trr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	trr
Entrez Gene ID	31149
Full Name	trithorax-related
Synonyms	CG3848,Dmel\CG3848,EG:63B12.3,TRR,Trr,anon-2Bb
General protein information	Preferred Names trithorax-related Names CG3848 gene product from transcript CG3848-RC CG3848-PC CG3848-PD Trithorax-related trithorax related trithorax-related protein trr-PC trr-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2B14-2B14

mRNA and Protein(s)

mRNA	Protein	Name
NM_166911.4	NP_726773.2	trithorax-related, isoform D
NM_080301.3	NP_525040.2	trithorax-related, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Histone H3K4 monomethylation catalyzed by Trr and mammalian MML3/MLL4 proteins at enhancers is dispensable for development and viability.
Title: Histone H3K4 monomethylation catalyzed by Trr and mammalian COMPASS-like proteins at enhancers is dispensable for development and viability.

trr and G9a also have common direct targets, including the Drosophila ortholog of Arc (Arc1), a key regulator of synaptic plasticity. Our data highlight the clinical and molecular convergence between the KMT2 and EHMT protein families, which may contribute to a molecular network underlying a larger group of intellectual disability / autism spectrum disorder -related disorders.
Title: Functional convergence of histone methyltransferases EHMT1 and KMT2C involved in intellectual disability and autism spectrum disorder.

trr mutant cells have a growth advantage over their wild-type neighbors and display changes in the levels of multiple proteins that regulate growth and cell division, including Notch, Capicua, and cyclin B.
Title: The Drosophila ortholog of MLL3 and MLL4, trithorax related, functions as a negative regulator of tissue growth.

Ash2 functions together with Trr as an ecdysone receptor coactivator.
Title: Ash2 acts as an ecdysone receptor coactivator by stabilizing the histone methyltransferase Trr.

Trr, the Drosophila homolog of the mammalian Mll3/4 COMPASS-like complexes, can function as a major H3K4 monomethyltransferase on enhancers in vivo.
Title: Enhancer-associated H3K4 monomethylation by Trithorax-related, the Drosophila homolog of mammalian Mll3/Mll4.

