Ctr1A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ctr1A
Entrez Gene ID	31601
Full Name	Copper transporter 1A
Synonyms	BcDNA:RH42635,CG3977,DmCtr1A,Dmel\CG3977,ctr1A
General protein information	Preferred Names Copper transporter 1A Names CG3977 gene product from transcript CG3977-RA CG3977-PA CG3977-PB CG3977-PC CG3977-PD Ctr1A-PA Ctr1A-PB Ctr1A-PC Ctr1A-PD copper transporter 1A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 6C4-6C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_132108.3	NP_572336.2	copper transporter 1A, isoform A
NM_001258622.2	NP_001245551.1	copper transporter 1A, isoform B
NM_001258623.2	NP_001245552.1	copper transporter 1A, isoform C
NM_001258624.1	NP_001245553.1	copper transporter 1A, isoform D

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SLC31A1	XP_538800.1
	SLC31A1	XP_001103110.1
Mus musculus (house mouse)	Slc31a1	NP_780299.2
Drosophila melanogaster (fruit fly)	Ctr1A	NP_001245552.1
Gallus gallus (chicken)	SLC31A1	XP_004945826.1
Danio rerio (zebrafish)	slc31a1	NP_991280.1
Homo sapiens (human)	SLC31A1	NP_001850.1
Rattus norvegicus (Norway rat)	Slc31a1	NP_598284.1
Pan troglodytes (chimpanzee)	SLC31A1	XP_520197.2
Bos taurus (cattle)	SLC31A1	NP_001093851.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Syx5 plays an essential role in copper homeostasis through Ctr1A and is a candidate gene for copper-related disease in humans
Title: Syntaxin 5 is required for copper homeostasis in Drosophila and mammals.

in Drosophila, the autosomal Ctr1C gene originated as a retrogene copy of the X-linked Ctr1A, thus maintaining copper delivery during male spermatogenesis
Title: The Drosophila copper transporter Ctr1C functions in male fertility.

distinctions between two Drosophila plasma membrane copper transport proteins; demonstrate that copper import by Ctr1A is required to drive neuropeptide maturation during normal growth and development.
Title: Drosophila Ctr1A functions as a copper transporter essential for development.

The following Ctr1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ctr1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00955	NM_001258624.1 Latest version!	Drosophila melanogaster copper transporter 1A, transcript variant D (Ctr1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00955	NM_001258623.2 Latest version!	Drosophila melanogaster copper transporter 1A, transcript variant C (Ctr1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00956	NM_001258622.2 Latest version!	Drosophila melanogaster copper transporter 1A, transcript variant B (Ctr1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00956	NM_132108.3 Latest version!	Drosophila melanogaster copper transporter 1A, transcript variant A (Ctr1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ctr1A ( NM_001258623.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245552.1 Drosophila melanogaster copper transporter 1A, transcript variant C (Ctr1A), mRNA.

