mim cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following mim gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mim cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa11766 NM_001014500.3
Latest version!
Drosophila melanogaster missing-in-metastasis (mim), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OFa11767 NM_001103735.3
Latest version!
Drosophila melanogaster missing-in-metastasis (mim), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OFa12117 NM_001273819.1
Latest version!
Drosophila melanogaster missing-in-metastasis (mim), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OFa12118 NM_001201941.2
Latest version!
Drosophila melanogaster missing-in-metastasis (mim), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OFa12119 NM_001169597.2
Latest version!
Drosophila melanogaster missing-in-metastasis (mim), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa12120 NM_001201940.3
Latest version!
Drosophila melanogaster missing-in-metastasis (mim), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OFa12121 NM_001273818.1
Latest version!
Drosophila melanogaster missing-in-metastasis (mim), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa12122 NM_001273820.1
Latest version!
Drosophila melanogaster missing-in-metastasis (mim), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa12123 NM_001201939.3
Latest version!
Drosophila melanogaster missing-in-metastasis (mim), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa12124 NM_001259228.2
Latest version!
Drosophila melanogaster missing-in-metastasis (mim), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OFa12125 NM_001273821.1
Latest version!
Drosophila melanogaster missing-in-metastasis (mim), transcript variant N, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OFa12126 NM_001259230.2
Latest version!
Drosophila melanogaster missing-in-metastasis (mim), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OFa12127 NM_001259229.2
Latest version!
Drosophila melanogaster missing-in-metastasis (mim), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa11766
    Clone ID Related Accession (Same CDS sequence) NM_001014500.3
    Accession Version NM_001014500.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6084bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product missing-in-metastasis, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001014500.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GACTTCGAAT GCAACGAACC AGACGGCAAA TGTCTCCACC
    TGGCCCCCAC ATTCCCAGGA TGGCGTCGAC ACCCTGCCAC CGACCGCTGA CCGTCCGCAC
    ACGATTTCGA CGGCATACGA AAAGGGTCAC CAGCGTCCGC CGCTGACCGT CTACACGTTC
    CAAAACCCGG AGACCATTCA CGAGTCGGGC AGCTGCCTGA ACAACGGAAC AGCAGCCCCG
    AATGGACAGC CGTTATCCGG ACAAGCCACT CCGGCCACCC AGAAATCACC GGCTGCCTCA
    CTTAGCCGGC CTCCTTTGCC AGTTCGCTGC TCGTCGTTGG AGCGACCCCT TTCGGCCCAG
    AGTAACCACC GCCAGGGTAG CGGGAATAAC CTGCTGCAGC GCCAGTGCCC CTCGCCGATT
    CCGGCTCATA TCACGAAAGA GCTGTCCGCA GCGCATCATG CACAGCAGCA GCAGCAGCAG
    CAAAATCAGC AGCCCCAGAC GCCGCCCACC TATGTGAACA TGTCGGAGCT GGCCACCATG
    GCCGCTTTGA AGCAGACCAA TCAGCAGCAG AAGCCCTCTA CGCCGCCTCT GCAGCAGCAG
    AGCTCCATTG ACTCGACCAG CTCCCAGCAT TCCAACGACT CCACCGGCTC TCATCAGCTC
    CTCCAGCAGC AGCAGCAGCA TCATCAATCG CAGCAGAATC ACCACTCAGC CACTGCCACA
    CGCTCCCATT CCATATCCTC GACGGCCTCG TCACTTCACT CGCATCCGTC GATCGACTCC
    ACCGTCGCTT GCGGCTCGCT GGTGGGCCAG CACAACCACA GCACCAGCAC CAACACGAAC
    ACCACCTCGC CGTCCAGTGG CAGCTCCACG CCACAGAACC ATTACTCGCC CCTGCTAACC
    AACTCCCCCA CGTCCACTGC CGCAGGTACT CCCAGTGGCA GCAGCTTAGG TCCTGGGTCC
    GGTTTGGGAT TTGTCTACCA GGTCAGCTCC CCGACGCCTC CCTCCAGCGA GGTGCTGAAA
    ATCACCGAGC AAGCCGCTGC AGGACAGGAT CAGGGTCCAG CCAACAGCGT AGCGGACGAG
    ACGGATGAGC GATCAAGGGC CTCAGTCCTG CAGAAGGCTT CAATGTTCGA AAAGGCGGCA
    GCAGCGGCAG CGGTATCGCC CCCAGCTCCC ATGCAGATTG CATCCGGTTC CCCAGCTTCG
    GGAGGCGGAA CTCGACGATC CGAGGCGGAG CAGCAGGAAA TGGACAAGTC TTTCGAAGAT
    TCAATACAAG CACTAAATAA TTTAATTGGC GAACTAGACT CGTTCCAACG CGAGATCGAT
    GAGGGCAAGG TCAAACCGCC GAGCAACATC ATAAGCGGCA GCACCACCAG TAGCAACAAC
    AACAATACGA CGACCAGCAG CATCAGCAGC AGCGACAACA ACAACCTGCC CGCCACCAGC
    AACATCGAGC CATGCGCCAT CAGCAATCAG ACGAACTCGA GCGGCTGCGG CACGGACATA
    TCCGACACCA CGTCCGACGA ACTGGCCGGC GACGATATGG ACGTCAGGCG GCGGGATCGG
    GATCGGGACC GGGATCTGCT GGGCGCCAGC GATTCGGAGC TGAGTCGCTG CTATGTGAGC
    GAGACGAGTT CGCTGACCGG TGGCCTAACG GCCGGCGGCT ACGAGAATCC CACTTTCGCG
    CACTTTGCGG CCAATGCCAA TCGGGAGGAC GCTGTTTCGC TGGCCTCCGA CAGCGTTTGT
    CTCGGCCAGC CACGCCACGC CTATGTGGAT ACCTGCAGCG ACAGCGGCAG TGCCGTGGTG
    GTGATCTACG ACCACCAGAT TCCCAACACA CCCGACATTG AGTTCGTGAA GCAGAACTCT
    GAGATTGTAG TGCTGCGGAC TAAGGATCCG CAGCCCCACG CGCTGCAGCT GCACGAGATG
    CGCGAGCTGC AGCAGTTGCC CGCCAATTTG GCCGGTTCGC CGGACTCCTC GCCGGACTCT
    GCCGGTGGCC AGGCACCGCC AACAGCAACT GTGGCGCCCG CCAAGCAGCG ACTCTCCTCG
    TTTCGCGCCA CCAGTGAGCA GCAGTTGCAG CTCCTCGGAC GCGGTAGCCC GCAAAGAGGT
    AAAACACCCA GTGAGCAGGC GGTACAAAGC AGGCCACAGG ACCAGCATTT TCCACAGACA
    CAACAGCAGG ATATTGATGG CAGTAGTCCA CCAGTAGAAC TTGCAAGGCG CCAGCTGCCC
    CCCAAGCCCA CCAGTTTGAG TATTTTTAAC GGCCCCGTGC CCACTGCGGG CGATAGGCCT
    GTCGTGCCGC GAAAGTCGGA CTTTAAGGCT GATCTAGATG CTAAAATACG CAGGCAAAAG
    CAGAAGGTTA AACAGCAGTT GCAGCAGCAG CAGCAGCAAC AGCAGCAAGA GCAGCAAGAA
    ACGCAGCAGC AGCAGCAAGC ACCACAAGAA CAGCAACACT CACCACAGTC GCCCCAAACC
    AGAAACTGTA ATGTCACTAA TCAACAAGCC GCCAATATTA CTGCATCAGC ATCTGCATTT
    GCAACCGCAA CAGCATCCAC AGACCCGTAC CCGAATCCAA ATCATAGAAT GCCAAACCAA
    AATCAGACAG CCACATCCAA TCACACGCAG TGCAAGACGC CCACAATGGC ATTGTCACCG
    TCATCACCTC GCGGCCATTT GCCATTATCA TCGTCATCGC TATCGTCATT ACCATTACCA
    GCCACCACTT CATCACCATC AAATGCCCGG CCATCGATGT TGCCCGCCAG TGACCGACCA
    CCCGCCCATC CATATGTGTG CTCCAATGCC CCAGCCAATC CCCACCACGC CAATAGCATT
    TCCAATGCCA ATGCCCATCT CAAGCCGTGC ATTACGCCCC GGCCGGCTTC TTTGTCGGGA
    GGAGCAGCCG GCGGTGGCTC CACGCGCATC GGGCGTCGAT CGTCCATTAA TCAGTCCAAG
    CCACCGCCGC CAGTCAGACG CAGTTCGTCG GTGACCCCCA GTCCCAATGC CTCGGTCGGG
    CTGCAGCAGC AACCACAGCA CGCGACTCTG TCGCAGCAGA ATCACCAACT AAGCAGCTCC
    AGCGAGCACT TACCGCCGCC ACCGCCATTC ATGCTGGACG CCATGCCCCA GATTCCCAGC
    TCAGCGCTGA AGGTATCGGA GACGGTAAGA GCCCTGGCAG CCATGCGGCA CCAGCCAGCA
    TCACCTGTGT CCCTCAGACG CATGCAGCAG CAACAGCAGC AGCAGCTTCA GCAGCAACAG
    CAGCAACAGG TGCAGCAGCA GCAACCCCTA TTGCAGTCTG CGCACAACTC CCCCCTCAAA
    GAGGACCTGA CCGTGTACTA CGACTCCTAC TTGGATCTGC ACGCCTATGC CCAGGCCTTG
    GCCAGCGGCC AACAGCCCGG CGGTCAGCAG ATGGCCAACC AGCAACGCTT TACCCTCCAG
    CAGCAACATC AGCATCTGCA TCAGCCACAG CAACCGCCTG TCTACCAAGT CGATGCCACG
    TTCCGCACCT CATCACCAGC CGCGGGCGGA GGGGGTGGCG GCGGCATATA CGCCCAGCCC
    AAACTGGTCA ACAGCATGTC CAGCTTCCGC ACCAGCAGCC CCAGTCCCAA CGGACATGCT
    CACCCACTGC CACCGACACA GCCAAAGGCG AATCCGAACC TAATTGCACA GCTCAATGCA
    CGACTCAGCG GCAAACAGCA GCAGCACCAA CAGCAGCAGC ATATCGAGGG GATCTACGGC
    AACCAGCAGG CGCCCGGAGG AGAGTCGATC TACATGCGGA GTGGCCTGTC CATGTCGCAG
    CCGCAACAGC AGCAACACTT TGACGGTAAA TCTGAACAAA TCCAGCTGCA GCACCAGCAA
    CAGCATAGAA TTTACGCTAG TTTCGGCACC TCATCATCAG CAATGTCATC CGCTCATGCG
    GCCAACAGCA GCACTAAACC GTCCATTCTA ACACCGACCA CCTCTTTCAA TGCATTGCCT
    CACTTCCCCC TGTCTTCATC CACATCATCG TTGCTCTCCA AAGTCAGCTC ATTCTCGAAC
    TCTTCATCCG CATCCCCACC GACAACGGCA GCGACCTCTG GCTCGGCCAG TTCGCATTAT
    CAGCCACCTC AGCCGCCGAA TGCAGCAGTT GCTAACAGCA AAGACATGGC CATCTACTCA
    AGTTCGTTTA CCAAAAATCC AGCAGCTGCG CAATCGCCGA ACATGAGACA GGCTCATTCC
    CATCAGCACC ACCAGCCGCC GCAGCAGCAG CACTACACCT GTCCGCCTCC TCTGGAGGAT
    CCCCCACCGC CGCCCATTTA CGCCGCCGGT GCATCGGCCA CGATGCCCAA GAAGATGGCC
    CGTCCGCCCA CTGGCCAGAA CGCGACTCAC TCGAGCGCCT ATGCAGCAGC CTCGTCTACG
    GCCACGCTGC CCAAGAATAT GATGCAGCAG CAGCAGCGGT TGCAGCACCA GCAGCAGTAT
    CAACAGCCGG CAGGCATGGG CATTGGCAAC GGCAATGGGC ACCTAGGTCA GCGTCCGCAG
    TTGCCGCTGC CCCAGCAGAA GCTTAGAGCT GCACAGCAGC AGCACTTGGC GGAGCAGCAG
    CATCAGCAAC AGCAAGAACA GCAGCAGCAC CACCAGCAGC ACCAACAACA CCAGCAACGC
    CAGCCGCCCA TTCCTTCACG CCACTCAAGT GTGCAGCAAA AGATATTCGT ATCAACGAAT
    CCATTCATAC AGACAACGGC CGTCAAGTTT CACTCGCCCT CGGCCTCGCC CACGTGCGGC
    TCGCCCGTTA CTGGGTCCTT GGCTAGCATT TATGCCACAA CCTCGCGTGG CGGCCACCAT
    CACCAGCAGC AGCAGGCTCA GCAGCAGCAG CACTACTATC GCGATGCTGC CGGGGGCAAC
    AGCAACGGCG GCGCTGCCTA CTATAACCAC AATGCCCATG CCCATTCCCA GGCACATCAT
    CCAAACTATG CCACAAGCAC AAATATCGAA AAGACTGGCA GTATTCGTGC CAAGACCAAG
    GCCGAATTCC TCGAGAATCT CAACGCGAAG CTGGCGAAGC AGGGAATGTC TGGACGAGCA
    TTTGCCGTGC GAAATCTCAT CAACAGCAAG GCCCTGCCGG ACCCTCGCAT CTGTCACGAA
    TCGCTGATGG ATCAAATCAA GCGCGGAGCC ACCTTAAAGC GTAACCAGAA GATCAACGAT
    CGCAGCGCTC CCAAGATACA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001014500.2
    CDS890..6973
    Translation

    Target ORF information:

    RefSeq Version NM_001014500.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster missing-in-metastasis (mim), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001014500.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GACTTCGAAT GCAACGAACC AGACGGCAAA TGTCTCCACC
    TGGCCCCCAC ATTCCCAGGA TGGCGTCGAC ACCCTGCCAC CGACCGCTGA CCGTCCGCAC
    ACGATTTCGA CGGCATACGA AAAGGGTCAC CAGCGTCCGC CGCTGACCGT CTACACGTTC
    CAAAACCCGG AGACCATTCA CGAGTCGGGC AGCTGCCTGA ACAACGGAAC AGCAGCCCCG
    AATGGACAGC CGTTATCCGG ACAAGCCACT CCGGCCACCC AGAAATCACC GGCTGCCTCA
    CTTAGCCGGC CTCCTTTGCC AGTTCGCTGC TCGTCGTTGG AGCGACCCCT TTCGGCCCAG
    AGTAACCACC GCCAGGGTAG CGGGAATAAC CTGCTGCAGC GCCAGTGCCC CTCGCCGATT
    CCGGCTCATA TCACGAAAGA GCTGTCCGCA GCGCATCATG CACAGCAGCA GCAGCAGCAG
    CAAAATCAGC AGCCCCAGAC GCCGCCCACC TATGTGAACA TGTCGGAGCT GGCCACCATG
    GCCGCTTTGA AGCAGACCAA TCAGCAGCAG AAGCCCTCTA CGCCGCCTCT GCAGCAGCAG
    AGCTCCATTG ACTCGACCAG CTCCCAGCAT TCCAACGACT CCACCGGCTC TCATCAGCTC
    CTCCAGCAGC AGCAGCAGCA TCATCAATCG CAGCAGAATC ACCACTCAGC CACTGCCACA
    CGCTCCCATT CCATATCCTC GACGGCCTCG TCACTTCACT CGCATCCGTC GATCGACTCC
    ACCGTCGCTT GCGGCTCGCT GGTGGGCCAG CACAACCACA GCACCAGCAC CAACACGAAC
    ACCACCTCGC CGTCCAGTGG CAGCTCCACG CCACAGAACC ATTACTCGCC CCTGCTAACC
    AACTCCCCCA CGTCCACTGC CGCAGGTACT CCCAGTGGCA GCAGCTTAGG TCCTGGGTCC
    GGTTTGGGAT TTGTCTACCA GGTCAGCTCC CCGACGCCTC CCTCCAGCGA GGTGCTGAAA
    ATCACCGAGC AAGCCGCTGC AGGACAGGAT CAGGGTCCAG CCAACAGCGT AGCGGACGAG
    ACGGATGAGC GATCAAGGGC CTCAGTCCTG CAGAAGGCTT CAATGTTCGA AAAGGCGGCA
    GCAGCGGCAG CGGTATCGCC CCCAGCTCCC ATGCAGATTG CATCCGGTTC CCCAGCTTCG
    GGAGGCGGAA CTCGACGATC CGAGGCGGAG CAGCAGGAAA TGGACAAGTC TTTCGAAGAT
    TCAATACAAG CACTAAATAA TTTAATTGGC GAACTAGACT CGTTCCAACG CGAGATCGAT
    GAGGGCAAGG TCAAACCGCC GAGCAACATC ATAAGCGGCA GCACCACCAG TAGCAACAAC
    AACAATACGA CGACCAGCAG CATCAGCAGC AGCGACAACA ACAACCTGCC CGCCACCAGC
    AACATCGAGC CATGCGCCAT CAGCAATCAG ACGAACTCGA GCGGCTGCGG CACGGACATA
    TCCGACACCA CGTCCGACGA ACTGGCCGGC GACGATATGG ACGTCAGGCG GCGGGATCGG
    GATCGGGACC GGGATCTGCT GGGCGCCAGC GATTCGGAGC TGAGTCGCTG CTATGTGAGC
    GAGACGAGTT CGCTGACCGG TGGCCTAACG GCCGGCGGCT ACGAGAATCC CACTTTCGCG
    CACTTTGCGG CCAATGCCAA TCGGGAGGAC GCTGTTTCGC TGGCCTCCGA CAGCGTTTGT
    CTCGGCCAGC CACGCCACGC CTATGTGGAT ACCTGCAGCG ACAGCGGCAG TGCCGTGGTG
    GTGATCTACG ACCACCAGAT TCCCAACACA CCCGACATTG AGTTCGTGAA GCAGAACTCT
    GAGATTGTAG TGCTGCGGAC TAAGGATCCG CAGCCCCACG CGCTGCAGCT GCACGAGATG
    CGCGAGCTGC AGCAGTTGCC CGCCAATTTG GCCGGTTCGC CGGACTCCTC GCCGGACTCT
    GCCGGTGGCC AGGCACCGCC AACAGCAACT GTGGCGCCCG CCAAGCAGCG ACTCTCCTCG
    TTTCGCGCCA CCAGTGAGCA GCAGTTGCAG CTCCTCGGAC GCGGTAGCCC GCAAAGAGGT
    AAAACACCCA GTGAGCAGGC GGTACAAAGC AGGCCACAGG ACCAGCATTT TCCACAGACA
    CAACAGCAGG ATATTGATGG CAGTAGTCCA CCAGTAGAAC TTGCAAGGCG CCAGCTGCCC
    CCCAAGCCCA CCAGTTTGAG TATTTTTAAC GGCCCCGTGC CCACTGCGGG CGATAGGCCT
    GTCGTGCCGC GAAAGTCGGA CTTTAAGGCT GATCTAGATG CTAAAATACG CAGGCAAAAG
    CAGAAGGTTA AACAGCAGTT GCAGCAGCAG CAGCAGCAAC AGCAGCAAGA GCAGCAAGAA
    ACGCAGCAGC AGCAGCAAGC ACCACAAGAA CAGCAACACT CACCACAGTC GCCCCAAACC
    AGAAACTGTA ATGTCACTAA TCAACAAGCC GCCAATATTA CTGCATCAGC ATCTGCATTT
    GCAACCGCAA CAGCATCCAC AGACCCGTAC CCGAATCCAA ATCATAGAAT GCCAAACCAA
    AATCAGACAG CCACATCCAA TCACACGCAG TGCAAGACGC CCACAATGGC ATTGTCACCG
    TCATCACCTC GCGGCCATTT GCCATTATCA TCGTCATCGC TATCGTCATT ACCATTACCA
    GCCACCACTT CATCACCATC AAATGCCCGG CCATCGATGT TGCCCGCCAG TGACCGACCA
    CCCGCCCATC CATATGTGTG CTCCAATGCC CCAGCCAATC CCCACCACGC CAATAGCATT
    TCCAATGCCA ATGCCCATCT CAAGCCGTGC ATTACGCCCC GGCCGGCTTC TTTGTCGGGA
    GGAGCAGCCG GCGGTGGCTC CACGCGCATC GGGCGTCGAT CGTCCATTAA TCAGTCCAAG
    CCACCGCCGC CAGTCAGACG CAGTTCGTCG GTGACCCCCA GTCCCAATGC CTCGGTCGGG
    CTGCAGCAGC AACCACAGCA CGCGACTCTG TCGCAGCAGA ATCACCAACT AAGCAGCTCC
    AGCGAGCACT TACCGCCGCC ACCGCCATTC ATGCTGGACG CCATGCCCCA GATTCCCAGC
    TCAGCGCTGA AGGTATCGGA GACGGTAAGA GCCCTGGCAG CCATGCGGCA CCAGCCAGCA
    TCACCTGTGT CCCTCAGACG CATGCAGCAG CAACAGCAGC AGCAGCTTCA GCAGCAACAG
    CAGCAACAGG TGCAGCAGCA GCAACCCCTA TTGCAGTCTG CGCACAACTC CCCCCTCAAA
    GAGGACCTGA CCGTGTACTA CGACTCCTAC TTGGATCTGC ACGCCTATGC CCAGGCCTTG
    GCCAGCGGCC AACAGCCCGG CGGTCAGCAG ATGGCCAACC AGCAACGCTT TACCCTCCAG
    CAGCAACATC AGCATCTGCA TCAGCCACAG CAACCGCCTG TCTACCAAGT CGATGCCACG
    TTCCGCACCT CATCACCAGC CGCGGGCGGA GGGGGTGGCG GCGGCATATA CGCCCAGCCC
    AAACTGGTCA ACAGCATGTC CAGCTTCCGC ACCAGCAGCC CCAGTCCCAA CGGACATGCT
    CACCCACTGC CACCGACACA GCCAAAGGCG AATCCGAACC TAATTGCACA GCTCAATGCA
    CGACTCAGCG GCAAACAGCA GCAGCACCAA CAGCAGCAGC ATATCGAGGG GATCTACGGC
    AACCAGCAGG CGCCCGGAGG AGAGTCGATC TACATGCGGA GTGGCCTGTC CATGTCGCAG
    CCGCAACAGC AGCAACACTT TGACGGTAAA TCTGAACAAA TCCAGCTGCA GCACCAGCAA
    CAGCATAGAA TTTACGCTAG TTTCGGCACC TCATCATCAG CAATGTCATC CGCTCATGCG
    GCCAACAGCA GCACTAAACC GTCCATTCTA ACACCGACCA CCTCTTTCAA TGCATTGCCT
    CACTTCCCCC TGTCTTCATC CACATCATCG TTGCTCTCCA AAGTCAGCTC ATTCTCGAAC
    TCTTCATCCG CATCCCCACC GACAACGGCA GCGACCTCTG GCTCGGCCAG TTCGCATTAT
    CAGCCACCTC AGCCGCCGAA TGCAGCAGTT GCTAACAGCA AAGACATGGC CATCTACTCA
    AGTTCGTTTA CCAAAAATCC AGCAGCTGCG CAATCGCCGA ACATGAGACA GGCTCATTCC
    CATCAGCACC ACCAGCCGCC GCAGCAGCAG CACTACACCT GTCCGCCTCC TCTGGAGGAT
    CCCCCACCGC CGCCCATTTA CGCCGCCGGT GCATCGGCCA CGATGCCCAA GAAGATGGCC
    CGTCCGCCCA CTGGCCAGAA CGCGACTCAC TCGAGCGCCT ATGCAGCAGC CTCGTCTACG
    GCCACGCTGC CCAAGAATAT GATGCAGCAG CAGCAGCGGT TGCAGCACCA GCAGCAGTAT
    CAACAGCCGG CAGGCATGGG CATTGGCAAC GGCAATGGGC ACCTAGGTCA GCGTCCGCAG
    TTGCCGCTGC CCCAGCAGAA GCTTAGAGCT GCACAGCAGC AGCACTTGGC GGAGCAGCAG
    CATCAGCAAC AGCAAGAACA GCAGCAGCAC CACCAGCAGC ACCAACAACA CCAGCAACGC
    CAGCCGCCCA TTCCTTCACG CCACTCAAGT GTGCAGCAAA AGATATTCGT ATCAACGAAT
    CCATTCATAC AGACAACGGC CGTCAAGTTT CACTCGCCCT CGGCCTCGCC CACGTGCGGC
    TCGCCCGTTA CTGGGTCCTT GGCTAGCATT TATGCCACAA CCTCGCGTGG CGGCCACCAT
    CACCAGCAGC AGCAGGCTCA GCAGCAGCAG CACTACTATC GCGATGCTGC CGGGGGCAAC
    AGCAACGGCG GCGCTGCCTA CTATAACCAC AATGCCCATG CCCATTCCCA GGCACATCAT
    CCAAACTATG CCACAAGCAC AAATATCGAA AAGACTGGCA GTATTCGTGC CAAGACCAAG
    GCCGAATTCC TCGAGAATCT CAACGCGAAG CTGGCGAAGC AGGGAATGTC TGGACGAGCA
    TTTGCCGTGC GAAATCTCAT CAACAGCAAG GCCCTGCCGG ACCCTCGCAT CTGTCACGAA
    TCGCTGATGG ATCAAATCAA GCGCGGAGCC ACCTTAAAGC GTAACCAGAA GATCAACGAT
    CGCAGCGCTC CCAAGATACA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa11767
    Clone ID Related Accession (Same CDS sequence) NM_001103735.3
    Accession Version NM_001103735.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product missing-in-metastasis, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001103735.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GACTTCGAAT GCAACGAACC AGACGGCAAA TGTCTCCACC
    TGGCCCCCAC ATTCCCAGGA TGGCGTCGAC ACCCTGCCAC CGACCGCTGA CCGTCCGCAC
    ACGATTTCGA CGGCATACGA AAAGGGTCAC CAGCGTCCGC CGCTGACCGT CTACACGTTC
    CAAAACCCGG AGACCATTCA CGAGTCGGGC AGCTGCCTGA ACAACGGAAC AGCAGCCCCG
    AATGGACAGC CGTTATCCGG ACAAGCCACT CCGGCCACCC AGAAATCACC GGCTGCCTCA
    CTTAGCCGGC CTCCTTTGCC AGTTCGCTGC TCGTCGTTGG AGCGACCCCT TTCGGCCCAG
    AGTAACCACC GCCAGGGTAG CGGGAATAAC CTGCTGCAGC GCCAGTGCCC CTCGCCGATT
    CCGGCTCATA TCACGAAAGA GCTGTCCGCA GCGCATCATG CACAGCAGCA GCAGCAGCAG
    CAAAATCAGC AGCCCCAGAC GCCGCCCACC TATGTGAACA TGTCGGAGCT GGCCACCATG
    GCCGCTTTGA AGCAGACCAA TCAGCAGCAG AAGCCCTCTA CGCCGCCTCT GCAGCAGCAG
    AGCTCCATTG ACTCGACCAG CTCCCAGCAT TCCAACGACT CCACCGGCTC TCATCAGCTC
    CTCCAGCAGC AGCAGCAGCA TCATCAATCG CAGCAGAATC ACCACTCAGC CACTGCCACA
    CGCTCCCATT CCATATCCTC GACGGCCTCG TCACTTCACT CGCATCCGTC GATCGACTCC
    ACCGTCGCTT GCGGCTCGCT GGTGGGCCAG CACAACCACA GCACCAGCAC CAACACGAAC
    ACCACCTCGC CGTCCAGTGG CAGCTCCACG CCACAGAACC ATTACTCGCC CCTGCTAACC
    AACTCCCCCA CGTCCACTGC CGCAGGTACT CCCAGTGGCA GCAGCTTAGG TCCTGGGTCC
    GGTTTGGGAT TTGTCTACCA GGTCAGCTCC CCGACGCCTC CCTCCAGCGA GGTGCTGAAA
    ATCACCGAGC AAGCCGCTGC AGGACAGGAT CAGGGTCCAG CCAACAGCGT AGCGGACGAG
    ACGGATGAGC GATCAAGGGC CTCAGTCCTG CAGAAGGCTT CAATGTTCGA AAAGGCGGCA
    GCAGCGGCAG CGGTATCGCC CCCAGCTCCC ATGCAGATTG CATCCGGTTC CCCAGCTTCG
    GGAGGCGGAA CTCGACGATC CGAGGCGGAG CAGCAGGAAA TGGACAAGTC TTTCGAAGAT
    TCAATACAAG CACTAAATAA TTTAATTGGC GAACTAGACT CGTTCCAACG CGAGATCGAT
    GAGGGCAAGG TCAAACCGCC GAGCAACATC ATAAGCGGCA GCACCACCAG TAGCAACAAC
    AACAATACGA CGACCAGCAG CATCAGCAGC AGCGACAACA ACAACCTGCC CGCCACCAGC
    AACATCGAGC CATGCGCCAT CAGCAATCAG ACGAACTCGA GCGGCTGCGG CACGGACATA
    TCCGACACCA CGTCCGACGA ACTGGCCGGC GACGATATGG ACGTCAGGCG GCGGGATCGG
    GATCGGGACC GGGATCTGCT GGGCGCCAGC GATTCGGAGC TGAGTCGCTG CTATGTGAGC
    GAGACGAGTT CGCTGACCGG TGGCCTAACG GCCGGCGGCT ACGAGAATCC CACTTTCGCG
    CACTTTGCGG CCAATGCCAA TCGGGAGGAC GCTGTTTCGC TGGCCTCCGA CAGCGTTTGT
    CTCGGCCAGC CACGCCACGC CTATGTGGAT ACCTGCAGCG ACAGCGGCAG TGCCGTGGTG
    GTGATCTACG ACCACCAGAT TCCCAACACA CCCGACATTG AGTTCGTGAA GCAGAACTCT
    GAGATTGTAG TGCTGCGGAC TAAGGATCCG CAGCCCCACG CGCTGCAGCT GCACGAGATG
    CGCGAGCTGC AGCAGTTGCC CGCCAATTTG GCCGGTTCGC CGGACTCCTC GCCGGACTCT
    GCCGGTGGCC AGGCACCGCC AACAGCAACT GTGGCGCCCG CCAAGCAGCG ACTCTCCTCG
    TTTCGCGCCA CCAGTGAGCA GCAGTTGCAG CTCCTCGGAC GCGGTAGCCC GCAAAGAGGT
    AAAACACCCA GTGAGCAGGC GGTACAAAGC AGGCCACAGG ACCAGCATTT TCCACAGACA
    CAACAGCAGG ATATTGATGG CAGTAGTCCA CCAGTAGAAC TTGCAAGGCG CCAGCTGCCC
    CCCAAGCCCA CCAGTTTGAG TATTTTTAAC GGCCCCGTGC CCACTGCGGG CGATAGGCCT
    GTCGTGCCGC GAAAGTCGGA CTTTAAGGCT GATCTAGATG CTAAAATACG CAGGCAAAAG
    CAGAAGGTTA AACAGCAGTT GCAGCAGCAG CAGCAGCAAC AGCAGCAAGA GCAGCAAGAA
    ACGCAGCAGC AGCAGCAAGC ACCACAAGAA CAGCAACACT CACCACAGTC GCCCCAAACC
    AGAAACTGTA ATGTCACTAA TCAACAAGCC GCCAATATTA CTGCATCAGC ATCTGCATTT
    GCAACCGCAA CAGCATCCAC AGACCCGTAC CCGAATCCAA ATCATAGAAT GCCAAACCAA
    AATCAGACAG CCACATCCAA TCACACGCAG TGCAAGACGC CCACAATGGC ATTGTCACCG
    TCATCACCTC GCGGCCATTT GCCATTATCA TCGTCATCGC TATCGTCATT ACCATTACCA
    GCCACCACTT CATCACCATC AAATGCCCGG CCATCGATGT TGCCCGCCAG TGACCGACCA
    CCCGCCCATC CATATGTGTG CTCCAATGCC CCAGCCAATC CCCACCACGC CAATAGCATT
    TCCAATGCCA ATGCCCATCT CAAGCCGTGC ATTACGCCCC GGCCGGCTTC TTTGTCGGGA
    GGAGCAGCCG GCGGTGGCTC CACGCGCATC GGGCGTCGAT CGTCCATTAA TCAGTCCAAG
    CCACCGCCGC CAGTCAGACG CAGTTCGTCG GTGACCCCCA GTCCCAATGC CTCGGTCGGG
    CTGCAGCAGC AACCACAGCA CGCGACTCTG TCGCAGCAGA ATCACCAACT AAGCAGCTCC
    AGCGAGCACT TACCGCCGCC ACCGCCATTC ATGCTGGACG CCATGCCCCA GATTCCCAGC
    TCAGCGCTGA AGGTATCGGA GACGGTAAGA GCCCTGGCAG CCATGCGGCA CCAGCCAGCA
    TCACCTGTGT CCCTCAGACG CATGCAGCAG CAACAGCAGC AGCAGCTTCA GCAGCAACAG
    CAGCAACAGG TGCAGCAGCA GCAACCCCTA TTGCAGTCTG CGCACAACTC CCCCCTCAAA
    GAGGACCTGA CCGTGTACTA CGACTCCTAC TTGGATCTGC ACGCCTATGC CCAGGCCTTG
    GCCAGCGGCC AACAGCCCGG CGGTCAGCAG ATGGCCAACC AGCAACGCTT TACCCTCCAG
    CAGCAACATC AGCATCTGCA TCAGCCACAG CAACCGCCTG TCTACCAAGT CGATGCCACG
    TTCCGCACCT CATCACCAGC CGCGGGCGGA GGGGGTGGCG GCGGCATATA CGCCCAGCCC
    AAACTGGTCA ACAGCATGTC CAGCTTCCGC ACCAGCAGCC CCAGTCCCAA CGGACATGCT
    CACCCACTGC CACCGACACA GCCAAAGGCG AATCCGAACC TAATTGCACA GCTCAATGCA
    CGACTCAGCG GCAAACAGCA GCAGCACCAA CAGCAGCAGC ATATCGAGGG GATCTACGGC
    AACCAGCAGG CGCCCGGAGG AGAGTCGATC TACATGCGGA GTGGCCTGTC CATGTCGCAG
    CCGCAACAGC AGCAACACTT TGACGACTAT GCCACAAGCA CAAATATCGA AAAGACTGGC
    AGTATTCGTG CCAAGACCAA GGCCGAATTC CTCGAGAATC TCAACGCGAA GCTGGCGAAG
    CAGGGAATGT CTGGACGAGC ATTTGCCGTG CGAAATCTCA TCAACAGCAA GGCCCTGCCG
    GACCCTCGCA TCTGTCACGA ATCGCTGATG GATCAAATCA AGCGCGGAGC CACCTTAAAG
    CGTAACCAGA AGATCAACGA TCGCAGCGCT CCCAAGATAC ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001097205.1
    CDS890..5854
    Translation

