Sik2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sik2
Entrez Gene ID	31170
Full Name	Salt-inducible kinase 2
Synonyms	CG4290,Dmel\CG4290,EG:22E5.8,SIK,SIK2
General protein information	Preferred Names Salt-inducible kinase 2 Names CG4290 gene product from transcript CG4290-RA CG4290-PA Salt-induced kinase 2 Sik2-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2C8-2C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_130616.3	NP_569972.1	Salt-inducible kinase 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila salt-inducible kinases Sik2 and Sik3 are negative regulators of Hpo signalling.
Title: Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila.

The following Sik2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sik2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00548	NM_130616.3 Latest version!	Drosophila melanogaster Salt-inducible kinase 2 (Sik2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sik2 ( NM_130616.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569972.1 Drosophila melanogaster Salt-inducible kinase 2 (Sik2), mRNA.

Price & Availability↑

CloneID	OFa00548
Clone ID Related Accession (Same CDS sequence)	NM_130616.3
Accession Version	NM_130616.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Salt-inducible kinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_130616.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCACGT GCGAGGCGGC GGCCGCTGGC GAGAATGGGA GCCAGGCCAA GTCCGAGGAG 
AGCCAGCCGC CGGAGGATCA GAAGAAGAAG CAGCCGCAGC ATGAGAGGAA CGAGAAGCAG 
CTGGATAAGC CGCCGGAGAA TTTGCCCCAA AACGGGAAAA CCGAAGCAAA AGGAGCAGAA 
GGAGCGTGTT CACACCCCCC ACTGGATGCC CTCCGGAGCT CAGTGCTCCT GGATGCCGGT 
GCTGCTTCGC CCTCGATAGA CGCAATAGTG GCTTGCAAGG ATGCACTGCT CGCCCAGAAG 
CTCTTTGCCA GCGGGGGTGG CTCCACGCCC GGTCCCAGTC CCACATCCTC TGCGGTGGGC 
GCGGGCGGTA TCAGTGGCAA GGACCTGCTC AAACTCAAGG AGCCCATGCG CGTGGGCTTC 
TACGACATCG AGCGCACCAT TGGCAAGGGA AATTTTGCAG TGGTCAAGCT GGCCCGGCAT 
CGAATCACCA AGAACGAGGT GGCTATCAAG ATCATTGACA AGTCGCAGCT GGACCAGACA 
AACCTACAGA AAGTCTACCG CGAAGTGGAG ATCATGAAGC GCCTGAAGCA CCCTCACATC 
ATTAAGCTCT ACCAGGTTAT GGAGACCAAG AATATGATCT ACATAGTGTC GGAGTATGCC 
