Tmc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmc
Entrez Gene ID	26067066
Full Name	Transmembrane channel-like
Synonyms	CG14177,CG3280,CG46121,Dmel\CG46121,Dmel_CG14177,Dmel_CG3280,tmc
General protein information	Preferred Names Transmembrane channel-like Names CG46121 gene product from transcript CG46121-RC CG46121-PC CG46121-PD Tmc-PC Tmc-PD transmembrane channel-like
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67C2-67C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001316433.1	NP_001303362.1	transmembrane channel-like, isoform C
NM_001259769.3	NP_001246698.1	transmembrane channel-like, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the single Drosophila transmembrane channel- like (TMC) protein is expressed in md-L neurons, where it is required for sensing two key textural features of food-hardness and viscosity.
Title: The Basis of Food Texture Sensation in Drosophila.

Drosophila melanogaster transmembrane channel-like (tmc) gene is expressed in the larval class I and class II dendritic arborization (da) neurons and bipolar dendrite (bd) neurons, both of which are known to provide sensory feedback for larval locomotion
Title: Transmembrane channel-like (tmc) gene regulates Drosophila larval locomotion.

The following Tmc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20792	NM_001259769.3 Latest version!	Drosophila melanogaster transmembrane channel-like, transcript variant D (Tmc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa41364	NM_001316433.1 Latest version!	Drosophila melanogaster transmembrane channel-like, transcript variant C (Tmc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmc ( NM_001259769.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246698.1 Drosophila melanogaster transmembrane channel-like, transcript variant D (Tmc), mRNA.

Price & Availability↑

CloneID	OFa20792
Clone ID Related Accession (Same CDS sequence)	NM_001259769.3
Accession Version	NM_001259769.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transmembrane channel-like, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Oct 19, 2015 this sequence version replaced NM_001259769.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAGCA GCGCCGATGC CAAGAGGGAA TTCTACAAAG GACAGCGAGG CAGGAGAGCT 
GCGTCACAGG AGGACCACAG ATCCTACGAA CTGAACGATT TCCCATTGCA AAACCAGAGC 
AGCGACGCCG AGAGTTGCCA CCAGGAGCCT CACTTTGCCC ACCAGCGTTC GCCTGGAATC 
GGATTCGACG AGGATGGCGG CGGTGGCGAC ATTGACGATG AGGAGAGCTA CACAATTTCC 
GTATCGGCAA TAATGCAGAG ACGTGCCAGT GTGCGCGGCT ATCGGGGCAA ACGCGGCAGC 
CGGAGTTCGC GACGTGCATC CAGTCCCATG GATCACGTTC TGGACAGTGT GGAGCGACGA 
CGCTCCAGTG TCTATACGAC AAGTTCAGAG GAGGGCACCA ATCAGGAGTC AACGCAAGAG 
CAAATCTTTG AGAACATCCG CCTGCACAAG GAGGTCATAC AGTCGGTGAA GCTACAGCCA 
TGGCCCATCC GCAAGAAGCT CAAGTTGGTG CGGCAGGCCA AAACATATGT GGCACGCCAC 
GAGGGCGCGC TCCAGGAGCG CTTCGCCATG TCACGCAGCA CCAGAGACTT GTGGGCGAGG 
TTTAAAATTT TAATGGCGGC ACGATGGCGA CACTGGAAAC GTGAGACGGC CAGCTTCCTT 
ACCGTCCTCA TTCCCTGGGA GCTGCGCATC AAGGAAATCG AATCACACTT CGGCTCCGGC 
GTAGCCTCCT ACTTTACATT CCTGCGATGG CTTATGTGGG TCAATATCAT GATTGCCATT 
CCGCTGGTCG CCTTCGTCAT TGGCCCCGAG TACTTTGCCA CAAAGCACGG CGAAACGGAT 
CCCAGGAAAA GGATGTCCGA CCCGGAGGCT CGGGTGGCCG GCAATCTTTT CACGTTCTGG 
GAGTTCGAGG GATACCTGAA ATATTCACCT ATGTTTTATG GATACTATTC AAGCACATCC 
GGCATTAGCA CGTCCGGATA TAAGCTTCCC TTAGCCTATT TCCTTACCGC CGTTCTGGTC 
TACATCTACA GCTTTGTGGC CACGTTGAGG AAAATGGCGG AAAACTCGCG CAACTCAAAA 
CTTTCCTCGA AGGACGACGA GTGCGTCTTC TCGTGGAAGC TCTTCACCGG CTGGGACTTT 
ATGATTGGAC ACGCGGAAAC GGCGCACAAT CGCATCGCAT CCGTGGTCGT GGGCTTCAAG 
GAGGCACTGC TGGAGGAAGC GGAGAAAAAG AAGGATAATC GCAACTGGCG GGTCATACTC 
CAGAGGATAC TGGTCAATAT ACTGGTGATG GGGCTGCTGG GCTTGTCGGG CGCTACGGTC 
GTGCTGCTGG TTAATCACTC TGAGGATTTG GCCAAGCATG ACAACTGGCT GAGTCGCAAT 
GCGGTGAACG TGACCATGAC CCTGCTCTCC TTCTTCCTGC CCATGATATT CGAGGCACTG 
GGTCTGTTCG AGAACTGGCA TCCCAGGCAG CAGCTGCGTT TGCAATTGGC TCGCATTATG 
ATCCTGAACA TGTTAAACTT GTACTCGCTG ATGTTCTCCT TCATCTACAA GATCAACAGC 
AAGGAGAAAC CGCTACAGAT GTTGAAATTG GAGAACGAAA CAAACACCAT GGAGCTAAAG 
AATCTACTTA GTTCAATTGA GGCTCTGAGG GCCATGACAC CCACGACATC GCTATATGGT 
GAAAGTACTT CGGATGGGCT ATTCGATGAC TCTACATCAA CGGCAACTTG GGGTGAGGAT 
GGTGGTGGCC TCTTTTCGAC CACAGCGGCT GCTGCCTTAA TCTCCACCAC CGTGCAGCGT 
CTCAAGTGTT ACAACATGAC TGTAAAATGC TCAAAGCTGC GGCGCAACAT CATCAGTGGC 
AAGCACTTGG CCACCACTCT GATGGTTCTC AACCTGACAA CACCAGCCAT GGTGCCACCA 
ACTTTGCCAA CCACATTGCC CACCACATTT CCCACCACAT TGCCCACCAC ATTACCCACT 
