Edem1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Edem1
Entrez Gene ID	31154
Full Name	ER degradation enhancer, mannosidase alpha-like 1
Synonyms	CG3810,DM-pI,Dmel\CG3810,EDEM1,EG.86E4.2,EG:86E4.2,edem1
General protein information	Preferred Names ER degradation enhancer, mannosidase alpha-like 1 Names CG3810 gene product from transcript CG3810-RC CG3810-PA CG3810-PB CG3810-PC CG3810-PD CG3810-PE Edem1-PA Edem1-PB Edem1-PC Edem1-PD Edem1-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2B15-2B15

mRNA and Protein(s)

mRNA	Protein	Name
NM_166915.2	NP_726778.1	ER degradation enhancer, mannosidase alpha-like 1, isoform C
NM_130603.3	NP_569959.1	ER degradation enhancer, mannosidase alpha-like 1, isoform B
NM_001258554.2	NP_001245483.1	ER degradation enhancer, mannosidase alpha-like 1, isoform D
NM_166914.2	NP_726777.1	ER degradation enhancer, mannosidase alpha-like 1, isoform A
NM_001272223.2	NP_001259152.1	ER degradation enhancer, mannosidase alpha-like 1, isoform E

Pathways from BioSystems

Homologies

Homo sapiens (human)	EDEM2	NP_060687.2
	EDEM2	XP_001101476.1
Danio rerio (zebrafish)	edem2	XP_005174174.1
Bos taurus (cattle)	EDEM2	NP_001094555.1
Mus musculus (house mouse)	Edem2	NP_663512.2
Drosophila melanogaster (fruit fly)	Edem1	NP_001245483.1
Xenopus tropicalis (tropical clawed frog)	edem2	NP_001005031.1
Pan troglodytes (chimpanzee)	EDEM2	XP_514601.1
Canis lupus familiaris (dog)	EDEM2	XP_534400.1
Rattus norvegicus (Norway rat)	Edem2	NP_001004230.1
Oryza sativa (rice)	Os01g0773600	NP_001044401.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012463	XP_001231048.2
Caenorhabditis elegans (roundworm)	F10C2.5	NP_506018.1
Arabidopsis thaliana (thale cress)	AT5G43710	NP_199184.1
Gallus gallus (chicken)	EDEM2	XP_003642546.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Edem1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Edem1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00531	NM_166915.2 Latest version!	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00531	NM_001258554.2 Latest version!	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00531	NM_166914.2 Latest version!	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00531	NM_130603.3 Latest version!	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03766	NM_001272223.2 Latest version!	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Edem1 ( NM_166915.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726778.1 Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00531
Clone ID Related Accession (Same CDS sequence)	NM_166915.2 , NM_130603.3 , NM_001258554.2 , NM_166914.2
Accession Version	NM_166915.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ER degradation enhancer, mannosidase alpha-like 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_166915.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA 
CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC 
TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC 
TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC 
CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC 
GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG 
CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG 
GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC 
ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC 
GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC 
GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT 
TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA 
GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA 
GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG 
GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC 
CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC 
ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC 
ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC 
GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG 
CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC 
AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC 
AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC 
GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC 
ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC 
ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA 
AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG 
TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC 
GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA 
CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA 
AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT 
GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA 
AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC 
GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG 
AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT 
CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA 
GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC 
GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA 
AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT 
ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA 
GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC 
CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT 
CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC 
TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT 
GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG 
CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG 
GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC 
CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG 
TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA 
GCGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726778.1
CDS	677..3625
Translation	MQQKPRNNGYPGTLICAVLLVLLSTGVSGQKQYSKARKLELREDVRRMFQHAYDGYLRHASNYDELRPLTCDGHDTWGSYSLTLIDALDTLATMGNFTEFRRAARLLEEKMDFDRDINVSVFETNIRIVGGLLSAHLLSKRAGVELEPGWPCKGPLLRLAEDVARRLLPAFVTNTGMPYGTVNLRYGVPKGETSITCTAGVGTFLIEFGTLSRLTGKTIYEDVAMKAVHALWAYRSPIGLFGNHIDVQSGRWTALDSGIGAGVDSLFEYLVKASVLLNRPELLELFHEARAAIDKYMRKEDWYVWVGMNKGRVTLPVFQSLEAFWPGILSIIGDTEPALRTISRYIGVWKKYGFLPEFYNIAAGEASPNREVYPLRPELIESAMYLYRATGNEYLLEFGEHMLETLEFSAKTKCGYATIRNVVTHEKENRMESFFLAETSKYLYLLFDEENFLHNDGSGGELLSTEDDVCVVQAGAYIFNTEAHPMDMSALHCCHAHNEDIYTSLDLQRFSPRAILERSKKRQVAVQEQWVPQCHPENNEFFNKEDESVQAQDSEKEQEREQSGTSTTMAVDIEVFDEFQQPAGDLLVSNFEQIREERELNESLHRNVVARNQLTVSDLDEFFAQRRESFASASEALNYVKTFMGNYTMDVAFIRGLQLYDTNMSSVLGTGAQKEYESRMRTLWQLYELQQQYVANIRVIQGLGLLTFPADGDRIPSHLTEVLDSLDKSAHLEHMDLQDLETRVSNTSSPQLAHIREVILKARNAYAIAMVNTSAMQEFAIRIYLSGTSEAGVRIHPLLEDGELSEEARKRPLKLAEERALFSYARRIVDFRKRMAETVDRLQTLMQDIPPPKRKEATTDSQAADASTVYTQTKAQMAAPAQQPEPSQEEKQSTQIQKGKLAENGSGLGEEESGCVWSQLVQTILRKTTVQRVKFDEAVLQENTRKALEKYARKELPHHLFACHRPEYIEGFAYRDFYPEAL

