crm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	crm
Entrez Gene ID	31268
Full Name	cramped
Synonyms	CG2714,Dmel\CG2714,EG:95B7.2,ZW-9,l(1)3C1,l(1)3Ca,l(1)zw9,sa,sta[P],swa,zw-9,zw9
General protein information	Preferred Names cramped Names CG2714 gene product from transcript CG2714-RB CG2714-PA CG2714-PB crm-PA crm-PB sparse-arista stubarista from P32 swollen-antenna
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3B3-3B4\|1-1.48 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_166951.2	NP_726829.1	cramped, isoform B
NM_057757.4	NP_477105.2	cramped, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CRM is specifically required for the expression of the Histone H1 gene.
Title: The Polycomb group protein CRAMPED is involved with TRF2 in the activation of the histone H1 gene.

crm modulates the effect of temperature on a genomic region encoding Hedgehog and Wingless signaling effectors.
Title: Segregating variation in the polycomb group gene cramped alters the effect of temperature on multiple traits.

The following crm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00650	NM_166951.2 Latest version!	Drosophila melanogaster cramped (crm), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00650	NM_057757.4 Latest version!	Drosophila melanogaster cramped (crm), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crm ( NM_166951.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726829.1 Drosophila melanogaster cramped (crm), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00650
Clone ID Related Accession (Same CDS sequence)	NM_166951.2 , NM_057757.4
Accession Version	NM_166951.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cramped, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_166951.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAC TGTCCAAGCA GCCGCCGCCA CCCCCGCTGA CGCAGCCGCC GCCGCCGTCG 
TCATCCGTGT CCATTGAGGA GCCGCTGCCA AATGGCAAGG GAGGAGGAGC AGTAGTTGTT 
AACTCGATAG CTAAGCTGCC CGAGGAGGAG CTCCTCGGCT CGGTGACCAT GCACAATTGT 
CCGGGAACGC GGGCCAGTGC CCGGGTCATA CAGAAGATGA AGCAGGACCA GACGCGCCCG 
ATGACACCGC CGCCCAGCGA ACGGGAGCCG AACAAAAAGG AGGAGAAGGC CGCCCAAAAG 
ACGCCCAGCC AGCTGAAGAC GGGCAGCGGG AAGACCACCT GGACGAATGT GGAGCGCAAC 
TGCTTCTTCG ACGCCCTCAA CGAATTCGGC AAGGACTTCG AGGCGGTGGC CAACTGCATC 
