nocte cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nocte
Entrez Gene ID	31976
Full Name	no circadian temperature entrainment
Synonyms	CG17255,Dmel\CG17255
General protein information	Preferred Names no circadian temperature entrainment Names CG17255 gene product from transcript CG17255-RD CG17255-PA CG17255-PB CG17255-PC CG17255-PD nocte-PA nocte-PB nocte-PC nocte-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 9C5-9D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258685.2	NP_001245614.1	no circadian temperature entrainment, isoform C
NM_167231.5	NP_727425.3	no circadian temperature entrainment, isoform B
NM_001272480.1	NP_001259409.1	no circadian temperature entrainment, isoform D
NM_132403.5	NP_572631.3	no circadian temperature entrainment, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nocte gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nocte cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01233	NM_167231.5 Latest version!	Drosophila melanogaster no circadian temperature entrainment (nocte), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa01233	NM_132403.5 Latest version!	Drosophila melanogaster no circadian temperature entrainment (nocte), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa01233	NM_001258685.2 Latest version!	Drosophila melanogaster no circadian temperature entrainment (nocte), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa03412	NM_001272480.1 Latest version!	Drosophila melanogaster no circadian temperature entrainment (nocte), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nocte ( NM_001258685.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245614.1 Drosophila melanogaster no circadian temperature entrainment (nocte), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01233
Clone ID Related Accession (Same CDS sequence)	NM_001258685.2 , NM_167231.5 , NM_132403.5
Accession Version	NM_001258685.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	no circadian temperature entrainment, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258685.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTACAC TGGGGGGAAG TAGGGGAGAG CGAAATGCCA AGCCCAAATT CACAGCGTTA 
GATATAAATC GCATGTACAA GAATAGCCGC GGCGAGTCAA GTGAACCATC TGCACAAAAG 
AATCAAGTGC CACGTAAACA TGGAATGCAA ATCTTGGGCA AAGTGCCGTC CGCTAGGCGA 
CCACCAGCCA ATTTGCCATC CCTGAAGGCC GAGACCCACA CCAGCAGCAA CAACAATCTC 
TTAGTCTCAG TCGAAGGAGC CACATCTGGT GGGGCGGGCG CAGGCGGAGC AGCGGGATCT 
GGCGCGGCAG GAACCAACAC TAGCCACAAC AACACCACTG CCCACAGCGG AAGTTCAGGA 
ACGAGCGGCG GAGCGGGATC AGGCGGCGGA GGAACTGCGT CTTCATCTGC TGGTGGCCAT 
CTATCCCAGC AGCTGACTCA CAGCCAGCAT CAGCACAATA ATAACGTCGC TCTCGGCCCT 
AACGCCGGAA AGTTTGTCAA CAGCAACAAC GGCAGCAGCA ACAACAACGC CGGCAGCTCA 
GGCAGCGGCA ACAAGAACTT CAAATTAATC AACAACTCCG TGGGCAGTTC CGGCGGAGGC 
GGACAAGGAG GGTCGGGCGG CGGCGGATCG TCAGGAGTAG GAGGTGGATC AGGGTCAGGA 
TCGGGCGGAC ACTCAGTCAA CTCACCCAAG ACATGGTCTG CAATTACCAC AGGACACGAG 
CGGGGCTCGG GCGGTCAGAA CTATGGACGC CAGCAACAGC AGGTGGTCCC CCATTATCAG 
AGTCCACAGT TCCAGTACGA ATTTCCCACG CTGGACGGCA CAGTTGGCAG CGGCGCCGGC 
GGAGGATCGG GAGCGGGTGT GGGTTCAGGA TCTGGAGCGG GCAAGAGCCA TTACCAGAAT 
CAGGTGCACC ATGCCGGCCA CCAAAACCAT CACCAGCATC AGCAGCACCA CCAGCAGCAT 
CAACAGCACC ACCACCAGCA GCAACAGCAC CATCGGGACT ACCACCATGG CCACGGGCGC 
CAGTATGGAC ACCACTCGCA TCGCGACTAC AGAGATGGCA GCGACGACGT CGGCGGCGGG 
TCCGCGGATC TGGGCGAGCT CAGCCTTCGG CCACAGAACG ACACGGCCGC CTGGTTGCAG 
CAGCAGGAGA AGGCGGCCAA GGTGGCAGCA GCTGCGGCAG CGGACCAGGG CCTCAACCTA 
CAGGGCCCGG GCCACCATCA GATGGGCGGT AATGGCGGAG CAGGAGGAGG GTCGGGCGGA 
GCTGTGCCCC TGCCCATACT ATCGCTGATG CCCTCGTTCA TGCGTAGCGG AGCTCCGATG 
CCCGCCAGTG GAGCGACAGC GGCCAGCATC TTGGCCGGCG GATCGTTGGA CACTTCTGCG 
TTGCCCAACG CTCCCTACCA GAACGGAATC CTGGGTGCTC GGTCTGCCTC GCCAGCTGTG 
GTGGGCAGTG GAAGCTTCAG GGCGGCCACT GCCATTCCCA AGCGACCACC GACCTCCTCG 
TCGCCCCCAC AATCTGGCCT AGGCATTGGA GGATCACCCA CTCCCCCACA ATCCCAGCAG 
CAGGCCAACG GAGGCGCTGG CGGAGCCGGG CGCAAGGATA AGGACTACGT GGTGGAGCCG 
GAGGTGGCCC AAATGCAGCG TCCTATCATA CGCGAAGAGG ACTTGGAGCG CCTCAACGCC 
ATCGCCAAGG ACGATAGCTG GACCAAGCAG GACGACATTG ACTTCACGAA GAAACTAGCA 
TTCTCGGACG ATGAGTCCAC TCCGGAAGAG CACCAAGGCC AGGGCAAGCA GCACAGCTCC 
GCCCAACTGC AGGCGCAAGG ATCCTCCTCC TCAACCGATC AACGAAAGCT GTCCGCTTCC 
ACAACCAGTT GGCAGCGCGG CAAGGAGAGC ACCGATCGCG ACCAGCCATC GGAAGGCATT 
CAGGATCAGC AGCAGCAGCT TCAGCAGCAA CAGCAGCAGC AAGAGGTTCG CCAACAGAAT 
GGCCACCGCG GTAGCTCTGG CAACGTTGGA GCACCAGTGG CCGGTGGAAT TGCCTTGGAT 
GCCAGCGTGT ACGAGCGAGT AAAGCAGCGC AAGGAGGAGG AAGAGCGTCG CGAGATGGAA 
CGCAAGCAGG CGGCTGCTAA GAAGTTGCAA GAACTGGAAA TGAAGATGAA TAGCAAAAAG 
GCAGCGGCTG CGGCTCTGGC TGGAGCCGAG GGCGGCGTGT CCTCTGCCTC AGTCCCGTTG 
AGCAATGAGG TGCCACCCAC GTCCGCACCG CTAGGCAATG CCAGCGAAGA TGAGGGCGGA 
GGCCAAGGCG GCGGCGGAGG GTTGAATCGT CGACCCCGGG GAAGCATCTC TAGCTTGGGA 
AGCGGCGTAA CACCCACGGG AGGAGCTGGC GGCGGCGGCG GAGGAGTTTC GTCATCCTCG 
TCAGCAGGCG GCGACTATGG TCAAAAGAAC TTCCTTACCC ATTTCCAATC AAATTTACCA 
CCACGTTTCC AGCGCCAGCA ACAGCAACAA CAGCAGCAGC TGCCGCGCTT GGAGAAGAGC 
GCCTCGGCAA GCAGTGCATT CGATAGCAAT TCCCGCTACC TCCAGAAGGG AGGAGTAAGC 
GGAGGAGGCG GTGGTGTAAG TGGAGGAGCG CAAGCCAATG CTGGCCAGGG TCGCAGCATC 
TCCGGCGGTT ATGTGCAGCG TGGTGCAAGT GTCGGAGGAA GCAGCGGATA CGGACGAAAT 
CGTCACGACA GCAGCAGTGC TCGGGAGGAT CAGGATCTGG ATCAGCAGCG TTTCACCCGT 
GGCCAGGACT ATCGGCAGGC ACAAGCTCTG CCGCGCAGCA TATCTGAGAA CTCGCACCGT 
AAGACCAGCG TCTCATCGGC TGGCGACGAT GTGCTGGGTG GCTCGTGCTC CTCATGGGCG 
GAGCAAACGG ATGCCGAGCA GAAGGTGCAC CATCGGCACC GCGAGGAGAG CTTCTCCTCG 
TCGCACAGCC ATGACTCGGC AGTGCAAATA AAGATCCTGC AACGCCCGGC CCGACAGCCG 
AGCCTCAGTG AGGAGTCCTC GACCCAGCAG AAACAGCTGC CCGCCTCGCC GCAGCAGCAA 
AAGACGCTGA CGACAGATCC CATGCCTACC CAGATTCTGC GACGCAGCGT GGATATCGAT 
AAGTCCGGTG ATGAGCGCGC GGAGGACAAG TCTGCATTGG AATCTGATGA CAAGGCGAAG 
TCGGCTGGCA AGGTGGAGGA GGACAAGAGC GGCAATAAGC GGGTAAGTCC CAGGAGCGGA 
GCTGCTTGCG GAGGTCGAGG TGGCCGTGGT TATGCCGGCA GCTCTGGTGG ATCATCCGTC 
GGATCGGGCA GTAGCTACCG CGGCCAGAGA AATGCCAGCG ATTGGGGAAG CAGTCGTGGA 
AGCGGCGGCG CCGGCGGCAG GCGCTACTAC GGCAGCGGCG GTGAGCAATC GGAGCACTCC 
GAGGATGTGG ACGAGGAGTG TTACAGCAGT GGAGGAGGCG GAGCCGCTCG TCGCAGCCCA 
AAGGAATCGG GCAACGGTGC GCCCAATTCC GGTGCGTCGG CAAACAAGGC AGGATTCTCG 
CCACGCGGCG AACCATCTAG ACGTGGTCGA GGCGGCCTCT CTAGCGGAGG AGGCGCCTCT 
GCTTCTTGTT ACCGTCGACA GCCCGTGGGC ACTGGATCCG CTGGTGGATC TGGTTCGGGC 
GGATCGGGCC GCAGCTATGG CAGACTTGGC TACGAGGAAT ATGGCAAGCA ACAGCGAACT 
TCCGAGTCGG AGACGGACCT AGACAAGACC AAGCAGAACC AGCTGGCACT CAGCGCCGGC 
CTGCACAAAC CAAGCAAGGA TGACAAGGAT TTGCCTGCGC TTGGGGGCGA GGAGAAGGAC 
AAGACTGCCG CCCTGGCGCC TGCCCATAAC ACTGCCCTGC TAGCAAAGGA TGAATTACAG 
AAAGATGCAG CGGGTCGCCT GCCGTTGCCT CCCGGCCTCG CAGCACCTGG CTCTGCAGTG 
GGATCCGTTA CTGCCACATC CTCATCGGTC GTAGTTCCCG GTGCAGCGGA CAAGAAGAAG 
ATTGAATCCT GCGCCGTTGT CGGCGGTGAG AAGGTGACGG GTAGCAAGTT GCCCGCCATT 
GGGGAAAAGA CTTCGCTCGG CGCCGTTGGC TCGGGTCCAT CAACCAGTCT TCTACTGGAC 
GCCGGCGCAC CCGTCAACAC GATTATATTT GAGAACTCCA CGTACAAGCA GCAGGCAGCT 
GCCGCTTCCG TGGTAACCAA ACCCGGAACG CTCTCGGTGT CAAGCAGTGG CGCCACCATG 
ACCGTGGACA GCCTGTCCAG CGCACTCTCC CAAATGAGTT TCGGCGGCAA GGCGACGGCG 
GCGGGCTCTG GCGATGATTC GCAGGATATG AAACTAGGAT TTAGCTTCGG TGACGATCCC 
ACCACACCCC TCAAGGTTGT GGATAACATC GATAAAACGG CCAGTCAGCA GCAGCAACAG 
CAGCAGCAAC AACACTCTCA ACAGCAACAA CAACAGCAGC AGCAGCAAAA CAATTCGACG 
GCGGATCTTA ACATGAAGAT AGCCAGCGTA AAGAAGGTTT GGGAGTCAGC CACGCCCATG 
TCCGACGGCC CATCGCCAGC ACAGGTGCAG CAGCAACAGC AACAGCAGCC CCAACAGCAA 
CAACAGTCCC AGGTGCAGGT GGCGGCGCAG CAGCAACAAC AGCAGGTGGT GGGACCGCAG 
CAGGCAGGTG ACGACGGCAG TGGTCACATG AGCGCCGCCT CCTTTGTGGC AGCCGTGGCC 
GCCTCACAGC ATCAGCAGCA ACACCAGCAA ATGCCGCACT ACGCGGTGTC GGCGGTGCAC 
ATGCAGAGCC ACCACCACCA ACAGCAGCAG CAACAGCAGC AGGCAGCGGT GGCGGCAGCG 
CAGGCGCAGC ACCACCAGCA GCAACAGGCA CAGGCGCACC ACCAGCACCA TGCACTTTCC 
TCGCCGGGCC CGGTGCCCGG TAGTCATGGC CATGGCTACA GTCATGGATC GCCGTTCGAC 
GCTGGATCGT TGGAGCAACA GTTCCAGCAA GAGGATTATC CCAGTCCACA GCAGCAACAG 
CAGGCGCACC AGAAGACCAA GCAGTTGCAG CAGCAGCAGC ATCTGGGCAT GTCACCGCCG 
CCCAGCCAGC AGCAACAGCA GCAGCACTCG CAACAGCAGC AGCAAGCACA GCACCAGCAA 
CAACAGCAGC AGGCCCATCA GCAGCAGCAA CAGCAGCAGC AGCCTTCATT CTACCAGGCG 
TCACCGCAGT TCGGCGTTGG AGGCATTCCC ACTATCCCCA GTCCGCCGGC CGTGGTGTAC 
AACTCGTCGC AGATGCCGCC ACCGTCGCAG GGGGGTAATC TGTACGCTCC ATTCCATTCG 
CTCGATCATT CGAGCCGTTC GCCCGCCTAC TCGGCGGCGG CGGCCGCCCA CAGCTACCCG 
AACCCTTATG CGGCAGCAGC AGCGGCGGCG GCGGCAGCGG CCGCTGGCGG AGGTCCCTTC 
AATGTCAACG TGAACGCATA TGCGATGCAG ACTCCGCACG GCGGCAGCCT ACCGCCCACA 
GCGCCCACAC CGGACATGTA CTCGAACCTC TCTTCGCAGT TTCGATTGGG CGGTGGTCCG 
GGTCCCGGAC CCGGTCCCTT TGGCCAGCCC AGCTCGCAGC AGTTAAGCAA TCCGAGCAAT 
CATGCTGCGG TGGCTATAAT TTCGTCAAGC AGCAACTCCA TGATGTCGTC AGGTGCGGCC 
AAACCGCCGC AGAGCCAGCA GACAATCGGT GCCATTGGCT CCAAGTCGGC AGGTAGCGGT 
GGCGGTCCAG GACCAGGACC TGGTCCCTAT GCCACCACCC AGACGTATAT GAATCTTTAT 
GCGGCTCCAC CGCCGCAACA TCCAGGACAA GGTGGACCAC CGCCGGGTGC CCATCAGCTG 
CAGTCGAACA GTTTCTACTC GAATTCGGCG CCAGGCGGTC CGAGCGGACC ACAGTTCTAC 
GGTGGTCCGC CGCCGCAGGG CAATGGTGGA GCGCAGAGCT ATGGACTGGC CACCGCGGCC 
GGCATGTATG GCGGCCATCA GGGCGGACCG CCCGGCTCCA ATGGGCCGCC GGGACCGCCG 
CAGTCGCAGC ACACGATGGG CACGTTCAAC ACGCCGTTCA TGAACTCGCA GCTGCTGACG 
GCGGCCAGCA TTAATCAGTA CCGCGGCGGA CCAACGCCTG GAGCGGCTTA TCTCAAGGGT 
AACCAGGGCC AGACACACAT GCAAGACTCG ATGGGTCGTC AGCTGAAGAG TCCGCTGAGC 
GCTGACATGA GCCTCGGTCT GGCCAAGCAG GTGCAGTCGC AGCCCAGTCC ACCGCATCAC 
AAGAACTACG GCGCGTGGGA TCTGCAGAAC CAGGTGCTTC AGCAGCAGCA GCAGCAATCT 
CAGCAGCAAC AGCAGCAGCA ACAGCGCCAG GTCGGCGGCG GCAATGGTCC GAGCATGAAC 
GCCTTGGGCG GTCGCGGCAG TGGAGCGGTG GGCAGCGGCA GCGGCAACGG CGGAGGAGGA 
GGAGGAGGAG CTGGTGGGTC CCAAGTGGGC GGCAATGGCG GCGGTAACGG CGTTGGCAGC 
GTGGGCCAAA GTGGCGGCGG CGGCCAGGGA CGCTATCCCA CGCCCATTCA GCGGCCCAAC 
AACTATCCGC AGCATCCGCA ACAACAGCAG CAACAACAGC AGCAACAGCA ACAGCAGAGA 
GAGCAGGCGG CAGCTGCCGC CGTGGCGGCT CAGCGGGCAC AGAATATGCG ACAGGTGACC 
GGTGCCGGCG GAGGCGTGGC CGGAAGTGCA GGTGGCGGTG GTAGTCAAAC ACCAGTGGGA 
CCGGCCGGCG GCAATGGTGG CCCCGGTGGC GCAGGAGGAG GCGGTGGAGC TGGCGGTGCA 
GCAGGATCAG GACCCGGAAA GCCCTACTAT GCGAATAACG CTAGCGGAGC GGCCGTTGCG 
GCCAATCGAG GTGAGTGGCA TGCGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245614.1
CDS	337..7266
Translation	MSTLGGSRGERNAKPKFTALDINRMYKNSRGESSEPSAQKNQVPRKHGMQILGKVPSARRPPANLPSLKAETHTSSNNNLLVSVEGATSGGAGAGGAAGSGAAGTNTSHNNTTAHSGSSGTSGGAGSGGGGTASSSAGGHLSQQLTHSQHQHNNNVALGPNAGKFVNSNNGSSNNNAGSSGSGNKNFKLINNSVGSSGGGGQGGSGGGGSSGVGGGSGSGSGGHSVNSPKTWSAITTGHERGSGGQNYGRQQQQVVPHYQSPQFQYEFPTLDGTVGSGAGGGSGAGVGSGSGAGKSHYQNQVHHAGHQNHHQHQQHHQQHQQHHHQQQQHHRDYHHGHGRQYGHHSHRDYRDGSDDVGGGSADLGELSLRPQNDTAAWLQQQEKAAKVAAAAAADQGLNLQGPGHHQMGGNGGAGGGSGGAVPLPILSLMPSFMRSGAPMPASGATAASILAGGSLDTSALPNAPYQNGILGARSASPAVVGSGSFRAATAIPKRPPTSSSPPQSGLGIGGSPTPPQSQQQANGGAGGAGRKDKDYVVEPEVAQMQRPIIREEDLERLNAIAKDDSWTKQDDIDFTKKLAFSDDESTPEEHQGQGKQHSSAQLQAQGSSSSTDQRKLSASTTSWQRGKESTDRDQPSEGIQDQQQQLQQQQQQQEVRQQNGHRGSSGNVGAPVAGGIALDASVYERVKQRKEEEERREMERKQAAAKKLQELEMKMNSKKAAAAALAGAEGGVSSASVPLSNEVPPTSAPLGNASEDEGGGQGGGGGLNRRPRGSISSLGSGVTPTGGAGGGGGGVSSSSSAGGDYGQKNFLTHFQSNLPPRFQRQQQQQQQQLPRLEKSASASSAFDSNSRYLQKGGVSGGGGGVSGGAQANAGQGRSISGGYVQRGASVGGSSGYGRNRHDSSSAREDQDLDQQRFTRGQDYRQAQALPRSISENSHRKTSVSSAGDDVLGGSCSSWAEQTDAEQKVHHRHREESFSSSHSHDSAVQIKILQRPARQPSLSEESSTQQKQLPASPQQQKTLTTDPMPTQILRRSVDIDKSGDERAEDKSALESDDKAKSAGKVEEDKSGNKRVSPRSGAACGGRGGRGYAGSSGGSSVGSGSSYRGQRNASDWGSSRGSGGAGGRRYYGSGGEQSEHSEDVDEECYSSGGGGAARRSPKESGNGAPNSGASANKAGFSPRGEPSRRGRGGLSSGGGASASCYRRQPVGTGSAGGSGSGGSGRSYGRLGYEEYGKQQRTSESETDLDKTKQNQLALSAGLHKPSKDDKDLPALGGEEKDKTAALAPAHNTALLAKDELQKDAAGRLPLPPGLAAPGSAVGSVTATSSSVVVPGAADKKKIESCAVVGGEKVTGSKLPAIGEKTSLGAVGSGPSTSLLLDAGAPVNTIIFENSTYKQQAAAASVVTKPGTLSVSSSGATMTVDSLSSALSQMSFGGKATAAGSGDDSQDMKLGFSFGDDPTTPLKVVDNIDKTASQQQQQQQQQHSQQQQQQQQQQNNSTADLNMKIASVKKVWESATPMSDGPSPAQVQQQQQQQPQQQQQSQVQVAAQQQQQQVVGPQQAGDDGSGHMSAASFVAAVAASQHQQQHQQMPHYAVSAVHMQSHHHQQQQQQQQAAVAAAQAQHHQQQQAQAHHQHHALSSPGPVPGSHGHGYSHGSPFDAGSLEQQFQQEDYPSPQQQQQAHQKTKQLQQQQHLGMSPPPSQQQQQQHSQQQQQAQHQQQQQQAHQQQQQQQQPSFYQASPQFGVGGIPTIPSPPAVVYNSSQMPPPSQGGNLYAPFHSLDHSSRSPAYSAAAAAHSYPNPYAAAAAAAAAAAAGGGPFNVNVNAYAMQTPHGGSLPPTAPTPDMYSNLSSQFRLGGGPGPGPGPFGQPSSQQLSNPSNHAAVAIISSSSNSMMSSGAAKPPQSQQTIGAIGSKSAGSGGGPGPGPGPYATTQTYMNLYAAPPPQHPGQGGPPPGAHQLQSNSFYSNSAPGGPSGPQFYGGPPPQGNGGAQSYGLATAAGMYGGHQGGPPGSNGPPGPPQSQHTMGTFNTPFMNSQLLTAASINQYRGGPTPGAAYLKGNQGQTHMQDSMGRQLKSPLSADMSLGLAKQVQSQPSPPHHKNYGAWDLQNQVLQQQQQQSQQQQQQQQRQVGGGNGPSMNALGGRGSGAVGSGSGNGGGGGGGAGGSQVGGNGGGNGVGSVGQSGGGGQGRYPTPIQRPNNYPQHPQQQQQQQQQQQQQREQAAAAAVAAQRAQNMRQVTGAGGGVAGSAGGGGSQTPVGPAGGNGGPGGAGGGGGAGGAAGSGPGKPYYANNASGAAVAANRGEWHAN

