p53 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	p53
Entrez Gene ID	2768677
Full Name	CG33336 gene product from transcript CG33336-RB
Synonyms	CG10873,CG31325,CG33336,D-p53,DMP53,Dm-P53,DmP53,Dmel\CG33336,Dmp53,Dp53,dmp53,dp53,prac
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94D10-94D10

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260323.2	NP_001247252.2	p53, isoform E
NM_206545.2	NP_996268.1	p53, isoform A
NM_206544.2	NP_996267.1	p53, isoform B
NM_001170223.1	NP_001163694.1	p53, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In mouse testes, p53 was required for heat-induced germ cell necrosis, indicating that regulation of necrosis is a primordial function of p53 conserved from invertebrates to vertebrates. Drosophila and mouse spermatogenesis will thus be useful models to identify inducers of necrosis to treat cancers that are refractory to apoptosis.
Title: p53-dependent programmed necrosis controls germ cell homeostasis during spermatogenesis.

SCE, together with dRYBP, inhibits apoptosis either by epigenetically regulating Dp53 transcription or by controlling the stabilization of DP53 protein levels thus promoting its ubiquitylation for proteaosomal degradation.
Title: Drosophila SCE/dRING E3-ligase inhibits apoptosis in a Dp53 dependent manner.

p53 deficiency induces cancer stem cell pool expansion in a mouse model of triple-negative breast tumors
Title: p53 deficiency induces cancer stem cell pool expansion in a mouse model of triple-negative breast tumors.

Third chromosome balancer inversions disrupt protein-coding genes, such as p53, and influence distal recombination events in Drosophila melanogaster.
Title: Third Chromosome Balancer Inversions Disrupt Protein-Coding Genes and Influence Distal Recombination Events in Drosophila melanogaster.

Dp53 is directly involved in the development of the cardiomyopathy induced by scox deficiency.
Title: Cardiac deficiency of single cytochrome oxidase assembly factor scox induces p53-dependent apoptosis in a Drosophila cardiomyopathy model.

The following p53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the p53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07265	NM_206544.2 Latest version!	Drosophila melanogaster p53, transcript variant B (p53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07266	NM_206545.2 Latest version!	Drosophila melanogaster p53, transcript variant A (p53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07266	NM_001170223.1 Latest version!	Drosophila melanogaster p53, transcript variant C (p53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa22349	NM_001260323.2 Latest version!	Drosophila melanogaster p53, transcript variant E (p53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

p53 ( NM_206544.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996267.1 Drosophila melanogaster p53, transcript variant B (p53), mRNA.

