vnd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	vnd
Entrez Gene ID	31003
Full Name	ventral nervous system defective
Synonyms	CG6172,Dm vnd,Dm-NK2,Dmel\CG6172,EC6,EG:118B3.1,HDC16822,NK-2,NK2,Nkx2,VND,Vnd,dNK-2,l(1)1Bf,l(1)EA142,l(1)EC6,l(1)GA100,l(1)GA122,l(1)RC24,l(1)VA208,l(1)VE769,lab,nk-2,vnd/NK-2
General protein information	Preferred Names ventral nervous system defective Names CG6172 gene product from transcript CG6172-RB CG6172-PA CG6172-PB HDC16822 ventral nerve cord defective ventral nervous system condensation defective ventral neuroblasts defective ventral neurons defective vnd-PA vnd-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 1B10-1B10\|1-0.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001042788.4	NP_001036253.1	ventral nervous system defective, isoform B
NM_057438.4	NP_476786.2	ventral nervous system defective, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NKX2-5 and MLC2v double-positive cells possess ventricular-like properties. The results demonstrate that the NKX2-5(eGFP/w) and MLC2v(mCherry/w) hPSCs provide a powerful model system to capture region-specific cardiac differentiation from early to late stages. Our study would facilitate subtype-specific cardiac development and functional analysis using the hPSC-derived sources.
Title: Isolation and characterization of ventricular-like cells derived from NKX2-5

the Neurogenic Ectoderm Enhancer (NEE) at vnd takes additional input from the complementary Dpp, dorsal, twist gradient via a conserved Schnurri/Mad/Medea silencer element (SSE) unlike NEEs at brk, sog, rho, and vn
Title: A Schnurri/Mad/Medea complex attenuates the dorsal-twist gradient readout at vnd.

Tthe vnd/NK-2 gene encodes an additional protein isoform (NK-2B) that differs from the known one (NK-2A) in its N-terminal domain.
Title: The alternative protein isoform NK2B, encoded by the vnd/NK-2 proneural gene, directly activates transcription and is expressed following the start of cells differentiation.

excludes Meltrin expression from the embryonic ventral neuroectoderm
Title: The disintegrin and metalloprotease Meltrin from Drosophila forms oligomers via its protein binding domain and is regulated by the homeobox protein VND during embryonic development.

Vnd segment-specifically affects ind expression; it represses ind in the tritocerebrum but positively regulates ind in the deutocerebrum by suppressing Msh
Title: Role of en and novel interactions between msh, ind, and vnd in dorsoventral patterning of the Drosophila brain and ventral nerve cord.