The following trr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00524	NM_080301.3 Latest version!	Drosophila melanogaster trithorax-related (trr), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OFa00525	NM_166911.4 Latest version!	Drosophila melanogaster trithorax-related (trr), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trr ( NM_080301.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525040.2 Drosophila melanogaster trithorax-related (trr), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00524
Clone ID Related Accession (Same CDS sequence)	NM_080301.3
Accession Version	NM_080301.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trithorax-related, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080301.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATAC CGAAGGTGAC AACATCGCTT GGTGCCGCCG AAAAGGCGAA ACCCGAAAGA 
GTAGCATCCG TGGCCGCCGC TGCCTTCAAT GCGGTCAGCC TGCAGAAGCG CAGCGGCGAC 
GACACCGCCA CTCCGGCGGA GGATCCCACG CGCAAGAAGG CCAAGACGGA GCTGCTTCTC 
GGAACTGGAA CGGCAGCGCC CAGTTTGCCC GCCAAAGCGT CGAGCACCGC GCCGCAGCAG 
CTCCTCTACC AGCGGAGTGG ACAGCAGGCT AAGGCGCAGG TAAAAGCTGC GTCGGAGCCG 
CAGGATGTCG AGACGGCGGA CGGCGTCTGG GACGCCCGCG ATCAGCAGAT CATAGTGTGC 
AACTTCGGCT CCGGCACTGA GATGGGTGCC ATCAAGGCGG AGGACGCTGA CAAGCAGACC 
TTTACCAGCT TTACCAAAAA GGAGGGTGCC TCATCGTCGG CCTCGTCATC ATCGTCAACC 
GCGTCCGTCA TTTCCATCGA GCCCAGTGGC TCGGGGCAGG ATCATGCTGA AAACTCCGGC 
AAATCTGAGG ACCTCGACTA CGTACTGATG CCAGCCAGCG GCGCGGATAG CTCTACATCT 
GTGGGTAACT CCACCGGAAC GGGAACACCA GCAGGAACGC CTATTGGAGC TACTACCTCC 
ACCATTATTT TAAACGCTAA CAACGGAACG GCAGGAGTCA GTGGAGCCGG CACCACTACA 
ATCCTCACAC AGAAATCCGG TCACACGAAC TACAACATCT TCAATACCAC GGCCACTGGT 
AGCCAAACGC CCACTACAAC ATTGTTAAAC CGGGTGAATC TTCACCCCAA GATGAAAACC 
CAGCTGATGG TTAACGCCAA GAAGCTGTCG GAAGTAACGC AGACCACGGC GAAAGTGTCG 
ATTGGCAACA AGACTATATC GGTGCCGCTG CTCAAGCCGC TGATGAGCGC TAGTGGAGCG 
GCCACAGCCG GAGGAGCCAC GATTGTAGAG AGCAAGCAAC TGCTGCAGCC GGGCGGTCAG 
GTGACTACCG TAATGAGTGC TGCGCAGCAA AGCGGTGGTC AGCAGGTCCA CCCGCACGTC 
CATTCTCATG CACATCACAA CTTTACCAAA CTTATCAAAC GCGGGCCAAA AAATAGCGGC 
ACCATTGTCT CGTTTTCGGG GCTACAGATC AAGCCGGCGA ACACCAAAAT TGTGGCCACC 
AAGGTGGTGA GCAAAAAGAT GCTGCAACTT CAGCAGCACC AACAACAGAT CCAACAGCAG 
CAGCAGCTTC AGCAGCTGCA GGTGACCAGT GGTGGAGGCC TGGCTCCGCC CACAGGCAGC 
ATAGTGACTA TCACCACCAC CAACCCCAGC CAAACGTACG CAATGGTTCA GGATTCCGCA 
ACTGTGGGAC CTGCGGCCCA TTCGGAAGAC GATGCGCCAG CGCCTCGAAA GATTACAGCC 
TACTCGGAGA ACCTTCAAAA AATTCTCAAC AAGAGCAAGT CCCAGGAATC GACTGGAGGC 
CCGGAGGAGT TCACCAACAT CAACAGCGTG GTGATTAAGC CGCTCGACAA GAATACACTC 
AATTGTCCAC CCAGCTTTAA CATCTTTAAG CAGCAGCAGC ACTCACAGGC TGCTCAAAGC 
CAATCAATCT CCGCGGTAGG CAGTGGAGCA GGCACACCCG TGACGTTTAC CATGGCTTCG 
GGGAATGCCT CAGATCTGGC CACCACCTCC ACCGTTTCCG TTTCGGCGGG GACCATCTGC 
ATAAACAGTC CGATGATGGG CACGCGGCCA ATAATCTCCA TTCAGAACAA GAACATATCC 
CTGGTGCTGT CCAAGACGAC CATGGCCCAG CAAAAACCGA AGATGATAAC TACAACCACG 
TTGTCCAGTC AGGCGGCGCT GCAGATGCAT CATGCTTTGA TTCAGGATTC TAGTGCAGAC 
AAGGCGGGAT CTTCTGCAAA CTCCGGATCA GCTACCTCGG GAGCTTCAAT GCAGCTGAAA 
CTAACGACCG CCAATACGCC CACCAAACTC AGCGTTAGTC TGGCTCCGGA CGTAGTCAAG 
CTGGAAGAAG TTGGCAGCGA ATCTAAAGCA AAGCTACTGG TCAAACAGGA GGCGGTTGTG 
AAGGATAGCA CTGGTACTCC GACCAGTGAG GAGCGGGCTG AGGAGATCGG CACGCCGGAG 
AAGCGTTTGA ACGCCAATGC CACAATGACG GCTATTAACC AGGTTCAAAA TCAGTCCGCC 
AATCAAATTC AAATGGCCAC ATCTACCTCA ACTGCCTCGA ATCCCAGCAC ACCCAATCCT 
ACAGTGAACG CCACACCTAT GAACAACCAA CGCTCGGCAG CGGAGGATAA TGCACTTCTA 
AAGCAACTGC TCCAAAACAA CAGCAGCAGT CATAGCCTCA ACCAGATAAG CATCACTTCG 
GCTCACGTTG GTTCCGCCTC AGCTTCGGCA CCTCTTTCCG CCCGCAAGGT TATCAACGTA 
CGGGCGCCCA GCATGGGGAA GGTCAGAAGT TTGGAGGATC AACTGGCACG TCCGGTGATT 
CCTCCGGTGC CCACTGCCAC GCAGGCGGCA GGTAGTAGTA GTAGCAGTGG ATCTGTGGCC 
ACGTCCACCA CCACCACAAC AGTAGCTAGC GGAGGAAGCA GCCAACAGGT AGCCACCGCC 
TCAGCGACGG CTCTACCTGT TAGTGCGGTC GCAATTACCA CACCAGGAGT GGGAGGGGAG 
GCAAAGCTGG AGCAGAAATC CGATCAGCCA GCAGCAATTA TGCAGAATCA GAGCCAAAAC 
CAGGCGCCTC CACCACCACC GCCACCACAG CAGCAGCAGC AGCAGCAACT ACACCAGCCG 
CAGCAGCTGC AACCGTCACC CCACCAAGTC AAGCAGACTG TGCAGATTGT GTCGAAGGAG 
ACGTCGTTCA TCTCAGGACC CGTTGCTGCT AAAACACTAG TGACGGAAGC CACTTCGAAG 
CCGGCTGAAC TACTACCTCC TCCGCCTTAC GAAATGGCCA CTGCTCCTAT ATCGAACGTC 
ACCATATCCA TATCCACCAA ACAAGCGGCT CCGAAGGAAC TGCAGATGAA GCCCAAGGCG 
GTAGCTATGT CACTACCCAT GGAGCAGGGC GACGAATCGC TGCCAGAGCA AGCGGAGCCG 
CCGCTTCATT CGGAACAGGG AGCTACTGCA GCGGGAGTTG CACCTCACTC CGGAGGTCCC 
CTTGTGTCTG CGCAATGGAC CAATAACCAT TTAGAAGGAG GTGTAGCCAC CACAAAGATA 
CCGTTCAAGC CTGGAGAGCC TCAAAAACGC AAGCTCCCCA TGCACCCACA ACTGGACGAA 
AAGCAGATCC AGCAACAGGC AGAGATTCCC ATAAGCACCT CCCTACCAAC AACTCCGACT 
GGACAAGGTA CTCCCGACAA AGTACAGCTG ATCTCAGCGA TTGCCACTTA TGTAAAGAAA 
TCCGGTGTCC CGAACGAGGC ACAACCCATC CAGAACCAGA GCCAGGGCCA GGTGCAGATG 
CAGGCGCAAA TGCAGGCGAC TATGCAAGGA CACCTGTCTG GGCAGATGTC CGGACAAATC 
TCCGGACATG CCGCTGGACA GATACCCGCT CAAATGCATT TGCAAGTACA GCATCAGCTC 
CATATGGCAG TTCATCCACA GCAGCAGCAG CAGCAACTGC ACCAGAACCA ACCACAGAAT 
GCGACGATTC CCCTTCCAGT TACTGGACAG GGTGCAGTGC CCATCCCCGT GCCCACCATG 
GAGAGCAAGG CAGGCGACCA AAGGAAGCGC CGCAAACGTG AGGTCCAGAA GCCGAGGCGC 
ACAAATCTGA ACGCCGGACA AGCGGGTGGA GCTCTTAAGG ATCTAACAGG TCCACTACCG 
GCAGGTGCAA TGGTCCAACT GGCAGGAATG CCGCCGGGGA CCCAGTACAT ACAAGGCGCG 
GCATCAGGGA CTGGTCATGT GATCACCAGT ACCGGACAAG GAGTTACTTT GGGAGGAGTG 
GGCGCCAGTA CTGGAGCCAG TTCCTCGCCC ATGCTCAAGA AGCGGGTTCG CAAGTTCTCC 
AAGGTAGAAG AGGATCACGA TGCATTTACC GAGAAGTTGC TGACGCACAT CCGCCAAATG 
CAGCCGCTTC AAGTTCTGGA GCCGCATCTC AATCGCAACT TTCACTTTCT TATCGGGAGC 
AACGAGACTT CTGGAGGTGG TTCGCCCGCC TCAATGAGTT CTGCGGCTTC TGCCGGATCA 
TCATCGGCTG GAGGAGGAAA GCTGAAGGGC GGATCTCGAG GCTGGCCACT GAGCAGGCAT 
TTGGAAGGTC TGGAGGACTG TGATGGCACC GTTCTCGGCC GCTATGGTCG AGTGAATCTG 
CCAGGGATAC CCTCGTTGTA CGATAGCGAA CGCTTTGGCG GAAGTAGAGG TCTAGTTGGT 