Price & Availability↑

CloneID	OFa07265
Clone ID Related Accession (Same CDS sequence)	NM_206544.2
Accession Version	NM_206544.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	p53, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_206544.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCTTC ACAAGTCCGC GTCGTTTAGC TTGACTTTTA ACCAAAACAC TTCGATTGTT 
TCGCGTAGCA ATAGTCGCAC AATTTTTGAA GCTTTCAAGG AGTTCCTGGA TTTTTGGGAT 
ATCGGCAACG AAGTTTCTGC AGAGTCAGCA GTTCGGGTCT CCAGCAACGG AGCTTTCAAC 
TTGCCGCAGA GTTTTGGCAA CGAATCCAAC GAATATGCCC ACCTGGCTAC GCCTGTGGAT 
CCAGCCTACG GAGGCAACAA CACGAACAAC ATGATGCAGT TCACGAACAA TCTGGAAATT 
TTGGCCAACA ATAATTCCGA TGGCAATAAC AAAATTAATG CATGCAACAA ATTCGTCTGC 
CACAAGGGCA CTGATTCCGA GGATGACTCC ACGGAGGTCG ATATCAAGGA GGATATTCCG 
AAAACGGTGG AGGTATCGGG ATCGGAATTG ACCACGGAAC CCATGGCCTT CTTGCAGGGA 
TTAAACTCCG GGAATCTGAT GCAGTTCAGC CAGCAATCCG TGCTGCGCGA AATGATGCTG 
CAGGACATTC AGATCCAGGC GAACACGCTG CCCAAGCTAG AGAATCACAA CATCGGTGGT 
TATTGCTTCA GCATGGTTCT GGATGAGCCG CCCAAGTCTC TTTGGATGTA CTCGATTCCG 
CTGAACAAGC TCTACATCCG GATGAACAAG GCCTTCAACG TGGACGTTCA GTTCAAGTCT 
AAAATGCCCA TCCAACCACT TAATTTGCGT GTGTTCCTTT GCTTCTCCAA TGATGTGAGT 
GCTCCCGTGG TCCGCTGTCA AAATCACCTT AGCGTTGAGC CTTTGACGGC CAATAACGCA 
AAAATGCGCG AGAGCTTGCT GCGCAGCGAG AATCCCAACA GTGTATATTG TGGAAATGCT 
CAGGGCAAGG GAATTTCCGA GCGTTTTTCC GTTGTAGTCC CCCTGAACAT GAGCCGGTCT 
GTAACCCGCA GTGGGCTCAC GCGCCAGACC CTGGCCTTCA AGTTCGTCTG CCAAAACTCG 
TGTATCGGGC GAAAAGAAAC TTCCTTAGTC TTCTGCCTGG AGAAAGCATG CGGCGATATC 
GTGGGACAGC ATGTTATACA TGTTAAAATA TGTACGTGCC CCAAGCGGGA TCGCATCCAA 
GACGAACGCC AGCTCAATAG CAAGAAGCGC AAGTCCGTGC CGGAAGCCGC CGAAGAAGAT 
GAGCCGTCCA AGGTGCGTCG GTGCATTGCT ATAAAGACGG AGGACACGGA GAGCAATGAT 
AGCCGAGACT GCGACGACTC CGCCGCAGAG TGGAACGTGT CGCGGACACC GGATGGCGAT 
TACCGTCTGG CTATTACGTG CCCCAATAAG GAATGGCTGC TGCAGAGCAT CGAGGGCATG 
ATTAAGGAGG CGGCGGCTGA AGTCCTGCGC AATCCCAACC AAGAGAATCT ACGTCGCCAT 
GCCAACAAAT TGCTGAGCCT TAAGAAACGT GCCTACGAGC TGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996267.1
CDS	75..1562
Translation	MSLHKSASFSLTFNQNTSIVSRSNSRTIFEAFKEFLDFWDIGNEVSAESAVRVSSNGAFNLPQSFGNESNEYAHLATPVDPAYGGNNTNNMMQFTNNLEILANNNSDGNNKINACNKFVCHKGTDSEDDSTEVDIKEDIPKTVEVSGSELTTEPMAFLQGLNSGNLMQFSQQSVLREMMLQDIQIQANTLPKLENHNIGGYCFSMVLDEPPKSLWMYSIPLNKLYIRMNKAFNVDVQFKSKMPIQPLNLRVFLCFSNDVSAPVVRCQNHLSVEPLTANNAKMRESLLRSENPNSVYCGNAQGKGISERFSVVVPLNMSRSVTRSGLTRQTLAFKFVCQNSCIGRKETSLVFCLEKACGDIVGQHVIHVKICTCPKRDRIQDERQLNSKKRKSVPEAAEEDEPSKVRRCIAIKTEDTESNDSRDCDDSAAEWNVSRTPDGDYRLAITCPNKEWLLQSIEGMIKEAAAEVLRNPNQENLRRHANKLLSLKKRAYELP

Target ORF information:

RefSeq Version	NM_206544.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster p53, transcript variant B (p53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206544.2

ORF Insert Sequence:

ATGAGTCTTC ACAAGTCCGC GTCGTTTAGC TTGACTTTTA ACCAAAACAC TTCGATTGTT 
TCGCGTAGCA ATAGTCGCAC AATTTTTGAA GCTTTCAAGG AGTTCCTGGA TTTTTGGGAT 
ATCGGCAACG AAGTTTCTGC AGAGTCAGCA GTTCGGGTCT CCAGCAACGG AGCTTTCAAC 
TTGCCGCAGA GTTTTGGCAA CGAATCCAAC GAATATGCCC ACCTGGCTAC GCCTGTGGAT 
CCAGCCTACG GAGGCAACAA CACGAACAAC ATGATGCAGT TCACGAACAA TCTGGAAATT 
TTGGCCAACA ATAATTCCGA TGGCAATAAC AAAATTAATG CATGCAACAA ATTCGTCTGC 
CACAAGGGCA CTGATTCCGA GGATGACTCC ACGGAGGTCG ATATCAAGGA GGATATTCCG 
AAAACGGTGG AGGTATCGGG ATCGGAATTG ACCACGGAAC CCATGGCCTT CTTGCAGGGA 
TTAAACTCCG GGAATCTGAT GCAGTTCAGC CAGCAATCCG TGCTGCGCGA AATGATGCTG 
CAGGACATTC AGATCCAGGC GAACACGCTG CCCAAGCTAG AGAATCACAA CATCGGTGGT 
TATTGCTTCA GCATGGTTCT GGATGAGCCG CCCAAGTCTC TTTGGATGTA CTCGATTCCG 
CTGAACAAGC TCTACATCCG GATGAACAAG GCCTTCAACG TGGACGTTCA GTTCAAGTCT 
AAAATGCCCA TCCAACCACT TAATTTGCGT GTGTTCCTTT GCTTCTCCAA TGATGTGAGT 
GCTCCCGTGG TCCGCTGTCA AAATCACCTT AGCGTTGAGC CTTTGACGGC CAATAACGCA 
AAAATGCGCG AGAGCTTGCT GCGCAGCGAG AATCCCAACA GTGTATATTG TGGAAATGCT 
CAGGGCAAGG GAATTTCCGA GCGTTTTTCC GTTGTAGTCC CCCTGAACAT GAGCCGGTCT 
GTAACCCGCA GTGGGCTCAC GCGCCAGACC CTGGCCTTCA AGTTCGTCTG CCAAAACTCG 
TGTATCGGGC GAAAAGAAAC TTCCTTAGTC TTCTGCCTGG AGAAAGCATG CGGCGATATC 
GTGGGACAGC ATGTTATACA TGTTAAAATA TGTACGTGCC CCAAGCGGGA TCGCATCCAA 
GACGAACGCC AGCTCAATAG CAAGAAGCGC AAGTCCGTGC CGGAAGCCGC CGAAGAAGAT 
GAGCCGTCCA AGGTGCGTCG GTGCATTGCT ATAAAGACGG AGGACACGGA GAGCAATGAT 
AGCCGAGACT GCGACGACTC CGCCGCAGAG TGGAACGTGT CGCGGACACC GGATGGCGAT 
TACCGTCTGG CTATTACGTG CCCCAATAAG GAATGGCTGC TGCAGAGCAT CGAGGGCATG 
ATTAAGGAGG CGGCGGCTGA AGTCCTGCGC AATCCCAACC AAGAGAATCT ACGTCGCCAT 
GCCAACAAAT TGCTGAGCCT TAAGAAACGT GCCTACGAGC TGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

p53 ( NM_206545.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996268.1 Drosophila melanogaster p53, transcript variant A (p53), mRNA.

