bru2 gene cDNA ORF clone, Drosophila melanogaster(fruit fly)

mRNA	Protein	Name
NM_001042891.3	NP_001036356.1	bruno 2, isoform E
NM_176025.3	NP_788039.1	bruno 2, isoform D
NM_001259073.1	NP_001246002.1	bruno 2, isoform J
NM_001259069.2	NP_001245998.1	bruno 2, isoform F
NM_001259071.2	NP_001246000.1	bruno 2, isoform G
NM_001259072.1	NP_001246001.1	bruno 2, isoform I
NM_001273482.1	NP_001260411.1	bruno 2, isoform L
NM_135715.4	NP_609559.1	bruno 2, isoform A
NM_001259070.1	NP_001245999.1	bruno 2, isoform H
NM_001273481.1	NP_001260410.1	bruno 2, isoform K
NM_165007.3	NP_723742.2	bruno 2, isoform M

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15796	NM_001259072.1 Latest version!	Drosophila melanogaster bruno 2 (bru2), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa15386	NM_001042891.3 Latest version!	Drosophila melanogaster bruno 2 (bru2), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa15386	NM_001273482.1 Latest version!	Drosophila melanogaster bruno 2 (bru2), transcript variant L, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa15387	NM_176025.3 Latest version!	Drosophila melanogaster bruno 2 (bru2), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa15387	NM_001273481.1 Latest version!	Drosophila melanogaster bruno 2 (bru2), transcript variant K, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa15388	NM_135715.4 Latest version!	Drosophila melanogaster bruno 2 (bru2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa15389	NM_001259071.2 Latest version!	Drosophila melanogaster bruno 2 (bru2), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa15389	NM_165007.3 Latest version!	Drosophila melanogaster bruno 2 (bru2), transcript variant M, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa15794	NM_001259069.2 Latest version!	Drosophila melanogaster bruno 2 (bru2), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa15795	NM_001259070.1 Latest version!	Drosophila melanogaster bruno 2 (bru2), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa15795	NM_001259073.1 Latest version!	Drosophila melanogaster bruno 2 (bru2), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

bru2 ( NM_001259072.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246001.1 Drosophila melanogaster bruno 2 (bru2), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa15796
Clone ID Related Accession (Same CDS sequence)	NM_001259072.1
Accession Version	NM_001259072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bruno 2, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAACA AAAAGTACAC CGAGGCCGAT GTGAGGCAGC TCTTCACGGG CCACGGAACC 
ATCGAGGAGT GCACAGTGCT CCGGGATCAG GCTGGTCAGA GCAAGGGCTG CGCCTTCGTC 
ACATTCGCCA CTAAGCAGAA CGCCATTGGA GCTATAAAGG CCCTCCATCA AAGTCAGACC 
ATGGAAGGCT GTTCAGCTCC GCTGGTTGTG AAATTCGCCG ACACGCAAAA GGAAAAAGAC 
CAAAAGAAGA TGCAGCAGAT TCACGCATTC TGTGGCATCA ATACGCCGAG CGGTGCGACA 
GCAGGTGCGG CCACGCCCAC CATTAATGCC GCAACGGCTC TAATAGCTGC TCCGCCCTCG 
GCGGGACGTA CCAATCCGTC GATGGCGGCC GCACTGGCGG CAGTGCCTCA GGTTCAGCAG 
GCTGGTTCTG CAGCAACGGC ACCAACCACC CTCGTTCCCC TGAATTCCAC CACCGCCCTC 
AGTGCGTCCT TGACGCCCAA TCTCCTAGCC ACCAATGCCG CCCACCAGGG GGCGGCAGCT 
GCGGCCGCCT ATTTGGGGGC AGATCCCGCT GCGGCGGCCC ACCTTCAGCT GTACCAACAA 
CTACACGGAT ACGGACTGAG TCCGGCACAC TATCTGCCAG GGCCTCCAAA TGCCGCCGAC 
CCCTATTCAT CCTCGCTGAG CGGACTGACA AATGGAGCCG CCTACGGAGC CGCATCTCAA 
CCTGTGACCA CCAGCGCCCT TCAAGCGGCA GCTGCCGGCG TGACTGGCAA GCAGATCGAG 
GGTCCCGATG GCAGCAATCT TTTCATTTAT CACCTACCTC AGGAGTTCAT TGACACAGAT 
CTCGCGTCGA CGTTTCTTCC CTTCGGCAAT GTCCTTTCGG CCAAGGTGTT CATCGATAAG 
CAGACCAACC TGTCGAAGTG TTTCGGCTTT GTGTCCTACG ATAATCCCCA CTCGGCCAAC 
GCCGCTATCC AGGCGATGCA TGGCTTTCAA ATCGGCAGCA AGCGGCTGAA GGTCCAACTT 
AAACGTTCCA AGGATGCGGC TAAGCCGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246001.1
CDS	268..1320
Translation	MLNKKYTEADVRQLFTGHGTIEECTVLRDQAGQSKGCAFVTFATKQNAIGAIKALHQSQTMEGCSAPLVVKFADTQKEKDQKKMQQIHAFCGINTPSGATAGAATPTINAATALIAAPPSAGRTNPSMAAALAAVPQVQQAGSAATAPTTLVPLNSTTALSASLTPNLLATNAAHQGAAAAAAYLGADPAAAAHLQLYQQLHGYGLSPAHYLPGPPNAADPYSSSLSGLTNGAAYGAASQPVTTSALQAAAAGVTGKQIEGPDGSNLFIYHLPQEFIDTDLASTFLPFGNVLSAKVFIDKQTNLSKCFGFVSYDNPHSANAAIQAMHGFQIGSKRLKVQLKRSKDAAKPY

Target ORF information:

RefSeq Version	NM_001259072.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bruno 2 (bru2), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259072.1

ORF Insert Sequence:

