CG12535 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12535
Entrez Gene ID	31311
Full Name	CG12535 gene product from transcript CG12535-RF
Synonyms	Dmel\CG12535
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3D2-3D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_166972.2	NP_726860.1	uncharacterized protein, isoform B
NM_001297909.1	NP_001284838.1	uncharacterized protein, isoform F
NM_001272277.1	NP_001259206.1	uncharacterized protein, isoform E
NM_001272276.1	NP_001259205.1	uncharacterized protein, isoform C
NM_130699.2	NP_570055.2	uncharacterized protein, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG12535 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12535 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00701	NM_166972.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12535), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00701	NM_001272276.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG12535), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00701	NM_001272277.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant E (CG12535), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00701	NM_130699.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG12535), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00701	NM_001297909.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant F (CG12535), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12535 ( NM_166972.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726860.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG12535), mRNA.

Price & Availability↑

CloneID	OFa00701
Clone ID Related Accession (Same CDS sequence)	NM_166972.2 , NM_001297909.1 , NM_001272277.1 , NM_001272276.1 , NM_130699.2
Accession Version	NM_166972.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_166972.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGAAC TGCAGCATAT TACCACTCGG ATTCGCACAC TTTTGCGTAG AACCTTTCAC 
CTGTCCAGCA ATTATGACAC GTATTCGATT TGGACCTTTT GCCCGATGAC CAATTGGAAC 
CAGCTTAAAC CCGTGCTCCA ATCCTGCAAT CTGTGCCGCG TCGAATCATT TCCCATTGTC 
TTTTGGCTGG TGGTGCTGCT CAGTGTCTTT GGAACATGTT CCAATTGCTA TGAACTGATG 
AAAGTTTGGT CATGTCCCTG CACCAAATTG GCCATGTGGC GCCAACGGCA CTTCTATGTC 
CTAAAACATT GGACGCTGCG AAAGGCTCGA CTTTTGAGCT TGGCCATAAT CCTGATCGCT 
TGGCTAATGC TTATTTACGG AATCGTAACG ATCTCCCCCT ACGCCATGTC GCCATGGATT 
CTGGTGACCA GCATCGTCCT AACGATGGAG TGCTTCCTTT GGTCCTTCGA CGTATTGACC 
GGCCGTTTGC CGCTCGATCC GCAGACGCTG CTGTCGCTCG TTTTGCATGT GATCTTCGTC 
GCGATGGTTT GCTGCGTGAA GAGCGTTTTC GAGTTGGCGC TCGGCGAGCA CGTCGAGCAC 
TCGCTGCGTA TCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726860.1
CDS	597..1214
Translation	MFELQHITTRIRTLLRRTFHLSSNYDTYSIWTFCPMTNWNQLKPVLQSCNLCRVESFPIVFWLVVLLSVFGTCSNCYELMKVWSCPCTKLAMWRQRHFYVLKHWTLRKARLLSLAIILIAWLMLIYGIVTISPYAMSPWILVTSIVLTMECFLWSFDVLTGRLPLDPQTLLSLVLHVIFVAMVCCVKSVFELALGEHVEHSLRII

Target ORF information:

RefSeq Version	NM_166972.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12535), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166972.2

ORF Insert Sequence:

ATGTTCGAAC TGCAGCATAT TACCACTCGG ATTCGCACAC TTTTGCGTAG AACCTTTCAC 
CTGTCCAGCA ATTATGACAC GTATTCGATT TGGACCTTTT GCCCGATGAC CAATTGGAAC 
CAGCTTAAAC CCGTGCTCCA ATCCTGCAAT CTGTGCCGCG TCGAATCATT TCCCATTGTC 
TTTTGGCTGG TGGTGCTGCT CAGTGTCTTT GGAACATGTT CCAATTGCTA TGAACTGATG 
AAAGTTTGGT CATGTCCCTG CACCAAATTG GCCATGTGGC GCCAACGGCA CTTCTATGTC 
CTAAAACATT GGACGCTGCG AAAGGCTCGA CTTTTGAGCT TGGCCATAAT CCTGATCGCT 
TGGCTAATGC TTATTTACGG AATCGTAACG ATCTCCCCCT ACGCCATGTC GCCATGGATT 
CTGGTGACCA GCATCGTCCT AACGATGGAG TGCTTCCTTT GGTCCTTCGA CGTATTGACC 
GGCCGTTTGC CGCTCGATCC GCAGACGCTG CTGTCGCTCG TTTTGCATGT GATCTTCGTC 
GCGATGGTTT GCTGCGTGAA GAGCGTTTTC GAGTTGGCGC TCGGCGAGCA CGTCGAGCAC 
TCGCTGCGTA TCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12535 ( NM_001297909.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284838.1 Drosophila melanogaster uncharacterized protein, transcript variant F (CG12535), mRNA.

