tw cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tw
Entrez Gene ID	31024
Full Name	twisted
Synonyms	CG12311,DPOMT2,DmPOMT2,Dmel\CG12311,EG:34F3.7,POMT2,Pomt2,Tw,dPOMT2,dPomt2
General protein information	Preferred Names twisted Names CG12311 gene product from transcript CG12311-RB CG12311-PA CG12311-PB protein O-mannosyltransferase 2 tricky-dickey tw-PA tw-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 1C4-1C4\|1-0.4 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_130502.3	NP_569858.1	twisted, isoform A
NM_001272173.1	NP_001259102.1	twisted, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	POMT2	XP_510091.2
Mus musculus (house mouse)	Pomt2	NP_700464.2
Magnaporthe oryzae (rice blast fungus)	MGG_07190	XP_003715396.1
Homo sapiens (human)	POMT2	NP_037514.2
	POMT2	XP_001101522.1
Rattus norvegicus (Norway rat)	Pomt2	NP_001040579.1
Gallus gallus (chicken)	POMT2	XP_004941879.1
Eremothecium gossypii	AGOS_ACR290W	NP_983692.1
Neurospora crassa	NCU01648	XP_956270.2
Xenopus tropicalis (tropical clawed frog)	pomt2	XP_004917112.1
Danio rerio (zebrafish)	pomt2	NP_001038498.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002674	XP_312249.5
Saccharomyces cerevisiae (baker's yeast)	PMT2	NP_009379.2
Schizosaccharomyces pombe (fission yeast)	ogm2	NP_594135.1
Canis lupus familiaris (dog)	POMT2	XP_537521.4
Bos taurus (cattle)	POMT2	NP_001179825.1
Drosophila melanogaster (fruit fly)	tw	NP_569858.1
Kluyveromyces lactis	KLLA0F15829g	XP_455792.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study demonstrated that expression of RNA interference (RNAi) for the rt gene and the tw mutant was almost completely lethal and semi-lethal, respectively.
Title: Increased apoptosis of myoblasts in Drosophila model for the Walker-Warburg syndrome.

establish a central role for Dystroglycan (Dg) in maintaining integrity in Drosophila larval muscles and demonstrate the importance of glycosylation to Dg function in Drosophila
Title: Dystroglycan and protein O-mannosyltransferases 1 and 2 are required to maintain integrity of Drosophila larval muscles.

Drosophila POMT1 and POMT2 function as a protein O-mannosyltransferase in association with each other to generate and maintain normal muscle development.
Title: The twisted abdomen phenotype of Drosophila POMT1 and POMT2 mutants coincides with their heterophilic protein O-mannosyltransferase activity.

