Nep1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nep1
Entrez Gene ID	31547
Full Name	Neprilysin 1
Synonyms	CG5894,CG5905,CG5914,DmeNEP1,Dmel\CG5905,NEP,NEP1,dNEP1,nep1
General protein information	Preferred Names Neprilysin 1 Names CG5905 gene product from transcript CG5905-RD CG5905-PA CG5905-PB CG5905-PC CG5905-PD Nep1-PA Nep1-PB Nep1-PC Nep1-PD neprilysin 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5D4-5D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_167059.2	NP_727065.1	neprilysin 1, isoform B
NM_001297996.1	NP_001284925.1	neprilysin 1, isoform C
NM_001297997.1	NP_001284926.1	neprilysin 1, isoform D
NM_078501.3	NP_511056.2	neprilysin 1, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ECE2	XP_005639826.1
Mus musculus (house mouse)	Ece2	NP_808810.1
Homo sapiens (human)	ECE2	NP_055508.3
	ECE2	XP_001098704.2
Drosophila melanogaster (fruit fly)	Nep1	NP_727065.1
Magnaporthe oryzae (rice blast fungus)	MGG_06643	XP_003709345.1
Bos taurus (cattle)	ECE2	NP_808871.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009791	XP_553645.3
Caenorhabditis elegans (roundworm)	nep-11	NP_494538.1
Rattus norvegicus (Norway rat)	Ece2	NP_001002815.2
Gallus gallus (chicken)	ECE2	XP_004943462.1
Pan troglodytes (chimpanzee)	ECE2	XP_001147750.1
Caenorhabditis elegans (roundworm)	nep-1	NP_496490.2
Xenopus tropicalis (tropical clawed frog)	ece2	XP_002935189.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The NEP1 specifically involved in middle-term memory (MTM) and long-term memory (LTM) and that their expression is required in the mushroom bodies and also in a single pair of closely connected neurons.
Title: Drosophila Neprilysins Are Involved in Middle-Term and Long-Term Memory.

The following Nep1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nep1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00933	NM_001297996.1 Latest version!	Drosophila melanogaster neprilysin 1, transcript variant C (Nep1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00933	NM_167059.2 Latest version!	Drosophila melanogaster neprilysin 1, transcript variant B (Nep1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00933	NM_078501.3 Latest version!	Drosophila melanogaster neprilysin 1, transcript variant A (Nep1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00933	NM_001297997.1 Latest version!	Drosophila melanogaster neprilysin 1, transcript variant D (Nep1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nep1 ( NM_167059.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727065.1 Drosophila melanogaster neprilysin 1, transcript variant B (Nep1), mRNA.

Price & Availability↑

CloneID	OFa00933
Clone ID Related Accession (Same CDS sequence)	NM_167059.2 , NM_001297996.1 , NM_001297997.1 , NM_078501.3
Accession Version	NM_167059.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neprilysin 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167059.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCAGC AACATGAGGC AACAGCGGCG GCCGCGGAGA AGCCGCTAAA TAACGGCTAC 
CTGCAGGCGA ATGCGCCGCT GGAGGAGCTG AGTGCCACGG TGGTGTCGCC ACTCCTGGGA 
CAGCAACAGG TGCAGCACCA GGCGCCACAT CAGATGCAGC AGCAACAGCA GCAGCAGCAG 
CAGAACAAGC TGCCCACTGT CGTTTTCCTG GCGCCCGACG GCAGCGGAGG CGTGGGAATC 
CAGCGAGGAA ATCCAGCGCA GGGCAATCCC GGCATGGTAA CGGGCACCGG CAGCCACAGC 
GACTGGCTGC TCAAGGAGTC GCAGCAGCGG CGCCGCCTGC TCGTCCTGGC CATCGCCTTT 
ACCGTTCTGG GCGCGGCCAT CGGGGCACTG GCCATCTACT TCGCCAGCGT CCATCAGCGT 
TGCCATCTGT ATCGCCTGGA GCCAGACAAC GATGACAGAC CGAACGGCAG GTGGAACCAG 
GATTCGGGAT CCGCCCACGA GGGGCAGGAT AACATTTGCA TGACCCAGGA GTGCGTGAGA 
ACAGCCGCCT CCCTGCTCTC GGCCATGGAC CTCAACTCCG ATCCCTGCGA GGACTTTTTC 
CAGTACGCGT GCGGCACGTG GAACAAGATG CATCCCATTC CCGAGGACCG CAGCTCCATC 
AGCACGTTTG AGGTCCTGTC CGATCAGCAG CAGGTTATCC TGCGCGCAGT TCTGGAGGAA 
CCCATCGATG AACGCGACAA CAAAGCCACC ATAAAGGCCA AAACCTTCTT CAAGAGCTGC 
ATGGATATTC CTCAAATCCG CAAAATCGGA ACGGGTCGAC TCAAGCAGGT ACTGCAATCC 
CTGGGCGGCT GGCCGGTCAT CGAGCGGAAC TGGAGTCCGC CGGCGGATCT CTCCGTGGAG 
CGGCTAATGG GTCAGCTCCG ACTGAACTAC AGCGAGCCGG TGATGATCGA GCTGTACGTG 
GGCGCCGACG ACAAGAACAG CTCGGTGAAC ATCCTGCAGA TGGACCAGCT GCAGTATGCC 
CTGCCCTCGA GGGACTACTA CCTGAAGGAG AGCAGTGCGA ACGACCGAAG GGCCTACCAT 
CGGTATATGA CACAGGTGGC GCTGCTCCTG GGAGCGGATC CGGCCACCGC CGCCGCGGAA 
CTGGAGAAAG TTGTGCTCTT CGAGACGCAG CTAGTGAATG TTTCCTTGCC GGAGGCGGAT 
CGTCATGACA CTAGCCTGGT CTATCGTAAG ATGTTGCTGC CCGAGCTGCA GGAACTGGTG 
CCCGAGGTGC AGTGGCAGGA GTATCTACAG GCAGCTCTGG GTCCGGGGAT TCCCCTGCAG 
GAGGACGAGC CGCTGGTCAC CTACGGGCTG CACTATCTCA CCGAAATGGG CAAGATACTG 
GCGCACACCG ATCGCCGTGT GGTGCACAAC TATATGCTGT GGCGCCTGGT CATGTCCTTG 
ATGTCACACA TGATCGATGA GTATCAGCGT GAGCGGGTGG AGTTCCGCAA GATCCTAATG 
GGCATCCAGT CGGAGAGGAC ACGCTGGTCA CAGTGCGTCG AGTGGACTAA CAAGAAGCTG 
GGTGTCGCCG TGGGAGCCCT CTTCATCCGG GACAACTTCA ATCAGGAGAG CAAGGAGGTG 
GCCCTCGAGA TGATCCACAC TATACGTGCG GCGTTCAACG AGCTTCTGGC CGAGAACGAT 
TGGATGGACG ACGAGACGCG GGCGGTGGCC AAGGAGAAGG CGGACTCGAT GAACGAGCGG 
ATTGGTTATC CGGAGCTGCT CACCAATGCC ACCGAGCTGG AGCAGGAGTA CGTGAATCTA 
ACAATTGTCC CCGACAACTT CATCAACAAT GTCCTTAGCA TTCTGCAATG GGAGTCGGAG 
AAGATGCTGC GACTTCTTCG CCAGCCGGTG GACAAGGAAA AGTGGACCAC CGAACCGGCG 
GTGGTGAATG CCTTCTACAA CCCGAATAAA AACGATATAG TATTTCCTGC TGGCATCCTG 
CAGCCGCTAT TCTACAGCCA GCACTTTCCC AAGTCCTTGA ATTACGGCGG AATCGGAGTG 
GTCATTGGCC ACGAGATTAC CCACGGATTC GACGACAAGG GCAGGCAGTT CGACAAGGAG 
GGCAACATGA TGCAGTGGTG GAACAATGCC ACCATCGAGG CCTTTCGCGA ACGGACGCAG 
TGCGTCATCG ATCAGTACTC GCGCTACAAG ATTAACGAGG TGGACATGTT CATGGACGGA 
CGGATGACGC AGGGCGAGAA CATCGCCGAC AACGGCGGTC TAAAACAGGC TTTCAGGGCC 
TACAAGAAAT GGGAGACCTT ACATGGGCGG GAGCAGCAGC TGCCCGGCCT GAACATGACC 
CACGATCAGC TGTTCTTTCT CAACTACGCC CAAATCTGGT GCGGATCCAT GCGGCCGGAG 
GACGCTCTGA CCAAGATCCG CTCCGCGGTC CACTCGCCCG GATTCGTTCG TGTCCTGGGA 
CCACTTTCGA ATTCTCGCGA CTTTGCCAGC GCCTACAAAT GCCCACTGGG CAGCACCATG 
AATCCGGCGG AAAAGTGCAG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727065.1
CDS	468..3017
Translation	MSQQHEATAAAAEKPLNNGYLQANAPLEELSATVVSPLLGQQQVQHQAPHQMQQQQQQQQQNKLPTVVFLAPDGSGGVGIQRGNPAQGNPGMVTGTGSHSDWLLKESQQRRRLLVLAIAFTVLGAAIGALAIYFASVHQRCHLYRLEPDNDDRPNGRWNQDSGSAHEGQDNICMTQECVRTAASLLSAMDLNSDPCEDFFQYACGTWNKMHPIPEDRSSISTFEVLSDQQQVILRAVLEEPIDERDNKATIKAKTFFKSCMDIPQIRKIGTGRLKQVLQSLGGWPVIERNWSPPADLSVERLMGQLRLNYSEPVMIELYVGADDKNSSVNILQMDQLQYALPSRDYYLKESSANDRRAYHRYMTQVALLLGADPATAAAELEKVVLFETQLVNVSLPEADRHDTSLVYRKMLLPELQELVPEVQWQEYLQAALGPGIPLQEDEPLVTYGLHYLTEMGKILAHTDRRVVHNYMLWRLVMSLMSHMIDEYQRERVEFRKILMGIQSERTRWSQCVEWTNKKLGVAVGALFIRDNFNQESKEVALEMIHTIRAAFNELLAENDWMDDETRAVAKEKADSMNERIGYPELLTNATELEQEYVNLTIVPDNFINNVLSILQWESEKMLRLLRQPVDKEKWTTEPAVVNAFYNPNKNDIVFPAGILQPLFYSQHFPKSLNYGGIGVVIGHEITHGFDDKGRQFDKEGNMMQWWNNATIEAFRERTQCVIDQYSRYKINEVDMFMDGRMTQGENIADNGGLKQAFRAYKKWETLHGREQQLPGLNMTHDQLFFLNYAQIWCGSMRPEDALTKIRSAVHSPGFVRVLGPLSNSRDFASAYKCPLGSTMNPAEKCSVW

