tyf cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following tyf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tyf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa00756 NM_131924.3
Latest version!
Drosophila melanogaster twenty-four (tyf), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa00756 NM_001297924.2
Latest version!
Drosophila melanogaster twenty-four (tyf), transcript variant N, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa00756 NM_001258590.2
Latest version!
Drosophila melanogaster twenty-four (tyf), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa00756 NM_001169186.2
Latest version!
Drosophila melanogaster twenty-four (tyf), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa00756 NM_001169187.2
Latest version!
Drosophila melanogaster twenty-four (tyf), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa03750 NM_001258588.2
Latest version!
Drosophila melanogaster twenty-four (tyf), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa03750 NM_001169189.3
Latest version!
Drosophila melanogaster twenty-four (tyf), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa03750 NM_001169188.3
Latest version!
Drosophila melanogaster twenty-four (tyf), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa03750 NM_001258592.2
Latest version!
Drosophila melanogaster twenty-four (tyf), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa03750 NM_001258589.2
Latest version!
Drosophila melanogaster twenty-four (tyf), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa03750 NM_001258591.2
Latest version!
Drosophila melanogaster twenty-four (tyf), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa00756
    Clone ID Related Accession (Same CDS sequence) NM_001169187.2 , NM_131924.3 , NM_001169186.2 , NM_001258590.2 , NM_001297924.2
    Accession Version NM_001169187.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twenty-four, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_001169187.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGAACAATG AACAAACGCC GTCATCATCG GACACACCGG CGGATCGTGT GGCATCTGGT 
    GGAGCAGCTG CCAAACTGCC AACGATCATA CCGGACACGC CCATCGTCTC GATTGCCGCT
    GGCACGATGA GCGAGAGCAC CACGACGACC ACCGTCACCA AAACAACGCC GACGCCGTTC
    CCACAGGAGC AGCTTATGAC CATGATGTCA GACCATGTGG CGAAGGCAGA CGCGCAGCTT
    GACGAGGCCT GTAGGAACCA GGGGGCCAAA GACGAGGACA GCCAAGGAGC GGCACCAGTG
    GCTGCCAACC ATGTAGCCAC CAGCGGCAGT AGCAGCAGAG GCAATGACCC CAAGAAGTCA
    TCATCCCCTA GTAATAGCCA TAGCTCTGGT AACCACGCAG CCACGGCAGA CGCTGCCAAA
    GGATCCGCAG CAGAAGCAGC AGCAGGAGAT ACGTCCTTCT CGCAGGTCTT TGCCATGCTA
    AAGGATTCGC CCAGCGCTGA ACTGTCGCGA CCAAACCGCA GGGTACTCCA GTACGGAGCC
    ACTGCCAAGG GCAAGACCCA GGACGATCAG AATGTCGGCA AGCGACAGAA GAAGAAAAAG
    ACAAAATACG ATACCTGGGA AGATAGCGAT TTTAATGATC TGGACGACGC ATCATTGAAG
    AAACTTCTCG AAGAGGCCTA CTGGTATCGA AATCCTGGCG ACAGGAAGAA CAAGAGCGAG
    CGATTTCTGC AAATGCTCAA AAAGGCTGAG TACGACGAAG AGATCTCTTA TCGTGCCATC
    AAATCCTGCC TCACAATCAA CCCATCCGCA CTAACGTCCA GTGCAGCAAC AGGCTCGAGC
    GGCGGGAGCA GCAGCAGTGC AGGGCACAAC AGCAGCTATA ATTCCACCAA TCACCGGTCA
    ACGGATCCCG GCCAGCGGCA CAAGCAGGGC GGCTCCCTGC AGGATCTCGT TGAGGCGGCG
    CACCGCAGCG AACTGGCCAC AACCTCGGCG GCGGCGGCAG CAGCAGCGAC AGCGGCAGCA
    ACCCAACGTT TCAACTGTGA TTACCAGCTG AGTGGTCGCT CCAATCGCCG GCAGCAGCAG
    CAGCAACAGC AGCACAACCA AAACGCCACC TCCTCCGCAT CCGCATCCAA AAAGATCAAG
    AACTTAAACG TCTCGGGCCG GCAGCGCGAA GGAGGCAGCC TGCCCAGCAG TGTTAACTTT
    GAGCCGGCAT CGTCTGCCAT GGAAGCCAAG CAGAATAAGC AACAGGCTCA GCCTTCGACA
    GAAGCTGCAG GTGGCTCGTT TTCGGGGTCG GTCGGTAACA AGCACCACCA CCGCAGCAGC
    ACCGGCAGTT GCAGCAGCCT GATAACTCAA TCGCGCGATA CGGAGGCAAA CAATCAGTTC
    GACTTGGACA TGGACGATGC GACGGGTGTT GGTGCCAGCG CCGTTGTCGG TGCCAGCGGC
    GACCAAGACG ACAACATGTT GGAGCAATTG GATCCTCAGA ACCCCTCGAT GAAGTTTCTG
    ATTGACGCCC TAAACGACAC GCCATTGGAG GCCAGGTACA CCGGTGCCGG CAAAAAGTTC
    CTAATCGACG ACCGGTTGCA TTTTTCCGTC CTGGAGCTGT CTGCCAGGAG GAAGCTGCAG
    CAGCAGGCAC AATACCTGGC CGCCCTCACC GGACAATTTG GTCTCGGCCT GGAGGAGGAG
    AAGCGCAAGG TAGAGGCCGG CTACGTGTCG CTCAACGACA ACTACACGGC GTGCTCCTCA
    GTTTACGGCG GCGGTGCAGG TTCGCCAGCT GCCGTGGAGG GCAGCAAGCC CTCTACCTCC
    TCCTCGGCCG TGTCTGGCGG TAGCAACTGC GGTGCCTCCT CTGACGCACT GTCTAGCGAT
    CTGCGACCTG CGAAGATTGG ACGCATGGTC CCCGCCGCAG CGGCAGTGGC AGCTGCGCCA
    ATGGGTGGCA AGACGACTAC TCGACAGCTA GACGAGAATG GAAATGCATT GGGCGATGGT
    TTCGGGGGCA GTGCAGCCAG TGGCTCCAGT GGCGCCAGTG GCGCCAGTGC TGCCGCCAGC
    AGTAGCAACG GCAACGGTAA CGCAAACCAG TTCACAGCTC TAATAACCCC CACGGTGGGA
    TCCAGTGTAC CCACCTCTAC TGTCGCTCAC CGTCAGAATA GTGTCTCCAC GCTGCTCTCC
    ACGGGCACCA ACACTACAAC AACGGCCATG GCAGCGGCGG CGGTGGCTGC ATCCTACAGC
    CAGTTGCAAC AGGCACCGGT CGGAGGCGCG GCTGGAACGG TTTCTGGGAT CGGCATTGGC
    ATGCAGCAGC CCACCAAGCA GAAGACGAAG AAGAAGTCGC AGCAGGAGCG CAACACCACC
    ACCGTCGATG TGGAGTCGGT TGCCGGCTAT AGGGGCAATG ATCCTGTTGA GCAGCTCGTC
    AAGTACATTG AAAACGATGT CAACGGTGGC GGAAACAGTG CCGCTGGACA GCGCAAGAAG
    GAGCGCAAGA AGCAAAACAA GCTCAAGAAG AGCAACTCGT TGGAGGAGCT GCGCACCTGC
    TCAAAAATGG AGGTGGACGA TTTGAAGCGC CAGTCGGCGA CCACGGAGAT GATGCGACAT
    AAGAAGGGCA CGAACAATGC ATCCTCGGGC GGATCGTCGT CGACGGCCTC TGGTTCCTCA
    AACAGTGGCA AGCACAACTC GGCATCCGTG GCGGATATTA ACAAAAACTG CAACAAGGAG
    CAGCAACAGC AATCGACGCC GACGGTGCAA GTGCGCAACA GCAACAATCC AGGAACGCAG
    CAGCGGAAGG GCGAGCGTCG GTCCTGGGGC ACCGAGGAGT TGCAGTACCT GGGTGACCAC
    CAGGAAATAT CCGCTGCCTG GTCGGAGCTG GAGACTGTGG GCCAGAAGGA GCTGCCGCTG
    GCGCTGCCCG CCTTGGCGCG CATGTCCGAA CTGGATGCCC TGAATACCGT CTTGTCGGAG
    ACCGCTGAGT TCCATGTGGT TACCAAGAAG AAGAAACCAA AGAAGCAGCG TGCTGTTACC
    ATGGACGATG CGGCGGTGGC TGCAGCTGCC GTCACGGGCG GCAACTTACA GCGCATGCAG
    CAGATCACCA AGTCCGCGTC CTCCAACATC ATGTCCCAGC GGATGCACTA CTATACTCCG
    TACAACAGCA ACAATGGTGG AGGCAGTGTC AACTATAAGC AACAGCAGCA ACAGAGCCAA
    CAGTACCAGG AGCACTACCA GGCGCCACAG GGGCAGCACC ACCATCACCA CCATCATCAT
    CACCACCATC ATCACCATCA CAGCGGCTCG GCGGTTTCGA ATCAGGTGGA TAGCTCACGG
    CGCAAGTCCA CGTCGTCTAT GCCCCCATCG GAGAAATCTG ACTCCAGTGA TCTCGACTCG
    GTCCACTCCC TGCCCATCCA GACGGGGAAG AAGAAGAGCC TAGGCGGTGG CAACAACAAA
    CAACGGGGGG CGCCGTCAGC CAGTCAACGC CAGGCCAAGC AAAACAACAA TGCTGCAGCC
    CCCATTTCGT ATGCGGATAT CGCCCGGAAC AAGCAGGAGG CTCTAAACAA TGCCACGGCC
    AGCGACACCG AGTTGGAGCT GGGCGACAAG ATGCAGATCA AGAGTGGCGG CAAATCCAAG
    TCGCGACCCG ATTTCCCGGA GCTGCCAGGT GCTGTTACGG CAGTGGCTGC CAGTGGAGGT
    GCAGCCAATC AGGCGACGGT TGTCAGCAGC CAAAATACGC CACCATCCTC GTCCAGTTCC
    ATCAGTTATT CGCAGAGTCT CAATGCAACG CCGCCCAGCA GCAGCAGCGA TGCGGAGTCC
    ACCAACTCGC CGGAGCTGCT GGCGCAGGTG CAGATACCGG CTTACAGCAT GATCACGCCA
    ACTCTAACAG CCTCCATGAT CAGCAGTGTC AGCAGTTCTG TCGCCAGCAC CTCTTCCACA
    TCCTGCTCAC CGCCAGCTCT GCAGAAATCC AAGAGCGTGG AGCACGACAC CAGCTACAGC
    TGCAATAGTA GCAACTTGGA TCAACAGTAT CCGGCACTAG AAAAGACCGT CAAGCGGCAT
    AGCACCAACA ACGTGTCTGT GGCCGTCGCA GCCTCCACCT CGGCATCGGC GTCCTCCTCG
    TTGTACAACT TTGCAGCGGC AGCCAAGCAG CAGCTGACAG AGAACACCCA TTCGGCCGTA
    TCGCCGCCGG CAGCTGTAAC AACTACCTCA ACCGCAGTCG CAGCCTCAGC TCCAGCTCCA
    ACTACAGTTA CAGTTCCAGC TGCAGTTACA GTTCCTTGTT ATTCCTCATC CTCCACACAC
    GGCTCGACTC AAACTTCAAC TTCTACTGCA TCGTCTGCTA TAGCAGCTTT ACCCTCGGGC
    AAGGCCAAGG CCAAGCCAAA GGAGCTATCT CCCAGCAGCA GTTGCAGCAA GAAGCCAACG
    AAGCCTAGTC AAGATGTGCC GTCAATCAGT CTGTCCATTT CCACCACGCC AAGGGCGACG
    ACCAGCACCA GCACTACTAC TACACAAAAG ACCACGGCTG CCACGCAGAC CGAGGGAGCC
    AAAAAGCTAA TCGGCGGGAT CCACAAGCTG CCCAGTAGCA GTTGTATTGC GAAGGGAGCC
    GGAGCCGTTT TGGAGGCCAC CGCCGGCAGG CGTGCTGTTA TCATACTCAA CGATGATCGC
    GAAGCAGGCA GGAGCAACAA TGAGTTCATA TTCGGTGATT TCAACGAGGA TGAGCTAAAA
    CTCTTCGACG ACAACTTGGA TGATGAGGAG GAGGGCCAGC ACAAGCAGTC TTCAAACAAG
    AAACAGCAAA CTGAAGGGGA ATCTGATGTG ACCCAAGATG ACACCGAAGA CGTGGACCAA
    GATCTGGACA AGGAACTGGA ATGCCTGGGA CGACGACCAG AGAAGAAGCA AGAGCGACAG
    CAGGATATCA GCGCCAGCAG CGATCAGCAT CTGAATGATT CTGGTGCGGC TTCGGATGCA
    GTCAACAGCT CAGCCAGCCT TGACATGCTC TCAATTTCTG CAGAGGCGGC ACAATCGTCA
    CCTAATGCGG GGGCAACCGC TACCTCATCC AGCGTCGCCA GTCCCATTAA CTCGTCCACG
    CCCTCGGGTG CTTCGTCCGC ATCGGCGAGC ACGTCCACCT CATCGTCCTC GGTCTCGATT
    TCATCATCGT CGGGCGGCAA CGGTGCCGCT TGTCTTGCAT CCGTTTCCGG TATGCTTGGT
    GGAGTGGCCA ATCAGTCCGG GGACAGCGGC ATCTATGCTG CCGCCAACAC GTCTATTAAG
    GAGCACGTTA ACACTTTCCT AAGCAAGGCC AGCAGCAGCG AGGAGACGCT GCCGAGTCCC
    AACTCCATAT CGATGCAACA GCTGGAAACA TGCAACGACA TCGAGGCGGC CATCATAGCC
    GCAGCCCGTG CTGCCGCTGC TGCCCGGAGC ACCAGCTGCA GTCGCAGCAA CTCTCAGGAG
    CAGGATGCCT TGCAGCCGCA GGTCAAGACC AAGGCGACTC CCAATCCGGA GGCGAAGATG
    GTTCTGCCTG TGTTTGTGAC CTACAACAAC AACAACAACG ACGACGATGA CGACGAGGAG
    GACGACGAGA GCCTGCAAGA ACTGAGCTTC ATGGCCGACC TTAAGGCGGA TGCAGCCACG
    GAGGATATTG CAATGCAGCC GCCACCGCCT TCATGTCCGG CGATGATGAC CAACACGGAA
    CTAATCGTCG ACTACATCGC CAAAACCTGG AATGCCATTG CCAACAGTAA GTACGTAACG
    TACTACAACG AGCAAGAGCA GGAGCAGGAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162658.2
    CDS755..6490
    Translation

    Target ORF information:

    RefSeq Version NM_001169187.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twenty-four (tyf), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169187.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGAACAATG AACAAACGCC GTCATCATCG GACACACCGG CGGATCGTGT GGCATCTGGT 
    GGAGCAGCTG CCAAACTGCC AACGATCATA CCGGACACGC CCATCGTCTC GATTGCCGCT
    GGCACGATGA GCGAGAGCAC CACGACGACC ACCGTCACCA AAACAACGCC GACGCCGTTC
    CCACAGGAGC AGCTTATGAC CATGATGTCA GACCATGTGG CGAAGGCAGA CGCGCAGCTT
    GACGAGGCCT GTAGGAACCA GGGGGCCAAA GACGAGGACA GCCAAGGAGC GGCACCAGTG
    GCTGCCAACC ATGTAGCCAC CAGCGGCAGT AGCAGCAGAG GCAATGACCC CAAGAAGTCA
    TCATCCCCTA GTAATAGCCA TAGCTCTGGT AACCACGCAG CCACGGCAGA CGCTGCCAAA
    GGATCCGCAG CAGAAGCAGC AGCAGGAGAT ACGTCCTTCT CGCAGGTCTT TGCCATGCTA
    AAGGATTCGC CCAGCGCTGA ACTGTCGCGA CCAAACCGCA GGGTACTCCA GTACGGAGCC
    ACTGCCAAGG GCAAGACCCA GGACGATCAG AATGTCGGCA AGCGACAGAA GAAGAAAAAG
    ACAAAATACG ATACCTGGGA AGATAGCGAT TTTAATGATC TGGACGACGC ATCATTGAAG
    AAACTTCTCG AAGAGGCCTA CTGGTATCGA AATCCTGGCG ACAGGAAGAA CAAGAGCGAG
    CGATTTCTGC AAATGCTCAA AAAGGCTGAG TACGACGAAG AGATCTCTTA TCGTGCCATC
    AAATCCTGCC TCACAATCAA CCCATCCGCA CTAACGTCCA GTGCAGCAAC AGGCTCGAGC
    GGCGGGAGCA GCAGCAGTGC AGGGCACAAC AGCAGCTATA ATTCCACCAA TCACCGGTCA
    ACGGATCCCG GCCAGCGGCA CAAGCAGGGC GGCTCCCTGC AGGATCTCGT TGAGGCGGCG
    CACCGCAGCG AACTGGCCAC AACCTCGGCG GCGGCGGCAG CAGCAGCGAC AGCGGCAGCA
    ACCCAACGTT TCAACTGTGA TTACCAGCTG AGTGGTCGCT CCAATCGCCG GCAGCAGCAG
    CAGCAACAGC AGCACAACCA AAACGCCACC TCCTCCGCAT CCGCATCCAA AAAGATCAAG
    AACTTAAACG TCTCGGGCCG GCAGCGCGAA GGAGGCAGCC TGCCCAGCAG TGTTAACTTT
    GAGCCGGCAT CGTCTGCCAT GGAAGCCAAG CAGAATAAGC AACAGGCTCA GCCTTCGACA
    GAAGCTGCAG GTGGCTCGTT TTCGGGGTCG GTCGGTAACA AGCACCACCA CCGCAGCAGC
    ACCGGCAGTT GCAGCAGCCT GATAACTCAA TCGCGCGATA CGGAGGCAAA CAATCAGTTC
    GACTTGGACA TGGACGATGC GACGGGTGTT GGTGCCAGCG CCGTTGTCGG TGCCAGCGGC
    GACCAAGACG ACAACATGTT GGAGCAATTG GATCCTCAGA ACCCCTCGAT GAAGTTTCTG
    ATTGACGCCC TAAACGACAC GCCATTGGAG GCCAGGTACA CCGGTGCCGG CAAAAAGTTC
    CTAATCGACG ACCGGTTGCA TTTTTCCGTC CTGGAGCTGT CTGCCAGGAG GAAGCTGCAG
    CAGCAGGCAC AATACCTGGC CGCCCTCACC GGACAATTTG GTCTCGGCCT GGAGGAGGAG
    AAGCGCAAGG TAGAGGCCGG CTACGTGTCG CTCAACGACA ACTACACGGC GTGCTCCTCA
    GTTTACGGCG GCGGTGCAGG TTCGCCAGCT GCCGTGGAGG GCAGCAAGCC CTCTACCTCC
    TCCTCGGCCG TGTCTGGCGG TAGCAACTGC GGTGCCTCCT CTGACGCACT GTCTAGCGAT
    CTGCGACCTG CGAAGATTGG ACGCATGGTC CCCGCCGCAG CGGCAGTGGC AGCTGCGCCA
    ATGGGTGGCA AGACGACTAC TCGACAGCTA GACGAGAATG GAAATGCATT GGGCGATGGT
    TTCGGGGGCA GTGCAGCCAG TGGCTCCAGT GGCGCCAGTG GCGCCAGTGC TGCCGCCAGC
    AGTAGCAACG GCAACGGTAA CGCAAACCAG TTCACAGCTC TAATAACCCC CACGGTGGGA
    TCCAGTGTAC CCACCTCTAC TGTCGCTCAC CGTCAGAATA GTGTCTCCAC GCTGCTCTCC
    ACGGGCACCA ACACTACAAC AACGGCCATG GCAGCGGCGG CGGTGGCTGC ATCCTACAGC
    CAGTTGCAAC AGGCACCGGT CGGAGGCGCG GCTGGAACGG TTTCTGGGAT CGGCATTGGC
    ATGCAGCAGC CCACCAAGCA GAAGACGAAG AAGAAGTCGC AGCAGGAGCG CAACACCACC
    ACCGTCGATG TGGAGTCGGT TGCCGGCTAT AGGGGCAATG ATCCTGTTGA GCAGCTCGTC
    AAGTACATTG AAAACGATGT CAACGGTGGC GGAAACAGTG CCGCTGGACA GCGCAAGAAG
    GAGCGCAAGA AGCAAAACAA GCTCAAGAAG AGCAACTCGT TGGAGGAGCT GCGCACCTGC
    TCAAAAATGG AGGTGGACGA TTTGAAGCGC CAGTCGGCGA CCACGGAGAT GATGCGACAT
    AAGAAGGGCA CGAACAATGC ATCCTCGGGC GGATCGTCGT CGACGGCCTC TGGTTCCTCA
    AACAGTGGCA AGCACAACTC GGCATCCGTG GCGGATATTA ACAAAAACTG CAACAAGGAG
    CAGCAACAGC AATCGACGCC GACGGTGCAA GTGCGCAACA GCAACAATCC AGGAACGCAG
    CAGCGGAAGG GCGAGCGTCG GTCCTGGGGC ACCGAGGAGT TGCAGTACCT GGGTGACCAC
    CAGGAAATAT CCGCTGCCTG GTCGGAGCTG GAGACTGTGG GCCAGAAGGA GCTGCCGCTG
    GCGCTGCCCG CCTTGGCGCG CATGTCCGAA CTGGATGCCC TGAATACCGT CTTGTCGGAG
    ACCGCTGAGT TCCATGTGGT TACCAAGAAG AAGAAACCAA AGAAGCAGCG TGCTGTTACC
    ATGGACGATG CGGCGGTGGC TGCAGCTGCC GTCACGGGCG GCAACTTACA GCGCATGCAG
    CAGATCACCA AGTCCGCGTC CTCCAACATC ATGTCCCAGC GGATGCACTA CTATACTCCG
    TACAACAGCA ACAATGGTGG AGGCAGTGTC AACTATAAGC AACAGCAGCA ACAGAGCCAA
    CAGTACCAGG AGCACTACCA GGCGCCACAG GGGCAGCACC ACCATCACCA CCATCATCAT
    CACCACCATC ATCACCATCA CAGCGGCTCG GCGGTTTCGA ATCAGGTGGA TAGCTCACGG
    CGCAAGTCCA CGTCGTCTAT GCCCCCATCG GAGAAATCTG ACTCCAGTGA TCTCGACTCG
    GTCCACTCCC TGCCCATCCA GACGGGGAAG AAGAAGAGCC TAGGCGGTGG CAACAACAAA
    CAACGGGGGG CGCCGTCAGC CAGTCAACGC CAGGCCAAGC AAAACAACAA TGCTGCAGCC
    CCCATTTCGT ATGCGGATAT CGCCCGGAAC AAGCAGGAGG CTCTAAACAA TGCCACGGCC
    AGCGACACCG AGTTGGAGCT GGGCGACAAG ATGCAGATCA AGAGTGGCGG CAAATCCAAG
    TCGCGACCCG ATTTCCCGGA GCTGCCAGGT GCTGTTACGG CAGTGGCTGC CAGTGGAGGT
    GCAGCCAATC AGGCGACGGT TGTCAGCAGC CAAAATACGC CACCATCCTC GTCCAGTTCC
    ATCAGTTATT CGCAGAGTCT CAATGCAACG CCGCCCAGCA GCAGCAGCGA TGCGGAGTCC
    ACCAACTCGC CGGAGCTGCT GGCGCAGGTG CAGATACCGG CTTACAGCAT GATCACGCCA
    ACTCTAACAG CCTCCATGAT CAGCAGTGTC AGCAGTTCTG TCGCCAGCAC CTCTTCCACA
    TCCTGCTCAC CGCCAGCTCT GCAGAAATCC AAGAGCGTGG AGCACGACAC CAGCTACAGC
    TGCAATAGTA GCAACTTGGA TCAACAGTAT CCGGCACTAG AAAAGACCGT CAAGCGGCAT
    AGCACCAACA ACGTGTCTGT GGCCGTCGCA GCCTCCACCT CGGCATCGGC GTCCTCCTCG
    TTGTACAACT TTGCAGCGGC AGCCAAGCAG CAGCTGACAG AGAACACCCA TTCGGCCGTA
    TCGCCGCCGG CAGCTGTAAC AACTACCTCA ACCGCAGTCG CAGCCTCAGC TCCAGCTCCA
    ACTACAGTTA CAGTTCCAGC TGCAGTTACA GTTCCTTGTT ATTCCTCATC CTCCACACAC
    GGCTCGACTC AAACTTCAAC TTCTACTGCA TCGTCTGCTA TAGCAGCTTT ACCCTCGGGC
    AAGGCCAAGG CCAAGCCAAA GGAGCTATCT CCCAGCAGCA GTTGCAGCAA GAAGCCAACG
    AAGCCTAGTC AAGATGTGCC GTCAATCAGT CTGTCCATTT CCACCACGCC AAGGGCGACG
    ACCAGCACCA GCACTACTAC TACACAAAAG ACCACGGCTG CCACGCAGAC CGAGGGAGCC
    AAAAAGCTAA TCGGCGGGAT CCACAAGCTG CCCAGTAGCA GTTGTATTGC GAAGGGAGCC
    GGAGCCGTTT TGGAGGCCAC CGCCGGCAGG CGTGCTGTTA TCATACTCAA CGATGATCGC
    GAAGCAGGCA GGAGCAACAA TGAGTTCATA TTCGGTGATT TCAACGAGGA TGAGCTAAAA
    CTCTTCGACG ACAACTTGGA TGATGAGGAG GAGGGCCAGC ACAAGCAGTC TTCAAACAAG
    AAACAGCAAA CTGAAGGGGA ATCTGATGTG ACCCAAGATG ACACCGAAGA CGTGGACCAA
    GATCTGGACA AGGAACTGGA ATGCCTGGGA CGACGACCAG AGAAGAAGCA AGAGCGACAG
    CAGGATATCA GCGCCAGCAG CGATCAGCAT CTGAATGATT CTGGTGCGGC TTCGGATGCA
    GTCAACAGCT CAGCCAGCCT TGACATGCTC TCAATTTCTG CAGAGGCGGC ACAATCGTCA
    CCTAATGCGG GGGCAACCGC TACCTCATCC AGCGTCGCCA GTCCCATTAA CTCGTCCACG
    CCCTCGGGTG CTTCGTCCGC ATCGGCGAGC ACGTCCACCT CATCGTCCTC GGTCTCGATT
    TCATCATCGT CGGGCGGCAA CGGTGCCGCT TGTCTTGCAT CCGTTTCCGG TATGCTTGGT
    GGAGTGGCCA ATCAGTCCGG GGACAGCGGC ATCTATGCTG CCGCCAACAC GTCTATTAAG
    GAGCACGTTA ACACTTTCCT AAGCAAGGCC AGCAGCAGCG AGGAGACGCT GCCGAGTCCC
    AACTCCATAT CGATGCAACA GCTGGAAACA TGCAACGACA TCGAGGCGGC CATCATAGCC
    GCAGCCCGTG CTGCCGCTGC TGCCCGGAGC ACCAGCTGCA GTCGCAGCAA CTCTCAGGAG
    CAGGATGCCT TGCAGCCGCA GGTCAAGACC AAGGCGACTC CCAATCCGGA GGCGAAGATG
    GTTCTGCCTG TGTTTGTGAC CTACAACAAC AACAACAACG ACGACGATGA CGACGAGGAG
    GACGACGAGA GCCTGCAAGA ACTGAGCTTC ATGGCCGACC TTAAGGCGGA TGCAGCCACG
    GAGGATATTG CAATGCAGCC GCCACCGCCT TCATGTCCGG CGATGATGAC CAACACGGAA
    CTAATCGTCG ACTACATCGC CAAAACCTGG AATGCCATTG CCAACAGTAA GTACGTAACG
    TACTACAACG AGCAAGAGCA GGAGCAGGAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00756
    Clone ID Related Accession (Same CDS sequence) NM_001169187.2 , NM_131924.3 , NM_001169186.2 , NM_001258590.2 , NM_001297924.2
    Accession Version NM_131924.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twenty-four, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_131924.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGAACAATG AACAAACGCC GTCATCATCG GACACACCGG CGGATCGTGT GGCATCTGGT 
    GGAGCAGCTG CCAAACTGCC AACGATCATA CCGGACACGC CCATCGTCTC GATTGCCGCT
    GGCACGATGA GCGAGAGCAC CACGACGACC ACCGTCACCA AAACAACGCC GACGCCGTTC
    CCACAGGAGC AGCTTATGAC CATGATGTCA GACCATGTGG CGAAGGCAGA CGCGCAGCTT
    GACGAGGCCT GTAGGAACCA GGGGGCCAAA GACGAGGACA GCCAAGGAGC GGCACCAGTG
    GCTGCCAACC ATGTAGCCAC CAGCGGCAGT AGCAGCAGAG GCAATGACCC CAAGAAGTCA
    TCATCCCCTA GTAATAGCCA TAGCTCTGGT AACCACGCAG CCACGGCAGA CGCTGCCAAA
    GGATCCGCAG CAGAAGCAGC AGCAGGAGAT ACGTCCTTCT CGCAGGTCTT TGCCATGCTA
    AAGGATTCGC CCAGCGCTGA ACTGTCGCGA CCAAACCGCA GGGTACTCCA GTACGGAGCC
    ACTGCCAAGG GCAAGACCCA GGACGATCAG AATGTCGGCA AGCGACAGAA GAAGAAAAAG
    ACAAAATACG ATACCTGGGA AGATAGCGAT TTTAATGATC TGGACGACGC ATCATTGAAG
    AAACTTCTCG AAGAGGCCTA CTGGTATCGA AATCCTGGCG ACAGGAAGAA CAAGAGCGAG
    CGATTTCTGC AAATGCTCAA AAAGGCTGAG TACGACGAAG AGATCTCTTA TCGTGCCATC
    AAATCCTGCC TCACAATCAA CCCATCCGCA CTAACGTCCA GTGCAGCAAC AGGCTCGAGC
    GGCGGGAGCA GCAGCAGTGC AGGGCACAAC AGCAGCTATA ATTCCACCAA TCACCGGTCA
    ACGGATCCCG GCCAGCGGCA CAAGCAGGGC GGCTCCCTGC AGGATCTCGT TGAGGCGGCG
    CACCGCAGCG AACTGGCCAC AACCTCGGCG GCGGCGGCAG CAGCAGCGAC AGCGGCAGCA
    ACCCAACGTT TCAACTGTGA TTACCAGCTG AGTGGTCGCT CCAATCGCCG GCAGCAGCAG
    CAGCAACAGC AGCACAACCA AAACGCCACC TCCTCCGCAT CCGCATCCAA AAAGATCAAG
    AACTTAAACG TCTCGGGCCG GCAGCGCGAA GGAGGCAGCC TGCCCAGCAG TGTTAACTTT
    GAGCCGGCAT CGTCTGCCAT GGAAGCCAAG CAGAATAAGC AACAGGCTCA GCCTTCGACA
    GAAGCTGCAG GTGGCTCGTT TTCGGGGTCG GTCGGTAACA AGCACCACCA CCGCAGCAGC
    ACCGGCAGTT GCAGCAGCCT GATAACTCAA TCGCGCGATA CGGAGGCAAA CAATCAGTTC
    GACTTGGACA TGGACGATGC GACGGGTGTT GGTGCCAGCG CCGTTGTCGG TGCCAGCGGC
    GACCAAGACG ACAACATGTT GGAGCAATTG GATCCTCAGA ACCCCTCGAT GAAGTTTCTG
    ATTGACGCCC TAAACGACAC GCCATTGGAG GCCAGGTACA CCGGTGCCGG CAAAAAGTTC
    CTAATCGACG ACCGGTTGCA TTTTTCCGTC CTGGAGCTGT CTGCCAGGAG GAAGCTGCAG
    CAGCAGGCAC AATACCTGGC CGCCCTCACC GGACAATTTG GTCTCGGCCT GGAGGAGGAG
    AAGCGCAAGG TAGAGGCCGG CTACGTGTCG CTCAACGACA ACTACACGGC GTGCTCCTCA
    GTTTACGGCG GCGGTGCAGG TTCGCCAGCT GCCGTGGAGG GCAGCAAGCC CTCTACCTCC
    TCCTCGGCCG TGTCTGGCGG TAGCAACTGC GGTGCCTCCT CTGACGCACT GTCTAGCGAT
    CTGCGACCTG CGAAGATTGG ACGCATGGTC CCCGCCGCAG CGGCAGTGGC AGCTGCGCCA
    ATGGGTGGCA AGACGACTAC TCGACAGCTA GACGAGAATG GAAATGCATT GGGCGATGGT
    TTCGGGGGCA GTGCAGCCAG TGGCTCCAGT GGCGCCAGTG GCGCCAGTGC TGCCGCCAGC
    AGTAGCAACG GCAACGGTAA CGCAAACCAG TTCACAGCTC TAATAACCCC CACGGTGGGA
    TCCAGTGTAC CCACCTCTAC TGTCGCTCAC CGTCAGAATA GTGTCTCCAC GCTGCTCTCC
    ACGGGCACCA ACACTACAAC AACGGCCATG GCAGCGGCGG CGGTGGCTGC ATCCTACAGC
    CAGTTGCAAC AGGCACCGGT CGGAGGCGCG GCTGGAACGG TTTCTGGGAT CGGCATTGGC
    ATGCAGCAGC CCACCAAGCA GAAGACGAAG AAGAAGTCGC AGCAGGAGCG CAACACCACC
    ACCGTCGATG TGGAGTCGGT TGCCGGCTAT AGGGGCAATG ATCCTGTTGA GCAGCTCGTC
    AAGTACATTG AAAACGATGT CAACGGTGGC GGAAACAGTG CCGCTGGACA GCGCAAGAAG
    GAGCGCAAGA AGCAAAACAA GCTCAAGAAG AGCAACTCGT TGGAGGAGCT GCGCACCTGC
    TCAAAAATGG AGGTGGACGA TTTGAAGCGC CAGTCGGCGA CCACGGAGAT GATGCGACAT
    AAGAAGGGCA CGAACAATGC ATCCTCGGGC GGATCGTCGT CGACGGCCTC TGGTTCCTCA
    AACAGTGGCA AGCACAACTC GGCATCCGTG GCGGATATTA ACAAAAACTG CAACAAGGAG
    CAGCAACAGC AATCGACGCC GACGGTGCAA GTGCGCAACA GCAACAATCC AGGAACGCAG
    CAGCGGAAGG GCGAGCGTCG GTCCTGGGGC ACCGAGGAGT TGCAGTACCT GGGTGACCAC
    CAGGAAATAT CCGCTGCCTG GTCGGAGCTG GAGACTGTGG GCCAGAAGGA GCTGCCGCTG
    GCGCTGCCCG CCTTGGCGCG CATGTCCGAA CTGGATGCCC TGAATACCGT CTTGTCGGAG
    ACCGCTGAGT TCCATGTGGT TACCAAGAAG AAGAAACCAA AGAAGCAGCG TGCTGTTACC
    ATGGACGATG CGGCGGTGGC TGCAGCTGCC GTCACGGGCG GCAACTTACA GCGCATGCAG
    CAGATCACCA AGTCCGCGTC CTCCAACATC ATGTCCCAGC GGATGCACTA CTATACTCCG
    TACAACAGCA ACAATGGTGG AGGCAGTGTC AACTATAAGC AACAGCAGCA ACAGAGCCAA
    CAGTACCAGG AGCACTACCA GGCGCCACAG GGGCAGCACC ACCATCACCA CCATCATCAT
    CACCACCATC ATCACCATCA CAGCGGCTCG GCGGTTTCGA ATCAGGTGGA TAGCTCACGG
    CGCAAGTCCA CGTCGTCTAT GCCCCCATCG GAGAAATCTG ACTCCAGTGA TCTCGACTCG
    GTCCACTCCC TGCCCATCCA GACGGGGAAG AAGAAGAGCC TAGGCGGTGG CAACAACAAA
    CAACGGGGGG CGCCGTCAGC CAGTCAACGC CAGGCCAAGC AAAACAACAA TGCTGCAGCC
    CCCATTTCGT ATGCGGATAT CGCCCGGAAC AAGCAGGAGG CTCTAAACAA TGCCACGGCC
    AGCGACACCG AGTTGGAGCT GGGCGACAAG ATGCAGATCA AGAGTGGCGG CAAATCCAAG
    TCGCGACCCG ATTTCCCGGA GCTGCCAGGT GCTGTTACGG CAGTGGCTGC CAGTGGAGGT
    GCAGCCAATC AGGCGACGGT TGTCAGCAGC CAAAATACGC CACCATCCTC GTCCAGTTCC
    ATCAGTTATT CGCAGAGTCT CAATGCAACG CCGCCCAGCA GCAGCAGCGA TGCGGAGTCC
    ACCAACTCGC CGGAGCTGCT GGCGCAGGTG CAGATACCGG CTTACAGCAT GATCACGCCA
    ACTCTAACAG CCTCCATGAT CAGCAGTGTC AGCAGTTCTG TCGCCAGCAC CTCTTCCACA
    TCCTGCTCAC CGCCAGCTCT GCAGAAATCC AAGAGCGTGG AGCACGACAC CAGCTACAGC
    TGCAATAGTA GCAACTTGGA TCAACAGTAT CCGGCACTAG AAAAGACCGT CAAGCGGCAT
    AGCACCAACA ACGTGTCTGT GGCCGTCGCA GCCTCCACCT CGGCATCGGC GTCCTCCTCG
    TTGTACAACT TTGCAGCGGC AGCCAAGCAG CAGCTGACAG AGAACACCCA TTCGGCCGTA
    TCGCCGCCGG CAGCTGTAAC AACTACCTCA ACCGCAGTCG CAGCCTCAGC TCCAGCTCCA
    ACTACAGTTA CAGTTCCAGC TGCAGTTACA GTTCCTTGTT ATTCCTCATC CTCCACACAC
    GGCTCGACTC AAACTTCAAC TTCTACTGCA TCGTCTGCTA TAGCAGCTTT ACCCTCGGGC
    AAGGCCAAGG CCAAGCCAAA GGAGCTATCT CCCAGCAGCA GTTGCAGCAA GAAGCCAACG
    AAGCCTAGTC AAGATGTGCC GTCAATCAGT CTGTCCATTT CCACCACGCC AAGGGCGACG
    ACCAGCACCA GCACTACTAC TACACAAAAG ACCACGGCTG CCACGCAGAC CGAGGGAGCC
    AAAAAGCTAA TCGGCGGGAT CCACAAGCTG CCCAGTAGCA GTTGTATTGC GAAGGGAGCC
    GGAGCCGTTT TGGAGGCCAC CGCCGGCAGG CGTGCTGTTA TCATACTCAA CGATGATCGC
    GAAGCAGGCA GGAGCAACAA TGAGTTCATA TTCGGTGATT TCAACGAGGA TGAGCTAAAA
    CTCTTCGACG ACAACTTGGA TGATGAGGAG GAGGGCCAGC ACAAGCAGTC TTCAAACAAG
    AAACAGCAAA CTGAAGGGGA ATCTGATGTG ACCCAAGATG ACACCGAAGA CGTGGACCAA
    GATCTGGACA AGGAACTGGA ATGCCTGGGA CGACGACCAG AGAAGAAGCA AGAGCGACAG
    CAGGATATCA GCGCCAGCAG CGATCAGCAT CTGAATGATT CTGGTGCGGC TTCGGATGCA
    GTCAACAGCT CAGCCAGCCT TGACATGCTC TCAATTTCTG CAGAGGCGGC ACAATCGTCA
    CCTAATGCGG GGGCAACCGC TACCTCATCC AGCGTCGCCA GTCCCATTAA CTCGTCCACG
    CCCTCGGGTG CTTCGTCCGC ATCGGCGAGC ACGTCCACCT CATCGTCCTC GGTCTCGATT
    TCATCATCGT CGGGCGGCAA CGGTGCCGCT TGTCTTGCAT CCGTTTCCGG TATGCTTGGT
    GGAGTGGCCA ATCAGTCCGG GGACAGCGGC ATCTATGCTG CCGCCAACAC GTCTATTAAG
    GAGCACGTTA ACACTTTCCT AAGCAAGGCC AGCAGCAGCG AGGAGACGCT GCCGAGTCCC
    AACTCCATAT CGATGCAACA GCTGGAAACA TGCAACGACA TCGAGGCGGC CATCATAGCC
    GCAGCCCGTG CTGCCGCTGC TGCCCGGAGC ACCAGCTGCA GTCGCAGCAA CTCTCAGGAG
    CAGGATGCCT TGCAGCCGCA GGTCAAGACC AAGGCGACTC CCAATCCGGA GGCGAAGATG
    GTTCTGCCTG TGTTTGTGAC CTACAACAAC AACAACAACG ACGACGATGA CGACGAGGAG
    GACGACGAGA GCCTGCAAGA ACTGAGCTTC ATGGCCGACC TTAAGGCGGA TGCAGCCACG
    GAGGATATTG CAATGCAGCC GCCACCGCCT TCATGTCCGG CGATGATGAC CAACACGGAA
    CTAATCGTCG ACTACATCGC CAAAACCTGG AATGCCATTG CCAACAGTAA GTACGTAACG
    TACTACAACG AGCAAGAGCA GGAGCAGGAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_572152.2
    CDS596..6331
    Translation

