Met cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Met
Entrez Gene ID	32114
Full Name	Methoprene-tolerant
Synonyms	CG1705,DmMet,Dmel\CG1705,MET,Met/Met1,Mett,Rst(1)JH,bHLHe59,met
General protein information	Preferred Names Methoprene-tolerant Names CG1705 gene product from transcript CG1705-RB CG1705-PA CG1705-PB Met-PA Met-PB Methoprene-tolerant protein juvenile hormone resistance methoprene tolerant methoprene-tolerant resistance (1) juvenile H resistance to juvenile hormone
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10D1-10D1\|1-35.4 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298203.1	NP_001285132.1	Methoprene-tolerant, isoform B
NM_078571.4	NP_511126.2	Methoprene-tolerant, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nup358 facilitates JH-induced Met nuclear transport in a manner dependent on importin beta and Hsp83.
Title: Nucleoporin Nup358 facilitates nuclear import of Methoprene-tolerant (Met) in an importin ?- and Hsp83-dependent manner.

the age-related sensitization of Or47b neurons requires a reproductive hormone, juvenile hormone, as well as its binding protein Methoprene-tolerant.
Title: Hormonal Modulation of Pheromone Detection Enhances Male Courtship Success.

genetic evidence definitively establishes Gce/Met in a Juvenile hormone receptor role, thus resolving a long-standing question in arthropod biology.
Title: Genetic Evidence for Function of the bHLH-PAS Protein Gce/Met As a Juvenile Hormone Receptor.

MF is produced by the larval CA and released into the hemolymph, from where it exerts its anti-metamorphic effects indirectly after conversion to JHB3, as well as acting as a hormone itself through the two JH receptors, Met and Gce.
Title: Methyl farnesoate plays a dual role in regulating Drosophila metamorphosis.

a novel NR box enables MET and GCE to interact JH-dependently with the AF2 of FTZ-F1.
Title: The Drosophila juvenile hormone receptor candidates methoprene-tolerant (MET) and germ cell-expressed (GCE) utilize a conserved LIXXL motif to bind the FTZ-F1 nuclear receptor.