ATGCTGAACA AAAAGTACAC CGAGGCCGAT GTGAGGCAGC TCTTCACGGG CCACGGAACC 
ATCGAGGAGT GCACAGTGCT CCGGGATCAG GCTGGTCAGA GCAAGGGCTG CGCCTTCGTC 
ACATTCGCCA CTAAGCAGAA CGCCATTGGA GCTATAAAGG CCCTCCATCA AAGTCAGACC 
ATGGAAGGCT GTTCAGCTCC GCTGGTTGTG AAATTCGCCG ACACGCAAAA GGAAAAAGAC 
CAAAAGAAGA TGCAGCAGAT TCACGCATTC TGTGGCATCA ATACGCCGAG CGGTGCGACA 
GCAGGTGCGG CCACGCCCAC CATTAATGCC GCAACGGCTC TAATAGCTGC TCCGCCCTCG 
GCGGGACGTA CCAATCCGTC GATGGCGGCC GCACTGGCGG CAGTGCCTCA GGTTCAGCAG 
GCTGGTTCTG CAGCAACGGC ACCAACCACC CTCGTTCCCC TGAATTCCAC CACCGCCCTC 
AGTGCGTCCT TGACGCCCAA TCTCCTAGCC ACCAATGCCG CCCACCAGGG GGCGGCAGCT 
GCGGCCGCCT ATTTGGGGGC AGATCCCGCT GCGGCGGCCC ACCTTCAGCT GTACCAACAA 
CTACACGGAT ACGGACTGAG TCCGGCACAC TATCTGCCAG GGCCTCCAAA TGCCGCCGAC 
CCCTATTCAT CCTCGCTGAG CGGACTGACA AATGGAGCCG CCTACGGAGC CGCATCTCAA 
CCTGTGACCA CCAGCGCCCT TCAAGCGGCA GCTGCCGGCG TGACTGGCAA GCAGATCGAG 
GGTCCCGATG GCAGCAATCT TTTCATTTAT CACCTACCTC AGGAGTTCAT TGACACAGAT 
CTCGCGTCGA CGTTTCTTCC CTTCGGCAAT GTCCTTTCGG CCAAGGTGTT CATCGATAAG 
CAGACCAACC TGTCGAAGTG TTTCGGCTTT GTGTCCTACG ATAATCCCCA CTCGGCCAAC 
GCCGCTATCC AGGCGATGCA TGGCTTTCAA ATCGGCAGCA AGCGGCTGAA GGTCCAACTT 
AAACGTTCCA AGGATGCGGC TAAGCCGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bru2 ( NM_001042891.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036356.1 Drosophila melanogaster bruno 2 (bru2), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa15386
Clone ID Related Accession (Same CDS sequence)	NM_001042891.3 , NM_001273482.1
Accession Version	NM_001042891.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bruno 2, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001042891.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GGACTAAATT TCCACCACCC ACCGGAAAAC AGTGCCCACC ACAGCCAGCA CAGTCCCGGC 
ATCGGCGGCG CATCAGCAGC ATCTCTATCG GCTGCAGCAG CCACAGCAGC ATCTAACCCA 
CTTGGCGGCG CACCACCACC CACACCCACA CCCACAAGTT CCGCCGGGCA TGCTGCTGGC 
GCTGGACTGC TCGCCGCTCC ATCCATGTCT ATGCAGAATC TGGTAGCCCT ACTAGCCACG 
CCCACCGCCC ACCACCAGCT GTCCCTCCAT CACAGCAGCA GCAGCAGCGG CCACAACAAC 
GGCAACAGCA GCGCCACCAG CGGCCTCCAC AACCAGCGGC TGACCTACGC GGCCGCACCA 
CCACCACCAC CCATCTCGAC CACCTACAAC ATGCCGCAGC TAATCGGTCA CACGGCCACG 
CCCACCCAGC CAGGGGGCAG CAGCCAATCG CTGACCTTCA ATGCGCTGTG GCCTCCAAAT 
GCCGCCGACC CCTATTCATC CTCGCTGAGC GGACTGACAA ATGGAGCCGC CTACGGAGCC 
GCATCTCAAC CTGTGACCAC CAGCGCCCTT CAAGCGGCAG CTGCCGGCGT GACTGGCAAG 
CAGATCGAGG GTCCCGATGG CAGCAATCTT TTCATTTATC ACCTACCTCA GGAGTTCATT 
GACACAGATC TCGCGTCGAC GTTTCTTCCC TTCGGCAATG TCCTTTCGGC CAAGGTGTTC 
ATCGATAAGC AGACCAACCT GTCGAAGTGT TTCGGCTTTG TGTCCTACGA TAATCCCCAC 
TCGGCCAACG CCGCTATCCA GGCGATGCAT GGCTTTCAAA TCGGCAGCAA GCGGCTGAAG 
GTCCAACTTA AACGTTCCAA GGATGCGGCT AAGCCGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036356.1
CDS	625..3306
Translation	MMLQSLSLHADKLSGNSPAAAAATGAAAATSEATAVASAAAATTMLATAPVAHVANGNNSNSSAGSSSNSNSSSNNHSLYSNNNLNTGNNSQQQQQQQQQHPQILPVKYEYLENLTSSGATSGAIAATTTFTGAAKSFHPYLRPTGSGSNSNNSIAALSTTTAATAAKMSTTLFETLLHNQINSSPIALPAATAIAAISAAATAATATPGTAATAAAAAAAAPSTQLNSSINSRTSLGDQGGAIVVVTPAPPVAAPPTGGPNRNQSPTRDCSVKMELMESTSPDSIKDQPDADNIKMFVGQIPKTWDETRLRQMFEQFGPVHTLNVLRDKVTSISRGCCFVTYYTRKAALRAQDALHNIKTLDGMHHPIQMKPADSENRNERKLFVGMLNKKYTEADVRQLFTGHGTIEECTVLRDQAGQSKGCAFVTFATKQNAIGAIKALHQSQTMEGCSAPLVVKFADTQKEKDQKKMQQIHAFCGINTPSGATAGAATPTINAATALIAAPPSAGRTNPSMAAALAAVPQVQQAGSAATAPTTLVPLNSTTALSASLTPNLLATNAAHQGAAAAAAYLGADPAAAAHLQLYQQLHGYGLSPAHYLPGLNFHHPPENSAHHSQHSPGIGGASAASLSAAAATAASNPLGGAPPPTPTPTSSAGHAAGAGLLAAPSMSMQNLVALLATPTAHHQLSLHHSSSSSGHNNGNSSATSGLHNQRLTYAAAPPPPPISTTYNMPQLIGHTATPTQPGGSSQSLTFNALWPPNAADPYSSSLSGLTNGAAYGAASQPVTTSALQAAAAGVTGKQIEGPDGSNLFIYHLPQEFIDTDLASTFLPFGNVLSAKVFIDKQTNLSKCFGFVSYDNPHSANAAIQAMHGFQIGSKRLKVQLKRSKDAAKPY

Target ORF information:

RefSeq Version	NM_001042891.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bruno 2 (bru2), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042891.3

ORF Insert Sequence:

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GGACTAAATT TCCACCACCC ACCGGAAAAC AGTGCCCACC ACAGCCAGCA CAGTCCCGGC 
ATCGGCGGCG CATCAGCAGC ATCTCTATCG GCTGCAGCAG CCACAGCAGC ATCTAACCCA 
CTTGGCGGCG CACCACCACC CACACCCACA CCCACAAGTT CCGCCGGGCA TGCTGCTGGC 
GCTGGACTGC TCGCCGCTCC ATCCATGTCT ATGCAGAATC TGGTAGCCCT ACTAGCCACG 
CCCACCGCCC ACCACCAGCT GTCCCTCCAT CACAGCAGCA GCAGCAGCGG CCACAACAAC 
GGCAACAGCA GCGCCACCAG CGGCCTCCAC AACCAGCGGC TGACCTACGC GGCCGCACCA 
CCACCACCAC CCATCTCGAC CACCTACAAC ATGCCGCAGC TAATCGGTCA CACGGCCACG 
CCCACCCAGC CAGGGGGCAG CAGCCAATCG CTGACCTTCA ATGCGCTGTG GCCTCCAAAT 
GCCGCCGACC CCTATTCATC CTCGCTGAGC GGACTGACAA ATGGAGCCGC CTACGGAGCC 
GCATCTCAAC CTGTGACCAC CAGCGCCCTT CAAGCGGCAG CTGCCGGCGT GACTGGCAAG 
CAGATCGAGG GTCCCGATGG CAGCAATCTT TTCATTTATC ACCTACCTCA GGAGTTCATT 
GACACAGATC TCGCGTCGAC GTTTCTTCCC TTCGGCAATG TCCTTTCGGC CAAGGTGTTC 
ATCGATAAGC AGACCAACCT GTCGAAGTGT TTCGGCTTTG TGTCCTACGA TAATCCCCAC 
TCGGCCAACG CCGCTATCCA GGCGATGCAT GGCTTTCAAA TCGGCAGCAA GCGGCTGAAG 
GTCCAACTTA AACGTTCCAA GGATGCGGCT AAGCCGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bru2 ( NM_001273482.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260411.1 Drosophila melanogaster bruno 2 (bru2), transcript variant L, mRNA.

