bru2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following bru2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bru2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa15796 NM_001259072.1
Latest version!
Drosophila melanogaster bruno 2 (bru2), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OFa15386 NM_001042891.3
Latest version!
Drosophila melanogaster bruno 2 (bru2), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa15386 NM_001273482.1
Latest version!
Drosophila melanogaster bruno 2 (bru2), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OFa15387 NM_176025.3
Latest version!
Drosophila melanogaster bruno 2 (bru2), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa15387 NM_001273481.1
Latest version!
Drosophila melanogaster bruno 2 (bru2), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OFa15388 NM_135715.4
Latest version!
Drosophila melanogaster bruno 2 (bru2), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OFa15389 NM_001259071.2
Latest version!
Drosophila melanogaster bruno 2 (bru2), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OFa15389 NM_165007.3
Latest version!
Drosophila melanogaster bruno 2 (bru2), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OFa15794 NM_001259069.2
Latest version!
Drosophila melanogaster bruno 2 (bru2), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $258.30
$369.00
OFa15795 NM_001259070.1
Latest version!
Drosophila melanogaster bruno 2 (bru2), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OFa15795 NM_001259073.1
Latest version!
Drosophila melanogaster bruno 2 (bru2), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa15796
    Clone ID Related Accession (Same CDS sequence) NM_001259072.1
    Accession Version NM_001259072.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 2, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAACA AAAAGTACAC CGAGGCCGAT GTGAGGCAGC TCTTCACGGG CCACGGAACC 
    ATCGAGGAGT GCACAGTGCT CCGGGATCAG GCTGGTCAGA GCAAGGGCTG CGCCTTCGTC
    ACATTCGCCA CTAAGCAGAA CGCCATTGGA GCTATAAAGG CCCTCCATCA AAGTCAGACC
    ATGGAAGGCT GTTCAGCTCC GCTGGTTGTG AAATTCGCCG ACACGCAAAA GGAAAAAGAC
    CAAAAGAAGA TGCAGCAGAT TCACGCATTC TGTGGCATCA ATACGCCGAG CGGTGCGACA
    GCAGGTGCGG CCACGCCCAC CATTAATGCC GCAACGGCTC TAATAGCTGC TCCGCCCTCG
    GCGGGACGTA CCAATCCGTC GATGGCGGCC GCACTGGCGG CAGTGCCTCA GGTTCAGCAG
    GCTGGTTCTG CAGCAACGGC ACCAACCACC CTCGTTCCCC TGAATTCCAC CACCGCCCTC
    AGTGCGTCCT TGACGCCCAA TCTCCTAGCC ACCAATGCCG CCCACCAGGG GGCGGCAGCT
    GCGGCCGCCT ATTTGGGGGC AGATCCCGCT GCGGCGGCCC ACCTTCAGCT GTACCAACAA
    CTACACGGAT ACGGACTGAG TCCGGCACAC TATCTGCCAG GGCCTCCAAA TGCCGCCGAC
    CCCTATTCAT CCTCGCTGAG CGGACTGACA AATGGAGCCG CCTACGGAGC CGCATCTCAA
    CCTGTGACCA CCAGCGCCCT TCAAGCGGCA GCTGCCGGCG TGACTGGCAA GCAGATCGAG
    GGTCCCGATG GCAGCAATCT TTTCATTTAT CACCTACCTC AGGAGTTCAT TGACACAGAT
    CTCGCGTCGA CGTTTCTTCC CTTCGGCAAT GTCCTTTCGG CCAAGGTGTT CATCGATAAG
    CAGACCAACC TGTCGAAGTG TTTCGGCTTT GTGTCCTACG ATAATCCCCA CTCGGCCAAC
    GCCGCTATCC AGGCGATGCA TGGCTTTCAA ATCGGCAGCA AGCGGCTGAA GGTCCAACTT
    AAACGTTCCA AGGATGCGGC TAAGCCGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246001.1
    CDS268..1320
    Translation

    Target ORF information:

    RefSeq Version NM_001259072.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 2 (bru2), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259072.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCTGAACA AAAAGTACAC CGAGGCCGAT GTGAGGCAGC TCTTCACGGG CCACGGAACC 
    ATCGAGGAGT GCACAGTGCT CCGGGATCAG GCTGGTCAGA GCAAGGGCTG CGCCTTCGTC
    ACATTCGCCA CTAAGCAGAA CGCCATTGGA GCTATAAAGG CCCTCCATCA AAGTCAGACC
    ATGGAAGGCT GTTCAGCTCC GCTGGTTGTG AAATTCGCCG ACACGCAAAA GGAAAAAGAC
    CAAAAGAAGA TGCAGCAGAT TCACGCATTC TGTGGCATCA ATACGCCGAG CGGTGCGACA
    GCAGGTGCGG CCACGCCCAC CATTAATGCC GCAACGGCTC TAATAGCTGC TCCGCCCTCG
    GCGGGACGTA CCAATCCGTC GATGGCGGCC GCACTGGCGG CAGTGCCTCA GGTTCAGCAG
    GCTGGTTCTG CAGCAACGGC ACCAACCACC CTCGTTCCCC TGAATTCCAC CACCGCCCTC
    AGTGCGTCCT TGACGCCCAA TCTCCTAGCC ACCAATGCCG CCCACCAGGG GGCGGCAGCT
    GCGGCCGCCT ATTTGGGGGC AGATCCCGCT GCGGCGGCCC ACCTTCAGCT GTACCAACAA
    CTACACGGAT ACGGACTGAG TCCGGCACAC TATCTGCCAG GGCCTCCAAA TGCCGCCGAC
    CCCTATTCAT CCTCGCTGAG CGGACTGACA AATGGAGCCG CCTACGGAGC CGCATCTCAA
    CCTGTGACCA CCAGCGCCCT TCAAGCGGCA GCTGCCGGCG TGACTGGCAA GCAGATCGAG
    GGTCCCGATG GCAGCAATCT TTTCATTTAT CACCTACCTC AGGAGTTCAT TGACACAGAT
    CTCGCGTCGA CGTTTCTTCC CTTCGGCAAT GTCCTTTCGG CCAAGGTGTT CATCGATAAG
    CAGACCAACC TGTCGAAGTG TTTCGGCTTT GTGTCCTACG ATAATCCCCA CTCGGCCAAC
    GCCGCTATCC AGGCGATGCA TGGCTTTCAA ATCGGCAGCA AGCGGCTGAA GGTCCAACTT
    AAACGTTCCA AGGATGCGGC TAAGCCGTAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15386
    Clone ID Related Accession (Same CDS sequence) NM_001042891.3 , NM_001273482.1
    Accession Version NM_001042891.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 2, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001042891.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GGACTAAATT TCCACCACCC ACCGGAAAAC AGTGCCCACC ACAGCCAGCA CAGTCCCGGC
    ATCGGCGGCG CATCAGCAGC ATCTCTATCG GCTGCAGCAG CCACAGCAGC ATCTAACCCA
    CTTGGCGGCG CACCACCACC CACACCCACA CCCACAAGTT CCGCCGGGCA TGCTGCTGGC
    GCTGGACTGC TCGCCGCTCC ATCCATGTCT ATGCAGAATC TGGTAGCCCT ACTAGCCACG
    CCCACCGCCC ACCACCAGCT GTCCCTCCAT CACAGCAGCA GCAGCAGCGG CCACAACAAC
    GGCAACAGCA GCGCCACCAG CGGCCTCCAC AACCAGCGGC TGACCTACGC GGCCGCACCA
    CCACCACCAC CCATCTCGAC CACCTACAAC ATGCCGCAGC TAATCGGTCA CACGGCCACG
    CCCACCCAGC CAGGGGGCAG CAGCCAATCG CTGACCTTCA ATGCGCTGTG GCCTCCAAAT
    GCCGCCGACC CCTATTCATC CTCGCTGAGC GGACTGACAA ATGGAGCCGC CTACGGAGCC
    GCATCTCAAC CTGTGACCAC CAGCGCCCTT CAAGCGGCAG CTGCCGGCGT GACTGGCAAG
    CAGATCGAGG GTCCCGATGG CAGCAATCTT TTCATTTATC ACCTACCTCA GGAGTTCATT
    GACACAGATC TCGCGTCGAC GTTTCTTCCC TTCGGCAATG TCCTTTCGGC CAAGGTGTTC
    ATCGATAAGC AGACCAACCT GTCGAAGTGT TTCGGCTTTG TGTCCTACGA TAATCCCCAC
    TCGGCCAACG CCGCTATCCA GGCGATGCAT GGCTTTCAAA TCGGCAGCAA GCGGCTGAAG
    GTCCAACTTA AACGTTCCAA GGATGCGGCT AAGCCGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001036356.1
    CDS625..3306
    Translation

    Target ORF information:

    RefSeq Version NM_001042891.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 2 (bru2), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042891.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GGACTAAATT TCCACCACCC ACCGGAAAAC AGTGCCCACC ACAGCCAGCA CAGTCCCGGC
    ATCGGCGGCG CATCAGCAGC ATCTCTATCG GCTGCAGCAG CCACAGCAGC ATCTAACCCA
    CTTGGCGGCG CACCACCACC CACACCCACA CCCACAAGTT CCGCCGGGCA TGCTGCTGGC
    GCTGGACTGC TCGCCGCTCC ATCCATGTCT ATGCAGAATC TGGTAGCCCT ACTAGCCACG
    CCCACCGCCC ACCACCAGCT GTCCCTCCAT CACAGCAGCA GCAGCAGCGG CCACAACAAC
    GGCAACAGCA GCGCCACCAG CGGCCTCCAC AACCAGCGGC TGACCTACGC GGCCGCACCA
    CCACCACCAC CCATCTCGAC CACCTACAAC ATGCCGCAGC TAATCGGTCA CACGGCCACG
    CCCACCCAGC CAGGGGGCAG CAGCCAATCG CTGACCTTCA ATGCGCTGTG GCCTCCAAAT
    GCCGCCGACC CCTATTCATC CTCGCTGAGC GGACTGACAA ATGGAGCCGC CTACGGAGCC
    GCATCTCAAC CTGTGACCAC CAGCGCCCTT CAAGCGGCAG CTGCCGGCGT GACTGGCAAG
    CAGATCGAGG GTCCCGATGG CAGCAATCTT TTCATTTATC ACCTACCTCA GGAGTTCATT
    GACACAGATC TCGCGTCGAC GTTTCTTCCC TTCGGCAATG TCCTTTCGGC CAAGGTGTTC
    ATCGATAAGC AGACCAACCT GTCGAAGTGT TTCGGCTTTG TGTCCTACGA TAATCCCCAC
    TCGGCCAACG CCGCTATCCA GGCGATGCAT GGCTTTCAAA TCGGCAGCAA GCGGCTGAAG
    GTCCAACTTA AACGTTCCAA GGATGCGGCT AAGCCGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15386
    Clone ID Related Accession (Same CDS sequence) NM_001042891.3 , NM_001273482.1
    Accession Version NM_001273482.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 2, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GGACTAAATT TCCACCACCC ACCGGAAAAC AGTGCCCACC ACAGCCAGCA CAGTCCCGGC
    ATCGGCGGCG CATCAGCAGC ATCTCTATCG GCTGCAGCAG CCACAGCAGC ATCTAACCCA
    CTTGGCGGCG CACCACCACC CACACCCACA CCCACAAGTT CCGCCGGGCA TGCTGCTGGC
    GCTGGACTGC TCGCCGCTCC ATCCATGTCT ATGCAGAATC TGGTAGCCCT ACTAGCCACG
    CCCACCGCCC ACCACCAGCT GTCCCTCCAT CACAGCAGCA GCAGCAGCGG CCACAACAAC
    GGCAACAGCA GCGCCACCAG CGGCCTCCAC AACCAGCGGC TGACCTACGC GGCCGCACCA
    CCACCACCAC CCATCTCGAC CACCTACAAC ATGCCGCAGC TAATCGGTCA CACGGCCACG
    CCCACCCAGC CAGGGGGCAG CAGCCAATCG CTGACCTTCA ATGCGCTGTG GCCTCCAAAT
    GCCGCCGACC CCTATTCATC CTCGCTGAGC GGACTGACAA ATGGAGCCGC CTACGGAGCC
    GCATCTCAAC CTGTGACCAC CAGCGCCCTT CAAGCGGCAG CTGCCGGCGT GACTGGCAAG
    CAGATCGAGG GTCCCGATGG CAGCAATCTT TTCATTTATC ACCTACCTCA GGAGTTCATT
    GACACAGATC TCGCGTCGAC GTTTCTTCCC TTCGGCAATG TCCTTTCGGC CAAGGTGTTC
    ATCGATAAGC AGACCAACCT GTCGAAGTGT TTCGGCTTTG TGTCCTACGA TAATCCCCAC
    TCGGCCAACG CCGCTATCCA GGCGATGCAT GGCTTTCAAA TCGGCAGCAA GCGGCTGAAG
    GTCCAACTTA AACGTTCCAA GGATGCGGCT AAGCCGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260411.1
    CDS532..3213
    Translation