molecular cloning and characterization of POMT2
Title: Characterization of POMT2, a novel member of the PMT protein O-mannosyltransferase family specifically localized to the acrosome of mammalian spermatids.

The following tw gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tw cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00402	NM_130502.3 Latest version!	Drosophila melanogaster twisted (tw), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00402	NM_001272173.1 Latest version!	Drosophila melanogaster twisted (tw), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tw ( NM_130502.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569858.1 Drosophila melanogaster twisted (tw), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00402
Clone ID Related Accession (Same CDS sequence)	NM_130502.3 , NM_001272173.1
Accession Version	NM_130502.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twisted, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_130502.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAA GTGTTGTTAA AACCCCCAAG TGCCCCAGAC GAGGATCTGT CAAAGATGTG 
GCCCAAAACG CACCTAGAAC AGCACCCACA TCTAGCAAAG AGGCCAATTG GAACTGGTGG 
CTTCTCCTGG CTACCGTCTT CCTGGTGACC TTCGCCACCC GCTTCTATAA GGTCACCGAG 
CCGGATCACA TATGTTGGGA TGAGACGCAC TTTGGCAAGA TGGGCAGTTG GTACATTAAC 
AGAACCTTTT TTTTCGATGT CCATCCACCG CTAGGAAAGA TGCTCATTGG CCTCTCTGGC 
TACTTGACGG GCTACAATGG AACGTTTCCG TTTGAGAAGC CAGGTGACAA GTACAACGAG 
ACGCGGTACC AGGGCATGCG ATATTTCTGC ACCACACTGG GAGCTCTGAT CATGCCCATG 
GGATTCGATA CTGTCTACGA TTTGACCCGT TCCCATGAAG CTGCCTTGCT TGCGGCTGCC 
TATCTTATTT TCGATGTGGG GCTCTTGACC CTGAACCAGT ACATCCTGCT GGACCCCATT 
CTGTTATTCT TCATGATGGC CTCCGTCTGG GGCATGGTCA AGGTATCGAA GTCCACGGCC 
TCCGGAGGAT CGTACGGTCT ACGCTGGTGG CTCTGGCTCT TCCTCACCGG CACCATGTTG 
TCCTGCACGA TTAGCGTTAA GTTCGTGGGT CTGTTTGTGG TGCTGCTGGT CGGTCTGCAT 
ACAGCCACTG AACTGTGGCT CATCCTCGGC GATTTGGGGC AGCCCATTTT GGAGACTGTT 
AAGCAGTTGG CCTGCCGGGC CATCACCCTG ATTGTCTGGC CCGTTCTCCT CTACATCCTG 
TTCTTCTACA TTCACCTGAG TGTCCTTAAC CGCAGCGGCA ACGGAGATGG GTTCTATAGT 
TCGGCCTTTC AGTCCAGGCT GATCGGCAAC TCCCTATATA ATGCCAGCAT GCCCAGAGAT 
GTCGCCTACG GATCGCTAGT GACAATCAAG AACCACAAGA CGGGCGGAGG CTACCTGCAC 
TCGCATCATC ACCTGTATCC CAAGGGATCT GGCGCTAGGC AGCAGCAGGT TACCACTTAC 
ACCCACAAGG ACGAGAACAA CAAGTGGCTA ATCAGGCCGC ACAACAAGCC GGGTCCACCA 