Price & Availability↑

CloneID	OFa00955
Clone ID Related Accession (Same CDS sequence)	NM_001258623.2 , NM_001258624.1
Accession Version	NM_001258623.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	copper transporter 1A, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258623.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCACG CCCATCACAG TGCGCCCGGT GTTGATCACT CGATGCATCA CGATCATGTG 
GGCATGCACC ACGATCACAG CGGTATACCT GCTGCGACAG CGTCTCCAAT GGATGCAGCT 
TCCATGTTCG ACCTGATCCC CGATACCAGC GATTTGCAGG CAAGTCACGC GGGTCACGCC 
GCTCACGGAG CGCACAATCA TGGCGGTGGC TCTGGCACGG GAATGGAGCA CATGATGCCC 
ATGGCGTTCC ATTTTGGCTA CAACGAGACG ATCCTGTTCT CCTGGTGGCA CATCGAAACA 
GTGGCCGGTT TGATCGGCTC CATGATCGCC ATCTTCCTGC TGGCCCTAAT GTACGAGGGA 
CTGAAGTACT ACCGCGAGTA TCTATTCTGG AAGACATACA ATCTGCTGGA ATATCGACCT 
GTGACGGGGC CACAAAGAAA TCCGGAGGCA CCGCGCATAC CATCGCCGGC AGCGGCAGCT 
CCATCGCCCG TTCAATACGT GGGCGAAGTT GTGCACAAGC AACCACCTTC GATGCTGTCG 
ATTAACCACC TGCTCCAGAC ACTGCTGCAC GTGCTCCAGG TGACGCTGTC CTTCCTGCTC 
ATGCTGATCT TCATGACATA CAACGTGTGG CTCTGCCTGA TGGTTGTCCT GGGCGCCGCC 
GTTGGTTACT TCCTCTTCTG CTGGAAGAAA TCGGTTATCG TGGACGTAAC CGAGCACTGT 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245552.1
CDS	299..1024
Translation	MDHAHHSAPGVDHSMHHDHVGMHHDHSGIPAATASPMDAASMFDLIPDTSDLQASHAGHAAHGAHNHGGGSGTGMEHMMPMAFHFGYNETILFSWWHIETVAGLIGSMIAIFLLALMYEGLKYYREYLFWKTYNLLEYRPVTGPQRNPEAPRIPSPAAAAPSPVQYVGEVVHKQPPSMLSINHLLQTLLHVLQVTLSFLLMLIFMTYNVWLCLMVVLGAAVGYFLFCWKKSVIVDVTEHCH

Target ORF information:

RefSeq Version	NM_001258623.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster copper transporter 1A, transcript variant C (Ctr1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258623.2

ORF Insert Sequence:

ATGGACCACG CCCATCACAG TGCGCCCGGT GTTGATCACT CGATGCATCA CGATCATGTG 
GGCATGCACC ACGATCACAG CGGTATACCT GCTGCGACAG CGTCTCCAAT GGATGCAGCT 
TCCATGTTCG ACCTGATCCC CGATACCAGC GATTTGCAGG CAAGTCACGC GGGTCACGCC 
GCTCACGGAG CGCACAATCA TGGCGGTGGC TCTGGCACGG GAATGGAGCA CATGATGCCC 
ATGGCGTTCC ATTTTGGCTA CAACGAGACG ATCCTGTTCT CCTGGTGGCA CATCGAAACA 
GTGGCCGGTT TGATCGGCTC CATGATCGCC ATCTTCCTGC TGGCCCTAAT GTACGAGGGA 
CTGAAGTACT ACCGCGAGTA TCTATTCTGG AAGACATACA ATCTGCTGGA ATATCGACCT 
GTGACGGGGC CACAAAGAAA TCCGGAGGCA CCGCGCATAC CATCGCCGGC AGCGGCAGCT 
CCATCGCCCG TTCAATACGT GGGCGAAGTT GTGCACAAGC AACCACCTTC GATGCTGTCG 
ATTAACCACC TGCTCCAGAC ACTGCTGCAC GTGCTCCAGG TGACGCTGTC CTTCCTGCTC 
ATGCTGATCT TCATGACATA CAACGTGTGG CTCTGCCTGA TGGTTGTCCT GGGCGCCGCC 
GTTGGTTACT TCCTCTTCTG CTGGAAGAAA TCGGTTATCG TGGACGTAAC CGAGCACTGT 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctr1A ( NM_001258624.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245553.1 Drosophila melanogaster copper transporter 1A, transcript variant D (Ctr1A), mRNA.