GGATCTGCAC GAACCCGTTC ACCCTCGCCA GCAGAGTCTC CGGGAGCAGA AAAGATGCTA 
CCAATGTCTA GCATCCAAAA TGATTTTTAT GACCAGGAGT TCTCCACGCA TATGGAGCGC 
AATCCTAGGG AACGCCTGGT GCGGCACATT GGAGCCGTCA AGGATTGCAA TTTGGAGACC 
GTAGACCTGG TGGAGAGCGA GGGCGTAGCT GCGTGGGCGA CTCTTCCACG ACTTACCCGC 
TATCCGGGTC TAATACTGCT CAATGGAAAC AGCCGTTGCC ATGGACGGAT GTCGCCGGTC 
GCACTGCCGG AGGATCCGCT GACCATGCGC TTTCCCGTGT CTCCACTGCT TCGATCCTGC 
GGCGAAGAGC TGCGCAAGAC ACAGCAGATG GAGCTGGGAA TGGGACCGCT GGGCAATAAT 
AATAATAACA ATTACCAGCA GAAGAACCAG AATGTGATAC TCGCACTGCC CGCCTCGGCA 
TCGGAGAACA TAGCCGGCGT GCTGCGGGAT CTGGCCAATC TACTGCACTT GGCGCCCGCC 
CTTACCTGCA AGATCATCGA GGACAAGATT GGCAACAAGT TGGAGGACCA GTTTATGAAT 
CAGGACGATG AAAAGCACGT CGACTTCAAG CGACCACTGA GTCAAGTGAG TCACGGTCAT 
CTGAGGAAGA TCCTCAACGG GCGTCGTAAG CTCTGCCGCA GCTGCGGAAA TGTGGTTCAC 
GCCACTGGGC TGCGAGTTCC TCGGCACAGT GTTCCTGCCC TAGAAGAGCA ACTCCCCCGG 
TTGGCTCAAC TGATGGACAT GCTGCCAAGA AAATCGGTTC CCCCTCCTTT TGTGTACTTC 
TGCGATCGCG CCTGCTTTGC GAGATTCAAG TGGAATGGAA AGGATGGCCA GGCGGAGGCG 
GCTAGTCTGC TGCTTCAGCC AGCCGGCGGT TCTGCTGTAA AATCATCCAA CGGAGACTCG 
CCTGGCAGCT TCTGCGCCAG CTCAACTGCC CCAGCGGAAA TGGTGGTCAA ACAAGAACCG 
GAGGACGAGG ATGAGAAAAC GCCGAGCGTA CCGGGCAACC CCACAAATAT CCCCGCACAG 
CGCAAATGCA TTGTGAAGTG TTTTAGTGCT GATTGCTTCA CCACGGACTC GGCGCCATCC 
GGACTGGAGC TCGACGGAAC TGCTGGTGCC GGAACTGGGG CTGGACCGGT CAATAATACT 
GTGTGGGAAA CAGAGACCAG TGGGCTGCAG TTGGAGGACA CGCGGCAGTG TGTCTTCTGC 
AATCAGCGTG GTGACGGCCA GGCAGATGGA CCCTCGCGAC TGCTTAACTT CGATGTGGAT 
AAATGGGTTC ACCTTAACTG CGCCCTGTGG TCCAACGGCG TGTACGAGAC GGTGTCTGGG 
GCGCTGATGA ACTTCCAGAC AGCTCTGCAA GCGGGACTGA GCCAGGCGTG TAGCGCTTGT 
CACCAACCAG GCGCCACCAT CAAGTGTTTC AAGTCGCGCT GCAACAGCCT GTACCACCTG 
CCATGCGCCA TTCGCGAGGA GTGCGTCTTT TACAAGAACA AGTCCGTGCA CTGCAGCGTT 
CATGGTCATG CTCATGCCGG CATCACGATG GGTGCAGGAG CAGGCGCCAC GACTGGCGCC 
GGATTAGGAG GCTCTGTGGC AGATAATGAA CTGAGCTCCC TGGTTGTCCA CCGGAGAGTT 
TTTGTCGATC GGGATGAAAA CCGTCAGGTG GCCACCGTCA TGCACTATTC AGAGCTAAGC 
AACCTGCTCC GTGTGGGCAA CATGACGTTC CTGAACGTGG GTCAGTTACT GCCGCATCAG 
CTGGAGGCCT TCCACACACC CCACTACATC TACCCCATTG GCTACAAGGT GAGTCGCTAC 
TACTGGTGCG TGCGACGACC CAATCGTAGG TGTCGATACA TTTGCAGTAT CGCAGAGGCC 
GGATGCAAGC CCGAGTTTCG CATCCAGGTG CAGGACGCCG GCGACAAGGA GCCGGAGCGC 
GAGTTTCGGG GCAGTTCGCC CTCGGCGGTC TGGCAGCAGA TTCTGCAGCC AATCACGCGA 
CTGCGTAAGG TGCACAAGTG GTTGCAGCTC TTTCCCCAGC ACATCAGTGG TGAGGACCTA 
TTTGGCCTAA CGGAACCGGC CATTGTGCGA ATCCTGGAAA GCCTCCCGGG CATTGAGACG 
CTCACTGACT ACCGCTTCAA GTACGGTCGC AACCCGCTTC TTGAGTTCCC GCTAGCCATC 
AATCCGTCTG GAGCGGCGCG TACGGAACCC AAGCAACGAC AGCTGCTCGT CTGGAGGAAG 
CCGCATACGC AACGCACGGC CGGCAGCTGC AGCACGCAGC GGATGGCCAA CTCGGCTGCG 
ATTGCCGGAG AAGTGGCCTG CCCGTACAGC AAGCAGTTCG TGCATTCGAA GAGCTCCCAG 
TACAAAAAGA TGAAGCAGGA GTGGCGCAAC AACGTCTACT TGGCGAGATC TAAGATCCAG 
GGTCTTGGCT TGTATGCAGC TCGGGACATT GAAAAGCACA CCATGATAAT CGAGTACATT 
GGCGAGGTTA TCCGCACCGA GGTCTCCGAG ATACGCGAGA AGCAGTACGA GTCCAAGAAC 
CGTGGCATCT ACATGTTCCG GCTGGATGAA GACCGCGTGG TGGACGCCAC TCTAAGCGGC 
GGGTTGGCGC GCTACATCAA CCATTCGTGC AATCCCAACT GTGTTACCGA GATAGTGGAG 
GTCGATCGCG ACGTGCGGAT CATAATATTC GCCAAGCGGA AGATCTATCG CGGCGAGGAG 
CTCTCGTACG ACTACAAGTT CGACATTGAG GACGAGTCGC ACAAGATACC GTGCGCCTGT 
GGAGCGCCCA ACTGTCGCAA GTGGATGAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525040.2
CDS	176..7408
Translation	MNIPKVTTSLGAAEKAKPERVASVAAAAFNAVSLQKRSGDDTATPAEDPTRKKAKTELLLGTGTAAPSLPAKASSTAPQQLLYQRSGQQAKAQVKAASEPQDVETADGVWDARDQQIIVCNFGSGTEMGAIKAEDADKQTFTSFTKKEGASSSASSSSSTASVISIEPSGSGQDHAENSGKSEDLDYVLMPASGADSSTSVGNSTGTGTPAGTPIGATTSTIILNANNGTAGVSGAGTTTILTQKSGHTNYNIFNTTATGSQTPTTTLLNRVNLHPKMKTQLMVNAKKLSEVTQTTAKVSIGNKTISVPLLKPLMSASGAATAGGATIVESKQLLQPGGQVTTVMSAAQQSGGQQVHPHVHSHAHHNFTKLIKRGPKNSGTIVSFSGLQIKPANTKIVATKVVSKKMLQLQQHQQQIQQQQQLQQLQVTSGGGLAPPTGSIVTITTTNPSQTYAMVQDSATVGPAAHSEDDAPAPRKITAYSENLQKILNKSKSQESTGGPEEFTNINSVVIKPLDKNTLNCPPSFNIFKQQQHSQAAQSQSISAVGSGAGTPVTFTMASGNASDLATTSTVSVSAGTICINSPMMGTRPIISIQNKNISLVLSKTTMAQQKPKMITTTTLSSQAALQMHHALIQDSSADKAGSSANSGSATSGASMQLKLTTANTPTKLSVSLAPDVVKLEEVGSESKAKLLVKQEAVVKDSTGTPTSEERAEEIGTPEKRLNANATMTAINQVQNQSANQIQMATSTSTASNPSTPNPTVNATPMNNQRSAAEDNALLKQLLQNNSSSHSLNQISITSAHVGSASASAPLSARKVINVRAPSMGKVRSLEDQLARPVIPPVPTATQAAGSSSSSGSVATSTTTTTVASGGSSQQVATASATALPVSAVAITTPGVGGEAKLEQKSDQPAAIMQNQSQNQAPPPPPPPQQQQQQQLHQPQQLQPSPHQVKQTVQIVSKETSFISGPVAAKTLVTEATSKPAELLPPPPYEMATAPISNVTISISTKQAAPKELQMKPKAVAMSLPMEQGDESLPEQAEPPLHSEQGATAAGVAPHSGGPLVSAQWTNNHLEGGVATTKIPFKPGEPQKRKLPMHPQLDEKQIQQQAEIPISTSLPTTPTGQGTPDKVQLISAIATYVKKSGVPNEAQPIQNQSQGQVQMQAQMQATMQGHLSGQMSGQISGHAAGQIPAQMHLQVQHQLHMAVHPQQQQQQLHQNQPQNATIPLPVTGQGAVPIPVPTMESKAGDQRKRRKREVQKPRRTNLNAGQAGGALKDLTGPLPAGAMVQLAGMPPGTQYIQGAASGTGHVITSTGQGVTLGGVGASTGASSSPMLKKRVRKFSKVEEDHDAFTEKLLTHIRQMQPLQVLEPHLNRNFHFLIGSNETSGGGSPASMSSAASAGSSSAGGGKLKGGSRGWPLSRHLEGLEDCDGTVLGRYGRVNLPGIPSLYDSERFGGSRGLVGGSARTRSPSPAESPGAEKMLPMSSIQNDFYDQEFSTHMERNPRERLVRHIGAVKDCNLETVDLVESEGVAAWATLPRLTRYPGLILLNGNSRCHGRMSPVALPEDPLTMRFPVSPLLRSCGEELRKTQQMELGMGPLGNNNNNNYQQKNQNVILALPASASENIAGVLRDLANLLHLAPALTCKIIEDKIGNKLEDQFMNQDDEKHVDFKRPLSQVSHGHLRKILNGRRKLCRSCGNVVHATGLRVPRHSVPALEEQLPRLAQLMDMLPRKSVPPPFVYFCDRACFARFKWNGKDGQAEAASLLLQPAGGSAVKSSNGDSPGSFCASSTAPAEMVVKQEPEDEDEKTPSVPGNPTNIPAQRKCIVKCFSADCFTTDSAPSGLELDGTAGAGTGAGPVNNTVWETETSGLQLEDTRQCVFCNQRGDGQADGPSRLLNFDVDKWVHLNCALWSNGVYETVSGALMNFQTALQAGLSQACSACHQPGATIKCFKSRCNSLYHLPCAIREECVFYKNKSVHCSVHGHAHAGITMGAGAGATTGAGLGGSVADNELSSLVVHRRVFVDRDENRQVATVMHYSELSNLLRVGNMTFLNVGQLLPHQLEAFHTPHYIYPIGYKVSRYYWCVRRPNRRCRYICSIAEAGCKPEFRIQVQDAGDKEPEREFRGSSPSAVWQQILQPITRLRKVHKWLQLFPQHISGEDLFGLTEPAIVRILESLPGIETLTDYRFKYGRNPLLEFPLAINPSGAARTEPKQRQLLVWRKPHTQRTAGSCSTQRMANSAAIAGEVACPYSKQFVHSKSSQYKKMKQEWRNNVYLARSKIQGLGLYAARDIEKHTMIIEYIGEVIRTEVSEIREKQYESKNRGIYMFRLDEDRVVDATLSGGLARYINHSCNPNCVTEIVEVDRDVRIIIFAKRKIYRGEELSYDYKFDIEDESHKIPCACGAPNCRKWMN

