CCGCCCGGAC CGGCGCAGGC TGCCTTCAAG 
GGTCTGGTGC CCGGAAGGGC GTACAACATA TCCGTTCAGA CGATGTCCGA GGATGAGATC 
TCATTGCCGA CGACGGCGCA ATATCGAACG GTGCCGTTGC GTCCGCTGAA CGTGACCTTT 
GACCGTGACT TTATTACCTC CAATTCGTTC CGTGTCCTGT GGGAGGCGCC CAAGGGGATA 
TCCGAATTTG ACAAATACCA GGTATCGGTG GCCACAACAC GACGCCAATC CACAGTGCCG 
CGCAGCAATG AACCGGTGGC ATTCTTTGAT TTTCGCGACA TCGCCGAACC GGGCAAGACG 
TTCAATGTGA TCGTGAAGAC CGTATCCGGC AAGGTTACCT CGTGGCCAGC CACCGGGGAT 
GTGACACTGC GACCACTGCC CGTTCGCAAT TTGCGGAGCA TCAACGATGA CAAGACGAAT 
ACTATGATCA TAACGTGGGA AGCGGATCCG GCCAGCACGC AGGATGAGTA TCGCATTGTA 
TACCACGAAC TGGAGACATT TAATGGTGAC ACCAGTACCC TGACCACGGA TCGGACTCGA 
TTCACACTGG AGAGCCTGCT ACCCGGTCGC AACTACTCAT TGTCCGTCCA GGCGGTATCC 
AAGAAGATGG AATCGAATGA GACTAGCATC TTTGTGGTCA CCCGACCCTC GTCGCCCATC 
ATCGAGGACT TGAAGAGCAT ACGGATGGGT CTGAACATCA GTTGGAAGAG CGATGTCAAC 
TCCAAGCAGG AGCAGTACGA GGTGTTGTAC TCGCGCAACG GAACCAGCGA TTTGCGAACC 
CAAAAGACCA AAGAGTCGCG TCTGGTGATC AAGAATCTGC AGCCAGGTGC TGGCTATGAA 
CTCAAGGTGT TTGCAGTTAG TCACGATTTG CGCAGCGAAC CACATGCCTA TTTCCAAGCA 
GTTTATCCCA ATCCACCACG CAACATGACC ATCGAAACGG TGAGGAGTAA CTCGGTGCTG 
GTACACTGGT CACCGCCGGA AAGCGGTGAA TTTACCGAGT ACTCGATACG CTATCGCACG 
GACAGCGAAC AGCAGTGGGT GCGATTGCCC AGCGTTCGGT CCACGGAGGC GGATATCACC 
GATATGACCA AGGGCGAAAA GTACACCATC CAGGTGAACA CGGTCAGCTT TGGCGTAGAG 
AGTCCAGTGC CCCAGGAGGT GAACACGACG GTGCCGCCGA ATCCGGTGTC CAATATCATT 
CAACTGGTGG ACTCACGGAA CATTACGTTG GAGTGGCCCA AGCCAGAGGG TCGCGTGGAA 
TCGTACATTC TTAAGTGGTG GCCCAGCGAT AATCCCGGTC GTGTCCAGAC CAAGAATGTC 
TCCGAGAACA AGTCGGCCGA CGATTTGTCA ACAGTGCGTG TCCTCATCGG CGAACTGATG 
CCCGGCGTGC AGTACAAGTT TGACATCCAG ACGACGTCGT ATGGAATCCT GTCGGGCATC 
ACCAGTCTGT ATCCGCGCAC CATGCCGCTC ATCCAGTCGG ACGTGGTGGT GGCCAATGGC 
GAGAAGGAGG ACGAGAGGGA CACCATCACT CTGAGCTACA CACCCACACC GCAGTCGTCG 
TCCAAGTTCG ATATCTATCG ATTCTCTCTC GGCGATGCCG AGATCCGGGA CAAGGAGAAG 
CTGGCCAACG ATACGGATCG CAAGGTGACG TTTACGGGTC TGGTGCCTGG TCGATTGTAC 
AACATCACAG TTTGGACTGT GAGCGGTGGT GTGGCCAGTT TGCCCATACA ACGACAGGAT 
CGCCTGTATC CGGAACCCAT CACACAGCTG CATGCCACCA ACATCACGGA TACGGAGATC 
TCATTGCGCT GGGATTTGCC CAAGGGCGAG TACAATGACT TCGATATTGC CTATCTCACG 
GCGGACAATC TATTGGCCCA GAATATGACC ACCAGGAATG AGATCACCAT CAGTGACCTG 
CGACCCCACA GGAACTACAC CTTCACCGTG GTGGTACGTT CCGGCACTGA ATCGTCCGTG 
CTGAGGAGCA GTTCACCATT ATCCGCTAGC TTTACGACCA ATGAAGCGGT GCCCGGGCGA 
GTTGAACGCT TCCATCCCAC GGATGTTCAG CCCAGCGAGA TCAATTTCGA GTGGTCGCTG 
CCATCGAGTG AGGCAAATGG CGTTATTCGC CAGTTCTCGA TAGCCTATAC GAATATCAAT 
AATCTCACGG ACGCAGGCAT GCAGGACTTT GAGTCGGAGG AGGCATTCGG TGTGATCAAG 
AATCTAAAGC CCGGCGAGAC CTATGTGTTC AAGATTCAGG CCAAGACTGC CATCGGTTTC 
GGTCCGGAGC GGGAGTACCG TCAAACGATG CCGATATTAG CGCCACCACG TCCTGCCACC 
CAAGTGGTGC CCACCGAGGT CTATCGCAGC TCATCGACCA TCCAGATTCG GTTTAGGAAG 
AACTACTTCT CGGATCAAAA CGGCCAGGTG CGCATGTACA CGATCATCGT GGCCGAGGAT 
GATGCCAAGA ATGCATCCGG CCTGGAGATG CCCAGCTGGC TGGATGTGCA GTCGTACAGC 
GTTTGGTTGC CCTATCAGGC CATAGATCCG TACTATCCAT TCGAGAATCG ATCCGTAGAG 
GACTTCACCA TCGGTACGGA GAACTGTGAC AACCACAAGA TCGGCTACTG CAACGGACCA 
CTGAAATCGG GAACCACCTA TCGGGTTAAG GTGCGGGCGT TCACCGGAGC GGATAAGTTC 
ACGGATACCG CCTACAGTTT TCCCATTCAG ACAGATCAAG ACAACACCTC ACTGATTGTG 
GCCATTACGG TGCCGTTAAC TATCATCTTG GTGCTCCTGG TGACACTTTT GTTCTACAAA 
CGACGTCGCA ACAATTGCCG TAAGACGACC AAGGATTCGA GGGCCAACGA CAATATGTCC 
CTGCCGGATA GCGTAATCGA GCAGAATCGC CCCATTCTGA TCAAGAACTT TGCCGAGCAC 
TATCGCCTAA TGTCCGCCGA TTCGGACTTC CGTTTCAGCG AGGAATTCGA GGAACTGAAG 
CACGTTGGCC GGGATCAGCC GTGCACTTTT GCCGATCTAC CCTGCAATCG TCCGAAAAAC 
AGGTTCACCA ATATACTGCC CTACGATCAC TCACGTTTCA AGCTTCAGCC GGTGGACGAT 
GATGAGGGTA GTGATTATAT CAATGCCAAT TACGTGCCGG GTCACAATTC ACCGCGCGAG 
TTCATCGTGA CCCAGGGACC ATTGCATTCG ACACGCGATG ACTTCTGGCG AATGTGCTGG 
GAGAGCAACT CGCGGGCCAT AGTCATGCTG ACCAGGTGCT TTGAGAAGGG GCGCGAGAAG 
TGCGACCAGT ATTGGCCAAA TGATACGGTG CCCGTCTTCT ACGGTGACAT CAAGGTGCAG 
ATACTCAACG ACAGTCACTA TGCCGACTGG GTGATGACCG AGTTCATGCT ATGCAGAGGC 
AGCGAACAGC GCATCCTGCG ACACTTCCAC TTCACCACCT GGCCGGACTT CGGTGTTCCC 
AATCCGCCAC AGACACTGGT GCGCTTTGTG CGCGCATTCC GCGATCGAAT TGGTGCGGAA 
CAGCGACCCA TTGTGGTCCA TTGTAGCGCC GGTGTGGGAA GGTCGGGCAC CTTCATCACC 
CTGGATCGCA TCCTGCAACA GATCAACACG TCTGACTATG TGGACATATT TGGCATAGTA 
TATGCCATGC GCAAGGAGCG CGTTTGGATG GTGCAGACGG AGCAGCAGTA TATCTGCATC 
CACCAGTGCC TGCTGGCGGT GCTCGAGGGG AAGGAGAACA TCGTGGGTCC CGCTCGCGAG 
ATGCACGACA ACGAGGGCTA TGAAGATGAC GAGGGCATTG CAGAGTCGGG CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptp10D ( NM_001144715.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138187.1 Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant E (Ptp10D), mRNA.

