dsh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dsh
Entrez Gene ID	32078
Full Name	dishevelled
Synonyms	CG1720,CG18361,DSH,Dm Dsh,Dmel\CG18361,Dsh,Dvl,l(1)10Bd,l(1)G0267,l(1)G17,l(1)dsh
General protein information	Preferred Names dishevelled Names CG18361 gene product from transcript CG18361-RA CG18361-PA disheveled disshevelled dsh-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10B4-10B5\|1-34.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078563.3	NP_511118.2	dishevelled

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	dvl3	NP_001116929.1
Mus musculus (house mouse)	Dvl3	NP_031915.2
Homo sapiens (human)	DVL3	NP_004414.3
Pan troglodytes (chimpanzee)	DVL3	XP_516908.3
Bos taurus (cattle)	DVL3	XP_002684923.1
Gallus gallus (chicken)	DVL3	XP_004943453.1
Danio rerio (zebrafish)	LOC100149082	XP_001920242.3
Drosophila melanogaster (fruit fly)	dsh	NP_511118.2
Canis lupus familiaris (dog)	DVL3	XP_535822.2
Rattus norvegicus (Norway rat)	Dvl3	NP_001100551.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004448	XP_313739.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

APC/C(Fzr/Cdh1) negatively regulates Nek2, which negatively regulates Dsh, to ensure its proper stoichiometric requirement and localization during planar cell polarity establishment.
Title: APC/C

Exon junction complex (EJC) activity is indispensable for Wg signaling by maintaining an appropriate level of Dsh protein for Wg ligand reception in Drosophila. Genetic and biochemical experiments demonstrate that Dlg1 protein acts independently from its role in cell polarity to protect Dsh protein from lysosomal degradation.
Title: The exon junction complex regulates the splicing of cell polarity gene dlg1 to control Wingless signaling in development.

Klp64D forms a complex with Dsh and shows functional interaction.
Title: Kinesin-II recruits Armadillo and Dishevelled for Wingless signaling in Drosophila.

Mutations that compromise a direct interaction between Dishevelled and Notch reduce the efficacy of repression.
Title: Planar cell polarity controls directional Notch signaling in the Drosophila leg.

co-expression experiments reveal that APC displaces Dishevelled from Axin assemblies, promoting degradasome over signalosome formation in the absence of Wnts
Title: The Adenomatous polyposis coli tumour suppressor is essential for Axin complex assembly and function and opposes Axin's interaction with Dishevelled.

The following dsh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dsh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03496	NM_078563.3 Latest version!	Drosophila melanogaster dishevelled (dsh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dsh ( NM_078563.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511118.2 Drosophila melanogaster dishevelled (dsh), mRNA.