AAGGGCAAGG TACAGATCCT CCGGCACGGG GACCTCGTCC GCCTGACTCA CATGGCGACC 
AGGAGAAATC TGCATTCCCA CAACGAACCT GCACCCATGA CCAAGAAGCA TTTGCAGGTC 
ACAGGCTATG GCGAGTTGGG ACTAGGCGAT GCCAACGACG TCTGGCGCGT GTTGATTGTG 
GGCGGCAAGG TCAACGAAAC GGTACACACG GTCACTTCCC GTCTCAAGTT CATCCACCTG 
CTGCAGAACT GCGCCCTGAC CTCCAGTGGG AAGCAGCTAC CGAAATGGGG GTTCGAACAG 
CAGGAGGTGT CATGCAATCC CAACGTGCGC GACAAGAACT CGCAGTGGAA CGTCGAGGAC 
AACGAGCACA AGTTGATGCC CAGTGTAAGC TTTAGCGTGT ATGCGCCCGG CTTCTTTGCC 
CGCTTCCTCG AATCCCACGC CGTAATGCTG CAGGGAAACG CAGGACTCAA GCCCAAAGAA 
GGCGAGGTCA CCAGCAGACC CTGGCAGTGG CCAATCAACT ACAGGGGCCA GTTCTTCTCC 
GGCAGCAGTT ACCGCATCTA CTTGCTGGGA AACCCGTTGA TTTGGTGGAG TAATCTGGTT 
TTCCTGGCCC TTTTCGTCAC GGTTTTCCTG TGCAACGCAG TTGTTCAGCA AAGAAGAGCT 
GGCTTCGCCC GATCGGCGGC CCAAAATCAA GCCCAAGTTC CCGATTCGGA AACAGTGGCC 
CAGGACGAGG AGAGTGAGCA CTCCACCACG GACATCTGCA GTTGTTGTAC TCCGGCGAAG 
GAGATAGTCC CAAAGGCAGT TCCCAGTGGG TCCCCGGAGG CACCCAACCC GGCGCAGTCC 
TTAAGGGCGG CCGCCTGGCT TTTTCTGGGC TGGATGCTCC ACTATTTGCC CTTCTGGGCG 
ATGGGACGGG TGCTCTACTT CCACCACTAT TTTCCGGCCT TGATCTTCAA CTCCTTGCTG 
ACGGGCGTTA TGTACAACTA CATTTTGAGG GTTCTGCCCA AATGGATTCA CCACGTTATC 
CTCGGACTGG TTCTCTCGAT CCTGGTGTAT AGTTTTGCCG CCTTCTCGCC ACTTGCTTAC 
GGAATGAGTG GTCCTCTAGC CAACGAGCCG AACTCTACAA TGTACAATCT CAAGTGGCTT 
TCTACCTGGG AGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569858.1
CDS	160..2457
Translation	MAASVVKTPKCPRRGSVKDVAQNAPRTAPTSSKEANWNWWLLLATVFLVTFATRFYKVTEPDHICWDETHFGKMGSWYINRTFFFDVHPPLGKMLIGLSGYLTGYNGTFPFEKPGDKYNETRYQGMRYFCTTLGALIMPMGFDTVYDLTRSHEAALLAAAYLIFDVGLLTLNQYILLDPILLFFMMASVWGMVKVSKSTASGGSYGLRWWLWLFLTGTMLSCTISVKFVGLFVVLLVGLHTATELWLILGDLGQPILETVKQLACRAITLIVWPVLLYILFFYIHLSVLNRSGNGDGFYSSAFQSRLIGNSLYNASMPRDVAYGSLVTIKNHKTGGGYLHSHHHLYPKGSGARQQQVTTYTHKDENNKWLIRPHNKPGPPKGKVQILRHGDLVRLTHMATRRNLHSHNEPAPMTKKHLQVTGYGELGLGDANDVWRVLIVGGKVNETVHTVTSRLKFIHLLQNCALTSSGKQLPKWGFEQQEVSCNPNVRDKNSQWNVEDNEHKLMPSVSFSVYAPGFFARFLESHAVMLQGNAGLKPKEGEVTSRPWQWPINYRGQFFSGSSYRIYLLGNPLIWWSNLVFLALFVTVFLCNAVVQQRRAGFARSAAQNQAQVPDSETVAQDEESEHSTTDICSCCTPAKEIVPKAVPSGSPEAPNPAQSLRAAAWLFLGWMLHYLPFWAMGRVLYFHHYFPALIFNSLLTGVMYNYILRVLPKWIHHVILGLVLSILVYSFAAFSPLAYGMSGPLANEPNSTMYNLKWLSTWEF

Target ORF information:

RefSeq Version	NM_130502.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twisted (tw), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130502.3