Target ORF information:

RefSeq Version	NM_001258685.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster no circadian temperature entrainment (nocte), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258685.2

ORF Insert Sequence:

ATGAGTACAC TGGGGGGAAG TAGGGGAGAG CGAAATGCCA AGCCCAAATT CACAGCGTTA 
GATATAAATC GCATGTACAA GAATAGCCGC GGCGAGTCAA GTGAACCATC TGCACAAAAG 
AATCAAGTGC CACGTAAACA TGGAATGCAA ATCTTGGGCA AAGTGCCGTC CGCTAGGCGA 
CCACCAGCCA ATTTGCCATC CCTGAAGGCC GAGACCCACA CCAGCAGCAA CAACAATCTC 
TTAGTCTCAG TCGAAGGAGC CACATCTGGT GGGGCGGGCG CAGGCGGAGC AGCGGGATCT 
GGCGCGGCAG GAACCAACAC TAGCCACAAC AACACCACTG CCCACAGCGG AAGTTCAGGA 
ACGAGCGGCG GAGCGGGATC AGGCGGCGGA GGAACTGCGT CTTCATCTGC TGGTGGCCAT 
CTATCCCAGC AGCTGACTCA CAGCCAGCAT CAGCACAATA ATAACGTCGC TCTCGGCCCT 
AACGCCGGAA AGTTTGTCAA CAGCAACAAC GGCAGCAGCA ACAACAACGC CGGCAGCTCA 
GGCAGCGGCA ACAAGAACTT CAAATTAATC AACAACTCCG TGGGCAGTTC CGGCGGAGGC 
GGACAAGGAG GGTCGGGCGG CGGCGGATCG TCAGGAGTAG GAGGTGGATC AGGGTCAGGA 
TCGGGCGGAC ACTCAGTCAA CTCACCCAAG ACATGGTCTG CAATTACCAC AGGACACGAG 
CGGGGCTCGG GCGGTCAGAA CTATGGACGC CAGCAACAGC AGGTGGTCCC CCATTATCAG 
AGTCCACAGT TCCAGTACGA ATTTCCCACG CTGGACGGCA CAGTTGGCAG CGGCGCCGGC 
GGAGGATCGG GAGCGGGTGT GGGTTCAGGA TCTGGAGCGG GCAAGAGCCA TTACCAGAAT 
CAGGTGCACC ATGCCGGCCA CCAAAACCAT CACCAGCATC AGCAGCACCA CCAGCAGCAT 
CAACAGCACC ACCACCAGCA GCAACAGCAC CATCGGGACT ACCACCATGG CCACGGGCGC 
CAGTATGGAC ACCACTCGCA TCGCGACTAC AGAGATGGCA GCGACGACGT CGGCGGCGGG 
TCCGCGGATC TGGGCGAGCT CAGCCTTCGG CCACAGAACG ACACGGCCGC CTGGTTGCAG 
CAGCAGGAGA AGGCGGCCAA GGTGGCAGCA GCTGCGGCAG CGGACCAGGG CCTCAACCTA 
CAGGGCCCGG GCCACCATCA GATGGGCGGT AATGGCGGAG CAGGAGGAGG GTCGGGCGGA 
GCTGTGCCCC TGCCCATACT ATCGCTGATG CCCTCGTTCA TGCGTAGCGG AGCTCCGATG 
CCCGCCAGTG GAGCGACAGC GGCCAGCATC TTGGCCGGCG GATCGTTGGA CACTTCTGCG 
TTGCCCAACG CTCCCTACCA GAACGGAATC CTGGGTGCTC GGTCTGCCTC GCCAGCTGTG 
GTGGGCAGTG GAAGCTTCAG GGCGGCCACT GCCATTCCCA AGCGACCACC GACCTCCTCG 
TCGCCCCCAC AATCTGGCCT AGGCATTGGA GGATCACCCA CTCCCCCACA ATCCCAGCAG 
CAGGCCAACG GAGGCGCTGG CGGAGCCGGG CGCAAGGATA AGGACTACGT GGTGGAGCCG 
GAGGTGGCCC AAATGCAGCG TCCTATCATA CGCGAAGAGG ACTTGGAGCG CCTCAACGCC 
ATCGCCAAGG ACGATAGCTG GACCAAGCAG GACGACATTG ACTTCACGAA GAAACTAGCA 
TTCTCGGACG ATGAGTCCAC TCCGGAAGAG CACCAAGGCC AGGGCAAGCA GCACAGCTCC 
GCCCAACTGC AGGCGCAAGG ATCCTCCTCC TCAACCGATC AACGAAAGCT GTCCGCTTCC 
ACAACCAGTT GGCAGCGCGG CAAGGAGAGC ACCGATCGCG ACCAGCCATC GGAAGGCATT 
CAGGATCAGC AGCAGCAGCT TCAGCAGCAA CAGCAGCAGC AAGAGGTTCG CCAACAGAAT 
GGCCACCGCG GTAGCTCTGG CAACGTTGGA GCACCAGTGG CCGGTGGAAT TGCCTTGGAT 
GCCAGCGTGT ACGAGCGAGT AAAGCAGCGC AAGGAGGAGG AAGAGCGTCG CGAGATGGAA 
CGCAAGCAGG CGGCTGCTAA GAAGTTGCAA GAACTGGAAA TGAAGATGAA TAGCAAAAAG 
GCAGCGGCTG CGGCTCTGGC TGGAGCCGAG GGCGGCGTGT CCTCTGCCTC AGTCCCGTTG 
AGCAATGAGG TGCCACCCAC GTCCGCACCG CTAGGCAATG CCAGCGAAGA TGAGGGCGGA 
GGCCAAGGCG GCGGCGGAGG GTTGAATCGT CGACCCCGGG GAAGCATCTC TAGCTTGGGA 
AGCGGCGTAA CACCCACGGG AGGAGCTGGC GGCGGCGGCG GAGGAGTTTC GTCATCCTCG 
TCAGCAGGCG GCGACTATGG TCAAAAGAAC TTCCTTACCC ATTTCCAATC AAATTTACCA 
CCACGTTTCC AGCGCCAGCA ACAGCAACAA CAGCAGCAGC TGCCGCGCTT GGAGAAGAGC 
GCCTCGGCAA GCAGTGCATT CGATAGCAAT TCCCGCTACC TCCAGAAGGG AGGAGTAAGC 
GGAGGAGGCG GTGGTGTAAG TGGAGGAGCG CAAGCCAATG CTGGCCAGGG TCGCAGCATC 
TCCGGCGGTT ATGTGCAGCG TGGTGCAAGT GTCGGAGGAA GCAGCGGATA CGGACGAAAT 
CGTCACGACA GCAGCAGTGC TCGGGAGGAT CAGGATCTGG ATCAGCAGCG TTTCACCCGT 
GGCCAGGACT ATCGGCAGGC ACAAGCTCTG CCGCGCAGCA TATCTGAGAA CTCGCACCGT 
AAGACCAGCG TCTCATCGGC TGGCGACGAT GTGCTGGGTG GCTCGTGCTC CTCATGGGCG 
GAGCAAACGG ATGCCGAGCA GAAGGTGCAC CATCGGCACC GCGAGGAGAG CTTCTCCTCG 
TCGCACAGCC ATGACTCGGC AGTGCAAATA AAGATCCTGC AACGCCCGGC CCGACAGCCG 
AGCCTCAGTG AGGAGTCCTC GACCCAGCAG AAACAGCTGC CCGCCTCGCC GCAGCAGCAA 
AAGACGCTGA CGACAGATCC CATGCCTACC CAGATTCTGC GACGCAGCGT GGATATCGAT 
AAGTCCGGTG ATGAGCGCGC GGAGGACAAG TCTGCATTGG AATCTGATGA CAAGGCGAAG 
TCGGCTGGCA AGGTGGAGGA GGACAAGAGC GGCAATAAGC GGGTAAGTCC CAGGAGCGGA 
GCTGCTTGCG GAGGTCGAGG TGGCCGTGGT TATGCCGGCA GCTCTGGTGG ATCATCCGTC 
GGATCGGGCA GTAGCTACCG CGGCCAGAGA AATGCCAGCG ATTGGGGAAG CAGTCGTGGA 
AGCGGCGGCG CCGGCGGCAG GCGCTACTAC GGCAGCGGCG GTGAGCAATC GGAGCACTCC 
GAGGATGTGG ACGAGGAGTG TTACAGCAGT GGAGGAGGCG GAGCCGCTCG TCGCAGCCCA 
AAGGAATCGG GCAACGGTGC GCCCAATTCC GGTGCGTCGG CAAACAAGGC AGGATTCTCG 
CCACGCGGCG AACCATCTAG ACGTGGTCGA GGCGGCCTCT CTAGCGGAGG AGGCGCCTCT 
GCTTCTTGTT ACCGTCGACA GCCCGTGGGC ACTGGATCCG CTGGTGGATC TGGTTCGGGC 
GGATCGGGCC GCAGCTATGG CAGACTTGGC TACGAGGAAT ATGGCAAGCA ACAGCGAACT 
TCCGAGTCGG AGACGGACCT AGACAAGACC AAGCAGAACC AGCTGGCACT CAGCGCCGGC 
CTGCACAAAC CAAGCAAGGA TGACAAGGAT TTGCCTGCGC TTGGGGGCGA GGAGAAGGAC 
AAGACTGCCG CCCTGGCGCC TGCCCATAAC ACTGCCCTGC TAGCAAAGGA TGAATTACAG 
AAAGATGCAG CGGGTCGCCT GCCGTTGCCT CCCGGCCTCG CAGCACCTGG CTCTGCAGTG 
GGATCCGTTA CTGCCACATC CTCATCGGTC GTAGTTCCCG GTGCAGCGGA CAAGAAGAAG 
ATTGAATCCT GCGCCGTTGT CGGCGGTGAG AAGGTGACGG GTAGCAAGTT GCCCGCCATT 
GGGGAAAAGA CTTCGCTCGG CGCCGTTGGC TCGGGTCCAT CAACCAGTCT TCTACTGGAC 
GCCGGCGCAC CCGTCAACAC GATTATATTT GAGAACTCCA CGTACAAGCA GCAGGCAGCT 
GCCGCTTCCG TGGTAACCAA ACCCGGAACG CTCTCGGTGT CAAGCAGTGG CGCCACCATG 
ACCGTGGACA GCCTGTCCAG CGCACTCTCC CAAATGAGTT TCGGCGGCAA GGCGACGGCG 
GCGGGCTCTG GCGATGATTC GCAGGATATG AAACTAGGAT TTAGCTTCGG TGACGATCCC 
ACCACACCCC TCAAGGTTGT GGATAACATC GATAAAACGG CCAGTCAGCA GCAGCAACAG 
CAGCAGCAAC AACACTCTCA ACAGCAACAA CAACAGCAGC AGCAGCAAAA CAATTCGACG 
GCGGATCTTA ACATGAAGAT AGCCAGCGTA AAGAAGGTTT GGGAGTCAGC CACGCCCATG 
TCCGACGGCC CATCGCCAGC ACAGGTGCAG CAGCAACAGC AACAGCAGCC CCAACAGCAA 
CAACAGTCCC AGGTGCAGGT GGCGGCGCAG CAGCAACAAC AGCAGGTGGT GGGACCGCAG 
CAGGCAGGTG ACGACGGCAG TGGTCACATG AGCGCCGCCT CCTTTGTGGC AGCCGTGGCC 
GCCTCACAGC ATCAGCAGCA ACACCAGCAA ATGCCGCACT ACGCGGTGTC GGCGGTGCAC 
ATGCAGAGCC ACCACCACCA ACAGCAGCAG CAACAGCAGC AGGCAGCGGT GGCGGCAGCG 
CAGGCGCAGC ACCACCAGCA GCAACAGGCA CAGGCGCACC ACCAGCACCA TGCACTTTCC 
TCGCCGGGCC CGGTGCCCGG TAGTCATGGC CATGGCTACA GTCATGGATC GCCGTTCGAC 
GCTGGATCGT TGGAGCAACA GTTCCAGCAA GAGGATTATC CCAGTCCACA GCAGCAACAG 
CAGGCGCACC AGAAGACCAA GCAGTTGCAG CAGCAGCAGC ATCTGGGCAT GTCACCGCCG 
CCCAGCCAGC AGCAACAGCA GCAGCACTCG CAACAGCAGC AGCAAGCACA GCACCAGCAA 
CAACAGCAGC AGGCCCATCA GCAGCAGCAA CAGCAGCAGC AGCCTTCATT CTACCAGGCG 
TCACCGCAGT TCGGCGTTGG AGGCATTCCC ACTATCCCCA GTCCGCCGGC CGTGGTGTAC 
AACTCGTCGC AGATGCCGCC ACCGTCGCAG GGGGGTAATC TGTACGCTCC ATTCCATTCG 
CTCGATCATT CGAGCCGTTC GCCCGCCTAC TCGGCGGCGG CGGCCGCCCA CAGCTACCCG 
AACCCTTATG CGGCAGCAGC AGCGGCGGCG GCGGCAGCGG CCGCTGGCGG AGGTCCCTTC 
AATGTCAACG TGAACGCATA TGCGATGCAG ACTCCGCACG GCGGCAGCCT ACCGCCCACA 
GCGCCCACAC CGGACATGTA CTCGAACCTC TCTTCGCAGT TTCGATTGGG CGGTGGTCCG 
GGTCCCGGAC CCGGTCCCTT TGGCCAGCCC AGCTCGCAGC AGTTAAGCAA TCCGAGCAAT 
CATGCTGCGG TGGCTATAAT TTCGTCAAGC AGCAACTCCA TGATGTCGTC AGGTGCGGCC 
AAACCGCCGC AGAGCCAGCA GACAATCGGT GCCATTGGCT CCAAGTCGGC AGGTAGCGGT 
GGCGGTCCAG GACCAGGACC TGGTCCCTAT GCCACCACCC AGACGTATAT GAATCTTTAT 
GCGGCTCCAC CGCCGCAACA TCCAGGACAA GGTGGACCAC CGCCGGGTGC CCATCAGCTG 
CAGTCGAACA GTTTCTACTC GAATTCGGCG CCAGGCGGTC CGAGCGGACC ACAGTTCTAC 
GGTGGTCCGC CGCCGCAGGG CAATGGTGGA GCGCAGAGCT ATGGACTGGC CACCGCGGCC 
GGCATGTATG GCGGCCATCA GGGCGGACCG CCCGGCTCCA ATGGGCCGCC GGGACCGCCG 
CAGTCGCAGC ACACGATGGG CACGTTCAAC ACGCCGTTCA TGAACTCGCA GCTGCTGACG 
GCGGCCAGCA TTAATCAGTA CCGCGGCGGA CCAACGCCTG GAGCGGCTTA TCTCAAGGGT 
AACCAGGGCC AGACACACAT GCAAGACTCG ATGGGTCGTC AGCTGAAGAG TCCGCTGAGC 
GCTGACATGA GCCTCGGTCT GGCCAAGCAG GTGCAGTCGC AGCCCAGTCC ACCGCATCAC 
AAGAACTACG GCGCGTGGGA TCTGCAGAAC CAGGTGCTTC AGCAGCAGCA GCAGCAATCT 
CAGCAGCAAC AGCAGCAGCA ACAGCGCCAG GTCGGCGGCG GCAATGGTCC GAGCATGAAC 
GCCTTGGGCG GTCGCGGCAG TGGAGCGGTG GGCAGCGGCA GCGGCAACGG CGGAGGAGGA 
GGAGGAGGAG CTGGTGGGTC CCAAGTGGGC GGCAATGGCG GCGGTAACGG CGTTGGCAGC 
GTGGGCCAAA GTGGCGGCGG CGGCCAGGGA CGCTATCCCA CGCCCATTCA GCGGCCCAAC 
AACTATCCGC AGCATCCGCA ACAACAGCAG CAACAACAGC AGCAACAGCA ACAGCAGAGA 
GAGCAGGCGG CAGCTGCCGC CGTGGCGGCT CAGCGGGCAC AGAATATGCG ACAGGTGACC 
GGTGCCGGCG GAGGCGTGGC CGGAAGTGCA GGTGGCGGTG GTAGTCAAAC ACCAGTGGGA 
CCGGCCGGCG GCAATGGTGG CCCCGGTGGC GCAGGAGGAG GCGGTGGAGC TGGCGGTGCA 
GCAGGATCAG GACCCGGAAA GCCCTACTAT GCGAATAACG CTAGCGGAGC GGCCGTTGCG 
GCCAATCGAG GTGAGTGGCA TGCGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nocte ( NM_167231.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727425.3 Drosophila melanogaster no circadian temperature entrainment (nocte), transcript variant B, mRNA.