Price & Availability↑

CloneID	OFa00955
Clone ID Related Accession (Same CDS sequence)	NM_001258623.2 , NM_001258624.1
Accession Version	NM_001258624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	copper transporter 1A, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCACG CCCATCACAG TGCGCCCGGT GTTGATCACT CGATGCATCA CGATCATGTG 
GGCATGCACC ACGATCACAG CGGTATACCT GCTGCGACAG CGTCTCCAAT GGATGCAGCT 
TCCATGTTCG ACCTGATCCC CGATACCAGC GATTTGCAGG CAAGTCACGC GGGTCACGCC 
GCTCACGGAG CGCACAATCA TGGCGGTGGC TCTGGCACGG GAATGGAGCA CATGATGCCC 
ATGGCGTTCC ATTTTGGCTA CAACGAGACG ATCCTGTTCT CCTGGTGGCA CATCGAAACA 
GTGGCCGGTT TGATCGGCTC CATGATCGCC ATCTTCCTGC TGGCCCTAAT GTACGAGGGA 
CTGAAGTACT ACCGCGAGTA TCTATTCTGG AAGACATACA ATCTGCTGGA ATATCGACCT 
GTGACGGGGC CACAAAGAAA TCCGGAGGCA CCGCGCATAC CATCGCCGGC AGCGGCAGCT 
CCATCGCCCG TTCAATACGT GGGCGAAGTT GTGCACAAGC AACCACCTTC GATGCTGTCG 
ATTAACCACC TGCTCCAGAC ACTGCTGCAC GTGCTCCAGG TGACGCTGTC CTTCCTGCTC 
ATGCTGATCT TCATGACATA CAACGTGTGG CTCTGCCTGA TGGTTGTCCT GGGCGCCGCC 
GTTGGTTACT TCCTCTTCTG CTGGAAGAAA TCGGTTATCG TGGACGTAAC CGAGCACTGT 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245553.1
CDS	201..926
Translation	MDHAHHSAPGVDHSMHHDHVGMHHDHSGIPAATASPMDAASMFDLIPDTSDLQASHAGHAAHGAHNHGGGSGTGMEHMMPMAFHFGYNETILFSWWHIETVAGLIGSMIAIFLLALMYEGLKYYREYLFWKTYNLLEYRPVTGPQRNPEAPRIPSPAAAAPSPVQYVGEVVHKQPPSMLSINHLLQTLLHVLQVTLSFLLMLIFMTYNVWLCLMVVLGAAVGYFLFCWKKSVIVDVTEHCH

Target ORF information:

RefSeq Version	NM_001258624.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster copper transporter 1A, transcript variant D (Ctr1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258624.1

ORF Insert Sequence:

ATGGACCACG CCCATCACAG TGCGCCCGGT GTTGATCACT CGATGCATCA CGATCATGTG 
GGCATGCACC ACGATCACAG CGGTATACCT GCTGCGACAG CGTCTCCAAT GGATGCAGCT 
TCCATGTTCG ACCTGATCCC CGATACCAGC GATTTGCAGG CAAGTCACGC GGGTCACGCC 
GCTCACGGAG CGCACAATCA TGGCGGTGGC TCTGGCACGG GAATGGAGCA CATGATGCCC 
ATGGCGTTCC ATTTTGGCTA CAACGAGACG ATCCTGTTCT CCTGGTGGCA CATCGAAACA 
GTGGCCGGTT TGATCGGCTC CATGATCGCC ATCTTCCTGC TGGCCCTAAT GTACGAGGGA 
CTGAAGTACT ACCGCGAGTA TCTATTCTGG AAGACATACA ATCTGCTGGA ATATCGACCT 
GTGACGGGGC CACAAAGAAA TCCGGAGGCA CCGCGCATAC CATCGCCGGC AGCGGCAGCT 
CCATCGCCCG TTCAATACGT GGGCGAAGTT GTGCACAAGC AACCACCTTC GATGCTGTCG 
ATTAACCACC TGCTCCAGAC ACTGCTGCAC GTGCTCCAGG TGACGCTGTC CTTCCTGCTC 
ATGCTGATCT TCATGACATA CAACGTGTGG CTCTGCCTGA TGGTTGTCCT GGGCGCCGCC 
GTTGGTTACT TCCTCTTCTG CTGGAAGAAA TCGGTTATCG TGGACGTAAC CGAGCACTGT 
CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctr1A ( NM_132108.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572336.2 Drosophila melanogaster copper transporter 1A, transcript variant A (Ctr1A), mRNA.