The following vnd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vnd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00376	NM_001042788.4 Latest version!	Drosophila melanogaster ventral nervous system defective (vnd), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa00377	NM_057438.4 Latest version!	Drosophila melanogaster ventral nervous system defective (vnd), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vnd ( NM_001042788.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036253.1 Drosophila melanogaster ventral nervous system defective (vnd), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00376
Clone ID Related Accession (Same CDS sequence)	NM_001042788.4
Accession Version	NM_001042788.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ventral nervous system defective, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001042788.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAATT ATAATCCGAA TTGCGGTTAC TTCGAGGATT GCAGCTACTA CACGAACAAC 
GTGTTTCAAC AGGATTATTG CATGCAGACG GATTACGAGT ACAACAAGCA TGTCTACGAC 
TTATTCGATG CAAAGGTGCC GTCGTCCCAA CGCTCTGGCT TCCATATATC GGACATCTTG 
AATTTGGAGG GCTCTGAGCT GAAGAATGCA GCAGCTGCCG CCGCTGCTGC CGCCCACCAT 
GGCAGCGATT TGAGTCACCA CTCGGCCAGT GAGTCCACCA GTGGACATCG CGGCCAGGGA 
TCTCACACCT CGCCTTCGGC GCTGTCGCCC ACGCCAGCGG GCGTTTCCGC GGACGAGCAC 
CACAATGGCA GCGGGACTGG GGGAGGAGCG GGAGAAGCGG ATCACCACAG CACCACCGAG 
CATCACGCCC CTCCTAGCCA TCCGCAGCAA CAGCACCCGC ACCACCAGCA GCACCACCAC 
CCTCACCTCC TCCTCCCGCA GCAGCACCAT CAGCAGGCGG TGGCCCCACT CCCTTTGGCG 
CACCATCAGA GCGGCGAAGC CCAAAGCCAC GCCCATGCCA ATGCAGCCGC CGCCCATCTG 
CTGGCCAGCC ACAATGCAGC GGCTGCGGCT GCTGTGGCCG CCGGCCAATA TCTGCCCAAC 
CTGCCCAAGA ACTTCCCGGG GAGCTTTGGC GACGAGATGT CCTCGTACCA CCACATGGCC 
CAAACCATGC TGCAGCACTC GGGCAGGAGT GCGTGGATCA AGGAGAACGA GCTATACGGT 
ACCCAGCAGC CCGCCAGTCC GGATAGCACC TCCCCAGTTA CCTCGGAAGT GTCGTACACC 
TACATTGGTT CCAACTGCCA GACATCGCCT GCCCTTTCCG GCGATTACAA GAGCTACAGC 
CGGTCGGCCG ATAGCGATGC ACTATCCGTG GGCGACGCCC TGCACACCCT CCATGGATCC 
TCTGGTAATG GAAGTGCCGG AGGCGCTCCG ACGGCCCATG CCCTACACAA CAACAATAAT 
AATACGACAA ACAACAATAA CCACAGCCTG AAGGCCGAGG GGATCAACGG AGCAGGCAGT 
GGTCACGACG ATAGCCTCAA CGAAGATGGC ATCGAGGAGG ATATCGACGA CGTGGACGAC 
GCCGACGGCA GTGGCGGCGG GGATGCAAAT GGATCCGACG GTCTGCCAAA TAAGAAACGG 
AAGCGACGAG TCCTGTTCAC CAAGGCGCAA ACATATGAGC TGGAACGTCG GTTTCGACAA 
CAACGTTACT TGAGTGCCCC GGAACGCGAG CACCTGGCCA GTTTGATCCG CCTGACGCCG 
ACCCAGGTGA AGATCTGGTT TCAAAACCAT CGCTACAAGA CGAAGCGGGC GCAAAACGAG 
AAGGGCTACG AGGGTCATCC TGGTCTACTG CACGGCCATG CCACCCATCC GCATCACCCC 
AGTGCCCTGC CATCGCCCCG TCGGGTAGCC GTTCCAGTTC TGGTGAGGAA CGGAAAGCCC 
TGCTTGGGCG ATAGTTCCAA ACTGGGAGCC GACTGCGTCT CCGTGTCATC AGCCACCGCC 
ACCGCCATGC AGAATGCCGC CGCCCATCAC TTGGTTGCCC TAAATGGAGC GGCCGCCTAT 
CAACATGCCG CTGCAGCGGC TGCCGGCCTT CACGCCCACG CCCATGCTCA TGCCCACGCC 
CACGGACACG GCCATCCACA CGCCCACGCC CAGAGGGCCG CCTGGTGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036253.1
CDS	839..2572
Translation	MSNYNPNCGYFEDCSYYTNNVFQQDYCMQTDYEYNKHVYDLFDAKVPSSQRSGFHISDILNLEGSELKNAAAAAAAAAHHGSDLSHHSASESTSGHRGQGSHTSPSALSPTPAGVSADEHHNGSGTGGGAGEADHHSTTEHHAPPSHPQQQHPHHQQHHHPHLLLPQQHHQQAVAPLPLAHHQSGEAQSHAHANAAAAHLLASHNAAAAAAVAAGQYLPNLPKNFPGSFGDEMSSYHHMAQTMLQHSGRSAWIKENELYGTQQPASPDSTSPVTSEVSYTYIGSNCQTSPALSGDYKSYSRSADSDALSVGDALHTLHGSSGNGSAGGAPTAHALHNNNNNTTNNNNHSLKAEGINGAGSGHDDSLNEDGIEEDIDDVDDADGSGGGDANGSDGLPNKKRKRRVLFTKAQTYELERRFRQQRYLSAPEREHLASLIRLTPTQVKIWFQNHRYKTKRAQNEKGYEGHPGLLHGHATHPHHPSALPSPRRVAVPVLVRNGKPCLGDSSKLGADCVSVSSATATAMQNAAAHHLVALNGAAAYQHAAAAAAGLHAHAHAHAHAHGHGHPHAHAQRAAWWP

