Dsp1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dsp1
Entrez Gene ID	117294
Full Name	Dorsal switch protein 1
Synonyms	CG12223,DSP1,Dmel\CG12223,Hmg14B,Hmg14D,Ssrp2,dsp1,hmg,ssrp2
General protein information	Preferred Names Dorsal switch protein 1 Names CG12223 gene product from transcript CG12223-RE CG12223-PA CG12223-PB CG12223-PC CG12223-PD CG12223-PE CG12223-PF CG12223-PG Dsp1-PA Dsp1-PB Dsp1-PC Dsp1-PD Dsp1-PE Dsp1-PF Dsp1-PG Hmg14B dorsal switch protein 1 dorsal switch protein-1 structure specific recognition protein 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14C1-14B15

mRNA and Protein(s)

mRNA	Protein	Name
NM_167502.3	NP_727959.2	dorsal switch protein 1, isoform D
NM_167504.2	NP_727961.1	dorsal switch protein 1, isoform B
NM_167503.3	NP_727960.1	dorsal switch protein 1, isoform A
NM_206762.3	NP_996485.1	dorsal switch protein 1, isoform E
NM_001144731.3	NP_001138203.1	dorsal switch protein 1, isoform F
NM_001298393.1	NP_001285322.1	dorsal switch protein 1, isoform G
NM_080715.4	NP_542446.2	dorsal switch protein 1, isoform C

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Hmgb2	NP_032278.1
Danio rerio (zebrafish)	hmgb2a	NP_001032501.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000005	XP_311155.4
Caenorhabditis elegans (roundworm)	hmg-1.2	NP_001022600.1
Bos taurus (cattle)	HMGB2	NP_001032705.1
Gallus gallus (chicken)	HMGB2	NP_990817.1
Drosophila melanogaster (fruit fly)	Dsp1	NP_001138203.1
Canis lupus familiaris (dog)	HMGB2	XP_543194.2
Homo sapiens (human)	HMGB2	NP_002120.1
Pan troglodytes (chimpanzee)	HMGB2	XP_517538.3
	LOC697057	XP_001085665.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rm62, a DEAD-box RNA helicase, complexes with DSP1 in Drosophila embryos
Title: Rm62, a DEAD-box RNA helicase, complexes with DSP1 in Drosophila embryos.

While Dorsal seems to be nuclearly localized in ventral nuclei, it is actively shuttling into and out of all nuclei, including nuclei on the dorsal side.
Title: Nucleocytoplasmic shuttling mediates the dynamic maintenance of nuclear Dorsal levels during Drosophila embryogenesis.

Molecular modeling was used to compare the tertiary structure of Dsp1 with two other Drosophila proteins.
Title: Functional conservation of Rel binding sites in drosophilid genomes.

DSP1 and corto proteins co-localize at 91 sites on polytene chromosomes and co-immunoprecipitate in embryos.
Title: Corto and DSP1 interact and bind to a maintenance element of the Scr Hox gene: understanding the role of Enhancers of trithorax and Polycomb.

affects gene expression regulation in Drosophila.
Title: [Recruitment of Drosophila Polycomb group proteins to chromatin by DSP1].

The following Dsp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dsp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02474	NM_206762.3 Latest version!	Drosophila melanogaster dorsal switch protein 1, transcript variant E (Dsp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa02475	NM_080715.4 Latest version!	Drosophila melanogaster dorsal switch protein 1, transcript variant C (Dsp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa02475	NM_167504.2 Latest version!	Drosophila melanogaster dorsal switch protein 1, transcript variant B (Dsp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa02475	NM_167503.3 Latest version!	Drosophila melanogaster dorsal switch protein 1, transcript variant A (Dsp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa02476	NM_167502.3 Latest version!	Drosophila melanogaster dorsal switch protein 1, transcript variant D (Dsp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa02476	NM_001298393.1 Latest version!	Drosophila melanogaster dorsal switch protein 1, transcript variant G (Dsp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa02477	NM_001144731.3 Latest version!	Drosophila melanogaster dorsal switch protein 1, transcript variant F (Dsp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dsp1 ( NM_206762.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996485.1 Drosophila melanogaster dorsal switch protein 1, transcript variant E (Dsp1), mRNA.

