bi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bi
Entrez Gene ID	31379
Full Name	bifid
Synonyms	BI,CG3578,Dm-OMB,Dm-omb,Dmel\CG3578,OMB,Omb,Qd,T3,TBX2,dm-omb,l(1)bi,l(1)omb,omb
General protein information	Preferred Names bifid Names CG3578 gene product from transcript CG3578-RA CG3578-PA CG3578-PB CG3578-PC CG3578-PD CG3578-PE CG3578-PF CG3578-PG Optimotor-blind bi-PA bi-PB bi-PC bi-PD bi-PE bi-PF bi-PG lethal(l)optomotor-blind optimotor blind optomer-blind optomoter blind optomoter-blind optomotor blind optomotor-blind optomotor-blind gene optomotorblind quadroon
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 4C3-4C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_080331.3	NP_525070.2	bifid, isoform A
NM_001169192.2	NP_001162663.1	bifid, isoform C
NM_001258599.2	NP_001245528.1	bifid, isoform E
NM_001297932.1	NP_001284861.1	bifid, isoform G
NM_001258598.3	NP_001245527.1	bifid, isoform D
NM_001272309.2	NP_001259238.1	bifid, isoform F
NM_001169191.1	NP_001162662.1	bifid, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

complementary expression of omb and Iro-C establishes the wing hinge/notum fold formation
Title: Complementary expression of optomotor-blind and the Iroquois complex promotes fold formation to separate wing notum and hinge territories.

Optomotor-blind does not prevent apoptosis in the haltere but rather limits growth by repressing several Decapentaplegic and Hedgehog targets.
Title: The transcription factor optomotor-blind antagonizes Drosophila haltere growth by repressing decapentaplegic and hedgehog targets.

results suggest that the primary effect of Omb is the blocking of Jak/STAT signaling by repressing transcription of upd which encodes the Jak receptor ligand Unpaired.
Title: Optomotor-blind negatively regulates Drosophila eye development by blocking Jak/STAT signaling.

Since loss of omb disrupts horizontal system and vertical system cell development, we assume that HS/VS ontogeny is controlled by Omb target genes.
Title: Optomotor-blind in the development of the Drosophila HS and VS lobula plate tangential cells.

Dpp, via omb, promoted proliferation in the lateral and repressed proliferation in the medial wing disc. Omb controlled the regional proliferation rate by oppositely regulating transcription of the microRNA gene bantam in medial versus lateral wing disc.
Title: Dpp signaling inhibits proliferation in the Drosophila wing by Omb-dependent regional control of bantam.

The following bi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00775	NM_001169191.1 Latest version!	Drosophila melanogaster bifid (bi), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa00775	NM_001169192.2 Latest version!	Drosophila melanogaster bifid (bi), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa00775	NM_080331.3 Latest version!	Drosophila melanogaster bifid (bi), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa03755	NM_001272309.2 Latest version!	Drosophila melanogaster bifid (bi), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa03756	NM_001258599.2 Latest version!	Drosophila melanogaster bifid (bi), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa03757	NM_001258598.3 Latest version!	Drosophila melanogaster bifid (bi), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa21357	NM_001297932.1 Latest version!	Drosophila melanogaster bifid (bi), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bi ( NM_080331.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525070.2 Drosophila melanogaster bifid (bi), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00775
Clone ID Related Accession (Same CDS sequence)	NM_080331.3 , NM_001169192.2 , NM_001169191.1
Accession Version	NM_080331.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bifid, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_080331.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTGTAAGTAC TACGATCCTG AACTCGATGC ACAAGTACCA GCCGCGTTTC 
CACCTGGTGC GAGCCAATGA CATCCTGAAG CTGCCGTACT CCACGTTTCG CACGTACGTC 
TTCAAGGAGA CCGAGTTCAT CGCCGTCACA GCATATCAAA ATGAGAAGAT AACTCAATTG 
AAAATCGATA ACAATCCGTT TGCGAAGGGC TTTCGTGATA CTGGTGCCGG CAAGCGGGAA 
AAGAAGCAGG CACTGATGTC GAACCGAGGG TCCGATTCGG ACAAGTTGAA TCCGACGCAT 
GTGAGCAGCT CGCGGGCACC GCTCCACCTG GGCCACGCCG GCCGTCCGCC TCATCTGCAT 
CCCCATGCCG CCCTGCTTGA TAATCAGCAG GACGACGACG ACAAGCTCCT GGACGTGGTG 
GGTCCGCCAC AGAGTCCGCT ACTCCCGCTC AGCCACTCGC TGCAGCAGAT GCACGCCCAC 
CAGCACTCCG CAGCTCTGGC TGCCTGGTTT AATCACTTGG CTGGCGCTGG AGCCGGAGCC 
TCCGAACATG CAGCTGCGGC GGCGGCCAAT GCCAGTGCGG AGGATGCACT GCGTCGTCGC 
TTGCAGGCGG ACGCGGATGT GGAGCGGGAT GGCAGCGATT CGAGTTGCTC GGAAAGCGTC 
GGCGGCAGTA CCGGCGGTGC CTTTAGGCCC ACCTCGACGG GCAGTCCCAA GGAGGCGGTG 
GGCGCGGCTG CAGCTGCCGC AGCGGCTGGC TTGAATCCCG GTGGCGGTAG CTATCCGTCA 
CCGAATATAT CGGTGGGTCC GCCCATTCAC CCGTCGCCGC ATTTGTTGCC TTACCTGTAT 
CCACATGGCC TGTATCCACC GCCGCATCTG GGCCTGCTGC ACAATCCCGC TGCAGCGGCG 
GCCATGAGTC CGGCTGGCCT GAATCCGGGT CTGCTCTTCA ATGCCCAACT GGCGCTGGCC 
GCCCAGCATC CGGCTTTGTT TGGCCACGCC TACGCGGCGG CGGGTCACAC ACCGGTATCA 
CCATTACAAG GCCTAAAGAG CCATCGCTTT TCGCCGTACA GTTTGCCCGG CAGTTTGGGC 
TCCGCCTTCG ATGCAGTGAC TCCTGGATCG AACGCAAATC GCTCGGGAGA TCCTCCAGGC 
GGCGGCGGCG GCGGCCTGGG TGGTGGTGTG GTGGAGAATG GTCCCAGGAG TCTGAGTTCC 
AGCCCTCGTC CCCGACCTGC CTCCCACTCG CCGCCCACTC GACCGATTTC GATGTCACCC 
ACAACGCCGC CATCCCTGAT GAAGCAACCA CGTGGCGGTG GTGCTGGCGC CGGTGTGGCC 
CAATCGCAGC ACTCGCCTTC GGAACTTAAG AGCATGGAGA AGATGGTCAA CGGACTGGAG 
GTTCAACACA ATGGCAGTGC GGCGGCAGCA GCGGCGGCTC TTCAGCTGGC CGAGGAAGCT 
GCCCAGCATC ATCATCACAC CCAGGCGCAC CACCAGCAGC AGCAGCATCA GTCGCACCAC 
CAGCAACAGC ACCACCAGCA ACCGGCACAA CCACATCCGC ACCACCAGAC CCATCTACAC 
TCGCACCATG GGGCGACAAC GGGCGGTACG GATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525070.2
CDS	303..3221
Translation	MRYDVQELLFHQSAEDPFARFANGMAYHPFLQLTQRPTDFSVSSLLTAGSNNNNSGNTNSGNNNSNSNNNTNSNTNNTNNLVAVSPTGGGAQLSPQSNHSSSNTTTTSNTNNSSSNNNNNNSTHNNNNNHTNNNNNNNNNTSQKQGHHLSTTEEPPSPAGTPPPTIVGLPPIPPPNNNSSSSSSNNSASAAAHPSHHPTAAHHSPSTGAAAPPAGATGLPPPTPPHHLQQQQQQQQHPAPPPPPYFPAAALAALAGSPAGPHPGLYPGGGLRFPPHHPGAHPHAHHLGSAYTTAEDVVLASAVAHQLHPAMRPLRALQPEDDGVVDDPKVTLEGKDLWEKFHKLGTEMVITKSGRQMFPQMKFRVSGLDAKAKYILLLDIVAADDYRYKFHNSRWMVAGKADPEMPKRMYIHPDSPTTGEQWMQKVVSFHKLKLTNNISDKHGFVSTTILNSMHKYQPRFHLVRANDILKLPYSTFRTYVFKETEFIAVTAYQNEKITQLKIDNNPFAKGFRDTGAGKREKKQALMSNRGSDSDKLNPTHVSSSRAPLHLGHAGRPPHLHPHAALLDNQQDDDDKLLDVVGPPQSPLLPLSHSLQQMHAHQHSAALAAWFNHLAGAGAGASEHAAAAAANASAEDALRRRLQADADVERDGSDSSCSESVGGSTGGAFRPTSTGSPKEAVGAAAAAAAAGLNPGGGSYPSPNISVGPPIHPSPHLLPYLYPHGLYPPPHLGLLHNPAAAAAMSPAGLNPGLLFNAQLALAAQHPALFGHAYAAAGHTPVSPLQGLKSHRFSPYSLPGSLGSAFDAVTPGSNANRSGDPPGGGGGGLGGGVVENGPRSLSSSPRPRPASHSPPTRPISMSPTTPPSLMKQPRGGGAGAGVAQSQHSPSELKSMEKMVNGLEVQHNGSAAAAAAALQLAEEAAQHHHHTQAHHQQQQHQSHHQQQHHQQPAQPHPHHQTHLHSHHGATTGGTDQ

Target ORF information:

RefSeq Version	NM_080331.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bifid (bi), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080331.3

ORF Insert Sequence:

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTGTAAGTAC TACGATCCTG AACTCGATGC ACAAGTACCA GCCGCGTTTC 
CACCTGGTGC GAGCCAATGA CATCCTGAAG CTGCCGTACT CCACGTTTCG CACGTACGTC 
TTCAAGGAGA CCGAGTTCAT CGCCGTCACA GCATATCAAA ATGAGAAGAT AACTCAATTG 
AAAATCGATA ACAATCCGTT TGCGAAGGGC TTTCGTGATA CTGGTGCCGG CAAGCGGGAA 
AAGAAGCAGG CACTGATGTC GAACCGAGGG TCCGATTCGG ACAAGTTGAA TCCGACGCAT 
GTGAGCAGCT CGCGGGCACC GCTCCACCTG GGCCACGCCG GCCGTCCGCC TCATCTGCAT 
CCCCATGCCG CCCTGCTTGA TAATCAGCAG GACGACGACG ACAAGCTCCT GGACGTGGTG 
GGTCCGCCAC AGAGTCCGCT ACTCCCGCTC AGCCACTCGC TGCAGCAGAT GCACGCCCAC 
CAGCACTCCG CAGCTCTGGC TGCCTGGTTT AATCACTTGG CTGGCGCTGG AGCCGGAGCC 
TCCGAACATG CAGCTGCGGC GGCGGCCAAT GCCAGTGCGG AGGATGCACT GCGTCGTCGC 
TTGCAGGCGG ACGCGGATGT GGAGCGGGAT GGCAGCGATT CGAGTTGCTC GGAAAGCGTC 
GGCGGCAGTA CCGGCGGTGC CTTTAGGCCC ACCTCGACGG GCAGTCCCAA GGAGGCGGTG 
GGCGCGGCTG CAGCTGCCGC AGCGGCTGGC TTGAATCCCG GTGGCGGTAG CTATCCGTCA 
CCGAATATAT CGGTGGGTCC GCCCATTCAC CCGTCGCCGC ATTTGTTGCC TTACCTGTAT 
CCACATGGCC TGTATCCACC GCCGCATCTG GGCCTGCTGC ACAATCCCGC TGCAGCGGCG 
GCCATGAGTC CGGCTGGCCT GAATCCGGGT CTGCTCTTCA ATGCCCAACT GGCGCTGGCC 
GCCCAGCATC CGGCTTTGTT TGGCCACGCC TACGCGGCGG CGGGTCACAC ACCGGTATCA 
CCATTACAAG GCCTAAAGAG CCATCGCTTT TCGCCGTACA GTTTGCCCGG CAGTTTGGGC 
TCCGCCTTCG ATGCAGTGAC TCCTGGATCG AACGCAAATC GCTCGGGAGA TCCTCCAGGC 
GGCGGCGGCG GCGGCCTGGG TGGTGGTGTG GTGGAGAATG GTCCCAGGAG TCTGAGTTCC 
AGCCCTCGTC CCCGACCTGC CTCCCACTCG CCGCCCACTC GACCGATTTC GATGTCACCC 
ACAACGCCGC CATCCCTGAT GAAGCAACCA CGTGGCGGTG GTGCTGGCGC CGGTGTGGCC 
CAATCGCAGC ACTCGCCTTC GGAACTTAAG AGCATGGAGA AGATGGTCAA CGGACTGGAG 
GTTCAACACA ATGGCAGTGC GGCGGCAGCA GCGGCGGCTC TTCAGCTGGC CGAGGAAGCT 
GCCCAGCATC ATCATCACAC CCAGGCGCAC CACCAGCAGC AGCAGCATCA GTCGCACCAC 
CAGCAACAGC ACCACCAGCA ACCGGCACAA CCACATCCGC ACCACCAGAC CCATCTACAC 
TCGCACCATG GGGCGACAAC GGGCGGTACG GATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bi ( NM_001169192.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162663.1 Drosophila melanogaster bifid (bi), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00775
Clone ID Related Accession (Same CDS sequence)	NM_080331.3 , NM_001169192.2 , NM_001169191.1
Accession Version	NM_001169192.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bifid, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001169192.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTGTAAGTAC TACGATCCTG AACTCGATGC ACAAGTACCA GCCGCGTTTC 
CACCTGGTGC GAGCCAATGA CATCCTGAAG CTGCCGTACT CCACGTTTCG CACGTACGTC 
TTCAAGGAGA CCGAGTTCAT CGCCGTCACA GCATATCAAA ATGAGAAGAT AACTCAATTG 
AAAATCGATA ACAATCCGTT TGCGAAGGGC TTTCGTGATA CTGGTGCCGG CAAGCGGGAA 
AAGAAGCAGG CACTGATGTC GAACCGAGGG TCCGATTCGG ACAAGTTGAA TCCGACGCAT 
GTGAGCAGCT CGCGGGCACC GCTCCACCTG GGCCACGCCG GCCGTCCGCC TCATCTGCAT 
CCCCATGCCG CCCTGCTTGA TAATCAGCAG GACGACGACG ACAAGCTCCT GGACGTGGTG 
GGTCCGCCAC AGAGTCCGCT ACTCCCGCTC AGCCACTCGC TGCAGCAGAT GCACGCCCAC 
CAGCACTCCG CAGCTCTGGC TGCCTGGTTT AATCACTTGG CTGGCGCTGG AGCCGGAGCC 
TCCGAACATG CAGCTGCGGC GGCGGCCAAT GCCAGTGCGG AGGATGCACT GCGTCGTCGC 
TTGCAGGCGG ACGCGGATGT GGAGCGGGAT GGCAGCGATT CGAGTTGCTC GGAAAGCGTC 
GGCGGCAGTA CCGGCGGTGC CTTTAGGCCC ACCTCGACGG GCAGTCCCAA GGAGGCGGTG 
GGCGCGGCTG CAGCTGCCGC AGCGGCTGGC TTGAATCCCG GTGGCGGTAG CTATCCGTCA 
CCGAATATAT CGGTGGGTCC GCCCATTCAC CCGTCGCCGC ATTTGTTGCC TTACCTGTAT 
CCACATGGCC TGTATCCACC GCCGCATCTG GGCCTGCTGC ACAATCCCGC TGCAGCGGCG 
GCCATGAGTC CGGCTGGCCT GAATCCGGGT CTGCTCTTCA ATGCCCAACT GGCGCTGGCC 
GCCCAGCATC CGGCTTTGTT TGGCCACGCC TACGCGGCGG CGGGTCACAC ACCGGTATCA 
CCATTACAAG GCCTAAAGAG CCATCGCTTT TCGCCGTACA GTTTGCCCGG CAGTTTGGGC 
TCCGCCTTCG ATGCAGTGAC TCCTGGATCG AACGCAAATC GCTCGGGAGA TCCTCCAGGC 
GGCGGCGGCG GCGGCCTGGG TGGTGGTGTG GTGGAGAATG GTCCCAGGAG TCTGAGTTCC 
AGCCCTCGTC CCCGACCTGC CTCCCACTCG CCGCCCACTC GACCGATTTC GATGTCACCC 
ACAACGCCGC CATCCCTGAT GAAGCAACCA CGTGGCGGTG GTGCTGGCGC CGGTGTGGCC 
CAATCGCAGC ACTCGCCTTC GGAACTTAAG AGCATGGAGA AGATGGTCAA CGGACTGGAG 
GTTCAACACA ATGGCAGTGC GGCGGCAGCA GCGGCGGCTC TTCAGCTGGC CGAGGAAGCT 
GCCCAGCATC ATCATCACAC CCAGGCGCAC CACCAGCAGC AGCAGCATCA GTCGCACCAC 
CAGCAACAGC ACCACCAGCA ACCGGCACAA CCACATCCGC ACCACCAGAC CCATCTACAC 
TCGCACCATG GGGCGACAAC GGGCGGTACG GATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162663.1
CDS	643..3561
Translation	MRYDVQELLFHQSAEDPFARFANGMAYHPFLQLTQRPTDFSVSSLLTAGSNNNNSGNTNSGNNNSNSNNNTNSNTNNTNNLVAVSPTGGGAQLSPQSNHSSSNTTTTSNTNNSSSNNNNNNSTHNNNNNHTNNNNNNNNNTSQKQGHHLSTTEEPPSPAGTPPPTIVGLPPIPPPNNNSSSSSSNNSASAAAHPSHHPTAAHHSPSTGAAAPPAGATGLPPPTPPHHLQQQQQQQQHPAPPPPPYFPAAALAALAGSPAGPHPGLYPGGGLRFPPHHPGAHPHAHHLGSAYTTAEDVVLASAVAHQLHPAMRPLRALQPEDDGVVDDPKVTLEGKDLWEKFHKLGTEMVITKSGRQMFPQMKFRVSGLDAKAKYILLLDIVAADDYRYKFHNSRWMVAGKADPEMPKRMYIHPDSPTTGEQWMQKVVSFHKLKLTNNISDKHGFVSTTILNSMHKYQPRFHLVRANDILKLPYSTFRTYVFKETEFIAVTAYQNEKITQLKIDNNPFAKGFRDTGAGKREKKQALMSNRGSDSDKLNPTHVSSSRAPLHLGHAGRPPHLHPHAALLDNQQDDDDKLLDVVGPPQSPLLPLSHSLQQMHAHQHSAALAAWFNHLAGAGAGASEHAAAAAANASAEDALRRRLQADADVERDGSDSSCSESVGGSTGGAFRPTSTGSPKEAVGAAAAAAAAGLNPGGGSYPSPNISVGPPIHPSPHLLPYLYPHGLYPPPHLGLLHNPAAAAAMSPAGLNPGLLFNAQLALAAQHPALFGHAYAAAGHTPVSPLQGLKSHRFSPYSLPGSLGSAFDAVTPGSNANRSGDPPGGGGGGLGGGVVENGPRSLSSSPRPRPASHSPPTRPISMSPTTPPSLMKQPRGGGAGAGVAQSQHSPSELKSMEKMVNGLEVQHNGSAAAAAAALQLAEEAAQHHHHTQAHHQQQQHQSHHQQQHHQQPAQPHPHHQTHLHSHHGATTGGTDQ