Target ORF information:

RefSeq Version	NM_166915.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166915.2

ORF Insert Sequence:

ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA 
CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC 
TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC 
TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC 
CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC 
GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG 
CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG 
GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC 
ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC 
GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC 
GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT 
TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA 
GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA 
GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG 
GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC 
CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC 
ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC 
ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC 
GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG 
CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC 
AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC 
AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC 
GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC 
ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC 
ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA 
AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG 
TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC 
GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA 
CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA 
AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT 
GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA 
AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC 
GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG 
AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT 
CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA 
GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC 
GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA 
AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT 
ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA 
GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC 
CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT 
CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC 
TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT 
GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG 
CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG 
GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC 
CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG 
TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA 
GCGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Edem1 ( NM_130603.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569959.1 Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00531
Clone ID Related Accession (Same CDS sequence)	NM_166915.2 , NM_130603.3 , NM_001258554.2 , NM_166914.2
Accession Version	NM_130603.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ER degradation enhancer, mannosidase alpha-like 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_130603.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA 
CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC 
TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC 
TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC 
CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC 
GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG 
CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG 
GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC 
ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC 
GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC 
GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT 
TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA 
GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA 
GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG 
GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC 
CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC 
ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC 
ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC 
GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG 
CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC 
AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC 
AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC 
GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC 
ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC 
ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA 
AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG 
TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC 
GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA 
CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA 
AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT 
GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA 
AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC 
GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG 
AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT 
CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA 
GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC 
GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA 
AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT 
ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA 
GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC 
CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT 
CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC 
TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT 
GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG 
CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG 
GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC 
CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG 
TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA 
GCGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569959.1
CDS	54..3002
Translation	MQQKPRNNGYPGTLICAVLLVLLSTGVSGQKQYSKARKLELREDVRRMFQHAYDGYLRHASNYDELRPLTCDGHDTWGSYSLTLIDALDTLATMGNFTEFRRAARLLEEKMDFDRDINVSVFETNIRIVGGLLSAHLLSKRAGVELEPGWPCKGPLLRLAEDVARRLLPAFVTNTGMPYGTVNLRYGVPKGETSITCTAGVGTFLIEFGTLSRLTGKTIYEDVAMKAVHALWAYRSPIGLFGNHIDVQSGRWTALDSGIGAGVDSLFEYLVKASVLLNRPELLELFHEARAAIDKYMRKEDWYVWVGMNKGRVTLPVFQSLEAFWPGILSIIGDTEPALRTISRYIGVWKKYGFLPEFYNIAAGEASPNREVYPLRPELIESAMYLYRATGNEYLLEFGEHMLETLEFSAKTKCGYATIRNVVTHEKENRMESFFLAETSKYLYLLFDEENFLHNDGSGGELLSTEDDVCVVQAGAYIFNTEAHPMDMSALHCCHAHNEDIYTSLDLQRFSPRAILERSKKRQVAVQEQWVPQCHPENNEFFNKEDESVQAQDSEKEQEREQSGTSTTMAVDIEVFDEFQQPAGDLLVSNFEQIREERELNESLHRNVVARNQLTVSDLDEFFAQRRESFASASEALNYVKTFMGNYTMDVAFIRGLQLYDTNMSSVLGTGAQKEYESRMRTLWQLYELQQQYVANIRVIQGLGLLTFPADGDRIPSHLTEVLDSLDKSAHLEHMDLQDLETRVSNTSSPQLAHIREVILKARNAYAIAMVNTSAMQEFAIRIYLSGTSEAGVRIHPLLEDGELSEEARKRPLKLAEERALFSYARRIVDFRKRMAETVDRLQTLMQDIPPPKRKEATTDSQAADASTVYTQTKAQMAAPAQQPEPSQEEKQSTQIQKGKLAENGSGLGEEESGCVWSQLVQTILRKTTVQRVKFDEAVLQENTRKALEKYARKELPHHLFACHRPEYIEGFAYRDFYPEAL

