boi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	boi
Entrez Gene ID	31229
Full Name	brother of ihog
Synonyms	Boi,CG13756,CG32796,CG7894,CT23737,CT33235,Dmel\CG32796,EG:BACH59J11.2
General protein information	Preferred Names brother of ihog Names CG32796 gene product from transcript CG32796-RB CG32796-PA CG32796-PB CG32796-PE CG32796-PF CG32796-PG boi-PA boi-PB boi-PE boi-PF boi-PG brother of iHog
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3A3-3A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001201611.2	NP_001188540.1	brother of ihog, isoform E
NM_166940.3	NP_726811.1	brother of ihog, isoform B
NM_166941.3	NP_726812.1	brother of ihog, isoform A
NM_001201612.2	NP_001188541.1	brother of ihog, isoform F
NM_001103398.4	NP_001096868.3	brother of ihog, isoform G

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	ihog	NP_609085.1
Drosophila melanogaster (fruit fly)	boi	NP_726811.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002040	XP_003436011.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ihog and Boi elicit Hh signaling via Ptc but do not aid Ptc in sequestering the Hh ligand
Title: Ihog and Boi elicit Hh signaling via Ptc but do not aid Ptc in sequestering the Hh ligand.

miR-932 can modulate Hh activity by specifically targeting Boi in Drosophila.
Title: Drosophila miR-932 modulates hedgehog signaling by targeting its co-receptor Brother of ihog.

analysis of balancing between hedgehog, a retention and release equilibrium given by Dally, Ihog, Boi and shifted/DmWif
Title: Balancing Hedgehog, a retention and release equilibrium given by Dally, Ihog, Boi and shifted/DmWif.

Shifted (Shf) regulates short-range hedgehog (Hh) signaling through interactions with the receptor complex interference Hh (Ihog) and brother of Ihog (Boi).
Title: The Drosophila WIF1 homolog Shifted maintains glypican-independent Hedgehog signaling and interacts with the Hedgehog co-receptors Ihog and Boi.

Drosophila Boi limits Hedgehog levels to suppress follicle stem cell proliferation
Title: Drosophila Boi limits Hedgehog levels to suppress follicle stem cell proliferation.

The following boi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the boi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00607	NM_166941.3 Latest version!	Drosophila melanogaster brother of ihog (boi), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa00605	NM_001201612.2 Latest version!	Drosophila melanogaster brother of ihog (boi), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00605	NM_001201611.2 Latest version!	Drosophila melanogaster brother of ihog (boi), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00605	NM_166940.3 Latest version!	Drosophila melanogaster brother of ihog (boi), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00606	NM_001103398.4 Latest version!	Drosophila melanogaster brother of ihog (boi), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

boi ( NM_166941.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726812.1 Drosophila melanogaster brother of ihog (boi), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00607
Clone ID Related Accession (Same CDS sequence)	NM_166941.3
Accession Version	NM_166941.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brother of ihog, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_166941.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCTGGT CTGGCAACGC CAGATATTCA AATGTTCAGA ATTCGAGCAT GGTGCCTCCG 
TCACGACCCA ATGTCACCCG TCTGTCGGAC GAGTCCGTGA TGCTGCGCTG GAGTGTGCCA 
CAAAACTCTG GACTCCAGAT CACCTTTTTC AAGGTGCAGT ATCGCATGTT GGGCGACGGA 
AAAAATCGGA GGAAGAACTG GCAGACGACC AGCGACAATA TCCCGTACGG AAACGCGCAC 
GGATCCGGGC CTGGCCAGGG GAACAGTCAC TGGCGGGCGC GGAACCGGGA CAGGGAGTAT 
GAAATGGGCA ATCCGCCCAA GAACTTCACC TCCTCCGTTA CGGGACTGAC CGCCGGCAAG 
TACTACCGCT TCCGGATCGT GGCGGTGTAC TCCAATAACG ACAACAAGGA GGGCAACACC 
TCCCTCAAGT TCTTCCTGCA GAATGGAACG TCCAAGTCGA ATCTGCCAGT GCCGGAACTA 
CGCGAGGTGG AGACGCTTTC CGAGTCGGCC GTGGTGCTCC ATTGGTCCCT GGTATCCTCG 
ACTCAAAATG AGGATATAGA CGGGTACTAT GCCTACTATA GGCCAGCGGA CTCAGCAGCG 
GAGTATTTGA AGGCCACGGT GGATGGCGGA ATGAGCAGGA GCTTCAAGAT AGACCTCCTT 
CGTCCAGGAA CCCCTTATGA GTTCAAATTG CAATCGTTCA ACTCCGACGC AGCCTCTGAA 
TTCAGTGCTA TTCGGCAAGC GAGGACCAGA AAAACGCACG AGCAACCGGC TTCATCTTCG 
GCCACGCCAG TGCCAGCCAA TAAGGCAGTG GAACAGCACC AGAACTCCTT ATATCCACTG 
ATCGCAGGTG CCGCTGGCGG AGGCATACTA CTCCTCATCG CCTCATTGGT GGTCTGCCTT 
TGCCTAAAGA GACGGGAAAA CTCTCAGCCG GAGGATGAGA ACAAACCGCA ATTGGAACAC 
ATTCAAGCGG ACTTTGTTAC CTCCGCCGTC CTGGGAGTTG GTGGCCACCA CAAAAGTGGC 
GATGTAAGGC GCCTCAACGG AGTGATTCCG AGGATGAATA TTACGCCCAA TCCGCTGGCC 
CAGGAAACGG CATCCGATAA GAACAACTCT GGACTGCATC AGAATGGCCT TCACCACGCA 
CAGCCGTACC ATCCGCCTGG CACGCCCACT CTGATGCACA AGCGACTGGA CTACCACCAG 
CAGCCGCCGC CGGTGCCTCC GCACTCGGCC TATTACCAAC AGGCACCCAC GCACCAGCCG 
TCGCCGATGA TGGAGCGCAG TGTCAGGGGT CACCAGCAGC CCGGGGGCTT TCATATGGAG 
GAGGGCACAC CCACGCCGAC CAGGATACCA TCCCTGCGGC GACAGCGACG CACCTCGGGC 
AACCAGCAGG TGCACAACAG CCACAGCAAT CTCAATATTG GCCTGAGCCA CCACAACAAC 
AACAACAACA ACCTGCCGCC GCACCACCAG CATCTGCACC ACCACCCCGG CCATCCGGGT 
GGCCTGGTGG GCATACCCAT CGTGCCAGGG AGCCCTAGGG TCCAGAGATC GCCGATGCCG 
AGCAGGGCGA TGATCAAGAG GACGCGCCTG GGCAGCCACA CGGACAACAT ATCGTCGGGC 
AGTCTGAACA GTATTGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726812.1
CDS	194..1837
Translation	MIWSGNARYSNVQNSSMVPPSRPNVTRLSDESVMLRWSVPQNSGLQITFFKVQYRMLGDGKNRRKNWQTTSDNIPYGNAHGSGPGQGNSHWRARNRDREYEMGNPPKNFTSSVTGLTAGKYYRFRIVAVYSNNDNKEGNTSLKFFLQNGTSKSNLPVPELREVETLSESAVVLHWSLVSSTQNEDIDGYYAYYRPADSAAEYLKATVDGGMSRSFKIDLLRPGTPYEFKLQSFNSDAASEFSAIRQARTRKTHEQPASSSATPVPANKAVEQHQNSLYPLIAGAAGGGILLLIASLVVCLCLKRRENSQPEDENKPQLEHIQADFVTSAVLGVGGHHKSGDVRRLNGVIPRMNITPNPLAQETASDKNNSGLHQNGLHHAQPYHPPGTPTLMHKRLDYHQQPPPVPPHSAYYQQAPTHQPSPMMERSVRGHQQPGGFHMEEGTPTPTRIPSLRRQRRTSGNQQVHNSHSNLNIGLSHHNNNNNNLPPHHQHLHHHPGHPGGLVGIPIVPGSPRVQRSPMPSRAMIKRTRLGSHTDNISSGSLNSIEV

Target ORF information:

RefSeq Version	NM_166941.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brother of ihog (boi), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166941.3

ORF Insert Sequence:

ATGATCTGGT CTGGCAACGC CAGATATTCA AATGTTCAGA ATTCGAGCAT GGTGCCTCCG 
TCACGACCCA ATGTCACCCG TCTGTCGGAC GAGTCCGTGA TGCTGCGCTG GAGTGTGCCA 
CAAAACTCTG GACTCCAGAT CACCTTTTTC AAGGTGCAGT ATCGCATGTT GGGCGACGGA 
AAAAATCGGA GGAAGAACTG GCAGACGACC AGCGACAATA TCCCGTACGG AAACGCGCAC 
GGATCCGGGC CTGGCCAGGG GAACAGTCAC TGGCGGGCGC GGAACCGGGA CAGGGAGTAT 
GAAATGGGCA ATCCGCCCAA GAACTTCACC TCCTCCGTTA CGGGACTGAC CGCCGGCAAG 
TACTACCGCT TCCGGATCGT GGCGGTGTAC TCCAATAACG ACAACAAGGA GGGCAACACC 
TCCCTCAAGT TCTTCCTGCA GAATGGAACG TCCAAGTCGA ATCTGCCAGT GCCGGAACTA 
CGCGAGGTGG AGACGCTTTC CGAGTCGGCC GTGGTGCTCC ATTGGTCCCT GGTATCCTCG 
ACTCAAAATG AGGATATAGA CGGGTACTAT GCCTACTATA GGCCAGCGGA CTCAGCAGCG 
GAGTATTTGA AGGCCACGGT GGATGGCGGA ATGAGCAGGA GCTTCAAGAT AGACCTCCTT 
CGTCCAGGAA CCCCTTATGA GTTCAAATTG CAATCGTTCA ACTCCGACGC AGCCTCTGAA 
TTCAGTGCTA TTCGGCAAGC GAGGACCAGA AAAACGCACG AGCAACCGGC TTCATCTTCG 
GCCACGCCAG TGCCAGCCAA TAAGGCAGTG GAACAGCACC AGAACTCCTT ATATCCACTG 
ATCGCAGGTG CCGCTGGCGG AGGCATACTA CTCCTCATCG CCTCATTGGT GGTCTGCCTT 
TGCCTAAAGA GACGGGAAAA CTCTCAGCCG GAGGATGAGA ACAAACCGCA ATTGGAACAC 
ATTCAAGCGG ACTTTGTTAC CTCCGCCGTC CTGGGAGTTG GTGGCCACCA CAAAAGTGGC 
GATGTAAGGC GCCTCAACGG AGTGATTCCG AGGATGAATA TTACGCCCAA TCCGCTGGCC 
CAGGAAACGG CATCCGATAA GAACAACTCT GGACTGCATC AGAATGGCCT TCACCACGCA 
CAGCCGTACC ATCCGCCTGG CACGCCCACT CTGATGCACA AGCGACTGGA CTACCACCAG 
CAGCCGCCGC CGGTGCCTCC GCACTCGGCC TATTACCAAC AGGCACCCAC GCACCAGCCG 
TCGCCGATGA TGGAGCGCAG TGTCAGGGGT CACCAGCAGC CCGGGGGCTT TCATATGGAG 
GAGGGCACAC CCACGCCGAC CAGGATACCA TCCCTGCGGC GACAGCGACG CACCTCGGGC 
AACCAGCAGG TGCACAACAG CCACAGCAAT CTCAATATTG GCCTGAGCCA CCACAACAAC 
AACAACAACA ACCTGCCGCC GCACCACCAG CATCTGCACC ACCACCCCGG CCATCCGGGT 
GGCCTGGTGG GCATACCCAT CGTGCCAGGG AGCCCTAGGG TCCAGAGATC GCCGATGCCG 
AGCAGGGCGA TGATCAAGAG GACGCGCCTG GGCAGCCACA CGGACAACAT ATCGTCGGGC 
AGTCTGAACA GTATTGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