    Target ORF information:

    RefSeq Version NM_001103735.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster missing-in-metastasis (mim), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103735.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GACTTCGAAT GCAACGAACC AGACGGCAAA TGTCTCCACC
    TGGCCCCCAC ATTCCCAGGA TGGCGTCGAC ACCCTGCCAC CGACCGCTGA CCGTCCGCAC
    ACGATTTCGA CGGCATACGA AAAGGGTCAC CAGCGTCCGC CGCTGACCGT CTACACGTTC
    CAAAACCCGG AGACCATTCA CGAGTCGGGC AGCTGCCTGA ACAACGGAAC AGCAGCCCCG
    AATGGACAGC CGTTATCCGG ACAAGCCACT CCGGCCACCC AGAAATCACC GGCTGCCTCA
    CTTAGCCGGC CTCCTTTGCC AGTTCGCTGC TCGTCGTTGG AGCGACCCCT TTCGGCCCAG
    AGTAACCACC GCCAGGGTAG CGGGAATAAC CTGCTGCAGC GCCAGTGCCC CTCGCCGATT
    CCGGCTCATA TCACGAAAGA GCTGTCCGCA GCGCATCATG CACAGCAGCA GCAGCAGCAG
    CAAAATCAGC AGCCCCAGAC GCCGCCCACC TATGTGAACA TGTCGGAGCT GGCCACCATG
    GCCGCTTTGA AGCAGACCAA TCAGCAGCAG AAGCCCTCTA CGCCGCCTCT GCAGCAGCAG
    AGCTCCATTG ACTCGACCAG CTCCCAGCAT TCCAACGACT CCACCGGCTC TCATCAGCTC
    CTCCAGCAGC AGCAGCAGCA TCATCAATCG CAGCAGAATC ACCACTCAGC CACTGCCACA
    CGCTCCCATT CCATATCCTC GACGGCCTCG TCACTTCACT CGCATCCGTC GATCGACTCC
    ACCGTCGCTT GCGGCTCGCT GGTGGGCCAG CACAACCACA GCACCAGCAC CAACACGAAC
    ACCACCTCGC CGTCCAGTGG CAGCTCCACG CCACAGAACC ATTACTCGCC CCTGCTAACC
    AACTCCCCCA CGTCCACTGC CGCAGGTACT CCCAGTGGCA GCAGCTTAGG TCCTGGGTCC
    GGTTTGGGAT TTGTCTACCA GGTCAGCTCC CCGACGCCTC CCTCCAGCGA GGTGCTGAAA
    ATCACCGAGC AAGCCGCTGC AGGACAGGAT CAGGGTCCAG CCAACAGCGT AGCGGACGAG
    ACGGATGAGC GATCAAGGGC CTCAGTCCTG CAGAAGGCTT CAATGTTCGA AAAGGCGGCA
    GCAGCGGCAG CGGTATCGCC CCCAGCTCCC ATGCAGATTG CATCCGGTTC CCCAGCTTCG
    GGAGGCGGAA CTCGACGATC CGAGGCGGAG CAGCAGGAAA TGGACAAGTC TTTCGAAGAT
    TCAATACAAG CACTAAATAA TTTAATTGGC GAACTAGACT CGTTCCAACG CGAGATCGAT
    GAGGGCAAGG TCAAACCGCC GAGCAACATC ATAAGCGGCA GCACCACCAG TAGCAACAAC
    AACAATACGA CGACCAGCAG CATCAGCAGC AGCGACAACA ACAACCTGCC CGCCACCAGC
    AACATCGAGC CATGCGCCAT CAGCAATCAG ACGAACTCGA GCGGCTGCGG CACGGACATA
    TCCGACACCA CGTCCGACGA ACTGGCCGGC GACGATATGG ACGTCAGGCG GCGGGATCGG
    GATCGGGACC GGGATCTGCT GGGCGCCAGC GATTCGGAGC TGAGTCGCTG CTATGTGAGC
    GAGACGAGTT CGCTGACCGG TGGCCTAACG GCCGGCGGCT ACGAGAATCC CACTTTCGCG
    CACTTTGCGG CCAATGCCAA TCGGGAGGAC GCTGTTTCGC TGGCCTCCGA CAGCGTTTGT
    CTCGGCCAGC CACGCCACGC CTATGTGGAT ACCTGCAGCG ACAGCGGCAG TGCCGTGGTG
    GTGATCTACG ACCACCAGAT TCCCAACACA CCCGACATTG AGTTCGTGAA GCAGAACTCT
    GAGATTGTAG TGCTGCGGAC TAAGGATCCG CAGCCCCACG CGCTGCAGCT GCACGAGATG
    CGCGAGCTGC AGCAGTTGCC CGCCAATTTG GCCGGTTCGC CGGACTCCTC GCCGGACTCT
    GCCGGTGGCC AGGCACCGCC AACAGCAACT GTGGCGCCCG CCAAGCAGCG ACTCTCCTCG
    TTTCGCGCCA CCAGTGAGCA GCAGTTGCAG CTCCTCGGAC GCGGTAGCCC GCAAAGAGGT
    AAAACACCCA GTGAGCAGGC GGTACAAAGC AGGCCACAGG ACCAGCATTT TCCACAGACA
    CAACAGCAGG ATATTGATGG CAGTAGTCCA CCAGTAGAAC TTGCAAGGCG CCAGCTGCCC
    CCCAAGCCCA CCAGTTTGAG TATTTTTAAC GGCCCCGTGC CCACTGCGGG CGATAGGCCT
    GTCGTGCCGC GAAAGTCGGA CTTTAAGGCT GATCTAGATG CTAAAATACG CAGGCAAAAG
    CAGAAGGTTA AACAGCAGTT GCAGCAGCAG CAGCAGCAAC AGCAGCAAGA GCAGCAAGAA
    ACGCAGCAGC AGCAGCAAGC ACCACAAGAA CAGCAACACT CACCACAGTC GCCCCAAACC
    AGAAACTGTA ATGTCACTAA TCAACAAGCC GCCAATATTA CTGCATCAGC ATCTGCATTT
    GCAACCGCAA CAGCATCCAC AGACCCGTAC CCGAATCCAA ATCATAGAAT GCCAAACCAA
    AATCAGACAG CCACATCCAA TCACACGCAG TGCAAGACGC CCACAATGGC ATTGTCACCG
    TCATCACCTC GCGGCCATTT GCCATTATCA TCGTCATCGC TATCGTCATT ACCATTACCA
    GCCACCACTT CATCACCATC AAATGCCCGG CCATCGATGT TGCCCGCCAG TGACCGACCA
    CCCGCCCATC CATATGTGTG CTCCAATGCC CCAGCCAATC CCCACCACGC CAATAGCATT
    TCCAATGCCA ATGCCCATCT CAAGCCGTGC ATTACGCCCC GGCCGGCTTC TTTGTCGGGA
    GGAGCAGCCG GCGGTGGCTC CACGCGCATC GGGCGTCGAT CGTCCATTAA TCAGTCCAAG
    CCACCGCCGC CAGTCAGACG CAGTTCGTCG GTGACCCCCA GTCCCAATGC CTCGGTCGGG
    CTGCAGCAGC AACCACAGCA CGCGACTCTG TCGCAGCAGA ATCACCAACT AAGCAGCTCC
    AGCGAGCACT TACCGCCGCC ACCGCCATTC ATGCTGGACG CCATGCCCCA GATTCCCAGC
    TCAGCGCTGA AGGTATCGGA GACGGTAAGA GCCCTGGCAG CCATGCGGCA CCAGCCAGCA
    TCACCTGTGT CCCTCAGACG CATGCAGCAG CAACAGCAGC AGCAGCTTCA GCAGCAACAG
    CAGCAACAGG TGCAGCAGCA GCAACCCCTA TTGCAGTCTG CGCACAACTC CCCCCTCAAA
    GAGGACCTGA CCGTGTACTA CGACTCCTAC TTGGATCTGC ACGCCTATGC CCAGGCCTTG
    GCCAGCGGCC AACAGCCCGG CGGTCAGCAG ATGGCCAACC AGCAACGCTT TACCCTCCAG
    CAGCAACATC AGCATCTGCA TCAGCCACAG CAACCGCCTG TCTACCAAGT CGATGCCACG
    TTCCGCACCT CATCACCAGC CGCGGGCGGA GGGGGTGGCG GCGGCATATA CGCCCAGCCC
    AAACTGGTCA ACAGCATGTC CAGCTTCCGC ACCAGCAGCC CCAGTCCCAA CGGACATGCT
    CACCCACTGC CACCGACACA GCCAAAGGCG AATCCGAACC TAATTGCACA GCTCAATGCA
    CGACTCAGCG GCAAACAGCA GCAGCACCAA CAGCAGCAGC ATATCGAGGG GATCTACGGC
    AACCAGCAGG CGCCCGGAGG AGAGTCGATC TACATGCGGA GTGGCCTGTC CATGTCGCAG
    CCGCAACAGC AGCAACACTT TGACGACTAT GCCACAAGCA CAAATATCGA AAAGACTGGC
    AGTATTCGTG CCAAGACCAA GGCCGAATTC CTCGAGAATC TCAACGCGAA GCTGGCGAAG
    CAGGGAATGT CTGGACGAGC ATTTGCCGTG CGAAATCTCA TCAACAGCAA GGCCCTGCCG
    GACCCTCGCA TCTGTCACGA ATCGCTGATG GATCAAATCA AGCGCGGAGC CACCTTAAAG
    CGTAACCAGA AGATCAACGA TCGCAGCGCT CCCAAGATAC ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12117
    Clone ID Related Accession (Same CDS sequence) NM_001273819.1
    Accession Version NM_001273819.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product missing-in-metastasis, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG GTACTCCCAG TGGCAGCAGC
    TTAGGTCCTG GGTCCGGTTT GGGATTTGTC TACCAGGTCA GCTCCCCGAC GCCTCCCTCC
    AGCGAGGTGC TGAAAATCAC CGAGCAAGCC GCTGCAGGAC AGGATCAGGG TCCAGCCAAC
    AGCGTAGCGG ACGAGACGGA TGAGCGATCA AGGGCCTCAG TCCTGCAGAA GGCTTCAATG
    TTCGAAAAGG CGGCAGCAGC GGCAGCGGTA TCGCCCCCAG CTCCCATGCA GATTGCATCC
    GGTTCCCCAG CTTCGGGAGG CGGAACTCGA CGATCCGAGG CGGAGCAGCA GGAAATGGAC
    AAGTCTTTCG AAGATTCAAT ACAAGCACTA AATAATTTAA TTGGCGAACT AGACTCGTTC
    CAACGCGAGA TCGATGAGGG CAAGGTCAAA CCGCCGAGCA ACATCATAAG CGGCAGCACC
    ACCAGTAGCA ACAACAACAA TACGACGACC AGCAGCATCA GCAGCAGCGA CAACAACAAC
    CTGCCCGCCA CCAGCAACAT CGAGCCATGC GCCATCAGCA ATCAGACGAA CTCGAGCGGC
    TGCGGCACGG ACATATCCGA CACCACGTCC GACGAACTGG CCGGCGACGA TATGGACGTC
    AGGCGGCGGG ATCGGGATCG GGACCGGGAT CTGCTGGGCG CCAGCGATTC GGAGCTGAGT
    CGCTGCTATG TGAGCGAGAC GAGTTCGCTG ACCGGTGGCC TAACGGCCGG CGGCTACGAG
    AATCCCACTT TCGCGCACTT TGCGGCCAAT GCCAATCGGG AGGACGCTGT TTCGCTGGCC
    TCCGACAGCG TTTGTCTCGG CCAGCCACGC CACGCCTATG TGGATACCTG CAGCGACAGC
    GGCAGTGCCG TGGTGGTGAT CTACGACCAC CAGATTCCCA ACACACCCGA CATTGAGTTC
    GTGAAGCAGA ACTCTGAGAT TGTAGTGCTG CGGACTAAGG ATCCGCAGCC CCACGCGCTG
    CAGCTGCACG AGATGCGCGA GCTGCAGCAG TTGCCCGCCA ATTTGGCCGG TTCGCCGGAC
    TCCTCGCCGG ACTCTGCCGG TGGCCAGGCA CCGCCAACAG CAACTGTGGC GCCCGCCAAG
    CAGCGACTCT CCTCGTTTCG CGCCACCAGT GAGCAGCAGT TGCAGCTCCT CGGACGCGGT
    AGCCCGCAAA GAGGTAAAAC ACCCAGTGAG CAGGCGGTAC AAAGCAGGCC ACAGGACCAG
    CATTTTCCAC AGACACAACA GCAGGATATT GATGGCAGTA GTCCACCAGT AGAACTTGCA
    AGGCGCCAGC TGCCCCCCAA GCCCACCAGT TTGAGTATTT TTAACGGCCC CGTGCCCACT
    GCGGGCGATA GGCCTGTCGT GCCGCGAAAG TCGGACTTTA AGGCTGATCT AGATGCTAAA
    ATACGCAGGC AAAAGCAGAA GGTTAAACAG CAGTTGCAGC AGCAGCAGCA GCAACAGCAG
    CAAGAGCAGC AAGAAACGCA GCAGCAGCAG CAAGCACCAC AAGAACAGCA ACACTCACCA
    CAGTCGCCCC AAACCAGAAA CTGTAATGTC ACTAATCAAC AAGCCGCCAA TATTACTGCA
    TCAGCATCTG CATTTGCAAC CGCAACAGCA TCCACAGACC CGTACCCGAA TCCAAATCAT
    AGAATGCCAA ACCAAAATCA GACAGCCACA TCCAATCACA CGCAGTGCAA GACGCCCACA
    ATGGCATTGT CACCGTCATC ACCTCGCGGC CATTTGCCAT TATCATCGTC ATCGCTATCG
    TCATTACCAT TACCAGCCAC CACTTCATCA CCATCAAATG CCCGGCCATC GATGTTGCCC
    GCCAGTGACC GACCACCCGC CCATCCATAT GTGTGCTCCA ATGCCCCAGC CAATCCCCAC
    CACGCCAATA GCATTTCCAA TGCCAATGCC CATCTCAAGC CGTGCATTAC GCCCCGGCCG
    GCTTCTTTGT CGGGAGGAGC AGCCGGCGGT GGCTCCACGC GCATCGGGCG TCGATCGTCC
    ATTAATCAGT CCAAGCCACC GCCGCCAGTC AGACGCAGTT CGTCGGTGAC CCCCAGTCCC
    AATGCCTCGG TCGGGCTGCA GCAGCAACCA CAGCACGCGA CTCTGTCGCA GCAGAATCAC
    CAACTAAGCA GCTCCAGCGA GCACTTACCG CCGCCACCGC CATTCATGCT GGACGCCATG
    CCCCAGATTC CCAGCTCAGC GCTGAAGGTA TCGGAGACGG TAAGAGCCCT GGCAGCCATG
    CGGCACCAGC CAGCATCACC TGTGTCCCTC AGACGCATGC AGCAGCAACA GCAGCAGCAG
    CTTCAGCAGC AACAGCAGCA ACAGTCTGCG CACAACTCCC CCCTCAAAGA GGACCTGACC
    GTGTACTACG ACTCCTACTT GGATCTGCAC GCCTATGCCC AGGCCTTGGC CAGCGGCCAA
    CAGCCCGGCG GTCAGCAGAT GGCCAACCAG CAACGCTTTA CCCTCCAGCA GCAACATCAG
    CATCTGCATC AGCCACAGCA ACCGCCTGTC TACCAAGTCG ATGCCACGTT CCGCACCTCA
    TCACCAGCCG CGGGCGGAGG GGGTGGCGGC GGCATATACG CCCAGCCCAA ACTGGTCAAC
    AGCATGTCCA GCTTCCGCAC CAGCAGCCCC AGTCCCAACG GACATGCTCA CCCACTGCCA
    CCGACACAGC CAAAGGCGAA TCCGAACCTA ATTGCACAGC TCAATGCACG ACTCAGCGGC
    AAACAGCAGC AGCACCAACA GCAGCAGCAT ATCGAGGGGA TCTACGGCAA CCAGCAGGCG
    CCCGGAGGAG AGTCGATCTA CATGCGGAGT GGCCTGTCCA TGTCGCAGCC GCAACAGCAG
    CAACACTTTG ACGCGACCTC TGGCTCGGCC AGTTCGCATT ATCAGCCACC TCAGCCGCCG
    AATGCAGCAG TTGCTAACAG CAAAGACATG GCCATCTACT CAAGTTCGTT TACCAAAAAT
    CCAGCAGCTG CGCAATCGCC GAACATGAGA CAGGCTCATT CCCATCAGCA CCACCAGCCG
    CCGCAGCAGC AGCACTACAC CTGTCCGCCT CCTCTGGAGG ATCCCCCACC GCCGCCCATT
    TACGCCGCCG GTGCATCGGC CACGATGCCC AAGAAGATGG CCCGTCCGCC CACTGGCCAG
    AACGCGACTC ACTCGAGCGC CTATGCAGCA GCCTCGTCTA CGGCCACGCT GCCCAAGAAT
    ATGATGCAGC AGCAGCAGCG GTTGCAGCAC CAGCAGCAGT ATCAACAGCC GGCAGGCATG
    GGCATTGGCA ACGGCAATGG GCACCTAGGT CAGCGTCCGC AGTTGCCGCT GCCCCAGCAG
    AAGCTTAGAG CTGCACAGCA GCAGCACTTG GCGGAGCAGC AGCATCAGCA ACAGCAAGAA
    CAGCAGCAGC ACCACCAGCA GCACCAACAA CACCAGCAAC GCCAGCCGCC CATTCCTTCA
    CGCCACTCAA GTGTGCAGCA AAAGATATTC GTATCAACGA ATCCATTCAT ACAGACAACG
    GCCGTCAAGT TTCACTCGCC CTCGGCCTCG CCCACGTGCG GCTCGCCCGT TACTGGGTCC
    TTGGCTAGCA TTTATGCCAC AACCTCGCGT GGCGGCCACC ATCACCAGCA GCAGCAGGCT
    CAGCAGCAGC AGCACTACTA TCGCGATGCT GCCGGGGGCA ACAGCAACGG CGGCGCTGCC
    TACTATAACC ACAATGCCCA TGCCCATTCC CAGGCACATC ATCCAAACTA TGCCACAAGC
    ACAAATATCG AAAAGACTGG CAGTATTCGT GCCAAGACCA AGGCCGAATT CCTCGAGAAT
    CTCAACGCGA AGCTGGCGAA GCAGGGAATG TCTGGACGAG CATTTGCCGT GCGAAATCTC
    ATCAACAGCA AGGCCCTGAT GTATCAAAAT CCGCAAAAAC TATCGCGACC CAGTGCGCAA
    TACCGTAGAC CACCCACCTA TCCCAACACC AGCACAACCA CCAATGCCAC TTGCGAAGAT
    CAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260748.1
    CDS890..6298
    Translation