Target ORF information:

RefSeq Version	NM_167059.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neprilysin 1, transcript variant B (Nep1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167059.2

ORF Insert Sequence:

ATGTCGCAGC AACATGAGGC AACAGCGGCG GCCGCGGAGA AGCCGCTAAA TAACGGCTAC 
CTGCAGGCGA ATGCGCCGCT GGAGGAGCTG AGTGCCACGG TGGTGTCGCC ACTCCTGGGA 
CAGCAACAGG TGCAGCACCA GGCGCCACAT CAGATGCAGC AGCAACAGCA GCAGCAGCAG 
CAGAACAAGC TGCCCACTGT CGTTTTCCTG GCGCCCGACG GCAGCGGAGG CGTGGGAATC 
CAGCGAGGAA ATCCAGCGCA GGGCAATCCC GGCATGGTAA CGGGCACCGG CAGCCACAGC 
GACTGGCTGC TCAAGGAGTC GCAGCAGCGG CGCCGCCTGC TCGTCCTGGC CATCGCCTTT 
ACCGTTCTGG GCGCGGCCAT CGGGGCACTG GCCATCTACT TCGCCAGCGT CCATCAGCGT 
TGCCATCTGT ATCGCCTGGA GCCAGACAAC GATGACAGAC CGAACGGCAG GTGGAACCAG 
GATTCGGGAT CCGCCCACGA GGGGCAGGAT AACATTTGCA TGACCCAGGA GTGCGTGAGA 
ACAGCCGCCT CCCTGCTCTC GGCCATGGAC CTCAACTCCG ATCCCTGCGA GGACTTTTTC 
CAGTACGCGT GCGGCACGTG GAACAAGATG CATCCCATTC CCGAGGACCG CAGCTCCATC 
AGCACGTTTG AGGTCCTGTC CGATCAGCAG CAGGTTATCC TGCGCGCAGT TCTGGAGGAA 
CCCATCGATG AACGCGACAA CAAAGCCACC ATAAAGGCCA AAACCTTCTT CAAGAGCTGC 
ATGGATATTC CTCAAATCCG CAAAATCGGA ACGGGTCGAC TCAAGCAGGT ACTGCAATCC 
CTGGGCGGCT GGCCGGTCAT CGAGCGGAAC TGGAGTCCGC CGGCGGATCT CTCCGTGGAG 
CGGCTAATGG GTCAGCTCCG ACTGAACTAC AGCGAGCCGG TGATGATCGA GCTGTACGTG 
GGCGCCGACG ACAAGAACAG CTCGGTGAAC ATCCTGCAGA TGGACCAGCT GCAGTATGCC 
CTGCCCTCGA GGGACTACTA CCTGAAGGAG AGCAGTGCGA ACGACCGAAG GGCCTACCAT 
CGGTATATGA CACAGGTGGC GCTGCTCCTG GGAGCGGATC CGGCCACCGC CGCCGCGGAA 
CTGGAGAAAG TTGTGCTCTT CGAGACGCAG CTAGTGAATG TTTCCTTGCC GGAGGCGGAT 
CGTCATGACA CTAGCCTGGT CTATCGTAAG ATGTTGCTGC CCGAGCTGCA GGAACTGGTG 
CCCGAGGTGC AGTGGCAGGA GTATCTACAG GCAGCTCTGG GTCCGGGGAT TCCCCTGCAG 
GAGGACGAGC CGCTGGTCAC CTACGGGCTG CACTATCTCA CCGAAATGGG CAAGATACTG 
GCGCACACCG ATCGCCGTGT GGTGCACAAC TATATGCTGT GGCGCCTGGT CATGTCCTTG 
ATGTCACACA TGATCGATGA GTATCAGCGT GAGCGGGTGG AGTTCCGCAA GATCCTAATG 
GGCATCCAGT CGGAGAGGAC ACGCTGGTCA CAGTGCGTCG AGTGGACTAA CAAGAAGCTG 
GGTGTCGCCG TGGGAGCCCT CTTCATCCGG GACAACTTCA ATCAGGAGAG CAAGGAGGTG 
GCCCTCGAGA TGATCCACAC TATACGTGCG GCGTTCAACG AGCTTCTGGC CGAGAACGAT 
TGGATGGACG ACGAGACGCG GGCGGTGGCC AAGGAGAAGG CGGACTCGAT GAACGAGCGG 
ATTGGTTATC CGGAGCTGCT CACCAATGCC ACCGAGCTGG AGCAGGAGTA CGTGAATCTA 
ACAATTGTCC CCGACAACTT CATCAACAAT GTCCTTAGCA TTCTGCAATG GGAGTCGGAG 
AAGATGCTGC GACTTCTTCG CCAGCCGGTG GACAAGGAAA AGTGGACCAC CGAACCGGCG 
GTGGTGAATG CCTTCTACAA CCCGAATAAA AACGATATAG TATTTCCTGC TGGCATCCTG 
CAGCCGCTAT TCTACAGCCA GCACTTTCCC AAGTCCTTGA ATTACGGCGG AATCGGAGTG 
GTCATTGGCC ACGAGATTAC CCACGGATTC GACGACAAGG GCAGGCAGTT CGACAAGGAG 
GGCAACATGA TGCAGTGGTG GAACAATGCC ACCATCGAGG CCTTTCGCGA ACGGACGCAG 
TGCGTCATCG ATCAGTACTC GCGCTACAAG ATTAACGAGG TGGACATGTT CATGGACGGA 
CGGATGACGC AGGGCGAGAA CATCGCCGAC AACGGCGGTC TAAAACAGGC TTTCAGGGCC 
TACAAGAAAT GGGAGACCTT ACATGGGCGG GAGCAGCAGC TGCCCGGCCT GAACATGACC 
CACGATCAGC TGTTCTTTCT CAACTACGCC CAAATCTGGT GCGGATCCAT GCGGCCGGAG 
GACGCTCTGA CCAAGATCCG CTCCGCGGTC CACTCGCCCG GATTCGTTCG TGTCCTGGGA 
CCACTTTCGA ATTCTCGCGA CTTTGCCAGC GCCTACAAAT GCCCACTGGG CAGCACCATG 
AATCCGGCGG AAAAGTGCAG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nep1 ( NM_001297996.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284925.1 Drosophila melanogaster neprilysin 1, transcript variant C (Nep1), mRNA.

