Vsx1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vsx1
Entrez Gene ID	31470
Full Name	Visual system homeobox 1
Synonyms	CG4136,Chx1,DChx1,DVSX1,Dchx1,Dmel\CG4136,Vsx-1,dChx1,dVsx1,lincRNA.S8688
General protein information	Preferred Names Visual system homeobox 1 Names CG4136 gene product from transcript CG4136-RB CG4136-PA CG4136-PB Chx1 Vsx1-PA Vsx1-PB visual system homeobox 1 visual system homeobox 1 ortholog
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5A3-5A6

mRNA and Protein(s)

mRNA	Protein	Name
NM_132004.3	NP_572232.2	visual system homeobox 1, isoform A
NM_001297969.1	NP_001284898.1	visual system homeobox 1, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dVsx1 (CG4136) and dVsx2 (CG33980) are coexpressed in a subset of neurons in the medulla, including the transmedullary neurons that transmit visual information from photoreceptors to deeper layers of the visual system.
Title: Conserved role of the Vsx genes supports a monophyletic origin for bilaterian visual systems.

The following Vsx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vsx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00867	NM_001297969.1 Latest version!	Drosophila melanogaster visual system homeobox 1, transcript variant B (Vsx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa00867	NM_132004.3 Latest version!	Drosophila melanogaster visual system homeobox 1, transcript variant A (Vsx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vsx1 ( NM_132004.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572232.2 Drosophila melanogaster visual system homeobox 1, transcript variant A (Vsx1), mRNA.