Target ORF information:

RefSeq Version	NM_001169192.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bifid (bi), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169192.2

ORF Insert Sequence:

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTGTAAGTAC TACGATCCTG AACTCGATGC ACAAGTACCA GCCGCGTTTC 
CACCTGGTGC GAGCCAATGA CATCCTGAAG CTGCCGTACT CCACGTTTCG CACGTACGTC 
TTCAAGGAGA CCGAGTTCAT CGCCGTCACA GCATATCAAA ATGAGAAGAT AACTCAATTG 
AAAATCGATA ACAATCCGTT TGCGAAGGGC TTTCGTGATA CTGGTGCCGG CAAGCGGGAA 
AAGAAGCAGG CACTGATGTC GAACCGAGGG TCCGATTCGG ACAAGTTGAA TCCGACGCAT 
GTGAGCAGCT CGCGGGCACC GCTCCACCTG GGCCACGCCG GCCGTCCGCC TCATCTGCAT 
CCCCATGCCG CCCTGCTTGA TAATCAGCAG GACGACGACG ACAAGCTCCT GGACGTGGTG 
GGTCCGCCAC AGAGTCCGCT ACTCCCGCTC AGCCACTCGC TGCAGCAGAT GCACGCCCAC 
CAGCACTCCG CAGCTCTGGC TGCCTGGTTT AATCACTTGG CTGGCGCTGG AGCCGGAGCC 
TCCGAACATG CAGCTGCGGC GGCGGCCAAT GCCAGTGCGG AGGATGCACT GCGTCGTCGC 
TTGCAGGCGG ACGCGGATGT GGAGCGGGAT GGCAGCGATT CGAGTTGCTC GGAAAGCGTC 
GGCGGCAGTA CCGGCGGTGC CTTTAGGCCC ACCTCGACGG GCAGTCCCAA GGAGGCGGTG 
GGCGCGGCTG CAGCTGCCGC AGCGGCTGGC TTGAATCCCG GTGGCGGTAG CTATCCGTCA 
CCGAATATAT CGGTGGGTCC GCCCATTCAC CCGTCGCCGC ATTTGTTGCC TTACCTGTAT 
CCACATGGCC TGTATCCACC GCCGCATCTG GGCCTGCTGC ACAATCCCGC TGCAGCGGCG 
GCCATGAGTC CGGCTGGCCT GAATCCGGGT CTGCTCTTCA ATGCCCAACT GGCGCTGGCC 
GCCCAGCATC CGGCTTTGTT TGGCCACGCC TACGCGGCGG CGGGTCACAC ACCGGTATCA 
CCATTACAAG GCCTAAAGAG CCATCGCTTT TCGCCGTACA GTTTGCCCGG CAGTTTGGGC 
TCCGCCTTCG ATGCAGTGAC TCCTGGATCG AACGCAAATC GCTCGGGAGA TCCTCCAGGC 
GGCGGCGGCG GCGGCCTGGG TGGTGGTGTG GTGGAGAATG GTCCCAGGAG TCTGAGTTCC 
AGCCCTCGTC CCCGACCTGC CTCCCACTCG CCGCCCACTC GACCGATTTC GATGTCACCC 
ACAACGCCGC CATCCCTGAT GAAGCAACCA CGTGGCGGTG GTGCTGGCGC CGGTGTGGCC 
CAATCGCAGC ACTCGCCTTC GGAACTTAAG AGCATGGAGA AGATGGTCAA CGGACTGGAG 
GTTCAACACA ATGGCAGTGC GGCGGCAGCA GCGGCGGCTC TTCAGCTGGC CGAGGAAGCT 
GCCCAGCATC ATCATCACAC CCAGGCGCAC CACCAGCAGC AGCAGCATCA GTCGCACCAC 
CAGCAACAGC ACCACCAGCA ACCGGCACAA CCACATCCGC ACCACCAGAC CCATCTACAC 
TCGCACCATG GGGCGACAAC GGGCGGTACG GATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bi ( NM_001169191.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162662.1 Drosophila melanogaster bifid (bi), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00775
Clone ID Related Accession (Same CDS sequence)	NM_080331.3 , NM_001169192.2 , NM_001169191.1
Accession Version	NM_001169191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bifid, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTGTAAGTAC TACGATCCTG AACTCGATGC ACAAGTACCA GCCGCGTTTC 
CACCTGGTGC GAGCCAATGA CATCCTGAAG CTGCCGTACT CCACGTTTCG CACGTACGTC 
TTCAAGGAGA CCGAGTTCAT CGCCGTCACA GCATATCAAA ATGAGAAGAT AACTCAATTG 
AAAATCGATA ACAATCCGTT TGCGAAGGGC TTTCGTGATA CTGGTGCCGG CAAGCGGGAA 
AAGAAGCAGG CACTGATGTC GAACCGAGGG TCCGATTCGG ACAAGTTGAA TCCGACGCAT 
GTGAGCAGCT CGCGGGCACC GCTCCACCTG GGCCACGCCG GCCGTCCGCC TCATCTGCAT 
CCCCATGCCG CCCTGCTTGA TAATCAGCAG GACGACGACG ACAAGCTCCT GGACGTGGTG 
GGTCCGCCAC AGAGTCCGCT ACTCCCGCTC AGCCACTCGC TGCAGCAGAT GCACGCCCAC 
CAGCACTCCG CAGCTCTGGC TGCCTGGTTT AATCACTTGG CTGGCGCTGG AGCCGGAGCC 
TCCGAACATG CAGCTGCGGC GGCGGCCAAT GCCAGTGCGG AGGATGCACT GCGTCGTCGC 
TTGCAGGCGG ACGCGGATGT GGAGCGGGAT GGCAGCGATT CGAGTTGCTC GGAAAGCGTC 
GGCGGCAGTA CCGGCGGTGC CTTTAGGCCC ACCTCGACGG GCAGTCCCAA GGAGGCGGTG 
GGCGCGGCTG CAGCTGCCGC AGCGGCTGGC TTGAATCCCG GTGGCGGTAG CTATCCGTCA 
CCGAATATAT CGGTGGGTCC GCCCATTCAC CCGTCGCCGC ATTTGTTGCC TTACCTGTAT 
CCACATGGCC TGTATCCACC GCCGCATCTG GGCCTGCTGC ACAATCCCGC TGCAGCGGCG 
GCCATGAGTC CGGCTGGCCT GAATCCGGGT CTGCTCTTCA ATGCCCAACT GGCGCTGGCC 
GCCCAGCATC CGGCTTTGTT TGGCCACGCC TACGCGGCGG CGGGTCACAC ACCGGTATCA 
CCATTACAAG GCCTAAAGAG CCATCGCTTT TCGCCGTACA GTTTGCCCGG CAGTTTGGGC 
TCCGCCTTCG ATGCAGTGAC TCCTGGATCG AACGCAAATC GCTCGGGAGA TCCTCCAGGC 
GGCGGCGGCG GCGGCCTGGG TGGTGGTGTG GTGGAGAATG GTCCCAGGAG TCTGAGTTCC 
AGCCCTCGTC CCCGACCTGC CTCCCACTCG CCGCCCACTC GACCGATTTC GATGTCACCC 
ACAACGCCGC CATCCCTGAT GAAGCAACCA CGTGGCGGTG GTGCTGGCGC CGGTGTGGCC 
CAATCGCAGC ACTCGCCTTC GGAACTTAAG AGCATGGAGA AGATGGTCAA CGGACTGGAG 
GTTCAACACA ATGGCAGTGC GGCGGCAGCA GCGGCGGCTC TTCAGCTGGC CGAGGAAGCT 
GCCCAGCATC ATCATCACAC CCAGGCGCAC CACCAGCAGC AGCAGCATCA GTCGCACCAC 
CAGCAACAGC ACCACCAGCA ACCGGCACAA CCACATCCGC ACCACCAGAC CCATCTACAC 
TCGCACCATG GGGCGACAAC GGGCGGTACG GATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162662.1
CDS	676..3594
Translation	MRYDVQELLFHQSAEDPFARFANGMAYHPFLQLTQRPTDFSVSSLLTAGSNNNNSGNTNSGNNNSNSNNNTNSNTNNTNNLVAVSPTGGGAQLSPQSNHSSSNTTTTSNTNNSSSNNNNNNSTHNNNNNHTNNNNNNNNNTSQKQGHHLSTTEEPPSPAGTPPPTIVGLPPIPPPNNNSSSSSSNNSASAAAHPSHHPTAAHHSPSTGAAAPPAGATGLPPPTPPHHLQQQQQQQQHPAPPPPPYFPAAALAALAGSPAGPHPGLYPGGGLRFPPHHPGAHPHAHHLGSAYTTAEDVVLASAVAHQLHPAMRPLRALQPEDDGVVDDPKVTLEGKDLWEKFHKLGTEMVITKSGRQMFPQMKFRVSGLDAKAKYILLLDIVAADDYRYKFHNSRWMVAGKADPEMPKRMYIHPDSPTTGEQWMQKVVSFHKLKLTNNISDKHGFVSTTILNSMHKYQPRFHLVRANDILKLPYSTFRTYVFKETEFIAVTAYQNEKITQLKIDNNPFAKGFRDTGAGKREKKQALMSNRGSDSDKLNPTHVSSSRAPLHLGHAGRPPHLHPHAALLDNQQDDDDKLLDVVGPPQSPLLPLSHSLQQMHAHQHSAALAAWFNHLAGAGAGASEHAAAAAANASAEDALRRRLQADADVERDGSDSSCSESVGGSTGGAFRPTSTGSPKEAVGAAAAAAAAGLNPGGGSYPSPNISVGPPIHPSPHLLPYLYPHGLYPPPHLGLLHNPAAAAAMSPAGLNPGLLFNAQLALAAQHPALFGHAYAAAGHTPVSPLQGLKSHRFSPYSLPGSLGSAFDAVTPGSNANRSGDPPGGGGGGLGGGVVENGPRSLSSSPRPRPASHSPPTRPISMSPTTPPSLMKQPRGGGAGAGVAQSQHSPSELKSMEKMVNGLEVQHNGSAAAAAAALQLAEEAAQHHHHTQAHHQQQQHQSHHQQQHHQQPAQPHPHHQTHLHSHHGATTGGTDQ