boi ( NM_001201611.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188540.1 Drosophila melanogaster brother of ihog (boi), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa00605
Clone ID Related Accession (Same CDS sequence)	NM_001201611.2 , NM_166940.3 , NM_001201612.2
Accession Version	NM_001201611.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brother of ihog, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001201611.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGAAA TCAGGCTAGT TGTGGTTCTA CTGGTCCTTA TGACCACCTG TATCTATACA 
CTTCAAGCCA GTGACAGTAA TTCCTCCGTG GCAACCACCT TGGGCGTTTT GTTCGAGAGG 
GCTCCCGAAT CAGCGGTGGC CCCGAAAGGA GATGAGGTGG TCTTCGAGTG CGAGCTGAAT 
CTGAAACCTG ATCGCCTGGA GTGGCGGTTT AGACGTGGTG ATTCCTCGGA GCCCTATCTA 
TACCTAAGGC CCTCGGCTGG CTATAACGTG ACCAGCGGAA GCGATGGTCT TGCCTGGAGA 
CTGCGCATCT ATGTGAGTGC CCAAACGGCT GGGGACTACC AGTGTGTGGC TTGGTATGGC 
CCGGGTGCAC TGGCTTCCAC GCCAGCCCGC TTGGCCTTGG TCTCGATTGC TTTGGACGTC 
GGCCAGGGAT CCATGGGGGC CAGGAGCTCC ATCCGCTGGA GCGTGGCGCC CAAAAACTGC 
CTGCTCATTC GTTGCGGCAG CGTAATATCG AATCCGCCGG CTATTTGGAG CTTCTACAGA 
AACGGAAAGA AGCTACCTCA ATCGGAGCTG CTGCCTGGCG CTGCGGGGGC ATTGGTTTTG 
GATACGGTCA CCGCCAAAGA TGCGGGTAAC TACTCGTGCG TGGCCACGAA CTCCATAACA 
GGCGATGAAC TTAGGCTCCC CCAAACTATT GAACTAAGAG TGGACTACAC AGATAGGACA 
CCTCCGTACT TTCTACAACG TCCACCCATC GAATACTCAG CTCGTCCCGG AGAAACAGTG 
GTGCTCGAGT GTCCTGGCGT GGGATCCCCA CGACCCCGAG TCATTTGGAG TAGTCCCAAT 
GTGGTTGAGA TCTATAATAA TAGAAGCACC ATCCTGTCCT ACGGCCTGCA AATCACCGAT 
GTGAAGCCCG AGGATCAGGG TTCCTATATC TGCATGCTGG ACAATGGTAT TGCACCACCG 
ATCGATCACA CCATCAAGTT GAGTGTTTTG CAGCGGCCCA CGATCTTAAG AGGACCGGCC 
GCCACTCTGA CGAATGAGAG CAATCCTTTG CTGCTGGATT GCTCCGCCTC GGGGAATCCC 
CAACCCGATA TTTATTGGCT CATAAACGGT GAGGATGCCA CCAAAGATCC GGAGGCTGTG 
GTGGATAACC GGAGCCTGCA GCTGAAGCGA GTTCAAAAGC GTCACGCTGG CGTGGTTCAG 
TGCTTTGCAA AGAACATTTT GGGAGAGACG AGCGAGGGAA ACATATTGCG CGTGAATCCC 
CTGGAAATCA CCGGCGAGGA TGACGAACCA CTTGGCGAAG TTCCCGTGTG GCCAGTTCAT 
GAATCCAACA CTGGAATGGG CACCTCGTCA ACATCTGGAG GCTCCAAAAA CAAGAGCGGC 
AGACGAAAGT TTAAGGCGAG CATGGTGCCT CCGTCACGAC CCAATGTCAC CCGTCTGTCG 
GACGAGTCCG TGATGCTGCG CTGGAGTGTG CCACAAAACT CTGGACTCCA GATCACCTTT 
TTCAAGGTGC AGTATCGCAT GTTGGGCGAC GGAAAAAATC GGAGGAAGAA CTGGCAGACG 
ACCAGCGACA ATATCCCGTA CGGAAACGCG CACGGATCCG GGCCTGGCCA GGGGAACAGT 
CACTGGCGGG CGCGGAACCG GGACAGGGAG TATGAAATGG GCAATCCGCC CAAGAACTTC 
ACCTCCTCCG TTACGGGACT GACCGCCGGC AAGTACTACC GCTTCCGGAT CGTGGCGGTG 
TACTCCAATA ACGACAACAA GGAGGGCAAC ACCTCCCTCA AGTTCTTCCT GCAGAATGGA 
ACGTCCAAGT CGAATCTGCC AGTGCCGGAA CTACGCGAGG TGGAGACGCT TTCCGAGTCG 
GCCGTGGTGC TCCATTGGTC CCTGGTATCC TCGACTCAAA ATGAGGATAT AGACGGGTAC 
TATGCCTACT ATAGGCCAGC GGACTCAGCA GCGGAGTATT TGAAGGCCAC GGTGGATGGC 
GGAATGAGCA GGAGCTTCAA GATAGACCTC CTTCGTCCAG GAACCCCTTA TGAGTTCAAA 
TTGCAATCGT TCAACTCCGA CGCAGCCTCT GAATTCAGTG CTATTCGGCA AGCGAGGACC 
AGAAAAACGC ACGAGCAACC GGCTTCATCT TCGGCCACGC CAGTGCCAGC CAATAAGGCA 
GTGGAACAGC ACCAGAACTC CTTATATCCA CTGATCGCAG GTGCCGCTGG CGGAGGCATA 
CTACTCCTCA TCGCCTCATT GGTGGTCTGC CTTTGCCTAA AGAGACGGGA AAACTCTCAG 
CCGGAGGATG AGAACAAACC GCAATTGGAA CACATTCAAG CGGACTTTGT TACCTCCGCC 
GTCCTGGGAG TTGGTGGCCA CCACAAAAGT GGCGATGTAA GGCGCCTCAA CGGAGTGATT 
CCGAGGATGA ATATTACGCC CAATCCGCTG GCCCAGGAAA CGGCATCCGA TAAGAACAAC 
TCTGGACTGC ATCAGAATGG CCTTCACCAC GCACAGCCGT ACCATCCGCC TGGCACGCCC 
ACTCTGATGC ACAAGCGACT GGACTACCAC CAGCAGCCGC CGCCGGTGCC TCCGCACTCG 
GCCTATTACC AACAGGCACC CACGCACCAG CCGTCGCCGA TGATGGAGCG CAGTGTCAGG 
GGTCACCAGC AGCCCGGGGG CTTTCATATG GAGGAGGGCA CACCCACGCC GACCAGGATA 
CCATCCCTGC GGCGACAGCG ACGCACCTCG GGCAACCAGC AGGTGCACAA CAGCCACAGC 
AATCTCAATA TTGGCCTGAG CCACCACAAC AACAACAACA ACAACCTGCC GCCGCACCAC 
CAGCATCTGC ACCACCACCC CGGCCATCCG GGTGGCCTGG TGGGCATACC CATCGTGCCA 
GGGAGCCCTA GGGTCCAGAG ATCGCCGATG CCGAGCAGGG CGATGATCAA GAGGACGCGC 
CTGGGCAGCC ACACGGACAA CATATCGTCG GGCAGTCTGA ACAGTATTGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188540.1
CDS	436..3432
Translation	MAEIRLVVVLLVLMTTCIYTLQASDSNSSVATTLGVLFERAPESAVAPKGDEVVFECELNLKPDRLEWRFRRGDSSEPYLYLRPSAGYNVTSGSDGLAWRLRIYVSAQTAGDYQCVAWYGPGALASTPARLALVSIALDVGQGSMGARSSIRWSVAPKNCLLIRCGSVISNPPAIWSFYRNGKKLPQSELLPGAAGALVLDTVTAKDAGNYSCVATNSITGDELRLPQTIELRVDYTDRTPPYFLQRPPIEYSARPGETVVLECPGVGSPRPRVIWSSPNVVEIYNNRSTILSYGLQITDVKPEDQGSYICMLDNGIAPPIDHTIKLSVLQRPTILRGPAATLTNESNPLLLDCSASGNPQPDIYWLINGEDATKDPEAVVDNRSLQLKRVQKRHAGVVQCFAKNILGETSEGNILRVNPLEITGEDDEPLGEVPVWPVHESNTGMGTSSTSGGSKNKSGRRKFKASMVPPSRPNVTRLSDESVMLRWSVPQNSGLQITFFKVQYRMLGDGKNRRKNWQTTSDNIPYGNAHGSGPGQGNSHWRARNRDREYEMGNPPKNFTSSVTGLTAGKYYRFRIVAVYSNNDNKEGNTSLKFFLQNGTSKSNLPVPELREVETLSESAVVLHWSLVSSTQNEDIDGYYAYYRPADSAAEYLKATVDGGMSRSFKIDLLRPGTPYEFKLQSFNSDAASEFSAIRQARTRKTHEQPASSSATPVPANKAVEQHQNSLYPLIAGAAGGGILLLIASLVVCLCLKRRENSQPEDENKPQLEHIQADFVTSAVLGVGGHHKSGDVRRLNGVIPRMNITPNPLAQETASDKNNSGLHQNGLHHAQPYHPPGTPTLMHKRLDYHQQPPPVPPHSAYYQQAPTHQPSPMMERSVRGHQQPGGFHMEEGTPTPTRIPSLRRQRRTSGNQQVHNSHSNLNIGLSHHNNNNNNLPPHHQHLHHHPGHPGGLVGIPIVPGSPRVQRSPMPSRAMIKRTRLGSHTDNISSGSLNSIEV

