CG4557 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG4557
Entrez Gene ID	31629
Full Name	CG4557 gene product from transcript CG4557-RA
Synonyms	AAF46211,Dmel\CG4557
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 6D5-6D6

mRNA and Protein(s)

mRNA	Protein	Name
NM_132132.3	NP_572360.1	uncharacterized protein

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG4557	NP_572360.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000660	XP_311186.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG4557 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG4557 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00982	NM_132132.3 Latest version!	Drosophila melanogaster uncharacterized protein (CG4557), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG4557 ( NM_132132.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572360.1 Drosophila melanogaster uncharacterized protein (CG4557), mRNA.

Price & Availability↑

CloneID	OFa00982
Clone ID Related Accession (Same CDS sequence)	NM_132132.3
Accession Version	NM_132132.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132132.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTGGT TCGAAAAGGC CAAAACGGTG CTAATTGAGG CGCTGGACAT CCAGGATGAC 
GACAGCAAGG CGGCGGAGAA GGACTCGCCG ATGGCAGGAG CTGCGAGCGA TGGTGGCGCA 
TCGCCTGGGA ACAATGCAAC GGCGGAGACA CCTGGATCCG CTGCCACGCC CGCTTCGGAT 
ACGCCAACAT TCTTTGACAA TCCGCATGCC AACGAAATGG TGACCATTCC ACCCACACCC 
ACATCGGTGC CAGGAAGTGC CGCCTACGGC AAACGGCAGT CAGCCACCTC GGATTCGGTG 
GATCTGCTGT CCTCCCCAAC CTCGCCCATC TCGCCCTGCG GCGGCGATGT CCCCTCGGTG 
GCCAAGCGCT TGTCTGAATC CTCGCTAGAA CTCATAACGG CCACCACGGC CAGCGAAGTG 
GAGATGTCAC CGGACACGGA GCCCTCCTTG CCCGACAGCA TTGTGATAAT CGCCAGCAAT 
GACACATCCG ACAATGCCGA GGGTGATGGC GAGGATGATG ACGATGAGGG CACCCAGCTG 
AAGCGCCACA TGGAGGACCA CTTGAATGAC TCCACCAAAA CCATGAAGGC TCTGGTCCTG 
AGCGACATCC AGGTAGCCGG TAATGCTGAC TCCGATTCCA CGCAGAGCTT CGAGGACATC 
CAGCTGCAGA TGAGCCACAA GGGCAAGTCG CCCGCTCCGG CCAAGACGGG AACGGTGGAC 
CAGAGCTACT CGTCCACCTC ATCGGACATC GAGATCATCT CAAATCCGAA TGGCGACTCC 
AGCACCAACA GCACCACCAC GCGCACCAGC CCGCAAAAAT TCAAGGAGCT CGCGGGCGGC 
AAGGTGTGCC TAAAGGCCAA GGGACATCAT CGCGAGCCCT CGGAGATCTC GCTGCTGTCG 
GAGGACTCAC AGTCGGAGCT GGATAAGCTG GTGCAACGCA TCAGCGAGCT GAACCAGGTG 
ATTGAGGCGC GGGAGCAGCG ACTATTGCAA TCAGAGCGCC AGAATGCCGA GCTCCTCGAG 
CGCAACCAGG AGTTGCGCGC TCGCGTCGAA GCGGCAGCCA ATAGCGCCAA CAGTCCGGAT 
GCGGCGGATG CAGTGCAGCG TCTGTCGGCG CTGGAGAAGA AGTTCCAGGC CAGCATACGC 
GAGCGCGATG CCCTGCGCAT CCAAATGAAG AGTCTGCGCG ATGAGCTGCA GAACAAGATA 
CCCAGGGATG AGCTAGCCGA ATGCAACGAA ATGATCGCCG CCCTGCAGTC CGAGGGTGAG 
AAGCTGTCCA AGGAGATCCT GCAGCAGTCG ACGATCATCA AGAAACTGCG CGCCAAGGAG 