Target ORF information:

RefSeq Version	NM_130603.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130603.3

ORF Insert Sequence:

ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA 
CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC 
TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC 
TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC 
CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC 
GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG 
CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG 
GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC 
ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC 
GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC 
GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT 
TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA 
GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA 
GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG 
GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC 
CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC 
ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC 
ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC 
GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG 
CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC 
AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC 
AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC 
GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC 
ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC 
ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA 
AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG 
TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC 
GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA 
CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA 
AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT 
GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA 
AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC 
GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG 
AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT 
CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA 
GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC 
GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA 
AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT 
ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA 
GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC 
CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT 
CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC 
TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT 
GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG 
CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG 
GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC 
CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG 
TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA 
GCGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Edem1 ( NM_001258554.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245483.1 Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa00531
Clone ID Related Accession (Same CDS sequence)	NM_166915.2 , NM_130603.3 , NM_001258554.2 , NM_166914.2
Accession Version	NM_001258554.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ER degradation enhancer, mannosidase alpha-like 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001258554.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA 
CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC 
TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC 
TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC 
CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC 
GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG 
CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG 
GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC 
ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC 
GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC 
GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT 
TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA 
GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA 
GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG 
GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC 
CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC 
ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC 
ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC 
GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG 
CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC 
AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC 
AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC 
GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC 
ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC 
ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA 
AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG 
TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC 
GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA 
CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA 
AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT 
GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA 
AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC 
GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG 
AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT 
CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA 
GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC 
GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA 
AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT 
ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA 
GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC 
CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT 
CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC 
TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT 
GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG 
CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG 
GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC 
CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG 
TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA 
GCGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245483.1
CDS	361..3309
Translation	MQQKPRNNGYPGTLICAVLLVLLSTGVSGQKQYSKARKLELREDVRRMFQHAYDGYLRHASNYDELRPLTCDGHDTWGSYSLTLIDALDTLATMGNFTEFRRAARLLEEKMDFDRDINVSVFETNIRIVGGLLSAHLLSKRAGVELEPGWPCKGPLLRLAEDVARRLLPAFVTNTGMPYGTVNLRYGVPKGETSITCTAGVGTFLIEFGTLSRLTGKTIYEDVAMKAVHALWAYRSPIGLFGNHIDVQSGRWTALDSGIGAGVDSLFEYLVKASVLLNRPELLELFHEARAAIDKYMRKEDWYVWVGMNKGRVTLPVFQSLEAFWPGILSIIGDTEPALRTISRYIGVWKKYGFLPEFYNIAAGEASPNREVYPLRPELIESAMYLYRATGNEYLLEFGEHMLETLEFSAKTKCGYATIRNVVTHEKENRMESFFLAETSKYLYLLFDEENFLHNDGSGGELLSTEDDVCVVQAGAYIFNTEAHPMDMSALHCCHAHNEDIYTSLDLQRFSPRAILERSKKRQVAVQEQWVPQCHPENNEFFNKEDESVQAQDSEKEQEREQSGTSTTMAVDIEVFDEFQQPAGDLLVSNFEQIREERELNESLHRNVVARNQLTVSDLDEFFAQRRESFASASEALNYVKTFMGNYTMDVAFIRGLQLYDTNMSSVLGTGAQKEYESRMRTLWQLYELQQQYVANIRVIQGLGLLTFPADGDRIPSHLTEVLDSLDKSAHLEHMDLQDLETRVSNTSSPQLAHIREVILKARNAYAIAMVNTSAMQEFAIRIYLSGTSEAGVRIHPLLEDGELSEEARKRPLKLAEERALFSYARRIVDFRKRMAETVDRLQTLMQDIPPPKRKEATTDSQAADASTVYTQTKAQMAAPAQQPEPSQEEKQSTQIQKGKLAENGSGLGEEESGCVWSQLVQTILRKTTVQRVKFDEAVLQENTRKALEKYARKELPHHLFACHRPEYIEGFAYRDFYPEAL