    Target ORF information:

    RefSeq Version NM_001273482.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 2 (bru2), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GGACTAAATT TCCACCACCC ACCGGAAAAC AGTGCCCACC ACAGCCAGCA CAGTCCCGGC
    ATCGGCGGCG CATCAGCAGC ATCTCTATCG GCTGCAGCAG CCACAGCAGC ATCTAACCCA
    CTTGGCGGCG CACCACCACC CACACCCACA CCCACAAGTT CCGCCGGGCA TGCTGCTGGC
    GCTGGACTGC TCGCCGCTCC ATCCATGTCT ATGCAGAATC TGGTAGCCCT ACTAGCCACG
    CCCACCGCCC ACCACCAGCT GTCCCTCCAT CACAGCAGCA GCAGCAGCGG CCACAACAAC
    GGCAACAGCA GCGCCACCAG CGGCCTCCAC AACCAGCGGC TGACCTACGC GGCCGCACCA
    CCACCACCAC CCATCTCGAC CACCTACAAC ATGCCGCAGC TAATCGGTCA CACGGCCACG
    CCCACCCAGC CAGGGGGCAG CAGCCAATCG CTGACCTTCA ATGCGCTGTG GCCTCCAAAT
    GCCGCCGACC CCTATTCATC CTCGCTGAGC GGACTGACAA ATGGAGCCGC CTACGGAGCC
    GCATCTCAAC CTGTGACCAC CAGCGCCCTT CAAGCGGCAG CTGCCGGCGT GACTGGCAAG
    CAGATCGAGG GTCCCGATGG CAGCAATCTT TTCATTTATC ACCTACCTCA GGAGTTCATT
    GACACAGATC TCGCGTCGAC GTTTCTTCCC TTCGGCAATG TCCTTTCGGC CAAGGTGTTC
    ATCGATAAGC AGACCAACCT GTCGAAGTGT TTCGGCTTTG TGTCCTACGA TAATCCCCAC
    TCGGCCAACG CCGCTATCCA GGCGATGCAT GGCTTTCAAA TCGGCAGCAA GCGGCTGAAG
    GTCCAACTTA AACGTTCCAA GGATGCGGCT AAGCCGTACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15387
    Clone ID Related Accession (Same CDS sequence) NM_176025.3 , NM_001273481.1
    Accession Version NM_176025.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2214bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 2, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_176025.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GGGCCTCCAA ATGCCGCCGA CCCCTATTCA TCCTCGCTGA GCGGACTGAC AAATGGAGCC
    GCCTACGGAG CCGCATCTCA ACCTGTGACC ACCAGCGCCC TTCAAGCGGC AGCTGCCGGC
    GTGACTGGCA AGCAGATCGA GGGTCCCGAT GGCAGCAATC TTTTCATTTA TCACCTACCT
    CAGGAGTTCA TTGACACAGA TCTCGCGTCG ACGTTTCTTC CCTTCGGCAA TGTCCTTTCG
    GCCAAGGTGT TCATCGATAA GCAGACCAAC CTGTCGAAGT GTTTCGGCTT TGTGTCCTAC
    GATAATCCCC ACTCGGCCAA CGCCGCTATC CAGGCGATGC ATGGCTTTCA AATCGGCAGC
    AAGCGGCTGA AGGTCCAACT TAAACGTTCC AAGGATGCGG CTAAGCCGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788039.1
    CDS625..2838
    Translation

    Target ORF information:

    RefSeq Version NM_176025.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 2 (bru2), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176025.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GGGCCTCCAA ATGCCGCCGA CCCCTATTCA TCCTCGCTGA GCGGACTGAC AAATGGAGCC
    GCCTACGGAG CCGCATCTCA ACCTGTGACC ACCAGCGCCC TTCAAGCGGC AGCTGCCGGC
    GTGACTGGCA AGCAGATCGA GGGTCCCGAT GGCAGCAATC TTTTCATTTA TCACCTACCT
    CAGGAGTTCA TTGACACAGA TCTCGCGTCG ACGTTTCTTC CCTTCGGCAA TGTCCTTTCG
    GCCAAGGTGT TCATCGATAA GCAGACCAAC CTGTCGAAGT GTTTCGGCTT TGTGTCCTAC
    GATAATCCCC ACTCGGCCAA CGCCGCTATC CAGGCGATGC ATGGCTTTCA AATCGGCAGC
    AAGCGGCTGA AGGTCCAACT TAAACGTTCC AAGGATGCGG CTAAGCCGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15387
    Clone ID Related Accession (Same CDS sequence) NM_176025.3 , NM_001273481.1
    Accession Version NM_001273481.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2214bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 2, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GGGCCTCCAA ATGCCGCCGA CCCCTATTCA TCCTCGCTGA GCGGACTGAC AAATGGAGCC
    GCCTACGGAG CCGCATCTCA ACCTGTGACC ACCAGCGCCC TTCAAGCGGC AGCTGCCGGC
    GTGACTGGCA AGCAGATCGA GGGTCCCGAT GGCAGCAATC TTTTCATTTA TCACCTACCT
    CAGGAGTTCA TTGACACAGA TCTCGCGTCG ACGTTTCTTC CCTTCGGCAA TGTCCTTTCG
    GCCAAGGTGT TCATCGATAA GCAGACCAAC CTGTCGAAGT GTTTCGGCTT TGTGTCCTAC
    GATAATCCCC ACTCGGCCAA CGCCGCTATC CAGGCGATGC ATGGCTTTCA AATCGGCAGC
    AAGCGGCTGA AGGTCCAACT TAAACGTTCC AAGGATGCGG CTAAGCCGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260410.1
    CDS908..3121
    Translation

    Target ORF information:

    RefSeq Version NM_001273481.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 2 (bru2), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273481.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GGGCCTCCAA ATGCCGCCGA CCCCTATTCA TCCTCGCTGA GCGGACTGAC AAATGGAGCC
    GCCTACGGAG CCGCATCTCA ACCTGTGACC ACCAGCGCCC TTCAAGCGGC AGCTGCCGGC
    GTGACTGGCA AGCAGATCGA GGGTCCCGAT GGCAGCAATC TTTTCATTTA TCACCTACCT
    CAGGAGTTCA TTGACACAGA TCTCGCGTCG ACGTTTCTTC CCTTCGGCAA TGTCCTTTCG
    GCCAAGGTGT TCATCGATAA GCAGACCAAC CTGTCGAAGT GTTTCGGCTT TGTGTCCTAC
    GATAATCCCC ACTCGGCCAA CGCCGCTATC CAGGCGATGC ATGGCTTTCA AATCGGCAGC
    AAGCGGCTGA AGGTCCAACT TAAACGTTCC AAGGATGCGG CTAAGCCGTA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15388
    Clone ID Related Accession (Same CDS sequence) NM_135715.4
    Accession Version NM_135715.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1899bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 2, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135715.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GATGAGTCAA AAAAAAGTAC AAAGGAAAAG AAAAAAACGT CTTCGTCTAC CGTTTTCCCT
    GCTTTTCTTT GCCCATTTTC CGACTTGCAA TTTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_609559.1
    CDS532..2430
    Translation

    Target ORF information:

    RefSeq Version NM_135715.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 2 (bru2), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_135715.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GATGAGTCAA AAAAAAGTAC AAAGGAAAAG AAAAAAACGT CTTCGTCTAC CGTTTTCCCT
    GCTTTTCTTT GCCCATTTTC CGACTTGCAA TTTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15389
    Clone ID Related Accession (Same CDS sequence) NM_001259071.2 , NM_165007.3
    Accession Version NM_001259071.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1995bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 2, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259071.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GTGCCCACCA CAGCCAGCAC AGTCCCGGCA TCGGCGGCGC ATCAGCAGCA TCTCTATCGG
    CTGCAGCAGC CACAGCAGCA TCTAACCCAC TTGGCGGCGC ACCACCACCC ACACCCACAC
    CCACAAGTTC CGCCGGGCAT GCTGCTGGCG CTGGACTGCT CGCCGCTCCA TCCATGTCTA
    TGCAGAATCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246000.1
    CDS625..2619
    Translation

    Target ORF information:

    RefSeq Version NM_001259071.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 2 (bru2), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259071.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GTGCCCACCA CAGCCAGCAC AGTCCCGGCA TCGGCGGCGC ATCAGCAGCA TCTCTATCGG
    CTGCAGCAGC CACAGCAGCA TCTAACCCAC TTGGCGGCGC ACCACCACCC ACACCCACAC
    CCACAAGTTC CGCCGGGCAT GCTGCTGGCG CTGGACTGCT CGCCGCTCCA TCCATGTCTA
    TGCAGAATCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15389
    Clone ID Related Accession (Same CDS sequence) NM_001259071.2 , NM_165007.3
    Accession Version NM_165007.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1995bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 2, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165007.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GTGCCCACCA CAGCCAGCAC AGTCCCGGCA TCGGCGGCGC ATCAGCAGCA TCTCTATCGG
    CTGCAGCAGC CACAGCAGCA TCTAACCCAC TTGGCGGCGC ACCACCACCC ACACCCACAC
    CCACAAGTTC CGCCGGGCAT GCTGCTGGCG CTGGACTGCT CGCCGCTCCA TCCATGTCTA
    TGCAGAATCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_723742.2
    CDS532..2526
    Translation

    Target ORF information:

    RefSeq Version NM_165007.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 2 (bru2), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165007.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGG GTGCTGTTTT
    GTCACCTACT ATACAAGAAA GGCCGCCCTG CGCGCCCAGG ACGCGTTGCA CAACATCAAG
    ACCTTGGATG GGATGCACCA TCCCATTCAG ATGAAGCCGG CGGATAGTGA GAACAGAAAT
    GAACGTAAAC TTTTCGTTGG CATGCTGAAC AAAAAGTACA CCGAGGCCGA TGTGAGGCAG
    CTCTTCACGG GCCACGGAAC CATCGAGGAG TGCACAGTGC TCCGGGATCA GGCTGGTCAG
    AGCAAGGGCT GCGCCTTCGT CACATTCGCC ACTAAGCAGA ACGCCATTGG AGCTATAAAG
    GCCCTCCATC AAAGTCAGAC CATGGAAGGC TGTTCAGCTC CGCTGGTTGT GAAATTCGCC
    GACACGCAAA AGGAAAAAGA CCAAAAGAAG ATGCAGCAGA TTCACGCATT CTGTGGCATC
    AATACGCCGA GCGGTGCGAC AGCAGGTGCG GCCACGCCCA CCATTAATGC CGCAACGGCT
    CTAATAGCTG CTCCGCCCTC GGCGGGACGT ACCAATCCGT CGATGGCGGC CGCACTGGCG
    GCAGTGCCTC AGGTTCAGCA GGCTGGTTCT GCAGCAACGG CACCAACCAC CCTCGTTCCC
    CTGAATTCCA CCACCGCCCT CAGTGCGTCC TTGACGCCCA ATCTCCTAGC CACCAATGCC
    GCCCACCAGG GGGCGGCAGC TGCGGCCGCC TATTTGGGGG CAGATCCCGC TGCGGCGGCC
    CACCTTCAGC TGTACCAACA ACTACACGGA TACGGACTGA GTCCGGCACA CTATCTGCCA
    GTGCCCACCA CAGCCAGCAC AGTCCCGGCA TCGGCGGCGC ATCAGCAGCA TCTCTATCGG
    CTGCAGCAGC CACAGCAGCA TCTAACCCAC TTGGCGGCGC ACCACCACCC ACACCCACAC
    CCACAAGTTC CGCCGGGCAT GCTGCTGGCG CTGGACTGCT CGCCGCTCCA TCCATGTCTA
    TGCAGAATCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15794
    Clone ID Related Accession (Same CDS sequence) NM_001259069.2
    Accession Version NM_001259069.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 2, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259069.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGA TGCACCATCC
    CATTCAGATG AAGCCGGCGG ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245998.1
    CDS532..1575
    Translation

    Target ORF information:

    RefSeq Version NM_001259069.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 2 (bru2), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259069.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGATGTTGC AATCCTTGAG TCTGCACGCG GATAAACTAA GCGGCAATTC ACCAGCAGCA 
    GCAGCAGCAA CTGGAGCAGC AGCAGCAACA TCAGAGGCAA CAGCAGTAGC ATCCGCAGCA
    GCAGCAACAA CAATGCTTGC AACGGCACCT GTTGCCCATG TTGCTAATGG CAACAACAGC
    AATTCGAGTG CCGGCAGCAG CAGCAACAGC AACAGCAGCA GCAACAACCA CAGCCTCTAC
    AGCAATAACA ATTTAAATAC AGGCAACAAT AGCCAGCAGC AGCAGCAGCA GCAGCAGCAA
    CATCCGCAAA TACTGCCGGT CAAGTACGAG TATTTAGAGA ATCTAACGAG CTCGGGCGCA
    ACTTCAGGTG CCATTGCAGC GACAACAACT TTCACGGGAG CTGCGAAAAG TTTTCATCCC
    TATTTGCGGC CCACCGGCAG TGGCAGCAAC AGCAACAACA GCATCGCAGC ATTATCTACA
    ACAACGGCAG CAACAGCGGC TAAAATGTCG ACAACATTGT TTGAGACACT CTTGCACAAC
    CAAATAAATT CCAGCCCAAT AGCACTGCCA GCAGCAACGG CAATCGCAGC AATATCTGCA
    GCAGCAACAG CAGCAACAGC AACACCAGGC ACCGCAGCAA CCGCAGCAGC TGCCGCTGCA
    GCAGCACCGT CAACGCAGCT GAATTCCAGC ATTAATTCCA GAACTTCATT AGGCGACCAG
    GGTGGTGCAA TTGTTGTTGT TACCCCCGCA CCACCCGTTG CTGCACCACC AACTGGCGGC