The following Met gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Met cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01301	NM_078571.4 Latest version!	Drosophila melanogaster Methoprene-tolerant (Met), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01301	NM_001298203.1 Latest version!	Drosophila melanogaster Methoprene-tolerant (Met), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Met ( NM_001298203.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285132.1 Drosophila melanogaster Methoprene-tolerant (Met), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01301
Clone ID Related Accession (Same CDS sequence)	NM_001298203.1 , NM_078571.4
Accession Version	NM_001298203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Methoprene-tolerant, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAC CAGAGACGGG CAACACGGGC TCCACAGGAT CCGCTGGCTC GACAGGATCG 
GGATCGGGAT CGGGATCGGG AAGTGGGAGC TCCTCAGATC CAGCGAATGG ACGGGAGGCC 
CGTAACCTTG CCGAAAAACA GCGACGGGAT AAGCTTAATG CCAGCATCCA GGAGCTGGCC 
ACCATGGTAC CACATGCAGC CGAATCCTCC CGTCGCCTGG ACAAAACCGC CGTCCTTAGA 
TTCGCCACCC ATGGCCTGAG ACTTCAGTAT GTCTTTGGCA AGTCCGCTTC CAGACGTCGC 
AAGAAAACCG GCCTCAAGGG AACGGGTATG TCTGCCTCAC CTGTCGGAGA TCTACCCAAT 
CCCAGTCTGC ATCTAACGGA CACTCTAATG CAACTGCTGG ACTGCTGCTT CCTCACCCTA 
ACCTGCAGTG GCCAAATCGT TTTGGTATCC ACCAGCGTGG AGCAGCTATT GGGTCACTGT 
CAGTCCGATT TGTATGGCCA GAATCTACTG CAGATCACGC ATCCCGATGA TCAGGATCTG 
TTAAGACAGC AGCTAATACC CAGGGATATA GAGACCCTGT TCTATCAGCA TCAGCACCAC 
CAGCAGCAGG GGCACAATCC CCAGCAGCAC TCCACTTCCA CGTCGGCCTC AACTTCGGGC 
AGTGATCTGG AGGAGGAGGA AATGGAGACG GAGGAACACC GTCTGGGTCG GCAGCAGGGA 
GAGGCGGACG ATGACGAGGA TCACCCGTAC AACCGACGAA CACCCAGCCC GCGGAGAATG 
GCCCATTTGG CGACCATTGA TGACCGACTA CGCATGGATC GGCGCTGCTT TACCGTCCGC 
TTGGCTAGGG CTTCCACGCG AGCGGAGGCC ACGCGTCATT ACGAGCGGGT TAAGATCGAT 
GGCTGCTTTC GTCGCAGTGA CTCCTCCTTA ACCGGAGGTG CCGCTGCCAA CTATCCGATT 
GTCTCCCAGC TGATACGACG CTCGAGAAAC AACAATATGC TGGCTGCTGC TGCAGCAGTG 
GCAGCAGAAG CGGCGACGGT GCCGCCCCAG CACGATGCCA TTGCCCAGGC GGCGCTGCAC 