    Target ORF information:

    RefSeq Version NM_131924.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twenty-four (tyf), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_131924.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGAACAATG AACAAACGCC GTCATCATCG GACACACCGG CGGATCGTGT GGCATCTGGT 
    GGAGCAGCTG CCAAACTGCC AACGATCATA CCGGACACGC CCATCGTCTC GATTGCCGCT
    GGCACGATGA GCGAGAGCAC CACGACGACC ACCGTCACCA AAACAACGCC GACGCCGTTC
    CCACAGGAGC AGCTTATGAC CATGATGTCA GACCATGTGG CGAAGGCAGA CGCGCAGCTT
    GACGAGGCCT GTAGGAACCA GGGGGCCAAA GACGAGGACA GCCAAGGAGC GGCACCAGTG
    GCTGCCAACC ATGTAGCCAC CAGCGGCAGT AGCAGCAGAG GCAATGACCC CAAGAAGTCA
    TCATCCCCTA GTAATAGCCA TAGCTCTGGT AACCACGCAG CCACGGCAGA CGCTGCCAAA
    GGATCCGCAG CAGAAGCAGC AGCAGGAGAT ACGTCCTTCT CGCAGGTCTT TGCCATGCTA
    AAGGATTCGC CCAGCGCTGA ACTGTCGCGA CCAAACCGCA GGGTACTCCA GTACGGAGCC
    ACTGCCAAGG GCAAGACCCA GGACGATCAG AATGTCGGCA AGCGACAGAA GAAGAAAAAG
    ACAAAATACG ATACCTGGGA AGATAGCGAT TTTAATGATC TGGACGACGC ATCATTGAAG
    AAACTTCTCG AAGAGGCCTA CTGGTATCGA AATCCTGGCG ACAGGAAGAA CAAGAGCGAG
    CGATTTCTGC AAATGCTCAA AAAGGCTGAG TACGACGAAG AGATCTCTTA TCGTGCCATC
    AAATCCTGCC TCACAATCAA CCCATCCGCA CTAACGTCCA GTGCAGCAAC AGGCTCGAGC
    GGCGGGAGCA GCAGCAGTGC AGGGCACAAC AGCAGCTATA ATTCCACCAA TCACCGGTCA
    ACGGATCCCG GCCAGCGGCA CAAGCAGGGC GGCTCCCTGC AGGATCTCGT TGAGGCGGCG
    CACCGCAGCG AACTGGCCAC AACCTCGGCG GCGGCGGCAG CAGCAGCGAC AGCGGCAGCA
    ACCCAACGTT TCAACTGTGA TTACCAGCTG AGTGGTCGCT CCAATCGCCG GCAGCAGCAG
    CAGCAACAGC AGCACAACCA AAACGCCACC TCCTCCGCAT CCGCATCCAA AAAGATCAAG
    AACTTAAACG TCTCGGGCCG GCAGCGCGAA GGAGGCAGCC TGCCCAGCAG TGTTAACTTT
    GAGCCGGCAT CGTCTGCCAT GGAAGCCAAG CAGAATAAGC AACAGGCTCA GCCTTCGACA
    GAAGCTGCAG GTGGCTCGTT TTCGGGGTCG GTCGGTAACA AGCACCACCA CCGCAGCAGC
    ACCGGCAGTT GCAGCAGCCT GATAACTCAA TCGCGCGATA CGGAGGCAAA CAATCAGTTC
    GACTTGGACA TGGACGATGC GACGGGTGTT GGTGCCAGCG CCGTTGTCGG TGCCAGCGGC
    GACCAAGACG ACAACATGTT GGAGCAATTG GATCCTCAGA ACCCCTCGAT GAAGTTTCTG
    ATTGACGCCC TAAACGACAC GCCATTGGAG GCCAGGTACA CCGGTGCCGG CAAAAAGTTC
    CTAATCGACG ACCGGTTGCA TTTTTCCGTC CTGGAGCTGT CTGCCAGGAG GAAGCTGCAG
    CAGCAGGCAC AATACCTGGC CGCCCTCACC GGACAATTTG GTCTCGGCCT GGAGGAGGAG
    AAGCGCAAGG TAGAGGCCGG CTACGTGTCG CTCAACGACA ACTACACGGC GTGCTCCTCA
    GTTTACGGCG GCGGTGCAGG TTCGCCAGCT GCCGTGGAGG GCAGCAAGCC CTCTACCTCC
    TCCTCGGCCG TGTCTGGCGG TAGCAACTGC GGTGCCTCCT CTGACGCACT GTCTAGCGAT
    CTGCGACCTG CGAAGATTGG ACGCATGGTC CCCGCCGCAG CGGCAGTGGC AGCTGCGCCA
    ATGGGTGGCA AGACGACTAC TCGACAGCTA GACGAGAATG GAAATGCATT GGGCGATGGT
    TTCGGGGGCA GTGCAGCCAG TGGCTCCAGT GGCGCCAGTG GCGCCAGTGC TGCCGCCAGC
    AGTAGCAACG GCAACGGTAA CGCAAACCAG TTCACAGCTC TAATAACCCC CACGGTGGGA
    TCCAGTGTAC CCACCTCTAC TGTCGCTCAC CGTCAGAATA GTGTCTCCAC GCTGCTCTCC
    ACGGGCACCA ACACTACAAC AACGGCCATG GCAGCGGCGG CGGTGGCTGC ATCCTACAGC
    CAGTTGCAAC AGGCACCGGT CGGAGGCGCG GCTGGAACGG TTTCTGGGAT CGGCATTGGC
    ATGCAGCAGC CCACCAAGCA GAAGACGAAG AAGAAGTCGC AGCAGGAGCG CAACACCACC
    ACCGTCGATG TGGAGTCGGT TGCCGGCTAT AGGGGCAATG ATCCTGTTGA GCAGCTCGTC
    AAGTACATTG AAAACGATGT CAACGGTGGC GGAAACAGTG CCGCTGGACA GCGCAAGAAG
    GAGCGCAAGA AGCAAAACAA GCTCAAGAAG AGCAACTCGT TGGAGGAGCT GCGCACCTGC
    TCAAAAATGG AGGTGGACGA TTTGAAGCGC CAGTCGGCGA CCACGGAGAT GATGCGACAT
    AAGAAGGGCA CGAACAATGC ATCCTCGGGC GGATCGTCGT CGACGGCCTC TGGTTCCTCA
    AACAGTGGCA AGCACAACTC GGCATCCGTG GCGGATATTA ACAAAAACTG CAACAAGGAG
    CAGCAACAGC AATCGACGCC GACGGTGCAA GTGCGCAACA GCAACAATCC AGGAACGCAG
    CAGCGGAAGG GCGAGCGTCG GTCCTGGGGC ACCGAGGAGT TGCAGTACCT GGGTGACCAC
    CAGGAAATAT CCGCTGCCTG GTCGGAGCTG GAGACTGTGG GCCAGAAGGA GCTGCCGCTG
    GCGCTGCCCG CCTTGGCGCG CATGTCCGAA CTGGATGCCC TGAATACCGT CTTGTCGGAG
    ACCGCTGAGT TCCATGTGGT TACCAAGAAG AAGAAACCAA AGAAGCAGCG TGCTGTTACC
    ATGGACGATG CGGCGGTGGC TGCAGCTGCC GTCACGGGCG GCAACTTACA GCGCATGCAG
    CAGATCACCA AGTCCGCGTC CTCCAACATC ATGTCCCAGC GGATGCACTA CTATACTCCG
    TACAACAGCA ACAATGGTGG AGGCAGTGTC AACTATAAGC AACAGCAGCA ACAGAGCCAA
    CAGTACCAGG AGCACTACCA GGCGCCACAG GGGCAGCACC ACCATCACCA CCATCATCAT
    CACCACCATC ATCACCATCA CAGCGGCTCG GCGGTTTCGA ATCAGGTGGA TAGCTCACGG
    CGCAAGTCCA CGTCGTCTAT GCCCCCATCG GAGAAATCTG ACTCCAGTGA TCTCGACTCG
    GTCCACTCCC TGCCCATCCA GACGGGGAAG AAGAAGAGCC TAGGCGGTGG CAACAACAAA
    CAACGGGGGG CGCCGTCAGC CAGTCAACGC CAGGCCAAGC AAAACAACAA TGCTGCAGCC
    CCCATTTCGT ATGCGGATAT CGCCCGGAAC AAGCAGGAGG CTCTAAACAA TGCCACGGCC
    AGCGACACCG AGTTGGAGCT GGGCGACAAG ATGCAGATCA AGAGTGGCGG CAAATCCAAG
    TCGCGACCCG ATTTCCCGGA GCTGCCAGGT GCTGTTACGG CAGTGGCTGC CAGTGGAGGT
    GCAGCCAATC AGGCGACGGT TGTCAGCAGC CAAAATACGC CACCATCCTC GTCCAGTTCC
    ATCAGTTATT CGCAGAGTCT CAATGCAACG CCGCCCAGCA GCAGCAGCGA TGCGGAGTCC
    ACCAACTCGC CGGAGCTGCT GGCGCAGGTG CAGATACCGG CTTACAGCAT GATCACGCCA
    ACTCTAACAG CCTCCATGAT CAGCAGTGTC AGCAGTTCTG TCGCCAGCAC CTCTTCCACA
    TCCTGCTCAC CGCCAGCTCT GCAGAAATCC AAGAGCGTGG AGCACGACAC CAGCTACAGC
    TGCAATAGTA GCAACTTGGA TCAACAGTAT CCGGCACTAG AAAAGACCGT CAAGCGGCAT
    AGCACCAACA ACGTGTCTGT GGCCGTCGCA GCCTCCACCT CGGCATCGGC GTCCTCCTCG
    TTGTACAACT TTGCAGCGGC AGCCAAGCAG CAGCTGACAG AGAACACCCA TTCGGCCGTA
    TCGCCGCCGG CAGCTGTAAC AACTACCTCA ACCGCAGTCG CAGCCTCAGC TCCAGCTCCA
    ACTACAGTTA CAGTTCCAGC TGCAGTTACA GTTCCTTGTT ATTCCTCATC CTCCACACAC
    GGCTCGACTC AAACTTCAAC TTCTACTGCA TCGTCTGCTA TAGCAGCTTT ACCCTCGGGC
    AAGGCCAAGG CCAAGCCAAA GGAGCTATCT CCCAGCAGCA GTTGCAGCAA GAAGCCAACG
    AAGCCTAGTC AAGATGTGCC GTCAATCAGT CTGTCCATTT CCACCACGCC AAGGGCGACG
    ACCAGCACCA GCACTACTAC TACACAAAAG ACCACGGCTG CCACGCAGAC CGAGGGAGCC
    AAAAAGCTAA TCGGCGGGAT CCACAAGCTG CCCAGTAGCA GTTGTATTGC GAAGGGAGCC
    GGAGCCGTTT TGGAGGCCAC CGCCGGCAGG CGTGCTGTTA TCATACTCAA CGATGATCGC
    GAAGCAGGCA GGAGCAACAA TGAGTTCATA TTCGGTGATT TCAACGAGGA TGAGCTAAAA
    CTCTTCGACG ACAACTTGGA TGATGAGGAG GAGGGCCAGC ACAAGCAGTC TTCAAACAAG
    AAACAGCAAA CTGAAGGGGA ATCTGATGTG ACCCAAGATG ACACCGAAGA CGTGGACCAA
    GATCTGGACA AGGAACTGGA ATGCCTGGGA CGACGACCAG AGAAGAAGCA AGAGCGACAG
    CAGGATATCA GCGCCAGCAG CGATCAGCAT CTGAATGATT CTGGTGCGGC TTCGGATGCA
    GTCAACAGCT CAGCCAGCCT TGACATGCTC TCAATTTCTG CAGAGGCGGC ACAATCGTCA
    CCTAATGCGG GGGCAACCGC TACCTCATCC AGCGTCGCCA GTCCCATTAA CTCGTCCACG
    CCCTCGGGTG CTTCGTCCGC ATCGGCGAGC ACGTCCACCT CATCGTCCTC GGTCTCGATT
    TCATCATCGT CGGGCGGCAA CGGTGCCGCT TGTCTTGCAT CCGTTTCCGG TATGCTTGGT
    GGAGTGGCCA ATCAGTCCGG GGACAGCGGC ATCTATGCTG CCGCCAACAC GTCTATTAAG
    GAGCACGTTA ACACTTTCCT AAGCAAGGCC AGCAGCAGCG AGGAGACGCT GCCGAGTCCC
    AACTCCATAT CGATGCAACA GCTGGAAACA TGCAACGACA TCGAGGCGGC CATCATAGCC
    GCAGCCCGTG CTGCCGCTGC TGCCCGGAGC ACCAGCTGCA GTCGCAGCAA CTCTCAGGAG
    CAGGATGCCT TGCAGCCGCA GGTCAAGACC AAGGCGACTC CCAATCCGGA GGCGAAGATG
    GTTCTGCCTG TGTTTGTGAC CTACAACAAC AACAACAACG ACGACGATGA CGACGAGGAG
    GACGACGAGA GCCTGCAAGA ACTGAGCTTC ATGGCCGACC TTAAGGCGGA TGCAGCCACG
    GAGGATATTG CAATGCAGCC GCCACCGCCT TCATGTCCGG CGATGATGAC CAACACGGAA
    CTAATCGTCG ACTACATCGC CAAAACCTGG AATGCCATTG CCAACAGTAA GTACGTAACG
    TACTACAACG AGCAAGAGCA GGAGCAGGAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00756
    Clone ID Related Accession (Same CDS sequence) NM_001169187.2 , NM_131924.3 , NM_001169186.2 , NM_001258590.2 , NM_001297924.2
    Accession Version NM_001169186.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twenty-four, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_001169186.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGAACAATG AACAAACGCC GTCATCATCG GACACACCGG CGGATCGTGT GGCATCTGGT 
    GGAGCAGCTG CCAAACTGCC AACGATCATA CCGGACACGC CCATCGTCTC GATTGCCGCT
    GGCACGATGA GCGAGAGCAC CACGACGACC ACCGTCACCA AAACAACGCC GACGCCGTTC
    CCACAGGAGC AGCTTATGAC CATGATGTCA GACCATGTGG CGAAGGCAGA CGCGCAGCTT
    GACGAGGCCT GTAGGAACCA GGGGGCCAAA GACGAGGACA GCCAAGGAGC GGCACCAGTG
    GCTGCCAACC ATGTAGCCAC CAGCGGCAGT AGCAGCAGAG GCAATGACCC CAAGAAGTCA
    TCATCCCCTA GTAATAGCCA TAGCTCTGGT AACCACGCAG CCACGGCAGA CGCTGCCAAA
    GGATCCGCAG CAGAAGCAGC AGCAGGAGAT ACGTCCTTCT CGCAGGTCTT TGCCATGCTA
    AAGGATTCGC CCAGCGCTGA ACTGTCGCGA CCAAACCGCA GGGTACTCCA GTACGGAGCC
    ACTGCCAAGG GCAAGACCCA GGACGATCAG AATGTCGGCA AGCGACAGAA GAAGAAAAAG
    ACAAAATACG ATACCTGGGA AGATAGCGAT TTTAATGATC TGGACGACGC ATCATTGAAG
    AAACTTCTCG AAGAGGCCTA CTGGTATCGA AATCCTGGCG ACAGGAAGAA CAAGAGCGAG
    CGATTTCTGC AAATGCTCAA AAAGGCTGAG TACGACGAAG AGATCTCTTA TCGTGCCATC
    AAATCCTGCC TCACAATCAA CCCATCCGCA CTAACGTCCA GTGCAGCAAC AGGCTCGAGC
    GGCGGGAGCA GCAGCAGTGC AGGGCACAAC AGCAGCTATA ATTCCACCAA TCACCGGTCA
    ACGGATCCCG GCCAGCGGCA CAAGCAGGGC GGCTCCCTGC AGGATCTCGT TGAGGCGGCG
    CACCGCAGCG AACTGGCCAC AACCTCGGCG GCGGCGGCAG CAGCAGCGAC AGCGGCAGCA
    ACCCAACGTT TCAACTGTGA TTACCAGCTG AGTGGTCGCT CCAATCGCCG GCAGCAGCAG
    CAGCAACAGC AGCACAACCA AAACGCCACC TCCTCCGCAT CCGCATCCAA AAAGATCAAG
    AACTTAAACG TCTCGGGCCG GCAGCGCGAA GGAGGCAGCC TGCCCAGCAG TGTTAACTTT
    GAGCCGGCAT CGTCTGCCAT GGAAGCCAAG CAGAATAAGC AACAGGCTCA GCCTTCGACA
    GAAGCTGCAG GTGGCTCGTT TTCGGGGTCG GTCGGTAACA AGCACCACCA CCGCAGCAGC
    ACCGGCAGTT GCAGCAGCCT GATAACTCAA TCGCGCGATA CGGAGGCAAA CAATCAGTTC
    GACTTGGACA TGGACGATGC GACGGGTGTT GGTGCCAGCG CCGTTGTCGG TGCCAGCGGC
    GACCAAGACG ACAACATGTT GGAGCAATTG GATCCTCAGA ACCCCTCGAT GAAGTTTCTG
    ATTGACGCCC TAAACGACAC GCCATTGGAG GCCAGGTACA CCGGTGCCGG CAAAAAGTTC
    CTAATCGACG ACCGGTTGCA TTTTTCCGTC CTGGAGCTGT CTGCCAGGAG GAAGCTGCAG
    CAGCAGGCAC AATACCTGGC CGCCCTCACC GGACAATTTG GTCTCGGCCT GGAGGAGGAG
    AAGCGCAAGG TAGAGGCCGG CTACGTGTCG CTCAACGACA ACTACACGGC GTGCTCCTCA
    GTTTACGGCG GCGGTGCAGG TTCGCCAGCT GCCGTGGAGG GCAGCAAGCC CTCTACCTCC
    TCCTCGGCCG TGTCTGGCGG TAGCAACTGC GGTGCCTCCT CTGACGCACT GTCTAGCGAT
    CTGCGACCTG CGAAGATTGG ACGCATGGTC CCCGCCGCAG CGGCAGTGGC AGCTGCGCCA
    ATGGGTGGCA AGACGACTAC TCGACAGCTA GACGAGAATG GAAATGCATT GGGCGATGGT
    TTCGGGGGCA GTGCAGCCAG TGGCTCCAGT GGCGCCAGTG GCGCCAGTGC TGCCGCCAGC
    AGTAGCAACG GCAACGGTAA CGCAAACCAG TTCACAGCTC TAATAACCCC CACGGTGGGA
    TCCAGTGTAC CCACCTCTAC TGTCGCTCAC CGTCAGAATA GTGTCTCCAC GCTGCTCTCC
    ACGGGCACCA ACACTACAAC AACGGCCATG GCAGCGGCGG CGGTGGCTGC ATCCTACAGC
    CAGTTGCAAC AGGCACCGGT CGGAGGCGCG GCTGGAACGG TTTCTGGGAT CGGCATTGGC
    ATGCAGCAGC CCACCAAGCA GAAGACGAAG AAGAAGTCGC AGCAGGAGCG CAACACCACC
    ACCGTCGATG TGGAGTCGGT TGCCGGCTAT AGGGGCAATG ATCCTGTTGA GCAGCTCGTC
    AAGTACATTG AAAACGATGT CAACGGTGGC GGAAACAGTG CCGCTGGACA GCGCAAGAAG
    GAGCGCAAGA AGCAAAACAA GCTCAAGAAG AGCAACTCGT TGGAGGAGCT GCGCACCTGC
    TCAAAAATGG AGGTGGACGA TTTGAAGCGC CAGTCGGCGA CCACGGAGAT GATGCGACAT
    AAGAAGGGCA CGAACAATGC ATCCTCGGGC GGATCGTCGT CGACGGCCTC TGGTTCCTCA
    AACAGTGGCA AGCACAACTC GGCATCCGTG GCGGATATTA ACAAAAACTG CAACAAGGAG
    CAGCAACAGC AATCGACGCC GACGGTGCAA GTGCGCAACA GCAACAATCC AGGAACGCAG
    CAGCGGAAGG GCGAGCGTCG GTCCTGGGGC ACCGAGGAGT TGCAGTACCT GGGTGACCAC
    CAGGAAATAT CCGCTGCCTG GTCGGAGCTG GAGACTGTGG GCCAGAAGGA GCTGCCGCTG
    GCGCTGCCCG CCTTGGCGCG CATGTCCGAA CTGGATGCCC TGAATACCGT CTTGTCGGAG
    ACCGCTGAGT TCCATGTGGT TACCAAGAAG AAGAAACCAA AGAAGCAGCG TGCTGTTACC
    ATGGACGATG CGGCGGTGGC TGCAGCTGCC GTCACGGGCG GCAACTTACA GCGCATGCAG
    CAGATCACCA AGTCCGCGTC CTCCAACATC ATGTCCCAGC GGATGCACTA CTATACTCCG
    TACAACAGCA ACAATGGTGG AGGCAGTGTC AACTATAAGC AACAGCAGCA ACAGAGCCAA
    CAGTACCAGG AGCACTACCA GGCGCCACAG GGGCAGCACC ACCATCACCA CCATCATCAT
    CACCACCATC ATCACCATCA CAGCGGCTCG GCGGTTTCGA ATCAGGTGGA TAGCTCACGG
    CGCAAGTCCA CGTCGTCTAT GCCCCCATCG GAGAAATCTG ACTCCAGTGA TCTCGACTCG
    GTCCACTCCC TGCCCATCCA GACGGGGAAG AAGAAGAGCC TAGGCGGTGG CAACAACAAA
    CAACGGGGGG CGCCGTCAGC CAGTCAACGC CAGGCCAAGC AAAACAACAA TGCTGCAGCC
    CCCATTTCGT ATGCGGATAT CGCCCGGAAC AAGCAGGAGG CTCTAAACAA TGCCACGGCC
    AGCGACACCG AGTTGGAGCT GGGCGACAAG ATGCAGATCA AGAGTGGCGG CAAATCCAAG
    TCGCGACCCG ATTTCCCGGA GCTGCCAGGT GCTGTTACGG CAGTGGCTGC CAGTGGAGGT
    GCAGCCAATC AGGCGACGGT TGTCAGCAGC CAAAATACGC CACCATCCTC GTCCAGTTCC
    ATCAGTTATT CGCAGAGTCT CAATGCAACG CCGCCCAGCA GCAGCAGCGA TGCGGAGTCC
    ACCAACTCGC CGGAGCTGCT GGCGCAGGTG CAGATACCGG CTTACAGCAT GATCACGCCA
    ACTCTAACAG CCTCCATGAT CAGCAGTGTC AGCAGTTCTG TCGCCAGCAC CTCTTCCACA
    TCCTGCTCAC CGCCAGCTCT GCAGAAATCC AAGAGCGTGG AGCACGACAC CAGCTACAGC
    TGCAATAGTA GCAACTTGGA TCAACAGTAT CCGGCACTAG AAAAGACCGT CAAGCGGCAT
    AGCACCAACA ACGTGTCTGT GGCCGTCGCA GCCTCCACCT CGGCATCGGC GTCCTCCTCG
    TTGTACAACT TTGCAGCGGC AGCCAAGCAG CAGCTGACAG AGAACACCCA TTCGGCCGTA
    TCGCCGCCGG CAGCTGTAAC AACTACCTCA ACCGCAGTCG CAGCCTCAGC TCCAGCTCCA
    ACTACAGTTA CAGTTCCAGC TGCAGTTACA GTTCCTTGTT ATTCCTCATC CTCCACACAC
    GGCTCGACTC AAACTTCAAC TTCTACTGCA TCGTCTGCTA TAGCAGCTTT ACCCTCGGGC
    AAGGCCAAGG CCAAGCCAAA GGAGCTATCT CCCAGCAGCA GTTGCAGCAA GAAGCCAACG
    AAGCCTAGTC AAGATGTGCC GTCAATCAGT CTGTCCATTT CCACCACGCC AAGGGCGACG
    ACCAGCACCA GCACTACTAC TACACAAAAG ACCACGGCTG CCACGCAGAC CGAGGGAGCC
    AAAAAGCTAA TCGGCGGGAT CCACAAGCTG CCCAGTAGCA GTTGTATTGC GAAGGGAGCC
    GGAGCCGTTT TGGAGGCCAC CGCCGGCAGG CGTGCTGTTA TCATACTCAA CGATGATCGC
    GAAGCAGGCA GGAGCAACAA TGAGTTCATA TTCGGTGATT TCAACGAGGA TGAGCTAAAA
    CTCTTCGACG ACAACTTGGA TGATGAGGAG GAGGGCCAGC ACAAGCAGTC TTCAAACAAG
    AAACAGCAAA CTGAAGGGGA ATCTGATGTG ACCCAAGATG ACACCGAAGA CGTGGACCAA
    GATCTGGACA AGGAACTGGA ATGCCTGGGA CGACGACCAG AGAAGAAGCA AGAGCGACAG
    CAGGATATCA GCGCCAGCAG CGATCAGCAT CTGAATGATT CTGGTGCGGC TTCGGATGCA
    GTCAACAGCT CAGCCAGCCT TGACATGCTC TCAATTTCTG CAGAGGCGGC ACAATCGTCA
    CCTAATGCGG GGGCAACCGC TACCTCATCC AGCGTCGCCA GTCCCATTAA CTCGTCCACG
    CCCTCGGGTG CTTCGTCCGC ATCGGCGAGC ACGTCCACCT CATCGTCCTC GGTCTCGATT
    TCATCATCGT CGGGCGGCAA CGGTGCCGCT TGTCTTGCAT CCGTTTCCGG TATGCTTGGT
    GGAGTGGCCA ATCAGTCCGG GGACAGCGGC ATCTATGCTG CCGCCAACAC GTCTATTAAG
    GAGCACGTTA ACACTTTCCT AAGCAAGGCC AGCAGCAGCG AGGAGACGCT GCCGAGTCCC
    AACTCCATAT CGATGCAACA GCTGGAAACA TGCAACGACA TCGAGGCGGC CATCATAGCC
    GCAGCCCGTG CTGCCGCTGC TGCCCGGAGC ACCAGCTGCA GTCGCAGCAA CTCTCAGGAG
    CAGGATGCCT TGCAGCCGCA GGTCAAGACC AAGGCGACTC CCAATCCGGA GGCGAAGATG
    GTTCTGCCTG TGTTTGTGAC CTACAACAAC AACAACAACG ACGACGATGA CGACGAGGAG
    GACGACGAGA GCCTGCAAGA ACTGAGCTTC ATGGCCGACC TTAAGGCGGA TGCAGCCACG
    GAGGATATTG CAATGCAGCC GCCACCGCCT TCATGTCCGG CGATGATGAC CAACACGGAA
    CTAATCGTCG ACTACATCGC CAAAACCTGG AATGCCATTG CCAACAGTAA GTACGTAACG
    TACTACAACG AGCAAGAGCA GGAGCAGGAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162657.1
    CDS641..6376
    Translation

    Target ORF information:

    RefSeq Version NM_001169186.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twenty-four (tyf), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169186.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGAACAATG AACAAACGCC GTCATCATCG GACACACCGG CGGATCGTGT GGCATCTGGT 
    GGAGCAGCTG CCAAACTGCC AACGATCATA CCGGACACGC CCATCGTCTC GATTGCCGCT
    GGCACGATGA GCGAGAGCAC CACGACGACC ACCGTCACCA AAACAACGCC GACGCCGTTC
    CCACAGGAGC AGCTTATGAC CATGATGTCA GACCATGTGG CGAAGGCAGA CGCGCAGCTT
    GACGAGGCCT GTAGGAACCA GGGGGCCAAA GACGAGGACA GCCAAGGAGC GGCACCAGTG
    GCTGCCAACC ATGTAGCCAC CAGCGGCAGT AGCAGCAGAG GCAATGACCC CAAGAAGTCA
    TCATCCCCTA GTAATAGCCA TAGCTCTGGT AACCACGCAG CCACGGCAGA CGCTGCCAAA
    GGATCCGCAG CAGAAGCAGC AGCAGGAGAT ACGTCCTTCT CGCAGGTCTT TGCCATGCTA
    AAGGATTCGC CCAGCGCTGA ACTGTCGCGA CCAAACCGCA GGGTACTCCA GTACGGAGCC
    ACTGCCAAGG GCAAGACCCA GGACGATCAG AATGTCGGCA AGCGACAGAA GAAGAAAAAG
    ACAAAATACG ATACCTGGGA AGATAGCGAT TTTAATGATC TGGACGACGC ATCATTGAAG
    AAACTTCTCG AAGAGGCCTA CTGGTATCGA AATCCTGGCG ACAGGAAGAA CAAGAGCGAG
    CGATTTCTGC AAATGCTCAA AAAGGCTGAG TACGACGAAG AGATCTCTTA TCGTGCCATC
    AAATCCTGCC TCACAATCAA CCCATCCGCA CTAACGTCCA GTGCAGCAAC AGGCTCGAGC
    GGCGGGAGCA GCAGCAGTGC AGGGCACAAC AGCAGCTATA ATTCCACCAA TCACCGGTCA
    ACGGATCCCG GCCAGCGGCA CAAGCAGGGC GGCTCCCTGC AGGATCTCGT TGAGGCGGCG
    CACCGCAGCG AACTGGCCAC AACCTCGGCG GCGGCGGCAG CAGCAGCGAC AGCGGCAGCA
    ACCCAACGTT TCAACTGTGA TTACCAGCTG AGTGGTCGCT CCAATCGCCG GCAGCAGCAG
    CAGCAACAGC AGCACAACCA AAACGCCACC TCCTCCGCAT CCGCATCCAA AAAGATCAAG
    AACTTAAACG TCTCGGGCCG GCAGCGCGAA GGAGGCAGCC TGCCCAGCAG TGTTAACTTT
    GAGCCGGCAT CGTCTGCCAT GGAAGCCAAG CAGAATAAGC AACAGGCTCA GCCTTCGACA
    GAAGCTGCAG GTGGCTCGTT TTCGGGGTCG GTCGGTAACA AGCACCACCA CCGCAGCAGC
    ACCGGCAGTT GCAGCAGCCT GATAACTCAA TCGCGCGATA CGGAGGCAAA CAATCAGTTC
    GACTTGGACA TGGACGATGC GACGGGTGTT GGTGCCAGCG CCGTTGTCGG TGCCAGCGGC
    GACCAAGACG ACAACATGTT GGAGCAATTG GATCCTCAGA ACCCCTCGAT GAAGTTTCTG
    ATTGACGCCC TAAACGACAC GCCATTGGAG GCCAGGTACA CCGGTGCCGG CAAAAAGTTC
    CTAATCGACG ACCGGTTGCA TTTTTCCGTC CTGGAGCTGT CTGCCAGGAG GAAGCTGCAG
    CAGCAGGCAC AATACCTGGC CGCCCTCACC GGACAATTTG GTCTCGGCCT GGAGGAGGAG
    AAGCGCAAGG TAGAGGCCGG CTACGTGTCG CTCAACGACA ACTACACGGC GTGCTCCTCA
    GTTTACGGCG GCGGTGCAGG TTCGCCAGCT GCCGTGGAGG GCAGCAAGCC CTCTACCTCC
    TCCTCGGCCG TGTCTGGCGG TAGCAACTGC GGTGCCTCCT CTGACGCACT GTCTAGCGAT
    CTGCGACCTG CGAAGATTGG ACGCATGGTC CCCGCCGCAG CGGCAGTGGC AGCTGCGCCA
    ATGGGTGGCA AGACGACTAC TCGACAGCTA GACGAGAATG GAAATGCATT GGGCGATGGT
    TTCGGGGGCA GTGCAGCCAG TGGCTCCAGT GGCGCCAGTG GCGCCAGTGC TGCCGCCAGC
    AGTAGCAACG GCAACGGTAA CGCAAACCAG TTCACAGCTC TAATAACCCC CACGGTGGGA
    TCCAGTGTAC CCACCTCTAC TGTCGCTCAC CGTCAGAATA GTGTCTCCAC GCTGCTCTCC
    ACGGGCACCA ACACTACAAC AACGGCCATG GCAGCGGCGG CGGTGGCTGC ATCCTACAGC
    CAGTTGCAAC AGGCACCGGT CGGAGGCGCG GCTGGAACGG TTTCTGGGAT CGGCATTGGC
    ATGCAGCAGC CCACCAAGCA GAAGACGAAG AAGAAGTCGC AGCAGGAGCG CAACACCACC
    ACCGTCGATG TGGAGTCGGT TGCCGGCTAT AGGGGCAATG ATCCTGTTGA GCAGCTCGTC
    AAGTACATTG AAAACGATGT CAACGGTGGC GGAAACAGTG CCGCTGGACA GCGCAAGAAG
    GAGCGCAAGA AGCAAAACAA GCTCAAGAAG AGCAACTCGT TGGAGGAGCT GCGCACCTGC
    TCAAAAATGG AGGTGGACGA TTTGAAGCGC CAGTCGGCGA CCACGGAGAT GATGCGACAT
    AAGAAGGGCA CGAACAATGC ATCCTCGGGC GGATCGTCGT CGACGGCCTC TGGTTCCTCA
    AACAGTGGCA AGCACAACTC GGCATCCGTG GCGGATATTA ACAAAAACTG CAACAAGGAG
    CAGCAACAGC AATCGACGCC GACGGTGCAA GTGCGCAACA GCAACAATCC AGGAACGCAG
    CAGCGGAAGG GCGAGCGTCG GTCCTGGGGC ACCGAGGAGT TGCAGTACCT GGGTGACCAC
    CAGGAAATAT CCGCTGCCTG GTCGGAGCTG GAGACTGTGG GCCAGAAGGA GCTGCCGCTG
    GCGCTGCCCG CCTTGGCGCG CATGTCCGAA CTGGATGCCC TGAATACCGT CTTGTCGGAG
    ACCGCTGAGT TCCATGTGGT TACCAAGAAG AAGAAACCAA AGAAGCAGCG TGCTGTTACC
    ATGGACGATG CGGCGGTGGC TGCAGCTGCC GTCACGGGCG GCAACTTACA GCGCATGCAG
    CAGATCACCA AGTCCGCGTC CTCCAACATC ATGTCCCAGC GGATGCACTA CTATACTCCG
    TACAACAGCA ACAATGGTGG AGGCAGTGTC AACTATAAGC AACAGCAGCA ACAGAGCCAA
    CAGTACCAGG AGCACTACCA GGCGCCACAG GGGCAGCACC ACCATCACCA CCATCATCAT
    CACCACCATC ATCACCATCA CAGCGGCTCG GCGGTTTCGA ATCAGGTGGA TAGCTCACGG
    CGCAAGTCCA CGTCGTCTAT GCCCCCATCG GAGAAATCTG ACTCCAGTGA TCTCGACTCG
    GTCCACTCCC TGCCCATCCA GACGGGGAAG AAGAAGAGCC TAGGCGGTGG CAACAACAAA
    CAACGGGGGG CGCCGTCAGC CAGTCAACGC CAGGCCAAGC AAAACAACAA TGCTGCAGCC
    CCCATTTCGT ATGCGGATAT CGCCCGGAAC AAGCAGGAGG CTCTAAACAA TGCCACGGCC
    AGCGACACCG AGTTGGAGCT GGGCGACAAG ATGCAGATCA AGAGTGGCGG CAAATCCAAG
    TCGCGACCCG ATTTCCCGGA GCTGCCAGGT GCTGTTACGG CAGTGGCTGC CAGTGGAGGT
    GCAGCCAATC AGGCGACGGT TGTCAGCAGC CAAAATACGC CACCATCCTC GTCCAGTTCC
    ATCAGTTATT CGCAGAGTCT CAATGCAACG CCGCCCAGCA GCAGCAGCGA TGCGGAGTCC
    ACCAACTCGC CGGAGCTGCT GGCGCAGGTG CAGATACCGG CTTACAGCAT GATCACGCCA
    ACTCTAACAG CCTCCATGAT CAGCAGTGTC AGCAGTTCTG TCGCCAGCAC CTCTTCCACA
    TCCTGCTCAC CGCCAGCTCT GCAGAAATCC AAGAGCGTGG AGCACGACAC CAGCTACAGC
    TGCAATAGTA GCAACTTGGA TCAACAGTAT CCGGCACTAG AAAAGACCGT CAAGCGGCAT
    AGCACCAACA ACGTGTCTGT GGCCGTCGCA GCCTCCACCT CGGCATCGGC GTCCTCCTCG
    TTGTACAACT TTGCAGCGGC AGCCAAGCAG CAGCTGACAG AGAACACCCA TTCGGCCGTA
    TCGCCGCCGG CAGCTGTAAC AACTACCTCA ACCGCAGTCG CAGCCTCAGC TCCAGCTCCA
    ACTACAGTTA CAGTTCCAGC TGCAGTTACA GTTCCTTGTT ATTCCTCATC CTCCACACAC
    GGCTCGACTC AAACTTCAAC TTCTACTGCA TCGTCTGCTA TAGCAGCTTT ACCCTCGGGC
    AAGGCCAAGG CCAAGCCAAA GGAGCTATCT CCCAGCAGCA GTTGCAGCAA GAAGCCAACG
    AAGCCTAGTC AAGATGTGCC GTCAATCAGT CTGTCCATTT CCACCACGCC AAGGGCGACG
    ACCAGCACCA GCACTACTAC TACACAAAAG ACCACGGCTG CCACGCAGAC CGAGGGAGCC
    AAAAAGCTAA TCGGCGGGAT CCACAAGCTG CCCAGTAGCA GTTGTATTGC GAAGGGAGCC
    GGAGCCGTTT TGGAGGCCAC CGCCGGCAGG CGTGCTGTTA TCATACTCAA CGATGATCGC
    GAAGCAGGCA GGAGCAACAA TGAGTTCATA TTCGGTGATT TCAACGAGGA TGAGCTAAAA
    CTCTTCGACG ACAACTTGGA TGATGAGGAG GAGGGCCAGC ACAAGCAGTC TTCAAACAAG
    AAACAGCAAA CTGAAGGGGA ATCTGATGTG ACCCAAGATG ACACCGAAGA CGTGGACCAA
    GATCTGGACA AGGAACTGGA ATGCCTGGGA CGACGACCAG AGAAGAAGCA AGAGCGACAG
    CAGGATATCA GCGCCAGCAG CGATCAGCAT CTGAATGATT CTGGTGCGGC TTCGGATGCA
    GTCAACAGCT CAGCCAGCCT TGACATGCTC TCAATTTCTG CAGAGGCGGC ACAATCGTCA
    CCTAATGCGG GGGCAACCGC TACCTCATCC AGCGTCGCCA GTCCCATTAA CTCGTCCACG
    CCCTCGGGTG CTTCGTCCGC ATCGGCGAGC ACGTCCACCT CATCGTCCTC GGTCTCGATT
    TCATCATCGT CGGGCGGCAA CGGTGCCGCT TGTCTTGCAT CCGTTTCCGG TATGCTTGGT
    GGAGTGGCCA ATCAGTCCGG GGACAGCGGC ATCTATGCTG CCGCCAACAC GTCTATTAAG
    GAGCACGTTA ACACTTTCCT AAGCAAGGCC AGCAGCAGCG AGGAGACGCT GCCGAGTCCC
    AACTCCATAT CGATGCAACA GCTGGAAACA TGCAACGACA TCGAGGCGGC CATCATAGCC
    GCAGCCCGTG CTGCCGCTGC TGCCCGGAGC ACCAGCTGCA GTCGCAGCAA CTCTCAGGAG
    CAGGATGCCT TGCAGCCGCA GGTCAAGACC AAGGCGACTC CCAATCCGGA GGCGAAGATG
    GTTCTGCCTG TGTTTGTGAC CTACAACAAC AACAACAACG ACGACGATGA CGACGAGGAG
    GACGACGAGA GCCTGCAAGA ACTGAGCTTC ATGGCCGACC TTAAGGCGGA TGCAGCCACG
    GAGGATATTG CAATGCAGCC GCCACCGCCT TCATGTCCGG CGATGATGAC CAACACGGAA
    CTAATCGTCG ACTACATCGC CAAAACCTGG AATGCCATTG CCAACAGTAA GTACGTAACG
    TACTACAACG AGCAAGAGCA GGAGCAGGAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00756
    Clone ID Related Accession (Same CDS sequence) NM_001169187.2 , NM_131924.3 , NM_001169186.2 , NM_001258590.2 , NM_001297924.2
    Accession Version NM_001258590.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twenty-four, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_001258590.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGAACAATG AACAAACGCC GTCATCATCG GACACACCGG CGGATCGTGT GGCATCTGGT 
    GGAGCAGCTG CCAAACTGCC AACGATCATA CCGGACACGC CCATCGTCTC GATTGCCGCT
    GGCACGATGA GCGAGAGCAC CACGACGACC ACCGTCACCA AAACAACGCC GACGCCGTTC
    CCACAGGAGC AGCTTATGAC CATGATGTCA GACCATGTGG CGAAGGCAGA CGCGCAGCTT
    GACGAGGCCT GTAGGAACCA GGGGGCCAAA GACGAGGACA GCCAAGGAGC GGCACCAGTG
    GCTGCCAACC ATGTAGCCAC CAGCGGCAGT AGCAGCAGAG GCAATGACCC CAAGAAGTCA
    TCATCCCCTA GTAATAGCCA TAGCTCTGGT AACCACGCAG CCACGGCAGA CGCTGCCAAA
    GGATCCGCAG CAGAAGCAGC AGCAGGAGAT ACGTCCTTCT CGCAGGTCTT TGCCATGCTA
    AAGGATTCGC CCAGCGCTGA ACTGTCGCGA CCAAACCGCA GGGTACTCCA GTACGGAGCC
    ACTGCCAAGG GCAAGACCCA GGACGATCAG AATGTCGGCA AGCGACAGAA GAAGAAAAAG
    ACAAAATACG ATACCTGGGA AGATAGCGAT TTTAATGATC TGGACGACGC ATCATTGAAG
    AAACTTCTCG AAGAGGCCTA CTGGTATCGA AATCCTGGCG ACAGGAAGAA CAAGAGCGAG
    CGATTTCTGC AAATGCTCAA AAAGGCTGAG TACGACGAAG AGATCTCTTA TCGTGCCATC
    AAATCCTGCC TCACAATCAA CCCATCCGCA CTAACGTCCA GTGCAGCAAC AGGCTCGAGC
    GGCGGGAGCA GCAGCAGTGC AGGGCACAAC AGCAGCTATA ATTCCACCAA TCACCGGTCA
    ACGGATCCCG GCCAGCGGCA CAAGCAGGGC GGCTCCCTGC AGGATCTCGT TGAGGCGGCG
    CACCGCAGCG AACTGGCCAC AACCTCGGCG GCGGCGGCAG CAGCAGCGAC AGCGGCAGCA
    ACCCAACGTT TCAACTGTGA TTACCAGCTG AGTGGTCGCT CCAATCGCCG GCAGCAGCAG
    CAGCAACAGC AGCACAACCA AAACGCCACC TCCTCCGCAT CCGCATCCAA AAAGATCAAG
    AACTTAAACG TCTCGGGCCG GCAGCGCGAA GGAGGCAGCC TGCCCAGCAG TGTTAACTTT
    GAGCCGGCAT CGTCTGCCAT GGAAGCCAAG CAGAATAAGC AACAGGCTCA GCCTTCGACA
    GAAGCTGCAG GTGGCTCGTT TTCGGGGTCG GTCGGTAACA AGCACCACCA CCGCAGCAGC
    ACCGGCAGTT GCAGCAGCCT GATAACTCAA TCGCGCGATA CGGAGGCAAA CAATCAGTTC
    GACTTGGACA TGGACGATGC GACGGGTGTT GGTGCCAGCG CCGTTGTCGG TGCCAGCGGC
    GACCAAGACG ACAACATGTT GGAGCAATTG GATCCTCAGA ACCCCTCGAT GAAGTTTCTG
    ATTGACGCCC TAAACGACAC GCCATTGGAG GCCAGGTACA CCGGTGCCGG CAAAAAGTTC
    CTAATCGACG ACCGGTTGCA TTTTTCCGTC CTGGAGCTGT CTGCCAGGAG GAAGCTGCAG
    CAGCAGGCAC AATACCTGGC CGCCCTCACC GGACAATTTG GTCTCGGCCT GGAGGAGGAG
    AAGCGCAAGG TAGAGGCCGG CTACGTGTCG CTCAACGACA ACTACACGGC GTGCTCCTCA
    GTTTACGGCG GCGGTGCAGG TTCGCCAGCT GCCGTGGAGG GCAGCAAGCC CTCTACCTCC
    TCCTCGGCCG TGTCTGGCGG TAGCAACTGC GGTGCCTCCT CTGACGCACT GTCTAGCGAT
    CTGCGACCTG CGAAGATTGG ACGCATGGTC CCCGCCGCAG CGGCAGTGGC AGCTGCGCCA
    ATGGGTGGCA AGACGACTAC TCGACAGCTA GACGAGAATG GAAATGCATT GGGCGATGGT
    TTCGGGGGCA GTGCAGCCAG TGGCTCCAGT GGCGCCAGTG GCGCCAGTGC TGCCGCCAGC
    AGTAGCAACG GCAACGGTAA CGCAAACCAG TTCACAGCTC TAATAACCCC CACGGTGGGA
    TCCAGTGTAC CCACCTCTAC TGTCGCTCAC CGTCAGAATA GTGTCTCCAC GCTGCTCTCC
    ACGGGCACCA ACACTACAAC AACGGCCATG GCAGCGGCGG CGGTGGCTGC ATCCTACAGC
    CAGTTGCAAC AGGCACCGGT CGGAGGCGCG GCTGGAACGG TTTCTGGGAT CGGCATTGGC
    ATGCAGCAGC CCACCAAGCA GAAGACGAAG AAGAAGTCGC AGCAGGAGCG CAACACCACC
    ACCGTCGATG TGGAGTCGGT TGCCGGCTAT AGGGGCAATG ATCCTGTTGA GCAGCTCGTC
    AAGTACATTG AAAACGATGT CAACGGTGGC GGAAACAGTG CCGCTGGACA GCGCAAGAAG
    GAGCGCAAGA AGCAAAACAA GCTCAAGAAG AGCAACTCGT TGGAGGAGCT GCGCACCTGC
    TCAAAAATGG AGGTGGACGA TTTGAAGCGC CAGTCGGCGA CCACGGAGAT GATGCGACAT
    AAGAAGGGCA CGAACAATGC ATCCTCGGGC GGATCGTCGT CGACGGCCTC TGGTTCCTCA
    AACAGTGGCA AGCACAACTC GGCATCCGTG GCGGATATTA ACAAAAACTG CAACAAGGAG
    CAGCAACAGC AATCGACGCC GACGGTGCAA GTGCGCAACA GCAACAATCC AGGAACGCAG
    CAGCGGAAGG GCGAGCGTCG GTCCTGGGGC ACCGAGGAGT TGCAGTACCT GGGTGACCAC
    CAGGAAATAT CCGCTGCCTG GTCGGAGCTG GAGACTGTGG GCCAGAAGGA GCTGCCGCTG
    GCGCTGCCCG CCTTGGCGCG CATGTCCGAA CTGGATGCCC TGAATACCGT CTTGTCGGAG
    ACCGCTGAGT TCCATGTGGT TACCAAGAAG AAGAAACCAA AGAAGCAGCG TGCTGTTACC
    ATGGACGATG CGGCGGTGGC TGCAGCTGCC GTCACGGGCG GCAACTTACA GCGCATGCAG
    CAGATCACCA AGTCCGCGTC CTCCAACATC ATGTCCCAGC GGATGCACTA CTATACTCCG
    TACAACAGCA ACAATGGTGG AGGCAGTGTC AACTATAAGC AACAGCAGCA ACAGAGCCAA
    CAGTACCAGG AGCACTACCA GGCGCCACAG GGGCAGCACC ACCATCACCA CCATCATCAT
    CACCACCATC ATCACCATCA CAGCGGCTCG GCGGTTTCGA ATCAGGTGGA TAGCTCACGG
    CGCAAGTCCA CGTCGTCTAT GCCCCCATCG GAGAAATCTG ACTCCAGTGA TCTCGACTCG
    GTCCACTCCC TGCCCATCCA GACGGGGAAG AAGAAGAGCC TAGGCGGTGG CAACAACAAA
    CAACGGGGGG CGCCGTCAGC CAGTCAACGC CAGGCCAAGC AAAACAACAA TGCTGCAGCC
    CCCATTTCGT ATGCGGATAT CGCCCGGAAC AAGCAGGAGG CTCTAAACAA TGCCACGGCC
    AGCGACACCG AGTTGGAGCT GGGCGACAAG ATGCAGATCA AGAGTGGCGG CAAATCCAAG
    TCGCGACCCG ATTTCCCGGA GCTGCCAGGT GCTGTTACGG CAGTGGCTGC CAGTGGAGGT
    GCAGCCAATC AGGCGACGGT TGTCAGCAGC CAAAATACGC CACCATCCTC GTCCAGTTCC
    ATCAGTTATT CGCAGAGTCT CAATGCAACG CCGCCCAGCA GCAGCAGCGA TGCGGAGTCC
    ACCAACTCGC CGGAGCTGCT GGCGCAGGTG CAGATACCGG CTTACAGCAT GATCACGCCA
    ACTCTAACAG CCTCCATGAT CAGCAGTGTC AGCAGTTCTG TCGCCAGCAC CTCTTCCACA
    TCCTGCTCAC CGCCAGCTCT GCAGAAATCC AAGAGCGTGG AGCACGACAC CAGCTACAGC
    TGCAATAGTA GCAACTTGGA TCAACAGTAT CCGGCACTAG AAAAGACCGT CAAGCGGCAT
    AGCACCAACA ACGTGTCTGT GGCCGTCGCA GCCTCCACCT CGGCATCGGC GTCCTCCTCG
    TTGTACAACT TTGCAGCGGC AGCCAAGCAG CAGCTGACAG AGAACACCCA TTCGGCCGTA
    TCGCCGCCGG CAGCTGTAAC AACTACCTCA ACCGCAGTCG CAGCCTCAGC TCCAGCTCCA
    ACTACAGTTA CAGTTCCAGC TGCAGTTACA GTTCCTTGTT ATTCCTCATC CTCCACACAC
    GGCTCGACTC AAACTTCAAC TTCTACTGCA TCGTCTGCTA TAGCAGCTTT ACCCTCGGGC
    AAGGCCAAGG CCAAGCCAAA GGAGCTATCT CCCAGCAGCA GTTGCAGCAA GAAGCCAACG
    AAGCCTAGTC AAGATGTGCC GTCAATCAGT CTGTCCATTT CCACCACGCC AAGGGCGACG
    ACCAGCACCA GCACTACTAC TACACAAAAG ACCACGGCTG CCACGCAGAC CGAGGGAGCC
    AAAAAGCTAA TCGGCGGGAT CCACAAGCTG CCCAGTAGCA GTTGTATTGC GAAGGGAGCC
    GGAGCCGTTT TGGAGGCCAC CGCCGGCAGG CGTGCTGTTA TCATACTCAA CGATGATCGC
    GAAGCAGGCA GGAGCAACAA TGAGTTCATA TTCGGTGATT TCAACGAGGA TGAGCTAAAA
    CTCTTCGACG ACAACTTGGA TGATGAGGAG GAGGGCCAGC ACAAGCAGTC TTCAAACAAG
    AAACAGCAAA CTGAAGGGGA ATCTGATGTG ACCCAAGATG ACACCGAAGA CGTGGACCAA
    GATCTGGACA AGGAACTGGA ATGCCTGGGA CGACGACCAG AGAAGAAGCA AGAGCGACAG
    CAGGATATCA GCGCCAGCAG CGATCAGCAT CTGAATGATT CTGGTGCGGC TTCGGATGCA
    GTCAACAGCT CAGCCAGCCT TGACATGCTC TCAATTTCTG CAGAGGCGGC ACAATCGTCA
    CCTAATGCGG GGGCAACCGC TACCTCATCC AGCGTCGCCA GTCCCATTAA CTCGTCCACG
    CCCTCGGGTG CTTCGTCCGC ATCGGCGAGC ACGTCCACCT CATCGTCCTC GGTCTCGATT
    TCATCATCGT CGGGCGGCAA CGGTGCCGCT TGTCTTGCAT CCGTTTCCGG TATGCTTGGT
    GGAGTGGCCA ATCAGTCCGG GGACAGCGGC ATCTATGCTG CCGCCAACAC GTCTATTAAG
    GAGCACGTTA ACACTTTCCT AAGCAAGGCC AGCAGCAGCG AGGAGACGCT GCCGAGTCCC
    AACTCCATAT CGATGCAACA GCTGGAAACA TGCAACGACA TCGAGGCGGC CATCATAGCC
    GCAGCCCGTG CTGCCGCTGC TGCCCGGAGC ACCAGCTGCA GTCGCAGCAA CTCTCAGGAG
    CAGGATGCCT TGCAGCCGCA GGTCAAGACC AAGGCGACTC CCAATCCGGA GGCGAAGATG
    GTTCTGCCTG TGTTTGTGAC CTACAACAAC AACAACAACG ACGACGATGA CGACGAGGAG
    GACGACGAGA GCCTGCAAGA ACTGAGCTTC ATGGCCGACC TTAAGGCGGA TGCAGCCACG
    GAGGATATTG CAATGCAGCC GCCACCGCCT TCATGTCCGG CGATGATGAC CAACACGGAA
    CTAATCGTCG ACTACATCGC CAAAACCTGG AATGCCATTG CCAACAGTAA GTACGTAACG
    TACTACAACG AGCAAGAGCA GGAGCAGGAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245519.1
    CDS725..6460
    Translation

    Target ORF information:

    RefSeq Version NM_001258590.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twenty-four (tyf), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258590.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGAACAATG AACAAACGCC GTCATCATCG GACACACCGG CGGATCGTGT GGCATCTGGT 
    GGAGCAGCTG CCAAACTGCC AACGATCATA CCGGACACGC CCATCGTCTC GATTGCCGCT
    GGCACGATGA GCGAGAGCAC CACGACGACC ACCGTCACCA AAACAACGCC GACGCCGTTC
    CCACAGGAGC AGCTTATGAC CATGATGTCA GACCATGTGG CGAAGGCAGA CGCGCAGCTT
    GACGAGGCCT GTAGGAACCA GGGGGCCAAA GACGAGGACA GCCAAGGAGC GGCACCAGTG
    GCTGCCAACC ATGTAGCCAC CAGCGGCAGT AGCAGCAGAG GCAATGACCC CAAGAAGTCA
    TCATCCCCTA GTAATAGCCA TAGCTCTGGT AACCACGCAG CCACGGCAGA CGCTGCCAAA
    GGATCCGCAG CAGAAGCAGC AGCAGGAGAT ACGTCCTTCT CGCAGGTCTT TGCCATGCTA
    AAGGATTCGC CCAGCGCTGA ACTGTCGCGA CCAAACCGCA GGGTACTCCA GTACGGAGCC
    ACTGCCAAGG GCAAGACCCA GGACGATCAG AATGTCGGCA AGCGACAGAA GAAGAAAAAG
    ACAAAATACG ATACCTGGGA AGATAGCGAT TTTAATGATC TGGACGACGC ATCATTGAAG
    AAACTTCTCG AAGAGGCCTA CTGGTATCGA AATCCTGGCG ACAGGAAGAA CAAGAGCGAG
    CGATTTCTGC AAATGCTCAA AAAGGCTGAG TACGACGAAG AGATCTCTTA TCGTGCCATC
    AAATCCTGCC TCACAATCAA CCCATCCGCA CTAACGTCCA GTGCAGCAAC AGGCTCGAGC
    GGCGGGAGCA GCAGCAGTGC AGGGCACAAC AGCAGCTATA ATTCCACCAA TCACCGGTCA
    ACGGATCCCG GCCAGCGGCA CAAGCAGGGC GGCTCCCTGC AGGATCTCGT TGAGGCGGCG
    CACCGCAGCG AACTGGCCAC AACCTCGGCG GCGGCGGCAG CAGCAGCGAC AGCGGCAGCA
    ACCCAACGTT TCAACTGTGA TTACCAGCTG AGTGGTCGCT CCAATCGCCG GCAGCAGCAG
    CAGCAACAGC AGCACAACCA AAACGCCACC TCCTCCGCAT CCGCATCCAA AAAGATCAAG
    AACTTAAACG TCTCGGGCCG GCAGCGCGAA GGAGGCAGCC TGCCCAGCAG TGTTAACTTT
    GAGCCGGCAT CGTCTGCCAT GGAAGCCAAG CAGAATAAGC AACAGGCTCA GCCTTCGACA
    GAAGCTGCAG GTGGCTCGTT TTCGGGGTCG GTCGGTAACA AGCACCACCA CCGCAGCAGC
    ACCGGCAGTT GCAGCAGCCT GATAACTCAA TCGCGCGATA CGGAGGCAAA CAATCAGTTC
    GACTTGGACA TGGACGATGC GACGGGTGTT GGTGCCAGCG CCGTTGTCGG TGCCAGCGGC
    GACCAAGACG ACAACATGTT GGAGCAATTG GATCCTCAGA ACCCCTCGAT GAAGTTTCTG
    ATTGACGCCC TAAACGACAC GCCATTGGAG GCCAGGTACA CCGGTGCCGG CAAAAAGTTC
    CTAATCGACG ACCGGTTGCA TTTTTCCGTC CTGGAGCTGT CTGCCAGGAG GAAGCTGCAG
    CAGCAGGCAC AATACCTGGC CGCCCTCACC GGACAATTTG GTCTCGGCCT GGAGGAGGAG
    AAGCGCAAGG TAGAGGCCGG CTACGTGTCG CTCAACGACA ACTACACGGC GTGCTCCTCA
    GTTTACGGCG GCGGTGCAGG TTCGCCAGCT GCCGTGGAGG GCAGCAAGCC CTCTACCTCC
    TCCTCGGCCG TGTCTGGCGG TAGCAACTGC GGTGCCTCCT CTGACGCACT GTCTAGCGAT
    CTGCGACCTG CGAAGATTGG ACGCATGGTC CCCGCCGCAG CGGCAGTGGC AGCTGCGCCA
    ATGGGTGGCA AGACGACTAC TCGACAGCTA GACGAGAATG GAAATGCATT GGGCGATGGT
    TTCGGGGGCA GTGCAGCCAG TGGCTCCAGT GGCGCCAGTG GCGCCAGTGC TGCCGCCAGC
    AGTAGCAACG GCAACGGTAA CGCAAACCAG TTCACAGCTC TAATAACCCC CACGGTGGGA
    TCCAGTGTAC CCACCTCTAC TGTCGCTCAC CGTCAGAATA GTGTCTCCAC GCTGCTCTCC
    ACGGGCACCA ACACTACAAC AACGGCCATG GCAGCGGCGG CGGTGGCTGC ATCCTACAGC
    CAGTTGCAAC AGGCACCGGT CGGAGGCGCG GCTGGAACGG TTTCTGGGAT CGGCATTGGC
    ATGCAGCAGC CCACCAAGCA GAAGACGAAG AAGAAGTCGC AGCAGGAGCG CAACACCACC
    ACCGTCGATG TGGAGTCGGT TGCCGGCTAT AGGGGCAATG ATCCTGTTGA GCAGCTCGTC
    AAGTACATTG AAAACGATGT CAACGGTGGC GGAAACAGTG CCGCTGGACA GCGCAAGAAG
    GAGCGCAAGA AGCAAAACAA GCTCAAGAAG AGCAACTCGT TGGAGGAGCT GCGCACCTGC
    TCAAAAATGG AGGTGGACGA TTTGAAGCGC CAGTCGGCGA CCACGGAGAT GATGCGACAT
    AAGAAGGGCA CGAACAATGC ATCCTCGGGC GGATCGTCGT CGACGGCCTC TGGTTCCTCA
    AACAGTGGCA AGCACAACTC GGCATCCGTG GCGGATATTA ACAAAAACTG CAACAAGGAG
    CAGCAACAGC AATCGACGCC GACGGTGCAA GTGCGCAACA GCAACAATCC AGGAACGCAG
    CAGCGGAAGG GCGAGCGTCG GTCCTGGGGC ACCGAGGAGT TGCAGTACCT GGGTGACCAC
    CAGGAAATAT CCGCTGCCTG GTCGGAGCTG GAGACTGTGG GCCAGAAGGA GCTGCCGCTG
    GCGCTGCCCG CCTTGGCGCG CATGTCCGAA CTGGATGCCC TGAATACCGT CTTGTCGGAG
    ACCGCTGAGT TCCATGTGGT TACCAAGAAG AAGAAACCAA AGAAGCAGCG TGCTGTTACC
    ATGGACGATG CGGCGGTGGC TGCAGCTGCC GTCACGGGCG GCAACTTACA GCGCATGCAG
    CAGATCACCA AGTCCGCGTC CTCCAACATC ATGTCCCAGC GGATGCACTA CTATACTCCG
    TACAACAGCA ACAATGGTGG AGGCAGTGTC AACTATAAGC AACAGCAGCA ACAGAGCCAA
    CAGTACCAGG AGCACTACCA GGCGCCACAG GGGCAGCACC ACCATCACCA CCATCATCAT
    CACCACCATC ATCACCATCA CAGCGGCTCG GCGGTTTCGA ATCAGGTGGA TAGCTCACGG
    CGCAAGTCCA CGTCGTCTAT GCCCCCATCG GAGAAATCTG ACTCCAGTGA TCTCGACTCG
    GTCCACTCCC TGCCCATCCA GACGGGGAAG AAGAAGAGCC TAGGCGGTGG CAACAACAAA
    CAACGGGGGG CGCCGTCAGC CAGTCAACGC CAGGCCAAGC AAAACAACAA TGCTGCAGCC
    CCCATTTCGT ATGCGGATAT CGCCCGGAAC AAGCAGGAGG CTCTAAACAA TGCCACGGCC
    AGCGACACCG AGTTGGAGCT GGGCGACAAG ATGCAGATCA AGAGTGGCGG CAAATCCAAG
    TCGCGACCCG ATTTCCCGGA GCTGCCAGGT GCTGTTACGG CAGTGGCTGC CAGTGGAGGT
    GCAGCCAATC AGGCGACGGT TGTCAGCAGC CAAAATACGC CACCATCCTC GTCCAGTTCC
    ATCAGTTATT CGCAGAGTCT CAATGCAACG CCGCCCAGCA GCAGCAGCGA TGCGGAGTCC
    ACCAACTCGC CGGAGCTGCT GGCGCAGGTG CAGATACCGG CTTACAGCAT GATCACGCCA
    ACTCTAACAG CCTCCATGAT CAGCAGTGTC AGCAGTTCTG TCGCCAGCAC CTCTTCCACA
    TCCTGCTCAC CGCCAGCTCT GCAGAAATCC AAGAGCGTGG AGCACGACAC CAGCTACAGC
    TGCAATAGTA GCAACTTGGA TCAACAGTAT CCGGCACTAG AAAAGACCGT CAAGCGGCAT
    AGCACCAACA ACGTGTCTGT GGCCGTCGCA GCCTCCACCT CGGCATCGGC GTCCTCCTCG
    TTGTACAACT TTGCAGCGGC AGCCAAGCAG CAGCTGACAG AGAACACCCA TTCGGCCGTA
    TCGCCGCCGG CAGCTGTAAC AACTACCTCA ACCGCAGTCG CAGCCTCAGC TCCAGCTCCA
    ACTACAGTTA CAGTTCCAGC TGCAGTTACA GTTCCTTGTT ATTCCTCATC CTCCACACAC
    GGCTCGACTC AAACTTCAAC TTCTACTGCA TCGTCTGCTA TAGCAGCTTT ACCCTCGGGC
    AAGGCCAAGG CCAAGCCAAA GGAGCTATCT CCCAGCAGCA GTTGCAGCAA GAAGCCAACG
    AAGCCTAGTC AAGATGTGCC GTCAATCAGT CTGTCCATTT CCACCACGCC AAGGGCGACG
    ACCAGCACCA GCACTACTAC TACACAAAAG ACCACGGCTG CCACGCAGAC CGAGGGAGCC
    AAAAAGCTAA TCGGCGGGAT CCACAAGCTG CCCAGTAGCA GTTGTATTGC GAAGGGAGCC
    GGAGCCGTTT TGGAGGCCAC CGCCGGCAGG CGTGCTGTTA TCATACTCAA CGATGATCGC
    GAAGCAGGCA GGAGCAACAA TGAGTTCATA TTCGGTGATT TCAACGAGGA TGAGCTAAAA
    CTCTTCGACG ACAACTTGGA TGATGAGGAG GAGGGCCAGC ACAAGCAGTC TTCAAACAAG
    AAACAGCAAA CTGAAGGGGA ATCTGATGTG ACCCAAGATG ACACCGAAGA CGTGGACCAA
    GATCTGGACA AGGAACTGGA ATGCCTGGGA CGACGACCAG AGAAGAAGCA AGAGCGACAG
    CAGGATATCA GCGCCAGCAG CGATCAGCAT CTGAATGATT CTGGTGCGGC TTCGGATGCA
    GTCAACAGCT CAGCCAGCCT TGACATGCTC TCAATTTCTG CAGAGGCGGC ACAATCGTCA
    CCTAATGCGG GGGCAACCGC TACCTCATCC AGCGTCGCCA GTCCCATTAA CTCGTCCACG
    CCCTCGGGTG CTTCGTCCGC ATCGGCGAGC ACGTCCACCT CATCGTCCTC GGTCTCGATT
    TCATCATCGT CGGGCGGCAA CGGTGCCGCT TGTCTTGCAT CCGTTTCCGG TATGCTTGGT
    GGAGTGGCCA ATCAGTCCGG GGACAGCGGC ATCTATGCTG CCGCCAACAC GTCTATTAAG
    GAGCACGTTA ACACTTTCCT AAGCAAGGCC AGCAGCAGCG AGGAGACGCT GCCGAGTCCC
    AACTCCATAT CGATGCAACA GCTGGAAACA TGCAACGACA TCGAGGCGGC CATCATAGCC
    GCAGCCCGTG CTGCCGCTGC TGCCCGGAGC ACCAGCTGCA GTCGCAGCAA CTCTCAGGAG
    CAGGATGCCT TGCAGCCGCA GGTCAAGACC AAGGCGACTC CCAATCCGGA GGCGAAGATG
    GTTCTGCCTG TGTTTGTGAC CTACAACAAC AACAACAACG ACGACGATGA CGACGAGGAG
    GACGACGAGA GCCTGCAAGA ACTGAGCTTC ATGGCCGACC TTAAGGCGGA TGCAGCCACG
    GAGGATATTG CAATGCAGCC GCCACCGCCT TCATGTCCGG CGATGATGAC CAACACGGAA
    CTAATCGTCG ACTACATCGC CAAAACCTGG AATGCCATTG CCAACAGTAA GTACGTAACG
    TACTACAACG AGCAAGAGCA GGAGCAGGAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00756
    Clone ID Related Accession (Same CDS sequence) NM_001169187.2 , NM_131924.3 , NM_001169186.2 , NM_001258590.2 , NM_001297924.2
    Accession Version NM_001297924.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twenty-four, isoform N
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_001297924.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGAACAATG AACAAACGCC GTCATCATCG GACACACCGG CGGATCGTGT GGCATCTGGT 
    GGAGCAGCTG CCAAACTGCC AACGATCATA CCGGACACGC CCATCGTCTC GATTGCCGCT
    GGCACGATGA GCGAGAGCAC CACGACGACC ACCGTCACCA AAACAACGCC GACGCCGTTC
    CCACAGGAGC AGCTTATGAC CATGATGTCA GACCATGTGG CGAAGGCAGA CGCGCAGCTT
    GACGAGGCCT GTAGGAACCA GGGGGCCAAA GACGAGGACA GCCAAGGAGC GGCACCAGTG
    GCTGCCAACC ATGTAGCCAC CAGCGGCAGT AGCAGCAGAG GCAATGACCC CAAGAAGTCA
    TCATCCCCTA GTAATAGCCA TAGCTCTGGT AACCACGCAG CCACGGCAGA CGCTGCCAAA
    GGATCCGCAG CAGAAGCAGC AGCAGGAGAT ACGTCCTTCT CGCAGGTCTT TGCCATGCTA
    AAGGATTCGC CCAGCGCTGA ACTGTCGCGA CCAAACCGCA GGGTACTCCA GTACGGAGCC
    ACTGCCAAGG GCAAGACCCA GGACGATCAG AATGTCGGCA AGCGACAGAA GAAGAAAAAG
    ACAAAATACG ATACCTGGGA AGATAGCGAT TTTAATGATC TGGACGACGC ATCATTGAAG
    AAACTTCTCG AAGAGGCCTA CTGGTATCGA AATCCTGGCG ACAGGAAGAA CAAGAGCGAG
    CGATTTCTGC AAATGCTCAA AAAGGCTGAG TACGACGAAG AGATCTCTTA TCGTGCCATC
    AAATCCTGCC TCACAATCAA CCCATCCGCA CTAACGTCCA GTGCAGCAAC AGGCTCGAGC
    GGCGGGAGCA GCAGCAGTGC AGGGCACAAC AGCAGCTATA ATTCCACCAA TCACCGGTCA
    ACGGATCCCG GCCAGCGGCA CAAGCAGGGC GGCTCCCTGC AGGATCTCGT TGAGGCGGCG
    CACCGCAGCG AACTGGCCAC AACCTCGGCG GCGGCGGCAG CAGCAGCGAC AGCGGCAGCA
    ACCCAACGTT TCAACTGTGA TTACCAGCTG AGTGGTCGCT CCAATCGCCG GCAGCAGCAG
    CAGCAACAGC AGCACAACCA AAACGCCACC TCCTCCGCAT CCGCATCCAA AAAGATCAAG
    AACTTAAACG TCTCGGGCCG GCAGCGCGAA GGAGGCAGCC TGCCCAGCAG TGTTAACTTT
    GAGCCGGCAT CGTCTGCCAT GGAAGCCAAG CAGAATAAGC AACAGGCTCA GCCTTCGACA
    GAAGCTGCAG GTGGCTCGTT TTCGGGGTCG GTCGGTAACA AGCACCACCA CCGCAGCAGC
    ACCGGCAGTT GCAGCAGCCT GATAACTCAA TCGCGCGATA CGGAGGCAAA CAATCAGTTC
    GACTTGGACA TGGACGATGC GACGGGTGTT GGTGCCAGCG CCGTTGTCGG TGCCAGCGGC
    GACCAAGACG ACAACATGTT GGAGCAATTG GATCCTCAGA ACCCCTCGAT GAAGTTTCTG
    ATTGACGCCC TAAACGACAC GCCATTGGAG GCCAGGTACA CCGGTGCCGG CAAAAAGTTC
    CTAATCGACG ACCGGTTGCA TTTTTCCGTC CTGGAGCTGT CTGCCAGGAG GAAGCTGCAG
    CAGCAGGCAC AATACCTGGC CGCCCTCACC GGACAATTTG GTCTCGGCCT GGAGGAGGAG
    AAGCGCAAGG TAGAGGCCGG CTACGTGTCG CTCAACGACA ACTACACGGC GTGCTCCTCA
    GTTTACGGCG GCGGTGCAGG TTCGCCAGCT GCCGTGGAGG GCAGCAAGCC CTCTACCTCC
    TCCTCGGCCG TGTCTGGCGG TAGCAACTGC GGTGCCTCCT CTGACGCACT GTCTAGCGAT
    CTGCGACCTG CGAAGATTGG ACGCATGGTC CCCGCCGCAG CGGCAGTGGC AGCTGCGCCA
    ATGGGTGGCA AGACGACTAC TCGACAGCTA GACGAGAATG GAAATGCATT GGGCGATGGT
    TTCGGGGGCA GTGCAGCCAG TGGCTCCAGT GGCGCCAGTG GCGCCAGTGC TGCCGCCAGC
    AGTAGCAACG GCAACGGTAA CGCAAACCAG TTCACAGCTC TAATAACCCC CACGGTGGGA
    TCCAGTGTAC CCACCTCTAC TGTCGCTCAC CGTCAGAATA GTGTCTCCAC GCTGCTCTCC
    ACGGGCACCA ACACTACAAC AACGGCCATG GCAGCGGCGG CGGTGGCTGC ATCCTACAGC
    CAGTTGCAAC AGGCACCGGT CGGAGGCGCG GCTGGAACGG TTTCTGGGAT CGGCATTGGC
    ATGCAGCAGC CCACCAAGCA GAAGACGAAG AAGAAGTCGC AGCAGGAGCG CAACACCACC
    ACCGTCGATG TGGAGTCGGT TGCCGGCTAT AGGGGCAATG ATCCTGTTGA GCAGCTCGTC
    AAGTACATTG AAAACGATGT CAACGGTGGC GGAAACAGTG CCGCTGGACA GCGCAAGAAG
    GAGCGCAAGA AGCAAAACAA GCTCAAGAAG AGCAACTCGT TGGAGGAGCT GCGCACCTGC
    TCAAAAATGG AGGTGGACGA TTTGAAGCGC CAGTCGGCGA CCACGGAGAT GATGCGACAT
    AAGAAGGGCA CGAACAATGC ATCCTCGGGC GGATCGTCGT CGACGGCCTC TGGTTCCTCA
    AACAGTGGCA AGCACAACTC GGCATCCGTG GCGGATATTA ACAAAAACTG CAACAAGGAG
    CAGCAACAGC AATCGACGCC GACGGTGCAA GTGCGCAACA GCAACAATCC AGGAACGCAG
    CAGCGGAAGG GCGAGCGTCG GTCCTGGGGC ACCGAGGAGT TGCAGTACCT GGGTGACCAC
    CAGGAAATAT CCGCTGCCTG GTCGGAGCTG GAGACTGTGG GCCAGAAGGA GCTGCCGCTG
    GCGCTGCCCG CCTTGGCGCG CATGTCCGAA CTGGATGCCC TGAATACCGT CTTGTCGGAG
    ACCGCTGAGT TCCATGTGGT TACCAAGAAG AAGAAACCAA AGAAGCAGCG TGCTGTTACC
    ATGGACGATG CGGCGGTGGC TGCAGCTGCC GTCACGGGCG GCAACTTACA GCGCATGCAG
    CAGATCACCA AGTCCGCGTC CTCCAACATC ATGTCCCAGC GGATGCACTA CTATACTCCG
    TACAACAGCA ACAATGGTGG AGGCAGTGTC AACTATAAGC AACAGCAGCA ACAGAGCCAA
    CAGTACCAGG AGCACTACCA GGCGCCACAG GGGCAGCACC ACCATCACCA CCATCATCAT
    CACCACCATC ATCACCATCA CAGCGGCTCG GCGGTTTCGA ATCAGGTGGA TAGCTCACGG
    CGCAAGTCCA CGTCGTCTAT GCCCCCATCG GAGAAATCTG ACTCCAGTGA TCTCGACTCG
    GTCCACTCCC TGCCCATCCA GACGGGGAAG AAGAAGAGCC TAGGCGGTGG CAACAACAAA
    CAACGGGGGG CGCCGTCAGC CAGTCAACGC CAGGCCAAGC AAAACAACAA TGCTGCAGCC
    CCCATTTCGT ATGCGGATAT CGCCCGGAAC AAGCAGGAGG CTCTAAACAA TGCCACGGCC
    AGCGACACCG AGTTGGAGCT GGGCGACAAG ATGCAGATCA AGAGTGGCGG CAAATCCAAG
    TCGCGACCCG ATTTCCCGGA GCTGCCAGGT GCTGTTACGG CAGTGGCTGC CAGTGGAGGT
    GCAGCCAATC AGGCGACGGT TGTCAGCAGC CAAAATACGC CACCATCCTC GTCCAGTTCC
    ATCAGTTATT CGCAGAGTCT CAATGCAACG CCGCCCAGCA GCAGCAGCGA TGCGGAGTCC
    ACCAACTCGC CGGAGCTGCT GGCGCAGGTG CAGATACCGG CTTACAGCAT GATCACGCCA
    ACTCTAACAG CCTCCATGAT CAGCAGTGTC AGCAGTTCTG TCGCCAGCAC CTCTTCCACA
    TCCTGCTCAC CGCCAGCTCT GCAGAAATCC AAGAGCGTGG AGCACGACAC CAGCTACAGC
    TGCAATAGTA GCAACTTGGA TCAACAGTAT CCGGCACTAG AAAAGACCGT CAAGCGGCAT
    AGCACCAACA ACGTGTCTGT GGCCGTCGCA GCCTCCACCT CGGCATCGGC GTCCTCCTCG
    TTGTACAACT TTGCAGCGGC AGCCAAGCAG CAGCTGACAG AGAACACCCA TTCGGCCGTA
    TCGCCGCCGG CAGCTGTAAC AACTACCTCA ACCGCAGTCG CAGCCTCAGC TCCAGCTCCA
    ACTACAGTTA CAGTTCCAGC TGCAGTTACA GTTCCTTGTT ATTCCTCATC CTCCACACAC
    GGCTCGACTC AAACTTCAAC TTCTACTGCA TCGTCTGCTA TAGCAGCTTT ACCCTCGGGC
    AAGGCCAAGG CCAAGCCAAA GGAGCTATCT CCCAGCAGCA GTTGCAGCAA GAAGCCAACG
    AAGCCTAGTC AAGATGTGCC GTCAATCAGT CTGTCCATTT CCACCACGCC AAGGGCGACG
    ACCAGCACCA GCACTACTAC TACACAAAAG ACCACGGCTG CCACGCAGAC CGAGGGAGCC
    AAAAAGCTAA TCGGCGGGAT CCACAAGCTG CCCAGTAGCA GTTGTATTGC GAAGGGAGCC
    GGAGCCGTTT TGGAGGCCAC CGCCGGCAGG CGTGCTGTTA TCATACTCAA CGATGATCGC
    GAAGCAGGCA GGAGCAACAA TGAGTTCATA TTCGGTGATT TCAACGAGGA TGAGCTAAAA
    CTCTTCGACG ACAACTTGGA TGATGAGGAG GAGGGCCAGC ACAAGCAGTC TTCAAACAAG
    AAACAGCAAA CTGAAGGGGA ATCTGATGTG ACCCAAGATG ACACCGAAGA CGTGGACCAA
    GATCTGGACA AGGAACTGGA ATGCCTGGGA CGACGACCAG AGAAGAAGCA AGAGCGACAG
    CAGGATATCA GCGCCAGCAG CGATCAGCAT CTGAATGATT CTGGTGCGGC TTCGGATGCA
    GTCAACAGCT CAGCCAGCCT TGACATGCTC TCAATTTCTG CAGAGGCGGC ACAATCGTCA
    CCTAATGCGG GGGCAACCGC TACCTCATCC AGCGTCGCCA GTCCCATTAA CTCGTCCACG
    CCCTCGGGTG CTTCGTCCGC ATCGGCGAGC ACGTCCACCT CATCGTCCTC GGTCTCGATT
    TCATCATCGT CGGGCGGCAA CGGTGCCGCT TGTCTTGCAT CCGTTTCCGG TATGCTTGGT
    GGAGTGGCCA ATCAGTCCGG GGACAGCGGC ATCTATGCTG CCGCCAACAC GTCTATTAAG
    GAGCACGTTA ACACTTTCCT AAGCAAGGCC AGCAGCAGCG AGGAGACGCT GCCGAGTCCC
    AACTCCATAT CGATGCAACA GCTGGAAACA TGCAACGACA TCGAGGCGGC CATCATAGCC
    GCAGCCCGTG CTGCCGCTGC TGCCCGGAGC ACCAGCTGCA GTCGCAGCAA CTCTCAGGAG
    CAGGATGCCT TGCAGCCGCA GGTCAAGACC AAGGCGACTC CCAATCCGGA GGCGAAGATG
    GTTCTGCCTG TGTTTGTGAC CTACAACAAC AACAACAACG ACGACGATGA CGACGAGGAG
    GACGACGAGA GCCTGCAAGA ACTGAGCTTC ATGGCCGACC TTAAGGCGGA TGCAGCCACG
    GAGGATATTG CAATGCAGCC GCCACCGCCT TCATGTCCGG CGATGATGAC CAACACGGAA
    CTAATCGTCG ACTACATCGC CAAAACCTGG AATGCCATTG CCAACAGTAA GTACGTAACG
    TACTACAACG AGCAAGAGCA GGAGCAGGAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001284853.1
    CDS725..6460
    Translation

    Target ORF information:

    RefSeq Version NM_001297924.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twenty-four (tyf), transcript variant N, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001297924.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGAACAATG AACAAACGCC GTCATCATCG GACACACCGG CGGATCGTGT GGCATCTGGT 
    GGAGCAGCTG CCAAACTGCC AACGATCATA CCGGACACGC CCATCGTCTC GATTGCCGCT
    GGCACGATGA GCGAGAGCAC CACGACGACC ACCGTCACCA AAACAACGCC GACGCCGTTC
    CCACAGGAGC AGCTTATGAC CATGATGTCA GACCATGTGG CGAAGGCAGA CGCGCAGCTT
    GACGAGGCCT GTAGGAACCA GGGGGCCAAA GACGAGGACA GCCAAGGAGC GGCACCAGTG
    GCTGCCAACC ATGTAGCCAC CAGCGGCAGT AGCAGCAGAG GCAATGACCC CAAGAAGTCA
    TCATCCCCTA GTAATAGCCA TAGCTCTGGT AACCACGCAG CCACGGCAGA CGCTGCCAAA
    GGATCCGCAG CAGAAGCAGC AGCAGGAGAT ACGTCCTTCT CGCAGGTCTT TGCCATGCTA
    AAGGATTCGC CCAGCGCTGA ACTGTCGCGA CCAAACCGCA GGGTACTCCA GTACGGAGCC
    ACTGCCAAGG GCAAGACCCA GGACGATCAG AATGTCGGCA AGCGACAGAA GAAGAAAAAG
    ACAAAATACG ATACCTGGGA AGATAGCGAT TTTAATGATC TGGACGACGC ATCATTGAAG
    AAACTTCTCG AAGAGGCCTA CTGGTATCGA AATCCTGGCG ACAGGAAGAA CAAGAGCGAG
    CGATTTCTGC AAATGCTCAA AAAGGCTGAG TACGACGAAG AGATCTCTTA TCGTGCCATC
    AAATCCTGCC TCACAATCAA CCCATCCGCA CTAACGTCCA GTGCAGCAAC AGGCTCGAGC
    GGCGGGAGCA GCAGCAGTGC AGGGCACAAC AGCAGCTATA ATTCCACCAA TCACCGGTCA
    ACGGATCCCG GCCAGCGGCA CAAGCAGGGC GGCTCCCTGC AGGATCTCGT TGAGGCGGCG
    CACCGCAGCG AACTGGCCAC AACCTCGGCG GCGGCGGCAG CAGCAGCGAC AGCGGCAGCA
    ACCCAACGTT TCAACTGTGA TTACCAGCTG AGTGGTCGCT CCAATCGCCG GCAGCAGCAG
    CAGCAACAGC AGCACAACCA AAACGCCACC TCCTCCGCAT CCGCATCCAA AAAGATCAAG
    AACTTAAACG TCTCGGGCCG GCAGCGCGAA GGAGGCAGCC TGCCCAGCAG TGTTAACTTT
    GAGCCGGCAT CGTCTGCCAT GGAAGCCAAG CAGAATAAGC AACAGGCTCA GCCTTCGACA
    GAAGCTGCAG GTGGCTCGTT TTCGGGGTCG GTCGGTAACA AGCACCACCA CCGCAGCAGC
    ACCGGCAGTT GCAGCAGCCT GATAACTCAA TCGCGCGATA CGGAGGCAAA CAATCAGTTC
    GACTTGGACA TGGACGATGC GACGGGTGTT GGTGCCAGCG CCGTTGTCGG TGCCAGCGGC
    GACCAAGACG ACAACATGTT GGAGCAATTG GATCCTCAGA ACCCCTCGAT GAAGTTTCTG
    ATTGACGCCC TAAACGACAC GCCATTGGAG GCCAGGTACA CCGGTGCCGG CAAAAAGTTC
    CTAATCGACG ACCGGTTGCA TTTTTCCGTC CTGGAGCTGT CTGCCAGGAG GAAGCTGCAG
    CAGCAGGCAC AATACCTGGC CGCCCTCACC GGACAATTTG GTCTCGGCCT GGAGGAGGAG
    AAGCGCAAGG TAGAGGCCGG CTACGTGTCG CTCAACGACA ACTACACGGC GTGCTCCTCA
    GTTTACGGCG GCGGTGCAGG TTCGCCAGCT GCCGTGGAGG GCAGCAAGCC CTCTACCTCC
    TCCTCGGCCG TGTCTGGCGG TAGCAACTGC GGTGCCTCCT CTGACGCACT GTCTAGCGAT
    CTGCGACCTG CGAAGATTGG ACGCATGGTC CCCGCCGCAG CGGCAGTGGC AGCTGCGCCA
    ATGGGTGGCA AGACGACTAC TCGACAGCTA GACGAGAATG GAAATGCATT GGGCGATGGT
    TTCGGGGGCA GTGCAGCCAG TGGCTCCAGT GGCGCCAGTG GCGCCAGTGC TGCCGCCAGC
    AGTAGCAACG GCAACGGTAA CGCAAACCAG TTCACAGCTC TAATAACCCC CACGGTGGGA
    TCCAGTGTAC CCACCTCTAC TGTCGCTCAC CGTCAGAATA GTGTCTCCAC GCTGCTCTCC
    ACGGGCACCA ACACTACAAC AACGGCCATG GCAGCGGCGG CGGTGGCTGC ATCCTACAGC
    CAGTTGCAAC AGGCACCGGT CGGAGGCGCG GCTGGAACGG TTTCTGGGAT CGGCATTGGC
    ATGCAGCAGC CCACCAAGCA GAAGACGAAG AAGAAGTCGC AGCAGGAGCG CAACACCACC
    ACCGTCGATG TGGAGTCGGT TGCCGGCTAT AGGGGCAATG ATCCTGTTGA GCAGCTCGTC
    AAGTACATTG AAAACGATGT CAACGGTGGC GGAAACAGTG CCGCTGGACA GCGCAAGAAG
    GAGCGCAAGA AGCAAAACAA GCTCAAGAAG AGCAACTCGT TGGAGGAGCT GCGCACCTGC
    TCAAAAATGG AGGTGGACGA TTTGAAGCGC CAGTCGGCGA CCACGGAGAT GATGCGACAT
    AAGAAGGGCA CGAACAATGC ATCCTCGGGC GGATCGTCGT CGACGGCCTC TGGTTCCTCA
    AACAGTGGCA AGCACAACTC GGCATCCGTG GCGGATATTA ACAAAAACTG CAACAAGGAG
    CAGCAACAGC AATCGACGCC GACGGTGCAA GTGCGCAACA GCAACAATCC AGGAACGCAG
    CAGCGGAAGG GCGAGCGTCG GTCCTGGGGC ACCGAGGAGT TGCAGTACCT GGGTGACCAC
    CAGGAAATAT CCGCTGCCTG GTCGGAGCTG GAGACTGTGG GCCAGAAGGA GCTGCCGCTG
    GCGCTGCCCG CCTTGGCGCG CATGTCCGAA CTGGATGCCC TGAATACCGT CTTGTCGGAG
    ACCGCTGAGT TCCATGTGGT TACCAAGAAG AAGAAACCAA AGAAGCAGCG TGCTGTTACC
    ATGGACGATG CGGCGGTGGC TGCAGCTGCC GTCACGGGCG GCAACTTACA GCGCATGCAG
    CAGATCACCA AGTCCGCGTC CTCCAACATC ATGTCCCAGC GGATGCACTA CTATACTCCG
    TACAACAGCA ACAATGGTGG AGGCAGTGTC AACTATAAGC AACAGCAGCA ACAGAGCCAA
    CAGTACCAGG AGCACTACCA GGCGCCACAG GGGCAGCACC ACCATCACCA CCATCATCAT
    CACCACCATC ATCACCATCA CAGCGGCTCG GCGGTTTCGA ATCAGGTGGA TAGCTCACGG
    CGCAAGTCCA CGTCGTCTAT GCCCCCATCG GAGAAATCTG ACTCCAGTGA TCTCGACTCG
    GTCCACTCCC TGCCCATCCA GACGGGGAAG AAGAAGAGCC TAGGCGGTGG CAACAACAAA
    CAACGGGGGG CGCCGTCAGC CAGTCAACGC CAGGCCAAGC AAAACAACAA TGCTGCAGCC
    CCCATTTCGT ATGCGGATAT CGCCCGGAAC AAGCAGGAGG CTCTAAACAA TGCCACGGCC
    AGCGACACCG AGTTGGAGCT GGGCGACAAG ATGCAGATCA AGAGTGGCGG CAAATCCAAG
    TCGCGACCCG ATTTCCCGGA GCTGCCAGGT GCTGTTACGG CAGTGGCTGC CAGTGGAGGT
    GCAGCCAATC AGGCGACGGT TGTCAGCAGC CAAAATACGC CACCATCCTC GTCCAGTTCC
    ATCAGTTATT CGCAGAGTCT CAATGCAACG CCGCCCAGCA GCAGCAGCGA TGCGGAGTCC
    ACCAACTCGC CGGAGCTGCT GGCGCAGGTG CAGATACCGG CTTACAGCAT GATCACGCCA
    ACTCTAACAG CCTCCATGAT CAGCAGTGTC AGCAGTTCTG TCGCCAGCAC CTCTTCCACA
    TCCTGCTCAC CGCCAGCTCT GCAGAAATCC AAGAGCGTGG AGCACGACAC CAGCTACAGC
    TGCAATAGTA GCAACTTGGA TCAACAGTAT CCGGCACTAG AAAAGACCGT CAAGCGGCAT
    AGCACCAACA ACGTGTCTGT GGCCGTCGCA GCCTCCACCT CGGCATCGGC GTCCTCCTCG
    TTGTACAACT TTGCAGCGGC AGCCAAGCAG CAGCTGACAG AGAACACCCA TTCGGCCGTA
    TCGCCGCCGG CAGCTGTAAC AACTACCTCA ACCGCAGTCG CAGCCTCAGC TCCAGCTCCA
    ACTACAGTTA CAGTTCCAGC TGCAGTTACA GTTCCTTGTT ATTCCTCATC CTCCACACAC
    GGCTCGACTC AAACTTCAAC TTCTACTGCA TCGTCTGCTA TAGCAGCTTT ACCCTCGGGC
    AAGGCCAAGG CCAAGCCAAA GGAGCTATCT CCCAGCAGCA GTTGCAGCAA GAAGCCAACG
    AAGCCTAGTC AAGATGTGCC GTCAATCAGT CTGTCCATTT CCACCACGCC AAGGGCGACG
    ACCAGCACCA GCACTACTAC TACACAAAAG ACCACGGCTG CCACGCAGAC CGAGGGAGCC
    AAAAAGCTAA TCGGCGGGAT CCACAAGCTG CCCAGTAGCA GTTGTATTGC GAAGGGAGCC
    GGAGCCGTTT TGGAGGCCAC CGCCGGCAGG CGTGCTGTTA TCATACTCAA CGATGATCGC
    GAAGCAGGCA GGAGCAACAA TGAGTTCATA TTCGGTGATT TCAACGAGGA TGAGCTAAAA
    CTCTTCGACG ACAACTTGGA TGATGAGGAG GAGGGCCAGC ACAAGCAGTC TTCAAACAAG
    AAACAGCAAA CTGAAGGGGA ATCTGATGTG ACCCAAGATG ACACCGAAGA CGTGGACCAA
    GATCTGGACA AGGAACTGGA ATGCCTGGGA CGACGACCAG AGAAGAAGCA AGAGCGACAG
    CAGGATATCA GCGCCAGCAG CGATCAGCAT CTGAATGATT CTGGTGCGGC TTCGGATGCA
    GTCAACAGCT CAGCCAGCCT TGACATGCTC TCAATTTCTG CAGAGGCGGC ACAATCGTCA
    CCTAATGCGG GGGCAACCGC TACCTCATCC AGCGTCGCCA GTCCCATTAA CTCGTCCACG
    CCCTCGGGTG CTTCGTCCGC ATCGGCGAGC ACGTCCACCT CATCGTCCTC GGTCTCGATT
    TCATCATCGT CGGGCGGCAA CGGTGCCGCT TGTCTTGCAT CCGTTTCCGG TATGCTTGGT
    GGAGTGGCCA ATCAGTCCGG GGACAGCGGC ATCTATGCTG CCGCCAACAC GTCTATTAAG
    GAGCACGTTA ACACTTTCCT AAGCAAGGCC AGCAGCAGCG AGGAGACGCT GCCGAGTCCC
    AACTCCATAT CGATGCAACA GCTGGAAACA TGCAACGACA TCGAGGCGGC CATCATAGCC
    GCAGCCCGTG CTGCCGCTGC TGCCCGGAGC ACCAGCTGCA GTCGCAGCAA CTCTCAGGAG
    CAGGATGCCT TGCAGCCGCA GGTCAAGACC AAGGCGACTC CCAATCCGGA GGCGAAGATG
    GTTCTGCCTG TGTTTGTGAC CTACAACAAC AACAACAACG ACGACGATGA CGACGAGGAG
    GACGACGAGA GCCTGCAAGA ACTGAGCTTC ATGGCCGACC TTAAGGCGGA TGCAGCCACG
    GAGGATATTG CAATGCAGCC GCCACCGCCT TCATGTCCGG CGATGATGAC CAACACGGAA
    CTAATCGTCG ACTACATCGC CAAAACCTGG AATGCCATTG CCAACAGTAA GTACGTAACG
    TACTACAACG AGCAAGAGCA GGAGCAGGAG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03750
    Clone ID Related Accession (Same CDS sequence) NM_001258589.2 , NM_001169189.3 , NM_001258591.2 , NM_001258588.2 , NM_001169188.3 , NM_001258592.2
    Accession Version NM_001258589.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twenty-four, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_001258589.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTCAAAA AGGCTGAGTA CGACGAAGAG ATCTCTTATC GTGCCATCAA ATCCTGCCTC 
    ACAATCAACC CATCCGCACT AACGTCCAGT GCAGCAACAG GCTCGAGCGG CGGGAGCAGC
    AGCAGTGCAG GGCACAACAG CAGCTATAAT TCCACCAATC ACCGGTCAAC GGATCCCGGC
    CAGCGGCACA AGCAGGGCGG CTCCCTGCAG GATCTCGTTG AGGCGGCGCA CCGCAGCGAA
    CTGGCCACAA CCTCGGCGGC GGCGGCAGCA GCAGCGACAG CGGCAGCAAC CCAACGTTTC
    AACTGTGATT ACCAGCTGAG TGGTCGCTCC AATCGCCGGC AGCAGCAGCA GCAACAGCAG
    CACAACCAAA ACGCCACCTC CTCCGCATCC GCATCCAAAA AGATCAAGAA CTTAAACGTC
    TCGGGCCGGC AGCGCGAAGG AGGCAGCCTG CCCAGCAGTG TTAACTTTGA GCCGGCATCG
    TCTGCCATGG AAGCCAAGCA GAATAAGCAA CAGGCTCAGC CTTCGACAGA AGCTGCAGGT
    GGCTCGTTTT CGGGGTCGGT CGGTAACAAG CACCACCACC GCAGCAGCAC CGGCAGTTGC
    AGCAGCCTGA TAACTCAATC GCGCGATACG GAGGCAAACA ATCAGTTCGA CTTGGACATG
    GACGATGCGA CGGGTGTTGG TGCCAGCGCC GTTGTCGGTG CCAGCGGCGA CCAAGACGAC
    AACATGTTGG AGCAATTGGA TCCTCAGAAC CCCTCGATGA AGTTTCTGAT TGACGCCCTA
    AACGACACGC CATTGGAGGC CAGGTACACC GGTGCCGGCA AAAAGTTCCT AATCGACGAC
    CGGTTGCATT TTTCCGTCCT GGAGCTGTCT GCCAGGAGGA AGCTGCAGCA GCAGGCACAA
    TACCTGGCCG CCCTCACCGG ACAATTTGGT CTCGGCCTGG AGGAGGAGAA GCGCAAGGTA
    GAGGCCGGCT ACGTGTCGCT CAACGACAAC TACACGGCGT GCTCCTCAGT TTACGGCGGC
    GGTGCAGGTT CGCCAGCTGC CGTGGAGGGC AGCAAGCCCT CTACCTCCTC CTCGGCCGTG
    TCTGGCGGTA GCAACTGCGG TGCCTCCTCT GACGCACTGT CTAGCGATCT GCGACCTGCG
    AAGATTGGAC GCATGGTCCC CGCCGCAGCG GCAGTGGCAG CTGCGCCAAT GGGTGGCAAG
    ACGACTACTC GACAGCTAGA CGAGAATGGA AATGCATTGG GCGATGGTTT CGGGGGCAGT
    GCAGCCAGTG GCTCCAGTGG CGCCAGTGGC GCCAGTGCTG CCGCCAGCAG TAGCAACGGC
    AACGGTAACG CAAACCAGTT CACAGCTCTA ATAACCCCCA CGGTGGGATC CAGTGTACCC
    ACCTCTACTG TCGCTCACCG TCAGAATAGT GTCTCCACGC TGCTCTCCAC GGGCACCAAC
    ACTACAACAA CGGCCATGGC AGCGGCGGCG GTGGCTGCAT CCTACAGCCA GTTGCAACAG
    GCACCGGTCG GAGGCGCGGC TGGAACGGTT TCTGGGATCG GCATTGGCAT GCAGCAGCCC
    ACCAAGCAGA AGACGAAGAA GAAGTCGCAG CAGGAGCGCA ACACCACCAC CGTCGATGTG
    GAGTCGGTTG CCGGCTATAG GGGCAATGAT CCTGTTGAGC AGCTCGTCAA GTACATTGAA
    AACGATGTCA ACGGTGGCGG AAACAGTGCC GCTGGACAGC GCAAGAAGGA GCGCAAGAAG
    CAAAACAAGC TCAAGAAGAG CAACTCGTTG GAGGAGCTGC GCACCTGCTC AAAAATGGAG
    GTGGACGATT TGAAGCGCCA GTCGGCGACC ACGGAGATGA TGCGACATAA GAAGGGCACG
    AACAATGCAT CCTCGGGCGG ATCGTCGTCG ACGGCCTCTG GTTCCTCAAA CAGTGGCAAG
    CACAACTCGG CATCCGTGGC GGATATTAAC AAAAACTGCA ACAAGGAGCA GCAACAGCAA
    TCGACGCCGA CGGTGCAAGT GCGCAACAGC AACAATCCAG GAACGCAGCA GCGGAAGGGC
    GAGCGTCGGT CCTGGGGCAC CGAGGAGTTG CAGTACCTGG GTGACCACCA GGAAATATCC
    GCTGCCTGGT CGGAGCTGGA GACTGTGGGC CAGAAGGAGC TGCCGCTGGC GCTGCCCGCC
    TTGGCGCGCA TGTCCGAACT GGATGCCCTG AATACCGTCT TGTCGGAGAC CGCTGAGTTC
    CATGTGGTTA CCAAGAAGAA GAAACCAAAG AAGCAGCGTG CTGTTACCAT GGACGATGCG
    GCGGTGGCTG CAGCTGCCGT CACGGGCGGC AACTTACAGC GCATGCAGCA GATCACCAAG
    TCCGCGTCCT CCAACATCAT GTCCCAGCGG ATGCACTACT ATACTCCGTA CAACAGCAAC
    AATGGTGGAG GCAGTGTCAA CTATAAGCAA CAGCAGCAAC AGAGCCAACA GTACCAGGAG
    CACTACCAGG CGCCACAGGG GCAGCACCAC CATCACCACC ATCATCATCA CCACCATCAT
    CACCATCACA GCGGCTCGGC GGTTTCGAAT CAGGTGGATA GCTCACGGCG CAAGTCCACG
    TCGTCTATGC CCCCATCGGA GAAATCTGAC TCCAGTGATC TCGACTCGGT CCACTCCCTG
    CCCATCCAGA CGGGGAAGAA GAAGAGCCTA GGCGGTGGCA ACAACAAACA ACGGGGGGCG
    CCGTCAGCCA GTCAACGCCA GGCCAAGCAA AACAACAATG CTGCAGCCCC CATTTCGTAT
    GCGGATATCG CCCGGAACAA GCAGGAGGCT CTAAACAATG CCACGGCCAG CGACACCGAG
    TTGGAGCTGG GCGACAAGAT GCAGATCAAG AGTGGCGGCA AATCCAAGTC GCGACCCGAT
    TTCCCGGAGC TGCCAGGTGC TGTTACGGCA GTGGCTGCCA GTGGAGGTGC AGCCAATCAG
    GCGACGGTTG TCAGCAGCCA AAATACGCCA CCATCCTCGT CCAGTTCCAT CAGTTATTCG
    CAGAGTCTCA ATGCAACGCC GCCCAGCAGC AGCAGCGATG CGGAGTCCAC CAACTCGCCG
    GAGCTGCTGG CGCAGGTGCA GATACCGGCT TACAGCATGA TCACGCCAAC TCTAACAGCC
    TCCATGATCA GCAGTGTCAG CAGTTCTGTC GCCAGCACCT CTTCCACATC CTGCTCACCG
    CCAGCTCTGC AGAAATCCAA GAGCGTGGAG CACGACACCA GCTACAGCTG CAATAGTAGC
    AACTTGGATC AACAGTATCC GGCACTAGAA AAGACCGTCA AGCGGCATAG CACCAACAAC
    GTGTCTGTGG CCGTCGCAGC CTCCACCTCG GCATCGGCGT CCTCCTCGTT GTACAACTTT
    GCAGCGGCAG CCAAGCAGCA GCTGACAGAG AACACCCATT CGGCCGTATC GCCGCCGGCA
    GCTGTAACAA CTACCTCAAC CGCAGTCGCA GCCTCAGCTC CAGCTCCAAC TACAGTTACA
    GTTCCAGCTG CAGTTACAGT TCCTTGTTAT TCCTCATCCT CCACACACGG CTCGACTCAA
    ACTTCAACTT CTACTGCATC GTCTGCTATA GCAGCTTTAC CCTCGGGCAA GGCCAAGGCC
    AAGCCAAAGG AGCTATCTCC CAGCAGCAGT TGCAGCAAGA AGCCAACGAA GCCTAGTCAA
    GATGTGCCGT CAATCAGTCT GTCCATTTCC ACCACGCCAA GGGCGACGAC CAGCACCAGC
    ACTACTACTA CACAAAAGAC CACGGCTGCC ACGCAGACCG AGGGAGCCAA AAAGCTAATC
    GGCGGGATCC ACAAGCTGCC CAGTAGCAGT TGTATTGCGA AGGGAGCCGG AGCCGTTTTG
    GAGGCCACCG CCGGCAGGCG TGCTGTTATC ATACTCAACG ATGATCGCGA AGCAGGCAGG
    AGCAACAATG AGTTCATATT CGGTGATTTC AACGAGGATG AGCTAAAACT CTTCGACGAC
    AACTTGGATG ATGAGGAGGA GGGCCAGCAC AAGCAGTCTT CAAACAAGAA ACAGCAAACT
    GAAGGGGAAT CTGATGTGAC CCAAGATGAC ACCGAAGACG TGGACCAAGA TCTGGACAAG
    GAACTGGAAT GCCTGGGACG ACGACCAGAG AAGAAGCAAG AGCGACAGCA GGATATCAGC
    GCCAGCAGCG ATCAGCATCT GAATGATTCT GGTGCGGCTT CGGATGCAGT CAACAGCTCA
    GCCAGCCTTG ACATGCTCTC AATTTCTGCA GAGGCGGCAC AATCGTCACC TAATGCGGGG
    GCAACCGCTA CCTCATCCAG CGTCGCCAGT CCCATTAACT CGTCCACGCC CTCGGGTGCT
    TCGTCCGCAT CGGCGAGCAC GTCCACCTCA TCGTCCTCGG TCTCGATTTC ATCATCGTCG
    GGCGGCAACG GTGCCGCTTG TCTTGCATCC GTTTCCGGTA TGCTTGGTGG AGTGGCCAAT
    CAGTCCGGGG ACAGCGGCAT CTATGCTGCC GCCAACACGT CTATTAAGGA GCACGTTAAC
    ACTTTCCTAA GCAAGGCCAG CAGCAGCGAG GAGACGCTGC CGAGTCCCAA CTCCATATCG
    ATGCAACAGC TGGAAACATG CAACGACATC GAGGCGGCCA TCATAGCCGC AGCCCGTGCT
    GCCGCTGCTG CCCGGAGCAC CAGCTGCAGT CGCAGCAACT CTCAGGAGCA GGATGCCTTG
    CAGCCGCAGG TCAAGACCAA GGCGACTCCC AATCCGGAGG CGAAGATGGT TCTGCCTGTG
    TTTGTGACCT ACAACAACAA CAACAACGAC GACGATGACG ACGAGGAGGA CGACGAGAGC
    CTGCAAGAAC TGAGCTTCAT GGCCGACCTT AAGGCGGATG CAGCCACGGA GGATATTGCA
    ATGCAGCCGC CACCGCCTTC ATGTCCGGCG ATGATGACCA ACACGGAACT AATCGTCGAC
    TACATCGCCA AAACCTGGAA TGCCATTGCC AACAGTAAGT ACGTAACGTA CTACAACGAG
    CAAGAGCAGG AGCAGGAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245518.1
    CDS190..5193
    Translation