    Target ORF information:

    RefSeq Version NM_001273819.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster missing-in-metastasis (mim), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273819.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG GTACTCCCAG TGGCAGCAGC
    TTAGGTCCTG GGTCCGGTTT GGGATTTGTC TACCAGGTCA GCTCCCCGAC GCCTCCCTCC
    AGCGAGGTGC TGAAAATCAC CGAGCAAGCC GCTGCAGGAC AGGATCAGGG TCCAGCCAAC
    AGCGTAGCGG ACGAGACGGA TGAGCGATCA AGGGCCTCAG TCCTGCAGAA GGCTTCAATG
    TTCGAAAAGG CGGCAGCAGC GGCAGCGGTA TCGCCCCCAG CTCCCATGCA GATTGCATCC
    GGTTCCCCAG CTTCGGGAGG CGGAACTCGA CGATCCGAGG CGGAGCAGCA GGAAATGGAC
    AAGTCTTTCG AAGATTCAAT ACAAGCACTA AATAATTTAA TTGGCGAACT AGACTCGTTC
    CAACGCGAGA TCGATGAGGG CAAGGTCAAA CCGCCGAGCA ACATCATAAG CGGCAGCACC
    ACCAGTAGCA ACAACAACAA TACGACGACC AGCAGCATCA GCAGCAGCGA CAACAACAAC
    CTGCCCGCCA CCAGCAACAT CGAGCCATGC GCCATCAGCA ATCAGACGAA CTCGAGCGGC
    TGCGGCACGG ACATATCCGA CACCACGTCC GACGAACTGG CCGGCGACGA TATGGACGTC
    AGGCGGCGGG ATCGGGATCG GGACCGGGAT CTGCTGGGCG CCAGCGATTC GGAGCTGAGT
    CGCTGCTATG TGAGCGAGAC GAGTTCGCTG ACCGGTGGCC TAACGGCCGG CGGCTACGAG
    AATCCCACTT TCGCGCACTT TGCGGCCAAT GCCAATCGGG AGGACGCTGT TTCGCTGGCC
    TCCGACAGCG TTTGTCTCGG CCAGCCACGC CACGCCTATG TGGATACCTG CAGCGACAGC
    GGCAGTGCCG TGGTGGTGAT CTACGACCAC CAGATTCCCA ACACACCCGA CATTGAGTTC
    GTGAAGCAGA ACTCTGAGAT TGTAGTGCTG CGGACTAAGG ATCCGCAGCC CCACGCGCTG
    CAGCTGCACG AGATGCGCGA GCTGCAGCAG TTGCCCGCCA ATTTGGCCGG TTCGCCGGAC
    TCCTCGCCGG ACTCTGCCGG TGGCCAGGCA CCGCCAACAG CAACTGTGGC GCCCGCCAAG
    CAGCGACTCT CCTCGTTTCG CGCCACCAGT GAGCAGCAGT TGCAGCTCCT CGGACGCGGT
    AGCCCGCAAA GAGGTAAAAC ACCCAGTGAG CAGGCGGTAC AAAGCAGGCC ACAGGACCAG
    CATTTTCCAC AGACACAACA GCAGGATATT GATGGCAGTA GTCCACCAGT AGAACTTGCA
    AGGCGCCAGC TGCCCCCCAA GCCCACCAGT TTGAGTATTT TTAACGGCCC CGTGCCCACT
    GCGGGCGATA GGCCTGTCGT GCCGCGAAAG TCGGACTTTA AGGCTGATCT AGATGCTAAA
    ATACGCAGGC AAAAGCAGAA GGTTAAACAG CAGTTGCAGC AGCAGCAGCA GCAACAGCAG
    CAAGAGCAGC AAGAAACGCA GCAGCAGCAG CAAGCACCAC AAGAACAGCA ACACTCACCA
    CAGTCGCCCC AAACCAGAAA CTGTAATGTC ACTAATCAAC AAGCCGCCAA TATTACTGCA
    TCAGCATCTG CATTTGCAAC CGCAACAGCA TCCACAGACC CGTACCCGAA TCCAAATCAT
    AGAATGCCAA ACCAAAATCA GACAGCCACA TCCAATCACA CGCAGTGCAA GACGCCCACA
    ATGGCATTGT CACCGTCATC ACCTCGCGGC CATTTGCCAT TATCATCGTC ATCGCTATCG
    TCATTACCAT TACCAGCCAC CACTTCATCA CCATCAAATG CCCGGCCATC GATGTTGCCC
    GCCAGTGACC GACCACCCGC CCATCCATAT GTGTGCTCCA ATGCCCCAGC CAATCCCCAC
    CACGCCAATA GCATTTCCAA TGCCAATGCC CATCTCAAGC CGTGCATTAC GCCCCGGCCG
    GCTTCTTTGT CGGGAGGAGC AGCCGGCGGT GGCTCCACGC GCATCGGGCG TCGATCGTCC
    ATTAATCAGT CCAAGCCACC GCCGCCAGTC AGACGCAGTT CGTCGGTGAC CCCCAGTCCC
    AATGCCTCGG TCGGGCTGCA GCAGCAACCA CAGCACGCGA CTCTGTCGCA GCAGAATCAC
    CAACTAAGCA GCTCCAGCGA GCACTTACCG CCGCCACCGC CATTCATGCT GGACGCCATG
    CCCCAGATTC CCAGCTCAGC GCTGAAGGTA TCGGAGACGG TAAGAGCCCT GGCAGCCATG
    CGGCACCAGC CAGCATCACC TGTGTCCCTC AGACGCATGC AGCAGCAACA GCAGCAGCAG
    CTTCAGCAGC AACAGCAGCA ACAGTCTGCG CACAACTCCC CCCTCAAAGA GGACCTGACC
    GTGTACTACG ACTCCTACTT GGATCTGCAC GCCTATGCCC AGGCCTTGGC CAGCGGCCAA
    CAGCCCGGCG GTCAGCAGAT GGCCAACCAG CAACGCTTTA CCCTCCAGCA GCAACATCAG
    CATCTGCATC AGCCACAGCA ACCGCCTGTC TACCAAGTCG ATGCCACGTT CCGCACCTCA
    TCACCAGCCG CGGGCGGAGG GGGTGGCGGC GGCATATACG CCCAGCCCAA ACTGGTCAAC
    AGCATGTCCA GCTTCCGCAC CAGCAGCCCC AGTCCCAACG GACATGCTCA CCCACTGCCA
    CCGACACAGC CAAAGGCGAA TCCGAACCTA ATTGCACAGC TCAATGCACG ACTCAGCGGC
    AAACAGCAGC AGCACCAACA GCAGCAGCAT ATCGAGGGGA TCTACGGCAA CCAGCAGGCG
    CCCGGAGGAG AGTCGATCTA CATGCGGAGT GGCCTGTCCA TGTCGCAGCC GCAACAGCAG
    CAACACTTTG ACGCGACCTC TGGCTCGGCC AGTTCGCATT ATCAGCCACC TCAGCCGCCG
    AATGCAGCAG TTGCTAACAG CAAAGACATG GCCATCTACT CAAGTTCGTT TACCAAAAAT
    CCAGCAGCTG CGCAATCGCC GAACATGAGA CAGGCTCATT CCCATCAGCA CCACCAGCCG
    CCGCAGCAGC AGCACTACAC CTGTCCGCCT CCTCTGGAGG ATCCCCCACC GCCGCCCATT
    TACGCCGCCG GTGCATCGGC CACGATGCCC AAGAAGATGG CCCGTCCGCC CACTGGCCAG
    AACGCGACTC ACTCGAGCGC CTATGCAGCA GCCTCGTCTA CGGCCACGCT GCCCAAGAAT
    ATGATGCAGC AGCAGCAGCG GTTGCAGCAC CAGCAGCAGT ATCAACAGCC GGCAGGCATG
    GGCATTGGCA ACGGCAATGG GCACCTAGGT CAGCGTCCGC AGTTGCCGCT GCCCCAGCAG
    AAGCTTAGAG CTGCACAGCA GCAGCACTTG GCGGAGCAGC AGCATCAGCA ACAGCAAGAA
    CAGCAGCAGC ACCACCAGCA GCACCAACAA CACCAGCAAC GCCAGCCGCC CATTCCTTCA
    CGCCACTCAA GTGTGCAGCA AAAGATATTC GTATCAACGA ATCCATTCAT ACAGACAACG
    GCCGTCAAGT TTCACTCGCC CTCGGCCTCG CCCACGTGCG GCTCGCCCGT TACTGGGTCC
    TTGGCTAGCA TTTATGCCAC AACCTCGCGT GGCGGCCACC ATCACCAGCA GCAGCAGGCT
    CAGCAGCAGC AGCACTACTA TCGCGATGCT GCCGGGGGCA ACAGCAACGG CGGCGCTGCC
    TACTATAACC ACAATGCCCA TGCCCATTCC CAGGCACATC ATCCAAACTA TGCCACAAGC
    ACAAATATCG AAAAGACTGG CAGTATTCGT GCCAAGACCA AGGCCGAATT CCTCGAGAAT
    CTCAACGCGA AGCTGGCGAA GCAGGGAATG TCTGGACGAG CATTTGCCGT GCGAAATCTC
    ATCAACAGCA AGGCCCTGAT GTATCAAAAT CCGCAAAAAC TATCGCGACC CAGTGCGCAA
    TACCGTAGAC CACCCACCTA TCCCAACACC AGCACAACCA CCAATGCCAC TTGCGAAGAT
    CAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12118
    Clone ID Related Accession (Same CDS sequence) NM_001201941.2
    Accession Version NM_001201941.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6030bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product missing-in-metastasis, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001201941.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GACTTCGAAT GCAACGAACC AGACGGCAAA TGTCTCCACC
    TGGCCCCCAC ATTCCCAGGA TGGCGTCGAC ACCCTGCCAC CGACCGCTGA CCGTCCGCAC
    ACGATTTCGA CGGCATACGA AAAGGGTCAC CAGCGTCCGC CGCTGACCGT CTACACGTTC
    CAAAACCCGG AGACCATTCA CGAGTCGGGC AGCTGCCTGA ACAACGGAAC AGCAGCCCCG
    AATGGACAGC CGTTATCCGG ACAAGCCACT CCGGCCACCC AGAAATCACC GGCTGCCTCA
    CTTAGCCGGC CTCCTTTGCC AGTTCGCTGC TCGTCGTTGG AGCGACCCCT TTCGGCCCAG
    AGTAACCACC GCCAGGGTAG CGGGAATAAC CTGCTGCAGC GCCAGTGCCC CTCGCCGATT
    CCGGCTCATA TCACGAAAGA GCTGTCCGCA GCGCATCATG CACAGCAGCA GCAGCAGCAG
    CAAAATCAGC AGCCCCAGAC GCCGCCCACC TATGTGAACA TGTCGGAGCT GGCCACCATG
    GCCGCTTTGA AGCAGACCAA TCAGCAGCAG AAGCCCTCTA CGCCGCCTCT GCAGCAGCAG
    AGCTCCATTG ACTCGACCAG CTCCCAGCAT TCCAACGACT CCACCGGCTC TCATCAGCTC
    CTCCAGCAGC AGCAGCAGCA TCATCAATCG CAGCAGAATC ACCACTCAGC CACTGCCACA
    CGCTCCCATT CCATATCCTC GACGGCCTCG TCACTTCACT CGCATCCGTC GATCGACTCC
    ACCGTCGCTT GCGGCTCGCT GGTGGGCCAG CACAACCACA GCACCAGCAC CAACACGAAC
    ACCACCTCGC CGTCCAGTGG CAGCTCCACG CCACAGAACC ATTACTCGCC CCTGCTAACC
    AACTCCCCCA CGTCCACTGC CGCAGGTACT CCCAGTGGCA GCAGCTTAGG TCCTGGGTCC
    GGTTTGGGAT TTGTCTACCA GGTCAGCTCC CCGACGCCTC CCTCCAGCGA GGTGCTGAAA
    ATCACCGAGC AAGCCGCTGC AGGACAGGAT CAGGGTCCAG CCAACAGCGT AGCGGACGAG
    ACGGATGAGC GATCAAGGGC CTCAGTCCTG CAGAAGGCTT CAATGTTCGA AAAGGCGGCA
    GCAGCGGCAG CGGTATCGCC CCCAGCTCCC ATGCAGATTG CATCCGGTTC CCCAGCTTCG
    GGAGGCGGAA CTCGACGATC CGAGGCGGAG CAGCAGGAAA TGGACTCGTT CCAACGCGAG
    ATCGATGAGG GCAAGGTCAA ACCGCCGAGC AACATCATAA GCGGCAGCAC CACCAGTAGC
    AACAACAACA ATACGACGAC CAGCAGCATC AGCAGCAGCG ACAACAACAA CCTGCCCGCC
    ACCAGCAACA TCGAGCCATG CGCCATCAGC AATCAGACGA ACTCGAGCGG CTGCGGCACG
    GACATATCCG ACACCACGTC CGACGAACTG GCCGGCGACG ATATGGACGT CAGGCGGCGG
    GATCGGGATC GGGACCGGGA TCTGCTGGGC GCCAGCGATT CGGAGCTGAG TCGCTGCTAT
    GTGAGCGAGA CGAGTTCGCT GACCGGTGGC CTAACGGCCG GCGGCTACGA GAATCCCACT
    TTCGCGCACT TTGCGGCCAA TGCCAATCGG GAGGACGCTG TTTCGCTGGC CTCCGACAGC
    GTTTGTCTCG GCCAGCCACG CCACGCCTAT GTGGATACCT GCAGCGACAG CGGCAGTGCC
    GTGGTGGTGA TCTACGACCA CCAGATTCCC AACACACCCG ACATTGAGTT CGTGAAGCAG
    AACTCTGAGA TTGTAGTGCT GCGGACTAAG GATCCGCAGC CCCACGCGCT GCAGCTGCAC
    GAGATGCGCG AGCTGCAGCA GTTGCCCGCC AATTTGGCCG GTTCGCCGGA CTCCTCGCCG
    GACTCTGCCG GTGGCCAGGC ACCGCCAACA GCAACTGTGG CGCCCGCCAA GCAGCGACTC
    TCCTCGTTTC GCGCCACCAG TGAGCAGCAG TTGCAGCTCC TCGGACGCGG TAGCCCGCAA
    AGAGGTAAAA CACCCAGTGA GCAGGCGGTA CAAAGCAGGC CACAGGACCA GCATTTTCCA
    CAGACACAAC AGCAGGATAT TGATGGCAGT AGTCCACCAG TAGAACTTGC AAGGCGCCAG
    CTGCCCCCCA AGCCCACCAG TTTGAGTATT TTTAACGGCC CCGTGCCCAC TGCGGGCGAT
    AGGCCTGTCG TGCCGCGAAA GTCGGACTTT AAGGCTGATC TAGATGCTAA AATACGCAGG
    CAAAAGCAGA AGGTTAAACA GCAGTTGCAG CAGCAGCAGC AGCAACAGCA GCAAGAGCAG
    CAAGAAACGC AGCAGCAGCA GCAAGCACCA CAAGAACAGC AACACTCACC ACAGTCGCCC
    CAAACCAGAA ACTGTAATGT CACTAATCAA CAAGCCGCCA ATATTACTGC ATCAGCATCT
    GCATTTGCAA CCGCAACAGC ATCCACAGAC CCGTACCCGA ATCCAAATCA TAGAATGCCA
    AACCAAAATC AGACAGCCAC ATCCAATCAC ACGCAGTGCA AGACGCCCAC AATGGCATTG
    TCACCGTCAT CACCTCGCGG CCATTTGCCA TTATCATCGT CATCGCTATC GTCATTACCA
    TTACCAGCCA CCACTTCATC ACCATCAAAT GCCCGGCCAT CGATGTTGCC CGCCAGTGAC
    CGACCACCCG CCCATCCATA TGTGTGCTCC AATGCCCCAG CCAATCCCCA CCACGCCAAT
    AGCATTTCCA ATGCCAATGC CCATCTCAAG CCGTGCATTA CGCCCCGGCC GGCTTCTTTG
    TCGGGAGGAG CAGCCGGCGG TGGCTCCACG CGCATCGGGC GTCGATCGTC CATTAATCAG
    TCCAAGCCAC CGCCGCCAGT CAGACGCAGT TCGTCGGTGA CCCCCAGTCC CAATGCCTCG
    GTCGGGCTGC AGCAGCAACC ACAGCACGCG ACTCTGTCGC AGCAGAATCA CCAACTAAGC
    AGCTCCAGCG AGCACTTACC GCCGCCACCG CCATTCATGC TGGACGCCAT GCCCCAGATT
    CCCAGCTCAG CGCTGAAGGT ATCGGAGACG GTAAGAGCCC TGGCAGCCAT GCGGCACCAG
    CCAGCATCAC CTGTGTCCCT CAGACGCATG CAGCAGCAAC AGCAGCAGCA GCTTCAGCAG
    CAACAGCAGC AACAGGTGCA GCAGCAGCAA CCCCTATTGC AGTCTGCGCA CAACTCCCCC
    CTCAAAGAGG ACCTGACCGT GTACTACGAC TCCTACTTGG ATCTGCACGC CTATGCCCAG
    GCCTTGGCCA GCGGCCAACA GCCCGGCGGT CAGCAGATGG CCAACCAGCA ACGCTTTACC
    CTCCAGCAGC AACATCAGCA TCTGCATCAG CCACAGCAAC CGCCTGTCTA CCAAGTCGAT
    GCCACGTTCC GCACCTCATC ACCAGCCGCG GGCGGAGGGG GTGGCGGCGG CATATACGCC
    CAGCCCAAAC TGGTCAACAG CATGTCCAGC TTCCGCACCA GCAGCCCCAG TCCCAACGGA
    CATGCTCACC CACTGCCACC GACACAGCCA AAGGCGAATC CGAACCTAAT TGCACAGCTC
    AATGCACGAC TCAGCGGCAA ACAGCAGCAG CACCAACAGC AGCAGCATAT CGAGGGGATC
    TACGGCAACC AGCAGGCGCC CGGAGGAGAG TCGATCTACA TGCGGAGTGG CCTGTCCATG
    TCGCAGCCGC AACAGCAGCA ACACTTTGAC GGTAAATCTG AACAAATCCA GCTGCAGCAC
    CAGCAACAGC ATAGAATTTA CGCTAGTTTC GGCACCTCAT CATCAGCAAT GTCATCCGCT
    CATGCGGCCA ACAGCAGCAC TAAACCGTCC ATTCTAACAC CGACCACCTC TTTCAATGCA
    TTGCCTCACT TCCCCCTGTC TTCATCCACA TCATCGTTGC TCTCCAAAGT CAGCTCATTC
    TCGAACTCTT CATCCGCATC CCCACCGACA ACGGCAGCGA CCTCTGGCTC GGCCAGTTCG
    CATTATCAGC CACCTCAGCC GCCGAATGCA GCAGTTGCTA ACAGCAAAGA CATGGCCATC
    TACTCAAGTT CGTTTACCAA AAATCCAGCA GCTGCGCAAT CGCCGAACAT GAGACAGGCT
    CATTCCCATC AGCACCACCA GCCGCCGCAG CAGCAGCACT ACACCTGTCC GCCTCCTCTG
    GAGGATCCCC CACCGCCGCC CATTTACGCC GCCGGTGCAT CGGCCACGAT GCCCAAGAAG
    ATGGCCCGTC CGCCCACTGG CCAGAACGCG ACTCACTCGA GCGCCTATGC AGCAGCCTCG
    TCTACGGCCA CGCTGCCCAA GAATATGATG CAGCAGCAGC AGCGGTTGCA GCACCAGCAG
    CAGTATCAAC AGCCGGCAGG CATGGGCATT GGCAACGGCA ATGGGCACCT AGGTCAGCGT
    CCGCAGTTGC CGCTGCCCCA GCAGAAGCTT AGAGCTGCAC AGCAGCAGCA CTTGGCGGAG
    CAGCAGCATC AGCAACAGCA AGAACAGCAG CAGCACCACC AGCAGCACCA ACAACACCAG
    CAACGCCAGC CGCCCATTCC TTCACGCCAC TCAAGTGTGC AGCAAAAGAT ATTCGTATCA
    ACGAATCCAT TCATACAGAC AACGGCCGTC AAGTTTCACT CGCCCTCGGC CTCGCCCACG
    TGCGGCTCGC CCGTTACTGG GTCCTTGGCT AGCATTTATG CCACAACCTC GCGTGGCGGC
    CACCATCACC AGCAGCAGCA GGCTCAGCAG CAGCAGCACT ACTATCGCGA TGCTGCCGGG
    GGCAACAGCA ACGGCGGCGC TGCCTACTAT AACCACAATG CCCATGCCCA TTCCCAGGCA
    CATCATCCAA ACTATGCCAC AAGCACAAAT ATCGAAAAGA CTGGCAGTAT TCGTGCCAAG
    ACCAAGGCCG AATTCCTCGA GAATCTCAAC GCGAAGCTGG CGAAGCAGGG AATGTCTGGA
    CGAGCATTTG CCGTGCGAAA TCTCATCAAC AGCAAGGCCC TGCCGGACCC TCGCATCTGT
    CACGAATCGC TGATGGATCA AATCAAGCGC GGAGCCACCT TAAAGCGTAA CCAGAAGATC
    AACGATCGCA GCGCTCCCAA GATACATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188870.1
    CDS890..6919
    Translation

    Target ORF information:

    RefSeq Version NM_001201941.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster missing-in-metastasis (mim), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001201941.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GACTTCGAAT GCAACGAACC AGACGGCAAA TGTCTCCACC
    TGGCCCCCAC ATTCCCAGGA TGGCGTCGAC ACCCTGCCAC CGACCGCTGA CCGTCCGCAC
    ACGATTTCGA CGGCATACGA AAAGGGTCAC CAGCGTCCGC CGCTGACCGT CTACACGTTC
    CAAAACCCGG AGACCATTCA CGAGTCGGGC AGCTGCCTGA ACAACGGAAC AGCAGCCCCG
    AATGGACAGC CGTTATCCGG ACAAGCCACT CCGGCCACCC AGAAATCACC GGCTGCCTCA
    CTTAGCCGGC CTCCTTTGCC AGTTCGCTGC TCGTCGTTGG AGCGACCCCT TTCGGCCCAG
    AGTAACCACC GCCAGGGTAG CGGGAATAAC CTGCTGCAGC GCCAGTGCCC CTCGCCGATT
    CCGGCTCATA TCACGAAAGA GCTGTCCGCA GCGCATCATG CACAGCAGCA GCAGCAGCAG
    CAAAATCAGC AGCCCCAGAC GCCGCCCACC TATGTGAACA TGTCGGAGCT GGCCACCATG
    GCCGCTTTGA AGCAGACCAA TCAGCAGCAG AAGCCCTCTA CGCCGCCTCT GCAGCAGCAG
    AGCTCCATTG ACTCGACCAG CTCCCAGCAT TCCAACGACT CCACCGGCTC TCATCAGCTC
    CTCCAGCAGC AGCAGCAGCA TCATCAATCG CAGCAGAATC ACCACTCAGC CACTGCCACA
    CGCTCCCATT CCATATCCTC GACGGCCTCG TCACTTCACT CGCATCCGTC GATCGACTCC
    ACCGTCGCTT GCGGCTCGCT GGTGGGCCAG CACAACCACA GCACCAGCAC CAACACGAAC
    ACCACCTCGC CGTCCAGTGG CAGCTCCACG CCACAGAACC ATTACTCGCC CCTGCTAACC
    AACTCCCCCA CGTCCACTGC CGCAGGTACT CCCAGTGGCA GCAGCTTAGG TCCTGGGTCC
    GGTTTGGGAT TTGTCTACCA GGTCAGCTCC CCGACGCCTC CCTCCAGCGA GGTGCTGAAA
    ATCACCGAGC AAGCCGCTGC AGGACAGGAT CAGGGTCCAG CCAACAGCGT AGCGGACGAG
    ACGGATGAGC GATCAAGGGC CTCAGTCCTG CAGAAGGCTT CAATGTTCGA AAAGGCGGCA
    GCAGCGGCAG CGGTATCGCC CCCAGCTCCC ATGCAGATTG CATCCGGTTC CCCAGCTTCG
    GGAGGCGGAA CTCGACGATC CGAGGCGGAG CAGCAGGAAA TGGACTCGTT CCAACGCGAG
    ATCGATGAGG GCAAGGTCAA ACCGCCGAGC AACATCATAA GCGGCAGCAC CACCAGTAGC
    AACAACAACA ATACGACGAC CAGCAGCATC AGCAGCAGCG ACAACAACAA CCTGCCCGCC
    ACCAGCAACA TCGAGCCATG CGCCATCAGC AATCAGACGA ACTCGAGCGG CTGCGGCACG
    GACATATCCG ACACCACGTC CGACGAACTG GCCGGCGACG ATATGGACGT CAGGCGGCGG
    GATCGGGATC GGGACCGGGA TCTGCTGGGC GCCAGCGATT CGGAGCTGAG TCGCTGCTAT
    GTGAGCGAGA CGAGTTCGCT GACCGGTGGC CTAACGGCCG GCGGCTACGA GAATCCCACT
    TTCGCGCACT TTGCGGCCAA TGCCAATCGG GAGGACGCTG TTTCGCTGGC CTCCGACAGC
    GTTTGTCTCG GCCAGCCACG CCACGCCTAT GTGGATACCT GCAGCGACAG CGGCAGTGCC
    GTGGTGGTGA TCTACGACCA CCAGATTCCC AACACACCCG ACATTGAGTT CGTGAAGCAG
    AACTCTGAGA TTGTAGTGCT GCGGACTAAG GATCCGCAGC CCCACGCGCT GCAGCTGCAC
    GAGATGCGCG AGCTGCAGCA GTTGCCCGCC AATTTGGCCG GTTCGCCGGA CTCCTCGCCG
    GACTCTGCCG GTGGCCAGGC ACCGCCAACA GCAACTGTGG CGCCCGCCAA GCAGCGACTC
    TCCTCGTTTC GCGCCACCAG TGAGCAGCAG TTGCAGCTCC TCGGACGCGG TAGCCCGCAA
    AGAGGTAAAA CACCCAGTGA GCAGGCGGTA CAAAGCAGGC CACAGGACCA GCATTTTCCA
    CAGACACAAC AGCAGGATAT TGATGGCAGT AGTCCACCAG TAGAACTTGC AAGGCGCCAG
    CTGCCCCCCA AGCCCACCAG TTTGAGTATT TTTAACGGCC CCGTGCCCAC TGCGGGCGAT
    AGGCCTGTCG TGCCGCGAAA GTCGGACTTT AAGGCTGATC TAGATGCTAA AATACGCAGG
    CAAAAGCAGA AGGTTAAACA GCAGTTGCAG CAGCAGCAGC AGCAACAGCA GCAAGAGCAG
    CAAGAAACGC AGCAGCAGCA GCAAGCACCA CAAGAACAGC AACACTCACC ACAGTCGCCC
    CAAACCAGAA ACTGTAATGT CACTAATCAA CAAGCCGCCA ATATTACTGC ATCAGCATCT
    GCATTTGCAA CCGCAACAGC ATCCACAGAC CCGTACCCGA ATCCAAATCA TAGAATGCCA
    AACCAAAATC AGACAGCCAC ATCCAATCAC ACGCAGTGCA AGACGCCCAC AATGGCATTG
    TCACCGTCAT CACCTCGCGG CCATTTGCCA TTATCATCGT CATCGCTATC GTCATTACCA
    TTACCAGCCA CCACTTCATC ACCATCAAAT GCCCGGCCAT CGATGTTGCC CGCCAGTGAC
    CGACCACCCG CCCATCCATA TGTGTGCTCC AATGCCCCAG CCAATCCCCA CCACGCCAAT
    AGCATTTCCA ATGCCAATGC CCATCTCAAG CCGTGCATTA CGCCCCGGCC GGCTTCTTTG
    TCGGGAGGAG CAGCCGGCGG TGGCTCCACG CGCATCGGGC GTCGATCGTC CATTAATCAG
    TCCAAGCCAC CGCCGCCAGT CAGACGCAGT TCGTCGGTGA CCCCCAGTCC CAATGCCTCG
    GTCGGGCTGC AGCAGCAACC ACAGCACGCG ACTCTGTCGC AGCAGAATCA CCAACTAAGC
    AGCTCCAGCG AGCACTTACC GCCGCCACCG CCATTCATGC TGGACGCCAT GCCCCAGATT
    CCCAGCTCAG CGCTGAAGGT ATCGGAGACG GTAAGAGCCC TGGCAGCCAT GCGGCACCAG
    CCAGCATCAC CTGTGTCCCT CAGACGCATG CAGCAGCAAC AGCAGCAGCA GCTTCAGCAG
    CAACAGCAGC AACAGGTGCA GCAGCAGCAA CCCCTATTGC AGTCTGCGCA CAACTCCCCC
    CTCAAAGAGG ACCTGACCGT GTACTACGAC TCCTACTTGG ATCTGCACGC CTATGCCCAG
    GCCTTGGCCA GCGGCCAACA GCCCGGCGGT CAGCAGATGG CCAACCAGCA ACGCTTTACC
    CTCCAGCAGC AACATCAGCA TCTGCATCAG CCACAGCAAC CGCCTGTCTA CCAAGTCGAT
    GCCACGTTCC GCACCTCATC ACCAGCCGCG GGCGGAGGGG GTGGCGGCGG CATATACGCC
    CAGCCCAAAC TGGTCAACAG CATGTCCAGC TTCCGCACCA GCAGCCCCAG TCCCAACGGA
    CATGCTCACC CACTGCCACC GACACAGCCA AAGGCGAATC CGAACCTAAT TGCACAGCTC
    AATGCACGAC TCAGCGGCAA ACAGCAGCAG CACCAACAGC AGCAGCATAT CGAGGGGATC
    TACGGCAACC AGCAGGCGCC CGGAGGAGAG TCGATCTACA TGCGGAGTGG CCTGTCCATG
    TCGCAGCCGC AACAGCAGCA ACACTTTGAC GGTAAATCTG AACAAATCCA GCTGCAGCAC
    CAGCAACAGC ATAGAATTTA CGCTAGTTTC GGCACCTCAT CATCAGCAAT GTCATCCGCT
    CATGCGGCCA ACAGCAGCAC TAAACCGTCC ATTCTAACAC CGACCACCTC TTTCAATGCA
    TTGCCTCACT TCCCCCTGTC TTCATCCACA TCATCGTTGC TCTCCAAAGT CAGCTCATTC
    TCGAACTCTT CATCCGCATC CCCACCGACA ACGGCAGCGA CCTCTGGCTC GGCCAGTTCG
    CATTATCAGC CACCTCAGCC GCCGAATGCA GCAGTTGCTA ACAGCAAAGA CATGGCCATC
    TACTCAAGTT CGTTTACCAA AAATCCAGCA GCTGCGCAAT CGCCGAACAT GAGACAGGCT
    CATTCCCATC AGCACCACCA GCCGCCGCAG CAGCAGCACT ACACCTGTCC GCCTCCTCTG
    GAGGATCCCC CACCGCCGCC CATTTACGCC GCCGGTGCAT CGGCCACGAT GCCCAAGAAG
    ATGGCCCGTC CGCCCACTGG CCAGAACGCG ACTCACTCGA GCGCCTATGC AGCAGCCTCG
    TCTACGGCCA CGCTGCCCAA GAATATGATG CAGCAGCAGC AGCGGTTGCA GCACCAGCAG
    CAGTATCAAC AGCCGGCAGG CATGGGCATT GGCAACGGCA ATGGGCACCT AGGTCAGCGT
    CCGCAGTTGC CGCTGCCCCA GCAGAAGCTT AGAGCTGCAC AGCAGCAGCA CTTGGCGGAG
    CAGCAGCATC AGCAACAGCA AGAACAGCAG CAGCACCACC AGCAGCACCA ACAACACCAG
    CAACGCCAGC CGCCCATTCC TTCACGCCAC TCAAGTGTGC AGCAAAAGAT ATTCGTATCA
    ACGAATCCAT TCATACAGAC AACGGCCGTC AAGTTTCACT CGCCCTCGGC CTCGCCCACG
    TGCGGCTCGC CCGTTACTGG GTCCTTGGCT AGCATTTATG CCACAACCTC GCGTGGCGGC
    CACCATCACC AGCAGCAGCA GGCTCAGCAG CAGCAGCACT ACTATCGCGA TGCTGCCGGG
    GGCAACAGCA ACGGCGGCGC TGCCTACTAT AACCACAATG CCCATGCCCA TTCCCAGGCA
    CATCATCCAA ACTATGCCAC AAGCACAAAT ATCGAAAAGA CTGGCAGTAT TCGTGCCAAG
    ACCAAGGCCG AATTCCTCGA GAATCTCAAC GCGAAGCTGG CGAAGCAGGG AATGTCTGGA
    CGAGCATTTG CCGTGCGAAA TCTCATCAAC AGCAAGGCCC TGCCGGACCC TCGCATCTGT
    CACGAATCGC TGATGGATCA AATCAAGCGC GGAGCCACCT TAAAGCGTAA CCAGAAGATC
    AACGATCGCA GCGCTCCCAA GATACATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12119
    Clone ID Related Accession (Same CDS sequence) NM_001169597.2
    Accession Version NM_001169597.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2853bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product missing-in-metastasis, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169597.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GACTTCGAAT GCAACGAACC AGACGGCAAA TGTCTCCACC
    TGGCCCCCAC ATTCCCAGGA TGGCGTCGAC ACCCTGCCAC CGACCGCTGA CCGTCCGCAC
    ACGATTTCGA CGGCATACGA AAAGGGTCAC CAGCGTCCGC CGCTGACCGT CTACACGTTC
    CAAAACCCGG AGACCATTCA CGAGTCGGGC AGCTGCCTGA ACAACGGAAC AGCAGCCCCG
    AATGGACAGC CGTTATCCGG ACAAGCCACT CCGGCCACCC AGAAATCACC GGCTGCCTCA
    CTTAGCCGGC CTCCTTTGCC AGTTCGCTGC TCGTCGTTGG AGCGACCCCT TTCGGCCCAG
    AGTAACCACC GCCAGGGTAG CGGGAATAAC CTGCTGCAGC GCCAGTGCCC CTCGCCGATT
    CCGGCTCATA TCACGAAAGA GCTGTCCGCA GCGCATCATG CACAGCAGCA GCAGCAGCAG
    CAAAATCAGC AGCCCCAGAC GCCGCCCACC TATGTGAACA TGTCGGAGCT GGCCACCATG
    GCCGCTTTGA AGCAGACCAA TCAGCAGCAG AAGCCCTCTA CGCCGCCTCT GCAGCAGCAG
    AGCTCCATTG ACTCGACCAG CTCCCAGCAT TCCAACGACT CCACCGGCTC TCATCAGCTC
    CTCCAGCAGC AGCAGCAGCA TCATCAATCG CAGCAGAATC ACCACTCAGC CACTGCCACA
    CGCTCCCATT CCATATCCTC GACGGCCTCG TCACTTCACT CGCATCCGTC GATCGACTCC
    ACCGTCGCTT GCGGCTCGCT GGTGGGCCAG CACAACCACA GCACCAGCAC CAACACGAAC
    ACCACCTCGC CGTCCAGTGG CAGCTCCACG CCACAGAACC ATTACTCGCC CCTGCTAACC
    AACTCCCCCA CGTCCACTGC CGCAGGTACT CCCAGTGGCA GCAGCTTAGG TCCTGGGTCC
    GGTTTGGGAT TTGTCTACCA GGTCAGCTCC CCGACGCCTC CCTCCAGCGA GGTGCTGAAA
    ATCACCGAGC AAGCCGCTGC AGGACAGGAT CAGGGTCCAG CCAACAGCGT AGCGGACGAG
    ACGGATGAGC GATCAAGGGC CTCAGTCCTG CAGAAGGCTT CAATGTTCGA AAAGGCGGCA
    GCAGCGGCAG CGGTATCGCC CCCAGCTCCC ATGCAGATTG CATCCGGTTC CCCAGCTTCG
    GGAGGCGGAA CTCGACGATC CGAGGCGGAG CAGCAGGAAA TGGGAGGAGC AGCCGGCGGT
    GGCTCCACGC GCATCGGGCG TCGATCGTCC ATTAATCAGT CCAAGCCACC GCCGCCAGTC
    AGACGCAGTT CGTCGGTGAC CCCCAGTCCC AATGCCTCGG TCGGGACGTT CCGCACCTCA
    TCACCAGCCG CGGGCGGAGG GGGTGGCGGC GGCATATACG CCCAGCCCAA ACTGGTCAAC
    AGCATGTCCA GCTTCCGCAC CAGCAGCCCC AGTCCCAACG GACATGCTCA CCCACTGCCA
    CCGACACAGC CAAAGGCGAA TCCGAACCTA ATTGCACAGC TCAATGCACG ACTCAGCGGC
    AAACAGCAGC AGCACCAACA GCAGCAGCAT ATCGAGGGGA TCTACGGCAA CCAGCAGGCG
    CCCGGAGGAG AGTCGATCTA CATGCGGAGT GGCCTGTCCA TGTCGCAGCC GCAACAGCAG
    CAACACTTTG ACGACTATGC CACAAGCACA AATATCGAAA AGACTGGCAG TATTCGTGCC
    AAGACCAAGG CCGAATTCCT CGAGAATCTC AACGCGAAGC TGGCGAAGCA GGGAATGTCT
    GGACGAGCAT TTGCCGTGCG AAATCTCATC AACAGCAAGG CCCTGCCGGA CCCTCGCATC
    TGTCACGAAT CGCTGATGGA TCAAATCAAG CGCGGAGCCA CCTTAAAGCG TAACCAGAAG
    ATCAACGATC GCAGCGCTCC CAAGATACAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163068.1
    CDS890..3742
    Translation

    Target ORF information:

    RefSeq Version NM_001169597.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster missing-in-metastasis (mim), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169597.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GACTTCGAAT GCAACGAACC AGACGGCAAA TGTCTCCACC
    TGGCCCCCAC ATTCCCAGGA TGGCGTCGAC ACCCTGCCAC CGACCGCTGA CCGTCCGCAC
    ACGATTTCGA CGGCATACGA AAAGGGTCAC CAGCGTCCGC CGCTGACCGT CTACACGTTC
    CAAAACCCGG AGACCATTCA CGAGTCGGGC AGCTGCCTGA ACAACGGAAC AGCAGCCCCG
    AATGGACAGC CGTTATCCGG ACAAGCCACT CCGGCCACCC AGAAATCACC GGCTGCCTCA
    CTTAGCCGGC CTCCTTTGCC AGTTCGCTGC TCGTCGTTGG AGCGACCCCT TTCGGCCCAG
    AGTAACCACC GCCAGGGTAG CGGGAATAAC CTGCTGCAGC GCCAGTGCCC CTCGCCGATT
    CCGGCTCATA TCACGAAAGA GCTGTCCGCA GCGCATCATG CACAGCAGCA GCAGCAGCAG
    CAAAATCAGC AGCCCCAGAC GCCGCCCACC TATGTGAACA TGTCGGAGCT GGCCACCATG
    GCCGCTTTGA AGCAGACCAA TCAGCAGCAG AAGCCCTCTA CGCCGCCTCT GCAGCAGCAG
    AGCTCCATTG ACTCGACCAG CTCCCAGCAT TCCAACGACT CCACCGGCTC TCATCAGCTC
    CTCCAGCAGC AGCAGCAGCA TCATCAATCG CAGCAGAATC ACCACTCAGC CACTGCCACA
    CGCTCCCATT CCATATCCTC GACGGCCTCG TCACTTCACT CGCATCCGTC GATCGACTCC
    ACCGTCGCTT GCGGCTCGCT GGTGGGCCAG CACAACCACA GCACCAGCAC CAACACGAAC
    ACCACCTCGC CGTCCAGTGG CAGCTCCACG CCACAGAACC ATTACTCGCC CCTGCTAACC
    AACTCCCCCA CGTCCACTGC CGCAGGTACT CCCAGTGGCA GCAGCTTAGG TCCTGGGTCC
    GGTTTGGGAT TTGTCTACCA GGTCAGCTCC CCGACGCCTC CCTCCAGCGA GGTGCTGAAA
    ATCACCGAGC AAGCCGCTGC AGGACAGGAT CAGGGTCCAG CCAACAGCGT AGCGGACGAG
    ACGGATGAGC GATCAAGGGC CTCAGTCCTG CAGAAGGCTT CAATGTTCGA AAAGGCGGCA
    GCAGCGGCAG CGGTATCGCC CCCAGCTCCC ATGCAGATTG CATCCGGTTC CCCAGCTTCG
    GGAGGCGGAA CTCGACGATC CGAGGCGGAG CAGCAGGAAA TGGGAGGAGC AGCCGGCGGT
    GGCTCCACGC GCATCGGGCG TCGATCGTCC ATTAATCAGT CCAAGCCACC GCCGCCAGTC
    AGACGCAGTT CGTCGGTGAC CCCCAGTCCC AATGCCTCGG TCGGGACGTT CCGCACCTCA
    TCACCAGCCG CGGGCGGAGG GGGTGGCGGC GGCATATACG CCCAGCCCAA ACTGGTCAAC
    AGCATGTCCA GCTTCCGCAC CAGCAGCCCC AGTCCCAACG GACATGCTCA CCCACTGCCA
    CCGACACAGC CAAAGGCGAA TCCGAACCTA ATTGCACAGC TCAATGCACG ACTCAGCGGC
    AAACAGCAGC AGCACCAACA GCAGCAGCAT ATCGAGGGGA TCTACGGCAA CCAGCAGGCG
    CCCGGAGGAG AGTCGATCTA CATGCGGAGT GGCCTGTCCA TGTCGCAGCC GCAACAGCAG
    CAACACTTTG ACGACTATGC CACAAGCACA AATATCGAAA AGACTGGCAG TATTCGTGCC
    AAGACCAAGG CCGAATTCCT CGAGAATCTC AACGCGAAGC TGGCGAAGCA GGGAATGTCT
    GGACGAGCAT TTGCCGTGCG AAATCTCATC AACAGCAAGG CCCTGCCGGA CCCTCGCATC
    TGTCACGAAT CGCTGATGGA TCAAATCAAG CGCGGAGCCA CCTTAAAGCG TAACCAGAAG
    ATCAACGATC GCAGCGCTCC CAAGATACAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12120
    Clone ID Related Accession (Same CDS sequence) NM_001201940.3
    Accession Version NM_001201940.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6087bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product missing-in-metastasis, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001201940.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GACTTCGAAT GCAACGAACC AGACGGCAAA TGTCTCCACC
    TGGCCCCCAC ATTCCCAGGA TGGCGTCGAC ACCCTGCCAC CGACCGCTGA CCGTCCGCAC
    ACGATTTCGA CGGCATACGA AAAGGGTCAC CAGCGTCCGC CGCTGACCGT CTACACGTTC
    CAAAACCCGG AGACCATTCA CGAGTCGGGC AGCTGCCTGA ACAACGGAAC AGCAGCCCCG
    AATGGACAGC CGTTATCCGG ACAAGCCACT CCGGCCACCC AGAAATCACC GGCTGCCTCA
    CTTAGCCGGC CTCCTTTGCC AGTTCGCTGC TCGTCGTTGG AGCGACCCCT TTCGGCCCAG
    AGTAACCACC GCCAGGGTAG CGGGAATAAC CTGCTGCAGC GCCAGTGCCC CTCGCCGATT
    CCGGCTCATA TCACGAAAGA GCTGTCCGCA GCGCATCATG CACAGCAGCA GCAGCAGCAG
    CAAAATCAGC AGCCCCAGAC GCCGCCCACC TATGTGAACA TGTCGGAGCT GGCCACCATG
    GCCGCTTTGA AGCAGACCAA TCAGCAGCAG AAGCCCTCTA CGCCGCCTCT GCAGCAGCAG
    AGCTCCATTG ACTCGACCAG CTCCCAGCAT TCCAACGACT CCACCGGCTC TCATCAGCTC
    CTCCAGCAGC AGCAGCAGCA TCATCAATCG CAGCAGAATC ACCACTCAGC CACTGCCACA
    CGCTCCCATT CCATATCCTC GACGGCCTCG TCACTTCACT CGCATCCGTC GATCGACTCC
    ACCGTCGCTT GCGGCTCGCT GGTGGGCCAG CACAACCACA GCACCAGCAC CAACACGAAC
    ACCACCTCGC CGTCCAGTGG CAGCTCCACG CCACAGAACC ATTACTCGCC CCTGCTAACC
    AACTCCCCCA CGTCCACTGC CGCAGGTACT CCCAGTGGCA GCAGCTTAGG TCCTGGGTCC
    GGTTTGGGAT TTGTCTACCA GGTCAGCTCC CCGACGCCTC CCTCCAGCGA GGTGCTGAAA
    ATCACCGAGC AAGCCGCTGC AGGACAGGAT CAGGGTCCAG CCAACAGCGT AGCGGACGAG
    ACGGATGAGC GATCAAGGGC CTCAGTCCTG CAGAAGGCTT CAATGTTCGA AAAGGCGGCA
    GCAGCGGCAG CGGTATCGCC CCCAGCTCCC ATGCAGATTG CATCCGGTTC CCCAGCTTCG
    GGAGGCGGAA CTCGACGATC CGAGGCGGAG CAGCAGGAAA TGGACAAGTC TTTCGAAGAT
    TCAATACAAG CACTAAATAA TTTAATTGGC GAACTAGACT CGTTCCAACG CGAGATCGAT
    GAGGGCAAGG TCAAACCGCC GAGCAACATC ATAAGCGGCA GCACCACCAG TAGCAACAAC
    AACAATACGA CGACCAGCAG CATCAGCAGC AGCGACAACA ACAACCTGCC CGCCACCAGC
    AACATCGAGC CATGCGCCAT CAGCAATCAG ACGAACTCGA GCGGCTGCGG CACGGACATA
    TCCGACACCA CGTCCGACGA ACTGGCCGGC GACGATATGG ACGTCAGGCG GCGGGATCGG
    GATCGGGACC GGGATCTGCT GGGCGCCAGC GATTCGGAGC TGAGTCGCTG CTATGTGAGC
    GAGACGAGTT CGCTGACCGG TGGCCTAACG GCCGGCGGCT ACGAGAATCC CACTTTCGCG
    CACTTTGCGG CCAATGCCAA TCGGGAGGAC GCTGTTTCGC TGGCCTCCGA CAGCGTTTGT
    CTCGGCCAGC CACGCCACGC CTATGTGGAT ACCTGCAGCG ACAGCGGCAG TGCCGTGGTG
    GTGATCTACG ACCACCAGAT TCCCAACACA CCCGACATTG AGTTCGTGAA GCAGAACTCT
    GAGATTGTAG TGCTGCGGAC TAAGGATCCG CAGCCCCACG CGCTGCAGCT GCACGAGATG
    CGCGAGCTGC AGCAGTTGCC CGCCAATTTG GCCGGTTCGC CGGACTCCTC GCCGGACTCT
    GCCGGTGGCC AGGCACCGCC AACAGCAACT GTGGCGCCCG CCAAGCAGCG ACTCTCCTCG
    TTTCGCGCCA CCAGTGAGCA GCAGTTGCAG CTCCTCGGAC GCGGTAGCCC GCAAAGAGGT
    AAAACACCCA GTGAGCAGGC GGTACAAAGC AGGCCACAGG ACCAGCATTT TCCACAGACA
    CAACAGCAGG ATATTGATGG CAGTAGTCCA CCAGTAGAAC TTGCAAGGCG CCAGCTGCCC
    CCCAAGCCCA CCAGTTTGAG TATTTTTAAC GGCCCCGTGC CCACTGCGGG CGATAGGCCT
    GTCGTGCCGC GAAAGTCGGA CTTTAAGGCT GATCTAGATG CTAAAATACG CAGGCAAAAG
    CAGAAGGTTA AACAGCAGTT GCAGCAGCAG CAGCAGCAAC AGCAGCAAGA GCAGCAAGAA
    ACGCAGCAGC AGCAGCAAGC ACCACAAGAA CAGCAACACT CACCACAGTC GCCCCAAACC
    AGAAACTGTA ATGTCACTAA TCAACAAGCC GCCAATATTA CTGCATCAGC ATCTGCATTT
    GCAACCGCAA CAGCATCCAC AGACCCGTAC CCGAATCCAA ATCATAGAAT GCCAAACCAA
    AATCAGACAG CCACATCCAA TCACACGCAG TGCAAGACGC CCACAATGGC ATTGTCACCG
    TCATCACCTC GCGGCCATTT GCCATTATCA TCGTCATCGC TATCGTCATT ACCATTACCA
    GCCACCACTT CATCACCATC AAATGCCCGG CCATCGATGT TGCCCGCCAG TGACCGACCA
    CCCGCCCATC CATATGTGTG CTCCAATGCC CCAGCCAATC CCCACCACGC CAATAGCATT
    TCCAATGCCA ATGCCCATCT CAAGCCGTGC ATTACGCCCC GGCCGGCTTC TTTGTCGGGA
    GGAGCAGCCG GCGGTGGCTC CACGCGCATC GGGCGTCGAT CGTCCATTAA TCAGTCCAAG
    CCACCGCCGC CAGTCAGACG CAGTTCGTCG GTGACCCCCA GTCCCAATGC CTCGGTCGGG
    CTGCAGCAGC AACCACAGCA CGCGACTCTG TCGCAGCAGA ATCACCAACT AAGCAGCTCC
    AGCGAGCACT TACCGCCGCC ACCGCCATTC ATGCTGGACG CCATGCCCCA GATTCCCAGC
    TCAGCGCTGA AGGTATCGGA GACGGTAAGA GCCCTGGCAG CCATGCGGCA CCAGCCAGCA
    TCACCTGTGT CCCTCAGACG CATGCAGCAG CAACAGCAGC AGCAGCTTCA GCAGCAACAG
    CAGCAACAGG TGCAGCAGCA GCAACCCCTA TTGCAGTCTG CGCACAACTC CCCCCTCAAA
    GAGGACCTGA CCGTGTACTA CGACTCCTAC TTGGATCTGC ACGCCTATGC CCAGGCCTTG
    GCCAGCGGCC AACAGCCCGG CGGTCAGCAG ATGGCCAACC AGCAACGCTT TACCCTCCAG
    CAGCAACATC AGCATCTGCA TCAGCCACAG CAACCGCCTG TCTACCAAGT CGATGCCACG
    TTCCGCACCT CATCACCAGC CGCGGGCGGA GGGGGTGGCG GCGGCATATA CGCCCAGCCC
    AAACTGGTCA ACAGCATGTC CAGCTTCCGC ACCAGCAGCC CCAGTCCCAA CGGACATGCT
    CACCCACTGC CACCGACACA GCCAAAGGCG AATCCGAACC TAATTGCACA GCTCAATGCA
    CGACTCAGCG GCAAACAGCA GCAGCACCAA CAGCAGCAGC ATATCGAGGG GATCTACGGC
    AACCAGCAGG CGCCCGGAGG AGAGTCGATC TACATGCGGA GTGGCCTGTC CATGTCGCAG
    CCGCAACAGC AGCAACACTT TGACGGTAAA TCTGAACAAA TCCAGCTGCA GCACCAGCAA
    CAGCATAGAA TTTACGCTAG TTTCGGCACC TCATCATCAG CAATGTCATC CGCTCATGCG
    GCCAACAGCA GCACTAAACC GTCCATTCTA ACACCGACCA CCTCTTTCAA TGCATTGCCT
    CACTTCCCCC TGTCTTCATC CACATCATCG TTGCTCTCCA AAGTCAGCTC ATTCTCGAAC
    TCTTCATCCG CATCCCCACC GACAACGGCA GCGACCTCTG GCTCGGCCAG TTCGCATTAT
    CAGCCACCTC AGCCGCCGAA TGCAGCAGTT GCTAACAGCA AAGACATGGC CATCTACTCA
    AGTTCGTTTA CCAAAAATCC AGCAGCTGCG CAATCGCCGA ACATGAGACA GGCTCATTCC
    CATCAGCACC ACCAGCCGCC GCAGCAGCAG CACTACACCT GTCCGCCTCC TCTGGAGGAT
    CCCCCACCGC CGCCCATTTA CGCCGCCGGT GCATCGGCCA CGATGCCCAA GAAGATGGCC
    CGTCCGCCCA CTGGCCAGAA CGCGACTCAC TCGAGCGCCT ATGCAGCAGC CTCGTCTACG
    GCCACGCTGC CCAAGAATAT GATGCAGCAG CAGCAGCGGT TGCAGCACCA GCAGCAGTAT
    CAACAGCCGG CAGGCATGGG CATTGGCAAC GGCAATGGGC ACCTAGGTCA GCGTCCGCAG
    TTGCCGCTGC CCCAGCAGAA GCTTAGAGCT GCACAGCAGC AGCACTTGGC GGAGCAGCAG
    CATCAGCAAC AGCAAGAACA GCAGCAGCAC CACCAGCAGC ACCAACAACA CCAGCAACGC
    CAGCCGCCCA TTCCTTCACG CCACTCAAGT GTGCAGCAAA AGATATTCGT ATCAACGAAT
    CCATTCATAC AGACAACGGC CGTCAAGTTT CACTCGCCCT CGGCCTCGCC CACGTGCGGC
    TCGCCCGTTA CTGGGTCCTT GGCTAGCATT TATGCCACAA CCTCGCGTGG CGGCCACCAT
    CACCAGCAGC AGCAGGCTCA GCAGCAGCAG CACTACTATC GCGATGCTGC CGGGGGCAAC
    AGCAACGGCG GCGCTGCCTA CTATAACCAC AATGCCCATG CCCATTCCCA GGCACATCAT
    CCAAACTATG CCACAAGCAC AAATATCGAA AAGACTGGCA GTATTCGTGC CAAGACCAAG
    GCCGAATTCC TCGAGAATCT CAACGCGAAG CTGGCGAAGC AGGGAATGTC TGGACGAGCA
    TTTGCCGTGC GAAATCTCAT CAACAGCAAG GCCCTGATGT ATCAAAATCC GCAAAAACTA
    TCGCGACCCA GTGCGCAATA CCGTAGACCA CCCACCTATC CCAACACCAG CACAACCACC
    AATGCCACTT GCGAAGATCA GTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188869.1
    CDS890..6976
    Translation