GGGATTAGCG GCAATGATAT TGTCCTGGTG GCCATGGCCA GGGTGCTGCG AGAGGAACGG 
CCGCCTGAGG AGACGGAGGG TACAGTGGGC TTGACCATTT ACAGACAGCC AGAACCCTAT 
CAGTTGGAGT ACCATACGAG GCATCTAATC GACGGCAGCA TCATCGACTG TGATCAAAGG 
ATTGGTCTGG TGGCGGGATA TATGAAGGAT GAGGTGCGCA ACCTTAGTCC CTTCTGTTTC 
ATGCACCTGG ACGACGTTCG CTGGGTGATT GTGGCCCTTC GACAAATGTA CGATTGCAAC 
AGTGACTACG GCGAGAGCTG CTACCGTCTG CTGTCCCGCA ACGGGCGCTT CATTTACCTG 
CACACCAAGG GATTTCTGGA GGTCGACCGT GGCAGTAATA AGGTGCATTC CTTTCTGTGC 
GTCAACACGC TGCTCGATGA GGAGGCGGGC CGGCAAAAGG TGCAGGAGAT GAAGGAGAAA 
TTCTCGACAA TCATCAAGGC GGAGATGCCC ACGCAGAGCA GCAGTCCCGA TTTGCCCGCC 
TCGCAGGCAC CGCAGCAACT TGAGAGAATT GTCCTCTATC TAATAGAGAA CCTACAGAAG 
AGTGTGGATT CAGCAGAGAC GGTTGGCGGC CAGGGCATGG AAAGCCTAAT GGACGATGGC 
TACAGTTCGC CAGCAAATAC CTTAACTCTC GAGGAGTTAG CTCCCTCGCC CACGCCCGCC 
TTGGCCTTGG TGCCGCCGGC TCCCTCATCG GTCAAGAGCT CCATCTCCAA GTCGGTGAGT 
GTGGTCAATG TGACGGCGGC CAGAAAGTTT CAGCAGGAGC ATCAGAAGCA GCGTGAACGT 
GACCGTGAGC AGCTTAAGGA GCGCACCAAC TCCACGCAGG GCGTGATCCG GCAACTGAGC 
AGCTGCCTAA GCGAGGCGGA AACGGCATCC TGTATCCTAT CACCAGCCAG TAGCTTGAGT 
GCCAGCGAAG CACCGGACAC GCCCGATCCG CACAGCAACA CATCACCGCC ACCGTCGCTC 
CACACACGTC CCAGTGTCCT GCATCGAACC CTGACCAGCA CGCTGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285132.1
CDS	745..2895
Translation	MAAPETGNTGSTGSAGSTGSGSGSGSGSGSSSDPANGREARNLAEKQRRDKLNASIQELATMVPHAAESSRRLDKTAVLRFATHGLRLQYVFGKSASRRRKKTGLKGTGMSASPVGDLPNPSLHLTDTLMQLLDCCFLTLTCSGQIVLVSTSVEQLLGHCQSDLYGQNLLQITHPDDQDLLRQQLIPRDIETLFYQHQHHQQQGHNPQQHSTSTSASTSGSDLEEEEMETEEHRLGRQQGEADDDEDHPYNRRTPSPRRMAHLATIDDRLRMDRRCFTVRLARASTRAEATRHYERVKIDGCFRRSDSSLTGGAAANYPIVSQLIRRSRNNNMLAAAAAVAAEAATVPPQHDAIAQAALHGISGNDIVLVAMARVLREERPPEETEGTVGLTIYRQPEPYQLEYHTRHLIDGSIIDCDQRIGLVAGYMKDEVRNLSPFCFMHLDDVRWVIVALRQMYDCNSDYGESCYRLLSRNGRFIYLHTKGFLEVDRGSNKVHSFLCVNTLLDEEAGRQKVQEMKEKFSTIIKAEMPTQSSSPDLPASQAPQQLERIVLYLIENLQKSVDSAETVGGQGMESLMDDGYSSPANTLTLEELAPSPTPALALVPPAPSSVKSSISKSVSVVNVTAARKFQQEHQKQRERDREQLKERTNSTQGVIRQLSSCLSEAETASCILSPASSLSASEAPDTPDPHSNTSPPPSLHTRPSVLHRTLTSTLR