Price & Availability↑

CloneID	OFa02474
Clone ID Related Accession (Same CDS sequence)	NM_206762.3
Accession Version	NM_206762.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dorsal switch protein 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_206762.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACACT TTCATCAAAT ACAGCAAACA ATTCAACACT ATCAGCAGCA ATTGGCTGCT 
CAGCAACAAC AACAAGTACA ACAGCAACAG TTGCAACAAC ACCAGGTGGT CGTCCAGCAG 
AATCAGCAGC AGGCGCACCA GAACAGCTCC AATACCACAG CCGGCGTGGG CACCCAACAG 
CTGTTCACAT ACAAAATGGC CAGCAGTTTT CCGAATCCGG CCACGACGAT GGCCCAAGTG 
GTGGCCACAT CGAACGCAGC CGGCACCACG GGCTATGATT ATCGTCTAAA TATGGCACAG 
GCAGCCGCAG CAGCAGCCGT ACCCGGCTCC CAGTGGTGGT ATTCGGCCGC CAATCAGGGA 
CAGGTCGATG CCAATACAGC GGCGCAACTG CAGCACCAGC AGCAGCAACA GCAACAGCAA 
CAACAGCAGC AGCAGCAACA GCACCAGCAG CAACAACAGA TGCAACAGCA GCAGCAGCAA 
CAGAATGTCA TCAATTCGGC GAGCCCCATG AGCAGAGTCA AGGCCGATGC CAAGCCCAGA 
GGCCGAATGA CCGCCTACGC ATACTTTGTA CAAACTTGCA GAGAGGAGCA CAAAAAGAAA 
CATCCCGATG AGACGGTGAT ATTCGCCGAA TTCTCGCGGA AGTGCGCCGA ACGGTGGAAG 
ACAATGGTGG ACAAGGAGAA GAAGCGCTTC CACGAAATGG CCGAGAAGGA CAAGCAGCGA 
TATGAGGCGG AAATGCAGAA CTATGTGCCG CCCAAGGGAG CGGTTGTGGG ACGCGGCAAG 
AAGAGGAAAC AGATCAAGGA CCCCAATGCT CCAAAACGTT CATTGTCTGC GTTCTTCTGG 
TTCTGCAATG ACGAGAGAAA TAAGGTGAAG GCACTTAATC CCGAGTTCGG AGTGGGCGAC 
ATCGCCAAAG AGCTGGGACG AAAGTGGTCC GATGTGGATC CCGAGGTCAA GCAGAAGTAC 
GAGTCGATGG CCGAGCGGGA CAAGGCTCGA TATGAACGCG AGATGACCGA GTACAAGACG 
AGCGGGAAAA TAGCCATGTC CGCACCGTCG ATGCAGGCGT CGATGCAGGC GCAGGCACAG 
AAAGCCGCGT TACTTGCCGC CGCTGCCCAG CAGCAGCACC AGCAGCTGGA AGAGCAGCAC 
GACGACGACG ACGGCGATGG AGATGATGAC GAGAACCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996485.1
CDS	242..1423
Translation	MEHFHQIQQTIQHYQQQLAAQQQQQVQQQQLQQHQVVVQQNQQQAHQNSSNTTAGVGTQQLFTYKMASSFPNPATTMAQVVATSNAAGTTGYDYRLNMAQAAAAAAVPGSQWWYSAANQGQVDANTAAQLQHQQQQQQQQQQQQQQQHQQQQQMQQQQQQQNVINSASPMSRVKADAKPRGRMTAYAYFVQTCREEHKKKHPDETVIFAEFSRKCAERWKTMVDKEKKRFHEMAEKDKQRYEAEMQNYVPPKGAVVGRGKKRKQIKDPNAPKRSLSAFFWFCNDERNKVKALNPEFGVGDIAKELGRKWSDVDPEVKQKYESMAERDKARYEREMTEYKTSGKIAMSAPSMQASMQAQAQKAALLAAAAQQQHQQLEEQHDDDDGDGDDDENQ

Target ORF information:

RefSeq Version	NM_206762.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dorsal switch protein 1, transcript variant E (Dsp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206762.3

ORF Insert Sequence:

ATGGAACACT TTCATCAAAT ACAGCAAACA ATTCAACACT ATCAGCAGCA ATTGGCTGCT 
CAGCAACAAC AACAAGTACA ACAGCAACAG TTGCAACAAC ACCAGGTGGT CGTCCAGCAG 
AATCAGCAGC AGGCGCACCA GAACAGCTCC AATACCACAG CCGGCGTGGG CACCCAACAG 
CTGTTCACAT ACAAAATGGC CAGCAGTTTT CCGAATCCGG CCACGACGAT GGCCCAAGTG 
GTGGCCACAT CGAACGCAGC CGGCACCACG GGCTATGATT ATCGTCTAAA TATGGCACAG 
GCAGCCGCAG CAGCAGCCGT ACCCGGCTCC CAGTGGTGGT ATTCGGCCGC CAATCAGGGA 
CAGGTCGATG CCAATACAGC GGCGCAACTG CAGCACCAGC AGCAGCAACA GCAACAGCAA 
CAACAGCAGC AGCAGCAACA GCACCAGCAG CAACAACAGA TGCAACAGCA GCAGCAGCAA 
CAGAATGTCA TCAATTCGGC GAGCCCCATG AGCAGAGTCA AGGCCGATGC CAAGCCCAGA 
GGCCGAATGA CCGCCTACGC ATACTTTGTA CAAACTTGCA GAGAGGAGCA CAAAAAGAAA 
CATCCCGATG AGACGGTGAT ATTCGCCGAA TTCTCGCGGA AGTGCGCCGA ACGGTGGAAG 
ACAATGGTGG ACAAGGAGAA GAAGCGCTTC CACGAAATGG CCGAGAAGGA CAAGCAGCGA 
TATGAGGCGG AAATGCAGAA CTATGTGCCG CCCAAGGGAG CGGTTGTGGG ACGCGGCAAG 
AAGAGGAAAC AGATCAAGGA CCCCAATGCT CCAAAACGTT CATTGTCTGC GTTCTTCTGG 
TTCTGCAATG ACGAGAGAAA TAAGGTGAAG GCACTTAATC CCGAGTTCGG AGTGGGCGAC 
ATCGCCAAAG AGCTGGGACG AAAGTGGTCC GATGTGGATC CCGAGGTCAA GCAGAAGTAC 
GAGTCGATGG CCGAGCGGGA CAAGGCTCGA TATGAACGCG AGATGACCGA GTACAAGACG 
AGCGGGAAAA TAGCCATGTC CGCACCGTCG ATGCAGGCGT CGATGCAGGC GCAGGCACAG 
AAAGCCGCGT TACTTGCCGC CGCTGCCCAG CAGCAGCACC AGCAGCTGGA AGAGCAGCAC 
GACGACGACG ACGGCGATGG AGATGATGAC GAGAACCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsp1 ( NM_167504.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727961.1 Drosophila melanogaster dorsal switch protein 1, transcript variant B (Dsp1), mRNA.