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CloneID	OFa01233
Clone ID Related Accession (Same CDS sequence)	NM_001258685.2 , NM_167231.5 , NM_132403.5
Accession Version	NM_167231.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	no circadian temperature entrainment, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167231.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTACAC TGGGGGGAAG TAGGGGAGAG CGAAATGCCA AGCCCAAATT CACAGCGTTA 
GATATAAATC GCATGTACAA GAATAGCCGC GGCGAGTCAA GTGAACCATC TGCACAAAAG 
AATCAAGTGC CACGTAAACA TGGAATGCAA ATCTTGGGCA AAGTGCCGTC CGCTAGGCGA 
CCACCAGCCA ATTTGCCATC CCTGAAGGCC GAGACCCACA CCAGCAGCAA CAACAATCTC 
TTAGTCTCAG TCGAAGGAGC CACATCTGGT GGGGCGGGCG CAGGCGGAGC AGCGGGATCT 
GGCGCGGCAG GAACCAACAC TAGCCACAAC AACACCACTG CCCACAGCGG AAGTTCAGGA 
ACGAGCGGCG GAGCGGGATC AGGCGGCGGA GGAACTGCGT CTTCATCTGC TGGTGGCCAT 
CTATCCCAGC AGCTGACTCA CAGCCAGCAT CAGCACAATA ATAACGTCGC TCTCGGCCCT 
AACGCCGGAA AGTTTGTCAA CAGCAACAAC GGCAGCAGCA ACAACAACGC CGGCAGCTCA 
GGCAGCGGCA ACAAGAACTT CAAATTAATC AACAACTCCG TGGGCAGTTC CGGCGGAGGC 
GGACAAGGAG GGTCGGGCGG CGGCGGATCG TCAGGAGTAG GAGGTGGATC AGGGTCAGGA 
TCGGGCGGAC ACTCAGTCAA CTCACCCAAG ACATGGTCTG CAATTACCAC AGGACACGAG 
CGGGGCTCGG GCGGTCAGAA CTATGGACGC CAGCAACAGC AGGTGGTCCC CCATTATCAG 
AGTCCACAGT TCCAGTACGA ATTTCCCACG CTGGACGGCA CAGTTGGCAG CGGCGCCGGC 
GGAGGATCGG GAGCGGGTGT GGGTTCAGGA TCTGGAGCGG GCAAGAGCCA TTACCAGAAT 
CAGGTGCACC ATGCCGGCCA CCAAAACCAT CACCAGCATC AGCAGCACCA CCAGCAGCAT 
CAACAGCACC ACCACCAGCA GCAACAGCAC CATCGGGACT ACCACCATGG CCACGGGCGC 
CAGTATGGAC ACCACTCGCA TCGCGACTAC AGAGATGGCA GCGACGACGT CGGCGGCGGG 
TCCGCGGATC TGGGCGAGCT CAGCCTTCGG CCACAGAACG ACACGGCCGC CTGGTTGCAG 
CAGCAGGAGA AGGCGGCCAA GGTGGCAGCA GCTGCGGCAG CGGACCAGGG CCTCAACCTA 
CAGGGCCCGG GCCACCATCA GATGGGCGGT AATGGCGGAG CAGGAGGAGG GTCGGGCGGA 
GCTGTGCCCC TGCCCATACT ATCGCTGATG CCCTCGTTCA TGCGTAGCGG AGCTCCGATG 
CCCGCCAGTG GAGCGACAGC GGCCAGCATC TTGGCCGGCG GATCGTTGGA CACTTCTGCG 
TTGCCCAACG CTCCCTACCA GAACGGAATC CTGGGTGCTC GGTCTGCCTC GCCAGCTGTG 
GTGGGCAGTG GAAGCTTCAG GGCGGCCACT GCCATTCCCA AGCGACCACC GACCTCCTCG 
TCGCCCCCAC AATCTGGCCT AGGCATTGGA GGATCACCCA CTCCCCCACA ATCCCAGCAG 
CAGGCCAACG GAGGCGCTGG CGGAGCCGGG CGCAAGGATA AGGACTACGT GGTGGAGCCG 
GAGGTGGCCC AAATGCAGCG TCCTATCATA CGCGAAGAGG ACTTGGAGCG CCTCAACGCC 
ATCGCCAAGG ACGATAGCTG GACCAAGCAG GACGACATTG ACTTCACGAA GAAACTAGCA 
TTCTCGGACG ATGAGTCCAC TCCGGAAGAG CACCAAGGCC AGGGCAAGCA GCACAGCTCC 
GCCCAACTGC AGGCGCAAGG ATCCTCCTCC TCAACCGATC AACGAAAGCT GTCCGCTTCC 
ACAACCAGTT GGCAGCGCGG CAAGGAGAGC ACCGATCGCG ACCAGCCATC GGAAGGCATT 
CAGGATCAGC AGCAGCAGCT TCAGCAGCAA CAGCAGCAGC AAGAGGTTCG CCAACAGAAT 
GGCCACCGCG GTAGCTCTGG CAACGTTGGA GCACCAGTGG CCGGTGGAAT TGCCTTGGAT 
GCCAGCGTGT ACGAGCGAGT AAAGCAGCGC AAGGAGGAGG AAGAGCGTCG CGAGATGGAA 
CGCAAGCAGG CGGCTGCTAA GAAGTTGCAA GAACTGGAAA TGAAGATGAA TAGCAAAAAG 
GCAGCGGCTG CGGCTCTGGC TGGAGCCGAG GGCGGCGTGT CCTCTGCCTC AGTCCCGTTG 
AGCAATGAGG TGCCACCCAC GTCCGCACCG CTAGGCAATG CCAGCGAAGA TGAGGGCGGA 
GGCCAAGGCG GCGGCGGAGG GTTGAATCGT CGACCCCGGG GAAGCATCTC TAGCTTGGGA 
AGCGGCGTAA CACCCACGGG AGGAGCTGGC GGCGGCGGCG GAGGAGTTTC GTCATCCTCG 
TCAGCAGGCG GCGACTATGG TCAAAAGAAC TTCCTTACCC ATTTCCAATC AAATTTACCA 
CCACGTTTCC AGCGCCAGCA ACAGCAACAA CAGCAGCAGC TGCCGCGCTT GGAGAAGAGC 
GCCTCGGCAA GCAGTGCATT CGATAGCAAT TCCCGCTACC TCCAGAAGGG AGGAGTAAGC 
GGAGGAGGCG GTGGTGTAAG TGGAGGAGCG CAAGCCAATG CTGGCCAGGG TCGCAGCATC 
TCCGGCGGTT ATGTGCAGCG TGGTGCAAGT GTCGGAGGAA GCAGCGGATA CGGACGAAAT 
CGTCACGACA GCAGCAGTGC TCGGGAGGAT CAGGATCTGG ATCAGCAGCG TTTCACCCGT 
GGCCAGGACT ATCGGCAGGC ACAAGCTCTG CCGCGCAGCA TATCTGAGAA CTCGCACCGT 
AAGACCAGCG TCTCATCGGC TGGCGACGAT GTGCTGGGTG GCTCGTGCTC CTCATGGGCG 
GAGCAAACGG ATGCCGAGCA GAAGGTGCAC CATCGGCACC GCGAGGAGAG CTTCTCCTCG 
TCGCACAGCC ATGACTCGGC AGTGCAAATA AAGATCCTGC AACGCCCGGC CCGACAGCCG 
AGCCTCAGTG AGGAGTCCTC GACCCAGCAG AAACAGCTGC CCGCCTCGCC GCAGCAGCAA 
AAGACGCTGA CGACAGATCC CATGCCTACC CAGATTCTGC GACGCAGCGT GGATATCGAT 
AAGTCCGGTG ATGAGCGCGC GGAGGACAAG TCTGCATTGG AATCTGATGA CAAGGCGAAG 
TCGGCTGGCA AGGTGGAGGA GGACAAGAGC GGCAATAAGC GGGTAAGTCC CAGGAGCGGA 
GCTGCTTGCG GAGGTCGAGG TGGCCGTGGT TATGCCGGCA GCTCTGGTGG ATCATCCGTC 
GGATCGGGCA GTAGCTACCG CGGCCAGAGA AATGCCAGCG ATTGGGGAAG CAGTCGTGGA 
AGCGGCGGCG CCGGCGGCAG GCGCTACTAC GGCAGCGGCG GTGAGCAATC GGAGCACTCC 
GAGGATGTGG ACGAGGAGTG TTACAGCAGT GGAGGAGGCG GAGCCGCTCG TCGCAGCCCA 
AAGGAATCGG GCAACGGTGC GCCCAATTCC GGTGCGTCGG CAAACAAGGC AGGATTCTCG 
CCACGCGGCG AACCATCTAG ACGTGGTCGA GGCGGCCTCT CTAGCGGAGG AGGCGCCTCT 
GCTTCTTGTT ACCGTCGACA GCCCGTGGGC ACTGGATCCG CTGGTGGATC TGGTTCGGGC 
GGATCGGGCC GCAGCTATGG CAGACTTGGC TACGAGGAAT ATGGCAAGCA ACAGCGAACT 
TCCGAGTCGG AGACGGACCT AGACAAGACC AAGCAGAACC AGCTGGCACT CAGCGCCGGC 
CTGCACAAAC CAAGCAAGGA TGACAAGGAT TTGCCTGCGC TTGGGGGCGA GGAGAAGGAC 
AAGACTGCCG CCCTGGCGCC TGCCCATAAC ACTGCCCTGC TAGCAAAGGA TGAATTACAG 
AAAGATGCAG CGGGTCGCCT GCCGTTGCCT CCCGGCCTCG CAGCACCTGG CTCTGCAGTG 
GGATCCGTTA CTGCCACATC CTCATCGGTC GTAGTTCCCG GTGCAGCGGA CAAGAAGAAG 
ATTGAATCCT GCGCCGTTGT CGGCGGTGAG AAGGTGACGG GTAGCAAGTT GCCCGCCATT 
GGGGAAAAGA CTTCGCTCGG CGCCGTTGGC TCGGGTCCAT CAACCAGTCT TCTACTGGAC 
GCCGGCGCAC CCGTCAACAC GATTATATTT GAGAACTCCA CGTACAAGCA GCAGGCAGCT 
GCCGCTTCCG TGGTAACCAA ACCCGGAACG CTCTCGGTGT CAAGCAGTGG CGCCACCATG 
ACCGTGGACA GCCTGTCCAG CGCACTCTCC CAAATGAGTT TCGGCGGCAA GGCGACGGCG 
GCGGGCTCTG GCGATGATTC GCAGGATATG AAACTAGGAT TTAGCTTCGG TGACGATCCC 
ACCACACCCC TCAAGGTTGT GGATAACATC GATAAAACGG CCAGTCAGCA GCAGCAACAG 
CAGCAGCAAC AACACTCTCA ACAGCAACAA CAACAGCAGC AGCAGCAAAA CAATTCGACG 
GCGGATCTTA ACATGAAGAT AGCCAGCGTA AAGAAGGTTT GGGAGTCAGC CACGCCCATG 
TCCGACGGCC CATCGCCAGC ACAGGTGCAG CAGCAACAGC AACAGCAGCC CCAACAGCAA 
CAACAGTCCC AGGTGCAGGT GGCGGCGCAG CAGCAACAAC AGCAGGTGGT GGGACCGCAG 
CAGGCAGGTG ACGACGGCAG TGGTCACATG AGCGCCGCCT CCTTTGTGGC AGCCGTGGCC 
GCCTCACAGC ATCAGCAGCA ACACCAGCAA ATGCCGCACT ACGCGGTGTC GGCGGTGCAC 
ATGCAGAGCC ACCACCACCA ACAGCAGCAG CAACAGCAGC AGGCAGCGGT GGCGGCAGCG 
CAGGCGCAGC ACCACCAGCA GCAACAGGCA CAGGCGCACC ACCAGCACCA TGCACTTTCC 
TCGCCGGGCC CGGTGCCCGG TAGTCATGGC CATGGCTACA GTCATGGATC GCCGTTCGAC 
GCTGGATCGT TGGAGCAACA GTTCCAGCAA GAGGATTATC CCAGTCCACA GCAGCAACAG 
CAGGCGCACC AGAAGACCAA GCAGTTGCAG CAGCAGCAGC ATCTGGGCAT GTCACCGCCG 
CCCAGCCAGC AGCAACAGCA GCAGCACTCG CAACAGCAGC AGCAAGCACA GCACCAGCAA 
CAACAGCAGC AGGCCCATCA GCAGCAGCAA CAGCAGCAGC AGCCTTCATT CTACCAGGCG 
TCACCGCAGT TCGGCGTTGG AGGCATTCCC ACTATCCCCA GTCCGCCGGC CGTGGTGTAC 
AACTCGTCGC AGATGCCGCC ACCGTCGCAG GGGGGTAATC TGTACGCTCC ATTCCATTCG 
CTCGATCATT CGAGCCGTTC GCCCGCCTAC TCGGCGGCGG CGGCCGCCCA CAGCTACCCG 
AACCCTTATG CGGCAGCAGC AGCGGCGGCG GCGGCAGCGG CCGCTGGCGG AGGTCCCTTC 
AATGTCAACG TGAACGCATA TGCGATGCAG ACTCCGCACG GCGGCAGCCT ACCGCCCACA 
GCGCCCACAC CGGACATGTA CTCGAACCTC TCTTCGCAGT TTCGATTGGG CGGTGGTCCG 
GGTCCCGGAC CCGGTCCCTT TGGCCAGCCC AGCTCGCAGC AGTTAAGCAA TCCGAGCAAT 
CATGCTGCGG TGGCTATAAT TTCGTCAAGC AGCAACTCCA TGATGTCGTC AGGTGCGGCC 
AAACCGCCGC AGAGCCAGCA GACAATCGGT GCCATTGGCT CCAAGTCGGC AGGTAGCGGT 
GGCGGTCCAG GACCAGGACC TGGTCCCTAT GCCACCACCC AGACGTATAT GAATCTTTAT 
GCGGCTCCAC CGCCGCAACA TCCAGGACAA GGTGGACCAC CGCCGGGTGC CCATCAGCTG 
CAGTCGAACA GTTTCTACTC GAATTCGGCG CCAGGCGGTC CGAGCGGACC ACAGTTCTAC 
GGTGGTCCGC CGCCGCAGGG CAATGGTGGA GCGCAGAGCT ATGGACTGGC CACCGCGGCC 
GGCATGTATG GCGGCCATCA GGGCGGACCG CCCGGCTCCA ATGGGCCGCC GGGACCGCCG 
CAGTCGCAGC ACACGATGGG CACGTTCAAC ACGCCGTTCA TGAACTCGCA GCTGCTGACG 
GCGGCCAGCA TTAATCAGTA CCGCGGCGGA CCAACGCCTG GAGCGGCTTA TCTCAAGGGT 
AACCAGGGCC AGACACACAT GCAAGACTCG ATGGGTCGTC AGCTGAAGAG TCCGCTGAGC 
GCTGACATGA GCCTCGGTCT GGCCAAGCAG GTGCAGTCGC AGCCCAGTCC ACCGCATCAC 
AAGAACTACG GCGCGTGGGA TCTGCAGAAC CAGGTGCTTC AGCAGCAGCA GCAGCAATCT 
CAGCAGCAAC AGCAGCAGCA ACAGCGCCAG GTCGGCGGCG GCAATGGTCC GAGCATGAAC 
GCCTTGGGCG GTCGCGGCAG TGGAGCGGTG GGCAGCGGCA GCGGCAACGG CGGAGGAGGA 
GGAGGAGGAG CTGGTGGGTC CCAAGTGGGC GGCAATGGCG GCGGTAACGG CGTTGGCAGC 
GTGGGCCAAA GTGGCGGCGG CGGCCAGGGA CGCTATCCCA CGCCCATTCA GCGGCCCAAC 
AACTATCCGC AGCATCCGCA ACAACAGCAG CAACAACAGC AGCAACAGCA ACAGCAGAGA 
GAGCAGGCGG CAGCTGCCGC CGTGGCGGCT CAGCGGGCAC AGAATATGCG ACAGGTGACC 
GGTGCCGGCG GAGGCGTGGC CGGAAGTGCA GGTGGCGGTG GTAGTCAAAC ACCAGTGGGA 
CCGGCCGGCG GCAATGGTGG CCCCGGTGGC GCAGGAGGAG GCGGTGGAGC TGGCGGTGCA 
GCAGGATCAG GACCCGGAAA GCCCTACTAT GCGAATAACG CTAGCGGAGC GGCCGTTGCG 
GCCAATCGAG GTGAGTGGCA TGCGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727425.3
CDS	798..7727
Translation	MSTLGGSRGERNAKPKFTALDINRMYKNSRGESSEPSAQKNQVPRKHGMQILGKVPSARRPPANLPSLKAETHTSSNNNLLVSVEGATSGGAGAGGAAGSGAAGTNTSHNNTTAHSGSSGTSGGAGSGGGGTASSSAGGHLSQQLTHSQHQHNNNVALGPNAGKFVNSNNGSSNNNAGSSGSGNKNFKLINNSVGSSGGGGQGGSGGGGSSGVGGGSGSGSGGHSVNSPKTWSAITTGHERGSGGQNYGRQQQQVVPHYQSPQFQYEFPTLDGTVGSGAGGGSGAGVGSGSGAGKSHYQNQVHHAGHQNHHQHQQHHQQHQQHHHQQQQHHRDYHHGHGRQYGHHSHRDYRDGSDDVGGGSADLGELSLRPQNDTAAWLQQQEKAAKVAAAAAADQGLNLQGPGHHQMGGNGGAGGGSGGAVPLPILSLMPSFMRSGAPMPASGATAASILAGGSLDTSALPNAPYQNGILGARSASPAVVGSGSFRAATAIPKRPPTSSSPPQSGLGIGGSPTPPQSQQQANGGAGGAGRKDKDYVVEPEVAQMQRPIIREEDLERLNAIAKDDSWTKQDDIDFTKKLAFSDDESTPEEHQGQGKQHSSAQLQAQGSSSSTDQRKLSASTTSWQRGKESTDRDQPSEGIQDQQQQLQQQQQQQEVRQQNGHRGSSGNVGAPVAGGIALDASVYERVKQRKEEEERREMERKQAAAKKLQELEMKMNSKKAAAAALAGAEGGVSSASVPLSNEVPPTSAPLGNASEDEGGGQGGGGGLNRRPRGSISSLGSGVTPTGGAGGGGGGVSSSSSAGGDYGQKNFLTHFQSNLPPRFQRQQQQQQQQLPRLEKSASASSAFDSNSRYLQKGGVSGGGGGVSGGAQANAGQGRSISGGYVQRGASVGGSSGYGRNRHDSSSAREDQDLDQQRFTRGQDYRQAQALPRSISENSHRKTSVSSAGDDVLGGSCSSWAEQTDAEQKVHHRHREESFSSSHSHDSAVQIKILQRPARQPSLSEESSTQQKQLPASPQQQKTLTTDPMPTQILRRSVDIDKSGDERAEDKSALESDDKAKSAGKVEEDKSGNKRVSPRSGAACGGRGGRGYAGSSGGSSVGSGSSYRGQRNASDWGSSRGSGGAGGRRYYGSGGEQSEHSEDVDEECYSSGGGGAARRSPKESGNGAPNSGASANKAGFSPRGEPSRRGRGGLSSGGGASASCYRRQPVGTGSAGGSGSGGSGRSYGRLGYEEYGKQQRTSESETDLDKTKQNQLALSAGLHKPSKDDKDLPALGGEEKDKTAALAPAHNTALLAKDELQKDAAGRLPLPPGLAAPGSAVGSVTATSSSVVVPGAADKKKIESCAVVGGEKVTGSKLPAIGEKTSLGAVGSGPSTSLLLDAGAPVNTIIFENSTYKQQAAAASVVTKPGTLSVSSSGATMTVDSLSSALSQMSFGGKATAAGSGDDSQDMKLGFSFGDDPTTPLKVVDNIDKTASQQQQQQQQQHSQQQQQQQQQQNNSTADLNMKIASVKKVWESATPMSDGPSPAQVQQQQQQQPQQQQQSQVQVAAQQQQQQVVGPQQAGDDGSGHMSAASFVAAVAASQHQQQHQQMPHYAVSAVHMQSHHHQQQQQQQQAAVAAAQAQHHQQQQAQAHHQHHALSSPGPVPGSHGHGYSHGSPFDAGSLEQQFQQEDYPSPQQQQQAHQKTKQLQQQQHLGMSPPPSQQQQQQHSQQQQQAQHQQQQQQAHQQQQQQQQPSFYQASPQFGVGGIPTIPSPPAVVYNSSQMPPPSQGGNLYAPFHSLDHSSRSPAYSAAAAAHSYPNPYAAAAAAAAAAAAGGGPFNVNVNAYAMQTPHGGSLPPTAPTPDMYSNLSSQFRLGGGPGPGPGPFGQPSSQQLSNPSNHAAVAIISSSSNSMMSSGAAKPPQSQQTIGAIGSKSAGSGGGPGPGPGPYATTQTYMNLYAAPPPQHPGQGGPPPGAHQLQSNSFYSNSAPGGPSGPQFYGGPPPQGNGGAQSYGLATAAGMYGGHQGGPPGSNGPPGPPQSQHTMGTFNTPFMNSQLLTAASINQYRGGPTPGAAYLKGNQGQTHMQDSMGRQLKSPLSADMSLGLAKQVQSQPSPPHHKNYGAWDLQNQVLQQQQQQSQQQQQQQQRQVGGGNGPSMNALGGRGSGAVGSGSGNGGGGGGGAGGSQVGGNGGGNGVGSVGQSGGGGQGRYPTPIQRPNNYPQHPQQQQQQQQQQQQQREQAAAAAVAAQRAQNMRQVTGAGGGVAGSAGGGGSQTPVGPAGGNGGPGGAGGGGGAGGAAGSGPGKPYYANNASGAAVAANRGEWHAN