Price & Availability↑

CloneID	OFa00701
Clone ID Related Accession (Same CDS sequence)	NM_166972.2 , NM_001297909.1 , NM_001272277.1 , NM_001272276.1 , NM_130699.2
Accession Version	NM_001297909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGAAC TGCAGCATAT TACCACTCGG ATTCGCACAC TTTTGCGTAG AACCTTTCAC 
CTGTCCAGCA ATTATGACAC GTATTCGATT TGGACCTTTT GCCCGATGAC CAATTGGAAC 
CAGCTTAAAC CCGTGCTCCA ATCCTGCAAT CTGTGCCGCG TCGAATCATT TCCCATTGTC 
TTTTGGCTGG TGGTGCTGCT CAGTGTCTTT GGAACATGTT CCAATTGCTA TGAACTGATG 
AAAGTTTGGT CATGTCCCTG CACCAAATTG GCCATGTGGC GCCAACGGCA CTTCTATGTC 
CTAAAACATT GGACGCTGCG AAAGGCTCGA CTTTTGAGCT TGGCCATAAT CCTGATCGCT 
TGGCTAATGC TTATTTACGG AATCGTAACG ATCTCCCCCT ACGCCATGTC GCCATGGATT 
CTGGTGACCA GCATCGTCCT AACGATGGAG TGCTTCCTTT GGTCCTTCGA CGTATTGACC 
GGCCGTTTGC CGCTCGATCC GCAGACGCTG CTGTCGCTCG TTTTGCATGT GATCTTCGTC 
GCGATGGTTT GCTGCGTGAA GAGCGTTTTC GAGTTGGCGC TCGGCGAGCA CGTCGAGCAC 
TCGCTGCGTA TCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284838.1
CDS	597..1214
Translation	MFELQHITTRIRTLLRRTFHLSSNYDTYSIWTFCPMTNWNQLKPVLQSCNLCRVESFPIVFWLVVLLSVFGTCSNCYELMKVWSCPCTKLAMWRQRHFYVLKHWTLRKARLLSLAIILIAWLMLIYGIVTISPYAMSPWILVTSIVLTMECFLWSFDVLTGRLPLDPQTLLSLVLHVIFVAMVCCVKSVFELALGEHVEHSLRII

Target ORF information:

RefSeq Version	NM_001297909.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant F (CG12535), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297909.1

ORF Insert Sequence:

ATGTTCGAAC TGCAGCATAT TACCACTCGG ATTCGCACAC TTTTGCGTAG AACCTTTCAC 
CTGTCCAGCA ATTATGACAC GTATTCGATT TGGACCTTTT GCCCGATGAC CAATTGGAAC 
CAGCTTAAAC CCGTGCTCCA ATCCTGCAAT CTGTGCCGCG TCGAATCATT TCCCATTGTC 
TTTTGGCTGG TGGTGCTGCT CAGTGTCTTT GGAACATGTT CCAATTGCTA TGAACTGATG 
AAAGTTTGGT CATGTCCCTG CACCAAATTG GCCATGTGGC GCCAACGGCA CTTCTATGTC 
CTAAAACATT GGACGCTGCG AAAGGCTCGA CTTTTGAGCT TGGCCATAAT CCTGATCGCT 
TGGCTAATGC TTATTTACGG AATCGTAACG ATCTCCCCCT ACGCCATGTC GCCATGGATT 
CTGGTGACCA GCATCGTCCT AACGATGGAG TGCTTCCTTT GGTCCTTCGA CGTATTGACC 
GGCCGTTTGC CGCTCGATCC GCAGACGCTG CTGTCGCTCG TTTTGCATGT GATCTTCGTC 
GCGATGGTTT GCTGCGTGAA GAGCGTTTTC GAGTTGGCGC TCGGCGAGCA CGTCGAGCAC 
TCGCTGCGTA TCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12535 ( NM_001272277.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259206.1 Drosophila melanogaster uncharacterized protein, transcript variant E (CG12535), mRNA.