Price & Availability↑

CloneID	OFa00867
Clone ID Related Accession (Same CDS sequence)	NM_132004.3 , NM_001297969.1
Accession Version	NM_132004.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	visual system homeobox 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132004.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGCCA CCAAGATGCG TACGCCGTTT GCCATTCAGG AGATCCTGGG ACTGGGCGCC 
TATCAGCAGC AGGCCACTGC CAGTGCCACG CCCTCCGAGG CGCCACCCTC GCCGGCCGCC 
ACGCCGCCGC CCACAGCGAA TACGCCCGCT AATGGCTCGC TATCCGCCGT ACAGCAGCAA 
CAGCAGCAGC AACAGCAGCA GCAGCAACAA CAGCAACAGC TGCAGCAGCA ACAACAGCAG 
GCGATTGATA GCCTCCGCGA ATCCCGATCC ATATCGCCGG ATGTCTCCAG ACTCTCGGCC 
GCTGCAGCGG CGGCGGCAGC GGCTGCCGCC CACTGCCAAT TGCCCGTCTT CAATCCGGCC 
AACTTCTATG CCGCCGCCGG CTATGCCGCT GCAGCGGATC CGGGCAACGC GGCTGCAGCC 
CATCACCACC ACATGACCAC CTTGGCGGCG GCCGCCTATC TCCGGGGATT CCTGCCGCCC 
GGATTCAGCA CGCCCCACGA GTTTCGGGGC CACTTCCAGC AGCACTTCGG CGCTCAGTTT 
GCAGAACAAG CTGCCGCTCG TGGCCAGGAG TACGCCAGCA GTTTGGCCAA TGCCAGTGGA 
GATGAGCAGT CGCCCAGCAA GAATAGCAGT TCATCGGGCA ACGGCGGATC CGGCGGATCG 
GGATCAGGAT CTGGTGGCGG CAATCCGACC TCGAATGGCC TCGGGCACCT GGGCTCGCCA 
ACGGCCAGCA ATGGTGGATC CGGCGGTGGA GCCGGTGTGG GCGGATCGGG CGGTGGATCG 
GGCACGTCGG GCAGCAACTC CTCGACATCC GGAAACGGCG GTAACGGCGG CAGCTCCGGC 
AGTCGCTCCT CGCCCGCTGG TCCGCATCAC TCGGCCGCAC TGGCTGCCCA TCAGCATGCA 
GCGGCTGCTG CGGCCGCCGC TCATCATCAT GCCGCTGCCG CTGCTGCCGC CCATCATGCT 
CACCACGCCC ACGCCCAGGC GCACGCTCAT GCCCACGCGC ATGTCCACGC CCATGCCGCT 
CACGCCGCCC ATGCCGCCCA CGCCCATGCC CATCATGCCG AGGCATTTCT GGGAGCAGCG 
GGCGGAGCTG GAGGCAATCC CAATAGCCTG CTGGCCGCCC ATGGTGGCGA TGTTCTTGTC 
GGTCCTGGCG GCACGGGTCC CGGCGGCAAG AAGAAGAACA AGCGACGCCA CGGACGCACG 
ATCTTCACCA GCAGCCAGCT GGAGGAGCTG GAGAAGGCCT TCAAGGAGGC ACACTACCCG 
GACGTGAGTG CGCGCGAGCT GCTGTCCATG AAAACTGGCC TCGCCGAGGA TCGCATTCAG 
GTGTGGTACC AGAATCGCCG GGCCAAGTGG CGCAAAACGG AGAAATGCTG GGGCCACAGC 
ACCAAAATGG CCGAATACGG ATTGTATGGA GCGATGGTGA GGCATTCGCT GCCACTGCCG 
GAGACCATCA TCAAGTCGGC CAAGGAGGAC GAGTCGGTGG CGCCGTGGCT GCTGGGCATG 
CACAAGAAGT CACTGGAGGC CGCCGAGCTG CTCAAGAGCG ACGAGAGTGA CCGGGAGACG 