Price & Availability↑

CloneID	OFa02475
Clone ID Related Accession (Same CDS sequence)	NM_167504.2 , NM_167503.3 , NM_080715.4
Accession Version	NM_167504.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dorsal switch protein 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_167504.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACACT TTCATCAAAT ACAGCAATTG GCTGCTCAGC AACAACAACA AGTACAACAG 
CAACAGTTGC AACAACACCA GGTGGTCGTC CAGCAGAATC AGCAGCAGGC GCACCAGAAC 
AGCTCCAATA CCACAGCCGG CGTGGGCACC CAACAGCTGT TCACATACAA AATGGCCAGC 
AGTTTTCCGA ATCCGGCCAC GACGATGGCC CAAGTGGTGG CCACATCGAA CGCAGCCGGC 
ACCACGGGCT ATGATTATCG TCTAAATATG GCACAGGCAG CCGCAGCAGC AGCCGTACCC 
GGCTCCCAGT GGTGGTATTC GGCCGCCAAT CAGGGACAGG TCGATGCCAA TACAGCGGCG 
CAACTGCAGC ACCAGCAGCA GCAACAGCAA CAGCAACAAC AGCAGCAGCA GCAACAGCAC 
CAGCAGCAAC AACAGATGCA ACAGCAGCAG CAGCAACAGA ATGTCATCAA TTCGGCGAGC 
CCCATGAGCA GAGTCAAGGC CGATGCCAAG CCCAGAGGCC GAATGACCGC CTACGCATAC 
TTTGTACAAA CTTGCAGAGA GGAGCACAAA AAGAAACATC CCGATGAGAC GGTGATATTC 
GCCGAATTCT CGCGGAAGTG CGCCGAACGG TGGAAGACAA TGGTGGACAA GGAGAAGAAG 
CGCTTCCACG AAATGGCCGA GAAGGACAAG CAGCGATATG AGGCGGAAAT GCAGAACTAT 
GTGCCGCCCA AGGGAGCGGT TGTGGGACGC GGCAAGAAGA GGAAACAGAT CAAGGACCCC 
AATGCTCCAA AACGTTCATT GTCTGCGTTC TTCTGGTTCT GCAATGACGA GAGAAATAAG 
GTGAAGGCAC TTAATCCCGA GTTCGGAGTG GGCGACATCG CCAAAGAGCT GGGACGAAAG 
TGGTCCGATG TGGATCCCGA GGTCAAGCAG AAGTACGAGT CGATGGCCGA GCGGGACAAG 
GCTCGATATG AACGCGAGAT GACCGAGTAC AAGACGAGCG GGAAAATAGC CATGTCCGCA 
CCGTCGATGC AGGCGTCGAT GCAGGCGCAG GCACAGAAAG CCGCGTTACT TGCCGCCGCT 
GCCCAGCAGC AGCACCAGCA GCTGGAAGAG CAGCACGACG ACGACGACGG CGATGGAGAT 
GATGACGAGA ACCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727961.1
CDS	122..1279
Translation	MEHFHQIQQLAAQQQQQVQQQQLQQHQVVVQQNQQQAHQNSSNTTAGVGTQQLFTYKMASSFPNPATTMAQVVATSNAAGTTGYDYRLNMAQAAAAAAVPGSQWWYSAANQGQVDANTAAQLQHQQQQQQQQQQQQQQQHQQQQQMQQQQQQQNVINSASPMSRVKADAKPRGRMTAYAYFVQTCREEHKKKHPDETVIFAEFSRKCAERWKTMVDKEKKRFHEMAEKDKQRYEAEMQNYVPPKGAVVGRGKKRKQIKDPNAPKRSLSAFFWFCNDERNKVKALNPEFGVGDIAKELGRKWSDVDPEVKQKYESMAERDKARYEREMTEYKTSGKIAMSAPSMQASMQAQAQKAALLAAAAQQQHQQLEEQHDDDDGDGDDDENQ

Target ORF information:

RefSeq Version	NM_167504.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dorsal switch protein 1, transcript variant B (Dsp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167504.2

ORF Insert Sequence:

ATGGAACACT TTCATCAAAT ACAGCAATTG GCTGCTCAGC AACAACAACA AGTACAACAG 
CAACAGTTGC AACAACACCA GGTGGTCGTC CAGCAGAATC AGCAGCAGGC GCACCAGAAC 
AGCTCCAATA CCACAGCCGG CGTGGGCACC CAACAGCTGT TCACATACAA AATGGCCAGC 
AGTTTTCCGA ATCCGGCCAC GACGATGGCC CAAGTGGTGG CCACATCGAA CGCAGCCGGC 
ACCACGGGCT ATGATTATCG TCTAAATATG GCACAGGCAG CCGCAGCAGC AGCCGTACCC 
GGCTCCCAGT GGTGGTATTC GGCCGCCAAT CAGGGACAGG TCGATGCCAA TACAGCGGCG 
CAACTGCAGC ACCAGCAGCA GCAACAGCAA CAGCAACAAC AGCAGCAGCA GCAACAGCAC 
CAGCAGCAAC AACAGATGCA ACAGCAGCAG CAGCAACAGA ATGTCATCAA TTCGGCGAGC 
CCCATGAGCA GAGTCAAGGC CGATGCCAAG CCCAGAGGCC GAATGACCGC CTACGCATAC 
TTTGTACAAA CTTGCAGAGA GGAGCACAAA AAGAAACATC CCGATGAGAC GGTGATATTC 
GCCGAATTCT CGCGGAAGTG CGCCGAACGG TGGAAGACAA TGGTGGACAA GGAGAAGAAG 
CGCTTCCACG AAATGGCCGA GAAGGACAAG CAGCGATATG AGGCGGAAAT GCAGAACTAT 
GTGCCGCCCA AGGGAGCGGT TGTGGGACGC GGCAAGAAGA GGAAACAGAT CAAGGACCCC 
AATGCTCCAA AACGTTCATT GTCTGCGTTC TTCTGGTTCT GCAATGACGA GAGAAATAAG 
GTGAAGGCAC TTAATCCCGA GTTCGGAGTG GGCGACATCG CCAAAGAGCT GGGACGAAAG 
TGGTCCGATG TGGATCCCGA GGTCAAGCAG AAGTACGAGT CGATGGCCGA GCGGGACAAG 
GCTCGATATG AACGCGAGAT GACCGAGTAC AAGACGAGCG GGAAAATAGC CATGTCCGCA 
CCGTCGATGC AGGCGTCGAT GCAGGCGCAG GCACAGAAAG CCGCGTTACT TGCCGCCGCT 
GCCCAGCAGC AGCACCAGCA GCTGGAAGAG CAGCACGACG ACGACGACGG CGATGGAGAT 
GATGACGAGA ACCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsp1 ( NM_167503.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727960.1 Drosophila melanogaster dorsal switch protein 1, transcript variant A (Dsp1), mRNA.