AATGCCAAGC TGAAGCGCCG CAATGCCAAC AGCGACTATA GTTTCAAAAC CAAGGACCAA 
GTTCGCCAGC ACTATTACCA GACCCATCAC AAGATTTGCA AATATGTGCG CTTCTCGGAG 
GAACTGAAAA AGCCCGCCCA GGAGTTGTAC ACGCTGATCA ACTACGGCGA GATGCGGCGC 
AAGCTGCAGT TCCTCACGGA GAAGCACTTC ATGAAGCTGA AGCAGCTGGT CTACCAGGGC 
CAAATAACCG TGCGTTGCAA GGGCAAGAAC ATTCGCATTA AGACGCCATC GTGCAAGGCG 
CTCCGGCGGC TAAATCAATT GGATGACTCA CTGGAGGATA TCCGGCTGCC CAGCAAGGTG 
GAGGTGCTGG TGACTCCCGC CAATATGGAG GCCTTCGGAC GCGTCCAGTC ACTGGCGCAG 
AATCCACGCG GCAGGATCAT AGTTCCGCTG CACAAGAAAC TAATCAGCTT CATCAAGACG 
TTCGAGTACA AATGGCGCAG CGCCAATCAG CGTTTGCACG AGGAGAAGAG TGCATACTTT 
TCCAGCAGCC TGCCATCTGC TGCGTCCAAC AACAATAACA ACAACAATGA AACGGAACCA 
TTACAGCCGT CAGTTGCATC GCTGGATCCC TCAATGTGCT TCCAGCCCCG GCCGGGAGTG 
GCCATCCACC GACCTTTGCT CAGCATCACC GCCTACCTAA GCAGCATCAG TATTTGCTTG 
ACGGCATACG AGGAGCGCAT GGGCTTCAAA GTGCGCAGCG AGACCTTGGG CAATTTGGCC 
GGCATGTCGG TGGCGGCCAA CAAGCGACTA CGAACGGAAA GCGGCTCCGA GAAGCGTTCG 
CCGGAGACAA AGAAACCCAA ATCGAGTGCC AGCCCGCCGC TGGAGAAGAG TTTGGACGAC 
GGGCCCTTGG AGGGCAATCT GATGAAGATG GAGAATAGCA GCGGTGATGA GCTGGGCGAG 
GAGATTCATG AGTTTCTGGG CGATATATTG GAGGCCATGC AGCATCCGCA AGCAGCCACT 
ATTCCAGCTC TTTCGGCCAC CACTGGTGAT ACCACAACTG TTGCTGTTGC GCTTGAAACA 
TCGCACGATC CTGTACAACA GGCTTATCCG GCCAACGCGG ATCTATCTAA TGCCATGGCG 
ACATCTGTTC TACAAACTTC CTGTGCTGCA GCTCCCGCTC CTTCGACTCC TGTAACCGGT 
TCTTTGGCGG CTCCATCCGT AGCCCGCTCC AAACGCAAAG AAGCTAAGGA GGCGGCGGCC 
GCGGCACAGG CTCGAAATTT TAAGCCACTG CTCAGCGACG ATATACTGAA GAGAATCCGC 
AAGGGCTGGA CGCAGGCGAA TGCGGCGGAT ATAACCATCG GTGATCTGTA TGTCGTTTTT 
GGCCAGGATT CCAAGCTGGA GCTGGAGTAT TATTGGTGTG AGGTCGACAG TTCGACGGCC 
ATGGCATCCA GCATACTAAC AATCAACACG GTAACGCCTA GCTCCTCGTC GGTGGCCACC 
CAAACCGGCA GCGCCGCCTC GAACGCCAAC CAAACAGGCG CATCTTCCAA CTGCTACGTC 
AGCGCATCGA GCAATTCGAG CACCTCGAGC ACTTCGCTGC CCTACAATCC CAACGACTGT 
GATAGCGTGG AACGCGTCAG GGCCGTCACC ACATCGTCGG TGAGCAACAA GCTGAAGCAC 
CTGCTACTCG TGGCCAATCT CAGCGAGCGC GTGCGCAAGC GCCAATGCAA CTGCGGCCAC 
ACGTGCGATC GCAAGCGGGA CCTGATGACC AAGGCGCAGC AGCTGGCAGA GGCGACGGCC 
ACCGGCGGAG TGGGCGGCAT GGTTGAGGGC AATTTCCGAA CGCCGATGCT GCCAGTGCGG 
CGGCCGATAT CCAACATCGA TCCTGTGCGC CAACTCTCAG CGCTCACACG GCAAAAGATC 
AGTCGTCAGG TGCTTGTGCA GCGCCGTCTC CTACCACCCA CATCCGCTGG CGATCGTCCG 
TACGATCTGC TGAGTGTGCG CCAACTCCAC AGTGGCCTTT TCGAGCCGAT TGATCGCGTG 