ORF Insert Sequence:

ATGGCAGCAA GTGTTGTTAA AACCCCCAAG TGCCCCAGAC GAGGATCTGT CAAAGATGTG 
GCCCAAAACG CACCTAGAAC AGCACCCACA TCTAGCAAAG AGGCCAATTG GAACTGGTGG 
CTTCTCCTGG CTACCGTCTT CCTGGTGACC TTCGCCACCC GCTTCTATAA GGTCACCGAG 
CCGGATCACA TATGTTGGGA TGAGACGCAC TTTGGCAAGA TGGGCAGTTG GTACATTAAC 
AGAACCTTTT TTTTCGATGT CCATCCACCG CTAGGAAAGA TGCTCATTGG CCTCTCTGGC 
TACTTGACGG GCTACAATGG AACGTTTCCG TTTGAGAAGC CAGGTGACAA GTACAACGAG 
ACGCGGTACC AGGGCATGCG ATATTTCTGC ACCACACTGG GAGCTCTGAT CATGCCCATG 
GGATTCGATA CTGTCTACGA TTTGACCCGT TCCCATGAAG CTGCCTTGCT TGCGGCTGCC 
TATCTTATTT TCGATGTGGG GCTCTTGACC CTGAACCAGT ACATCCTGCT GGACCCCATT 
CTGTTATTCT TCATGATGGC CTCCGTCTGG GGCATGGTCA AGGTATCGAA GTCCACGGCC 
TCCGGAGGAT CGTACGGTCT ACGCTGGTGG CTCTGGCTCT TCCTCACCGG CACCATGTTG 
TCCTGCACGA TTAGCGTTAA GTTCGTGGGT CTGTTTGTGG TGCTGCTGGT CGGTCTGCAT 
ACAGCCACTG AACTGTGGCT CATCCTCGGC GATTTGGGGC AGCCCATTTT GGAGACTGTT 
AAGCAGTTGG CCTGCCGGGC CATCACCCTG ATTGTCTGGC CCGTTCTCCT CTACATCCTG 
TTCTTCTACA TTCACCTGAG TGTCCTTAAC CGCAGCGGCA ACGGAGATGG GTTCTATAGT 
TCGGCCTTTC AGTCCAGGCT GATCGGCAAC TCCCTATATA ATGCCAGCAT GCCCAGAGAT 
GTCGCCTACG GATCGCTAGT GACAATCAAG AACCACAAGA CGGGCGGAGG CTACCTGCAC 
TCGCATCATC ACCTGTATCC CAAGGGATCT GGCGCTAGGC AGCAGCAGGT TACCACTTAC 
ACCCACAAGG ACGAGAACAA CAAGTGGCTA ATCAGGCCGC ACAACAAGCC GGGTCCACCA 
AAGGGCAAGG TACAGATCCT CCGGCACGGG GACCTCGTCC GCCTGACTCA CATGGCGACC 
AGGAGAAATC TGCATTCCCA CAACGAACCT GCACCCATGA CCAAGAAGCA TTTGCAGGTC 
ACAGGCTATG GCGAGTTGGG ACTAGGCGAT GCCAACGACG TCTGGCGCGT GTTGATTGTG 
GGCGGCAAGG TCAACGAAAC GGTACACACG GTCACTTCCC GTCTCAAGTT CATCCACCTG 
CTGCAGAACT GCGCCCTGAC CTCCAGTGGG AAGCAGCTAC CGAAATGGGG GTTCGAACAG 
CAGGAGGTGT CATGCAATCC CAACGTGCGC GACAAGAACT CGCAGTGGAA CGTCGAGGAC 
AACGAGCACA AGTTGATGCC CAGTGTAAGC TTTAGCGTGT ATGCGCCCGG CTTCTTTGCC 
CGCTTCCTCG AATCCCACGC CGTAATGCTG CAGGGAAACG CAGGACTCAA GCCCAAAGAA 
GGCGAGGTCA CCAGCAGACC CTGGCAGTGG CCAATCAACT ACAGGGGCCA GTTCTTCTCC 
GGCAGCAGTT ACCGCATCTA CTTGCTGGGA AACCCGTTGA TTTGGTGGAG TAATCTGGTT 
TTCCTGGCCC TTTTCGTCAC GGTTTTCCTG TGCAACGCAG TTGTTCAGCA AAGAAGAGCT 
GGCTTCGCCC GATCGGCGGC CCAAAATCAA GCCCAAGTTC CCGATTCGGA AACAGTGGCC 
CAGGACGAGG AGAGTGAGCA CTCCACCACG GACATCTGCA GTTGTTGTAC TCCGGCGAAG 
GAGATAGTCC CAAAGGCAGT TCCCAGTGGG TCCCCGGAGG CACCCAACCC GGCGCAGTCC 
TTAAGGGCGG CCGCCTGGCT TTTTCTGGGC TGGATGCTCC ACTATTTGCC CTTCTGGGCG 
ATGGGACGGG TGCTCTACTT CCACCACTAT TTTCCGGCCT TGATCTTCAA CTCCTTGCTG 