AAGACCTCGG ACACGTTGCT GAAGAAGAAC GGCGAGCAGA TCTCGTTGCT GTCCAGCGAA 
TCGGAGCGCC TCAAGAGATC CCTAGCCGCC AAGGAGGAAA TGGAGCGCAC GCAAATCGAG 
GCAGTTTGCC GGATGACGGC GGAGAAGAAG CGCGTGGATG AGGAGAACGC CGAGAGCCGC 
AGTCGCATCG AAGATCTTCA GTCCAGACTA GCTGCCCTGC AAGCCAGTTT CGACGGCCTA 
AAGGGAGATC TGCAGAAGCG CACGCGCCTG GAGCAGGACA GTCTGCGTGC CGAGCATCAG 
GAGTACGTGC AGCAGGTCTC CGATCTGCGC GAGAAACTGC GCCTGGCCGA GCACAGCCTG 
GCCAGAAGGG AGCAGCAAAT GCGCGAGGAG AACCGCCAGC TAATGCGTCG CCTGGAAGCC 
GCCGAGCTGC GGGCGGAGAG CTCTACTCAA GAGCTGGGTG CCACTACCAC GCCGTTGATA 
CGACAGATCG AGTCGCTGCA GCGTACACTG GACCAGCGAT CCGCTGCCTG GAACCGCGAG 
GAGCAGCAGC TCCTCCAGAA ACTGGACGAC TCACAGGTGC AACTGCGCTC GCTGCAGCAA 
TTGGAATCCG TACAGGGCGA GAAGCAGGAG CTGCTGCGCA CCCGTTGCGG CCTGCTGGAG 
GAGAAGCTGT CCAGTGCGCT AATGGAGGCG GAGGCCGCCA AGATGGCACT GCGTCAGCAC 
GATCTGGAAG CGGCCAACAA GGAAAACGAT CACAAAAAAC AATTGAGCCT GCTGCAGGAG 
GAGATTCAGC AGCAGCAGGA GAGGATTGCG AGTCTGGAGC AATTGTGCCA GCGGCAAAAG 
GAAGAGGAGG AGCAGCGGAA ACAGCCCACG CTGCTGACAG TGAAGGCGGT CAAGGCTAGC 
AGTGAGTTGC AGCCACAGCT GCAAATGCAG CTGCCGAAAT CCCAGGCACC ACTGCGCTCC 
TCACCGCCCC TCAGTTTGGT GGACGACAGT GGTTCCAACG AGGAGGCCCT GGGCGGCATG 
ATCGACTGGC AGGCGGACGA CCTCGATTGC GCTTCCAACT CGGGTCGCCA GCAATCGGGC 
ATCATCCAAG GTGTACACCT GAGCTTTCTG GCGGCCAACA CAACGACCTT TGAGCACCTG 
CAGGCTCTGC TCAAGCAGCG CGACGGGGAG CTGACCCACC TGCAGTGGGA AGTGTCGCGC 
CTGCAAGCAG AGCGAAGCGT TCTCGACGCC GAGATATCCA ATCTGACCAT CGAGCTGGAA 
ACGATGAAGG AGAAGCAGCA GATGTACGAG GTAATGGAGA AGGGATACGA GGATCTGCAG 
CATCGCTACG ACGCCCTGCT CCAGATGTAC GGCGAGAAGG TGGAGCGCAC CGAGGAACTG 
GAGCTGGATC TGACCGAGCT GAAGGCGGCC TACAAGCTGC AGATCGACGA GCTGCTGGCC 
GCCCCGCCAC CCAATCTGCA ACGGCCGTCG AAGCAAACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572360.1
CDS	267..3068
Translation	MSWFEKAKTVLIEALDIQDDDSKAAEKDSPMAGAASDGGASPGNNATAETPGSAATPASDTPTFFDNPHANEMVTIPPTPTSVPGSAAYGKRQSATSDSVDLLSSPTSPISPCGGDVPSVAKRLSESSLELITATTASEVEMSPDTEPSLPDSIVIIASNDTSDNAEGDGEDDDDEGTQLKRHMEDHLNDSTKTMKALVLSDIQVAGNADSDSTQSFEDIQLQMSHKGKSPAPAKTGTVDQSYSSTSSDIEIISNPNGDSSTNSTTTRTSPQKFKELAGGKVCLKAKGHHREPSEISLLSEDSQSELDKLVQRISELNQVIEAREQRLLQSERQNAELLERNQELRARVEAAANSANSPDAADAVQRLSALEKKFQASIRERDALRIQMKSLRDELQNKIPRDELAECNEMIAALQSEGEKLSKEILQQSTIIKKLRAKEKTSDTLLKKNGEQISLLSSESERLKRSLAAKEEMERTQIEAVCRMTAEKKRVDEENAESRSRIEDLQSRLAALQASFDGLKGDLQKRTRLEQDSLRAEHQEYVQQVSDLREKLRLAEHSLARREQQMREENRQLMRRLEAAELRAESSTQELGATTTPLIRQIESLQRTLDQRSAAWNREEQQLLQKLDDSQVQLRSLQQLESVQGEKQELLRTRCGLLEEKLSSALMEAEAAKMALRQHDLEAANKENDHKKQLSLLQEEIQQQQERIASLEQLCQRQKEEEEQRKQPTLLTVKAVKASSELQPQLQMQLPKSQAPLRSSPPLSLVDDSGSNEEALGGMIDWQADDLDCASNSGRQQSGIIQGVHLSFLAANTTTFEHLQALLKQRDGELTHLQWEVSRLQAERSVLDAEISNLTIELETMKEKQQMYEVMEKGYEDLQHRYDALLQMYGEKVERTEELELDLTELKAAYKLQIDELLAAPPPNLQRPSKQT