Target ORF information:

RefSeq Version	NM_080301.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trithorax-related (trr), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080301.3

ORF Insert Sequence:

ATGAATATAC CGAAGGTGAC AACATCGCTT GGTGCCGCCG AAAAGGCGAA ACCCGAAAGA 
GTAGCATCCG TGGCCGCCGC TGCCTTCAAT GCGGTCAGCC TGCAGAAGCG CAGCGGCGAC 
GACACCGCCA CTCCGGCGGA GGATCCCACG CGCAAGAAGG CCAAGACGGA GCTGCTTCTC 
GGAACTGGAA CGGCAGCGCC CAGTTTGCCC GCCAAAGCGT CGAGCACCGC GCCGCAGCAG 
CTCCTCTACC AGCGGAGTGG ACAGCAGGCT AAGGCGCAGG TAAAAGCTGC GTCGGAGCCG 
CAGGATGTCG AGACGGCGGA CGGCGTCTGG GACGCCCGCG ATCAGCAGAT CATAGTGTGC 
AACTTCGGCT CCGGCACTGA GATGGGTGCC ATCAAGGCGG AGGACGCTGA CAAGCAGACC 
TTTACCAGCT TTACCAAAAA GGAGGGTGCC TCATCGTCGG CCTCGTCATC ATCGTCAACC 
GCGTCCGTCA TTTCCATCGA GCCCAGTGGC TCGGGGCAGG ATCATGCTGA AAACTCCGGC 
AAATCTGAGG ACCTCGACTA CGTACTGATG CCAGCCAGCG GCGCGGATAG CTCTACATCT 
GTGGGTAACT CCACCGGAAC GGGAACACCA GCAGGAACGC CTATTGGAGC TACTACCTCC 
ACCATTATTT TAAACGCTAA CAACGGAACG GCAGGAGTCA GTGGAGCCGG CACCACTACA 
ATCCTCACAC AGAAATCCGG TCACACGAAC TACAACATCT TCAATACCAC GGCCACTGGT 
AGCCAAACGC CCACTACAAC ATTGTTAAAC CGGGTGAATC TTCACCCCAA GATGAAAACC 
CAGCTGATGG TTAACGCCAA GAAGCTGTCG GAAGTAACGC AGACCACGGC GAAAGTGTCG 
ATTGGCAACA AGACTATATC GGTGCCGCTG CTCAAGCCGC TGATGAGCGC TAGTGGAGCG 
GCCACAGCCG GAGGAGCCAC GATTGTAGAG AGCAAGCAAC TGCTGCAGCC GGGCGGTCAG 
GTGACTACCG TAATGAGTGC TGCGCAGCAA AGCGGTGGTC AGCAGGTCCA CCCGCACGTC 
CATTCTCATG CACATCACAA CTTTACCAAA CTTATCAAAC GCGGGCCAAA AAATAGCGGC 
ACCATTGTCT CGTTTTCGGG GCTACAGATC AAGCCGGCGA ACACCAAAAT TGTGGCCACC 
AAGGTGGTGA GCAAAAAGAT GCTGCAACTT CAGCAGCACC AACAACAGAT CCAACAGCAG 
CAGCAGCTTC AGCAGCTGCA GGTGACCAGT GGTGGAGGCC TGGCTCCGCC CACAGGCAGC 
ATAGTGACTA TCACCACCAC CAACCCCAGC CAAACGTACG CAATGGTTCA GGATTCCGCA 
ACTGTGGGAC CTGCGGCCCA TTCGGAAGAC GATGCGCCAG CGCCTCGAAA GATTACAGCC 
TACTCGGAGA ACCTTCAAAA AATTCTCAAC AAGAGCAAGT CCCAGGAATC GACTGGAGGC 
CCGGAGGAGT TCACCAACAT CAACAGCGTG GTGATTAAGC CGCTCGACAA GAATACACTC 
AATTGTCCAC CCAGCTTTAA CATCTTTAAG CAGCAGCAGC ACTCACAGGC TGCTCAAAGC 
CAATCAATCT CCGCGGTAGG CAGTGGAGCA GGCACACCCG TGACGTTTAC CATGGCTTCG 
GGGAATGCCT CAGATCTGGC CACCACCTCC ACCGTTTCCG TTTCGGCGGG GACCATCTGC 
ATAAACAGTC CGATGATGGG CACGCGGCCA ATAATCTCCA TTCAGAACAA GAACATATCC 
CTGGTGCTGT CCAAGACGAC CATGGCCCAG CAAAAACCGA AGATGATAAC TACAACCACG 
TTGTCCAGTC AGGCGGCGCT GCAGATGCAT CATGCTTTGA TTCAGGATTC TAGTGCAGAC 
AAGGCGGGAT CTTCTGCAAA CTCCGGATCA GCTACCTCGG GAGCTTCAAT GCAGCTGAAA 
CTAACGACCG CCAATACGCC CACCAAACTC AGCGTTAGTC TGGCTCCGGA CGTAGTCAAG 
CTGGAAGAAG TTGGCAGCGA ATCTAAAGCA AAGCTACTGG TCAAACAGGA GGCGGTTGTG 
AAGGATAGCA CTGGTACTCC GACCAGTGAG GAGCGGGCTG AGGAGATCGG CACGCCGGAG 
AAGCGTTTGA ACGCCAATGC CACAATGACG GCTATTAACC AGGTTCAAAA TCAGTCCGCC 
AATCAAATTC AAATGGCCAC ATCTACCTCA ACTGCCTCGA ATCCCAGCAC ACCCAATCCT 
ACAGTGAACG CCACACCTAT GAACAACCAA CGCTCGGCAG CGGAGGATAA TGCACTTCTA 
AAGCAACTGC TCCAAAACAA CAGCAGCAGT CATAGCCTCA ACCAGATAAG CATCACTTCG 
GCTCACGTTG GTTCCGCCTC AGCTTCGGCA CCTCTTTCCG CCCGCAAGGT TATCAACGTA 
CGGGCGCCCA GCATGGGGAA GGTCAGAAGT TTGGAGGATC AACTGGCACG TCCGGTGATT 
CCTCCGGTGC CCACTGCCAC GCAGGCGGCA GGTAGTAGTA GTAGCAGTGG ATCTGTGGCC 
ACGTCCACCA CCACCACAAC AGTAGCTAGC GGAGGAAGCA GCCAACAGGT AGCCACCGCC 
TCAGCGACGG CTCTACCTGT TAGTGCGGTC GCAATTACCA CACCAGGAGT GGGAGGGGAG 
GCAAAGCTGG AGCAGAAATC CGATCAGCCA GCAGCAATTA TGCAGAATCA GAGCCAAAAC 
CAGGCGCCTC CACCACCACC GCCACCACAG CAGCAGCAGC AGCAGCAACT ACACCAGCCG 
CAGCAGCTGC AACCGTCACC CCACCAAGTC AAGCAGACTG TGCAGATTGT GTCGAAGGAG 
ACGTCGTTCA TCTCAGGACC CGTTGCTGCT AAAACACTAG TGACGGAAGC CACTTCGAAG 
CCGGCTGAAC TACTACCTCC TCCGCCTTAC GAAATGGCCA CTGCTCCTAT ATCGAACGTC 
ACCATATCCA TATCCACCAA ACAAGCGGCT CCGAAGGAAC TGCAGATGAA GCCCAAGGCG 
GTAGCTATGT CACTACCCAT GGAGCAGGGC GACGAATCGC TGCCAGAGCA AGCGGAGCCG 
CCGCTTCATT CGGAACAGGG AGCTACTGCA GCGGGAGTTG CACCTCACTC CGGAGGTCCC 
CTTGTGTCTG CGCAATGGAC CAATAACCAT TTAGAAGGAG GTGTAGCCAC CACAAAGATA 
CCGTTCAAGC CTGGAGAGCC TCAAAAACGC AAGCTCCCCA TGCACCCACA ACTGGACGAA 
AAGCAGATCC AGCAACAGGC AGAGATTCCC ATAAGCACCT CCCTACCAAC AACTCCGACT 
GGACAAGGTA CTCCCGACAA AGTACAGCTG ATCTCAGCGA TTGCCACTTA TGTAAAGAAA 
TCCGGTGTCC CGAACGAGGC ACAACCCATC CAGAACCAGA GCCAGGGCCA GGTGCAGATG 
CAGGCGCAAA TGCAGGCGAC TATGCAAGGA CACCTGTCTG GGCAGATGTC CGGACAAATC 
TCCGGACATG CCGCTGGACA GATACCCGCT CAAATGCATT TGCAAGTACA GCATCAGCTC 
CATATGGCAG TTCATCCACA GCAGCAGCAG CAGCAACTGC ACCAGAACCA ACCACAGAAT 
GCGACGATTC CCCTTCCAGT TACTGGACAG GGTGCAGTGC CCATCCCCGT GCCCACCATG 
GAGAGCAAGG CAGGCGACCA AAGGAAGCGC CGCAAACGTG AGGTCCAGAA GCCGAGGCGC 
ACAAATCTGA ACGCCGGACA AGCGGGTGGA GCTCTTAAGG ATCTAACAGG TCCACTACCG 
GCAGGTGCAA TGGTCCAACT GGCAGGAATG CCGCCGGGGA CCCAGTACAT ACAAGGCGCG 
GCATCAGGGA CTGGTCATGT GATCACCAGT ACCGGACAAG GAGTTACTTT GGGAGGAGTG 
GGCGCCAGTA CTGGAGCCAG TTCCTCGCCC ATGCTCAAGA AGCGGGTTCG CAAGTTCTCC 
AAGGTAGAAG AGGATCACGA TGCATTTACC GAGAAGTTGC TGACGCACAT CCGCCAAATG 
CAGCCGCTTC AAGTTCTGGA GCCGCATCTC AATCGCAACT TTCACTTTCT TATCGGGAGC 
AACGAGACTT CTGGAGGTGG TTCGCCCGCC TCAATGAGTT CTGCGGCTTC TGCCGGATCA 
TCATCGGCTG GAGGAGGAAA GCTGAAGGGC GGATCTCGAG GCTGGCCACT GAGCAGGCAT 
TTGGAAGGTC TGGAGGACTG TGATGGCACC GTTCTCGGCC GCTATGGTCG AGTGAATCTG 
CCAGGGATAC CCTCGTTGTA CGATAGCGAA CGCTTTGGCG GAAGTAGAGG TCTAGTTGGT 
GGATCTGCAC GAACCCGTTC ACCCTCGCCA GCAGAGTCTC CGGGAGCAGA AAAGATGCTA 
CCAATGTCTA GCATCCAAAA TGATTTTTAT GACCAGGAGT TCTCCACGCA TATGGAGCGC 
AATCCTAGGG AACGCCTGGT GCGGCACATT GGAGCCGTCA AGGATTGCAA TTTGGAGACC 
GTAGACCTGG TGGAGAGCGA GGGCGTAGCT GCGTGGGCGA CTCTTCCACG ACTTACCCGC 
TATCCGGGTC TAATACTGCT CAATGGAAAC AGCCGTTGCC ATGGACGGAT GTCGCCGGTC 
GCACTGCCGG AGGATCCGCT GACCATGCGC TTTCCCGTGT CTCCACTGCT TCGATCCTGC 
GGCGAAGAGC TGCGCAAGAC ACAGCAGATG GAGCTGGGAA TGGGACCGCT GGGCAATAAT 
AATAATAACA ATTACCAGCA GAAGAACCAG AATGTGATAC TCGCACTGCC CGCCTCGGCA 
TCGGAGAACA TAGCCGGCGT GCTGCGGGAT CTGGCCAATC TACTGCACTT GGCGCCCGCC 
CTTACCTGCA AGATCATCGA GGACAAGATT GGCAACAAGT TGGAGGACCA GTTTATGAAT 
CAGGACGATG AAAAGCACGT CGACTTCAAG CGACCACTGA GTCAAGTGAG TCACGGTCAT 
CTGAGGAAGA TCCTCAACGG GCGTCGTAAG CTCTGCCGCA GCTGCGGAAA TGTGGTTCAC 
GCCACTGGGC TGCGAGTTCC TCGGCACAGT GTTCCTGCCC TAGAAGAGCA ACTCCCCCGG 
TTGGCTCAAC TGATGGACAT GCTGCCAAGA AAATCGGTTC CCCCTCCTTT TGTGTACTTC 
TGCGATCGCG CCTGCTTTGC GAGATTCAAG TGGAATGGAA AGGATGGCCA GGCGGAGGCG 
GCTAGTCTGC TGCTTCAGCC AGCCGGCGGT TCTGCTGTAA AATCATCCAA CGGAGACTCG 
CCTGGCAGCT TCTGCGCCAG CTCAACTGCC CCAGCGGAAA TGGTGGTCAA ACAAGAACCG 
GAGGACGAGG ATGAGAAAAC GCCGAGCGTA CCGGGCAACC CCACAAATAT CCCCGCACAG 
CGCAAATGCA TTGTGAAGTG TTTTAGTGCT GATTGCTTCA CCACGGACTC GGCGCCATCC 
GGACTGGAGC TCGACGGAAC TGCTGGTGCC GGAACTGGGG CTGGACCGGT CAATAATACT 
GTGTGGGAAA CAGAGACCAG TGGGCTGCAG TTGGAGGACA CGCGGCAGTG TGTCTTCTGC 
AATCAGCGTG GTGACGGCCA GGCAGATGGA CCCTCGCGAC TGCTTAACTT CGATGTGGAT 
AAATGGGTTC ACCTTAACTG CGCCCTGTGG TCCAACGGCG TGTACGAGAC GGTGTCTGGG 
GCGCTGATGA ACTTCCAGAC AGCTCTGCAA GCGGGACTGA GCCAGGCGTG TAGCGCTTGT 
CACCAACCAG GCGCCACCAT CAAGTGTTTC AAGTCGCGCT GCAACAGCCT GTACCACCTG 
CCATGCGCCA TTCGCGAGGA GTGCGTCTTT TACAAGAACA AGTCCGTGCA CTGCAGCGTT 
CATGGTCATG CTCATGCCGG CATCACGATG GGTGCAGGAG CAGGCGCCAC GACTGGCGCC 
GGATTAGGAG GCTCTGTGGC AGATAATGAA CTGAGCTCCC TGGTTGTCCA CCGGAGAGTT 
TTTGTCGATC GGGATGAAAA CCGTCAGGTG GCCACCGTCA TGCACTATTC AGAGCTAAGC 
AACCTGCTCC GTGTGGGCAA CATGACGTTC CTGAACGTGG GTCAGTTACT GCCGCATCAG 
CTGGAGGCCT TCCACACACC CCACTACATC TACCCCATTG GCTACAAGGT GAGTCGCTAC 
TACTGGTGCG TGCGACGACC CAATCGTAGG TGTCGATACA TTTGCAGTAT CGCAGAGGCC 
GGATGCAAGC CCGAGTTTCG CATCCAGGTG CAGGACGCCG GCGACAAGGA GCCGGAGCGC 
GAGTTTCGGG GCAGTTCGCC CTCGGCGGTC TGGCAGCAGA TTCTGCAGCC AATCACGCGA 
CTGCGTAAGG TGCACAAGTG GTTGCAGCTC TTTCCCCAGC ACATCAGTGG TGAGGACCTA 
TTTGGCCTAA CGGAACCGGC CATTGTGCGA ATCCTGGAAA GCCTCCCGGG CATTGAGACG 
CTCACTGACT ACCGCTTCAA GTACGGTCGC AACCCGCTTC TTGAGTTCCC GCTAGCCATC 
AATCCGTCTG GAGCGGCGCG TACGGAACCC AAGCAACGAC AGCTGCTCGT CTGGAGGAAG 
CCGCATACGC AACGCACGGC CGGCAGCTGC AGCACGCAGC GGATGGCCAA CTCGGCTGCG 
ATTGCCGGAG AAGTGGCCTG CCCGTACAGC AAGCAGTTCG TGCATTCGAA GAGCTCCCAG 
TACAAAAAGA TGAAGCAGGA GTGGCGCAAC AACGTCTACT TGGCGAGATC TAAGATCCAG 
GGTCTTGGCT TGTATGCAGC TCGGGACATT GAAAAGCACA CCATGATAAT CGAGTACATT 
GGCGAGGTTA TCCGCACCGA GGTCTCCGAG ATACGCGAGA AGCAGTACGA GTCCAAGAAC 
CGTGGCATCT ACATGTTCCG GCTGGATGAA GACCGCGTGG TGGACGCCAC TCTAAGCGGC 
GGGTTGGCGC GCTACATCAA CCATTCGTGC AATCCCAACT GTGTTACCGA GATAGTGGAG 
GTCGATCGCG ACGTGCGGAT CATAATATTC GCCAAGCGGA AGATCTATCG CGGCGAGGAG 
CTCTCGTACG ACTACAAGTT CGACATTGAG GACGAGTCGC ACAAGATACC GTGCGCCTGT 
GGAGCGCCCA ACTGTCGCAA GTGGATGAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trr ( NM_166911.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726773.2 Drosophila melanogaster trithorax-related (trr), transcript variant D, mRNA.