Price & Availability↑

CloneID	OFa01303
Clone ID Related Accession (Same CDS sequence)	NM_001272525.1 , NM_001144715.2
Accession Version	NM_001144715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein tyrosine phosphatase 10D, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001144715.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGTACC AGCTAAGTAA AGCCACTACT AGAATCCGGC TCAAAAGGCA AAAAGCCGTT 
CCACAACATC GCTGGCTCTG GAGTTTAGCA TTTCTGGCAG CATTTACACT AAAGGACGTT 
CGATGTGCAG ATCTAGCCAT AAGTATACCC AATAATCCCG GCCTGGACGA TGGGGCCTCC 
TATCGACTGG ACTATAGTCC GCCCTTCGGT TATCCGGAGC CCAACACGAC GATTGCCTCG 
CGGGAAATCG GCGATGAGAT TCAATTCTCA CGCGCCCTAC CGGGCACCAA ATACAACTTT 
TGGCTGTACT ACACGAACTT TACGCACCAC GATTGGCTCA CCTGGACGGT GACGATAACA 
ACGGCTCCCG ATCCGCCGTC GAATCTGTCC GTTCAGGTGA GAAGCGGCAA GAATGCCATC 
ATCCTATGGT CACCGCCCAC CCAGGGCAGC TATACGGCGT TTAAGATCAA GGTGCTCGGA 
CTGTCGGAGG CGTCGAGCAG CTACAACCGC ACCTTTCAGG TGAACGACAA TACATTCCAG 
CACAGCGTCA AGGAGCTGAC ACCCGGAGCC ACCTACCAGG TGCAGGCATA TACCATCTAC 
GATGGCAAGG AGTCGGTGGC CTACACTAGT CGCAACTTCA CCACAAAGCC CAACACTCCG 
GGGAAATTCA TCGTCTGGTT CCGTAATGAG ACGACACTGC TGGTCCTGTG GCAGCCGCCA 
TATCCGGCGG GCATCTACAC GCACTACAAG GTATCCATCG AGCCGCCGGA TGCCAACGAT 
AGTGTGCTCT ATGTGGAAAA GGAGGGCGAA CCGCCCGGAC CGGCGCAGGC TGCCTTCAAG 
GGTCTGGTGC CCGGAAGGGC GTACAACATA TCCGTTCAGA CGATGTCCGA GGATGAGATC 
TCATTGCCGA CGACGGCGCA ATATCGAACG GTGCCGTTGC GTCCGCTGAA CGTGACCTTT 
GACCGTGACT TTATTACCTC CAATTCGTTC CGTGTCCTGT GGGAGGCGCC CAAGGGGATA 
TCCGAATTTG ACAAATACCA GGTATCGGTG GCCACAACAC GACGCCAATC CACAGTGCCG 
CGCAGCAATG AACCGGTGGC ATTCTTTGAT TTTCGCGACA TCGCCGAACC GGGCAAGACG 
TTCAATGTGA TCGTGAAGAC CGTATCCGGC AAGGTTACCT CGTGGCCAGC CACCGGGGAT 
GTGACACTGC GACCACTGCC CGTTCGCAAT TTGCGGAGCA TCAACGATGA CAAGACGAAT 
ACTATGATCA TAACGTGGGA AGCGGATCCG GCCAGCACGC AGGATGAGTA TCGCATTGTA 
TACCACGAAC TGGAGACATT TAATGGTGAC ACCAGTACCC TGACCACGGA TCGGACTCGA 
TTCACACTGG AGAGCCTGCT ACCCGGTCGC AACTACTCAT TGTCCGTCCA GGCGGTATCC 
AAGAAGATGG AATCGAATGA GACTAGCATC TTTGTGGTCA CCCGACCCTC GTCGCCCATC 
ATCGAGGACT TGAAGAGCAT ACGGATGGGT CTGAACATCA GTTGGAAGAG CGATGTCAAC 
TCCAAGCAGG AGCAGTACGA GGTGTTGTAC TCGCGCAACG GAACCAGCGA TTTGCGAACC 
CAAAAGACCA AAGAGTCGCG TCTGGTGATC AAGAATCTGC AGCCAGGTGC TGGCTATGAA 
CTCAAGGTGT TTGCAGTTAG TCACGATTTG CGCAGCGAAC CACATGCCTA TTTCCAAGCA 
GTTTATCCCA ATCCACCACG CAACATGACC ATCGAAACGG TGAGGAGTAA CTCGGTGCTG 
GTACACTGGT CACCGCCGGA AAGCGGTGAA TTTACCGAGT ACTCGATACG CTATCGCACG 
GACAGCGAAC AGCAGTGGGT GCGATTGCCC AGCGTTCGGT CCACGGAGGC GGATATCACC 
GATATGACCA AGGGCGAAAA GTACACCATC CAGGTGAACA CGGTCAGCTT TGGCGTAGAG 
AGTCCAGTGC CCCAGGAGGT GAACACGACG GTGCCGCCGA ATCCGGTGTC CAATATCATT 
CAACTGGTGG ACTCACGGAA CATTACGTTG GAGTGGCCCA AGCCAGAGGG TCGCGTGGAA 
TCGTACATTC TTAAGTGGTG GCCCAGCGAT AATCCCGGTC GTGTCCAGAC CAAGAATGTC 
TCCGAGAACA AGTCGGCCGA CGATTTGTCA ACAGTGCGTG TCCTCATCGG CGAACTGATG 
CCCGGCGTGC AGTACAAGTT TGACATCCAG ACGACGTCGT ATGGAATCCT GTCGGGCATC 
ACCAGTCTGT ATCCGCGCAC CATGCCGCTC ATCCAGTCGG ACGTGGTGGT GGCCAATGGC 
GAGAAGGAGG ACGAGAGGGA CACCATCACT CTGAGCTACA CACCCACACC GCAGTCGTCG 
TCCAAGTTCG ATATCTATCG ATTCTCTCTC GGCGATGCCG AGATCCGGGA CAAGGAGAAG 
CTGGCCAACG ATACGGATCG CAAGGTGACG TTTACGGGTC TGGTGCCTGG TCGATTGTAC 
AACATCACAG TTTGGACTGT GAGCGGTGGT GTGGCCAGTT TGCCCATACA ACGACAGGAT 
CGCCTGTATC CGGAACCCAT CACACAGCTG CATGCCACCA ACATCACGGA TACGGAGATC 
TCATTGCGCT GGGATTTGCC CAAGGGCGAG TACAATGACT TCGATATTGC CTATCTCACG 
GCGGACAATC TATTGGCCCA GAATATGACC ACCAGGAATG AGATCACCAT CAGTGACCTG 
CGACCCCACA GGAACTACAC CTTCACCGTG GTGGTACGTT CCGGCACTGA ATCGTCCGTG 
CTGAGGAGCA GTTCACCATT ATCCGCTAGC TTTACGACCA ATGAAGCGGT GCCCGGGCGA 
GTTGAACGCT TCCATCCCAC GGATGTTCAG CCCAGCGAGA TCAATTTCGA GTGGTCGCTG 
CCATCGAGTG AGGCAAATGG CGTTATTCGC CAGTTCTCGA TAGCCTATAC GAATATCAAT 
AATCTCACGG ACGCAGGCAT GCAGGACTTT GAGTCGGAGG AGGCATTCGG TGTGATCAAG 
AATCTAAAGC CCGGCGAGAC CTATGTGTTC AAGATTCAGG CCAAGACTGC CATCGGTTTC 
GGTCCGGAGC GGGAGTACCG TCAAACGATG CCGATATTAG CGCCACCACG TCCTGCCACC 
CAAGTGGTGC CCACCGAGGT CTATCGCAGC TCATCGACCA TCCAGATTCG GTTTAGGAAG 
AACTACTTCT CGGATCAAAA CGGCCAGGTG CGCATGTACA CGATCATCGT GGCCGAGGAT 
GATGCCAAGA ATGCATCCGG CCTGGAGATG CCCAGCTGGC TGGATGTGCA GTCGTACAGC 
GTTTGGTTGC CCTATCAGGC CATAGATCCG TACTATCCAT TCGAGAATCG ATCCGTAGAG 
GACTTCACCA TCGGTACGGA GAACTGTGAC AACCACAAGA TCGGCTACTG CAACGGACCA 
CTGAAATCGG GAACCACCTA TCGGGTTAAG GTGCGGGCGT TCACCGGAGC GGATAAGTTC 
ACGGATACCG CCTACAGTTT TCCCATTCAG ACAGATCAAG ACAACACCTC ACTGATTGTG 
GCCATTACGG TGCCGTTAAC TATCATCTTG GTGCTCCTGG TGACACTTTT GTTCTACAAA 
CGACGTCGCA ACAATTGCCG TAAGACGACC AAGGATTCGA GGGCCAACGA CAATATGTCC 
CTGCCGGATA GCGTAATCGA GCAGAATCGC CCCATTCTGA TCAAGAACTT TGCCGAGCAC 
TATCGCCTAA TGTCCGCCGA TTCGGACTTC CGTTTCAGCG AGGAATTCGA GGAACTGAAG 
CACGTTGGCC GGGATCAGCC GTGCACTTTT GCCGATCTAC CCTGCAATCG TCCGAAAAAC 
AGGTTCACCA ATATACTGCC CTACGATCAC TCACGTTTCA AGCTTCAGCC GGTGGACGAT 
GATGAGGGTA GTGATTATAT CAATGCCAAT TACGTGCCGG GTCACAATTC ACCGCGCGAG 
TTCATCGTGA CCCAGGGACC ATTGCATTCG ACACGCGATG ACTTCTGGCG AATGTGCTGG 
GAGAGCAACT CGCGGGCCAT AGTCATGCTG ACCAGGTGCT TTGAGAAGGG GCGCGAGAAG 
TGCGACCAGT ATTGGCCAAA TGATACGGTG CCCGTCTTCT ACGGTGACAT CAAGGTGCAG 
ATACTCAACG ACAGTCACTA TGCCGACTGG GTGATGACCG AGTTCATGCT ATGCAGAGGC 
AGCGAACAGC GCATCCTGCG ACACTTCCAC TTCACCACCT GGCCGGACTT CGGTGTTCCC 
AATCCGCCAC AGACACTGGT GCGCTTTGTG CGCGCATTCC GCGATCGAAT TGGTGCGGAA 
CAGCGACCCA TTGTGGTCCA TTGTAGCGCC GGTGTGGGAA GGTCGGGCAC CTTCATCACC 
CTGGATCGCA TCCTGCAACA GATCAACACG TCTGACTATG TGGACATATT TGGCATAGTA 
TATGCCATGC GCAAGGAGCG CGTTTGGATG GTGCAGACGG AGCAGCAGTA TATCTGCATC 
CACCAGTGCC TGCTGGCGGT GCTCGAGGGG AAGGAGAACA TCGTGGGTCC CGCTCGCGAG 
ATGCACGACA ACGAGGGCTA TGAAGATGAC GAGGGCATTG CAGAGTCGGG CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138187.1
CDS	617..5293
Translation	MLYQLSKATTRIRLKRQKAVPQHRWLWSLAFLAAFTLKDVRCADLAISIPNNPGLDDGASYRLDYSPPFGYPEPNTTIASREIGDEIQFSRALPGTKYNFWLYYTNFTHHDWLTWTVTITTAPDPPSNLSVQVRSGKNAIILWSPPTQGSYTAFKIKVLGLSEASSSYNRTFQVNDNTFQHSVKELTPGATYQVQAYTIYDGKESVAYTSRNFTTKPNTPGKFIVWFRNETTLLVLWQPPYPAGIYTHYKVSIEPPDANDSVLYVEKEGEPPGPAQAAFKGLVPGRAYNISVQTMSEDEISLPTTAQYRTVPLRPLNVTFDRDFITSNSFRVLWEAPKGISEFDKYQVSVATTRRQSTVPRSNEPVAFFDFRDIAEPGKTFNVIVKTVSGKVTSWPATGDVTLRPLPVRNLRSINDDKTNTMIITWEADPASTQDEYRIVYHELETFNGDTSTLTTDRTRFTLESLLPGRNYSLSVQAVSKKMESNETSIFVVTRPSSPIIEDLKSIRMGLNISWKSDVNSKQEQYEVLYSRNGTSDLRTQKTKESRLVIKNLQPGAGYELKVFAVSHDLRSEPHAYFQAVYPNPPRNMTIETVRSNSVLVHWSPPESGEFTEYSIRYRTDSEQQWVRLPSVRSTEADITDMTKGEKYTIQVNTVSFGVESPVPQEVNTTVPPNPVSNIIQLVDSRNITLEWPKPEGRVESYILKWWPSDNPGRVQTKNVSENKSADDLSTVRVLIGELMPGVQYKFDIQTTSYGILSGITSLYPRTMPLIQSDVVVANGEKEDERDTITLSYTPTPQSSSKFDIYRFSLGDAEIRDKEKLANDTDRKVTFTGLVPGRLYNITVWTVSGGVASLPIQRQDRLYPEPITQLHATNITDTEISLRWDLPKGEYNDFDIAYLTADNLLAQNMTTRNEITISDLRPHRNYTFTVVVRSGTESSVLRSSSPLSASFTTNEAVPGRVERFHPTDVQPSEINFEWSLPSSEANGVIRQFSIAYTNINNLTDAGMQDFESEEAFGVIKNLKPGETYVFKIQAKTAIGFGPEREYRQTMPILAPPRPATQVVPTEVYRSSSTIQIRFRKNYFSDQNGQVRMYTIIVAEDDAKNASGLEMPSWLDVQSYSVWLPYQAIDPYYPFENRSVEDFTIGTENCDNHKIGYCNGPLKSGTTYRVKVRAFTGADKFTDTAYSFPIQTDQDNTSLIVAITVPLTIILVLLVTLLFYKRRRNNCRKTTKDSRANDNMSLPDSVIEQNRPILIKNFAEHYRLMSADSDFRFSEEFEELKHVGRDQPCTFADLPCNRPKNRFTNILPYDHSRFKLQPVDDDEGSDYINANYVPGHNSPREFIVTQGPLHSTRDDFWRMCWESNSRAIVMLTRCFEKGREKCDQYWPNDTVPVFYGDIKVQILNDSHYADWVMTEFMLCRGSEQRILRHFHFTTWPDFGVPNPPQTLVRFVRAFRDRIGAEQRPIVVHCSAGVGRSGTFITLDRILQQINTSDYVDIFGIVYAMRKERVWMVQTEQQYICIHQCLLAVLEGKENIVGPAREMHDNEGYEDDEGIAESGM