CCCACCTCGG ACGATACGAA CACCAGCTAC TCGGCGGGCA GCACCCATAA TTCACCGATC 
TCCAGTTTCT CGATCTCGCG TCTCCTCTTT GACGCCCCAC CGCCGGCGGC GGGTTCCGCT 
GGTGGCAAGG GACACCACCA CCATCATCAC CACCACAAGG GCCACCATCA TGCCCACGTT 
CATGCCCAGG CGCAGGCGCA CGCCCACATG CTGCTGCACC ACCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAACAGCA TCACCATCAC TTGCACGCCG CAGAGAATTC CTTGCAGCAG 
CAGCAAGTTG CTGGAACGGG CTCGGGTTCG GGATCCGGTT CCGGGTCGGG AACGGGTGCG 
GGCTCGACTG GGCCACCAGC ACACCATCCG TACAACCAGC AGCAGCACCT GCACCACCTG 
CAGCAACTGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGG CGCAGCAGCA GCAGCAGCAT 
CACCACCACC AGCACCACCA TTCGGTGCAC CATATGCACC ACCACAATGC TGCGGCCGGC 
TACGCCGAGG CAGCGGTGGC TGCTGCAGCG GCCGCAGCAG CTGTTGCCGC AGCAGCAAAC 
GCACGGAATG CAGCGGCGGC AGCAGCGGTG GCCGCAGCCT CAGCGTCCGC CTCCGCAGCC 
GTGACCATTG CGGAATCGGA GGCGGAGGAG GAGCAGGACG ACATCGAGGA CATGGAACTG 
GACAAGGACT GTGAGGTGGA GGAGGATGTG GAGCCCGTGG ATAACGATAC CAATACCAAC 
AACGAGCACG ACAACGAGTG CGATGCGGAT GCGGATGTTG AGGCGGATGT GGATGCGGAT 
GCGGATGCCG ATCCCGAGAT AGACATCTGC GACGACCACG ACGAGGAGAT GCGTCTGCAG 
GAGCAGCTCC TGCTGAAAGT GAAGCAGGAG CAGGCGCATC TGGGGTCCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572232.2
CDS	293..2806
Translation	MQATKMRTPFAIQEILGLGAYQQQATASATPSEAPPSPAATPPPTANTPANGSLSAVQQQQQQQQQQQQQQQQLQQQQQQAIDSLRESRSISPDVSRLSAAAAAAAAAAAHCQLPVFNPANFYAAAGYAAAADPGNAAAAHHHHMTTLAAAAYLRGFLPPGFSTPHEFRGHFQQHFGAQFAEQAAARGQEYASSLANASGDEQSPSKNSSSSGNGGSGGSGSGSGGGNPTSNGLGHLGSPTASNGGSGGGAGVGGSGGGSGTSGSNSSTSGNGGNGGSSGSRSSPAGPHHSAALAAHQHAAAAAAAAHHHAAAAAAAHHAHHAHAQAHAHAHAHVHAHAAHAAHAAHAHAHHAEAFLGAAGGAGGNPNSLLAAHGGDVLVGPGGTGPGGKKKNKRRHGRTIFTSSQLEELEKAFKEAHYPDVSARELLSMKTGLAEDRIQVWYQNRRAKWRKTEKCWGHSTKMAEYGLYGAMVRHSLPLPETIIKSAKEDESVAPWLLGMHKKSLEAAELLKSDESDRETPTSDDTNTSYSAGSTHNSPISSFSISRLLFDAPPPAAGSAGGKGHHHHHHHHKGHHHAHVHAQAQAHAHMLLHHQQQQQQQQQQQQHHHHLHAAENSLQQQQVAGTGSGSGSGSGSGTGAGSTGPPAHHPYNQQQHLHHLQQLQQQQQQQQQQAQQQQQHHHHQHHHSVHHMHHHNAAAGYAEAAVAAAAAAAAVAAAANARNAAAAAAVAAASASASAAVTIAESEAEEEQDDIEDMELDKDCEVEEDVEPVDNDTNTNNEHDNECDADADVEADVDADADADPEIDICDDHDEEMRLQEQLLLKVKQEQAHLGSR