ACATTACCTA CAGCATTGCC TACCACATTG CCCACCACAT TGCCTACTAC ATTGCCCTCC 
ACATTAGCCA CCACAACGGC GACGACTTCA AGTATTTGGT CAACCACAGA GGAAACATCT 
CCGACAACTA CAACGACATC ACCTTGGACC ACGTTACCGC CCTCGACAAC AACCACTGAG 
GCAACCACAA CGACTGAAAG AGCCACCACA ACCACAGAAG CAACGAGCAC AACCACACTG 
AAGATCACCA CTGCCGAAAT AAATAGTACA CTATCAGATA CTACAAAACC CCTGGGAAAA 
TCTATTGATA CTGAAATACC CAACTCAACA ACGAATTCTG CCACTTTGAG CACAATACCA 
GCCACTTTAA ACACCACTAA TTTACCCCTG AACAGCACCA CCAAACTAAC TACAACCACA 
TCCACGGAGA AGCCCCAGGG AGAGGATAAC TTTATCTACA CCACCGGAGA AGATGAGGGC 
TCCTACGATT ATGGCAGTGA TTCCACATCA GACGCTCCGG ATAATAATAG CTACTCCGAT 
ATAACGGATT ACTCTTCGGA ACCGTCGGAA ATTGAAGATT TCGATGAACA GGAAAGCACG 
GATCAGGCGG ACGATCCACT GGCCAAAGTA TTGGAGCAGC TCGATGAAAA TGAGACGAAG 
GGACGCAGGA AAAGGGCATT GGCCGAATCA CCATTCTTTA CCAGCAAGTA CAGCAGGCGA 
CATCGCAATG AATCCGCTGT CTCAGCTGGA CAACCACGAG AAACCACTGA ATCGGTGAAT 
GCCACACCCA GTCGGTGGCC ATTTAATTGG GCATCTTTTA GGCAAACCAC ACCGAGAACC 
ACCACAACAA GAAGAGTTCC CAGTGGAATT CTAACCAAGG AGGAATGGGA AAGACTCAGA 
CGACTTAGGG GCAGGATTAC CACAACTACC AGTACGTCCA CCACCAGCAC AACCACAAGA 
AGACCCAGGT GGAGATATCG AACCACGACT ACCGAACTTA CCAGCACCAC CGAGGAAGAA 
TCCTCCACAA CGGAGAGTTC CACTGATTCA TCGAGTCCAG GGAGCACTAC CAATGCCTTC 
GACAGCAGCA GTAGCACCAC CGAAGAAGAT GAATACACCA CCACGGAGGG ATCGGAGAAT 
AGACCCTACT ATGTGGGCTA CGTGGACATA TCGGAAATGG GAAGTACTAT CTACTACGAT 
GGAGATAGTG AATTTCTGGA GGAGTGTGTG ATTACCATTT GCCCCAAGGG CGATGACTTT 
TTTGGCAGCA CCACTGAAAG TCCGGATAGC ACCACCCAGA GCAGCGACTC CAAGCAGCTA 
ACCACAGTGA AGCTCACTCC GCTGGAGCGG AAGCAGAAGC GTTTGAAGGA AGTGCAGCTG 
GCGATTAAGC AAATACAAAC CAATCTGACG ACCATGTGCT GGGAGACCTC CTTGGGCCAG 
GAGCTCTCCA AAGTCATCGT TTTCGATGGG CTGATGTCCA TTGTGGCGCC GCTGTGCATC 
GACTTTCTGC GCGCCCTCTT CGTCCGGTAT GTCAATCAAA ACTGGTGCTG GGACATGGAG 
AAGACCTTTC CCCAGTACGG TGACTTCAAG ATAGCCGAGA ACATCCTGAC GTTGATTAAT 
AACCAGGGCC AAGTGTGGAT GGGCATATTC TTCTCGCCGG GCCTGGTGCT TATCAACCTG 
GTCAAATTGA TGATCATGAT GTACTTCCGT TCGTGGATAG TGCTCACCTG CAATGTGCCG 
CATGAGGTGG TGTTCAAGGC CTCCAAGTCG AACAACTTCT ACCTATCGCT GCTGTTGACC 
ATGCTGTTCC TTTGTGTCCT GCCCGTGGGC TATGCGATTG TGTGGCTGCG TCCTTCCTGG 
CATTGTGGAC CCTTCTCGGA GTACAACAGG ATAGCTGAGT TCATTACCAA CACCACGAGG 
AATGCTCTGC CAAAACAACT CCACGAACCC CTGGACTATC TGACCTCCTC GAGCACTGTG 
ATTCCATTGC TTCTGCTTCT TATCCTTATT ATATACTACC TGGTTTCGCT AACTGGCGCT 
CTACGCGAAG CCAACCAGGA TTTGCGCACC CAACTCCAGA AGGAACGCGA GGAGGAGCGC 
AAGAAGATCT TTAAGGTGCC GGAGGTCAAA CAAGCGGAAC CAACGGCCAC CACGCTGACA 
AATCGCTGGC GCAAAGTACT CGAGGCCAGC TCTCCGGTGA CACCCACCCA ACCACCCGAT 
TTCGATACGG AGGAGTACAA GAATCAAGCC CGGAAAGAGC TAATCTCACG CATCATGAAG 
AAGGCGTTGC GCAAGGGATC CGCCACGTCG GATGAGGATT CGTTTGTGCG GCGCGACGAC 
GATGACACGG ACACCGAGCA TCAGGACTCC CTGCCCCACG ACGAGGAGGC CAAGGACAAG 
CGGTTCGGCC TGTCGCGATT GCAGCAAATC CGGCGTACCC GCAAACCATC GCTGGTGGAC 
ATCGTTCAGA TTGCCAAGCA GGAGCGGGCA CGGGCGGGAT CCATTGTGGC CGGAACAAGC 
TCCTCTGGAA CTGGCAATTT TCCCATAAAG GAGACCCATC CAAAGAGCCG CTTCAAAGTG 
GAGAAACATG AGCGCAAGGA TAGGGGCAGC ATGAAGGATA AAAAGGACAC CAGGCACAGA 
CAGTCGCCAC AGCAGCAGCA ACAGCCGCCG CCCTATGAAT CGCCCAAGGA CAATGAACAT 
GATCCGGATA CAAATTCAAG AATTGTCAGC GCCAGTTTGC TGAGGCGCCA CAAGGAGCAG 
GCGGAGGGAG AGGAACCACC CACTACCCCC GACGCACCAC AAACACCCAA CTCGCCAGTA 
GAGCCCGTGG AACAGGCGCT CGAGGAATCC ACACCCGAAA CTCCCACGCT GGCCAAAAGC 
AAATTCCACA TAGTTGATGA AAAAAAACCC CCGCCGCATG AAGTGGAAGA TAAACCCTTG 
CCGACACCCA AGGAGAGTGG CAGTGGCGGT GGAAGTTTGG GCAAGTTCAA GTTCCGTAAG 
CACAAATTCA AGTCAAATAA TGTGGCTGCC GTCAAGCCGG AGCCAGAGGT GTTCAAGTTT 
GACGAGCGGA GTGTGGAAAG GAGTTCGGAT GTACCGGCAA CTCACGCCGC AGAGTACCTA 
AACAACGAGC CATCCGGCAC TGAGGAGCAG GATAGGAGTC TGCCATCACC CACTCCCAGC 
CAGGGTCAGG GTCACCACCA GCGGCAATTG TCTGTGCTTT CCCGGCAGGG TCGCAAGAAG 
ATCGGCAACC TGTTGGCCCT GGTTCGTGAA GCCGTCAATC TCAAAAAGGA CGATGTGGAG 
CAGGCGGGCT CGGATGAGTC GCCGGGTCCC ACAACGCCCA CCTACTTGGC CTACACACCG 
CCACCACCAC CATCAGTATT GTCCAGCGTG TCCAGTTCGA CGGCCTTGGA AATGCCACCT 
ACTCCGGAAC CGGAATCTCC AACGCCCAGT GCACCACTGC ACTTTGGCAG CAGCACATCG 
TCTCGTGCGC CTTCCAAGCC ACCAAAGCCT CCAATGGTTC CCGCATCCGC CACTGCTCCA 
ACGGCCACCA TGGATGACCT GGAGGAACTG GACACAGCCG GTCCTATTAC ATTTCCCAGG 
CGCAGCGATT CCCATCGCCG TCGCACTATG AGACAGGATT CACAGAGCTC CATTTGGTCG 
GATAATATAC CCACTATTAC TATAAGCACC ACCGGCAGCG ATGAGTGCAT TGTGGACGCA 
GCTGCTCCAC AAAATGGGCT TCCCGACCCG CGGAGTGCTT CCCCGGAGCC CACGGTCAAC 
ATTATAAGGA TTGACATTGA AAATGAGCAT GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246698.1
CDS	354..6152