Target ORF information:

RefSeq Version	NM_001298203.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Methoprene-tolerant (Met), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298203.1

ORF Insert Sequence:

ATGGCAGCAC CAGAGACGGG CAACACGGGC TCCACAGGAT CCGCTGGCTC GACAGGATCG 
GGATCGGGAT CGGGATCGGG AAGTGGGAGC TCCTCAGATC CAGCGAATGG ACGGGAGGCC 
CGTAACCTTG CCGAAAAACA GCGACGGGAT AAGCTTAATG CCAGCATCCA GGAGCTGGCC 
ACCATGGTAC CACATGCAGC CGAATCCTCC CGTCGCCTGG ACAAAACCGC CGTCCTTAGA 
TTCGCCACCC ATGGCCTGAG ACTTCAGTAT GTCTTTGGCA AGTCCGCTTC CAGACGTCGC 
AAGAAAACCG GCCTCAAGGG AACGGGTATG TCTGCCTCAC CTGTCGGAGA TCTACCCAAT 
CCCAGTCTGC ATCTAACGGA CACTCTAATG CAACTGCTGG ACTGCTGCTT CCTCACCCTA 
ACCTGCAGTG GCCAAATCGT TTTGGTATCC ACCAGCGTGG AGCAGCTATT GGGTCACTGT 
CAGTCCGATT TGTATGGCCA GAATCTACTG CAGATCACGC ATCCCGATGA TCAGGATCTG 
TTAAGACAGC AGCTAATACC CAGGGATATA GAGACCCTGT TCTATCAGCA TCAGCACCAC 
CAGCAGCAGG GGCACAATCC CCAGCAGCAC TCCACTTCCA CGTCGGCCTC AACTTCGGGC 
AGTGATCTGG AGGAGGAGGA AATGGAGACG GAGGAACACC GTCTGGGTCG GCAGCAGGGA 
GAGGCGGACG ATGACGAGGA TCACCCGTAC AACCGACGAA CACCCAGCCC GCGGAGAATG 
GCCCATTTGG CGACCATTGA TGACCGACTA CGCATGGATC GGCGCTGCTT TACCGTCCGC 
TTGGCTAGGG CTTCCACGCG AGCGGAGGCC ACGCGTCATT ACGAGCGGGT TAAGATCGAT 
GGCTGCTTTC GTCGCAGTGA CTCCTCCTTA ACCGGAGGTG CCGCTGCCAA CTATCCGATT 
GTCTCCCAGC TGATACGACG CTCGAGAAAC AACAATATGC TGGCTGCTGC TGCAGCAGTG 
GCAGCAGAAG CGGCGACGGT GCCGCCCCAG CACGATGCCA TTGCCCAGGC GGCGCTGCAC 
GGGATTAGCG GCAATGATAT TGTCCTGGTG GCCATGGCCA GGGTGCTGCG AGAGGAACGG 
CCGCCTGAGG AGACGGAGGG TACAGTGGGC TTGACCATTT ACAGACAGCC AGAACCCTAT 
CAGTTGGAGT ACCATACGAG GCATCTAATC GACGGCAGCA TCATCGACTG TGATCAAAGG 
ATTGGTCTGG TGGCGGGATA TATGAAGGAT GAGGTGCGCA ACCTTAGTCC CTTCTGTTTC 
ATGCACCTGG ACGACGTTCG CTGGGTGATT GTGGCCCTTC GACAAATGTA CGATTGCAAC 
AGTGACTACG GCGAGAGCTG CTACCGTCTG CTGTCCCGCA ACGGGCGCTT CATTTACCTG 
CACACCAAGG GATTTCTGGA GGTCGACCGT GGCAGTAATA AGGTGCATTC CTTTCTGTGC 
GTCAACACGC TGCTCGATGA GGAGGCGGGC CGGCAAAAGG TGCAGGAGAT GAAGGAGAAA 
TTCTCGACAA TCATCAAGGC GGAGATGCCC ACGCAGAGCA GCAGTCCCGA TTTGCCCGCC 
TCGCAGGCAC CGCAGCAACT TGAGAGAATT GTCCTCTATC TAATAGAGAA CCTACAGAAG 