    Target ORF information:

    RefSeq Version NM_001201940.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster missing-in-metastasis (mim), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001201940.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GACTTCGAAT GCAACGAACC AGACGGCAAA TGTCTCCACC
    TGGCCCCCAC ATTCCCAGGA TGGCGTCGAC ACCCTGCCAC CGACCGCTGA CCGTCCGCAC
    ACGATTTCGA CGGCATACGA AAAGGGTCAC CAGCGTCCGC CGCTGACCGT CTACACGTTC
    CAAAACCCGG AGACCATTCA CGAGTCGGGC AGCTGCCTGA ACAACGGAAC AGCAGCCCCG
    AATGGACAGC CGTTATCCGG ACAAGCCACT CCGGCCACCC AGAAATCACC GGCTGCCTCA
    CTTAGCCGGC CTCCTTTGCC AGTTCGCTGC TCGTCGTTGG AGCGACCCCT TTCGGCCCAG
    AGTAACCACC GCCAGGGTAG CGGGAATAAC CTGCTGCAGC GCCAGTGCCC CTCGCCGATT
    CCGGCTCATA TCACGAAAGA GCTGTCCGCA GCGCATCATG CACAGCAGCA GCAGCAGCAG
    CAAAATCAGC AGCCCCAGAC GCCGCCCACC TATGTGAACA TGTCGGAGCT GGCCACCATG
    GCCGCTTTGA AGCAGACCAA TCAGCAGCAG AAGCCCTCTA CGCCGCCTCT GCAGCAGCAG
    AGCTCCATTG ACTCGACCAG CTCCCAGCAT TCCAACGACT CCACCGGCTC TCATCAGCTC
    CTCCAGCAGC AGCAGCAGCA TCATCAATCG CAGCAGAATC ACCACTCAGC CACTGCCACA
    CGCTCCCATT CCATATCCTC GACGGCCTCG TCACTTCACT CGCATCCGTC GATCGACTCC
    ACCGTCGCTT GCGGCTCGCT GGTGGGCCAG CACAACCACA GCACCAGCAC CAACACGAAC
    ACCACCTCGC CGTCCAGTGG CAGCTCCACG CCACAGAACC ATTACTCGCC CCTGCTAACC
    AACTCCCCCA CGTCCACTGC CGCAGGTACT CCCAGTGGCA GCAGCTTAGG TCCTGGGTCC
    GGTTTGGGAT TTGTCTACCA GGTCAGCTCC CCGACGCCTC CCTCCAGCGA GGTGCTGAAA
    ATCACCGAGC AAGCCGCTGC AGGACAGGAT CAGGGTCCAG CCAACAGCGT AGCGGACGAG
    ACGGATGAGC GATCAAGGGC CTCAGTCCTG CAGAAGGCTT CAATGTTCGA AAAGGCGGCA
    GCAGCGGCAG CGGTATCGCC CCCAGCTCCC ATGCAGATTG CATCCGGTTC CCCAGCTTCG
    GGAGGCGGAA CTCGACGATC CGAGGCGGAG CAGCAGGAAA TGGACAAGTC TTTCGAAGAT
    TCAATACAAG CACTAAATAA TTTAATTGGC GAACTAGACT CGTTCCAACG CGAGATCGAT
    GAGGGCAAGG TCAAACCGCC GAGCAACATC ATAAGCGGCA GCACCACCAG TAGCAACAAC
    AACAATACGA CGACCAGCAG CATCAGCAGC AGCGACAACA ACAACCTGCC CGCCACCAGC
    AACATCGAGC CATGCGCCAT CAGCAATCAG ACGAACTCGA GCGGCTGCGG CACGGACATA
    TCCGACACCA CGTCCGACGA ACTGGCCGGC GACGATATGG ACGTCAGGCG GCGGGATCGG
    GATCGGGACC GGGATCTGCT GGGCGCCAGC GATTCGGAGC TGAGTCGCTG CTATGTGAGC
    GAGACGAGTT CGCTGACCGG TGGCCTAACG GCCGGCGGCT ACGAGAATCC CACTTTCGCG
    CACTTTGCGG CCAATGCCAA TCGGGAGGAC GCTGTTTCGC TGGCCTCCGA CAGCGTTTGT
    CTCGGCCAGC CACGCCACGC CTATGTGGAT ACCTGCAGCG ACAGCGGCAG TGCCGTGGTG
    GTGATCTACG ACCACCAGAT TCCCAACACA CCCGACATTG AGTTCGTGAA GCAGAACTCT
    GAGATTGTAG TGCTGCGGAC TAAGGATCCG CAGCCCCACG CGCTGCAGCT GCACGAGATG
    CGCGAGCTGC AGCAGTTGCC CGCCAATTTG GCCGGTTCGC CGGACTCCTC GCCGGACTCT
    GCCGGTGGCC AGGCACCGCC AACAGCAACT GTGGCGCCCG CCAAGCAGCG ACTCTCCTCG
    TTTCGCGCCA CCAGTGAGCA GCAGTTGCAG CTCCTCGGAC GCGGTAGCCC GCAAAGAGGT
    AAAACACCCA GTGAGCAGGC GGTACAAAGC AGGCCACAGG ACCAGCATTT TCCACAGACA
    CAACAGCAGG ATATTGATGG CAGTAGTCCA CCAGTAGAAC TTGCAAGGCG CCAGCTGCCC
    CCCAAGCCCA CCAGTTTGAG TATTTTTAAC GGCCCCGTGC CCACTGCGGG CGATAGGCCT
    GTCGTGCCGC GAAAGTCGGA CTTTAAGGCT GATCTAGATG CTAAAATACG CAGGCAAAAG
    CAGAAGGTTA AACAGCAGTT GCAGCAGCAG CAGCAGCAAC AGCAGCAAGA GCAGCAAGAA
    ACGCAGCAGC AGCAGCAAGC ACCACAAGAA CAGCAACACT CACCACAGTC GCCCCAAACC
    AGAAACTGTA ATGTCACTAA TCAACAAGCC GCCAATATTA CTGCATCAGC ATCTGCATTT
    GCAACCGCAA CAGCATCCAC AGACCCGTAC CCGAATCCAA ATCATAGAAT GCCAAACCAA
    AATCAGACAG CCACATCCAA TCACACGCAG TGCAAGACGC CCACAATGGC ATTGTCACCG
    TCATCACCTC GCGGCCATTT GCCATTATCA TCGTCATCGC TATCGTCATT ACCATTACCA
    GCCACCACTT CATCACCATC AAATGCCCGG CCATCGATGT TGCCCGCCAG TGACCGACCA
    CCCGCCCATC CATATGTGTG CTCCAATGCC CCAGCCAATC CCCACCACGC CAATAGCATT
    TCCAATGCCA ATGCCCATCT CAAGCCGTGC ATTACGCCCC GGCCGGCTTC TTTGTCGGGA
    GGAGCAGCCG GCGGTGGCTC CACGCGCATC GGGCGTCGAT CGTCCATTAA TCAGTCCAAG
    CCACCGCCGC CAGTCAGACG CAGTTCGTCG GTGACCCCCA GTCCCAATGC CTCGGTCGGG
    CTGCAGCAGC AACCACAGCA CGCGACTCTG TCGCAGCAGA ATCACCAACT AAGCAGCTCC
    AGCGAGCACT TACCGCCGCC ACCGCCATTC ATGCTGGACG CCATGCCCCA GATTCCCAGC
    TCAGCGCTGA AGGTATCGGA GACGGTAAGA GCCCTGGCAG CCATGCGGCA CCAGCCAGCA
    TCACCTGTGT CCCTCAGACG CATGCAGCAG CAACAGCAGC AGCAGCTTCA GCAGCAACAG
    CAGCAACAGG TGCAGCAGCA GCAACCCCTA TTGCAGTCTG CGCACAACTC CCCCCTCAAA
    GAGGACCTGA CCGTGTACTA CGACTCCTAC TTGGATCTGC ACGCCTATGC CCAGGCCTTG
    GCCAGCGGCC AACAGCCCGG CGGTCAGCAG ATGGCCAACC AGCAACGCTT TACCCTCCAG
    CAGCAACATC AGCATCTGCA TCAGCCACAG CAACCGCCTG TCTACCAAGT CGATGCCACG
    TTCCGCACCT CATCACCAGC CGCGGGCGGA GGGGGTGGCG GCGGCATATA CGCCCAGCCC
    AAACTGGTCA ACAGCATGTC CAGCTTCCGC ACCAGCAGCC CCAGTCCCAA CGGACATGCT
    CACCCACTGC CACCGACACA GCCAAAGGCG AATCCGAACC TAATTGCACA GCTCAATGCA
    CGACTCAGCG GCAAACAGCA GCAGCACCAA CAGCAGCAGC ATATCGAGGG GATCTACGGC
    AACCAGCAGG CGCCCGGAGG AGAGTCGATC TACATGCGGA GTGGCCTGTC CATGTCGCAG
    CCGCAACAGC AGCAACACTT TGACGGTAAA TCTGAACAAA TCCAGCTGCA GCACCAGCAA
    CAGCATAGAA TTTACGCTAG TTTCGGCACC TCATCATCAG CAATGTCATC CGCTCATGCG
    GCCAACAGCA GCACTAAACC GTCCATTCTA ACACCGACCA CCTCTTTCAA TGCATTGCCT
    CACTTCCCCC TGTCTTCATC CACATCATCG TTGCTCTCCA AAGTCAGCTC ATTCTCGAAC
    TCTTCATCCG CATCCCCACC GACAACGGCA GCGACCTCTG GCTCGGCCAG TTCGCATTAT
    CAGCCACCTC AGCCGCCGAA TGCAGCAGTT GCTAACAGCA AAGACATGGC CATCTACTCA
    AGTTCGTTTA CCAAAAATCC AGCAGCTGCG CAATCGCCGA ACATGAGACA GGCTCATTCC
    CATCAGCACC ACCAGCCGCC GCAGCAGCAG CACTACACCT GTCCGCCTCC TCTGGAGGAT
    CCCCCACCGC CGCCCATTTA CGCCGCCGGT GCATCGGCCA CGATGCCCAA GAAGATGGCC
    CGTCCGCCCA CTGGCCAGAA CGCGACTCAC TCGAGCGCCT ATGCAGCAGC CTCGTCTACG
    GCCACGCTGC CCAAGAATAT GATGCAGCAG CAGCAGCGGT TGCAGCACCA GCAGCAGTAT
    CAACAGCCGG CAGGCATGGG CATTGGCAAC GGCAATGGGC ACCTAGGTCA GCGTCCGCAG
    TTGCCGCTGC CCCAGCAGAA GCTTAGAGCT GCACAGCAGC AGCACTTGGC GGAGCAGCAG
    CATCAGCAAC AGCAAGAACA GCAGCAGCAC CACCAGCAGC ACCAACAACA CCAGCAACGC
    CAGCCGCCCA TTCCTTCACG CCACTCAAGT GTGCAGCAAA AGATATTCGT ATCAACGAAT
    CCATTCATAC AGACAACGGC CGTCAAGTTT CACTCGCCCT CGGCCTCGCC CACGTGCGGC
    TCGCCCGTTA CTGGGTCCTT GGCTAGCATT TATGCCACAA CCTCGCGTGG CGGCCACCAT
    CACCAGCAGC AGCAGGCTCA GCAGCAGCAG CACTACTATC GCGATGCTGC CGGGGGCAAC
    AGCAACGGCG GCGCTGCCTA CTATAACCAC AATGCCCATG CCCATTCCCA GGCACATCAT
    CCAAACTATG CCACAAGCAC AAATATCGAA AAGACTGGCA GTATTCGTGC CAAGACCAAG
    GCCGAATTCC TCGAGAATCT CAACGCGAAG CTGGCGAAGC AGGGAATGTC TGGACGAGCA
    TTTGCCGTGC GAAATCTCAT CAACAGCAAG GCCCTGATGT ATCAAAATCC GCAAAAACTA
    TCGCGACCCA GTGCGCAATA CCGTAGACCA CCCACCTATC CCAACACCAG CACAACCACC
    AATGCCACTT GCGAAGATCA GTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12121
    Clone ID Related Accession (Same CDS sequence) NM_001273818.1
    Accession Version NM_001273818.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product missing-in-metastasis, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG GAGGAGCAGC CGGCGGTGGC
    TCCACGCGCA TCGGGCGTCG ATCGTCCATT AATCAGTCCA AGCCACCGCC GCCAGTCAGA
    CGCAGTTCGT CGGTGACCCC CAGTCCCAAT GCCTCGGTCG GGCTGCAGCA GCAACCACAG
    CACGCGACTC TGTCGCAGCA GAATCACCAA CTAAGCAGCT CCAGCGAGCA CTTACCGCCG
    CCACCGCCAT TCATGCTGGA CGCCATGCCC CAGATTCCCA GCTCAGCGCT GAAGGTATCG
    GAGACGGTAA GAGCCCTGGC AGCCATGCGG CACCAGCCAG CATCACCTGT GTCCCTCAGA
    CGCATGCAGC AGCAACAGCA GCAGCAGCTT CAGCAGCAAC AGCAGCAACA GGTGCAGCAG
    CAGCAACCCC TATTGCAGTC TGCGCACAAC TCCCCCCTCA AAGAGGACCT GACCGTGTAC
    TACGACTCCT ACTTGGATCT GCACGCCTAT GCCCAGGCCT TGGCCAGCGG CCAACAGCCC
    GGCGGTCAGC AGATGGCCAA CCAGCAACGC TTTACCCTCC AGCAGCAACA TCAGCATCTG
    CATCAGCCAC AGCAACCGCC TGTCTACCAA GTCGATGCCA CGTTCCGCAC CTCATCACCA
    GCCGCGGGCG GAGGGGGTGG CGGCGGCATA TACGCCCAGC CCAAACTGGT CAACAGCATG
    TCCAGCTTCC GCACCAGCAG CCCCAGTCCC AACGGACATG CTCACCCACT GCCACCGACA
    CAGCCAAAGG CGAATCCGAA CCTAATTGCA CAGCTCAATG CACGACTCAG CGGCAAACAG
    CAGCAGCACC AACAGCAGCA GCATATCGAG GGGATCTACG GCAACCAGCA GGCGCCCGGA
    GGAGAGTCGA TCTACATGCG GAGTGGCCTG TCCATGTCGC AGCCGCAACA GCAGCAACAC
    TTTGACGCCG GACCCTCGCA TCTGTCACGA ATCGCTGATG GATCAAATCA AGCGCGGAGC
    CACCTTAAAG CGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260747.1
    CDS890..3304
    Translation

    Target ORF information:

    RefSeq Version NM_001273818.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster missing-in-metastasis (mim), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273818.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG GAGGAGCAGC CGGCGGTGGC
    TCCACGCGCA TCGGGCGTCG ATCGTCCATT AATCAGTCCA AGCCACCGCC GCCAGTCAGA
    CGCAGTTCGT CGGTGACCCC CAGTCCCAAT GCCTCGGTCG GGCTGCAGCA GCAACCACAG
    CACGCGACTC TGTCGCAGCA GAATCACCAA CTAAGCAGCT CCAGCGAGCA CTTACCGCCG
    CCACCGCCAT TCATGCTGGA CGCCATGCCC CAGATTCCCA GCTCAGCGCT GAAGGTATCG
    GAGACGGTAA GAGCCCTGGC AGCCATGCGG CACCAGCCAG CATCACCTGT GTCCCTCAGA
    CGCATGCAGC AGCAACAGCA GCAGCAGCTT CAGCAGCAAC AGCAGCAACA GGTGCAGCAG
    CAGCAACCCC TATTGCAGTC TGCGCACAAC TCCCCCCTCA AAGAGGACCT GACCGTGTAC
    TACGACTCCT ACTTGGATCT GCACGCCTAT GCCCAGGCCT TGGCCAGCGG CCAACAGCCC
    GGCGGTCAGC AGATGGCCAA CCAGCAACGC TTTACCCTCC AGCAGCAACA TCAGCATCTG
    CATCAGCCAC AGCAACCGCC TGTCTACCAA GTCGATGCCA CGTTCCGCAC CTCATCACCA
    GCCGCGGGCG GAGGGGGTGG CGGCGGCATA TACGCCCAGC CCAAACTGGT CAACAGCATG
    TCCAGCTTCC GCACCAGCAG CCCCAGTCCC AACGGACATG CTCACCCACT GCCACCGACA
    CAGCCAAAGG CGAATCCGAA CCTAATTGCA CAGCTCAATG CACGACTCAG CGGCAAACAG
    CAGCAGCACC AACAGCAGCA GCATATCGAG GGGATCTACG GCAACCAGCA GGCGCCCGGA
    GGAGAGTCGA TCTACATGCG GAGTGGCCTG TCCATGTCGC AGCCGCAACA GCAGCAACAC
    TTTGACGCCG GACCCTCGCA TCTGTCACGA ATCGCTGATG GATCAAATCA AGCGCGGAGC
    CACCTTAAAG CGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12122
    Clone ID Related Accession (Same CDS sequence) NM_001273820.1
    Accession Version NM_001273820.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product missing-in-metastasis, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG ACAAGTCTTT CGAAGATTCA
    ATACAAGCAC TAAATAATTT AATTGGCGAA CTAGACTCGT TCCAACGCGA GATCGATGAG
    GGCAAGGTCA AACCGCCGAG CAACATCATA AGCGGCAGCA CCACCAGTAG CAACAACAAC
    AATACGACGA CCAGCAGCAT CAGCAGCAGC GACAACAACA ACCTGCCCGC CACCAGCAAC
    ATCGAGCCAT GCGCCATCAG CAATCAGACG AACTCGAGCG GCTGCGGCAC GGACATATCC
    GACACCACGT CCGACGAACT GGCCGGCGAC GATATGGACG TCAGGCGGCG GGATCGGGAT
    CGGGACCGGG ATCTGCTGGG CGCCAGCGAT TCGGAGCTGA GTCGCTGCTA TGTGAGCGAG
    ACGAGTTCGC TGACCGGTGG CCTAACGGCC GGCGGCTACG AGAATCCCAC TTTCGCGCAC
    TTTGCGGCCA ATGCCAATCG GGAGGACGCT GTTTCGCTGG CCTCCGACAG CGTTTGTCTC
    GGCCAGCCAC GCCACGCCTA TGTGGATACC TGCAGCGACA GCGGCAGTGC CGTGGTGGTG
    ATCTACGACC ACCAGATTCC CAACACACCC GACATTGAGT TCGTGAAGCA GAACTCTGAG
    ATTGTAGTGC TGCGGACTAA GGATCCGCAG CCCCACGCGC TGCAGCTGCA CGAGATGCGC
    GAGCTGCAGC AGTTGCCCGC CAATTTGGCC GGTTCGCCGG ACTCCTCGCC GGACTCTGCC
    GGTGGCCAGG CACCGCCAAC AGCAACTGTG GCGCCCGCCA AGCAGCGACT CTCCTCGTTT
    CGCGCCACCA GTGAGCAGCA GTTGCAGCTC CTCGGACGCG GTAGCCCGCA AAGAGGAGGA
    GCAGCCGGCG GTGGCTCCAC GCGCATCGGG CGTCGATCGT CCATTAATCA GTCCAAGCCA
    CCGCCGCCAG TCAGACGCAG TTCGTCGGTG ACCCCCAGTC CCAATGCCTC GGTCGGGCTG
    CAGCAGCAAC CACAGCACGC GACTCTGTCG CAGCAGAATC ACCAACTAAG CAGCTCCAGC
    GAGCACTTAC CGCCGCCACC GCCATTCATG CTGGACGCCA TGCCCCAGAT TCCCAGCTCA
    GCGCTGAAGG TATCGGAGAC GTCTGCGCAC AACTCCCCCC TCAAAGAGGA CCTGACCGTG
    TACTACGACT CCTACTTGGA TCTGCACGCC TATGCCCAGG CCTTGGCCAG CGGCCAACAG
    CCCGGCGGTC AGCAGATGGC CAACCAGCAA CGCTTTACCC TCCAGCAGCA ACATCAGCAT
    CTGCATCAGC CACAGCAACC GCCTGTCTAC CAAGTCGATG CCACGTTCCG CACCTCATCA
    CCAGCCGCGG GCGGAGGGGG TGGCGGCGGC ATATACGCCC AGCCCAAACT GGTCAACAGC
    ATGTCCAGCT TCCGCACCAG CAGCCCCAGT CCCAACGGAC ATGCTCACCC ACTGCCACCG
    ACACAGCCAA AGGCGAATCC GAACCTAATT GCACAGCTCA ATGCACGACT CAGCGGCAAA
    CAGCAGCAGC ACCAACAGCA GCAGCATATC GAGGGGATCT ACGGCAACCA GCAGGCGCCC
    GGAGGAGAGT CGATCTACAT GCGGAGTGGC CTGTCCATGT CGCAGCCGCA ACAGCAGCAA
    CACTTTGACG CAGCTGCGCA ATCGCCGAAC ATGAGACAGG CTCATTCCCA TCAGCACCAC
    CAGCCGCCGC AGCAGCAGCA CTACACCTGT CCGCCTCCTC TGGAGGATCC CCCACCGCCG
    CCCATTTACG CCGCCGGTGC ATCGGCCACG ATGCCCAAGA AGATGGCCCG TCCGCCCACT
    GGCCAGAACG CGACTCACTC GAGCGCCTAT GCAGCAGCCT CGTCTACGGC CACGCTGCCC
    AAGAATATGA TGCAGCAGCA GCAGCGGTTG CAGCACCAGC AGCAGTATCA ACAGCCGGCA
    GGCATGGGCA TTGGCAACGG CAATGGGCAC CTAGGTCAGC GTCCGCAGTT GCCGCTGCCC
    CAGCAGAAGC TTAGAGCTGC ACAGCAGCAG CACTTGGCGG AGCAGCAGCA TCAGCAACAG
    CAAGAACAGC AGCAGCACCA CCAGCAGCAC CAACAACACC AGCAACGCCA GCCGCCCATT
    CCTTCACGCC ACTCAAGTGT GCAGCAAAAG ATATTCGTAT CAACGAATCC ATTCATACAG
    ACAACGGCCG TCAAGTTTCA CTCGCCCTCG GCCTCGCCCA CGTGCGGCTC GCCCGTTACT
    GGGTCCTTGG CTAGCATTTA TGCCACAACC TCGCGTGGCG GCCACCATCA CCAGCAGCAG
    CAGGCTCAGC AGCAGCAGCA CTACTATCGC GATGCTGCCG GGGGCAACAG CAACGGCGGC
    GCTGCCTACT ATAACCACAA TGCCCATGCC CATTCCCAGG CACATCATCC AACCGGACCC
    TCGCATCTGT CACGAATCGC TGATGGATCA AATCAAGCGC GGAGCCACCT TAAAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260749.1
    CDS890..4789
    Translation

    Target ORF information:

    RefSeq Version NM_001273820.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster missing-in-metastasis (mim), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273820.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG ACAAGTCTTT CGAAGATTCA
    ATACAAGCAC TAAATAATTT AATTGGCGAA CTAGACTCGT TCCAACGCGA GATCGATGAG
    GGCAAGGTCA AACCGCCGAG CAACATCATA AGCGGCAGCA CCACCAGTAG CAACAACAAC
    AATACGACGA CCAGCAGCAT CAGCAGCAGC GACAACAACA ACCTGCCCGC CACCAGCAAC
    ATCGAGCCAT GCGCCATCAG CAATCAGACG AACTCGAGCG GCTGCGGCAC GGACATATCC
    GACACCACGT CCGACGAACT GGCCGGCGAC GATATGGACG TCAGGCGGCG GGATCGGGAT
    CGGGACCGGG ATCTGCTGGG CGCCAGCGAT TCGGAGCTGA GTCGCTGCTA TGTGAGCGAG
    ACGAGTTCGC TGACCGGTGG CCTAACGGCC GGCGGCTACG AGAATCCCAC TTTCGCGCAC
    TTTGCGGCCA ATGCCAATCG GGAGGACGCT GTTTCGCTGG CCTCCGACAG CGTTTGTCTC
    GGCCAGCCAC GCCACGCCTA TGTGGATACC TGCAGCGACA GCGGCAGTGC CGTGGTGGTG
    ATCTACGACC ACCAGATTCC CAACACACCC GACATTGAGT TCGTGAAGCA GAACTCTGAG
    ATTGTAGTGC TGCGGACTAA GGATCCGCAG CCCCACGCGC TGCAGCTGCA CGAGATGCGC
    GAGCTGCAGC AGTTGCCCGC CAATTTGGCC GGTTCGCCGG ACTCCTCGCC GGACTCTGCC
    GGTGGCCAGG CACCGCCAAC AGCAACTGTG GCGCCCGCCA AGCAGCGACT CTCCTCGTTT
    CGCGCCACCA GTGAGCAGCA GTTGCAGCTC CTCGGACGCG GTAGCCCGCA AAGAGGAGGA
    GCAGCCGGCG GTGGCTCCAC GCGCATCGGG CGTCGATCGT CCATTAATCA GTCCAAGCCA
    CCGCCGCCAG TCAGACGCAG TTCGTCGGTG ACCCCCAGTC CCAATGCCTC GGTCGGGCTG
    CAGCAGCAAC CACAGCACGC GACTCTGTCG CAGCAGAATC ACCAACTAAG CAGCTCCAGC
    GAGCACTTAC CGCCGCCACC GCCATTCATG CTGGACGCCA TGCCCCAGAT TCCCAGCTCA
    GCGCTGAAGG TATCGGAGAC GTCTGCGCAC AACTCCCCCC TCAAAGAGGA CCTGACCGTG
    TACTACGACT CCTACTTGGA TCTGCACGCC TATGCCCAGG CCTTGGCCAG CGGCCAACAG
    CCCGGCGGTC AGCAGATGGC CAACCAGCAA CGCTTTACCC TCCAGCAGCA ACATCAGCAT
    CTGCATCAGC CACAGCAACC GCCTGTCTAC CAAGTCGATG CCACGTTCCG CACCTCATCA
    CCAGCCGCGG GCGGAGGGGG TGGCGGCGGC ATATACGCCC AGCCCAAACT GGTCAACAGC
    ATGTCCAGCT TCCGCACCAG CAGCCCCAGT CCCAACGGAC ATGCTCACCC ACTGCCACCG
    ACACAGCCAA AGGCGAATCC GAACCTAATT GCACAGCTCA ATGCACGACT CAGCGGCAAA
    CAGCAGCAGC ACCAACAGCA GCAGCATATC GAGGGGATCT ACGGCAACCA GCAGGCGCCC
    GGAGGAGAGT CGATCTACAT GCGGAGTGGC CTGTCCATGT CGCAGCCGCA ACAGCAGCAA
    CACTTTGACG CAGCTGCGCA ATCGCCGAAC ATGAGACAGG CTCATTCCCA TCAGCACCAC
    CAGCCGCCGC AGCAGCAGCA CTACACCTGT CCGCCTCCTC TGGAGGATCC CCCACCGCCG
    CCCATTTACG CCGCCGGTGC ATCGGCCACG ATGCCCAAGA AGATGGCCCG TCCGCCCACT
    GGCCAGAACG CGACTCACTC GAGCGCCTAT GCAGCAGCCT CGTCTACGGC CACGCTGCCC
    AAGAATATGA TGCAGCAGCA GCAGCGGTTG CAGCACCAGC AGCAGTATCA ACAGCCGGCA
    GGCATGGGCA TTGGCAACGG CAATGGGCAC CTAGGTCAGC GTCCGCAGTT GCCGCTGCCC
    CAGCAGAAGC TTAGAGCTGC ACAGCAGCAG CACTTGGCGG AGCAGCAGCA TCAGCAACAG
    CAAGAACAGC AGCAGCACCA CCAGCAGCAC CAACAACACC AGCAACGCCA GCCGCCCATT
    CCTTCACGCC ACTCAAGTGT GCAGCAAAAG ATATTCGTAT CAACGAATCC ATTCATACAG
    ACAACGGCCG TCAAGTTTCA CTCGCCCTCG GCCTCGCCCA CGTGCGGCTC GCCCGTTACT
    GGGTCCTTGG CTAGCATTTA TGCCACAACC TCGCGTGGCG GCCACCATCA CCAGCAGCAG
    CAGGCTCAGC AGCAGCAGCA CTACTATCGC GATGCTGCCG GGGGCAACAG CAACGGCGGC
    GCTGCCTACT ATAACCACAA TGCCCATGCC CATTCCCAGG CACATCATCC AACCGGACCC
    TCGCATCTGT CACGAATCGC TGATGGATCA AATCAAGCGC GGAGCCACCT TAAAGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12123
    Clone ID Related Accession (Same CDS sequence) NM_001201939.3
    Accession Version NM_001201939.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product missing-in-metastasis, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001201939.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG AGCTGTCCGC AGCGCATCAT
    GCACAGCAGC AGCAGCAGCA GCAAAATCAG CAGCCCCAGA CGCCGCCCAC CTATGTGAAC
    ATGTCGGAGC TGGCCACCAT GGCCGCTTTG AAGCAGACCA ATCAGCAGCA GAAGCCCTCT
    ACGCCGCCTC TGCAGCAGCA GAGCTCCATT GACTCGACCA GCTCCCAGCA TTCCAACGAC
    TCCACCGGCT CTCATCAGCT CCTCCAGCAG CAGCAGCAGC ATCATCAATC GCAGCAGAAT
    CACCACTCAG CCACTGCCAC ACGCTCCCAT TCCATATCCT CGACGGCCTC GTCACTTCAC
    TCGCATCCGT CGATCGACTC CACCGTCGCT TGCGGCTCGC TGGTGGGCCA GCACAACCAC
    AGCACCAGCA CCAACACGAA CACCACCTCG CCGTCCAGTG GCAGCTCCAC GCCACAGAAC
    CATTACTCGC CCCTGCTAAC CAACTCCCCC ACGTCCACTG CCGCAGGTAC TCCCAGTGGC
    AGCAGCTTAG GTCCTGGGTC CGGTTTGGGA TTTGTCTACC AGGTCAGCTC CCCGACGCCT
    CCCTCCAGCG AGGTGCTGAA AATCACCGAG CAAGCCGCTG CAGGACAGGA TCAGGGTCCA
    GCCAACAGCG TAGCGGACGA GACGGATGAG CGATCAAGGG CCTCAGTCCT GCAGAAGGCT
    TCAATGTTCG AAAAGGCGGC AGCAGCGGCA GCGGTATCGC CCCCAGCTCC CATGCAGATT
    GCATCCGGTT CCCCAGCTTC GGGAGGCGGA ACTCGACGAT CCGAGGCGGA GCAGCAGGAA
    ATGGGAGGAG CAGCCGGCGG TGGCTCCACG CGCATCGGGC GTCGATCGTC CATTAATCAG
    TCCAAGCCAC CGCCGCCAGT CAGACGCAGT TCGTCGGTGA CCCCCAGTCC CAATGCCTCG
    GTCGGGACGT TCCGCACCTC ATCACCAGCC GCGGGCGGAG GGGGTGGCGG CGGCATATAC
    GCCCAGCCCA AACTGGTCAA CAGCATGTCC AGCTTCCGCA CCAGCAGCCC CAGTCCCAAC
    GGACATGCTC ACCCACTGCC ACCGACACAG CCAAAGGCGA ATCCGAACCT AATTGCACAG
    CTCAATGCAC GACTCAGCGG CAAACAGCAG CAGCACCAAC AGCAGCAGCA TATCGAGGGG
    ATCTACGGCA ACCAGCAGGC GCCCGGAGGA GAGTCGATCT ACATGCGGAG TGGCCTGTCC
    ATGTCGCAGC CGCAACAGCA GCAACACTTT GACGACTATG CCACAAGCAC AAATATCGAA
    AAGACTGGCA GTATTCGTGC CAAGACCAAG GCCGAATTCC TCGAGAATCT CAACGCGAAG
    CTGGCGAAGC AGGGAATGTC TGGACGAGCA TTTGCCGTGC GAAATCTCAT CAACAGCAAG
    GCCCTGCCGG ACCCTCGCAT CTGTCACGAA TCGCTGATGG ATCAAATCAA GCGCGGAGCC
    ACCTTAAAGC GTAACCAGAA GATCAACGAT CGCAGCGCTC CCAAGATACA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188868.1
    CDS890..3823
    Translation

    Target ORF information:

    RefSeq Version NM_001201939.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster missing-in-metastasis (mim), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001201939.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG AGCTGTCCGC AGCGCATCAT
    GCACAGCAGC AGCAGCAGCA GCAAAATCAG CAGCCCCAGA CGCCGCCCAC CTATGTGAAC
    ATGTCGGAGC TGGCCACCAT GGCCGCTTTG AAGCAGACCA ATCAGCAGCA GAAGCCCTCT
    ACGCCGCCTC TGCAGCAGCA GAGCTCCATT GACTCGACCA GCTCCCAGCA TTCCAACGAC
    TCCACCGGCT CTCATCAGCT CCTCCAGCAG CAGCAGCAGC ATCATCAATC GCAGCAGAAT
    CACCACTCAG CCACTGCCAC ACGCTCCCAT TCCATATCCT CGACGGCCTC GTCACTTCAC
    TCGCATCCGT CGATCGACTC CACCGTCGCT TGCGGCTCGC TGGTGGGCCA GCACAACCAC
    AGCACCAGCA CCAACACGAA CACCACCTCG CCGTCCAGTG GCAGCTCCAC GCCACAGAAC
    CATTACTCGC CCCTGCTAAC CAACTCCCCC ACGTCCACTG CCGCAGGTAC TCCCAGTGGC
    AGCAGCTTAG GTCCTGGGTC CGGTTTGGGA TTTGTCTACC AGGTCAGCTC CCCGACGCCT
    CCCTCCAGCG AGGTGCTGAA AATCACCGAG CAAGCCGCTG CAGGACAGGA TCAGGGTCCA
    GCCAACAGCG TAGCGGACGA GACGGATGAG CGATCAAGGG CCTCAGTCCT GCAGAAGGCT
    TCAATGTTCG AAAAGGCGGC AGCAGCGGCA GCGGTATCGC CCCCAGCTCC CATGCAGATT
    GCATCCGGTT CCCCAGCTTC GGGAGGCGGA ACTCGACGAT CCGAGGCGGA GCAGCAGGAA
    ATGGGAGGAG CAGCCGGCGG TGGCTCCACG CGCATCGGGC GTCGATCGTC CATTAATCAG
    TCCAAGCCAC CGCCGCCAGT CAGACGCAGT TCGTCGGTGA CCCCCAGTCC CAATGCCTCG
    GTCGGGACGT TCCGCACCTC ATCACCAGCC GCGGGCGGAG GGGGTGGCGG CGGCATATAC
    GCCCAGCCCA AACTGGTCAA CAGCATGTCC AGCTTCCGCA CCAGCAGCCC CAGTCCCAAC
    GGACATGCTC ACCCACTGCC ACCGACACAG CCAAAGGCGA ATCCGAACCT AATTGCACAG
    CTCAATGCAC GACTCAGCGG CAAACAGCAG CAGCACCAAC AGCAGCAGCA TATCGAGGGG
    ATCTACGGCA ACCAGCAGGC GCCCGGAGGA GAGTCGATCT ACATGCGGAG TGGCCTGTCC
    ATGTCGCAGC CGCAACAGCA GCAACACTTT GACGACTATG CCACAAGCAC AAATATCGAA
    AAGACTGGCA GTATTCGTGC CAAGACCAAG GCCGAATTCC TCGAGAATCT CAACGCGAAG
    CTGGCGAAGC AGGGAATGTC TGGACGAGCA TTTGCCGTGC GAAATCTCAT CAACAGCAAG
    GCCCTGCCGG ACCCTCGCAT CTGTCACGAA TCGCTGATGG ATCAAATCAA GCGCGGAGCC
    ACCTTAAAGC GTAACCAGAA GATCAACGAT CGCAGCGCTC CCAAGATACA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12124
    Clone ID Related Accession (Same CDS sequence) NM_001259228.2
    Accession Version NM_001259228.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product missing-in-metastasis, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259228.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG AGCTGTCCGC AGCGCATCAT
    GCACAGCAGC AGCAGCAGCA GCAAAATCAG CAGCCCCAGA CGCCGCCCAC CTATGTGAAC
    ATGTCGGAGC TGGCCACCAT GGCCGCTTTG AAGCAGACCA ATCAGCAGCA GAAGCCCTCT
    ACGCCGCCTC TGCAGCAGCA GAGCTCCATT GACTCGACCA GCTCCCAGCA TTCCAACGAC
    TCCACCGGCT CTCATCAGCT CCTCCAGCAG CAGCAGCAGC ATCATCAATC GCAGCAGAAT
    CACCACTCAG CCACTGCCAC ACGCTCCCAT TCCATATCCT CGACGGCCTC GTCACTTCAC
    TCGCATCCGT CGATCGACTC CACCGTCGCT TGCGGCTCGC TGGTGGGCCA GCACAACCAC
    AGCACCAGCA CCAACACGAA CACCACCTCG CCGTCCAGTG GCAGCTCCAC GCCACAGAAC
    CATTACTCGC CCCTGCTAAC CAACTCCCCC ACGTCCACTG CCGCAGGTAC TCCCAGTGGC
    AGCAGCTTAG GTCCTGGGTC CGGTTTGGGA TTTGTCTACC AGGTCAGCTC CCCGACGCCT
    CCCTCCAGCG AGGTGCTGAA AATCACCGAG CAAGCCGCTG CAGGACAGGA TCAGGGTCCA
    GCCAACAGCG TAGCGGACGA GACGGATGAG CGATCAAGGG CCTCAGTCCT GCAGAAGGCT
    TCAATGTTCG AAAAGGCGGC AGCAGCGGCA GCGGTATCGC CCCCAGCTCC CATGCAGATT
    GCATCCGGTT CCCCAGCTTC GGGAGGCGGA ACTCGACGAT CCGAGGCGGA GCAGCAGGAA
    ATGGGAGGAG CAGCCGGCGG TGGCTCCACG CGCATCGGGC GTCGATCGTC CATTAATCAG
    TCCAAGCCAC CGCCGCCAGT CAGACGCAGT TCGTCGGTGA CCCCCAGTCC CAATGCCTCG
    GTCGGGCTGC AGCAGCAACC ACAGCACGCG ACTCTGTCGC AGCAGAATCA CCAACTAAGC
    AGCTCCAGCG AGCACTTACC GCCGCCACCG CCATTCATGC TGGACGCCAT GCCCCAGATT
    CCCAGCTCAG CGCTGAAGGT ATCGGAGACG GTAAGAGCCC TGGCAGCCAT GCGGCACCAG
    CCAGCATCAC CTGTGTCCCT CAGACGCATG CAGCAGCAAC AGCAGCAGCA GCTTCAGCAG
    CAACAGCAGC AACAGGTGCA GCAGCAGCAA CCCCTATTGC AGTCTGCGCA CAACTCCCCC
    CTCAAAGAGG ACCTGACCGT GTACTACGAC TCCTACTTGG ATCTGCACGC CTATGCCCAG
    GCCTTGGCCA GCGGCCAACA GCCCGGCGGT CAGCAGATGG CCAACCAGCA ACGCTTTACC
    CTCCAGCAGC AACATCAGCA TCTGCATCAG CCACAGCAAC CGCCTGTCTA CCAAGTCGAT
    GCCACGTTCC GCACCTCATC ACCAGCCGCG GGCGGAGGGG GTGGCGGCGG CATATACGCC
    CAGCCCAAAC TGGTCAACAG CATGTCCAGC TTCCGCACCA GCAGCCCCAG TCCCAACGGA
    CATGCTCACC CACTGCCACC GACACAGCCA AAGGCGAATC CGAACCTAAT TGCACAGCTC
    AATGCACGAC TCAGCGGCAA ACAGCAGCAG CACCAACAGC AGCAGCATAT CGAGGGGATC
    TACGGCAACC AGCAGGCGCC CGGAGGAGAG TCGATCTACA TGCGGAGTGG CCTGTCCATG
    TCGCAGCCGC AACAGCAGCA ACACTTTGAC GACTATGCCA CAAGCACAAA TATCGAAAAG
    ACTGGCAGTA TTCGTGCCAA GACCAAGGCC GAATTCCTCG AGAATCTCAA CGCGAAGCTG
    GCGAAGCAGG GAATGTCTGG ACGAGCATTT GCCGTGCGAA ATCTCATCAA CAGCAAGGCC
    CTGCCGGACC CTCGCATCTG TCACGAATCG CTGATGGATC AAATCAAGCG CGGAGCCACC
    TTAAAGCGTA ACCAGAAGAT CAACGATCGC AGCGCTCCCA AGATACATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246157.1
    CDS890..4300
    Translation

    Target ORF information:

    RefSeq Version NM_001259228.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster missing-in-metastasis (mim), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259228.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG AGCTGTCCGC AGCGCATCAT
    GCACAGCAGC AGCAGCAGCA GCAAAATCAG CAGCCCCAGA CGCCGCCCAC CTATGTGAAC
    ATGTCGGAGC TGGCCACCAT GGCCGCTTTG AAGCAGACCA ATCAGCAGCA GAAGCCCTCT
    ACGCCGCCTC TGCAGCAGCA GAGCTCCATT GACTCGACCA GCTCCCAGCA TTCCAACGAC
    TCCACCGGCT CTCATCAGCT CCTCCAGCAG CAGCAGCAGC ATCATCAATC GCAGCAGAAT
    CACCACTCAG CCACTGCCAC ACGCTCCCAT TCCATATCCT CGACGGCCTC GTCACTTCAC
    TCGCATCCGT CGATCGACTC CACCGTCGCT TGCGGCTCGC TGGTGGGCCA GCACAACCAC
    AGCACCAGCA CCAACACGAA CACCACCTCG CCGTCCAGTG GCAGCTCCAC GCCACAGAAC
    CATTACTCGC CCCTGCTAAC CAACTCCCCC ACGTCCACTG CCGCAGGTAC TCCCAGTGGC
    AGCAGCTTAG GTCCTGGGTC CGGTTTGGGA TTTGTCTACC AGGTCAGCTC CCCGACGCCT
    CCCTCCAGCG AGGTGCTGAA AATCACCGAG CAAGCCGCTG CAGGACAGGA TCAGGGTCCA
    GCCAACAGCG TAGCGGACGA GACGGATGAG CGATCAAGGG CCTCAGTCCT GCAGAAGGCT
    TCAATGTTCG AAAAGGCGGC AGCAGCGGCA GCGGTATCGC CCCCAGCTCC CATGCAGATT
    GCATCCGGTT CCCCAGCTTC GGGAGGCGGA ACTCGACGAT CCGAGGCGGA GCAGCAGGAA
    ATGGGAGGAG CAGCCGGCGG TGGCTCCACG CGCATCGGGC GTCGATCGTC CATTAATCAG
    TCCAAGCCAC CGCCGCCAGT CAGACGCAGT TCGTCGGTGA CCCCCAGTCC CAATGCCTCG
    GTCGGGCTGC AGCAGCAACC ACAGCACGCG ACTCTGTCGC AGCAGAATCA CCAACTAAGC
    AGCTCCAGCG AGCACTTACC GCCGCCACCG CCATTCATGC TGGACGCCAT GCCCCAGATT
    CCCAGCTCAG CGCTGAAGGT ATCGGAGACG GTAAGAGCCC TGGCAGCCAT GCGGCACCAG
    CCAGCATCAC CTGTGTCCCT CAGACGCATG CAGCAGCAAC AGCAGCAGCA GCTTCAGCAG
    CAACAGCAGC AACAGGTGCA GCAGCAGCAA CCCCTATTGC AGTCTGCGCA CAACTCCCCC
    CTCAAAGAGG ACCTGACCGT GTACTACGAC TCCTACTTGG ATCTGCACGC CTATGCCCAG
    GCCTTGGCCA GCGGCCAACA GCCCGGCGGT CAGCAGATGG CCAACCAGCA ACGCTTTACC
    CTCCAGCAGC AACATCAGCA TCTGCATCAG CCACAGCAAC CGCCTGTCTA CCAAGTCGAT
    GCCACGTTCC GCACCTCATC ACCAGCCGCG GGCGGAGGGG GTGGCGGCGG CATATACGCC
    CAGCCCAAAC TGGTCAACAG CATGTCCAGC TTCCGCACCA GCAGCCCCAG TCCCAACGGA
    CATGCTCACC CACTGCCACC GACACAGCCA AAGGCGAATC CGAACCTAAT TGCACAGCTC
    AATGCACGAC TCAGCGGCAA ACAGCAGCAG CACCAACAGC AGCAGCATAT CGAGGGGATC
    TACGGCAACC AGCAGGCGCC CGGAGGAGAG TCGATCTACA TGCGGAGTGG CCTGTCCATG
    TCGCAGCCGC AACAGCAGCA ACACTTTGAC GACTATGCCA CAAGCACAAA TATCGAAAAG
    ACTGGCAGTA TTCGTGCCAA GACCAAGGCC GAATTCCTCG AGAATCTCAA CGCGAAGCTG
    GCGAAGCAGG GAATGTCTGG ACGAGCATTT GCCGTGCGAA ATCTCATCAA CAGCAAGGCC
    CTGCCGGACC CTCGCATCTG TCACGAATCG CTGATGGATC AAATCAAGCG CGGAGCCACC
    TTAAAGCGTA ACCAGAAGAT CAACGATCGC AGCGCTCCCA AGATACATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12125
    Clone ID Related Accession (Same CDS sequence) NM_001273821.1
    Accession Version NM_001273821.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product missing-in-metastasis, isoform N
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG AGCTGTCCGC AGCGCATCAT
    GCACAGCAGC AGCAGCAGCA GCAAAATCAG CAGCCCCAGA CGCCGCCCAC CTATGTGAAC
    ATGTCGGAGC TGGCCACCAT GGCCGCTTTG AAGCAGACCA ATCAGCAGCA GAAGCCCTCT
    ACGCCGCCTC TGCAGCAGCA GAGCTCCATT GACTCGACCA GCTCCCAGCA TTCCAACGAC
    TCCACCGGCT CTCATCAGCT CCTCCAGCAG CAGCAGCAGC ATCATCAATC GCAGCAGAAT
    CACCACTCAG CCACTGCCAC ACGCTCCCAT TCCATATCCT CGACGGCCTC GTCACTTCAC
    TCGCATCCGT CGATCGACTC CACCGTCGCT TGCGGCTCGC TGGTGGGCCA GCACAACCAC
    AGCACCAGCA CCAACACGAA CACCACCTCG CCGTCCAGTG GCAGCTCCAC GCCACAGAAC
    CATTACTCGC CCCTGCTAAC CAACTCCCCC ACGTCCACTG CCGCAGGTAC TCCCAGTGGC
    AGCAGCTTAG GTCCTGGGTC CGGTTTGGGA TTTGTCTACC AGGTCAGCTC CCCGACGCCT
    CCCTCCAGCG AGGTGCTGAA AATCACCGAG CAAGCCGCTG CAGGACAGGA TCAGGGTCCA
    GCCAACAGCG TAGCGGACGA GACGGATGAG CGATCAAGGG CCTCAGTCCT GCAGAAGGCT
    TCAATGTTCG AAAAGGCGGC AGCAGCGGCA GCGGTATCGC CCCCAGCTCC CATGCAGATT
    GCATCCGGTT CCCCAGCTTC GGGAGGCGGA ACTCGACGAT CCGAGGCGGA GCAGCAGGAA
    ATGGACAAGT CTTTCGAAGA TTCAATACAA GCACTAAATA ATTTAATTGG CGAACTAGAC
    TCGTTCCAAC GCGAGATCGA TGAGGGCAAG GTCAAACCGC CGAGCAACAT CATAAGCGGC
    AGCACCACCA GTAGCAACAA CAACAATACG ACGACCAGCA GCATCAGCAG CAGCGACAAC
    AACAACCTGC CCGCCACCAG CAACATCGAG CCATGCGCCA TCAGCAATCA GACGAACTCG
    AGCGGCTGCG GCACGGACAT ATCCGACACC ACGTCCGACG AACTGGCCGG CGACGATATG
    GACGTCAGGC GGCGGGATCG GGATCGGGAC CGGGATCTGC TGGGCGCCAG CGATTCGGAG
    CTGAGTCGCT GCTATGTGAG CGAGACGAGT TCGCTGACCG GTGGCCTAAC GGCCGGCGGC
    TACGAGAATC CCACTTTCGC GCACTTTGCG GCCAATGCCA ATCGGGAGGA CGCTGTTTCG
    CTGGCCTCCG ACAGCGTTTG TCTCGGCCAG CCACGCCACG CCTATGTGGA TACCTGCAGC
    GACAGCGGCA GTGCCGTGGT GGTGATCTAC GACCACCAGA TTCCCAACAC ACCCGACATT
    GAGTTCGTGA AGCAGAACTC TGAGATTGTA GTGCTGCGGA CTAAGGATCC GCAGCCCCAC
    GCGCTGCAGC TGCACGAGAT GCGCGAGCTG CAGCAGTTGC CCGCCAATTT GGCCGGTTCG
    CCGGACTCCT CGCCGGACTC TGCCGGTGGC CAGGCACCGC CAACAGCAAC TGTGGCGCCC
    GCCAAGCAGC GACTCTCCTC GTTTCGCGCC ACCAGTGAGC AGCAGTTGCA GCTCCTCGGA
    CGCGGTAGCC CGCAAAGAGG TAAAACACCC AGTGAGCAGG CGGTACAAAG CAGGCCACAG
    GACCAGCATT TTCCACAGAC ACAACAGCAG GATATTGATG GCAGTAGTCC ACCAGTAGAA
    CTTGCAAGGC GCCAGCTGCC CCCCAAGCCC ACCAGTTTGA GTATTTTTAA CGGCCCCGTG
    CCCACTGCGG GCGATAGGCC TGTCGTGCCG CGAAAGTCGG ACTTTAAGGC TGATCTAGAT
    GCTAAAATAC GCAGGCAAAA GCAGAAGGTT AAACAGCAGT TGCAGCAGCA GCAGCAGCAA
    CAGCAGCAAG AGCAGCAAGA AACGCAGCAG CAGCAGCAAG CACCACAAGA ACAGCAACAC
    TCACCACAGT CGCCCCAAAC CAGAAACTGT AATGTCACTA ATCAACAAGC CGCCAATATT
    ACTGCATCAG CATCTGCATT TGCAACCGCA ACAGCATCCA CAGACCCGTA CCCGAATCCA
    AATCATAGAA TGCCAAACCA AAATCAGACA GCCACATCCA ATCACACGCA GTGCAAGACG
    CCCACAATGG CATTGTCACC GTCATCACCT CGCGGCCATT TGCCATTATC ATCGTCATCG
    CTATCGTCAT TACCATTACC AGCCACCACT TCATCACCAT CAAATGCCCG GCCATCGATG
    TTGCCCGCCA GTGACCGACC ACCCGCCCAT CCATATGTGT GCTCCAATGC CCCAGCCAAT
    CCCCACCACG CCAATAGCAT TTCCAATGCC AATGCCCATC TCAAGCCGTG CATTACGCCC
    CGGCCGGCTT CTTTGTCGGG AGGAGCAGCC GGCGGTGGCT CCACGCGCAT CGGGCGTCGA
    TCGTCCATTA ATCAGTCCAA GCCACCGCCG CCAGTCAGAC GCAGTTCGTC GGTGACCCCC
    AGTCCCAATG CCTCGGTCGG GCCGGACCCT CGCATCTGTC ACGAATCGCT GATGGATCAA
    ATCAAGCGCG GAGCCACCTT AAAGCGTAAC CAGAAGATCA ACGATCGCAG CGCTCCCAAG
    ATACATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260750.1
    CDS890..4978
    Translation