Price & Availability↑

CloneID	OFa00933
Clone ID Related Accession (Same CDS sequence)	NM_167059.2 , NM_001297996.1 , NM_001297997.1 , NM_078501.3
Accession Version	NM_001297996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neprilysin 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCAGC AACATGAGGC AACAGCGGCG GCCGCGGAGA AGCCGCTAAA TAACGGCTAC 
CTGCAGGCGA ATGCGCCGCT GGAGGAGCTG AGTGCCACGG TGGTGTCGCC ACTCCTGGGA 
CAGCAACAGG TGCAGCACCA GGCGCCACAT CAGATGCAGC AGCAACAGCA GCAGCAGCAG 
CAGAACAAGC TGCCCACTGT CGTTTTCCTG GCGCCCGACG GCAGCGGAGG CGTGGGAATC 
CAGCGAGGAA ATCCAGCGCA GGGCAATCCC GGCATGGTAA CGGGCACCGG CAGCCACAGC 
GACTGGCTGC TCAAGGAGTC GCAGCAGCGG CGCCGCCTGC TCGTCCTGGC CATCGCCTTT 
ACCGTTCTGG GCGCGGCCAT CGGGGCACTG GCCATCTACT TCGCCAGCGT CCATCAGCGT 
TGCCATCTGT ATCGCCTGGA GCCAGACAAC GATGACAGAC CGAACGGCAG GTGGAACCAG 
GATTCGGGAT CCGCCCACGA GGGGCAGGAT AACATTTGCA TGACCCAGGA GTGCGTGAGA 
ACAGCCGCCT CCCTGCTCTC GGCCATGGAC CTCAACTCCG ATCCCTGCGA GGACTTTTTC 
CAGTACGCGT GCGGCACGTG GAACAAGATG CATCCCATTC CCGAGGACCG CAGCTCCATC 
AGCACGTTTG AGGTCCTGTC CGATCAGCAG CAGGTTATCC TGCGCGCAGT TCTGGAGGAA 
CCCATCGATG AACGCGACAA CAAAGCCACC ATAAAGGCCA AAACCTTCTT CAAGAGCTGC 
ATGGATATTC CTCAAATCCG CAAAATCGGA ACGGGTCGAC TCAAGCAGGT ACTGCAATCC 
CTGGGCGGCT GGCCGGTCAT CGAGCGGAAC TGGAGTCCGC CGGCGGATCT CTCCGTGGAG 
CGGCTAATGG GTCAGCTCCG ACTGAACTAC AGCGAGCCGG TGATGATCGA GCTGTACGTG 
GGCGCCGACG ACAAGAACAG CTCGGTGAAC ATCCTGCAGA TGGACCAGCT GCAGTATGCC 
CTGCCCTCGA GGGACTACTA CCTGAAGGAG AGCAGTGCGA ACGACCGAAG GGCCTACCAT 
CGGTATATGA CACAGGTGGC GCTGCTCCTG GGAGCGGATC CGGCCACCGC CGCCGCGGAA 
CTGGAGAAAG TTGTGCTCTT CGAGACGCAG CTAGTGAATG TTTCCTTGCC GGAGGCGGAT 
CGTCATGACA CTAGCCTGGT CTATCGTAAG ATGTTGCTGC CCGAGCTGCA GGAACTGGTG 
CCCGAGGTGC AGTGGCAGGA GTATCTACAG GCAGCTCTGG GTCCGGGGAT TCCCCTGCAG 
GAGGACGAGC CGCTGGTCAC CTACGGGCTG CACTATCTCA CCGAAATGGG CAAGATACTG 
GCGCACACCG ATCGCCGTGT GGTGCACAAC TATATGCTGT GGCGCCTGGT CATGTCCTTG 
ATGTCACACA TGATCGATGA GTATCAGCGT GAGCGGGTGG AGTTCCGCAA GATCCTAATG 
GGCATCCAGT CGGAGAGGAC ACGCTGGTCA CAGTGCGTCG AGTGGACTAA CAAGAAGCTG 
GGTGTCGCCG TGGGAGCCCT CTTCATCCGG GACAACTTCA ATCAGGAGAG CAAGGAGGTG 
GCCCTCGAGA TGATCCACAC TATACGTGCG GCGTTCAACG AGCTTCTGGC CGAGAACGAT 
TGGATGGACG ACGAGACGCG GGCGGTGGCC AAGGAGAAGG CGGACTCGAT GAACGAGCGG 
ATTGGTTATC CGGAGCTGCT CACCAATGCC ACCGAGCTGG AGCAGGAGTA CGTGAATCTA 
ACAATTGTCC CCGACAACTT CATCAACAAT GTCCTTAGCA TTCTGCAATG GGAGTCGGAG 
AAGATGCTGC GACTTCTTCG CCAGCCGGTG GACAAGGAAA AGTGGACCAC CGAACCGGCG 
GTGGTGAATG CCTTCTACAA CCCGAATAAA AACGATATAG TATTTCCTGC TGGCATCCTG 
CAGCCGCTAT TCTACAGCCA GCACTTTCCC AAGTCCTTGA ATTACGGCGG AATCGGAGTG 
GTCATTGGCC ACGAGATTAC CCACGGATTC GACGACAAGG GCAGGCAGTT CGACAAGGAG 
GGCAACATGA TGCAGTGGTG GAACAATGCC ACCATCGAGG CCTTTCGCGA ACGGACGCAG 
TGCGTCATCG ATCAGTACTC GCGCTACAAG ATTAACGAGG TGGACATGTT CATGGACGGA 
CGGATGACGC AGGGCGAGAA CATCGCCGAC AACGGCGGTC TAAAACAGGC TTTCAGGGCC 
TACAAGAAAT GGGAGACCTT ACATGGGCGG GAGCAGCAGC TGCCCGGCCT GAACATGACC 
CACGATCAGC TGTTCTTTCT CAACTACGCC CAAATCTGGT GCGGATCCAT GCGGCCGGAG 
GACGCTCTGA CCAAGATCCG CTCCGCGGTC CACTCGCCCG GATTCGTTCG TGTCCTGGGA 
CCACTTTCGA ATTCTCGCGA CTTTGCCAGC GCCTACAAAT GCCCACTGGG CAGCACCATG 
AATCCGGCGG AAAAGTGCAG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284925.1
CDS	636..3185
Translation	MSQQHEATAAAAEKPLNNGYLQANAPLEELSATVVSPLLGQQQVQHQAPHQMQQQQQQQQQNKLPTVVFLAPDGSGGVGIQRGNPAQGNPGMVTGTGSHSDWLLKESQQRRRLLVLAIAFTVLGAAIGALAIYFASVHQRCHLYRLEPDNDDRPNGRWNQDSGSAHEGQDNICMTQECVRTAASLLSAMDLNSDPCEDFFQYACGTWNKMHPIPEDRSSISTFEVLSDQQQVILRAVLEEPIDERDNKATIKAKTFFKSCMDIPQIRKIGTGRLKQVLQSLGGWPVIERNWSPPADLSVERLMGQLRLNYSEPVMIELYVGADDKNSSVNILQMDQLQYALPSRDYYLKESSANDRRAYHRYMTQVALLLGADPATAAAELEKVVLFETQLVNVSLPEADRHDTSLVYRKMLLPELQELVPEVQWQEYLQAALGPGIPLQEDEPLVTYGLHYLTEMGKILAHTDRRVVHNYMLWRLVMSLMSHMIDEYQRERVEFRKILMGIQSERTRWSQCVEWTNKKLGVAVGALFIRDNFNQESKEVALEMIHTIRAAFNELLAENDWMDDETRAVAKEKADSMNERIGYPELLTNATELEQEYVNLTIVPDNFINNVLSILQWESEKMLRLLRQPVDKEKWTTEPAVVNAFYNPNKNDIVFPAGILQPLFYSQHFPKSLNYGGIGVVIGHEITHGFDDKGRQFDKEGNMMQWWNNATIEAFRERTQCVIDQYSRYKINEVDMFMDGRMTQGENIADNGGLKQAFRAYKKWETLHGREQQLPGLNMTHDQLFFLNYAQIWCGSMRPEDALTKIRSAVHSPGFVRVLGPLSNSRDFASAYKCPLGSTMNPAEKCSVW