Price & Availability↑

CloneID	OFa15386
Clone ID Related Accession (Same CDS sequence)	NM_001042891.3 , NM_001273482.1
Accession Version	NM_001273482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bruno 2, isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GGACTAAATT TCCACCACCC ACCGGAAAAC AGTGCCCACC ACAGCCAGCA CAGTCCCGGC 
ATCGGCGGCG CATCAGCAGC ATCTCTATCG GCTGCAGCAG CCACAGCAGC ATCTAACCCA 
CTTGGCGGCG CACCACCACC CACACCCACA CCCACAAGTT CCGCCGGGCA TGCTGCTGGC 
GCTGGACTGC TCGCCGCTCC ATCCATGTCT ATGCAGAATC TGGTAGCCCT ACTAGCCACG 
CCCACCGCCC ACCACCAGCT GTCCCTCCAT CACAGCAGCA GCAGCAGCGG CCACAACAAC 
GGCAACAGCA GCGCCACCAG CGGCCTCCAC AACCAGCGGC TGACCTACGC GGCCGCACCA 
CCACCACCAC CCATCTCGAC CACCTACAAC ATGCCGCAGC TAATCGGTCA CACGGCCACG 
CCCACCCAGC CAGGGGGCAG CAGCCAATCG CTGACCTTCA ATGCGCTGTG GCCTCCAAAT 
GCCGCCGACC CCTATTCATC CTCGCTGAGC GGACTGACAA ATGGAGCCGC CTACGGAGCC 
GCATCTCAAC CTGTGACCAC CAGCGCCCTT CAAGCGGCAG CTGCCGGCGT GACTGGCAAG 
CAGATCGAGG GTCCCGATGG CAGCAATCTT TTCATTTATC ACCTACCTCA GGAGTTCATT 
GACACAGATC TCGCGTCGAC GTTTCTTCCC TTCGGCAATG TCCTTTCGGC CAAGGTGTTC 
ATCGATAAGC AGACCAACCT GTCGAAGTGT TTCGGCTTTG TGTCCTACGA TAATCCCCAC 
TCGGCCAACG CCGCTATCCA GGCGATGCAT GGCTTTCAAA TCGGCAGCAA GCGGCTGAAG 
GTCCAACTTA AACGTTCCAA GGATGCGGCT AAGCCGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260411.1
CDS	532..3213
Translation	MMLQSLSLHADKLSGNSPAAAAATGAAAATSEATAVASAAAATTMLATAPVAHVANGNNSNSSAGSSSNSNSSSNNHSLYSNNNLNTGNNSQQQQQQQQQHPQILPVKYEYLENLTSSGATSGAIAATTTFTGAAKSFHPYLRPTGSGSNSNNSIAALSTTTAATAAKMSTTLFETLLHNQINSSPIALPAATAIAAISAAATAATATPGTAATAAAAAAAAPSTQLNSSINSRTSLGDQGGAIVVVTPAPPVAAPPTGGPNRNQSPTRDCSVKMELMESTSPDSIKDQPDADNIKMFVGQIPKTWDETRLRQMFEQFGPVHTLNVLRDKVTSISRGCCFVTYYTRKAALRAQDALHNIKTLDGMHHPIQMKPADSENRNERKLFVGMLNKKYTEADVRQLFTGHGTIEECTVLRDQAGQSKGCAFVTFATKQNAIGAIKALHQSQTMEGCSAPLVVKFADTQKEKDQKKMQQIHAFCGINTPSGATAGAATPTINAATALIAAPPSAGRTNPSMAAALAAVPQVQQAGSAATAPTTLVPLNSTTALSASLTPNLLATNAAHQGAAAAAAYLGADPAAAAHLQLYQQLHGYGLSPAHYLPGLNFHHPPENSAHHSQHSPGIGGASAASLSAAAATAASNPLGGAPPPTPTPTSSAGHAAGAGLLAAPSMSMQNLVALLATPTAHHQLSLHHSSSSSGHNNGNSSATSGLHNQRLTYAAAPPPPPISTTYNMPQLIGHTATPTQPGGSSQSLTFNALWPPNAADPYSSSLSGLTNGAAYGAASQPVTTSALQAAAAGVTGKQIEGPDGSNLFIYHLPQEFIDTDLASTFLPFGNVLSAKVFIDKQTNLSKCFGFVSYDNPHSANAAIQAMHGFQIGSKRLKVQLKRSKDAAKPY

Target ORF information:

RefSeq Version	NM_001273482.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bruno 2 (bru2), transcript variant L, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273482.1

ORF Insert Sequence:

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GGACTAAATT TCCACCACCC ACCGGAAAAC AGTGCCCACC ACAGCCAGCA CAGTCCCGGC 
ATCGGCGGCG CATCAGCAGC ATCTCTATCG GCTGCAGCAG CCACAGCAGC ATCTAACCCA 
CTTGGCGGCG CACCACCACC CACACCCACA CCCACAAGTT CCGCCGGGCA TGCTGCTGGC 
GCTGGACTGC TCGCCGCTCC ATCCATGTCT ATGCAGAATC TGGTAGCCCT ACTAGCCACG 
CCCACCGCCC ACCACCAGCT GTCCCTCCAT CACAGCAGCA GCAGCAGCGG CCACAACAAC 
GGCAACAGCA GCGCCACCAG CGGCCTCCAC AACCAGCGGC TGACCTACGC GGCCGCACCA 
CCACCACCAC CCATCTCGAC CACCTACAAC ATGCCGCAGC TAATCGGTCA CACGGCCACG 
CCCACCCAGC CAGGGGGCAG CAGCCAATCG CTGACCTTCA ATGCGCTGTG GCCTCCAAAT 
GCCGCCGACC CCTATTCATC CTCGCTGAGC GGACTGACAA ATGGAGCCGC CTACGGAGCC 
GCATCTCAAC CTGTGACCAC CAGCGCCCTT CAAGCGGCAG CTGCCGGCGT GACTGGCAAG 
CAGATCGAGG GTCCCGATGG CAGCAATCTT TTCATTTATC ACCTACCTCA GGAGTTCATT 
GACACAGATC TCGCGTCGAC GTTTCTTCCC TTCGGCAATG TCCTTTCGGC CAAGGTGTTC 
ATCGATAAGC AGACCAACCT GTCGAAGTGT TTCGGCTTTG TGTCCTACGA TAATCCCCAC 
TCGGCCAACG CCGCTATCCA GGCGATGCAT GGCTTTCAAA TCGGCAGCAA GCGGCTGAAG 
GTCCAACTTA AACGTTCCAA GGATGCGGCT AAGCCGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bru2 ( NM_176025.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788039.1 Drosophila melanogaster bruno 2 (bru2), transcript variant D, mRNA.

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CloneID	OFa15387
Clone ID Related Accession (Same CDS sequence)	NM_176025.3 , NM_001273481.1
Accession Version	NM_176025.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bruno 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_176025.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GGGCCTCCAA ATGCCGCCGA CCCCTATTCA TCCTCGCTGA GCGGACTGAC AAATGGAGCC 
GCCTACGGAG CCGCATCTCA ACCTGTGACC ACCAGCGCCC TTCAAGCGGC AGCTGCCGGC 
GTGACTGGCA AGCAGATCGA GGGTCCCGAT GGCAGCAATC TTTTCATTTA TCACCTACCT 
CAGGAGTTCA TTGACACAGA TCTCGCGTCG ACGTTTCTTC CCTTCGGCAA TGTCCTTTCG 
GCCAAGGTGT TCATCGATAA GCAGACCAAC CTGTCGAAGT GTTTCGGCTT TGTGTCCTAC 
GATAATCCCC ACTCGGCCAA CGCCGCTATC CAGGCGATGC ATGGCTTTCA AATCGGCAGC 
AAGCGGCTGA AGGTCCAACT TAAACGTTCC AAGGATGCGG CTAAGCCGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788039.1
CDS	625..2838
Translation	MMLQSLSLHADKLSGNSPAAAAATGAAAATSEATAVASAAAATTMLATAPVAHVANGNNSNSSAGSSSNSNSSSNNHSLYSNNNLNTGNNSQQQQQQQQQHPQILPVKYEYLENLTSSGATSGAIAATTTFTGAAKSFHPYLRPTGSGSNSNNSIAALSTTTAATAAKMSTTLFETLLHNQINSSPIALPAATAIAAISAAATAATATPGTAATAAAAAAAAPSTQLNSSINSRTSLGDQGGAIVVVTPAPPVAAPPTGGPNRNQSPTRDCSVKMELMESTSPDSIKDQPDADNIKMFVGQIPKTWDETRLRQMFEQFGPVHTLNVLRDKVTSISRGCCFVTYYTRKAALRAQDALHNIKTLDGMHHPIQMKPADSENRNERKLFVGMLNKKYTEADVRQLFTGHGTIEECTVLRDQAGQSKGCAFVTFATKQNAIGAIKALHQSQTMEGCSAPLVVKFADTQKEKDQKKMQQIHAFCGINTPSGATAGAATPTINAATALIAAPPSAGRTNPSMAAALAAVPQVQQAGSAATAPTTLVPLNSTTALSASLTPNLLATNAAHQGAAAAAAYLGADPAAAAHLQLYQQLHGYGLSPAHYLPGPPNAADPYSSSLSGLTNGAAYGAASQPVTTSALQAAAAGVTGKQIEGPDGSNLFIYHLPQEFIDTDLASTFLPFGNVLSAKVFIDKQTNLSKCFGFVSYDNPHSANAAIQAMHGFQIGSKRLKVQLKRSKDAAKPY

