Vap33 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vap33
Entrez Gene ID	31349
Full Name	VAMP-associated protein 33kDa
Synonyms	BcDNA:GM03307,CG5014,DVAP,DVAP-33A,DVAP33A,Dmel\CG5014,VAP,VAP-33,VAP-33-1,VAP33-A,VAPB,Vap-33-1,Vap-33A,Vap33-1,anon-EST:Liang-2.42,anon-WO03040301.124,clone 2.42,dVAP,dVAP33A,dvap,dvap33a,l(1)G0231,vap-33A,vap33-1
General protein information	Preferred Names VAMP-associated protein 33kDa Names CG5014 gene product from transcript CG5014-RE CG5014-PA CG5014-PB CG5014-PC CG5014-PE VAMP-associated protein of 33kDa A VAMP-associated protein of 33kDa ortholog A Vap-33-1 Vap33-1 Vap33-PA Vap33-PB Vap33-PC Vap33-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3F9-3F9

mRNA and Protein(s)

mRNA	Protein	Name
NM_206625.2	NP_996348.1	VAMP-associated protein 33kDa, isoform A
NM_206623.2	NP_996346.2	VAMP-associated protein 33kDa, isoform E
NM_130731.3	NP_570087.1	VAMP-associated protein 33kDa, isoform B
NM_206624.2	NP_996347.1	VAMP-associated protein 33kDa, isoform C

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	MGC76271	NP_988905.1
Homo sapiens (human)	VAPB	NP_004729.1
Mus musculus (house mouse)	Vapb	NP_062780.2
Rattus norvegicus (Norway rat)	Vapb	NP_068619.1
Gallus gallus (chicken)	VAPB	NP_001006296.1
Drosophila melanogaster (fruit fly)	Vap-33-1	NP_996347.1
Canis lupus familiaris (dog)	VAPB	XP_534466.2
Danio rerio (zebrafish)	vapb	NP_997812.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000626	XP_310452.5
Pan troglodytes (chimpanzee)	VAPB	XP_001135464.1
Bos taurus (cattle)	VAPB	NP_001075892.1
Caenorhabditis elegans (roundworm)	vpr-1	NP_491704.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the relationship between DVAP-P58 phenotypes and the Drosophila modifiers, raises the possibility that pharmacological manipulation of these genes may assist in developing treatments to ameliorate or prevent ALS-related abnormalities
Title: Network analyses reveal novel aspects of ALS pathogenesis.

We propose that Vap function is required in neurons to selectively traffic proteins to axons, and disruption of this function may contribute to the pathology of ALS.
Title: Drosophila Vap-33 is required for axonal localization of Dscam isoforms.

findings suggest VAP(P58S) may function as a dominant negative; study also demonstrated that the ALS8 mutation in dVAP33A interferes with BMP signaling pathways at the neuromuscular junction, identifying a new mechanism underlying pathogenesis of ALS8
Title: A Drosophila model of ALS: human ALS-associated mutation in VAP33A suggests a dominant negative mechanism.

Drosophila VAMP-associated protein of 33 kDa A (DVAP-33A) is the structural homologue of hVAPB and regulates synaptic remodeling by affecting the size and number of boutons at neuromuscular junctions.
Title: hVAPB, the causative gene of a heterogeneous group of motor neuron diseases in humans, is functionally interchangeable with its Drosophila homologue DVAP-33A at the neuromuscular junction.