Target ORF information:

RefSeq Version	NM_001297996.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neprilysin 1, transcript variant C (Nep1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297996.1

ORF Insert Sequence:

ATGTCGCAGC AACATGAGGC AACAGCGGCG GCCGCGGAGA AGCCGCTAAA TAACGGCTAC 
CTGCAGGCGA ATGCGCCGCT GGAGGAGCTG AGTGCCACGG TGGTGTCGCC ACTCCTGGGA 
CAGCAACAGG TGCAGCACCA GGCGCCACAT CAGATGCAGC AGCAACAGCA GCAGCAGCAG 
CAGAACAAGC TGCCCACTGT CGTTTTCCTG GCGCCCGACG GCAGCGGAGG CGTGGGAATC 
CAGCGAGGAA ATCCAGCGCA GGGCAATCCC GGCATGGTAA CGGGCACCGG CAGCCACAGC 
GACTGGCTGC TCAAGGAGTC GCAGCAGCGG CGCCGCCTGC TCGTCCTGGC CATCGCCTTT 
ACCGTTCTGG GCGCGGCCAT CGGGGCACTG GCCATCTACT TCGCCAGCGT CCATCAGCGT 
TGCCATCTGT ATCGCCTGGA GCCAGACAAC GATGACAGAC CGAACGGCAG GTGGAACCAG 
GATTCGGGAT CCGCCCACGA GGGGCAGGAT AACATTTGCA TGACCCAGGA GTGCGTGAGA 
ACAGCCGCCT CCCTGCTCTC GGCCATGGAC CTCAACTCCG ATCCCTGCGA GGACTTTTTC 
CAGTACGCGT GCGGCACGTG GAACAAGATG CATCCCATTC CCGAGGACCG CAGCTCCATC 
AGCACGTTTG AGGTCCTGTC CGATCAGCAG CAGGTTATCC TGCGCGCAGT TCTGGAGGAA 
CCCATCGATG AACGCGACAA CAAAGCCACC ATAAAGGCCA AAACCTTCTT CAAGAGCTGC 
ATGGATATTC CTCAAATCCG CAAAATCGGA ACGGGTCGAC TCAAGCAGGT ACTGCAATCC 
CTGGGCGGCT GGCCGGTCAT CGAGCGGAAC TGGAGTCCGC CGGCGGATCT CTCCGTGGAG 
CGGCTAATGG GTCAGCTCCG ACTGAACTAC AGCGAGCCGG TGATGATCGA GCTGTACGTG 
GGCGCCGACG ACAAGAACAG CTCGGTGAAC ATCCTGCAGA TGGACCAGCT GCAGTATGCC 
CTGCCCTCGA GGGACTACTA CCTGAAGGAG AGCAGTGCGA ACGACCGAAG GGCCTACCAT 
CGGTATATGA CACAGGTGGC GCTGCTCCTG GGAGCGGATC CGGCCACCGC CGCCGCGGAA 
CTGGAGAAAG TTGTGCTCTT CGAGACGCAG CTAGTGAATG TTTCCTTGCC GGAGGCGGAT 
CGTCATGACA CTAGCCTGGT CTATCGTAAG ATGTTGCTGC CCGAGCTGCA GGAACTGGTG 
CCCGAGGTGC AGTGGCAGGA GTATCTACAG GCAGCTCTGG GTCCGGGGAT TCCCCTGCAG 
GAGGACGAGC CGCTGGTCAC CTACGGGCTG CACTATCTCA CCGAAATGGG CAAGATACTG 
GCGCACACCG ATCGCCGTGT GGTGCACAAC TATATGCTGT GGCGCCTGGT CATGTCCTTG 
ATGTCACACA TGATCGATGA GTATCAGCGT GAGCGGGTGG AGTTCCGCAA GATCCTAATG 
GGCATCCAGT CGGAGAGGAC ACGCTGGTCA CAGTGCGTCG AGTGGACTAA CAAGAAGCTG 
GGTGTCGCCG TGGGAGCCCT CTTCATCCGG GACAACTTCA ATCAGGAGAG CAAGGAGGTG 
GCCCTCGAGA TGATCCACAC TATACGTGCG GCGTTCAACG AGCTTCTGGC CGAGAACGAT 
TGGATGGACG ACGAGACGCG GGCGGTGGCC AAGGAGAAGG CGGACTCGAT GAACGAGCGG 
ATTGGTTATC CGGAGCTGCT CACCAATGCC ACCGAGCTGG AGCAGGAGTA CGTGAATCTA 
ACAATTGTCC CCGACAACTT CATCAACAAT GTCCTTAGCA TTCTGCAATG GGAGTCGGAG 
AAGATGCTGC GACTTCTTCG CCAGCCGGTG GACAAGGAAA AGTGGACCAC CGAACCGGCG 
GTGGTGAATG CCTTCTACAA CCCGAATAAA AACGATATAG TATTTCCTGC TGGCATCCTG 
CAGCCGCTAT TCTACAGCCA GCACTTTCCC AAGTCCTTGA ATTACGGCGG AATCGGAGTG 
GTCATTGGCC ACGAGATTAC CCACGGATTC GACGACAAGG GCAGGCAGTT CGACAAGGAG 
GGCAACATGA TGCAGTGGTG GAACAATGCC ACCATCGAGG CCTTTCGCGA ACGGACGCAG 
TGCGTCATCG ATCAGTACTC GCGCTACAAG ATTAACGAGG TGGACATGTT CATGGACGGA 
CGGATGACGC AGGGCGAGAA CATCGCCGAC AACGGCGGTC TAAAACAGGC TTTCAGGGCC 
TACAAGAAAT GGGAGACCTT ACATGGGCGG GAGCAGCAGC TGCCCGGCCT GAACATGACC 
CACGATCAGC TGTTCTTTCT CAACTACGCC CAAATCTGGT GCGGATCCAT GCGGCCGGAG 
GACGCTCTGA CCAAGATCCG CTCCGCGGTC CACTCGCCCG GATTCGTTCG TGTCCTGGGA 
CCACTTTCGA ATTCTCGCGA CTTTGCCAGC GCCTACAAAT GCCCACTGGG CAGCACCATG 
AATCCGGCGG AAAAGTGCAG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nep1 ( NM_001297997.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284926.1 Drosophila melanogaster neprilysin 1, transcript variant D (Nep1), mRNA.