Target ORF information:

RefSeq Version	NM_132132.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG4557), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132132.3

ORF Insert Sequence:

ATGAGCTGGT TCGAAAAGGC CAAAACGGTG CTAATTGAGG CGCTGGACAT CCAGGATGAC 
GACAGCAAGG CGGCGGAGAA GGACTCGCCG ATGGCAGGAG CTGCGAGCGA TGGTGGCGCA 
TCGCCTGGGA ACAATGCAAC GGCGGAGACA CCTGGATCCG CTGCCACGCC CGCTTCGGAT 
ACGCCAACAT TCTTTGACAA TCCGCATGCC AACGAAATGG TGACCATTCC ACCCACACCC 
ACATCGGTGC CAGGAAGTGC CGCCTACGGC AAACGGCAGT CAGCCACCTC GGATTCGGTG 
GATCTGCTGT CCTCCCCAAC CTCGCCCATC TCGCCCTGCG GCGGCGATGT CCCCTCGGTG 
GCCAAGCGCT TGTCTGAATC CTCGCTAGAA CTCATAACGG CCACCACGGC CAGCGAAGTG 
GAGATGTCAC CGGACACGGA GCCCTCCTTG CCCGACAGCA TTGTGATAAT CGCCAGCAAT 
GACACATCCG ACAATGCCGA GGGTGATGGC GAGGATGATG ACGATGAGGG CACCCAGCTG 
AAGCGCCACA TGGAGGACCA CTTGAATGAC TCCACCAAAA CCATGAAGGC TCTGGTCCTG 
AGCGACATCC AGGTAGCCGG TAATGCTGAC TCCGATTCCA CGCAGAGCTT CGAGGACATC 
CAGCTGCAGA TGAGCCACAA GGGCAAGTCG CCCGCTCCGG CCAAGACGGG AACGGTGGAC 
CAGAGCTACT CGTCCACCTC ATCGGACATC GAGATCATCT CAAATCCGAA TGGCGACTCC 
AGCACCAACA GCACCACCAC GCGCACCAGC CCGCAAAAAT TCAAGGAGCT CGCGGGCGGC 
AAGGTGTGCC TAAAGGCCAA GGGACATCAT CGCGAGCCCT CGGAGATCTC GCTGCTGTCG 
GAGGACTCAC AGTCGGAGCT GGATAAGCTG GTGCAACGCA TCAGCGAGCT GAACCAGGTG 
ATTGAGGCGC GGGAGCAGCG ACTATTGCAA TCAGAGCGCC AGAATGCCGA GCTCCTCGAG 
CGCAACCAGG AGTTGCGCGC TCGCGTCGAA GCGGCAGCCA ATAGCGCCAA CAGTCCGGAT 
GCGGCGGATG CAGTGCAGCG TCTGTCGGCG CTGGAGAAGA AGTTCCAGGC CAGCATACGC 
GAGCGCGATG CCCTGCGCAT CCAAATGAAG AGTCTGCGCG ATGAGCTGCA GAACAAGATA 
CCCAGGGATG AGCTAGCCGA ATGCAACGAA ATGATCGCCG CCCTGCAGTC CGAGGGTGAG 
AAGCTGTCCA AGGAGATCCT GCAGCAGTCG ACGATCATCA AGAAACTGCG CGCCAAGGAG 
AAGACCTCGG ACACGTTGCT GAAGAAGAAC GGCGAGCAGA TCTCGTTGCT GTCCAGCGAA 