Target ORF information:

RefSeq Version	NM_001258554.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258554.2

ORF Insert Sequence:

ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA 
CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC 
TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC 
TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC 
CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC 
GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG 
CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG 
GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC 
ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC 
GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC 
GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT 
TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA 
GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA 
GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG 
GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC 
CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC 
ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC 
ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC 
GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG 
CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC 
AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC 
AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC 
GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC 
ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC 
ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA 
AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG 
TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC 
GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA 
CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA 
AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT 
GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA 
AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC 
GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG 
AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT 
CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA 
GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC 
GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA 
AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT 
ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA 
GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC 
CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT 
CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC 
TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT 
GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG 
CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG 
GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC 
CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG 
TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA 
GCGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Edem1 ( NM_166914.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726777.1 Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00531
Clone ID Related Accession (Same CDS sequence)	NM_166915.2 , NM_130603.3 , NM_001258554.2 , NM_166914.2
Accession Version	NM_166914.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ER degradation enhancer, mannosidase alpha-like 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_166914.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA 
CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC 
TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC 
TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC 
CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC 
GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG 
CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG 
GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC 
ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC 
GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC 
GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT 
TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA 
GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA 
GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG 
GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC 
CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC 
ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC 
ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC 
GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG 
CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC 
AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC 
AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC 
GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC 
ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC 
ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA 
AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG 
TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC 
GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA 
CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA 
AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT 
GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA 
AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC 
GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG 
AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT 
CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA 
GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC 
GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA 
AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT 
ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA 
GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC 
CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT 
CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC 
TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT 
GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG 
CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG 
GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC 
CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG 
TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA 
GCGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726777.1
CDS	503..3451
Translation	MQQKPRNNGYPGTLICAVLLVLLSTGVSGQKQYSKARKLELREDVRRMFQHAYDGYLRHASNYDELRPLTCDGHDTWGSYSLTLIDALDTLATMGNFTEFRRAARLLEEKMDFDRDINVSVFETNIRIVGGLLSAHLLSKRAGVELEPGWPCKGPLLRLAEDVARRLLPAFVTNTGMPYGTVNLRYGVPKGETSITCTAGVGTFLIEFGTLSRLTGKTIYEDVAMKAVHALWAYRSPIGLFGNHIDVQSGRWTALDSGIGAGVDSLFEYLVKASVLLNRPELLELFHEARAAIDKYMRKEDWYVWVGMNKGRVTLPVFQSLEAFWPGILSIIGDTEPALRTISRYIGVWKKYGFLPEFYNIAAGEASPNREVYPLRPELIESAMYLYRATGNEYLLEFGEHMLETLEFSAKTKCGYATIRNVVTHEKENRMESFFLAETSKYLYLLFDEENFLHNDGSGGELLSTEDDVCVVQAGAYIFNTEAHPMDMSALHCCHAHNEDIYTSLDLQRFSPRAILERSKKRQVAVQEQWVPQCHPENNEFFNKEDESVQAQDSEKEQEREQSGTSTTMAVDIEVFDEFQQPAGDLLVSNFEQIREERELNESLHRNVVARNQLTVSDLDEFFAQRRESFASASEALNYVKTFMGNYTMDVAFIRGLQLYDTNMSSVLGTGAQKEYESRMRTLWQLYELQQQYVANIRVIQGLGLLTFPADGDRIPSHLTEVLDSLDKSAHLEHMDLQDLETRVSNTSSPQLAHIREVILKARNAYAIAMVNTSAMQEFAIRIYLSGTSEAGVRIHPLLEDGELSEEARKRPLKLAEERALFSYARRIVDFRKRMAETVDRLQTLMQDIPPPKRKEATTDSQAADASTVYTQTKAQMAAPAQQPEPSQEEKQSTQIQKGKLAENGSGLGEEESGCVWSQLVQTILRKTTVQRVKFDEAVLQENTRKALEKYARKELPHHLFACHRPEYIEGFAYRDFYPEAL