Target ORF information:

RefSeq Version	NM_001201611.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brother of ihog (boi), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201611.2

ORF Insert Sequence:

ATGGCTGAAA TCAGGCTAGT TGTGGTTCTA CTGGTCCTTA TGACCACCTG TATCTATACA 
CTTCAAGCCA GTGACAGTAA TTCCTCCGTG GCAACCACCT TGGGCGTTTT GTTCGAGAGG 
GCTCCCGAAT CAGCGGTGGC CCCGAAAGGA GATGAGGTGG TCTTCGAGTG CGAGCTGAAT 
CTGAAACCTG ATCGCCTGGA GTGGCGGTTT AGACGTGGTG ATTCCTCGGA GCCCTATCTA 
TACCTAAGGC CCTCGGCTGG CTATAACGTG ACCAGCGGAA GCGATGGTCT TGCCTGGAGA 
CTGCGCATCT ATGTGAGTGC CCAAACGGCT GGGGACTACC AGTGTGTGGC TTGGTATGGC 
CCGGGTGCAC TGGCTTCCAC GCCAGCCCGC TTGGCCTTGG TCTCGATTGC TTTGGACGTC 
GGCCAGGGAT CCATGGGGGC CAGGAGCTCC ATCCGCTGGA GCGTGGCGCC CAAAAACTGC 
CTGCTCATTC GTTGCGGCAG CGTAATATCG AATCCGCCGG CTATTTGGAG CTTCTACAGA 
AACGGAAAGA AGCTACCTCA ATCGGAGCTG CTGCCTGGCG CTGCGGGGGC ATTGGTTTTG 
GATACGGTCA CCGCCAAAGA TGCGGGTAAC TACTCGTGCG TGGCCACGAA CTCCATAACA 
GGCGATGAAC TTAGGCTCCC CCAAACTATT GAACTAAGAG TGGACTACAC AGATAGGACA 
CCTCCGTACT TTCTACAACG TCCACCCATC GAATACTCAG CTCGTCCCGG AGAAACAGTG 
GTGCTCGAGT GTCCTGGCGT GGGATCCCCA CGACCCCGAG TCATTTGGAG TAGTCCCAAT 
GTGGTTGAGA TCTATAATAA TAGAAGCACC ATCCTGTCCT ACGGCCTGCA AATCACCGAT 
GTGAAGCCCG AGGATCAGGG TTCCTATATC TGCATGCTGG ACAATGGTAT TGCACCACCG 
ATCGATCACA CCATCAAGTT GAGTGTTTTG CAGCGGCCCA CGATCTTAAG AGGACCGGCC 
GCCACTCTGA CGAATGAGAG CAATCCTTTG CTGCTGGATT GCTCCGCCTC GGGGAATCCC 
CAACCCGATA TTTATTGGCT CATAAACGGT GAGGATGCCA CCAAAGATCC GGAGGCTGTG 
GTGGATAACC GGAGCCTGCA GCTGAAGCGA GTTCAAAAGC GTCACGCTGG CGTGGTTCAG 
TGCTTTGCAA AGAACATTTT GGGAGAGACG AGCGAGGGAA ACATATTGCG CGTGAATCCC 
CTGGAAATCA CCGGCGAGGA TGACGAACCA CTTGGCGAAG TTCCCGTGTG GCCAGTTCAT 
GAATCCAACA CTGGAATGGG CACCTCGTCA ACATCTGGAG GCTCCAAAAA CAAGAGCGGC 
AGACGAAAGT TTAAGGCGAG CATGGTGCCT CCGTCACGAC CCAATGTCAC CCGTCTGTCG 
GACGAGTCCG TGATGCTGCG CTGGAGTGTG CCACAAAACT CTGGACTCCA GATCACCTTT 
TTCAAGGTGC AGTATCGCAT GTTGGGCGAC GGAAAAAATC GGAGGAAGAA CTGGCAGACG 
ACCAGCGACA ATATCCCGTA CGGAAACGCG CACGGATCCG GGCCTGGCCA GGGGAACAGT 
CACTGGCGGG CGCGGAACCG GGACAGGGAG TATGAAATGG GCAATCCGCC CAAGAACTTC 
ACCTCCTCCG TTACGGGACT GACCGCCGGC AAGTACTACC GCTTCCGGAT CGTGGCGGTG 
TACTCCAATA ACGACAACAA GGAGGGCAAC ACCTCCCTCA AGTTCTTCCT GCAGAATGGA 
ACGTCCAAGT CGAATCTGCC AGTGCCGGAA CTACGCGAGG TGGAGACGCT TTCCGAGTCG 
GCCGTGGTGC TCCATTGGTC CCTGGTATCC TCGACTCAAA ATGAGGATAT AGACGGGTAC 
TATGCCTACT ATAGGCCAGC GGACTCAGCA GCGGAGTATT TGAAGGCCAC GGTGGATGGC 
GGAATGAGCA GGAGCTTCAA GATAGACCTC CTTCGTCCAG GAACCCCTTA TGAGTTCAAA 
TTGCAATCGT TCAACTCCGA CGCAGCCTCT GAATTCAGTG CTATTCGGCA AGCGAGGACC 
AGAAAAACGC ACGAGCAACC GGCTTCATCT TCGGCCACGC CAGTGCCAGC CAATAAGGCA 
GTGGAACAGC ACCAGAACTC CTTATATCCA CTGATCGCAG GTGCCGCTGG CGGAGGCATA 
CTACTCCTCA TCGCCTCATT GGTGGTCTGC CTTTGCCTAA AGAGACGGGA AAACTCTCAG 
CCGGAGGATG AGAACAAACC GCAATTGGAA CACATTCAAG CGGACTTTGT TACCTCCGCC 
GTCCTGGGAG TTGGTGGCCA CCACAAAAGT GGCGATGTAA GGCGCCTCAA CGGAGTGATT 
CCGAGGATGA ATATTACGCC CAATCCGCTG GCCCAGGAAA CGGCATCCGA TAAGAACAAC 
TCTGGACTGC ATCAGAATGG CCTTCACCAC GCACAGCCGT ACCATCCGCC TGGCACGCCC 
ACTCTGATGC ACAAGCGACT GGACTACCAC CAGCAGCCGC CGCCGGTGCC TCCGCACTCG 
GCCTATTACC AACAGGCACC CACGCACCAG CCGTCGCCGA TGATGGAGCG CAGTGTCAGG 
GGTCACCAGC AGCCCGGGGG CTTTCATATG GAGGAGGGCA CACCCACGCC GACCAGGATA 
CCATCCCTGC GGCGACAGCG ACGCACCTCG GGCAACCAGC AGGTGCACAA CAGCCACAGC 
AATCTCAATA TTGGCCTGAG CCACCACAAC AACAACAACA ACAACCTGCC GCCGCACCAC 
CAGCATCTGC ACCACCACCC CGGCCATCCG GGTGGCCTGG TGGGCATACC CATCGTGCCA 
GGGAGCCCTA GGGTCCAGAG ATCGCCGATG CCGAGCAGGG CGATGATCAA GAGGACGCGC 
CTGGGCAGCC ACACGGACAA CATATCGTCG GGCAGTCTGA ACAGTATTGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