Price & Availability↑

CloneID	OFa00701
Clone ID Related Accession (Same CDS sequence)	NM_166972.2 , NM_001297909.1 , NM_001272277.1 , NM_001272276.1 , NM_130699.2
Accession Version	NM_001272277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGAAC TGCAGCATAT TACCACTCGG ATTCGCACAC TTTTGCGTAG AACCTTTCAC 
CTGTCCAGCA ATTATGACAC GTATTCGATT TGGACCTTTT GCCCGATGAC CAATTGGAAC 
CAGCTTAAAC CCGTGCTCCA ATCCTGCAAT CTGTGCCGCG TCGAATCATT TCCCATTGTC 
TTTTGGCTGG TGGTGCTGCT CAGTGTCTTT GGAACATGTT CCAATTGCTA TGAACTGATG 
AAAGTTTGGT CATGTCCCTG CACCAAATTG GCCATGTGGC GCCAACGGCA CTTCTATGTC 
CTAAAACATT GGACGCTGCG AAAGGCTCGA CTTTTGAGCT TGGCCATAAT CCTGATCGCT 
TGGCTAATGC TTATTTACGG AATCGTAACG ATCTCCCCCT ACGCCATGTC GCCATGGATT 
CTGGTGACCA GCATCGTCCT AACGATGGAG TGCTTCCTTT GGTCCTTCGA CGTATTGACC 
GGCCGTTTGC CGCTCGATCC GCAGACGCTG CTGTCGCTCG TTTTGCATGT GATCTTCGTC 
GCGATGGTTT GCTGCGTGAA GAGCGTTTTC GAGTTGGCGC TCGGCGAGCA CGTCGAGCAC 
TCGCTGCGTA TCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259206.1
CDS	848..1465
Translation	MFELQHITTRIRTLLRRTFHLSSNYDTYSIWTFCPMTNWNQLKPVLQSCNLCRVESFPIVFWLVVLLSVFGTCSNCYELMKVWSCPCTKLAMWRQRHFYVLKHWTLRKARLLSLAIILIAWLMLIYGIVTISPYAMSPWILVTSIVLTMECFLWSFDVLTGRLPLDPQTLLSLVLHVIFVAMVCCVKSVFELALGEHVEHSLRII

Target ORF information:

RefSeq Version	NM_001272277.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant E (CG12535), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272277.1

ORF Insert Sequence:

ATGTTCGAAC TGCAGCATAT TACCACTCGG ATTCGCACAC TTTTGCGTAG AACCTTTCAC 
CTGTCCAGCA ATTATGACAC GTATTCGATT TGGACCTTTT GCCCGATGAC CAATTGGAAC 
CAGCTTAAAC CCGTGCTCCA ATCCTGCAAT CTGTGCCGCG TCGAATCATT TCCCATTGTC 
TTTTGGCTGG TGGTGCTGCT CAGTGTCTTT GGAACATGTT CCAATTGCTA TGAACTGATG 
AAAGTTTGGT CATGTCCCTG CACCAAATTG GCCATGTGGC GCCAACGGCA CTTCTATGTC 
CTAAAACATT GGACGCTGCG AAAGGCTCGA CTTTTGAGCT TGGCCATAAT CCTGATCGCT 
TGGCTAATGC TTATTTACGG AATCGTAACG ATCTCCCCCT ACGCCATGTC GCCATGGATT 
CTGGTGACCA GCATCGTCCT AACGATGGAG TGCTTCCTTT GGTCCTTCGA CGTATTGACC 
GGCCGTTTGC CGCTCGATCC GCAGACGCTG CTGTCGCTCG TTTTGCATGT GATCTTCGTC 
GCGATGGTTT GCTGCGTGAA GAGCGTTTTC GAGTTGGCGC TCGGCGAGCA CGTCGAGCAC 
TCGCTGCGTA TCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12535 ( NM_001272276.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259205.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG12535), mRNA.