Target ORF information:

RefSeq Version	NM_166914.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166914.2

ORF Insert Sequence:

ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA 
CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC 
TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC 
TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC 
CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC 
GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG 
CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG 
GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC 
ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC 
GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC 
GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT 
TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA 
GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA 
GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG 
GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC 
CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC 
ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC 
ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC 
GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG 
CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC 
AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC 
AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC 
GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC 
ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC 
ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA 
AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG 
TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC 
GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA 
CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA 
AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT 
GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA 
AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC 
GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG 
AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT 
CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA 
GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC 
GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA 
AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT 
ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA 
GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC 
CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT 
CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC 
TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT 
GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG 
CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG 
GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC 
CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG 
TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA 
GCGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Edem1 ( NM_001272223.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259152.1 Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant E, mRNA.

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CloneID	OFa03766
Clone ID Related Accession (Same CDS sequence)	NM_001272223.2
Accession Version	NM_001272223.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ER degradation enhancer, mannosidase alpha-like 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272223.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA 
CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC 
TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC 
TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC 
CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC 
GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG 
CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG 
GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC 
ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC 
GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC 
GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT 
TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA 
GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA 
GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG 
GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC 
CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC 
ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC 
ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC 
GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG 
CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC 
AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC 
AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC 
GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC 
ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC 
ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA 
AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG 
TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC 
GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA 
CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA 
AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT 
GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA 
AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC 
GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG 
AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT 
CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA 
GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC 
GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA 
AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT 
ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA 
GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC 
CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT 
CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC 
TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT 
GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG 
CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG 
GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC 
CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG 
TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA 
GCGCTCAAAG TTCGGCGTCG ATGCGCGCAT GACAATGAGC GACTCAATTG CAGAGCGAAA 
AGGACAGAGC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259152.1
CDS	361..3375
Translation	MQQKPRNNGYPGTLICAVLLVLLSTGVSGQKQYSKARKLELREDVRRMFQHAYDGYLRHASNYDELRPLTCDGHDTWGSYSLTLIDALDTLATMGNFTEFRRAARLLEEKMDFDRDINVSVFETNIRIVGGLLSAHLLSKRAGVELEPGWPCKGPLLRLAEDVARRLLPAFVTNTGMPYGTVNLRYGVPKGETSITCTAGVGTFLIEFGTLSRLTGKTIYEDVAMKAVHALWAYRSPIGLFGNHIDVQSGRWTALDSGIGAGVDSLFEYLVKASVLLNRPELLELFHEARAAIDKYMRKEDWYVWVGMNKGRVTLPVFQSLEAFWPGILSIIGDTEPALRTISRYIGVWKKYGFLPEFYNIAAGEASPNREVYPLRPELIESAMYLYRATGNEYLLEFGEHMLETLEFSAKTKCGYATIRNVVTHEKENRMESFFLAETSKYLYLLFDEENFLHNDGSGGELLSTEDDVCVVQAGAYIFNTEAHPMDMSALHCCHAHNEDIYTSLDLQRFSPRAILERSKKRQVAVQEQWVPQCHPENNEFFNKEDESVQAQDSEKEQEREQSGTSTTMAVDIEVFDEFQQPAGDLLVSNFEQIREERELNESLHRNVVARNQLTVSDLDEFFAQRRESFASASEALNYVKTFMGNYTMDVAFIRGLQLYDTNMSSVLGTGAQKEYESRMRTLWQLYELQQQYVANIRVIQGLGLLTFPADGDRIPSHLTEVLDSLDKSAHLEHMDLQDLETRVSNTSSPQLAHIREVILKARNAYAIAMVNTSAMQEFAIRIYLSGTSEAGVRIHPLLEDGELSEEARKRPLKLAEERALFSYARRIVDFRKRMAETVDRLQTLMQDIPPPKRKEATTDSQAADASTVYTQTKAQMAAPAQQPEPSQEEKQSTQIQKGKLAENGSGLGEEESGCVWSQLVQTILRKTTVQRVKFDEAVLQENTRKALEKYARKELPHHLFACHRPEYIEGFAYRDFYPEALKVRRRCAHDNERLNCRAKRTER