Target ORF information:

RefSeq Version	NM_001169191.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bifid (bi), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169191.1

ORF Insert Sequence:

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTGTAAGTAC TACGATCCTG AACTCGATGC ACAAGTACCA GCCGCGTTTC 
CACCTGGTGC GAGCCAATGA CATCCTGAAG CTGCCGTACT CCACGTTTCG CACGTACGTC 
TTCAAGGAGA CCGAGTTCAT CGCCGTCACA GCATATCAAA ATGAGAAGAT AACTCAATTG 
AAAATCGATA ACAATCCGTT TGCGAAGGGC TTTCGTGATA CTGGTGCCGG CAAGCGGGAA 
AAGAAGCAGG CACTGATGTC GAACCGAGGG TCCGATTCGG ACAAGTTGAA TCCGACGCAT 
GTGAGCAGCT CGCGGGCACC GCTCCACCTG GGCCACGCCG GCCGTCCGCC TCATCTGCAT 
CCCCATGCCG CCCTGCTTGA TAATCAGCAG GACGACGACG ACAAGCTCCT GGACGTGGTG 
GGTCCGCCAC AGAGTCCGCT ACTCCCGCTC AGCCACTCGC TGCAGCAGAT GCACGCCCAC 
CAGCACTCCG CAGCTCTGGC TGCCTGGTTT AATCACTTGG CTGGCGCTGG AGCCGGAGCC 
TCCGAACATG CAGCTGCGGC GGCGGCCAAT GCCAGTGCGG AGGATGCACT GCGTCGTCGC 
TTGCAGGCGG ACGCGGATGT GGAGCGGGAT GGCAGCGATT CGAGTTGCTC GGAAAGCGTC 
GGCGGCAGTA CCGGCGGTGC CTTTAGGCCC ACCTCGACGG GCAGTCCCAA GGAGGCGGTG 
GGCGCGGCTG CAGCTGCCGC AGCGGCTGGC TTGAATCCCG GTGGCGGTAG CTATCCGTCA 
CCGAATATAT CGGTGGGTCC GCCCATTCAC CCGTCGCCGC ATTTGTTGCC TTACCTGTAT 
CCACATGGCC TGTATCCACC GCCGCATCTG GGCCTGCTGC ACAATCCCGC TGCAGCGGCG 
GCCATGAGTC CGGCTGGCCT GAATCCGGGT CTGCTCTTCA ATGCCCAACT GGCGCTGGCC 
GCCCAGCATC CGGCTTTGTT TGGCCACGCC TACGCGGCGG CGGGTCACAC ACCGGTATCA 
CCATTACAAG GCCTAAAGAG CCATCGCTTT TCGCCGTACA GTTTGCCCGG CAGTTTGGGC 
TCCGCCTTCG ATGCAGTGAC TCCTGGATCG AACGCAAATC GCTCGGGAGA TCCTCCAGGC 
GGCGGCGGCG GCGGCCTGGG TGGTGGTGTG GTGGAGAATG GTCCCAGGAG TCTGAGTTCC 
AGCCCTCGTC CCCGACCTGC CTCCCACTCG CCGCCCACTC GACCGATTTC GATGTCACCC 
ACAACGCCGC CATCCCTGAT GAAGCAACCA CGTGGCGGTG GTGCTGGCGC CGGTGTGGCC 
CAATCGCAGC ACTCGCCTTC GGAACTTAAG AGCATGGAGA AGATGGTCAA CGGACTGGAG 
GTTCAACACA ATGGCAGTGC GGCGGCAGCA GCGGCGGCTC TTCAGCTGGC CGAGGAAGCT 
GCCCAGCATC ATCATCACAC CCAGGCGCAC CACCAGCAGC AGCAGCATCA GTCGCACCAC 
CAGCAACAGC ACCACCAGCA ACCGGCACAA CCACATCCGC ACCACCAGAC CCATCTACAC 
TCGCACCATG GGGCGACAAC GGGCGGTACG GATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bi ( NM_001272309.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259238.1 Drosophila melanogaster bifid (bi), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa03755
Clone ID Related Accession (Same CDS sequence)	NM_001272309.2
Accession Version	NM_001272309.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bifid, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272309.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTGTAAGTAC TACGATCCTG AACTCGATGC ACAAGTACCA GCCGCGTTTC 
CACCTGGTGC GAGCCAATGA CATCCTGAAG CTGCCGTACT CCACGTTTCG CACGTACGTC 
TTCAAGGAGA CCGAGTTCAT CGCCGTCACA GCATATCAAA ATGAGAAGAT AACTCAATTG 
AAAATCGATA ACAATCCGTT TGCGAAGGGC TTTCGTGATA CTGGTGCCGG CAAGCGGGAA 
AAGAAGCAGG CACTGATGTC GAACCGAGGG TCCGATTCGG ACAAGTTGAA TCCGACGCAT 
GTGAGCAGCT CGCGGGCACC GCTCCACCTG GGCCACGCCG GCCGTCCGCC TCATCTGCAT 
CCCCATGCCG CCCTGCTTGA TAATCAGCAG GACGACGACG ACAAGCTCCT GGACGTGGTG 
GGTCCGCCAC AGAGTCCGCT ACTCCCGCTC AGCCACTCGC TGCAGCAGAT GCACGCCCAC 
CAGCACTCCG CAGCTCTGGC TGCCTGGTTT AATCACTTGG CTGGCGCTGG AGCCGGAGCC 
TCCGAACATG CAGCTGCGGC GGCGGCCAAT GCCAGTGCGG AGGATGCACT GCGTCGTCGC 
TTGCAGGCGG ACGCGGATGT GGAGCGGGAT GGCAGCGATT CGAGTTGCTC GGAAAGCGTC 
GGCGGCAGTA CCGGCGGTGC CTTTAGGCCC ACCTCGACGG GCAGTCCCAA GGAGGCGGTG 
GGCGCGGCTG CAGCTGCCGC AGCGGCTGGC TTGAATCCCG GTGGCGGTAG CTATCCGTCA 
CCGAATATAT CGGTGGGTCC GCCCATTCAC CCGTCGCCGC ATTTGTTGCC TTACCTGTAT 
CCACATGGCC TGTATCCACC GCCGCATCTG GGCCTGCTGC ACAATCCCGC TGCAGCGGCG 
GCCATGAGTC CGGCTGGCCT GAATCCGGGT CTGCTCTTCA ATGCCCAACT GGCGCTGGCC 
GCCCAGCATC CGGCTTTGTT TGGCCACGCC TACGCGGCGG CGGGTCACAC ACCGGTATCA 
CCATTACAAG GCCTAAAGAG CCATCGCTTT TCGCCGTACA GTTTGCCCGG CAGTTTGGGC 
TCCGCCTTCG ATGCAGTGAC TCCTGGATCG AACGCAAATC GCTCGGGAGA TCCTCCAGGC 
GGCGGCGGCG GCGGCCTGGG TGGTGGTGTG GTGGAGAATG GTCCCAGGAG TCTGAGTTCC 
AGCCCTCGTC CCCGACCTGC CTCCCACTCG CCGCCCACTC GACCGATTTC GATGTCACCC 
ACAACGCCGC CATCCCTGAT GAAGCAACCA CGTGGCGGTG GTGCTGGCGC CGGTGTGGCC 
CAATCGCAGC ACTCGCCTTC GGAACTTAAG AGCATGGAGA AGATGGTCAA CGGACTGGAG 
GTTCAACACA ATGGCAGTGC GGCGGCAGCA GCGGCGGCTC TTCAGCTGGC CGAGGAAGCT 
GCCCAGCATC ATCATCACAC CCAGGCGCAC CACCAGCAGC AGCAGCATCA GTCGCACCAC 
CAGCAACAGC ACCACCAGCA ACCGGCACAA CCACATCCGC ACCACCAGAC CCATCTACAC 
TCGCACCATG GGGCGACAAC GGGCGGTACG GATCAGTGAC AATTACTGGA CGGCCGCTCC 
GATTGTGAGG ATGCCGGTCT CGAATTGGAA CTAGAGTTGG AGGAGGATGT CGAGGATCTG 
GATCAGGATC CGGATGAGCA GGACGAGGAT CGCAGCTCGG TCATCGATCT TGATCTGGAC 
GTCGACGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259238.1
CDS	676..3747
Translation	MRYDVQELLFHQSAEDPFARFANGMAYHPFLQLTQRPTDFSVSSLLTAGSNNNNSGNTNSGNNNSNSNNNTNSNTNNTNNLVAVSPTGGGAQLSPQSNHSSSNTTTTSNTNNSSSNNNNNNSTHNNNNNHTNNNNNNNNNTSQKQGHHLSTTEEPPSPAGTPPPTIVGLPPIPPPNNNSSSSSSNNSASAAAHPSHHPTAAHHSPSTGAAAPPAGATGLPPPTPPHHLQQQQQQQQHPAPPPPPYFPAAALAALAGSPAGPHPGLYPGGGLRFPPHHPGAHPHAHHLGSAYTTAEDVVLASAVAHQLHPAMRPLRALQPEDDGVVDDPKVTLEGKDLWEKFHKLGTEMVITKSGRQMFPQMKFRVSGLDAKAKYILLLDIVAADDYRYKFHNSRWMVAGKADPEMPKRMYIHPDSPTTGEQWMQKVVSFHKLKLTNNISDKHGFVSTTILNSMHKYQPRFHLVRANDILKLPYSTFRTYVFKETEFIAVTAYQNEKITQLKIDNNPFAKGFRDTGAGKREKKQALMSNRGSDSDKLNPTHVSSSRAPLHLGHAGRPPHLHPHAALLDNQQDDDDKLLDVVGPPQSPLLPLSHSLQQMHAHQHSAALAAWFNHLAGAGAGASEHAAAAAANASAEDALRRRLQADADVERDGSDSSCSESVGGSTGGAFRPTSTGSPKEAVGAAAAAAAAGLNPGGGSYPSPNISVGPPIHPSPHLLPYLYPHGLYPPPHLGLLHNPAAAAAMSPAGLNPGLLFNAQLALAAQHPALFGHAYAAAGHTPVSPLQGLKSHRFSPYSLPGSLGSAFDAVTPGSNANRSGDPPGGGGGGLGGGVVENGPRSLSSSPRPRPASHSPPTRPISMSPTTPPSLMKQPRGGGAGAGVAQSQHSPSELKSMEKMVNGLEVQHNGSAAAAAAALQLAEEAAQHHHHTQAHHQQQQHQSHHQQQHHQQPAQPHPHHQTHLHSHHGATTGGTDQ*QLLDGRSDCEDAGLELELELEEDVEDLDQDPDEQDEDRSSVIDLDLDVDV