Price & Availability↑

CloneID	OFa00933
Clone ID Related Accession (Same CDS sequence)	NM_167059.2 , NM_001297996.1 , NM_001297997.1 , NM_078501.3
Accession Version	NM_001297997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neprilysin 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCAGC AACATGAGGC AACAGCGGCG GCCGCGGAGA AGCCGCTAAA TAACGGCTAC 
CTGCAGGCGA ATGCGCCGCT GGAGGAGCTG AGTGCCACGG TGGTGTCGCC ACTCCTGGGA 
CAGCAACAGG TGCAGCACCA GGCGCCACAT CAGATGCAGC AGCAACAGCA GCAGCAGCAG 
CAGAACAAGC TGCCCACTGT CGTTTTCCTG GCGCCCGACG GCAGCGGAGG CGTGGGAATC 
CAGCGAGGAA ATCCAGCGCA GGGCAATCCC GGCATGGTAA CGGGCACCGG CAGCCACAGC 
GACTGGCTGC TCAAGGAGTC GCAGCAGCGG CGCCGCCTGC TCGTCCTGGC CATCGCCTTT 
ACCGTTCTGG GCGCGGCCAT CGGGGCACTG GCCATCTACT TCGCCAGCGT CCATCAGCGT 
TGCCATCTGT ATCGCCTGGA GCCAGACAAC GATGACAGAC CGAACGGCAG GTGGAACCAG 
GATTCGGGAT CCGCCCACGA GGGGCAGGAT AACATTTGCA TGACCCAGGA GTGCGTGAGA 
ACAGCCGCCT CCCTGCTCTC GGCCATGGAC CTCAACTCCG ATCCCTGCGA GGACTTTTTC 
CAGTACGCGT GCGGCACGTG GAACAAGATG CATCCCATTC CCGAGGACCG CAGCTCCATC 
AGCACGTTTG AGGTCCTGTC CGATCAGCAG CAGGTTATCC TGCGCGCAGT TCTGGAGGAA 
CCCATCGATG AACGCGACAA CAAAGCCACC ATAAAGGCCA AAACCTTCTT CAAGAGCTGC 
ATGGATATTC CTCAAATCCG CAAAATCGGA ACGGGTCGAC TCAAGCAGGT ACTGCAATCC 
CTGGGCGGCT GGCCGGTCAT CGAGCGGAAC TGGAGTCCGC CGGCGGATCT CTCCGTGGAG 
CGGCTAATGG GTCAGCTCCG ACTGAACTAC AGCGAGCCGG TGATGATCGA GCTGTACGTG 
GGCGCCGACG ACAAGAACAG CTCGGTGAAC ATCCTGCAGA TGGACCAGCT GCAGTATGCC 
CTGCCCTCGA GGGACTACTA CCTGAAGGAG AGCAGTGCGA ACGACCGAAG GGCCTACCAT 
CGGTATATGA CACAGGTGGC GCTGCTCCTG GGAGCGGATC CGGCCACCGC CGCCGCGGAA 
CTGGAGAAAG TTGTGCTCTT CGAGACGCAG CTAGTGAATG TTTCCTTGCC GGAGGCGGAT 
CGTCATGACA CTAGCCTGGT CTATCGTAAG ATGTTGCTGC CCGAGCTGCA GGAACTGGTG 
CCCGAGGTGC AGTGGCAGGA GTATCTACAG GCAGCTCTGG GTCCGGGGAT TCCCCTGCAG 
GAGGACGAGC CGCTGGTCAC CTACGGGCTG CACTATCTCA CCGAAATGGG CAAGATACTG 
GCGCACACCG ATCGCCGTGT GGTGCACAAC TATATGCTGT GGCGCCTGGT CATGTCCTTG 
ATGTCACACA TGATCGATGA GTATCAGCGT GAGCGGGTGG AGTTCCGCAA GATCCTAATG 
GGCATCCAGT CGGAGAGGAC ACGCTGGTCA CAGTGCGTCG AGTGGACTAA CAAGAAGCTG 
GGTGTCGCCG TGGGAGCCCT CTTCATCCGG GACAACTTCA ATCAGGAGAG CAAGGAGGTG 
GCCCTCGAGA TGATCCACAC TATACGTGCG GCGTTCAACG AGCTTCTGGC CGAGAACGAT 
TGGATGGACG ACGAGACGCG GGCGGTGGCC AAGGAGAAGG CGGACTCGAT GAACGAGCGG 
ATTGGTTATC CGGAGCTGCT CACCAATGCC ACCGAGCTGG AGCAGGAGTA CGTGAATCTA 
ACAATTGTCC CCGACAACTT CATCAACAAT GTCCTTAGCA TTCTGCAATG GGAGTCGGAG 
AAGATGCTGC GACTTCTTCG CCAGCCGGTG GACAAGGAAA AGTGGACCAC CGAACCGGCG 
GTGGTGAATG CCTTCTACAA CCCGAATAAA AACGATATAG TATTTCCTGC TGGCATCCTG 
CAGCCGCTAT TCTACAGCCA GCACTTTCCC AAGTCCTTGA ATTACGGCGG AATCGGAGTG 
GTCATTGGCC ACGAGATTAC CCACGGATTC GACGACAAGG GCAGGCAGTT CGACAAGGAG 
GGCAACATGA TGCAGTGGTG GAACAATGCC ACCATCGAGG CCTTTCGCGA ACGGACGCAG 
TGCGTCATCG ATCAGTACTC GCGCTACAAG ATTAACGAGG TGGACATGTT CATGGACGGA 
CGGATGACGC AGGGCGAGAA CATCGCCGAC AACGGCGGTC TAAAACAGGC TTTCAGGGCC 
TACAAGAAAT GGGAGACCTT ACATGGGCGG GAGCAGCAGC TGCCCGGCCT GAACATGACC 
CACGATCAGC TGTTCTTTCT CAACTACGCC CAAATCTGGT GCGGATCCAT GCGGCCGGAG 
GACGCTCTGA CCAAGATCCG CTCCGCGGTC CACTCGCCCG GATTCGTTCG TGTCCTGGGA 
CCACTTTCGA ATTCTCGCGA CTTTGCCAGC GCCTACAAAT GCCCACTGGG CAGCACCATG 
AATCCGGCGG AAAAGTGCAG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284926.1
CDS	1105..3654
Translation	MSQQHEATAAAAEKPLNNGYLQANAPLEELSATVVSPLLGQQQVQHQAPHQMQQQQQQQQQNKLPTVVFLAPDGSGGVGIQRGNPAQGNPGMVTGTGSHSDWLLKESQQRRRLLVLAIAFTVLGAAIGALAIYFASVHQRCHLYRLEPDNDDRPNGRWNQDSGSAHEGQDNICMTQECVRTAASLLSAMDLNSDPCEDFFQYACGTWNKMHPIPEDRSSISTFEVLSDQQQVILRAVLEEPIDERDNKATIKAKTFFKSCMDIPQIRKIGTGRLKQVLQSLGGWPVIERNWSPPADLSVERLMGQLRLNYSEPVMIELYVGADDKNSSVNILQMDQLQYALPSRDYYLKESSANDRRAYHRYMTQVALLLGADPATAAAELEKVVLFETQLVNVSLPEADRHDTSLVYRKMLLPELQELVPEVQWQEYLQAALGPGIPLQEDEPLVTYGLHYLTEMGKILAHTDRRVVHNYMLWRLVMSLMSHMIDEYQRERVEFRKILMGIQSERTRWSQCVEWTNKKLGVAVGALFIRDNFNQESKEVALEMIHTIRAAFNELLAENDWMDDETRAVAKEKADSMNERIGYPELLTNATELEQEYVNLTIVPDNFINNVLSILQWESEKMLRLLRQPVDKEKWTTEPAVVNAFYNPNKNDIVFPAGILQPLFYSQHFPKSLNYGGIGVVIGHEITHGFDDKGRQFDKEGNMMQWWNNATIEAFRERTQCVIDQYSRYKINEVDMFMDGRMTQGENIADNGGLKQAFRAYKKWETLHGREQQLPGLNMTHDQLFFLNYAQIWCGSMRPEDALTKIRSAVHSPGFVRVLGPLSNSRDFASAYKCPLGSTMNPAEKCSVW