Target ORF information:

RefSeq Version	NM_001272223.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ER degradation enhancer, mannosidase alpha-like 1 (Edem1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272223.2

ORF Insert Sequence:

ATGCAGCAAA AACCCCGAAA CAACGGCTAT CCGGGCACCC TCATCTGCGC GGTCCTCCTC 
GTCCTGCTGT CCACCGGTGT CAGCGGCCAG AAGCAGTATT CCAAGGCAAG AAAGTTGGAA 
CTGAGGGAGG ATGTGCGGCG CATGTTCCAG CACGCCTACG ATGGTTACCT GCGCCACGCC 
TCCAACTACG ATGAACTGCG TCCGCTGACC TGCGATGGCC ACGACACCTG GGGCAGCTAC 
TCCTTGACAC TGATCGACGC TCTGGACACG CTGGCCACCA TGGGCAACTT CACGGAATTC 
CGCCGCGCGG CAAGACTGCT CGAGGAGAAG ATGGATTTTG ACAGGGACAT CAACGTGTCC 
GTGTTCGAGA CAAACATCCG CATCGTGGGC GGGCTGCTGT CCGCGCATCT GCTCTCCAAG 
CGTGCTGGCG TAGAGCTAGA GCCAGGATGG CCCTGCAAGG GACCTCTGCT GCGACTGGCG 
GAGGATGTGG CCCGACGATT ATTGCCAGCC TTCGTCACGA ACACGGGTAT GCCTTATGGC 
ACGGTGAACC TGCGCTATGG CGTGCCCAAG GGCGAGACCT CTATAACCTG TACGGCGGGC 
GTGGGGACAT TCCTCATCGA ATTTGGTACC CTTAGCCGGT TGACCGGGAA AACCATCTAC 
GAGGATGTTG CTATGAAGGC GGTACATGCT CTATGGGCAT ATCGCTCGCC CATTGGACTT 
TTTGGCAATC ACATCGACGT GCAGAGCGGT CGTTGGACCG CCCTCGACTC TGGTATCGGA 
GCAGGAGTGG ATTCCCTTTT TGAATACCTA GTTAAGGCAT CTGTCCTGCT CAATCGCCCA 
GAACTCTTGG AGTTATTCCA TGAGGCTCGC GCGGCCATTG ATAAGTACAT GCGGAAGGAG 
GACTGGTACG TGTGGGTAGG CATGAACAAA GGCCGCGTCA CCCTTCCCGT CTTTCAGTCC 
CTGGAGGCGT TTTGGCCCGG CATCCTGAGC ATTATTGGCG ACACGGAGCC GGCCTTGCGC 
ACCATATCAC GGTACATTGG GGTCTGGAAG AAGTACGGCT TTCTGCCAGA GTTTTATAAC 
ATTGCTGCTG GAGAGGCATC GCCGAATCGG GAGGTGTACC CCCTGCGACC GGAGCTAATC 
GAGTCAGCTA TGTATCTCTA TCGAGCCACA GGAAACGAGT ATCTTCTGGA GTTTGGTGAG 
CACATGCTGG AGACCTTGGA GTTCAGTGCC AAAACCAAAT GCGGATATGC AACGATTCGC 
AATGTTGTCA CCCACGAAAA GGAGAATCGC ATGGAGTCAT TCTTCCTGGC GGAAACAAGC 
AAGTACCTTT ATCTGCTGTT TGACGAGGAA AACTTCCTGC ACAACGATGG GTCCGGAGGC 
GAGCTGCTGT CAACCGAGGA TGATGTATGC GTGGTACAGG CCGGTGCGTA CATCTTCAAC 