AGCCAAGGAG AGATCTTCGA TTACATTGCC AAGTACGGGC GGATGTCCGA GTCGGCGGCG 
CGCTTTAAGT TCTGGCAGAT CATATCGGCG GTGGAGTACT GTCACAAGAA GGGCATAGTG 
CATCGGGATC TCAAGGCGGA GAACCTGCTC CTGGATTTGA ACATGAACAT CAAAATAGCG 
GATTTCGGGT TCTCCAATCA CTTCAAGCCG GGAGAATTAC TGGCCACCTG GTGCGGATCA 
CCGCCGTACG CAGCTCCCGA GGTTTTCGAG GGAAAGCAAT ATACGGGTCC AGAGATTGAT 
ATTTGGTCAC TGGGCGTCGT GCTTTATGTG CTGGTATGTG GGGCCCTGCC CTTCGACGGA 
TCCACGCTGC AGAGCCTCCG GGATCGGGTG CTTTCCGGTC GCTTTCGCAT TCCGTTTTTT 
ATGAGCTCCG AGTGCGAGCA CCTCATTCGC CGGATGCTGG TACTGGAGCC CACAAGGCGG 
TACACCATCG ACCAAATCAA ACGCCATCGC TGGATGTGCC CCGAGCTGCT GGAGCACGTC 
CTGATAGCCA AGTACAATCT GGGCGCCGAG CGGCAGACGT CCGTGGAACC CAGCGAGGAC 
ATTCTTCGCA TCATGGCCGA GTATGTCGGC ATTGGGTCGG ACAAGACGCG AGCCAGTCTT 
AAGAAGAACA CTTACGACCA TGTCGCGGCC ATATATCTGC TCCTGCAGGA TCGAGTTAGC 
CACAAGAAGG AGCAGAGCAA CGGCTTAGGC GCCAGCGCCC TTGCCTCGTC GACCTCCGCC 
AGTCGGATGA TCTACAGCTC CAGAAATGAC CACCAACCGA CGCAGCAACA ATCGCAGCAG 
CAGTCCAAGA CAATATCCAC CAGCTCGATC CTGGCCAAGG ATCAGTGCCA CAAAAGACTT 
AGCCGCCATC AGACGGTACT CATGAGTGAG CGTAACGCCC ACGCCGGCGC GACACCCACA 
GTTCCAGATC CCGGTCCCGG ATACTACGCC AAGTATGGCC CACTGCAACT GCCACTGCCT 
CTGACGGGCC ACTCCCACCT GACTGGGTAT CTGAATGGAG GAGGCGTCGA GGTGGATGCC 
AGCGGCATTC CGCTGCCCAT GCGGTACACC CCGCTGCCGA CAGCCGCGTC GCCCGCTCCC 
TCTAACTGCT CATCCACATC CTCGAGGGTA GGACGACACA GTCTAAGCTC CAGCTCGCCG 
CGAAGCCATC GACCGGTGGC CATTTCGCTC AGCATCGACA ACAACCCCTC GCTGGCCAAC 
CTGCGGTGCA GGGAGATGAT GGAGGCTGGA GGAGGACCCG TTGGAGCAGT GGGTGTGCCT 
CTAGCGTCCA AGCAGCTTCA TCAGACCATC AGCGAGTTCA TCATCAAACA GAGCACCGAA 
GACTGTCGTG CGCTGCTGCA GCAGTCCACA GCAGTGGCTG AGGGTAAAGA TGATCCGCCA 
AAGGCGGAAT CAAGCGTAGG AGGCGTTCCT CCGCCCGCCT CTACCACACC CACATCCAGT 
ACAGCGGGAC CGGAATCGGG ATCGGCTCCC TGCCCTGGTG AGATTAACGG CAAGACAATA 
AAGACTATGT CCAGCTCGAG CAGCTTCGAT TCTAAGGCCA ATTTGGGCCA AAGCTTTCGC 
TACAAGATGT CCGCCGAGGC CAGCAAGCTT TTCCAGACTC TCCAGGAAAG CCCACTGCCC 
GTGGAGCAAA GGACTAAGCG TCGTGTGCAC GTGGGCAGCA CCAATGGCAG CGGCGGTGAC 
TCCGGCCAGG AAACGAATGA TGCGAAATCG AATGGCGACA GCCGCTCGGA GAAGAAAGTT 
CTGGCCCAAG GCAGCTCCAG CACAGATGAG GGCTGCGAGA CAGACCAGGG CAACGATCCG 