Target ORF information:

RefSeq Version	NM_176025.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bruno 2 (bru2), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176025.3

ORF Insert Sequence:

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GGGCCTCCAA ATGCCGCCGA CCCCTATTCA TCCTCGCTGA GCGGACTGAC AAATGGAGCC 
GCCTACGGAG CCGCATCTCA ACCTGTGACC ACCAGCGCCC TTCAAGCGGC AGCTGCCGGC 
GTGACTGGCA AGCAGATCGA GGGTCCCGAT GGCAGCAATC TTTTCATTTA TCACCTACCT 
CAGGAGTTCA TTGACACAGA TCTCGCGTCG ACGTTTCTTC CCTTCGGCAA TGTCCTTTCG 
GCCAAGGTGT TCATCGATAA GCAGACCAAC CTGTCGAAGT GTTTCGGCTT TGTGTCCTAC 
GATAATCCCC ACTCGGCCAA CGCCGCTATC CAGGCGATGC ATGGCTTTCA AATCGGCAGC 
AAGCGGCTGA AGGTCCAACT TAAACGTTCC AAGGATGCGG CTAAGCCGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bru2 ( NM_001273481.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260410.1 Drosophila melanogaster bruno 2 (bru2), transcript variant K, mRNA.

Price & Availability↑

CloneID	OFa15387
Clone ID Related Accession (Same CDS sequence)	NM_176025.3 , NM_001273481.1
Accession Version	NM_001273481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bruno 2, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GGGCCTCCAA ATGCCGCCGA CCCCTATTCA TCCTCGCTGA GCGGACTGAC AAATGGAGCC 
GCCTACGGAG CCGCATCTCA ACCTGTGACC ACCAGCGCCC TTCAAGCGGC AGCTGCCGGC 
GTGACTGGCA AGCAGATCGA GGGTCCCGAT GGCAGCAATC TTTTCATTTA TCACCTACCT 
CAGGAGTTCA TTGACACAGA TCTCGCGTCG ACGTTTCTTC CCTTCGGCAA TGTCCTTTCG 
GCCAAGGTGT TCATCGATAA GCAGACCAAC CTGTCGAAGT GTTTCGGCTT TGTGTCCTAC 
GATAATCCCC ACTCGGCCAA CGCCGCTATC CAGGCGATGC ATGGCTTTCA AATCGGCAGC 
AAGCGGCTGA AGGTCCAACT TAAACGTTCC AAGGATGCGG CTAAGCCGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260410.1
CDS	908..3121
Translation	MMLQSLSLHADKLSGNSPAAAAATGAAAATSEATAVASAAAATTMLATAPVAHVANGNNSNSSAGSSSNSNSSSNNHSLYSNNNLNTGNNSQQQQQQQQQHPQILPVKYEYLENLTSSGATSGAIAATTTFTGAAKSFHPYLRPTGSGSNSNNSIAALSTTTAATAAKMSTTLFETLLHNQINSSPIALPAATAIAAISAAATAATATPGTAATAAAAAAAAPSTQLNSSINSRTSLGDQGGAIVVVTPAPPVAAPPTGGPNRNQSPTRDCSVKMELMESTSPDSIKDQPDADNIKMFVGQIPKTWDETRLRQMFEQFGPVHTLNVLRDKVTSISRGCCFVTYYTRKAALRAQDALHNIKTLDGMHHPIQMKPADSENRNERKLFVGMLNKKYTEADVRQLFTGHGTIEECTVLRDQAGQSKGCAFVTFATKQNAIGAIKALHQSQTMEGCSAPLVVKFADTQKEKDQKKMQQIHAFCGINTPSGATAGAATPTINAATALIAAPPSAGRTNPSMAAALAAVPQVQQAGSAATAPTTLVPLNSTTALSASLTPNLLATNAAHQGAAAAAAYLGADPAAAAHLQLYQQLHGYGLSPAHYLPGPPNAADPYSSSLSGLTNGAAYGAASQPVTTSALQAAAAGVTGKQIEGPDGSNLFIYHLPQEFIDTDLASTFLPFGNVLSAKVFIDKQTNLSKCFGFVSYDNPHSANAAIQAMHGFQIGSKRLKVQLKRSKDAAKPY

Target ORF information:

RefSeq Version	NM_001273481.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bruno 2 (bru2), transcript variant K, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273481.1

ORF Insert Sequence:

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GGGCCTCCAA ATGCCGCCGA CCCCTATTCA TCCTCGCTGA GCGGACTGAC AAATGGAGCC 
GCCTACGGAG CCGCATCTCA ACCTGTGACC ACCAGCGCCC TTCAAGCGGC AGCTGCCGGC 
GTGACTGGCA AGCAGATCGA GGGTCCCGAT GGCAGCAATC TTTTCATTTA TCACCTACCT 
CAGGAGTTCA TTGACACAGA TCTCGCGTCG ACGTTTCTTC CCTTCGGCAA TGTCCTTTCG 
GCCAAGGTGT TCATCGATAA GCAGACCAAC CTGTCGAAGT GTTTCGGCTT TGTGTCCTAC 
GATAATCCCC ACTCGGCCAA CGCCGCTATC CAGGCGATGC ATGGCTTTCA AATCGGCAGC 
AAGCGGCTGA AGGTCCAACT TAAACGTTCC AAGGATGCGG CTAAGCCGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bru2 ( NM_135715.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609559.1 Drosophila melanogaster bruno 2 (bru2), transcript variant A, mRNA.

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CloneID	OFa15388
Clone ID Related Accession (Same CDS sequence)	NM_135715.4
Accession Version	NM_135715.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bruno 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135715.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GATGAGTCAA AAAAAAGTAC AAAGGAAAAG AAAAAAACGT CTTCGTCTAC CGTTTTCCCT 
GCTTTTCTTT GCCCATTTTC CGACTTGCAA TTTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609559.1
CDS	532..2430
Translation	MMLQSLSLHADKLSGNSPAAAAATGAAAATSEATAVASAAAATTMLATAPVAHVANGNNSNSSAGSSSNSNSSSNNHSLYSNNNLNTGNNSQQQQQQQQQHPQILPVKYEYLENLTSSGATSGAIAATTTFTGAAKSFHPYLRPTGSGSNSNNSIAALSTTTAATAAKMSTTLFETLLHNQINSSPIALPAATAIAAISAAATAATATPGTAATAAAAAAAAPSTQLNSSINSRTSLGDQGGAIVVVTPAPPVAAPPTGGPNRNQSPTRDCSVKMELMESTSPDSIKDQPDADNIKMFVGQIPKTWDETRLRQMFEQFGPVHTLNVLRDKVTSISRGCCFVTYYTRKAALRAQDALHNIKTLDGMHHPIQMKPADSENRNERKLFVGMLNKKYTEADVRQLFTGHGTIEECTVLRDQAGQSKGCAFVTFATKQNAIGAIKALHQSQTMEGCSAPLVVKFADTQKEKDQKKMQQIHAFCGINTPSGATAGAATPTINAATALIAAPPSAGRTNPSMAAALAAVPQVQQAGSAATAPTTLVPLNSTTALSASLTPNLLATNAAHQGAAAAAAYLGADPAAAAHLQLYQQLHGYGLSPAHYLPDESKKSTKEKKKTSSSTVFPAFLCPFSDLQFL

Target ORF information:

RefSeq Version	NM_135715.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bruno 2 (bru2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135715.4

ORF Insert Sequence:

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GATGAGTCAA AAAAAAGTAC AAAGGAAAAG AAAAAAACGT CTTCGTCTAC CGTTTTCCCT 
GCTTTTCTTT GCCCATTTTC CGACTTGCAA TTTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bru2 ( NM_001259071.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246000.1 Drosophila melanogaster bruno 2 (bru2), transcript variant G, mRNA.