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CloneID	OFa00525
Clone ID Related Accession (Same CDS sequence)	NM_166911.4
Accession Version	NM_166911.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trithorax-related, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_166911.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATAC CGAAGGTGAC AACATCGCTT GGTGCCGCCG AAAAGGCGAA ACCCGAAAGA 
GTAGCATCCG TGGCCGCCGC TGCCTTCAAT GCGGTCAGCC TGCAGAAGCG CAGCGGCGAC 
GACACCGCCA CTCCGGCGGA GGATCCCACG CGCAAGAAGG CCAAGACGGA GCTGCTTCTC 
GGAACTGGAA CGGCAGCGCC CAGTTTGCCC GCCAAAGCGT CGAGCACCGC GCCGCAGCAG 
CTCCTCTACC AGCGGAGTGG ACAGCAGGCT AAGGCGCAGG TAAAAGCTGC GTCGGAGCCG 
CAGGATGTCG AGACGGCGGA CGGCGTCTGG GACGCCCGCG ATCAGCAGAT CATAGTGTGC 
AACTTCGGCT CCGGCACTGA GATGGGTGCC ATCAAGGCGG AGGACGCTGA CAAGCAGAGT 
GAGTACAGAA TCAGCACTCC AAGGAACAGT CAATCTAACC CTTTGCTACA CCGAAACACA 
GCCTTTACCA GCTTTACCAA AAAGGAGGGT GCCTCATCGT CGGCCTCGTC ATCATCGTCA 
ACCGCGTCCG TCATTTCCAT CGAGCCCAGT GGCTCGGGGC AGGATCATGC TGAAAACTCC 
GGCAAATCTG AGGACCTCGA CTACGTACTG ATGCCAGCCA GCGGCGCGGA TAGCTCTACA 
TCTGTGGGTA ACTCCACCGG AACGGGAACA CCAGCAGGAA CGCCTATTGG AGCTACTACC 
TCCACCATTA TTTTAAACGC TAACAACGGA ACGGCAGGAG TCAGTGGAGC CGGCACCACT 
ACAATCCTCA CACAGAAATC CGGTCACACG AACTACAACA TCTTCAATAC CACGGCCACT 
GGTAGCCAAA CGCCCACTAC AACATTGTTA AACCGGGTGA ATCTTCACCC CAAGATGAAA 
ACCCAGCTGA TGGTTAACGC CAAGAAGCTG TCGGAAGTAA CGCAGACCAC GGCGAAAGTG 
TCGATTGGCA ACAAGACTAT ATCGGTGCCG CTGCTCAAGC CGCTGATGAG CGCTAGTGGA 
GCGGCCACAG CCGGAGGAGC CACGATTGTA GAGAGCAAGC AACTGCTGCA GCCGGGCGGT 
CAGGTGACTA CCGTAATGAG TGCTGCGCAG CAAAGCGGTG GTCAGCAGGT CCACCCGCAC 
GTCCATTCTC ATGCACATCA CAACTTTACC AAACTTATCA AACGCGGGCC AAAAAATAGC 
GGCACCATTG TCTCGTTTTC GGGGCTACAG ATCAAGCCGG CGAACACCAA AATTGTGGCC 
ACCAAGGTGG TGAGCAAAAA GATGCTGCAA CTTCAGCAGC ACCAACAACA GATCCAACAG 
CAGCAGCAGC TTCAGCAGCT GCAGGTGACC AGTGGTGGAG GCCTGGCTCC GCCCACAGGC 
AGCATAGTGA CTATCACCAC CACCAACCCC AGCCAAACGT ACGCAATGGT TCAGGATTCC 
GCAACTGTGG GACCTGCGGC CCATTCGGAA GACGATGCGC CAGCGCCTCG AAAGATTACA 
GCCTACTCGG AGAACCTTCA AAAAATTCTC AACAAGAGCA AGTCCCAGGA ATCGACTGGA 
GGCCCGGAGG AGTTCACCAA CATCAACAGC GTGGTGATTA AGCCGCTCGA CAAGAATACA 
CTCAATTGTC CACCCAGCTT TAACATCTTT AAGCAGCAGC AGCACTCACA GGCTGCTCAA 
AGCCAATCAA TCTCCGCGGT AGGCAGTGGA GCAGGCACAC CCGTGACGTT TACCATGGCT 
TCGGGGAATG CCTCAGATCT GGCCACCACC TCCACCGTTT CCGTTTCGGC GGGGACCATC 
TGCATAAACA GTCCGATGAT GGGCACGCGG CCAATAATCT CCATTCAGAA CAAGAACATA 
TCCCTGGTGC TGTCCAAGAC GACCATGGCC CAGCAAAAAC CGAAGATGAT AACTACAACC 
ACGTTGTCCA GTCAGGCGGC GCTGCAGATG CATCATGCTT TGATTCAGGA TTCTAGTGCA 
GACAAGGCGG GATCTTCTGC AAACTCCGGA TCAGCTACCT CGGGAGCTTC AATGCAGCTG 
AAACTAACGA CCGCCAATAC GCCCACCAAA CTCAGCGTTA GTCTGGCTCC GGACGTAGTC 
AAGCTGGAAG AAGTTGGCAG CGAATCTAAA GCAAAGCTAC TGGTCAAACA GGAGGCGGTT 
GTGAAGGATA GCACTGGTAC TCCGACCAGT GAGGAGCGGG CTGAGGAGAT CGGCACGCCG 
GAGAAGCGTT TGAACGCCAA TGCCACAATG ACGGCTATTA ACCAGGTTCA AAATCAGTCC 
GCCAATCAAA TTCAAATGGC CACATCTACC TCAACTGCCT CGAATCCCAG CACACCCAAT 
CCTACAGTGA ACGCCACACC TATGAACAAC CAACGCTCGG CAGCGGAGGA TAATGCACTT 
CTAAAGCAAC TGCTCCAAAA CAACAGCAGC AGTCATAGCC TCAACCAGAT AAGCATCACT 
TCGGCTCACG TTGGTTCCGC CTCAGCTTCG GCACCTCTTT CCGCCCGCAA GGTTATCAAC 
GTACGGGCGC CCAGCATGGG GAAGGTCAGA AGTTTGGAGG ATCAACTGGC ACGTCCGGTG 
ATTCCTCCGG TGCCCACTGC CACGCAGGCG GCAGGTAGTA GTAGTAGCAG TGGATCTGTG 
GCCACGTCCA CCACCACCAC AACAGTAGCT AGCGGAGGAA GCAGCCAACA GGTAGCCACC 
GCCTCAGCGA CGGCTCTACC TGTTAGTGCG GTCGCAATTA CCACACCAGG AGTGGGAGGG 
GAGGCAAAGC TGGAGCAGAA ATCCGATCAG CCAGCAGCAA TTATGCAGAA TCAGAGCCAA 
AACCAGGCGC CTCCACCACC ACCGCCACCA CAGCAGCAGC AGCAGCAGCA ACTACACCAG 
CCGCAGCAGC TGCAACCGTC ACCCCACCAA GTCAAGCAGA CTGTGCAGAT TGTGTCGAAG 
GAGACGTCGT TCATCTCAGG ACCCGTTGCT GCTAAAACAC TAGTGACGGA AGCCACTTCG 
AAGCCGGCTG AACTACTACC TCCTCCGCCT TACGAAATGG CCACTGCTCC TATATCGAAC 
GTCACCATAT CCATATCCAC CAAACAAGCG GCTCCGAAGG AACTGCAGAT GAAGCCCAAG 
GCGGTAGCTA TGTCACTACC CATGGAGCAG GGCGACGAAT CGCTGCCAGA GCAAGCGGAG 
CCGCCGCTTC ATTCGGAACA GGGAGCTACT GCAGCGGGAG TTGCACCTCA CTCCGGAGGT 
CCCCTTGTGT CTGCGCAATG GACCAATAAC CATTTAGAAG GAGGTGTAGC CACCACAAAG 
ATACCGTTCA AGCCTGGAGA GCCTCAAAAA CGCAAGCTCC CCATGCACCC ACAACTGGAC 
GAAAAGCAGA TCCAGCAACA GGCAGAGATT CCCATAAGCA CCTCCCTACC AACAACTCCG 
ACTGGACAAG GTACTCCCGA CAAAGTACAG CTGATCTCAG CGATTGCCAC TTATGTAAAG 
AAATCCGGTG TCCCGAACGA GGCACAACCC ATCCAGAACC AGAGCCAGGG CCAGGTGCAG 
ATGCAGGCGC AAATGCAGGC GACTATGCAA GGACACCTGT CTGGGCAGAT GTCCGGACAA 
ATCTCCGGAC ATGCCGCTGG ACAGATACCC GCTCAAATGC ATTTGCAAGT ACAGCATCAG 
CTCCATATGG CAGTTCATCC ACAGCAGCAG CAGCAGCAAC TGCACCAGAA CCAACCACAG 
AATGCGACGA TTCCCCTTCC AGTTACTGGA CAGGGTGCAG TGCCCATCCC CGTGCCCACC 
ATGGAGAGCA AGGCAGGCGA CCAAAGGAAG CGCCGCAAAC GTGAGGTCCA GAAGCCGAGG 
CGCACAAATC TGAACGCCGG ACAAGCGGGT GGAGCTCTTA AGGATCTAAC AGGTCCACTA 
CCGGCAGGTG CAATGGTCCA ACTGGCAGGA ATGCCGCCGG GGACCCAGTA CATACAAGGC 
GCGGCATCAG GGACTGGTCA TGTGATCACC AGTACCGGAC AAGGAGTTAC TTTGGGAGGA 
GTGGGCGCCA GTACTGGAGC CAGTTCCTCG CCCATGCTCA AGAAGCGGGT TCGCAAGTTC 
TCCAAGGTAG AAGAGGATCA CGATGCATTT ACCGAGAAGT TGCTGACGCA CATCCGCCAA 
ATGCAGCCGC TTCAAGTTCT GGAGCCGCAT CTCAATCGCA ACTTTCACTT TCTTATCGGG 
AGCAACGAGA CTTCTGGAGG TGGTTCGCCC GCCTCAATGA GTTCTGCGGC TTCTGCCGGA 
TCATCATCGG CTGGAGGAGG AAAGCTGAAG GGCGGATCTC GAGGCTGGCC ACTGAGCAGG 
CATTTGGAAG GTCTGGAGGA CTGTGATGGC ACCGTTCTCG GCCGCTATGG TCGAGTGAAT 
CTGCCAGGGA TACCCTCGTT GTACGATAGC GAACGCTTTG GCGGAAGTAG AGGTCTAGTT 
GGTGGATCTG CACGAACCCG TTCACCCTCG CCAGCAGAGT CTCCGGGAGC AGAAAAGATG 
CTACCAATGT CTAGCATCCA AAATGATTTT TATGACCAGG AGTTCTCCAC GCATATGGAG 
CGCAATCCTA GGGAACGCCT GGTGCGGCAC ATTGGAGCCG TCAAGGATTG CAATTTGGAG 