boi ( NM_166940.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726811.1 Drosophila melanogaster brother of ihog (boi), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00605
Clone ID Related Accession (Same CDS sequence)	NM_001201611.2 , NM_166940.3 , NM_001201612.2
Accession Version	NM_166940.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brother of ihog, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_166940.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGAAA TCAGGCTAGT TGTGGTTCTA CTGGTCCTTA TGACCACCTG TATCTATACA 
CTTCAAGCCA GTGACAGTAA TTCCTCCGTG GCAACCACCT TGGGCGTTTT GTTCGAGAGG 
GCTCCCGAAT CAGCGGTGGC CCCGAAAGGA GATGAGGTGG TCTTCGAGTG CGAGCTGAAT 
CTGAAACCTG ATCGCCTGGA GTGGCGGTTT AGACGTGGTG ATTCCTCGGA GCCCTATCTA 
TACCTAAGGC CCTCGGCTGG CTATAACGTG ACCAGCGGAA GCGATGGTCT TGCCTGGAGA 
CTGCGCATCT ATGTGAGTGC CCAAACGGCT GGGGACTACC AGTGTGTGGC TTGGTATGGC 
CCGGGTGCAC TGGCTTCCAC GCCAGCCCGC TTGGCCTTGG TCTCGATTGC TTTGGACGTC 
GGCCAGGGAT CCATGGGGGC CAGGAGCTCC ATCCGCTGGA GCGTGGCGCC CAAAAACTGC 
CTGCTCATTC GTTGCGGCAG CGTAATATCG AATCCGCCGG CTATTTGGAG CTTCTACAGA 
AACGGAAAGA AGCTACCTCA ATCGGAGCTG CTGCCTGGCG CTGCGGGGGC ATTGGTTTTG 
GATACGGTCA CCGCCAAAGA TGCGGGTAAC TACTCGTGCG TGGCCACGAA CTCCATAACA 
GGCGATGAAC TTAGGCTCCC CCAAACTATT GAACTAAGAG TGGACTACAC AGATAGGACA 
CCTCCGTACT TTCTACAACG TCCACCCATC GAATACTCAG CTCGTCCCGG AGAAACAGTG 
GTGCTCGAGT GTCCTGGCGT GGGATCCCCA CGACCCCGAG TCATTTGGAG TAGTCCCAAT 
GTGGTTGAGA TCTATAATAA TAGAAGCACC ATCCTGTCCT ACGGCCTGCA AATCACCGAT 
GTGAAGCCCG AGGATCAGGG TTCCTATATC TGCATGCTGG ACAATGGTAT TGCACCACCG 
ATCGATCACA CCATCAAGTT GAGTGTTTTG CAGCGGCCCA CGATCTTAAG AGGACCGGCC 
GCCACTCTGA CGAATGAGAG CAATCCTTTG CTGCTGGATT GCTCCGCCTC GGGGAATCCC 
CAACCCGATA TTTATTGGCT CATAAACGGT GAGGATGCCA CCAAAGATCC GGAGGCTGTG 
GTGGATAACC GGAGCCTGCA GCTGAAGCGA GTTCAAAAGC GTCACGCTGG CGTGGTTCAG 
TGCTTTGCAA AGAACATTTT GGGAGAGACG AGCGAGGGAA ACATATTGCG CGTGAATCCC 
CTGGAAATCA CCGGCGAGGA TGACGAACCA CTTGGCGAAG TTCCCGTGTG GCCAGTTCAT 
GAATCCAACA CTGGAATGGG CACCTCGTCA ACATCTGGAG GCTCCAAAAA CAAGAGCGGC 
AGACGAAAGT TTAAGGCGAG CATGGTGCCT CCGTCACGAC CCAATGTCAC CCGTCTGTCG 
GACGAGTCCG TGATGCTGCG CTGGAGTGTG CCACAAAACT CTGGACTCCA GATCACCTTT 
TTCAAGGTGC AGTATCGCAT GTTGGGCGAC GGAAAAAATC GGAGGAAGAA CTGGCAGACG 
ACCAGCGACA ATATCCCGTA CGGAAACGCG CACGGATCCG GGCCTGGCCA GGGGAACAGT 
CACTGGCGGG CGCGGAACCG GGACAGGGAG TATGAAATGG GCAATCCGCC CAAGAACTTC 
ACCTCCTCCG TTACGGGACT GACCGCCGGC AAGTACTACC GCTTCCGGAT CGTGGCGGTG 
TACTCCAATA ACGACAACAA GGAGGGCAAC ACCTCCCTCA AGTTCTTCCT GCAGAATGGA 
ACGTCCAAGT CGAATCTGCC AGTGCCGGAA CTACGCGAGG TGGAGACGCT TTCCGAGTCG 
GCCGTGGTGC TCCATTGGTC CCTGGTATCC TCGACTCAAA ATGAGGATAT AGACGGGTAC 
TATGCCTACT ATAGGCCAGC GGACTCAGCA GCGGAGTATT TGAAGGCCAC GGTGGATGGC 
GGAATGAGCA GGAGCTTCAA GATAGACCTC CTTCGTCCAG GAACCCCTTA TGAGTTCAAA 
TTGCAATCGT TCAACTCCGA CGCAGCCTCT GAATTCAGTG CTATTCGGCA AGCGAGGACC 
AGAAAAACGC ACGAGCAACC GGCTTCATCT TCGGCCACGC CAGTGCCAGC CAATAAGGCA 
GTGGAACAGC ACCAGAACTC CTTATATCCA CTGATCGCAG GTGCCGCTGG CGGAGGCATA 
CTACTCCTCA TCGCCTCATT GGTGGTCTGC CTTTGCCTAA AGAGACGGGA AAACTCTCAG 
CCGGAGGATG AGAACAAACC GCAATTGGAA CACATTCAAG CGGACTTTGT TACCTCCGCC 
GTCCTGGGAG TTGGTGGCCA CCACAAAAGT GGCGATGTAA GGCGCCTCAA CGGAGTGATT 
CCGAGGATGA ATATTACGCC CAATCCGCTG GCCCAGGAAA CGGCATCCGA TAAGAACAAC 
TCTGGACTGC ATCAGAATGG CCTTCACCAC GCACAGCCGT ACCATCCGCC TGGCACGCCC 
ACTCTGATGC ACAAGCGACT GGACTACCAC CAGCAGCCGC CGCCGGTGCC TCCGCACTCG 
GCCTATTACC AACAGGCACC CACGCACCAG CCGTCGCCGA TGATGGAGCG CAGTGTCAGG 
GGTCACCAGC AGCCCGGGGG CTTTCATATG GAGGAGGGCA CACCCACGCC GACCAGGATA 
CCATCCCTGC GGCGACAGCG ACGCACCTCG GGCAACCAGC AGGTGCACAA CAGCCACAGC 
AATCTCAATA TTGGCCTGAG CCACCACAAC AACAACAACA ACAACCTGCC GCCGCACCAC 
CAGCATCTGC ACCACCACCC CGGCCATCCG GGTGGCCTGG TGGGCATACC CATCGTGCCA 
GGGAGCCCTA GGGTCCAGAG ATCGCCGATG CCGAGCAGGG CGATGATCAA GAGGACGCGC 
CTGGGCAGCC ACACGGACAA CATATCGTCG GGCAGTCTGA ACAGTATTGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726811.1
CDS	343..3339
Translation	MAEIRLVVVLLVLMTTCIYTLQASDSNSSVATTLGVLFERAPESAVAPKGDEVVFECELNLKPDRLEWRFRRGDSSEPYLYLRPSAGYNVTSGSDGLAWRLRIYVSAQTAGDYQCVAWYGPGALASTPARLALVSIALDVGQGSMGARSSIRWSVAPKNCLLIRCGSVISNPPAIWSFYRNGKKLPQSELLPGAAGALVLDTVTAKDAGNYSCVATNSITGDELRLPQTIELRVDYTDRTPPYFLQRPPIEYSARPGETVVLECPGVGSPRPRVIWSSPNVVEIYNNRSTILSYGLQITDVKPEDQGSYICMLDNGIAPPIDHTIKLSVLQRPTILRGPAATLTNESNPLLLDCSASGNPQPDIYWLINGEDATKDPEAVVDNRSLQLKRVQKRHAGVVQCFAKNILGETSEGNILRVNPLEITGEDDEPLGEVPVWPVHESNTGMGTSSTSGGSKNKSGRRKFKASMVPPSRPNVTRLSDESVMLRWSVPQNSGLQITFFKVQYRMLGDGKNRRKNWQTTSDNIPYGNAHGSGPGQGNSHWRARNRDREYEMGNPPKNFTSSVTGLTAGKYYRFRIVAVYSNNDNKEGNTSLKFFLQNGTSKSNLPVPELREVETLSESAVVLHWSLVSSTQNEDIDGYYAYYRPADSAAEYLKATVDGGMSRSFKIDLLRPGTPYEFKLQSFNSDAASEFSAIRQARTRKTHEQPASSSATPVPANKAVEQHQNSLYPLIAGAAGGGILLLIASLVVCLCLKRRENSQPEDENKPQLEHIQADFVTSAVLGVGGHHKSGDVRRLNGVIPRMNITPNPLAQETASDKNNSGLHQNGLHHAQPYHPPGTPTLMHKRLDYHQQPPPVPPHSAYYQQAPTHQPSPMMERSVRGHQQPGGFHMEEGTPTPTRIPSLRRQRRTSGNQQVHNSHSNLNIGLSHHNNNNNNLPPHHQHLHHHPGHPGGLVGIPIVPGSPRVQRSPMPSRAMIKRTRLGSHTDNISSGSLNSIEV

Target ORF information:

RefSeq Version	NM_166940.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brother of ihog (boi), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166940.3

ORF Insert Sequence:

ATGGCTGAAA TCAGGCTAGT TGTGGTTCTA CTGGTCCTTA TGACCACCTG TATCTATACA 
CTTCAAGCCA GTGACAGTAA TTCCTCCGTG GCAACCACCT TGGGCGTTTT GTTCGAGAGG 
GCTCCCGAAT CAGCGGTGGC CCCGAAAGGA GATGAGGTGG TCTTCGAGTG CGAGCTGAAT 
CTGAAACCTG ATCGCCTGGA GTGGCGGTTT AGACGTGGTG ATTCCTCGGA GCCCTATCTA 
TACCTAAGGC CCTCGGCTGG CTATAACGTG ACCAGCGGAA GCGATGGTCT TGCCTGGAGA 
CTGCGCATCT ATGTGAGTGC CCAAACGGCT GGGGACTACC AGTGTGTGGC TTGGTATGGC 
CCGGGTGCAC TGGCTTCCAC GCCAGCCCGC TTGGCCTTGG TCTCGATTGC TTTGGACGTC 
GGCCAGGGAT CCATGGGGGC CAGGAGCTCC ATCCGCTGGA GCGTGGCGCC CAAAAACTGC 
CTGCTCATTC GTTGCGGCAG CGTAATATCG AATCCGCCGG CTATTTGGAG CTTCTACAGA 
AACGGAAAGA AGCTACCTCA ATCGGAGCTG CTGCCTGGCG CTGCGGGGGC ATTGGTTTTG 
GATACGGTCA CCGCCAAAGA TGCGGGTAAC TACTCGTGCG TGGCCACGAA CTCCATAACA 
GGCGATGAAC TTAGGCTCCC CCAAACTATT GAACTAAGAG TGGACTACAC AGATAGGACA 
CCTCCGTACT TTCTACAACG TCCACCCATC GAATACTCAG CTCGTCCCGG AGAAACAGTG 
GTGCTCGAGT GTCCTGGCGT GGGATCCCCA CGACCCCGAG TCATTTGGAG TAGTCCCAAT 
GTGGTTGAGA TCTATAATAA TAGAAGCACC ATCCTGTCCT ACGGCCTGCA AATCACCGAT 
GTGAAGCCCG AGGATCAGGG TTCCTATATC TGCATGCTGG ACAATGGTAT TGCACCACCG 
ATCGATCACA CCATCAAGTT GAGTGTTTTG CAGCGGCCCA CGATCTTAAG AGGACCGGCC 
GCCACTCTGA CGAATGAGAG CAATCCTTTG CTGCTGGATT GCTCCGCCTC GGGGAATCCC 
CAACCCGATA TTTATTGGCT CATAAACGGT GAGGATGCCA CCAAAGATCC GGAGGCTGTG 
GTGGATAACC GGAGCCTGCA GCTGAAGCGA GTTCAAAAGC GTCACGCTGG CGTGGTTCAG 
TGCTTTGCAA AGAACATTTT GGGAGAGACG AGCGAGGGAA ACATATTGCG CGTGAATCCC 
CTGGAAATCA CCGGCGAGGA TGACGAACCA CTTGGCGAAG TTCCCGTGTG GCCAGTTCAT 
GAATCCAACA CTGGAATGGG CACCTCGTCA ACATCTGGAG GCTCCAAAAA CAAGAGCGGC 
AGACGAAAGT TTAAGGCGAG CATGGTGCCT CCGTCACGAC CCAATGTCAC CCGTCTGTCG 
GACGAGTCCG TGATGCTGCG CTGGAGTGTG CCACAAAACT CTGGACTCCA GATCACCTTT 
TTCAAGGTGC AGTATCGCAT GTTGGGCGAC GGAAAAAATC GGAGGAAGAA CTGGCAGACG 
ACCAGCGACA ATATCCCGTA CGGAAACGCG CACGGATCCG GGCCTGGCCA GGGGAACAGT 
CACTGGCGGG CGCGGAACCG GGACAGGGAG TATGAAATGG GCAATCCGCC CAAGAACTTC 
ACCTCCTCCG TTACGGGACT GACCGCCGGC AAGTACTACC GCTTCCGGAT CGTGGCGGTG 
TACTCCAATA ACGACAACAA GGAGGGCAAC ACCTCCCTCA AGTTCTTCCT GCAGAATGGA 
ACGTCCAAGT CGAATCTGCC AGTGCCGGAA CTACGCGAGG TGGAGACGCT TTCCGAGTCG 
GCCGTGGTGC TCCATTGGTC CCTGGTATCC TCGACTCAAA ATGAGGATAT AGACGGGTAC 
TATGCCTACT ATAGGCCAGC GGACTCAGCA GCGGAGTATT TGAAGGCCAC GGTGGATGGC 
GGAATGAGCA GGAGCTTCAA GATAGACCTC CTTCGTCCAG GAACCCCTTA TGAGTTCAAA 
TTGCAATCGT TCAACTCCGA CGCAGCCTCT GAATTCAGTG CTATTCGGCA AGCGAGGACC 
AGAAAAACGC ACGAGCAACC GGCTTCATCT TCGGCCACGC CAGTGCCAGC CAATAAGGCA 
GTGGAACAGC ACCAGAACTC CTTATATCCA CTGATCGCAG GTGCCGCTGG CGGAGGCATA 
CTACTCCTCA TCGCCTCATT GGTGGTCTGC CTTTGCCTAA AGAGACGGGA AAACTCTCAG 
CCGGAGGATG AGAACAAACC GCAATTGGAA CACATTCAAG CGGACTTTGT TACCTCCGCC 
GTCCTGGGAG TTGGTGGCCA CCACAAAAGT GGCGATGTAA GGCGCCTCAA CGGAGTGATT 
CCGAGGATGA ATATTACGCC CAATCCGCTG GCCCAGGAAA CGGCATCCGA TAAGAACAAC 
TCTGGACTGC ATCAGAATGG CCTTCACCAC GCACAGCCGT ACCATCCGCC TGGCACGCCC 
ACTCTGATGC ACAAGCGACT GGACTACCAC CAGCAGCCGC CGCCGGTGCC TCCGCACTCG 
GCCTATTACC AACAGGCACC CACGCACCAG CCGTCGCCGA TGATGGAGCG CAGTGTCAGG 
GGTCACCAGC AGCCCGGGGG CTTTCATATG GAGGAGGGCA CACCCACGCC GACCAGGATA 
CCATCCCTGC GGCGACAGCG ACGCACCTCG GGCAACCAGC AGGTGCACAA CAGCCACAGC 
AATCTCAATA TTGGCCTGAG CCACCACAAC AACAACAACA ACAACCTGCC GCCGCACCAC 
CAGCATCTGC ACCACCACCC CGGCCATCCG GGTGGCCTGG TGGGCATACC CATCGTGCCA 
GGGAGCCCTA GGGTCCAGAG ATCGCCGATG CCGAGCAGGG CGATGATCAA GAGGACGCGC 
CTGGGCAGCC ACACGGACAA CATATCGTCG GGCAGTCTGA ACAGTATTGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

boi ( NM_001201612.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188541.1 Drosophila melanogaster brother of ihog (boi), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa00605
Clone ID Related Accession (Same CDS sequence)	NM_001201611.2 , NM_166940.3 , NM_001201612.2
Accession Version	NM_001201612.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brother of ihog, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001201612.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGAAA TCAGGCTAGT TGTGGTTCTA CTGGTCCTTA TGACCACCTG TATCTATACA 
CTTCAAGCCA GTGACAGTAA TTCCTCCGTG GCAACCACCT TGGGCGTTTT GTTCGAGAGG 
GCTCCCGAAT CAGCGGTGGC CCCGAAAGGA GATGAGGTGG TCTTCGAGTG CGAGCTGAAT 
CTGAAACCTG ATCGCCTGGA GTGGCGGTTT AGACGTGGTG ATTCCTCGGA GCCCTATCTA 
TACCTAAGGC CCTCGGCTGG CTATAACGTG ACCAGCGGAA GCGATGGTCT TGCCTGGAGA 
CTGCGCATCT ATGTGAGTGC CCAAACGGCT GGGGACTACC AGTGTGTGGC TTGGTATGGC 
CCGGGTGCAC TGGCTTCCAC GCCAGCCCGC TTGGCCTTGG TCTCGATTGC TTTGGACGTC 
GGCCAGGGAT CCATGGGGGC CAGGAGCTCC ATCCGCTGGA GCGTGGCGCC CAAAAACTGC 
CTGCTCATTC GTTGCGGCAG CGTAATATCG AATCCGCCGG CTATTTGGAG CTTCTACAGA 
AACGGAAAGA AGCTACCTCA ATCGGAGCTG CTGCCTGGCG CTGCGGGGGC ATTGGTTTTG 
GATACGGTCA CCGCCAAAGA TGCGGGTAAC TACTCGTGCG TGGCCACGAA CTCCATAACA 
GGCGATGAAC TTAGGCTCCC CCAAACTATT GAACTAAGAG TGGACTACAC AGATAGGACA 
CCTCCGTACT TTCTACAACG TCCACCCATC GAATACTCAG CTCGTCCCGG AGAAACAGTG 
GTGCTCGAGT GTCCTGGCGT GGGATCCCCA CGACCCCGAG TCATTTGGAG TAGTCCCAAT 
GTGGTTGAGA TCTATAATAA TAGAAGCACC ATCCTGTCCT ACGGCCTGCA AATCACCGAT 
GTGAAGCCCG AGGATCAGGG TTCCTATATC TGCATGCTGG ACAATGGTAT TGCACCACCG 
ATCGATCACA CCATCAAGTT GAGTGTTTTG CAGCGGCCCA CGATCTTAAG AGGACCGGCC 
GCCACTCTGA CGAATGAGAG CAATCCTTTG CTGCTGGATT GCTCCGCCTC GGGGAATCCC 
CAACCCGATA TTTATTGGCT CATAAACGGT GAGGATGCCA CCAAAGATCC GGAGGCTGTG 
GTGGATAACC GGAGCCTGCA GCTGAAGCGA GTTCAAAAGC GTCACGCTGG CGTGGTTCAG 
TGCTTTGCAA AGAACATTTT GGGAGAGACG AGCGAGGGAA ACATATTGCG CGTGAATCCC 
CTGGAAATCA CCGGCGAGGA TGACGAACCA CTTGGCGAAG TTCCCGTGTG GCCAGTTCAT 
GAATCCAACA CTGGAATGGG CACCTCGTCA ACATCTGGAG GCTCCAAAAA CAAGAGCGGC 
AGACGAAAGT TTAAGGCGAG CATGGTGCCT CCGTCACGAC CCAATGTCAC CCGTCTGTCG 
GACGAGTCCG TGATGCTGCG CTGGAGTGTG CCACAAAACT CTGGACTCCA GATCACCTTT 
TTCAAGGTGC AGTATCGCAT GTTGGGCGAC GGAAAAAATC GGAGGAAGAA CTGGCAGACG 
ACCAGCGACA ATATCCCGTA CGGAAACGCG CACGGATCCG GGCCTGGCCA GGGGAACAGT 
CACTGGCGGG CGCGGAACCG GGACAGGGAG TATGAAATGG GCAATCCGCC CAAGAACTTC 
ACCTCCTCCG TTACGGGACT GACCGCCGGC AAGTACTACC GCTTCCGGAT CGTGGCGGTG 
TACTCCAATA ACGACAACAA GGAGGGCAAC ACCTCCCTCA AGTTCTTCCT GCAGAATGGA 
ACGTCCAAGT CGAATCTGCC AGTGCCGGAA CTACGCGAGG TGGAGACGCT TTCCGAGTCG 
GCCGTGGTGC TCCATTGGTC CCTGGTATCC TCGACTCAAA ATGAGGATAT AGACGGGTAC 
TATGCCTACT ATAGGCCAGC GGACTCAGCA GCGGAGTATT TGAAGGCCAC GGTGGATGGC 
GGAATGAGCA GGAGCTTCAA GATAGACCTC CTTCGTCCAG GAACCCCTTA TGAGTTCAAA 
TTGCAATCGT TCAACTCCGA CGCAGCCTCT GAATTCAGTG CTATTCGGCA AGCGAGGACC 
AGAAAAACGC ACGAGCAACC GGCTTCATCT TCGGCCACGC CAGTGCCAGC CAATAAGGCA 
GTGGAACAGC ACCAGAACTC CTTATATCCA CTGATCGCAG GTGCCGCTGG CGGAGGCATA 
CTACTCCTCA TCGCCTCATT GGTGGTCTGC CTTTGCCTAA AGAGACGGGA AAACTCTCAG 
CCGGAGGATG AGAACAAACC GCAATTGGAA CACATTCAAG CGGACTTTGT TACCTCCGCC 
GTCCTGGGAG TTGGTGGCCA CCACAAAAGT GGCGATGTAA GGCGCCTCAA CGGAGTGATT 
CCGAGGATGA ATATTACGCC CAATCCGCTG GCCCAGGAAA CGGCATCCGA TAAGAACAAC 
TCTGGACTGC ATCAGAATGG CCTTCACCAC GCACAGCCGT ACCATCCGCC TGGCACGCCC 
ACTCTGATGC ACAAGCGACT GGACTACCAC CAGCAGCCGC CGCCGGTGCC TCCGCACTCG 
GCCTATTACC AACAGGCACC CACGCACCAG CCGTCGCCGA TGATGGAGCG CAGTGTCAGG 
GGTCACCAGC AGCCCGGGGG CTTTCATATG GAGGAGGGCA CACCCACGCC GACCAGGATA 
CCATCCCTGC GGCGACAGCG ACGCACCTCG GGCAACCAGC AGGTGCACAA CAGCCACAGC 
AATCTCAATA TTGGCCTGAG CCACCACAAC AACAACAACA ACAACCTGCC GCCGCACCAC 
CAGCATCTGC ACCACCACCC CGGCCATCCG GGTGGCCTGG TGGGCATACC CATCGTGCCA 
GGGAGCCCTA GGGTCCAGAG ATCGCCGATG CCGAGCAGGG CGATGATCAA GAGGACGCGC 
CTGGGCAGCC ACACGGACAA CATATCGTCG GGCAGTCTGA ACAGTATTGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188541.1
CDS	629..3625
Translation	MAEIRLVVVLLVLMTTCIYTLQASDSNSSVATTLGVLFERAPESAVAPKGDEVVFECELNLKPDRLEWRFRRGDSSEPYLYLRPSAGYNVTSGSDGLAWRLRIYVSAQTAGDYQCVAWYGPGALASTPARLALVSIALDVGQGSMGARSSIRWSVAPKNCLLIRCGSVISNPPAIWSFYRNGKKLPQSELLPGAAGALVLDTVTAKDAGNYSCVATNSITGDELRLPQTIELRVDYTDRTPPYFLQRPPIEYSARPGETVVLECPGVGSPRPRVIWSSPNVVEIYNNRSTILSYGLQITDVKPEDQGSYICMLDNGIAPPIDHTIKLSVLQRPTILRGPAATLTNESNPLLLDCSASGNPQPDIYWLINGEDATKDPEAVVDNRSLQLKRVQKRHAGVVQCFAKNILGETSEGNILRVNPLEITGEDDEPLGEVPVWPVHESNTGMGTSSTSGGSKNKSGRRKFKASMVPPSRPNVTRLSDESVMLRWSVPQNSGLQITFFKVQYRMLGDGKNRRKNWQTTSDNIPYGNAHGSGPGQGNSHWRARNRDREYEMGNPPKNFTSSVTGLTAGKYYRFRIVAVYSNNDNKEGNTSLKFFLQNGTSKSNLPVPELREVETLSESAVVLHWSLVSSTQNEDIDGYYAYYRPADSAAEYLKATVDGGMSRSFKIDLLRPGTPYEFKLQSFNSDAASEFSAIRQARTRKTHEQPASSSATPVPANKAVEQHQNSLYPLIAGAAGGGILLLIASLVVCLCLKRRENSQPEDENKPQLEHIQADFVTSAVLGVGGHHKSGDVRRLNGVIPRMNITPNPLAQETASDKNNSGLHQNGLHHAQPYHPPGTPTLMHKRLDYHQQPPPVPPHSAYYQQAPTHQPSPMMERSVRGHQQPGGFHMEEGTPTPTRIPSLRRQRRTSGNQQVHNSHSNLNIGLSHHNNNNNNLPPHHQHLHHHPGHPGGLVGIPIVPGSPRVQRSPMPSRAMIKRTRLGSHTDNISSGSLNSIEV