AGTGTGGATT CAGCAGAGAC GGTTGGCGGC CAGGGCATGG AAAGCCTAAT GGACGATGGC 
TACAGTTCGC CAGCAAATAC CTTAACTCTC GAGGAGTTAG CTCCCTCGCC CACGCCCGCC 
TTGGCCTTGG TGCCGCCGGC TCCCTCATCG GTCAAGAGCT CCATCTCCAA GTCGGTGAGT 
GTGGTCAATG TGACGGCGGC CAGAAAGTTT CAGCAGGAGC ATCAGAAGCA GCGTGAACGT 
GACCGTGAGC AGCTTAAGGA GCGCACCAAC TCCACGCAGG GCGTGATCCG GCAACTGAGC 
AGCTGCCTAA GCGAGGCGGA AACGGCATCC TGTATCCTAT CACCAGCCAG TAGCTTGAGT 
GCCAGCGAAG CACCGGACAC GCCCGATCCG CACAGCAACA CATCACCGCC ACCGTCGCTC 
CACACACGTC CCAGTGTCCT GCATCGAACC CTGACCAGCA CGCTGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Met ( NM_078571.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511126.2 Drosophila melanogaster Methoprene-tolerant (Met), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01301
Clone ID Related Accession (Same CDS sequence)	NM_001298203.1 , NM_078571.4
Accession Version	NM_078571.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Methoprene-tolerant, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078571.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAC CAGAGACGGG CAACACGGGC TCCACAGGAT CCGCTGGCTC GACAGGATCG 
GGATCGGGAT CGGGATCGGG AAGTGGGAGC TCCTCAGATC CAGCGAATGG ACGGGAGGCC 
CGTAACCTTG CCGAAAAACA GCGACGGGAT AAGCTTAATG CCAGCATCCA GGAGCTGGCC 
ACCATGGTAC CACATGCAGC CGAATCCTCC CGTCGCCTGG ACAAAACCGC CGTCCTTAGA 
TTCGCCACCC ATGGCCTGAG ACTTCAGTAT GTCTTTGGCA AGTCCGCTTC CAGACGTCGC 
AAGAAAACCG GCCTCAAGGG AACGGGTATG TCTGCCTCAC CTGTCGGAGA TCTACCCAAT 
CCCAGTCTGC ATCTAACGGA CACTCTAATG CAACTGCTGG ACTGCTGCTT CCTCACCCTA 
ACCTGCAGTG GCCAAATCGT TTTGGTATCC ACCAGCGTGG AGCAGCTATT GGGTCACTGT 
CAGTCCGATT TGTATGGCCA GAATCTACTG CAGATCACGC ATCCCGATGA TCAGGATCTG 
TTAAGACAGC AGCTAATACC CAGGGATATA GAGACCCTGT TCTATCAGCA TCAGCACCAC 
CAGCAGCAGG GGCACAATCC CCAGCAGCAC TCCACTTCCA CGTCGGCCTC AACTTCGGGC 
AGTGATCTGG AGGAGGAGGA AATGGAGACG GAGGAACACC GTCTGGGTCG GCAGCAGGGA 
GAGGCGGACG ATGACGAGGA TCACCCGTAC AACCGACGAA CACCCAGCCC GCGGAGAATG 
GCCCATTTGG CGACCATTGA TGACCGACTA CGCATGGATC GGCGCTGCTT TACCGTCCGC 
TTGGCTAGGG CTTCCACGCG AGCGGAGGCC ACGCGTCATT ACGAGCGGGT TAAGATCGAT 
GGCTGCTTTC GTCGCAGTGA CTCCTCCTTA ACCGGAGGTG CCGCTGCCAA CTATCCGATT 