Translation	MQSSADAKREFYKGQRGRRAASQEDHRSYELNDFPLQNQSSDAESCHQEPHFAHQRSPGIGFDEDGGGGDIDDEESYTISVSAIMQRRASVRGYRGKRGSRSSRRASSPMDHVLDSVERRRSSVYTTSSEEGTNQESTQEQIFENIRLHKEVIQSVKLQPWPIRKKLKLVRQAKTYVARHEGALQERFAMSRSTRDLWARFKILMAARWRHWKRETASFLTVLIPWELRIKEIESHFGSGVASYFTFLRWLMWVNIMIAIPLVAFVIGPEYFATKHGETDPRKRMSDPEARVAGNLFTFWEFEGYLKYSPMFYGYYSSTSGISTSGYKLPLAYFLTAVLVYIYSFVATLRKMAENSRNSKLSSKDDECVFSWKLFTGWDFMIGHAETAHNRIASVVVGFKEALLEEAEKKKDNRNWRVILQRILVNILVMGLLGLSGATVVLLVNHSEDLAKHDNWLSRNAVNVTMTLLSFFLPMIFEALGLFENWHPRQQLRLQLARIMILNMLNLYSLMFSFIYKINSKEKPLQMLKLENETNTMELKNLLSSIEALRAMTPTTSLYGESTSDGLFDDSTSTATWGEDGGGLFSTTAAAALISTTVQRLKCYNMTVKCSKLRRNIISGKHLATTLMVLNLTTPAMVPPTLPTTLPTTFPTTLPTTLPTTLPTALPTTLPTTLPTTLPSTLATTTATTSSIWSTTEETSPTTTTTSPWTTLPPSTTTTEATTTTERATTTTEATSTTTLKITTAEINSTLSDTTKPLGKSIDTEIPNSTTNSATLSTIPATLNTTNLPLNSTTKLTTTTSTEKPQGEDNFIYTTGEDEGSYDYGSDSTSDAPDNNSYSDITDYSSEPSEIEDFDEQESTDQADDPLAKVLEQLDENETKGRRKRALAESPFFTSKYSRRHRNESAVSAGQPRETTESVNATPSRWPFNWASFRQTTPRTTTTRRVPSGILTKEEWERLRRLRGRITTTTSTSTTSTTTRRPRWRYRTTTTELTSTTEEESSTTESSTDSSSPGSTTNAFDSSSSTTEEDEYTTTEGSENRPYYVGYVDISEMGSTIYYDGDSEFLEECVITICPKGDDFFGSTTESPDSTTQSSDSKQLTTVKLTPLERKQKRLKEVQLAIKQIQTNLTTMCWETSLGQELSKVIVFDGLMSIVAPLCIDFLRALFVRYVNQNWCWDMEKTFPQYGDFKIAENILTLINNQGQVWMGIFFSPGLVLINLVKLMIMMYFRSWIVLTCNVPHEVVFKASKSNNFYLSLLLTMLFLCVLPVGYAIVWLRPSWHCGPFSEYNRIAEFITNTTRNALPKQLHEPLDYLTSSSTVIPLLLLLILIIYYLVSLTGALREANQDLRTQLQKEREEERKKIFKVPEVKQAEPTATTLTNRWRKVLEASSPVTPTQPPDFDTEEYKNQARKELISRIMKKALRKGSATSDEDSFVRRDDDDTDTEHQDSLPHDEEAKDKRFGLSRLQQIRRTRKPSLVDIVQIAKQERARAGSIVAGTSSSGTGNFPIKETHPKSRFKVEKHERKDRGSMKDKKDTRHRQSPQQQQQPPPYESPKDNEHDPDTNSRIVSASLLRRHKEQAEGEEPPTTPDAPQTPNSPVEPVEQALEESTPETPTLAKSKFHIVDEKKPPPHEVEDKPLPTPKESGSGGGSLGKFKFRKHKFKSNNVAAVKPEPEVFKFDERSVERSSDVPATHAAEYLNNEPSGTEEQDRSLPSPTPSQGQGHHQRQLSVLSRQGRKKIGNLLALVREAVNLKKDDVEQAGSDESPGPTTPTYLAYTPPPPPSVLSSVSSSTALEMPPTPEPESPTPSAPLHFGSSTSSRAPSKPPKPPMVPASATAPTATMDDLEELDTAGPITFPRRSDSHRRRTMRQDSQSSIWSDNIPTITISTTGSDECIVDAAAPQNGLPDPRSASPEPTVNIIRIDIENEHEK

Target ORF information:

RefSeq Version	NM_001259769.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transmembrane channel-like, transcript variant D (Tmc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259769.3

ORF Insert Sequence:

ATGCAGAGCA GCGCCGATGC CAAGAGGGAA TTCTACAAAG GACAGCGAGG CAGGAGAGCT 
GCGTCACAGG AGGACCACAG ATCCTACGAA CTGAACGATT TCCCATTGCA AAACCAGAGC 
AGCGACGCCG AGAGTTGCCA CCAGGAGCCT CACTTTGCCC ACCAGCGTTC GCCTGGAATC 
GGATTCGACG AGGATGGCGG CGGTGGCGAC ATTGACGATG AGGAGAGCTA CACAATTTCC 
GTATCGGCAA TAATGCAGAG ACGTGCCAGT GTGCGCGGCT ATCGGGGCAA ACGCGGCAGC 
CGGAGTTCGC GACGTGCATC CAGTCCCATG GATCACGTTC TGGACAGTGT GGAGCGACGA 
CGCTCCAGTG TCTATACGAC AAGTTCAGAG GAGGGCACCA ATCAGGAGTC AACGCAAGAG 
CAAATCTTTG AGAACATCCG CCTGCACAAG GAGGTCATAC AGTCGGTGAA GCTACAGCCA 
TGGCCCATCC GCAAGAAGCT CAAGTTGGTG CGGCAGGCCA AAACATATGT GGCACGCCAC 
GAGGGCGCGC TCCAGGAGCG CTTCGCCATG TCACGCAGCA CCAGAGACTT GTGGGCGAGG 
TTTAAAATTT TAATGGCGGC ACGATGGCGA CACTGGAAAC GTGAGACGGC CAGCTTCCTT 
ACCGTCCTCA TTCCCTGGGA GCTGCGCATC AAGGAAATCG AATCACACTT CGGCTCCGGC 
GTAGCCTCCT ACTTTACATT CCTGCGATGG CTTATGTGGG TCAATATCAT GATTGCCATT 
CCGCTGGTCG CCTTCGTCAT TGGCCCCGAG TACTTTGCCA CAAAGCACGG CGAAACGGAT 
CCCAGGAAAA GGATGTCCGA CCCGGAGGCT CGGGTGGCCG GCAATCTTTT CACGTTCTGG 
GAGTTCGAGG GATACCTGAA ATATTCACCT ATGTTTTATG GATACTATTC AAGCACATCC 
GGCATTAGCA CGTCCGGATA TAAGCTTCCC TTAGCCTATT TCCTTACCGC CGTTCTGGTC 
TACATCTACA GCTTTGTGGC CACGTTGAGG AAAATGGCGG AAAACTCGCG CAACTCAAAA 
CTTTCCTCGA AGGACGACGA GTGCGTCTTC TCGTGGAAGC TCTTCACCGG CTGGGACTTT 
ATGATTGGAC ACGCGGAAAC GGCGCACAAT CGCATCGCAT CCGTGGTCGT GGGCTTCAAG 
GAGGCACTGC TGGAGGAAGC GGAGAAAAAG AAGGATAATC GCAACTGGCG GGTCATACTC 
CAGAGGATAC TGGTCAATAT ACTGGTGATG GGGCTGCTGG GCTTGTCGGG CGCTACGGTC 
GTGCTGCTGG TTAATCACTC TGAGGATTTG GCCAAGCATG ACAACTGGCT GAGTCGCAAT 
GCGGTGAACG TGACCATGAC CCTGCTCTCC TTCTTCCTGC CCATGATATT CGAGGCACTG 
GGTCTGTTCG AGAACTGGCA TCCCAGGCAG CAGCTGCGTT TGCAATTGGC TCGCATTATG 
ATCCTGAACA TGTTAAACTT GTACTCGCTG ATGTTCTCCT TCATCTACAA GATCAACAGC 
AAGGAGAAAC CGCTACAGAT GTTGAAATTG GAGAACGAAA CAAACACCAT GGAGCTAAAG 
AATCTACTTA GTTCAATTGA GGCTCTGAGG GCCATGACAC CCACGACATC GCTATATGGT 
GAAAGTACTT CGGATGGGCT ATTCGATGAC TCTACATCAA CGGCAACTTG GGGTGAGGAT 
GGTGGTGGCC TCTTTTCGAC CACAGCGGCT GCTGCCTTAA TCTCCACCAC CGTGCAGCGT 
CTCAAGTGTT ACAACATGAC TGTAAAATGC TCAAAGCTGC GGCGCAACAT CATCAGTGGC 
AAGCACTTGG CCACCACTCT GATGGTTCTC AACCTGACAA CACCAGCCAT GGTGCCACCA 
ACTTTGCCAA CCACATTGCC CACCACATTT CCCACCACAT TGCCCACCAC ATTACCCACT 
ACATTACCTA CAGCATTGCC TACCACATTG CCCACCACAT TGCCTACTAC ATTGCCCTCC 