Target ORF information:

RefSeq Version	NM_001042788.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ventral nervous system defective (vnd), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042788.4

ORF Insert Sequence:

ATGAGCAATT ATAATCCGAA TTGCGGTTAC TTCGAGGATT GCAGCTACTA CACGAACAAC 
GTGTTTCAAC AGGATTATTG CATGCAGACG GATTACGAGT ACAACAAGCA TGTCTACGAC 
TTATTCGATG CAAAGGTGCC GTCGTCCCAA CGCTCTGGCT TCCATATATC GGACATCTTG 
AATTTGGAGG GCTCTGAGCT GAAGAATGCA GCAGCTGCCG CCGCTGCTGC CGCCCACCAT 
GGCAGCGATT TGAGTCACCA CTCGGCCAGT GAGTCCACCA GTGGACATCG CGGCCAGGGA 
TCTCACACCT CGCCTTCGGC GCTGTCGCCC ACGCCAGCGG GCGTTTCCGC GGACGAGCAC 
CACAATGGCA GCGGGACTGG GGGAGGAGCG GGAGAAGCGG ATCACCACAG CACCACCGAG 
CATCACGCCC CTCCTAGCCA TCCGCAGCAA CAGCACCCGC ACCACCAGCA GCACCACCAC 
CCTCACCTCC TCCTCCCGCA GCAGCACCAT CAGCAGGCGG TGGCCCCACT CCCTTTGGCG 
CACCATCAGA GCGGCGAAGC CCAAAGCCAC GCCCATGCCA ATGCAGCCGC CGCCCATCTG 
CTGGCCAGCC ACAATGCAGC GGCTGCGGCT GCTGTGGCCG CCGGCCAATA TCTGCCCAAC 
CTGCCCAAGA ACTTCCCGGG GAGCTTTGGC GACGAGATGT CCTCGTACCA CCACATGGCC 
CAAACCATGC TGCAGCACTC GGGCAGGAGT GCGTGGATCA AGGAGAACGA GCTATACGGT 
ACCCAGCAGC CCGCCAGTCC GGATAGCACC TCCCCAGTTA CCTCGGAAGT GTCGTACACC 
TACATTGGTT CCAACTGCCA GACATCGCCT GCCCTTTCCG GCGATTACAA GAGCTACAGC 
CGGTCGGCCG ATAGCGATGC ACTATCCGTG GGCGACGCCC TGCACACCCT CCATGGATCC 
TCTGGTAATG GAAGTGCCGG AGGCGCTCCG ACGGCCCATG CCCTACACAA CAACAATAAT 
AATACGACAA ACAACAATAA CCACAGCCTG AAGGCCGAGG GGATCAACGG AGCAGGCAGT 
GGTCACGACG ATAGCCTCAA CGAAGATGGC ATCGAGGAGG ATATCGACGA CGTGGACGAC 
GCCGACGGCA GTGGCGGCGG GGATGCAAAT GGATCCGACG GTCTGCCAAA TAAGAAACGG 
AAGCGACGAG TCCTGTTCAC CAAGGCGCAA ACATATGAGC TGGAACGTCG GTTTCGACAA 
CAACGTTACT TGAGTGCCCC GGAACGCGAG CACCTGGCCA GTTTGATCCG CCTGACGCCG 
ACCCAGGTGA AGATCTGGTT TCAAAACCAT CGCTACAAGA CGAAGCGGGC GCAAAACGAG 
AAGGGCTACG AGGGTCATCC TGGTCTACTG CACGGCCATG CCACCCATCC GCATCACCCC 
AGTGCCCTGC CATCGCCCCG TCGGGTAGCC GTTCCAGTTC TGGTGAGGAA CGGAAAGCCC 
TGCTTGGGCG ATAGTTCCAA ACTGGGAGCC GACTGCGTCT CCGTGTCATC AGCCACCGCC 