Price & Availability↑

CloneID	OFa00956
Clone ID Related Accession (Same CDS sequence)	NM_132108.3 , NM_001258622.2
Accession Version	NM_132108.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	copper transporter 1A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_132108.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCACG CCCATCACAG TGCGCCCGGT GTTGATCACT CGATGCATCA CGATCATGTG 
GGCATGCACC ACGATCACAG CGGTATACCT GCTGCGACAG CGTCTCCAAT GGATGCAGCT 
TCCATGTTCG ACCTGATCCC CGATACCAGC GATTTGCAGG CAAGTCACGC GGGTCACGCC 
GCTCACGGAG CGCACAATCA TGGCGGTGGC TCTGGCACGG GAATGGAGCA CATGATGCCC 
ATGGCGTTCC ATTTTGGCTA CAACGAGACG ATCCTGTTCT CCTGGTGGCA CATCGAAACA 
GTGGCCGGTT TGATCGGCTC CATGATCGCC ATCTTCCTGC TGGCCCTAAT GTACGAGGGA 
CTGAAGTACT ACCGCGAGTA TCTATTCTGG AAGACATACA ATCTGCTGGA ATATCGACCT 
GTGACGGGGC CACAAAGAAA TCCGGAGGCA CCGCGCATAC CATCGCCGGC AGCGGCAGCT 
CCATCGCCCG TTCAACCTTC GATGCTGTCG ATTAACCACC TGCTCCAGAC ACTGCTGCAC 
GTGCTCCAGG TGACGCTGTC CTTCCTGCTC ATGCTGATCT TCATGACATA CAACGTGTGG 
CTCTGCCTGA TGGTTGTCCT GGGCGCCGCC GTTGGTTACT TCCTCTTCTG CTGGAAGAAA 
TCGGTTATCG TGGACGTAAC CGAGCACTGT CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572336.2
CDS	201..896
Translation	MDHAHHSAPGVDHSMHHDHVGMHHDHSGIPAATASPMDAASMFDLIPDTSDLQASHAGHAAHGAHNHGGGSGTGMEHMMPMAFHFGYNETILFSWWHIETVAGLIGSMIAIFLLALMYEGLKYYREYLFWKTYNLLEYRPVTGPQRNPEAPRIPSPAAAAPSPVQPSMLSINHLLQTLLHVLQVTLSFLLMLIFMTYNVWLCLMVVLGAAVGYFLFCWKKSVIVDVTEHCH

Target ORF information:

RefSeq Version	NM_132108.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster copper transporter 1A, transcript variant A (Ctr1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132108.3

ORF Insert Sequence:

ATGGACCACG CCCATCACAG TGCGCCCGGT GTTGATCACT CGATGCATCA CGATCATGTG 
GGCATGCACC ACGATCACAG CGGTATACCT GCTGCGACAG CGTCTCCAAT GGATGCAGCT 
TCCATGTTCG ACCTGATCCC CGATACCAGC GATTTGCAGG CAAGTCACGC GGGTCACGCC 
GCTCACGGAG CGCACAATCA TGGCGGTGGC TCTGGCACGG GAATGGAGCA CATGATGCCC 
ATGGCGTTCC ATTTTGGCTA CAACGAGACG ATCCTGTTCT CCTGGTGGCA CATCGAAACA 
GTGGCCGGTT TGATCGGCTC CATGATCGCC ATCTTCCTGC TGGCCCTAAT GTACGAGGGA 
CTGAAGTACT ACCGCGAGTA TCTATTCTGG AAGACATACA ATCTGCTGGA ATATCGACCT 
GTGACGGGGC CACAAAGAAA TCCGGAGGCA CCGCGCATAC CATCGCCGGC AGCGGCAGCT 
CCATCGCCCG TTCAACCTTC GATGCTGTCG ATTAACCACC TGCTCCAGAC ACTGCTGCAC 
GTGCTCCAGG TGACGCTGTC CTTCCTGCTC ATGCTGATCT TCATGACATA CAACGTGTGG 
CTCTGCCTGA TGGTTGTCCT GGGCGCCGCC GTTGGTTACT TCCTCTTCTG CTGGAAGAAA 
TCGGTTATCG TGGACGTAAC CGAGCACTGT CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctr1A ( NM_001258622.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245551.1 Drosophila melanogaster copper transporter 1A, transcript variant B (Ctr1A), mRNA.