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CloneID	OFa15389
Clone ID Related Accession (Same CDS sequence)	NM_001259071.2 , NM_165007.3
Accession Version	NM_001259071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bruno 2, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259071.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GTGCCCACCA CAGCCAGCAC AGTCCCGGCA TCGGCGGCGC ATCAGCAGCA TCTCTATCGG 
CTGCAGCAGC CACAGCAGCA TCTAACCCAC TTGGCGGCGC ACCACCACCC ACACCCACAC 
CCACAAGTTC CGCCGGGCAT GCTGCTGGCG CTGGACTGCT CGCCGCTCCA TCCATGTCTA 
TGCAGAATCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246000.1
CDS	625..2619
Translation	MMLQSLSLHADKLSGNSPAAAAATGAAAATSEATAVASAAAATTMLATAPVAHVANGNNSNSSAGSSSNSNSSSNNHSLYSNNNLNTGNNSQQQQQQQQQHPQILPVKYEYLENLTSSGATSGAIAATTTFTGAAKSFHPYLRPTGSGSNSNNSIAALSTTTAATAAKMSTTLFETLLHNQINSSPIALPAATAIAAISAAATAATATPGTAATAAAAAAAAPSTQLNSSINSRTSLGDQGGAIVVVTPAPPVAAPPTGGPNRNQSPTRDCSVKMELMESTSPDSIKDQPDADNIKMFVGQIPKTWDETRLRQMFEQFGPVHTLNVLRDKVTSISRGCCFVTYYTRKAALRAQDALHNIKTLDGMHHPIQMKPADSENRNERKLFVGMLNKKYTEADVRQLFTGHGTIEECTVLRDQAGQSKGCAFVTFATKQNAIGAIKALHQSQTMEGCSAPLVVKFADTQKEKDQKKMQQIHAFCGINTPSGATAGAATPTINAATALIAAPPSAGRTNPSMAAALAAVPQVQQAGSAATAPTTLVPLNSTTALSASLTPNLLATNAAHQGAAAAAAYLGADPAAAAHLQLYQQLHGYGLSPAHYLPVPTTASTVPASAAHQQHLYRLQQPQQHLTHLAAHHHPHPHPQVPPGMLLALDCSPLHPCLCRIW

Target ORF information:

RefSeq Version	NM_001259071.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bruno 2 (bru2), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259071.2

ORF Insert Sequence:

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GTGCCCACCA CAGCCAGCAC AGTCCCGGCA TCGGCGGCGC ATCAGCAGCA TCTCTATCGG 
CTGCAGCAGC CACAGCAGCA TCTAACCCAC TTGGCGGCGC ACCACCACCC ACACCCACAC 
CCACAAGTTC CGCCGGGCAT GCTGCTGGCG CTGGACTGCT CGCCGCTCCA TCCATGTCTA 
TGCAGAATCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bru2 ( NM_165007.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723742.2 Drosophila melanogaster bruno 2 (bru2), transcript variant M, mRNA.

Price & Availability↑

CloneID	OFa15389
Clone ID Related Accession (Same CDS sequence)	NM_001259071.2 , NM_165007.3
Accession Version	NM_165007.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bruno 2, isoform M
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165007.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GTGCCCACCA CAGCCAGCAC AGTCCCGGCA TCGGCGGCGC ATCAGCAGCA TCTCTATCGG 
CTGCAGCAGC CACAGCAGCA TCTAACCCAC TTGGCGGCGC ACCACCACCC ACACCCACAC 
CCACAAGTTC CGCCGGGCAT GCTGCTGGCG CTGGACTGCT CGCCGCTCCA TCCATGTCTA 
TGCAGAATCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723742.2
CDS	532..2526
Translation	MMLQSLSLHADKLSGNSPAAAAATGAAAATSEATAVASAAAATTMLATAPVAHVANGNNSNSSAGSSSNSNSSSNNHSLYSNNNLNTGNNSQQQQQQQQQHPQILPVKYEYLENLTSSGATSGAIAATTTFTGAAKSFHPYLRPTGSGSNSNNSIAALSTTTAATAAKMSTTLFETLLHNQINSSPIALPAATAIAAISAAATAATATPGTAATAAAAAAAAPSTQLNSSINSRTSLGDQGGAIVVVTPAPPVAAPPTGGPNRNQSPTRDCSVKMELMESTSPDSIKDQPDADNIKMFVGQIPKTWDETRLRQMFEQFGPVHTLNVLRDKVTSISRGCCFVTYYTRKAALRAQDALHNIKTLDGMHHPIQMKPADSENRNERKLFVGMLNKKYTEADVRQLFTGHGTIEECTVLRDQAGQSKGCAFVTFATKQNAIGAIKALHQSQTMEGCSAPLVVKFADTQKEKDQKKMQQIHAFCGINTPSGATAGAATPTINAATALIAAPPSAGRTNPSMAAALAAVPQVQQAGSAATAPTTLVPLNSTTALSASLTPNLLATNAAHQGAAAAAAYLGADPAAAAHLQLYQQLHGYGLSPAHYLPVPTTASTVPASAAHQQHLYRLQQPQQHLTHLAAHHHPHPHPQVPPGMLLALDCSPLHPCLCRIW

Target ORF information:

RefSeq Version	NM_165007.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bruno 2 (bru2), transcript variant M, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165007.3

ORF Insert Sequence:

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT 
GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG 
ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT 
GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG 
CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG 
AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG 
GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC 
GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC 
AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT 
CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG 
GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC 
CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC 
GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC 
CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA 
GTGCCCACCA CAGCCAGCAC AGTCCCGGCA TCGGCGGCGC ATCAGCAGCA TCTCTATCGG 
CTGCAGCAGC CACAGCAGCA TCTAACCCAC TTGGCGGCGC ACCACCACCC ACACCCACAC 
CCACAAGTTC CGCCGGGCAT GCTGCTGGCG CTGGACTGCT CGCCGCTCCA TCCATGTCTA 
TGCAGAATCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bru2 ( NM_001259069.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245998.1 Drosophila melanogaster bruno 2 (bru2), transcript variant F, mRNA.

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CloneID	OFa15794
Clone ID Related Accession (Same CDS sequence)	NM_001259069.2
Accession Version	NM_001259069.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bruno 2, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259069.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGA TGCACCATCC 
CATTCAGATG AAGCCGGCGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245998.1
CDS	532..1575
Translation	MMLQSLSLHADKLSGNSPAAAAATGAAAATSEATAVASAAAATTMLATAPVAHVANGNNSNSSAGSSSNSNSSSNNHSLYSNNNLNTGNNSQQQQQQQQQHPQILPVKYEYLENLTSSGATSGAIAATTTFTGAAKSFHPYLRPTGSGSNSNNSIAALSTTTAATAAKMSTTLFETLLHNQINSSPIALPAATAIAAISAAATAATATPGTAATAAAAAAAAPSTQLNSSINSRTSLGDQGGAIVVVTPAPPVAAPPTGGPNRNQSPTRDCSVKMELMESTSPDSIKDQPDADNIKMFVGQIPKTWDETRLRQMFEQFGPVHTLNVLRDKVTSISRDAPSHSDEAGG

Target ORF information:

RefSeq Version	NM_001259069.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bruno 2 (bru2), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259069.2

ORF Insert Sequence:

ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA 
GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC 
AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC 
AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA 
ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC 
TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA 
ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC 
CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA 
GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA 
GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG 
GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC 
CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG 
ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC 
CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC 
GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGA TGCACCATCC 
CATTCAGATG AAGCCGGCGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bru2 ( NM_001259073.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246002.1 Drosophila melanogaster bruno 2 (bru2), transcript variant J, mRNA.