Target ORF information:

RefSeq Version	NM_001297997.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neprilysin 1, transcript variant D (Nep1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297997.1

ORF Insert Sequence:

ATGTCGCAGC AACATGAGGC AACAGCGGCG GCCGCGGAGA AGCCGCTAAA TAACGGCTAC 
CTGCAGGCGA ATGCGCCGCT GGAGGAGCTG AGTGCCACGG TGGTGTCGCC ACTCCTGGGA 
CAGCAACAGG TGCAGCACCA GGCGCCACAT CAGATGCAGC AGCAACAGCA GCAGCAGCAG 
CAGAACAAGC TGCCCACTGT CGTTTTCCTG GCGCCCGACG GCAGCGGAGG CGTGGGAATC 
CAGCGAGGAA ATCCAGCGCA GGGCAATCCC GGCATGGTAA CGGGCACCGG CAGCCACAGC 
GACTGGCTGC TCAAGGAGTC GCAGCAGCGG CGCCGCCTGC TCGTCCTGGC CATCGCCTTT 
ACCGTTCTGG GCGCGGCCAT CGGGGCACTG GCCATCTACT TCGCCAGCGT CCATCAGCGT 
TGCCATCTGT ATCGCCTGGA GCCAGACAAC GATGACAGAC CGAACGGCAG GTGGAACCAG 
GATTCGGGAT CCGCCCACGA GGGGCAGGAT AACATTTGCA TGACCCAGGA GTGCGTGAGA 
ACAGCCGCCT CCCTGCTCTC GGCCATGGAC CTCAACTCCG ATCCCTGCGA GGACTTTTTC 
CAGTACGCGT GCGGCACGTG GAACAAGATG CATCCCATTC CCGAGGACCG CAGCTCCATC 
AGCACGTTTG AGGTCCTGTC CGATCAGCAG CAGGTTATCC TGCGCGCAGT TCTGGAGGAA 
CCCATCGATG AACGCGACAA CAAAGCCACC ATAAAGGCCA AAACCTTCTT CAAGAGCTGC 
ATGGATATTC CTCAAATCCG CAAAATCGGA ACGGGTCGAC TCAAGCAGGT ACTGCAATCC 
CTGGGCGGCT GGCCGGTCAT CGAGCGGAAC TGGAGTCCGC CGGCGGATCT CTCCGTGGAG 
CGGCTAATGG GTCAGCTCCG ACTGAACTAC AGCGAGCCGG TGATGATCGA GCTGTACGTG 
GGCGCCGACG ACAAGAACAG CTCGGTGAAC ATCCTGCAGA TGGACCAGCT GCAGTATGCC 
CTGCCCTCGA GGGACTACTA CCTGAAGGAG AGCAGTGCGA ACGACCGAAG GGCCTACCAT 
CGGTATATGA CACAGGTGGC GCTGCTCCTG GGAGCGGATC CGGCCACCGC CGCCGCGGAA 
CTGGAGAAAG TTGTGCTCTT CGAGACGCAG CTAGTGAATG TTTCCTTGCC GGAGGCGGAT 
CGTCATGACA CTAGCCTGGT CTATCGTAAG ATGTTGCTGC CCGAGCTGCA GGAACTGGTG 
CCCGAGGTGC AGTGGCAGGA GTATCTACAG GCAGCTCTGG GTCCGGGGAT TCCCCTGCAG 
GAGGACGAGC CGCTGGTCAC CTACGGGCTG CACTATCTCA CCGAAATGGG CAAGATACTG 
GCGCACACCG ATCGCCGTGT GGTGCACAAC TATATGCTGT GGCGCCTGGT CATGTCCTTG 
ATGTCACACA TGATCGATGA GTATCAGCGT GAGCGGGTGG AGTTCCGCAA GATCCTAATG 
GGCATCCAGT CGGAGAGGAC ACGCTGGTCA CAGTGCGTCG AGTGGACTAA CAAGAAGCTG 
GGTGTCGCCG TGGGAGCCCT CTTCATCCGG GACAACTTCA ATCAGGAGAG CAAGGAGGTG 
GCCCTCGAGA TGATCCACAC TATACGTGCG GCGTTCAACG AGCTTCTGGC CGAGAACGAT 
TGGATGGACG ACGAGACGCG GGCGGTGGCC AAGGAGAAGG CGGACTCGAT GAACGAGCGG 
ATTGGTTATC CGGAGCTGCT CACCAATGCC ACCGAGCTGG AGCAGGAGTA CGTGAATCTA 
ACAATTGTCC CCGACAACTT CATCAACAAT GTCCTTAGCA TTCTGCAATG GGAGTCGGAG 
AAGATGCTGC GACTTCTTCG CCAGCCGGTG GACAAGGAAA AGTGGACCAC CGAACCGGCG 
GTGGTGAATG CCTTCTACAA CCCGAATAAA AACGATATAG TATTTCCTGC TGGCATCCTG 
CAGCCGCTAT TCTACAGCCA GCACTTTCCC AAGTCCTTGA ATTACGGCGG AATCGGAGTG 
GTCATTGGCC ACGAGATTAC CCACGGATTC GACGACAAGG GCAGGCAGTT CGACAAGGAG 
GGCAACATGA TGCAGTGGTG GAACAATGCC ACCATCGAGG CCTTTCGCGA ACGGACGCAG 
TGCGTCATCG ATCAGTACTC GCGCTACAAG ATTAACGAGG TGGACATGTT CATGGACGGA 
CGGATGACGC AGGGCGAGAA CATCGCCGAC AACGGCGGTC TAAAACAGGC TTTCAGGGCC 
TACAAGAAAT GGGAGACCTT ACATGGGCGG GAGCAGCAGC TGCCCGGCCT GAACATGACC 
CACGATCAGC TGTTCTTTCT CAACTACGCC CAAATCTGGT GCGGATCCAT GCGGCCGGAG 
GACGCTCTGA CCAAGATCCG CTCCGCGGTC CACTCGCCCG GATTCGTTCG TGTCCTGGGA 
CCACTTTCGA ATTCTCGCGA CTTTGCCAGC GCCTACAAAT GCCCACTGGG CAGCACCATG 
AATCCGGCGG AAAAGTGCAG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nep1 ( NM_078501.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511056.2 Drosophila melanogaster neprilysin 1, transcript variant A (Nep1), mRNA.