Target ORF information:

RefSeq Version	NM_001272309.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bifid (bi), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272309.2

ORF Insert Sequence:

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTGTAAGTAC TACGATCCTG AACTCGATGC ACAAGTACCA GCCGCGTTTC 
CACCTGGTGC GAGCCAATGA CATCCTGAAG CTGCCGTACT CCACGTTTCG CACGTACGTC 
TTCAAGGAGA CCGAGTTCAT CGCCGTCACA GCATATCAAA ATGAGAAGAT AACTCAATTG 
AAAATCGATA ACAATCCGTT TGCGAAGGGC TTTCGTGATA CTGGTGCCGG CAAGCGGGAA 
AAGAAGCAGG CACTGATGTC GAACCGAGGG TCCGATTCGG ACAAGTTGAA TCCGACGCAT 
GTGAGCAGCT CGCGGGCACC GCTCCACCTG GGCCACGCCG GCCGTCCGCC TCATCTGCAT 
CCCCATGCCG CCCTGCTTGA TAATCAGCAG GACGACGACG ACAAGCTCCT GGACGTGGTG 
GGTCCGCCAC AGAGTCCGCT ACTCCCGCTC AGCCACTCGC TGCAGCAGAT GCACGCCCAC 
CAGCACTCCG CAGCTCTGGC TGCCTGGTTT AATCACTTGG CTGGCGCTGG AGCCGGAGCC 
TCCGAACATG CAGCTGCGGC GGCGGCCAAT GCCAGTGCGG AGGATGCACT GCGTCGTCGC 
TTGCAGGCGG ACGCGGATGT GGAGCGGGAT GGCAGCGATT CGAGTTGCTC GGAAAGCGTC 
GGCGGCAGTA CCGGCGGTGC CTTTAGGCCC ACCTCGACGG GCAGTCCCAA GGAGGCGGTG 
GGCGCGGCTG CAGCTGCCGC AGCGGCTGGC TTGAATCCCG GTGGCGGTAG CTATCCGTCA 
CCGAATATAT CGGTGGGTCC GCCCATTCAC CCGTCGCCGC ATTTGTTGCC TTACCTGTAT 
CCACATGGCC TGTATCCACC GCCGCATCTG GGCCTGCTGC ACAATCCCGC TGCAGCGGCG 
GCCATGAGTC CGGCTGGCCT GAATCCGGGT CTGCTCTTCA ATGCCCAACT GGCGCTGGCC 
GCCCAGCATC CGGCTTTGTT TGGCCACGCC TACGCGGCGG CGGGTCACAC ACCGGTATCA 
CCATTACAAG GCCTAAAGAG CCATCGCTTT TCGCCGTACA GTTTGCCCGG CAGTTTGGGC 
TCCGCCTTCG ATGCAGTGAC TCCTGGATCG AACGCAAATC GCTCGGGAGA TCCTCCAGGC 
GGCGGCGGCG GCGGCCTGGG TGGTGGTGTG GTGGAGAATG GTCCCAGGAG TCTGAGTTCC 
AGCCCTCGTC CCCGACCTGC CTCCCACTCG CCGCCCACTC GACCGATTTC GATGTCACCC 
ACAACGCCGC CATCCCTGAT GAAGCAACCA CGTGGCGGTG GTGCTGGCGC CGGTGTGGCC 
CAATCGCAGC ACTCGCCTTC GGAACTTAAG AGCATGGAGA AGATGGTCAA CGGACTGGAG 
GTTCAACACA ATGGCAGTGC GGCGGCAGCA GCGGCGGCTC TTCAGCTGGC CGAGGAAGCT 
GCCCAGCATC ATCATCACAC CCAGGCGCAC CACCAGCAGC AGCAGCATCA GTCGCACCAC 
CAGCAACAGC ACCACCAGCA ACCGGCACAA CCACATCCGC ACCACCAGAC CCATCTACAC 
TCGCACCATG GGGCGACAAC GGGCGGTACG GATCAGTGAC AATTACTGGA CGGCCGCTCC 
GATTGTGAGG ATGCCGGTCT CGAATTGGAA CTAGAGTTGG AGGAGGATGT CGAGGATCTG 
GATCAGGATC CGGATGAGCA GGACGAGGAT CGCAGCTCGG TCATCGATCT TGATCTGGAC 
GTCGACGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bi ( NM_001258599.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245528.1 Drosophila melanogaster bifid (bi), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa03756
Clone ID Related Accession (Same CDS sequence)	NM_001258599.2
Accession Version	NM_001258599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bifid, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001258599.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTACGATCCT GAACTCGATG CACAAGTACC AGCCGCGTTT CCACCTGGTG 
CGAGCCAATG ACATCCTGAA GCTGCCGTAC TCCACGTTTC GCACGTACGT CTTCAAGGAG 
ACCGAGTTCA TCGCCGTCAC AGCATATCAA AATGAGAAGA TAACTCAATT GAAAATCGAT 
AACAATCCGT TTGCGAAGGG CTTTCGTGAT ACTGGTGCCG GCAAGCGGGA AAAGAATTAC 
AGGCAGGCAC TGATGTCGAA CCGAGGGTCC GATTCGGACA AGTTGAATCC GACGCATGTG 
AGCAGCTCGC GGGCACCGCT CCACCTGGGC CACGCCGGCC GTCCGCCTCA TCTGCATCCC 
CATGCCGCCC TGCTTGATAA TCAGCAGGAC GACGACGACA AGCTCCTGGA CGTGGTGGGT 
CCGCCACAGA GTCCGCTACT CCCGCTCAGC CACTCGCTGC AGCAGATGCA CGCCCACCAG 
CACTCCGCTC TGGCTGCCTG GTTTAATCAC TTGGCTGGCG CTGGAGCCGG AGCCTCCGAA 
CATGCAGCTG CGGCGGCGGC CAATGCCAGT GCGGAGGATG CACTGCGTCG TCGCTTGCAG 
GCGGACGCGG ATGTGGAGCG GGATGGCAGC GATTCGAGTT GCTCGGAAAG CGTCGGCGGC 
AGTACCGGCG GTGCCTTTAG GCCCACCTCG ACGGGCAGTC CCAAGGAGGC GGTGGGCGCG 
GCTGCAGCTG CCGCAGCGGC TGGCTTGAAT CCCGGTGGCG GTAGCTATCC GTCACCGAAT 
ATATCGGTGG GTCCGCCCAT TCACCCGTCG CCGCATTTGT TGCCTTACCT GTATCCACAT 
GGCCTGTATC CACCGCCGCA TCTGGGCCTG CTGCACAATC CCGCTGCAGC GGCGGCCATG 
AGTCCGGCTG GCCTGAATCC GGGTCTGCTC TTCAATGCCC AACTGGCGCT GGCCGCCCAG 
CATCCGGCTT TGTTTGGCCA CGCCTACGCG GCGGCGGGTC ACACACCGGT ATCACCATTA 
CAAGGCCTAA AGAGCCATCG CTTTTCGCCG TACAGTTTGC CCGGCAGTTT GGGCTCCGCC 
TTCGATGCAG TGACTCCTGG ATCGAACGCA AATCGCTCGG GAGATCCTCC AGGCGGCGGC 
GGCGGCGGCC TGGGTGGTGG TGTGGTGGAG AATGGTCCCA GGAGTCTGAG TTCCAGCCCT 
CGTCCCCGAC CTGCCTCCCA CTCGCCGCCC ACTCGACCGA TTTCGATGTC ACCCACAACG 
CCGCCATCCC TGATGAAGCA ACCACGTGGC GGTGGTGCTG GCGCCGGTGT GGCCCAATCG 
CAGCACTCGC CTTCGGAACT TAAGAGCATG GAGAAGATGG TCAACGGACT GGAGGTTCAA 
CACAATGGCA GTGCGGCGGC AGCAGCGGCG GCTCTTCAGC TGGCCGAGGA AGCTGCCCAG 
CATCATCATC ACACCCAGGC GCACCACCAG CAGCAGCAGC ATCAGTCGCA CCACCAGCAA 
CAGCACCACC AGCAACCGGC ACAACCACAT CCGCACCACC AGACCCATCT ACACTCGCAC 
CATGGGGCGA CAACGGGCGG TACGGATCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245528.1
CDS	456..3368
Translation	MRYDVQELLFHQSAEDPFARFANGMAYHPFLQLTQRPTDFSVSSLLTAGSNNNNSGNTNSGNNNSNSNNNTNSNTNNTNNLVAVSPTGGGAQLSPQSNHSSSNTTTTSNTNNSSSNNNNNNSTHNNNNNHTNNNNNNNNNTSQKQGHHLSTTEEPPSPAGTPPPTIVGLPPIPPPNNNSSSSSSNNSASAAAHPSHHPTAAHHSPSTGAAAPPAGATGLPPPTPPHHLQQQQQQQQHPAPPPPPYFPAAALAALAGSPAGPHPGLYPGGGLRFPPHHPGAHPHAHHLGSAYTTAEDVVLASAVAHQLHPAMRPLRALQPEDDGVVDDPKVTLEGKDLWEKFHKLGTEMVITKSGRQMFPQMKFRVSGLDAKAKYILLLDIVAADDYRYKFHNSRWMVAGKADPEMPKRMYIHPDSPTTGEQWMQKVVSFHKLKLTNNISDKHGFTILNSMHKYQPRFHLVRANDILKLPYSTFRTYVFKETEFIAVTAYQNEKITQLKIDNNPFAKGFRDTGAGKREKNYRQALMSNRGSDSDKLNPTHVSSSRAPLHLGHAGRPPHLHPHAALLDNQQDDDDKLLDVVGPPQSPLLPLSHSLQQMHAHQHSALAAWFNHLAGAGAGASEHAAAAAANASAEDALRRRLQADADVERDGSDSSCSESVGGSTGGAFRPTSTGSPKEAVGAAAAAAAAGLNPGGGSYPSPNISVGPPIHPSPHLLPYLYPHGLYPPPHLGLLHNPAAAAAMSPAGLNPGLLFNAQLALAAQHPALFGHAYAAAGHTPVSPLQGLKSHRFSPYSLPGSLGSAFDAVTPGSNANRSGDPPGGGGGGLGGGVVENGPRSLSSSPRPRPASHSPPTRPISMSPTTPPSLMKQPRGGGAGAGVAQSQHSPSELKSMEKMVNGLEVQHNGSAAAAAAALQLAEEAAQHHHHTQAHHQQQQHQSHHQQQHHQQPAQPHPHHQTHLHSHHGATTGGTDQ