Price & Availability↑

CloneID	OFa00701
Clone ID Related Accession (Same CDS sequence)	NM_166972.2 , NM_001297909.1 , NM_001272277.1 , NM_001272276.1 , NM_130699.2
Accession Version	NM_001272276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGAAC TGCAGCATAT TACCACTCGG ATTCGCACAC TTTTGCGTAG AACCTTTCAC 
CTGTCCAGCA ATTATGACAC GTATTCGATT TGGACCTTTT GCCCGATGAC CAATTGGAAC 
CAGCTTAAAC CCGTGCTCCA ATCCTGCAAT CTGTGCCGCG TCGAATCATT TCCCATTGTC 
TTTTGGCTGG TGGTGCTGCT CAGTGTCTTT GGAACATGTT CCAATTGCTA TGAACTGATG 
AAAGTTTGGT CATGTCCCTG CACCAAATTG GCCATGTGGC GCCAACGGCA CTTCTATGTC 
CTAAAACATT GGACGCTGCG AAAGGCTCGA CTTTTGAGCT TGGCCATAAT CCTGATCGCT 
TGGCTAATGC TTATTTACGG AATCGTAACG ATCTCCCCCT ACGCCATGTC GCCATGGATT 
CTGGTGACCA GCATCGTCCT AACGATGGAG TGCTTCCTTT GGTCCTTCGA CGTATTGACC 
GGCCGTTTGC CGCTCGATCC GCAGACGCTG CTGTCGCTCG TTTTGCATGT GATCTTCGTC 
GCGATGGTTT GCTGCGTGAA GAGCGTTTTC GAGTTGGCGC TCGGCGAGCA CGTCGAGCAC 
TCGCTGCGTA TCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259205.1
CDS	1029..1646
Translation	MFELQHITTRIRTLLRRTFHLSSNYDTYSIWTFCPMTNWNQLKPVLQSCNLCRVESFPIVFWLVVLLSVFGTCSNCYELMKVWSCPCTKLAMWRQRHFYVLKHWTLRKARLLSLAIILIAWLMLIYGIVTISPYAMSPWILVTSIVLTMECFLWSFDVLTGRLPLDPQTLLSLVLHVIFVAMVCCVKSVFELALGEHVEHSLRII

Target ORF information:

RefSeq Version	NM_001272276.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG12535), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272276.1

ORF Insert Sequence:

ATGTTCGAAC TGCAGCATAT TACCACTCGG ATTCGCACAC TTTTGCGTAG AACCTTTCAC 
CTGTCCAGCA ATTATGACAC GTATTCGATT TGGACCTTTT GCCCGATGAC CAATTGGAAC 
CAGCTTAAAC CCGTGCTCCA ATCCTGCAAT CTGTGCCGCG TCGAATCATT TCCCATTGTC 
TTTTGGCTGG TGGTGCTGCT CAGTGTCTTT GGAACATGTT CCAATTGCTA TGAACTGATG 
AAAGTTTGGT CATGTCCCTG CACCAAATTG GCCATGTGGC GCCAACGGCA CTTCTATGTC 
CTAAAACATT GGACGCTGCG AAAGGCTCGA CTTTTGAGCT TGGCCATAAT CCTGATCGCT 
TGGCTAATGC TTATTTACGG AATCGTAACG ATCTCCCCCT ACGCCATGTC GCCATGGATT 
CTGGTGACCA GCATCGTCCT AACGATGGAG TGCTTCCTTT GGTCCTTCGA CGTATTGACC 
GGCCGTTTGC CGCTCGATCC GCAGACGCTG CTGTCGCTCG TTTTGCATGT GATCTTCGTC 
GCGATGGTTT GCTGCGTGAA GAGCGTTTTC GAGTTGGCGC TCGGCGAGCA CGTCGAGCAC 
TCGCTGCGTA TCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12535 ( NM_130699.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_570055.2 Drosophila melanogaster uncharacterized protein, transcript variant D (CG12535), mRNA.