ACATTAGCCA CCACAACGGC GACGACTTCA AGTATTTGGT CAACCACAGA GGAAACATCT 
CCGACAACTA CAACGACATC ACCTTGGACC ACGTTACCGC CCTCGACAAC AACCACTGAG 
GCAACCACAA CGACTGAAAG AGCCACCACA ACCACAGAAG CAACGAGCAC AACCACACTG 
AAGATCACCA CTGCCGAAAT AAATAGTACA CTATCAGATA CTACAAAACC CCTGGGAAAA 
TCTATTGATA CTGAAATACC CAACTCAACA ACGAATTCTG CCACTTTGAG CACAATACCA 
GCCACTTTAA ACACCACTAA TTTACCCCTG AACAGCACCA CCAAACTAAC TACAACCACA 
TCCACGGAGA AGCCCCAGGG AGAGGATAAC TTTATCTACA CCACCGGAGA AGATGAGGGC 
TCCTACGATT ATGGCAGTGA TTCCACATCA GACGCTCCGG ATAATAATAG CTACTCCGAT 
ATAACGGATT ACTCTTCGGA ACCGTCGGAA ATTGAAGATT TCGATGAACA GGAAAGCACG 
GATCAGGCGG ACGATCCACT GGCCAAAGTA TTGGAGCAGC TCGATGAAAA TGAGACGAAG 
GGACGCAGGA AAAGGGCATT GGCCGAATCA CCATTCTTTA CCAGCAAGTA CAGCAGGCGA 
CATCGCAATG AATCCGCTGT CTCAGCTGGA CAACCACGAG AAACCACTGA ATCGGTGAAT 
GCCACACCCA GTCGGTGGCC ATTTAATTGG GCATCTTTTA GGCAAACCAC ACCGAGAACC 
ACCACAACAA GAAGAGTTCC CAGTGGAATT CTAACCAAGG AGGAATGGGA AAGACTCAGA 
CGACTTAGGG GCAGGATTAC CACAACTACC AGTACGTCCA CCACCAGCAC AACCACAAGA 
AGACCCAGGT GGAGATATCG AACCACGACT ACCGAACTTA CCAGCACCAC CGAGGAAGAA 
TCCTCCACAA CGGAGAGTTC CACTGATTCA TCGAGTCCAG GGAGCACTAC CAATGCCTTC 
GACAGCAGCA GTAGCACCAC CGAAGAAGAT GAATACACCA CCACGGAGGG ATCGGAGAAT 
AGACCCTACT ATGTGGGCTA CGTGGACATA TCGGAAATGG GAAGTACTAT CTACTACGAT 
GGAGATAGTG AATTTCTGGA GGAGTGTGTG ATTACCATTT GCCCCAAGGG CGATGACTTT 
TTTGGCAGCA CCACTGAAAG TCCGGATAGC ACCACCCAGA GCAGCGACTC CAAGCAGCTA 
ACCACAGTGA AGCTCACTCC GCTGGAGCGG AAGCAGAAGC GTTTGAAGGA AGTGCAGCTG 
GCGATTAAGC AAATACAAAC CAATCTGACG ACCATGTGCT GGGAGACCTC CTTGGGCCAG 
GAGCTCTCCA AAGTCATCGT TTTCGATGGG CTGATGTCCA TTGTGGCGCC GCTGTGCATC 
GACTTTCTGC GCGCCCTCTT CGTCCGGTAT GTCAATCAAA ACTGGTGCTG GGACATGGAG 
AAGACCTTTC CCCAGTACGG TGACTTCAAG ATAGCCGAGA ACATCCTGAC GTTGATTAAT 
AACCAGGGCC AAGTGTGGAT GGGCATATTC TTCTCGCCGG GCCTGGTGCT TATCAACCTG 
GTCAAATTGA TGATCATGAT GTACTTCCGT TCGTGGATAG TGCTCACCTG CAATGTGCCG 
CATGAGGTGG TGTTCAAGGC CTCCAAGTCG AACAACTTCT ACCTATCGCT GCTGTTGACC 
ATGCTGTTCC TTTGTGTCCT GCCCGTGGGC TATGCGATTG TGTGGCTGCG TCCTTCCTGG 
CATTGTGGAC CCTTCTCGGA GTACAACAGG ATAGCTGAGT TCATTACCAA CACCACGAGG 
AATGCTCTGC CAAAACAACT CCACGAACCC CTGGACTATC TGACCTCCTC GAGCACTGTG 
ATTCCATTGC TTCTGCTTCT TATCCTTATT ATATACTACC TGGTTTCGCT AACTGGCGCT 
CTACGCGAAG CCAACCAGGA TTTGCGCACC CAACTCCAGA AGGAACGCGA GGAGGAGCGC 
AAGAAGATCT TTAAGGTGCC GGAGGTCAAA CAAGCGGAAC CAACGGCCAC CACGCTGACA 
AATCGCTGGC GCAAAGTACT CGAGGCCAGC TCTCCGGTGA CACCCACCCA ACCACCCGAT 
TTCGATACGG AGGAGTACAA GAATCAAGCC CGGAAAGAGC TAATCTCACG CATCATGAAG 
AAGGCGTTGC GCAAGGGATC CGCCACGTCG GATGAGGATT CGTTTGTGCG GCGCGACGAC 
GATGACACGG ACACCGAGCA TCAGGACTCC CTGCCCCACG ACGAGGAGGC CAAGGACAAG 
CGGTTCGGCC TGTCGCGATT GCAGCAAATC CGGCGTACCC GCAAACCATC GCTGGTGGAC 
ATCGTTCAGA TTGCCAAGCA GGAGCGGGCA CGGGCGGGAT CCATTGTGGC CGGAACAAGC 
TCCTCTGGAA CTGGCAATTT TCCCATAAAG GAGACCCATC CAAAGAGCCG CTTCAAAGTG 
GAGAAACATG AGCGCAAGGA TAGGGGCAGC ATGAAGGATA AAAAGGACAC CAGGCACAGA 
CAGTCGCCAC AGCAGCAGCA ACAGCCGCCG CCCTATGAAT CGCCCAAGGA CAATGAACAT 
GATCCGGATA CAAATTCAAG AATTGTCAGC GCCAGTTTGC TGAGGCGCCA CAAGGAGCAG 
GCGGAGGGAG AGGAACCACC CACTACCCCC GACGCACCAC AAACACCCAA CTCGCCAGTA 
GAGCCCGTGG AACAGGCGCT CGAGGAATCC ACACCCGAAA CTCCCACGCT GGCCAAAAGC 
AAATTCCACA TAGTTGATGA AAAAAAACCC CCGCCGCATG AAGTGGAAGA TAAACCCTTG 
CCGACACCCA AGGAGAGTGG CAGTGGCGGT GGAAGTTTGG GCAAGTTCAA GTTCCGTAAG 
CACAAATTCA AGTCAAATAA TGTGGCTGCC GTCAAGCCGG AGCCAGAGGT GTTCAAGTTT 
GACGAGCGGA GTGTGGAAAG GAGTTCGGAT GTACCGGCAA CTCACGCCGC AGAGTACCTA 
AACAACGAGC CATCCGGCAC TGAGGAGCAG GATAGGAGTC TGCCATCACC CACTCCCAGC 
CAGGGTCAGG GTCACCACCA GCGGCAATTG TCTGTGCTTT CCCGGCAGGG TCGCAAGAAG 
ATCGGCAACC TGTTGGCCCT GGTTCGTGAA GCCGTCAATC TCAAAAAGGA CGATGTGGAG 
CAGGCGGGCT CGGATGAGTC GCCGGGTCCC ACAACGCCCA CCTACTTGGC CTACACACCG 
CCACCACCAC CATCAGTATT GTCCAGCGTG TCCAGTTCGA CGGCCTTGGA AATGCCACCT 
ACTCCGGAAC CGGAATCTCC AACGCCCAGT GCACCACTGC ACTTTGGCAG CAGCACATCG 
TCTCGTGCGC CTTCCAAGCC ACCAAAGCCT CCAATGGTTC CCGCATCCGC CACTGCTCCA 
ACGGCCACCA TGGATGACCT GGAGGAACTG GACACAGCCG GTCCTATTAC ATTTCCCAGG 
CGCAGCGATT CCCATCGCCG TCGCACTATG AGACAGGATT CACAGAGCTC CATTTGGTCG 
GATAATATAC CCACTATTAC TATAAGCACC ACCGGCAGCG ATGAGTGCAT TGTGGACGCA 
GCTGCTCCAC AAAATGGGCT TCCCGACCCG CGGAGTGCTT CCCCGGAGCC CACGGTCAAC 
ATTATAAGGA TTGACATTGA AAATGAGCAT GAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmc ( NM_001316433.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303362.1 Drosophila melanogaster transmembrane channel-like, transcript variant C (Tmc), mRNA.