Price & Availability↑

CloneID	OFa07266
Clone ID Related Accession (Same CDS sequence)	NM_206545.2 , NM_001170223.1
Accession Version	NM_206545.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	p53, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_206545.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATATAT CACAGCCAAT GTCGTGGCAC AAAGAAAGCA CTGATTCCGA GGATGACTCC 
ACGGAGGTCG ATATCAAGGA GGATATTCCG AAAACGGTGG AGGTATCGGG ATCGGAATTG 
ACCACGGAAC CCATGGCCTT CTTGCAGGGA TTAAACTCCG GGAATCTGAT GCAGTTCAGC 
CAGCAATCCG TGCTGCGCGA AATGATGCTG CAGGACATTC AGATCCAGGC GAACACGCTG 
CCCAAGCTAG AGAATCACAA CATCGGTGGT TATTGCTTCA GCATGGTTCT GGATGAGCCG 
CCCAAGTCTC TTTGGATGTA CTCGATTCCG CTGAACAAGC TCTACATCCG GATGAACAAG 
GCCTTCAACG TGGACGTTCA GTTCAAGTCT AAAATGCCCA TCCAACCACT TAATTTGCGT 
GTGTTCCTTT GCTTCTCCAA TGATGTGAGT GCTCCCGTGG TCCGCTGTCA AAATCACCTT 
AGCGTTGAGC CTTTGACGGC CAATAACGCA AAAATGCGCG AGAGCTTGCT GCGCAGCGAG 
AATCCCAACA GTGTATATTG TGGAAATGCT CAGGGCAAGG GAATTTCCGA GCGTTTTTCC 
GTTGTAGTCC CCCTGAACAT GAGCCGGTCT GTAACCCGCA GTGGGCTCAC GCGCCAGACC 
CTGGCCTTCA AGTTCGTCTG CCAAAACTCG TGTATCGGGC GAAAAGAAAC TTCCTTAGTC 
TTCTGCCTGG AGAAAGCATG CGGCGATATC GTGGGACAGC ATGTTATACA TGTTAAAATA 
TGTACGTGCC CCAAGCGGGA TCGCATCCAA GACGAACGCC AGCTCAATAG CAAGAAGCGC 
AAGTCCGTGC CGGAAGCCGC CGAAGAAGAT GAGCCGTCCA AGGTGCGTCG GTGCATTGCT 
ATAAAGACGG AGGACACGGA GAGCAATGAT AGCCGAGACT GCGACGACTC CGCCGCAGAG 
TGGAACGTGT CGCGGACACC GGATGGCGAT TACCGTCTGG CTATTACGTG CCCCAATAAG 
GAATGGCTGC TGCAGAGCAT CGAGGGCATG ATTAAGGAGG CGGCGGCTGA AGTCCTGCGC 
AATCCCAACC AAGAGAATCT ACGTCGCCAT GCCAACAAAT TGCTGAGCCT TAAGAAACGT 
GCCTACGAGC TGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996268.1
CDS	112..1269
Translation	MYISQPMSWHKESTDSEDDSTEVDIKEDIPKTVEVSGSELTTEPMAFLQGLNSGNLMQFSQQSVLREMMLQDIQIQANTLPKLENHNIGGYCFSMVLDEPPKSLWMYSIPLNKLYIRMNKAFNVDVQFKSKMPIQPLNLRVFLCFSNDVSAPVVRCQNHLSVEPLTANNAKMRESLLRSENPNSVYCGNAQGKGISERFSVVVPLNMSRSVTRSGLTRQTLAFKFVCQNSCIGRKETSLVFCLEKACGDIVGQHVIHVKICTCPKRDRIQDERQLNSKKRKSVPEAAEEDEPSKVRRCIAIKTEDTESNDSRDCDDSAAEWNVSRTPDGDYRLAITCPNKEWLLQSIEGMIKEAAAEVLRNPNQENLRRHANKLLSLKKRAYELP

Target ORF information:

RefSeq Version	NM_206545.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster p53, transcript variant A (p53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206545.2

ORF Insert Sequence:

ATGTATATAT CACAGCCAAT GTCGTGGCAC AAAGAAAGCA CTGATTCCGA GGATGACTCC 
ACGGAGGTCG ATATCAAGGA GGATATTCCG AAAACGGTGG AGGTATCGGG ATCGGAATTG 
ACCACGGAAC CCATGGCCTT CTTGCAGGGA TTAAACTCCG GGAATCTGAT GCAGTTCAGC 
CAGCAATCCG TGCTGCGCGA AATGATGCTG CAGGACATTC AGATCCAGGC GAACACGCTG 
CCCAAGCTAG AGAATCACAA CATCGGTGGT TATTGCTTCA GCATGGTTCT GGATGAGCCG 
CCCAAGTCTC TTTGGATGTA CTCGATTCCG CTGAACAAGC TCTACATCCG GATGAACAAG 
GCCTTCAACG TGGACGTTCA GTTCAAGTCT AAAATGCCCA TCCAACCACT TAATTTGCGT 
GTGTTCCTTT GCTTCTCCAA TGATGTGAGT GCTCCCGTGG TCCGCTGTCA AAATCACCTT 
AGCGTTGAGC CTTTGACGGC CAATAACGCA AAAATGCGCG AGAGCTTGCT GCGCAGCGAG 
AATCCCAACA GTGTATATTG TGGAAATGCT CAGGGCAAGG GAATTTCCGA GCGTTTTTCC 
GTTGTAGTCC CCCTGAACAT GAGCCGGTCT GTAACCCGCA GTGGGCTCAC GCGCCAGACC 
CTGGCCTTCA AGTTCGTCTG CCAAAACTCG TGTATCGGGC GAAAAGAAAC TTCCTTAGTC 
TTCTGCCTGG AGAAAGCATG CGGCGATATC GTGGGACAGC ATGTTATACA TGTTAAAATA 
TGTACGTGCC CCAAGCGGGA TCGCATCCAA GACGAACGCC AGCTCAATAG CAAGAAGCGC 
AAGTCCGTGC CGGAAGCCGC CGAAGAAGAT GAGCCGTCCA AGGTGCGTCG GTGCATTGCT 
ATAAAGACGG AGGACACGGA GAGCAATGAT AGCCGAGACT GCGACGACTC CGCCGCAGAG 
TGGAACGTGT CGCGGACACC GGATGGCGAT TACCGTCTGG CTATTACGTG CCCCAATAAG 
GAATGGCTGC TGCAGAGCAT CGAGGGCATG ATTAAGGAGG CGGCGGCTGA AGTCCTGCGC 
AATCCCAACC AAGAGAATCT ACGTCGCCAT GCCAACAAAT TGCTGAGCCT TAAGAAACGT 
GCCTACGAGC TGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

p53 ( NM_001170223.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163694.1 Drosophila melanogaster p53, transcript variant C (p53), mRNA.