Target ORF information:

RefSeq Version	NM_001201612.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brother of ihog (boi), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201612.2

ORF Insert Sequence:

ATGGCTGAAA TCAGGCTAGT TGTGGTTCTA CTGGTCCTTA TGACCACCTG TATCTATACA 
CTTCAAGCCA GTGACAGTAA TTCCTCCGTG GCAACCACCT TGGGCGTTTT GTTCGAGAGG 
GCTCCCGAAT CAGCGGTGGC CCCGAAAGGA GATGAGGTGG TCTTCGAGTG CGAGCTGAAT 
CTGAAACCTG ATCGCCTGGA GTGGCGGTTT AGACGTGGTG ATTCCTCGGA GCCCTATCTA 
TACCTAAGGC CCTCGGCTGG CTATAACGTG ACCAGCGGAA GCGATGGTCT TGCCTGGAGA 
CTGCGCATCT ATGTGAGTGC CCAAACGGCT GGGGACTACC AGTGTGTGGC TTGGTATGGC 
CCGGGTGCAC TGGCTTCCAC GCCAGCCCGC TTGGCCTTGG TCTCGATTGC TTTGGACGTC 
GGCCAGGGAT CCATGGGGGC CAGGAGCTCC ATCCGCTGGA GCGTGGCGCC CAAAAACTGC 
CTGCTCATTC GTTGCGGCAG CGTAATATCG AATCCGCCGG CTATTTGGAG CTTCTACAGA 
AACGGAAAGA AGCTACCTCA ATCGGAGCTG CTGCCTGGCG CTGCGGGGGC ATTGGTTTTG 
GATACGGTCA CCGCCAAAGA TGCGGGTAAC TACTCGTGCG TGGCCACGAA CTCCATAACA 
GGCGATGAAC TTAGGCTCCC CCAAACTATT GAACTAAGAG TGGACTACAC AGATAGGACA 
CCTCCGTACT TTCTACAACG TCCACCCATC GAATACTCAG CTCGTCCCGG AGAAACAGTG 
GTGCTCGAGT GTCCTGGCGT GGGATCCCCA CGACCCCGAG TCATTTGGAG TAGTCCCAAT 
GTGGTTGAGA TCTATAATAA TAGAAGCACC ATCCTGTCCT ACGGCCTGCA AATCACCGAT 
GTGAAGCCCG AGGATCAGGG TTCCTATATC TGCATGCTGG ACAATGGTAT TGCACCACCG 
ATCGATCACA CCATCAAGTT GAGTGTTTTG CAGCGGCCCA CGATCTTAAG AGGACCGGCC 
GCCACTCTGA CGAATGAGAG CAATCCTTTG CTGCTGGATT GCTCCGCCTC GGGGAATCCC 
CAACCCGATA TTTATTGGCT CATAAACGGT GAGGATGCCA CCAAAGATCC GGAGGCTGTG 
GTGGATAACC GGAGCCTGCA GCTGAAGCGA GTTCAAAAGC GTCACGCTGG CGTGGTTCAG 
TGCTTTGCAA AGAACATTTT GGGAGAGACG AGCGAGGGAA ACATATTGCG CGTGAATCCC 
CTGGAAATCA CCGGCGAGGA TGACGAACCA CTTGGCGAAG TTCCCGTGTG GCCAGTTCAT 
GAATCCAACA CTGGAATGGG CACCTCGTCA ACATCTGGAG GCTCCAAAAA CAAGAGCGGC 
AGACGAAAGT TTAAGGCGAG CATGGTGCCT CCGTCACGAC CCAATGTCAC CCGTCTGTCG 
GACGAGTCCG TGATGCTGCG CTGGAGTGTG CCACAAAACT CTGGACTCCA GATCACCTTT 
TTCAAGGTGC AGTATCGCAT GTTGGGCGAC GGAAAAAATC GGAGGAAGAA CTGGCAGACG 
ACCAGCGACA ATATCCCGTA CGGAAACGCG CACGGATCCG GGCCTGGCCA GGGGAACAGT 
CACTGGCGGG CGCGGAACCG GGACAGGGAG TATGAAATGG GCAATCCGCC CAAGAACTTC 
ACCTCCTCCG TTACGGGACT GACCGCCGGC AAGTACTACC GCTTCCGGAT CGTGGCGGTG 
TACTCCAATA ACGACAACAA GGAGGGCAAC ACCTCCCTCA AGTTCTTCCT GCAGAATGGA 
ACGTCCAAGT CGAATCTGCC AGTGCCGGAA CTACGCGAGG TGGAGACGCT TTCCGAGTCG 
GCCGTGGTGC TCCATTGGTC CCTGGTATCC TCGACTCAAA ATGAGGATAT AGACGGGTAC 
TATGCCTACT ATAGGCCAGC GGACTCAGCA GCGGAGTATT TGAAGGCCAC GGTGGATGGC 
GGAATGAGCA GGAGCTTCAA GATAGACCTC CTTCGTCCAG GAACCCCTTA TGAGTTCAAA 
TTGCAATCGT TCAACTCCGA CGCAGCCTCT GAATTCAGTG CTATTCGGCA AGCGAGGACC 
AGAAAAACGC ACGAGCAACC GGCTTCATCT TCGGCCACGC CAGTGCCAGC CAATAAGGCA 
GTGGAACAGC ACCAGAACTC CTTATATCCA CTGATCGCAG GTGCCGCTGG CGGAGGCATA 
CTACTCCTCA TCGCCTCATT GGTGGTCTGC CTTTGCCTAA AGAGACGGGA AAACTCTCAG 
CCGGAGGATG AGAACAAACC GCAATTGGAA CACATTCAAG CGGACTTTGT TACCTCCGCC 
GTCCTGGGAG TTGGTGGCCA CCACAAAAGT GGCGATGTAA GGCGCCTCAA CGGAGTGATT 
CCGAGGATGA ATATTACGCC CAATCCGCTG GCCCAGGAAA CGGCATCCGA TAAGAACAAC 
TCTGGACTGC ATCAGAATGG CCTTCACCAC GCACAGCCGT ACCATCCGCC TGGCACGCCC 
ACTCTGATGC ACAAGCGACT GGACTACCAC CAGCAGCCGC CGCCGGTGCC TCCGCACTCG 
GCCTATTACC AACAGGCACC CACGCACCAG CCGTCGCCGA TGATGGAGCG CAGTGTCAGG 
GGTCACCAGC AGCCCGGGGG CTTTCATATG GAGGAGGGCA CACCCACGCC GACCAGGATA 
CCATCCCTGC GGCGACAGCG ACGCACCTCG GGCAACCAGC AGGTGCACAA CAGCCACAGC 
AATCTCAATA TTGGCCTGAG CCACCACAAC AACAACAACA ACAACCTGCC GCCGCACCAC 
CAGCATCTGC ACCACCACCC CGGCCATCCG GGTGGCCTGG TGGGCATACC CATCGTGCCA 
GGGAGCCCTA GGGTCCAGAG ATCGCCGATG CCGAGCAGGG CGATGATCAA GAGGACGCGC 
CTGGGCAGCC ACACGGACAA CATATCGTCG GGCAGTCTGA ACAGTATTGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