Price & Availability↑

CloneID	OFa02475
Clone ID Related Accession (Same CDS sequence)	NM_167504.2 , NM_167503.3 , NM_080715.4
Accession Version	NM_167503.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dorsal switch protein 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_167503.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACACT TTCATCAAAT ACAGCAATTG GCTGCTCAGC AACAACAACA AGTACAACAG 
CAACAGTTGC AACAACACCA GGTGGTCGTC CAGCAGAATC AGCAGCAGGC GCACCAGAAC 
AGCTCCAATA CCACAGCCGG CGTGGGCACC CAACAGCTGT TCACATACAA AATGGCCAGC 
AGTTTTCCGA ATCCGGCCAC GACGATGGCC CAAGTGGTGG CCACATCGAA CGCAGCCGGC 
ACCACGGGCT ATGATTATCG TCTAAATATG GCACAGGCAG CCGCAGCAGC AGCCGTACCC 
GGCTCCCAGT GGTGGTATTC GGCCGCCAAT CAGGGACAGG TCGATGCCAA TACAGCGGCG 
CAACTGCAGC ACCAGCAGCA GCAACAGCAA CAGCAACAAC AGCAGCAGCA GCAACAGCAC 
CAGCAGCAAC AACAGATGCA ACAGCAGCAG CAGCAACAGA ATGTCATCAA TTCGGCGAGC 
CCCATGAGCA GAGTCAAGGC CGATGCCAAG CCCAGAGGCC GAATGACCGC CTACGCATAC 
TTTGTACAAA CTTGCAGAGA GGAGCACAAA AAGAAACATC CCGATGAGAC GGTGATATTC 
GCCGAATTCT CGCGGAAGTG CGCCGAACGG TGGAAGACAA TGGTGGACAA GGAGAAGAAG 
CGCTTCCACG AAATGGCCGA GAAGGACAAG CAGCGATATG AGGCGGAAAT GCAGAACTAT 
GTGCCGCCCA AGGGAGCGGT TGTGGGACGC GGCAAGAAGA GGAAACAGAT CAAGGACCCC 
AATGCTCCAA AACGTTCATT GTCTGCGTTC TTCTGGTTCT GCAATGACGA GAGAAATAAG 
GTGAAGGCAC TTAATCCCGA GTTCGGAGTG GGCGACATCG CCAAAGAGCT GGGACGAAAG 
TGGTCCGATG TGGATCCCGA GGTCAAGCAG AAGTACGAGT CGATGGCCGA GCGGGACAAG 
GCTCGATATG AACGCGAGAT GACCGAGTAC AAGACGAGCG GGAAAATAGC CATGTCCGCA 
CCGTCGATGC AGGCGTCGAT GCAGGCGCAG GCACAGAAAG CCGCGTTACT TGCCGCCGCT 
GCCCAGCAGC AGCACCAGCA GCTGGAAGAG CAGCACGACG ACGACGACGG CGATGGAGAT 
GATGACGAGA ACCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727960.1
CDS	197..1354
Translation	MEHFHQIQQLAAQQQQQVQQQQLQQHQVVVQQNQQQAHQNSSNTTAGVGTQQLFTYKMASSFPNPATTMAQVVATSNAAGTTGYDYRLNMAQAAAAAAVPGSQWWYSAANQGQVDANTAAQLQHQQQQQQQQQQQQQQQHQQQQQMQQQQQQQNVINSASPMSRVKADAKPRGRMTAYAYFVQTCREEHKKKHPDETVIFAEFSRKCAERWKTMVDKEKKRFHEMAEKDKQRYEAEMQNYVPPKGAVVGRGKKRKQIKDPNAPKRSLSAFFWFCNDERNKVKALNPEFGVGDIAKELGRKWSDVDPEVKQKYESMAERDKARYEREMTEYKTSGKIAMSAPSMQASMQAQAQKAALLAAAAQQQHQQLEEQHDDDDGDGDDDENQ