    CCAAATCGCA ACCAAAGTCC CACGCGTGAC TGCTCGGTCA AAATGGAGCT CATGGAATCG
    ACCAGCCCGG ATTCGATCAA GGATCAGCCG GATGCGGACA ATATCAAGAT GTTCGTGGGC
    CAAATACCGA AGACCTGGGA CGAGACGAGG CTACGCCAAA TGTTCGAGCA GTTCGGACCC
    GTTCACACAC TGAATGTGCT ACGGGACAAG GTCACATCGA TTAGCCGAGA TGCACCATCC
    CATTCAGATG AAGCCGGCGG ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15795
    Clone ID Related Accession (Same CDS sequence) NM_001259073.1 , NM_001259070.1
    Accession Version NM_001259073.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 2, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAAAAACC CAAATATCAT GGAATGCGGC AATTCCTTAA ACAACAACAA CATTGGCGGC 
    AATATCAACG GAAACGGAAA TGGAAATGGC GGAGTCAGCG AACAACGCCC CCTATCGGGC
    AAAGTCCATG CTGCATCTCC TCCGCCCGTC GTCGGCAACA ACAACAACAA CAATAGTAAT
    CTAATAGTCC GTTACAACAA ACTAAGCTCG GGCAGCTCCT CCGCGAGCTC ATCGCCGCCA
    CATCATCAGC AGCACTCGCC ACCACCGTCG ATGCACCACC AAAGCCATCC CAACCACCCA
    CTCCAGCCGT CCTCCATGTC CACAAACATG GCCACAAATG GCCATCAGTT GTATCCGGCG
    ATAACGGCAA CGTATTGGCT CCCGCCGCCG AATCCGGCGC CATATTTAGT TCCAGAACGT
    AAACTTTTCG TTGGCATGCT GAACAAAAAG TACACCGAGG CCGATGTGAG GCAGCTCTTC
    ACGGGCCACG GAACCATCGA GGAGTGCACA GTGCTCCGGG ATCAGGCTGG TCAGAGCAAG
    GGCTGCGCCT TCGTCACATT CGCCACTAAG CAGAACGCCA TTGGAGCTAT AAAGGCCCTC
    CATCAAAGTC AGACCATGGA AGGCTGTTCA GCTCCGCTGG TTGTGAAATT CGCCGACACG
    CAAAAGGAAA AAGACCAAAA GAAGATGCAG CAGATTCACG CATTCTGTGG CATCAATACG
    CCGAGCGGTG CGACAGCAGG TGCGGCCACG CCCACCATTA ATGCCGCAAC GGCTCTAATA
    GCTGCTCCGC CCTCGGCGGG ACGTACCAAT CCGTCGATGG CGGCCGCACT GGCGGCAGTG
    CCTCAGGTTC AGCAGGCTGG TTCTGCAGCA ACGGCACCAA CCACCCTCGT TCCCCTGAAT
    TCCACCACCG CCCTCAGTGC GTCCTTGACG CCCAATCTCC TAGCCACCAA TGCCGCCCAC
    CAGGGGGCGG CAGCTGCGGC CGCCTATTTG GGGGCAGATC CCGCTGCGGC GGCCCACCTT
    CAGCTGTACC AACAACTACA CGGATACGGA CTGAGTCCGG CACACTATCT GCCAGGACTA
    AATTTCCACC ACCCACCGGA AAACAGTGCC CACCACAGCC AGCACAGTCC CGGCATCGGC
    GGCGCATCAG CAGCATCTCT ATCGGCTGCA GCAGCCACAG CAGCATCTAA CCCACTTGGC
    GGCGCACCAC CACCCACACC CACACCCACA AGTTCCGCCG GGCATGCTGC TGGCGCTGGA
    CTGCTCGCCG CTCCATCCAT GTCTATGCAG AATCTGGTAG CCCTACTAGC CACGCCCACC
    GCCCACCACC AGCTGTCCCT CCATCACAGC AGCAGCAGCA GCGGCCACAA CAACGGCAAC
    AGCAGCGCCA CCAGCGGCCT CCACAACCAG CGGCTGACCT ACGCGGCCGC ACCACCACCA
    CCACCCATCT CGACCACCTA CAACATGCCG CAGCTAATCG GTCACACGGC CACGCCCACC
    CAGCCAGGGG GCAGCAGCCA ATCGCTGACC TTCAATGCGC TGTGGCCTCC AAATGCCGCC
    GACCCCTATT CATCCTCGCT GAGCGGACTG ACAAATGGAG CCGCCTACGG AGCCGCATCT
    CAACCTGTGA CCACCAGCGC CCTTCAAGCG GCAGCTGCCG GCGTGACTGG CAAGCAGATC
    GAGGGTCCCG ATGGCAGCAA TCTTTTCATT TATCACCTAC CTCAGGAGTT CATTGACACA
    GATCTCGCGT CGACGTTTCT TCCCTTCGGC AATGTCCTTT CGGCCAAGGT GTTCATCGAT
    AAGCAGACCA ACCTGTCGAA GTGTTTCGGC TTTGTGTCCT ACGATAATCC CCACTCGGCC
    AACGCCGCTA TCCAGGCGAT GCATGGCTTT CAAATCGGCA GCAAGCGGCT GAAGGTCCAA
    CTTAAACGTT CCAAGGATGC GGCTAAGCCG TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246002.1
    CDS362..2317
    Translation