    Target ORF information:

    RefSeq Version NM_001273821.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster missing-in-metastasis (mim), transcript variant N, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273821.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG AGCTGTCCGC AGCGCATCAT
    GCACAGCAGC AGCAGCAGCA GCAAAATCAG CAGCCCCAGA CGCCGCCCAC CTATGTGAAC
    ATGTCGGAGC TGGCCACCAT GGCCGCTTTG AAGCAGACCA ATCAGCAGCA GAAGCCCTCT
    ACGCCGCCTC TGCAGCAGCA GAGCTCCATT GACTCGACCA GCTCCCAGCA TTCCAACGAC
    TCCACCGGCT CTCATCAGCT CCTCCAGCAG CAGCAGCAGC ATCATCAATC GCAGCAGAAT
    CACCACTCAG CCACTGCCAC ACGCTCCCAT TCCATATCCT CGACGGCCTC GTCACTTCAC
    TCGCATCCGT CGATCGACTC CACCGTCGCT TGCGGCTCGC TGGTGGGCCA GCACAACCAC
    AGCACCAGCA CCAACACGAA CACCACCTCG CCGTCCAGTG GCAGCTCCAC GCCACAGAAC
    CATTACTCGC CCCTGCTAAC CAACTCCCCC ACGTCCACTG CCGCAGGTAC TCCCAGTGGC
    AGCAGCTTAG GTCCTGGGTC CGGTTTGGGA TTTGTCTACC AGGTCAGCTC CCCGACGCCT
    CCCTCCAGCG AGGTGCTGAA AATCACCGAG CAAGCCGCTG CAGGACAGGA TCAGGGTCCA
    GCCAACAGCG TAGCGGACGA GACGGATGAG CGATCAAGGG CCTCAGTCCT GCAGAAGGCT
    TCAATGTTCG AAAAGGCGGC AGCAGCGGCA GCGGTATCGC CCCCAGCTCC CATGCAGATT
    GCATCCGGTT CCCCAGCTTC GGGAGGCGGA ACTCGACGAT CCGAGGCGGA GCAGCAGGAA
    ATGGACAAGT CTTTCGAAGA TTCAATACAA GCACTAAATA ATTTAATTGG CGAACTAGAC
    TCGTTCCAAC GCGAGATCGA TGAGGGCAAG GTCAAACCGC CGAGCAACAT CATAAGCGGC
    AGCACCACCA GTAGCAACAA CAACAATACG ACGACCAGCA GCATCAGCAG CAGCGACAAC
    AACAACCTGC CCGCCACCAG CAACATCGAG CCATGCGCCA TCAGCAATCA GACGAACTCG
    AGCGGCTGCG GCACGGACAT ATCCGACACC ACGTCCGACG AACTGGCCGG CGACGATATG
    GACGTCAGGC GGCGGGATCG GGATCGGGAC CGGGATCTGC TGGGCGCCAG CGATTCGGAG
    CTGAGTCGCT GCTATGTGAG CGAGACGAGT TCGCTGACCG GTGGCCTAAC GGCCGGCGGC
    TACGAGAATC CCACTTTCGC GCACTTTGCG GCCAATGCCA ATCGGGAGGA CGCTGTTTCG
    CTGGCCTCCG ACAGCGTTTG TCTCGGCCAG CCACGCCACG CCTATGTGGA TACCTGCAGC
    GACAGCGGCA GTGCCGTGGT GGTGATCTAC GACCACCAGA TTCCCAACAC ACCCGACATT
    GAGTTCGTGA AGCAGAACTC TGAGATTGTA GTGCTGCGGA CTAAGGATCC GCAGCCCCAC
    GCGCTGCAGC TGCACGAGAT GCGCGAGCTG CAGCAGTTGC CCGCCAATTT GGCCGGTTCG
    CCGGACTCCT CGCCGGACTC TGCCGGTGGC CAGGCACCGC CAACAGCAAC TGTGGCGCCC
    GCCAAGCAGC GACTCTCCTC GTTTCGCGCC ACCAGTGAGC AGCAGTTGCA GCTCCTCGGA
    CGCGGTAGCC CGCAAAGAGG TAAAACACCC AGTGAGCAGG CGGTACAAAG CAGGCCACAG
    GACCAGCATT TTCCACAGAC ACAACAGCAG GATATTGATG GCAGTAGTCC ACCAGTAGAA
    CTTGCAAGGC GCCAGCTGCC CCCCAAGCCC ACCAGTTTGA GTATTTTTAA CGGCCCCGTG
    CCCACTGCGG GCGATAGGCC TGTCGTGCCG CGAAAGTCGG ACTTTAAGGC TGATCTAGAT
    GCTAAAATAC GCAGGCAAAA GCAGAAGGTT AAACAGCAGT TGCAGCAGCA GCAGCAGCAA
    CAGCAGCAAG AGCAGCAAGA AACGCAGCAG CAGCAGCAAG CACCACAAGA ACAGCAACAC
    TCACCACAGT CGCCCCAAAC CAGAAACTGT AATGTCACTA ATCAACAAGC CGCCAATATT
    ACTGCATCAG CATCTGCATT TGCAACCGCA ACAGCATCCA CAGACCCGTA CCCGAATCCA
    AATCATAGAA TGCCAAACCA AAATCAGACA GCCACATCCA ATCACACGCA GTGCAAGACG
    CCCACAATGG CATTGTCACC GTCATCACCT CGCGGCCATT TGCCATTATC ATCGTCATCG
    CTATCGTCAT TACCATTACC AGCCACCACT TCATCACCAT CAAATGCCCG GCCATCGATG
    TTGCCCGCCA GTGACCGACC ACCCGCCCAT CCATATGTGT GCTCCAATGC CCCAGCCAAT
    CCCCACCACG CCAATAGCAT TTCCAATGCC AATGCCCATC TCAAGCCGTG CATTACGCCC
    CGGCCGGCTT CTTTGTCGGG AGGAGCAGCC GGCGGTGGCT CCACGCGCAT CGGGCGTCGA
    TCGTCCATTA ATCAGTCCAA GCCACCGCCG CCAGTCAGAC GCAGTTCGTC GGTGACCCCC
    AGTCCCAATG CCTCGGTCGG GCCGGACCCT CGCATCTGTC ACGAATCGCT GATGGATCAA
    ATCAAGCGCG GAGCCACCTT AAAGCGTAAC CAGAAGATCA ACGATCGCAG CGCTCCCAAG
    ATACATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12126
    Clone ID Related Accession (Same CDS sequence) NM_001259230.2
    Accession Version NM_001259230.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5607bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product missing-in-metastasis, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259230.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG AGCTGTCCGC AGCGCATCAT
    GCACAGCAGC AGCAGCAGCA GCAAAATCAG CAGCCCCAGA CGCCGCCCAC CTATGTGAAC
    ATGTCGGAGC TGGCCACCAT GGCCGCTTTG AAGCAGACCA ATCAGCAGCA GAAGCCCTCT
    ACGCCGCCTC TGCAGCAGCA GAGCTCCATT GACTCGACCA GCTCCCAGCA TTCCAACGAC
    TCCACCGGCT CTCATCAGCT CCTCCAGCAG CAGCAGCAGC ATCATCAATC GCAGCAGAAT
    CACCACTCAG CCACTGCCAC ACGCTCCCAT TCCATATCCT CGACGGCCTC GTCACTTCAC
    TCGCATCCGT CGATCGACTC CACCGTCGCT TGCGGCTCGC TGGTGGGCCA GCACAACCAC
    AGCACCAGCA CCAACACGAA CACCACCTCG CCGTCCAGTG GCAGCTCCAC GCCACAGAAC
    CATTACTCGC CCCTGCTAAC CAACTCCCCC ACGTCCACTG CCGCAGGTAC TCCCAGTGGC
    AGCAGCTTAG GTCCTGGGTC CGGTTTGGGA TTTGTCTACC AGGTCAGCTC CCCGACGCCT
    CCCTCCAGCG AGGTGCTGAA AATCACCGAG CAAGCCGCTG CAGGACAGGA TCAGGGTCCA
    GCCAACAGCG TAGCGGACGA GACGGATGAG CGATCAAGGG CCTCAGTCCT GCAGAAGGCT
    TCAATGTTCG AAAAGGCGGC AGCAGCGGCA GCGGTATCGC CCCCAGCTCC CATGCAGATT
    GCATCCGGTT CCCCAGCTTC GGGAGGCGGA ACTCGACGAT CCGAGGCGGA GCAGCAGGAA
    ATGGACAAGT CTTTCGAAGA TTCAATACAA GCACTAAATA ATTTAATTGG CGAACTAGAC
    TCGTTCCAAC GCGAGATCGA TGAGGGCAAG GTCAAACCGC CGAGCAACAT CATAAGCGGC
    AGCACCACCA GTAGCAACAA CAACAATACG ACGACCAGCA GCATCAGCAG CAGCGACAAC
    AACAACCTGC CCGCCACCAG CAACATCGAG CCATGCGCCA TCAGCAATCA GACGAACTCG
    AGCGGCTGCG GCACGGACAT ATCCGACACC ACGTCCGACG AACTGGCCGG CGACGATATG
    GACGTCAGGC GGCGGGATCG GGATCGGGAC CGGGATCTGC TGGGCGCCAG CGATTCGGAG
    CTGAGTCGCT GCTATGTGAG CGAGACGAGT TCGCTGACCG GTGGCCTAAC GGCCGGCGGC
    TACGAGAATC CCACTTTCGC GCACTTTGCG GCCAATGCCA ATCGGGAGGA CGCTGTTTCG
    CTGGCCTCCG ACAGCGTTTG TCTCGGCCAG CCACGCCACG CCTATGTGGA TACCTGCAGC
    GACAGCGGCA GTGCCGTGGT GGTGATCTAC GACCACCAGA TTCCCAACAC ACCCGACATT
    GAGTTCGTGA AGCAGAACTC TGAGATTGTA GTGCTGCGGA CTAAGGATCC GCAGCCCCAC
    GCGCTGCAGC TGCACGAGAT GCGCGAGCTG CAGCAGTTGC CCGCCAATTT GGCCGGTTCG
    CCGGACTCCT CGCCGGACTC TGCCGGTGGC CAGGCACCGC CAACAGCAAC TGTGGCGCCC
    GCCAAGCAGC GACTCTCCTC GTTTCGCGCC ACCAGTGAGC AGCAGTTGCA GCTCCTCGGA
    CGCGGTAGCC CGCAAAGAGG TAAAACACCC AGTGAGCAGG CGGTACAAAG CAGGCCACAG
    GACCAGCATT TTCCACAGAC ACAACAGCAG GATATTGATG GCAGTAGTCC ACCAGTAGAA
    CTTGCAAGGC GCCAGCTGCC CCCCAAGCCC ACCAGTTTGA GTATTTTTAA CGGCCCCGTG
    CCCACTGCGG GCGATAGGCC TGTCGTGCCG CGAAAGTCGG ACTTTAAGGC TGATCTAGAT
    GCTAAAATAC GCAGGCAAAA GCAGAAGGTT AAACAGCAGT TGCAGCAGCA GCAGCAGCAA
    CAGCAGCAAG AGCAGCAAGA AACGCAGCAG CAGCAGCAAG CACCACAAGA ACAGCAACAC
    TCACCACAGT CGCCCCAAAC CAGAAACTGT AATGTCACTA ATCAACAAGC CGCCAATATT
    ACTGCATCAG CATCTGCATT TGCAACCGCA ACAGCATCCA CAGACCCGTA CCCGAATCCA
    AATCATAGAA TGCCAAACCA AAATCAGACA GCCACATCCA ATCACACGCA GTGCAAGACG
    CCCACAATGG CATTGTCACC GTCATCACCT CGCGGCCATT TGCCATTATC ATCGTCATCG
    CTATCGTCAT TACCATTACC AGCCACCACT TCATCACCAT CAAATGCCCG GCCATCGATG
    TTGCCCGCCA GTGACCGACC ACCCGCCCAT CCATATGTGT GCTCCAATGC CCCAGCCAAT
    CCCCACCACG CCAATAGCAT TTCCAATGCC AATGCCCATC TCAAGCCGTG CATTACGCCC
    CGGCCGGCTT CTTTGTCGGG AGGAGCAGCC GGCGGTGGCT CCACGCGCAT CGGGCGTCGA
    TCGTCCATTA ATCAGTCCAA GCCACCGCCG CCAGTCAGAC GCAGTTCGTC GGTGACCCCC
    AGTCCCAATG CCTCGGTCGG GCTGCAGCAG CAACCACAGC ACGCGACTCT GTCGCAGCAG
    AATCACCAAC TAAGCAGCTC CAGCGAGCAC TTACCGCCGC CACCGCCATT CATGCTGGAC
    GCCATGCCCC AGATTCCCAG CTCAGCGCTG AAGGTATCGG AGACGGTAAG AGCCCTGGCA
    GCCATGCGGC ACCAGCCAGC ATCACCTGTG TCCCTCAGAC GCATGCAGCA GCAACAGCAG
    CAGCAGCTTC AGCAGCAACA GCAGCAACAG GTGCAGCAGC AGCAACCCCT ATTGCAGACG
    TTCCGCACCT CATCACCAGC CGCGGGCGGA GGGGGTGGCG GCGGCATATA CGCCCAGCCC
    AAACTGGTCA ACAGCATGTC CAGCTTCCGC ACCAGCAGCC CCAGTCCCAA CGGACATGCT
    CACCCACTGC CACCGACACA GCCAAAGGCG AATCCGAACC TAATTGCACA GCTCAATGCA
    CGACTCAGCG GCAAACAGCA GCAGCACCAA CAGCAGCAGC ATATCGAGGG GATCTACGGC
    AACCAGCAGG CGCCCGGAGG AGAGTCGATC TACATGCGGA GTGGCCTGTC CATGTCGCAG
    CCGCAACAGC AGCAACACTT TGACGCTGCG CAATCGCCGA ACATGAGACA GGCTCATTCC
    CATCAGCACC ACCAGCCGCC GCAGCAGCAG CACTACACCT GTCCGCCTCC TCTGGAGGAT
    CCCCCACCGC CGCCCATTTA CGCCGCCGGT GCATCGGCCA CGATGCCCAA GAAGATGGCC
    CGTCCGCCCA CTGGCCAGAA CGCGACTCAC TCGAGCGCCT ATGCAGCAGC CTCGTCTACG
    GCCACGCTGC CCAAGAATAT GATGCAGCAG CAGCAGCGGT TGCAGCACCA GCAGCAGTAT
    CAACAGCCGG CAGGCATGGG CATTGGCAAC GGCAATGGGC ACCTAGGTCA GCGTCCGCAG
    TTGCCGCTGC CCCAGCAGAA GCTTAGAGCT GCACAGCAGC AGCACTTGGC GGAGCAGCAG
    CATCAGCAAC AGCAAGAACA GCAGCAGCAC CACCAGCAGC ACCAACAACA CCAGCAACGC
    CAGCCGCCCA TTCCTTCACG CCACTCAAGT GTGCAGCAAA AGATATTCGT ATCAACGAAT
    CCATTCATAC AGACAACGGC CGTCAAGTTT CACTCGCCCT CGGCCTCGCC CACGTGCGGC
    TCGCCCGTTA CTGGGTCCTT GGCTAGCATT TATGCCACAA CCTCGCGTGG CGGCCACCAT
    CACCAGCAGC AGCAGGCTCA GCAGCAGCAG CACTACTATC GCGATGCTGC CGGGGGCAAC
    AGCAACGGCG GCGCTGCCTA CTATAACCAC AATGCCCATG CCCATTCCCA GGCACATCAT
    CCAAACTATG CCACAAGCAC AAATATCGAA AAGACTGGCA GTATTCGTGC CAAGACCAAG
    GCCGAATTCC TCGAGAATCT CAACGCGAAG CTGGCGAAGC AGGGAATGTC TGGACGAGCA
    TTTGCCGTGC GAAATCTCAT CAACAGCAAG GCCCTGATGT ATCAAAATCC GCAAAAACTA
    TCGCGACCCA GTGCGCAATA CCGTAGACCA CCCACCTATC CCAACACCAG CACAACCACC
    AATGCCACTT GCGAAGATCA GTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246159.1
    CDS890..6496
    Translation

    Target ORF information:

    RefSeq Version NM_001259230.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster missing-in-metastasis (mim), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259230.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG AGCTGTCCGC AGCGCATCAT
    GCACAGCAGC AGCAGCAGCA GCAAAATCAG CAGCCCCAGA CGCCGCCCAC CTATGTGAAC
    ATGTCGGAGC TGGCCACCAT GGCCGCTTTG AAGCAGACCA ATCAGCAGCA GAAGCCCTCT
    ACGCCGCCTC TGCAGCAGCA GAGCTCCATT GACTCGACCA GCTCCCAGCA TTCCAACGAC
    TCCACCGGCT CTCATCAGCT CCTCCAGCAG CAGCAGCAGC ATCATCAATC GCAGCAGAAT
    CACCACTCAG CCACTGCCAC ACGCTCCCAT TCCATATCCT CGACGGCCTC GTCACTTCAC
    TCGCATCCGT CGATCGACTC CACCGTCGCT TGCGGCTCGC TGGTGGGCCA GCACAACCAC
    AGCACCAGCA CCAACACGAA CACCACCTCG CCGTCCAGTG GCAGCTCCAC GCCACAGAAC
    CATTACTCGC CCCTGCTAAC CAACTCCCCC ACGTCCACTG CCGCAGGTAC TCCCAGTGGC
    AGCAGCTTAG GTCCTGGGTC CGGTTTGGGA TTTGTCTACC AGGTCAGCTC CCCGACGCCT
    CCCTCCAGCG AGGTGCTGAA AATCACCGAG CAAGCCGCTG CAGGACAGGA TCAGGGTCCA
    GCCAACAGCG TAGCGGACGA GACGGATGAG CGATCAAGGG CCTCAGTCCT GCAGAAGGCT
    TCAATGTTCG AAAAGGCGGC AGCAGCGGCA GCGGTATCGC CCCCAGCTCC CATGCAGATT
    GCATCCGGTT CCCCAGCTTC GGGAGGCGGA ACTCGACGAT CCGAGGCGGA GCAGCAGGAA
    ATGGACAAGT CTTTCGAAGA TTCAATACAA GCACTAAATA ATTTAATTGG CGAACTAGAC
    TCGTTCCAAC GCGAGATCGA TGAGGGCAAG GTCAAACCGC CGAGCAACAT CATAAGCGGC
    AGCACCACCA GTAGCAACAA CAACAATACG ACGACCAGCA GCATCAGCAG CAGCGACAAC
    AACAACCTGC CCGCCACCAG CAACATCGAG CCATGCGCCA TCAGCAATCA GACGAACTCG
    AGCGGCTGCG GCACGGACAT ATCCGACACC ACGTCCGACG AACTGGCCGG CGACGATATG
    GACGTCAGGC GGCGGGATCG GGATCGGGAC CGGGATCTGC TGGGCGCCAG CGATTCGGAG
    CTGAGTCGCT GCTATGTGAG CGAGACGAGT TCGCTGACCG GTGGCCTAAC GGCCGGCGGC
    TACGAGAATC CCACTTTCGC GCACTTTGCG GCCAATGCCA ATCGGGAGGA CGCTGTTTCG
    CTGGCCTCCG ACAGCGTTTG TCTCGGCCAG CCACGCCACG CCTATGTGGA TACCTGCAGC
    GACAGCGGCA GTGCCGTGGT GGTGATCTAC GACCACCAGA TTCCCAACAC ACCCGACATT
    GAGTTCGTGA AGCAGAACTC TGAGATTGTA GTGCTGCGGA CTAAGGATCC GCAGCCCCAC
    GCGCTGCAGC TGCACGAGAT GCGCGAGCTG CAGCAGTTGC CCGCCAATTT GGCCGGTTCG
    CCGGACTCCT CGCCGGACTC TGCCGGTGGC CAGGCACCGC CAACAGCAAC TGTGGCGCCC
    GCCAAGCAGC GACTCTCCTC GTTTCGCGCC ACCAGTGAGC AGCAGTTGCA GCTCCTCGGA
    CGCGGTAGCC CGCAAAGAGG TAAAACACCC AGTGAGCAGG CGGTACAAAG CAGGCCACAG
    GACCAGCATT TTCCACAGAC ACAACAGCAG GATATTGATG GCAGTAGTCC ACCAGTAGAA
    CTTGCAAGGC GCCAGCTGCC CCCCAAGCCC ACCAGTTTGA GTATTTTTAA CGGCCCCGTG
    CCCACTGCGG GCGATAGGCC TGTCGTGCCG CGAAAGTCGG ACTTTAAGGC TGATCTAGAT
    GCTAAAATAC GCAGGCAAAA GCAGAAGGTT AAACAGCAGT TGCAGCAGCA GCAGCAGCAA
    CAGCAGCAAG AGCAGCAAGA AACGCAGCAG CAGCAGCAAG CACCACAAGA ACAGCAACAC
    TCACCACAGT CGCCCCAAAC CAGAAACTGT AATGTCACTA ATCAACAAGC CGCCAATATT
    ACTGCATCAG CATCTGCATT TGCAACCGCA ACAGCATCCA CAGACCCGTA CCCGAATCCA
    AATCATAGAA TGCCAAACCA AAATCAGACA GCCACATCCA ATCACACGCA GTGCAAGACG
    CCCACAATGG CATTGTCACC GTCATCACCT CGCGGCCATT TGCCATTATC ATCGTCATCG
    CTATCGTCAT TACCATTACC AGCCACCACT TCATCACCAT CAAATGCCCG GCCATCGATG
    TTGCCCGCCA GTGACCGACC ACCCGCCCAT CCATATGTGT GCTCCAATGC CCCAGCCAAT
    CCCCACCACG CCAATAGCAT TTCCAATGCC AATGCCCATC TCAAGCCGTG CATTACGCCC
    CGGCCGGCTT CTTTGTCGGG AGGAGCAGCC GGCGGTGGCT CCACGCGCAT CGGGCGTCGA
    TCGTCCATTA ATCAGTCCAA GCCACCGCCG CCAGTCAGAC GCAGTTCGTC GGTGACCCCC
    AGTCCCAATG CCTCGGTCGG GCTGCAGCAG CAACCACAGC ACGCGACTCT GTCGCAGCAG
    AATCACCAAC TAAGCAGCTC CAGCGAGCAC TTACCGCCGC CACCGCCATT CATGCTGGAC
    GCCATGCCCC AGATTCCCAG CTCAGCGCTG AAGGTATCGG AGACGGTAAG AGCCCTGGCA
    GCCATGCGGC ACCAGCCAGC ATCACCTGTG TCCCTCAGAC GCATGCAGCA GCAACAGCAG
    CAGCAGCTTC AGCAGCAACA GCAGCAACAG GTGCAGCAGC AGCAACCCCT ATTGCAGACG
    TTCCGCACCT CATCACCAGC CGCGGGCGGA GGGGGTGGCG GCGGCATATA CGCCCAGCCC
    AAACTGGTCA ACAGCATGTC CAGCTTCCGC ACCAGCAGCC CCAGTCCCAA CGGACATGCT
    CACCCACTGC CACCGACACA GCCAAAGGCG AATCCGAACC TAATTGCACA GCTCAATGCA
    CGACTCAGCG GCAAACAGCA GCAGCACCAA CAGCAGCAGC ATATCGAGGG GATCTACGGC
    AACCAGCAGG CGCCCGGAGG AGAGTCGATC TACATGCGGA GTGGCCTGTC CATGTCGCAG
    CCGCAACAGC AGCAACACTT TGACGCTGCG CAATCGCCGA ACATGAGACA GGCTCATTCC
    CATCAGCACC ACCAGCCGCC GCAGCAGCAG CACTACACCT GTCCGCCTCC TCTGGAGGAT
    CCCCCACCGC CGCCCATTTA CGCCGCCGGT GCATCGGCCA CGATGCCCAA GAAGATGGCC
    CGTCCGCCCA CTGGCCAGAA CGCGACTCAC TCGAGCGCCT ATGCAGCAGC CTCGTCTACG
    GCCACGCTGC CCAAGAATAT GATGCAGCAG CAGCAGCGGT TGCAGCACCA GCAGCAGTAT
    CAACAGCCGG CAGGCATGGG CATTGGCAAC GGCAATGGGC ACCTAGGTCA GCGTCCGCAG
    TTGCCGCTGC CCCAGCAGAA GCTTAGAGCT GCACAGCAGC AGCACTTGGC GGAGCAGCAG
    CATCAGCAAC AGCAAGAACA GCAGCAGCAC CACCAGCAGC ACCAACAACA CCAGCAACGC
    CAGCCGCCCA TTCCTTCACG CCACTCAAGT GTGCAGCAAA AGATATTCGT ATCAACGAAT
    CCATTCATAC AGACAACGGC CGTCAAGTTT CACTCGCCCT CGGCCTCGCC CACGTGCGGC
    TCGCCCGTTA CTGGGTCCTT GGCTAGCATT TATGCCACAA CCTCGCGTGG CGGCCACCAT
    CACCAGCAGC AGCAGGCTCA GCAGCAGCAG CACTACTATC GCGATGCTGC CGGGGGCAAC
    AGCAACGGCG GCGCTGCCTA CTATAACCAC AATGCCCATG CCCATTCCCA GGCACATCAT
    CCAAACTATG CCACAAGCAC AAATATCGAA AAGACTGGCA GTATTCGTGC CAAGACCAAG
    GCCGAATTCC TCGAGAATCT CAACGCGAAG CTGGCGAAGC AGGGAATGTC TGGACGAGCA
    TTTGCCGTGC GAAATCTCAT CAACAGCAAG GCCCTGATGT ATCAAAATCC GCAAAAACTA
    TCGCGACCCA GTGCGCAATA CCGTAGACCA CCCACCTATC CCAACACCAG CACAACCACC
    AATGCCACTT GCGAAGATCA GTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12127
    Clone ID Related Accession (Same CDS sequence) NM_001259229.2
    Accession Version NM_001259229.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5805bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product missing-in-metastasis, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259229.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG AGCTGTCCGC AGCGCATCAT
    GCACAGCAGC AGCAGCAGCA GCAAAATCAG CAGCCCCAGA CGCCGCCCAC CTATGTGAAC
    ATGTCGGAGC TGGCCACCAT GGCCGCTTTG AAGCAGACCA ATCAGCAGCA GAAGCCCTCT
    ACGCCGCCTC TGCAGCAGCA GAGCTCCATT GACTCGACCA GCTCCCAGCA TTCCAACGAC
    TCCACCGGCT CTCATCAGCT CCTCCAGCAG CAGCAGCAGC ATCATCAATC GCAGCAGAAT
    CACCACTCAG CCACTGCCAC ACGCTCCCAT TCCATATCCT CGACGGCCTC GTCACTTCAC
    TCGCATCCGT CGATCGACTC CACCGTCGCT TGCGGCTCGC TGGTGGGCCA GCACAACCAC
    AGCACCAGCA CCAACACGAA CACCACCTCG CCGTCCAGTG GCAGCTCCAC GCCACAGAAC
    CATTACTCGC CCCTGCTAAC CAACTCCCCC ACGTCCACTG CCGCAGGTAC TCCCAGTGGC
    AGCAGCTTAG GTCCTGGGTC CGGTTTGGGA TTTGTCTACC AGGTCAGCTC CCCGACGCCT
    CCCTCCAGCG AGGTGCTGAA AATCACCGAG CAAGCCGCTG CAGGACAGGA TCAGGGTCCA
    GCCAACAGCG TAGCGGACGA GACGGATGAG CGATCAAGGG CCTCAGTCCT GCAGAAGGCT
    TCAATGTTCG AAAAGGCGGC AGCAGCGGCA GCGGTATCGC CCCCAGCTCC CATGCAGATT
    GCATCCGGTT CCCCAGCTTC GGGAGGCGGA ACTCGACGAT CCGAGGCGGA GCAGCAGGAA
    ATGGACAAGT CTTTCGAAGA TTCAATACAA GCACTAAATA ATTTAATTGG CGAACTAGAC
    TCGTTCCAAC GCGAGATCGA TGAGGGCAAG GTCAAACCGC CGAGCAACAT CATAAGCGGC
    AGCACCACCA GTAGCAACAA CAACAATACG ACGACCAGCA GCATCAGCAG CAGCGACAAC
    AACAACCTGC CCGCCACCAG CAACATCGAG CCATGCGCCA TCAGCAATCA GACGAACTCG
    AGCGGCTGCG GCACGGACAT ATCCGACACC ACGTCCGACG AACTGGCCGG CGACGATATG
    GACGTCAGGC GGCGGGATCG GGATCGGGAC CGGGATCTGC TGGGCGCCAG CGATTCGGAG
    CTGAGTCGCT GCTATGTGAG CGAGACGAGT TCGCTGACCG GTGGCCTAAC GGCCGGCGGC
    TACGAGAATC CCACTTTCGC GCACTTTGCG GCCAATGCCA ATCGGGAGGA CGCTGTTTCG
    CTGGCCTCCG ACAGCGTTTG TCTCGGCCAG CCACGCCACG CCTATGTGGA TACCTGCAGC
    GACAGCGGCA GTGCCGTGGT GGTGATCTAC GACCACCAGA TTCCCAACAC ACCCGACATT
    GAGTTCGTGA AGCAGAACTC TGAGATTGTA GTGCTGCGGA CTAAGGATCC GCAGCCCCAC
    GCGCTGCAGC TGCACGAGAT GCGCGAGCTG CAGCAGTTGC CCGCCAATTT GGCCGGTTCG
    CCGGACTCCT CGCCGGACTC TGCCGGTGGC CAGGCACCGC CAACAGCAAC TGTGGCGCCC
    GCCAAGCAGC GACTCTCCTC GTTTCGCGCC ACCAGTGAGC AGCAGTTGCA GCTCCTCGGA
    CGCGGTAGCC CGCAAAGAGG TAAAACACCC AGTGAGCAGG CGGTACAAAG CAGGCCACAG
    GACCAGCATT TTCCACAGAC ACAACAGCAG GATATTGATG GCAGTAGTCC ACCAGTAGAA
    CTTGCAAGGC GCCAGCTGCC CCCCAAGCCC ACCAGTTTGA GTATTTTTAA CGGCCCCGTG
    CCCACTGCGG GCGATAGGCC TGTCGTGCCG CGAAAGTCGG ACTTTAAGGC TGATCTAGAT
    GCTAAAATAC GCAGGCAAAA GCAGAAGGTT AAACAGCAGT TGCAGCAGCA GCAGCAGCAA
    CAGCAGCAAG AGCAGCAAGA AACGCAGCAG CAGCAGCAAG CACCACAAGA ACAGCAACAC
    TCACCACAGT CGCCCCAAAC CAGAAACTGT AATGTCACTA ATCAACAAGC CGCCAATATT
    ACTGCATCAG CATCTGCATT TGCAACCGCA ACAGCATCCA CAGACCCGTA CCCGAATCCA
    AATCATAGAA TGCCAAACCA AAATCAGACA GCCACATCCA ATCACACGCA GTGCAAGACG
    CCCACAATGG CATTGTCACC GTCATCACCT CGCGGCCATT TGCCATTATC ATCGTCATCG
    CTATCGTCAT TACCATTACC AGCCACCACT TCATCACCAT CAAATGCCCG GCCATCGATG
    TTGCCCGCCA GTGACCGACC ACCCGCCCAT CCATATGTGT GCTCCAATGC CCCAGCCAAT
    CCCCACCACG CCAATAGCAT TTCCAATGCC AATGCCCATC TCAAGCCGTG CATTACGCCC
    CGGCCGGCTT CTTTGTCGGG AGGAGCAGCC GGCGGTGGCT CCACGCGCAT CGGGCGTCGA
    TCGTCCATTA ATCAGTCCAA GCCACCGCCG CCAGTCAGAC GCAGTTCGTC GGTGACCCCC
    AGTCCCAATG CCTCGGTCGG GCTGCAGCAG CAACCACAGC ACGCGACTCT GTCGCAGCAG
    AATCACCAAC TAAGCAGCTC CAGCGAGCAC TTACCGCCGC CACCGCCATT CATGCTGGAC
    GCCATGCCCC AGATTCCCAG CTCAGCGCTG AAGGTATCGG AGACGGTAAG AGCCCTGGCA
    GCCATGCGGC ACCAGCCAGC ATCACCTGTG TCCCTCAGAC GCATGCAGCA GCAACAGCAG
    CAGCAGCTTC AGCAGCAACA GCAGCAACAG GTGCAGCAGC AGCAACCCCT ATTGCAGTCT
    GCGCACAACT CCCCCCTCAA AGAGGACCTG ACCGTGTACT ACGACTCCTA CTTGGATCTG
    CACGCCTATG CCCAGGCCTT GGCCAGCGGC CAACAGCCCG GCGGTCAGCA GATGGCCAAC
    CAGCAACGCT TTACCCTCCA GCAGCAACAT CAGCATCTGC ATCAGCCACA GCAACCGCCT
    GTCTACCAAG TCGATGCCAC GTTCCGCACC TCATCACCAG CCGCGGGCGG AGGGGGTGGC
    GGCGGCATAT ACGCCCAGCC CAAACTGGTC AACAGCATGT CCAGCTTCCG CACCAGCAGC
    CCCAGTCCCA ACGGACATGC TCACCCACTG CCACCGACAC AGCCAAAGGC GAATCCGAAC
    CTAATTGCAC AGCTCAATGC ACGACTCAGC GGCAAACAGC AGCAGCACCA ACAGCAGCAG
    CATATCGAGG GGATCTACGG CAACCAGCAG GCGCCCGGAG GAGAGTCGAT CTACATGCGG
    AGTGGCCTGT CCATGTCGCA GCCGCAACAG CAGCAACACT TTGACGCTGC GCAATCGCCG
    AACATGAGAC AGGCTCATTC CCATCAGCAC CACCAGCCGC CGCAGCAGCA GCACTACACC
    TGTCCGCCTC CTCTGGAGGA TCCCCCACCG CCGCCCATTT ACGCCGCCGG TGCATCGGCC
    ACGATGCCCA AGAAGATGGC CCGTCCGCCC ACTGGCCAGA ACGCGACTCA CTCGAGCGCC
    TATGCAGCAG CCTCGTCTAC GGCCACGCTG CCCAAGAATA TGATGCAGCA GCAGCAGCGG
    TTGCAGCACC AGCAGCAGTA TCAACAGCCG GCAGGCATGG GCATTGGCAA CGGCAATGGG
    CACCTAGGTC AGCGTCCGCA GTTGCCGCTG CCCCAGCAGA AGCTTAGAGC TGCACAGCAG
    CAGCACTTGG CGGAGCAGCA GCATCAGCAA CAGCAAGAAC AGCAGCAGCA CCACCAGCAG
    CACCAACAAC ACCAGCAACG CCAGCCGCCC ATTCCTTCAC GCCACTCAAG TGTGCAGCAA
    AAGATATTCG TATCAACGAA TCCATTCATA CAGACAACGG CCGTCAAGTT TCACTCGCCC
    TCGGCCTCGC CCACGTGCGG CTCGCCCGTT ACTGGGTCCT TGGCTAGCAT TTATGCCACA
    ACCTCGCGTG GCGGCCACCA TCACCAGCAG CAGCAGGCTC AGCAGCAGCA GCACTACTAT
    CGCGATGCTG CCGGGGGCAA CAGCAACGGC GGCGCTGCCT ACTATAACCA CAATGCCCAT
    GCCCATTCCC AGGCACATCA TCCAAACTAT GCCACAAGCA CAAATATCGA AAAGACTGGC
    AGTATTCGTG CCAAGACCAA GGCCGAATTC CTCGAGAATC TCAACGCGAA GCTGGCGAAG
    CAGGGAATGT CTGGACGAGC ATTTGCCGTG CGAAATCTCA TCAACAGCAA GGCCCTGCCG
    GACCCTCGCA TCTGTCACGA ATCGCTGATG GATCAAATCA AGCGCGGAGC CACCTTAAAG
    CGTAACCAGA AGATCAACGA TCGCAGCGCT CCCAAGATAC ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246158.1
    CDS890..6694
    Translation

    Target ORF information:

    RefSeq Version NM_001259229.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster missing-in-metastasis (mim), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259229.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGATCTAA GTCTGGAACG CGATAGCTCC GCGCTGGGTA GTCTATTTCA GCAGATTATC 
    AATGACATGA AGAACACTTC GCCCCTGTGG GAGGATTTTG TGGCTAAGGC CGGAAAACTG
    CACACTTGTT TGAGGGCCGC CATCCAGGCA ATCGCCGCCT ATTTGGATGC CTTCCAAAAG
    ATAGCCGATG CGGCGACCAA CTCAAGAGGC GCCTCGAAGG AGATCGGCAC CGCCCTAACC
    CGCGTTTGCC TCCGCCACAA GGCGGTGGAG ACGCGTCTGA AGACCTTCAC CAGCACCATT
    ATGGATTGCC TGGTGCAGCC GCTGCAGGAG AGGATCGAGG ACTGGAAGCG CACGGTGGCC
    ACCATCGACA AAGACCATGC CAAAGAGTAT AAGCGCTGTC GCAGTGAACT GAAGAAGCGT
    TCCAGCGACA CACTGCGCCT GCAGAAGAAG GCGCGCAAGG GTCAGACGGA CGGATTGCAG
    TCCCTGATGG ACTCGCACAT GCAGGATGTT ACCCTGCGCC GGGCAGAATT GGAGGAAGTC
    GAGAAGAAAT CCTTGAGGGC GGCCATGGTG GAGGAGCGTC TGCGCTACTG CAGCTTCGTC
    CACATGCTTC AGCCAGTGGT GCACGAGGAG TGCGAGGTCA TGTCAGAGTT GGGTCACCTA
    CAGGAAGCCA TGCAGTCAAT TGCCCTAGTA ACCAAGGAGC CTAGTGTTCT GCCCCAGGCC
    TCCGAGGAGC TTATCCATGA CGCTAAGGCC AGCATTAATC TGTACCCGGA GTCCCCGGGT
    GGCGGTTCCG GCTCGCAGGG CGGCGGCTGC TCCAACTCGC TGGGTTCCCG AAAGAGCTCC
    GTCTGCTCCA TCAGCAGTAT GAACAGCAGC GGCTCGAGCA ACTCGCCCGG TCACCATCAC
    TATCCGCGCT CCCTTTCGCA GTTTGTAACG CCCGCAATTC GCTTGAAACC TGGTGAATCC
    AGTGATAGTG GCTTTTGCTC ATCGCCAGCT TTAACAACAC AGACTTCGAA TGCAACGAAC
    CAGACGGCAA ATGTCTCCAC CTGGCCCCCA CATTCCCAGG ATGGCGTCGA CACCCTGCCA
    CCGACCGCTG ACCGTCCGCA CACGATTTCG ACGGCATACG AAAAGGGTCA CCAGCGTCCG
    CCGCTGACCG TCTACACGTT CCAAAACCCG GAGACCATTC ACGAGTCGGG CAGCTGCCTG
    AACAACGGAA CAGCAGCCCC GAATGGACAG CCGTTATCCG GACAAGCCAC TCCGGCCACC
    CAGAAATCAC CGGCTGCCTC ACTTAGCCGG CCTCCTTTGC CAGTTCGCTG CTCGTCGTTG
    GAGCGACCCC TTTCGGCCCA GAGTAACCAC CGCCAGGGTA GCGGGAATAA CCTGCTGCAG
    CGCCAGTGCC CCTCGCCGAT TCCGGCTCAT ATCACGAAAG AGCTGTCCGC AGCGCATCAT
    GCACAGCAGC AGCAGCAGCA GCAAAATCAG CAGCCCCAGA CGCCGCCCAC CTATGTGAAC
    ATGTCGGAGC TGGCCACCAT GGCCGCTTTG AAGCAGACCA ATCAGCAGCA GAAGCCCTCT
    ACGCCGCCTC TGCAGCAGCA GAGCTCCATT GACTCGACCA GCTCCCAGCA TTCCAACGAC
    TCCACCGGCT CTCATCAGCT CCTCCAGCAG CAGCAGCAGC ATCATCAATC GCAGCAGAAT
    CACCACTCAG CCACTGCCAC ACGCTCCCAT TCCATATCCT CGACGGCCTC GTCACTTCAC
    TCGCATCCGT CGATCGACTC CACCGTCGCT TGCGGCTCGC TGGTGGGCCA GCACAACCAC
    AGCACCAGCA CCAACACGAA CACCACCTCG CCGTCCAGTG GCAGCTCCAC GCCACAGAAC
    CATTACTCGC CCCTGCTAAC CAACTCCCCC ACGTCCACTG CCGCAGGTAC TCCCAGTGGC
    AGCAGCTTAG GTCCTGGGTC CGGTTTGGGA TTTGTCTACC AGGTCAGCTC CCCGACGCCT
    CCCTCCAGCG AGGTGCTGAA AATCACCGAG CAAGCCGCTG CAGGACAGGA TCAGGGTCCA
    GCCAACAGCG TAGCGGACGA GACGGATGAG CGATCAAGGG CCTCAGTCCT GCAGAAGGCT
    TCAATGTTCG AAAAGGCGGC AGCAGCGGCA GCGGTATCGC CCCCAGCTCC CATGCAGATT
    GCATCCGGTT CCCCAGCTTC GGGAGGCGGA ACTCGACGAT CCGAGGCGGA GCAGCAGGAA
    ATGGACAAGT CTTTCGAAGA TTCAATACAA GCACTAAATA ATTTAATTGG CGAACTAGAC
    TCGTTCCAAC GCGAGATCGA TGAGGGCAAG GTCAAACCGC CGAGCAACAT CATAAGCGGC
    AGCACCACCA GTAGCAACAA CAACAATACG ACGACCAGCA GCATCAGCAG CAGCGACAAC
    AACAACCTGC CCGCCACCAG CAACATCGAG CCATGCGCCA TCAGCAATCA GACGAACTCG
    AGCGGCTGCG GCACGGACAT ATCCGACACC ACGTCCGACG AACTGGCCGG CGACGATATG
    GACGTCAGGC GGCGGGATCG GGATCGGGAC CGGGATCTGC TGGGCGCCAG CGATTCGGAG
    CTGAGTCGCT GCTATGTGAG CGAGACGAGT TCGCTGACCG GTGGCCTAAC GGCCGGCGGC
    TACGAGAATC CCACTTTCGC GCACTTTGCG GCCAATGCCA ATCGGGAGGA CGCTGTTTCG
    CTGGCCTCCG ACAGCGTTTG TCTCGGCCAG CCACGCCACG CCTATGTGGA TACCTGCAGC
    GACAGCGGCA GTGCCGTGGT GGTGATCTAC GACCACCAGA TTCCCAACAC ACCCGACATT
    GAGTTCGTGA AGCAGAACTC TGAGATTGTA GTGCTGCGGA CTAAGGATCC GCAGCCCCAC
    GCGCTGCAGC TGCACGAGAT GCGCGAGCTG CAGCAGTTGC CCGCCAATTT GGCCGGTTCG
    CCGGACTCCT CGCCGGACTC TGCCGGTGGC CAGGCACCGC CAACAGCAAC TGTGGCGCCC
    GCCAAGCAGC GACTCTCCTC GTTTCGCGCC ACCAGTGAGC AGCAGTTGCA GCTCCTCGGA
    CGCGGTAGCC CGCAAAGAGG TAAAACACCC AGTGAGCAGG CGGTACAAAG CAGGCCACAG
    GACCAGCATT TTCCACAGAC ACAACAGCAG GATATTGATG GCAGTAGTCC ACCAGTAGAA
    CTTGCAAGGC GCCAGCTGCC CCCCAAGCCC ACCAGTTTGA GTATTTTTAA CGGCCCCGTG
    CCCACTGCGG GCGATAGGCC TGTCGTGCCG CGAAAGTCGG ACTTTAAGGC TGATCTAGAT
    GCTAAAATAC GCAGGCAAAA GCAGAAGGTT AAACAGCAGT TGCAGCAGCA GCAGCAGCAA
    CAGCAGCAAG AGCAGCAAGA AACGCAGCAG CAGCAGCAAG CACCACAAGA ACAGCAACAC
    TCACCACAGT CGCCCCAAAC CAGAAACTGT AATGTCACTA ATCAACAAGC CGCCAATATT
    ACTGCATCAG CATCTGCATT TGCAACCGCA ACAGCATCCA CAGACCCGTA CCCGAATCCA
    AATCATAGAA TGCCAAACCA AAATCAGACA GCCACATCCA ATCACACGCA GTGCAAGACG
    CCCACAATGG CATTGTCACC GTCATCACCT CGCGGCCATT TGCCATTATC ATCGTCATCG
    CTATCGTCAT TACCATTACC AGCCACCACT TCATCACCAT CAAATGCCCG GCCATCGATG
    TTGCCCGCCA GTGACCGACC ACCCGCCCAT CCATATGTGT GCTCCAATGC CCCAGCCAAT
    CCCCACCACG CCAATAGCAT TTCCAATGCC AATGCCCATC TCAAGCCGTG CATTACGCCC
    CGGCCGGCTT CTTTGTCGGG AGGAGCAGCC GGCGGTGGCT CCACGCGCAT CGGGCGTCGA
    TCGTCCATTA ATCAGTCCAA GCCACCGCCG CCAGTCAGAC GCAGTTCGTC GGTGACCCCC
    AGTCCCAATG CCTCGGTCGG GCTGCAGCAG CAACCACAGC ACGCGACTCT GTCGCAGCAG
    AATCACCAAC TAAGCAGCTC CAGCGAGCAC TTACCGCCGC CACCGCCATT CATGCTGGAC
    GCCATGCCCC AGATTCCCAG CTCAGCGCTG AAGGTATCGG AGACGGTAAG AGCCCTGGCA
    GCCATGCGGC ACCAGCCAGC ATCACCTGTG TCCCTCAGAC GCATGCAGCA GCAACAGCAG
    CAGCAGCTTC AGCAGCAACA GCAGCAACAG GTGCAGCAGC AGCAACCCCT ATTGCAGTCT
    GCGCACAACT CCCCCCTCAA AGAGGACCTG ACCGTGTACT ACGACTCCTA CTTGGATCTG
    CACGCCTATG CCCAGGCCTT GGCCAGCGGC CAACAGCCCG GCGGTCAGCA GATGGCCAAC
    CAGCAACGCT TTACCCTCCA GCAGCAACAT CAGCATCTGC ATCAGCCACA GCAACCGCCT
    GTCTACCAAG TCGATGCCAC GTTCCGCACC TCATCACCAG CCGCGGGCGG AGGGGGTGGC
    GGCGGCATAT ACGCCCAGCC CAAACTGGTC AACAGCATGT CCAGCTTCCG CACCAGCAGC
    CCCAGTCCCA ACGGACATGC TCACCCACTG CCACCGACAC AGCCAAAGGC GAATCCGAAC
    CTAATTGCAC AGCTCAATGC ACGACTCAGC GGCAAACAGC AGCAGCACCA ACAGCAGCAG
    CATATCGAGG GGATCTACGG CAACCAGCAG GCGCCCGGAG GAGAGTCGAT CTACATGCGG
    AGTGGCCTGT CCATGTCGCA GCCGCAACAG CAGCAACACT TTGACGCTGC GCAATCGCCG
    AACATGAGAC AGGCTCATTC CCATCAGCAC CACCAGCCGC CGCAGCAGCA GCACTACACC
    TGTCCGCCTC CTCTGGAGGA TCCCCCACCG CCGCCCATTT ACGCCGCCGG TGCATCGGCC
    ACGATGCCCA AGAAGATGGC CCGTCCGCCC ACTGGCCAGA ACGCGACTCA CTCGAGCGCC
    TATGCAGCAG CCTCGTCTAC GGCCACGCTG CCCAAGAATA TGATGCAGCA GCAGCAGCGG
    TTGCAGCACC AGCAGCAGTA TCAACAGCCG GCAGGCATGG GCATTGGCAA CGGCAATGGG
    CACCTAGGTC AGCGTCCGCA GTTGCCGCTG CCCCAGCAGA AGCTTAGAGC TGCACAGCAG
    CAGCACTTGG CGGAGCAGCA GCATCAGCAA CAGCAAGAAC AGCAGCAGCA CCACCAGCAG
    CACCAACAAC ACCAGCAACG CCAGCCGCCC ATTCCTTCAC GCCACTCAAG TGTGCAGCAA
    AAGATATTCG TATCAACGAA TCCATTCATA CAGACAACGG CCGTCAAGTT TCACTCGCCC
    TCGGCCTCGC CCACGTGCGG CTCGCCCGTT ACTGGGTCCT TGGCTAGCAT TTATGCCACA
    ACCTCGCGTG GCGGCCACCA TCACCAGCAG CAGCAGGCTC AGCAGCAGCA GCACTACTAT
    CGCGATGCTG CCGGGGGCAA CAGCAACGGC GGCGCTGCCT ACTATAACCA CAATGCCCAT
    GCCCATTCCC AGGCACATCA TCCAAACTAT GCCACAAGCA CAAATATCGA AAAGACTGGC
    AGTATTCGTG CCAAGACCAA GGCCGAATTC CTCGAGAATC TCAACGCGAA GCTGGCGAAG
    CAGGGAATGT CTGGACGAGC ATTTGCCGTG CGAAATCTCA TCAACAGCAA GGCCCTGCCG
    GACCCTCGCA TCTGTCACGA ATCGCTGATG GATCAAATCA AGCGCGGAGC CACCTTAAAG
    CGTAACCAGA AGATCAACGA TCGCAGCGCT CCCAAGATAC ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.