ACGGGCGTTA TGTACAACTA CATTTTGAGG GTTCTGCCCA AATGGATTCA CCACGTTATC 
CTCGGACTGG TTCTCTCGAT CCTGGTGTAT AGTTTTGCCG CCTTCTCGCC ACTTGCTTAC 
GGAATGAGTG GTCCTCTAGC CAACGAGCCG AACTCTACAA TGTACAATCT CAAGTGGCTT 
TCTACCTGGG AGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tw ( NM_001272173.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259102.1 Drosophila melanogaster twisted (tw), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00402
Clone ID Related Accession (Same CDS sequence)	NM_130502.3 , NM_001272173.1
Accession Version	NM_001272173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twisted, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCAA GTGTTGTTAA AACCCCCAAG TGCCCCAGAC GAGGATCTGT CAAAGATGTG 
GCCCAAAACG CACCTAGAAC AGCACCCACA TCTAGCAAAG AGGCCAATTG GAACTGGTGG 
CTTCTCCTGG CTACCGTCTT CCTGGTGACC TTCGCCACCC GCTTCTATAA GGTCACCGAG 
CCGGATCACA TATGTTGGGA TGAGACGCAC TTTGGCAAGA TGGGCAGTTG GTACATTAAC 
AGAACCTTTT TTTTCGATGT CCATCCACCG CTAGGAAAGA TGCTCATTGG CCTCTCTGGC 
TACTTGACGG GCTACAATGG AACGTTTCCG TTTGAGAAGC CAGGTGACAA GTACAACGAG 
ACGCGGTACC AGGGCATGCG ATATTTCTGC ACCACACTGG GAGCTCTGAT CATGCCCATG 
GGATTCGATA CTGTCTACGA TTTGACCCGT TCCCATGAAG CTGCCTTGCT TGCGGCTGCC 
TATCTTATTT TCGATGTGGG GCTCTTGACC CTGAACCAGT ACATCCTGCT GGACCCCATT 
CTGTTATTCT TCATGATGGC CTCCGTCTGG GGCATGGTCA AGGTATCGAA GTCCACGGCC 
TCCGGAGGAT CGTACGGTCT ACGCTGGTGG CTCTGGCTCT TCCTCACCGG CACCATGTTG 
TCCTGCACGA TTAGCGTTAA GTTCGTGGGT CTGTTTGTGG TGCTGCTGGT CGGTCTGCAT 
ACAGCCACTG AACTGTGGCT CATCCTCGGC GATTTGGGGC AGCCCATTTT GGAGACTGTT 
AAGCAGTTGG CCTGCCGGGC CATCACCCTG ATTGTCTGGC CCGTTCTCCT CTACATCCTG 
TTCTTCTACA TTCACCTGAG TGTCCTTAAC CGCAGCGGCA ACGGAGATGG GTTCTATAGT 
TCGGCCTTTC AGTCCAGGCT GATCGGCAAC TCCCTATATA ATGCCAGCAT GCCCAGAGAT 
GTCGCCTACG GATCGCTAGT GACAATCAAG AACCACAAGA CGGGCGGAGG CTACCTGCAC 
TCGCATCATC ACCTGTATCC CAAGGGATCT GGCGCTAGGC AGCAGCAGGT TACCACTTAC 
ACCCACAAGG ACGAGAACAA CAAGTGGCTA ATCAGGCCGC ACAACAAGCC GGGTCCACCA 
AAGGGCAAGG TACAGATCCT CCGGCACGGG GACCTCGTCC GCCTGACTCA CATGGCGACC 
AGGAGAAATC TGCATTCCCA CAACGAACCT GCACCCATGA CCAAGAAGCA TTTGCAGGTC 
ACAGGCTATG GCGAGTTGGG ACTAGGCGAT GCCAACGACG TCTGGCGCGT GTTGATTGTG 
GGCGGCAAGG TCAACGAAAC GGTACACACG GTCACTTCCC GTCTCAAGTT CATCCACCTG 
CTGCAGAACT GCGCCCTGAC CTCCAGTGGG AAGCAGCTAC CGAAATGGGG GTTCGAACAG 
CAGGAGGTGT CATGCAATCC CAACGTGCGC GACAAGAACT CGCAGTGGAA CGTCGAGGAC 