Price & Availability↑

CloneID	OFa00956
Clone ID Related Accession (Same CDS sequence)	NM_132108.3 , NM_001258622.2
Accession Version	NM_001258622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	copper transporter 1A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258622.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCACG CCCATCACAG TGCGCCCGGT GTTGATCACT CGATGCATCA CGATCATGTG 
GGCATGCACC ACGATCACAG CGGTATACCT GCTGCGACAG CGTCTCCAAT GGATGCAGCT 
TCCATGTTCG ACCTGATCCC CGATACCAGC GATTTGCAGG CAAGTCACGC GGGTCACGCC 
GCTCACGGAG CGCACAATCA TGGCGGTGGC TCTGGCACGG GAATGGAGCA CATGATGCCC 
ATGGCGTTCC ATTTTGGCTA CAACGAGACG ATCCTGTTCT CCTGGTGGCA CATCGAAACA 
GTGGCCGGTT TGATCGGCTC CATGATCGCC ATCTTCCTGC TGGCCCTAAT GTACGAGGGA 
CTGAAGTACT ACCGCGAGTA TCTATTCTGG AAGACATACA ATCTGCTGGA ATATCGACCT 
GTGACGGGGC CACAAAGAAA TCCGGAGGCA CCGCGCATAC CATCGCCGGC AGCGGCAGCT 
CCATCGCCCG TTCAACCTTC GATGCTGTCG ATTAACCACC TGCTCCAGAC ACTGCTGCAC 
GTGCTCCAGG TGACGCTGTC CTTCCTGCTC ATGCTGATCT TCATGACATA CAACGTGTGG 
CTCTGCCTGA TGGTTGTCCT GGGCGCCGCC GTTGGTTACT TCCTCTTCTG CTGGAAGAAA 
TCGGTTATCG TGGACGTAAC CGAGCACTGT CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245551.1
CDS	303..998
Translation	MDHAHHSAPGVDHSMHHDHVGMHHDHSGIPAATASPMDAASMFDLIPDTSDLQASHAGHAAHGAHNHGGGSGTGMEHMMPMAFHFGYNETILFSWWHIETVAGLIGSMIAIFLLALMYEGLKYYREYLFWKTYNLLEYRPVTGPQRNPEAPRIPSPAAAAPSPVQPSMLSINHLLQTLLHVLQVTLSFLLMLIFMTYNVWLCLMVVLGAAVGYFLFCWKKSVIVDVTEHCH

Target ORF information:

RefSeq Version	NM_001258622.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster copper transporter 1A, transcript variant B (Ctr1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258622.2

ORF Insert Sequence:

ATGGACCACG CCCATCACAG TGCGCCCGGT GTTGATCACT CGATGCATCA CGATCATGTG 
GGCATGCACC ACGATCACAG CGGTATACCT GCTGCGACAG CGTCTCCAAT GGATGCAGCT 
TCCATGTTCG ACCTGATCCC CGATACCAGC GATTTGCAGG CAAGTCACGC GGGTCACGCC 
GCTCACGGAG CGCACAATCA TGGCGGTGGC TCTGGCACGG GAATGGAGCA CATGATGCCC 
ATGGCGTTCC ATTTTGGCTA CAACGAGACG ATCCTGTTCT CCTGGTGGCA CATCGAAACA 
GTGGCCGGTT TGATCGGCTC CATGATCGCC ATCTTCCTGC TGGCCCTAAT GTACGAGGGA 
CTGAAGTACT ACCGCGAGTA TCTATTCTGG AAGACATACA ATCTGCTGGA ATATCGACCT 
GTGACGGGGC CACAAAGAAA TCCGGAGGCA CCGCGCATAC CATCGCCGGC AGCGGCAGCT 
CCATCGCCCG TTCAACCTTC GATGCTGTCG ATTAACCACC TGCTCCAGAC ACTGCTGCAC 
GTGCTCCAGG TGACGCTGTC CTTCCTGCTC ATGCTGATCT TCATGACATA CAACGTGTGG 
CTCTGCCTGA TGGTTGTCCT GGGCGCCGCC GTTGGTTACT TCCTCTTCTG CTGGAAGAAA 
TCGGTTATCG TGGACGTAAC CGAGCACTGT CACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ctr1A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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