Target ORF information:

RefSeq Version	NM_001258599.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bifid (bi), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258599.2

ORF Insert Sequence:

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTACGATCCT GAACTCGATG CACAAGTACC AGCCGCGTTT CCACCTGGTG 
CGAGCCAATG ACATCCTGAA GCTGCCGTAC TCCACGTTTC GCACGTACGT CTTCAAGGAG 
ACCGAGTTCA TCGCCGTCAC AGCATATCAA AATGAGAAGA TAACTCAATT GAAAATCGAT 
AACAATCCGT TTGCGAAGGG CTTTCGTGAT ACTGGTGCCG GCAAGCGGGA AAAGAATTAC 
AGGCAGGCAC TGATGTCGAA CCGAGGGTCC GATTCGGACA AGTTGAATCC GACGCATGTG 
AGCAGCTCGC GGGCACCGCT CCACCTGGGC CACGCCGGCC GTCCGCCTCA TCTGCATCCC 
CATGCCGCCC TGCTTGATAA TCAGCAGGAC GACGACGACA AGCTCCTGGA CGTGGTGGGT 
CCGCCACAGA GTCCGCTACT CCCGCTCAGC CACTCGCTGC AGCAGATGCA CGCCCACCAG 
CACTCCGCTC TGGCTGCCTG GTTTAATCAC TTGGCTGGCG CTGGAGCCGG AGCCTCCGAA 
CATGCAGCTG CGGCGGCGGC CAATGCCAGT GCGGAGGATG CACTGCGTCG TCGCTTGCAG 
GCGGACGCGG ATGTGGAGCG GGATGGCAGC GATTCGAGTT GCTCGGAAAG CGTCGGCGGC 
AGTACCGGCG GTGCCTTTAG GCCCACCTCG ACGGGCAGTC CCAAGGAGGC GGTGGGCGCG 
GCTGCAGCTG CCGCAGCGGC TGGCTTGAAT CCCGGTGGCG GTAGCTATCC GTCACCGAAT 
ATATCGGTGG GTCCGCCCAT TCACCCGTCG CCGCATTTGT TGCCTTACCT GTATCCACAT 
GGCCTGTATC CACCGCCGCA TCTGGGCCTG CTGCACAATC CCGCTGCAGC GGCGGCCATG 
AGTCCGGCTG GCCTGAATCC GGGTCTGCTC TTCAATGCCC AACTGGCGCT GGCCGCCCAG 
CATCCGGCTT TGTTTGGCCA CGCCTACGCG GCGGCGGGTC ACACACCGGT ATCACCATTA 
CAAGGCCTAA AGAGCCATCG CTTTTCGCCG TACAGTTTGC CCGGCAGTTT GGGCTCCGCC 
TTCGATGCAG TGACTCCTGG ATCGAACGCA AATCGCTCGG GAGATCCTCC AGGCGGCGGC 
GGCGGCGGCC TGGGTGGTGG TGTGGTGGAG AATGGTCCCA GGAGTCTGAG TTCCAGCCCT 
CGTCCCCGAC CTGCCTCCCA CTCGCCGCCC ACTCGACCGA TTTCGATGTC ACCCACAACG 
CCGCCATCCC TGATGAAGCA ACCACGTGGC GGTGGTGCTG GCGCCGGTGT GGCCCAATCG 
CAGCACTCGC CTTCGGAACT TAAGAGCATG GAGAAGATGG TCAACGGACT GGAGGTTCAA 
CACAATGGCA GTGCGGCGGC AGCAGCGGCG GCTCTTCAGC TGGCCGAGGA AGCTGCCCAG 
CATCATCATC ACACCCAGGC GCACCACCAG CAGCAGCAGC ATCAGTCGCA CCACCAGCAA 
CAGCACCACC AGCAACCGGC ACAACCACAT CCGCACCACC AGACCCATCT ACACTCGCAC 
CATGGGGCGA CAACGGGCGG TACGGATCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bi ( NM_001258598.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245527.1 Drosophila melanogaster bifid (bi), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa03757
Clone ID Related Accession (Same CDS sequence)	NM_001258598.3
Accession Version	NM_001258598.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bifid, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258598.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTGTAAGTAC TACGATCCTG AACTCGATGC ACAAGTACCA GCCGCGTTTC 
CACCTGGTGC GAGCCAATGA CATCCTGAAG CTGCCGTACT CCACGTTTCG CACGTACGTC 
TTCAAGGAGA CCGAGTTCAT CGCCGTCACA GCATATCAAA ATGAGAAGAT AACTCAATTG 
AAAATCGATA ACAATCCGTT TGCGAAGGGC TTTCGTGATA CTGGTGCCGG CAAGCGGGAA 
AAGAATTGTT ACAGGCAGGC ACTGATGTCG AACCGAGGGT CCGATTCGGA CAAGTTGAAT 
CCGACGCATG TGAGCAGCTC GCGGGCACCG CTCCACCTGG GCCACGCCGG CCGTCCGCCT 
CATCTGCATC CCCATGCCGC CCTGCTTGAT AATCAGCAGG ACGACGACGA CAAGCTCCTG 
GACGTGGTGG GTCCGCCACA GAGTCCGCTA CTCCCGCTCA GCCACTCGCT GCAGCAGATG 
CACGCCCACC AGCACTCCGC TCTGGCTGCC TGGTTTAATC ACTTGGCTGG CGCTGGAGCC 
GGAGCCTCCG AACATGCAGC TGCGGCGGCG GCCAATGCCA GTGCGGAGGA TGCACTGCGT 
CGTCGCTTGC AGGCGGACGC GGATGTGGAG CGGGATGGCA GCGATTCGAG TTGCTCGGAA 
AGCGTCGGCG GCAGTACCGG CGGTGCCTTT AGGCCCACCT CGACGGGCAG TCCCAAGGAG 
GCGGTGGGCG CGGCTGCAGC TGCCGCAGCG GCTGGCTTGA ATCCCGGTGG CGGTAGCTAT 
CCGTCACCGA ATATATCGGT GGGTCCGCCC ATTCACCCGT CGCCGCATTT GTTGCCTTAC 
CTGTATCCAC ATGGCCTGTA TCCACCGCCG CATCTGGGCC TGCTGCACAA TCCCGCTGCA 
GCGGCGGCCA TGAGTCCGGC TGGCCTGAAT CCGGGTCTGC TCTTCAATGC CCAACTGGCG 
CTGGCCGCCC AGCATCCGGC TTTGTTTGGC CACGCCTACG CGGCGGCGGG TCACACACCG 
GTATCACCAT TACAAGGCCT AAAGAGCCAT CGCTTTTCGC CGTACAGTTT GCCCGGCAGT 
TTGGGCTCCG CCTTCGATGC AGTGACTCCT GGATCGAACG CAAATCGCTC GGGAGATCCT 
CCAGGCGGCG GCGGCGGCGG CCTGGGTGGT GGTGTGGTGG AGAATGGTCC CAGGAGTCTG 
AGTTCCAGCC CTCGTCCCCG ACCTGCCTCC CACTCGCCGC CCACTCGACC GATTTCGATG 
TCACCCACAA CGCCGCCATC CCTGATGAAG CAACCACGTG GCGGTGGTGC TGGCGCCGGT 
GTGGCCCAAT CGCAGCACTC GCCTTCGGAA CTTAAGAGCA TGGAGAAGAT GGTCAACGGA 
CTGGAGGTTC AACACAATGG CAGTGCGGCG GCAGCAGCGG CGGCTCTTCA GCTGGCCGAG 
GAAGCTGCCC AGCATCATCA TCACACCCAG GCGCACCACC AGCAGCAGCA GCATCAGTCG 
CACCACCAGC AACAGCACCA CCAGCAACCG GCACAACCAC ATCCGCACCA CCAGACCCAT 
CTACACTCGC ACCATGGGGC GACAACGGGC GGTACGGATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245527.1
CDS	456..3380
Translation	MRYDVQELLFHQSAEDPFARFANGMAYHPFLQLTQRPTDFSVSSLLTAGSNNNNSGNTNSGNNNSNSNNNTNSNTNNTNNLVAVSPTGGGAQLSPQSNHSSSNTTTTSNTNNSSSNNNNNNSTHNNNNNHTNNNNNNNNNTSQKQGHHLSTTEEPPSPAGTPPPTIVGLPPIPPPNNNSSSSSSNNSASAAAHPSHHPTAAHHSPSTGAAAPPAGATGLPPPTPPHHLQQQQQQQQHPAPPPPPYFPAAALAALAGSPAGPHPGLYPGGGLRFPPHHPGAHPHAHHLGSAYTTAEDVVLASAVAHQLHPAMRPLRALQPEDDGVVDDPKVTLEGKDLWEKFHKLGTEMVITKSGRQMFPQMKFRVSGLDAKAKYILLLDIVAADDYRYKFHNSRWMVAGKADPEMPKRMYIHPDSPTTGEQWMQKVVSFHKLKLTNNISDKHGFVSTTILNSMHKYQPRFHLVRANDILKLPYSTFRTYVFKETEFIAVTAYQNEKITQLKIDNNPFAKGFRDTGAGKREKNCYRQALMSNRGSDSDKLNPTHVSSSRAPLHLGHAGRPPHLHPHAALLDNQQDDDDKLLDVVGPPQSPLLPLSHSLQQMHAHQHSALAAWFNHLAGAGAGASEHAAAAAANASAEDALRRRLQADADVERDGSDSSCSESVGGSTGGAFRPTSTGSPKEAVGAAAAAAAAGLNPGGGSYPSPNISVGPPIHPSPHLLPYLYPHGLYPPPHLGLLHNPAAAAAMSPAGLNPGLLFNAQLALAAQHPALFGHAYAAAGHTPVSPLQGLKSHRFSPYSLPGSLGSAFDAVTPGSNANRSGDPPGGGGGGLGGGVVENGPRSLSSSPRPRPASHSPPTRPISMSPTTPPSLMKQPRGGGAGAGVAQSQHSPSELKSMEKMVNGLEVQHNGSAAAAAAALQLAEEAAQHHHHTQAHHQQQQHQSHHQQQHHQQPAQPHPHHQTHLHSHHGATTGGTDQ