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CloneID	OFa41364
Clone ID Related Accession (Same CDS sequence)	NM_001316433.1
Accession Version	NM_001316433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transmembrane channel-like, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAACG ACGAGGAACC AGCAGCTGCA GCCGGAACTT CGGGGCTAAG CAATGGAGAA 
TCCTTGCGGA GTCCACCGGC CCCGGCTCCA CGACGACCCA AGCCAGGTAT CCTGCGACTG 
GACATCGGCA AACCGCGACG CTCATCAGGT GGTTCCGTCG ATTTCCGATG CGTGGGCAGC 
AGCAGCAGCA ATGGGAACAC CAGCAACGTG GCCACTGGCG CCAACAGTGA GAACAACTCG 
GGAGTCACAT CGCCTCATCA ACTCTCTGTG ACCTGGGCAC CGCCATGCGA CTTGGATAGA 
GGTGGCTGGC AAATGCAGAG CAGCGCCGAT GCCAAGAGGG AATTCTACAA AGGACAGCGA 
GGCAGGAGAG CTGCGTCACA GGAGGACCAC AGATCCTACG AACTGAACGA TTTCCCATTG 
CAAAACCAGA GCAGCGACGC CGAGAGTTGC CACCAGGAGC CTCACTTTGC CCACCAGCGT 
TCGCCTGGAA TCGGATTCGA CGAGGATGGC GGCGGTGGCG ACATTGACGA TGAGGAGAGC 
TACACAATTT CCGTATCGGC AATAATGCAG AGACGTGCCA GTGTGCGCGG CTATCGGGGC 
AAACGCGGCA GCCGGAGTTC GCGACGTGCA TCCAGTCCCA TGGATCACGT TCTGGACAGT 
GTGGAGCGAC GACGCTCCAG TGTCTATACG ACAAGTTCAG AGGAGGGCAC CAATCAGGAG 
TCAACGCAAG AGCAAATCTT TGAGAACATC CGCCTGCACA AGGAGGTCAT ACAGTCGGTG 
AAGCTACAGC CATGGCCCAT CCGCAAGAAG CTCAAGTTGG TGCGGCAGGC CAAAACATAT 
GTGGCACGCC ACGAGGGCGC GCTCCAGGAG CGCTTCGCCA TGTCACGCAG CACCAGAGAC 
TTGTGGGCGA GGTTTAAAAT TTTAATGGCG GCACGATGGC GACACTGGAA ACGTGAGACG 
GCCAGCTTCC TTACCGTCCT CATTCCCTGG GAGCTGCGCA TCAAGGAAAT CGAATCACAC 
TTCGGCTCCG GCGTAGCCTC CTACTTTACA TTCCTGCGAT GGCTTATGTG GGTCAATATC 
ATGATTGCCA TTCCGCTGGT CGCCTTCGTC ATTGGCCCCG AGTACTTTGC CACAAAGCAC 
GGCGAAACGG ATCCCAGGAA AAGGATGTCC GACCCGGAGG CTCGGGTGGC CGGCAATCTT 
TTCACGTTCT GGGAGTTCGA GGGATACCTG AAATATTCAC CTATGTTTTA TGGATACTAT 
TCAAGCACAT CCGGCATTAG CACGTCCGGA TATAAGCTTC CCTTAGCCTA TTTCCTTACC 
GCCGTTCTGG TCTACATCTA CAGCTTTGTG GCCACGTTGA GGAAAATGGC GGAAAACTCG 
CGCAACTCAA AACTTTCCTC GAAGGACGAC GAGTGCGTCT TCTCGTGGAA GCTCTTCACC 
GGCTGGGACT TTATGATTGG ACACGCGGAA ACGGCGCACA ATCGCATCGC ATCCGTGGTC 
GTGGGCTTCA AGGAGGCACT GCTGGAGGAA GCGGAGAAAA AGAAGGATAA TCGCAACTGG 
CGGGTCATAC TCCAGAGGAT ACTGGTCAAT ATACTGGTGA TGGGGCTGCT GGGCTTGTCG 
GGCGCTACGG TCGTGCTGCT GGTTAATCAC TCTGAGGATT TGGCCAAGCA TGACAACTGG 
CTGAGTCGCA ATGCGGTGAA CGTGACCATG ACCCTGCTCT CCTTCTTCCT GCCCATGATA 
TTCGAGGCAC TGGGTCTGTT CGAGAACTGG CATCCCAGGC AGCAGCTGCG TTTGCAATTG 
GCTCGCATTA TGATCCTGAA CATGTTAAAC TTGTACTCGC TGATGTTCTC CTTCATCTAC 
AAGATCAACA GCAAGGAGAA ACCGCTACAG ATGTTGAAAT TGGAGAACGA AACAAACACC 
ATGGAGCTAA AGAATCTACT TAGTTCAATT GAGGCTCTGA GGGCCATGAC ACCCACGACA 
TCGCTATATG GTGAAAGTAC TTCGGATGGG CTATTCGATG ACTCTACATC AACGGCAACT 
TGGGGTGAGG ATGGTGGTGG CCTCTTTTCG ACCACAGCGG CTGCTGCCTT AATCTCCACC 
ACCGTGCAGC GTCTCAAGTG TTACAACATG ACTGTAAAAT GCTCAAAGCT GCGGCGCAAC 
ATCATCAGTG GCAAGCACTT GGCCACCACT CTGATGGTTC TCAACCTGAC AACACCAGCC 
ATGGTGCCAC CAACTTTGCC AACCACATTG CCCACCACAT TTCCCACCAC ATTGCCCACC 
ACATTACCCA CTACATTACC TACAGCATTG CCTACCACAT TGCCCACCAC ATTGCCTACT 
ACATTGCCCT CCACATTAGC CACCACAACG GCGACGACTT CAAGTATTTG GTCAACCACA 
GAGGAAACAT CTCCGACAAC TACAACGACA TCACCTTGGA CCACGTTACC GCCCTCGACA 
ACAACCACTG AGGCAACCAC AACGACTGAA AGAGCCACCA CAACCACAGA AGCAACGAGC 
ACAACCACAC TGAAGATCAC CACTGCCGAA ATAAATAGTA CACTATCAGA TACTACAAAA 
CCCCTGGGAA AATCTATTGA TACTGAAATA CCCAACTCAA CAACGAATTC TGCCACTTTG 
AGCACAATAC CAGCCACTTT AAACACCACT AATTTACCCC TGAACAGCAC CACCAAACTA 
ACTACAACCA CATCCACGGA GAAGCCCCAG GGAGAGGATA ACTTTATCTA CACCACCGGA 
GAAGATGAGG GCTCCTACGA TTATGGCAGT GATTCCACAT CAGACGCTCC GGATAATAAT 
AGCTACTCCG ATATAACGGA TTACTCTTCG GAACCGTCGG AAATTGAAGA TTTCGATGAA 
CAGGAAAGCA CGGATCAGGC GGACGATCCA CTGGCCAAAG TATTGGAGCA GCTCGATGAA 
AATGAGACGA AGGGACGCAG GAAAAGGGCA TTGGCCGAAT CACCATTCTT TACCAGCAAG 
TACAGCAGGC GACATCGCAA TGAATCCGCT GTCTCAGCTG GACAACCACG AGAAACCACT 
GAATCGGTGA ATGCCACACC CAGTCGGTGG CCATTTAATT GGGCATCTTT TAGGCAAACC 
ACACCGAGAA CCACCACAAC AAGAAGAGTT CCCAGTGGAA TTCTAACCAA GGAGGAATGG 
GAAAGACTCA GACGACTTAG GGGCAGGATT ACCACAACTA CCAGTACGTC CACCACCAGC 
ACAACCACAA GAAGACCCAG GTGGAGATAT CGAACCACGA CTACCGAACT TACCAGCACC 
ACCGAGGAAG AATCCTCCAC AACGGAGAGT TCCACTGATT CATCGAGTCC AGGGAGCACT 
ACCAATGCCT TCGACAGCAG CAGTAGCACC ACCGAAGAAG ATGAATACAC CACCACGGAG 