ACCGTAGACC TGGTGGAGAG CGAGGGCGTA GCTGCGTGGG CGACTCTTCC ACGACTTACC 
CGCTATCCGG GTCTAATACT GCTCAATGGA AACAGCCGTT GCCATGGACG GATGTCGCCG 
GTCGCACTGC CGGAGGATCC GCTGACCATG CGCTTTCCCG TGTCTCCACT GCTTCGATCC 
TGCGGCGAAG AGCTGCGCAA GACACAGCAG ATGGAGCTGG GAATGGGACC GCTGGGCAAT 
AATAATAATA ACAATTACCA GCAGAAGAAC CAGAATGTGA TACTCGCACT GCCCGCCTCG 
GCATCGGAGA ACATAGCCGG CGTGCTGCGG GATCTGGCCA ATCTACTGCA CTTGGCGCCC 
GCCCTTACCT GCAAGATCAT CGAGGACAAG ATTGGCAACA AGTTGGAGGA CCAGTTTATG 
AATCAGGACG ATGAAAAGCA CGTCGACTTC AAGCGACCAC TGAGTCAAGT GAGTCACGGT 
CATCTGAGGA AGATCCTCAA CGGGCGTCGT AAGCTCTGCC GCAGCTGCGG AAATGTGGTT 
CACGCCACTG GGCTGCGAGT TCCTCGGCAC AGTGTTCCTG CCCTAGAAGA GCAACTCCCC 
CGGTTGGCTC AACTGATGGA CATGCTGCCA AGAAAATCGG TTCCCCCTCC TTTTGTGTAC 
TTCTGCGATC GCGCCTGCTT TGCGAGATTC AAGTGGAATG GAAAGGATGG CCAGGCGGAG 
GCGGCTAGTC TGCTGCTTCA GCCAGCCGGC GGTTCTGCTG TAAAATCATC CAACGGAGAC 
TCGCCTGGCA GCTTCTGCGC CAGCTCAACT GCCCCAGCGG AAATGGTGGT CAAACAAGAA 
CCGGAGGACG AGGATGAGAA AACGCCGAGC GTACCGGGCA ACCCCACAAA TATCCCCGCA 
CAGCGCAAAT GCATTGTGAA GTGTTTTAGT GCTGATTGCT TCACCACGGA CTCGGCGCCA 
TCCGGACTGG AGCTCGACGG AACTGCTGGT GCCGGAACTG GGGCTGGACC GGTCAATAAT 
ACTGTGTGGG AAACAGAGAC CAGTGGGCTG CAGTTGGAGG ACACGCGGCA GTGTGTCTTC 
TGCAATCAGC GTGGTGACGG CCAGGCAGAT GGACCCTCGC GACTGCTTAA CTTCGATGTG 
GATAAATGGG TTCACCTTAA CTGCGCCCTG TGGTCCAACG GCGTGTACGA GACGGTGTCT 
GGGGCGCTGA TGAACTTCCA GACAGCTCTG CAAGCGGGAC TGAGCCAGGC GTGTAGCGCT 
TGTCACCAAC CAGGCGCCAC CATCAAGTGT TTCAAGTCGC GCTGCAACAG CCTGTACCAC 
CTGCCATGCG CCATTCGCGA GGAGTGCGTC TTTTACAAGA ACAAGTCCGT GCACTGCAGC 
GTTCATGGTC ATGCTCATGC CGGCATCACG ATGGGTGCAG GAGCAGGCGC CACGACTGGC 
GCCGGATTAG GAGGCTCTGT GGCAGATAAT GAACTGAGCT CCCTGGTTGT CCACCGGAGA 
GTTTTTGTCG ATCGGGATGA AAACCGTCAG GTGGCCACCG TCATGCACTA TTCAGAGCTA 
AGCAACCTGC TCCGTGTGGG CAACATGACG TTCCTGAACG TGGGTCAGTT ACTGCCGCAT 
CAGCTGGAGG CCTTCCACAC ACCCCACTAC ATCTACCCCA TTGGCTACAA GGTGAGTCGC 
TACTACTGGT GCGTGCGACG ACCCAATCGT AGGTGTCGAT ACATTTGCAG TATCGCAGAG 
GCCGGATGCA AGCCCGAGTT TCGCATCCAG GTGCAGGACG CCGGCGACAA GGAGCCGGAG 
CGCGAGTTTC GGGGCAGTTC GCCCTCGGCG GTCTGGCAGC AGATTCTGCA GCCAATCACG 
CGACTGCGTA AGGTGCACAA GTGGTTGCAG CTCTTTCCCC AGCACATCAG TGGTGAGGAC 
CTATTTGGCC TAACGGAACC GGCCATTGTG CGAATCCTGG AAAGCCTCCC GGGCATTGAG 
ACGCTCACTG ACTACCGCTT CAAGTACGGT CGCAACCCGC TTCTTGAGTT CCCGCTAGCC 
ATCAATCCGT CTGGAGCGGC GCGTACGGAA CCCAAGCAAC GACAGCTGCT CGTCTGGAGG 
AAGCCGCATA CGCAACGCAC GGCCGGCAGC TGCAGCACGC AGCGGATGGC CAACTCGGCT 
GCGATTGCCG GAGAAGTGGC CTGCCCGTAC AGCAAGCAGT TCGTGCATTC GAAGAGCTCC 
CAGTACAAAA AGATGAAGCA GGAGTGGCGC AACAACGTCT ACTTGGCGAG ATCTAAGATC 
CAGGGTCTTG GCTTGTATGC AGCTCGGGAC ATTGAAAAGC ACACCATGAT AATCGAGTAC 
ATTGGCGAGG TTATCCGCAC CGAGGTCTCC GAGATACGCG AGAAGCAGTA CGAGTCCAAG 
AACCGTGGCA TCTACATGTT CCGGCTGGAT GAAGACCGCG TGGTGGACGC CACTCTAAGC 
GGCGGGTTGG CGCGCTACAT CAACCATTCG TGCAATCCCA ACTGTGTTAC CGAGATAGTG 
GAGGTCGATC GCGACGTGCG GATCATAATA TTCGCCAAGC GGAAGATCTA TCGCGGCGAG 
GAGCTCTCGT ACGACTACAA GTTCGACATT GAGGACGAGT CGCACAAGAT ACCGTGCGCC 
TGTGGAGCGC CCAACTGTCG CAAGTGGATG AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726773.2
CDS	176..7471
Translation	MNIPKVTTSLGAAEKAKPERVASVAAAAFNAVSLQKRSGDDTATPAEDPTRKKAKTELLLGTGTAAPSLPAKASSTAPQQLLYQRSGQQAKAQVKAASEPQDVETADGVWDARDQQIIVCNFGSGTEMGAIKAEDADKQSEYRISTPRNSQSNPLLHRNTAFTSFTKKEGASSSASSSSSTASVISIEPSGSGQDHAENSGKSEDLDYVLMPASGADSSTSVGNSTGTGTPAGTPIGATTSTIILNANNGTAGVSGAGTTTILTQKSGHTNYNIFNTTATGSQTPTTTLLNRVNLHPKMKTQLMVNAKKLSEVTQTTAKVSIGNKTISVPLLKPLMSASGAATAGGATIVESKQLLQPGGQVTTVMSAAQQSGGQQVHPHVHSHAHHNFTKLIKRGPKNSGTIVSFSGLQIKPANTKIVATKVVSKKMLQLQQHQQQIQQQQQLQQLQVTSGGGLAPPTGSIVTITTTNPSQTYAMVQDSATVGPAAHSEDDAPAPRKITAYSENLQKILNKSKSQESTGGPEEFTNINSVVIKPLDKNTLNCPPSFNIFKQQQHSQAAQSQSISAVGSGAGTPVTFTMASGNASDLATTSTVSVSAGTICINSPMMGTRPIISIQNKNISLVLSKTTMAQQKPKMITTTTLSSQAALQMHHALIQDSSADKAGSSANSGSATSGASMQLKLTTANTPTKLSVSLAPDVVKLEEVGSESKAKLLVKQEAVVKDSTGTPTSEERAEEIGTPEKRLNANATMTAINQVQNQSANQIQMATSTSTASNPSTPNPTVNATPMNNQRSAAEDNALLKQLLQNNSSSHSLNQISITSAHVGSASASAPLSARKVINVRAPSMGKVRSLEDQLARPVIPPVPTATQAAGSSSSSGSVATSTTTTTVASGGSSQQVATASATALPVSAVAITTPGVGGEAKLEQKSDQPAAIMQNQSQNQAPPPPPPPQQQQQQQLHQPQQLQPSPHQVKQTVQIVSKETSFISGPVAAKTLVTEATSKPAELLPPPPYEMATAPISNVTISISTKQAAPKELQMKPKAVAMSLPMEQGDESLPEQAEPPLHSEQGATAAGVAPHSGGPLVSAQWTNNHLEGGVATTKIPFKPGEPQKRKLPMHPQLDEKQIQQQAEIPISTSLPTTPTGQGTPDKVQLISAIATYVKKSGVPNEAQPIQNQSQGQVQMQAQMQATMQGHLSGQMSGQISGHAAGQIPAQMHLQVQHQLHMAVHPQQQQQQLHQNQPQNATIPLPVTGQGAVPIPVPTMESKAGDQRKRRKREVQKPRRTNLNAGQAGGALKDLTGPLPAGAMVQLAGMPPGTQYIQGAASGTGHVITSTGQGVTLGGVGASTGASSSPMLKKRVRKFSKVEEDHDAFTEKLLTHIRQMQPLQVLEPHLNRNFHFLIGSNETSGGGSPASMSSAASAGSSSAGGGKLKGGSRGWPLSRHLEGLEDCDGTVLGRYGRVNLPGIPSLYDSERFGGSRGLVGGSARTRSPSPAESPGAEKMLPMSSIQNDFYDQEFSTHMERNPRERLVRHIGAVKDCNLETVDLVESEGVAAWATLPRLTRYPGLILLNGNSRCHGRMSPVALPEDPLTMRFPVSPLLRSCGEELRKTQQMELGMGPLGNNNNNNYQQKNQNVILALPASASENIAGVLRDLANLLHLAPALTCKIIEDKIGNKLEDQFMNQDDEKHVDFKRPLSQVSHGHLRKILNGRRKLCRSCGNVVHATGLRVPRHSVPALEEQLPRLAQLMDMLPRKSVPPPFVYFCDRACFARFKWNGKDGQAEAASLLLQPAGGSAVKSSNGDSPGSFCASSTAPAEMVVKQEPEDEDEKTPSVPGNPTNIPAQRKCIVKCFSADCFTTDSAPSGLELDGTAGAGTGAGPVNNTVWETETSGLQLEDTRQCVFCNQRGDGQADGPSRLLNFDVDKWVHLNCALWSNGVYETVSGALMNFQTALQAGLSQACSACHQPGATIKCFKSRCNSLYHLPCAIREECVFYKNKSVHCSVHGHAHAGITMGAGAGATTGAGLGGSVADNELSSLVVHRRVFVDRDENRQVATVMHYSELSNLLRVGNMTFLNVGQLLPHQLEAFHTPHYIYPIGYKVSRYYWCVRRPNRRCRYICSIAEAGCKPEFRIQVQDAGDKEPEREFRGSSPSAVWQQILQPITRLRKVHKWLQLFPQHISGEDLFGLTEPAIVRILESLPGIETLTDYRFKYGRNPLLEFPLAINPSGAARTEPKQRQLLVWRKPHTQRTAGSCSTQRMANSAAIAGEVACPYSKQFVHSKSSQYKKMKQEWRNNVYLARSKIQGLGLYAARDIEKHTMIIEYIGEVIRTEVSEIREKQYESKNRGIYMFRLDEDRVVDATLSGGLARYINHSCNPNCVTEIVEVDRDVRIIIFAKRKIYRGEELSYDYKFDIEDESHKIPCACGAPNCRKWMN