ACCGCCATGC AGAATGCCGC CGCCCATCAC TTGGTTGCCC TAAATGGAGC GGCCGCCTAT 
CAACATGCCG CTGCAGCGGC TGCCGGCCTT CACGCCCACG CCCATGCTCA TGCCCACGCC 
CACGGACACG GCCATCCACA CGCCCACGCC CAGAGGGCCG CCTGGTGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vnd ( NM_057438.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476786.2 Drosophila melanogaster ventral nervous system defective (vnd), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00377
Clone ID Related Accession (Same CDS sequence)	NM_057438.4
Accession Version	NM_057438.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ventral nervous system defective, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057438.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCACGT CGGCGTCCTT GGAGAGGACC CCCTCAAAGC GGGATCGAGA TCGCGAGCGG 
GACAACAGCA GCGGTCTGGG CAGCGCTGGC AGCTTGCCCG CATCGCCCCA AAGCGCTATC 
ACGGTGAGTC CGTCCTCCCC AGCCACGCCG AAGCGCCCGC TGCGCACCTC AACGCCCTCG 
CTGGAGCGGA AGAGGGAGCG TGAGGATCGA GAGGACCGGG AGGATCGCAA GGAGCGGCAG 
GAACGGCACG AGCGAGACAG AGATCACGAG AGATTCGCCG CAGTCTTCAG CACCGCCAGC 
ACCACCGTGC CCACGAACAC AAGTTCCAGT TCCGGATTGG CGCCCGAACA GCTCCGCATT 
CCGACGGGTG CAGCCGCCTT CAGCGGATTT CCGGGGCTCC ACAGCATGAG TAGTCTTATG 
CTTCCATCGT CGGCGGCTGT GGCCGCCGCA GCAGCGGCCC CGTTTCTGCC CTGGTCGCCC 
ATCCTGCTGC CGCCGTGGAA CCACGCCCTC CTACCAGCCG CCTTTTATCC GGCGGCCCTG 
CGAAACGCTT TGCCTGGCTT ATTCGATGCA AAGGTGCCGT CGTCCCAACG CTCTGGCTTC 
CATATATCGG ACATCTTGAA TTTGGAGGGC TCTGAGCTGA AGAATGCAGC AGCTGCCGCC 
GCTGCTGCCG CCCACCATGG CAGCGATTTG AGTCACCACT CGGCCAGTGA GTCCACCAGT 
GGACATCGCG GCCAGGGATC TCACACCTCG CCTTCGGCGC TGTCGCCCAC GCCAGCGGGC 
GTTTCCGCGG ACGAGCACCA CAATGGCAGC GGGACTGGGG GAGGAGCGGG AGAAGCGGAT 
CACCACAGCA CCACCGAGCA TCACGCCCCT CCTAGCCATC CGCAGCAACA GCACCCGCAC 
CACCAGCAGC ACCACCACCC TCACCTCCTC CTCCCGCAGC AGCACCATCA GCAGGCGGTG 
GCCCCACTCC CTTTGGCGCA CCATCAGAGC GGCGAAGCCC AAAGCCACGC CCATGCCAAT 
GCAGCCGCCG CCCATCTGCT GGCCAGCCAC AATGCAGCGG CTGCGGCTGC TGTGGCCGCC 
GGCCAATATC TGCCCAACCT GCCCAAGAAC TTCCCGGGGA GCTTTGGCGA CGAGATGTCC 