GGCTCTGCTT CCCAGGAGTC AAAGGGCAGC AATGGCGGCG GTAGCGGTAA CGCCAACGGC 
GGACCCACCT CGCACTCCAG CAGCGATCTG ACGCGGCTCG TTGGAACCAC TACCTCTGGA 
CAGAGTCACA AGATGCGCTC GTATGCCAGT AGTAGCTCGT CCAGTGGCGT TCTCGCCGGG 
AGCGCTGGTA GCTACTCCAA AAGTCTGAGC CAGAACCTGA GCCGCGGCTC GTCCAAGAGC 
AATTGCAGCG GTCCCTACGA TAGCTTGGAC TTTGCCTTGC CTTCTGGCAA GGGCAGTCTA 
CCCTCGTGCA TGGGCTCAAG TTCGATGCTG GCCACGCCCA CTCCGGCCTC GGCATCGCCA 
GCGGGAATCT CATCGGAGCA TTCATCGGAA AGATCGCTGT ACGGGAGCCA CAACTCCTGC 
ATCCATATGC CAGGAGCTCT GCCGTTGGGC TTGGGCCTTC CTCAGAGTTC AGCCTCCACG 
CCCACGCCGA ATCCTACGCC TCCGCCAAAT GGCGGTGGTG TCACGTTCCT GGACAAGCGT 
TCACCCATAC ACTTTCGCGA AGGAAGACGG GCGAGTGATG GCCTGGTGGC CCAGGGCCTG 
CTTAGCTCTG GTTCCTTGCT GGGCACCAGT CGTGTGTACG GAAGTTATCG CTACGAGCAG 
GCCAAGCGTC ACGGTTGGCT GGAGATCCAG CAGCTGCAGC AGCTGCAGCA GGAGGCGGCT 
GTGGGGCATT CACACCCACA TGCCCATCAA CATCCGCATC AGCATCCGCA TCCTCATCCC 
CAAGCGTACG GCTTGGAAGA ACTCTGTCAA TTTCCAAATG GTCAATTCTA TGCTCTGCCG 
GGCAAGCATC ATCCGCTGCT GACTTTGCCC CATCATCATG CGCATCCTGC GCAGCATCAC 
CATGGCCATC ATTCGCTGTT CCATTCAGGC CACCAAGCCA CACCTCTGCT CCTGGAAGCC 
GCTGCTGGTG GGGATATGTA TGGTCATGGA TGCTATGCTC CGCCACCGCC GCCACCAGGG 
CTATACACGC ACCATCAGTT GGGCGTGGGT ATGGCCGTGC CCATGTCCCC GATGCAAAAG 
CCACCGCTGC AGCAGCAGCT ACTGCAGCAC CGCCTGCTTC AGCAGAAGCG TCAGTTGTTC 
CAGAAGCAGT ACGCACTGGA GGCGCAGCTA GCCGGTCGAC ATCACCACCA TCAGCAGCAC 
AGCCATCACC ACCATCACCA CTTTGGCCAC AGCCTGGGTC CGCCACCGCC GCCGGCTCCG 
GCTCCGGAAC ATCACCTGGC CGACGAGTTG TACGAGTTGG CCTTGCTTGA TCGGCCCAGG 
AGCGGAACTC CTAGGCTTCA GCACTCCATG ACCATGCCCG GAGCTCATGC CTTCAGTCAG 
ATGAACCTCA AGACTTCCTA CATCAGCCAA TCGGACCAGG TGCCTGGTGC GGCGCCTAAT 
GGAGCTGGTT CCGGAGGATC AGCTGCTGGG GTATCATGCT CAGCTCCACC GTCCACGGCG 
CCGGGTACGC CCACGGTCAA GTGCAAGCCT ACCACGCCCC ACGGTCACAG CCTGGACTCC 
GACTATCATA CATCGACGCC GGTGCTCAGC CTTTTCACGC CCAATTGGCA GTCGCTGGTA 
AAGCCGCTCA GCGAGAGCCC CATCCTGGAG ATATCGGAGC ACCTTGAGTC AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569972.1
CDS	426..4622