Target ORF information:

RefSeq Version	NM_166911.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trithorax-related (trr), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166911.4

ORF Insert Sequence:

ATGAATATAC CGAAGGTGAC AACATCGCTT GGTGCCGCCG AAAAGGCGAA ACCCGAAAGA 
GTAGCATCCG TGGCCGCCGC TGCCTTCAAT GCGGTCAGCC TGCAGAAGCG CAGCGGCGAC 
GACACCGCCA CTCCGGCGGA GGATCCCACG CGCAAGAAGG CCAAGACGGA GCTGCTTCTC 
GGAACTGGAA CGGCAGCGCC CAGTTTGCCC GCCAAAGCGT CGAGCACCGC GCCGCAGCAG 
CTCCTCTACC AGCGGAGTGG ACAGCAGGCT AAGGCGCAGG TAAAAGCTGC GTCGGAGCCG 
CAGGATGTCG AGACGGCGGA CGGCGTCTGG GACGCCCGCG ATCAGCAGAT CATAGTGTGC 
AACTTCGGCT CCGGCACTGA GATGGGTGCC ATCAAGGCGG AGGACGCTGA CAAGCAGAGT 
GAGTACAGAA TCAGCACTCC AAGGAACAGT CAATCTAACC CTTTGCTACA CCGAAACACA 
GCCTTTACCA GCTTTACCAA AAAGGAGGGT GCCTCATCGT CGGCCTCGTC ATCATCGTCA 
ACCGCGTCCG TCATTTCCAT CGAGCCCAGT GGCTCGGGGC AGGATCATGC TGAAAACTCC 
GGCAAATCTG AGGACCTCGA CTACGTACTG ATGCCAGCCA GCGGCGCGGA TAGCTCTACA 
TCTGTGGGTA ACTCCACCGG AACGGGAACA CCAGCAGGAA CGCCTATTGG AGCTACTACC 
TCCACCATTA TTTTAAACGC TAACAACGGA ACGGCAGGAG TCAGTGGAGC CGGCACCACT 
ACAATCCTCA CACAGAAATC CGGTCACACG AACTACAACA TCTTCAATAC CACGGCCACT 
GGTAGCCAAA CGCCCACTAC AACATTGTTA AACCGGGTGA ATCTTCACCC CAAGATGAAA 
ACCCAGCTGA TGGTTAACGC CAAGAAGCTG TCGGAAGTAA CGCAGACCAC GGCGAAAGTG 
TCGATTGGCA ACAAGACTAT ATCGGTGCCG CTGCTCAAGC CGCTGATGAG CGCTAGTGGA 
GCGGCCACAG CCGGAGGAGC CACGATTGTA GAGAGCAAGC AACTGCTGCA GCCGGGCGGT 
CAGGTGACTA CCGTAATGAG TGCTGCGCAG CAAAGCGGTG GTCAGCAGGT CCACCCGCAC 
GTCCATTCTC ATGCACATCA CAACTTTACC AAACTTATCA AACGCGGGCC AAAAAATAGC 
GGCACCATTG TCTCGTTTTC GGGGCTACAG ATCAAGCCGG CGAACACCAA AATTGTGGCC 
ACCAAGGTGG TGAGCAAAAA GATGCTGCAA CTTCAGCAGC ACCAACAACA GATCCAACAG 
CAGCAGCAGC TTCAGCAGCT GCAGGTGACC AGTGGTGGAG GCCTGGCTCC GCCCACAGGC 
AGCATAGTGA CTATCACCAC CACCAACCCC AGCCAAACGT ACGCAATGGT TCAGGATTCC 
GCAACTGTGG GACCTGCGGC CCATTCGGAA GACGATGCGC CAGCGCCTCG AAAGATTACA 
GCCTACTCGG AGAACCTTCA AAAAATTCTC AACAAGAGCA AGTCCCAGGA ATCGACTGGA 
GGCCCGGAGG AGTTCACCAA CATCAACAGC GTGGTGATTA AGCCGCTCGA CAAGAATACA 
CTCAATTGTC CACCCAGCTT TAACATCTTT AAGCAGCAGC AGCACTCACA GGCTGCTCAA 
AGCCAATCAA TCTCCGCGGT AGGCAGTGGA GCAGGCACAC CCGTGACGTT TACCATGGCT 
TCGGGGAATG CCTCAGATCT GGCCACCACC TCCACCGTTT CCGTTTCGGC GGGGACCATC 
TGCATAAACA GTCCGATGAT GGGCACGCGG CCAATAATCT CCATTCAGAA CAAGAACATA 
TCCCTGGTGC TGTCCAAGAC GACCATGGCC CAGCAAAAAC CGAAGATGAT AACTACAACC 
ACGTTGTCCA GTCAGGCGGC GCTGCAGATG CATCATGCTT TGATTCAGGA TTCTAGTGCA 
GACAAGGCGG GATCTTCTGC AAACTCCGGA TCAGCTACCT CGGGAGCTTC AATGCAGCTG 
AAACTAACGA CCGCCAATAC GCCCACCAAA CTCAGCGTTA GTCTGGCTCC GGACGTAGTC 
AAGCTGGAAG AAGTTGGCAG CGAATCTAAA GCAAAGCTAC TGGTCAAACA GGAGGCGGTT 
GTGAAGGATA GCACTGGTAC TCCGACCAGT GAGGAGCGGG CTGAGGAGAT CGGCACGCCG 
GAGAAGCGTT TGAACGCCAA TGCCACAATG ACGGCTATTA ACCAGGTTCA AAATCAGTCC 
GCCAATCAAA TTCAAATGGC CACATCTACC TCAACTGCCT CGAATCCCAG CACACCCAAT 
CCTACAGTGA ACGCCACACC TATGAACAAC CAACGCTCGG CAGCGGAGGA TAATGCACTT 
CTAAAGCAAC TGCTCCAAAA CAACAGCAGC AGTCATAGCC TCAACCAGAT AAGCATCACT 
TCGGCTCACG TTGGTTCCGC CTCAGCTTCG GCACCTCTTT CCGCCCGCAA GGTTATCAAC 
GTACGGGCGC CCAGCATGGG GAAGGTCAGA AGTTTGGAGG ATCAACTGGC ACGTCCGGTG 
ATTCCTCCGG TGCCCACTGC CACGCAGGCG GCAGGTAGTA GTAGTAGCAG TGGATCTGTG 
GCCACGTCCA CCACCACCAC AACAGTAGCT AGCGGAGGAA GCAGCCAACA GGTAGCCACC 
GCCTCAGCGA CGGCTCTACC TGTTAGTGCG GTCGCAATTA CCACACCAGG AGTGGGAGGG 
GAGGCAAAGC TGGAGCAGAA ATCCGATCAG CCAGCAGCAA TTATGCAGAA TCAGAGCCAA 
AACCAGGCGC CTCCACCACC ACCGCCACCA CAGCAGCAGC AGCAGCAGCA ACTACACCAG 
CCGCAGCAGC TGCAACCGTC ACCCCACCAA GTCAAGCAGA CTGTGCAGAT TGTGTCGAAG 
GAGACGTCGT TCATCTCAGG ACCCGTTGCT GCTAAAACAC TAGTGACGGA AGCCACTTCG 
AAGCCGGCTG AACTACTACC TCCTCCGCCT TACGAAATGG CCACTGCTCC TATATCGAAC 
GTCACCATAT CCATATCCAC CAAACAAGCG GCTCCGAAGG AACTGCAGAT GAAGCCCAAG 
GCGGTAGCTA TGTCACTACC CATGGAGCAG GGCGACGAAT CGCTGCCAGA GCAAGCGGAG 
CCGCCGCTTC ATTCGGAACA GGGAGCTACT GCAGCGGGAG TTGCACCTCA CTCCGGAGGT 
CCCCTTGTGT CTGCGCAATG GACCAATAAC CATTTAGAAG GAGGTGTAGC CACCACAAAG 
ATACCGTTCA AGCCTGGAGA GCCTCAAAAA CGCAAGCTCC CCATGCACCC ACAACTGGAC 
GAAAAGCAGA TCCAGCAACA GGCAGAGATT CCCATAAGCA CCTCCCTACC AACAACTCCG 
ACTGGACAAG GTACTCCCGA CAAAGTACAG CTGATCTCAG CGATTGCCAC TTATGTAAAG 
AAATCCGGTG TCCCGAACGA GGCACAACCC ATCCAGAACC AGAGCCAGGG CCAGGTGCAG 
ATGCAGGCGC AAATGCAGGC GACTATGCAA GGACACCTGT CTGGGCAGAT GTCCGGACAA 
ATCTCCGGAC ATGCCGCTGG ACAGATACCC GCTCAAATGC ATTTGCAAGT ACAGCATCAG 