GTCTCCCAGC TGATACGACG CTCGAGAAAC AACAATATGC TGGCTGCTGC TGCAGCAGTG 
GCAGCAGAAG CGGCGACGGT GCCGCCCCAG CACGATGCCA TTGCCCAGGC GGCGCTGCAC 
GGGATTAGCG GCAATGATAT TGTCCTGGTG GCCATGGCCA GGGTGCTGCG AGAGGAACGG 
CCGCCTGAGG AGACGGAGGG TACAGTGGGC TTGACCATTT ACAGACAGCC AGAACCCTAT 
CAGTTGGAGT ACCATACGAG GCATCTAATC GACGGCAGCA TCATCGACTG TGATCAAAGG 
ATTGGTCTGG TGGCGGGATA TATGAAGGAT GAGGTGCGCA ACCTTAGTCC CTTCTGTTTC 
ATGCACCTGG ACGACGTTCG CTGGGTGATT GTGGCCCTTC GACAAATGTA CGATTGCAAC 
AGTGACTACG GCGAGAGCTG CTACCGTCTG CTGTCCCGCA ACGGGCGCTT CATTTACCTG 
CACACCAAGG GATTTCTGGA GGTCGACCGT GGCAGTAATA AGGTGCATTC CTTTCTGTGC 
GTCAACACGC TGCTCGATGA GGAGGCGGGC CGGCAAAAGG TGCAGGAGAT GAAGGAGAAA 
TTCTCGACAA TCATCAAGGC GGAGATGCCC ACGCAGAGCA GCAGTCCCGA TTTGCCCGCC 
TCGCAGGCAC CGCAGCAACT TGAGAGAATT GTCCTCTATC TAATAGAGAA CCTACAGAAG 
AGTGTGGATT CAGCAGAGAC GGTTGGCGGC CAGGGCATGG AAAGCCTAAT GGACGATGGC 
TACAGTTCGC CAGCAAATAC CTTAACTCTC GAGGAGTTAG CTCCCTCGCC CACGCCCGCC 
TTGGCCTTGG TGCCGCCGGC TCCCTCATCG GTCAAGAGCT CCATCTCCAA GTCGGTGAGT 
GTGGTCAATG TGACGGCGGC CAGAAAGTTT CAGCAGGAGC ATCAGAAGCA GCGTGAACGT 
GACCGTGAGC AGCTTAAGGA GCGCACCAAC TCCACGCAGG GCGTGATCCG GCAACTGAGC 
AGCTGCCTAA GCGAGGCGGA AACGGCATCC TGTATCCTAT CACCAGCCAG TAGCTTGAGT 
GCCAGCGAAG CACCGGACAC GCCCGATCCG CACAGCAACA CATCACCGCC ACCGTCGCTC 
CACACACGTC CCAGTGTCCT GCATCGAACC CTGACCAGCA CGCTGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511126.2
CDS	745..2895
Translation	MAAPETGNTGSTGSAGSTGSGSGSGSGSGSSSDPANGREARNLAEKQRRDKLNASIQELATMVPHAAESSRRLDKTAVLRFATHGLRLQYVFGKSASRRRKKTGLKGTGMSASPVGDLPNPSLHLTDTLMQLLDCCFLTLTCSGQIVLVSTSVEQLLGHCQSDLYGQNLLQITHPDDQDLLRQQLIPRDIETLFYQHQHHQQQGHNPQQHSTSTSASTSGSDLEEEEMETEEHRLGRQQGEADDDEDHPYNRRTPSPRRMAHLATIDDRLRMDRRCFTVRLARASTRAEATRHYERVKIDGCFRRSDSSLTGGAAANYPIVSQLIRRSRNNNMLAAAAAVAAEAATVPPQHDAIAQAALHGISGNDIVLVAMARVLREERPPEETEGTVGLTIYRQPEPYQLEYHTRHLIDGSIIDCDQRIGLVAGYMKDEVRNLSPFCFMHLDDVRWVIVALRQMYDCNSDYGESCYRLLSRNGRFIYLHTKGFLEVDRGSNKVHSFLCVNTLLDEEAGRQKVQEMKEKFSTIIKAEMPTQSSSPDLPASQAPQQLERIVLYLIENLQKSVDSAETVGGQGMESLMDDGYSSPANTLTLEELAPSPTPALALVPPAPSSVKSSISKSVSVVNVTAARKFQQEHQKQRERDREQLKERTNSTQGVIRQLSSCLSEAETASCILSPASSLSASEAPDTPDPHSNTSPPPSLHTRPSVLHRTLTSTLR