Translation	MSTCEAAAAGENGSQAKSEESQPPEDQKKKQPQHERNEKQLDKPPENLPQNGKTEAKGAEGACSHPPLDALRSSVLLDAGAASPSIDAIVACKDALLAQKLFASGGGSTPGPSPTSSAVGAGGISGKDLLKLKEPMRVGFYDIERTIGKGNFAVVKLARHRITKNEVAIKIIDKSQLDQTNLQKVYREVEIMKRLKHPHIIKLYQVMETKNMIYIVSEYASQGEIFDYIAKYGRMSESAARFKFWQIISAVEYCHKKGIVHRDLKAENLLLDLNMNIKIADFGFSNHFKPGELLATWCGSPPYAAPEVFEGKQYTGPEIDIWSLGVVLYVLVCGALPFDGSTLQSLRDRVLSGRFRIPFFMSSECEHLIRRMLVLEPTRRYTIDQIKRHRWMCPELLEHVLIAKYNLGAERQTSVEPSEDILRIMAEYVGIGSDKTRASLKKNTYDHVAAIYLLLQDRVSHKKEQSNGLGASALASSTSASRMIYSSRNDHQPTQQQSQQQSKTISTSSILAKDQCHKRLSRHQTVLMSERNAHAGATPTVPDPGPGYYAKYGPLQLPLPLTGHSHLTGYLNGGGVEVDASGIPLPMRYTPLPTAASPAPSNCSSTSSRVGRHSLSSSSPRSHRPVAISLSIDNNPSLANLRCREMMEAGGGPVGAVGVPLASKQLHQTISEFIIKQSTEDCRALLQQSTAVAEGKDDPPKAESSVGGVPPPASTTPTSSTAGPESGSAPCPGEINGKTIKTMSSSSSFDSKANLGQSFRYKMSAEASKLFQTLQESPLPVEQRTKRRVHVGSTNGSGGDSGQETNDAKSNGDSRSEKKVLAQGSSSTDEGCETDQGNDPGSASQESKGSNGGGSGNANGGPTSHSSSDLTRLVGTTTSGQSHKMRSYASSSSSSGVLAGSAGSYSKSLSQNLSRGSSKSNCSGPYDSLDFALPSGKGSLPSCMGSSSMLATPTPASASPAGISSEHSSERSLYGSHNSCIHMPGALPLGLGLPQSSASTPTPNPTPPPNGGGVTFLDKRSPIHFREGRRASDGLVAQGLLSSGSLLGTSRVYGSYRYEQAKRHGWLEIQQLQQLQQEAAVGHSHPHAHQHPHQHPHPHPQAYGLEELCQFPNGQFYALPGKHHPLLTLPHHHAHPAQHHHGHHSLFHSGHQATPLLLEAAAGGDMYGHGCYAPPPPPPGLYTHHQLGVGMAVPMSPMQKPPLQQQLLQHRLLQQKRQLFQKQYALEAQLAGRHHHHQQHSHHHHHHFGHSLGPPPPPAPAPEHHLADELYELALLDRPRSGTPRLQHSMTMPGAHAFSQMNLKTSYISQSDQVPGAAPNGAGSGGSAAGVSCSAPPSTAPGTPTVKCKPTTPHGHSLDSDYHTSTPVLSLFTPNWQSLVKPLSESPILEISEHLESV

Target ORF information:

RefSeq Version	NM_130616.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Salt-inducible kinase 2 (Sik2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130616.3

ORF Insert Sequence:

ATGAGCACGT GCGAGGCGGC GGCCGCTGGC GAGAATGGGA GCCAGGCCAA GTCCGAGGAG 
AGCCAGCCGC CGGAGGATCA GAAGAAGAAG CAGCCGCAGC ATGAGAGGAA CGAGAAGCAG 
CTGGATAAGC CGCCGGAGAA TTTGCCCCAA AACGGGAAAA CCGAAGCAAA AGGAGCAGAA 
GGAGCGTGTT CACACCCCCC ACTGGATGCC CTCCGGAGCT CAGTGCTCCT GGATGCCGGT 
GCTGCTTCGC CCTCGATAGA CGCAATAGTG GCTTGCAAGG ATGCACTGCT CGCCCAGAAG 
CTCTTTGCCA GCGGGGGTGG CTCCACGCCC GGTCCCAGTC CCACATCCTC TGCGGTGGGC 
GCGGGCGGTA TCAGTGGCAA GGACCTGCTC AAACTCAAGG AGCCCATGCG CGTGGGCTTC 
TACGACATCG AGCGCACCAT TGGCAAGGGA AATTTTGCAG TGGTCAAGCT GGCCCGGCAT 
CGAATCACCA AGAACGAGGT GGCTATCAAG ATCATTGACA AGTCGCAGCT GGACCAGACA 