GATGGCATAA GTTCCGCTGG CATCAGCACC TCTGGCTCGA AACCCGATTC CAGCATGAAT 
GCCACGACCG CTTCACAGGA TCAAGAGCCA GGGGATCAGG GGGCATTGGA GTTCCTCAAC 
GACGAGGCCA CGCAGGTCAG CGCTCGAGAC ATGCCCAATC TGGACATTTG CGTGGCCACC 
AACAGGACGG ACGTCAGCAG TTCCCTGAAC GAGGCGGCCC AAGACGGGAG CACAACTCAA 
AGCTTCTTCC AAGGCAGCAT GAGTCCGATG CACTTGCTCA GAGATTCAAC ATCCAACGCA 
CGCTGGCTGG AGGACAATAT CAATGACTTC TCGTTGACGT CGCTGCTGGG GCATTTGGAT 
GAGATTGATG CCACCAGGGA TATACTGGAT CCGTCGTCAA GCATGTCGGT GATCAGCGAG 
AGCAGCGTGG ACTTTCGGCA CAAGTTCCAG GAAATCGCCG CCCTGCTGCA GCAGCAGGAG 
AAAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726829.1
CDS	134..3082
Translation	MEELSKQPPPPPLTQPPPPSSSVSIEEPLPNGKGGGAVVVNSIAKLPEEELLGSVTMHNCPGTRASARVIQKMKQDQTRPMTPPPSEREPNKKEEKAAQKTPSQLKTGSGKTTWTNVERNCFFDALNEFGKDFEAVANCINAKLKRRNANSDYSFKTKDQVRQHYYQTHHKICKYVRFSEELKKPAQELYTLINYGEMRRKLQFLTEKHFMKLKQLVYQGQITVRCKGKNIRIKTPSCKALRRLNQLDDSLEDIRLPSKVEVLVTPANMEAFGRVQSLAQNPRGRIIVPLHKKLISFIKTFEYKWRSANQRLHEEKSAYFSSSLPSAASNNNNNNNETEPLQPSVASLDPSMCFQPRPGVAIHRPLLSITAYLSSISICLTAYEERMGFKVRSETLGNLAGMSVAANKRLRTESGSEKRSPETKKPKSSASPPLEKSLDDGPLEGNLMKMENSSGDELGEEIHEFLGDILEAMQHPQAATIPALSATTGDTTTVAVALETSHDPVQQAYPANADLSNAMATSVLQTSCAAAPAPSTPVTGSLAAPSVARSKRKEAKEAAAAAQARNFKPLLSDDILKRIRKGWTQANAADITIGDLYVVFGQDSKLELEYYWCEVDSSTAMASSILTINTVTPSSSSVATQTGSAASNANQTGASSNCYVSASSNSSTSSTSLPYNPNDCDSVERVRAVTTSSVSNKLKHLLLVANLSERVRKRQCNCGHTCDRKRDLMTKAQQLAEATATGGVGGMVEGNFRTPMLPVRRPISNIDPVRQLSALTRQKISRQVLVQRRLLPPTSAGDRPYDLLSVRQLHSGLFEPIDRVDGISSAGISTSGSKPDSSMNATTASQDQEPGDQGALEFLNDEATQVSARDMPNLDICVATNRTDVSSSLNEAAQDGSTTQSFFQGSMSPMHLLRDSTSNARWLEDNINDFSLTSLLGHLDEIDATRDILDPSSSMSVISESSVDFRHKFQEIAALLQQQEKD

Target ORF information:

RefSeq Version	NM_166951.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cramped (crm), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166951.2

ORF Insert Sequence:

ATGGAGGAAC TGTCCAAGCA GCCGCCGCCA CCCCCGCTGA CGCAGCCGCC GCCGCCGTCG 
TCATCCGTGT CCATTGAGGA GCCGCTGCCA AATGGCAAGG GAGGAGGAGC AGTAGTTGTT 
AACTCGATAG CTAAGCTGCC CGAGGAGGAG CTCCTCGGCT CGGTGACCAT GCACAATTGT 
CCGGGAACGC GGGCCAGTGC CCGGGTCATA CAGAAGATGA AGCAGGACCA GACGCGCCCG 
ATGACACCGC CGCCCAGCGA ACGGGAGCCG AACAAAAAGG AGGAGAAGGC CGCCCAAAAG 
ACGCCCAGCC AGCTGAAGAC GGGCAGCGGG AAGACCACCT GGACGAATGT GGAGCGCAAC 
TGCTTCTTCG ACGCCCTCAA CGAATTCGGC AAGGACTTCG AGGCGGTGGC CAACTGCATC 