The following Vap33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vap33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00741	NM_206623.2 Latest version!	Drosophila melanogaster VAMP-associated protein 33kDa (Vap33), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00741	NM_130731.3 Latest version!	Drosophila melanogaster VAMP-associated protein 33kDa (Vap33), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00741	NM_206624.2 Latest version!	Drosophila melanogaster VAMP-associated protein 33kDa (Vap33), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00742	NM_206625.2 Latest version!	Drosophila melanogaster VAMP-associated protein 33kDa (Vap33), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vap33 ( NM_206623.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996346.2 Drosophila melanogaster VAMP-associated protein 33kDa (Vap33), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa00741
Clone ID Related Accession (Same CDS sequence)	NM_206623.2 , NM_130731.3 , NM_206624.2
Accession Version	NM_206623.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	VAMP-associated protein 33kDa, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206623.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAAAT CACTCTTTGA TCTTCCGTTG ACCATTGAAC CAGAACATGA GTTGCGTTTT 
GTGGGTCCCT TCACCCGACC CGTTGTCACA ATCATGACTC TGCGCAACAA CTCGGCTCTG 
CCTCTGGTCT TCAAGATCAA GACAACCGCC CCGAAACGCT ACTGCGTACG TCCAAACATC 
GGCAAGATAA TTCCCTTTCG ATCAACCCAG GTGGAGATCT GCCTTCAGCC ATTCGTCTAC 
GATCAGCAGG AGAAGAACAA GCACAAGTTC ATGGTGCAGA GCGTCCTGGC ACCCATGGAT 
GCTGATCTAA GCGATTTAAA TAAATTGTGG AAGGATCTGG AGCCCGAGCA GCTGATGGAC 
GCCAAACTGA AGTGCGTTTT CGAGATGCCC ACCGCTGAGG CAAATGCTGA GAACACCAGC 
GGTGGTGGTG CCGTTGGCGG CGGAACCGGA GCTGCCGGAG GCGGAAGCGC GGGTGCCAAT 
ACTAGCTCAG CCAGCGCTGA GGCGCTCGAG AGCAAGCCGA AGCTCTCCAG CGAGGATAAG 
TTTAAGCCAT CCAATTTGCT CGAAACGTCT GAGAGTCTGG ACTTGCTGTC CGGAGAGATC 
AAAGCGCTGC GTGAATGCAA CATTGAATTG CGAAGAGAGA ATCTTCACTT GAAGGATCAA 
ATCACACGTT TCCGGAGCTC GCCGGCCGTC AAACAGGTGA ATGAGCCCTA TGCCCCAGTC 
CTGGCTGAGA AGCAGATTCC GGTCTTTTAC ATTGCAGTTG CCATTGCTGC GGCCATCGTT 
AGCCTCCTGC TGGGCAAATT CTTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996346.2
CDS	568..1377
Translation	MSKSLFDLPLTIEPEHELRFVGPFTRPVVTIMTLRNNSALPLVFKIKTTAPKRYCVRPNIGKIIPFRSTQVEICLQPFVYDQQEKNKHKFMVQSVLAPMDADLSDLNKLWKDLEPEQLMDAKLKCVFEMPTAEANAENTSGGGAVGGGTGAAGGGSAGANTSSASAEALESKPKLSSEDKFKPSNLLETSESLDLLSGEIKALRECNIELRRENLHLKDQITRFRSSPAVKQVNEPYAPVLAEKQIPVFYIAVAIAAAIVSLLLGKFFL

Target ORF information:

RefSeq Version	NM_206623.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster VAMP-associated protein 33kDa (Vap33), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206623.2

ORF Insert Sequence:

ATGAGCAAAT CACTCTTTGA TCTTCCGTTG ACCATTGAAC CAGAACATGA GTTGCGTTTT 
GTGGGTCCCT TCACCCGACC CGTTGTCACA ATCATGACTC TGCGCAACAA CTCGGCTCTG 
CCTCTGGTCT TCAAGATCAA GACAACCGCC CCGAAACGCT ACTGCGTACG TCCAAACATC 
GGCAAGATAA TTCCCTTTCG ATCAACCCAG GTGGAGATCT GCCTTCAGCC ATTCGTCTAC 
GATCAGCAGG AGAAGAACAA GCACAAGTTC ATGGTGCAGA GCGTCCTGGC ACCCATGGAT 
GCTGATCTAA GCGATTTAAA TAAATTGTGG AAGGATCTGG AGCCCGAGCA GCTGATGGAC 
GCCAAACTGA AGTGCGTTTT CGAGATGCCC ACCGCTGAGG CAAATGCTGA GAACACCAGC 
GGTGGTGGTG CCGTTGGCGG CGGAACCGGA GCTGCCGGAG GCGGAAGCGC GGGTGCCAAT 
ACTAGCTCAG CCAGCGCTGA GGCGCTCGAG AGCAAGCCGA AGCTCTCCAG CGAGGATAAG 
TTTAAGCCAT CCAATTTGCT CGAAACGTCT GAGAGTCTGG ACTTGCTGTC CGGAGAGATC 
AAAGCGCTGC GTGAATGCAA CATTGAATTG CGAAGAGAGA ATCTTCACTT GAAGGATCAA 
ATCACACGTT TCCGGAGCTC GCCGGCCGTC AAACAGGTGA ATGAGCCCTA TGCCCCAGTC 
CTGGCTGAGA AGCAGATTCC GGTCTTTTAC ATTGCAGTTG CCATTGCTGC GGCCATCGTT 
AGCCTCCTGC TGGGCAAATT CTTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vap33 ( NM_130731.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_570087.1 Drosophila melanogaster VAMP-associated protein 33kDa (Vap33), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00741
Clone ID Related Accession (Same CDS sequence)	NM_206623.2 , NM_130731.3 , NM_206624.2
Accession Version	NM_130731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	VAMP-associated protein 33kDa, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_130731.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAAAT CACTCTTTGA TCTTCCGTTG ACCATTGAAC CAGAACATGA GTTGCGTTTT 
GTGGGTCCCT TCACCCGACC CGTTGTCACA ATCATGACTC TGCGCAACAA CTCGGCTCTG 
CCTCTGGTCT TCAAGATCAA GACAACCGCC CCGAAACGCT ACTGCGTACG TCCAAACATC 
GGCAAGATAA TTCCCTTTCG ATCAACCCAG GTGGAGATCT GCCTTCAGCC ATTCGTCTAC 
GATCAGCAGG AGAAGAACAA GCACAAGTTC ATGGTGCAGA GCGTCCTGGC ACCCATGGAT 
GCTGATCTAA GCGATTTAAA TAAATTGTGG AAGGATCTGG AGCCCGAGCA GCTGATGGAC 
GCCAAACTGA AGTGCGTTTT CGAGATGCCC ACCGCTGAGG CAAATGCTGA GAACACCAGC 
GGTGGTGGTG CCGTTGGCGG CGGAACCGGA GCTGCCGGAG GCGGAAGCGC GGGTGCCAAT 
ACTAGCTCAG CCAGCGCTGA GGCGCTCGAG AGCAAGCCGA AGCTCTCCAG CGAGGATAAG 
TTTAAGCCAT CCAATTTGCT CGAAACGTCT GAGAGTCTGG ACTTGCTGTC CGGAGAGATC 
AAAGCGCTGC GTGAATGCAA CATTGAATTG CGAAGAGAGA ATCTTCACTT GAAGGATCAA 
ATCACACGTT TCCGGAGCTC GCCGGCCGTC AAACAGGTGA ATGAGCCCTA TGCCCCAGTC 
CTGGCTGAGA AGCAGATTCC GGTCTTTTAC ATTGCAGTTG CCATTGCTGC GGCCATCGTT 
AGCCTCCTGC TGGGCAAATT CTTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570087.1
CDS	568..1377
Translation	MSKSLFDLPLTIEPEHELRFVGPFTRPVVTIMTLRNNSALPLVFKIKTTAPKRYCVRPNIGKIIPFRSTQVEICLQPFVYDQQEKNKHKFMVQSVLAPMDADLSDLNKLWKDLEPEQLMDAKLKCVFEMPTAEANAENTSGGGAVGGGTGAAGGGSAGANTSSASAEALESKPKLSSEDKFKPSNLLETSESLDLLSGEIKALRECNIELRRENLHLKDQITRFRSSPAVKQVNEPYAPVLAEKQIPVFYIAVAIAAAIVSLLLGKFFL