Price & Availability↑

CloneID	OFa07266
Clone ID Related Accession (Same CDS sequence)	NM_206545.2 , NM_001170223.1
Accession Version	NM_001170223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	p53, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATATAT CACAGCCAAT GTCGTGGCAC AAAGAAAGCA CTGATTCCGA GGATGACTCC 
ACGGAGGTCG ATATCAAGGA GGATATTCCG AAAACGGTGG AGGTATCGGG ATCGGAATTG 
ACCACGGAAC CCATGGCCTT CTTGCAGGGA TTAAACTCCG GGAATCTGAT GCAGTTCAGC 
CAGCAATCCG TGCTGCGCGA AATGATGCTG CAGGACATTC AGATCCAGGC GAACACGCTG 
CCCAAGCTAG AGAATCACAA CATCGGTGGT TATTGCTTCA GCATGGTTCT GGATGAGCCG 
CCCAAGTCTC TTTGGATGTA CTCGATTCCG CTGAACAAGC TCTACATCCG GATGAACAAG 
GCCTTCAACG TGGACGTTCA GTTCAAGTCT AAAATGCCCA TCCAACCACT TAATTTGCGT 
GTGTTCCTTT GCTTCTCCAA TGATGTGAGT GCTCCCGTGG TCCGCTGTCA AAATCACCTT 
AGCGTTGAGC CTTTGACGGC CAATAACGCA AAAATGCGCG AGAGCTTGCT GCGCAGCGAG 
AATCCCAACA GTGTATATTG TGGAAATGCT CAGGGCAAGG GAATTTCCGA GCGTTTTTCC 
GTTGTAGTCC CCCTGAACAT GAGCCGGTCT GTAACCCGCA GTGGGCTCAC GCGCCAGACC 
CTGGCCTTCA AGTTCGTCTG CCAAAACTCG TGTATCGGGC GAAAAGAAAC TTCCTTAGTC 
TTCTGCCTGG AGAAAGCATG CGGCGATATC GTGGGACAGC ATGTTATACA TGTTAAAATA 
TGTACGTGCC CCAAGCGGGA TCGCATCCAA GACGAACGCC AGCTCAATAG CAAGAAGCGC 
AAGTCCGTGC CGGAAGCCGC CGAAGAAGAT GAGCCGTCCA AGGTGCGTCG GTGCATTGCT 
ATAAAGACGG AGGACACGGA GAGCAATGAT AGCCGAGACT GCGACGACTC CGCCGCAGAG 
TGGAACGTGT CGCGGACACC GGATGGCGAT TACCGTCTGG CTATTACGTG CCCCAATAAG 
GAATGGCTGC TGCAGAGCAT CGAGGGCATG ATTAAGGAGG CGGCGGCTGA AGTCCTGCGC 
AATCCCAACC AAGAGAATCT ACGTCGCCAT GCCAACAAAT TGCTGAGCCT TAAGAAACGT 
GCCTACGAGC TGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163694.1
CDS	630..1787
Translation	MYISQPMSWHKESTDSEDDSTEVDIKEDIPKTVEVSGSELTTEPMAFLQGLNSGNLMQFSQQSVLREMMLQDIQIQANTLPKLENHNIGGYCFSMVLDEPPKSLWMYSIPLNKLYIRMNKAFNVDVQFKSKMPIQPLNLRVFLCFSNDVSAPVVRCQNHLSVEPLTANNAKMRESLLRSENPNSVYCGNAQGKGISERFSVVVPLNMSRSVTRSGLTRQTLAFKFVCQNSCIGRKETSLVFCLEKACGDIVGQHVIHVKICTCPKRDRIQDERQLNSKKRKSVPEAAEEDEPSKVRRCIAIKTEDTESNDSRDCDDSAAEWNVSRTPDGDYRLAITCPNKEWLLQSIEGMIKEAAAEVLRNPNQENLRRHANKLLSLKKRAYELP

Target ORF information:

RefSeq Version	NM_001170223.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster p53, transcript variant C (p53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170223.1

ORF Insert Sequence:

ATGTATATAT CACAGCCAAT GTCGTGGCAC AAAGAAAGCA CTGATTCCGA GGATGACTCC 
ACGGAGGTCG ATATCAAGGA GGATATTCCG AAAACGGTGG AGGTATCGGG ATCGGAATTG 
ACCACGGAAC CCATGGCCTT CTTGCAGGGA TTAAACTCCG GGAATCTGAT GCAGTTCAGC 
CAGCAATCCG TGCTGCGCGA AATGATGCTG CAGGACATTC AGATCCAGGC GAACACGCTG 
CCCAAGCTAG AGAATCACAA CATCGGTGGT TATTGCTTCA GCATGGTTCT GGATGAGCCG 
CCCAAGTCTC TTTGGATGTA CTCGATTCCG CTGAACAAGC TCTACATCCG GATGAACAAG 
GCCTTCAACG TGGACGTTCA GTTCAAGTCT AAAATGCCCA TCCAACCACT TAATTTGCGT 
GTGTTCCTTT GCTTCTCCAA TGATGTGAGT GCTCCCGTGG TCCGCTGTCA AAATCACCTT 
AGCGTTGAGC CTTTGACGGC CAATAACGCA AAAATGCGCG AGAGCTTGCT GCGCAGCGAG 
AATCCCAACA GTGTATATTG TGGAAATGCT CAGGGCAAGG GAATTTCCGA GCGTTTTTCC 
GTTGTAGTCC CCCTGAACAT GAGCCGGTCT GTAACCCGCA GTGGGCTCAC GCGCCAGACC 
CTGGCCTTCA AGTTCGTCTG CCAAAACTCG TGTATCGGGC GAAAAGAAAC TTCCTTAGTC 
TTCTGCCTGG AGAAAGCATG CGGCGATATC GTGGGACAGC ATGTTATACA TGTTAAAATA 
TGTACGTGCC CCAAGCGGGA TCGCATCCAA GACGAACGCC AGCTCAATAG CAAGAAGCGC 
AAGTCCGTGC CGGAAGCCGC CGAAGAAGAT GAGCCGTCCA AGGTGCGTCG GTGCATTGCT 
ATAAAGACGG AGGACACGGA GAGCAATGAT AGCCGAGACT GCGACGACTC CGCCGCAGAG 
TGGAACGTGT CGCGGACACC GGATGGCGAT TACCGTCTGG CTATTACGTG CCCCAATAAG 
GAATGGCTGC TGCAGAGCAT CGAGGGCATG ATTAAGGAGG CGGCGGCTGA AGTCCTGCGC 
AATCCCAACC AAGAGAATCT ACGTCGCCAT GCCAACAAAT TGCTGAGCCT TAAGAAACGT 
GCCTACGAGC TGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

p53 ( NM_001260323.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247252.2 Drosophila melanogaster p53, transcript variant E (p53), mRNA.

Price & Availability↑

CloneID	OFa22349
Clone ID Related Accession (Same CDS sequence)	NM_001260323.2
Accession Version	NM_001260323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	p53, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260323.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCTCT ATCAGCTCTA TGAGCGCAAA CAATCCGTGC TGCGCGAAAT GATGCTGCAG 
GACATTCAGA TCCAGGCGAA CACGCTGCCC AAGCTAGAGA ATCACAACAT CGGTGGTTAT 
TGCTTCAGCA TGGTTCTGGA TGAGCCGCCC AAGTCTCTTT GGATGTACTC GATTCCGCTG 
AACAAGCTCT ACATCCGGAT GAACAAGGCC TTCAACGTGG ACGTTCAGTT CAAGTCTAAA 
ATGCCCATCC AACCACTTAA TTTGCGTGTG TTCCTTTGCT TCTCCAATGA TGTGAGTGCT 
CCCGTGGTCC GCTGTCAAAA TCACCTTAGC GTTGAGCCTT TGACGGCCAA TAACGCAAAA 
ATGCGCGAGA GCTTGCTGCG CAGCGAGAAT CCCAACAGTG TATATTGTGG AAATGCTCAG 
GGCAAGGGAA TTTCCGAGCG TTTTTCCGTT GTAGTCCCCC TGAACATGAG CCGGTCTGTA 
ACCCGCAGTG GGCTCACGCG CCAGACCCTG GCCTTCAAGT TCGTCTGCCA AAACTCGTGT 
ATCGGGCGAA AAGAAACTTC CTTAGTCTTC TGCCTGGAGA AAGCATGCGG CGATATCGTG 
GGACAGCATG TTATACATGT TAAAATATGT ACGTGCCCCA AGCGGGATCG CATCCAAGAC 
GAACGCCAGC TCAATAGCAA GAAGCGCAAG TCCGTGCCGG AAGCCGCCGA AGAAGATGAG 
CCGTCCAAGG TGCGTCGGTG CATTGCTATA AAGACGGAGG ACACGGAGAG CAATGATAGC 
CGAGACTGCG ACGACTCCGC CGCAGAGTGG AACGTGTCGC GGACACCGGA TGGCGATTAC 
CGTCTGGCTA TTACGTGCCC CAATAAGGAA TGGCTGCTGC AGAGCATCGA GGGCATGATT 
AAGGAGGCGG CGGCTGAAGT CCTGCGCAAT CCCAACCAAG AGAATCTACG TCGCCATGCC 
AACAAATTGC TGAGCCTTAA GAAACGTGCC TACGAGCTGC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247252.2
CDS	12..1016
Translation	MKLYQLYERKQSVLREMMLQDIQIQANTLPKLENHNIGGYCFSMVLDEPPKSLWMYSIPLNKLYIRMNKAFNVDVQFKSKMPIQPLNLRVFLCFSNDVSAPVVRCQNHLSVEPLTANNAKMRESLLRSENPNSVYCGNAQGKGISERFSVVVPLNMSRSVTRSGLTRQTLAFKFVCQNSCIGRKETSLVFCLEKACGDIVGQHVIHVKICTCPKRDRIQDERQLNSKKRKSVPEAAEEDEPSKVRRCIAIKTEDTESNDSRDCDDSAAEWNVSRTPDGDYRLAITCPNKEWLLQSIEGMIKEAAAEVLRNPNQENLRRHANKLLSLKKRAYELP