Target ORF information:

RefSeq Version	NM_167231.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster no circadian temperature entrainment (nocte), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167231.5

ORF Insert Sequence:

ATGAGTACAC TGGGGGGAAG TAGGGGAGAG CGAAATGCCA AGCCCAAATT CACAGCGTTA 
GATATAAATC GCATGTACAA GAATAGCCGC GGCGAGTCAA GTGAACCATC TGCACAAAAG 
AATCAAGTGC CACGTAAACA TGGAATGCAA ATCTTGGGCA AAGTGCCGTC CGCTAGGCGA 
CCACCAGCCA ATTTGCCATC CCTGAAGGCC GAGACCCACA CCAGCAGCAA CAACAATCTC 
TTAGTCTCAG TCGAAGGAGC CACATCTGGT GGGGCGGGCG CAGGCGGAGC AGCGGGATCT 
GGCGCGGCAG GAACCAACAC TAGCCACAAC AACACCACTG CCCACAGCGG AAGTTCAGGA 
ACGAGCGGCG GAGCGGGATC AGGCGGCGGA GGAACTGCGT CTTCATCTGC TGGTGGCCAT 
CTATCCCAGC AGCTGACTCA CAGCCAGCAT CAGCACAATA ATAACGTCGC TCTCGGCCCT 
AACGCCGGAA AGTTTGTCAA CAGCAACAAC GGCAGCAGCA ACAACAACGC CGGCAGCTCA 
GGCAGCGGCA ACAAGAACTT CAAATTAATC AACAACTCCG TGGGCAGTTC CGGCGGAGGC 
GGACAAGGAG GGTCGGGCGG CGGCGGATCG TCAGGAGTAG GAGGTGGATC AGGGTCAGGA 
TCGGGCGGAC ACTCAGTCAA CTCACCCAAG ACATGGTCTG CAATTACCAC AGGACACGAG 
CGGGGCTCGG GCGGTCAGAA CTATGGACGC CAGCAACAGC AGGTGGTCCC CCATTATCAG 
AGTCCACAGT TCCAGTACGA ATTTCCCACG CTGGACGGCA CAGTTGGCAG CGGCGCCGGC 
GGAGGATCGG GAGCGGGTGT GGGTTCAGGA TCTGGAGCGG GCAAGAGCCA TTACCAGAAT 
CAGGTGCACC ATGCCGGCCA CCAAAACCAT CACCAGCATC AGCAGCACCA CCAGCAGCAT 
CAACAGCACC ACCACCAGCA GCAACAGCAC CATCGGGACT ACCACCATGG CCACGGGCGC 
CAGTATGGAC ACCACTCGCA TCGCGACTAC AGAGATGGCA GCGACGACGT CGGCGGCGGG 
TCCGCGGATC TGGGCGAGCT CAGCCTTCGG CCACAGAACG ACACGGCCGC CTGGTTGCAG 
CAGCAGGAGA AGGCGGCCAA GGTGGCAGCA GCTGCGGCAG CGGACCAGGG CCTCAACCTA 
CAGGGCCCGG GCCACCATCA GATGGGCGGT AATGGCGGAG CAGGAGGAGG GTCGGGCGGA 
GCTGTGCCCC TGCCCATACT ATCGCTGATG CCCTCGTTCA TGCGTAGCGG AGCTCCGATG 
CCCGCCAGTG GAGCGACAGC GGCCAGCATC TTGGCCGGCG GATCGTTGGA CACTTCTGCG 
TTGCCCAACG CTCCCTACCA GAACGGAATC CTGGGTGCTC GGTCTGCCTC GCCAGCTGTG 
GTGGGCAGTG GAAGCTTCAG GGCGGCCACT GCCATTCCCA AGCGACCACC GACCTCCTCG 
TCGCCCCCAC AATCTGGCCT AGGCATTGGA GGATCACCCA CTCCCCCACA ATCCCAGCAG 
CAGGCCAACG GAGGCGCTGG CGGAGCCGGG CGCAAGGATA AGGACTACGT GGTGGAGCCG 
GAGGTGGCCC AAATGCAGCG TCCTATCATA CGCGAAGAGG ACTTGGAGCG CCTCAACGCC 
ATCGCCAAGG ACGATAGCTG GACCAAGCAG GACGACATTG ACTTCACGAA GAAACTAGCA 
TTCTCGGACG ATGAGTCCAC TCCGGAAGAG CACCAAGGCC AGGGCAAGCA GCACAGCTCC 
GCCCAACTGC AGGCGCAAGG ATCCTCCTCC TCAACCGATC AACGAAAGCT GTCCGCTTCC 
ACAACCAGTT GGCAGCGCGG CAAGGAGAGC ACCGATCGCG ACCAGCCATC GGAAGGCATT 
CAGGATCAGC AGCAGCAGCT TCAGCAGCAA CAGCAGCAGC AAGAGGTTCG CCAACAGAAT 
GGCCACCGCG GTAGCTCTGG CAACGTTGGA GCACCAGTGG CCGGTGGAAT TGCCTTGGAT 
GCCAGCGTGT ACGAGCGAGT AAAGCAGCGC AAGGAGGAGG AAGAGCGTCG CGAGATGGAA 
CGCAAGCAGG CGGCTGCTAA GAAGTTGCAA GAACTGGAAA TGAAGATGAA TAGCAAAAAG 
GCAGCGGCTG CGGCTCTGGC TGGAGCCGAG GGCGGCGTGT CCTCTGCCTC AGTCCCGTTG 
AGCAATGAGG TGCCACCCAC GTCCGCACCG CTAGGCAATG CCAGCGAAGA TGAGGGCGGA 
GGCCAAGGCG GCGGCGGAGG GTTGAATCGT CGACCCCGGG GAAGCATCTC TAGCTTGGGA 
AGCGGCGTAA CACCCACGGG AGGAGCTGGC GGCGGCGGCG GAGGAGTTTC GTCATCCTCG 
TCAGCAGGCG GCGACTATGG TCAAAAGAAC TTCCTTACCC ATTTCCAATC AAATTTACCA 
CCACGTTTCC AGCGCCAGCA ACAGCAACAA CAGCAGCAGC TGCCGCGCTT GGAGAAGAGC 
GCCTCGGCAA GCAGTGCATT CGATAGCAAT TCCCGCTACC TCCAGAAGGG AGGAGTAAGC 
GGAGGAGGCG GTGGTGTAAG TGGAGGAGCG CAAGCCAATG CTGGCCAGGG TCGCAGCATC 
TCCGGCGGTT ATGTGCAGCG TGGTGCAAGT GTCGGAGGAA GCAGCGGATA CGGACGAAAT 
CGTCACGACA GCAGCAGTGC TCGGGAGGAT CAGGATCTGG ATCAGCAGCG TTTCACCCGT 
GGCCAGGACT ATCGGCAGGC ACAAGCTCTG CCGCGCAGCA TATCTGAGAA CTCGCACCGT 
AAGACCAGCG TCTCATCGGC TGGCGACGAT GTGCTGGGTG GCTCGTGCTC CTCATGGGCG 
GAGCAAACGG ATGCCGAGCA GAAGGTGCAC CATCGGCACC GCGAGGAGAG CTTCTCCTCG 
TCGCACAGCC ATGACTCGGC AGTGCAAATA AAGATCCTGC AACGCCCGGC CCGACAGCCG 
AGCCTCAGTG AGGAGTCCTC GACCCAGCAG AAACAGCTGC CCGCCTCGCC GCAGCAGCAA 
AAGACGCTGA CGACAGATCC CATGCCTACC CAGATTCTGC GACGCAGCGT GGATATCGAT 
AAGTCCGGTG ATGAGCGCGC GGAGGACAAG TCTGCATTGG AATCTGATGA CAAGGCGAAG 
TCGGCTGGCA AGGTGGAGGA GGACAAGAGC GGCAATAAGC GGGTAAGTCC CAGGAGCGGA 
GCTGCTTGCG GAGGTCGAGG TGGCCGTGGT TATGCCGGCA GCTCTGGTGG ATCATCCGTC 
GGATCGGGCA GTAGCTACCG CGGCCAGAGA AATGCCAGCG ATTGGGGAAG CAGTCGTGGA 
AGCGGCGGCG CCGGCGGCAG GCGCTACTAC GGCAGCGGCG GTGAGCAATC GGAGCACTCC 
GAGGATGTGG ACGAGGAGTG TTACAGCAGT GGAGGAGGCG GAGCCGCTCG TCGCAGCCCA 
AAGGAATCGG GCAACGGTGC GCCCAATTCC GGTGCGTCGG CAAACAAGGC AGGATTCTCG 
CCACGCGGCG AACCATCTAG ACGTGGTCGA GGCGGCCTCT CTAGCGGAGG AGGCGCCTCT 
GCTTCTTGTT ACCGTCGACA GCCCGTGGGC ACTGGATCCG CTGGTGGATC TGGTTCGGGC 
GGATCGGGCC GCAGCTATGG CAGACTTGGC TACGAGGAAT ATGGCAAGCA ACAGCGAACT 
TCCGAGTCGG AGACGGACCT AGACAAGACC AAGCAGAACC AGCTGGCACT CAGCGCCGGC 
CTGCACAAAC CAAGCAAGGA TGACAAGGAT TTGCCTGCGC TTGGGGGCGA GGAGAAGGAC 
AAGACTGCCG CCCTGGCGCC TGCCCATAAC ACTGCCCTGC TAGCAAAGGA TGAATTACAG 
AAAGATGCAG CGGGTCGCCT GCCGTTGCCT CCCGGCCTCG CAGCACCTGG CTCTGCAGTG 
GGATCCGTTA CTGCCACATC CTCATCGGTC GTAGTTCCCG GTGCAGCGGA CAAGAAGAAG 
ATTGAATCCT GCGCCGTTGT CGGCGGTGAG AAGGTGACGG GTAGCAAGTT GCCCGCCATT 
GGGGAAAAGA CTTCGCTCGG CGCCGTTGGC TCGGGTCCAT CAACCAGTCT TCTACTGGAC 
GCCGGCGCAC CCGTCAACAC GATTATATTT GAGAACTCCA CGTACAAGCA GCAGGCAGCT 
GCCGCTTCCG TGGTAACCAA ACCCGGAACG CTCTCGGTGT CAAGCAGTGG CGCCACCATG 
ACCGTGGACA GCCTGTCCAG CGCACTCTCC CAAATGAGTT TCGGCGGCAA GGCGACGGCG 
GCGGGCTCTG GCGATGATTC GCAGGATATG AAACTAGGAT TTAGCTTCGG TGACGATCCC 
ACCACACCCC TCAAGGTTGT GGATAACATC GATAAAACGG CCAGTCAGCA GCAGCAACAG 
CAGCAGCAAC AACACTCTCA ACAGCAACAA CAACAGCAGC AGCAGCAAAA CAATTCGACG 
GCGGATCTTA ACATGAAGAT AGCCAGCGTA AAGAAGGTTT GGGAGTCAGC CACGCCCATG 
TCCGACGGCC CATCGCCAGC ACAGGTGCAG CAGCAACAGC AACAGCAGCC CCAACAGCAA 
CAACAGTCCC AGGTGCAGGT GGCGGCGCAG CAGCAACAAC AGCAGGTGGT GGGACCGCAG 
CAGGCAGGTG ACGACGGCAG TGGTCACATG AGCGCCGCCT CCTTTGTGGC AGCCGTGGCC 
GCCTCACAGC ATCAGCAGCA ACACCAGCAA ATGCCGCACT ACGCGGTGTC GGCGGTGCAC 
ATGCAGAGCC ACCACCACCA ACAGCAGCAG CAACAGCAGC AGGCAGCGGT GGCGGCAGCG 
CAGGCGCAGC ACCACCAGCA GCAACAGGCA CAGGCGCACC ACCAGCACCA TGCACTTTCC 
TCGCCGGGCC CGGTGCCCGG TAGTCATGGC CATGGCTACA GTCATGGATC GCCGTTCGAC 
GCTGGATCGT TGGAGCAACA GTTCCAGCAA GAGGATTATC CCAGTCCACA GCAGCAACAG 
CAGGCGCACC AGAAGACCAA GCAGTTGCAG CAGCAGCAGC ATCTGGGCAT GTCACCGCCG 
CCCAGCCAGC AGCAACAGCA GCAGCACTCG CAACAGCAGC AGCAAGCACA GCACCAGCAA 
CAACAGCAGC AGGCCCATCA GCAGCAGCAA CAGCAGCAGC AGCCTTCATT CTACCAGGCG 
TCACCGCAGT TCGGCGTTGG AGGCATTCCC ACTATCCCCA GTCCGCCGGC CGTGGTGTAC 
AACTCGTCGC AGATGCCGCC ACCGTCGCAG GGGGGTAATC TGTACGCTCC ATTCCATTCG 
CTCGATCATT CGAGCCGTTC GCCCGCCTAC TCGGCGGCGG CGGCCGCCCA CAGCTACCCG 
AACCCTTATG CGGCAGCAGC AGCGGCGGCG GCGGCAGCGG CCGCTGGCGG AGGTCCCTTC 
AATGTCAACG TGAACGCATA TGCGATGCAG ACTCCGCACG GCGGCAGCCT ACCGCCCACA 
GCGCCCACAC CGGACATGTA CTCGAACCTC TCTTCGCAGT TTCGATTGGG CGGTGGTCCG 
GGTCCCGGAC CCGGTCCCTT TGGCCAGCCC AGCTCGCAGC AGTTAAGCAA TCCGAGCAAT 
CATGCTGCGG TGGCTATAAT TTCGTCAAGC AGCAACTCCA TGATGTCGTC AGGTGCGGCC 
AAACCGCCGC AGAGCCAGCA GACAATCGGT GCCATTGGCT CCAAGTCGGC AGGTAGCGGT 
GGCGGTCCAG GACCAGGACC TGGTCCCTAT GCCACCACCC AGACGTATAT GAATCTTTAT 
GCGGCTCCAC CGCCGCAACA TCCAGGACAA GGTGGACCAC CGCCGGGTGC CCATCAGCTG 
CAGTCGAACA GTTTCTACTC GAATTCGGCG CCAGGCGGTC CGAGCGGACC ACAGTTCTAC 
GGTGGTCCGC CGCCGCAGGG CAATGGTGGA GCGCAGAGCT ATGGACTGGC CACCGCGGCC 
GGCATGTATG GCGGCCATCA GGGCGGACCG CCCGGCTCCA ATGGGCCGCC GGGACCGCCG 
CAGTCGCAGC ACACGATGGG CACGTTCAAC ACGCCGTTCA TGAACTCGCA GCTGCTGACG 
GCGGCCAGCA TTAATCAGTA CCGCGGCGGA CCAACGCCTG GAGCGGCTTA TCTCAAGGGT 
AACCAGGGCC AGACACACAT GCAAGACTCG ATGGGTCGTC AGCTGAAGAG TCCGCTGAGC 
GCTGACATGA GCCTCGGTCT GGCCAAGCAG GTGCAGTCGC AGCCCAGTCC ACCGCATCAC 
AAGAACTACG GCGCGTGGGA TCTGCAGAAC CAGGTGCTTC AGCAGCAGCA GCAGCAATCT 
CAGCAGCAAC AGCAGCAGCA ACAGCGCCAG GTCGGCGGCG GCAATGGTCC GAGCATGAAC 
GCCTTGGGCG GTCGCGGCAG TGGAGCGGTG GGCAGCGGCA GCGGCAACGG CGGAGGAGGA 
GGAGGAGGAG CTGGTGGGTC CCAAGTGGGC GGCAATGGCG GCGGTAACGG CGTTGGCAGC 
GTGGGCCAAA GTGGCGGCGG CGGCCAGGGA CGCTATCCCA CGCCCATTCA GCGGCCCAAC 
AACTATCCGC AGCATCCGCA ACAACAGCAG CAACAACAGC AGCAACAGCA ACAGCAGAGA 
GAGCAGGCGG CAGCTGCCGC CGTGGCGGCT CAGCGGGCAC AGAATATGCG ACAGGTGACC 
GGTGCCGGCG GAGGCGTGGC CGGAAGTGCA GGTGGCGGTG GTAGTCAAAC ACCAGTGGGA 
CCGGCCGGCG GCAATGGTGG CCCCGGTGGC GCAGGAGGAG GCGGTGGAGC TGGCGGTGCA 
GCAGGATCAG GACCCGGAAA GCCCTACTAT GCGAATAACG CTAGCGGAGC GGCCGTTGCG 
GCCAATCGAG GTGAGTGGCA TGCGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nocte ( NM_132403.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572631.3 Drosophila melanogaster no circadian temperature entrainment (nocte), transcript variant A, mRNA.