Price & Availability↑

CloneID	OFa00933
Clone ID Related Accession (Same CDS sequence)	NM_167059.2 , NM_001297996.1 , NM_001297997.1 , NM_078501.3
Accession Version	NM_078501.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neprilysin 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078501.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCAGC AACATGAGGC AACAGCGGCG GCCGCGGAGA AGCCGCTAAA TAACGGCTAC 
CTGCAGGCGA ATGCGCCGCT GGAGGAGCTG AGTGCCACGG TGGTGTCGCC ACTCCTGGGA 
CAGCAACAGG TGCAGCACCA GGCGCCACAT CAGATGCAGC AGCAACAGCA GCAGCAGCAG 
CAGAACAAGC TGCCCACTGT CGTTTTCCTG GCGCCCGACG GCAGCGGAGG CGTGGGAATC 
CAGCGAGGAA ATCCAGCGCA GGGCAATCCC GGCATGGTAA CGGGCACCGG CAGCCACAGC 
GACTGGCTGC TCAAGGAGTC GCAGCAGCGG CGCCGCCTGC TCGTCCTGGC CATCGCCTTT 
ACCGTTCTGG GCGCGGCCAT CGGGGCACTG GCCATCTACT TCGCCAGCGT CCATCAGCGT 
TGCCATCTGT ATCGCCTGGA GCCAGACAAC GATGACAGAC CGAACGGCAG GTGGAACCAG 
GATTCGGGAT CCGCCCACGA GGGGCAGGAT AACATTTGCA TGACCCAGGA GTGCGTGAGA 
ACAGCCGCCT CCCTGCTCTC GGCCATGGAC CTCAACTCCG ATCCCTGCGA GGACTTTTTC 
CAGTACGCGT GCGGCACGTG GAACAAGATG CATCCCATTC CCGAGGACCG CAGCTCCATC 
AGCACGTTTG AGGTCCTGTC CGATCAGCAG CAGGTTATCC TGCGCGCAGT TCTGGAGGAA 
CCCATCGATG AACGCGACAA CAAAGCCACC ATAAAGGCCA AAACCTTCTT CAAGAGCTGC 
ATGGATATTC CTCAAATCCG CAAAATCGGA ACGGGTCGAC TCAAGCAGGT ACTGCAATCC 
CTGGGCGGCT GGCCGGTCAT CGAGCGGAAC TGGAGTCCGC CGGCGGATCT CTCCGTGGAG 
CGGCTAATGG GTCAGCTCCG ACTGAACTAC AGCGAGCCGG TGATGATCGA GCTGTACGTG 
GGCGCCGACG ACAAGAACAG CTCGGTGAAC ATCCTGCAGA TGGACCAGCT GCAGTATGCC 
CTGCCCTCGA GGGACTACTA CCTGAAGGAG AGCAGTGCGA ACGACCGAAG GGCCTACCAT 
CGGTATATGA CACAGGTGGC GCTGCTCCTG GGAGCGGATC CGGCCACCGC CGCCGCGGAA 
CTGGAGAAAG TTGTGCTCTT CGAGACGCAG CTAGTGAATG TTTCCTTGCC GGAGGCGGAT 
CGTCATGACA CTAGCCTGGT CTATCGTAAG ATGTTGCTGC CCGAGCTGCA GGAACTGGTG 
CCCGAGGTGC AGTGGCAGGA GTATCTACAG GCAGCTCTGG GTCCGGGGAT TCCCCTGCAG 
GAGGACGAGC CGCTGGTCAC CTACGGGCTG CACTATCTCA CCGAAATGGG CAAGATACTG 
GCGCACACCG ATCGCCGTGT GGTGCACAAC TATATGCTGT GGCGCCTGGT CATGTCCTTG 
ATGTCACACA TGATCGATGA GTATCAGCGT GAGCGGGTGG AGTTCCGCAA GATCCTAATG 
GGCATCCAGT CGGAGAGGAC ACGCTGGTCA CAGTGCGTCG AGTGGACTAA CAAGAAGCTG 
GGTGTCGCCG TGGGAGCCCT CTTCATCCGG GACAACTTCA ATCAGGAGAG CAAGGAGGTG 
GCCCTCGAGA TGATCCACAC TATACGTGCG GCGTTCAACG AGCTTCTGGC CGAGAACGAT 
TGGATGGACG ACGAGACGCG GGCGGTGGCC AAGGAGAAGG CGGACTCGAT GAACGAGCGG 
ATTGGTTATC CGGAGCTGCT CACCAATGCC ACCGAGCTGG AGCAGGAGTA CGTGAATCTA 
ACAATTGTCC CCGACAACTT CATCAACAAT GTCCTTAGCA TTCTGCAATG GGAGTCGGAG 
AAGATGCTGC GACTTCTTCG CCAGCCGGTG GACAAGGAAA AGTGGACCAC CGAACCGGCG 
GTGGTGAATG CCTTCTACAA CCCGAATAAA AACGATATAG TATTTCCTGC TGGCATCCTG 
CAGCCGCTAT TCTACAGCCA GCACTTTCCC AAGTCCTTGA ATTACGGCGG AATCGGAGTG 
GTCATTGGCC ACGAGATTAC CCACGGATTC GACGACAAGG GCAGGCAGTT CGACAAGGAG 
GGCAACATGA TGCAGTGGTG GAACAATGCC ACCATCGAGG CCTTTCGCGA ACGGACGCAG 
TGCGTCATCG ATCAGTACTC GCGCTACAAG ATTAACGAGG TGGACATGTT CATGGACGGA 
CGGATGACGC AGGGCGAGAA CATCGCCGAC AACGGCGGTC TAAAACAGGC TTTCAGGGCC 
TACAAGAAAT GGGAGACCTT ACATGGGCGG GAGCAGCAGC TGCCCGGCCT GAACATGACC 
CACGATCAGC TGTTCTTTCT CAACTACGCC CAAATCTGGT GCGGATCCAT GCGGCCGGAG 
GACGCTCTGA CCAAGATCCG CTCCGCGGTC CACTCGCCCG GATTCGTTCG TGTCCTGGGA 
CCACTTTCGA ATTCTCGCGA CTTTGCCAGC GCCTACAAAT GCCCACTGGG CAGCACCATG 
AATCCGGCGG AAAAGTGCAG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511056.2
CDS	447..2996
Translation	MSQQHEATAAAAEKPLNNGYLQANAPLEELSATVVSPLLGQQQVQHQAPHQMQQQQQQQQQNKLPTVVFLAPDGSGGVGIQRGNPAQGNPGMVTGTGSHSDWLLKESQQRRRLLVLAIAFTVLGAAIGALAIYFASVHQRCHLYRLEPDNDDRPNGRWNQDSGSAHEGQDNICMTQECVRTAASLLSAMDLNSDPCEDFFQYACGTWNKMHPIPEDRSSISTFEVLSDQQQVILRAVLEEPIDERDNKATIKAKTFFKSCMDIPQIRKIGTGRLKQVLQSLGGWPVIERNWSPPADLSVERLMGQLRLNYSEPVMIELYVGADDKNSSVNILQMDQLQYALPSRDYYLKESSANDRRAYHRYMTQVALLLGADPATAAAELEKVVLFETQLVNVSLPEADRHDTSLVYRKMLLPELQELVPEVQWQEYLQAALGPGIPLQEDEPLVTYGLHYLTEMGKILAHTDRRVVHNYMLWRLVMSLMSHMIDEYQRERVEFRKILMGIQSERTRWSQCVEWTNKKLGVAVGALFIRDNFNQESKEVALEMIHTIRAAFNELLAENDWMDDETRAVAKEKADSMNERIGYPELLTNATELEQEYVNLTIVPDNFINNVLSILQWESEKMLRLLRQPVDKEKWTTEPAVVNAFYNPNKNDIVFPAGILQPLFYSQHFPKSLNYGGIGVVIGHEITHGFDDKGRQFDKEGNMMQWWNNATIEAFRERTQCVIDQYSRYKINEVDMFMDGRMTQGENIADNGGLKQAFRAYKKWETLHGREQQLPGLNMTHDQLFFLNYAQIWCGSMRPEDALTKIRSAVHSPGFVRVLGPLSNSRDFASAYKCPLGSTMNPAEKCSVW

Target ORF information:

RefSeq Version	NM_078501.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neprilysin 1, transcript variant A (Nep1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078501.3

ORF Insert Sequence:

ATGTCGCAGC AACATGAGGC AACAGCGGCG GCCGCGGAGA AGCCGCTAAA TAACGGCTAC 
CTGCAGGCGA ATGCGCCGCT GGAGGAGCTG AGTGCCACGG TGGTGTCGCC ACTCCTGGGA 
CAGCAACAGG TGCAGCACCA GGCGCCACAT CAGATGCAGC AGCAACAGCA GCAGCAGCAG 
CAGAACAAGC TGCCCACTGT CGTTTTCCTG GCGCCCGACG GCAGCGGAGG CGTGGGAATC 
CAGCGAGGAA ATCCAGCGCA GGGCAATCCC GGCATGGTAA CGGGCACCGG CAGCCACAGC 
GACTGGCTGC TCAAGGAGTC GCAGCAGCGG CGCCGCCTGC TCGTCCTGGC CATCGCCTTT 
ACCGTTCTGG GCGCGGCCAT CGGGGCACTG GCCATCTACT TCGCCAGCGT CCATCAGCGT 
TGCCATCTGT ATCGCCTGGA GCCAGACAAC GATGACAGAC CGAACGGCAG GTGGAACCAG 
GATTCGGGAT CCGCCCACGA GGGGCAGGAT AACATTTGCA TGACCCAGGA GTGCGTGAGA 
ACAGCCGCCT CCCTGCTCTC GGCCATGGAC CTCAACTCCG ATCCCTGCGA GGACTTTTTC 
CAGTACGCGT GCGGCACGTG GAACAAGATG CATCCCATTC CCGAGGACCG CAGCTCCATC 
AGCACGTTTG AGGTCCTGTC CGATCAGCAG CAGGTTATCC TGCGCGCAGT TCTGGAGGAA 
CCCATCGATG AACGCGACAA CAAAGCCACC ATAAAGGCCA AAACCTTCTT CAAGAGCTGC 
ATGGATATTC CTCAAATCCG CAAAATCGGA ACGGGTCGAC TCAAGCAGGT ACTGCAATCC 
CTGGGCGGCT GGCCGGTCAT CGAGCGGAAC TGGAGTCCGC CGGCGGATCT CTCCGTGGAG 
CGGCTAATGG GTCAGCTCCG ACTGAACTAC AGCGAGCCGG TGATGATCGA GCTGTACGTG 
GGCGCCGACG ACAAGAACAG CTCGGTGAAC ATCCTGCAGA TGGACCAGCT GCAGTATGCC 
CTGCCCTCGA GGGACTACTA CCTGAAGGAG AGCAGTGCGA ACGACCGAAG GGCCTACCAT 
CGGTATATGA CACAGGTGGC GCTGCTCCTG GGAGCGGATC CGGCCACCGC CGCCGCGGAA 
CTGGAGAAAG TTGTGCTCTT CGAGACGCAG CTAGTGAATG TTTCCTTGCC GGAGGCGGAT 
CGTCATGACA CTAGCCTGGT CTATCGTAAG ATGTTGCTGC CCGAGCTGCA GGAACTGGTG 
CCCGAGGTGC AGTGGCAGGA GTATCTACAG GCAGCTCTGG GTCCGGGGAT TCCCCTGCAG 
GAGGACGAGC CGCTGGTCAC CTACGGGCTG CACTATCTCA CCGAAATGGG CAAGATACTG 
GCGCACACCG ATCGCCGTGT GGTGCACAAC TATATGCTGT GGCGCCTGGT CATGTCCTTG 
ATGTCACACA TGATCGATGA GTATCAGCGT GAGCGGGTGG AGTTCCGCAA GATCCTAATG 
GGCATCCAGT CGGAGAGGAC ACGCTGGTCA CAGTGCGTCG AGTGGACTAA CAAGAAGCTG 
GGTGTCGCCG TGGGAGCCCT CTTCATCCGG GACAACTTCA ATCAGGAGAG CAAGGAGGTG 
GCCCTCGAGA TGATCCACAC TATACGTGCG GCGTTCAACG AGCTTCTGGC CGAGAACGAT 
TGGATGGACG ACGAGACGCG GGCGGTGGCC AAGGAGAAGG CGGACTCGAT GAACGAGCGG 
ATTGGTTATC CGGAGCTGCT CACCAATGCC ACCGAGCTGG AGCAGGAGTA CGTGAATCTA 
ACAATTGTCC CCGACAACTT CATCAACAAT GTCCTTAGCA TTCTGCAATG GGAGTCGGAG 
AAGATGCTGC GACTTCTTCG CCAGCCGGTG GACAAGGAAA AGTGGACCAC CGAACCGGCG 
GTGGTGAATG CCTTCTACAA CCCGAATAAA AACGATATAG TATTTCCTGC TGGCATCCTG 
CAGCCGCTAT TCTACAGCCA GCACTTTCCC AAGTCCTTGA ATTACGGCGG AATCGGAGTG 
GTCATTGGCC ACGAGATTAC CCACGGATTC GACGACAAGG GCAGGCAGTT CGACAAGGAG 
GGCAACATGA TGCAGTGGTG GAACAATGCC ACCATCGAGG CCTTTCGCGA ACGGACGCAG 
TGCGTCATCG ATCAGTACTC GCGCTACAAG ATTAACGAGG TGGACATGTT CATGGACGGA 
CGGATGACGC AGGGCGAGAA CATCGCCGAC AACGGCGGTC TAAAACAGGC TTTCAGGGCC 
TACAAGAAAT GGGAGACCTT ACATGGGCGG GAGCAGCAGC TGCCCGGCCT GAACATGACC 
CACGATCAGC TGTTCTTTCT CAACTACGCC CAAATCTGGT GCGGATCCAT GCGGCCGGAG 
GACGCTCTGA CCAAGATCCG CTCCGCGGTC CACTCGCCCG GATTCGTTCG TGTCCTGGGA 
CCACTTTCGA ATTCTCGCGA CTTTGCCAGC GCCTACAAAT GCCCACTGGG CAGCACCATG 
AATCCGGCGG AAAAGTGCAG CGTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nep1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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