ACAGAGGCTC ATCCCATGGA CATGTCAGCC CTCCACTGTT GCCACGCCCA CAACGAGGAC 
ATCTACACCT CTCTGGATCT ACAGCGGTTT AGTCCACGGG CGATATTAGA GAGGAGCAAA 
AAGCGGCAGG TAGCTGTTCA GGAACAGTGG GTGCCGCAGT GTCACCCAGA GAACAACGAG 
TTCTTTAATA AAGAGGATGA GAGTGTGCAG GCGCAGGACT CTGAAAAAGA GCAGGAACGC 
GAGCAATCCG GCACAAGCAC TACCATGGCC GTAGATATTG AGGTGTTCGA CGAATTTCAA 
CAGCCCGCCG GCGATCTGTT AGTAAGCAAC TTCGAGCAGA TACGCGAGGA GCGGGAGCTA 
AACGAGAGCC TGCACAGAAA CGTGGTGGCA CGAAACCAGT TGACAGTCAG CGATTTGGAT 
GAATTCTTTG CTCAGCGACG GGAGAGTTTC GCAAGCGCCT CCGAGGCTCT TAACTATGTA 
AAGACCTTCA TGGGCAACTA TACCATGGAT GTAGCCTTTA TACGGGGCTT GCAACTGTAC 
GATACTAATA TGAGTAGTGT ATTGGGCACA GGCGCCCAGA AAGAGTATGA GTCTCGAATG 
AGGACCCTTT GGCAGCTGTA CGAACTGCAG CAGCAATATG TGGCCAACAT CCGAGTGATT 
CAAGGATTAG GTTTGCTAAC CTTTCCGGCG GACGGTGACC GGATACCTAG CCATTTGACA 
GAGGTGTTGG ACAGCCTGGA TAAGTCGGCT CACTTGGAGC ATATGGATCT ACAAGATCTC 
GAAACAAGAG TGTCCAATAC CTCCTCGCCC CAGTTGGCTC ATATAAGAGA AGTTATTCTA 
AAAGCACGTA ATGCCTATGC CATAGCCATG GTCAATACTT CGGCCATGCA GGAATTTGCT 
ATCCGAATCT ACCTCAGTGG CACTTCGGAG GCCGGTGTTC GCATTCACCC ACTTTTGGAA 
GACGGAGAAC TAAGTGAGGA AGCACGTAAA AGGCCATTAA AGTTGGCGGA GGAGCGAGCC 
CTCTTCAGCT ACGCCCGTCG AATAGTGGAC TTTCGGAAAC GAATGGCGGA GACAGTTGAT 
CGATTGCAGA CGCTCATGCA GGACATTCCG CCACCCAAAA GGAAAGAGGC CACTACCGAC 
TCTCAGGCCG CAGATGCGTC CACAGTCTAC ACTCAAACCA AAGCCCAGAT GGCGGCGCCT 
GCACAGCAGC CGGAGCCGAG CCAGGAAGAG AAGCAGTCGA CACAAATTCA AAAAGGAAAG 
CTTGCCGAAA ACGGCTCCGG ATTGGGTGAA GAGGAGTCCG GCTGCGTGTG GTCGCAGTTG 
GTGCAGACCA TCCTTCGGAA GACCACGGTG CAGCGGGTCA AATTTGACGA GGCCGTGCTC 
CAGGAAAATA CGCGCAAGGC ACTGGAGAAG TACGCCCGCA AGGAGTTACC CCACCATCTG 
TTCGCCTGCC ACCGGCCGGA GTACATTGAG GGATTCGCCT ATCGGGACTT TTATCCGGAA 
GCGCTCAAAG TTCGGCGTCG ATGCGCGCAT GACAATGAGC GACTCAATTG CAGAGCGAAA 
AGGACAGAGC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Edem1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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