Edem1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Edem1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Edem1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa00531 NM_166915.2
Latest version!
Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa00531 NM_001258554.2
Latest version!
Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa00531 NM_166914.2
Latest version!
Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa00531 NM_130603.3
Latest version!
Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa03766 NM_001272223.2
Latest version!
Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa00531
    Clone ID Related Accession (Same CDS sequence) NM_166915.2 , NM_130603.3 , NM_001258554.2 , NM_166914.2
    Accession Version NM_166915.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product ER degradation enhancer, mannosidase alpha-like 1, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_166915.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
    GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA
    CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC
    TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC
    TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC
    CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC
    GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG
    CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG
    GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC
    ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC
    GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC
    GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT
    TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA
    GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA
    GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG
    GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC
    CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC
    ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC
    ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC
    GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG
    CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC
    AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC
    AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC
    GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC
    ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC
    ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA
    AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG
    TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC
    GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA
    CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA
    AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT
    GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA
    AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC
    GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG
    AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT
    CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA
    GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC
    GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA
    AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT
    ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA
    GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC
    CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT
    CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC
    TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT
    GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG
    CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG
    GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC
    CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG
    TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA
    GCGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_726778.1
    CDS677..3625
    Translation

    Target ORF information:

    RefSeq Version NM_166915.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_166915.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
    GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA
    CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC
    TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC
    TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC
    CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC
    GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG
    CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG
    GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC
    ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC
    GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC
    GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT
    TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA
    GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA
    GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG
    GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC
    CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC
    ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC
    ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC
    GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG
    CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC
    AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC
    AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC
    GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC
    ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC
    ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA
    AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG
    TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC
    GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA
    CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA
    AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT
    GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA
    AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC
    GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG
    AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT
    CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA
    GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC
    GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA
    AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT
    ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA
    GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC
    CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT
    CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC
    TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT
    GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG
    CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG
    GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC
    CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG
    TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA
    GCGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00531
    Clone ID Related Accession (Same CDS sequence) NM_166915.2 , NM_130603.3 , NM_001258554.2 , NM_166914.2
    Accession Version NM_130603.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product ER degradation enhancer, mannosidase alpha-like 1, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_130603.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
    GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA
    CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC
    TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC
    TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC
    CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC
    GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG
    CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG
    GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC
    ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC
    GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC
    GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT
    TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA
    GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA
    GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG
    GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC
    CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC
    ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC
    ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC
    GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG
    CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC
    AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC
    AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC
    GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC
    ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC
    ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA
    AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG
    TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC
    GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA
    CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA
    AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT
    GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA
    AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC
    GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG
    AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT
    CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA
    GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC
    GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA
    AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT
    ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA
    GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC
    CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT
    CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC
    TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT
    GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG
    CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG
    GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC
    CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG
    TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA
    GCGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_569959.1
    CDS54..3002
    Translation

    Target ORF information:

    RefSeq Version NM_130603.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_130603.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
    GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA
    CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC
    TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC
    TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC
    CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC
    GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG
    CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG
    GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC
    ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC
    GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC
    GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT
    TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA
    GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA
    GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG
    GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC
    CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC
    ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC
    ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC
    GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG
    CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC
    AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC
    AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC
    GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC
    ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC
    ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA
    AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG
    TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC
    GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA
    CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA
    AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT
    GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA
    AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC
    GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG
    AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT
    CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA
    GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC
    GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA
    AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT
    ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA
    GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC
    CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT
    CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC
    TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT
    GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG
    CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG
    GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC
    CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG
    TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA
    GCGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00531
    Clone ID Related Accession (Same CDS sequence) NM_166915.2 , NM_130603.3 , NM_001258554.2 , NM_166914.2
    Accession Version NM_001258554.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product ER degradation enhancer, mannosidase alpha-like 1, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001258554.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
    GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA
    CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC
    TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC
    TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC
    CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC
    GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG
    CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG
    GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC
    ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC
    GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC
    GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT
    TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA
    GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA
    GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG
    GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC
    CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC
    ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC
    ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC
    GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG
    CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC
    AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC
    AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC
    GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC
    ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC
    ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA
    AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG
    TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC
    GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA
    CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA
    AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT
    GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA
    AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC
    GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG
    AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT
    CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA
    GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC
    GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA
    AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT
    ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA
    GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC
    CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT
    CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC
    TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT
    GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG
    CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG
    GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC
    CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG
    TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA
    GCGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245483.1
    CDS361..3309
    Translation