Price & Availability↑

CloneID	OFa00701
Clone ID Related Accession (Same CDS sequence)	NM_166972.2 , NM_001297909.1 , NM_001272277.1 , NM_001272276.1 , NM_130699.2
Accession Version	NM_130699.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_130699.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGAAC TGCAGCATAT TACCACTCGG ATTCGCACAC TTTTGCGTAG AACCTTTCAC 
CTGTCCAGCA ATTATGACAC GTATTCGATT TGGACCTTTT GCCCGATGAC CAATTGGAAC 
CAGCTTAAAC CCGTGCTCCA ATCCTGCAAT CTGTGCCGCG TCGAATCATT TCCCATTGTC 
TTTTGGCTGG TGGTGCTGCT CAGTGTCTTT GGAACATGTT CCAATTGCTA TGAACTGATG 
AAAGTTTGGT CATGTCCCTG CACCAAATTG GCCATGTGGC GCCAACGGCA CTTCTATGTC 
CTAAAACATT GGACGCTGCG AAAGGCTCGA CTTTTGAGCT TGGCCATAAT CCTGATCGCT 
TGGCTAATGC TTATTTACGG AATCGTAACG ATCTCCCCCT ACGCCATGTC GCCATGGATT 
CTGGTGACCA GCATCGTCCT AACGATGGAG TGCTTCCTTT GGTCCTTCGA CGTATTGACC 
GGCCGTTTGC CGCTCGATCC GCAGACGCTG CTGTCGCTCG TTTTGCATGT GATCTTCGTC 
GCGATGGTTT GCTGCGTGAA GAGCGTTTTC GAGTTGGCGC TCGGCGAGCA CGTCGAGCAC 
TCGCTGCGTA TCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570055.2
CDS	719..1336
Translation	MFELQHITTRIRTLLRRTFHLSSNYDTYSIWTFCPMTNWNQLKPVLQSCNLCRVESFPIVFWLVVLLSVFGTCSNCYELMKVWSCPCTKLAMWRQRHFYVLKHWTLRKARLLSLAIILIAWLMLIYGIVTISPYAMSPWILVTSIVLTMECFLWSFDVLTGRLPLDPQTLLSLVLHVIFVAMVCCVKSVFELALGEHVEHSLRII

Target ORF information:

RefSeq Version	NM_130699.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG12535), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130699.2

ORF Insert Sequence:

ATGTTCGAAC TGCAGCATAT TACCACTCGG ATTCGCACAC TTTTGCGTAG AACCTTTCAC 
CTGTCCAGCA ATTATGACAC GTATTCGATT TGGACCTTTT GCCCGATGAC CAATTGGAAC 
CAGCTTAAAC CCGTGCTCCA ATCCTGCAAT CTGTGCCGCG TCGAATCATT TCCCATTGTC 
TTTTGGCTGG TGGTGCTGCT CAGTGTCTTT GGAACATGTT CCAATTGCTA TGAACTGATG 
AAAGTTTGGT CATGTCCCTG CACCAAATTG GCCATGTGGC GCCAACGGCA CTTCTATGTC 
CTAAAACATT GGACGCTGCG AAAGGCTCGA CTTTTGAGCT TGGCCATAAT CCTGATCGCT 
TGGCTAATGC TTATTTACGG AATCGTAACG ATCTCCCCCT ACGCCATGTC GCCATGGATT 
CTGGTGACCA GCATCGTCCT AACGATGGAG TGCTTCCTTT GGTCCTTCGA CGTATTGACC 
GGCCGTTTGC CGCTCGATCC GCAGACGCTG CTGTCGCTCG TTTTGCATGT GATCTTCGTC 
GCGATGGTTT GCTGCGTGAA GAGCGTTTTC GAGTTGGCGC TCGGCGAGCA CGTCGAGCAC 
TCGCTGCGTA TCATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG12535 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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