Target ORF information:

RefSeq Version	NM_001260323.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster p53, transcript variant E (p53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260323.2

ORF Insert Sequence:

ATGAAGCTCT ATCAGCTCTA TGAGCGCAAA CAATCCGTGC TGCGCGAAAT GATGCTGCAG 
GACATTCAGA TCCAGGCGAA CACGCTGCCC AAGCTAGAGA ATCACAACAT CGGTGGTTAT 
TGCTTCAGCA TGGTTCTGGA TGAGCCGCCC AAGTCTCTTT GGATGTACTC GATTCCGCTG 
AACAAGCTCT ACATCCGGAT GAACAAGGCC TTCAACGTGG ACGTTCAGTT CAAGTCTAAA 
ATGCCCATCC AACCACTTAA TTTGCGTGTG TTCCTTTGCT TCTCCAATGA TGTGAGTGCT 
CCCGTGGTCC GCTGTCAAAA TCACCTTAGC GTTGAGCCTT TGACGGCCAA TAACGCAAAA 
ATGCGCGAGA GCTTGCTGCG CAGCGAGAAT CCCAACAGTG TATATTGTGG AAATGCTCAG 
GGCAAGGGAA TTTCCGAGCG TTTTTCCGTT GTAGTCCCCC TGAACATGAG CCGGTCTGTA 
ACCCGCAGTG GGCTCACGCG CCAGACCCTG GCCTTCAAGT TCGTCTGCCA AAACTCGTGT 
ATCGGGCGAA AAGAAACTTC CTTAGTCTTC TGCCTGGAGA AAGCATGCGG CGATATCGTG 
GGACAGCATG TTATACATGT TAAAATATGT ACGTGCCCCA AGCGGGATCG CATCCAAGAC 
GAACGCCAGC TCAATAGCAA GAAGCGCAAG TCCGTGCCGG AAGCCGCCGA AGAAGATGAG 
CCGTCCAAGG TGCGTCGGTG CATTGCTATA AAGACGGAGG ACACGGAGAG CAATGATAGC 
CGAGACTGCG ACGACTCCGC CGCAGAGTGG AACGTGTCGC GGACACCGGA TGGCGATTAC 
CGTCTGGCTA TTACGTGCCC CAATAAGGAA TGGCTGCTGC AGAGCATCGA GGGCATGATT 
AAGGAGGCGG CGGCTGAAGT CCTGCGCAAT CCCAACCAAG AGAATCTACG TCGCCATGCC 
AACAAATTGC TGAGCCTTAA GAAACGTGCC TACGAGCTGC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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p53 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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