AATGCCAAGC TGAAGCGCCG CAATGCCAAC AGCGACTATA GTTTCAAAAC CAAGGACCAA 
GTTCGCCAGC ACTATTACCA GACCCATCAC AAGATTTGCA AATATGTGCG CTTCTCGGAG 
GAACTGAAAA AGCCCGCCCA GGAGTTGTAC ACGCTGATCA ACTACGGCGA GATGCGGCGC 
AAGCTGCAGT TCCTCACGGA GAAGCACTTC ATGAAGCTGA AGCAGCTGGT CTACCAGGGC 
CAAATAACCG TGCGTTGCAA GGGCAAGAAC ATTCGCATTA AGACGCCATC GTGCAAGGCG 
CTCCGGCGGC TAAATCAATT GGATGACTCA CTGGAGGATA TCCGGCTGCC CAGCAAGGTG 
GAGGTGCTGG TGACTCCCGC CAATATGGAG GCCTTCGGAC GCGTCCAGTC ACTGGCGCAG 
AATCCACGCG GCAGGATCAT AGTTCCGCTG CACAAGAAAC TAATCAGCTT CATCAAGACG 
TTCGAGTACA AATGGCGCAG CGCCAATCAG CGTTTGCACG AGGAGAAGAG TGCATACTTT 
TCCAGCAGCC TGCCATCTGC TGCGTCCAAC AACAATAACA ACAACAATGA AACGGAACCA 
TTACAGCCGT CAGTTGCATC GCTGGATCCC TCAATGTGCT TCCAGCCCCG GCCGGGAGTG 
GCCATCCACC GACCTTTGCT CAGCATCACC GCCTACCTAA GCAGCATCAG TATTTGCTTG 
ACGGCATACG AGGAGCGCAT GGGCTTCAAA GTGCGCAGCG AGACCTTGGG CAATTTGGCC 
GGCATGTCGG TGGCGGCCAA CAAGCGACTA CGAACGGAAA GCGGCTCCGA GAAGCGTTCG 
CCGGAGACAA AGAAACCCAA ATCGAGTGCC AGCCCGCCGC TGGAGAAGAG TTTGGACGAC 
GGGCCCTTGG AGGGCAATCT GATGAAGATG GAGAATAGCA GCGGTGATGA GCTGGGCGAG 
GAGATTCATG AGTTTCTGGG CGATATATTG GAGGCCATGC AGCATCCGCA AGCAGCCACT 
ATTCCAGCTC TTTCGGCCAC CACTGGTGAT ACCACAACTG TTGCTGTTGC GCTTGAAACA 
TCGCACGATC CTGTACAACA GGCTTATCCG GCCAACGCGG ATCTATCTAA TGCCATGGCG 
ACATCTGTTC TACAAACTTC CTGTGCTGCA GCTCCCGCTC CTTCGACTCC TGTAACCGGT 
TCTTTGGCGG CTCCATCCGT AGCCCGCTCC AAACGCAAAG AAGCTAAGGA GGCGGCGGCC 
GCGGCACAGG CTCGAAATTT TAAGCCACTG CTCAGCGACG ATATACTGAA GAGAATCCGC 
AAGGGCTGGA CGCAGGCGAA TGCGGCGGAT ATAACCATCG GTGATCTGTA TGTCGTTTTT 
GGCCAGGATT CCAAGCTGGA GCTGGAGTAT TATTGGTGTG AGGTCGACAG TTCGACGGCC 
ATGGCATCCA GCATACTAAC AATCAACACG GTAACGCCTA GCTCCTCGTC GGTGGCCACC 
CAAACCGGCA GCGCCGCCTC GAACGCCAAC CAAACAGGCG CATCTTCCAA CTGCTACGTC 
AGCGCATCGA GCAATTCGAG CACCTCGAGC ACTTCGCTGC CCTACAATCC CAACGACTGT 
GATAGCGTGG AACGCGTCAG GGCCGTCACC ACATCGTCGG TGAGCAACAA GCTGAAGCAC 
CTGCTACTCG TGGCCAATCT CAGCGAGCGC GTGCGCAAGC GCCAATGCAA CTGCGGCCAC 
ACGTGCGATC GCAAGCGGGA CCTGATGACC AAGGCGCAGC AGCTGGCAGA GGCGACGGCC 
ACCGGCGGAG TGGGCGGCAT GGTTGAGGGC AATTTCCGAA CGCCGATGCT GCCAGTGCGG 
CGGCCGATAT CCAACATCGA TCCTGTGCGC CAACTCTCAG CGCTCACACG GCAAAAGATC 
AGTCGTCAGG TGCTTGTGCA GCGCCGTCTC CTACCACCCA CATCCGCTGG CGATCGTCCG 
TACGATCTGC TGAGTGTGCG CCAACTCCAC AGTGGCCTTT TCGAGCCGAT TGATCGCGTG 