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CloneID	OFa01233
Clone ID Related Accession (Same CDS sequence)	NM_001258685.2 , NM_167231.5 , NM_132403.5
Accession Version	NM_132403.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	no circadian temperature entrainment, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132403.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTACAC TGGGGGGAAG TAGGGGAGAG CGAAATGCCA AGCCCAAATT CACAGCGTTA 
GATATAAATC GCATGTACAA GAATAGCCGC GGCGAGTCAA GTGAACCATC TGCACAAAAG 
AATCAAGTGC CACGTAAACA TGGAATGCAA ATCTTGGGCA AAGTGCCGTC CGCTAGGCGA 
CCACCAGCCA ATTTGCCATC CCTGAAGGCC GAGACCCACA CCAGCAGCAA CAACAATCTC 
TTAGTCTCAG TCGAAGGAGC CACATCTGGT GGGGCGGGCG CAGGCGGAGC AGCGGGATCT 
GGCGCGGCAG GAACCAACAC TAGCCACAAC AACACCACTG CCCACAGCGG AAGTTCAGGA 
ACGAGCGGCG GAGCGGGATC AGGCGGCGGA GGAACTGCGT CTTCATCTGC TGGTGGCCAT 
CTATCCCAGC AGCTGACTCA CAGCCAGCAT CAGCACAATA ATAACGTCGC TCTCGGCCCT 
AACGCCGGAA AGTTTGTCAA CAGCAACAAC GGCAGCAGCA ACAACAACGC CGGCAGCTCA 
GGCAGCGGCA ACAAGAACTT CAAATTAATC AACAACTCCG TGGGCAGTTC CGGCGGAGGC 
GGACAAGGAG GGTCGGGCGG CGGCGGATCG TCAGGAGTAG GAGGTGGATC AGGGTCAGGA 
TCGGGCGGAC ACTCAGTCAA CTCACCCAAG ACATGGTCTG CAATTACCAC AGGACACGAG 
CGGGGCTCGG GCGGTCAGAA CTATGGACGC CAGCAACAGC AGGTGGTCCC CCATTATCAG 
AGTCCACAGT TCCAGTACGA ATTTCCCACG CTGGACGGCA CAGTTGGCAG CGGCGCCGGC 
GGAGGATCGG GAGCGGGTGT GGGTTCAGGA TCTGGAGCGG GCAAGAGCCA TTACCAGAAT 
CAGGTGCACC ATGCCGGCCA CCAAAACCAT CACCAGCATC AGCAGCACCA CCAGCAGCAT 
CAACAGCACC ACCACCAGCA GCAACAGCAC CATCGGGACT ACCACCATGG CCACGGGCGC 
CAGTATGGAC ACCACTCGCA TCGCGACTAC AGAGATGGCA GCGACGACGT CGGCGGCGGG 
TCCGCGGATC TGGGCGAGCT CAGCCTTCGG CCACAGAACG ACACGGCCGC CTGGTTGCAG 
CAGCAGGAGA AGGCGGCCAA GGTGGCAGCA GCTGCGGCAG CGGACCAGGG CCTCAACCTA 
CAGGGCCCGG GCCACCATCA GATGGGCGGT AATGGCGGAG CAGGAGGAGG GTCGGGCGGA 
GCTGTGCCCC TGCCCATACT ATCGCTGATG CCCTCGTTCA TGCGTAGCGG AGCTCCGATG 
CCCGCCAGTG GAGCGACAGC GGCCAGCATC TTGGCCGGCG GATCGTTGGA CACTTCTGCG 
TTGCCCAACG CTCCCTACCA GAACGGAATC CTGGGTGCTC GGTCTGCCTC GCCAGCTGTG 
GTGGGCAGTG GAAGCTTCAG GGCGGCCACT GCCATTCCCA AGCGACCACC GACCTCCTCG 
TCGCCCCCAC AATCTGGCCT AGGCATTGGA GGATCACCCA CTCCCCCACA ATCCCAGCAG 
CAGGCCAACG GAGGCGCTGG CGGAGCCGGG CGCAAGGATA AGGACTACGT GGTGGAGCCG 
GAGGTGGCCC AAATGCAGCG TCCTATCATA CGCGAAGAGG ACTTGGAGCG CCTCAACGCC 
ATCGCCAAGG ACGATAGCTG GACCAAGCAG GACGACATTG ACTTCACGAA GAAACTAGCA 
TTCTCGGACG ATGAGTCCAC TCCGGAAGAG CACCAAGGCC AGGGCAAGCA GCACAGCTCC 
GCCCAACTGC AGGCGCAAGG ATCCTCCTCC TCAACCGATC AACGAAAGCT GTCCGCTTCC 
ACAACCAGTT GGCAGCGCGG CAAGGAGAGC ACCGATCGCG ACCAGCCATC GGAAGGCATT 
CAGGATCAGC AGCAGCAGCT TCAGCAGCAA CAGCAGCAGC AAGAGGTTCG CCAACAGAAT 
GGCCACCGCG GTAGCTCTGG CAACGTTGGA GCACCAGTGG CCGGTGGAAT TGCCTTGGAT 
GCCAGCGTGT ACGAGCGAGT AAAGCAGCGC AAGGAGGAGG AAGAGCGTCG CGAGATGGAA 
CGCAAGCAGG CGGCTGCTAA GAAGTTGCAA GAACTGGAAA TGAAGATGAA TAGCAAAAAG 
GCAGCGGCTG CGGCTCTGGC TGGAGCCGAG GGCGGCGTGT CCTCTGCCTC AGTCCCGTTG 
AGCAATGAGG TGCCACCCAC GTCCGCACCG CTAGGCAATG CCAGCGAAGA TGAGGGCGGA 
GGCCAAGGCG GCGGCGGAGG GTTGAATCGT CGACCCCGGG GAAGCATCTC TAGCTTGGGA 
AGCGGCGTAA CACCCACGGG AGGAGCTGGC GGCGGCGGCG GAGGAGTTTC GTCATCCTCG 
TCAGCAGGCG GCGACTATGG TCAAAAGAAC TTCCTTACCC ATTTCCAATC AAATTTACCA 
CCACGTTTCC AGCGCCAGCA ACAGCAACAA CAGCAGCAGC TGCCGCGCTT GGAGAAGAGC 
GCCTCGGCAA GCAGTGCATT CGATAGCAAT TCCCGCTACC TCCAGAAGGG AGGAGTAAGC 
GGAGGAGGCG GTGGTGTAAG TGGAGGAGCG CAAGCCAATG CTGGCCAGGG TCGCAGCATC 
TCCGGCGGTT ATGTGCAGCG TGGTGCAAGT GTCGGAGGAA GCAGCGGATA CGGACGAAAT 
CGTCACGACA GCAGCAGTGC TCGGGAGGAT CAGGATCTGG ATCAGCAGCG TTTCACCCGT 
GGCCAGGACT ATCGGCAGGC ACAAGCTCTG CCGCGCAGCA TATCTGAGAA CTCGCACCGT 
AAGACCAGCG TCTCATCGGC TGGCGACGAT GTGCTGGGTG GCTCGTGCTC CTCATGGGCG 
GAGCAAACGG ATGCCGAGCA GAAGGTGCAC CATCGGCACC GCGAGGAGAG CTTCTCCTCG 
TCGCACAGCC ATGACTCGGC AGTGCAAATA AAGATCCTGC AACGCCCGGC CCGACAGCCG 
AGCCTCAGTG AGGAGTCCTC GACCCAGCAG AAACAGCTGC CCGCCTCGCC GCAGCAGCAA 
AAGACGCTGA CGACAGATCC CATGCCTACC CAGATTCTGC GACGCAGCGT GGATATCGAT 
AAGTCCGGTG ATGAGCGCGC GGAGGACAAG TCTGCATTGG AATCTGATGA CAAGGCGAAG 
TCGGCTGGCA AGGTGGAGGA GGACAAGAGC GGCAATAAGC GGGTAAGTCC CAGGAGCGGA 
GCTGCTTGCG GAGGTCGAGG TGGCCGTGGT TATGCCGGCA GCTCTGGTGG ATCATCCGTC 
GGATCGGGCA GTAGCTACCG CGGCCAGAGA AATGCCAGCG ATTGGGGAAG CAGTCGTGGA 
AGCGGCGGCG CCGGCGGCAG GCGCTACTAC GGCAGCGGCG GTGAGCAATC GGAGCACTCC 
GAGGATGTGG ACGAGGAGTG TTACAGCAGT GGAGGAGGCG GAGCCGCTCG TCGCAGCCCA 
AAGGAATCGG GCAACGGTGC GCCCAATTCC GGTGCGTCGG CAAACAAGGC AGGATTCTCG 
CCACGCGGCG AACCATCTAG ACGTGGTCGA GGCGGCCTCT CTAGCGGAGG AGGCGCCTCT 
GCTTCTTGTT ACCGTCGACA GCCCGTGGGC ACTGGATCCG CTGGTGGATC TGGTTCGGGC 
GGATCGGGCC GCAGCTATGG CAGACTTGGC TACGAGGAAT ATGGCAAGCA ACAGCGAACT 
TCCGAGTCGG AGACGGACCT AGACAAGACC AAGCAGAACC AGCTGGCACT CAGCGCCGGC 
CTGCACAAAC CAAGCAAGGA TGACAAGGAT TTGCCTGCGC TTGGGGGCGA GGAGAAGGAC 
AAGACTGCCG CCCTGGCGCC TGCCCATAAC ACTGCCCTGC TAGCAAAGGA TGAATTACAG 
AAAGATGCAG CGGGTCGCCT GCCGTTGCCT CCCGGCCTCG CAGCACCTGG CTCTGCAGTG 
GGATCCGTTA CTGCCACATC CTCATCGGTC GTAGTTCCCG GTGCAGCGGA CAAGAAGAAG 
ATTGAATCCT GCGCCGTTGT CGGCGGTGAG AAGGTGACGG GTAGCAAGTT GCCCGCCATT 
GGGGAAAAGA CTTCGCTCGG CGCCGTTGGC TCGGGTCCAT CAACCAGTCT TCTACTGGAC 
GCCGGCGCAC CCGTCAACAC GATTATATTT GAGAACTCCA CGTACAAGCA GCAGGCAGCT 
GCCGCTTCCG TGGTAACCAA ACCCGGAACG CTCTCGGTGT CAAGCAGTGG CGCCACCATG 
ACCGTGGACA GCCTGTCCAG CGCACTCTCC CAAATGAGTT TCGGCGGCAA GGCGACGGCG 
GCGGGCTCTG GCGATGATTC GCAGGATATG AAACTAGGAT TTAGCTTCGG TGACGATCCC 
ACCACACCCC TCAAGGTTGT GGATAACATC GATAAAACGG CCAGTCAGCA GCAGCAACAG 
CAGCAGCAAC AACACTCTCA ACAGCAACAA CAACAGCAGC AGCAGCAAAA CAATTCGACG 
GCGGATCTTA ACATGAAGAT AGCCAGCGTA AAGAAGGTTT GGGAGTCAGC CACGCCCATG 
TCCGACGGCC CATCGCCAGC ACAGGTGCAG CAGCAACAGC AACAGCAGCC CCAACAGCAA 
CAACAGTCCC AGGTGCAGGT GGCGGCGCAG CAGCAACAAC AGCAGGTGGT GGGACCGCAG 
CAGGCAGGTG ACGACGGCAG TGGTCACATG AGCGCCGCCT CCTTTGTGGC AGCCGTGGCC 
GCCTCACAGC ATCAGCAGCA ACACCAGCAA ATGCCGCACT ACGCGGTGTC GGCGGTGCAC 
ATGCAGAGCC ACCACCACCA ACAGCAGCAG CAACAGCAGC AGGCAGCGGT GGCGGCAGCG 
CAGGCGCAGC ACCACCAGCA GCAACAGGCA CAGGCGCACC ACCAGCACCA TGCACTTTCC 
TCGCCGGGCC CGGTGCCCGG TAGTCATGGC CATGGCTACA GTCATGGATC GCCGTTCGAC 
GCTGGATCGT TGGAGCAACA GTTCCAGCAA GAGGATTATC CCAGTCCACA GCAGCAACAG 
CAGGCGCACC AGAAGACCAA GCAGTTGCAG CAGCAGCAGC ATCTGGGCAT GTCACCGCCG 
CCCAGCCAGC AGCAACAGCA GCAGCACTCG CAACAGCAGC AGCAAGCACA GCACCAGCAA 
CAACAGCAGC AGGCCCATCA GCAGCAGCAA CAGCAGCAGC AGCCTTCATT CTACCAGGCG 
TCACCGCAGT TCGGCGTTGG AGGCATTCCC ACTATCCCCA GTCCGCCGGC CGTGGTGTAC 
AACTCGTCGC AGATGCCGCC ACCGTCGCAG GGGGGTAATC TGTACGCTCC ATTCCATTCG 
CTCGATCATT CGAGCCGTTC GCCCGCCTAC TCGGCGGCGG CGGCCGCCCA CAGCTACCCG 
AACCCTTATG CGGCAGCAGC AGCGGCGGCG GCGGCAGCGG CCGCTGGCGG AGGTCCCTTC 
AATGTCAACG TGAACGCATA TGCGATGCAG ACTCCGCACG GCGGCAGCCT ACCGCCCACA 
GCGCCCACAC CGGACATGTA CTCGAACCTC TCTTCGCAGT TTCGATTGGG CGGTGGTCCG 
GGTCCCGGAC CCGGTCCCTT TGGCCAGCCC AGCTCGCAGC AGTTAAGCAA TCCGAGCAAT 
CATGCTGCGG TGGCTATAAT TTCGTCAAGC AGCAACTCCA TGATGTCGTC AGGTGCGGCC 
AAACCGCCGC AGAGCCAGCA GACAATCGGT GCCATTGGCT CCAAGTCGGC AGGTAGCGGT 
GGCGGTCCAG GACCAGGACC TGGTCCCTAT GCCACCACCC AGACGTATAT GAATCTTTAT 
GCGGCTCCAC CGCCGCAACA TCCAGGACAA GGTGGACCAC CGCCGGGTGC CCATCAGCTG 
CAGTCGAACA GTTTCTACTC GAATTCGGCG CCAGGCGGTC CGAGCGGACC ACAGTTCTAC 
GGTGGTCCGC CGCCGCAGGG CAATGGTGGA GCGCAGAGCT ATGGACTGGC CACCGCGGCC 
GGCATGTATG GCGGCCATCA GGGCGGACCG CCCGGCTCCA ATGGGCCGCC GGGACCGCCG 
CAGTCGCAGC ACACGATGGG CACGTTCAAC ACGCCGTTCA TGAACTCGCA GCTGCTGACG 
GCGGCCAGCA TTAATCAGTA CCGCGGCGGA CCAACGCCTG GAGCGGCTTA TCTCAAGGGT 
AACCAGGGCC AGACACACAT GCAAGACTCG ATGGGTCGTC AGCTGAAGAG TCCGCTGAGC 
GCTGACATGA GCCTCGGTCT GGCCAAGCAG GTGCAGTCGC AGCCCAGTCC ACCGCATCAC 
AAGAACTACG GCGCGTGGGA TCTGCAGAAC CAGGTGCTTC AGCAGCAGCA GCAGCAATCT 
CAGCAGCAAC AGCAGCAGCA ACAGCGCCAG GTCGGCGGCG GCAATGGTCC GAGCATGAAC 
GCCTTGGGCG GTCGCGGCAG TGGAGCGGTG GGCAGCGGCA GCGGCAACGG CGGAGGAGGA 
GGAGGAGGAG CTGGTGGGTC CCAAGTGGGC GGCAATGGCG GCGGTAACGG CGTTGGCAGC 
GTGGGCCAAA GTGGCGGCGG CGGCCAGGGA CGCTATCCCA CGCCCATTCA GCGGCCCAAC 
AACTATCCGC AGCATCCGCA ACAACAGCAG CAACAACAGC AGCAACAGCA ACAGCAGAGA 
GAGCAGGCGG CAGCTGCCGC CGTGGCGGCT CAGCGGGCAC AGAATATGCG ACAGGTGACC 
GGTGCCGGCG GAGGCGTGGC CGGAAGTGCA GGTGGCGGTG GTAGTCAAAC ACCAGTGGGA 
CCGGCCGGCG GCAATGGTGG CCCCGGTGGC GCAGGAGGAG GCGGTGGAGC TGGCGGTGCA 
GCAGGATCAG GACCCGGAAA GCCCTACTAT GCGAATAACG CTAGCGGAGC GGCCGTTGCG 
GCCAATCGAG GTGAGTGGCA TGCGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572631.3
CDS	594..7523
Translation	MSTLGGSRGERNAKPKFTALDINRMYKNSRGESSEPSAQKNQVPRKHGMQILGKVPSARRPPANLPSLKAETHTSSNNNLLVSVEGATSGGAGAGGAAGSGAAGTNTSHNNTTAHSGSSGTSGGAGSGGGGTASSSAGGHLSQQLTHSQHQHNNNVALGPNAGKFVNSNNGSSNNNAGSSGSGNKNFKLINNSVGSSGGGGQGGSGGGGSSGVGGGSGSGSGGHSVNSPKTWSAITTGHERGSGGQNYGRQQQQVVPHYQSPQFQYEFPTLDGTVGSGAGGGSGAGVGSGSGAGKSHYQNQVHHAGHQNHHQHQQHHQQHQQHHHQQQQHHRDYHHGHGRQYGHHSHRDYRDGSDDVGGGSADLGELSLRPQNDTAAWLQQQEKAAKVAAAAAADQGLNLQGPGHHQMGGNGGAGGGSGGAVPLPILSLMPSFMRSGAPMPASGATAASILAGGSLDTSALPNAPYQNGILGARSASPAVVGSGSFRAATAIPKRPPTSSSPPQSGLGIGGSPTPPQSQQQANGGAGGAGRKDKDYVVEPEVAQMQRPIIREEDLERLNAIAKDDSWTKQDDIDFTKKLAFSDDESTPEEHQGQGKQHSSAQLQAQGSSSSTDQRKLSASTTSWQRGKESTDRDQPSEGIQDQQQQLQQQQQQQEVRQQNGHRGSSGNVGAPVAGGIALDASVYERVKQRKEEEERREMERKQAAAKKLQELEMKMNSKKAAAAALAGAEGGVSSASVPLSNEVPPTSAPLGNASEDEGGGQGGGGGLNRRPRGSISSLGSGVTPTGGAGGGGGGVSSSSSAGGDYGQKNFLTHFQSNLPPRFQRQQQQQQQQLPRLEKSASASSAFDSNSRYLQKGGVSGGGGGVSGGAQANAGQGRSISGGYVQRGASVGGSSGYGRNRHDSSSAREDQDLDQQRFTRGQDYRQAQALPRSISENSHRKTSVSSAGDDVLGGSCSSWAEQTDAEQKVHHRHREESFSSSHSHDSAVQIKILQRPARQPSLSEESSTQQKQLPASPQQQKTLTTDPMPTQILRRSVDIDKSGDERAEDKSALESDDKAKSAGKVEEDKSGNKRVSPRSGAACGGRGGRGYAGSSGGSSVGSGSSYRGQRNASDWGSSRGSGGAGGRRYYGSGGEQSEHSEDVDEECYSSGGGGAARRSPKESGNGAPNSGASANKAGFSPRGEPSRRGRGGLSSGGGASASCYRRQPVGTGSAGGSGSGGSGRSYGRLGYEEYGKQQRTSESETDLDKTKQNQLALSAGLHKPSKDDKDLPALGGEEKDKTAALAPAHNTALLAKDELQKDAAGRLPLPPGLAAPGSAVGSVTATSSSVVVPGAADKKKIESCAVVGGEKVTGSKLPAIGEKTSLGAVGSGPSTSLLLDAGAPVNTIIFENSTYKQQAAAASVVTKPGTLSVSSSGATMTVDSLSSALSQMSFGGKATAAGSGDDSQDMKLGFSFGDDPTTPLKVVDNIDKTASQQQQQQQQQHSQQQQQQQQQQNNSTADLNMKIASVKKVWESATPMSDGPSPAQVQQQQQQQPQQQQQSQVQVAAQQQQQQVVGPQQAGDDGSGHMSAASFVAAVAASQHQQQHQQMPHYAVSAVHMQSHHHQQQQQQQQAAVAAAQAQHHQQQQAQAHHQHHALSSPGPVPGSHGHGYSHGSPFDAGSLEQQFQQEDYPSPQQQQQAHQKTKQLQQQQHLGMSPPPSQQQQQQHSQQQQQAQHQQQQQQAHQQQQQQQQPSFYQASPQFGVGGIPTIPSPPAVVYNSSQMPPPSQGGNLYAPFHSLDHSSRSPAYSAAAAAHSYPNPYAAAAAAAAAAAAGGGPFNVNVNAYAMQTPHGGSLPPTAPTPDMYSNLSSQFRLGGGPGPGPGPFGQPSSQQLSNPSNHAAVAIISSSSNSMMSSGAAKPPQSQQTIGAIGSKSAGSGGGPGPGPGPYATTQTYMNLYAAPPPQHPGQGGPPPGAHQLQSNSFYSNSAPGGPSGPQFYGGPPPQGNGGAQSYGLATAAGMYGGHQGGPPGSNGPPGPPQSQHTMGTFNTPFMNSQLLTAASINQYRGGPTPGAAYLKGNQGQTHMQDSMGRQLKSPLSADMSLGLAKQVQSQPSPPHHKNYGAWDLQNQVLQQQQQQSQQQQQQQQRQVGGGNGPSMNALGGRGSGAVGSGSGNGGGGGGGAGGSQVGGNGGGNGVGSVGQSGGGGQGRYPTPIQRPNNYPQHPQQQQQQQQQQQQQREQAAAAAVAAQRAQNMRQVTGAGGGVAGSAGGGGSQTPVGPAGGNGGPGGAGGGGGAGGAAGSGPGKPYYANNASGAAVAANRGEWHAN