Target ORF information:

RefSeq Version	NM_001144715.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant E (Ptp10D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144715.2

ORF Insert Sequence:

ATGTTGTACC AGCTAAGTAA AGCCACTACT AGAATCCGGC TCAAAAGGCA AAAAGCCGTT 
CCACAACATC GCTGGCTCTG GAGTTTAGCA TTTCTGGCAG CATTTACACT AAAGGACGTT 
CGATGTGCAG ATCTAGCCAT AAGTATACCC AATAATCCCG GCCTGGACGA TGGGGCCTCC 
TATCGACTGG ACTATAGTCC GCCCTTCGGT TATCCGGAGC CCAACACGAC GATTGCCTCG 
CGGGAAATCG GCGATGAGAT TCAATTCTCA CGCGCCCTAC CGGGCACCAA ATACAACTTT 
TGGCTGTACT ACACGAACTT TACGCACCAC GATTGGCTCA CCTGGACGGT GACGATAACA 
ACGGCTCCCG ATCCGCCGTC GAATCTGTCC GTTCAGGTGA GAAGCGGCAA GAATGCCATC 
ATCCTATGGT CACCGCCCAC CCAGGGCAGC TATACGGCGT TTAAGATCAA GGTGCTCGGA 
CTGTCGGAGG CGTCGAGCAG CTACAACCGC ACCTTTCAGG TGAACGACAA TACATTCCAG 
CACAGCGTCA AGGAGCTGAC ACCCGGAGCC ACCTACCAGG TGCAGGCATA TACCATCTAC 
GATGGCAAGG AGTCGGTGGC CTACACTAGT CGCAACTTCA CCACAAAGCC CAACACTCCG 
GGGAAATTCA TCGTCTGGTT CCGTAATGAG ACGACACTGC TGGTCCTGTG GCAGCCGCCA 
TATCCGGCGG GCATCTACAC GCACTACAAG GTATCCATCG AGCCGCCGGA TGCCAACGAT 
AGTGTGCTCT ATGTGGAAAA GGAGGGCGAA CCGCCCGGAC CGGCGCAGGC TGCCTTCAAG 
GGTCTGGTGC CCGGAAGGGC GTACAACATA TCCGTTCAGA CGATGTCCGA GGATGAGATC 
TCATTGCCGA CGACGGCGCA ATATCGAACG GTGCCGTTGC GTCCGCTGAA CGTGACCTTT 
GACCGTGACT TTATTACCTC CAATTCGTTC CGTGTCCTGT GGGAGGCGCC CAAGGGGATA 
TCCGAATTTG ACAAATACCA GGTATCGGTG GCCACAACAC GACGCCAATC CACAGTGCCG 
CGCAGCAATG AACCGGTGGC ATTCTTTGAT TTTCGCGACA TCGCCGAACC GGGCAAGACG 
TTCAATGTGA TCGTGAAGAC CGTATCCGGC AAGGTTACCT CGTGGCCAGC CACCGGGGAT 
GTGACACTGC GACCACTGCC CGTTCGCAAT TTGCGGAGCA TCAACGATGA CAAGACGAAT 
ACTATGATCA TAACGTGGGA AGCGGATCCG GCCAGCACGC AGGATGAGTA TCGCATTGTA 
TACCACGAAC TGGAGACATT TAATGGTGAC ACCAGTACCC TGACCACGGA TCGGACTCGA 
TTCACACTGG AGAGCCTGCT ACCCGGTCGC AACTACTCAT TGTCCGTCCA GGCGGTATCC 
AAGAAGATGG AATCGAATGA GACTAGCATC TTTGTGGTCA CCCGACCCTC GTCGCCCATC 
ATCGAGGACT TGAAGAGCAT ACGGATGGGT CTGAACATCA GTTGGAAGAG CGATGTCAAC 
TCCAAGCAGG AGCAGTACGA GGTGTTGTAC TCGCGCAACG GAACCAGCGA TTTGCGAACC 
CAAAAGACCA AAGAGTCGCG TCTGGTGATC AAGAATCTGC AGCCAGGTGC TGGCTATGAA 
CTCAAGGTGT TTGCAGTTAG TCACGATTTG CGCAGCGAAC CACATGCCTA TTTCCAAGCA 
GTTTATCCCA ATCCACCACG CAACATGACC ATCGAAACGG TGAGGAGTAA CTCGGTGCTG 
GTACACTGGT CACCGCCGGA AAGCGGTGAA TTTACCGAGT ACTCGATACG CTATCGCACG 
GACAGCGAAC AGCAGTGGGT GCGATTGCCC AGCGTTCGGT CCACGGAGGC GGATATCACC 
GATATGACCA AGGGCGAAAA GTACACCATC CAGGTGAACA CGGTCAGCTT TGGCGTAGAG 
AGTCCAGTGC CCCAGGAGGT GAACACGACG GTGCCGCCGA ATCCGGTGTC CAATATCATT 
CAACTGGTGG ACTCACGGAA CATTACGTTG GAGTGGCCCA AGCCAGAGGG TCGCGTGGAA 
TCGTACATTC TTAAGTGGTG GCCCAGCGAT AATCCCGGTC GTGTCCAGAC CAAGAATGTC 
TCCGAGAACA AGTCGGCCGA CGATTTGTCA ACAGTGCGTG TCCTCATCGG CGAACTGATG 
CCCGGCGTGC AGTACAAGTT TGACATCCAG ACGACGTCGT ATGGAATCCT GTCGGGCATC 
ACCAGTCTGT ATCCGCGCAC CATGCCGCTC ATCCAGTCGG ACGTGGTGGT GGCCAATGGC 
GAGAAGGAGG ACGAGAGGGA CACCATCACT CTGAGCTACA CACCCACACC GCAGTCGTCG 
TCCAAGTTCG ATATCTATCG ATTCTCTCTC GGCGATGCCG AGATCCGGGA CAAGGAGAAG 
CTGGCCAACG ATACGGATCG CAAGGTGACG TTTACGGGTC TGGTGCCTGG TCGATTGTAC 
AACATCACAG TTTGGACTGT GAGCGGTGGT GTGGCCAGTT TGCCCATACA ACGACAGGAT 
CGCCTGTATC CGGAACCCAT CACACAGCTG CATGCCACCA ACATCACGGA TACGGAGATC 
TCATTGCGCT GGGATTTGCC CAAGGGCGAG TACAATGACT TCGATATTGC CTATCTCACG 
GCGGACAATC TATTGGCCCA GAATATGACC ACCAGGAATG AGATCACCAT CAGTGACCTG 
CGACCCCACA GGAACTACAC CTTCACCGTG GTGGTACGTT CCGGCACTGA ATCGTCCGTG 
CTGAGGAGCA GTTCACCATT ATCCGCTAGC TTTACGACCA ATGAAGCGGT GCCCGGGCGA 
GTTGAACGCT TCCATCCCAC GGATGTTCAG CCCAGCGAGA TCAATTTCGA GTGGTCGCTG 
CCATCGAGTG AGGCAAATGG CGTTATTCGC CAGTTCTCGA TAGCCTATAC GAATATCAAT 
AATCTCACGG ACGCAGGCAT GCAGGACTTT GAGTCGGAGG AGGCATTCGG TGTGATCAAG 
AATCTAAAGC CCGGCGAGAC CTATGTGTTC AAGATTCAGG CCAAGACTGC CATCGGTTTC 
GGTCCGGAGC GGGAGTACCG TCAAACGATG CCGATATTAG CGCCACCACG TCCTGCCACC 
CAAGTGGTGC CCACCGAGGT CTATCGCAGC TCATCGACCA TCCAGATTCG GTTTAGGAAG 
AACTACTTCT CGGATCAAAA CGGCCAGGTG CGCATGTACA CGATCATCGT GGCCGAGGAT 
GATGCCAAGA ATGCATCCGG CCTGGAGATG CCCAGCTGGC TGGATGTGCA GTCGTACAGC 
GTTTGGTTGC CCTATCAGGC CATAGATCCG TACTATCCAT TCGAGAATCG ATCCGTAGAG 
GACTTCACCA TCGGTACGGA GAACTGTGAC AACCACAAGA TCGGCTACTG CAACGGACCA 
CTGAAATCGG GAACCACCTA TCGGGTTAAG GTGCGGGCGT TCACCGGAGC GGATAAGTTC 
ACGGATACCG CCTACAGTTT TCCCATTCAG ACAGATCAAG ACAACACCTC ACTGATTGTG 
GCCATTACGG TGCCGTTAAC TATCATCTTG GTGCTCCTGG TGACACTTTT GTTCTACAAA 
CGACGTCGCA ACAATTGCCG TAAGACGACC AAGGATTCGA GGGCCAACGA CAATATGTCC 
CTGCCGGATA GCGTAATCGA GCAGAATCGC CCCATTCTGA TCAAGAACTT TGCCGAGCAC 
TATCGCCTAA TGTCCGCCGA TTCGGACTTC CGTTTCAGCG AGGAATTCGA GGAACTGAAG 
CACGTTGGCC GGGATCAGCC GTGCACTTTT GCCGATCTAC CCTGCAATCG TCCGAAAAAC 
AGGTTCACCA ATATACTGCC CTACGATCAC TCACGTTTCA AGCTTCAGCC GGTGGACGAT 
GATGAGGGTA GTGATTATAT CAATGCCAAT TACGTGCCGG GTCACAATTC ACCGCGCGAG 
TTCATCGTGA CCCAGGGACC ATTGCATTCG ACACGCGATG ACTTCTGGCG AATGTGCTGG 
GAGAGCAACT CGCGGGCCAT AGTCATGCTG ACCAGGTGCT TTGAGAAGGG GCGCGAGAAG 
TGCGACCAGT ATTGGCCAAA TGATACGGTG CCCGTCTTCT ACGGTGACAT CAAGGTGCAG 
ATACTCAACG ACAGTCACTA TGCCGACTGG GTGATGACCG AGTTCATGCT ATGCAGAGGC 
AGCGAACAGC GCATCCTGCG ACACTTCCAC TTCACCACCT GGCCGGACTT CGGTGTTCCC 
AATCCGCCAC AGACACTGGT GCGCTTTGTG CGCGCATTCC GCGATCGAAT TGGTGCGGAA 
CAGCGACCCA TTGTGGTCCA TTGTAGCGCC GGTGTGGGAA GGTCGGGCAC CTTCATCACC 
CTGGATCGCA TCCTGCAACA GATCAACACG TCTGACTATG TGGACATATT TGGCATAGTA 
TATGCCATGC GCAAGGAGCG CGTTTGGATG GTGCAGACGG AGCAGCAGTA TATCTGCATC 
CACCAGTGCC TGCTGGCGGT GCTCGAGGGG AAGGAGAACA TCGTGGGTCC CGCTCGCGAG 
ATGCACGACA ACGAGGGCTA TGAAGATGAC GAGGGCATTG CAGAGTCGGG CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptp10D ( NM_167292.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727544.2 Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant B (Ptp10D), mRNA.