    Target ORF information:

    RefSeq Version NM_001259073.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 2 (bru2), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259073.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAAAAACC CAAATATCAT GGAATGCGGC AATTCCTTAA ACAACAACAA CATTGGCGGC 
    AATATCAACG GAAACGGAAA TGGAAATGGC GGAGTCAGCG AACAACGCCC CCTATCGGGC
    AAAGTCCATG CTGCATCTCC TCCGCCCGTC GTCGGCAACA ACAACAACAA CAATAGTAAT
    CTAATAGTCC GTTACAACAA ACTAAGCTCG GGCAGCTCCT CCGCGAGCTC ATCGCCGCCA
    CATCATCAGC AGCACTCGCC ACCACCGTCG ATGCACCACC AAAGCCATCC CAACCACCCA
    CTCCAGCCGT CCTCCATGTC CACAAACATG GCCACAAATG GCCATCAGTT GTATCCGGCG
    ATAACGGCAA CGTATTGGCT CCCGCCGCCG AATCCGGCGC CATATTTAGT TCCAGAACGT
    AAACTTTTCG TTGGCATGCT GAACAAAAAG TACACCGAGG CCGATGTGAG GCAGCTCTTC
    ACGGGCCACG GAACCATCGA GGAGTGCACA GTGCTCCGGG ATCAGGCTGG TCAGAGCAAG
    GGCTGCGCCT TCGTCACATT CGCCACTAAG CAGAACGCCA TTGGAGCTAT AAAGGCCCTC
    CATCAAAGTC AGACCATGGA AGGCTGTTCA GCTCCGCTGG TTGTGAAATT CGCCGACACG
    CAAAAGGAAA AAGACCAAAA GAAGATGCAG CAGATTCACG CATTCTGTGG CATCAATACG
    CCGAGCGGTG CGACAGCAGG TGCGGCCACG CCCACCATTA ATGCCGCAAC GGCTCTAATA
    GCTGCTCCGC CCTCGGCGGG ACGTACCAAT CCGTCGATGG CGGCCGCACT GGCGGCAGTG
    CCTCAGGTTC AGCAGGCTGG TTCTGCAGCA ACGGCACCAA CCACCCTCGT TCCCCTGAAT
    TCCACCACCG CCCTCAGTGC GTCCTTGACG CCCAATCTCC TAGCCACCAA TGCCGCCCAC
    CAGGGGGCGG CAGCTGCGGC CGCCTATTTG GGGGCAGATC CCGCTGCGGC GGCCCACCTT
    CAGCTGTACC AACAACTACA CGGATACGGA CTGAGTCCGG CACACTATCT GCCAGGACTA
    AATTTCCACC ACCCACCGGA AAACAGTGCC CACCACAGCC AGCACAGTCC CGGCATCGGC
    GGCGCATCAG CAGCATCTCT ATCGGCTGCA GCAGCCACAG CAGCATCTAA CCCACTTGGC
    GGCGCACCAC CACCCACACC CACACCCACA AGTTCCGCCG GGCATGCTGC TGGCGCTGGA
    CTGCTCGCCG CTCCATCCAT GTCTATGCAG AATCTGGTAG CCCTACTAGC CACGCCCACC
    GCCCACCACC AGCTGTCCCT CCATCACAGC AGCAGCAGCA GCGGCCACAA CAACGGCAAC
    AGCAGCGCCA CCAGCGGCCT CCACAACCAG CGGCTGACCT ACGCGGCCGC ACCACCACCA
    CCACCCATCT CGACCACCTA CAACATGCCG CAGCTAATCG GTCACACGGC CACGCCCACC
    CAGCCAGGGG GCAGCAGCCA ATCGCTGACC TTCAATGCGC TGTGGCCTCC AAATGCCGCC
    GACCCCTATT CATCCTCGCT GAGCGGACTG ACAAATGGAG CCGCCTACGG AGCCGCATCT
    CAACCTGTGA CCACCAGCGC CCTTCAAGCG GCAGCTGCCG GCGTGACTGG CAAGCAGATC
    GAGGGTCCCG ATGGCAGCAA TCTTTTCATT TATCACCTAC CTCAGGAGTT CATTGACACA
    GATCTCGCGT CGACGTTTCT TCCCTTCGGC AATGTCCTTT CGGCCAAGGT GTTCATCGAT
    AAGCAGACCA ACCTGTCGAA GTGTTTCGGC TTTGTGTCCT ACGATAATCC CCACTCGGCC
    AACGCCGCTA TCCAGGCGAT GCATGGCTTT CAAATCGGCA GCAAGCGGCT GAAGGTCCAA
    CTTAAACGTT CCAAGGATGC GGCTAAGCCG TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa15795
    Clone ID Related Accession (Same CDS sequence) NM_001259073.1 , NM_001259070.1
    Accession Version NM_001259070.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product bruno 2, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAAAAACC CAAATATCAT GGAATGCGGC AATTCCTTAA ACAACAACAA CATTGGCGGC 
    AATATCAACG GAAACGGAAA TGGAAATGGC GGAGTCAGCG AACAACGCCC CCTATCGGGC
    AAAGTCCATG CTGCATCTCC TCCGCCCGTC GTCGGCAACA ACAACAACAA CAATAGTAAT
    CTAATAGTCC GTTACAACAA ACTAAGCTCG GGCAGCTCCT CCGCGAGCTC ATCGCCGCCA
    CATCATCAGC AGCACTCGCC ACCACCGTCG ATGCACCACC AAAGCCATCC CAACCACCCA
    CTCCAGCCGT CCTCCATGTC CACAAACATG GCCACAAATG GCCATCAGTT GTATCCGGCG
    ATAACGGCAA CGTATTGGCT CCCGCCGCCG AATCCGGCGC CATATTTAGT TCCAGAACGT
    AAACTTTTCG TTGGCATGCT GAACAAAAAG TACACCGAGG CCGATGTGAG GCAGCTCTTC
    ACGGGCCACG GAACCATCGA GGAGTGCACA GTGCTCCGGG ATCAGGCTGG TCAGAGCAAG
    GGCTGCGCCT TCGTCACATT CGCCACTAAG CAGAACGCCA TTGGAGCTAT AAAGGCCCTC
    CATCAAAGTC AGACCATGGA AGGCTGTTCA GCTCCGCTGG TTGTGAAATT CGCCGACACG
    CAAAAGGAAA AAGACCAAAA GAAGATGCAG CAGATTCACG CATTCTGTGG CATCAATACG
    CCGAGCGGTG CGACAGCAGG TGCGGCCACG CCCACCATTA ATGCCGCAAC GGCTCTAATA
    GCTGCTCCGC CCTCGGCGGG ACGTACCAAT CCGTCGATGG CGGCCGCACT GGCGGCAGTG
    CCTCAGGTTC AGCAGGCTGG TTCTGCAGCA ACGGCACCAA CCACCCTCGT TCCCCTGAAT
    TCCACCACCG CCCTCAGTGC GTCCTTGACG CCCAATCTCC TAGCCACCAA TGCCGCCCAC
    CAGGGGGCGG CAGCTGCGGC CGCCTATTTG GGGGCAGATC CCGCTGCGGC GGCCCACCTT
    CAGCTGTACC AACAACTACA CGGATACGGA CTGAGTCCGG CACACTATCT GCCAGGACTA
    AATTTCCACC ACCCACCGGA AAACAGTGCC CACCACAGCC AGCACAGTCC CGGCATCGGC
    GGCGCATCAG CAGCATCTCT ATCGGCTGCA GCAGCCACAG CAGCATCTAA CCCACTTGGC
    GGCGCACCAC CACCCACACC CACACCCACA AGTTCCGCCG GGCATGCTGC TGGCGCTGGA
    CTGCTCGCCG CTCCATCCAT GTCTATGCAG AATCTGGTAG CCCTACTAGC CACGCCCACC
    GCCCACCACC AGCTGTCCCT CCATCACAGC AGCAGCAGCA GCGGCCACAA CAACGGCAAC
    AGCAGCGCCA CCAGCGGCCT CCACAACCAG CGGCTGACCT ACGCGGCCGC ACCACCACCA
    CCACCCATCT CGACCACCTA CAACATGCCG CAGCTAATCG GTCACACGGC CACGCCCACC
    CAGCCAGGGG GCAGCAGCCA ATCGCTGACC TTCAATGCGC TGTGGCCTCC AAATGCCGCC
    GACCCCTATT CATCCTCGCT GAGCGGACTG ACAAATGGAG CCGCCTACGG AGCCGCATCT
    CAACCTGTGA CCACCAGCGC CCTTCAAGCG GCAGCTGCCG GCGTGACTGG CAAGCAGATC
    GAGGGTCCCG ATGGCAGCAA TCTTTTCATT TATCACCTAC CTCAGGAGTT CATTGACACA
    GATCTCGCGT CGACGTTTCT TCCCTTCGGC AATGTCCTTT CGGCCAAGGT GTTCATCGAT
    AAGCAGACCA ACCTGTCGAA GTGTTTCGGC TTTGTGTCCT ACGATAATCC CCACTCGGCC
    AACGCCGCTA TCCAGGCGAT GCATGGCTTT CAAATCGGCA GCAAGCGGCT GAAGGTCCAA
    CTTAAACGTT CCAAGGATGC GGCTAAGCCG TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245999.1
    CDS271..2226
    Translation