GATGGCATAA GTTCCGCTGG CATCAGCACC TCTGGCTCGA AACCCGATTC CAGCATGAAT 
GCCACGACCG CTTCACAGGA TCAAGAGCCA GGGGATCAGG GGGCATTGGA GTTCCTCAAC 
GACGAGGCCA CGCAGGTCAG CGCTCGAGAC ATGCCCAATC TGGACATTTG CGTGGCCACC 
AACAGGACGG ACGTCAGCAG TTCCCTGAAC GAGGCGGCCC AAGACGGGAG CACAACTCAA 
AGCTTCTTCC AAGGCAGCAT GAGTCCGATG CACTTGCTCA GAGATTCAAC ATCCAACGCA 
CGCTGGCTGG AGGACAATAT CAATGACTTC TCGTTGACGT CGCTGCTGGG GCATTTGGAT 
GAGATTGATG CCACCAGGGA TATACTGGAT CCGTCGTCAA GCATGTCGGT GATCAGCGAG 
AGCAGCGTGG ACTTTCGGCA CAAGTTCCAG GAAATCGCCG CCCTGCTGCA GCAGCAGGAG 
AAAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crm ( NM_057757.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477105.2 Drosophila melanogaster cramped (crm), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00650
Clone ID Related Accession (Same CDS sequence)	NM_166951.2 , NM_057757.4
Accession Version	NM_057757.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cramped, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057757.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAC TGTCCAAGCA GCCGCCGCCA CCCCCGCTGA CGCAGCCGCC GCCGCCGTCG 
TCATCCGTGT CCATTGAGGA GCCGCTGCCA AATGGCAAGG GAGGAGGAGC AGTAGTTGTT 
AACTCGATAG CTAAGCTGCC CGAGGAGGAG CTCCTCGGCT CGGTGACCAT GCACAATTGT 
CCGGGAACGC GGGCCAGTGC CCGGGTCATA CAGAAGATGA AGCAGGACCA GACGCGCCCG 
ATGACACCGC CGCCCAGCGA ACGGGAGCCG AACAAAAAGG AGGAGAAGGC CGCCCAAAAG 
ACGCCCAGCC AGCTGAAGAC GGGCAGCGGG AAGACCACCT GGACGAATGT GGAGCGCAAC 
TGCTTCTTCG ACGCCCTCAA CGAATTCGGC AAGGACTTCG AGGCGGTGGC CAACTGCATC 
AATGCCAAGC TGAAGCGCCG CAATGCCAAC AGCGACTATA GTTTCAAAAC CAAGGACCAA 
GTTCGCCAGC ACTATTACCA GACCCATCAC AAGATTTGCA AATATGTGCG CTTCTCGGAG 
GAACTGAAAA AGCCCGCCCA GGAGTTGTAC ACGCTGATCA ACTACGGCGA GATGCGGCGC 
AAGCTGCAGT TCCTCACGGA GAAGCACTTC ATGAAGCTGA AGCAGCTGGT CTACCAGGGC 
CAAATAACCG TGCGTTGCAA GGGCAAGAAC ATTCGCATTA AGACGCCATC GTGCAAGGCG 
CTCCGGCGGC TAAATCAATT GGATGACTCA CTGGAGGATA TCCGGCTGCC CAGCAAGGTG 
GAGGTGCTGG TGACTCCCGC CAATATGGAG GCCTTCGGAC GCGTCCAGTC ACTGGCGCAG 
AATCCACGCG GCAGGATCAT AGTTCCGCTG CACAAGAAAC TAATCAGCTT CATCAAGACG 
TTCGAGTACA AATGGCGCAG CGCCAATCAG CGTTTGCACG AGGAGAAGAG TGCATACTTT 
TCCAGCAGCC TGCCATCTGC TGCGTCCAAC AACAATAACA ACAACAATGA AACGGAACCA 
TTACAGCCGT CAGTTGCATC GCTGGATCCC TCAATGTGCT TCCAGCCCCG GCCGGGAGTG 