Price & Availability↑

CloneID	OFa01304
Clone ID Related Accession (Same CDS sequence)	NM_167292.3
Accession Version	NM_167292.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein tyrosine phosphatase 10D, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167292.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGTACC AGCTAAGTAA AGCCACTACT AGAATCCGGC TCAAAAGGCA AAAAGCCGTT 
CCACAACATC GCTGGCTCTG GAGTTTAGCA TTTCTGGCAG CATTTACACT AAAGGACGTT 
CGATGTGCAG ATCTAGCCAT AAGTATACCC AATAATCCCG GCCTGGACGA TGGGGCCTCC 
TATCGACTGG ACTATAGTCC GCCCTTCGGT TATCCGGAGC CCAACACGAC GATTGCCTCG 
CGGGAAATCG GCGATGAGAT TCAATTCTCA CGCGCCCTAC CGGGCACCAA ATACAACTTT 
TGGCTGTACT ACACGAACTT TACGCACCAC GATTGGCTCA CCTGGACGGT GACGATAACA 
ACGGCTCCCG ATCCGCCGTC GAATCTGTCC GTTCAGGTGA GAAGCGGCAA GAATGCCATC 
ATCCTATGGT CACCGCCCAC CCAGGGCAGC TATACGGCGT TTAAGATCAA GGTGCTCGGA 
CTGTCGGAGG CGTCGAGCAG CTACAACCGC ACCTTTCAGG TGAACGACAA TACATTCCAG 
CACAGCGTCA AGGAGCTGAC ACCCGGAGCC ACCTACCAGG TGCAGGCATA TACCATCTAC 
GATGGCAAGG AGTCGGTGGC CTACACTAGT CGCAACTTCA CCACAAAGCC CAACACTCCG 
GGGAAATTCA TCGTCTGGTT CCGTAATGAG ACGACACTGC TGGTCCTGTG GCAGCCGCCA 
TATCCGGCGG GCATCTACAC GCACTACAAG GTATCCATCG AGCCGCCGGA TGCCAACGAT 
AGTGTGCTCT ATGTGGAAAA GGAGGGCGAA CCGCCCGGAC CGGCGCAGGC TGCCTTCAAG 
GGTCTGGTGC CCGGAAGGGC GTACAACATA TCCGTTCAGA CGATGTCCGA GGATGAGATC 
TCATTGCCGA CGACGGCGCA ATATCGAACG GTGCCGTTGC GTCCGCTGAA CGTGACCTTT 
GACCGTGACT TTATTACCTC CAATTCGTTC CGTGTCCTGT GGGAGGCGCC CAAGGGGATA 
TCCGAATTTG ACAAATACCA GGTATCGGTG GCCACAACAC GACGCCAATC CACAGTGCCG 
CGCAGCAATG AACCGGTGGC ATTCTTTGAT TTTCGCGACA TCGCCGAACC GGGCAAGACG 
TTCAATGTGA TCGTGAAGAC CGTATCCGGC AAGGTTACCT CGTGGCCAGC CACCGGGGAT 
GTGACACTGC GACCACTGCC CGTTCGCAAT TTGCGGAGCA TCAACGATGA CAAGACGAAT 
ACTATGATCA TAACGTGGGA AGCGGATCCG GCCAGCACGC AGGATGAGTA TCGCATTGTA 
TACCACGAAC TGGAGACATT TAATGGTGAC ACCAGTACCC TGACCACGGA TCGGACTCGA 
TTCACACTGG AGAGCCTGCT ACCCGGTCGC AACTACTCAT TGTCCGTCCA GGCGGTATCC 
AAGAAGATGG AATCGAATGA GACTAGCATC TTTGTGGTCA CCCGACCCTC GTCGCCCATC 
ATCGAGGACT TGAAGAGCAT ACGGATGGGT CTGAACATCA GTTGGAAGAG CGATGTCAAC 
TCCAAGCAGG AGCAGTACGA GGTGTTGTAC TCGCGCAACG GAACCAGCGA TTTGCGAACC 
CAAAAGACCA AAGAGTCGCG TCTGGTGATC AAGAATCTGC AGCCAGGTGC TGGCTATGAA 
CTCAAGGTGT TTGCAGTTAG TCACGATTTG CGCAGCGAAC CACATGCCTA TTTCCAAGCA 
GTTTATCCCA ATCCACCACG CAACATGACC ATCGAAACGG TGAGGAGTAA CTCGGTGCTG 
GTACACTGGT CACCGCCGGA AAGCGGTGAA TTTACCGAGT ACTCGATACG CTATCGCACG 
GACAGCGAAC AGCAGTGGGT GCGATTGCCC AGCGTTCGGT CCACGGAGGC GGATATCACC 
GATATGACCA AGGGCGAAAA GTACACCATC CAGGTGAACA CGGTCAGCTT TGGCGTAGAG 
AGTCCAGTGC CCCAGGAGGT GAACACGACG GTGCCGCCGA ATCCGGTGTC CAATATCATT 
CAACTGGTGG ACTCACGGAA CATTACGTTG GAGTGGCCCA AGCCAGAGGG TCGCGTGGAA 
TCGTACATTC TTAAGTGGTG GCCCAGCGAT AATCCCGGTC GTGTCCAGAC CAAGAATGTC 
TCCGAGAACA AGTCGGCCGA CGATTTGTCA ACAGTGCGTG TCCTCATCGG CGAACTGATG 
CCCGGCGTGC AGTACAAGTT TGACATCCAG ACGACGTCGT ATGGAATCCT GTCGGGCATC 
ACCAGTCTGT ATCCGCGCAC CATGCCGCTC ATCCAGTCGG ACGTGGTGGT GGCCAATGGC 
GAGAAGGAGG ACGAGAGGGA CACCATCACT CTGAGCTACA CACCCACACC GCAGTCGTCG 
TCCAAGTTCG ATATCTATCG ATTCTCTCTC GGCGATGCCG AGATCCGGGA CAAGGAGAAG 
CTGGCCAACG ATACGGATCG CAAGGTGACG TTTACGGGTC TGGTGCCTGG TCGATTGTAC 
AACATCACAG TTTGGACTGT GAGCGGTGGT GTGGCCAGTT TGCCCATACA ACGACAGGAT 
CGCCTGTATC CGGAACCCAT CACACAGCTG CATGCCACCA ACATCACGGA TACGGAGATC 
TCATTGCGCT GGGATTTGCC CAAGGGCGAG TACAATGACT TCGATATTGC CTATCTCACG 
GCGGACAATC TATTGGCCCA GAATATGACC ACCAGGAATG AGATCACCAT CAGTGACCTG 
CGACCCCACA GGAACTACAC CTTCACCGTG GTGGTACGTT CCGGCACTGA ATCGTCCGTG 
CTGAGGAGCA GTTCACCATT ATCCGCTAGC TTTACGACCA ATGAAGCGGT GCCCGGGCGA 
GTTGAACGCT TCCATCCCAC GGATGTTCAG CCCAGCGAGA TCAATTTCGA GTGGTCGCTG 
CCATCGAGTG AGGCAAATGG CGTTATTCGC CAGTTCTCGA TAGCCTATAC GAATATCAAT 
AATCTCACGG ACGCAGGCAT GCAGGACTTT GAGTCGGAGG AGGCATTCGG TGTGATCAAG 
AATCTAAAGC CCGGCGAGAC CTATGTGTTC AAGATTCAGG CCAAGACTGC CATCGGTTTC 
GGTCCGGAGC GGGAGTACCG TCAAACGATG CCGATATTAG CGCCACCACG TCCTGCCACC 
CAAGTGGTGC CCACCGAGGT CTATCGCAGC TCATCGACCA TCCAGATTCG GTTTAGGAAG 
AACTACTTCT CGGATCAAAA CGGCCAGGTG CGCATGTACA CGATCATCGT GGCCGAGGAT 
GATGCCAAGA ATGCATCCGG CCTGGAGATG CCCAGCTGGC TGGATGTGCA GTCGTACAGC 
GTTTGGTTGC CCTATCAGGC CATAGATCCG TACTATCCAT TCGAGAATCG ATCCGTAGAG 
GACTTCACCA TCGGTACGGA GAACTGTGAC AACCACAAGA TCGGCTACTG CAACGGACCA 
CTGAAATCGG GAACCACCTA TCGGGTTAAG GTGCGGGCGT TCACCGGAGC GGATAAGTTC 
ACGGATACCG CCTACAGTTT TCCCATTCAG ACAGATCAAG ACAACACCTC ACTGATTGTG 
GCCATTACGG TGCCGTTAAC TATCATCTTG GTGCTCCTGG TGACACTTTT GTTCTACAAA 
CGACGTCGCA ACAATTGCCG TAAGACGACC AAGGATTCGA GGGCCAACGA CAATATGTCC 
CTGCCGGATA GCGTAATCGA GCAGAATCGC CCCATTCTGA TCAAGAACTT TGCCGAGCAC 
TATCGCCTAA TGTCCGCCGA TTCGGACTTC CGTTTCAGCG AGGAATTCGA GGAACTGAAG 
CACGTTGGCC GGGATCAGCC GTGCACTTTT GCCGATCTAC CCTGCAATCG TCCGAAAAAC 
AGGTTCACCA ATATACTGCC CTACGATCAC TCACGTTTCA AGCTTCAGCC GGTGGACGAT 
GATGAGGGTA GTGATTATAT CAATGCCAAT TACGTGCCGG GTCACAATTC ACCGCGCGAG 
TTCATCGTGA CCCAGGGACC ATTGCATTCG ACACGCGATG ACTTCTGGCG AATGTGCTGG 
GAGAGCAACT CGCGGGCCAT AGTCATGCTG ACCAGGTGCT TTGAGAAGGG GCGCGAGAAG 
TGCGACCAGT ATTGGCCAAA TGATACGGTG CCCGTCTTCT ACGGTGACAT CAAGGTGCAG 
ATACTCAACG ACAGTCACTA TGCCGACTGG GTGATGACCG AGTTCATGCT ATGCAGAGGC 
AGCGAACAGC GCATCCTGCG ACACTTCCAC TTCACCACCT GGCCGGACTT CGGTGTTCCC 
AATCCGCCAC AGACACTGGT GCGCTTTGTG CGCGCATTCC GCGATCGAAT TGGTGCGGAA 
CAGCGACCCA TTGTGGTCCA TTGTAGCGCC GGTGTGGGAA GGTCGGGCAC CTTCATCACC 
CTGGATCGCA TCCTGCAACA GATCAACACG TCTGACTATG TGGACATATT TGGCATAGTA 
TATGCCATGC GCAAGGAGCG CGTTTGGATG GTGCAGACGG AGCAGCAGTA TATCTGCATC 
CACCAGTGCC TGCTGGCGGT GCTCGAGGGG AAGGAGAACA TCGTGGGTCC CGCTCGCGAG 
ATGCACGACA ACGAGGGCTA TGAAGGCCAA CAAGTGCAGC TGGACGAGAA CGGTGATGTG 
GTGGCCACCA TTGAGGGTCA CCTGTCGCAT CATGATCTGC AGCAGGCCGA GGCGGAGGCC 
ATTGACGATG AGAATGCGGC GATACTCCAT GATGACCAGC AGCCGCTGAC CAGTAGCTTT 
ACTGGACACC ACACTCACAT GCCGCCCACC ACATCGATGA GCTCATTTGG CGGCGGAGGT 
GGAGGCCATA CAAATGTGGA TGCACCGGAT AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727544.2
CDS	445..5340
Translation	MLYQLSKATTRIRLKRQKAVPQHRWLWSLAFLAAFTLKDVRCADLAISIPNNPGLDDGASYRLDYSPPFGYPEPNTTIASREIGDEIQFSRALPGTKYNFWLYYTNFTHHDWLTWTVTITTAPDPPSNLSVQVRSGKNAIILWSPPTQGSYTAFKIKVLGLSEASSSYNRTFQVNDNTFQHSVKELTPGATYQVQAYTIYDGKESVAYTSRNFTTKPNTPGKFIVWFRNETTLLVLWQPPYPAGIYTHYKVSIEPPDANDSVLYVEKEGEPPGPAQAAFKGLVPGRAYNISVQTMSEDEISLPTTAQYRTVPLRPLNVTFDRDFITSNSFRVLWEAPKGISEFDKYQVSVATTRRQSTVPRSNEPVAFFDFRDIAEPGKTFNVIVKTVSGKVTSWPATGDVTLRPLPVRNLRSINDDKTNTMIITWEADPASTQDEYRIVYHELETFNGDTSTLTTDRTRFTLESLLPGRNYSLSVQAVSKKMESNETSIFVVTRPSSPIIEDLKSIRMGLNISWKSDVNSKQEQYEVLYSRNGTSDLRTQKTKESRLVIKNLQPGAGYELKVFAVSHDLRSEPHAYFQAVYPNPPRNMTIETVRSNSVLVHWSPPESGEFTEYSIRYRTDSEQQWVRLPSVRSTEADITDMTKGEKYTIQVNTVSFGVESPVPQEVNTTVPPNPVSNIIQLVDSRNITLEWPKPEGRVESYILKWWPSDNPGRVQTKNVSENKSADDLSTVRVLIGELMPGVQYKFDIQTTSYGILSGITSLYPRTMPLIQSDVVVANGEKEDERDTITLSYTPTPQSSSKFDIYRFSLGDAEIRDKEKLANDTDRKVTFTGLVPGRLYNITVWTVSGGVASLPIQRQDRLYPEPITQLHATNITDTEISLRWDLPKGEYNDFDIAYLTADNLLAQNMTTRNEITISDLRPHRNYTFTVVVRSGTESSVLRSSSPLSASFTTNEAVPGRVERFHPTDVQPSEINFEWSLPSSEANGVIRQFSIAYTNINNLTDAGMQDFESEEAFGVIKNLKPGETYVFKIQAKTAIGFGPEREYRQTMPILAPPRPATQVVPTEVYRSSSTIQIRFRKNYFSDQNGQVRMYTIIVAEDDAKNASGLEMPSWLDVQSYSVWLPYQAIDPYYPFENRSVEDFTIGTENCDNHKIGYCNGPLKSGTTYRVKVRAFTGADKFTDTAYSFPIQTDQDNTSLIVAITVPLTIILVLLVTLLFYKRRRNNCRKTTKDSRANDNMSLPDSVIEQNRPILIKNFAEHYRLMSADSDFRFSEEFEELKHVGRDQPCTFADLPCNRPKNRFTNILPYDHSRFKLQPVDDDEGSDYINANYVPGHNSPREFIVTQGPLHSTRDDFWRMCWESNSRAIVMLTRCFEKGREKCDQYWPNDTVPVFYGDIKVQILNDSHYADWVMTEFMLCRGSEQRILRHFHFTTWPDFGVPNPPQTLVRFVRAFRDRIGAEQRPIVVHCSAGVGRSGTFITLDRILQQINTSDYVDIFGIVYAMRKERVWMVQTEQQYICIHQCLLAVLEGKENIVGPAREMHDNEGYEGQQVQLDENGDVVATIEGHLSHHDLQQAEAEAIDDENAAILHDDQQPLTSSFTGHHTHMPPTTSMSSFGGGGGGHTNVDAPDR