    Target ORF information:

    RefSeq Version NM_001258589.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twenty-four (tyf), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258589.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTCAAAA AGGCTGAGTA CGACGAAGAG ATCTCTTATC GTGCCATCAA ATCCTGCCTC 
    ACAATCAACC CATCCGCACT AACGTCCAGT GCAGCAACAG GCTCGAGCGG CGGGAGCAGC
    AGCAGTGCAG GGCACAACAG CAGCTATAAT TCCACCAATC ACCGGTCAAC GGATCCCGGC
    CAGCGGCACA AGCAGGGCGG CTCCCTGCAG GATCTCGTTG AGGCGGCGCA CCGCAGCGAA
    CTGGCCACAA CCTCGGCGGC GGCGGCAGCA GCAGCGACAG CGGCAGCAAC CCAACGTTTC
    AACTGTGATT ACCAGCTGAG TGGTCGCTCC AATCGCCGGC AGCAGCAGCA GCAACAGCAG
    CACAACCAAA ACGCCACCTC CTCCGCATCC GCATCCAAAA AGATCAAGAA CTTAAACGTC
    TCGGGCCGGC AGCGCGAAGG AGGCAGCCTG CCCAGCAGTG TTAACTTTGA GCCGGCATCG
    TCTGCCATGG AAGCCAAGCA GAATAAGCAA CAGGCTCAGC CTTCGACAGA AGCTGCAGGT
    GGCTCGTTTT CGGGGTCGGT CGGTAACAAG CACCACCACC GCAGCAGCAC CGGCAGTTGC
    AGCAGCCTGA TAACTCAATC GCGCGATACG GAGGCAAACA ATCAGTTCGA CTTGGACATG
    GACGATGCGA CGGGTGTTGG TGCCAGCGCC GTTGTCGGTG CCAGCGGCGA CCAAGACGAC
    AACATGTTGG AGCAATTGGA TCCTCAGAAC CCCTCGATGA AGTTTCTGAT TGACGCCCTA
    AACGACACGC CATTGGAGGC CAGGTACACC GGTGCCGGCA AAAAGTTCCT AATCGACGAC
    CGGTTGCATT TTTCCGTCCT GGAGCTGTCT GCCAGGAGGA AGCTGCAGCA GCAGGCACAA
    TACCTGGCCG CCCTCACCGG ACAATTTGGT CTCGGCCTGG AGGAGGAGAA GCGCAAGGTA
    GAGGCCGGCT ACGTGTCGCT CAACGACAAC TACACGGCGT GCTCCTCAGT TTACGGCGGC
    GGTGCAGGTT CGCCAGCTGC CGTGGAGGGC AGCAAGCCCT CTACCTCCTC CTCGGCCGTG
    TCTGGCGGTA GCAACTGCGG TGCCTCCTCT GACGCACTGT CTAGCGATCT GCGACCTGCG
    AAGATTGGAC GCATGGTCCC CGCCGCAGCG GCAGTGGCAG CTGCGCCAAT GGGTGGCAAG
    ACGACTACTC GACAGCTAGA CGAGAATGGA AATGCATTGG GCGATGGTTT CGGGGGCAGT
    GCAGCCAGTG GCTCCAGTGG CGCCAGTGGC GCCAGTGCTG CCGCCAGCAG TAGCAACGGC
    AACGGTAACG CAAACCAGTT CACAGCTCTA ATAACCCCCA CGGTGGGATC CAGTGTACCC
    ACCTCTACTG TCGCTCACCG TCAGAATAGT GTCTCCACGC TGCTCTCCAC GGGCACCAAC
    ACTACAACAA CGGCCATGGC AGCGGCGGCG GTGGCTGCAT CCTACAGCCA GTTGCAACAG
    GCACCGGTCG GAGGCGCGGC TGGAACGGTT TCTGGGATCG GCATTGGCAT GCAGCAGCCC
    ACCAAGCAGA AGACGAAGAA GAAGTCGCAG CAGGAGCGCA ACACCACCAC CGTCGATGTG
    GAGTCGGTTG CCGGCTATAG GGGCAATGAT CCTGTTGAGC AGCTCGTCAA GTACATTGAA
    AACGATGTCA ACGGTGGCGG AAACAGTGCC GCTGGACAGC GCAAGAAGGA GCGCAAGAAG
    CAAAACAAGC TCAAGAAGAG CAACTCGTTG GAGGAGCTGC GCACCTGCTC AAAAATGGAG
    GTGGACGATT TGAAGCGCCA GTCGGCGACC ACGGAGATGA TGCGACATAA GAAGGGCACG
    AACAATGCAT CCTCGGGCGG ATCGTCGTCG ACGGCCTCTG GTTCCTCAAA CAGTGGCAAG
    CACAACTCGG CATCCGTGGC GGATATTAAC AAAAACTGCA ACAAGGAGCA GCAACAGCAA
    TCGACGCCGA CGGTGCAAGT GCGCAACAGC AACAATCCAG GAACGCAGCA GCGGAAGGGC
    GAGCGTCGGT CCTGGGGCAC CGAGGAGTTG CAGTACCTGG GTGACCACCA GGAAATATCC
    GCTGCCTGGT CGGAGCTGGA GACTGTGGGC CAGAAGGAGC TGCCGCTGGC GCTGCCCGCC
    TTGGCGCGCA TGTCCGAACT GGATGCCCTG AATACCGTCT TGTCGGAGAC CGCTGAGTTC
    CATGTGGTTA CCAAGAAGAA GAAACCAAAG AAGCAGCGTG CTGTTACCAT GGACGATGCG
    GCGGTGGCTG CAGCTGCCGT CACGGGCGGC AACTTACAGC GCATGCAGCA GATCACCAAG
    TCCGCGTCCT CCAACATCAT GTCCCAGCGG ATGCACTACT ATACTCCGTA CAACAGCAAC
    AATGGTGGAG GCAGTGTCAA CTATAAGCAA CAGCAGCAAC AGAGCCAACA GTACCAGGAG
    CACTACCAGG CGCCACAGGG GCAGCACCAC CATCACCACC ATCATCATCA CCACCATCAT
    CACCATCACA GCGGCTCGGC GGTTTCGAAT CAGGTGGATA GCTCACGGCG CAAGTCCACG
    TCGTCTATGC CCCCATCGGA GAAATCTGAC TCCAGTGATC TCGACTCGGT CCACTCCCTG
    CCCATCCAGA CGGGGAAGAA GAAGAGCCTA GGCGGTGGCA ACAACAAACA ACGGGGGGCG
    CCGTCAGCCA GTCAACGCCA GGCCAAGCAA AACAACAATG CTGCAGCCCC CATTTCGTAT
    GCGGATATCG CCCGGAACAA GCAGGAGGCT CTAAACAATG CCACGGCCAG CGACACCGAG
    TTGGAGCTGG GCGACAAGAT GCAGATCAAG AGTGGCGGCA AATCCAAGTC GCGACCCGAT
    TTCCCGGAGC TGCCAGGTGC TGTTACGGCA GTGGCTGCCA GTGGAGGTGC AGCCAATCAG
    GCGACGGTTG TCAGCAGCCA AAATACGCCA CCATCCTCGT CCAGTTCCAT CAGTTATTCG
    CAGAGTCTCA ATGCAACGCC GCCCAGCAGC AGCAGCGATG CGGAGTCCAC CAACTCGCCG
    GAGCTGCTGG CGCAGGTGCA GATACCGGCT TACAGCATGA TCACGCCAAC TCTAACAGCC
    TCCATGATCA GCAGTGTCAG CAGTTCTGTC GCCAGCACCT CTTCCACATC CTGCTCACCG
    CCAGCTCTGC AGAAATCCAA GAGCGTGGAG CACGACACCA GCTACAGCTG CAATAGTAGC
    AACTTGGATC AACAGTATCC GGCACTAGAA AAGACCGTCA AGCGGCATAG CACCAACAAC
    GTGTCTGTGG CCGTCGCAGC CTCCACCTCG GCATCGGCGT CCTCCTCGTT GTACAACTTT
    GCAGCGGCAG CCAAGCAGCA GCTGACAGAG AACACCCATT CGGCCGTATC GCCGCCGGCA
    GCTGTAACAA CTACCTCAAC CGCAGTCGCA GCCTCAGCTC CAGCTCCAAC TACAGTTACA
    GTTCCAGCTG CAGTTACAGT TCCTTGTTAT TCCTCATCCT CCACACACGG CTCGACTCAA
    ACTTCAACTT CTACTGCATC GTCTGCTATA GCAGCTTTAC CCTCGGGCAA GGCCAAGGCC
    AAGCCAAAGG AGCTATCTCC CAGCAGCAGT TGCAGCAAGA AGCCAACGAA GCCTAGTCAA
    GATGTGCCGT CAATCAGTCT GTCCATTTCC ACCACGCCAA GGGCGACGAC CAGCACCAGC
    ACTACTACTA CACAAAAGAC CACGGCTGCC ACGCAGACCG AGGGAGCCAA AAAGCTAATC
    GGCGGGATCC ACAAGCTGCC CAGTAGCAGT TGTATTGCGA AGGGAGCCGG AGCCGTTTTG
    GAGGCCACCG CCGGCAGGCG TGCTGTTATC ATACTCAACG ATGATCGCGA AGCAGGCAGG
    AGCAACAATG AGTTCATATT CGGTGATTTC AACGAGGATG AGCTAAAACT CTTCGACGAC
    AACTTGGATG ATGAGGAGGA GGGCCAGCAC AAGCAGTCTT CAAACAAGAA ACAGCAAACT
    GAAGGGGAAT CTGATGTGAC CCAAGATGAC ACCGAAGACG TGGACCAAGA TCTGGACAAG
    GAACTGGAAT GCCTGGGACG ACGACCAGAG AAGAAGCAAG AGCGACAGCA GGATATCAGC
    GCCAGCAGCG ATCAGCATCT GAATGATTCT GGTGCGGCTT CGGATGCAGT CAACAGCTCA
    GCCAGCCTTG ACATGCTCTC AATTTCTGCA GAGGCGGCAC AATCGTCACC TAATGCGGGG
    GCAACCGCTA CCTCATCCAG CGTCGCCAGT CCCATTAACT CGTCCACGCC CTCGGGTGCT
    TCGTCCGCAT CGGCGAGCAC GTCCACCTCA TCGTCCTCGG TCTCGATTTC ATCATCGTCG
    GGCGGCAACG GTGCCGCTTG TCTTGCATCC GTTTCCGGTA TGCTTGGTGG AGTGGCCAAT
    CAGTCCGGGG ACAGCGGCAT CTATGCTGCC GCCAACACGT CTATTAAGGA GCACGTTAAC
    ACTTTCCTAA GCAAGGCCAG CAGCAGCGAG GAGACGCTGC CGAGTCCCAA CTCCATATCG
    ATGCAACAGC TGGAAACATG CAACGACATC GAGGCGGCCA TCATAGCCGC AGCCCGTGCT
    GCCGCTGCTG CCCGGAGCAC CAGCTGCAGT CGCAGCAACT CTCAGGAGCA GGATGCCTTG
    CAGCCGCAGG TCAAGACCAA GGCGACTCCC AATCCGGAGG CGAAGATGGT TCTGCCTGTG
    TTTGTGACCT ACAACAACAA CAACAACGAC GACGATGACG ACGAGGAGGA CGACGAGAGC
    CTGCAAGAAC TGAGCTTCAT GGCCGACCTT AAGGCGGATG CAGCCACGGA GGATATTGCA
    ATGCAGCCGC CACCGCCTTC ATGTCCGGCG ATGATGACCA ACACGGAACT AATCGTCGAC
    TACATCGCCA AAACCTGGAA TGCCATTGCC AACAGTAAGT ACGTAACGTA CTACAACGAG
    CAAGAGCAGG AGCAGGAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03750
    Clone ID Related Accession (Same CDS sequence) NM_001258589.2 , NM_001169189.3 , NM_001258591.2 , NM_001258588.2 , NM_001169188.3 , NM_001258592.2
    Accession Version NM_001169189.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twenty-four, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_001169189.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTCAAAA AGGCTGAGTA CGACGAAGAG ATCTCTTATC GTGCCATCAA ATCCTGCCTC 
    ACAATCAACC CATCCGCACT AACGTCCAGT GCAGCAACAG GCTCGAGCGG CGGGAGCAGC
    AGCAGTGCAG GGCACAACAG CAGCTATAAT TCCACCAATC ACCGGTCAAC GGATCCCGGC
    CAGCGGCACA AGCAGGGCGG CTCCCTGCAG GATCTCGTTG AGGCGGCGCA CCGCAGCGAA
    CTGGCCACAA CCTCGGCGGC GGCGGCAGCA GCAGCGACAG CGGCAGCAAC CCAACGTTTC
    AACTGTGATT ACCAGCTGAG TGGTCGCTCC AATCGCCGGC AGCAGCAGCA GCAACAGCAG
    CACAACCAAA ACGCCACCTC CTCCGCATCC GCATCCAAAA AGATCAAGAA CTTAAACGTC
    TCGGGCCGGC AGCGCGAAGG AGGCAGCCTG CCCAGCAGTG TTAACTTTGA GCCGGCATCG
    TCTGCCATGG AAGCCAAGCA GAATAAGCAA CAGGCTCAGC CTTCGACAGA AGCTGCAGGT
    GGCTCGTTTT CGGGGTCGGT CGGTAACAAG CACCACCACC GCAGCAGCAC CGGCAGTTGC
    AGCAGCCTGA TAACTCAATC GCGCGATACG GAGGCAAACA ATCAGTTCGA CTTGGACATG
    GACGATGCGA CGGGTGTTGG TGCCAGCGCC GTTGTCGGTG CCAGCGGCGA CCAAGACGAC
    AACATGTTGG AGCAATTGGA TCCTCAGAAC CCCTCGATGA AGTTTCTGAT TGACGCCCTA
    AACGACACGC CATTGGAGGC CAGGTACACC GGTGCCGGCA AAAAGTTCCT AATCGACGAC
    CGGTTGCATT TTTCCGTCCT GGAGCTGTCT GCCAGGAGGA AGCTGCAGCA GCAGGCACAA
    TACCTGGCCG CCCTCACCGG ACAATTTGGT CTCGGCCTGG AGGAGGAGAA GCGCAAGGTA
    GAGGCCGGCT ACGTGTCGCT CAACGACAAC TACACGGCGT GCTCCTCAGT TTACGGCGGC
    GGTGCAGGTT CGCCAGCTGC CGTGGAGGGC AGCAAGCCCT CTACCTCCTC CTCGGCCGTG
    TCTGGCGGTA GCAACTGCGG TGCCTCCTCT GACGCACTGT CTAGCGATCT GCGACCTGCG
    AAGATTGGAC GCATGGTCCC CGCCGCAGCG GCAGTGGCAG CTGCGCCAAT GGGTGGCAAG
    ACGACTACTC GACAGCTAGA CGAGAATGGA AATGCATTGG GCGATGGTTT CGGGGGCAGT
    GCAGCCAGTG GCTCCAGTGG CGCCAGTGGC GCCAGTGCTG CCGCCAGCAG TAGCAACGGC
    AACGGTAACG CAAACCAGTT CACAGCTCTA ATAACCCCCA CGGTGGGATC CAGTGTACCC
    ACCTCTACTG TCGCTCACCG TCAGAATAGT GTCTCCACGC TGCTCTCCAC GGGCACCAAC
    ACTACAACAA CGGCCATGGC AGCGGCGGCG GTGGCTGCAT CCTACAGCCA GTTGCAACAG
    GCACCGGTCG GAGGCGCGGC TGGAACGGTT TCTGGGATCG GCATTGGCAT GCAGCAGCCC
    ACCAAGCAGA AGACGAAGAA GAAGTCGCAG CAGGAGCGCA ACACCACCAC CGTCGATGTG
    GAGTCGGTTG CCGGCTATAG GGGCAATGAT CCTGTTGAGC AGCTCGTCAA GTACATTGAA
    AACGATGTCA ACGGTGGCGG AAACAGTGCC GCTGGACAGC GCAAGAAGGA GCGCAAGAAG
    CAAAACAAGC TCAAGAAGAG CAACTCGTTG GAGGAGCTGC GCACCTGCTC AAAAATGGAG
    GTGGACGATT TGAAGCGCCA GTCGGCGACC ACGGAGATGA TGCGACATAA GAAGGGCACG
    AACAATGCAT CCTCGGGCGG ATCGTCGTCG ACGGCCTCTG GTTCCTCAAA CAGTGGCAAG
    CACAACTCGG CATCCGTGGC GGATATTAAC AAAAACTGCA ACAAGGAGCA GCAACAGCAA
    TCGACGCCGA CGGTGCAAGT GCGCAACAGC AACAATCCAG GAACGCAGCA GCGGAAGGGC
    GAGCGTCGGT CCTGGGGCAC CGAGGAGTTG CAGTACCTGG GTGACCACCA GGAAATATCC
    GCTGCCTGGT CGGAGCTGGA GACTGTGGGC CAGAAGGAGC TGCCGCTGGC GCTGCCCGCC
    TTGGCGCGCA TGTCCGAACT GGATGCCCTG AATACCGTCT TGTCGGAGAC CGCTGAGTTC
    CATGTGGTTA CCAAGAAGAA GAAACCAAAG AAGCAGCGTG CTGTTACCAT GGACGATGCG
    GCGGTGGCTG CAGCTGCCGT CACGGGCGGC AACTTACAGC GCATGCAGCA GATCACCAAG
    TCCGCGTCCT CCAACATCAT GTCCCAGCGG ATGCACTACT ATACTCCGTA CAACAGCAAC
    AATGGTGGAG GCAGTGTCAA CTATAAGCAA CAGCAGCAAC AGAGCCAACA GTACCAGGAG
    CACTACCAGG CGCCACAGGG GCAGCACCAC CATCACCACC ATCATCATCA CCACCATCAT
    CACCATCACA GCGGCTCGGC GGTTTCGAAT CAGGTGGATA GCTCACGGCG CAAGTCCACG
    TCGTCTATGC CCCCATCGGA GAAATCTGAC TCCAGTGATC TCGACTCGGT CCACTCCCTG
    CCCATCCAGA CGGGGAAGAA GAAGAGCCTA GGCGGTGGCA ACAACAAACA ACGGGGGGCG
    CCGTCAGCCA GTCAACGCCA GGCCAAGCAA AACAACAATG CTGCAGCCCC CATTTCGTAT
    GCGGATATCG CCCGGAACAA GCAGGAGGCT CTAAACAATG CCACGGCCAG CGACACCGAG
    TTGGAGCTGG GCGACAAGAT GCAGATCAAG AGTGGCGGCA AATCCAAGTC GCGACCCGAT
    TTCCCGGAGC TGCCAGGTGC TGTTACGGCA GTGGCTGCCA GTGGAGGTGC AGCCAATCAG
    GCGACGGTTG TCAGCAGCCA AAATACGCCA CCATCCTCGT CCAGTTCCAT CAGTTATTCG
    CAGAGTCTCA ATGCAACGCC GCCCAGCAGC AGCAGCGATG CGGAGTCCAC CAACTCGCCG
    GAGCTGCTGG CGCAGGTGCA GATACCGGCT TACAGCATGA TCACGCCAAC TCTAACAGCC
    TCCATGATCA GCAGTGTCAG CAGTTCTGTC GCCAGCACCT CTTCCACATC CTGCTCACCG
    CCAGCTCTGC AGAAATCCAA GAGCGTGGAG CACGACACCA GCTACAGCTG CAATAGTAGC
    AACTTGGATC AACAGTATCC GGCACTAGAA AAGACCGTCA AGCGGCATAG CACCAACAAC
    GTGTCTGTGG CCGTCGCAGC CTCCACCTCG GCATCGGCGT CCTCCTCGTT GTACAACTTT
    GCAGCGGCAG CCAAGCAGCA GCTGACAGAG AACACCCATT CGGCCGTATC GCCGCCGGCA
    GCTGTAACAA CTACCTCAAC CGCAGTCGCA GCCTCAGCTC CAGCTCCAAC TACAGTTACA
    GTTCCAGCTG CAGTTACAGT TCCTTGTTAT TCCTCATCCT CCACACACGG CTCGACTCAA
    ACTTCAACTT CTACTGCATC GTCTGCTATA GCAGCTTTAC CCTCGGGCAA GGCCAAGGCC
    AAGCCAAAGG AGCTATCTCC CAGCAGCAGT TGCAGCAAGA AGCCAACGAA GCCTAGTCAA
    GATGTGCCGT CAATCAGTCT GTCCATTTCC ACCACGCCAA GGGCGACGAC CAGCACCAGC
    ACTACTACTA CACAAAAGAC CACGGCTGCC ACGCAGACCG AGGGAGCCAA AAAGCTAATC
    GGCGGGATCC ACAAGCTGCC CAGTAGCAGT TGTATTGCGA AGGGAGCCGG AGCCGTTTTG
    GAGGCCACCG CCGGCAGGCG TGCTGTTATC ATACTCAACG ATGATCGCGA AGCAGGCAGG
    AGCAACAATG AGTTCATATT CGGTGATTTC AACGAGGATG AGCTAAAACT CTTCGACGAC
    AACTTGGATG ATGAGGAGGA GGGCCAGCAC AAGCAGTCTT CAAACAAGAA ACAGCAAACT
    GAAGGGGAAT CTGATGTGAC CCAAGATGAC ACCGAAGACG TGGACCAAGA TCTGGACAAG
    GAACTGGAAT GCCTGGGACG ACGACCAGAG AAGAAGCAAG AGCGACAGCA GGATATCAGC
    GCCAGCAGCG ATCAGCATCT GAATGATTCT GGTGCGGCTT CGGATGCAGT CAACAGCTCA
    GCCAGCCTTG ACATGCTCTC AATTTCTGCA GAGGCGGCAC AATCGTCACC TAATGCGGGG
    GCAACCGCTA CCTCATCCAG CGTCGCCAGT CCCATTAACT CGTCCACGCC CTCGGGTGCT
    TCGTCCGCAT CGGCGAGCAC GTCCACCTCA TCGTCCTCGG TCTCGATTTC ATCATCGTCG
    GGCGGCAACG GTGCCGCTTG TCTTGCATCC GTTTCCGGTA TGCTTGGTGG AGTGGCCAAT
    CAGTCCGGGG ACAGCGGCAT CTATGCTGCC GCCAACACGT CTATTAAGGA GCACGTTAAC
    ACTTTCCTAA GCAAGGCCAG CAGCAGCGAG GAGACGCTGC CGAGTCCCAA CTCCATATCG
    ATGCAACAGC TGGAAACATG CAACGACATC GAGGCGGCCA TCATAGCCGC AGCCCGTGCT
    GCCGCTGCTG CCCGGAGCAC CAGCTGCAGT CGCAGCAACT CTCAGGAGCA GGATGCCTTG
    CAGCCGCAGG TCAAGACCAA GGCGACTCCC AATCCGGAGG CGAAGATGGT TCTGCCTGTG
    TTTGTGACCT ACAACAACAA CAACAACGAC GACGATGACG ACGAGGAGGA CGACGAGAGC
    CTGCAAGAAC TGAGCTTCAT GGCCGACCTT AAGGCGGATG CAGCCACGGA GGATATTGCA
    ATGCAGCCGC CACCGCCTTC ATGTCCGGCG ATGATGACCA ACACGGAACT AATCGTCGAC
    TACATCGCCA AAACCTGGAA TGCCATTGCC AACAGTAAGT ACGTAACGTA CTACAACGAG
    CAAGAGCAGG AGCAGGAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162660.1
    CDS189..5192
    Translation

    Target ORF information:

    RefSeq Version NM_001169189.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twenty-four (tyf), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169189.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTCAAAA AGGCTGAGTA CGACGAAGAG ATCTCTTATC GTGCCATCAA ATCCTGCCTC 
    ACAATCAACC CATCCGCACT AACGTCCAGT GCAGCAACAG GCTCGAGCGG CGGGAGCAGC
    AGCAGTGCAG GGCACAACAG CAGCTATAAT TCCACCAATC ACCGGTCAAC GGATCCCGGC
    CAGCGGCACA AGCAGGGCGG CTCCCTGCAG GATCTCGTTG AGGCGGCGCA CCGCAGCGAA
    CTGGCCACAA CCTCGGCGGC GGCGGCAGCA GCAGCGACAG CGGCAGCAAC CCAACGTTTC
    AACTGTGATT ACCAGCTGAG TGGTCGCTCC AATCGCCGGC AGCAGCAGCA GCAACAGCAG
    CACAACCAAA ACGCCACCTC CTCCGCATCC GCATCCAAAA AGATCAAGAA CTTAAACGTC
    TCGGGCCGGC AGCGCGAAGG AGGCAGCCTG CCCAGCAGTG TTAACTTTGA GCCGGCATCG
    TCTGCCATGG AAGCCAAGCA GAATAAGCAA CAGGCTCAGC CTTCGACAGA AGCTGCAGGT
    GGCTCGTTTT CGGGGTCGGT CGGTAACAAG CACCACCACC GCAGCAGCAC CGGCAGTTGC
    AGCAGCCTGA TAACTCAATC GCGCGATACG GAGGCAAACA ATCAGTTCGA CTTGGACATG
    GACGATGCGA CGGGTGTTGG TGCCAGCGCC GTTGTCGGTG CCAGCGGCGA CCAAGACGAC
    AACATGTTGG AGCAATTGGA TCCTCAGAAC CCCTCGATGA AGTTTCTGAT TGACGCCCTA
    AACGACACGC CATTGGAGGC CAGGTACACC GGTGCCGGCA AAAAGTTCCT AATCGACGAC
    CGGTTGCATT TTTCCGTCCT GGAGCTGTCT GCCAGGAGGA AGCTGCAGCA GCAGGCACAA
    TACCTGGCCG CCCTCACCGG ACAATTTGGT CTCGGCCTGG AGGAGGAGAA GCGCAAGGTA
    GAGGCCGGCT ACGTGTCGCT CAACGACAAC TACACGGCGT GCTCCTCAGT TTACGGCGGC
    GGTGCAGGTT CGCCAGCTGC CGTGGAGGGC AGCAAGCCCT CTACCTCCTC CTCGGCCGTG
    TCTGGCGGTA GCAACTGCGG TGCCTCCTCT GACGCACTGT CTAGCGATCT GCGACCTGCG
    AAGATTGGAC GCATGGTCCC CGCCGCAGCG GCAGTGGCAG CTGCGCCAAT GGGTGGCAAG
    ACGACTACTC GACAGCTAGA CGAGAATGGA AATGCATTGG GCGATGGTTT CGGGGGCAGT
    GCAGCCAGTG GCTCCAGTGG CGCCAGTGGC GCCAGTGCTG CCGCCAGCAG TAGCAACGGC
    AACGGTAACG CAAACCAGTT CACAGCTCTA ATAACCCCCA CGGTGGGATC CAGTGTACCC
    ACCTCTACTG TCGCTCACCG TCAGAATAGT GTCTCCACGC TGCTCTCCAC GGGCACCAAC
    ACTACAACAA CGGCCATGGC AGCGGCGGCG GTGGCTGCAT CCTACAGCCA GTTGCAACAG
    GCACCGGTCG GAGGCGCGGC TGGAACGGTT TCTGGGATCG GCATTGGCAT GCAGCAGCCC
    ACCAAGCAGA AGACGAAGAA GAAGTCGCAG CAGGAGCGCA ACACCACCAC CGTCGATGTG
    GAGTCGGTTG CCGGCTATAG GGGCAATGAT CCTGTTGAGC AGCTCGTCAA GTACATTGAA
    AACGATGTCA ACGGTGGCGG AAACAGTGCC GCTGGACAGC GCAAGAAGGA GCGCAAGAAG
    CAAAACAAGC TCAAGAAGAG CAACTCGTTG GAGGAGCTGC GCACCTGCTC AAAAATGGAG
    GTGGACGATT TGAAGCGCCA GTCGGCGACC ACGGAGATGA TGCGACATAA GAAGGGCACG
    AACAATGCAT CCTCGGGCGG ATCGTCGTCG ACGGCCTCTG GTTCCTCAAA CAGTGGCAAG
    CACAACTCGG CATCCGTGGC GGATATTAAC AAAAACTGCA ACAAGGAGCA GCAACAGCAA
    TCGACGCCGA CGGTGCAAGT GCGCAACAGC AACAATCCAG GAACGCAGCA GCGGAAGGGC
    GAGCGTCGGT CCTGGGGCAC CGAGGAGTTG CAGTACCTGG GTGACCACCA GGAAATATCC
    GCTGCCTGGT CGGAGCTGGA GACTGTGGGC CAGAAGGAGC TGCCGCTGGC GCTGCCCGCC
    TTGGCGCGCA TGTCCGAACT GGATGCCCTG AATACCGTCT TGTCGGAGAC CGCTGAGTTC
    CATGTGGTTA CCAAGAAGAA GAAACCAAAG AAGCAGCGTG CTGTTACCAT GGACGATGCG
    GCGGTGGCTG CAGCTGCCGT CACGGGCGGC AACTTACAGC GCATGCAGCA GATCACCAAG
    TCCGCGTCCT CCAACATCAT GTCCCAGCGG ATGCACTACT ATACTCCGTA CAACAGCAAC
    AATGGTGGAG GCAGTGTCAA CTATAAGCAA CAGCAGCAAC AGAGCCAACA GTACCAGGAG
    CACTACCAGG CGCCACAGGG GCAGCACCAC CATCACCACC ATCATCATCA CCACCATCAT
    CACCATCACA GCGGCTCGGC GGTTTCGAAT CAGGTGGATA GCTCACGGCG CAAGTCCACG
    TCGTCTATGC CCCCATCGGA GAAATCTGAC TCCAGTGATC TCGACTCGGT CCACTCCCTG
    CCCATCCAGA CGGGGAAGAA GAAGAGCCTA GGCGGTGGCA ACAACAAACA ACGGGGGGCG
    CCGTCAGCCA GTCAACGCCA GGCCAAGCAA AACAACAATG CTGCAGCCCC CATTTCGTAT
    GCGGATATCG CCCGGAACAA GCAGGAGGCT CTAAACAATG CCACGGCCAG CGACACCGAG
    TTGGAGCTGG GCGACAAGAT GCAGATCAAG AGTGGCGGCA AATCCAAGTC GCGACCCGAT
    TTCCCGGAGC TGCCAGGTGC TGTTACGGCA GTGGCTGCCA GTGGAGGTGC AGCCAATCAG
    GCGACGGTTG TCAGCAGCCA AAATACGCCA CCATCCTCGT CCAGTTCCAT CAGTTATTCG
    CAGAGTCTCA ATGCAACGCC GCCCAGCAGC AGCAGCGATG CGGAGTCCAC CAACTCGCCG
    GAGCTGCTGG CGCAGGTGCA GATACCGGCT TACAGCATGA TCACGCCAAC TCTAACAGCC
    TCCATGATCA GCAGTGTCAG CAGTTCTGTC GCCAGCACCT CTTCCACATC CTGCTCACCG
    CCAGCTCTGC AGAAATCCAA GAGCGTGGAG CACGACACCA GCTACAGCTG CAATAGTAGC
    AACTTGGATC AACAGTATCC GGCACTAGAA AAGACCGTCA AGCGGCATAG CACCAACAAC
    GTGTCTGTGG CCGTCGCAGC CTCCACCTCG GCATCGGCGT CCTCCTCGTT GTACAACTTT
    GCAGCGGCAG CCAAGCAGCA GCTGACAGAG AACACCCATT CGGCCGTATC GCCGCCGGCA
    GCTGTAACAA CTACCTCAAC CGCAGTCGCA GCCTCAGCTC CAGCTCCAAC TACAGTTACA
    GTTCCAGCTG CAGTTACAGT TCCTTGTTAT TCCTCATCCT CCACACACGG CTCGACTCAA
    ACTTCAACTT CTACTGCATC GTCTGCTATA GCAGCTTTAC CCTCGGGCAA GGCCAAGGCC
    AAGCCAAAGG AGCTATCTCC CAGCAGCAGT TGCAGCAAGA AGCCAACGAA GCCTAGTCAA
    GATGTGCCGT CAATCAGTCT GTCCATTTCC ACCACGCCAA GGGCGACGAC CAGCACCAGC
    ACTACTACTA CACAAAAGAC CACGGCTGCC ACGCAGACCG AGGGAGCCAA AAAGCTAATC
    GGCGGGATCC ACAAGCTGCC CAGTAGCAGT TGTATTGCGA AGGGAGCCGG AGCCGTTTTG
    GAGGCCACCG CCGGCAGGCG TGCTGTTATC ATACTCAACG ATGATCGCGA AGCAGGCAGG
    AGCAACAATG AGTTCATATT CGGTGATTTC AACGAGGATG AGCTAAAACT CTTCGACGAC
    AACTTGGATG ATGAGGAGGA GGGCCAGCAC AAGCAGTCTT CAAACAAGAA ACAGCAAACT
    GAAGGGGAAT CTGATGTGAC CCAAGATGAC ACCGAAGACG TGGACCAAGA TCTGGACAAG
    GAACTGGAAT GCCTGGGACG ACGACCAGAG AAGAAGCAAG AGCGACAGCA GGATATCAGC
    GCCAGCAGCG ATCAGCATCT GAATGATTCT GGTGCGGCTT CGGATGCAGT CAACAGCTCA
    GCCAGCCTTG ACATGCTCTC AATTTCTGCA GAGGCGGCAC AATCGTCACC TAATGCGGGG
    GCAACCGCTA CCTCATCCAG CGTCGCCAGT CCCATTAACT CGTCCACGCC CTCGGGTGCT
    TCGTCCGCAT CGGCGAGCAC GTCCACCTCA TCGTCCTCGG TCTCGATTTC ATCATCGTCG
    GGCGGCAACG GTGCCGCTTG TCTTGCATCC GTTTCCGGTA TGCTTGGTGG AGTGGCCAAT
    CAGTCCGGGG ACAGCGGCAT CTATGCTGCC GCCAACACGT CTATTAAGGA GCACGTTAAC
    ACTTTCCTAA GCAAGGCCAG CAGCAGCGAG GAGACGCTGC CGAGTCCCAA CTCCATATCG
    ATGCAACAGC TGGAAACATG CAACGACATC GAGGCGGCCA TCATAGCCGC AGCCCGTGCT
    GCCGCTGCTG CCCGGAGCAC CAGCTGCAGT CGCAGCAACT CTCAGGAGCA GGATGCCTTG
    CAGCCGCAGG TCAAGACCAA GGCGACTCCC AATCCGGAGG CGAAGATGGT TCTGCCTGTG
    TTTGTGACCT ACAACAACAA CAACAACGAC GACGATGACG ACGAGGAGGA CGACGAGAGC
    CTGCAAGAAC TGAGCTTCAT GGCCGACCTT AAGGCGGATG CAGCCACGGA GGATATTGCA
    ATGCAGCCGC CACCGCCTTC ATGTCCGGCG ATGATGACCA ACACGGAACT AATCGTCGAC
    TACATCGCCA AAACCTGGAA TGCCATTGCC AACAGTAAGT ACGTAACGTA CTACAACGAG
    CAAGAGCAGG AGCAGGAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03750
    Clone ID Related Accession (Same CDS sequence) NM_001258589.2 , NM_001169189.3 , NM_001258591.2 , NM_001258588.2 , NM_001169188.3 , NM_001258592.2
    Accession Version NM_001258591.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twenty-four, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_001258591.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTCAAAA AGGCTGAGTA CGACGAAGAG ATCTCTTATC GTGCCATCAA ATCCTGCCTC 
    ACAATCAACC CATCCGCACT AACGTCCAGT GCAGCAACAG GCTCGAGCGG CGGGAGCAGC
    AGCAGTGCAG GGCACAACAG CAGCTATAAT TCCACCAATC ACCGGTCAAC GGATCCCGGC
    CAGCGGCACA AGCAGGGCGG CTCCCTGCAG GATCTCGTTG AGGCGGCGCA CCGCAGCGAA
    CTGGCCACAA CCTCGGCGGC GGCGGCAGCA GCAGCGACAG CGGCAGCAAC CCAACGTTTC
    AACTGTGATT ACCAGCTGAG TGGTCGCTCC AATCGCCGGC AGCAGCAGCA GCAACAGCAG
    CACAACCAAA ACGCCACCTC CTCCGCATCC GCATCCAAAA AGATCAAGAA CTTAAACGTC
    TCGGGCCGGC AGCGCGAAGG AGGCAGCCTG CCCAGCAGTG TTAACTTTGA GCCGGCATCG
    TCTGCCATGG AAGCCAAGCA GAATAAGCAA CAGGCTCAGC CTTCGACAGA AGCTGCAGGT
    GGCTCGTTTT CGGGGTCGGT CGGTAACAAG CACCACCACC GCAGCAGCAC CGGCAGTTGC
    AGCAGCCTGA TAACTCAATC GCGCGATACG GAGGCAAACA ATCAGTTCGA CTTGGACATG
    GACGATGCGA CGGGTGTTGG TGCCAGCGCC GTTGTCGGTG CCAGCGGCGA CCAAGACGAC
    AACATGTTGG AGCAATTGGA TCCTCAGAAC CCCTCGATGA AGTTTCTGAT TGACGCCCTA
    AACGACACGC CATTGGAGGC CAGGTACACC GGTGCCGGCA AAAAGTTCCT AATCGACGAC
    CGGTTGCATT TTTCCGTCCT GGAGCTGTCT GCCAGGAGGA AGCTGCAGCA GCAGGCACAA
    TACCTGGCCG CCCTCACCGG ACAATTTGGT CTCGGCCTGG AGGAGGAGAA GCGCAAGGTA
    GAGGCCGGCT ACGTGTCGCT CAACGACAAC TACACGGCGT GCTCCTCAGT TTACGGCGGC
    GGTGCAGGTT CGCCAGCTGC CGTGGAGGGC AGCAAGCCCT CTACCTCCTC CTCGGCCGTG
    TCTGGCGGTA GCAACTGCGG TGCCTCCTCT GACGCACTGT CTAGCGATCT GCGACCTGCG
    AAGATTGGAC GCATGGTCCC CGCCGCAGCG GCAGTGGCAG CTGCGCCAAT GGGTGGCAAG
    ACGACTACTC GACAGCTAGA CGAGAATGGA AATGCATTGG GCGATGGTTT CGGGGGCAGT
    GCAGCCAGTG GCTCCAGTGG CGCCAGTGGC GCCAGTGCTG CCGCCAGCAG TAGCAACGGC
    AACGGTAACG CAAACCAGTT CACAGCTCTA ATAACCCCCA CGGTGGGATC CAGTGTACCC
    ACCTCTACTG TCGCTCACCG TCAGAATAGT GTCTCCACGC TGCTCTCCAC GGGCACCAAC
    ACTACAACAA CGGCCATGGC AGCGGCGGCG GTGGCTGCAT CCTACAGCCA GTTGCAACAG
    GCACCGGTCG GAGGCGCGGC TGGAACGGTT TCTGGGATCG GCATTGGCAT GCAGCAGCCC
    ACCAAGCAGA AGACGAAGAA GAAGTCGCAG CAGGAGCGCA ACACCACCAC CGTCGATGTG
    GAGTCGGTTG CCGGCTATAG GGGCAATGAT CCTGTTGAGC AGCTCGTCAA GTACATTGAA
    AACGATGTCA ACGGTGGCGG AAACAGTGCC GCTGGACAGC GCAAGAAGGA GCGCAAGAAG
    CAAAACAAGC TCAAGAAGAG CAACTCGTTG GAGGAGCTGC GCACCTGCTC AAAAATGGAG
    GTGGACGATT TGAAGCGCCA GTCGGCGACC ACGGAGATGA TGCGACATAA GAAGGGCACG
    AACAATGCAT CCTCGGGCGG ATCGTCGTCG ACGGCCTCTG GTTCCTCAAA CAGTGGCAAG
    CACAACTCGG CATCCGTGGC GGATATTAAC AAAAACTGCA ACAAGGAGCA GCAACAGCAA
    TCGACGCCGA CGGTGCAAGT GCGCAACAGC AACAATCCAG GAACGCAGCA GCGGAAGGGC
    GAGCGTCGGT CCTGGGGCAC CGAGGAGTTG CAGTACCTGG GTGACCACCA GGAAATATCC
    GCTGCCTGGT CGGAGCTGGA GACTGTGGGC CAGAAGGAGC TGCCGCTGGC GCTGCCCGCC
    TTGGCGCGCA TGTCCGAACT GGATGCCCTG AATACCGTCT TGTCGGAGAC CGCTGAGTTC
    CATGTGGTTA CCAAGAAGAA GAAACCAAAG AAGCAGCGTG CTGTTACCAT GGACGATGCG
    GCGGTGGCTG CAGCTGCCGT CACGGGCGGC AACTTACAGC GCATGCAGCA GATCACCAAG
    TCCGCGTCCT CCAACATCAT GTCCCAGCGG ATGCACTACT ATACTCCGTA CAACAGCAAC
    AATGGTGGAG GCAGTGTCAA CTATAAGCAA CAGCAGCAAC AGAGCCAACA GTACCAGGAG
    CACTACCAGG CGCCACAGGG GCAGCACCAC CATCACCACC ATCATCATCA CCACCATCAT
    CACCATCACA GCGGCTCGGC GGTTTCGAAT CAGGTGGATA GCTCACGGCG CAAGTCCACG
    TCGTCTATGC CCCCATCGGA GAAATCTGAC TCCAGTGATC TCGACTCGGT CCACTCCCTG
    CCCATCCAGA CGGGGAAGAA GAAGAGCCTA GGCGGTGGCA ACAACAAACA ACGGGGGGCG
    CCGTCAGCCA GTCAACGCCA GGCCAAGCAA AACAACAATG CTGCAGCCCC CATTTCGTAT
    GCGGATATCG CCCGGAACAA GCAGGAGGCT CTAAACAATG CCACGGCCAG CGACACCGAG
    TTGGAGCTGG GCGACAAGAT GCAGATCAAG AGTGGCGGCA AATCCAAGTC GCGACCCGAT
    TTCCCGGAGC TGCCAGGTGC TGTTACGGCA GTGGCTGCCA GTGGAGGTGC AGCCAATCAG
    GCGACGGTTG TCAGCAGCCA AAATACGCCA CCATCCTCGT CCAGTTCCAT CAGTTATTCG
    CAGAGTCTCA ATGCAACGCC GCCCAGCAGC AGCAGCGATG CGGAGTCCAC CAACTCGCCG
    GAGCTGCTGG CGCAGGTGCA GATACCGGCT TACAGCATGA TCACGCCAAC TCTAACAGCC
    TCCATGATCA GCAGTGTCAG CAGTTCTGTC GCCAGCACCT CTTCCACATC CTGCTCACCG
    CCAGCTCTGC AGAAATCCAA GAGCGTGGAG CACGACACCA GCTACAGCTG CAATAGTAGC
    AACTTGGATC AACAGTATCC GGCACTAGAA AAGACCGTCA AGCGGCATAG CACCAACAAC
    GTGTCTGTGG CCGTCGCAGC CTCCACCTCG GCATCGGCGT CCTCCTCGTT GTACAACTTT
    GCAGCGGCAG CCAAGCAGCA GCTGACAGAG AACACCCATT CGGCCGTATC GCCGCCGGCA
    GCTGTAACAA CTACCTCAAC CGCAGTCGCA GCCTCAGCTC CAGCTCCAAC TACAGTTACA
    GTTCCAGCTG CAGTTACAGT TCCTTGTTAT TCCTCATCCT CCACACACGG CTCGACTCAA
    ACTTCAACTT CTACTGCATC GTCTGCTATA GCAGCTTTAC CCTCGGGCAA GGCCAAGGCC
    AAGCCAAAGG AGCTATCTCC CAGCAGCAGT TGCAGCAAGA AGCCAACGAA GCCTAGTCAA
    GATGTGCCGT CAATCAGTCT GTCCATTTCC ACCACGCCAA GGGCGACGAC CAGCACCAGC
    ACTACTACTA CACAAAAGAC CACGGCTGCC ACGCAGACCG AGGGAGCCAA AAAGCTAATC
    GGCGGGATCC ACAAGCTGCC CAGTAGCAGT TGTATTGCGA AGGGAGCCGG AGCCGTTTTG
    GAGGCCACCG CCGGCAGGCG TGCTGTTATC ATACTCAACG ATGATCGCGA AGCAGGCAGG
    AGCAACAATG AGTTCATATT CGGTGATTTC AACGAGGATG AGCTAAAACT CTTCGACGAC
    AACTTGGATG ATGAGGAGGA GGGCCAGCAC AAGCAGTCTT CAAACAAGAA ACAGCAAACT
    GAAGGGGAAT CTGATGTGAC CCAAGATGAC ACCGAAGACG TGGACCAAGA TCTGGACAAG
    GAACTGGAAT GCCTGGGACG ACGACCAGAG AAGAAGCAAG AGCGACAGCA GGATATCAGC
    GCCAGCAGCG ATCAGCATCT GAATGATTCT GGTGCGGCTT CGGATGCAGT CAACAGCTCA
    GCCAGCCTTG ACATGCTCTC AATTTCTGCA GAGGCGGCAC AATCGTCACC TAATGCGGGG
    GCAACCGCTA CCTCATCCAG CGTCGCCAGT CCCATTAACT CGTCCACGCC CTCGGGTGCT
    TCGTCCGCAT CGGCGAGCAC GTCCACCTCA TCGTCCTCGG TCTCGATTTC ATCATCGTCG
    GGCGGCAACG GTGCCGCTTG TCTTGCATCC GTTTCCGGTA TGCTTGGTGG AGTGGCCAAT
    CAGTCCGGGG ACAGCGGCAT CTATGCTGCC GCCAACACGT CTATTAAGGA GCACGTTAAC
    ACTTTCCTAA GCAAGGCCAG CAGCAGCGAG GAGACGCTGC CGAGTCCCAA CTCCATATCG
    ATGCAACAGC TGGAAACATG CAACGACATC GAGGCGGCCA TCATAGCCGC AGCCCGTGCT
    GCCGCTGCTG CCCGGAGCAC CAGCTGCAGT CGCAGCAACT CTCAGGAGCA GGATGCCTTG
    CAGCCGCAGG TCAAGACCAA GGCGACTCCC AATCCGGAGG CGAAGATGGT TCTGCCTGTG
    TTTGTGACCT ACAACAACAA CAACAACGAC GACGATGACG ACGAGGAGGA CGACGAGAGC
    CTGCAAGAAC TGAGCTTCAT GGCCGACCTT AAGGCGGATG CAGCCACGGA GGATATTGCA
    ATGCAGCCGC CACCGCCTTC ATGTCCGGCG ATGATGACCA ACACGGAACT AATCGTCGAC
    TACATCGCCA AAACCTGGAA TGCCATTGCC AACAGTAAGT ACGTAACGTA CTACAACGAG
    CAAGAGCAGG AGCAGGAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245520.1
    CDS520..5523
    Translation

    Target ORF information:

    RefSeq Version NM_001258591.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twenty-four (tyf), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258591.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTCAAAA AGGCTGAGTA CGACGAAGAG ATCTCTTATC GTGCCATCAA ATCCTGCCTC 
    ACAATCAACC CATCCGCACT AACGTCCAGT GCAGCAACAG GCTCGAGCGG CGGGAGCAGC
    AGCAGTGCAG GGCACAACAG CAGCTATAAT TCCACCAATC ACCGGTCAAC GGATCCCGGC
    CAGCGGCACA AGCAGGGCGG CTCCCTGCAG GATCTCGTTG AGGCGGCGCA CCGCAGCGAA
    CTGGCCACAA CCTCGGCGGC GGCGGCAGCA GCAGCGACAG CGGCAGCAAC CCAACGTTTC
    AACTGTGATT ACCAGCTGAG TGGTCGCTCC AATCGCCGGC AGCAGCAGCA GCAACAGCAG
    CACAACCAAA ACGCCACCTC CTCCGCATCC GCATCCAAAA AGATCAAGAA CTTAAACGTC
    TCGGGCCGGC AGCGCGAAGG AGGCAGCCTG CCCAGCAGTG TTAACTTTGA GCCGGCATCG
    TCTGCCATGG AAGCCAAGCA GAATAAGCAA CAGGCTCAGC CTTCGACAGA AGCTGCAGGT
    GGCTCGTTTT CGGGGTCGGT CGGTAACAAG CACCACCACC GCAGCAGCAC CGGCAGTTGC
    AGCAGCCTGA TAACTCAATC GCGCGATACG GAGGCAAACA ATCAGTTCGA CTTGGACATG
    GACGATGCGA CGGGTGTTGG TGCCAGCGCC GTTGTCGGTG CCAGCGGCGA CCAAGACGAC
    AACATGTTGG AGCAATTGGA TCCTCAGAAC CCCTCGATGA AGTTTCTGAT TGACGCCCTA
    AACGACACGC CATTGGAGGC CAGGTACACC GGTGCCGGCA AAAAGTTCCT AATCGACGAC
    CGGTTGCATT TTTCCGTCCT GGAGCTGTCT GCCAGGAGGA AGCTGCAGCA GCAGGCACAA
    TACCTGGCCG CCCTCACCGG ACAATTTGGT CTCGGCCTGG AGGAGGAGAA GCGCAAGGTA
    GAGGCCGGCT ACGTGTCGCT CAACGACAAC TACACGGCGT GCTCCTCAGT TTACGGCGGC
    GGTGCAGGTT CGCCAGCTGC CGTGGAGGGC AGCAAGCCCT CTACCTCCTC CTCGGCCGTG
    TCTGGCGGTA GCAACTGCGG TGCCTCCTCT GACGCACTGT CTAGCGATCT GCGACCTGCG
    AAGATTGGAC GCATGGTCCC CGCCGCAGCG GCAGTGGCAG CTGCGCCAAT GGGTGGCAAG
    ACGACTACTC GACAGCTAGA CGAGAATGGA AATGCATTGG GCGATGGTTT CGGGGGCAGT
    GCAGCCAGTG GCTCCAGTGG CGCCAGTGGC GCCAGTGCTG CCGCCAGCAG TAGCAACGGC
    AACGGTAACG CAAACCAGTT CACAGCTCTA ATAACCCCCA CGGTGGGATC CAGTGTACCC
    ACCTCTACTG TCGCTCACCG TCAGAATAGT GTCTCCACGC TGCTCTCCAC GGGCACCAAC
    ACTACAACAA CGGCCATGGC AGCGGCGGCG GTGGCTGCAT CCTACAGCCA GTTGCAACAG
    GCACCGGTCG GAGGCGCGGC TGGAACGGTT TCTGGGATCG GCATTGGCAT GCAGCAGCCC
    ACCAAGCAGA AGACGAAGAA GAAGTCGCAG CAGGAGCGCA ACACCACCAC CGTCGATGTG
    GAGTCGGTTG CCGGCTATAG GGGCAATGAT CCTGTTGAGC AGCTCGTCAA GTACATTGAA
    AACGATGTCA ACGGTGGCGG AAACAGTGCC GCTGGACAGC GCAAGAAGGA GCGCAAGAAG
    CAAAACAAGC TCAAGAAGAG CAACTCGTTG GAGGAGCTGC GCACCTGCTC AAAAATGGAG
    GTGGACGATT TGAAGCGCCA GTCGGCGACC ACGGAGATGA TGCGACATAA GAAGGGCACG
    AACAATGCAT CCTCGGGCGG ATCGTCGTCG ACGGCCTCTG GTTCCTCAAA CAGTGGCAAG
    CACAACTCGG CATCCGTGGC GGATATTAAC AAAAACTGCA ACAAGGAGCA GCAACAGCAA
    TCGACGCCGA CGGTGCAAGT GCGCAACAGC AACAATCCAG GAACGCAGCA GCGGAAGGGC
    GAGCGTCGGT CCTGGGGCAC CGAGGAGTTG CAGTACCTGG GTGACCACCA GGAAATATCC
    GCTGCCTGGT CGGAGCTGGA GACTGTGGGC CAGAAGGAGC TGCCGCTGGC GCTGCCCGCC
    TTGGCGCGCA TGTCCGAACT GGATGCCCTG AATACCGTCT TGTCGGAGAC CGCTGAGTTC
    CATGTGGTTA CCAAGAAGAA GAAACCAAAG AAGCAGCGTG CTGTTACCAT GGACGATGCG
    GCGGTGGCTG CAGCTGCCGT CACGGGCGGC AACTTACAGC GCATGCAGCA GATCACCAAG
    TCCGCGTCCT CCAACATCAT GTCCCAGCGG ATGCACTACT ATACTCCGTA CAACAGCAAC
    AATGGTGGAG GCAGTGTCAA CTATAAGCAA CAGCAGCAAC AGAGCCAACA GTACCAGGAG
    CACTACCAGG CGCCACAGGG GCAGCACCAC CATCACCACC ATCATCATCA CCACCATCAT
    CACCATCACA GCGGCTCGGC GGTTTCGAAT CAGGTGGATA GCTCACGGCG CAAGTCCACG
    TCGTCTATGC CCCCATCGGA GAAATCTGAC TCCAGTGATC TCGACTCGGT CCACTCCCTG
    CCCATCCAGA CGGGGAAGAA GAAGAGCCTA GGCGGTGGCA ACAACAAACA ACGGGGGGCG
    CCGTCAGCCA GTCAACGCCA GGCCAAGCAA AACAACAATG CTGCAGCCCC CATTTCGTAT
    GCGGATATCG CCCGGAACAA GCAGGAGGCT CTAAACAATG CCACGGCCAG CGACACCGAG
    TTGGAGCTGG GCGACAAGAT GCAGATCAAG AGTGGCGGCA AATCCAAGTC GCGACCCGAT
    TTCCCGGAGC TGCCAGGTGC TGTTACGGCA GTGGCTGCCA GTGGAGGTGC AGCCAATCAG
    GCGACGGTTG TCAGCAGCCA AAATACGCCA CCATCCTCGT CCAGTTCCAT CAGTTATTCG
    CAGAGTCTCA ATGCAACGCC GCCCAGCAGC AGCAGCGATG CGGAGTCCAC CAACTCGCCG
    GAGCTGCTGG CGCAGGTGCA GATACCGGCT TACAGCATGA TCACGCCAAC TCTAACAGCC
    TCCATGATCA GCAGTGTCAG CAGTTCTGTC GCCAGCACCT CTTCCACATC CTGCTCACCG
    CCAGCTCTGC AGAAATCCAA GAGCGTGGAG CACGACACCA GCTACAGCTG CAATAGTAGC
    AACTTGGATC AACAGTATCC GGCACTAGAA AAGACCGTCA AGCGGCATAG CACCAACAAC
    GTGTCTGTGG CCGTCGCAGC CTCCACCTCG GCATCGGCGT CCTCCTCGTT GTACAACTTT
    GCAGCGGCAG CCAAGCAGCA GCTGACAGAG AACACCCATT CGGCCGTATC GCCGCCGGCA
    GCTGTAACAA CTACCTCAAC CGCAGTCGCA GCCTCAGCTC CAGCTCCAAC TACAGTTACA
    GTTCCAGCTG CAGTTACAGT TCCTTGTTAT TCCTCATCCT CCACACACGG CTCGACTCAA
    ACTTCAACTT CTACTGCATC GTCTGCTATA GCAGCTTTAC CCTCGGGCAA GGCCAAGGCC
    AAGCCAAAGG AGCTATCTCC CAGCAGCAGT TGCAGCAAGA AGCCAACGAA GCCTAGTCAA
    GATGTGCCGT CAATCAGTCT GTCCATTTCC ACCACGCCAA GGGCGACGAC CAGCACCAGC
    ACTACTACTA CACAAAAGAC CACGGCTGCC ACGCAGACCG AGGGAGCCAA AAAGCTAATC
    GGCGGGATCC ACAAGCTGCC CAGTAGCAGT TGTATTGCGA AGGGAGCCGG AGCCGTTTTG
    GAGGCCACCG CCGGCAGGCG TGCTGTTATC ATACTCAACG ATGATCGCGA AGCAGGCAGG
    AGCAACAATG AGTTCATATT CGGTGATTTC AACGAGGATG AGCTAAAACT CTTCGACGAC
    AACTTGGATG ATGAGGAGGA GGGCCAGCAC AAGCAGTCTT CAAACAAGAA ACAGCAAACT
    GAAGGGGAAT CTGATGTGAC CCAAGATGAC ACCGAAGACG TGGACCAAGA TCTGGACAAG
    GAACTGGAAT GCCTGGGACG ACGACCAGAG AAGAAGCAAG AGCGACAGCA GGATATCAGC
    GCCAGCAGCG ATCAGCATCT GAATGATTCT GGTGCGGCTT CGGATGCAGT CAACAGCTCA
    GCCAGCCTTG ACATGCTCTC AATTTCTGCA GAGGCGGCAC AATCGTCACC TAATGCGGGG
    GCAACCGCTA CCTCATCCAG CGTCGCCAGT CCCATTAACT CGTCCACGCC CTCGGGTGCT
    TCGTCCGCAT CGGCGAGCAC GTCCACCTCA TCGTCCTCGG TCTCGATTTC ATCATCGTCG
    GGCGGCAACG GTGCCGCTTG TCTTGCATCC GTTTCCGGTA TGCTTGGTGG AGTGGCCAAT
    CAGTCCGGGG ACAGCGGCAT CTATGCTGCC GCCAACACGT CTATTAAGGA GCACGTTAAC
    ACTTTCCTAA GCAAGGCCAG CAGCAGCGAG GAGACGCTGC CGAGTCCCAA CTCCATATCG
    ATGCAACAGC TGGAAACATG CAACGACATC GAGGCGGCCA TCATAGCCGC AGCCCGTGCT
    GCCGCTGCTG CCCGGAGCAC CAGCTGCAGT CGCAGCAACT CTCAGGAGCA GGATGCCTTG
    CAGCCGCAGG TCAAGACCAA GGCGACTCCC AATCCGGAGG CGAAGATGGT TCTGCCTGTG
    TTTGTGACCT ACAACAACAA CAACAACGAC GACGATGACG ACGAGGAGGA CGACGAGAGC
    CTGCAAGAAC TGAGCTTCAT GGCCGACCTT AAGGCGGATG CAGCCACGGA GGATATTGCA
    ATGCAGCCGC CACCGCCTTC ATGTCCGGCG ATGATGACCA ACACGGAACT AATCGTCGAC
    TACATCGCCA AAACCTGGAA TGCCATTGCC AACAGTAAGT ACGTAACGTA CTACAACGAG
    CAAGAGCAGG AGCAGGAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03750
    Clone ID Related Accession (Same CDS sequence) NM_001258589.2 , NM_001169189.3 , NM_001258591.2 , NM_001258588.2 , NM_001169188.3 , NM_001258592.2
    Accession Version NM_001258588.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twenty-four, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_001258588.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTCAAAA AGGCTGAGTA CGACGAAGAG ATCTCTTATC GTGCCATCAA ATCCTGCCTC 
    ACAATCAACC CATCCGCACT AACGTCCAGT GCAGCAACAG GCTCGAGCGG CGGGAGCAGC
    AGCAGTGCAG GGCACAACAG CAGCTATAAT TCCACCAATC ACCGGTCAAC GGATCCCGGC
    CAGCGGCACA AGCAGGGCGG CTCCCTGCAG GATCTCGTTG AGGCGGCGCA CCGCAGCGAA
    CTGGCCACAA CCTCGGCGGC GGCGGCAGCA GCAGCGACAG CGGCAGCAAC CCAACGTTTC
    AACTGTGATT ACCAGCTGAG TGGTCGCTCC AATCGCCGGC AGCAGCAGCA GCAACAGCAG
    CACAACCAAA ACGCCACCTC CTCCGCATCC GCATCCAAAA AGATCAAGAA CTTAAACGTC
    TCGGGCCGGC AGCGCGAAGG AGGCAGCCTG CCCAGCAGTG TTAACTTTGA GCCGGCATCG
    TCTGCCATGG AAGCCAAGCA GAATAAGCAA CAGGCTCAGC CTTCGACAGA AGCTGCAGGT
    GGCTCGTTTT CGGGGTCGGT CGGTAACAAG CACCACCACC GCAGCAGCAC CGGCAGTTGC
    AGCAGCCTGA TAACTCAATC GCGCGATACG GAGGCAAACA ATCAGTTCGA CTTGGACATG
    GACGATGCGA CGGGTGTTGG TGCCAGCGCC GTTGTCGGTG CCAGCGGCGA CCAAGACGAC
    AACATGTTGG AGCAATTGGA TCCTCAGAAC CCCTCGATGA AGTTTCTGAT TGACGCCCTA
    AACGACACGC CATTGGAGGC CAGGTACACC GGTGCCGGCA AAAAGTTCCT AATCGACGAC
    CGGTTGCATT TTTCCGTCCT GGAGCTGTCT GCCAGGAGGA AGCTGCAGCA GCAGGCACAA
    TACCTGGCCG CCCTCACCGG ACAATTTGGT CTCGGCCTGG AGGAGGAGAA GCGCAAGGTA
    GAGGCCGGCT ACGTGTCGCT CAACGACAAC TACACGGCGT GCTCCTCAGT TTACGGCGGC
    GGTGCAGGTT CGCCAGCTGC CGTGGAGGGC AGCAAGCCCT CTACCTCCTC CTCGGCCGTG
    TCTGGCGGTA GCAACTGCGG TGCCTCCTCT GACGCACTGT CTAGCGATCT GCGACCTGCG
    AAGATTGGAC GCATGGTCCC CGCCGCAGCG GCAGTGGCAG CTGCGCCAAT GGGTGGCAAG
    ACGACTACTC GACAGCTAGA CGAGAATGGA AATGCATTGG GCGATGGTTT CGGGGGCAGT
    GCAGCCAGTG GCTCCAGTGG CGCCAGTGGC GCCAGTGCTG CCGCCAGCAG TAGCAACGGC
    AACGGTAACG CAAACCAGTT CACAGCTCTA ATAACCCCCA CGGTGGGATC CAGTGTACCC
    ACCTCTACTG TCGCTCACCG TCAGAATAGT GTCTCCACGC TGCTCTCCAC GGGCACCAAC
    ACTACAACAA CGGCCATGGC AGCGGCGGCG GTGGCTGCAT CCTACAGCCA GTTGCAACAG
    GCACCGGTCG GAGGCGCGGC TGGAACGGTT TCTGGGATCG GCATTGGCAT GCAGCAGCCC
    ACCAAGCAGA AGACGAAGAA GAAGTCGCAG CAGGAGCGCA ACACCACCAC CGTCGATGTG
    GAGTCGGTTG CCGGCTATAG GGGCAATGAT CCTGTTGAGC AGCTCGTCAA GTACATTGAA
    AACGATGTCA ACGGTGGCGG AAACAGTGCC GCTGGACAGC GCAAGAAGGA GCGCAAGAAG
    CAAAACAAGC TCAAGAAGAG CAACTCGTTG GAGGAGCTGC GCACCTGCTC AAAAATGGAG
    GTGGACGATT TGAAGCGCCA GTCGGCGACC ACGGAGATGA TGCGACATAA GAAGGGCACG
    AACAATGCAT CCTCGGGCGG ATCGTCGTCG ACGGCCTCTG GTTCCTCAAA CAGTGGCAAG
    CACAACTCGG CATCCGTGGC GGATATTAAC AAAAACTGCA ACAAGGAGCA GCAACAGCAA
    TCGACGCCGA CGGTGCAAGT GCGCAACAGC AACAATCCAG GAACGCAGCA GCGGAAGGGC
    GAGCGTCGGT CCTGGGGCAC CGAGGAGTTG CAGTACCTGG GTGACCACCA GGAAATATCC
    GCTGCCTGGT CGGAGCTGGA GACTGTGGGC CAGAAGGAGC TGCCGCTGGC GCTGCCCGCC
    TTGGCGCGCA TGTCCGAACT GGATGCCCTG AATACCGTCT TGTCGGAGAC CGCTGAGTTC
    CATGTGGTTA CCAAGAAGAA GAAACCAAAG AAGCAGCGTG CTGTTACCAT GGACGATGCG
    GCGGTGGCTG CAGCTGCCGT CACGGGCGGC AACTTACAGC GCATGCAGCA GATCACCAAG
    TCCGCGTCCT CCAACATCAT GTCCCAGCGG ATGCACTACT ATACTCCGTA CAACAGCAAC
    AATGGTGGAG GCAGTGTCAA CTATAAGCAA CAGCAGCAAC AGAGCCAACA GTACCAGGAG
    CACTACCAGG CGCCACAGGG GCAGCACCAC CATCACCACC ATCATCATCA CCACCATCAT
    CACCATCACA GCGGCTCGGC GGTTTCGAAT CAGGTGGATA GCTCACGGCG CAAGTCCACG
    TCGTCTATGC CCCCATCGGA GAAATCTGAC TCCAGTGATC TCGACTCGGT CCACTCCCTG
    CCCATCCAGA CGGGGAAGAA GAAGAGCCTA GGCGGTGGCA ACAACAAACA ACGGGGGGCG
    CCGTCAGCCA GTCAACGCCA GGCCAAGCAA AACAACAATG CTGCAGCCCC CATTTCGTAT
    GCGGATATCG CCCGGAACAA GCAGGAGGCT CTAAACAATG CCACGGCCAG CGACACCGAG
    TTGGAGCTGG GCGACAAGAT GCAGATCAAG AGTGGCGGCA AATCCAAGTC GCGACCCGAT
    TTCCCGGAGC TGCCAGGTGC TGTTACGGCA GTGGCTGCCA GTGGAGGTGC AGCCAATCAG
    GCGACGGTTG TCAGCAGCCA AAATACGCCA CCATCCTCGT CCAGTTCCAT CAGTTATTCG
    CAGAGTCTCA ATGCAACGCC GCCCAGCAGC AGCAGCGATG CGGAGTCCAC CAACTCGCCG
    GAGCTGCTGG CGCAGGTGCA GATACCGGCT TACAGCATGA TCACGCCAAC TCTAACAGCC
    TCCATGATCA GCAGTGTCAG CAGTTCTGTC GCCAGCACCT CTTCCACATC CTGCTCACCG
    CCAGCTCTGC AGAAATCCAA GAGCGTGGAG CACGACACCA GCTACAGCTG CAATAGTAGC
    AACTTGGATC AACAGTATCC GGCACTAGAA AAGACCGTCA AGCGGCATAG CACCAACAAC
    GTGTCTGTGG CCGTCGCAGC CTCCACCTCG GCATCGGCGT CCTCCTCGTT GTACAACTTT
    GCAGCGGCAG CCAAGCAGCA GCTGACAGAG AACACCCATT CGGCCGTATC GCCGCCGGCA
    GCTGTAACAA CTACCTCAAC CGCAGTCGCA GCCTCAGCTC CAGCTCCAAC TACAGTTACA
    GTTCCAGCTG CAGTTACAGT TCCTTGTTAT TCCTCATCCT CCACACACGG CTCGACTCAA
    ACTTCAACTT CTACTGCATC GTCTGCTATA GCAGCTTTAC CCTCGGGCAA GGCCAAGGCC
    AAGCCAAAGG AGCTATCTCC CAGCAGCAGT TGCAGCAAGA AGCCAACGAA GCCTAGTCAA
    GATGTGCCGT CAATCAGTCT GTCCATTTCC ACCACGCCAA GGGCGACGAC CAGCACCAGC
    ACTACTACTA CACAAAAGAC CACGGCTGCC ACGCAGACCG AGGGAGCCAA AAAGCTAATC
    GGCGGGATCC ACAAGCTGCC CAGTAGCAGT TGTATTGCGA AGGGAGCCGG AGCCGTTTTG
    GAGGCCACCG CCGGCAGGCG TGCTGTTATC ATACTCAACG ATGATCGCGA AGCAGGCAGG
    AGCAACAATG AGTTCATATT CGGTGATTTC AACGAGGATG AGCTAAAACT CTTCGACGAC
    AACTTGGATG ATGAGGAGGA GGGCCAGCAC AAGCAGTCTT CAAACAAGAA ACAGCAAACT
    GAAGGGGAAT CTGATGTGAC CCAAGATGAC ACCGAAGACG TGGACCAAGA TCTGGACAAG
    GAACTGGAAT GCCTGGGACG ACGACCAGAG AAGAAGCAAG AGCGACAGCA GGATATCAGC
    GCCAGCAGCG ATCAGCATCT GAATGATTCT GGTGCGGCTT CGGATGCAGT CAACAGCTCA
    GCCAGCCTTG ACATGCTCTC AATTTCTGCA GAGGCGGCAC AATCGTCACC TAATGCGGGG
    GCAACCGCTA CCTCATCCAG CGTCGCCAGT CCCATTAACT CGTCCACGCC CTCGGGTGCT
    TCGTCCGCAT CGGCGAGCAC GTCCACCTCA TCGTCCTCGG TCTCGATTTC ATCATCGTCG
    GGCGGCAACG GTGCCGCTTG TCTTGCATCC GTTTCCGGTA TGCTTGGTGG AGTGGCCAAT
    CAGTCCGGGG ACAGCGGCAT CTATGCTGCC GCCAACACGT CTATTAAGGA GCACGTTAAC
    ACTTTCCTAA GCAAGGCCAG CAGCAGCGAG GAGACGCTGC CGAGTCCCAA CTCCATATCG
    ATGCAACAGC TGGAAACATG CAACGACATC GAGGCGGCCA TCATAGCCGC AGCCCGTGCT
    GCCGCTGCTG CCCGGAGCAC CAGCTGCAGT CGCAGCAACT CTCAGGAGCA GGATGCCTTG
    CAGCCGCAGG TCAAGACCAA GGCGACTCCC AATCCGGAGG CGAAGATGGT TCTGCCTGTG
    TTTGTGACCT ACAACAACAA CAACAACGAC GACGATGACG ACGAGGAGGA CGACGAGAGC
    CTGCAAGAAC TGAGCTTCAT GGCCGACCTT AAGGCGGATG CAGCCACGGA GGATATTGCA
    ATGCAGCCGC CACCGCCTTC ATGTCCGGCG ATGATGACCA ACACGGAACT AATCGTCGAC
    TACATCGCCA AAACCTGGAA TGCCATTGCC AACAGTAAGT ACGTAACGTA CTACAACGAG
    CAAGAGCAGG AGCAGGAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245517.1
    CDS174..5177
    Translation