Target ORF information:

RefSeq Version	NM_078571.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Methoprene-tolerant (Met), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078571.4

ORF Insert Sequence:

ATGGCAGCAC CAGAGACGGG CAACACGGGC TCCACAGGAT CCGCTGGCTC GACAGGATCG 
GGATCGGGAT CGGGATCGGG AAGTGGGAGC TCCTCAGATC CAGCGAATGG ACGGGAGGCC 
CGTAACCTTG CCGAAAAACA GCGACGGGAT AAGCTTAATG CCAGCATCCA GGAGCTGGCC 
ACCATGGTAC CACATGCAGC CGAATCCTCC CGTCGCCTGG ACAAAACCGC CGTCCTTAGA 
TTCGCCACCC ATGGCCTGAG ACTTCAGTAT GTCTTTGGCA AGTCCGCTTC CAGACGTCGC 
AAGAAAACCG GCCTCAAGGG AACGGGTATG TCTGCCTCAC CTGTCGGAGA TCTACCCAAT 
CCCAGTCTGC ATCTAACGGA CACTCTAATG CAACTGCTGG ACTGCTGCTT CCTCACCCTA 
ACCTGCAGTG GCCAAATCGT TTTGGTATCC ACCAGCGTGG AGCAGCTATT GGGTCACTGT 
CAGTCCGATT TGTATGGCCA GAATCTACTG CAGATCACGC ATCCCGATGA TCAGGATCTG 
TTAAGACAGC AGCTAATACC CAGGGATATA GAGACCCTGT TCTATCAGCA TCAGCACCAC 
CAGCAGCAGG GGCACAATCC CCAGCAGCAC TCCACTTCCA CGTCGGCCTC AACTTCGGGC 
AGTGATCTGG AGGAGGAGGA AATGGAGACG GAGGAACACC GTCTGGGTCG GCAGCAGGGA 
GAGGCGGACG ATGACGAGGA TCACCCGTAC AACCGACGAA CACCCAGCCC GCGGAGAATG 
GCCCATTTGG CGACCATTGA TGACCGACTA CGCATGGATC GGCGCTGCTT TACCGTCCGC 
TTGGCTAGGG CTTCCACGCG AGCGGAGGCC ACGCGTCATT ACGAGCGGGT TAAGATCGAT 
GGCTGCTTTC GTCGCAGTGA CTCCTCCTTA ACCGGAGGTG CCGCTGCCAA CTATCCGATT 
GTCTCCCAGC TGATACGACG CTCGAGAAAC AACAATATGC TGGCTGCTGC TGCAGCAGTG 
GCAGCAGAAG CGGCGACGGT GCCGCCCCAG CACGATGCCA TTGCCCAGGC GGCGCTGCAC 
GGGATTAGCG GCAATGATAT TGTCCTGGTG GCCATGGCCA GGGTGCTGCG AGAGGAACGG 
CCGCCTGAGG AGACGGAGGG TACAGTGGGC TTGACCATTT ACAGACAGCC AGAACCCTAT 
CAGTTGGAGT ACCATACGAG GCATCTAATC GACGGCAGCA TCATCGACTG TGATCAAAGG 
ATTGGTCTGG TGGCGGGATA TATGAAGGAT GAGGTGCGCA ACCTTAGTCC CTTCTGTTTC 
ATGCACCTGG ACGACGTTCG CTGGGTGATT GTGGCCCTTC GACAAATGTA CGATTGCAAC 
AGTGACTACG GCGAGAGCTG CTACCGTCTG CTGTCCCGCA ACGGGCGCTT CATTTACCTG 
CACACCAAGG GATTTCTGGA GGTCGACCGT GGCAGTAATA AGGTGCATTC CTTTCTGTGC 
GTCAACACGC TGCTCGATGA GGAGGCGGGC CGGCAAAAGG TGCAGGAGAT GAAGGAGAAA 
TTCTCGACAA TCATCAAGGC GGAGATGCCC ACGCAGAGCA GCAGTCCCGA TTTGCCCGCC 
TCGCAGGCAC CGCAGCAACT TGAGAGAATT GTCCTCTATC TAATAGAGAA CCTACAGAAG 
AGTGTGGATT CAGCAGAGAC GGTTGGCGGC CAGGGCATGG AAAGCCTAAT GGACGATGGC 
TACAGTTCGC CAGCAAATAC CTTAACTCTC GAGGAGTTAG CTCCCTCGCC CACGCCCGCC 
TTGGCCTTGG TGCCGCCGGC TCCCTCATCG GTCAAGAGCT CCATCTCCAA GTCGGTGAGT 
GTGGTCAATG TGACGGCGGC CAGAAAGTTT CAGCAGGAGC ATCAGAAGCA GCGTGAACGT 
GACCGTGAGC AGCTTAAGGA GCGCACCAAC TCCACGCAGG GCGTGATCCG GCAACTGAGC 
AGCTGCCTAA GCGAGGCGGA AACGGCATCC TGTATCCTAT CACCAGCCAG TAGCTTGAGT 
GCCAGCGAAG CACCGGACAC GCCCGATCCG CACAGCAACA CATCACCGCC ACCGTCGCTC 
CACACACGTC CCAGTGTCCT GCATCGAACC CTGACCAGCA CGCTGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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Met cDNA ORF clone, Drosophila melanogaster(fruit fly)

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ORF Insert Sequence:

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ORF Insert Sequence:

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