Rala cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rala
Entrez Gene ID	31332
Full Name	Ras-like protein A
Synonyms	24639550,CG2849,D-RalA,DRal,DRala,Dm RalA,Dmel\CG2849,Dral,DralA,Drala,P48555,RALA,Ral,Ral-a,RalA,anon-EST:fe1H4,dRal,l(1)G0174,l(1)G0373,l(1)G0501,ral,ralA
General protein information	Preferred Names Ras-like protein A Names CG2849 gene product from transcript CG2849-RC CG2849-PA CG2849-PB CG2849-PC Rala-PA Rala-PB Rala-PC Ras-related protein anon-fast-evolving-1H4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3E5-3E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_166985.3	NP_726881.1	Ras-like protein A, isoform B
NM_166986.3	NP_726882.1	Ras-like protein A, isoform A
NM_080324.4	NP_525063.1	Ras-like protein A, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RALA	XP_001140368.1
Bos taurus (cattle)	RALA	NP_001029816.1
Drosophila melanogaster (fruit fly)	Rala	NP_726882.1
Xenopus tropicalis (tropical clawed frog)	rala	NP_001015915.1
Homo sapiens (human)	RALA	NP_005393.2
Caenorhabditis elegans (roundworm)	ral-1	NP_001254866.1
Mus musculus (house mouse)	Rala	NP_062364.3
Rattus norvegicus (Norway rat)	Rala	NP_112355.1
Gallus gallus (chicken)	RALA	XP_004939621.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012108	XP_320421.3
	RALA	XP_001099131.1
Canis lupus familiaris (dog)	RALA	XP_533083.2
Danio rerio (zebrafish)	ralaa	NP_957312.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data indicate that Ral and the exocyst regulate autophagy in a context-dependent manner, and that in dying salivary glands, Ral mediates autophagy, at least in part, by regulation of Notch.
Title: Ral GTPase and the exocyst regulate autophagy in a tissue-specific manner.

These studies identify a pathway, which stretches from Rab8 to RalA and the exocyst complex, that mediates rapid furrow formation in early Drosophila embryos.
Title: A rapid, membrane-dependent pathway directs furrow formation through RalA in the early Drosophila embryo.

Constitutively active Ral expressed in muscle causes the exocyst to be concentrated in the region surrounding synaptic boutons and consequently enlarges the membrane folds of the postsynaptic plasma membrane.
Title: Ral mediates activity-dependent growth of postsynaptic membranes via recruitment of the exocyst.

Ral inhibits ligandindependent Notch activation, all known forms of which take place in endosomes.
Title: Ral inhibits ligand-independent Notch signaling in Drosophila.

the Rap1-Rgl-Ral signaling network is a novel mechanism that cooperates with other intrinsic polarity cues to modulate asymmetric neuroblast division
Title: The Rap1-Rgl-Ral signaling network regulates neuroblast cortical polarity and spindle orientation.

The following Rala gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rala cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00722	NM_166985.3 Latest version!	Drosophila melanogaster Ras-like protein A (Rala), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa00723	NM_080324.4 Latest version!	Drosophila melanogaster Ras-like protein A (Rala), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00723	NM_166986.3 Latest version!	Drosophila melanogaster Ras-like protein A (Rala), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rala ( NM_166985.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726881.1 Drosophila melanogaster Ras-like protein A (Rala), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00722
Clone ID Related Accession (Same CDS sequence)	NM_166985.3
Accession Version	NM_166985.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ras-like protein A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_166985.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAAGA AGCCGACAGC CGGACCGGCG CTCCACAAGG TCATAATGGT GGGCAGTGGC 
GGCGTGGGAA AGTCCGCCCT CACACTGCAG TTTATGTACG ATGAGTTCGT CGAGGACTAC 
GAGCCCACCA AGGCCGATAG CTATAGGAAA AAGGTTGTGC TGGACGGCGA GGAAGTACAA 
ATAGATATAT TGGATACGGC TGGCCAGGAG GATTATGCCG CCATCAGAGA TAACTACTTT 
CGCAGCGGCG AGGGTTTCCT TTGCGTCTTC TCCATCACAG ACGACGAAAG CTTCCAGGCA 
ACCCAGGAAT TCAGAGAACA AATCTTGCGG GTGAAGAATG ATGAGAGCAT ACCGTTCCTG 
CTGGTGGGCA ACAAGTGCGA TCTGAATGAT AAGCGCAAGG TGCCGCTGAG CGAGTGCCAG 
CTGAGGGCGC AGCAGTGGGC GGTGCCCTAT GTGGAGACCT CGGCTAAAAC CCGCGAAAAT 
GTGGACAAGG TATTTTATGA TCTAATCAGG GATATTTCAT CGCGTAAAAA ACAACGTCAG 
ACGGACGTGA AACAGCCGCC GAAGCCCAAC TCTCATTGCT GCCAACTGCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726881.1
CDS	301..894
Translation	MSKKPTAGPALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITDDESFQATQEFREQILRVKNDESIPFLLVGNKCDLNDKRKVPLSECQLRAQQWAVPYVETSAKTRENVDKVFYDLIRDISSRKKQRQTDVKQPPKPNSHCCQLL