GGATCGGAGA ATAGACCCTA CTATGTGGGC TACGTGGACA TATCGGAAAT GGGAAGTACT 
ATCTACTACG ATGGAGATAG TGAATTTCTG GAGGAGTGTG TGATTACCAT TTGCCCCAAG 
GGCGATGACT TTTTTGGCAG CACCACTGAA AGTCCGGATA GCACCACCCA GAGCAGCGAC 
TCCAAGCAGC TAACCACAGT GAAGCTCACT CCGCTGGAGC GGAAGCAGAA GCGTTTGAAG 
GAAGTGCAGC TGGCGATTAA GCAAATACAA ACCAATCTGA CGACCATGTG CTGGGAGACC 
TCCTTGGGCC AGGAGCTCTC CAAAGTCATC GTTTTCGATG GGCTGATGTC CATTGTGGCG 
CCGCTGTGCA TCGACTTTCT GCGCGCCCTC TTCGTCCGGT ATGTCAATCA AAACTGGTGC 
TGGGACATGG AGAAGACCTT TCCCCAGTAC GGTGACTTCA AGATAGCCGA GAACATCCTG 
ACGTTGATTA ATAACCAGGG CCAAGTGTGG ATGGGCATAT TCTTCTCGCC GGGCCTGGTG 
CTTATCAACC TGGTCAAATT GATGATCATG ATGTACTTCC GTTCGTGGAT AGTGCTCACC 
TGCAATGTGC CGCATGAGGT GGTGTTCAAG GCCTCCAAGT CGAACAACTT CTACCTATCG 
CTGCTGTTGA CCATGCTGTT CCTTTGTGTC CTGCCCGTGG GCTATGCGAT TGTGTGGCTG 
CGTCCTTCCT GGCATTGTGG ACCCTTCTCG GAGTACAACA GGATAGCTGA GTTCATTACC 
AACACCACGA GGAATGCTCT GCCAAAACAA CTCCACGAAC CCCTGGACTA TCTGACCTCC 
TCGAGCACTG TGATTCCATT GCTTCTGCTT CTTATCCTTA TTATATACTA CCTGGTTTCG 
CTAACTGGCG CTCTACGCGA AGCCAACCAG GATTTGCGCA CCCAACTCCA GAAGGAACGC 
GAGGAGGAGC GCAAGAAGAT CTTTAAGGTG CCGGAGGTCA AACAAGCGGA ACCAACGGCC 
ACCACGCTGA CAAATCGCTG GCGCAAAGTA CTCGAGGCCA GCTCTCCGGT GACACCCACC 
CAACCACCCG ATTTCGATAC GGAGGAGTAC AAGAATCAAG CCCGGAAAGA GCTAATCTCA 
CGCATCATGA AGAAGGCGTT GCGCAAGGGA TCCGCCACGT CGGATGAGGA TTCGTTTGTG 
CGGCGCGACG ACGATGACAC GGACACCGAG CATCAGGACT CCCTGCCCCA CGACGAGGAG 
GCCAAGGACA AGCGGTTCGG CCTGTCGCGA TTGCAGCAAA TCCGGCGTAC CCGCAAACCA 
TCGCTGGTGG ACATCGTTCA GATTGCCAAG CAGGAGCGGG CACGGGCGGG ATCCATTGTG 
GCCGGAACAA GCTCCTCTGG AACTGGCAAT TTTCCCATAA AGGAGACCCA TCCAAAGAGC 
CGCTTCAAAG TGGAGAAACA TGAGCGCAAG GATAGGGGCA GCATGAAGGA TAAAAAGGAC 
ACCAGGCACA GACAGTCGCC ACAGCAGCAG CAACAGCCGC CGCCCTATGA ATCGCCCAAG 
GACAATGAAC ATGATCCGGA TACAAATTCA AGAATTGTCA GCGCCAGTTT GCTGAGGCGC 
CACAAGGAGC AGGCGGAGGG AGAGGAACCA CCCACTACCC CCGACGCACC ACAAACACCC 
AACTCGCCAG TAGAGCCCGT GGAACAGGCG CTCGAGGAAT CCACACCCGA AACTCCCACG 
CTGGCCAAAA GCAAATTCCA CATAGTTGAT GAAAAAAAAC CCCCGCCGCA TGAAGTGGAA 
GATAAACCCT TGCCGACACC CAAGGAGAGT GGCAGTGGCG GTGGAAGTTT GGGCAAGTTC 
AAGTTCCGTA AGCACAAATT CAAGTCAAAT AATGTGGCTG CCGTCAAGCC GGAGCCAGAG 
GTGTTCAAGT TTGACGAGCG GAGTGTGGAA AGGAGTTCGG ATGTACCGGC AACTCACGCC 
GCAGAGTACC TAAACAACGA GCCATCCGGC ACTGAGGAGC AGGATAGGAG TCTGCCATCA 
CCCACTCCCA GCCAGGGTCA GGGTCACCAC CAGCGGCAAT TGTCTGTGCT TTCCCGGCAG 
GGTCGCAAGA AGATCGGCAA CCTGTTGGCC CTGGTTCGTG AAGCCGTCAA TCTCAAAAAG 
GACGATGTGG AGCAGGCGGG CTCGGATGAG TCGCCGGGTC CCACAACGCC CACCTACTTG 
GCCTACACAC CGCCACCACC ACCATCAGTA TTGTCCAGCG TGTCCAGTTC GACGGCCTTG 
GAAATGCCAC CTACTCCGGA ACCGGAATCT CCAACGCCCA GTGCACCACT GCACTTTGGC 
AGCAGCACAT CGTCTCGTGC GCCTTCCAAG CCACCAAAGC CTCCAATGGT TCCCGCATCC 
GCCACTGCTC CAACGGCCAC CATGGATGAC CTGGAGGAAC TGGACACAGC CGGTCCTATT 
ACATTTCCCA GGCGCAGCGA TTCCCATCGC CGTCGCACTA TGAGACAGGA TTCACAGAGC 
TCCATTTGGT CGGATAATAT ACCCACTATT ACTATAAGCA CCACCGGCAG CGATGAGTGC 
ATTGTGGACG CAGCTGCTCC ACAAAATGGG CTTCCCGACC CGCGGAGTGC TTCCCCGGAG 
CCCACGGTCA ACATTATAAG GATTGACATT GAAAATGAGC ATGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303362.1
CDS	91..6201
Translation	MQNDEEPAAAAGTSGLSNGESLRSPPAPAPRRPKPGILRLDIGKPRRSSGGSVDFRCVGSSSSNGNTSNVATGANSENNSGVTSPHQLSVTWAPPCDLDRGGWQMQSSADAKREFYKGQRGRRAASQEDHRSYELNDFPLQNQSSDAESCHQEPHFAHQRSPGIGFDEDGGGGDIDDEESYTISVSAIMQRRASVRGYRGKRGSRSSRRASSPMDHVLDSVERRRSSVYTTSSEEGTNQESTQEQIFENIRLHKEVIQSVKLQPWPIRKKLKLVRQAKTYVARHEGALQERFAMSRSTRDLWARFKILMAARWRHWKRETASFLTVLIPWELRIKEIESHFGSGVASYFTFLRWLMWVNIMIAIPLVAFVIGPEYFATKHGETDPRKRMSDPEARVAGNLFTFWEFEGYLKYSPMFYGYYSSTSGISTSGYKLPLAYFLTAVLVYIYSFVATLRKMAENSRNSKLSSKDDECVFSWKLFTGWDFMIGHAETAHNRIASVVVGFKEALLEEAEKKKDNRNWRVILQRILVNILVMGLLGLSGATVVLLVNHSEDLAKHDNWLSRNAVNVTMTLLSFFLPMIFEALGLFENWHPRQQLRLQLARIMILNMLNLYSLMFSFIYKINSKEKPLQMLKLENETNTMELKNLLSSIEALRAMTPTTSLYGESTSDGLFDDSTSTATWGEDGGGLFSTTAAAALISTTVQRLKCYNMTVKCSKLRRNIISGKHLATTLMVLNLTTPAMVPPTLPTTLPTTFPTTLPTTLPTTLPTALPTTLPTTLPTTLPSTLATTTATTSSIWSTTEETSPTTTTTSPWTTLPPSTTTTEATTTTERATTTTEATSTTTLKITTAEINSTLSDTTKPLGKSIDTEIPNSTTNSATLSTIPATLNTTNLPLNSTTKLTTTTSTEKPQGEDNFIYTTGEDEGSYDYGSDSTSDAPDNNSYSDITDYSSEPSEIEDFDEQESTDQADDPLAKVLEQLDENETKGRRKRALAESPFFTSKYSRRHRNESAVSAGQPRETTESVNATPSRWPFNWASFRQTTPRTTTTRRVPSGILTKEEWERLRRLRGRITTTTSTSTTSTTTRRPRWRYRTTTTELTSTTEEESSTTESSTDSSSPGSTTNAFDSSSSTTEEDEYTTTEGSENRPYYVGYVDISEMGSTIYYDGDSEFLEECVITICPKGDDFFGSTTESPDSTTQSSDSKQLTTVKLTPLERKQKRLKEVQLAIKQIQTNLTTMCWETSLGQELSKVIVFDGLMSIVAPLCIDFLRALFVRYVNQNWCWDMEKTFPQYGDFKIAENILTLINNQGQVWMGIFFSPGLVLINLVKLMIMMYFRSWIVLTCNVPHEVVFKASKSNNFYLSLLLTMLFLCVLPVGYAIVWLRPSWHCGPFSEYNRIAEFITNTTRNALPKQLHEPLDYLTSSSTVIPLLLLLILIIYYLVSLTGALREANQDLRTQLQKEREEERKKIFKVPEVKQAEPTATTLTNRWRKVLEASSPVTPTQPPDFDTEEYKNQARKELISRIMKKALRKGSATSDEDSFVRRDDDDTDTEHQDSLPHDEEAKDKRFGLSRLQQIRRTRKPSLVDIVQIAKQERARAGSIVAGTSSSGTGNFPIKETHPKSRFKVEKHERKDRGSMKDKKDTRHRQSPQQQQQPPPYESPKDNEHDPDTNSRIVSASLLRRHKEQAEGEEPPTTPDAPQTPNSPVEPVEQALEESTPETPTLAKSKFHIVDEKKPPPHEVEDKPLPTPKESGSGGGSLGKFKFRKHKFKSNNVAAVKPEPEVFKFDERSVERSSDVPATHAAEYLNNEPSGTEEQDRSLPSPTPSQGQGHHQRQLSVLSRQGRKKIGNLLALVREAVNLKKDDVEQAGSDESPGPTTPTYLAYTPPPPPSVLSSVSSSTALEMPPTPEPESPTPSAPLHFGSSTSSRAPSKPPKPPMVPASATAPTATMDDLEELDTAGPITFPRRSDSHRRRTMRQDSQSSIWSDNIPTITISTTGSDECIVDAAAPQNGLPDPRSASPEPTVNIIRIDIENEHEK

Target ORF information:

RefSeq Version	NM_001316433.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transmembrane channel-like, transcript variant C (Tmc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316433.1

ORF Insert Sequence:

ATGCAGAACG ACGAGGAACC AGCAGCTGCA GCCGGAACTT CGGGGCTAAG CAATGGAGAA 
TCCTTGCGGA GTCCACCGGC CCCGGCTCCA CGACGACCCA AGCCAGGTAT CCTGCGACTG 
GACATCGGCA AACCGCGACG CTCATCAGGT GGTTCCGTCG ATTTCCGATG CGTGGGCAGC 
AGCAGCAGCA ATGGGAACAC CAGCAACGTG GCCACTGGCG CCAACAGTGA GAACAACTCG 
GGAGTCACAT CGCCTCATCA ACTCTCTGTG ACCTGGGCAC CGCCATGCGA CTTGGATAGA 
GGTGGCTGGC AAATGCAGAG CAGCGCCGAT GCCAAGAGGG AATTCTACAA AGGACAGCGA 
GGCAGGAGAG CTGCGTCACA GGAGGACCAC AGATCCTACG AACTGAACGA TTTCCCATTG 
CAAAACCAGA GCAGCGACGC CGAGAGTTGC CACCAGGAGC CTCACTTTGC CCACCAGCGT 
TCGCCTGGAA TCGGATTCGA CGAGGATGGC GGCGGTGGCG ACATTGACGA TGAGGAGAGC 
TACACAATTT CCGTATCGGC AATAATGCAG AGACGTGCCA GTGTGCGCGG CTATCGGGGC 
AAACGCGGCA GCCGGAGTTC GCGACGTGCA TCCAGTCCCA TGGATCACGT TCTGGACAGT 
GTGGAGCGAC GACGCTCCAG TGTCTATACG ACAAGTTCAG AGGAGGGCAC CAATCAGGAG 
TCAACGCAAG AGCAAATCTT TGAGAACATC CGCCTGCACA AGGAGGTCAT ACAGTCGGTG 
AAGCTACAGC CATGGCCCAT CCGCAAGAAG CTCAAGTTGG TGCGGCAGGC CAAAACATAT 
GTGGCACGCC ACGAGGGCGC GCTCCAGGAG CGCTTCGCCA TGTCACGCAG CACCAGAGAC 
TTGTGGGCGA GGTTTAAAAT TTTAATGGCG GCACGATGGC GACACTGGAA ACGTGAGACG 
GCCAGCTTCC TTACCGTCCT CATTCCCTGG GAGCTGCGCA TCAAGGAAAT CGAATCACAC 
TTCGGCTCCG GCGTAGCCTC CTACTTTACA TTCCTGCGAT GGCTTATGTG GGTCAATATC 
ATGATTGCCA TTCCGCTGGT CGCCTTCGTC ATTGGCCCCG AGTACTTTGC CACAAAGCAC 
GGCGAAACGG ATCCCAGGAA AAGGATGTCC GACCCGGAGG CTCGGGTGGC CGGCAATCTT 
TTCACGTTCT GGGAGTTCGA GGGATACCTG AAATATTCAC CTATGTTTTA TGGATACTAT 
TCAAGCACAT CCGGCATTAG CACGTCCGGA TATAAGCTTC CCTTAGCCTA TTTCCTTACC 
GCCGTTCTGG TCTACATCTA CAGCTTTGTG GCCACGTTGA GGAAAATGGC GGAAAACTCG 
CGCAACTCAA AACTTTCCTC GAAGGACGAC GAGTGCGTCT TCTCGTGGAA GCTCTTCACC 
GGCTGGGACT TTATGATTGG ACACGCGGAA ACGGCGCACA ATCGCATCGC ATCCGTGGTC 
GTGGGCTTCA AGGAGGCACT GCTGGAGGAA GCGGAGAAAA AGAAGGATAA TCGCAACTGG 
CGGGTCATAC TCCAGAGGAT ACTGGTCAAT ATACTGGTGA TGGGGCTGCT GGGCTTGTCG 
GGCGCTACGG TCGTGCTGCT GGTTAATCAC TCTGAGGATT TGGCCAAGCA TGACAACTGG 
CTGAGTCGCA ATGCGGTGAA CGTGACCATG ACCCTGCTCT CCTTCTTCCT GCCCATGATA 
TTCGAGGCAC TGGGTCTGTT CGAGAACTGG CATCCCAGGC AGCAGCTGCG TTTGCAATTG 
GCTCGCATTA TGATCCTGAA CATGTTAAAC TTGTACTCGC TGATGTTCTC CTTCATCTAC 
AAGATCAACA GCAAGGAGAA ACCGCTACAG ATGTTGAAAT TGGAGAACGA AACAAACACC 
ATGGAGCTAA AGAATCTACT TAGTTCAATT GAGGCTCTGA GGGCCATGAC ACCCACGACA 
TCGCTATATG GTGAAAGTAC TTCGGATGGG CTATTCGATG ACTCTACATC AACGGCAACT 
TGGGGTGAGG ATGGTGGTGG CCTCTTTTCG ACCACAGCGG CTGCTGCCTT AATCTCCACC 
ACCGTGCAGC GTCTCAAGTG TTACAACATG ACTGTAAAAT GCTCAAAGCT GCGGCGCAAC 
ATCATCAGTG GCAAGCACTT GGCCACCACT CTGATGGTTC TCAACCTGAC AACACCAGCC 
ATGGTGCCAC CAACTTTGCC AACCACATTG CCCACCACAT TTCCCACCAC ATTGCCCACC 
ACATTACCCA CTACATTACC TACAGCATTG CCTACCACAT TGCCCACCAC ATTGCCTACT 
ACATTGCCCT CCACATTAGC CACCACAACG GCGACGACTT CAAGTATTTG GTCAACCACA 
GAGGAAACAT CTCCGACAAC TACAACGACA TCACCTTGGA CCACGTTACC GCCCTCGACA 
ACAACCACTG AGGCAACCAC AACGACTGAA AGAGCCACCA CAACCACAGA AGCAACGAGC 
ACAACCACAC TGAAGATCAC CACTGCCGAA ATAAATAGTA CACTATCAGA TACTACAAAA 