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CloneID	OFa15795
Clone ID Related Accession (Same CDS sequence)	NM_001259073.1 , NM_001259070.1
Accession Version	NM_001259073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bruno 2, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAACC CAAATATCAT GGAATGCGGC AATTCCTTAA ACAACAACAA CATTGGCGGC 
AATATCAACG GAAACGGAAA TGGAAATGGC GGAGTCAGCG AACAACGCCC CCTATCGGGC 
AAAGTCCATG CTGCATCTCC TCCGCCCGTC GTCGGCAACA ACAACAACAA CAATAGTAAT 
CTAATAGTCC GTTACAACAA ACTAAGCTCG GGCAGCTCCT CCGCGAGCTC ATCGCCGCCA 
CATCATCAGC AGCACTCGCC ACCACCGTCG ATGCACCACC AAAGCCATCC CAACCACCCA 
CTCCAGCCGT CCTCCATGTC CACAAACATG GCCACAAATG GCCATCAGTT GTATCCGGCG 
ATAACGGCAA CGTATTGGCT CCCGCCGCCG AATCCGGCGC CATATTTAGT TCCAGAACGT 
AAACTTTTCG TTGGCATGCT GAACAAAAAG TACACCGAGG CCGATGTGAG GCAGCTCTTC 
ACGGGCCACG GAACCATCGA GGAGTGCACA GTGCTCCGGG ATCAGGCTGG TCAGAGCAAG 
GGCTGCGCCT TCGTCACATT CGCCACTAAG CAGAACGCCA TTGGAGCTAT AAAGGCCCTC 
CATCAAAGTC AGACCATGGA AGGCTGTTCA GCTCCGCTGG TTGTGAAATT CGCCGACACG 
CAAAAGGAAA AAGACCAAAA GAAGATGCAG CAGATTCACG CATTCTGTGG CATCAATACG 
CCGAGCGGTG CGACAGCAGG TGCGGCCACG CCCACCATTA ATGCCGCAAC GGCTCTAATA 
GCTGCTCCGC CCTCGGCGGG ACGTACCAAT CCGTCGATGG CGGCCGCACT GGCGGCAGTG 
CCTCAGGTTC AGCAGGCTGG TTCTGCAGCA ACGGCACCAA CCACCCTCGT TCCCCTGAAT 
TCCACCACCG CCCTCAGTGC GTCCTTGACG CCCAATCTCC TAGCCACCAA TGCCGCCCAC 
CAGGGGGCGG CAGCTGCGGC CGCCTATTTG GGGGCAGATC CCGCTGCGGC GGCCCACCTT 
CAGCTGTACC AACAACTACA CGGATACGGA CTGAGTCCGG CACACTATCT GCCAGGACTA 
AATTTCCACC ACCCACCGGA AAACAGTGCC CACCACAGCC AGCACAGTCC CGGCATCGGC 
GGCGCATCAG CAGCATCTCT ATCGGCTGCA GCAGCCACAG CAGCATCTAA CCCACTTGGC 
GGCGCACCAC CACCCACACC CACACCCACA AGTTCCGCCG GGCATGCTGC TGGCGCTGGA 
CTGCTCGCCG CTCCATCCAT GTCTATGCAG AATCTGGTAG CCCTACTAGC CACGCCCACC 
GCCCACCACC AGCTGTCCCT CCATCACAGC AGCAGCAGCA GCGGCCACAA CAACGGCAAC 
AGCAGCGCCA CCAGCGGCCT CCACAACCAG CGGCTGACCT ACGCGGCCGC ACCACCACCA 
CCACCCATCT CGACCACCTA CAACATGCCG CAGCTAATCG GTCACACGGC CACGCCCACC 
CAGCCAGGGG GCAGCAGCCA ATCGCTGACC TTCAATGCGC TGTGGCCTCC AAATGCCGCC 
GACCCCTATT CATCCTCGCT GAGCGGACTG ACAAATGGAG CCGCCTACGG AGCCGCATCT 
CAACCTGTGA CCACCAGCGC CCTTCAAGCG GCAGCTGCCG GCGTGACTGG CAAGCAGATC 
GAGGGTCCCG ATGGCAGCAA TCTTTTCATT TATCACCTAC CTCAGGAGTT CATTGACACA 
GATCTCGCGT CGACGTTTCT TCCCTTCGGC AATGTCCTTT CGGCCAAGGT GTTCATCGAT 
AAGCAGACCA ACCTGTCGAA GTGTTTCGGC TTTGTGTCCT ACGATAATCC CCACTCGGCC 
AACGCCGCTA TCCAGGCGAT GCATGGCTTT CAAATCGGCA GCAAGCGGCT GAAGGTCCAA 
CTTAAACGTT CCAAGGATGC GGCTAAGCCG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246002.1
CDS	362..2317
Translation	MKNPNIMECGNSLNNNNIGGNINGNGNGNGGVSEQRPLSGKVHAASPPPVVGNNNNNNSNLIVRYNKLSSGSSSASSSPPHHQQHSPPPSMHHQSHPNHPLQPSSMSTNMATNGHQLYPAITATYWLPPPNPAPYLVPERKLFVGMLNKKYTEADVRQLFTGHGTIEECTVLRDQAGQSKGCAFVTFATKQNAIGAIKALHQSQTMEGCSAPLVVKFADTQKEKDQKKMQQIHAFCGINTPSGATAGAATPTINAATALIAAPPSAGRTNPSMAAALAAVPQVQQAGSAATAPTTLVPLNSTTALSASLTPNLLATNAAHQGAAAAAAYLGADPAAAAHLQLYQQLHGYGLSPAHYLPGLNFHHPPENSAHHSQHSPGIGGASAASLSAAAATAASNPLGGAPPPTPTPTSSAGHAAGAGLLAAPSMSMQNLVALLATPTAHHQLSLHHSSSSSGHNNGNSSATSGLHNQRLTYAAAPPPPPISTTYNMPQLIGHTATPTQPGGSSQSLTFNALWPPNAADPYSSSLSGLTNGAAYGAASQPVTTSALQAAAAGVTGKQIEGPDGSNLFIYHLPQEFIDTDLASTFLPFGNVLSAKVFIDKQTNLSKCFGFVSYDNPHSANAAIQAMHGFQIGSKRLKVQLKRSKDAAKPY

Target ORF information:

RefSeq Version	NM_001259073.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bruno 2 (bru2), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259073.1

ORF Insert Sequence:

ATGAAAAACC CAAATATCAT GGAATGCGGC AATTCCTTAA ACAACAACAA CATTGGCGGC 
AATATCAACG GAAACGGAAA TGGAAATGGC GGAGTCAGCG AACAACGCCC CCTATCGGGC 
AAAGTCCATG CTGCATCTCC TCCGCCCGTC GTCGGCAACA ACAACAACAA CAATAGTAAT 
CTAATAGTCC GTTACAACAA ACTAAGCTCG GGCAGCTCCT CCGCGAGCTC ATCGCCGCCA 
CATCATCAGC AGCACTCGCC ACCACCGTCG ATGCACCACC AAAGCCATCC CAACCACCCA 
CTCCAGCCGT CCTCCATGTC CACAAACATG GCCACAAATG GCCATCAGTT GTATCCGGCG 
ATAACGGCAA CGTATTGGCT CCCGCCGCCG AATCCGGCGC CATATTTAGT TCCAGAACGT 
AAACTTTTCG TTGGCATGCT GAACAAAAAG TACACCGAGG CCGATGTGAG GCAGCTCTTC 
ACGGGCCACG GAACCATCGA GGAGTGCACA GTGCTCCGGG ATCAGGCTGG TCAGAGCAAG 
GGCTGCGCCT TCGTCACATT CGCCACTAAG CAGAACGCCA TTGGAGCTAT AAAGGCCCTC 
CATCAAAGTC AGACCATGGA AGGCTGTTCA GCTCCGCTGG TTGTGAAATT CGCCGACACG 
CAAAAGGAAA AAGACCAAAA GAAGATGCAG CAGATTCACG CATTCTGTGG CATCAATACG 
CCGAGCGGTG CGACAGCAGG TGCGGCCACG CCCACCATTA ATGCCGCAAC GGCTCTAATA 
GCTGCTCCGC CCTCGGCGGG ACGTACCAAT CCGTCGATGG CGGCCGCACT GGCGGCAGTG 
CCTCAGGTTC AGCAGGCTGG TTCTGCAGCA ACGGCACCAA CCACCCTCGT TCCCCTGAAT 
TCCACCACCG CCCTCAGTGC GTCCTTGACG CCCAATCTCC TAGCCACCAA TGCCGCCCAC 
CAGGGGGCGG CAGCTGCGGC CGCCTATTTG GGGGCAGATC CCGCTGCGGC GGCCCACCTT 
CAGCTGTACC AACAACTACA CGGATACGGA CTGAGTCCGG CACACTATCT GCCAGGACTA 
AATTTCCACC ACCCACCGGA AAACAGTGCC CACCACAGCC AGCACAGTCC CGGCATCGGC 
GGCGCATCAG CAGCATCTCT ATCGGCTGCA GCAGCCACAG CAGCATCTAA CCCACTTGGC 
GGCGCACCAC CACCCACACC CACACCCACA AGTTCCGCCG GGCATGCTGC TGGCGCTGGA 
CTGCTCGCCG CTCCATCCAT GTCTATGCAG AATCTGGTAG CCCTACTAGC CACGCCCACC 
GCCCACCACC AGCTGTCCCT CCATCACAGC AGCAGCAGCA GCGGCCACAA CAACGGCAAC 
AGCAGCGCCA CCAGCGGCCT CCACAACCAG CGGCTGACCT ACGCGGCCGC ACCACCACCA 
CCACCCATCT CGACCACCTA CAACATGCCG CAGCTAATCG GTCACACGGC CACGCCCACC 
CAGCCAGGGG GCAGCAGCCA ATCGCTGACC TTCAATGCGC TGTGGCCTCC AAATGCCGCC 
GACCCCTATT CATCCTCGCT GAGCGGACTG ACAAATGGAG CCGCCTACGG AGCCGCATCT 
CAACCTGTGA CCACCAGCGC CCTTCAAGCG GCAGCTGCCG GCGTGACTGG CAAGCAGATC 
GAGGGTCCCG ATGGCAGCAA TCTTTTCATT TATCACCTAC CTCAGGAGTT CATTGACACA 
GATCTCGCGT CGACGTTTCT TCCCTTCGGC AATGTCCTTT CGGCCAAGGT GTTCATCGAT 
AAGCAGACCA ACCTGTCGAA GTGTTTCGGC TTTGTGTCCT ACGATAATCC CCACTCGGCC 
AACGCCGCTA TCCAGGCGAT GCATGGCTTT CAAATCGGCA GCAAGCGGCT GAAGGTCCAA 
CTTAAACGTT CCAAGGATGC GGCTAAGCCG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bru2 ( NM_001259070.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245999.1 Drosophila melanogaster bruno 2 (bru2), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa15795
Clone ID Related Accession (Same CDS sequence)	NM_001259073.1 , NM_001259070.1
Accession Version	NM_001259070.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bruno 2, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAACC CAAATATCAT GGAATGCGGC AATTCCTTAA ACAACAACAA CATTGGCGGC 
AATATCAACG GAAACGGAAA TGGAAATGGC GGAGTCAGCG AACAACGCCC CCTATCGGGC 
AAAGTCCATG CTGCATCTCC TCCGCCCGTC GTCGGCAACA ACAACAACAA CAATAGTAAT 
CTAATAGTCC GTTACAACAA ACTAAGCTCG GGCAGCTCCT CCGCGAGCTC ATCGCCGCCA 
CATCATCAGC AGCACTCGCC ACCACCGTCG ATGCACCACC AAAGCCATCC CAACCACCCA 
CTCCAGCCGT CCTCCATGTC CACAAACATG GCCACAAATG GCCATCAGTT GTATCCGGCG 
ATAACGGCAA CGTATTGGCT CCCGCCGCCG AATCCGGCGC CATATTTAGT TCCAGAACGT 
AAACTTTTCG TTGGCATGCT GAACAAAAAG TACACCGAGG CCGATGTGAG GCAGCTCTTC 
ACGGGCCACG GAACCATCGA GGAGTGCACA GTGCTCCGGG ATCAGGCTGG TCAGAGCAAG 
GGCTGCGCCT TCGTCACATT CGCCACTAAG CAGAACGCCA TTGGAGCTAT AAAGGCCCTC 
CATCAAAGTC AGACCATGGA AGGCTGTTCA GCTCCGCTGG TTGTGAAATT CGCCGACACG 
CAAAAGGAAA AAGACCAAAA GAAGATGCAG CAGATTCACG CATTCTGTGG CATCAATACG 
CCGAGCGGTG CGACAGCAGG TGCGGCCACG CCCACCATTA ATGCCGCAAC GGCTCTAATA 
GCTGCTCCGC CCTCGGCGGG ACGTACCAAT CCGTCGATGG CGGCCGCACT GGCGGCAGTG 
CCTCAGGTTC AGCAGGCTGG TTCTGCAGCA ACGGCACCAA CCACCCTCGT TCCCCTGAAT 
TCCACCACCG CCCTCAGTGC GTCCTTGACG CCCAATCTCC TAGCCACCAA TGCCGCCCAC 
CAGGGGGCGG CAGCTGCGGC CGCCTATTTG GGGGCAGATC CCGCTGCGGC GGCCCACCTT 
CAGCTGTACC AACAACTACA CGGATACGGA CTGAGTCCGG CACACTATCT GCCAGGACTA 
AATTTCCACC ACCCACCGGA AAACAGTGCC CACCACAGCC AGCACAGTCC CGGCATCGGC 
GGCGCATCAG CAGCATCTCT ATCGGCTGCA GCAGCCACAG CAGCATCTAA CCCACTTGGC 
GGCGCACCAC CACCCACACC CACACCCACA AGTTCCGCCG GGCATGCTGC TGGCGCTGGA 
CTGCTCGCCG CTCCATCCAT GTCTATGCAG AATCTGGTAG CCCTACTAGC CACGCCCACC 
GCCCACCACC AGCTGTCCCT CCATCACAGC AGCAGCAGCA GCGGCCACAA CAACGGCAAC 
AGCAGCGCCA CCAGCGGCCT CCACAACCAG CGGCTGACCT ACGCGGCCGC ACCACCACCA 
CCACCCATCT CGACCACCTA CAACATGCCG CAGCTAATCG GTCACACGGC CACGCCCACC 
CAGCCAGGGG GCAGCAGCCA ATCGCTGACC TTCAATGCGC TGTGGCCTCC AAATGCCGCC 
GACCCCTATT CATCCTCGCT GAGCGGACTG ACAAATGGAG CCGCCTACGG AGCCGCATCT 
CAACCTGTGA CCACCAGCGC CCTTCAAGCG GCAGCTGCCG GCGTGACTGG CAAGCAGATC 
GAGGGTCCCG ATGGCAGCAA TCTTTTCATT TATCACCTAC CTCAGGAGTT CATTGACACA 
GATCTCGCGT CGACGTTTCT TCCCTTCGGC AATGTCCTTT CGGCCAAGGT GTTCATCGAT 
AAGCAGACCA ACCTGTCGAA GTGTTTCGGC TTTGTGTCCT ACGATAATCC CCACTCGGCC 
AACGCCGCTA TCCAGGCGAT GCATGGCTTT CAAATCGGCA GCAAGCGGCT GAAGGTCCAA 
CTTAAACGTT CCAAGGATGC GGCTAAGCCG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245999.1
CDS	271..2226
Translation	MKNPNIMECGNSLNNNNIGGNINGNGNGNGGVSEQRPLSGKVHAASPPPVVGNNNNNNSNLIVRYNKLSSGSSSASSSPPHHQQHSPPPSMHHQSHPNHPLQPSSMSTNMATNGHQLYPAITATYWLPPPNPAPYLVPERKLFVGMLNKKYTEADVRQLFTGHGTIEECTVLRDQAGQSKGCAFVTFATKQNAIGAIKALHQSQTMEGCSAPLVVKFADTQKEKDQKKMQQIHAFCGINTPSGATAGAATPTINAATALIAAPPSAGRTNPSMAAALAAVPQVQQAGSAATAPTTLVPLNSTTALSASLTPNLLATNAAHQGAAAAAAYLGADPAAAAHLQLYQQLHGYGLSPAHYLPGLNFHHPPENSAHHSQHSPGIGGASAASLSAAAATAASNPLGGAPPPTPTPTSSAGHAAGAGLLAAPSMSMQNLVALLATPTAHHQLSLHHSSSSSGHNNGNSSATSGLHNQRLTYAAAPPPPPISTTYNMPQLIGHTATPTQPGGSSQSLTFNALWPPNAADPYSSSLSGLTNGAAYGAASQPVTTSALQAAAAGVTGKQIEGPDGSNLFIYHLPQEFIDTDLASTFLPFGNVLSAKVFIDKQTNLSKCFGFVSYDNPHSANAAIQAMHGFQIGSKRLKVQLKRSKDAAKPY