AACGAGCACA AGTTGATGCC CAGTGTAAGC TTTAGCGTGT ATGCGCCCGG CTTCTTTGCC 
CGCTTCCTCG AATCCCACGC CGTAATGCTG CAGGGAAACG CAGGACTCAA GCCCAAAGAA 
GGCGAGGTCA CCAGCAGACC CTGGCAGTGG CCAATCAACT ACAGGGGCCA GTTCTTCTCC 
GGCAGCAGTT ACCGCATCTA CTTGCTGGGA AACCCGTTGA TTTGGTGGAG TAATCTGGTT 
TTCCTGGCCC TTTTCGTCAC GGTTTTCCTG TGCAACGCAG TTGTTCAGCA AAGAAGAGCT 
GGCTTCGCCC GATCGGCGGC CCAAAATCAA GCCCAAGTTC CCGATTCGGA AACAGTGGCC 
CAGGACGAGG AGAGTGAGCA CTCCACCACG GACATCTGCA GTTGTTGTAC TCCGGCGAAG 
GAGATAGTCC CAAAGGCAGT TCCCAGTGGG TCCCCGGAGG CACCCAACCC GGCGCAGTCC 
TTAAGGGCGG CCGCCTGGCT TTTTCTGGGC TGGATGCTCC ACTATTTGCC CTTCTGGGCG 
ATGGGACGGG TGCTCTACTT CCACCACTAT TTTCCGGCCT TGATCTTCAA CTCCTTGCTG 
ACGGGCGTTA TGTACAACTA CATTTTGAGG GTTCTGCCCA AATGGATTCA CCACGTTATC 
CTCGGACTGG TTCTCTCGAT CCTGGTGTAT AGTTTTGCCG CCTTCTCGCC ACTTGCTTAC 
GGAATGAGTG GTCCTCTAGC CAACGAGCCG AACTCTACAA TGTACAATCT CAAGTGGCTT 
TCTACCTGGG AGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259102.1
CDS	160..2457
Translation	MAASVVKTPKCPRRGSVKDVAQNAPRTAPTSSKEANWNWWLLLATVFLVTFATRFYKVTEPDHICWDETHFGKMGSWYINRTFFFDVHPPLGKMLIGLSGYLTGYNGTFPFEKPGDKYNETRYQGMRYFCTTLGALIMPMGFDTVYDLTRSHEAALLAAAYLIFDVGLLTLNQYILLDPILLFFMMASVWGMVKVSKSTASGGSYGLRWWLWLFLTGTMLSCTISVKFVGLFVVLLVGLHTATELWLILGDLGQPILETVKQLACRAITLIVWPVLLYILFFYIHLSVLNRSGNGDGFYSSAFQSRLIGNSLYNASMPRDVAYGSLVTIKNHKTGGGYLHSHHHLYPKGSGARQQQVTTYTHKDENNKWLIRPHNKPGPPKGKVQILRHGDLVRLTHMATRRNLHSHNEPAPMTKKHLQVTGYGELGLGDANDVWRVLIVGGKVNETVHTVTSRLKFIHLLQNCALTSSGKQLPKWGFEQQEVSCNPNVRDKNSQWNVEDNEHKLMPSVSFSVYAPGFFARFLESHAVMLQGNAGLKPKEGEVTSRPWQWPINYRGQFFSGSSYRIYLLGNPLIWWSNLVFLALFVTVFLCNAVVQQRRAGFARSAAQNQAQVPDSETVAQDEESEHSTTDICSCCTPAKEIVPKAVPSGSPEAPNPAQSLRAAAWLFLGWMLHYLPFWAMGRVLYFHHYFPALIFNSLLTGVMYNYILRVLPKWIHHVILGLVLSILVYSFAAFSPLAYGMSGPLANEPNSTMYNLKWLSTWEF

Target ORF information:

RefSeq Version	NM_001272173.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twisted (tw), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272173.1

ORF Insert Sequence:

ATGGCAGCAA GTGTTGTTAA AACCCCCAAG TGCCCCAGAC GAGGATCTGT CAAAGATGTG 
GCCCAAAACG CACCTAGAAC AGCACCCACA TCTAGCAAAG AGGCCAATTG GAACTGGTGG 
CTTCTCCTGG CTACCGTCTT CCTGGTGACC TTCGCCACCC GCTTCTATAA GGTCACCGAG 
CCGGATCACA TATGTTGGGA TGAGACGCAC TTTGGCAAGA TGGGCAGTTG GTACATTAAC 
AGAACCTTTT TTTTCGATGT CCATCCACCG CTAGGAAAGA TGCTCATTGG CCTCTCTGGC 
TACTTGACGG GCTACAATGG AACGTTTCCG TTTGAGAAGC CAGGTGACAA GTACAACGAG 