Target ORF information:

RefSeq Version	NM_167292.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant B (Ptp10D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167292.3

ORF Insert Sequence:

ATGTTGTACC AGCTAAGTAA AGCCACTACT AGAATCCGGC TCAAAAGGCA AAAAGCCGTT 
CCACAACATC GCTGGCTCTG GAGTTTAGCA TTTCTGGCAG CATTTACACT AAAGGACGTT 
CGATGTGCAG ATCTAGCCAT AAGTATACCC AATAATCCCG GCCTGGACGA TGGGGCCTCC 
TATCGACTGG ACTATAGTCC GCCCTTCGGT TATCCGGAGC CCAACACGAC GATTGCCTCG 
CGGGAAATCG GCGATGAGAT TCAATTCTCA CGCGCCCTAC CGGGCACCAA ATACAACTTT 
TGGCTGTACT ACACGAACTT TACGCACCAC GATTGGCTCA CCTGGACGGT GACGATAACA 
ACGGCTCCCG ATCCGCCGTC GAATCTGTCC GTTCAGGTGA GAAGCGGCAA GAATGCCATC 
ATCCTATGGT CACCGCCCAC CCAGGGCAGC TATACGGCGT TTAAGATCAA GGTGCTCGGA 
CTGTCGGAGG CGTCGAGCAG CTACAACCGC ACCTTTCAGG TGAACGACAA TACATTCCAG 
CACAGCGTCA AGGAGCTGAC ACCCGGAGCC ACCTACCAGG TGCAGGCATA TACCATCTAC 
GATGGCAAGG AGTCGGTGGC CTACACTAGT CGCAACTTCA CCACAAAGCC CAACACTCCG 
GGGAAATTCA TCGTCTGGTT CCGTAATGAG ACGACACTGC TGGTCCTGTG GCAGCCGCCA 
TATCCGGCGG GCATCTACAC GCACTACAAG GTATCCATCG AGCCGCCGGA TGCCAACGAT 
AGTGTGCTCT ATGTGGAAAA GGAGGGCGAA CCGCCCGGAC CGGCGCAGGC TGCCTTCAAG 
GGTCTGGTGC CCGGAAGGGC GTACAACATA TCCGTTCAGA CGATGTCCGA GGATGAGATC 
TCATTGCCGA CGACGGCGCA ATATCGAACG GTGCCGTTGC GTCCGCTGAA CGTGACCTTT 
GACCGTGACT TTATTACCTC CAATTCGTTC CGTGTCCTGT GGGAGGCGCC CAAGGGGATA 
TCCGAATTTG ACAAATACCA GGTATCGGTG GCCACAACAC GACGCCAATC CACAGTGCCG 
CGCAGCAATG AACCGGTGGC ATTCTTTGAT TTTCGCGACA TCGCCGAACC GGGCAAGACG 
TTCAATGTGA TCGTGAAGAC CGTATCCGGC AAGGTTACCT CGTGGCCAGC CACCGGGGAT 
GTGACACTGC GACCACTGCC CGTTCGCAAT TTGCGGAGCA TCAACGATGA CAAGACGAAT 
ACTATGATCA TAACGTGGGA AGCGGATCCG GCCAGCACGC AGGATGAGTA TCGCATTGTA 
TACCACGAAC TGGAGACATT TAATGGTGAC ACCAGTACCC TGACCACGGA TCGGACTCGA 
TTCACACTGG AGAGCCTGCT ACCCGGTCGC AACTACTCAT TGTCCGTCCA GGCGGTATCC 
AAGAAGATGG AATCGAATGA GACTAGCATC TTTGTGGTCA CCCGACCCTC GTCGCCCATC 
ATCGAGGACT TGAAGAGCAT ACGGATGGGT CTGAACATCA GTTGGAAGAG CGATGTCAAC 
TCCAAGCAGG AGCAGTACGA GGTGTTGTAC TCGCGCAACG GAACCAGCGA TTTGCGAACC 
CAAAAGACCA AAGAGTCGCG TCTGGTGATC AAGAATCTGC AGCCAGGTGC TGGCTATGAA 
CTCAAGGTGT TTGCAGTTAG TCACGATTTG CGCAGCGAAC CACATGCCTA TTTCCAAGCA 
GTTTATCCCA ATCCACCACG CAACATGACC ATCGAAACGG TGAGGAGTAA CTCGGTGCTG 
GTACACTGGT CACCGCCGGA AAGCGGTGAA TTTACCGAGT ACTCGATACG CTATCGCACG 
GACAGCGAAC AGCAGTGGGT GCGATTGCCC AGCGTTCGGT CCACGGAGGC GGATATCACC 
GATATGACCA AGGGCGAAAA GTACACCATC CAGGTGAACA CGGTCAGCTT TGGCGTAGAG 
AGTCCAGTGC CCCAGGAGGT GAACACGACG GTGCCGCCGA ATCCGGTGTC CAATATCATT 
CAACTGGTGG ACTCACGGAA CATTACGTTG GAGTGGCCCA AGCCAGAGGG TCGCGTGGAA 
TCGTACATTC TTAAGTGGTG GCCCAGCGAT AATCCCGGTC GTGTCCAGAC CAAGAATGTC 
TCCGAGAACA AGTCGGCCGA CGATTTGTCA ACAGTGCGTG TCCTCATCGG CGAACTGATG 
CCCGGCGTGC AGTACAAGTT TGACATCCAG ACGACGTCGT ATGGAATCCT GTCGGGCATC 
ACCAGTCTGT ATCCGCGCAC CATGCCGCTC ATCCAGTCGG ACGTGGTGGT GGCCAATGGC 
GAGAAGGAGG ACGAGAGGGA CACCATCACT CTGAGCTACA CACCCACACC GCAGTCGTCG 
TCCAAGTTCG ATATCTATCG ATTCTCTCTC GGCGATGCCG AGATCCGGGA CAAGGAGAAG 
CTGGCCAACG ATACGGATCG CAAGGTGACG TTTACGGGTC TGGTGCCTGG TCGATTGTAC 
AACATCACAG TTTGGACTGT GAGCGGTGGT GTGGCCAGTT TGCCCATACA ACGACAGGAT 
CGCCTGTATC CGGAACCCAT CACACAGCTG CATGCCACCA ACATCACGGA TACGGAGATC 
TCATTGCGCT GGGATTTGCC CAAGGGCGAG TACAATGACT TCGATATTGC CTATCTCACG 
GCGGACAATC TATTGGCCCA GAATATGACC ACCAGGAATG AGATCACCAT CAGTGACCTG 
CGACCCCACA GGAACTACAC CTTCACCGTG GTGGTACGTT CCGGCACTGA ATCGTCCGTG 
CTGAGGAGCA GTTCACCATT ATCCGCTAGC TTTACGACCA ATGAAGCGGT GCCCGGGCGA 
GTTGAACGCT TCCATCCCAC GGATGTTCAG CCCAGCGAGA TCAATTTCGA GTGGTCGCTG 
CCATCGAGTG AGGCAAATGG CGTTATTCGC CAGTTCTCGA TAGCCTATAC GAATATCAAT 
AATCTCACGG ACGCAGGCAT GCAGGACTTT GAGTCGGAGG AGGCATTCGG TGTGATCAAG 
AATCTAAAGC CCGGCGAGAC CTATGTGTTC AAGATTCAGG CCAAGACTGC CATCGGTTTC 
GGTCCGGAGC GGGAGTACCG TCAAACGATG CCGATATTAG CGCCACCACG TCCTGCCACC 
CAAGTGGTGC CCACCGAGGT CTATCGCAGC TCATCGACCA TCCAGATTCG GTTTAGGAAG 
AACTACTTCT CGGATCAAAA CGGCCAGGTG CGCATGTACA CGATCATCGT GGCCGAGGAT 
GATGCCAAGA ATGCATCCGG CCTGGAGATG CCCAGCTGGC TGGATGTGCA GTCGTACAGC 
GTTTGGTTGC CCTATCAGGC CATAGATCCG TACTATCCAT TCGAGAATCG ATCCGTAGAG 
GACTTCACCA TCGGTACGGA GAACTGTGAC AACCACAAGA TCGGCTACTG CAACGGACCA 
CTGAAATCGG GAACCACCTA TCGGGTTAAG GTGCGGGCGT TCACCGGAGC GGATAAGTTC 
ACGGATACCG CCTACAGTTT TCCCATTCAG ACAGATCAAG ACAACACCTC ACTGATTGTG 
GCCATTACGG TGCCGTTAAC TATCATCTTG GTGCTCCTGG TGACACTTTT GTTCTACAAA 
CGACGTCGCA ACAATTGCCG TAAGACGACC AAGGATTCGA GGGCCAACGA CAATATGTCC 
CTGCCGGATA GCGTAATCGA GCAGAATCGC CCCATTCTGA TCAAGAACTT TGCCGAGCAC 
TATCGCCTAA TGTCCGCCGA TTCGGACTTC CGTTTCAGCG AGGAATTCGA GGAACTGAAG 
CACGTTGGCC GGGATCAGCC GTGCACTTTT GCCGATCTAC CCTGCAATCG TCCGAAAAAC 
AGGTTCACCA ATATACTGCC CTACGATCAC TCACGTTTCA AGCTTCAGCC GGTGGACGAT 
GATGAGGGTA GTGATTATAT CAATGCCAAT TACGTGCCGG GTCACAATTC ACCGCGCGAG 
TTCATCGTGA CCCAGGGACC ATTGCATTCG ACACGCGATG ACTTCTGGCG AATGTGCTGG 
GAGAGCAACT CGCGGGCCAT AGTCATGCTG ACCAGGTGCT TTGAGAAGGG GCGCGAGAAG 
TGCGACCAGT ATTGGCCAAA TGATACGGTG CCCGTCTTCT ACGGTGACAT CAAGGTGCAG 
ATACTCAACG ACAGTCACTA TGCCGACTGG GTGATGACCG AGTTCATGCT ATGCAGAGGC 
AGCGAACAGC GCATCCTGCG ACACTTCCAC TTCACCACCT GGCCGGACTT CGGTGTTCCC 
AATCCGCCAC AGACACTGGT GCGCTTTGTG CGCGCATTCC GCGATCGAAT TGGTGCGGAA 
CAGCGACCCA TTGTGGTCCA TTGTAGCGCC GGTGTGGGAA GGTCGGGCAC CTTCATCACC 
CTGGATCGCA TCCTGCAACA GATCAACACG TCTGACTATG TGGACATATT TGGCATAGTA 
TATGCCATGC GCAAGGAGCG CGTTTGGATG GTGCAGACGG AGCAGCAGTA TATCTGCATC 
CACCAGTGCC TGCTGGCGGT GCTCGAGGGG AAGGAGAACA TCGTGGGTCC CGCTCGCGAG 
ATGCACGACA ACGAGGGCTA TGAAGGCCAA CAAGTGCAGC TGGACGAGAA CGGTGATGTG 
GTGGCCACCA TTGAGGGTCA CCTGTCGCAT CATGATCTGC AGCAGGCCGA GGCGGAGGCC 
ATTGACGATG AGAATGCGGC GATACTCCAT GATGACCAGC AGCCGCTGAC CAGTAGCTTT 
ACTGGACACC ACACTCACAT GCCGCCCACC ACATCGATGA GCTCATTTGG CGGCGGAGGT 
GGAGGCCATA CAAATGTGGA TGCACCGGAT AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptp10D ( NM_001272524.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259453.1 Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant H (Ptp10D), mRNA.