Target ORF information:

RefSeq Version	NM_132004.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster visual system homeobox 1, transcript variant A (Vsx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132004.3

ORF Insert Sequence:

ATGCAGGCCA CCAAGATGCG TACGCCGTTT GCCATTCAGG AGATCCTGGG ACTGGGCGCC 
TATCAGCAGC AGGCCACTGC CAGTGCCACG CCCTCCGAGG CGCCACCCTC GCCGGCCGCC 
ACGCCGCCGC CCACAGCGAA TACGCCCGCT AATGGCTCGC TATCCGCCGT ACAGCAGCAA 
CAGCAGCAGC AACAGCAGCA GCAGCAACAA CAGCAACAGC TGCAGCAGCA ACAACAGCAG 
GCGATTGATA GCCTCCGCGA ATCCCGATCC ATATCGCCGG ATGTCTCCAG ACTCTCGGCC 
GCTGCAGCGG CGGCGGCAGC GGCTGCCGCC CACTGCCAAT TGCCCGTCTT CAATCCGGCC 
AACTTCTATG CCGCCGCCGG CTATGCCGCT GCAGCGGATC CGGGCAACGC GGCTGCAGCC 
CATCACCACC ACATGACCAC CTTGGCGGCG GCCGCCTATC TCCGGGGATT CCTGCCGCCC 
GGATTCAGCA CGCCCCACGA GTTTCGGGGC CACTTCCAGC AGCACTTCGG CGCTCAGTTT 
GCAGAACAAG CTGCCGCTCG TGGCCAGGAG TACGCCAGCA GTTTGGCCAA TGCCAGTGGA 
GATGAGCAGT CGCCCAGCAA GAATAGCAGT TCATCGGGCA ACGGCGGATC CGGCGGATCG 
GGATCAGGAT CTGGTGGCGG CAATCCGACC TCGAATGGCC TCGGGCACCT GGGCTCGCCA 
ACGGCCAGCA ATGGTGGATC CGGCGGTGGA GCCGGTGTGG GCGGATCGGG CGGTGGATCG 
GGCACGTCGG GCAGCAACTC CTCGACATCC GGAAACGGCG GTAACGGCGG CAGCTCCGGC 
AGTCGCTCCT CGCCCGCTGG TCCGCATCAC TCGGCCGCAC TGGCTGCCCA TCAGCATGCA 
GCGGCTGCTG CGGCCGCCGC TCATCATCAT GCCGCTGCCG CTGCTGCCGC CCATCATGCT 
CACCACGCCC ACGCCCAGGC GCACGCTCAT GCCCACGCGC ATGTCCACGC CCATGCCGCT 
CACGCCGCCC ATGCCGCCCA CGCCCATGCC CATCATGCCG AGGCATTTCT GGGAGCAGCG 
GGCGGAGCTG GAGGCAATCC CAATAGCCTG CTGGCCGCCC ATGGTGGCGA TGTTCTTGTC 
GGTCCTGGCG GCACGGGTCC CGGCGGCAAG AAGAAGAACA AGCGACGCCA CGGACGCACG 
ATCTTCACCA GCAGCCAGCT GGAGGAGCTG GAGAAGGCCT TCAAGGAGGC ACACTACCCG 
GACGTGAGTG CGCGCGAGCT GCTGTCCATG AAAACTGGCC TCGCCGAGGA TCGCATTCAG 
GTGTGGTACC AGAATCGCCG GGCCAAGTGG CGCAAAACGG AGAAATGCTG GGGCCACAGC 
ACCAAAATGG CCGAATACGG ATTGTATGGA GCGATGGTGA GGCATTCGCT GCCACTGCCG 
GAGACCATCA TCAAGTCGGC CAAGGAGGAC GAGTCGGTGG CGCCGTGGCT GCTGGGCATG 
CACAAGAAGT CACTGGAGGC CGCCGAGCTG CTCAAGAGCG ACGAGAGTGA CCGGGAGACG 
CCCACCTCGG ACGATACGAA CACCAGCTAC TCGGCGGGCA GCACCCATAA TTCACCGATC 
TCCAGTTTCT CGATCTCGCG TCTCCTCTTT GACGCCCCAC CGCCGGCGGC GGGTTCCGCT 
GGTGGCAAGG GACACCACCA CCATCATCAC CACCACAAGG GCCACCATCA TGCCCACGTT 
CATGCCCAGG CGCAGGCGCA CGCCCACATG CTGCTGCACC ACCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAACAGCA TCACCATCAC TTGCACGCCG CAGAGAATTC CTTGCAGCAG 
CAGCAAGTTG CTGGAACGGG CTCGGGTTCG GGATCCGGTT CCGGGTCGGG AACGGGTGCG 
GGCTCGACTG GGCCACCAGC ACACCATCCG TACAACCAGC AGCAGCACCT GCACCACCTG 
CAGCAACTGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGG CGCAGCAGCA GCAGCAGCAT 
CACCACCACC AGCACCACCA TTCGGTGCAC CATATGCACC ACCACAATGC TGCGGCCGGC 
TACGCCGAGG CAGCGGTGGC TGCTGCAGCG GCCGCAGCAG CTGTTGCCGC AGCAGCAAAC 
GCACGGAATG CAGCGGCGGC AGCAGCGGTG GCCGCAGCCT CAGCGTCCGC CTCCGCAGCC 
GTGACCATTG CGGAATCGGA GGCGGAGGAG GAGCAGGACG ACATCGAGGA CATGGAACTG 
GACAAGGACT GTGAGGTGGA GGAGGATGTG GAGCCCGTGG ATAACGATAC CAATACCAAC 
AACGAGCACG ACAACGAGTG CGATGCGGAT GCGGATGTTG AGGCGGATGT GGATGCGGAT 
GCGGATGCCG ATCCCGAGAT AGACATCTGC GACGACCACG ACGAGGAGAT GCGTCTGCAG 
GAGCAGCTCC TGCTGAAAGT GAAGCAGGAG CAGGCGCATC TGGGGTCCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vsx1 ( NM_001297969.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284898.1 Drosophila melanogaster visual system homeobox 1, transcript variant B (Vsx1), mRNA.