GCCATCCACC GACCTTTGCT CAGCATCACC GCCTACCTAA GCAGCATCAG TATTTGCTTG 
ACGGCATACG AGGAGCGCAT GGGCTTCAAA GTGCGCAGCG AGACCTTGGG CAATTTGGCC 
GGCATGTCGG TGGCGGCCAA CAAGCGACTA CGAACGGAAA GCGGCTCCGA GAAGCGTTCG 
CCGGAGACAA AGAAACCCAA ATCGAGTGCC AGCCCGCCGC TGGAGAAGAG TTTGGACGAC 
GGGCCCTTGG AGGGCAATCT GATGAAGATG GAGAATAGCA GCGGTGATGA GCTGGGCGAG 
GAGATTCATG AGTTTCTGGG CGATATATTG GAGGCCATGC AGCATCCGCA AGCAGCCACT 
ATTCCAGCTC TTTCGGCCAC CACTGGTGAT ACCACAACTG TTGCTGTTGC GCTTGAAACA 
TCGCACGATC CTGTACAACA GGCTTATCCG GCCAACGCGG ATCTATCTAA TGCCATGGCG 
ACATCTGTTC TACAAACTTC CTGTGCTGCA GCTCCCGCTC CTTCGACTCC TGTAACCGGT 
TCTTTGGCGG CTCCATCCGT AGCCCGCTCC AAACGCAAAG AAGCTAAGGA GGCGGCGGCC 
GCGGCACAGG CTCGAAATTT TAAGCCACTG CTCAGCGACG ATATACTGAA GAGAATCCGC 
AAGGGCTGGA CGCAGGCGAA TGCGGCGGAT ATAACCATCG GTGATCTGTA TGTCGTTTTT 
GGCCAGGATT CCAAGCTGGA GCTGGAGTAT TATTGGTGTG AGGTCGACAG TTCGACGGCC 
ATGGCATCCA GCATACTAAC AATCAACACG GTAACGCCTA GCTCCTCGTC GGTGGCCACC 
CAAACCGGCA GCGCCGCCTC GAACGCCAAC CAAACAGGCG CATCTTCCAA CTGCTACGTC 
AGCGCATCGA GCAATTCGAG CACCTCGAGC ACTTCGCTGC CCTACAATCC CAACGACTGT 
GATAGCGTGG AACGCGTCAG GGCCGTCACC ACATCGTCGG TGAGCAACAA GCTGAAGCAC 
CTGCTACTCG TGGCCAATCT CAGCGAGCGC GTGCGCAAGC GCCAATGCAA CTGCGGCCAC 
ACGTGCGATC GCAAGCGGGA CCTGATGACC AAGGCGCAGC AGCTGGCAGA GGCGACGGCC 
ACCGGCGGAG TGGGCGGCAT GGTTGAGGGC AATTTCCGAA CGCCGATGCT GCCAGTGCGG 
CGGCCGATAT CCAACATCGA TCCTGTGCGC CAACTCTCAG CGCTCACACG GCAAAAGATC 
AGTCGTCAGG TGCTTGTGCA GCGCCGTCTC CTACCACCCA CATCCGCTGG CGATCGTCCG 
TACGATCTGC TGAGTGTGCG CCAACTCCAC AGTGGCCTTT TCGAGCCGAT TGATCGCGTG 
GATGGCATAA GTTCCGCTGG CATCAGCACC TCTGGCTCGA AACCCGATTC CAGCATGAAT 
GCCACGACCG CTTCACAGGA TCAAGAGCCA GGGGATCAGG GGGCATTGGA GTTCCTCAAC 
GACGAGGCCA CGCAGGTCAG CGCTCGAGAC ATGCCCAATC TGGACATTTG CGTGGCCACC 
AACAGGACGG ACGTCAGCAG TTCCCTGAAC GAGGCGGCCC AAGACGGGAG CACAACTCAA 
AGCTTCTTCC AAGGCAGCAT GAGTCCGATG CACTTGCTCA GAGATTCAAC ATCCAACGCA 
CGCTGGCTGG AGGACAATAT CAATGACTTC TCGTTGACGT CGCTGCTGGG GCATTTGGAT 
GAGATTGATG CCACCAGGGA TATACTGGAT CCGTCGTCAA GCATGTCGGT GATCAGCGAG 
AGCAGCGTGG ACTTTCGGCA CAAGTTCCAG GAAATCGCCG CCCTGCTGCA GCAGCAGGAG 
AAAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477105.2
CDS	77..3025