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CloneID	OFa02789
Clone ID Related Accession (Same CDS sequence)	NM_001272524.1
Accession Version	NM_001272524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein tyrosine phosphatase 10D, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGTACC AGCTAAGTAA AGCCACTACT AGAATCCGGC TCAAAAGGCA AAAAGCCGTT 
CCACAACATC GCTGGCTCTG GAGTTTAGCA TTTCTGGCAG CATTTACACT AAAGGACGTT 
CGATGTGCAG ATCTAGCCAT AAGTATACCC AATAATCCCG GCCTGGACGA TGGGGCCTCC 
TATCGACTGG ACTATAGTCC GCCCTTCGGT TATCCGGAGC CCAACACGAC GATTGCCTCG 
CGGGAAATCG GCGATGAGAT TCAATTCTCA CGCGCCCTAC CGGGCACCAA ATACAACTTT 
TGGCTGTACT ACACGAACTT TACGCACCAC GATTGGCTCA CCTGGACGGT GACGATAACA 
ACGGCTCCCG ATCCGCCGTC GAATCTGTCC GTTCAGGTGA GAAGCGGCAA GAATGCCATC 
ATCCTATGGT CACCGCCCAC CCAGGGCAGC TATACGGCGT TTAAGATCAA GGTGCTCGGA 
CTGTCGGAGG CGTCGAGCAG CTACAACCGC ACCTTTCAGG TGAACGACAA TACATTCCAG 
CACAGCGTCA AGGAGCTGAC ACCCGGAGCC ACCTACCAGG TGCAGGCATA TACCATCTAC 
GATGGCAAGG AGTCGGTGGC CTACACTAGT CGCAACTTCA CCACAAGTCG AAGGACGCGG 
CATAAAGAAT TGCTGGACAT TAAGATCCTA AGAGAGCCCA ACACTCCGGG GAAATTCATC 
GTCTGGTTCC GTAATGAGAC GACACTGCTG GTCCTGTGGC AGCCGCCATA TCCGGCGGGC 
ATCTACACGC ACTACAAGGT ATCCATCGAG CCGCCGGATG CCAACGATAG TGTGCTCTAT 
GTGGAAAAGG AGGGCGAACC GCCCGGACCG GCGCAGGCTG CCTTCAAGGG TCTGGTGCCC 
GGAAGGGCGT ACAACATATC CGTTCAGACG ATGTCCGAGG ATGAGATCTC ATTGCCGACG 
ACGGCGCAAT ATCGAACGGT GCCGTTGCGT CCGCTGAACG TGACCTTTGA CCGTGACTTT 
ATTACCTCCA ATTCGTTCCG TGTCCTGTGG GAGGCGCCCA AGGGGATATC CGAATTTGAC 
AAATACCAGG TATCGGTGGC CACAACACGA CGCCAATCCA CAGTGCCGCG CAGCAATGAA 
CCGGTGGCAT TCTTTGATTT TCGCGACATC GCCGAACCGG GCAAGACGTT CAATGTGATC 
GTGAAGACCG TATCCGGCAA GGTTACCTCG TGGCCAGCCA CCGGGGATGT GACACTGCGA 
CCACTGCCCG TTCGCAATTT GCGGAGCATC AACGATGACA AGACGAATAC TATGATCATA 
ACGTGGGAAG CGGATCCGGC CAGCACGCAG GATGAGTATC GCATTGTATA CCACGAACTG 
GAGACATTTA ATGGTGACAC CAGTACCCTG ACCACGGATC GGACTCGATT CACACTGGAG 
AGCCTGCTAC CCGGTCGCAA CTACTCATTG TCCGTCCAGG CGGTATCCAA GAAGATGGAA 
TCGAATGAGA CTAGCATCTT TGTGGTCACC CGACCCTCGT CGCCCATCAT CGAGGACTTG 
AAGAGCATAC GGATGGGTCT GAACATCAGT TGGAAGAGCG ATGTCAACTC CAAGCAGGAG 
CAGTACGAGG TGTTGTACTC GCGCAACGGA ACCAGCGATT TGCGAACCCA AAAGACCAAA 
GAGTCGCGTC TGGTGATCAA GAATCTGCAG CCAGGTGCTG GCTATGAACT CAAGGTGTTT 
GCAGTTAGTC ACGATTTGCG CAGCGAACCA CATGCCTATT TCCAAGCAGT TTATCCCAAT 
CCACCACGCA ACATGACCAT CGAAACGGTG AGGAGTAACT CGGTGCTGGT ACACTGGTCA 
CCGCCGGAAA GCGGTGAATT TACCGAGTAC TCGATACGCT ATCGCACGGA CAGCGAACAG 
CAGTGGGTGC GATTGCCCAG CGTTCGGTCC ACGGAGGCGG ATATCACCGA TATGACCAAG 
GGCGAAAAGT ACACCATCCA GGTGAACACG GTCAGCTTTG GCGTAGAGAG TCCAGTGCCC 
CAGGAGGTGA ACACGACGGT GCCGCCGAAT CCGGTGTCCA ATATCATTCA ACTGGTGGAC 
TCACGGAACA TTACGTTGGA GTGGCCCAAG CCAGAGGGTC GCGTGGAATC GTACATTCTT 
AAGTGGTGGC CCAGCGATAA TCCCGGTCGT GTCCAGACCA AGAATGTCTC CGAGAACAAG 
TCGGCCGACG ATTTGTCAAC AGTGCGTGTC CTCATCGGCG AACTGATGCC CGGCGTGCAG 
TACAAGTTTG ACATCCAGAC GACGTCGTAT GGAATCCTGT CGGGCATCAC CAGTCTGTAT 
CCGCGCACCA TGCCGCTCAT CCAGTCGGAC GTGGTGGTGG CCAATGGCGA GAAGGAGGAC 
GAGAGGGACA CCATCACTCT GAGCTACACA CCCACACCGC AGTCGTCGTC CAAGTTCGAT 
ATCTATCGAT TCTCTCTCGG CGATGCCGAG ATCCGGGACA AGGAGAAGCT GGCCAACGAT 
ACGGATCGCA AGGTGACGTT TACGGGTCTG GTGCCTGGTC GATTGTACAA CATCACAGTT 
TGGACTGTGA GCGGTGGTGT GGCCAGTTTG CCCATACAAC GACAGGATCG CCTGTATCCG 
GAACCCATCA CACAGCTGCA TGCCACCAAC ATCACGGATA CGGAGATCTC ATTGCGCTGG 
GATTTGCCCA AGGGCGAGTA CAATGACTTC GATATTGCCT ATCTCACGGC GGACAATCTA 
TTGGCCCAGA ATATGACCAC CAGGAATGAG ATCACCATCA GTGACCTGCG ACCCCACAGG 
AACTACACCT TCACCGTGGT GGTACGTTCC GGCACTGAAT CGTCCGTGCT GAGGAGCAGT 
TCACCATTAT CCGCTAGCTT TACGACCAAT GAAGCGGTGC CCGGGCGAGT TGAACGCTTC 
CATCCCACGG ATGTTCAGCC CAGCGAGATC AATTTCGAGT GGTCGCTGCC ATCGAGTGAG 
GCAAATGGCG TTATTCGCCA GTTCTCGATA GCCTATACGA ATATCAATAA TCTCACGGAC 
GCAGGCATGC AGGACTTTGA GTCGGAGGAG GCATTCGGTG TGATCAAGAA TCTAAAGCCC 
GGCGAGACCT ATGTGTTCAA GATTCAGGCC AAGACTGCCA TCGGTTTCGG TCCGGAGCGG 
GAGTACCGTC AAACGATGCC GATATTAGCG CCACCACGTC CTGCCACCCA AGTGGTGCCC 