TCGGAGCGCC TCAAGAGATC CCTAGCCGCC AAGGAGGAAA TGGAGCGCAC GCAAATCGAG 
GCAGTTTGCC GGATGACGGC GGAGAAGAAG CGCGTGGATG AGGAGAACGC CGAGAGCCGC 
AGTCGCATCG AAGATCTTCA GTCCAGACTA GCTGCCCTGC AAGCCAGTTT CGACGGCCTA 
AAGGGAGATC TGCAGAAGCG CACGCGCCTG GAGCAGGACA GTCTGCGTGC CGAGCATCAG 
GAGTACGTGC AGCAGGTCTC CGATCTGCGC GAGAAACTGC GCCTGGCCGA GCACAGCCTG 
GCCAGAAGGG AGCAGCAAAT GCGCGAGGAG AACCGCCAGC TAATGCGTCG CCTGGAAGCC 
GCCGAGCTGC GGGCGGAGAG CTCTACTCAA GAGCTGGGTG CCACTACCAC GCCGTTGATA 
CGACAGATCG AGTCGCTGCA GCGTACACTG GACCAGCGAT CCGCTGCCTG GAACCGCGAG 
GAGCAGCAGC TCCTCCAGAA ACTGGACGAC TCACAGGTGC AACTGCGCTC GCTGCAGCAA 
TTGGAATCCG TACAGGGCGA GAAGCAGGAG CTGCTGCGCA CCCGTTGCGG CCTGCTGGAG 
GAGAAGCTGT CCAGTGCGCT AATGGAGGCG GAGGCCGCCA AGATGGCACT GCGTCAGCAC 
GATCTGGAAG CGGCCAACAA GGAAAACGAT CACAAAAAAC AATTGAGCCT GCTGCAGGAG 
GAGATTCAGC AGCAGCAGGA GAGGATTGCG AGTCTGGAGC AATTGTGCCA GCGGCAAAAG 
GAAGAGGAGG AGCAGCGGAA ACAGCCCACG CTGCTGACAG TGAAGGCGGT CAAGGCTAGC 
AGTGAGTTGC AGCCACAGCT GCAAATGCAG CTGCCGAAAT CCCAGGCACC ACTGCGCTCC 
TCACCGCCCC TCAGTTTGGT GGACGACAGT GGTTCCAACG AGGAGGCCCT GGGCGGCATG 
ATCGACTGGC AGGCGGACGA CCTCGATTGC GCTTCCAACT CGGGTCGCCA GCAATCGGGC 
ATCATCCAAG GTGTACACCT GAGCTTTCTG GCGGCCAACA CAACGACCTT TGAGCACCTG 
CAGGCTCTGC TCAAGCAGCG CGACGGGGAG CTGACCCACC TGCAGTGGGA AGTGTCGCGC 
CTGCAAGCAG AGCGAAGCGT TCTCGACGCC GAGATATCCA ATCTGACCAT CGAGCTGGAA 
ACGATGAAGG AGAAGCAGCA GATGTACGAG GTAATGGAGA AGGGATACGA GGATCTGCAG 
CATCGCTACG ACGCCCTGCT CCAGATGTAC GGCGAGAAGG TGGAGCGCAC CGAGGAACTG 
GAGCTGGATC TGACCGAGCT GAAGGCGGCC TACAAGCTGC AGATCGACGA GCTGCTGGCC 
GCCCCGCCAC CCAATCTGCA ACGGCCGTCG AAGCAAACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG4557 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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