AACCTACAGA AAGTCTACCG CGAAGTGGAG ATCATGAAGC GCCTGAAGCA CCCTCACATC 
ATTAAGCTCT ACCAGGTTAT GGAGACCAAG AATATGATCT ACATAGTGTC GGAGTATGCC 
AGCCAAGGAG AGATCTTCGA TTACATTGCC AAGTACGGGC GGATGTCCGA GTCGGCGGCG 
CGCTTTAAGT TCTGGCAGAT CATATCGGCG GTGGAGTACT GTCACAAGAA GGGCATAGTG 
CATCGGGATC TCAAGGCGGA GAACCTGCTC CTGGATTTGA ACATGAACAT CAAAATAGCG 
GATTTCGGGT TCTCCAATCA CTTCAAGCCG GGAGAATTAC TGGCCACCTG GTGCGGATCA 
CCGCCGTACG CAGCTCCCGA GGTTTTCGAG GGAAAGCAAT ATACGGGTCC AGAGATTGAT 
ATTTGGTCAC TGGGCGTCGT GCTTTATGTG CTGGTATGTG GGGCCCTGCC CTTCGACGGA 
TCCACGCTGC AGAGCCTCCG GGATCGGGTG CTTTCCGGTC GCTTTCGCAT TCCGTTTTTT 
ATGAGCTCCG AGTGCGAGCA CCTCATTCGC CGGATGCTGG TACTGGAGCC CACAAGGCGG 
TACACCATCG ACCAAATCAA ACGCCATCGC TGGATGTGCC CCGAGCTGCT GGAGCACGTC 
CTGATAGCCA AGTACAATCT GGGCGCCGAG CGGCAGACGT CCGTGGAACC CAGCGAGGAC 
ATTCTTCGCA TCATGGCCGA GTATGTCGGC ATTGGGTCGG ACAAGACGCG AGCCAGTCTT 
AAGAAGAACA CTTACGACCA TGTCGCGGCC ATATATCTGC TCCTGCAGGA TCGAGTTAGC 
CACAAGAAGG AGCAGAGCAA CGGCTTAGGC GCCAGCGCCC TTGCCTCGTC GACCTCCGCC 
AGTCGGATGA TCTACAGCTC CAGAAATGAC CACCAACCGA CGCAGCAACA ATCGCAGCAG 
CAGTCCAAGA CAATATCCAC CAGCTCGATC CTGGCCAAGG ATCAGTGCCA CAAAAGACTT 
AGCCGCCATC AGACGGTACT CATGAGTGAG CGTAACGCCC ACGCCGGCGC GACACCCACA 
GTTCCAGATC CCGGTCCCGG ATACTACGCC AAGTATGGCC CACTGCAACT GCCACTGCCT 
CTGACGGGCC ACTCCCACCT GACTGGGTAT CTGAATGGAG GAGGCGTCGA GGTGGATGCC 
AGCGGCATTC CGCTGCCCAT GCGGTACACC CCGCTGCCGA CAGCCGCGTC GCCCGCTCCC 
TCTAACTGCT CATCCACATC CTCGAGGGTA GGACGACACA GTCTAAGCTC CAGCTCGCCG 
CGAAGCCATC GACCGGTGGC CATTTCGCTC AGCATCGACA ACAACCCCTC GCTGGCCAAC 
CTGCGGTGCA GGGAGATGAT GGAGGCTGGA GGAGGACCCG TTGGAGCAGT GGGTGTGCCT 
CTAGCGTCCA AGCAGCTTCA TCAGACCATC AGCGAGTTCA TCATCAAACA GAGCACCGAA 
GACTGTCGTG CGCTGCTGCA GCAGTCCACA GCAGTGGCTG AGGGTAAAGA TGATCCGCCA 
AAGGCGGAAT CAAGCGTAGG AGGCGTTCCT CCGCCCGCCT CTACCACACC CACATCCAGT 
ACAGCGGGAC CGGAATCGGG ATCGGCTCCC TGCCCTGGTG AGATTAACGG CAAGACAATA 
AAGACTATGT CCAGCTCGAG CAGCTTCGAT TCTAAGGCCA ATTTGGGCCA AAGCTTTCGC 
TACAAGATGT CCGCCGAGGC CAGCAAGCTT TTCCAGACTC TCCAGGAAAG CCCACTGCCC 
GTGGAGCAAA GGACTAAGCG TCGTGTGCAC GTGGGCAGCA CCAATGGCAG CGGCGGTGAC 
TCCGGCCAGG AAACGAATGA TGCGAAATCG AATGGCGACA GCCGCTCGGA GAAGAAAGTT 