ACCGAGGTCT ATCGCAGCTC ATCGACCATC CAGATTCGGT TTAGGAAGAA CTACTTCTCG 
GATCAAAACG GCCAGGTGCG CATGTACACG ATCATCGTGG CCGAGGATGA TGCCAAGAAT 
GCATCCGGCC TGGAGATGCC CAGCTGGCTG GATGTGCAGT CGTACAGCGT TTGGTTGCCC 
TATCAGGCCA TAGATCCGTA CTATCCATTC GAGAATCGAT CCGTAGAGGA CTTCACCATC 
GGTACGGAGA ACTGTGACAA CCACAAGATC GGCTACTGCA ACGGACCACT GAAATCGGGA 
ACCACCTATC GGGTTAAGGT GCGGGCGTTC ACCGGAGCGG ATAAGTTCAC GGATACCGCC 
TACAGTTTTC CCATTCAGAC AGAAATGCTA AGTTCGCCGG ATCAAGACAA CACCTCACTG 
ATTGTGGCCA TTACGGTGCC GTTAACTATC ATCTTGGTGC TCCTGGTGAC ACTTTTGTTC 
TACAAACGAC GTCGCAACAA TTGCCGTAAG ACGACCAAGG ATTCGAGGGC CAACGACAAT 
ATGTCCCTGC CGGATAGCGT AATCGAGCAG AATCGCCCCA TTCTGATCAA GAACTTTGCC 
GAGCACTATC GCCTAATGTC CGCCGATTCG GACTTCCGTT TCAGCGAGGA ATTCGAGGAA 
CTGAAGCACG TTGGCCGGGA TCAGCCGTGC ACTTTTGCCG ATCTACCCTG CAATCGTCCG 
AAAAACAGGT TCACCAATAT ACTGCCCTAC GATCACTCAC GTTTCAAGCT TCAGCCGGTG 
GACGATGATG AGGGTAGTGA TTATATCAAT GCCAATTACG TGCCGGGTCA CAATTCACCG 
CGCGAGTTCA TCGTGACCCA GGGACCATTG CATTCGACAC GCGATGACTT CTGGCGAATG 
TGCTGGGAGA GCAACTCGCG GGCCATAGTC ATGCTGACCA GGTGCTTTGA GAAGGGGCGC 
GAGAAGTGCG ACCAGTATTG GCCAAATGAT ACGGTGCCCG TCTTCTACGG TGACATCAAG 
GTGCAGATAC TCAACGACAG TCACTATGCC GACTGGGTGA TGACCGAGTT CATGCTATGC 
AGAGGCAGCG AACAGCGCAT CCTGCGACAC TTCCACTTCA CCACCTGGCC GGACTTCGGT 
GTTCCCAATC CGCCACAGAC ACTGGTGCGC TTTGTGCGCG CATTCCGCGA TCGAATTGGT 
GCGGAACAGC GACCCATTGT GGTCCATTGT AGCGCCGGTG TGGGAAGGTC GGGCACCTTC 
ATCACCCTGG ATCGCATCCT GCAACAGATC AACACGTCTG ACTATGTGGA CATATTTGGC 
ATAGTATATG CCATGCGCAA GGAGCGCGTT TGGATGGTGC AGACGGAGCA GCAGTATATC 
TGCATCCACC AGTGCCTGCT GGCGGTGCTC GAGGGGAAGG AGAACATCGT GGGTCCCGCT 
CGCGAGATGC ACGACAACGA GGGCTATGAA GGCCAACAAG TGCAGCTGGA CGAGAACGGT 
GATGTGGTGG CCACCATTGA GGGTCACCTG TCGCATCATG ATCTGCAGCA GGCCGAGGCG 
GAGGCCATTG ACGATGAGAA TGCGGCGATA CTCCATGATG ACCAGCAGCC GCTGACCAGT 
AGCTTTACTG GACACCACAC TCACATGCCG CCCACCACAT CGATGAGCTC ATTTGGCGGC 
GGAGGTGGAG GCCATACAAA TGTGGATGCA CCGGATAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259453.1
CDS	445..5406
Translation	MLYQLSKATTRIRLKRQKAVPQHRWLWSLAFLAAFTLKDVRCADLAISIPNNPGLDDGASYRLDYSPPFGYPEPNTTIASREIGDEIQFSRALPGTKYNFWLYYTNFTHHDWLTWTVTITTAPDPPSNLSVQVRSGKNAIILWSPPTQGSYTAFKIKVLGLSEASSSYNRTFQVNDNTFQHSVKELTPGATYQVQAYTIYDGKESVAYTSRNFTTSRRTRHKELLDIKILREPNTPGKFIVWFRNETTLLVLWQPPYPAGIYTHYKVSIEPPDANDSVLYVEKEGEPPGPAQAAFKGLVPGRAYNISVQTMSEDEISLPTTAQYRTVPLRPLNVTFDRDFITSNSFRVLWEAPKGISEFDKYQVSVATTRRQSTVPRSNEPVAFFDFRDIAEPGKTFNVIVKTVSGKVTSWPATGDVTLRPLPVRNLRSINDDKTNTMIITWEADPASTQDEYRIVYHELETFNGDTSTLTTDRTRFTLESLLPGRNYSLSVQAVSKKMESNETSIFVVTRPSSPIIEDLKSIRMGLNISWKSDVNSKQEQYEVLYSRNGTSDLRTQKTKESRLVIKNLQPGAGYELKVFAVSHDLRSEPHAYFQAVYPNPPRNMTIETVRSNSVLVHWSPPESGEFTEYSIRYRTDSEQQWVRLPSVRSTEADITDMTKGEKYTIQVNTVSFGVESPVPQEVNTTVPPNPVSNIIQLVDSRNITLEWPKPEGRVESYILKWWPSDNPGRVQTKNVSENKSADDLSTVRVLIGELMPGVQYKFDIQTTSYGILSGITSLYPRTMPLIQSDVVVANGEKEDERDTITLSYTPTPQSSSKFDIYRFSLGDAEIRDKEKLANDTDRKVTFTGLVPGRLYNITVWTVSGGVASLPIQRQDRLYPEPITQLHATNITDTEISLRWDLPKGEYNDFDIAYLTADNLLAQNMTTRNEITISDLRPHRNYTFTVVVRSGTESSVLRSSSPLSASFTTNEAVPGRVERFHPTDVQPSEINFEWSLPSSEANGVIRQFSIAYTNINNLTDAGMQDFESEEAFGVIKNLKPGETYVFKIQAKTAIGFGPEREYRQTMPILAPPRPATQVVPTEVYRSSSTIQIRFRKNYFSDQNGQVRMYTIIVAEDDAKNASGLEMPSWLDVQSYSVWLPYQAIDPYYPFENRSVEDFTIGTENCDNHKIGYCNGPLKSGTTYRVKVRAFTGADKFTDTAYSFPIQTEMLSSPDQDNTSLIVAITVPLTIILVLLVTLLFYKRRRNNCRKTTKDSRANDNMSLPDSVIEQNRPILIKNFAEHYRLMSADSDFRFSEEFEELKHVGRDQPCTFADLPCNRPKNRFTNILPYDHSRFKLQPVDDDEGSDYINANYVPGHNSPREFIVTQGPLHSTRDDFWRMCWESNSRAIVMLTRCFEKGREKCDQYWPNDTVPVFYGDIKVQILNDSHYADWVMTEFMLCRGSEQRILRHFHFTTWPDFGVPNPPQTLVRFVRAFRDRIGAEQRPIVVHCSAGVGRSGTFITLDRILQQINTSDYVDIFGIVYAMRKERVWMVQTEQQYICIHQCLLAVLEGKENIVGPAREMHDNEGYEGQQVQLDENGDVVATIEGHLSHHDLQQAEAEAIDDENAAILHDDQQPLTSSFTGHHTHMPPTTSMSSFGGGGGGHTNVDAPDR

Target ORF information:

RefSeq Version	NM_001272524.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein tyrosine phosphatase 10D, transcript variant H (Ptp10D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272524.1

ORF Insert Sequence:

ATGTTGTACC AGCTAAGTAA AGCCACTACT AGAATCCGGC TCAAAAGGCA AAAAGCCGTT 
CCACAACATC GCTGGCTCTG GAGTTTAGCA TTTCTGGCAG CATTTACACT AAAGGACGTT 
CGATGTGCAG ATCTAGCCAT AAGTATACCC AATAATCCCG GCCTGGACGA TGGGGCCTCC 
TATCGACTGG ACTATAGTCC GCCCTTCGGT TATCCGGAGC CCAACACGAC GATTGCCTCG 
CGGGAAATCG GCGATGAGAT TCAATTCTCA CGCGCCCTAC CGGGCACCAA ATACAACTTT 
TGGCTGTACT ACACGAACTT TACGCACCAC GATTGGCTCA CCTGGACGGT GACGATAACA 
ACGGCTCCCG ATCCGCCGTC GAATCTGTCC GTTCAGGTGA GAAGCGGCAA GAATGCCATC 
ATCCTATGGT CACCGCCCAC CCAGGGCAGC TATACGGCGT TTAAGATCAA GGTGCTCGGA 
CTGTCGGAGG CGTCGAGCAG CTACAACCGC ACCTTTCAGG TGAACGACAA TACATTCCAG 
CACAGCGTCA AGGAGCTGAC ACCCGGAGCC ACCTACCAGG TGCAGGCATA TACCATCTAC 
GATGGCAAGG AGTCGGTGGC CTACACTAGT CGCAACTTCA CCACAAGTCG AAGGACGCGG 
CATAAAGAAT TGCTGGACAT TAAGATCCTA AGAGAGCCCA ACACTCCGGG GAAATTCATC 
GTCTGGTTCC GTAATGAGAC GACACTGCTG GTCCTGTGGC AGCCGCCATA TCCGGCGGGC 
ATCTACACGC ACTACAAGGT ATCCATCGAG CCGCCGGATG CCAACGATAG TGTGCTCTAT 
GTGGAAAAGG AGGGCGAACC GCCCGGACCG GCGCAGGCTG CCTTCAAGGG TCTGGTGCCC 
GGAAGGGCGT ACAACATATC CGTTCAGACG ATGTCCGAGG ATGAGATCTC ATTGCCGACG 
ACGGCGCAAT ATCGAACGGT GCCGTTGCGT CCGCTGAACG TGACCTTTGA CCGTGACTTT 
ATTACCTCCA ATTCGTTCCG TGTCCTGTGG GAGGCGCCCA AGGGGATATC CGAATTTGAC 
AAATACCAGG TATCGGTGGC CACAACACGA CGCCAATCCA CAGTGCCGCG CAGCAATGAA 
CCGGTGGCAT TCTTTGATTT TCGCGACATC GCCGAACCGG GCAAGACGTT CAATGTGATC 
GTGAAGACCG TATCCGGCAA GGTTACCTCG TGGCCAGCCA CCGGGGATGT GACACTGCGA 
CCACTGCCCG TTCGCAATTT GCGGAGCATC AACGATGACA AGACGAATAC TATGATCATA 
ACGTGGGAAG CGGATCCGGC CAGCACGCAG GATGAGTATC GCATTGTATA CCACGAACTG 
GAGACATTTA ATGGTGACAC CAGTACCCTG ACCACGGATC GGACTCGATT CACACTGGAG 
AGCCTGCTAC CCGGTCGCAA CTACTCATTG TCCGTCCAGG CGGTATCCAA GAAGATGGAA 
TCGAATGAGA CTAGCATCTT TGTGGTCACC CGACCCTCGT CGCCCATCAT CGAGGACTTG 
AAGAGCATAC GGATGGGTCT GAACATCAGT TGGAAGAGCG ATGTCAACTC CAAGCAGGAG 
CAGTACGAGG TGTTGTACTC GCGCAACGGA ACCAGCGATT TGCGAACCCA AAAGACCAAA 
GAGTCGCGTC TGGTGATCAA GAATCTGCAG CCAGGTGCTG GCTATGAACT CAAGGTGTTT 
GCAGTTAGTC ACGATTTGCG CAGCGAACCA CATGCCTATT TCCAAGCAGT TTATCCCAAT 
CCACCACGCA ACATGACCAT CGAAACGGTG AGGAGTAACT CGGTGCTGGT ACACTGGTCA 
CCGCCGGAAA GCGGTGAATT TACCGAGTAC TCGATACGCT ATCGCACGGA CAGCGAACAG 
CAGTGGGTGC GATTGCCCAG CGTTCGGTCC ACGGAGGCGG ATATCACCGA TATGACCAAG 
GGCGAAAAGT ACACCATCCA GGTGAACACG GTCAGCTTTG GCGTAGAGAG TCCAGTGCCC 
CAGGAGGTGA ACACGACGGT GCCGCCGAAT CCGGTGTCCA ATATCATTCA ACTGGTGGAC 
TCACGGAACA TTACGTTGGA GTGGCCCAAG CCAGAGGGTC GCGTGGAATC GTACATTCTT 
AAGTGGTGGC CCAGCGATAA TCCCGGTCGT GTCCAGACCA AGAATGTCTC CGAGAACAAG 
TCGGCCGACG ATTTGTCAAC AGTGCGTGTC CTCATCGGCG AACTGATGCC CGGCGTGCAG 
TACAAGTTTG ACATCCAGAC GACGTCGTAT GGAATCCTGT CGGGCATCAC CAGTCTGTAT 
CCGCGCACCA TGCCGCTCAT CCAGTCGGAC GTGGTGGTGG CCAATGGCGA GAAGGAGGAC 
GAGAGGGACA CCATCACTCT GAGCTACACA CCCACACCGC AGTCGTCGTC CAAGTTCGAT 
ATCTATCGAT TCTCTCTCGG CGATGCCGAG ATCCGGGACA AGGAGAAGCT GGCCAACGAT 
ACGGATCGCA AGGTGACGTT TACGGGTCTG GTGCCTGGTC GATTGTACAA CATCACAGTT 
TGGACTGTGA GCGGTGGTGT GGCCAGTTTG CCCATACAAC GACAGGATCG CCTGTATCCG 
GAACCCATCA CACAGCTGCA TGCCACCAAC ATCACGGATA CGGAGATCTC ATTGCGCTGG 
GATTTGCCCA AGGGCGAGTA CAATGACTTC GATATTGCCT ATCTCACGGC GGACAATCTA 
TTGGCCCAGA ATATGACCAC CAGGAATGAG ATCACCATCA GTGACCTGCG ACCCCACAGG 
AACTACACCT TCACCGTGGT GGTACGTTCC GGCACTGAAT CGTCCGTGCT GAGGAGCAGT 
TCACCATTAT CCGCTAGCTT TACGACCAAT GAAGCGGTGC CCGGGCGAGT TGAACGCTTC 
CATCCCACGG ATGTTCAGCC CAGCGAGATC AATTTCGAGT GGTCGCTGCC ATCGAGTGAG 
GCAAATGGCG TTATTCGCCA GTTCTCGATA GCCTATACGA ATATCAATAA TCTCACGGAC 
GCAGGCATGC AGGACTTTGA GTCGGAGGAG GCATTCGGTG TGATCAAGAA TCTAAAGCCC 
GGCGAGACCT ATGTGTTCAA GATTCAGGCC AAGACTGCCA TCGGTTTCGG TCCGGAGCGG 
GAGTACCGTC AAACGATGCC GATATTAGCG CCACCACGTC CTGCCACCCA AGTGGTGCCC 
ACCGAGGTCT ATCGCAGCTC ATCGACCATC CAGATTCGGT TTAGGAAGAA CTACTTCTCG 
GATCAAAACG GCCAGGTGCG CATGTACACG ATCATCGTGG CCGAGGATGA TGCCAAGAAT 
GCATCCGGCC TGGAGATGCC CAGCTGGCTG GATGTGCAGT CGTACAGCGT TTGGTTGCCC 
TATCAGGCCA TAGATCCGTA CTATCCATTC GAGAATCGAT CCGTAGAGGA CTTCACCATC 
GGTACGGAGA ACTGTGACAA CCACAAGATC GGCTACTGCA ACGGACCACT GAAATCGGGA 
ACCACCTATC GGGTTAAGGT GCGGGCGTTC ACCGGAGCGG ATAAGTTCAC GGATACCGCC 
TACAGTTTTC CCATTCAGAC AGAAATGCTA AGTTCGCCGG ATCAAGACAA CACCTCACTG 
ATTGTGGCCA TTACGGTGCC GTTAACTATC ATCTTGGTGC TCCTGGTGAC ACTTTTGTTC 
TACAAACGAC GTCGCAACAA TTGCCGTAAG ACGACCAAGG ATTCGAGGGC CAACGACAAT 
ATGTCCCTGC CGGATAGCGT AATCGAGCAG AATCGCCCCA TTCTGATCAA GAACTTTGCC 
GAGCACTATC GCCTAATGTC CGCCGATTCG GACTTCCGTT TCAGCGAGGA ATTCGAGGAA 
CTGAAGCACG TTGGCCGGGA TCAGCCGTGC ACTTTTGCCG ATCTACCCTG CAATCGTCCG 
AAAAACAGGT TCACCAATAT ACTGCCCTAC GATCACTCAC GTTTCAAGCT TCAGCCGGTG 
GACGATGATG AGGGTAGTGA TTATATCAAT GCCAATTACG TGCCGGGTCA CAATTCACCG 
CGCGAGTTCA TCGTGACCCA GGGACCATTG CATTCGACAC GCGATGACTT CTGGCGAATG 
TGCTGGGAGA GCAACTCGCG GGCCATAGTC ATGCTGACCA GGTGCTTTGA GAAGGGGCGC 
GAGAAGTGCG ACCAGTATTG GCCAAATGAT ACGGTGCCCG TCTTCTACGG TGACATCAAG 
GTGCAGATAC TCAACGACAG TCACTATGCC GACTGGGTGA TGACCGAGTT CATGCTATGC 
AGAGGCAGCG AACAGCGCAT CCTGCGACAC TTCCACTTCA CCACCTGGCC GGACTTCGGT 
GTTCCCAATC CGCCACAGAC ACTGGTGCGC TTTGTGCGCG CATTCCGCGA TCGAATTGGT 
GCGGAACAGC GACCCATTGT GGTCCATTGT AGCGCCGGTG TGGGAAGGTC GGGCACCTTC 
ATCACCCTGG ATCGCATCCT GCAACAGATC AACACGTCTG ACTATGTGGA CATATTTGGC 
ATAGTATATG CCATGCGCAA GGAGCGCGTT TGGATGGTGC AGACGGAGCA GCAGTATATC 
TGCATCCACC AGTGCCTGCT GGCGGTGCTC GAGGGGAAGG AGAACATCGT GGGTCCCGCT 
CGCGAGATGC ACGACAACGA GGGCTATGAA GGCCAACAAG TGCAGCTGGA CGAGAACGGT 
GATGTGGTGG CCACCATTGA GGGTCACCTG TCGCATCATG ATCTGCAGCA GGCCGAGGCG 
GAGGCCATTG ACGATGAGAA TGCGGCGATA CTCCATGATG ACCAGCAGCC GCTGACCAGT 
AGCTTTACTG GACACCACAC TCACATGCCG CCCACCACAT CGATGAGCTC ATTTGGCGGC 
GGAGGTGGAG GCCATACAAA TGTGGATGCA CCGGATAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ptp10D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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