Translation	MEELSKQPPPPPLTQPPPPSSSVSIEEPLPNGKGGGAVVVNSIAKLPEEELLGSVTMHNCPGTRASARVIQKMKQDQTRPMTPPPSEREPNKKEEKAAQKTPSQLKTGSGKTTWTNVERNCFFDALNEFGKDFEAVANCINAKLKRRNANSDYSFKTKDQVRQHYYQTHHKICKYVRFSEELKKPAQELYTLINYGEMRRKLQFLTEKHFMKLKQLVYQGQITVRCKGKNIRIKTPSCKALRRLNQLDDSLEDIRLPSKVEVLVTPANMEAFGRVQSLAQNPRGRIIVPLHKKLISFIKTFEYKWRSANQRLHEEKSAYFSSSLPSAASNNNNNNNETEPLQPSVASLDPSMCFQPRPGVAIHRPLLSITAYLSSISICLTAYEERMGFKVRSETLGNLAGMSVAANKRLRTESGSEKRSPETKKPKSSASPPLEKSLDDGPLEGNLMKMENSSGDELGEEIHEFLGDILEAMQHPQAATIPALSATTGDTTTVAVALETSHDPVQQAYPANADLSNAMATSVLQTSCAAAPAPSTPVTGSLAAPSVARSKRKEAKEAAAAAQARNFKPLLSDDILKRIRKGWTQANAADITIGDLYVVFGQDSKLELEYYWCEVDSSTAMASSILTINTVTPSSSSVATQTGSAASNANQTGASSNCYVSASSNSSTSSTSLPYNPNDCDSVERVRAVTTSSVSNKLKHLLLVANLSERVRKRQCNCGHTCDRKRDLMTKAQQLAEATATGGVGGMVEGNFRTPMLPVRRPISNIDPVRQLSALTRQKISRQVLVQRRLLPPTSAGDRPYDLLSVRQLHSGLFEPIDRVDGISSAGISTSGSKPDSSMNATTASQDQEPGDQGALEFLNDEATQVSARDMPNLDICVATNRTDVSSSLNEAAQDGSTTQSFFQGSMSPMHLLRDSTSNARWLEDNINDFSLTSLLGHLDEIDATRDILDPSSSMSVISESSVDFRHKFQEIAALLQQQEKD

Target ORF information:

RefSeq Version	NM_057757.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cramped (crm), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057757.4

ORF Insert Sequence:

ATGGAGGAAC TGTCCAAGCA GCCGCCGCCA CCCCCGCTGA CGCAGCCGCC GCCGCCGTCG 
TCATCCGTGT CCATTGAGGA GCCGCTGCCA AATGGCAAGG GAGGAGGAGC AGTAGTTGTT 
AACTCGATAG CTAAGCTGCC CGAGGAGGAG CTCCTCGGCT CGGTGACCAT GCACAATTGT 
CCGGGAACGC GGGCCAGTGC CCGGGTCATA CAGAAGATGA AGCAGGACCA GACGCGCCCG 
ATGACACCGC CGCCCAGCGA ACGGGAGCCG AACAAAAAGG AGGAGAAGGC CGCCCAAAAG 
ACGCCCAGCC AGCTGAAGAC GGGCAGCGGG AAGACCACCT GGACGAATGT GGAGCGCAAC 
TGCTTCTTCG ACGCCCTCAA CGAATTCGGC AAGGACTTCG AGGCGGTGGC CAACTGCATC 
AATGCCAAGC TGAAGCGCCG CAATGCCAAC AGCGACTATA GTTTCAAAAC CAAGGACCAA 
GTTCGCCAGC ACTATTACCA GACCCATCAC AAGATTTGCA AATATGTGCG CTTCTCGGAG 
GAACTGAAAA AGCCCGCCCA GGAGTTGTAC ACGCTGATCA ACTACGGCGA GATGCGGCGC 
AAGCTGCAGT TCCTCACGGA GAAGCACTTC ATGAAGCTGA AGCAGCTGGT CTACCAGGGC 
CAAATAACCG TGCGTTGCAA GGGCAAGAAC ATTCGCATTA AGACGCCATC GTGCAAGGCG 
CTCCGGCGGC TAAATCAATT GGATGACTCA CTGGAGGATA TCCGGCTGCC CAGCAAGGTG 
GAGGTGCTGG TGACTCCCGC CAATATGGAG GCCTTCGGAC GCGTCCAGTC ACTGGCGCAG 
AATCCACGCG GCAGGATCAT AGTTCCGCTG CACAAGAAAC TAATCAGCTT CATCAAGACG 
TTCGAGTACA AATGGCGCAG CGCCAATCAG CGTTTGCACG AGGAGAAGAG TGCATACTTT 
TCCAGCAGCC TGCCATCTGC TGCGTCCAAC AACAATAACA ACAACAATGA AACGGAACCA 
TTACAGCCGT CAGTTGCATC GCTGGATCCC TCAATGTGCT TCCAGCCCCG GCCGGGAGTG 