Target ORF information:

RefSeq Version	NM_132403.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster no circadian temperature entrainment (nocte), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132403.5

ORF Insert Sequence:

ATGAGTACAC TGGGGGGAAG TAGGGGAGAG CGAAATGCCA AGCCCAAATT CACAGCGTTA 
GATATAAATC GCATGTACAA GAATAGCCGC GGCGAGTCAA GTGAACCATC TGCACAAAAG 
AATCAAGTGC CACGTAAACA TGGAATGCAA ATCTTGGGCA AAGTGCCGTC CGCTAGGCGA 
CCACCAGCCA ATTTGCCATC CCTGAAGGCC GAGACCCACA CCAGCAGCAA CAACAATCTC 
TTAGTCTCAG TCGAAGGAGC CACATCTGGT GGGGCGGGCG CAGGCGGAGC AGCGGGATCT 
GGCGCGGCAG GAACCAACAC TAGCCACAAC AACACCACTG CCCACAGCGG AAGTTCAGGA 
ACGAGCGGCG GAGCGGGATC AGGCGGCGGA GGAACTGCGT CTTCATCTGC TGGTGGCCAT 
CTATCCCAGC AGCTGACTCA CAGCCAGCAT CAGCACAATA ATAACGTCGC TCTCGGCCCT 
AACGCCGGAA AGTTTGTCAA CAGCAACAAC GGCAGCAGCA ACAACAACGC CGGCAGCTCA 
GGCAGCGGCA ACAAGAACTT CAAATTAATC AACAACTCCG TGGGCAGTTC CGGCGGAGGC 
GGACAAGGAG GGTCGGGCGG CGGCGGATCG TCAGGAGTAG GAGGTGGATC AGGGTCAGGA 
TCGGGCGGAC ACTCAGTCAA CTCACCCAAG ACATGGTCTG CAATTACCAC AGGACACGAG 
CGGGGCTCGG GCGGTCAGAA CTATGGACGC CAGCAACAGC AGGTGGTCCC CCATTATCAG 
AGTCCACAGT TCCAGTACGA ATTTCCCACG CTGGACGGCA CAGTTGGCAG CGGCGCCGGC 
GGAGGATCGG GAGCGGGTGT GGGTTCAGGA TCTGGAGCGG GCAAGAGCCA TTACCAGAAT 
CAGGTGCACC ATGCCGGCCA CCAAAACCAT CACCAGCATC AGCAGCACCA CCAGCAGCAT 
CAACAGCACC ACCACCAGCA GCAACAGCAC CATCGGGACT ACCACCATGG CCACGGGCGC 
CAGTATGGAC ACCACTCGCA TCGCGACTAC AGAGATGGCA GCGACGACGT CGGCGGCGGG 
TCCGCGGATC TGGGCGAGCT CAGCCTTCGG CCACAGAACG ACACGGCCGC CTGGTTGCAG 
CAGCAGGAGA AGGCGGCCAA GGTGGCAGCA GCTGCGGCAG CGGACCAGGG CCTCAACCTA 
CAGGGCCCGG GCCACCATCA GATGGGCGGT AATGGCGGAG CAGGAGGAGG GTCGGGCGGA 
GCTGTGCCCC TGCCCATACT ATCGCTGATG CCCTCGTTCA TGCGTAGCGG AGCTCCGATG 
CCCGCCAGTG GAGCGACAGC GGCCAGCATC TTGGCCGGCG GATCGTTGGA CACTTCTGCG 
TTGCCCAACG CTCCCTACCA GAACGGAATC CTGGGTGCTC GGTCTGCCTC GCCAGCTGTG 
GTGGGCAGTG GAAGCTTCAG GGCGGCCACT GCCATTCCCA AGCGACCACC GACCTCCTCG 
TCGCCCCCAC AATCTGGCCT AGGCATTGGA GGATCACCCA CTCCCCCACA ATCCCAGCAG 
CAGGCCAACG GAGGCGCTGG CGGAGCCGGG CGCAAGGATA AGGACTACGT GGTGGAGCCG 
GAGGTGGCCC AAATGCAGCG TCCTATCATA CGCGAAGAGG ACTTGGAGCG CCTCAACGCC 
ATCGCCAAGG ACGATAGCTG GACCAAGCAG GACGACATTG ACTTCACGAA GAAACTAGCA 
TTCTCGGACG ATGAGTCCAC TCCGGAAGAG CACCAAGGCC AGGGCAAGCA GCACAGCTCC 
GCCCAACTGC AGGCGCAAGG ATCCTCCTCC TCAACCGATC AACGAAAGCT GTCCGCTTCC 
ACAACCAGTT GGCAGCGCGG CAAGGAGAGC ACCGATCGCG ACCAGCCATC GGAAGGCATT 
CAGGATCAGC AGCAGCAGCT TCAGCAGCAA CAGCAGCAGC AAGAGGTTCG CCAACAGAAT 
GGCCACCGCG GTAGCTCTGG CAACGTTGGA GCACCAGTGG CCGGTGGAAT TGCCTTGGAT 
GCCAGCGTGT ACGAGCGAGT AAAGCAGCGC AAGGAGGAGG AAGAGCGTCG CGAGATGGAA 
CGCAAGCAGG CGGCTGCTAA GAAGTTGCAA GAACTGGAAA TGAAGATGAA TAGCAAAAAG 
GCAGCGGCTG CGGCTCTGGC TGGAGCCGAG GGCGGCGTGT CCTCTGCCTC AGTCCCGTTG 
AGCAATGAGG TGCCACCCAC GTCCGCACCG CTAGGCAATG CCAGCGAAGA TGAGGGCGGA 
GGCCAAGGCG GCGGCGGAGG GTTGAATCGT CGACCCCGGG GAAGCATCTC TAGCTTGGGA 
AGCGGCGTAA CACCCACGGG AGGAGCTGGC GGCGGCGGCG GAGGAGTTTC GTCATCCTCG 
TCAGCAGGCG GCGACTATGG TCAAAAGAAC TTCCTTACCC ATTTCCAATC AAATTTACCA 
CCACGTTTCC AGCGCCAGCA ACAGCAACAA CAGCAGCAGC TGCCGCGCTT GGAGAAGAGC 
GCCTCGGCAA GCAGTGCATT CGATAGCAAT TCCCGCTACC TCCAGAAGGG AGGAGTAAGC 
GGAGGAGGCG GTGGTGTAAG TGGAGGAGCG CAAGCCAATG CTGGCCAGGG TCGCAGCATC 
TCCGGCGGTT ATGTGCAGCG TGGTGCAAGT GTCGGAGGAA GCAGCGGATA CGGACGAAAT 
CGTCACGACA GCAGCAGTGC TCGGGAGGAT CAGGATCTGG ATCAGCAGCG TTTCACCCGT 
GGCCAGGACT ATCGGCAGGC ACAAGCTCTG CCGCGCAGCA TATCTGAGAA CTCGCACCGT 
AAGACCAGCG TCTCATCGGC TGGCGACGAT GTGCTGGGTG GCTCGTGCTC CTCATGGGCG 
GAGCAAACGG ATGCCGAGCA GAAGGTGCAC CATCGGCACC GCGAGGAGAG CTTCTCCTCG 
TCGCACAGCC ATGACTCGGC AGTGCAAATA AAGATCCTGC AACGCCCGGC CCGACAGCCG 
AGCCTCAGTG AGGAGTCCTC GACCCAGCAG AAACAGCTGC CCGCCTCGCC GCAGCAGCAA 
AAGACGCTGA CGACAGATCC CATGCCTACC CAGATTCTGC GACGCAGCGT GGATATCGAT 
AAGTCCGGTG ATGAGCGCGC GGAGGACAAG TCTGCATTGG AATCTGATGA CAAGGCGAAG 
TCGGCTGGCA AGGTGGAGGA GGACAAGAGC GGCAATAAGC GGGTAAGTCC CAGGAGCGGA 
GCTGCTTGCG GAGGTCGAGG TGGCCGTGGT TATGCCGGCA GCTCTGGTGG ATCATCCGTC 
GGATCGGGCA GTAGCTACCG CGGCCAGAGA AATGCCAGCG ATTGGGGAAG CAGTCGTGGA 
AGCGGCGGCG CCGGCGGCAG GCGCTACTAC GGCAGCGGCG GTGAGCAATC GGAGCACTCC 
GAGGATGTGG ACGAGGAGTG TTACAGCAGT GGAGGAGGCG GAGCCGCTCG TCGCAGCCCA 
AAGGAATCGG GCAACGGTGC GCCCAATTCC GGTGCGTCGG CAAACAAGGC AGGATTCTCG 
CCACGCGGCG AACCATCTAG ACGTGGTCGA GGCGGCCTCT CTAGCGGAGG AGGCGCCTCT 
GCTTCTTGTT ACCGTCGACA GCCCGTGGGC ACTGGATCCG CTGGTGGATC TGGTTCGGGC 
GGATCGGGCC GCAGCTATGG CAGACTTGGC TACGAGGAAT ATGGCAAGCA ACAGCGAACT 
TCCGAGTCGG AGACGGACCT AGACAAGACC AAGCAGAACC AGCTGGCACT CAGCGCCGGC 
CTGCACAAAC CAAGCAAGGA TGACAAGGAT TTGCCTGCGC TTGGGGGCGA GGAGAAGGAC 
AAGACTGCCG CCCTGGCGCC TGCCCATAAC ACTGCCCTGC TAGCAAAGGA TGAATTACAG 
AAAGATGCAG CGGGTCGCCT GCCGTTGCCT CCCGGCCTCG CAGCACCTGG CTCTGCAGTG 
GGATCCGTTA CTGCCACATC CTCATCGGTC GTAGTTCCCG GTGCAGCGGA CAAGAAGAAG 
ATTGAATCCT GCGCCGTTGT CGGCGGTGAG AAGGTGACGG GTAGCAAGTT GCCCGCCATT 
GGGGAAAAGA CTTCGCTCGG CGCCGTTGGC TCGGGTCCAT CAACCAGTCT TCTACTGGAC 
GCCGGCGCAC CCGTCAACAC GATTATATTT GAGAACTCCA CGTACAAGCA GCAGGCAGCT 
GCCGCTTCCG TGGTAACCAA ACCCGGAACG CTCTCGGTGT CAAGCAGTGG CGCCACCATG 
ACCGTGGACA GCCTGTCCAG CGCACTCTCC CAAATGAGTT TCGGCGGCAA GGCGACGGCG 
GCGGGCTCTG GCGATGATTC GCAGGATATG AAACTAGGAT TTAGCTTCGG TGACGATCCC 
ACCACACCCC TCAAGGTTGT GGATAACATC GATAAAACGG CCAGTCAGCA GCAGCAACAG 
CAGCAGCAAC AACACTCTCA ACAGCAACAA CAACAGCAGC AGCAGCAAAA CAATTCGACG 
GCGGATCTTA ACATGAAGAT AGCCAGCGTA AAGAAGGTTT GGGAGTCAGC CACGCCCATG 
TCCGACGGCC CATCGCCAGC ACAGGTGCAG CAGCAACAGC AACAGCAGCC CCAACAGCAA 
CAACAGTCCC AGGTGCAGGT GGCGGCGCAG CAGCAACAAC AGCAGGTGGT GGGACCGCAG 
CAGGCAGGTG ACGACGGCAG TGGTCACATG AGCGCCGCCT CCTTTGTGGC AGCCGTGGCC 
GCCTCACAGC ATCAGCAGCA ACACCAGCAA ATGCCGCACT ACGCGGTGTC GGCGGTGCAC 
ATGCAGAGCC ACCACCACCA ACAGCAGCAG CAACAGCAGC AGGCAGCGGT GGCGGCAGCG 
CAGGCGCAGC ACCACCAGCA GCAACAGGCA CAGGCGCACC ACCAGCACCA TGCACTTTCC 
TCGCCGGGCC CGGTGCCCGG TAGTCATGGC CATGGCTACA GTCATGGATC GCCGTTCGAC 
GCTGGATCGT TGGAGCAACA GTTCCAGCAA GAGGATTATC CCAGTCCACA GCAGCAACAG 
CAGGCGCACC AGAAGACCAA GCAGTTGCAG CAGCAGCAGC ATCTGGGCAT GTCACCGCCG 
CCCAGCCAGC AGCAACAGCA GCAGCACTCG CAACAGCAGC AGCAAGCACA GCACCAGCAA 
CAACAGCAGC AGGCCCATCA GCAGCAGCAA CAGCAGCAGC AGCCTTCATT CTACCAGGCG 
TCACCGCAGT TCGGCGTTGG AGGCATTCCC ACTATCCCCA GTCCGCCGGC CGTGGTGTAC 
AACTCGTCGC AGATGCCGCC ACCGTCGCAG GGGGGTAATC TGTACGCTCC ATTCCATTCG 
CTCGATCATT CGAGCCGTTC GCCCGCCTAC TCGGCGGCGG CGGCCGCCCA CAGCTACCCG 
AACCCTTATG CGGCAGCAGC AGCGGCGGCG GCGGCAGCGG CCGCTGGCGG AGGTCCCTTC 
AATGTCAACG TGAACGCATA TGCGATGCAG ACTCCGCACG GCGGCAGCCT ACCGCCCACA 
GCGCCCACAC CGGACATGTA CTCGAACCTC TCTTCGCAGT TTCGATTGGG CGGTGGTCCG 
GGTCCCGGAC CCGGTCCCTT TGGCCAGCCC AGCTCGCAGC AGTTAAGCAA TCCGAGCAAT 
CATGCTGCGG TGGCTATAAT TTCGTCAAGC AGCAACTCCA TGATGTCGTC AGGTGCGGCC 
AAACCGCCGC AGAGCCAGCA GACAATCGGT GCCATTGGCT CCAAGTCGGC AGGTAGCGGT 
GGCGGTCCAG GACCAGGACC TGGTCCCTAT GCCACCACCC AGACGTATAT GAATCTTTAT 
GCGGCTCCAC CGCCGCAACA TCCAGGACAA GGTGGACCAC CGCCGGGTGC CCATCAGCTG 
CAGTCGAACA GTTTCTACTC GAATTCGGCG CCAGGCGGTC CGAGCGGACC ACAGTTCTAC 
GGTGGTCCGC CGCCGCAGGG CAATGGTGGA GCGCAGAGCT ATGGACTGGC CACCGCGGCC 
GGCATGTATG GCGGCCATCA GGGCGGACCG CCCGGCTCCA ATGGGCCGCC GGGACCGCCG 
CAGTCGCAGC ACACGATGGG CACGTTCAAC ACGCCGTTCA TGAACTCGCA GCTGCTGACG 
GCGGCCAGCA TTAATCAGTA CCGCGGCGGA CCAACGCCTG GAGCGGCTTA TCTCAAGGGT 
AACCAGGGCC AGACACACAT GCAAGACTCG ATGGGTCGTC AGCTGAAGAG TCCGCTGAGC 
GCTGACATGA GCCTCGGTCT GGCCAAGCAG GTGCAGTCGC AGCCCAGTCC ACCGCATCAC 
AAGAACTACG GCGCGTGGGA TCTGCAGAAC CAGGTGCTTC AGCAGCAGCA GCAGCAATCT 
CAGCAGCAAC AGCAGCAGCA ACAGCGCCAG GTCGGCGGCG GCAATGGTCC GAGCATGAAC 
GCCTTGGGCG GTCGCGGCAG TGGAGCGGTG GGCAGCGGCA GCGGCAACGG CGGAGGAGGA 
GGAGGAGGAG CTGGTGGGTC CCAAGTGGGC GGCAATGGCG GCGGTAACGG CGTTGGCAGC 
GTGGGCCAAA GTGGCGGCGG CGGCCAGGGA CGCTATCCCA CGCCCATTCA GCGGCCCAAC 
AACTATCCGC AGCATCCGCA ACAACAGCAG CAACAACAGC AGCAACAGCA ACAGCAGAGA 
GAGCAGGCGG CAGCTGCCGC CGTGGCGGCT CAGCGGGCAC AGAATATGCG ACAGGTGACC 
GGTGCCGGCG GAGGCGTGGC CGGAAGTGCA GGTGGCGGTG GTAGTCAAAC ACCAGTGGGA 
CCGGCCGGCG GCAATGGTGG CCCCGGTGGC GCAGGAGGAG GCGGTGGAGC TGGCGGTGCA 
GCAGGATCAG GACCCGGAAA GCCCTACTAT GCGAATAACG CTAGCGGAGC GGCCGTTGCG 
GCCAATCGAG GTGAGTGGCA TGCGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nocte ( NM_001272480.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259409.1 Drosophila melanogaster no circadian temperature entrainment (nocte), transcript variant D, mRNA.