Target ORF information:

RefSeq Version	NM_130731.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster VAMP-associated protein 33kDa (Vap33), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130731.3

ORF Insert Sequence:

ATGAGCAAAT CACTCTTTGA TCTTCCGTTG ACCATTGAAC CAGAACATGA GTTGCGTTTT 
GTGGGTCCCT TCACCCGACC CGTTGTCACA ATCATGACTC TGCGCAACAA CTCGGCTCTG 
CCTCTGGTCT TCAAGATCAA GACAACCGCC CCGAAACGCT ACTGCGTACG TCCAAACATC 
GGCAAGATAA TTCCCTTTCG ATCAACCCAG GTGGAGATCT GCCTTCAGCC ATTCGTCTAC 
GATCAGCAGG AGAAGAACAA GCACAAGTTC ATGGTGCAGA GCGTCCTGGC ACCCATGGAT 
GCTGATCTAA GCGATTTAAA TAAATTGTGG AAGGATCTGG AGCCCGAGCA GCTGATGGAC 
GCCAAACTGA AGTGCGTTTT CGAGATGCCC ACCGCTGAGG CAAATGCTGA GAACACCAGC 
GGTGGTGGTG CCGTTGGCGG CGGAACCGGA GCTGCCGGAG GCGGAAGCGC GGGTGCCAAT 
ACTAGCTCAG CCAGCGCTGA GGCGCTCGAG AGCAAGCCGA AGCTCTCCAG CGAGGATAAG 
TTTAAGCCAT CCAATTTGCT CGAAACGTCT GAGAGTCTGG ACTTGCTGTC CGGAGAGATC 
AAAGCGCTGC GTGAATGCAA CATTGAATTG CGAAGAGAGA ATCTTCACTT GAAGGATCAA 
ATCACACGTT TCCGGAGCTC GCCGGCCGTC AAACAGGTGA ATGAGCCCTA TGCCCCAGTC 
CTGGCTGAGA AGCAGATTCC GGTCTTTTAC ATTGCAGTTG CCATTGCTGC GGCCATCGTT 
AGCCTCCTGC TGGGCAAATT CTTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vap33 ( NM_206624.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996347.1 Drosophila melanogaster VAMP-associated protein 33kDa (Vap33), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00741
Clone ID Related Accession (Same CDS sequence)	NM_206623.2 , NM_130731.3 , NM_206624.2
Accession Version	NM_206624.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	VAMP-associated protein 33kDa, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206624.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAAAT CACTCTTTGA TCTTCCGTTG ACCATTGAAC CAGAACATGA GTTGCGTTTT 
GTGGGTCCCT TCACCCGACC CGTTGTCACA ATCATGACTC TGCGCAACAA CTCGGCTCTG 
CCTCTGGTCT TCAAGATCAA GACAACCGCC CCGAAACGCT ACTGCGTACG TCCAAACATC 
GGCAAGATAA TTCCCTTTCG ATCAACCCAG GTGGAGATCT GCCTTCAGCC ATTCGTCTAC 
GATCAGCAGG AGAAGAACAA GCACAAGTTC ATGGTGCAGA GCGTCCTGGC ACCCATGGAT 
GCTGATCTAA GCGATTTAAA TAAATTGTGG AAGGATCTGG AGCCCGAGCA GCTGATGGAC 
GCCAAACTGA AGTGCGTTTT CGAGATGCCC ACCGCTGAGG CAAATGCTGA GAACACCAGC 
GGTGGTGGTG CCGTTGGCGG CGGAACCGGA GCTGCCGGAG GCGGAAGCGC GGGTGCCAAT 
ACTAGCTCAG CCAGCGCTGA GGCGCTCGAG AGCAAGCCGA AGCTCTCCAG CGAGGATAAG 
TTTAAGCCAT CCAATTTGCT CGAAACGTCT GAGAGTCTGG ACTTGCTGTC CGGAGAGATC 
AAAGCGCTGC GTGAATGCAA CATTGAATTG CGAAGAGAGA ATCTTCACTT GAAGGATCAA 
ATCACACGTT TCCGGAGCTC GCCGGCCGTC AAACAGGTGA ATGAGCCCTA TGCCCCAGTC 
CTGGCTGAGA AGCAGATTCC GGTCTTTTAC ATTGCAGTTG CCATTGCTGC GGCCATCGTT 
AGCCTCCTGC TGGGCAAATT CTTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996347.1
CDS	278..1087
Translation	MSKSLFDLPLTIEPEHELRFVGPFTRPVVTIMTLRNNSALPLVFKIKTTAPKRYCVRPNIGKIIPFRSTQVEICLQPFVYDQQEKNKHKFMVQSVLAPMDADLSDLNKLWKDLEPEQLMDAKLKCVFEMPTAEANAENTSGGGAVGGGTGAAGGGSAGANTSSASAEALESKPKLSSEDKFKPSNLLETSESLDLLSGEIKALRECNIELRRENLHLKDQITRFRSSPAVKQVNEPYAPVLAEKQIPVFYIAVAIAAAIVSLLLGKFFL