CCCCTGGGAA AATCTATTGA TACTGAAATA CCCAACTCAA CAACGAATTC TGCCACTTTG 
AGCACAATAC CAGCCACTTT AAACACCACT AATTTACCCC TGAACAGCAC CACCAAACTA 
ACTACAACCA CATCCACGGA GAAGCCCCAG GGAGAGGATA ACTTTATCTA CACCACCGGA 
GAAGATGAGG GCTCCTACGA TTATGGCAGT GATTCCACAT CAGACGCTCC GGATAATAAT 
AGCTACTCCG ATATAACGGA TTACTCTTCG GAACCGTCGG AAATTGAAGA TTTCGATGAA 
CAGGAAAGCA CGGATCAGGC GGACGATCCA CTGGCCAAAG TATTGGAGCA GCTCGATGAA 
AATGAGACGA AGGGACGCAG GAAAAGGGCA TTGGCCGAAT CACCATTCTT TACCAGCAAG 
TACAGCAGGC GACATCGCAA TGAATCCGCT GTCTCAGCTG GACAACCACG AGAAACCACT 
GAATCGGTGA ATGCCACACC CAGTCGGTGG CCATTTAATT GGGCATCTTT TAGGCAAACC 
ACACCGAGAA CCACCACAAC AAGAAGAGTT CCCAGTGGAA TTCTAACCAA GGAGGAATGG 
GAAAGACTCA GACGACTTAG GGGCAGGATT ACCACAACTA CCAGTACGTC CACCACCAGC 
ACAACCACAA GAAGACCCAG GTGGAGATAT CGAACCACGA CTACCGAACT TACCAGCACC 
ACCGAGGAAG AATCCTCCAC AACGGAGAGT TCCACTGATT CATCGAGTCC AGGGAGCACT 
ACCAATGCCT TCGACAGCAG CAGTAGCACC ACCGAAGAAG ATGAATACAC CACCACGGAG 
GGATCGGAGA ATAGACCCTA CTATGTGGGC TACGTGGACA TATCGGAAAT GGGAAGTACT 
ATCTACTACG ATGGAGATAG TGAATTTCTG GAGGAGTGTG TGATTACCAT TTGCCCCAAG 
GGCGATGACT TTTTTGGCAG CACCACTGAA AGTCCGGATA GCACCACCCA GAGCAGCGAC 
TCCAAGCAGC TAACCACAGT GAAGCTCACT CCGCTGGAGC GGAAGCAGAA GCGTTTGAAG 
GAAGTGCAGC TGGCGATTAA GCAAATACAA ACCAATCTGA CGACCATGTG CTGGGAGACC 
TCCTTGGGCC AGGAGCTCTC CAAAGTCATC GTTTTCGATG GGCTGATGTC CATTGTGGCG 
CCGCTGTGCA TCGACTTTCT GCGCGCCCTC TTCGTCCGGT ATGTCAATCA AAACTGGTGC 
TGGGACATGG AGAAGACCTT TCCCCAGTAC GGTGACTTCA AGATAGCCGA GAACATCCTG 
ACGTTGATTA ATAACCAGGG CCAAGTGTGG ATGGGCATAT TCTTCTCGCC GGGCCTGGTG 
CTTATCAACC TGGTCAAATT GATGATCATG ATGTACTTCC GTTCGTGGAT AGTGCTCACC 
TGCAATGTGC CGCATGAGGT GGTGTTCAAG GCCTCCAAGT CGAACAACTT CTACCTATCG 
CTGCTGTTGA CCATGCTGTT CCTTTGTGTC CTGCCCGTGG GCTATGCGAT TGTGTGGCTG 
CGTCCTTCCT GGCATTGTGG ACCCTTCTCG GAGTACAACA GGATAGCTGA GTTCATTACC 
AACACCACGA GGAATGCTCT GCCAAAACAA CTCCACGAAC CCCTGGACTA TCTGACCTCC 
TCGAGCACTG TGATTCCATT GCTTCTGCTT CTTATCCTTA TTATATACTA CCTGGTTTCG 
CTAACTGGCG CTCTACGCGA AGCCAACCAG GATTTGCGCA CCCAACTCCA GAAGGAACGC 
GAGGAGGAGC GCAAGAAGAT CTTTAAGGTG CCGGAGGTCA AACAAGCGGA ACCAACGGCC 
ACCACGCTGA CAAATCGCTG GCGCAAAGTA CTCGAGGCCA GCTCTCCGGT GACACCCACC 
CAACCACCCG ATTTCGATAC GGAGGAGTAC AAGAATCAAG CCCGGAAAGA GCTAATCTCA 
CGCATCATGA AGAAGGCGTT GCGCAAGGGA TCCGCCACGT CGGATGAGGA TTCGTTTGTG 
CGGCGCGACG ACGATGACAC GGACACCGAG CATCAGGACT CCCTGCCCCA CGACGAGGAG 
GCCAAGGACA AGCGGTTCGG CCTGTCGCGA TTGCAGCAAA TCCGGCGTAC CCGCAAACCA 
TCGCTGGTGG ACATCGTTCA GATTGCCAAG CAGGAGCGGG CACGGGCGGG ATCCATTGTG 
GCCGGAACAA GCTCCTCTGG AACTGGCAAT TTTCCCATAA AGGAGACCCA TCCAAAGAGC 
CGCTTCAAAG TGGAGAAACA TGAGCGCAAG GATAGGGGCA GCATGAAGGA TAAAAAGGAC 
ACCAGGCACA GACAGTCGCC ACAGCAGCAG CAACAGCCGC CGCCCTATGA ATCGCCCAAG 
GACAATGAAC ATGATCCGGA TACAAATTCA AGAATTGTCA GCGCCAGTTT GCTGAGGCGC 
CACAAGGAGC AGGCGGAGGG AGAGGAACCA CCCACTACCC CCGACGCACC ACAAACACCC 
AACTCGCCAG TAGAGCCCGT GGAACAGGCG CTCGAGGAAT CCACACCCGA AACTCCCACG 
CTGGCCAAAA GCAAATTCCA CATAGTTGAT GAAAAAAAAC CCCCGCCGCA TGAAGTGGAA 
GATAAACCCT TGCCGACACC CAAGGAGAGT GGCAGTGGCG GTGGAAGTTT GGGCAAGTTC 
AAGTTCCGTA AGCACAAATT CAAGTCAAAT AATGTGGCTG CCGTCAAGCC GGAGCCAGAG 
GTGTTCAAGT TTGACGAGCG GAGTGTGGAA AGGAGTTCGG ATGTACCGGC AACTCACGCC 
GCAGAGTACC TAAACAACGA GCCATCCGGC ACTGAGGAGC AGGATAGGAG TCTGCCATCA 
CCCACTCCCA GCCAGGGTCA GGGTCACCAC CAGCGGCAAT TGTCTGTGCT TTCCCGGCAG 
GGTCGCAAGA AGATCGGCAA CCTGTTGGCC CTGGTTCGTG AAGCCGTCAA TCTCAAAAAG 
GACGATGTGG AGCAGGCGGG CTCGGATGAG TCGCCGGGTC CCACAACGCC CACCTACTTG 
GCCTACACAC CGCCACCACC ACCATCAGTA TTGTCCAGCG TGTCCAGTTC GACGGCCTTG 
GAAATGCCAC CTACTCCGGA ACCGGAATCT CCAACGCCCA GTGCACCACT GCACTTTGGC 
AGCAGCACAT CGTCTCGTGC GCCTTCCAAG CCACCAAAGC CTCCAATGGT TCCCGCATCC 
GCCACTGCTC CAACGGCCAC CATGGATGAC CTGGAGGAAC TGGACACAGC CGGTCCTATT 
ACATTTCCCA GGCGCAGCGA TTCCCATCGC CGTCGCACTA TGAGACAGGA TTCACAGAGC 
TCCATTTGGT CGGATAATAT ACCCACTATT ACTATAAGCA CCACCGGCAG CGATGAGTGC 
ATTGTGGACG CAGCTGCTCC ACAAAATGGG CTTCCCGACC CGCGGAGTGC TTCCCCGGAG 
CCCACGGTCA ACATTATAAG GATTGACATT GAAAATGAGC ATGAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tmc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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