Target ORF information:

RefSeq Version	NM_001259070.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bruno 2 (bru2), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259070.1

ORF Insert Sequence:

ATGAAAAACC CAAATATCAT GGAATGCGGC AATTCCTTAA ACAACAACAA CATTGGCGGC 
AATATCAACG GAAACGGAAA TGGAAATGGC GGAGTCAGCG AACAACGCCC CCTATCGGGC 
AAAGTCCATG CTGCATCTCC TCCGCCCGTC GTCGGCAACA ACAACAACAA CAATAGTAAT 
CTAATAGTCC GTTACAACAA ACTAAGCTCG GGCAGCTCCT CCGCGAGCTC ATCGCCGCCA 
CATCATCAGC AGCACTCGCC ACCACCGTCG ATGCACCACC AAAGCCATCC CAACCACCCA 
CTCCAGCCGT CCTCCATGTC CACAAACATG GCCACAAATG GCCATCAGTT GTATCCGGCG 
ATAACGGCAA CGTATTGGCT CCCGCCGCCG AATCCGGCGC CATATTTAGT TCCAGAACGT 
AAACTTTTCG TTGGCATGCT GAACAAAAAG TACACCGAGG CCGATGTGAG GCAGCTCTTC 
ACGGGCCACG GAACCATCGA GGAGTGCACA GTGCTCCGGG ATCAGGCTGG TCAGAGCAAG 
GGCTGCGCCT TCGTCACATT CGCCACTAAG CAGAACGCCA TTGGAGCTAT AAAGGCCCTC 
CATCAAAGTC AGACCATGGA AGGCTGTTCA GCTCCGCTGG TTGTGAAATT CGCCGACACG 
CAAAAGGAAA AAGACCAAAA GAAGATGCAG CAGATTCACG CATTCTGTGG CATCAATACG 
CCGAGCGGTG CGACAGCAGG TGCGGCCACG CCCACCATTA ATGCCGCAAC GGCTCTAATA 
GCTGCTCCGC CCTCGGCGGG ACGTACCAAT CCGTCGATGG CGGCCGCACT GGCGGCAGTG 
CCTCAGGTTC AGCAGGCTGG TTCTGCAGCA ACGGCACCAA CCACCCTCGT TCCCCTGAAT 
TCCACCACCG CCCTCAGTGC GTCCTTGACG CCCAATCTCC TAGCCACCAA TGCCGCCCAC 
CAGGGGGCGG CAGCTGCGGC CGCCTATTTG GGGGCAGATC CCGCTGCGGC GGCCCACCTT 
CAGCTGTACC AACAACTACA CGGATACGGA CTGAGTCCGG CACACTATCT GCCAGGACTA 
AATTTCCACC ACCCACCGGA AAACAGTGCC CACCACAGCC AGCACAGTCC CGGCATCGGC 
GGCGCATCAG CAGCATCTCT ATCGGCTGCA GCAGCCACAG CAGCATCTAA CCCACTTGGC 
GGCGCACCAC CACCCACACC CACACCCACA AGTTCCGCCG GGCATGCTGC TGGCGCTGGA 
CTGCTCGCCG CTCCATCCAT GTCTATGCAG AATCTGGTAG CCCTACTAGC CACGCCCACC 
GCCCACCACC AGCTGTCCCT CCATCACAGC AGCAGCAGCA GCGGCCACAA CAACGGCAAC 
AGCAGCGCCA CCAGCGGCCT CCACAACCAG CGGCTGACCT ACGCGGCCGC ACCACCACCA 
CCACCCATCT CGACCACCTA CAACATGCCG CAGCTAATCG GTCACACGGC CACGCCCACC 
CAGCCAGGGG GCAGCAGCCA ATCGCTGACC TTCAATGCGC TGTGGCCTCC AAATGCCGCC 
GACCCCTATT CATCCTCGCT GAGCGGACTG ACAAATGGAG CCGCCTACGG AGCCGCATCT 
CAACCTGTGA CCACCAGCGC CCTTCAAGCG GCAGCTGCCG GCGTGACTGG CAAGCAGATC 
GAGGGTCCCG ATGGCAGCAA TCTTTTCATT TATCACCTAC CTCAGGAGTT CATTGACACA 
GATCTCGCGT CGACGTTTCT TCCCTTCGGC AATGTCCTTT CGGCCAAGGT GTTCATCGAT 
AAGCAGACCA ACCTGTCGAA GTGTTTCGGC TTTGTGTCCT ACGATAATCC CCACTCGGCC 
AACGCCGCTA TCCAGGCGAT GCATGGCTTT CAAATCGGCA GCAAGCGGCT GAAGGTCCAA 
CTTAAACGTT CCAAGGATGC GGCTAAGCCG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	bru2
Entrez Gene ID	250811
Full Name	bruno 2
Synonyms	Bru-2,CG17008,CG31761,CG31763,CG43065,CG6319,Dm Bru-2,Dmel\CG43065,Dmel_CG31761,Dmel_CG31763,bru-2
General protein information	Preferred Names bruno 2 Names CG43065 gene product from transcript CG43065-RE CG43065-PA CG43065-PD CG43065-PE CG43065-PF CG43065-PG CG43065-PH CG43065-PI CG43065-PJ CG43065-PK CG43065-PL CG43065-PM bru2-PA bru2-PD bru2-PE bru2-PF bru2-PG bru2-PH bru2-PI bru2-PJ bru2-PK bru2-PL bru2-PM bruno-2 bruno2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 33E1-33E1

Homo sapiens (human)	CELF2	NP_006552.3
Canis lupus familiaris (dog)	CELF2	XP_005617210.1
Gallus gallus (chicken)	CELF2	NP_989591.1
Danio rerio (zebrafish)	celf2	NP_919382.1
Drosophila melanogaster (fruit fly)	bru-2	NP_001260410.1
Xenopus tropicalis (tropical clawed frog)	celf2	NP_001096417.1
Pan troglodytes (chimpanzee)	CELF2	XP_003312501.1
Caenorhabditis elegans (roundworm)	etr-1	NP_001122632.1
Mus musculus (house mouse)	Celf2	NP_001103701.1
Rattus norvegicus (Norway rat)	Celf2	NP_058893.2
Drosophila melanogaster (fruit fly)	aret	NP_723738.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

bru2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

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