Target ORF information:

RefSeq Version	NM_001258598.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bifid (bi), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258598.3

ORF Insert Sequence:

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTGTAAGTAC TACGATCCTG AACTCGATGC ACAAGTACCA GCCGCGTTTC 
CACCTGGTGC GAGCCAATGA CATCCTGAAG CTGCCGTACT CCACGTTTCG CACGTACGTC 
TTCAAGGAGA CCGAGTTCAT CGCCGTCACA GCATATCAAA ATGAGAAGAT AACTCAATTG 
AAAATCGATA ACAATCCGTT TGCGAAGGGC TTTCGTGATA CTGGTGCCGG CAAGCGGGAA 
AAGAATTGTT ACAGGCAGGC ACTGATGTCG AACCGAGGGT CCGATTCGGA CAAGTTGAAT 
CCGACGCATG TGAGCAGCTC GCGGGCACCG CTCCACCTGG GCCACGCCGG CCGTCCGCCT 
CATCTGCATC CCCATGCCGC CCTGCTTGAT AATCAGCAGG ACGACGACGA CAAGCTCCTG 
GACGTGGTGG GTCCGCCACA GAGTCCGCTA CTCCCGCTCA GCCACTCGCT GCAGCAGATG 
CACGCCCACC AGCACTCCGC TCTGGCTGCC TGGTTTAATC ACTTGGCTGG CGCTGGAGCC 
GGAGCCTCCG AACATGCAGC TGCGGCGGCG GCCAATGCCA GTGCGGAGGA TGCACTGCGT 
CGTCGCTTGC AGGCGGACGC GGATGTGGAG CGGGATGGCA GCGATTCGAG TTGCTCGGAA 
AGCGTCGGCG GCAGTACCGG CGGTGCCTTT AGGCCCACCT CGACGGGCAG TCCCAAGGAG 
GCGGTGGGCG CGGCTGCAGC TGCCGCAGCG GCTGGCTTGA ATCCCGGTGG CGGTAGCTAT 
CCGTCACCGA ATATATCGGT GGGTCCGCCC ATTCACCCGT CGCCGCATTT GTTGCCTTAC 
CTGTATCCAC ATGGCCTGTA TCCACCGCCG CATCTGGGCC TGCTGCACAA TCCCGCTGCA 
GCGGCGGCCA TGAGTCCGGC TGGCCTGAAT CCGGGTCTGC TCTTCAATGC CCAACTGGCG 
CTGGCCGCCC AGCATCCGGC TTTGTTTGGC CACGCCTACG CGGCGGCGGG TCACACACCG 
GTATCACCAT TACAAGGCCT AAAGAGCCAT CGCTTTTCGC CGTACAGTTT GCCCGGCAGT 
TTGGGCTCCG CCTTCGATGC AGTGACTCCT GGATCGAACG CAAATCGCTC GGGAGATCCT 
CCAGGCGGCG GCGGCGGCGG CCTGGGTGGT GGTGTGGTGG AGAATGGTCC CAGGAGTCTG 
AGTTCCAGCC CTCGTCCCCG ACCTGCCTCC CACTCGCCGC CCACTCGACC GATTTCGATG 
TCACCCACAA CGCCGCCATC CCTGATGAAG CAACCACGTG GCGGTGGTGC TGGCGCCGGT 
GTGGCCCAAT CGCAGCACTC GCCTTCGGAA CTTAAGAGCA TGGAGAAGAT GGTCAACGGA 
CTGGAGGTTC AACACAATGG CAGTGCGGCG GCAGCAGCGG CGGCTCTTCA GCTGGCCGAG 
GAAGCTGCCC AGCATCATCA TCACACCCAG GCGCACCACC AGCAGCAGCA GCATCAGTCG 
CACCACCAGC AACAGCACCA CCAGCAACCG GCACAACCAC ATCCGCACCA CCAGACCCAT 
CTACACTCGC ACCATGGGGC GACAACGGGC GGTACGGATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bi ( NM_001297932.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284861.1 Drosophila melanogaster bifid (bi), transcript variant G, mRNA.

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CloneID	OFa21357
Clone ID Related Accession (Same CDS sequence)	NM_001297932.1
Accession Version	NM_001297932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bifid, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTACGATCCT GAACTCGATG CACAAGTACC AGCCGCGTTT CCACCTGGTG 
CGAGCCAATG ACATCCTGAA GCTGCCGTAC TCCACGTTTC GCACGTACGT CTTCAAGGAG 
ACCGAGTTCA TCGCCGTCAC AGCATATCAA AATGAGAAGA TAACTCAATT GAAAATCGAT 
AACAATCCGT TTGCGAAGGG CTTTCGTGAT ACTGGTGCCG GCAAGCGGGA AAAGAATTGT 
TACAGGCAGG CACTGATGTC GAACCGAGGG TCCGATTCGG ACAAGTTGAA TCCGACGCAT 
GTGAGCAGCT CGCGGGCACC GCTCCACCTG GGCCACGCCG GCCGTCCGCC TCATCTGCAT 
CCCCATGCCG CCCTGCTTGA TAATCAGCAG GACGACGACG ACAAGCTCCT GGACGTGGTG 
GGTCCGCCAC AGAGTCCGCT ACTCCCGCTC AGCCACTCGC TGCAGCAGAT GCACGCCCAC 
CAGCACTCCG CTCTGGCTGC CTGGTTTAAT CACTTGGCTG GCGCTGGAGC CGGAGCCTCC 
GAACATGCAG CTGCGGCGGC GGCCAATGCC AGTGCGGAGG ATGCACTGCG TCGTCGCTTG 
CAGGCGGACG CGGATGTGGA GCGGGATGGC AGCGATTCGA GTTGCTCGGA AAGCGTCGGC 
GGCAGTACCG GCGGTGCCTT TAGGCCCACC TCGACGGGCA GTCCCAAGGA GGCGGTGGGC 
GCGGCTGCAG CTGCCGCAGC GGCTGGCTTG AATCCCGGTG GCGGTAGCTA TCCGTCACCG 
AATATATCGG TGGGTCCGCC CATTCACCCG TCGCCGCATT TGTTGCCTTA CCTGTATCCA 
CATGGCCTGT ATCCACCGCC GCATCTGGGC CTGCTGCACA ATCCCGCTGC AGCGGCGGCC 
ATGAGTCCGG CTGGCCTGAA TCCGGGTCTG CTCTTCAATG CCCAACTGGC GCTGGCCGCC 
CAGCATCCGG CTTTGTTTGG CCACGCCTAC GCGGCGGCGG GTCACACACC GGTATCACCA 
TTACAAGGCC TAAAGAGCCA TCGCTTTTCG CCGTACAGTT TGCCCGGCAG TTTGGGCTCC 
GCCTTCGATG CAGTGACTCC TGGATCGAAC GCAAATCGCT CGGGAGATCC TCCAGGCGGC 
GGCGGCGGCG GCCTGGGTGG TGGTGTGGTG GAGAATGGTC CCAGGAGTCT GAGTTCCAGC 
CCTCGTCCCC GACCTGCCTC CCACTCGCCG CCCACTCGAC CGATTTCGAT GTCACCCACA 
ACGCCGCCAT CCCTGATGAA GCAACCACGT GGCGGTGGTG CTGGCGCCGG TGTGGCCCAA 
TCGCAGCACT CGCCTTCGGA ACTTAAGAGC ATGGAGAAGA TGGTCAACGG ACTGGAGGTT 
CAACACAATG GCAGTGCGGC GGCAGCAGCG GCGGCTCTTC AGCTGGCCGA GGAAGCTGCC 
CAGCATCATC ATCACACCCA GGCGCACCAC CAGCAGCAGC AGCATCAGTC GCACCACCAG 
CAACAGCACC ACCAGCAACC GGCACAACCA CATCCGCACC ACCAGACCCA TCTACACTCG 
CACCATGGGG CGACAACGGG CGGTACGGAT CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284861.1
CDS	676..3591
Translation	MRYDVQELLFHQSAEDPFARFANGMAYHPFLQLTQRPTDFSVSSLLTAGSNNNNSGNTNSGNNNSNSNNNTNSNTNNTNNLVAVSPTGGGAQLSPQSNHSSSNTTTTSNTNNSSSNNNNNNSTHNNNNNHTNNNNNNNNNTSQKQGHHLSTTEEPPSPAGTPPPTIVGLPPIPPPNNNSSSSSSNNSASAAAHPSHHPTAAHHSPSTGAAAPPAGATGLPPPTPPHHLQQQQQQQQHPAPPPPPYFPAAALAALAGSPAGPHPGLYPGGGLRFPPHHPGAHPHAHHLGSAYTTAEDVVLASAVAHQLHPAMRPLRALQPEDDGVVDDPKVTLEGKDLWEKFHKLGTEMVITKSGRQMFPQMKFRVSGLDAKAKYILLLDIVAADDYRYKFHNSRWMVAGKADPEMPKRMYIHPDSPTTGEQWMQKVVSFHKLKLTNNISDKHGFTILNSMHKYQPRFHLVRANDILKLPYSTFRTYVFKETEFIAVTAYQNEKITQLKIDNNPFAKGFRDTGAGKREKNCYRQALMSNRGSDSDKLNPTHVSSSRAPLHLGHAGRPPHLHPHAALLDNQQDDDDKLLDVVGPPQSPLLPLSHSLQQMHAHQHSALAAWFNHLAGAGAGASEHAAAAAANASAEDALRRRLQADADVERDGSDSSCSESVGGSTGGAFRPTSTGSPKEAVGAAAAAAAAGLNPGGGSYPSPNISVGPPIHPSPHLLPYLYPHGLYPPPHLGLLHNPAAAAAMSPAGLNPGLLFNAQLALAAQHPALFGHAYAAAGHTPVSPLQGLKSHRFSPYSLPGSLGSAFDAVTPGSNANRSGDPPGGGGGGLGGGVVENGPRSLSSSPRPRPASHSPPTRPISMSPTTPPSLMKQPRGGGAGAGVAQSQHSPSELKSMEKMVNGLEVQHNGSAAAAAAALQLAEEAAQHHHHTQAHHQQQQHQSHHQQQHHQQPAQPHPHHQTHLHSHHGATTGGTDQ