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CloneID	OFa03412
Clone ID Related Accession (Same CDS sequence)	NM_001272480.1
Accession Version	NM_001272480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	no circadian temperature entrainment, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTACAC TGGGGGGAAG TAGGGGAGAG CGAAATGCCA AGCCCAAATT CACAGCGTTA 
GATATAAATC GCATGTACAA GAATAGCCGC GGCGAGTCAA GTGAACCATC TGCACAAAAG 
AATCAAGTGC CACGTAAACA TGGAATGCAA ATCTTGGGCA AAGTGCCGTC CGCTAGGCGA 
CCACCAGCCA ATTTGCCATC CCTGAAGGCC GAGACCCACA CCAGCAGCAA CAACAATCTC 
TTAGTCTCAG TCGAAGGAGC CACATCTGGT GGGGCGGGCG CAGGCGGAGC AGCGGGATCT 
GGCGCGGCAG GAACCAACAC TAGCCACAAC AACACCACTG CCCACAGCGG AAGTTCAGGA 
ACGAGCGGCG GAGCGGGATC AGGCGGCGGA GGAACTGCGT CTTCATCTGC TGGTGGCCAT 
CTATCCCAGC AGCTGACTCA CAGCCAGCAT CAGCACAATA ATAACGTCGC TCTCGGCCCT 
AACGCCGGAA AGTTTGTCAA CAGCAACAAC GGCAGCAGCA ACAACAACGC CGGCAGCTCA 
GGCAGCGGCA ACAAGAACTT CAAATTAATC AACAACTCCG TGGGCAGTTC CGGCGGAGGC 
GGACAAGGAG GGTCGGGCGG CGGCGGATCG TCAGGAGTAG GAGGTGGATC AGGGTCAGGA 
TCGGGCGGAC ACTCAGTCAA CTCACCCAAG ACATGGTCTG CAATTACCAC AGGACACGAG 
CGGGGCTCGG GCGGTCAGAA CTATGGACGC CAGCAACAGC AGGTGGTCCC CCATTATCAG 
AGTCCACAGT TCCAGTACGA ATTTCCCACG CTGGACGGCA CAGTTGGCAG CGGCGCCGGC 
GGAGGATCGG GAGCGGGTGT GGGTTCAGGA TCTGGAGCGG GCAAGAGCCA TTACCAGAAT 
CAGGTGCACC ATGCCGGCCA CCAAAACCAT CACCAGCATC AGCAGCACCA CCAGCAGCAT 
CAACAGCACC ACCACCAGCA GCAACAGCAC CATCGGGACT ACCACCATGG CCACGGGCGC 
CAGTATGGAC ACCACTCGCA TCGCGACTAC AGAGATGGCA GCGACGACGT CGGCGGCGGG 
TCCGCGGATC TGGGCGAGCT CAGCCTTCGG CCACAGAACG ACACGGCCGC CTGGTTGCAG 
CAGCAGGAGA AGGCGGCCAA GGTGGCAGCA GCTGCGGCAG CGGACCAGGG CCTCAACCTA 
CAGGGCCCGG GCCACCATCA GATGGGCGGT AATGGCGGAG CAGGAGGAGG GTCGGGCGGA 
GCTGTGCCCC TGCCCATACT ATCGCTGATG CCCTCGTTCA TGCGTAGCGG AGCTCCGATG 
CCCGCCAGTG GAGCGACAGC GGCCAGCATC TTGGCCGGCG GATCGTTGGA CACTTCTGCG 
TTGCCCAACG CTCCCTACCA GAACGGAATC CTGGGTGCTC GGTCTGCCTC GCCAGCTGTG 
GTGGGCAGTG GAAGCTTCAG GGCGGCCACT GCCATTCCCA AGCGACCACC GACCTCCTCG 
TCGCCCCCAC AATCTGGCCT AGGCATTGGA GGATCACCCA CTCCCCCACA ATCCCAGCAG 
CAGGCCAACG GAGGCGCTGG CGGAGCCGGG CGCAAGGATA AGGACTACGT GGTGGAGCCG 
GAGGTGGCCC AAATGCAGCG TCCTATCATA CGCGAAGAGG ACTTGGAGCG CCTCAACGCC 
ATCGCCAAGG ACGATAGCTG GACCAAGCAG GACGACATTG ACTTCACGAA GAAACTAGCA 
TTCTCGGACG ATGAGTCCAC TCCGGAAGAG CACCAAGGCC AGGGCAAGCA GCACAGCTCC 
GCCCAACTGC AGGCGCAAGG ATCCTCCTCC TCAACCGATC AACGAAAGCT GTCCGCTTCC 
ACAACCAGTT GGCAGCGCGG CAAGGAGAGC ACCGATCGCG ACCAGCCATC GGAAGGCATT 
CAGGATCAGC AGCAGCAGCT TCAGCAGCAA CAGCAGCAGC AAGAGGTTCG CCAACAGAAT 
GGCCACCGCG GTAGCTCTGG CAACGTTGGA GCACCAGTGG CCGGTGGAAT TGCCTTGGAT 
GCCAGCGTGT ACGAGCGAGT AAAGCAGCGC AAGGAGGAGG AAGAGCGTCG CGAGATGGAA 
CGCAAGCAGG CGGCTGCTAA GAAGTTGCAA GAACTGGAAA TGAAGATGAA TAGCAAAAAG 
GCAGCGGCTG CGGCTCTGGC TGGAGCCGAG GGCGGCGTGT CCTCTGCCTC AGTCCCGTTG 
AGCAATGAGG TGCCACCCAC GTCCGCACCG CTAGGCAATG CCAGCGAAGA TGAGGGCGGA 
GGCCAAGGCG GCGGCGGAGG GTTGAATCGT CGACCCCGGG GAAGCATCTC TAGCTTGGGA 
AGCGGCGTAA CACCCACGGG AGGAGCTGGC GGCGGCGGCG GAGGAGTTTC GTCATCCTCG 
TCAGCAGGCG GCGACTATGG TCAAAAGAAC TTCCTTACCC ATTTCCAATC AAATTTACCA 
CCACGTTTCC AGCGCCAGCA ACAGCAACAA CAGCAGCAGC TGCCGCGCTT GGAGAAGAGC 
GCCTCGGCAA GCAGTGCATT CGATAGCAAT TCCCGCTACC TCCAGAAGGG AGGAGTAAGC 
GGAGGAGGCG GTGGTGTAAG TGGAGGAGCG CAAGCCAATG CTGGCCAGGG TCGCAGCATC 
TCCGGCGGTT ATGTGCAGCG TGGTGCAAGT GTCGGAGGAA GCAGCGGATA CGGACGAAAT 
CGTCACGACA GCAGCAGTGC TCGGGAGGAT CAGGATCTGG ATCAGCAGCG TTTCACCCGT 
GGCCAGGACT ATCGGCAGGC ACAAGCTCTG CCGCGCAGCA TATCTGAGAA CTCGCACCGT 
AAGACCAGCG TCTCATCGGC TGGCGACGAT GTGCTGGGTG GCTCGTGCTC CTCATGGGCG 
GAGCAAACGG ATGCCGAGCA GAAGGTGCAC CATCGGCACC GCGAGGAGAG CTTCTCCTCG 
TCGCACAGCC ATGACTCGGC AGTGCAAATA AAGATCCTGC AACGCCCGGC CCGACAGCCG 
AGCCTCAGTG AGGAGTCCTC GACCCAGCAG AAACAGCTGC CCGCCTCGCC GCAGCAGCAA 
AAGACGCTGA CGACAGATCC CATGCCTACC CAGATTCTGC GACGCAGCGT GGATATCGAT 
AAGTCCGGTG ATGAGCGCGC GGAGGACAAG TCTGCATTGG AATCTGATGA CAAGGCGAAG 
TCGGCTGGCA AGGTGGAGGA GGACAAGAGC GGCAATAAGC GGGTAAGTCC CAGGAGCGGA 
GCTGCTTGCG GAGGTCGAGG TGGCCGTGGT TATGCCGGCA GCTCTGGTGG ATCATCCGTC 
GGATCGGGCA GTAGCTACCG CGGCCAGAGA AATGCCAGCG ATTGGGGAAG CAGTCGTGGA 
AGCGGCGGCG CCGGCGGCAG GCGCTACTAC GGCAGCGGCG GTGAGCAATC GGAGCACTCC 
GAGGATGTGG ACGAGGAGTG TTACAGCAGT GGAGGAGGCG GAGCCGCTCG TCGCAGCCCA 
AAGGAATCGG GCAACGGTGC GCCCAATTCC GGTGCGTCGG CAAACAAGGC AGGATTCTCG 
CCACGCGGCG AACCATCTAG ACGTGGTCGA GGCGGCCTCT CTAGCGGAGG AGGCGCCTCT 
GCTTCTTGTT ACCGTCGACA GCCCGTGGGC ACTGGATCCG CTGGTGGATC TGGTTCGGGC 
GGATCGGGCC GCAGCTATGG CAGACTTGGC TACGAGGAAT ATGGCAAGCA ACAGCGAACT 
TCCGAGTCGG AGACGGACCT AGACAAGACC AAGCAGAACC AGCTGGCACT CAGCGCCGGC 
CTGCACAAAC CAAGCAAGGA TGACAAGGAT TTGCCTGCGC TTGGGGGCGA GGAGAAGGAC 
AAGACTGCCG CCCTGGCGCC TGCCCATAAC ACTGCCCTGC TAGCAAAGGA TGAATTACAG 
AAAGATGCAG CGGGTCGCCT GCCGTTGCCT CCCGGCCTCG CAGCACCTGG CTCTGCAGTG 
GGATCCGTTA CTGCCACATC CTCATCGGTC GTAGTTCCCG GTGCAGCGGA CAAGAAGAAG 
ATTGAATCCT GCGCCGTTGT CGGCGGTGAG AAGGTGACGG GTAGCAAGTT GCCCGCCATT 
GGGGAAAAGA CTTCGCTCGG CGCCGTTGGC TCGGGTCCAT CAACCAGTCT TCTACTGGAC 
GCCGGCGCAC CCGTCAACAC GATTATATTT GAGAACTCCA CGTACAAGCA GCAGGCAGCT 
GCCGCTTCCG TGGTAACCAA ACCCGGAACG CTCTCGGTGT CAAGCAGTGG CGCCACCATG 
ACCGTGGACA GCCTGTCCAG CGCACTCTCC CAAATGAGTT TCGGCGGCAA GGCGACGGCG 
GCGGGCTCTG GCGATGATTC GCAGGATATG AAACTAGGAT TTAGCTTCGG TGACGATCCC 
ACCACACCCC TCAAGGTTGT GGATAACATC GATAAAACGG CCAGTCAGCA GCAGCAACAG 
CAGCAGCAAC AACACTCTCA ACAGCAACAA CAACAGCAGC AGCAGCAAAA CAATTCGACG 
GCGGATCTTA ACATGAAGAT AGCCAGCGTA AAGAAGGTTT GGGAGTCAGC CACGCCCATG 
TCCGACGGCC CATCGCCAGC ACAGGTGCAG CAGCAACAGC AACAGCAGCC CCAACAGCAA 
CAACAGTCCC AGGTGCAGGT GGCGGCGCAG CAGCAACAAC AGCAGGTGGT GGGACCGCAG 
CAGGCAGGTG ACGACGGCAG TGGTCACATG AGCGCCGCCT CCTTTGTGGC AGCCGTGGCC 
GCCTCACAGC ATCAGCAGCA ACACCAGCAA ATGCCGCACT ACGCGGTGTC GGCGGTGCAC 
ATGCAGAGCC ACCACCACCA ACAGCAGCAG CAACAGCAGC AGGCAGCGGT GGCGGCAGCG 
CAGGCGCAGC ACCACCAGCA GCAACAGGCA CAGGCGCACC ACCAGCACCA TGCACTTTCC 
TCGCCGGGCC CGGTGCCCGG TAGTCATGGC CATGGCTACA GTCATGGATC GCCGTTCGAC 
GCTGGATCGT TGGAGCAACA GTTCCAGCAA GAGGATTATC CCAGTCCACA GCAGCAACAG 
CAGGCGCACC AGAAGACCAA GCAGTTGCAG CAGCAGCAGC ATCTGGGCAT GTCACCGCCG 
CCCAGCCAGC AGCAACAGCA GCAGCACTCG CAACAGCAGC AGCAAGCACA GCACCAGCAA 
CAACAGCAGC AGGCCCATCA GCAGCAGCAA CAGCAGCAGC AGCCTTCATT CTACCAGGCG 
TCACCGCAGT TCGGCGTTGG AGGCATTCCC ACTATCCCCA GTCCGCCGGC CGTGGTGTAC 
AACTCGTCGC AGATGCCGCC ACCGTCGCAG GGGGGTAATC TGTACGCTCC ATTCCATTCG 
CTCGATCATT CGAGCCGTTC GCCCGCCTAC TCGGCGGCGG CGGCCGCCCA CAGCTACCCG 
AACCCTTATG CGGCAGCAGC AGCGGCGGCG GCGGCAGCGG CCGCTGGCGG AGGTCCCTTC 
AATGTCAACG TGAACGCATA TGCGATGCAG ACTCCGCACG GCGGCAGCCT ACCGCCCACA 
GCGCCCACAC CGGACATGTA CTCGAACCTC TCTTCGCAGT TTCGATTGGG CGGTGGTCCG 
GGTCCCGGAC CCGGTCCCTT TGGCCAGCCC AGCTCGCAGC AGTTAAGCAA TCCGAGCAAT 
CATGCTGCGG TGGCTATAAT TTCGTCAAGC AGCAACTCCA TGATGTCGTC AGGTGCGGCC 
AAACCGCCGC AGAGCCAGCA GACAATCGGT GCCATTGGCT CCAAGTCGGC AGGTAGCGGT 
GGCGGTCCAG GACCAGGACC TGGTCCCTAT GCCACCACCC AGACGTATAT GAATCTTTAT 
GCGGCTCCAC CGCCGCAACA TCCAGGACAA GGTGGACCAC CGCCGGGTGC CCATCAGCTG 
CAGTCGAACA GTTTCTACTC GAATTCGGCG CCAGGCGGTC CGAGCGGACC ACAGTTCTAC 
GGTGGTCCGC CGCCGCAGGG CAATGGTGGA GCGCAGAGCT ATGGACTGGC CACCGCGGCC 
GGCATGTATG GCGGCCATCA GGGCGGACCG CCCGGCTCCA ATGGGCCGCC GGGACCGCCG 
CAGTCGCAGC ACACGATGGG CACGTTCAAC ACGCCGTTCA TGAACTCGCA GCTGCTGACG 
GCGGCCAGCA TTAATCAGTA CCGCGGCGGA CCAACGCCTG GAGCGGCTTA TCTCAAGGGT 
AACCAGGGCC AGACACACAT GCAAGACTCG ATGGGTCGTC AGCTGAAGAG TCCGCTGAGC 
GCTGACATGA GCCTCGGTCT GGCCAAGCAG GTGCAGTCGC AGCCCAGTCC ACCGCATCAC 
AAGAACTACG GCGCGAACCA GGTGCTTCAG CAGCAGCAGC AGCAATCTCA GCAGCAACAG 
CAGCAGCAAC AGCGCCAGGT CGGCGGCGGC AATGGTCCGA GCATGAACGC CTTGGGCGGT 
CGCGGCAGTG GAGCGGTGGG CAGCGGCAGC GGCAACGGCG GAGGAGGAGG AGGAGGAGCT 
GGTGGGTCCC AAGTGGGCGG CAATGGCGGC GGTAACGGCG TTGGCAGCGT GGGCCAAAGT 
GGCGGCGGCG GCCAGGGACG CTATCCCACG CCCATTCAGC GGCCCAACAA CTATCCGCAG 
CATCCGCAAC AACAGCAGCA ACAACAGCAG CAACAGCAAC AGCAGAGAGA GCAGGCGGCA 
GCTGCCGCCG TGGCGGCTCA GCGGGCACAG AATATGCGAC AGGTGACCGG TGCCGGCGGA 
GGCGTGGCCG GAAGTGCAGG TGGCGGTGGT AGTCAAACAC CAGTGGGACC GGCCGGCGGC 
AATGGTGGCC CCGGTGGCGC AGGAGGAGGC GGTGGAGCTG GCGGTGCAGC AGGATCAGGA 
CCCGGAAAGC CCTACTATGC GAATAACGCT AGCGGAGCGG CCGTTGCGGC CAATCGAGGT 
GAGTGGCATG CGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259409.1
CDS	435..7352
Translation	MSTLGGSRGERNAKPKFTALDINRMYKNSRGESSEPSAQKNQVPRKHGMQILGKVPSARRPPANLPSLKAETHTSSNNNLLVSVEGATSGGAGAGGAAGSGAAGTNTSHNNTTAHSGSSGTSGGAGSGGGGTASSSAGGHLSQQLTHSQHQHNNNVALGPNAGKFVNSNNGSSNNNAGSSGSGNKNFKLINNSVGSSGGGGQGGSGGGGSSGVGGGSGSGSGGHSVNSPKTWSAITTGHERGSGGQNYGRQQQQVVPHYQSPQFQYEFPTLDGTVGSGAGGGSGAGVGSGSGAGKSHYQNQVHHAGHQNHHQHQQHHQQHQQHHHQQQQHHRDYHHGHGRQYGHHSHRDYRDGSDDVGGGSADLGELSLRPQNDTAAWLQQQEKAAKVAAAAAADQGLNLQGPGHHQMGGNGGAGGGSGGAVPLPILSLMPSFMRSGAPMPASGATAASILAGGSLDTSALPNAPYQNGILGARSASPAVVGSGSFRAATAIPKRPPTSSSPPQSGLGIGGSPTPPQSQQQANGGAGGAGRKDKDYVVEPEVAQMQRPIIREEDLERLNAIAKDDSWTKQDDIDFTKKLAFSDDESTPEEHQGQGKQHSSAQLQAQGSSSSTDQRKLSASTTSWQRGKESTDRDQPSEGIQDQQQQLQQQQQQQEVRQQNGHRGSSGNVGAPVAGGIALDASVYERVKQRKEEEERREMERKQAAAKKLQELEMKMNSKKAAAAALAGAEGGVSSASVPLSNEVPPTSAPLGNASEDEGGGQGGGGGLNRRPRGSISSLGSGVTPTGGAGGGGGGVSSSSSAGGDYGQKNFLTHFQSNLPPRFQRQQQQQQQQLPRLEKSASASSAFDSNSRYLQKGGVSGGGGGVSGGAQANAGQGRSISGGYVQRGASVGGSSGYGRNRHDSSSAREDQDLDQQRFTRGQDYRQAQALPRSISENSHRKTSVSSAGDDVLGGSCSSWAEQTDAEQKVHHRHREESFSSSHSHDSAVQIKILQRPARQPSLSEESSTQQKQLPASPQQQKTLTTDPMPTQILRRSVDIDKSGDERAEDKSALESDDKAKSAGKVEEDKSGNKRVSPRSGAACGGRGGRGYAGSSGGSSVGSGSSYRGQRNASDWGSSRGSGGAGGRRYYGSGGEQSEHSEDVDEECYSSGGGGAARRSPKESGNGAPNSGASANKAGFSPRGEPSRRGRGGLSSGGGASASCYRRQPVGTGSAGGSGSGGSGRSYGRLGYEEYGKQQRTSESETDLDKTKQNQLALSAGLHKPSKDDKDLPALGGEEKDKTAALAPAHNTALLAKDELQKDAAGRLPLPPGLAAPGSAVGSVTATSSSVVVPGAADKKKIESCAVVGGEKVTGSKLPAIGEKTSLGAVGSGPSTSLLLDAGAPVNTIIFENSTYKQQAAAASVVTKPGTLSVSSSGATMTVDSLSSALSQMSFGGKATAAGSGDDSQDMKLGFSFGDDPTTPLKVVDNIDKTASQQQQQQQQQHSQQQQQQQQQQNNSTADLNMKIASVKKVWESATPMSDGPSPAQVQQQQQQQPQQQQQSQVQVAAQQQQQQVVGPQQAGDDGSGHMSAASFVAAVAASQHQQQHQQMPHYAVSAVHMQSHHHQQQQQQQQAAVAAAQAQHHQQQQAQAHHQHHALSSPGPVPGSHGHGYSHGSPFDAGSLEQQFQQEDYPSPQQQQQAHQKTKQLQQQQHLGMSPPPSQQQQQQHSQQQQQAQHQQQQQQAHQQQQQQQQPSFYQASPQFGVGGIPTIPSPPAVVYNSSQMPPPSQGGNLYAPFHSLDHSSRSPAYSAAAAAHSYPNPYAAAAAAAAAAAAGGGPFNVNVNAYAMQTPHGGSLPPTAPTPDMYSNLSSQFRLGGGPGPGPGPFGQPSSQQLSNPSNHAAVAIISSSSNSMMSSGAAKPPQSQQTIGAIGSKSAGSGGGPGPGPGPYATTQTYMNLYAAPPPQHPGQGGPPPGAHQLQSNSFYSNSAPGGPSGPQFYGGPPPQGNGGAQSYGLATAAGMYGGHQGGPPGSNGPPGPPQSQHTMGTFNTPFMNSQLLTAASINQYRGGPTPGAAYLKGNQGQTHMQDSMGRQLKSPLSADMSLGLAKQVQSQPSPPHHKNYGANQVLQQQQQQSQQQQQQQQRQVGGGNGPSMNALGGRGSGAVGSGSGNGGGGGGGAGGSQVGGNGGGNGVGSVGQSGGGGQGRYPTPIQRPNNYPQHPQQQQQQQQQQQQQREQAAAAAVAAQRAQNMRQVTGAGGGVAGSAGGGGSQTPVGPAGGNGGPGGAGGGGGAGGAAGSGPGKPYYANNASGAAVAANRGEWHAN