    Target ORF information:

    RefSeq Version NM_001258554.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258554.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
    GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA
    CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC
    TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC
    TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC
    CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC
    GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG
    CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG
    GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC
    ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC
    GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC
    GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT
    TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA
    GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA
    GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG
    GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC
    CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC
    ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC
    ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC
    GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG
    CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC
    AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC
    AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC
    GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC
    ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC
    ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA
    AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG
    TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC
    GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA
    CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA
    AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT
    GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA
    AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC
    GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG
    AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT
    CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA
    GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC
    GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA
    AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT
    ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA
    GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC
    CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT
    CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC
    TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT
    GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG
    CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG
    GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC
    CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG
    TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA
    GCGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00531
    Clone ID Related Accession (Same CDS sequence) NM_166915.2 , NM_130603.3 , NM_001258554.2 , NM_166914.2
    Accession Version NM_166914.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product ER degradation enhancer, mannosidase alpha-like 1, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_166914.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
    GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA
    CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC
    TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC
    TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC
    CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC
    GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG
    CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG
    GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC
    ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC
    GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC
    GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT
    TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA
    GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA
    GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG
    GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC
    CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC
    ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC
    ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC
    GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG
    CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC
    AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC
    AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC
    GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC
    ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC
    ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA
    AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG
    TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC
    GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA
    CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA
    AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT
    GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA
    AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC
    GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG
    AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT
    CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA
    GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC
    GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA
    AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT
    ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA
    GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC
    CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT
    CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC
    TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT
    GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG
    CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG
    GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC
    CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG
    TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA
    GCGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_726777.1
    CDS503..3451
    Translation

    Target ORF information:

    RefSeq Version NM_166914.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_166914.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
    GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA
    CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC
    TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC
    TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC
    CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC
    GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG
    CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG
    GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC
    ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC
    GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC
    GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT
    TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA
    GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA
    GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG
    GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC
    CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC
    ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC
    ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC
    GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG
    CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC
    AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC
    AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC
    GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC
    ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC
    ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA
    AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG
    TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC
    GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA
    CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA
    AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT
    GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA
    AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC
    GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG
    AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT
    CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA
    GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC
    GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA
    AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT
    ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA
    GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC
    CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT
    CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC
    TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT
    GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG
    CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG
    GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC
    CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG
    TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA
    GCGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03766
    Clone ID Related Accession (Same CDS sequence) NM_001272223.2
    Accession Version NM_001272223.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product ER degradation enhancer, mannosidase alpha-like 1, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272223.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
    GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA
    CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC
    TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC
    TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC
    CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC
    GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG
    CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG
    GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC
    ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC
    GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC
    GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT
    TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA
    GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA
    GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG
    GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC
    CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC
    ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC
    ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC
    GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG
    CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC
    AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC
    AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC
    GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC
    ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC
    ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA
    AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG
    TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC
    GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA
    CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA
    AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT
    GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA
    AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC
    GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG
    AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT
    CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA
    GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC
    GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA
    AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT
    ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA
    GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC
    CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT
    CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC
    TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT
    GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG
    CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG
    GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC
    CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG
    TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA
    GCGCTCAAAG TTCGGCGTCG ATGCGCGCAT GACAATGAGC GACTCAATTG CAGAGCGAAA
    AGGACAGAGC GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259152.1
    CDS361..3375
    Translation

    Target ORF information:

    RefSeq Version NM_001272223.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272223.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
    GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA
    CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC
    TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC
    TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC
    CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC
    GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG
    CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG
    GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC
    ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC
    GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC
    GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT
    TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA
    GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA
    GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG
    GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC
    CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC
    ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC
    ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC
    GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG
    CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC
    AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC
    AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC
    GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC
    ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC
    ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA
    AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG
    TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC
    GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA
    CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA
    AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT
    GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA
    AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC
    GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG
    AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT
    CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA
    GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC
    GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA
    AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT
    ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA
    GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC
    CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT
    CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC
    TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT
    GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG
    CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG
    GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC
    CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG
    TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA
    GCGCTCAAAG TTCGGCGTCG ATGCGCGCAT GACAATGAGC GACTCAATTG CAGAGCGAAA
    AGGACAGAGC GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.