TCGTACCACC ACATGGCCCA AACCATGCTG CAGCACTCGG GCAGGAGTGC GTGGATCAAG 
GAGAACGAGC TATACGGTAC CCAGCAGCCC GCCAGTCCGG ATAGCACCTC CCCAGTTACC 
TCGGAAGTGT CGTACACCTA CATTGGTTCC AACTGCCAGA CATCGCCTGC CCTTTCCGGC 
GATTACAAGA GCTACAGCCG GTCGGCCGAT AGCGATGCAC TATCCGTGGG CGACGCCCTG 
CACACCCTCC ATGGATCCTC TGGTAATGGA AGTGCCGGAG GCGCTCCGAC GGCCCATGCC 
CTACACAACA ACAATAATAA TACGACAAAC AACAATAACC ACAGCCTGAA GGCCGAGGGG 
ATCAACGGAG CAGGCAGTGG TCACGACGAT AGCCTCAACG AAGATGGCAT CGAGGAGGAT 
ATCGACGACG TGGACGACGC CGACGGCAGT GGCGGCGGGG ATGCAAATGG ATCCGACGGT 
CTGCCAAATA AGAAACGGAA GCGACGAGTC CTGTTCACCA AGGCGCAAAC ATATGAGCTG 
GAACGTCGGT TTCGACAACA ACGTTACTTG AGTGCCCCGG AACGCGAGCA CCTGGCCAGT 
TTGATCCGCC TGACGCCGAC CCAGGTGAAG ATCTGGTTTC AAAACCATCG CTACAAGACG 
AAGCGGGCGC AAAACGAGAA GGGCTACGAG GGTCATCCTG GTCTACTGCA CGGCCATGCC 
ACCCATCCGC ATCACCCCAG TGCCCTGCCA TCGCCCCGTC GGGTAGCCGT TCCAGTTCTG 
GTGAGGAACG GAAAGCCCTG CTTGGGCGAT AGTTCCAAAC TGGGAGCCGA CTGCGTCTCC 
GTGTCATCAG CCACCGCCAC CGCCATGCAG AATGCCGCCG CCCATCACTT GGTTGCCCTA 
AATGGAGCGG CCGCCTATCA ACATGCCGCT GCAGCGGCTG CCGGCCTTCA CGCCCACGCC 
CATGCTCATG CCCACGCCCA CGGACACGGC CATCCACACG CCCACGCCCA GAGGGCCGCC 
TGGTGGCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476786.2
CDS	354..2525
Translation	MTTSASLERTPSKRDRDRERDNSSGLGSAGSLPASPQSAITVSPSSPATPKRPLRTSTPSLERKREREDREDREDRKERQERHERDRDHERFAAVFSTASTTVPTNTSSSSGLAPEQLRIPTGAAAFSGFPGLHSMSSLMLPSSAAVAAAAAAPFLPWSPILLPPWNHALLPAAFYPAALRNALPGLFDAKVPSSQRSGFHISDILNLEGSELKNAAAAAAAAAHHGSDLSHHSASESTSGHRGQGSHTSPSALSPTPAGVSADEHHNGSGTGGGAGEADHHSTTEHHAPPSHPQQQHPHHQQHHHPHLLLPQQHHQQAVAPLPLAHHQSGEAQSHAHANAAAAHLLASHNAAAAAAVAAGQYLPNLPKNFPGSFGDEMSSYHHMAQTMLQHSGRSAWIKENELYGTQQPASPDSTSPVTSEVSYTYIGSNCQTSPALSGDYKSYSRSADSDALSVGDALHTLHGSSGNGSAGGAPTAHALHNNNNNTTNNNNHSLKAEGINGAGSGHDDSLNEDGIEEDIDDVDDADGSGGGDANGSDGLPNKKRKRRVLFTKAQTYELERRFRQQRYLSAPEREHLASLIRLTPTQVKIWFQNHRYKTKRAQNEKGYEGHPGLLHGHATHPHHPSALPSPRRVAVPVLVRNGKPCLGDSSKLGADCVSVSSATATAMQNAAAHHLVALNGAAAYQHAAAAAAGLHAHAHAHAHAHGHGHPHAHAQRAAWWP