Price & Availability↑

CloneID	OFa00867
Clone ID Related Accession (Same CDS sequence)	NM_132004.3 , NM_001297969.1
Accession Version	NM_001297969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	visual system homeobox 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGCCA CCAAGATGCG TACGCCGTTT GCCATTCAGG AGATCCTGGG ACTGGGCGCC 
TATCAGCAGC AGGCCACTGC CAGTGCCACG CCCTCCGAGG CGCCACCCTC GCCGGCCGCC 
ACGCCGCCGC CCACAGCGAA TACGCCCGCT AATGGCTCGC TATCCGCCGT ACAGCAGCAA 
CAGCAGCAGC AACAGCAGCA GCAGCAACAA CAGCAACAGC TGCAGCAGCA ACAACAGCAG 
GCGATTGATA GCCTCCGCGA ATCCCGATCC ATATCGCCGG ATGTCTCCAG ACTCTCGGCC 
GCTGCAGCGG CGGCGGCAGC GGCTGCCGCC CACTGCCAAT TGCCCGTCTT CAATCCGGCC 
AACTTCTATG CCGCCGCCGG CTATGCCGCT GCAGCGGATC CGGGCAACGC GGCTGCAGCC 
CATCACCACC ACATGACCAC CTTGGCGGCG GCCGCCTATC TCCGGGGATT CCTGCCGCCC 
GGATTCAGCA CGCCCCACGA GTTTCGGGGC CACTTCCAGC AGCACTTCGG CGCTCAGTTT 
GCAGAACAAG CTGCCGCTCG TGGCCAGGAG TACGCCAGCA GTTTGGCCAA TGCCAGTGGA 
GATGAGCAGT CGCCCAGCAA GAATAGCAGT TCATCGGGCA ACGGCGGATC CGGCGGATCG 
GGATCAGGAT CTGGTGGCGG CAATCCGACC TCGAATGGCC TCGGGCACCT GGGCTCGCCA 
ACGGCCAGCA ATGGTGGATC CGGCGGTGGA GCCGGTGTGG GCGGATCGGG CGGTGGATCG 
GGCACGTCGG GCAGCAACTC CTCGACATCC GGAAACGGCG GTAACGGCGG CAGCTCCGGC 
AGTCGCTCCT CGCCCGCTGG TCCGCATCAC TCGGCCGCAC TGGCTGCCCA TCAGCATGCA 
GCGGCTGCTG CGGCCGCCGC TCATCATCAT GCCGCTGCCG CTGCTGCCGC CCATCATGCT 
CACCACGCCC ACGCCCAGGC GCACGCTCAT GCCCACGCGC ATGTCCACGC CCATGCCGCT 
CACGCCGCCC ATGCCGCCCA CGCCCATGCC CATCATGCCG AGGCATTTCT GGGAGCAGCG 
GGCGGAGCTG GAGGCAATCC CAATAGCCTG CTGGCCGCCC ATGGTGGCGA TGTTCTTGTC 
GGTCCTGGCG GCACGGGTCC CGGCGGCAAG AAGAAGAACA AGCGACGCCA CGGACGCACG 
ATCTTCACCA GCAGCCAGCT GGAGGAGCTG GAGAAGGCCT TCAAGGAGGC ACACTACCCG 
GACGTGAGTG CGCGCGAGCT GCTGTCCATG AAAACTGGCC TCGCCGAGGA TCGCATTCAG 
GTGTGGTACC AGAATCGCCG GGCCAAGTGG CGCAAAACGG AGAAATGCTG GGGCCACAGC 
ACCAAAATGG CCGAATACGG ATTGTATGGA GCGATGGTGA GGCATTCGCT GCCACTGCCG 
GAGACCATCA TCAAGTCGGC CAAGGAGGAC GAGTCGGTGG CGCCGTGGCT GCTGGGCATG 
CACAAGAAGT CACTGGAGGC CGCCGAGCTG CTCAAGAGCG ACGAGAGTGA CCGGGAGACG 