GCCATCCACC GACCTTTGCT CAGCATCACC GCCTACCTAA GCAGCATCAG TATTTGCTTG 
ACGGCATACG AGGAGCGCAT GGGCTTCAAA GTGCGCAGCG AGACCTTGGG CAATTTGGCC 
GGCATGTCGG TGGCGGCCAA CAAGCGACTA CGAACGGAAA GCGGCTCCGA GAAGCGTTCG 
CCGGAGACAA AGAAACCCAA ATCGAGTGCC AGCCCGCCGC TGGAGAAGAG TTTGGACGAC 
GGGCCCTTGG AGGGCAATCT GATGAAGATG GAGAATAGCA GCGGTGATGA GCTGGGCGAG 
GAGATTCATG AGTTTCTGGG CGATATATTG GAGGCCATGC AGCATCCGCA AGCAGCCACT 
ATTCCAGCTC TTTCGGCCAC CACTGGTGAT ACCACAACTG TTGCTGTTGC GCTTGAAACA 
TCGCACGATC CTGTACAACA GGCTTATCCG GCCAACGCGG ATCTATCTAA TGCCATGGCG 
ACATCTGTTC TACAAACTTC CTGTGCTGCA GCTCCCGCTC CTTCGACTCC TGTAACCGGT 
TCTTTGGCGG CTCCATCCGT AGCCCGCTCC AAACGCAAAG AAGCTAAGGA GGCGGCGGCC 
GCGGCACAGG CTCGAAATTT TAAGCCACTG CTCAGCGACG ATATACTGAA GAGAATCCGC 
AAGGGCTGGA CGCAGGCGAA TGCGGCGGAT ATAACCATCG GTGATCTGTA TGTCGTTTTT 
GGCCAGGATT CCAAGCTGGA GCTGGAGTAT TATTGGTGTG AGGTCGACAG TTCGACGGCC 
ATGGCATCCA GCATACTAAC AATCAACACG GTAACGCCTA GCTCCTCGTC GGTGGCCACC 
CAAACCGGCA GCGCCGCCTC GAACGCCAAC CAAACAGGCG CATCTTCCAA CTGCTACGTC 
AGCGCATCGA GCAATTCGAG CACCTCGAGC ACTTCGCTGC CCTACAATCC CAACGACTGT 
GATAGCGTGG AACGCGTCAG GGCCGTCACC ACATCGTCGG TGAGCAACAA GCTGAAGCAC 
CTGCTACTCG TGGCCAATCT CAGCGAGCGC GTGCGCAAGC GCCAATGCAA CTGCGGCCAC 
ACGTGCGATC GCAAGCGGGA CCTGATGACC AAGGCGCAGC AGCTGGCAGA GGCGACGGCC 
ACCGGCGGAG TGGGCGGCAT GGTTGAGGGC AATTTCCGAA CGCCGATGCT GCCAGTGCGG 
CGGCCGATAT CCAACATCGA TCCTGTGCGC CAACTCTCAG CGCTCACACG GCAAAAGATC 
AGTCGTCAGG TGCTTGTGCA GCGCCGTCTC CTACCACCCA CATCCGCTGG CGATCGTCCG 
TACGATCTGC TGAGTGTGCG CCAACTCCAC AGTGGCCTTT TCGAGCCGAT TGATCGCGTG 
GATGGCATAA GTTCCGCTGG CATCAGCACC TCTGGCTCGA AACCCGATTC CAGCATGAAT 
GCCACGACCG CTTCACAGGA TCAAGAGCCA GGGGATCAGG GGGCATTGGA GTTCCTCAAC 
GACGAGGCCA CGCAGGTCAG CGCTCGAGAC ATGCCCAATC TGGACATTTG CGTGGCCACC 
AACAGGACGG ACGTCAGCAG TTCCCTGAAC GAGGCGGCCC AAGACGGGAG CACAACTCAA 
AGCTTCTTCC AAGGCAGCAT GAGTCCGATG CACTTGCTCA GAGATTCAAC ATCCAACGCA 
CGCTGGCTGG AGGACAATAT CAATGACTTC TCGTTGACGT CGCTGCTGGG GCATTTGGAT 
GAGATTGATG CCACCAGGGA TATACTGGAT CCGTCGTCAA GCATGTCGGT GATCAGCGAG 
AGCAGCGTGG ACTTTCGGCA CAAGTTCCAG GAAATCGCCG CCCTGCTGCA GCAGCAGGAG 
AAAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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crm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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