Target ORF information:

RefSeq Version	NM_206624.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster VAMP-associated protein 33kDa (Vap33), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206624.2

ORF Insert Sequence:

ATGAGCAAAT CACTCTTTGA TCTTCCGTTG ACCATTGAAC CAGAACATGA GTTGCGTTTT 
GTGGGTCCCT TCACCCGACC CGTTGTCACA ATCATGACTC TGCGCAACAA CTCGGCTCTG 
CCTCTGGTCT TCAAGATCAA GACAACCGCC CCGAAACGCT ACTGCGTACG TCCAAACATC 
GGCAAGATAA TTCCCTTTCG ATCAACCCAG GTGGAGATCT GCCTTCAGCC ATTCGTCTAC 
GATCAGCAGG AGAAGAACAA GCACAAGTTC ATGGTGCAGA GCGTCCTGGC ACCCATGGAT 
GCTGATCTAA GCGATTTAAA TAAATTGTGG AAGGATCTGG AGCCCGAGCA GCTGATGGAC 
GCCAAACTGA AGTGCGTTTT CGAGATGCCC ACCGCTGAGG CAAATGCTGA GAACACCAGC 
GGTGGTGGTG CCGTTGGCGG CGGAACCGGA GCTGCCGGAG GCGGAAGCGC GGGTGCCAAT 
ACTAGCTCAG CCAGCGCTGA GGCGCTCGAG AGCAAGCCGA AGCTCTCCAG CGAGGATAAG 
TTTAAGCCAT CCAATTTGCT CGAAACGTCT GAGAGTCTGG ACTTGCTGTC CGGAGAGATC 
AAAGCGCTGC GTGAATGCAA CATTGAATTG CGAAGAGAGA ATCTTCACTT GAAGGATCAA 
ATCACACGTT TCCGGAGCTC GCCGGCCGTC AAACAGGTGA ATGAGCCCTA TGCCCCAGTC 
CTGGCTGAGA AGCAGATTCC GGTCTTTTAC ATTGCAGTTG CCATTGCTGC GGCCATCGTT 
AGCCTCCTGC TGGGCAAATT CTTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vap33 ( NM_206625.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996348.1 Drosophila melanogaster VAMP-associated protein 33kDa (Vap33), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00742
Clone ID Related Accession (Same CDS sequence)	NM_206625.2
Accession Version	NM_206625.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	VAMP-associated protein 33kDa, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206625.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAAAT CACTCTTTGA TCTTCCGTTG ACCATTGAAC CAGAACATGA GTTGCGTTTT 
GTGGGTCCCT TCACCCGACC CGTTGTCACA ATCATGACTC TGCGCAACAA CTCGGCTCTG 
CCTCTGGTCT TCAAGATCAA GACAACCGCC CCGAAACGCT ACTGCGTACG TCCAAACATC 
GGCAAGATAA TTCCCTTTCG ATCAACCCAG GTGGAGATCT GCCTTCAGCC ATTCGTCTAC 
GATCAGCAGG AGAAGAACAA GCACAAGTTC ATGGTGCAGA GCGTCCTGGC ACCCATGGAT 
GCTGATCTAA GCGATTTAAA TAAATTGTGG AAGGATCTGG AGCCCGAGCA GCTGATGGAC 
GCCAAACTGA AGTGCGTTTT CGAGATGCCC ACCGCTGAGG CGCTCGAGAG CAAGCCGAAG 
CTCTCCAGCG AGGATAAGTT TAAGCCATCC AATTTGCTCG AAACGTCTGA GAGTCTGGAC 
TTGCTGTCCG GAGAGATCAA AGCGCTGCGT GAATGCAACA TTGAATTGCG AAGAGAGAAT 
CTTCACTTGA AGGATCAAAT CACACGTTTC CGGAGCTCGC CGGCCGTCAA ACAGGTGAAT 
GAGCCCTATG CCCCAGTCCT GGCTGAGAAG CAGATTCCGG TCTTTTACAT TGCAGTTGCC 
ATTGCTGCGG CCATCGTTAG CCTCCTGCTG GGCAAATTCT TTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996348.1
CDS	568..1275
Translation	MSKSLFDLPLTIEPEHELRFVGPFTRPVVTIMTLRNNSALPLVFKIKTTAPKRYCVRPNIGKIIPFRSTQVEICLQPFVYDQQEKNKHKFMVQSVLAPMDADLSDLNKLWKDLEPEQLMDAKLKCVFEMPTAEALESKPKLSSEDKFKPSNLLETSESLDLLSGEIKALRECNIELRRENLHLKDQITRFRSSPAVKQVNEPYAPVLAEKQIPVFYIAVAIAAAIVSLLLGKFFL