CCCACCTCGG ACGATACGAA CACCAGCTAC TCGGCGGGCA GCACCCATAA TTCACCGATC 
TCCAGTTTCT CGATCTCGCG TCTCCTCTTT GACGCCCCAC CGCCGGCGGC GGGTTCCGCT 
GGTGGCAAGG GACACCACCA CCATCATCAC CACCACAAGG GCCACCATCA TGCCCACGTT 
CATGCCCAGG CGCAGGCGCA CGCCCACATG CTGCTGCACC ACCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAACAGCA TCACCATCAC TTGCACGCCG CAGAGAATTC CTTGCAGCAG 
CAGCAAGTTG CTGGAACGGG CTCGGGTTCG GGATCCGGTT CCGGGTCGGG AACGGGTGCG 
GGCTCGACTG GGCCACCAGC ACACCATCCG TACAACCAGC AGCAGCACCT GCACCACCTG 
CAGCAACTGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGG CGCAGCAGCA GCAGCAGCAT 
CACCACCACC AGCACCACCA TTCGGTGCAC CATATGCACC ACCACAATGC TGCGGCCGGC 
TACGCCGAGG CAGCGGTGGC TGCTGCAGCG GCCGCAGCAG CTGTTGCCGC AGCAGCAAAC 
GCACGGAATG CAGCGGCGGC AGCAGCGGTG GCCGCAGCCT CAGCGTCCGC CTCCGCAGCC 
GTGACCATTG CGGAATCGGA GGCGGAGGAG GAGCAGGACG ACATCGAGGA CATGGAACTG 
GACAAGGACT GTGAGGTGGA GGAGGATGTG GAGCCCGTGG ATAACGATAC CAATACCAAC 
AACGAGCACG ACAACGAGTG CGATGCGGAT GCGGATGTTG AGGCGGATGT GGATGCGGAT 
GCGGATGCCG ATCCCGAGAT AGACATCTGC GACGACCACG ACGAGGAGAT GCGTCTGCAG 
GAGCAGCTCC TGCTGAAAGT GAAGCAGGAG CAGGCGCATC TGGGGTCCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284898.1
CDS	293..2806
Translation	MQATKMRTPFAIQEILGLGAYQQQATASATPSEAPPSPAATPPPTANTPANGSLSAVQQQQQQQQQQQQQQQQLQQQQQQAIDSLRESRSISPDVSRLSAAAAAAAAAAAHCQLPVFNPANFYAAAGYAAAADPGNAAAAHHHHMTTLAAAAYLRGFLPPGFSTPHEFRGHFQQHFGAQFAEQAAARGQEYASSLANASGDEQSPSKNSSSSGNGGSGGSGSGSGGGNPTSNGLGHLGSPTASNGGSGGGAGVGGSGGGSGTSGSNSSTSGNGGNGGSSGSRSSPAGPHHSAALAAHQHAAAAAAAAHHHAAAAAAAHHAHHAHAQAHAHAHAHVHAHAAHAAHAAHAHAHHAEAFLGAAGGAGGNPNSLLAAHGGDVLVGPGGTGPGGKKKNKRRHGRTIFTSSQLEELEKAFKEAHYPDVSARELLSMKTGLAEDRIQVWYQNRRAKWRKTEKCWGHSTKMAEYGLYGAMVRHSLPLPETIIKSAKEDESVAPWLLGMHKKSLEAAELLKSDESDRETPTSDDTNTSYSAGSTHNSPISSFSISRLLFDAPPPAAGSAGGKGHHHHHHHHKGHHHAHVHAQAQAHAHMLLHHQQQQQQQQQQQQHHHHLHAAENSLQQQQVAGTGSGSGSGSGSGTGAGSTGPPAHHPYNQQQHLHHLQQLQQQQQQQQQQAQQQQQHHHHQHHHSVHHMHHHNAAAGYAEAAVAAAAAAAAVAAAANARNAAAAAAVAAASASASAAVTIAESEAEEEQDDIEDMELDKDCEVEEDVEPVDNDTNTNNEHDNECDADADVEADVDADADADPEIDICDDHDEEMRLQEQLLLKVKQEQAHLGSR