CTGGCCCAAG GCAGCTCCAG CACAGATGAG GGCTGCGAGA CAGACCAGGG CAACGATCCG 
GGCTCTGCTT CCCAGGAGTC AAAGGGCAGC AATGGCGGCG GTAGCGGTAA CGCCAACGGC 
GGACCCACCT CGCACTCCAG CAGCGATCTG ACGCGGCTCG TTGGAACCAC TACCTCTGGA 
CAGAGTCACA AGATGCGCTC GTATGCCAGT AGTAGCTCGT CCAGTGGCGT TCTCGCCGGG 
AGCGCTGGTA GCTACTCCAA AAGTCTGAGC CAGAACCTGA GCCGCGGCTC GTCCAAGAGC 
AATTGCAGCG GTCCCTACGA TAGCTTGGAC TTTGCCTTGC CTTCTGGCAA GGGCAGTCTA 
CCCTCGTGCA TGGGCTCAAG TTCGATGCTG GCCACGCCCA CTCCGGCCTC GGCATCGCCA 
GCGGGAATCT CATCGGAGCA TTCATCGGAA AGATCGCTGT ACGGGAGCCA CAACTCCTGC 
ATCCATATGC CAGGAGCTCT GCCGTTGGGC TTGGGCCTTC CTCAGAGTTC AGCCTCCACG 
CCCACGCCGA ATCCTACGCC TCCGCCAAAT GGCGGTGGTG TCACGTTCCT GGACAAGCGT 
TCACCCATAC ACTTTCGCGA AGGAAGACGG GCGAGTGATG GCCTGGTGGC CCAGGGCCTG 
CTTAGCTCTG GTTCCTTGCT GGGCACCAGT CGTGTGTACG GAAGTTATCG CTACGAGCAG 
GCCAAGCGTC ACGGTTGGCT GGAGATCCAG CAGCTGCAGC AGCTGCAGCA GGAGGCGGCT 
GTGGGGCATT CACACCCACA TGCCCATCAA CATCCGCATC AGCATCCGCA TCCTCATCCC 
CAAGCGTACG GCTTGGAAGA ACTCTGTCAA TTTCCAAATG GTCAATTCTA TGCTCTGCCG 
GGCAAGCATC ATCCGCTGCT GACTTTGCCC CATCATCATG CGCATCCTGC GCAGCATCAC 
CATGGCCATC ATTCGCTGTT CCATTCAGGC CACCAAGCCA CACCTCTGCT CCTGGAAGCC 
GCTGCTGGTG GGGATATGTA TGGTCATGGA TGCTATGCTC CGCCACCGCC GCCACCAGGG 
CTATACACGC ACCATCAGTT GGGCGTGGGT ATGGCCGTGC CCATGTCCCC GATGCAAAAG 
CCACCGCTGC AGCAGCAGCT ACTGCAGCAC CGCCTGCTTC AGCAGAAGCG TCAGTTGTTC 
CAGAAGCAGT ACGCACTGGA GGCGCAGCTA GCCGGTCGAC ATCACCACCA TCAGCAGCAC 
AGCCATCACC ACCATCACCA CTTTGGCCAC AGCCTGGGTC CGCCACCGCC GCCGGCTCCG 
GCTCCGGAAC ATCACCTGGC CGACGAGTTG TACGAGTTGG CCTTGCTTGA TCGGCCCAGG 
AGCGGAACTC CTAGGCTTCA GCACTCCATG ACCATGCCCG GAGCTCATGC CTTCAGTCAG 
ATGAACCTCA AGACTTCCTA CATCAGCCAA TCGGACCAGG TGCCTGGTGC GGCGCCTAAT 
GGAGCTGGTT CCGGAGGATC AGCTGCTGGG GTATCATGCT CAGCTCCACC GTCCACGGCG 
CCGGGTACGC CCACGGTCAA GTGCAAGCCT ACCACGCCCC ACGGTCACAG CCTGGACTCC 
GACTATCATA CATCGACGCC GGTGCTCAGC CTTTTCACGC CCAATTGGCA GTCGCTGGTA 
AAGCCGCTCA GCGAGAGCCC CATCCTGGAG ATATCGGAGC ACCTTGAGTC AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sik2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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