boi ( NM_001103398.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096868.3 Drosophila melanogaster brother of ihog (boi), transcript variant G, mRNA.

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CloneID	OFa00606
Clone ID Related Accession (Same CDS sequence)	NM_001103398.4
Accession Version	NM_001103398.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brother of ihog, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001103398.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGAAA TCAGGCTAGT TGTGGTTCTA CTGGTCCTTA TGACCACCTG TATCTATACA 
CTTCAAGCCA GTGACAGTAA TTCCTCCGTG GCAACCACCT TGGGCGTTTT GTTCGAGAGG 
GCTCCCGAAT CAGCGGTGGC CCCGAAAGGA GATGAGGTGG TCTTCGAGTG CGAGCTGAAT 
CTGAAACCTG ATCGCCTGGA GTGGCGGTTT AGACGTGGTG ATTCCTCGGA GCCCTATCTA 
TACCTAAGGC CCTCGGCTGG CTATAACGTG ACCAGCGGAA GCGATGGTCT TGCCTGGAGA 
CTGCGCATCT ATGTGAGTGC CCAAACGGCT GGGGACTACC AGTGTGTGGC TTGGTATGGC 
CCGGGTGCAC TGGCTTCCAC GCCAGCCCGC TTGGCCTTGG TCTCGATTGC TTTGGACGTC 
GGCCAGGGAT CCATGGGGGC CAGGAGCTCC ATCCGCTGGA GCGTGGCGCC CAAAAACTGC 
CTGCTCATTC GTTGCGGCAG CGTAATATCG AATCCGCCGG CTATTTGGAG CTTCTACAGA 
AACGGAAAGA AGCTACCTCA ATCGGAGCTG CTGCCTGGCG CTGCGGGGGC ATTGGTTTTG 
GATACGGTCA CCGCCAAAGA TGCGGGTAAC TACTCGTGCG TGGCCACGAA CTCCATAACA 
GGCGATGAAC TTAGGCTCCC CCAAACTATT GAACTAAGAG TGGACTACAC AGATAGGACA 
CCTCCGTACT TTCTACAACG TCCACCCATC GAATACTCAG CTCGTCCCGG AGAAACAGTG 
GTGCTCGAGT GTCCTGGCGT GGGATCCCCA CGACCCCGAG TCATTTGGAG TAGTCCCAAT 
GTGGTTGAGA TCTATAATAA TAGAAGCACC ATCCTGTCCT ACGGCCTGCA AATCACCGAT 
GTGAAGCCCG AGGATCAGGG TTCCTATATC TGCATGCTGG ACAATGGTAT TGCACCACCG 
ATCGATCACA CCATCAAGTT GAGTGTTTTG CAGCGGCCCA CGATCTTAAG AGGACCGGCC 
GCCACTCTGA CGAATGAGAG CAATCCTTTG CTGCTGGATT GCTCCGCCTC GGGGAATCCC 
CAACCCGATA TTTATTGGCT CATAAACGGT GAGGATGCCA CCAAAGATCC GGAGGCTGTG 
GTGGATAACC GGAGCCTGCA GCTGAAGCGA GTTCAAAAGC GTCACGCTGG CGTGGTTCAG 
TGCTTTGCAA AGAACATTTT GGGAGAGACG AGCGAGGGAA ACATATTGCG CGTGAATCCC 
CTGGAAATCA CCGGCGAGGA TGACGAACCA CTTGGCGAAG TTCCCGTGTG GCCAGTTCAT 
GAATCCAACA CTGGAATGGG CACCTCGTCA ACATCTGGAG GCTCCAAAAA CAAGAGCGGC 
AGACGAAAGT TTAAGGCGAG CATGGTGCCT CCGTCACGAC CCAATGTCAC CCGTCTGTCG 
GACGAGTCCG TGATGCTGCG CTGGAGTGTG CCACAAAACT CTGGACTCCA GATCACCTTT 
TTCAAGGTGC AGTATCGCAT GTTGGGCGAC GGAAAAAATC GGAGGAAGAA CTGGCAGACG 
ACCAGCGACA ATATCCCGTA CGGAAACGCG CACGGATCCG GGCCTGGCCA GGGGAACAGT 
CACTGGCGGG CGCGGAACCG GGACAGGGAG TATGAAATGG GCAATCCGCC CAAGAACTTC 
ACCTCCTCCG TTACGGGACT GACCGCCGGC AAGTACTACC GCTTCCGGAT CGTGGCGGTG 
TACTCCAATA ACGACAACAA GGAGGGCAAC ACCTCCCTCA AGTTCTTCCT GCAGAATGGA 
ACGTCCAAGT CGAATCTGCC AGTGCCGGAA CTACGCGAGG TGGAGACGCT TTCCGAGTCG 
GCCGTGGTGC TCCATTGGTC CCTGGTATCC TCGACTCAAA ATGAGGATAT AGACGGGTAC 
TATGCCTACT ATAGGCCAGC GGACTCAGCA GCGGAGTATT TGAAGGCCAC GGTGGATGGC 
GGAATGAGCA GGAGCTTCAA GATAGACCTC CTTCGTCCAG GAACCCCTTA TGAGTTCAAA 
TTGCAATCGT TCAACTCCGA CGCAGCCTCT GAATTCAGTG CTATTCGGCA AGCGAGGACC 
AGAAAAACGC ACGAGCAACC GGCTTCATCT TCGGCCACGC CAGTGCCAGC CAATAAGGCA 
GTGGAACAGC ACCAGAACTC CTTATATCCA CTGATCGCAG GTGCCGCTGG CGGAGGCATA 
CTACTCCTCA TCGCCTCATT GGTGGTCTGC CTTTGCCTAA AGAGACGGGA AAACTCTCAG 
CCGGAGGATG AGAACAAACC GCAATTGGAA CACATTCAAG CGGACTTTGT TACCTCCGCC 
GTCCTGGGAG TTGGTGGCCA CCACAAAAGT GGCGATGTAA GGCGCCTCAA CGGAGTGATT 
CCGAGGATGA ATATTACGCC CAATCCGCTG GCCCAGGAAA CGGCATCCGA TAAGAACCGC 
AACGTGATGG AGCTGCGTTT CCTGCCGCCG CTGGCGAATG GAAATGGAAA TGGCAACTGC 
ATGGCCAGAG ATCTAGCCCT GGAGCGGTCG AACGCGGAAG AGCAGCAGGA TGAGGACGGA 
CACGAAGATG AAAAGGGGCT ACCGGCGGTT GCCTCGTCCT CCTGCGAAAC GCTGGAGGCC 
TGCGACGCTG CAATTAAACT GAATCTCCCT CAGAAACACG ACCAGGAGCA GCTGGATGCT 
CCGAACGCGG TGCTGGAGGC GCCCAGCAAG CCGCTACCTC CGCTGCCCAA GAAACCAGCA 
AACGGCGCTG GCCAAAACAA CTCTGGACTG CATCAGAATG GCCTTCACCA CGCACAGCCG 
TACCATCCGC CTGGCACGCC CACTCTGATG CACAAGCGAC TGGACTACCA CCAGCAGCCG 
CCGCCGGTGC CTCCGCACTC GGCCTATTAC CAACAGGCAC CCACGCACCA GCCGTCGCCG 
ATGATGGAGC GCAGTGTCAG GGGTCACCAG CAGCCCGGGG GCTTTCATAT GGAGGAGGGC 
ACACCCACGC CGACCAGGAT ACCATCCCTG CGGCGACAGC GACGCACCTC GGGCAACCAG 
CAGGTGCACA ACAGCCACAG CAATCTCAAT ATTGGCCTGA GCCACCACAA CAACAACAAC 
AACAACCTGC CGCCGCACCA CCAGCATCTG CACCACCACC CCGGCCATCC GGGTGGCCTG 
GTGGGCATAC CCATCGTGCC AGGGAGCCCT AGGGTCCAGA GATCGCCGAT GCCGAGCAGG 
GCGATGATCA AGAGGACGCG CCTGGGCAGC CACACGGACA ACATATCGTC GGGCAGTCTG 
AACAGTATTG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096868.3
CDS	343..3660
Translation	MAEIRLVVVLLVLMTTCIYTLQASDSNSSVATTLGVLFERAPESAVAPKGDEVVFECELNLKPDRLEWRFRRGDSSEPYLYLRPSAGYNVTSGSDGLAWRLRIYVSAQTAGDYQCVAWYGPGALASTPARLALVSIALDVGQGSMGARSSIRWSVAPKNCLLIRCGSVISNPPAIWSFYRNGKKLPQSELLPGAAGALVLDTVTAKDAGNYSCVATNSITGDELRLPQTIELRVDYTDRTPPYFLQRPPIEYSARPGETVVLECPGVGSPRPRVIWSSPNVVEIYNNRSTILSYGLQITDVKPEDQGSYICMLDNGIAPPIDHTIKLSVLQRPTILRGPAATLTNESNPLLLDCSASGNPQPDIYWLINGEDATKDPEAVVDNRSLQLKRVQKRHAGVVQCFAKNILGETSEGNILRVNPLEITGEDDEPLGEVPVWPVHESNTGMGTSSTSGGSKNKSGRRKFKASMVPPSRPNVTRLSDESVMLRWSVPQNSGLQITFFKVQYRMLGDGKNRRKNWQTTSDNIPYGNAHGSGPGQGNSHWRARNRDREYEMGNPPKNFTSSVTGLTAGKYYRFRIVAVYSNNDNKEGNTSLKFFLQNGTSKSNLPVPELREVETLSESAVVLHWSLVSSTQNEDIDGYYAYYRPADSAAEYLKATVDGGMSRSFKIDLLRPGTPYEFKLQSFNSDAASEFSAIRQARTRKTHEQPASSSATPVPANKAVEQHQNSLYPLIAGAAGGGILLLIASLVVCLCLKRRENSQPEDENKPQLEHIQADFVTSAVLGVGGHHKSGDVRRLNGVIPRMNITPNPLAQETASDKNRNVMELRFLPPLANGNGNGNCMARDLALERSNAEEQQDEDGHEDEKGLPAVASSSCETLEACDAAIKLNLPQKHDQEQLDAPNAVLEAPSKPLPPLPKKPANGAGQNNSGLHQNGLHHAQPYHPPGTPTLMHKRLDYHQQPPPVPPHSAYYQQAPTHQPSPMMERSVRGHQQPGGFHMEEGTPTPTRIPSLRRQRRTSGNQQVHNSHSNLNIGLSHHNNNNNNLPPHHQHLHHHPGHPGGLVGIPIVPGSPRVQRSPMPSRAMIKRTRLGSHTDNISSGSLNSIEV