Target ORF information:

RefSeq Version	NM_001297969.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster visual system homeobox 1, transcript variant B (Vsx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297969.1

ORF Insert Sequence:

ATGCAGGCCA CCAAGATGCG TACGCCGTTT GCCATTCAGG AGATCCTGGG ACTGGGCGCC 
TATCAGCAGC AGGCCACTGC CAGTGCCACG CCCTCCGAGG CGCCACCCTC GCCGGCCGCC 
ACGCCGCCGC CCACAGCGAA TACGCCCGCT AATGGCTCGC TATCCGCCGT ACAGCAGCAA 
CAGCAGCAGC AACAGCAGCA GCAGCAACAA CAGCAACAGC TGCAGCAGCA ACAACAGCAG 
GCGATTGATA GCCTCCGCGA ATCCCGATCC ATATCGCCGG ATGTCTCCAG ACTCTCGGCC 
GCTGCAGCGG CGGCGGCAGC GGCTGCCGCC CACTGCCAAT TGCCCGTCTT CAATCCGGCC 
AACTTCTATG CCGCCGCCGG CTATGCCGCT GCAGCGGATC CGGGCAACGC GGCTGCAGCC 
CATCACCACC ACATGACCAC CTTGGCGGCG GCCGCCTATC TCCGGGGATT CCTGCCGCCC 
GGATTCAGCA CGCCCCACGA GTTTCGGGGC CACTTCCAGC AGCACTTCGG CGCTCAGTTT 
GCAGAACAAG CTGCCGCTCG TGGCCAGGAG TACGCCAGCA GTTTGGCCAA TGCCAGTGGA 
GATGAGCAGT CGCCCAGCAA GAATAGCAGT TCATCGGGCA ACGGCGGATC CGGCGGATCG 
GGATCAGGAT CTGGTGGCGG CAATCCGACC TCGAATGGCC TCGGGCACCT GGGCTCGCCA 
ACGGCCAGCA ATGGTGGATC CGGCGGTGGA GCCGGTGTGG GCGGATCGGG CGGTGGATCG 
GGCACGTCGG GCAGCAACTC CTCGACATCC GGAAACGGCG GTAACGGCGG CAGCTCCGGC 
AGTCGCTCCT CGCCCGCTGG TCCGCATCAC TCGGCCGCAC TGGCTGCCCA TCAGCATGCA 
GCGGCTGCTG CGGCCGCCGC TCATCATCAT GCCGCTGCCG CTGCTGCCGC CCATCATGCT 
CACCACGCCC ACGCCCAGGC GCACGCTCAT GCCCACGCGC ATGTCCACGC CCATGCCGCT 
CACGCCGCCC ATGCCGCCCA CGCCCATGCC CATCATGCCG AGGCATTTCT GGGAGCAGCG 
GGCGGAGCTG GAGGCAATCC CAATAGCCTG CTGGCCGCCC ATGGTGGCGA TGTTCTTGTC 
GGTCCTGGCG GCACGGGTCC CGGCGGCAAG AAGAAGAACA AGCGACGCCA CGGACGCACG 
ATCTTCACCA GCAGCCAGCT GGAGGAGCTG GAGAAGGCCT TCAAGGAGGC ACACTACCCG 
GACGTGAGTG CGCGCGAGCT GCTGTCCATG AAAACTGGCC TCGCCGAGGA TCGCATTCAG 
GTGTGGTACC AGAATCGCCG GGCCAAGTGG CGCAAAACGG AGAAATGCTG GGGCCACAGC 
ACCAAAATGG CCGAATACGG ATTGTATGGA GCGATGGTGA GGCATTCGCT GCCACTGCCG 
GAGACCATCA TCAAGTCGGC CAAGGAGGAC GAGTCGGTGG CGCCGTGGCT GCTGGGCATG 
CACAAGAAGT CACTGGAGGC CGCCGAGCTG CTCAAGAGCG ACGAGAGTGA CCGGGAGACG 
CCCACCTCGG ACGATACGAA CACCAGCTAC TCGGCGGGCA GCACCCATAA TTCACCGATC 
TCCAGTTTCT CGATCTCGCG TCTCCTCTTT GACGCCCCAC CGCCGGCGGC GGGTTCCGCT 
GGTGGCAAGG GACACCACCA CCATCATCAC CACCACAAGG GCCACCATCA TGCCCACGTT 
CATGCCCAGG CGCAGGCGCA CGCCCACATG CTGCTGCACC ACCAGCAGCA GCAGCAGCAA 
CAGCAGCAGC AGCAACAGCA TCACCATCAC TTGCACGCCG CAGAGAATTC CTTGCAGCAG 
CAGCAAGTTG CTGGAACGGG CTCGGGTTCG GGATCCGGTT CCGGGTCGGG AACGGGTGCG 
GGCTCGACTG GGCCACCAGC ACACCATCCG TACAACCAGC AGCAGCACCT GCACCACCTG 
CAGCAACTGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGG CGCAGCAGCA GCAGCAGCAT 
CACCACCACC AGCACCACCA TTCGGTGCAC CATATGCACC ACCACAATGC TGCGGCCGGC 
TACGCCGAGG CAGCGGTGGC TGCTGCAGCG GCCGCAGCAG CTGTTGCCGC AGCAGCAAAC 
GCACGGAATG CAGCGGCGGC AGCAGCGGTG GCCGCAGCCT CAGCGTCCGC CTCCGCAGCC 
GTGACCATTG CGGAATCGGA GGCGGAGGAG GAGCAGGACG ACATCGAGGA CATGGAACTG 
GACAAGGACT GTGAGGTGGA GGAGGATGTG GAGCCCGTGG ATAACGATAC CAATACCAAC 
AACGAGCACG ACAACGAGTG CGATGCGGAT GCGGATGTTG AGGCGGATGT GGATGCGGAT 
GCGGATGCCG ATCCCGAGAT AGACATCTGC GACGACCACG ACGAGGAGAT GCGTCTGCAG 
GAGCAGCTCC TGCTGAAAGT GAAGCAGGAG CAGGCGCATC TGGGGTCCAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vsx1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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