ACGCGGTACC AGGGCATGCG ATATTTCTGC ACCACACTGG GAGCTCTGAT CATGCCCATG 
GGATTCGATA CTGTCTACGA TTTGACCCGT TCCCATGAAG CTGCCTTGCT TGCGGCTGCC 
TATCTTATTT TCGATGTGGG GCTCTTGACC CTGAACCAGT ACATCCTGCT GGACCCCATT 
CTGTTATTCT TCATGATGGC CTCCGTCTGG GGCATGGTCA AGGTATCGAA GTCCACGGCC 
TCCGGAGGAT CGTACGGTCT ACGCTGGTGG CTCTGGCTCT TCCTCACCGG CACCATGTTG 
TCCTGCACGA TTAGCGTTAA GTTCGTGGGT CTGTTTGTGG TGCTGCTGGT CGGTCTGCAT 
ACAGCCACTG AACTGTGGCT CATCCTCGGC GATTTGGGGC AGCCCATTTT GGAGACTGTT 
AAGCAGTTGG CCTGCCGGGC CATCACCCTG ATTGTCTGGC CCGTTCTCCT CTACATCCTG 
TTCTTCTACA TTCACCTGAG TGTCCTTAAC CGCAGCGGCA ACGGAGATGG GTTCTATAGT 
TCGGCCTTTC AGTCCAGGCT GATCGGCAAC TCCCTATATA ATGCCAGCAT GCCCAGAGAT 
GTCGCCTACG GATCGCTAGT GACAATCAAG AACCACAAGA CGGGCGGAGG CTACCTGCAC 
TCGCATCATC ACCTGTATCC CAAGGGATCT GGCGCTAGGC AGCAGCAGGT TACCACTTAC 
ACCCACAAGG ACGAGAACAA CAAGTGGCTA ATCAGGCCGC ACAACAAGCC GGGTCCACCA 
AAGGGCAAGG TACAGATCCT CCGGCACGGG GACCTCGTCC GCCTGACTCA CATGGCGACC 
AGGAGAAATC TGCATTCCCA CAACGAACCT GCACCCATGA CCAAGAAGCA TTTGCAGGTC 
ACAGGCTATG GCGAGTTGGG ACTAGGCGAT GCCAACGACG TCTGGCGCGT GTTGATTGTG 
GGCGGCAAGG TCAACGAAAC GGTACACACG GTCACTTCCC GTCTCAAGTT CATCCACCTG 
CTGCAGAACT GCGCCCTGAC CTCCAGTGGG AAGCAGCTAC CGAAATGGGG GTTCGAACAG 
CAGGAGGTGT CATGCAATCC CAACGTGCGC GACAAGAACT CGCAGTGGAA CGTCGAGGAC 
AACGAGCACA AGTTGATGCC CAGTGTAAGC TTTAGCGTGT ATGCGCCCGG CTTCTTTGCC 
CGCTTCCTCG AATCCCACGC CGTAATGCTG CAGGGAAACG CAGGACTCAA GCCCAAAGAA 
GGCGAGGTCA CCAGCAGACC CTGGCAGTGG CCAATCAACT ACAGGGGCCA GTTCTTCTCC 
GGCAGCAGTT ACCGCATCTA CTTGCTGGGA AACCCGTTGA TTTGGTGGAG TAATCTGGTT 
TTCCTGGCCC TTTTCGTCAC GGTTTTCCTG TGCAACGCAG TTGTTCAGCA AAGAAGAGCT 
GGCTTCGCCC GATCGGCGGC CCAAAATCAA GCCCAAGTTC CCGATTCGGA AACAGTGGCC 
CAGGACGAGG AGAGTGAGCA CTCCACCACG GACATCTGCA GTTGTTGTAC TCCGGCGAAG 
GAGATAGTCC CAAAGGCAGT TCCCAGTGGG TCCCCGGAGG CACCCAACCC GGCGCAGTCC 
TTAAGGGCGG CCGCCTGGCT TTTTCTGGGC TGGATGCTCC ACTATTTGCC CTTCTGGGCG 
ATGGGACGGG TGCTCTACTT CCACCACTAT TTTCCGGCCT TGATCTTCAA CTCCTTGCTG 
ACGGGCGTTA TGTACAACTA CATTTTGAGG GTTCTGCCCA AATGGATTCA CCACGTTATC 
CTCGGACTGG TTCTCTCGAT CCTGGTGTAT AGTTTTGCCG CCTTCTCGCC ACTTGCTTAC 
GGAATGAGTG GTCCTCTAGC CAACGAGCCG AACTCTACAA TGTACAATCT CAAGTGGCTT 
TCTACCTGGG AGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tw cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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