    Target ORF information:

    RefSeq Version NM_001259070.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster bruno 2 (bru2), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259070.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAAAAACC CAAATATCAT GGAATGCGGC AATTCCTTAA ACAACAACAA CATTGGCGGC 
    AATATCAACG GAAACGGAAA TGGAAATGGC GGAGTCAGCG AACAACGCCC CCTATCGGGC
    AAAGTCCATG CTGCATCTCC TCCGCCCGTC GTCGGCAACA ACAACAACAA CAATAGTAAT
    CTAATAGTCC GTTACAACAA ACTAAGCTCG GGCAGCTCCT CCGCGAGCTC ATCGCCGCCA
    CATCATCAGC AGCACTCGCC ACCACCGTCG ATGCACCACC AAAGCCATCC CAACCACCCA
    CTCCAGCCGT CCTCCATGTC CACAAACATG GCCACAAATG GCCATCAGTT GTATCCGGCG
    ATAACGGCAA CGTATTGGCT CCCGCCGCCG AATCCGGCGC CATATTTAGT TCCAGAACGT
    AAACTTTTCG TTGGCATGCT GAACAAAAAG TACACCGAGG CCGATGTGAG GCAGCTCTTC
    ACGGGCCACG GAACCATCGA GGAGTGCACA GTGCTCCGGG ATCAGGCTGG TCAGAGCAAG
    GGCTGCGCCT TCGTCACATT CGCCACTAAG CAGAACGCCA TTGGAGCTAT AAAGGCCCTC
    CATCAAAGTC AGACCATGGA AGGCTGTTCA GCTCCGCTGG TTGTGAAATT CGCCGACACG
    CAAAAGGAAA AAGACCAAAA GAAGATGCAG CAGATTCACG CATTCTGTGG CATCAATACG
    CCGAGCGGTG CGACAGCAGG TGCGGCCACG CCCACCATTA ATGCCGCAAC GGCTCTAATA
    GCTGCTCCGC CCTCGGCGGG ACGTACCAAT CCGTCGATGG CGGCCGCACT GGCGGCAGTG
    CCTCAGGTTC AGCAGGCTGG TTCTGCAGCA ACGGCACCAA CCACCCTCGT TCCCCTGAAT
    TCCACCACCG CCCTCAGTGC GTCCTTGACG CCCAATCTCC TAGCCACCAA TGCCGCCCAC
    CAGGGGGCGG CAGCTGCGGC CGCCTATTTG GGGGCAGATC CCGCTGCGGC GGCCCACCTT
    CAGCTGTACC AACAACTACA CGGATACGGA CTGAGTCCGG CACACTATCT GCCAGGACTA
    AATTTCCACC ACCCACCGGA AAACAGTGCC CACCACAGCC AGCACAGTCC CGGCATCGGC
    GGCGCATCAG CAGCATCTCT ATCGGCTGCA GCAGCCACAG CAGCATCTAA CCCACTTGGC
    GGCGCACCAC CACCCACACC CACACCCACA AGTTCCGCCG GGCATGCTGC TGGCGCTGGA
    CTGCTCGCCG CTCCATCCAT GTCTATGCAG AATCTGGTAG CCCTACTAGC CACGCCCACC
    GCCCACCACC AGCTGTCCCT CCATCACAGC AGCAGCAGCA GCGGCCACAA CAACGGCAAC
    AGCAGCGCCA CCAGCGGCCT CCACAACCAG CGGCTGACCT ACGCGGCCGC ACCACCACCA
    CCACCCATCT CGACCACCTA CAACATGCCG CAGCTAATCG GTCACACGGC CACGCCCACC
    CAGCCAGGGG GCAGCAGCCA ATCGCTGACC TTCAATGCGC TGTGGCCTCC AAATGCCGCC
    GACCCCTATT CATCCTCGCT GAGCGGACTG ACAAATGGAG CCGCCTACGG AGCCGCATCT
    CAACCTGTGA CCACCAGCGC CCTTCAAGCG GCAGCTGCCG GCGTGACTGG CAAGCAGATC
    GAGGGTCCCG ATGGCAGCAA TCTTTTCATT TATCACCTAC CTCAGGAGTT CATTGACACA
    GATCTCGCGT CGACGTTTCT TCCCTTCGGC AATGTCCTTT CGGCCAAGGT GTTCATCGAT
    AAGCAGACCA ACCTGTCGAA GTGTTTCGGC TTTGTGTCCT ACGATAATCC CCACTCGGCC
    AACGCCGCTA TCCAGGCGAT GCATGGCTTT CAAATCGGCA GCAAGCGGCT GAAGGTCCAA
    CTTAAACGTT CCAAGGATGC GGCTAAGCCG TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.