Target ORF information:

RefSeq Version	NM_166985.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ras-like protein A (Rala), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166985.3

ORF Insert Sequence:

ATGAGCAAGA AGCCGACAGC CGGACCGGCG CTCCACAAGG TCATAATGGT GGGCAGTGGC 
GGCGTGGGAA AGTCCGCCCT CACACTGCAG TTTATGTACG ATGAGTTCGT CGAGGACTAC 
GAGCCCACCA AGGCCGATAG CTATAGGAAA AAGGTTGTGC TGGACGGCGA GGAAGTACAA 
ATAGATATAT TGGATACGGC TGGCCAGGAG GATTATGCCG CCATCAGAGA TAACTACTTT 
CGCAGCGGCG AGGGTTTCCT TTGCGTCTTC TCCATCACAG ACGACGAAAG CTTCCAGGCA 
ACCCAGGAAT TCAGAGAACA AATCTTGCGG GTGAAGAATG ATGAGAGCAT ACCGTTCCTG 
CTGGTGGGCA ACAAGTGCGA TCTGAATGAT AAGCGCAAGG TGCCGCTGAG CGAGTGCCAG 
CTGAGGGCGC AGCAGTGGGC GGTGCCCTAT GTGGAGACCT CGGCTAAAAC CCGCGAAAAT 
GTGGACAAGG TATTTTATGA TCTAATCAGG GATATTTCAT CGCGTAAAAA ACAACGTCAG 
ACGGACGTGA AACAGCCGCC GAAGCCCAAC TCTCATTGCT GCCAACTGCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rala ( NM_166986.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726882.1 Drosophila melanogaster Ras-like protein A (Rala), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00723
Clone ID Related Accession (Same CDS sequence)	NM_166986.3 , NM_080324.4
Accession Version	NM_166986.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ras-like protein A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_166986.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAAGA AGCCGACAGC CGGACCGGCG CTCCACAAGG TCATAATGGT GGGCAGTGGC 
GGCGTGGGAA AGTCCGCCCT CACACTGCAG TTTATGTACG ATGAGTTCGT CGAGGACTAC 
GAGCCCACCA AGGCCGATAG CTATAGGAAA AAGGTTGTGC TGGACGGCGA GGAAGTACAA 
ATAGATATAT TGGATACGGC TGGCCAGGAG GATTATGCCG CCATCAGAGA TAACTACTTT 
CGCAGCGGCG AGGGTTTCCT TTGCGTCTTC TCCATCACAG ACGACGAAAG CTTCCAGGCA 
ACCCAGGAAT TCAGAGAACA AATCTTGCGG GTGAAGAATG ATGAGAGCAT ACCGTTCCTG 
CTGGTGGGCA ACAAGTGCGA TCTGAATGAT AAGCGCAAGG TGCCGCTGAG CGAGTGCCAG 
CTGAGGGCGC AGCAGTGGGC GGTGCCCTAT GTGGAGACCT CGGCTAAAAC CCGCGAAAAT 
GTGGACAAGG TATTTTTTGA TTTGATGCGC GAAATAAGAT CACGAAAAAC CGAAGATTCG 
AAAGCCACGA GCGGGCGTGC TAAAGATAGA TGCAAGAAGC GGAGACTTAA GTGTACCCTA 
CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726882.1
CDS	497..1102
Translation	MSKKPTAGPALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITDDESFQATQEFREQILRVKNDESIPFLLVGNKCDLNDKRKVPLSECQLRAQQWAVPYVETSAKTRENVDKVFFDLMREIRSRKTEDSKATSGRAKDRCKKRRLKCTLL

Target ORF information:

RefSeq Version	NM_166986.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ras-like protein A (Rala), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166986.3

ORF Insert Sequence:

ATGAGCAAGA AGCCGACAGC CGGACCGGCG CTCCACAAGG TCATAATGGT GGGCAGTGGC 
GGCGTGGGAA AGTCCGCCCT CACACTGCAG TTTATGTACG ATGAGTTCGT CGAGGACTAC 
GAGCCCACCA AGGCCGATAG CTATAGGAAA AAGGTTGTGC TGGACGGCGA GGAAGTACAA 
ATAGATATAT TGGATACGGC TGGCCAGGAG GATTATGCCG CCATCAGAGA TAACTACTTT 
CGCAGCGGCG AGGGTTTCCT TTGCGTCTTC TCCATCACAG ACGACGAAAG CTTCCAGGCA 
ACCCAGGAAT TCAGAGAACA AATCTTGCGG GTGAAGAATG ATGAGAGCAT ACCGTTCCTG 
CTGGTGGGCA ACAAGTGCGA TCTGAATGAT AAGCGCAAGG TGCCGCTGAG CGAGTGCCAG 
CTGAGGGCGC AGCAGTGGGC GGTGCCCTAT GTGGAGACCT CGGCTAAAAC CCGCGAAAAT 
GTGGACAAGG TATTTTTTGA TTTGATGCGC GAAATAAGAT CACGAAAAAC CGAAGATTCG 
AAAGCCACGA GCGGGCGTGC TAAAGATAGA TGCAAGAAGC GGAGACTTAA GTGTACCCTA 
CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rala ( NM_080324.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525063.1 Drosophila melanogaster Ras-like protein A (Rala), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00723
Clone ID Related Accession (Same CDS sequence)	NM_166986.3 , NM_080324.4
Accession Version	NM_080324.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ras-like protein A, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080324.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAAGA AGCCGACAGC CGGACCGGCG CTCCACAAGG TCATAATGGT GGGCAGTGGC 
GGCGTGGGAA AGTCCGCCCT CACACTGCAG TTTATGTACG ATGAGTTCGT CGAGGACTAC 
GAGCCCACCA AGGCCGATAG CTATAGGAAA AAGGTTGTGC TGGACGGCGA GGAAGTACAA 
ATAGATATAT TGGATACGGC TGGCCAGGAG GATTATGCCG CCATCAGAGA TAACTACTTT 
CGCAGCGGCG AGGGTTTCCT TTGCGTCTTC TCCATCACAG ACGACGAAAG CTTCCAGGCA 
ACCCAGGAAT TCAGAGAACA AATCTTGCGG GTGAAGAATG ATGAGAGCAT ACCGTTCCTG 
CTGGTGGGCA ACAAGTGCGA TCTGAATGAT AAGCGCAAGG TGCCGCTGAG CGAGTGCCAG 
CTGAGGGCGC AGCAGTGGGC GGTGCCCTAT GTGGAGACCT CGGCTAAAAC CCGCGAAAAT 
GTGGACAAGG TATTTTTTGA TTTGATGCGC GAAATAAGAT CACGAAAAAC CGAAGATTCG 
AAAGCCACGA GCGGGCGTGC TAAAGATAGA TGCAAGAAGC GGAGACTTAA GTGTACCCTA 
CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525063.1
CDS	301..906
Translation	MSKKPTAGPALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITDDESFQATQEFREQILRVKNDESIPFLLVGNKCDLNDKRKVPLSECQLRAQQWAVPYVETSAKTRENVDKVFFDLMREIRSRKTEDSKATSGRAKDRCKKRRLKCTLL

Target ORF information:

RefSeq Version	NM_080324.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ras-like protein A (Rala), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080324.4

ORF Insert Sequence:

ATGAGCAAGA AGCCGACAGC CGGACCGGCG CTCCACAAGG TCATAATGGT GGGCAGTGGC 
GGCGTGGGAA AGTCCGCCCT CACACTGCAG TTTATGTACG ATGAGTTCGT CGAGGACTAC 
GAGCCCACCA AGGCCGATAG CTATAGGAAA AAGGTTGTGC TGGACGGCGA GGAAGTACAA 
ATAGATATAT TGGATACGGC TGGCCAGGAG GATTATGCCG CCATCAGAGA TAACTACTTT 
CGCAGCGGCG AGGGTTTCCT TTGCGTCTTC TCCATCACAG ACGACGAAAG CTTCCAGGCA 
ACCCAGGAAT TCAGAGAACA AATCTTGCGG GTGAAGAATG ATGAGAGCAT ACCGTTCCTG 
CTGGTGGGCA ACAAGTGCGA TCTGAATGAT AAGCGCAAGG TGCCGCTGAG CGAGTGCCAG 
CTGAGGGCGC AGCAGTGGGC GGTGCCCTAT GTGGAGACCT CGGCTAAAAC CCGCGAAAAT 
GTGGACAAGG TATTTTTTGA TTTGATGCGC GAAATAAGAT CACGAAAAAC CGAAGATTCG 
AAAGCCACGA GCGGGCGTGC TAAAGATAGA TGCAAGAAGC GGAGACTTAA GTGTACCCTA 
CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rala cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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