Price & Availability↑

CloneID	OFa03496
Clone ID Related Accession (Same CDS sequence)	NM_078563.3
Accession Version	NM_078563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dishevelled
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_078563.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGCGG ACAGGGGCGG CGGGCAGGAG ACGAAGGTGA TATACCACAT CGACGATGAG 
ACGACGCCGT ATCTGGTGAA GATCCCCATT CCATCCGCCC AGGTGACGCT GCGCGATTTC 
AAGCTGGTGC TGAACAAGCA GAACAACAAC TACAAGTACT TCTTCAAGTC AATGGACGCC 
GATTTCGGTG TGGTCAAAGA GGAAATAGCC GACGACTCCA CCATACTGCC CTGCTTCAAT 
GGGCGAGTGG TGTCCTGGCT GGTCTCCGCC GATGGTACCA ATCAGTCAGA TAACTGCTCC 
GAGCTGCCAA CCAGTGAGTG TGAATTGGGC ATGGGTCTGA CCAACAGGAA GCTACAGCAG 
CAACAGCAGC AGCATCAGCA GCAGCAACAA CAACAGCAGC AGCAGCACCA GCAACAGCAA 
CAACAACAGC AGCAGCAGGT GCAGCCTGTC CAGCTGGCGC AGCAGCAACA GCAGCAGGTG 
CTCCATCACC AGAAGATGAT GGGCAATCCG CTGCTGCAGC CGCCACCGCT CACATATCAA 
TCGGCATCCG TGCTCTCCAG CGATCTGGAC TCGACCAGTC TCTTTGGCAC CGAATCCGAA 
TTGACGCTGG ACCGCGACAT GACCGACTAT AGCAGCGTTC AGCGATTGCA GGTGCGCAAG 
AAGCCGCAGA GGCGCAAAAA ACGGGCGCCC AGCATGTCGC GCACCTCCTC GTACAGCTCC 
ATAACCGACT CGACCATGTC CCTAAATATC ATTACCGTCT CCATCAACAT GGAGGCGGTC 
AACTTTCTGG GCATATCCAT TGTCGGGCAA TCGAATCGCG GCGGCGACGG CGGCATCTAC 
GTGGGCAGCA TCATGAAAGG CGGCGCAGTG GCACTCGATG GACGCATCGA ACCGGGCGAC 
ATGATCCTCC AAGTGAACGA TGTCAACTTC GAGAACATGA CCAACGACGA GGCGGTACGT 
GTGCTGAGAG AAGTCGTTCA GAAGCCGGGA CCCATCAAGC TGGTGGTGGC CAAGTGCTGG 
GACCCAAATC CCAAGGGCTA CTTCACCATT CCGCGCACGG AGCCGGTGCG ACCCATCGAT 
CCCGGTGCTT GGGTGGCGCA CACTCAGGCC CTCACATCGC ACGACAGTAT TATTGCCGAC 
ATTGCGGAGC CGATTAAGGA GCGTCTGGAC CAGAATAACC TCGAGGAGAT CGTTAAGGCG 
ATGACGAAGC CGGACAGCGG CCTGGAGATC AGGGATCGCA TGTGGCTAAA GATAACGATT 
CCCAATGCAT TTATCGGCGC CGATGCGGTC AATTGGGTGC TGGAGAATGT GGAGGATGTT 
CAGGATAGGC GGGAGGCGCG AAGGATCGTC TCGGCGATGC TGCGCAGCAA TTACATCAAG 
CATACGGTCA ATAAGTTGAC CTTCTCGGAG CAGTGCTACT ACGTGGTGAA CGAGGAGCGC 
AATCCCAATC TGTTGGGCCG AGGACATCTG CATCCGCACC AGCTGCCGCA CGGGCATGGC 
GGCCATGCGC TTAGCCATGC GGACACCGAG AGCATCACCA GCGACATCGG GCCGCTACCG 
AACCCGCCCA TTTATATGCC ATATTCGGCC ACGTACAATC CAAGTCACGG CTATCAGCCG 
ATCCAGTACG GCATCGCCGA GCGACACATC TCGTCGGGAT CGAGTAGCAG CGATGTGCTG 
ACCAGCAAGG ATATCTCGGC GTCGCAGAGC GACATCACCT CGGTGATCCA TCAGGCCAAC 
CAGCTGACCA TCGCCGCTCA TGGCTCTAAC AAATCATCCG GCTCCTCCAA TCGGGGCGGC 
GGCGGAGGCG GTGGCGGTGG CGGCAACAAT ACCAACGATC AGGACGTATC CGTATTTAAT 
TACGTATTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511118.2
CDS	474..2345
Translation	MDADRGGGQETKVIYHIDDETTPYLVKIPIPSAQVTLRDFKLVLNKQNNNYKYFFKSMDADFGVVKEEIADDSTILPCFNGRVVSWLVSADGTNQSDNCSELPTSECELGMGLTNRKLQQQQQQHQQQQQQQQQQHQQQQQQQQQQVQPVQLAQQQQQQVLHHQKMMGNPLLQPPPLTYQSASVLSSDLDSTSLFGTESELTLDRDMTDYSSVQRLQVRKKPQRRKKRAPSMSRTSSYSSITDSTMSLNIITVSINMEAVNFLGISIVGQSNRGGDGGIYVGSIMKGGAVALDGRIEPGDMILQVNDVNFENMTNDEAVRVLREVVQKPGPIKLVVAKCWDPNPKGYFTIPRTEPVRPIDPGAWVAHTQALTSHDSIIADIAEPIKERLDQNNLEEIVKAMTKPDSGLEIRDRMWLKITIPNAFIGADAVNWVLENVEDVQDRREARRIVSAMLRSNYIKHTVNKLTFSEQCYYVVNEERNPNLLGRGHLHPHQLPHGHGGHALSHADTESITSDIGPLPNPPIYMPYSATYNPSHGYQPIQYGIAERHISSGSSSSDVLTSKDISASQSDITSVIHQANQLTIAAHGSNKSSGSSNRGGGGGGGGGGNNTNDQDVSVFNYVL

Target ORF information:

RefSeq Version	NM_078563.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dishevelled (dsh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078563.3

ORF Insert Sequence:

ATGGACGCGG ACAGGGGCGG CGGGCAGGAG ACGAAGGTGA TATACCACAT CGACGATGAG 
ACGACGCCGT ATCTGGTGAA GATCCCCATT CCATCCGCCC AGGTGACGCT GCGCGATTTC 
AAGCTGGTGC TGAACAAGCA GAACAACAAC TACAAGTACT TCTTCAAGTC AATGGACGCC 
GATTTCGGTG TGGTCAAAGA GGAAATAGCC GACGACTCCA CCATACTGCC CTGCTTCAAT 
GGGCGAGTGG TGTCCTGGCT GGTCTCCGCC GATGGTACCA ATCAGTCAGA TAACTGCTCC 
GAGCTGCCAA CCAGTGAGTG TGAATTGGGC ATGGGTCTGA CCAACAGGAA GCTACAGCAG 
CAACAGCAGC AGCATCAGCA GCAGCAACAA CAACAGCAGC AGCAGCACCA GCAACAGCAA 
CAACAACAGC AGCAGCAGGT GCAGCCTGTC CAGCTGGCGC AGCAGCAACA GCAGCAGGTG 
CTCCATCACC AGAAGATGAT GGGCAATCCG CTGCTGCAGC CGCCACCGCT CACATATCAA 
TCGGCATCCG TGCTCTCCAG CGATCTGGAC TCGACCAGTC TCTTTGGCAC CGAATCCGAA 
TTGACGCTGG ACCGCGACAT GACCGACTAT AGCAGCGTTC AGCGATTGCA GGTGCGCAAG 
AAGCCGCAGA GGCGCAAAAA ACGGGCGCCC AGCATGTCGC GCACCTCCTC GTACAGCTCC 
ATAACCGACT CGACCATGTC CCTAAATATC ATTACCGTCT CCATCAACAT GGAGGCGGTC 
AACTTTCTGG GCATATCCAT TGTCGGGCAA TCGAATCGCG GCGGCGACGG CGGCATCTAC 
GTGGGCAGCA TCATGAAAGG CGGCGCAGTG GCACTCGATG GACGCATCGA ACCGGGCGAC 
ATGATCCTCC AAGTGAACGA TGTCAACTTC GAGAACATGA CCAACGACGA GGCGGTACGT 
GTGCTGAGAG AAGTCGTTCA GAAGCCGGGA CCCATCAAGC TGGTGGTGGC CAAGTGCTGG 
GACCCAAATC CCAAGGGCTA CTTCACCATT CCGCGCACGG AGCCGGTGCG ACCCATCGAT 
CCCGGTGCTT GGGTGGCGCA CACTCAGGCC CTCACATCGC ACGACAGTAT TATTGCCGAC 
ATTGCGGAGC CGATTAAGGA GCGTCTGGAC CAGAATAACC TCGAGGAGAT CGTTAAGGCG 
ATGACGAAGC CGGACAGCGG CCTGGAGATC AGGGATCGCA TGTGGCTAAA GATAACGATT 
CCCAATGCAT TTATCGGCGC CGATGCGGTC AATTGGGTGC TGGAGAATGT GGAGGATGTT 
CAGGATAGGC GGGAGGCGCG AAGGATCGTC TCGGCGATGC TGCGCAGCAA TTACATCAAG 
CATACGGTCA ATAAGTTGAC CTTCTCGGAG CAGTGCTACT ACGTGGTGAA CGAGGAGCGC 
AATCCCAATC TGTTGGGCCG AGGACATCTG CATCCGCACC AGCTGCCGCA CGGGCATGGC 
GGCCATGCGC TTAGCCATGC GGACACCGAG AGCATCACCA GCGACATCGG GCCGCTACCG 
AACCCGCCCA TTTATATGCC ATATTCGGCC ACGTACAATC CAAGTCACGG CTATCAGCCG 
ATCCAGTACG GCATCGCCGA GCGACACATC TCGTCGGGAT CGAGTAGCAG CGATGTGCTG 
ACCAGCAAGG ATATCTCGGC GTCGCAGAGC GACATCACCT CGGTGATCCA TCAGGCCAAC 
CAGCTGACCA TCGCCGCTCA TGGCTCTAAC AAATCATCCG GCTCCTCCAA TCGGGGCGGC 
GGCGGAGGCG GTGGCGGTGG CGGCAACAAT ACCAACGATC AGGACGTATC CGTATTTAAT 
TACGTATTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dsh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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