Target ORF information:

RefSeq Version	NM_001103398.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brother of ihog (boi), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103398.4

ORF Insert Sequence:

ATGGCTGAAA TCAGGCTAGT TGTGGTTCTA CTGGTCCTTA TGACCACCTG TATCTATACA 
CTTCAAGCCA GTGACAGTAA TTCCTCCGTG GCAACCACCT TGGGCGTTTT GTTCGAGAGG 
GCTCCCGAAT CAGCGGTGGC CCCGAAAGGA GATGAGGTGG TCTTCGAGTG CGAGCTGAAT 
CTGAAACCTG ATCGCCTGGA GTGGCGGTTT AGACGTGGTG ATTCCTCGGA GCCCTATCTA 
TACCTAAGGC CCTCGGCTGG CTATAACGTG ACCAGCGGAA GCGATGGTCT TGCCTGGAGA 
CTGCGCATCT ATGTGAGTGC CCAAACGGCT GGGGACTACC AGTGTGTGGC TTGGTATGGC 
CCGGGTGCAC TGGCTTCCAC GCCAGCCCGC TTGGCCTTGG TCTCGATTGC TTTGGACGTC 
GGCCAGGGAT CCATGGGGGC CAGGAGCTCC ATCCGCTGGA GCGTGGCGCC CAAAAACTGC 
CTGCTCATTC GTTGCGGCAG CGTAATATCG AATCCGCCGG CTATTTGGAG CTTCTACAGA 
AACGGAAAGA AGCTACCTCA ATCGGAGCTG CTGCCTGGCG CTGCGGGGGC ATTGGTTTTG 
GATACGGTCA CCGCCAAAGA TGCGGGTAAC TACTCGTGCG TGGCCACGAA CTCCATAACA 
GGCGATGAAC TTAGGCTCCC CCAAACTATT GAACTAAGAG TGGACTACAC AGATAGGACA 
CCTCCGTACT TTCTACAACG TCCACCCATC GAATACTCAG CTCGTCCCGG AGAAACAGTG 
GTGCTCGAGT GTCCTGGCGT GGGATCCCCA CGACCCCGAG TCATTTGGAG TAGTCCCAAT 
GTGGTTGAGA TCTATAATAA TAGAAGCACC ATCCTGTCCT ACGGCCTGCA AATCACCGAT 
GTGAAGCCCG AGGATCAGGG TTCCTATATC TGCATGCTGG ACAATGGTAT TGCACCACCG 
ATCGATCACA CCATCAAGTT GAGTGTTTTG CAGCGGCCCA CGATCTTAAG AGGACCGGCC 
GCCACTCTGA CGAATGAGAG CAATCCTTTG CTGCTGGATT GCTCCGCCTC GGGGAATCCC 
CAACCCGATA TTTATTGGCT CATAAACGGT GAGGATGCCA CCAAAGATCC GGAGGCTGTG 
GTGGATAACC GGAGCCTGCA GCTGAAGCGA GTTCAAAAGC GTCACGCTGG CGTGGTTCAG 
TGCTTTGCAA AGAACATTTT GGGAGAGACG AGCGAGGGAA ACATATTGCG CGTGAATCCC 
CTGGAAATCA CCGGCGAGGA TGACGAACCA CTTGGCGAAG TTCCCGTGTG GCCAGTTCAT 
GAATCCAACA CTGGAATGGG CACCTCGTCA ACATCTGGAG GCTCCAAAAA CAAGAGCGGC 
AGACGAAAGT TTAAGGCGAG CATGGTGCCT CCGTCACGAC CCAATGTCAC CCGTCTGTCG 
GACGAGTCCG TGATGCTGCG CTGGAGTGTG CCACAAAACT CTGGACTCCA GATCACCTTT 
TTCAAGGTGC AGTATCGCAT GTTGGGCGAC GGAAAAAATC GGAGGAAGAA CTGGCAGACG 
ACCAGCGACA ATATCCCGTA CGGAAACGCG CACGGATCCG GGCCTGGCCA GGGGAACAGT 
CACTGGCGGG CGCGGAACCG GGACAGGGAG TATGAAATGG GCAATCCGCC CAAGAACTTC 
ACCTCCTCCG TTACGGGACT GACCGCCGGC AAGTACTACC GCTTCCGGAT CGTGGCGGTG 
TACTCCAATA ACGACAACAA GGAGGGCAAC ACCTCCCTCA AGTTCTTCCT GCAGAATGGA 
ACGTCCAAGT CGAATCTGCC AGTGCCGGAA CTACGCGAGG TGGAGACGCT TTCCGAGTCG 
GCCGTGGTGC TCCATTGGTC CCTGGTATCC TCGACTCAAA ATGAGGATAT AGACGGGTAC 
TATGCCTACT ATAGGCCAGC GGACTCAGCA GCGGAGTATT TGAAGGCCAC GGTGGATGGC 
GGAATGAGCA GGAGCTTCAA GATAGACCTC CTTCGTCCAG GAACCCCTTA TGAGTTCAAA 
TTGCAATCGT TCAACTCCGA CGCAGCCTCT GAATTCAGTG CTATTCGGCA AGCGAGGACC 
AGAAAAACGC ACGAGCAACC GGCTTCATCT TCGGCCACGC CAGTGCCAGC CAATAAGGCA 
GTGGAACAGC ACCAGAACTC CTTATATCCA CTGATCGCAG GTGCCGCTGG CGGAGGCATA 
CTACTCCTCA TCGCCTCATT GGTGGTCTGC CTTTGCCTAA AGAGACGGGA AAACTCTCAG 
CCGGAGGATG AGAACAAACC GCAATTGGAA CACATTCAAG CGGACTTTGT TACCTCCGCC 
GTCCTGGGAG TTGGTGGCCA CCACAAAAGT GGCGATGTAA GGCGCCTCAA CGGAGTGATT 
CCGAGGATGA ATATTACGCC CAATCCGCTG GCCCAGGAAA CGGCATCCGA TAAGAACCGC 
AACGTGATGG AGCTGCGTTT CCTGCCGCCG CTGGCGAATG GAAATGGAAA TGGCAACTGC 
ATGGCCAGAG ATCTAGCCCT GGAGCGGTCG AACGCGGAAG AGCAGCAGGA TGAGGACGGA 
CACGAAGATG AAAAGGGGCT ACCGGCGGTT GCCTCGTCCT CCTGCGAAAC GCTGGAGGCC 
TGCGACGCTG CAATTAAACT GAATCTCCCT CAGAAACACG ACCAGGAGCA GCTGGATGCT 
CCGAACGCGG TGCTGGAGGC GCCCAGCAAG CCGCTACCTC CGCTGCCCAA GAAACCAGCA 
AACGGCGCTG GCCAAAACAA CTCTGGACTG CATCAGAATG GCCTTCACCA CGCACAGCCG 
TACCATCCGC CTGGCACGCC CACTCTGATG CACAAGCGAC TGGACTACCA CCAGCAGCCG 
CCGCCGGTGC CTCCGCACTC GGCCTATTAC CAACAGGCAC CCACGCACCA GCCGTCGCCG 
ATGATGGAGC GCAGTGTCAG GGGTCACCAG CAGCCCGGGG GCTTTCATAT GGAGGAGGGC 
ACACCCACGC CGACCAGGAT ACCATCCCTG CGGCGACAGC GACGCACCTC GGGCAACCAG 
CAGGTGCACA ACAGCCACAG CAATCTCAAT ATTGGCCTGA GCCACCACAA CAACAACAAC 
AACAACCTGC CGCCGCACCA CCAGCATCTG CACCACCACC CCGGCCATCC GGGTGGCCTG 
GTGGGCATAC CCATCGTGCC AGGGAGCCCT AGGGTCCAGA GATCGCCGAT GCCGAGCAGG 
GCGATGATCA AGAGGACGCG CCTGGGCAGC CACACGGACA ACATATCGTC GGGCAGTCTG 
AACAGTATTG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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boi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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