Target ORF information:

RefSeq Version	NM_206625.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster VAMP-associated protein 33kDa (Vap33), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206625.2

ORF Insert Sequence:

ATGAGCAAAT CACTCTTTGA TCTTCCGTTG ACCATTGAAC CAGAACATGA GTTGCGTTTT 
GTGGGTCCCT TCACCCGACC CGTTGTCACA ATCATGACTC TGCGCAACAA CTCGGCTCTG 
CCTCTGGTCT TCAAGATCAA GACAACCGCC CCGAAACGCT ACTGCGTACG TCCAAACATC 
GGCAAGATAA TTCCCTTTCG ATCAACCCAG GTGGAGATCT GCCTTCAGCC ATTCGTCTAC 
GATCAGCAGG AGAAGAACAA GCACAAGTTC ATGGTGCAGA GCGTCCTGGC ACCCATGGAT 
GCTGATCTAA GCGATTTAAA TAAATTGTGG AAGGATCTGG AGCCCGAGCA GCTGATGGAC 
GCCAAACTGA AGTGCGTTTT CGAGATGCCC ACCGCTGAGG CGCTCGAGAG CAAGCCGAAG 
CTCTCCAGCG AGGATAAGTT TAAGCCATCC AATTTGCTCG AAACGTCTGA GAGTCTGGAC 
TTGCTGTCCG GAGAGATCAA AGCGCTGCGT GAATGCAACA TTGAATTGCG AAGAGAGAAT 
CTTCACTTGA AGGATCAAAT CACACGTTTC CGGAGCTCGC CGGCCGTCAA ACAGGTGAAT 
GAGCCCTATG CCCCAGTCCT GGCTGAGAAG CAGATTCCGG TCTTTTACAT TGCAGTTGCC 
ATTGCTGCGG CCATCGTTAG CCTCCTGCTG GGCAAATTCT TTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vap33 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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