CTCCATATGG CAGTTCATCC ACAGCAGCAG CAGCAGCAAC TGCACCAGAA CCAACCACAG 
AATGCGACGA TTCCCCTTCC AGTTACTGGA CAGGGTGCAG TGCCCATCCC CGTGCCCACC 
ATGGAGAGCA AGGCAGGCGA CCAAAGGAAG CGCCGCAAAC GTGAGGTCCA GAAGCCGAGG 
CGCACAAATC TGAACGCCGG ACAAGCGGGT GGAGCTCTTA AGGATCTAAC AGGTCCACTA 
CCGGCAGGTG CAATGGTCCA ACTGGCAGGA ATGCCGCCGG GGACCCAGTA CATACAAGGC 
GCGGCATCAG GGACTGGTCA TGTGATCACC AGTACCGGAC AAGGAGTTAC TTTGGGAGGA 
GTGGGCGCCA GTACTGGAGC CAGTTCCTCG CCCATGCTCA AGAAGCGGGT TCGCAAGTTC 
TCCAAGGTAG AAGAGGATCA CGATGCATTT ACCGAGAAGT TGCTGACGCA CATCCGCCAA 
ATGCAGCCGC TTCAAGTTCT GGAGCCGCAT CTCAATCGCA ACTTTCACTT TCTTATCGGG 
AGCAACGAGA CTTCTGGAGG TGGTTCGCCC GCCTCAATGA GTTCTGCGGC TTCTGCCGGA 
TCATCATCGG CTGGAGGAGG AAAGCTGAAG GGCGGATCTC GAGGCTGGCC ACTGAGCAGG 
CATTTGGAAG GTCTGGAGGA CTGTGATGGC ACCGTTCTCG GCCGCTATGG TCGAGTGAAT 
CTGCCAGGGA TACCCTCGTT GTACGATAGC GAACGCTTTG GCGGAAGTAG AGGTCTAGTT 
GGTGGATCTG CACGAACCCG TTCACCCTCG CCAGCAGAGT CTCCGGGAGC AGAAAAGATG 
CTACCAATGT CTAGCATCCA AAATGATTTT TATGACCAGG AGTTCTCCAC GCATATGGAG 
CGCAATCCTA GGGAACGCCT GGTGCGGCAC ATTGGAGCCG TCAAGGATTG CAATTTGGAG 
ACCGTAGACC TGGTGGAGAG CGAGGGCGTA GCTGCGTGGG CGACTCTTCC ACGACTTACC 
CGCTATCCGG GTCTAATACT GCTCAATGGA AACAGCCGTT GCCATGGACG GATGTCGCCG 
GTCGCACTGC CGGAGGATCC GCTGACCATG CGCTTTCCCG TGTCTCCACT GCTTCGATCC 
TGCGGCGAAG AGCTGCGCAA GACACAGCAG ATGGAGCTGG GAATGGGACC GCTGGGCAAT 
AATAATAATA ACAATTACCA GCAGAAGAAC CAGAATGTGA TACTCGCACT GCCCGCCTCG 
GCATCGGAGA ACATAGCCGG CGTGCTGCGG GATCTGGCCA ATCTACTGCA CTTGGCGCCC 
GCCCTTACCT GCAAGATCAT CGAGGACAAG ATTGGCAACA AGTTGGAGGA CCAGTTTATG 
AATCAGGACG ATGAAAAGCA CGTCGACTTC AAGCGACCAC TGAGTCAAGT GAGTCACGGT 
CATCTGAGGA AGATCCTCAA CGGGCGTCGT AAGCTCTGCC GCAGCTGCGG AAATGTGGTT 
CACGCCACTG GGCTGCGAGT TCCTCGGCAC AGTGTTCCTG CCCTAGAAGA GCAACTCCCC 
CGGTTGGCTC AACTGATGGA CATGCTGCCA AGAAAATCGG TTCCCCCTCC TTTTGTGTAC 
TTCTGCGATC GCGCCTGCTT TGCGAGATTC AAGTGGAATG GAAAGGATGG CCAGGCGGAG 
GCGGCTAGTC TGCTGCTTCA GCCAGCCGGC GGTTCTGCTG TAAAATCATC CAACGGAGAC 
TCGCCTGGCA GCTTCTGCGC CAGCTCAACT GCCCCAGCGG AAATGGTGGT CAAACAAGAA 
CCGGAGGACG AGGATGAGAA AACGCCGAGC GTACCGGGCA ACCCCACAAA TATCCCCGCA 
CAGCGCAAAT GCATTGTGAA GTGTTTTAGT GCTGATTGCT TCACCACGGA CTCGGCGCCA 
TCCGGACTGG AGCTCGACGG AACTGCTGGT GCCGGAACTG GGGCTGGACC GGTCAATAAT 
ACTGTGTGGG AAACAGAGAC CAGTGGGCTG CAGTTGGAGG ACACGCGGCA GTGTGTCTTC 
TGCAATCAGC GTGGTGACGG CCAGGCAGAT GGACCCTCGC GACTGCTTAA CTTCGATGTG 
GATAAATGGG TTCACCTTAA CTGCGCCCTG TGGTCCAACG GCGTGTACGA GACGGTGTCT 
GGGGCGCTGA TGAACTTCCA GACAGCTCTG CAAGCGGGAC TGAGCCAGGC GTGTAGCGCT 
TGTCACCAAC CAGGCGCCAC CATCAAGTGT TTCAAGTCGC GCTGCAACAG CCTGTACCAC 
CTGCCATGCG CCATTCGCGA GGAGTGCGTC TTTTACAAGA ACAAGTCCGT GCACTGCAGC 
GTTCATGGTC ATGCTCATGC CGGCATCACG ATGGGTGCAG GAGCAGGCGC CACGACTGGC 
GCCGGATTAG GAGGCTCTGT GGCAGATAAT GAACTGAGCT CCCTGGTTGT CCACCGGAGA 
GTTTTTGTCG ATCGGGATGA AAACCGTCAG GTGGCCACCG TCATGCACTA TTCAGAGCTA 
AGCAACCTGC TCCGTGTGGG CAACATGACG TTCCTGAACG TGGGTCAGTT ACTGCCGCAT 
CAGCTGGAGG CCTTCCACAC ACCCCACTAC ATCTACCCCA TTGGCTACAA GGTGAGTCGC 
TACTACTGGT GCGTGCGACG ACCCAATCGT AGGTGTCGAT ACATTTGCAG TATCGCAGAG 
GCCGGATGCA AGCCCGAGTT TCGCATCCAG GTGCAGGACG CCGGCGACAA GGAGCCGGAG 
CGCGAGTTTC GGGGCAGTTC GCCCTCGGCG GTCTGGCAGC AGATTCTGCA GCCAATCACG 
CGACTGCGTA AGGTGCACAA GTGGTTGCAG CTCTTTCCCC AGCACATCAG TGGTGAGGAC 
CTATTTGGCC TAACGGAACC GGCCATTGTG CGAATCCTGG AAAGCCTCCC GGGCATTGAG 
ACGCTCACTG ACTACCGCTT CAAGTACGGT CGCAACCCGC TTCTTGAGTT CCCGCTAGCC 
ATCAATCCGT CTGGAGCGGC GCGTACGGAA CCCAAGCAAC GACAGCTGCT CGTCTGGAGG 
AAGCCGCATA CGCAACGCAC GGCCGGCAGC TGCAGCACGC AGCGGATGGC CAACTCGGCT 
GCGATTGCCG GAGAAGTGGC CTGCCCGTAC AGCAAGCAGT TCGTGCATTC GAAGAGCTCC 
CAGTACAAAA AGATGAAGCA GGAGTGGCGC AACAACGTCT ACTTGGCGAG ATCTAAGATC 
CAGGGTCTTG GCTTGTATGC AGCTCGGGAC ATTGAAAAGC ACACCATGAT AATCGAGTAC 
ATTGGCGAGG TTATCCGCAC CGAGGTCTCC GAGATACGCG AGAAGCAGTA CGAGTCCAAG 
AACCGTGGCA TCTACATGTT CCGGCTGGAT GAAGACCGCG TGGTGGACGC CACTCTAAGC 
GGCGGGTTGG CGCGCTACAT CAACCATTCG TGCAATCCCA ACTGTGTTAC CGAGATAGTG 
GAGGTCGATC GCGACGTGCG GATCATAATA TTCGCCAAGC GGAAGATCTA TCGCGGCGAG 
GAGCTCTCGT ACGACTACAA GTTCGACATT GAGGACGAGT CGCACAAGAT ACCGTGCGCC 
TGTGGAGCGC CCAACTGTCG CAAGTGGATG AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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trr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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