Target ORF information:

RefSeq Version	NM_001297932.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bifid (bi), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297932.1

ORF Insert Sequence:

ATGAGATACG ACGTCCAGGA GCTGCTATTT CATCAGTCTG CTGAGGATCC ATTCGCCAGG 
TTCGCCAATG GGATGGCATA TCATCCATTT CTGCAGCTAA CGCAACGACC CACTGACTTC 
AGCGTTTCCT CGCTGTTGAC GGCGGGTAGC AACAATAACA ACAGCGGCAA CACCAACAGC 
GGAAACAACA ACTCCAATTC CAACAACAAC ACCAATTCAA ACACCAATAA CACCAACAAT 
CTCGTGGCCG TTTCACCAAC GGGTGGTGGT GCGCAGTTAT CGCCGCAAAG CAACCACAGC 
AGCAGCAACA CCACCACCAC CAGCAACACC AACAACTCCA GTTCCAATAA CAACAATAAT 
AACAGTACCC ATAATAATAA CAACAACCAC ACGAATAACA ACAACAATAA TAATAATAAC 
ACAAGTCAAA AGCAAGGACA CCACTTGAGC ACCACCGAGG AACCGCCATC GCCCGCTGGC 
ACACCACCGC CCACAATTGT TGGTCTACCG CCAATACCAC CGCCCAACAA CAACAGCAGT 
AGCAGCAGCA GCAACAACTC AGCTTCAGCC GCCGCCCATC CGTCGCACCA CCCAACCGCC 
GCACATCACT CACCCAGTAC GGGTGCCGCC GCACCGCCCG CCGGCGCCAC AGGCTTGCCG 
CCACCCACAC CGCCGCACCA CCTGCAACAA CAACAGCAGC AACAACAGCA CCCGGCCCCA 
CCACCACCAC CGTACTTTCC AGCTGCGGCA CTGGCCGCTC TAGCTGGAAG TCCGGCCGGA 
CCGCATCCGG GTCTGTATCC CGGCGGTGGA TTGCGCTTCC CGCCGCACCA CCCAGGTGCC 
CATCCGCACG CCCATCATCT GGGCAGCGCC TATACTACCG CCGAGGACGT CGTCCTTGCC 
TCGGCCGTCG CCCATCAGCT GCATCCGGCG ATGCGACCGC TGCGGGCGCT TCAGCCCGAG 
GACGATGGCG TCGTCGATGA TCCCAAGGTC ACGCTGGAGG GCAAAGACCT GTGGGAGAAG 
TTCCACAAAC TGGGCACGGA AATGGTCATC ACCAAGAGCG GCAGACAAAT GTTTCCGCAA 
ATGAAATTTC GTGTTTCGGG ACTGGATGCC AAGGCTAAAT ACATCTTGCT ACTGGACATT 
GTGGCGGCGG ACGATTATCG TTATAAATTT CATAATAGTC GCTGGATGGT GGCTGGCAAA 
GCGGATCCCG AGATGCCAAA ACGCATGTAT ATCCATCCAG ATTCGCCCAC AACGGGTGAG 
CAATGGATGC AGAAAGTTGT TTCATTTCAC AAATTAAAAT TGACCAACAA TATTAGTGAT 
AAACATGGAT TTACGATCCT GAACTCGATG CACAAGTACC AGCCGCGTTT CCACCTGGTG 
CGAGCCAATG ACATCCTGAA GCTGCCGTAC TCCACGTTTC GCACGTACGT CTTCAAGGAG 
ACCGAGTTCA TCGCCGTCAC AGCATATCAA AATGAGAAGA TAACTCAATT GAAAATCGAT 
AACAATCCGT TTGCGAAGGG CTTTCGTGAT ACTGGTGCCG GCAAGCGGGA AAAGAATTGT 
TACAGGCAGG CACTGATGTC GAACCGAGGG TCCGATTCGG ACAAGTTGAA TCCGACGCAT 
GTGAGCAGCT CGCGGGCACC GCTCCACCTG GGCCACGCCG GCCGTCCGCC TCATCTGCAT 
CCCCATGCCG CCCTGCTTGA TAATCAGCAG GACGACGACG ACAAGCTCCT GGACGTGGTG 
GGTCCGCCAC AGAGTCCGCT ACTCCCGCTC AGCCACTCGC TGCAGCAGAT GCACGCCCAC 
CAGCACTCCG CTCTGGCTGC CTGGTTTAAT CACTTGGCTG GCGCTGGAGC CGGAGCCTCC 
GAACATGCAG CTGCGGCGGC GGCCAATGCC AGTGCGGAGG ATGCACTGCG TCGTCGCTTG 
CAGGCGGACG CGGATGTGGA GCGGGATGGC AGCGATTCGA GTTGCTCGGA AAGCGTCGGC 
GGCAGTACCG GCGGTGCCTT TAGGCCCACC TCGACGGGCA GTCCCAAGGA GGCGGTGGGC 
GCGGCTGCAG CTGCCGCAGC GGCTGGCTTG AATCCCGGTG GCGGTAGCTA TCCGTCACCG 
AATATATCGG TGGGTCCGCC CATTCACCCG TCGCCGCATT TGTTGCCTTA CCTGTATCCA 
CATGGCCTGT ATCCACCGCC GCATCTGGGC CTGCTGCACA ATCCCGCTGC AGCGGCGGCC 
ATGAGTCCGG CTGGCCTGAA TCCGGGTCTG CTCTTCAATG CCCAACTGGC GCTGGCCGCC 
CAGCATCCGG CTTTGTTTGG CCACGCCTAC GCGGCGGCGG GTCACACACC GGTATCACCA 
TTACAAGGCC TAAAGAGCCA TCGCTTTTCG CCGTACAGTT TGCCCGGCAG TTTGGGCTCC 
GCCTTCGATG CAGTGACTCC TGGATCGAAC GCAAATCGCT CGGGAGATCC TCCAGGCGGC 
GGCGGCGGCG GCCTGGGTGG TGGTGTGGTG GAGAATGGTC CCAGGAGTCT GAGTTCCAGC 
CCTCGTCCCC GACCTGCCTC CCACTCGCCG CCCACTCGAC CGATTTCGAT GTCACCCACA 
ACGCCGCCAT CCCTGATGAA GCAACCACGT GGCGGTGGTG CTGGCGCCGG TGTGGCCCAA 
TCGCAGCACT CGCCTTCGGA ACTTAAGAGC ATGGAGAAGA TGGTCAACGG ACTGGAGGTT 
CAACACAATG GCAGTGCGGC GGCAGCAGCG GCGGCTCTTC AGCTGGCCGA GGAAGCTGCC 
CAGCATCATC ATCACACCCA GGCGCACCAC CAGCAGCAGC AGCATCAGTC GCACCACCAG 
CAACAGCACC ACCAGCAACC GGCACAACCA CATCCGCACC ACCAGACCCA TCTACACTCG 
CACCATGGGG CGACAACGGG CGGTACGGAT CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bi cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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