    Target ORF information:

    RefSeq Version NM_001258588.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twenty-four (tyf), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258588.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTCAAAA AGGCTGAGTA CGACGAAGAG ATCTCTTATC GTGCCATCAA ATCCTGCCTC 
    ACAATCAACC CATCCGCACT AACGTCCAGT GCAGCAACAG GCTCGAGCGG CGGGAGCAGC
    AGCAGTGCAG GGCACAACAG CAGCTATAAT TCCACCAATC ACCGGTCAAC GGATCCCGGC
    CAGCGGCACA AGCAGGGCGG CTCCCTGCAG GATCTCGTTG AGGCGGCGCA CCGCAGCGAA
    CTGGCCACAA CCTCGGCGGC GGCGGCAGCA GCAGCGACAG CGGCAGCAAC CCAACGTTTC
    AACTGTGATT ACCAGCTGAG TGGTCGCTCC AATCGCCGGC AGCAGCAGCA GCAACAGCAG
    CACAACCAAA ACGCCACCTC CTCCGCATCC GCATCCAAAA AGATCAAGAA CTTAAACGTC
    TCGGGCCGGC AGCGCGAAGG AGGCAGCCTG CCCAGCAGTG TTAACTTTGA GCCGGCATCG
    TCTGCCATGG AAGCCAAGCA GAATAAGCAA CAGGCTCAGC CTTCGACAGA AGCTGCAGGT
    GGCTCGTTTT CGGGGTCGGT CGGTAACAAG CACCACCACC GCAGCAGCAC CGGCAGTTGC
    AGCAGCCTGA TAACTCAATC GCGCGATACG GAGGCAAACA ATCAGTTCGA CTTGGACATG
    GACGATGCGA CGGGTGTTGG TGCCAGCGCC GTTGTCGGTG CCAGCGGCGA CCAAGACGAC
    AACATGTTGG AGCAATTGGA TCCTCAGAAC CCCTCGATGA AGTTTCTGAT TGACGCCCTA
    AACGACACGC CATTGGAGGC CAGGTACACC GGTGCCGGCA AAAAGTTCCT AATCGACGAC
    CGGTTGCATT TTTCCGTCCT GGAGCTGTCT GCCAGGAGGA AGCTGCAGCA GCAGGCACAA
    TACCTGGCCG CCCTCACCGG ACAATTTGGT CTCGGCCTGG AGGAGGAGAA GCGCAAGGTA
    GAGGCCGGCT ACGTGTCGCT CAACGACAAC TACACGGCGT GCTCCTCAGT TTACGGCGGC
    GGTGCAGGTT CGCCAGCTGC CGTGGAGGGC AGCAAGCCCT CTACCTCCTC CTCGGCCGTG
    TCTGGCGGTA GCAACTGCGG TGCCTCCTCT GACGCACTGT CTAGCGATCT GCGACCTGCG
    AAGATTGGAC GCATGGTCCC CGCCGCAGCG GCAGTGGCAG CTGCGCCAAT GGGTGGCAAG
    ACGACTACTC GACAGCTAGA CGAGAATGGA AATGCATTGG GCGATGGTTT CGGGGGCAGT
    GCAGCCAGTG GCTCCAGTGG CGCCAGTGGC GCCAGTGCTG CCGCCAGCAG TAGCAACGGC
    AACGGTAACG CAAACCAGTT CACAGCTCTA ATAACCCCCA CGGTGGGATC CAGTGTACCC
    ACCTCTACTG TCGCTCACCG TCAGAATAGT GTCTCCACGC TGCTCTCCAC GGGCACCAAC
    ACTACAACAA CGGCCATGGC AGCGGCGGCG GTGGCTGCAT CCTACAGCCA GTTGCAACAG
    GCACCGGTCG GAGGCGCGGC TGGAACGGTT TCTGGGATCG GCATTGGCAT GCAGCAGCCC
    ACCAAGCAGA AGACGAAGAA GAAGTCGCAG CAGGAGCGCA ACACCACCAC CGTCGATGTG
    GAGTCGGTTG CCGGCTATAG GGGCAATGAT CCTGTTGAGC AGCTCGTCAA GTACATTGAA
    AACGATGTCA ACGGTGGCGG AAACAGTGCC GCTGGACAGC GCAAGAAGGA GCGCAAGAAG
    CAAAACAAGC TCAAGAAGAG CAACTCGTTG GAGGAGCTGC GCACCTGCTC AAAAATGGAG
    GTGGACGATT TGAAGCGCCA GTCGGCGACC ACGGAGATGA TGCGACATAA GAAGGGCACG
    AACAATGCAT CCTCGGGCGG ATCGTCGTCG ACGGCCTCTG GTTCCTCAAA CAGTGGCAAG
    CACAACTCGG CATCCGTGGC GGATATTAAC AAAAACTGCA ACAAGGAGCA GCAACAGCAA
    TCGACGCCGA CGGTGCAAGT GCGCAACAGC AACAATCCAG GAACGCAGCA GCGGAAGGGC
    GAGCGTCGGT CCTGGGGCAC CGAGGAGTTG CAGTACCTGG GTGACCACCA GGAAATATCC
    GCTGCCTGGT CGGAGCTGGA GACTGTGGGC CAGAAGGAGC TGCCGCTGGC GCTGCCCGCC
    TTGGCGCGCA TGTCCGAACT GGATGCCCTG AATACCGTCT TGTCGGAGAC CGCTGAGTTC
    CATGTGGTTA CCAAGAAGAA GAAACCAAAG AAGCAGCGTG CTGTTACCAT GGACGATGCG
    GCGGTGGCTG CAGCTGCCGT CACGGGCGGC AACTTACAGC GCATGCAGCA GATCACCAAG
    TCCGCGTCCT CCAACATCAT GTCCCAGCGG ATGCACTACT ATACTCCGTA CAACAGCAAC
    AATGGTGGAG GCAGTGTCAA CTATAAGCAA CAGCAGCAAC AGAGCCAACA GTACCAGGAG
    CACTACCAGG CGCCACAGGG GCAGCACCAC CATCACCACC ATCATCATCA CCACCATCAT
    CACCATCACA GCGGCTCGGC GGTTTCGAAT CAGGTGGATA GCTCACGGCG CAAGTCCACG
    TCGTCTATGC CCCCATCGGA GAAATCTGAC TCCAGTGATC TCGACTCGGT CCACTCCCTG
    CCCATCCAGA CGGGGAAGAA GAAGAGCCTA GGCGGTGGCA ACAACAAACA ACGGGGGGCG
    CCGTCAGCCA GTCAACGCCA GGCCAAGCAA AACAACAATG CTGCAGCCCC CATTTCGTAT
    GCGGATATCG CCCGGAACAA GCAGGAGGCT CTAAACAATG CCACGGCCAG CGACACCGAG
    TTGGAGCTGG GCGACAAGAT GCAGATCAAG AGTGGCGGCA AATCCAAGTC GCGACCCGAT
    TTCCCGGAGC TGCCAGGTGC TGTTACGGCA GTGGCTGCCA GTGGAGGTGC AGCCAATCAG
    GCGACGGTTG TCAGCAGCCA AAATACGCCA CCATCCTCGT CCAGTTCCAT CAGTTATTCG
    CAGAGTCTCA ATGCAACGCC GCCCAGCAGC AGCAGCGATG CGGAGTCCAC CAACTCGCCG
    GAGCTGCTGG CGCAGGTGCA GATACCGGCT TACAGCATGA TCACGCCAAC TCTAACAGCC
    TCCATGATCA GCAGTGTCAG CAGTTCTGTC GCCAGCACCT CTTCCACATC CTGCTCACCG
    CCAGCTCTGC AGAAATCCAA GAGCGTGGAG CACGACACCA GCTACAGCTG CAATAGTAGC
    AACTTGGATC AACAGTATCC GGCACTAGAA AAGACCGTCA AGCGGCATAG CACCAACAAC
    GTGTCTGTGG CCGTCGCAGC CTCCACCTCG GCATCGGCGT CCTCCTCGTT GTACAACTTT
    GCAGCGGCAG CCAAGCAGCA GCTGACAGAG AACACCCATT CGGCCGTATC GCCGCCGGCA
    GCTGTAACAA CTACCTCAAC CGCAGTCGCA GCCTCAGCTC CAGCTCCAAC TACAGTTACA
    GTTCCAGCTG CAGTTACAGT TCCTTGTTAT TCCTCATCCT CCACACACGG CTCGACTCAA
    ACTTCAACTT CTACTGCATC GTCTGCTATA GCAGCTTTAC CCTCGGGCAA GGCCAAGGCC
    AAGCCAAAGG AGCTATCTCC CAGCAGCAGT TGCAGCAAGA AGCCAACGAA GCCTAGTCAA
    GATGTGCCGT CAATCAGTCT GTCCATTTCC ACCACGCCAA GGGCGACGAC CAGCACCAGC
    ACTACTACTA CACAAAAGAC CACGGCTGCC ACGCAGACCG AGGGAGCCAA AAAGCTAATC
    GGCGGGATCC ACAAGCTGCC CAGTAGCAGT TGTATTGCGA AGGGAGCCGG AGCCGTTTTG
    GAGGCCACCG CCGGCAGGCG TGCTGTTATC ATACTCAACG ATGATCGCGA AGCAGGCAGG
    AGCAACAATG AGTTCATATT CGGTGATTTC AACGAGGATG AGCTAAAACT CTTCGACGAC
    AACTTGGATG ATGAGGAGGA GGGCCAGCAC AAGCAGTCTT CAAACAAGAA ACAGCAAACT
    GAAGGGGAAT CTGATGTGAC CCAAGATGAC ACCGAAGACG TGGACCAAGA TCTGGACAAG
    GAACTGGAAT GCCTGGGACG ACGACCAGAG AAGAAGCAAG AGCGACAGCA GGATATCAGC
    GCCAGCAGCG ATCAGCATCT GAATGATTCT GGTGCGGCTT CGGATGCAGT CAACAGCTCA
    GCCAGCCTTG ACATGCTCTC AATTTCTGCA GAGGCGGCAC AATCGTCACC TAATGCGGGG
    GCAACCGCTA CCTCATCCAG CGTCGCCAGT CCCATTAACT CGTCCACGCC CTCGGGTGCT
    TCGTCCGCAT CGGCGAGCAC GTCCACCTCA TCGTCCTCGG TCTCGATTTC ATCATCGTCG
    GGCGGCAACG GTGCCGCTTG TCTTGCATCC GTTTCCGGTA TGCTTGGTGG AGTGGCCAAT
    CAGTCCGGGG ACAGCGGCAT CTATGCTGCC GCCAACACGT CTATTAAGGA GCACGTTAAC
    ACTTTCCTAA GCAAGGCCAG CAGCAGCGAG GAGACGCTGC CGAGTCCCAA CTCCATATCG
    ATGCAACAGC TGGAAACATG CAACGACATC GAGGCGGCCA TCATAGCCGC AGCCCGTGCT
    GCCGCTGCTG CCCGGAGCAC CAGCTGCAGT CGCAGCAACT CTCAGGAGCA GGATGCCTTG
    CAGCCGCAGG TCAAGACCAA GGCGACTCCC AATCCGGAGG CGAAGATGGT TCTGCCTGTG
    TTTGTGACCT ACAACAACAA CAACAACGAC GACGATGACG ACGAGGAGGA CGACGAGAGC
    CTGCAAGAAC TGAGCTTCAT GGCCGACCTT AAGGCGGATG CAGCCACGGA GGATATTGCA
    ATGCAGCCGC CACCGCCTTC ATGTCCGGCG ATGATGACCA ACACGGAACT AATCGTCGAC
    TACATCGCCA AAACCTGGAA TGCCATTGCC AACAGTAAGT ACGTAACGTA CTACAACGAG
    CAAGAGCAGG AGCAGGAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03750
    Clone ID Related Accession (Same CDS sequence) NM_001258589.2 , NM_001169189.3 , NM_001258591.2 , NM_001258588.2 , NM_001169188.3 , NM_001258592.2
    Accession Version NM_001169188.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twenty-four, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_001169188.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTCAAAA AGGCTGAGTA CGACGAAGAG ATCTCTTATC GTGCCATCAA ATCCTGCCTC 
    ACAATCAACC CATCCGCACT AACGTCCAGT GCAGCAACAG GCTCGAGCGG CGGGAGCAGC
    AGCAGTGCAG GGCACAACAG CAGCTATAAT TCCACCAATC ACCGGTCAAC GGATCCCGGC
    CAGCGGCACA AGCAGGGCGG CTCCCTGCAG GATCTCGTTG AGGCGGCGCA CCGCAGCGAA
    CTGGCCACAA CCTCGGCGGC GGCGGCAGCA GCAGCGACAG CGGCAGCAAC CCAACGTTTC
    AACTGTGATT ACCAGCTGAG TGGTCGCTCC AATCGCCGGC AGCAGCAGCA GCAACAGCAG
    CACAACCAAA ACGCCACCTC CTCCGCATCC GCATCCAAAA AGATCAAGAA CTTAAACGTC
    TCGGGCCGGC AGCGCGAAGG AGGCAGCCTG CCCAGCAGTG TTAACTTTGA GCCGGCATCG
    TCTGCCATGG AAGCCAAGCA GAATAAGCAA CAGGCTCAGC CTTCGACAGA AGCTGCAGGT
    GGCTCGTTTT CGGGGTCGGT CGGTAACAAG CACCACCACC GCAGCAGCAC CGGCAGTTGC
    AGCAGCCTGA TAACTCAATC GCGCGATACG GAGGCAAACA ATCAGTTCGA CTTGGACATG
    GACGATGCGA CGGGTGTTGG TGCCAGCGCC GTTGTCGGTG CCAGCGGCGA CCAAGACGAC
    AACATGTTGG AGCAATTGGA TCCTCAGAAC CCCTCGATGA AGTTTCTGAT TGACGCCCTA
    AACGACACGC CATTGGAGGC CAGGTACACC GGTGCCGGCA AAAAGTTCCT AATCGACGAC
    CGGTTGCATT TTTCCGTCCT GGAGCTGTCT GCCAGGAGGA AGCTGCAGCA GCAGGCACAA
    TACCTGGCCG CCCTCACCGG ACAATTTGGT CTCGGCCTGG AGGAGGAGAA GCGCAAGGTA
    GAGGCCGGCT ACGTGTCGCT CAACGACAAC TACACGGCGT GCTCCTCAGT TTACGGCGGC
    GGTGCAGGTT CGCCAGCTGC CGTGGAGGGC AGCAAGCCCT CTACCTCCTC CTCGGCCGTG
    TCTGGCGGTA GCAACTGCGG TGCCTCCTCT GACGCACTGT CTAGCGATCT GCGACCTGCG
    AAGATTGGAC GCATGGTCCC CGCCGCAGCG GCAGTGGCAG CTGCGCCAAT GGGTGGCAAG
    ACGACTACTC GACAGCTAGA CGAGAATGGA AATGCATTGG GCGATGGTTT CGGGGGCAGT
    GCAGCCAGTG GCTCCAGTGG CGCCAGTGGC GCCAGTGCTG CCGCCAGCAG TAGCAACGGC
    AACGGTAACG CAAACCAGTT CACAGCTCTA ATAACCCCCA CGGTGGGATC CAGTGTACCC
    ACCTCTACTG TCGCTCACCG TCAGAATAGT GTCTCCACGC TGCTCTCCAC GGGCACCAAC
    ACTACAACAA CGGCCATGGC AGCGGCGGCG GTGGCTGCAT CCTACAGCCA GTTGCAACAG
    GCACCGGTCG GAGGCGCGGC TGGAACGGTT TCTGGGATCG GCATTGGCAT GCAGCAGCCC
    ACCAAGCAGA AGACGAAGAA GAAGTCGCAG CAGGAGCGCA ACACCACCAC CGTCGATGTG
    GAGTCGGTTG CCGGCTATAG GGGCAATGAT CCTGTTGAGC AGCTCGTCAA GTACATTGAA
    AACGATGTCA ACGGTGGCGG AAACAGTGCC GCTGGACAGC GCAAGAAGGA GCGCAAGAAG
    CAAAACAAGC TCAAGAAGAG CAACTCGTTG GAGGAGCTGC GCACCTGCTC AAAAATGGAG
    GTGGACGATT TGAAGCGCCA GTCGGCGACC ACGGAGATGA TGCGACATAA GAAGGGCACG
    AACAATGCAT CCTCGGGCGG ATCGTCGTCG ACGGCCTCTG GTTCCTCAAA CAGTGGCAAG
    CACAACTCGG CATCCGTGGC GGATATTAAC AAAAACTGCA ACAAGGAGCA GCAACAGCAA
    TCGACGCCGA CGGTGCAAGT GCGCAACAGC AACAATCCAG GAACGCAGCA GCGGAAGGGC
    GAGCGTCGGT CCTGGGGCAC CGAGGAGTTG CAGTACCTGG GTGACCACCA GGAAATATCC
    GCTGCCTGGT CGGAGCTGGA GACTGTGGGC CAGAAGGAGC TGCCGCTGGC GCTGCCCGCC
    TTGGCGCGCA TGTCCGAACT GGATGCCCTG AATACCGTCT TGTCGGAGAC CGCTGAGTTC
    CATGTGGTTA CCAAGAAGAA GAAACCAAAG AAGCAGCGTG CTGTTACCAT GGACGATGCG
    GCGGTGGCTG CAGCTGCCGT CACGGGCGGC AACTTACAGC GCATGCAGCA GATCACCAAG
    TCCGCGTCCT CCAACATCAT GTCCCAGCGG ATGCACTACT ATACTCCGTA CAACAGCAAC
    AATGGTGGAG GCAGTGTCAA CTATAAGCAA CAGCAGCAAC AGAGCCAACA GTACCAGGAG
    CACTACCAGG CGCCACAGGG GCAGCACCAC CATCACCACC ATCATCATCA CCACCATCAT
    CACCATCACA GCGGCTCGGC GGTTTCGAAT CAGGTGGATA GCTCACGGCG CAAGTCCACG
    TCGTCTATGC CCCCATCGGA GAAATCTGAC TCCAGTGATC TCGACTCGGT CCACTCCCTG
    CCCATCCAGA CGGGGAAGAA GAAGAGCCTA GGCGGTGGCA ACAACAAACA ACGGGGGGCG
    CCGTCAGCCA GTCAACGCCA GGCCAAGCAA AACAACAATG CTGCAGCCCC CATTTCGTAT
    GCGGATATCG CCCGGAACAA GCAGGAGGCT CTAAACAATG CCACGGCCAG CGACACCGAG
    TTGGAGCTGG GCGACAAGAT GCAGATCAAG AGTGGCGGCA AATCCAAGTC GCGACCCGAT
    TTCCCGGAGC TGCCAGGTGC TGTTACGGCA GTGGCTGCCA GTGGAGGTGC AGCCAATCAG
    GCGACGGTTG TCAGCAGCCA AAATACGCCA CCATCCTCGT CCAGTTCCAT CAGTTATTCG
    CAGAGTCTCA ATGCAACGCC GCCCAGCAGC AGCAGCGATG CGGAGTCCAC CAACTCGCCG
    GAGCTGCTGG CGCAGGTGCA GATACCGGCT TACAGCATGA TCACGCCAAC TCTAACAGCC
    TCCATGATCA GCAGTGTCAG CAGTTCTGTC GCCAGCACCT CTTCCACATC CTGCTCACCG
    CCAGCTCTGC AGAAATCCAA GAGCGTGGAG CACGACACCA GCTACAGCTG CAATAGTAGC
    AACTTGGATC AACAGTATCC GGCACTAGAA AAGACCGTCA AGCGGCATAG CACCAACAAC
    GTGTCTGTGG CCGTCGCAGC CTCCACCTCG GCATCGGCGT CCTCCTCGTT GTACAACTTT
    GCAGCGGCAG CCAAGCAGCA GCTGACAGAG AACACCCATT CGGCCGTATC GCCGCCGGCA
    GCTGTAACAA CTACCTCAAC CGCAGTCGCA GCCTCAGCTC CAGCTCCAAC TACAGTTACA
    GTTCCAGCTG CAGTTACAGT TCCTTGTTAT TCCTCATCCT CCACACACGG CTCGACTCAA
    ACTTCAACTT CTACTGCATC GTCTGCTATA GCAGCTTTAC CCTCGGGCAA GGCCAAGGCC
    AAGCCAAAGG AGCTATCTCC CAGCAGCAGT TGCAGCAAGA AGCCAACGAA GCCTAGTCAA
    GATGTGCCGT CAATCAGTCT GTCCATTTCC ACCACGCCAA GGGCGACGAC CAGCACCAGC
    ACTACTACTA CACAAAAGAC CACGGCTGCC ACGCAGACCG AGGGAGCCAA AAAGCTAATC
    GGCGGGATCC ACAAGCTGCC CAGTAGCAGT TGTATTGCGA AGGGAGCCGG AGCCGTTTTG
    GAGGCCACCG CCGGCAGGCG TGCTGTTATC ATACTCAACG ATGATCGCGA AGCAGGCAGG
    AGCAACAATG AGTTCATATT CGGTGATTTC AACGAGGATG AGCTAAAACT CTTCGACGAC
    AACTTGGATG ATGAGGAGGA GGGCCAGCAC AAGCAGTCTT CAAACAAGAA ACAGCAAACT
    GAAGGGGAAT CTGATGTGAC CCAAGATGAC ACCGAAGACG TGGACCAAGA TCTGGACAAG
    GAACTGGAAT GCCTGGGACG ACGACCAGAG AAGAAGCAAG AGCGACAGCA GGATATCAGC
    GCCAGCAGCG ATCAGCATCT GAATGATTCT GGTGCGGCTT CGGATGCAGT CAACAGCTCA
    GCCAGCCTTG ACATGCTCTC AATTTCTGCA GAGGCGGCAC AATCGTCACC TAATGCGGGG
    GCAACCGCTA CCTCATCCAG CGTCGCCAGT CCCATTAACT CGTCCACGCC CTCGGGTGCT
    TCGTCCGCAT CGGCGAGCAC GTCCACCTCA TCGTCCTCGG TCTCGATTTC ATCATCGTCG
    GGCGGCAACG GTGCCGCTTG TCTTGCATCC GTTTCCGGTA TGCTTGGTGG AGTGGCCAAT
    CAGTCCGGGG ACAGCGGCAT CTATGCTGCC GCCAACACGT CTATTAAGGA GCACGTTAAC
    ACTTTCCTAA GCAAGGCCAG CAGCAGCGAG GAGACGCTGC CGAGTCCCAA CTCCATATCG
    ATGCAACAGC TGGAAACATG CAACGACATC GAGGCGGCCA TCATAGCCGC AGCCCGTGCT
    GCCGCTGCTG CCCGGAGCAC CAGCTGCAGT CGCAGCAACT CTCAGGAGCA GGATGCCTTG
    CAGCCGCAGG TCAAGACCAA GGCGACTCCC AATCCGGAGG CGAAGATGGT TCTGCCTGTG
    TTTGTGACCT ACAACAACAA CAACAACGAC GACGATGACG ACGAGGAGGA CGACGAGAGC
    CTGCAAGAAC TGAGCTTCAT GGCCGACCTT AAGGCGGATG CAGCCACGGA GGATATTGCA
    ATGCAGCCGC CACCGCCTTC ATGTCCGGCG ATGATGACCA ACACGGAACT AATCGTCGAC
    TACATCGCCA AAACCTGGAA TGCCATTGCC AACAGTAAGT ACGTAACGTA CTACAACGAG
    CAAGAGCAGG AGCAGGAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162659.2
    CDS331..5334
    Translation

    Target ORF information:

    RefSeq Version NM_001169188.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twenty-four (tyf), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169188.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTCAAAA AGGCTGAGTA CGACGAAGAG ATCTCTTATC GTGCCATCAA ATCCTGCCTC 
    ACAATCAACC CATCCGCACT AACGTCCAGT GCAGCAACAG GCTCGAGCGG CGGGAGCAGC
    AGCAGTGCAG GGCACAACAG CAGCTATAAT TCCACCAATC ACCGGTCAAC GGATCCCGGC
    CAGCGGCACA AGCAGGGCGG CTCCCTGCAG GATCTCGTTG AGGCGGCGCA CCGCAGCGAA
    CTGGCCACAA CCTCGGCGGC GGCGGCAGCA GCAGCGACAG CGGCAGCAAC CCAACGTTTC
    AACTGTGATT ACCAGCTGAG TGGTCGCTCC AATCGCCGGC AGCAGCAGCA GCAACAGCAG
    CACAACCAAA ACGCCACCTC CTCCGCATCC GCATCCAAAA AGATCAAGAA CTTAAACGTC
    TCGGGCCGGC AGCGCGAAGG AGGCAGCCTG CCCAGCAGTG TTAACTTTGA GCCGGCATCG
    TCTGCCATGG AAGCCAAGCA GAATAAGCAA CAGGCTCAGC CTTCGACAGA AGCTGCAGGT
    GGCTCGTTTT CGGGGTCGGT CGGTAACAAG CACCACCACC GCAGCAGCAC CGGCAGTTGC
    AGCAGCCTGA TAACTCAATC GCGCGATACG GAGGCAAACA ATCAGTTCGA CTTGGACATG
    GACGATGCGA CGGGTGTTGG TGCCAGCGCC GTTGTCGGTG CCAGCGGCGA CCAAGACGAC
    AACATGTTGG AGCAATTGGA TCCTCAGAAC CCCTCGATGA AGTTTCTGAT TGACGCCCTA
    AACGACACGC CATTGGAGGC CAGGTACACC GGTGCCGGCA AAAAGTTCCT AATCGACGAC
    CGGTTGCATT TTTCCGTCCT GGAGCTGTCT GCCAGGAGGA AGCTGCAGCA GCAGGCACAA
    TACCTGGCCG CCCTCACCGG ACAATTTGGT CTCGGCCTGG AGGAGGAGAA GCGCAAGGTA
    GAGGCCGGCT ACGTGTCGCT CAACGACAAC TACACGGCGT GCTCCTCAGT TTACGGCGGC
    GGTGCAGGTT CGCCAGCTGC CGTGGAGGGC AGCAAGCCCT CTACCTCCTC CTCGGCCGTG
    TCTGGCGGTA GCAACTGCGG TGCCTCCTCT GACGCACTGT CTAGCGATCT GCGACCTGCG
    AAGATTGGAC GCATGGTCCC CGCCGCAGCG GCAGTGGCAG CTGCGCCAAT GGGTGGCAAG
    ACGACTACTC GACAGCTAGA CGAGAATGGA AATGCATTGG GCGATGGTTT CGGGGGCAGT
    GCAGCCAGTG GCTCCAGTGG CGCCAGTGGC GCCAGTGCTG CCGCCAGCAG TAGCAACGGC
    AACGGTAACG CAAACCAGTT CACAGCTCTA ATAACCCCCA CGGTGGGATC CAGTGTACCC
    ACCTCTACTG TCGCTCACCG TCAGAATAGT GTCTCCACGC TGCTCTCCAC GGGCACCAAC
    ACTACAACAA CGGCCATGGC AGCGGCGGCG GTGGCTGCAT CCTACAGCCA GTTGCAACAG
    GCACCGGTCG GAGGCGCGGC TGGAACGGTT TCTGGGATCG GCATTGGCAT GCAGCAGCCC
    ACCAAGCAGA AGACGAAGAA GAAGTCGCAG CAGGAGCGCA ACACCACCAC CGTCGATGTG
    GAGTCGGTTG CCGGCTATAG GGGCAATGAT CCTGTTGAGC AGCTCGTCAA GTACATTGAA
    AACGATGTCA ACGGTGGCGG AAACAGTGCC GCTGGACAGC GCAAGAAGGA GCGCAAGAAG
    CAAAACAAGC TCAAGAAGAG CAACTCGTTG GAGGAGCTGC GCACCTGCTC AAAAATGGAG
    GTGGACGATT TGAAGCGCCA GTCGGCGACC ACGGAGATGA TGCGACATAA GAAGGGCACG
    AACAATGCAT CCTCGGGCGG ATCGTCGTCG ACGGCCTCTG GTTCCTCAAA CAGTGGCAAG
    CACAACTCGG CATCCGTGGC GGATATTAAC AAAAACTGCA ACAAGGAGCA GCAACAGCAA
    TCGACGCCGA CGGTGCAAGT GCGCAACAGC AACAATCCAG GAACGCAGCA GCGGAAGGGC
    GAGCGTCGGT CCTGGGGCAC CGAGGAGTTG CAGTACCTGG GTGACCACCA GGAAATATCC
    GCTGCCTGGT CGGAGCTGGA GACTGTGGGC CAGAAGGAGC TGCCGCTGGC GCTGCCCGCC
    TTGGCGCGCA TGTCCGAACT GGATGCCCTG AATACCGTCT TGTCGGAGAC CGCTGAGTTC
    CATGTGGTTA CCAAGAAGAA GAAACCAAAG AAGCAGCGTG CTGTTACCAT GGACGATGCG
    GCGGTGGCTG CAGCTGCCGT CACGGGCGGC AACTTACAGC GCATGCAGCA GATCACCAAG
    TCCGCGTCCT CCAACATCAT GTCCCAGCGG ATGCACTACT ATACTCCGTA CAACAGCAAC
    AATGGTGGAG GCAGTGTCAA CTATAAGCAA CAGCAGCAAC AGAGCCAACA GTACCAGGAG
    CACTACCAGG CGCCACAGGG GCAGCACCAC CATCACCACC ATCATCATCA CCACCATCAT
    CACCATCACA GCGGCTCGGC GGTTTCGAAT CAGGTGGATA GCTCACGGCG CAAGTCCACG
    TCGTCTATGC CCCCATCGGA GAAATCTGAC TCCAGTGATC TCGACTCGGT CCACTCCCTG
    CCCATCCAGA CGGGGAAGAA GAAGAGCCTA GGCGGTGGCA ACAACAAACA ACGGGGGGCG
    CCGTCAGCCA GTCAACGCCA GGCCAAGCAA AACAACAATG CTGCAGCCCC CATTTCGTAT
    GCGGATATCG CCCGGAACAA GCAGGAGGCT CTAAACAATG CCACGGCCAG CGACACCGAG
    TTGGAGCTGG GCGACAAGAT GCAGATCAAG AGTGGCGGCA AATCCAAGTC GCGACCCGAT
    TTCCCGGAGC TGCCAGGTGC TGTTACGGCA GTGGCTGCCA GTGGAGGTGC AGCCAATCAG
    GCGACGGTTG TCAGCAGCCA AAATACGCCA CCATCCTCGT CCAGTTCCAT CAGTTATTCG
    CAGAGTCTCA ATGCAACGCC GCCCAGCAGC AGCAGCGATG CGGAGTCCAC CAACTCGCCG
    GAGCTGCTGG CGCAGGTGCA GATACCGGCT TACAGCATGA TCACGCCAAC TCTAACAGCC
    TCCATGATCA GCAGTGTCAG CAGTTCTGTC GCCAGCACCT CTTCCACATC CTGCTCACCG
    CCAGCTCTGC AGAAATCCAA GAGCGTGGAG CACGACACCA GCTACAGCTG CAATAGTAGC
    AACTTGGATC AACAGTATCC GGCACTAGAA AAGACCGTCA AGCGGCATAG CACCAACAAC
    GTGTCTGTGG CCGTCGCAGC CTCCACCTCG GCATCGGCGT CCTCCTCGTT GTACAACTTT
    GCAGCGGCAG CCAAGCAGCA GCTGACAGAG AACACCCATT CGGCCGTATC GCCGCCGGCA
    GCTGTAACAA CTACCTCAAC CGCAGTCGCA GCCTCAGCTC CAGCTCCAAC TACAGTTACA
    GTTCCAGCTG CAGTTACAGT TCCTTGTTAT TCCTCATCCT CCACACACGG CTCGACTCAA
    ACTTCAACTT CTACTGCATC GTCTGCTATA GCAGCTTTAC CCTCGGGCAA GGCCAAGGCC
    AAGCCAAAGG AGCTATCTCC CAGCAGCAGT TGCAGCAAGA AGCCAACGAA GCCTAGTCAA
    GATGTGCCGT CAATCAGTCT GTCCATTTCC ACCACGCCAA GGGCGACGAC CAGCACCAGC
    ACTACTACTA CACAAAAGAC CACGGCTGCC ACGCAGACCG AGGGAGCCAA AAAGCTAATC
    GGCGGGATCC ACAAGCTGCC CAGTAGCAGT TGTATTGCGA AGGGAGCCGG AGCCGTTTTG
    GAGGCCACCG CCGGCAGGCG TGCTGTTATC ATACTCAACG ATGATCGCGA AGCAGGCAGG
    AGCAACAATG AGTTCATATT CGGTGATTTC AACGAGGATG AGCTAAAACT CTTCGACGAC
    AACTTGGATG ATGAGGAGGA GGGCCAGCAC AAGCAGTCTT CAAACAAGAA ACAGCAAACT
    GAAGGGGAAT CTGATGTGAC CCAAGATGAC ACCGAAGACG TGGACCAAGA TCTGGACAAG
    GAACTGGAAT GCCTGGGACG ACGACCAGAG AAGAAGCAAG AGCGACAGCA GGATATCAGC
    GCCAGCAGCG ATCAGCATCT GAATGATTCT GGTGCGGCTT CGGATGCAGT CAACAGCTCA
    GCCAGCCTTG ACATGCTCTC AATTTCTGCA GAGGCGGCAC AATCGTCACC TAATGCGGGG
    GCAACCGCTA CCTCATCCAG CGTCGCCAGT CCCATTAACT CGTCCACGCC CTCGGGTGCT
    TCGTCCGCAT CGGCGAGCAC GTCCACCTCA TCGTCCTCGG TCTCGATTTC ATCATCGTCG
    GGCGGCAACG GTGCCGCTTG TCTTGCATCC GTTTCCGGTA TGCTTGGTGG AGTGGCCAAT
    CAGTCCGGGG ACAGCGGCAT CTATGCTGCC GCCAACACGT CTATTAAGGA GCACGTTAAC
    ACTTTCCTAA GCAAGGCCAG CAGCAGCGAG GAGACGCTGC CGAGTCCCAA CTCCATATCG
    ATGCAACAGC TGGAAACATG CAACGACATC GAGGCGGCCA TCATAGCCGC AGCCCGTGCT
    GCCGCTGCTG CCCGGAGCAC CAGCTGCAGT CGCAGCAACT CTCAGGAGCA GGATGCCTTG
    CAGCCGCAGG TCAAGACCAA GGCGACTCCC AATCCGGAGG CGAAGATGGT TCTGCCTGTG
    TTTGTGACCT ACAACAACAA CAACAACGAC GACGATGACG ACGAGGAGGA CGACGAGAGC
    CTGCAAGAAC TGAGCTTCAT GGCCGACCTT AAGGCGGATG CAGCCACGGA GGATATTGCA
    ATGCAGCCGC CACCGCCTTC ATGTCCGGCG ATGATGACCA ACACGGAACT AATCGTCGAC
    TACATCGCCA AAACCTGGAA TGCCATTGCC AACAGTAAGT ACGTAACGTA CTACAACGAG
    CAAGAGCAGG AGCAGGAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03750
    Clone ID Related Accession (Same CDS sequence) NM_001258589.2 , NM_001169189.3 , NM_001258591.2 , NM_001258588.2 , NM_001169188.3 , NM_001258592.2
    Accession Version NM_001258592.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5004bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product twenty-four, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_001258592.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTCAAAA AGGCTGAGTA CGACGAAGAG ATCTCTTATC GTGCCATCAA ATCCTGCCTC 
    ACAATCAACC CATCCGCACT AACGTCCAGT GCAGCAACAG GCTCGAGCGG CGGGAGCAGC
    AGCAGTGCAG GGCACAACAG CAGCTATAAT TCCACCAATC ACCGGTCAAC GGATCCCGGC
    CAGCGGCACA AGCAGGGCGG CTCCCTGCAG GATCTCGTTG AGGCGGCGCA CCGCAGCGAA
    CTGGCCACAA CCTCGGCGGC GGCGGCAGCA GCAGCGACAG CGGCAGCAAC CCAACGTTTC
    AACTGTGATT ACCAGCTGAG TGGTCGCTCC AATCGCCGGC AGCAGCAGCA GCAACAGCAG
    CACAACCAAA ACGCCACCTC CTCCGCATCC GCATCCAAAA AGATCAAGAA CTTAAACGTC
    TCGGGCCGGC AGCGCGAAGG AGGCAGCCTG CCCAGCAGTG TTAACTTTGA GCCGGCATCG
    TCTGCCATGG AAGCCAAGCA GAATAAGCAA CAGGCTCAGC CTTCGACAGA AGCTGCAGGT
    GGCTCGTTTT CGGGGTCGGT CGGTAACAAG CACCACCACC GCAGCAGCAC CGGCAGTTGC
    AGCAGCCTGA TAACTCAATC GCGCGATACG GAGGCAAACA ATCAGTTCGA CTTGGACATG
    GACGATGCGA CGGGTGTTGG TGCCAGCGCC GTTGTCGGTG CCAGCGGCGA CCAAGACGAC
    AACATGTTGG AGCAATTGGA TCCTCAGAAC CCCTCGATGA AGTTTCTGAT TGACGCCCTA
    AACGACACGC CATTGGAGGC CAGGTACACC GGTGCCGGCA AAAAGTTCCT AATCGACGAC
    CGGTTGCATT TTTCCGTCCT GGAGCTGTCT GCCAGGAGGA AGCTGCAGCA GCAGGCACAA
    TACCTGGCCG CCCTCACCGG ACAATTTGGT CTCGGCCTGG AGGAGGAGAA GCGCAAGGTA
    GAGGCCGGCT ACGTGTCGCT CAACGACAAC TACACGGCGT GCTCCTCAGT TTACGGCGGC
    GGTGCAGGTT CGCCAGCTGC CGTGGAGGGC AGCAAGCCCT CTACCTCCTC CTCGGCCGTG
    TCTGGCGGTA GCAACTGCGG TGCCTCCTCT GACGCACTGT CTAGCGATCT GCGACCTGCG
    AAGATTGGAC GCATGGTCCC CGCCGCAGCG GCAGTGGCAG CTGCGCCAAT GGGTGGCAAG
    ACGACTACTC GACAGCTAGA CGAGAATGGA AATGCATTGG GCGATGGTTT CGGGGGCAGT
    GCAGCCAGTG GCTCCAGTGG CGCCAGTGGC GCCAGTGCTG CCGCCAGCAG TAGCAACGGC
    AACGGTAACG CAAACCAGTT CACAGCTCTA ATAACCCCCA CGGTGGGATC CAGTGTACCC
    ACCTCTACTG TCGCTCACCG TCAGAATAGT GTCTCCACGC TGCTCTCCAC GGGCACCAAC
    ACTACAACAA CGGCCATGGC AGCGGCGGCG GTGGCTGCAT CCTACAGCCA GTTGCAACAG
    GCACCGGTCG GAGGCGCGGC TGGAACGGTT TCTGGGATCG GCATTGGCAT GCAGCAGCCC
    ACCAAGCAGA AGACGAAGAA GAAGTCGCAG CAGGAGCGCA ACACCACCAC CGTCGATGTG
    GAGTCGGTTG CCGGCTATAG GGGCAATGAT CCTGTTGAGC AGCTCGTCAA GTACATTGAA
    AACGATGTCA ACGGTGGCGG AAACAGTGCC GCTGGACAGC GCAAGAAGGA GCGCAAGAAG
    CAAAACAAGC TCAAGAAGAG CAACTCGTTG GAGGAGCTGC GCACCTGCTC AAAAATGGAG
    GTGGACGATT TGAAGCGCCA GTCGGCGACC ACGGAGATGA TGCGACATAA GAAGGGCACG
    AACAATGCAT CCTCGGGCGG ATCGTCGTCG ACGGCCTCTG GTTCCTCAAA CAGTGGCAAG
    CACAACTCGG CATCCGTGGC GGATATTAAC AAAAACTGCA ACAAGGAGCA GCAACAGCAA
    TCGACGCCGA CGGTGCAAGT GCGCAACAGC AACAATCCAG GAACGCAGCA GCGGAAGGGC
    GAGCGTCGGT CCTGGGGCAC CGAGGAGTTG CAGTACCTGG GTGACCACCA GGAAATATCC
    GCTGCCTGGT CGGAGCTGGA GACTGTGGGC CAGAAGGAGC TGCCGCTGGC GCTGCCCGCC
    TTGGCGCGCA TGTCCGAACT GGATGCCCTG AATACCGTCT TGTCGGAGAC CGCTGAGTTC
    CATGTGGTTA CCAAGAAGAA GAAACCAAAG AAGCAGCGTG CTGTTACCAT GGACGATGCG
    GCGGTGGCTG CAGCTGCCGT CACGGGCGGC AACTTACAGC GCATGCAGCA GATCACCAAG
    TCCGCGTCCT CCAACATCAT GTCCCAGCGG ATGCACTACT ATACTCCGTA CAACAGCAAC
    AATGGTGGAG GCAGTGTCAA CTATAAGCAA CAGCAGCAAC AGAGCCAACA GTACCAGGAG
    CACTACCAGG CGCCACAGGG GCAGCACCAC CATCACCACC ATCATCATCA CCACCATCAT
    CACCATCACA GCGGCTCGGC GGTTTCGAAT CAGGTGGATA GCTCACGGCG CAAGTCCACG
    TCGTCTATGC CCCCATCGGA GAAATCTGAC TCCAGTGATC TCGACTCGGT CCACTCCCTG
    CCCATCCAGA CGGGGAAGAA GAAGAGCCTA GGCGGTGGCA ACAACAAACA ACGGGGGGCG
    CCGTCAGCCA GTCAACGCCA GGCCAAGCAA AACAACAATG CTGCAGCCCC CATTTCGTAT
    GCGGATATCG CCCGGAACAA GCAGGAGGCT CTAAACAATG CCACGGCCAG CGACACCGAG
    TTGGAGCTGG GCGACAAGAT GCAGATCAAG AGTGGCGGCA AATCCAAGTC GCGACCCGAT
    TTCCCGGAGC TGCCAGGTGC TGTTACGGCA GTGGCTGCCA GTGGAGGTGC AGCCAATCAG
    GCGACGGTTG TCAGCAGCCA AAATACGCCA CCATCCTCGT CCAGTTCCAT CAGTTATTCG
    CAGAGTCTCA ATGCAACGCC GCCCAGCAGC AGCAGCGATG CGGAGTCCAC CAACTCGCCG
    GAGCTGCTGG CGCAGGTGCA GATACCGGCT TACAGCATGA TCACGCCAAC TCTAACAGCC
    TCCATGATCA GCAGTGTCAG CAGTTCTGTC GCCAGCACCT CTTCCACATC CTGCTCACCG
    CCAGCTCTGC AGAAATCCAA GAGCGTGGAG CACGACACCA GCTACAGCTG CAATAGTAGC
    AACTTGGATC AACAGTATCC GGCACTAGAA AAGACCGTCA AGCGGCATAG CACCAACAAC
    GTGTCTGTGG CCGTCGCAGC CTCCACCTCG GCATCGGCGT CCTCCTCGTT GTACAACTTT
    GCAGCGGCAG CCAAGCAGCA GCTGACAGAG AACACCCATT CGGCCGTATC GCCGCCGGCA
    GCTGTAACAA CTACCTCAAC CGCAGTCGCA GCCTCAGCTC CAGCTCCAAC TACAGTTACA
    GTTCCAGCTG CAGTTACAGT TCCTTGTTAT TCCTCATCCT CCACACACGG CTCGACTCAA
    ACTTCAACTT CTACTGCATC GTCTGCTATA GCAGCTTTAC CCTCGGGCAA GGCCAAGGCC
    AAGCCAAAGG AGCTATCTCC CAGCAGCAGT TGCAGCAAGA AGCCAACGAA GCCTAGTCAA
    GATGTGCCGT CAATCAGTCT GTCCATTTCC ACCACGCCAA GGGCGACGAC CAGCACCAGC
    ACTACTACTA CACAAAAGAC CACGGCTGCC ACGCAGACCG AGGGAGCCAA AAAGCTAATC
    GGCGGGATCC ACAAGCTGCC CAGTAGCAGT TGTATTGCGA AGGGAGCCGG AGCCGTTTTG
    GAGGCCACCG CCGGCAGGCG TGCTGTTATC ATACTCAACG ATGATCGCGA AGCAGGCAGG
    AGCAACAATG AGTTCATATT CGGTGATTTC AACGAGGATG AGCTAAAACT CTTCGACGAC
    AACTTGGATG ATGAGGAGGA GGGCCAGCAC AAGCAGTCTT CAAACAAGAA ACAGCAAACT
    GAAGGGGAAT CTGATGTGAC CCAAGATGAC ACCGAAGACG TGGACCAAGA TCTGGACAAG
    GAACTGGAAT GCCTGGGACG ACGACCAGAG AAGAAGCAAG AGCGACAGCA GGATATCAGC
    GCCAGCAGCG ATCAGCATCT GAATGATTCT GGTGCGGCTT CGGATGCAGT CAACAGCTCA
    GCCAGCCTTG ACATGCTCTC AATTTCTGCA GAGGCGGCAC AATCGTCACC TAATGCGGGG
    GCAACCGCTA CCTCATCCAG CGTCGCCAGT CCCATTAACT CGTCCACGCC CTCGGGTGCT
    TCGTCCGCAT CGGCGAGCAC GTCCACCTCA TCGTCCTCGG TCTCGATTTC ATCATCGTCG
    GGCGGCAACG GTGCCGCTTG TCTTGCATCC GTTTCCGGTA TGCTTGGTGG AGTGGCCAAT
    CAGTCCGGGG ACAGCGGCAT CTATGCTGCC GCCAACACGT CTATTAAGGA GCACGTTAAC
    ACTTTCCTAA GCAAGGCCAG CAGCAGCGAG GAGACGCTGC CGAGTCCCAA CTCCATATCG
    ATGCAACAGC TGGAAACATG CAACGACATC GAGGCGGCCA TCATAGCCGC AGCCCGTGCT
    GCCGCTGCTG CCCGGAGCAC CAGCTGCAGT CGCAGCAACT CTCAGGAGCA GGATGCCTTG
    CAGCCGCAGG TCAAGACCAA GGCGACTCCC AATCCGGAGG CGAAGATGGT TCTGCCTGTG
    TTTGTGACCT ACAACAACAA CAACAACGAC GACGATGACG ACGAGGAGGA CGACGAGAGC
    CTGCAAGAAC TGAGCTTCAT GGCCGACCTT AAGGCGGATG CAGCCACGGA GGATATTGCA
    ATGCAGCCGC CACCGCCTTC ATGTCCGGCG ATGATGACCA ACACGGAACT AATCGTCGAC
    TACATCGCCA AAACCTGGAA TGCCATTGCC AACAGTAAGT ACGTAACGTA CTACAACGAG
    CAAGAGCAGG AGCAGGAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245521.1
    CDS308..5311
    Translation

    Target ORF information:

    RefSeq Version NM_001258592.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster twenty-four (tyf), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258592.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTCAAAA AGGCTGAGTA CGACGAAGAG ATCTCTTATC GTGCCATCAA ATCCTGCCTC 
    ACAATCAACC CATCCGCACT AACGTCCAGT GCAGCAACAG GCTCGAGCGG CGGGAGCAGC
    AGCAGTGCAG GGCACAACAG CAGCTATAAT TCCACCAATC ACCGGTCAAC GGATCCCGGC
    CAGCGGCACA AGCAGGGCGG CTCCCTGCAG GATCTCGTTG AGGCGGCGCA CCGCAGCGAA
    CTGGCCACAA CCTCGGCGGC GGCGGCAGCA GCAGCGACAG CGGCAGCAAC CCAACGTTTC
    AACTGTGATT ACCAGCTGAG TGGTCGCTCC AATCGCCGGC AGCAGCAGCA GCAACAGCAG
    CACAACCAAA ACGCCACCTC CTCCGCATCC GCATCCAAAA AGATCAAGAA CTTAAACGTC
    TCGGGCCGGC AGCGCGAAGG AGGCAGCCTG CCCAGCAGTG TTAACTTTGA GCCGGCATCG
    TCTGCCATGG AAGCCAAGCA GAATAAGCAA CAGGCTCAGC CTTCGACAGA AGCTGCAGGT
    GGCTCGTTTT CGGGGTCGGT CGGTAACAAG CACCACCACC GCAGCAGCAC CGGCAGTTGC
    AGCAGCCTGA TAACTCAATC GCGCGATACG GAGGCAAACA ATCAGTTCGA CTTGGACATG
    GACGATGCGA CGGGTGTTGG TGCCAGCGCC GTTGTCGGTG CCAGCGGCGA CCAAGACGAC
    AACATGTTGG AGCAATTGGA TCCTCAGAAC CCCTCGATGA AGTTTCTGAT TGACGCCCTA
    AACGACACGC CATTGGAGGC CAGGTACACC GGTGCCGGCA AAAAGTTCCT AATCGACGAC
    CGGTTGCATT TTTCCGTCCT GGAGCTGTCT GCCAGGAGGA AGCTGCAGCA GCAGGCACAA
    TACCTGGCCG CCCTCACCGG ACAATTTGGT CTCGGCCTGG AGGAGGAGAA GCGCAAGGTA
    GAGGCCGGCT ACGTGTCGCT CAACGACAAC TACACGGCGT GCTCCTCAGT TTACGGCGGC
    GGTGCAGGTT CGCCAGCTGC CGTGGAGGGC AGCAAGCCCT CTACCTCCTC CTCGGCCGTG
    TCTGGCGGTA GCAACTGCGG TGCCTCCTCT GACGCACTGT CTAGCGATCT GCGACCTGCG
    AAGATTGGAC GCATGGTCCC CGCCGCAGCG GCAGTGGCAG CTGCGCCAAT GGGTGGCAAG
    ACGACTACTC GACAGCTAGA CGAGAATGGA AATGCATTGG GCGATGGTTT CGGGGGCAGT
    GCAGCCAGTG GCTCCAGTGG CGCCAGTGGC GCCAGTGCTG CCGCCAGCAG TAGCAACGGC
    AACGGTAACG CAAACCAGTT CACAGCTCTA ATAACCCCCA CGGTGGGATC CAGTGTACCC
    ACCTCTACTG TCGCTCACCG TCAGAATAGT GTCTCCACGC TGCTCTCCAC GGGCACCAAC
    ACTACAACAA CGGCCATGGC AGCGGCGGCG GTGGCTGCAT CCTACAGCCA GTTGCAACAG
    GCACCGGTCG GAGGCGCGGC TGGAACGGTT TCTGGGATCG GCATTGGCAT GCAGCAGCCC
    ACCAAGCAGA AGACGAAGAA GAAGTCGCAG CAGGAGCGCA ACACCACCAC CGTCGATGTG
    GAGTCGGTTG CCGGCTATAG GGGCAATGAT CCTGTTGAGC AGCTCGTCAA GTACATTGAA
    AACGATGTCA ACGGTGGCGG AAACAGTGCC GCTGGACAGC GCAAGAAGGA GCGCAAGAAG
    CAAAACAAGC TCAAGAAGAG CAACTCGTTG GAGGAGCTGC GCACCTGCTC AAAAATGGAG
    GTGGACGATT TGAAGCGCCA GTCGGCGACC ACGGAGATGA TGCGACATAA GAAGGGCACG
    AACAATGCAT CCTCGGGCGG ATCGTCGTCG ACGGCCTCTG GTTCCTCAAA CAGTGGCAAG
    CACAACTCGG CATCCGTGGC GGATATTAAC AAAAACTGCA ACAAGGAGCA GCAACAGCAA
    TCGACGCCGA CGGTGCAAGT GCGCAACAGC AACAATCCAG GAACGCAGCA GCGGAAGGGC
    GAGCGTCGGT CCTGGGGCAC CGAGGAGTTG CAGTACCTGG GTGACCACCA GGAAATATCC
    GCTGCCTGGT CGGAGCTGGA GACTGTGGGC CAGAAGGAGC TGCCGCTGGC GCTGCCCGCC
    TTGGCGCGCA TGTCCGAACT GGATGCCCTG AATACCGTCT TGTCGGAGAC CGCTGAGTTC
    CATGTGGTTA CCAAGAAGAA GAAACCAAAG AAGCAGCGTG CTGTTACCAT GGACGATGCG
    GCGGTGGCTG CAGCTGCCGT CACGGGCGGC AACTTACAGC GCATGCAGCA GATCACCAAG
    TCCGCGTCCT CCAACATCAT GTCCCAGCGG ATGCACTACT ATACTCCGTA CAACAGCAAC
    AATGGTGGAG GCAGTGTCAA CTATAAGCAA CAGCAGCAAC AGAGCCAACA GTACCAGGAG
    CACTACCAGG CGCCACAGGG GCAGCACCAC CATCACCACC ATCATCATCA CCACCATCAT
    CACCATCACA GCGGCTCGGC GGTTTCGAAT CAGGTGGATA GCTCACGGCG CAAGTCCACG
    TCGTCTATGC CCCCATCGGA GAAATCTGAC TCCAGTGATC TCGACTCGGT CCACTCCCTG
    CCCATCCAGA CGGGGAAGAA GAAGAGCCTA GGCGGTGGCA ACAACAAACA ACGGGGGGCG
    CCGTCAGCCA GTCAACGCCA GGCCAAGCAA AACAACAATG CTGCAGCCCC CATTTCGTAT
    GCGGATATCG CCCGGAACAA GCAGGAGGCT CTAAACAATG CCACGGCCAG CGACACCGAG
    TTGGAGCTGG GCGACAAGAT GCAGATCAAG AGTGGCGGCA AATCCAAGTC GCGACCCGAT
    TTCCCGGAGC TGCCAGGTGC TGTTACGGCA GTGGCTGCCA GTGGAGGTGC AGCCAATCAG
    GCGACGGTTG TCAGCAGCCA AAATACGCCA CCATCCTCGT CCAGTTCCAT CAGTTATTCG
    CAGAGTCTCA ATGCAACGCC GCCCAGCAGC AGCAGCGATG CGGAGTCCAC CAACTCGCCG
    GAGCTGCTGG CGCAGGTGCA GATACCGGCT TACAGCATGA TCACGCCAAC TCTAACAGCC
    TCCATGATCA GCAGTGTCAG CAGTTCTGTC GCCAGCACCT CTTCCACATC CTGCTCACCG
    CCAGCTCTGC AGAAATCCAA GAGCGTGGAG CACGACACCA GCTACAGCTG CAATAGTAGC
    AACTTGGATC AACAGTATCC GGCACTAGAA AAGACCGTCA AGCGGCATAG CACCAACAAC
    GTGTCTGTGG CCGTCGCAGC CTCCACCTCG GCATCGGCGT CCTCCTCGTT GTACAACTTT
    GCAGCGGCAG CCAAGCAGCA GCTGACAGAG AACACCCATT CGGCCGTATC GCCGCCGGCA
    GCTGTAACAA CTACCTCAAC CGCAGTCGCA GCCTCAGCTC CAGCTCCAAC TACAGTTACA
    GTTCCAGCTG CAGTTACAGT TCCTTGTTAT TCCTCATCCT CCACACACGG CTCGACTCAA
    ACTTCAACTT CTACTGCATC GTCTGCTATA GCAGCTTTAC CCTCGGGCAA GGCCAAGGCC
    AAGCCAAAGG AGCTATCTCC CAGCAGCAGT TGCAGCAAGA AGCCAACGAA GCCTAGTCAA
    GATGTGCCGT CAATCAGTCT GTCCATTTCC ACCACGCCAA GGGCGACGAC CAGCACCAGC
    ACTACTACTA CACAAAAGAC CACGGCTGCC ACGCAGACCG AGGGAGCCAA AAAGCTAATC
    GGCGGGATCC ACAAGCTGCC CAGTAGCAGT TGTATTGCGA AGGGAGCCGG AGCCGTTTTG
    GAGGCCACCG CCGGCAGGCG TGCTGTTATC ATACTCAACG ATGATCGCGA AGCAGGCAGG
    AGCAACAATG AGTTCATATT CGGTGATTTC AACGAGGATG AGCTAAAACT CTTCGACGAC
    AACTTGGATG ATGAGGAGGA GGGCCAGCAC AAGCAGTCTT CAAACAAGAA ACAGCAAACT
    GAAGGGGAAT CTGATGTGAC CCAAGATGAC ACCGAAGACG TGGACCAAGA TCTGGACAAG
    GAACTGGAAT GCCTGGGACG ACGACCAGAG AAGAAGCAAG AGCGACAGCA GGATATCAGC
    GCCAGCAGCG ATCAGCATCT GAATGATTCT GGTGCGGCTT CGGATGCAGT CAACAGCTCA
    GCCAGCCTTG ACATGCTCTC AATTTCTGCA GAGGCGGCAC AATCGTCACC TAATGCGGGG
    GCAACCGCTA CCTCATCCAG CGTCGCCAGT CCCATTAACT CGTCCACGCC CTCGGGTGCT
    TCGTCCGCAT CGGCGAGCAC GTCCACCTCA TCGTCCTCGG TCTCGATTTC ATCATCGTCG
    GGCGGCAACG GTGCCGCTTG TCTTGCATCC GTTTCCGGTA TGCTTGGTGG AGTGGCCAAT
    CAGTCCGGGG ACAGCGGCAT CTATGCTGCC GCCAACACGT CTATTAAGGA GCACGTTAAC
    ACTTTCCTAA GCAAGGCCAG CAGCAGCGAG GAGACGCTGC CGAGTCCCAA CTCCATATCG
    ATGCAACAGC TGGAAACATG CAACGACATC GAGGCGGCCA TCATAGCCGC AGCCCGTGCT
    GCCGCTGCTG CCCGGAGCAC CAGCTGCAGT CGCAGCAACT CTCAGGAGCA GGATGCCTTG
    CAGCCGCAGG TCAAGACCAA GGCGACTCCC AATCCGGAGG CGAAGATGGT TCTGCCTGTG
    TTTGTGACCT ACAACAACAA CAACAACGAC GACGATGACG ACGAGGAGGA CGACGAGAGC
    CTGCAAGAAC TGAGCTTCAT GGCCGACCTT AAGGCGGATG CAGCCACGGA GGATATTGCA
    ATGCAGCCGC CACCGCCTTC ATGTCCGGCG ATGATGACCA ACACGGAACT AATCGTCGAC
    TACATCGCCA AAACCTGGAA TGCCATTGCC AACAGTAAGT ACGTAACGTA CTACAACGAG
    CAAGAGCAGG AGCAGGAGAC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.