Target ORF information:

RefSeq Version	NM_057438.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ventral nervous system defective (vnd), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057438.4

ORF Insert Sequence:

ATGACCACGT CGGCGTCCTT GGAGAGGACC CCCTCAAAGC GGGATCGAGA TCGCGAGCGG 
GACAACAGCA GCGGTCTGGG CAGCGCTGGC AGCTTGCCCG CATCGCCCCA AAGCGCTATC 
ACGGTGAGTC CGTCCTCCCC AGCCACGCCG AAGCGCCCGC TGCGCACCTC AACGCCCTCG 
CTGGAGCGGA AGAGGGAGCG TGAGGATCGA GAGGACCGGG AGGATCGCAA GGAGCGGCAG 
GAACGGCACG AGCGAGACAG AGATCACGAG AGATTCGCCG CAGTCTTCAG CACCGCCAGC 
ACCACCGTGC CCACGAACAC AAGTTCCAGT TCCGGATTGG CGCCCGAACA GCTCCGCATT 
CCGACGGGTG CAGCCGCCTT CAGCGGATTT CCGGGGCTCC ACAGCATGAG TAGTCTTATG 
CTTCCATCGT CGGCGGCTGT GGCCGCCGCA GCAGCGGCCC CGTTTCTGCC CTGGTCGCCC 
ATCCTGCTGC CGCCGTGGAA CCACGCCCTC CTACCAGCCG CCTTTTATCC GGCGGCCCTG 
CGAAACGCTT TGCCTGGCTT ATTCGATGCA AAGGTGCCGT CGTCCCAACG CTCTGGCTTC 
CATATATCGG ACATCTTGAA TTTGGAGGGC TCTGAGCTGA AGAATGCAGC AGCTGCCGCC 
GCTGCTGCCG CCCACCATGG CAGCGATTTG AGTCACCACT CGGCCAGTGA GTCCACCAGT 
GGACATCGCG GCCAGGGATC TCACACCTCG CCTTCGGCGC TGTCGCCCAC GCCAGCGGGC 
GTTTCCGCGG ACGAGCACCA CAATGGCAGC GGGACTGGGG GAGGAGCGGG AGAAGCGGAT 
CACCACAGCA CCACCGAGCA TCACGCCCCT CCTAGCCATC CGCAGCAACA GCACCCGCAC 
CACCAGCAGC ACCACCACCC TCACCTCCTC CTCCCGCAGC AGCACCATCA GCAGGCGGTG 
GCCCCACTCC CTTTGGCGCA CCATCAGAGC GGCGAAGCCC AAAGCCACGC CCATGCCAAT 
GCAGCCGCCG CCCATCTGCT GGCCAGCCAC AATGCAGCGG CTGCGGCTGC TGTGGCCGCC 
GGCCAATATC TGCCCAACCT GCCCAAGAAC TTCCCGGGGA GCTTTGGCGA CGAGATGTCC 
TCGTACCACC ACATGGCCCA AACCATGCTG CAGCACTCGG GCAGGAGTGC GTGGATCAAG 
GAGAACGAGC TATACGGTAC CCAGCAGCCC GCCAGTCCGG ATAGCACCTC CCCAGTTACC 
TCGGAAGTGT CGTACACCTA CATTGGTTCC AACTGCCAGA CATCGCCTGC CCTTTCCGGC 
GATTACAAGA GCTACAGCCG GTCGGCCGAT AGCGATGCAC TATCCGTGGG CGACGCCCTG 
CACACCCTCC ATGGATCCTC TGGTAATGGA AGTGCCGGAG GCGCTCCGAC GGCCCATGCC 
CTACACAACA ACAATAATAA TACGACAAAC AACAATAACC ACAGCCTGAA GGCCGAGGGG 
ATCAACGGAG CAGGCAGTGG TCACGACGAT AGCCTCAACG AAGATGGCAT CGAGGAGGAT 
ATCGACGACG TGGACGACGC CGACGGCAGT GGCGGCGGGG ATGCAAATGG ATCCGACGGT 
CTGCCAAATA AGAAACGGAA GCGACGAGTC CTGTTCACCA AGGCGCAAAC ATATGAGCTG 
GAACGTCGGT TTCGACAACA ACGTTACTTG AGTGCCCCGG AACGCGAGCA CCTGGCCAGT 
TTGATCCGCC TGACGCCGAC CCAGGTGAAG ATCTGGTTTC AAAACCATCG CTACAAGACG 
AAGCGGGCGC AAAACGAGAA GGGCTACGAG GGTCATCCTG GTCTACTGCA CGGCCATGCC 
ACCCATCCGC ATCACCCCAG TGCCCTGCCA TCGCCCCGTC GGGTAGCCGT TCCAGTTCTG 
GTGAGGAACG GAAAGCCCTG CTTGGGCGAT AGTTCCAAAC TGGGAGCCGA CTGCGTCTCC 
GTGTCATCAG CCACCGCCAC CGCCATGCAG AATGCCGCCG CCCATCACTT GGTTGCCCTA 
AATGGAGCGG CCGCCTATCA ACATGCCGCT GCAGCGGCTG CCGGCCTTCA CGCCCACGCC 
CATGCTCATG CCCACGCCCA CGGACACGGC CATCCACACG CCCACGCCCA GAGGGCCGCC 
TGGTGGCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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vnd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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