Target ORF information:

RefSeq Version	NM_001272480.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster no circadian temperature entrainment (nocte), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272480.1

ORF Insert Sequence:

ATGAGTACAC TGGGGGGAAG TAGGGGAGAG CGAAATGCCA AGCCCAAATT CACAGCGTTA 
GATATAAATC GCATGTACAA GAATAGCCGC GGCGAGTCAA GTGAACCATC TGCACAAAAG 
AATCAAGTGC CACGTAAACA TGGAATGCAA ATCTTGGGCA AAGTGCCGTC CGCTAGGCGA 
CCACCAGCCA ATTTGCCATC CCTGAAGGCC GAGACCCACA CCAGCAGCAA CAACAATCTC 
TTAGTCTCAG TCGAAGGAGC CACATCTGGT GGGGCGGGCG CAGGCGGAGC AGCGGGATCT 
GGCGCGGCAG GAACCAACAC TAGCCACAAC AACACCACTG CCCACAGCGG AAGTTCAGGA 
ACGAGCGGCG GAGCGGGATC AGGCGGCGGA GGAACTGCGT CTTCATCTGC TGGTGGCCAT 
CTATCCCAGC AGCTGACTCA CAGCCAGCAT CAGCACAATA ATAACGTCGC TCTCGGCCCT 
AACGCCGGAA AGTTTGTCAA CAGCAACAAC GGCAGCAGCA ACAACAACGC CGGCAGCTCA 
GGCAGCGGCA ACAAGAACTT CAAATTAATC AACAACTCCG TGGGCAGTTC CGGCGGAGGC 
GGACAAGGAG GGTCGGGCGG CGGCGGATCG TCAGGAGTAG GAGGTGGATC AGGGTCAGGA 
TCGGGCGGAC ACTCAGTCAA CTCACCCAAG ACATGGTCTG CAATTACCAC AGGACACGAG 
CGGGGCTCGG GCGGTCAGAA CTATGGACGC CAGCAACAGC AGGTGGTCCC CCATTATCAG 
AGTCCACAGT TCCAGTACGA ATTTCCCACG CTGGACGGCA CAGTTGGCAG CGGCGCCGGC 
GGAGGATCGG GAGCGGGTGT GGGTTCAGGA TCTGGAGCGG GCAAGAGCCA TTACCAGAAT 
CAGGTGCACC ATGCCGGCCA CCAAAACCAT CACCAGCATC AGCAGCACCA CCAGCAGCAT 
CAACAGCACC ACCACCAGCA GCAACAGCAC CATCGGGACT ACCACCATGG CCACGGGCGC 
CAGTATGGAC ACCACTCGCA TCGCGACTAC AGAGATGGCA GCGACGACGT CGGCGGCGGG 
TCCGCGGATC TGGGCGAGCT CAGCCTTCGG CCACAGAACG ACACGGCCGC CTGGTTGCAG 
CAGCAGGAGA AGGCGGCCAA GGTGGCAGCA GCTGCGGCAG CGGACCAGGG CCTCAACCTA 
CAGGGCCCGG GCCACCATCA GATGGGCGGT AATGGCGGAG CAGGAGGAGG GTCGGGCGGA 
GCTGTGCCCC TGCCCATACT ATCGCTGATG CCCTCGTTCA TGCGTAGCGG AGCTCCGATG 
CCCGCCAGTG GAGCGACAGC GGCCAGCATC TTGGCCGGCG GATCGTTGGA CACTTCTGCG 
TTGCCCAACG CTCCCTACCA GAACGGAATC CTGGGTGCTC GGTCTGCCTC GCCAGCTGTG 
GTGGGCAGTG GAAGCTTCAG GGCGGCCACT GCCATTCCCA AGCGACCACC GACCTCCTCG 
TCGCCCCCAC AATCTGGCCT AGGCATTGGA GGATCACCCA CTCCCCCACA ATCCCAGCAG 
CAGGCCAACG GAGGCGCTGG CGGAGCCGGG CGCAAGGATA AGGACTACGT GGTGGAGCCG 
GAGGTGGCCC AAATGCAGCG TCCTATCATA CGCGAAGAGG ACTTGGAGCG CCTCAACGCC 
ATCGCCAAGG ACGATAGCTG GACCAAGCAG GACGACATTG ACTTCACGAA GAAACTAGCA 
TTCTCGGACG ATGAGTCCAC TCCGGAAGAG CACCAAGGCC AGGGCAAGCA GCACAGCTCC 
GCCCAACTGC AGGCGCAAGG ATCCTCCTCC TCAACCGATC AACGAAAGCT GTCCGCTTCC 
ACAACCAGTT GGCAGCGCGG CAAGGAGAGC ACCGATCGCG ACCAGCCATC GGAAGGCATT 
CAGGATCAGC AGCAGCAGCT TCAGCAGCAA CAGCAGCAGC AAGAGGTTCG CCAACAGAAT 
GGCCACCGCG GTAGCTCTGG CAACGTTGGA GCACCAGTGG CCGGTGGAAT TGCCTTGGAT 
GCCAGCGTGT ACGAGCGAGT AAAGCAGCGC AAGGAGGAGG AAGAGCGTCG CGAGATGGAA 
CGCAAGCAGG CGGCTGCTAA GAAGTTGCAA GAACTGGAAA TGAAGATGAA TAGCAAAAAG 
GCAGCGGCTG CGGCTCTGGC TGGAGCCGAG GGCGGCGTGT CCTCTGCCTC AGTCCCGTTG 
AGCAATGAGG TGCCACCCAC GTCCGCACCG CTAGGCAATG CCAGCGAAGA TGAGGGCGGA 
GGCCAAGGCG GCGGCGGAGG GTTGAATCGT CGACCCCGGG GAAGCATCTC TAGCTTGGGA 
AGCGGCGTAA CACCCACGGG AGGAGCTGGC GGCGGCGGCG GAGGAGTTTC GTCATCCTCG 
TCAGCAGGCG GCGACTATGG TCAAAAGAAC TTCCTTACCC ATTTCCAATC AAATTTACCA 
CCACGTTTCC AGCGCCAGCA ACAGCAACAA CAGCAGCAGC TGCCGCGCTT GGAGAAGAGC 
GCCTCGGCAA GCAGTGCATT CGATAGCAAT TCCCGCTACC TCCAGAAGGG AGGAGTAAGC 
GGAGGAGGCG GTGGTGTAAG TGGAGGAGCG CAAGCCAATG CTGGCCAGGG TCGCAGCATC 
TCCGGCGGTT ATGTGCAGCG TGGTGCAAGT GTCGGAGGAA GCAGCGGATA CGGACGAAAT 
CGTCACGACA GCAGCAGTGC TCGGGAGGAT CAGGATCTGG ATCAGCAGCG TTTCACCCGT 
GGCCAGGACT ATCGGCAGGC ACAAGCTCTG CCGCGCAGCA TATCTGAGAA CTCGCACCGT 
AAGACCAGCG TCTCATCGGC TGGCGACGAT GTGCTGGGTG GCTCGTGCTC CTCATGGGCG 
GAGCAAACGG ATGCCGAGCA GAAGGTGCAC CATCGGCACC GCGAGGAGAG CTTCTCCTCG 
TCGCACAGCC ATGACTCGGC AGTGCAAATA AAGATCCTGC AACGCCCGGC CCGACAGCCG 
AGCCTCAGTG AGGAGTCCTC GACCCAGCAG AAACAGCTGC CCGCCTCGCC GCAGCAGCAA 
AAGACGCTGA CGACAGATCC CATGCCTACC CAGATTCTGC GACGCAGCGT GGATATCGAT 
AAGTCCGGTG ATGAGCGCGC GGAGGACAAG TCTGCATTGG AATCTGATGA CAAGGCGAAG 
TCGGCTGGCA AGGTGGAGGA GGACAAGAGC GGCAATAAGC GGGTAAGTCC CAGGAGCGGA 
GCTGCTTGCG GAGGTCGAGG TGGCCGTGGT TATGCCGGCA GCTCTGGTGG ATCATCCGTC 
GGATCGGGCA GTAGCTACCG CGGCCAGAGA AATGCCAGCG ATTGGGGAAG CAGTCGTGGA 
AGCGGCGGCG CCGGCGGCAG GCGCTACTAC GGCAGCGGCG GTGAGCAATC GGAGCACTCC 
GAGGATGTGG ACGAGGAGTG TTACAGCAGT GGAGGAGGCG GAGCCGCTCG TCGCAGCCCA 
AAGGAATCGG GCAACGGTGC GCCCAATTCC GGTGCGTCGG CAAACAAGGC AGGATTCTCG 
CCACGCGGCG AACCATCTAG ACGTGGTCGA GGCGGCCTCT CTAGCGGAGG AGGCGCCTCT 
GCTTCTTGTT ACCGTCGACA GCCCGTGGGC ACTGGATCCG CTGGTGGATC TGGTTCGGGC 
GGATCGGGCC GCAGCTATGG CAGACTTGGC TACGAGGAAT ATGGCAAGCA ACAGCGAACT 
TCCGAGTCGG AGACGGACCT AGACAAGACC AAGCAGAACC AGCTGGCACT CAGCGCCGGC 
CTGCACAAAC CAAGCAAGGA TGACAAGGAT TTGCCTGCGC TTGGGGGCGA GGAGAAGGAC 
AAGACTGCCG CCCTGGCGCC TGCCCATAAC ACTGCCCTGC TAGCAAAGGA TGAATTACAG 
AAAGATGCAG CGGGTCGCCT GCCGTTGCCT CCCGGCCTCG CAGCACCTGG CTCTGCAGTG 
GGATCCGTTA CTGCCACATC CTCATCGGTC GTAGTTCCCG GTGCAGCGGA CAAGAAGAAG 
ATTGAATCCT GCGCCGTTGT CGGCGGTGAG AAGGTGACGG GTAGCAAGTT GCCCGCCATT 
GGGGAAAAGA CTTCGCTCGG CGCCGTTGGC TCGGGTCCAT CAACCAGTCT TCTACTGGAC 
GCCGGCGCAC CCGTCAACAC GATTATATTT GAGAACTCCA CGTACAAGCA GCAGGCAGCT 
GCCGCTTCCG TGGTAACCAA ACCCGGAACG CTCTCGGTGT CAAGCAGTGG CGCCACCATG 
ACCGTGGACA GCCTGTCCAG CGCACTCTCC CAAATGAGTT TCGGCGGCAA GGCGACGGCG 
GCGGGCTCTG GCGATGATTC GCAGGATATG AAACTAGGAT TTAGCTTCGG TGACGATCCC 
ACCACACCCC TCAAGGTTGT GGATAACATC GATAAAACGG CCAGTCAGCA GCAGCAACAG 
CAGCAGCAAC AACACTCTCA ACAGCAACAA CAACAGCAGC AGCAGCAAAA CAATTCGACG 
GCGGATCTTA ACATGAAGAT AGCCAGCGTA AAGAAGGTTT GGGAGTCAGC CACGCCCATG 
TCCGACGGCC CATCGCCAGC ACAGGTGCAG CAGCAACAGC AACAGCAGCC CCAACAGCAA 
CAACAGTCCC AGGTGCAGGT GGCGGCGCAG CAGCAACAAC AGCAGGTGGT GGGACCGCAG 
CAGGCAGGTG ACGACGGCAG TGGTCACATG AGCGCCGCCT CCTTTGTGGC AGCCGTGGCC 
GCCTCACAGC ATCAGCAGCA ACACCAGCAA ATGCCGCACT ACGCGGTGTC GGCGGTGCAC 
ATGCAGAGCC ACCACCACCA ACAGCAGCAG CAACAGCAGC AGGCAGCGGT GGCGGCAGCG 
CAGGCGCAGC ACCACCAGCA GCAACAGGCA CAGGCGCACC ACCAGCACCA TGCACTTTCC 
TCGCCGGGCC CGGTGCCCGG TAGTCATGGC CATGGCTACA GTCATGGATC GCCGTTCGAC 
GCTGGATCGT TGGAGCAACA GTTCCAGCAA GAGGATTATC CCAGTCCACA GCAGCAACAG 
CAGGCGCACC AGAAGACCAA GCAGTTGCAG CAGCAGCAGC ATCTGGGCAT GTCACCGCCG 
CCCAGCCAGC AGCAACAGCA GCAGCACTCG CAACAGCAGC AGCAAGCACA GCACCAGCAA 
CAACAGCAGC AGGCCCATCA GCAGCAGCAA CAGCAGCAGC AGCCTTCATT CTACCAGGCG 
TCACCGCAGT TCGGCGTTGG AGGCATTCCC ACTATCCCCA GTCCGCCGGC CGTGGTGTAC 
AACTCGTCGC AGATGCCGCC ACCGTCGCAG GGGGGTAATC TGTACGCTCC ATTCCATTCG 
CTCGATCATT CGAGCCGTTC GCCCGCCTAC TCGGCGGCGG CGGCCGCCCA CAGCTACCCG 
AACCCTTATG CGGCAGCAGC AGCGGCGGCG GCGGCAGCGG CCGCTGGCGG AGGTCCCTTC 
AATGTCAACG TGAACGCATA TGCGATGCAG ACTCCGCACG GCGGCAGCCT ACCGCCCACA 
GCGCCCACAC CGGACATGTA CTCGAACCTC TCTTCGCAGT TTCGATTGGG CGGTGGTCCG 
GGTCCCGGAC CCGGTCCCTT TGGCCAGCCC AGCTCGCAGC AGTTAAGCAA TCCGAGCAAT 
CATGCTGCGG TGGCTATAAT TTCGTCAAGC AGCAACTCCA TGATGTCGTC AGGTGCGGCC 
AAACCGCCGC AGAGCCAGCA GACAATCGGT GCCATTGGCT CCAAGTCGGC AGGTAGCGGT 
GGCGGTCCAG GACCAGGACC TGGTCCCTAT GCCACCACCC AGACGTATAT GAATCTTTAT 
GCGGCTCCAC CGCCGCAACA TCCAGGACAA GGTGGACCAC CGCCGGGTGC CCATCAGCTG 
CAGTCGAACA GTTTCTACTC GAATTCGGCG CCAGGCGGTC CGAGCGGACC ACAGTTCTAC 
GGTGGTCCGC CGCCGCAGGG CAATGGTGGA GCGCAGAGCT ATGGACTGGC CACCGCGGCC 
GGCATGTATG GCGGCCATCA GGGCGGACCG CCCGGCTCCA ATGGGCCGCC GGGACCGCCG 
CAGTCGCAGC ACACGATGGG CACGTTCAAC ACGCCGTTCA TGAACTCGCA GCTGCTGACG 
GCGGCCAGCA TTAATCAGTA CCGCGGCGGA CCAACGCCTG GAGCGGCTTA TCTCAAGGGT 
AACCAGGGCC AGACACACAT GCAAGACTCG ATGGGTCGTC AGCTGAAGAG TCCGCTGAGC 
GCTGACATGA GCCTCGGTCT GGCCAAGCAG GTGCAGTCGC AGCCCAGTCC ACCGCATCAC 
AAGAACTACG GCGCGAACCA GGTGCTTCAG CAGCAGCAGC AGCAATCTCA GCAGCAACAG 
CAGCAGCAAC AGCGCCAGGT CGGCGGCGGC AATGGTCCGA GCATGAACGC CTTGGGCGGT 
CGCGGCAGTG GAGCGGTGGG CAGCGGCAGC GGCAACGGCG GAGGAGGAGG AGGAGGAGCT 
GGTGGGTCCC AAGTGGGCGG CAATGGCGGC GGTAACGGCG TTGGCAGCGT GGGCCAAAGT 
GGCGGCGGCG GCCAGGGACG CTATCCCACG CCCATTCAGC GGCCCAACAA CTATCCGCAG 
CATCCGCAAC AACAGCAGCA ACAACAGCAG CAACAGCAAC AGCAGAGAGA GCAGGCGGCA 
GCTGCCGCCG TGGCGGCTCA GCGGGCACAG AATATGCGAC AGGTGACCGG TGCCGGCGGA 
GGCGTGGCCG GAAGTGCAGG TGGCGGTGGT AGTCAAACAC CAGTGGGACC GGCCGGCGGC 
AATGGTGGCC CCGGTGGCGC AGGAGGAGGC GGTGGAGCTG GCGGTGCAGC AGGATCAGGA 
CCCGGAAAGC CCTACTATGC GAATAACGCT AGCGGAGCGG CCGTTGCGGC CAATCGAGGT 
GAGTGGCATG CGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nocte cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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