Target ORF information:

RefSeq Version	NM_167503.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dorsal switch protein 1, transcript variant A (Dsp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167503.3

ORF Insert Sequence:

ATGGAACACT TTCATCAAAT ACAGCAATTG GCTGCTCAGC AACAACAACA AGTACAACAG 
CAACAGTTGC AACAACACCA GGTGGTCGTC CAGCAGAATC AGCAGCAGGC GCACCAGAAC 
AGCTCCAATA CCACAGCCGG CGTGGGCACC CAACAGCTGT TCACATACAA AATGGCCAGC 
AGTTTTCCGA ATCCGGCCAC GACGATGGCC CAAGTGGTGG CCACATCGAA CGCAGCCGGC 
ACCACGGGCT ATGATTATCG TCTAAATATG GCACAGGCAG CCGCAGCAGC AGCCGTACCC 
GGCTCCCAGT GGTGGTATTC GGCCGCCAAT CAGGGACAGG TCGATGCCAA TACAGCGGCG 
CAACTGCAGC ACCAGCAGCA GCAACAGCAA CAGCAACAAC AGCAGCAGCA GCAACAGCAC 
CAGCAGCAAC AACAGATGCA ACAGCAGCAG CAGCAACAGA ATGTCATCAA TTCGGCGAGC 
CCCATGAGCA GAGTCAAGGC CGATGCCAAG CCCAGAGGCC GAATGACCGC CTACGCATAC 
TTTGTACAAA CTTGCAGAGA GGAGCACAAA AAGAAACATC CCGATGAGAC GGTGATATTC 
GCCGAATTCT CGCGGAAGTG CGCCGAACGG TGGAAGACAA TGGTGGACAA GGAGAAGAAG 
CGCTTCCACG AAATGGCCGA GAAGGACAAG CAGCGATATG AGGCGGAAAT GCAGAACTAT 
GTGCCGCCCA AGGGAGCGGT TGTGGGACGC GGCAAGAAGA GGAAACAGAT CAAGGACCCC 
AATGCTCCAA AACGTTCATT GTCTGCGTTC TTCTGGTTCT GCAATGACGA GAGAAATAAG 
GTGAAGGCAC TTAATCCCGA GTTCGGAGTG GGCGACATCG CCAAAGAGCT GGGACGAAAG 
TGGTCCGATG TGGATCCCGA GGTCAAGCAG AAGTACGAGT CGATGGCCGA GCGGGACAAG 
GCTCGATATG AACGCGAGAT GACCGAGTAC AAGACGAGCG GGAAAATAGC CATGTCCGCA 
CCGTCGATGC AGGCGTCGAT GCAGGCGCAG GCACAGAAAG CCGCGTTACT TGCCGCCGCT 
GCCCAGCAGC AGCACCAGCA GCTGGAAGAG CAGCACGACG ACGACGACGG CGATGGAGAT 
GATGACGAGA ACCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsp1 ( NM_080715.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_542446.2 Drosophila melanogaster dorsal switch protein 1, transcript variant C (Dsp1), mRNA.

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CloneID	OFa02475
Clone ID Related Accession (Same CDS sequence)	NM_167504.2 , NM_167503.3 , NM_080715.4
Accession Version	NM_080715.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dorsal switch protein 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_080715.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACACT TTCATCAAAT ACAGCAATTG GCTGCTCAGC AACAACAACA AGTACAACAG 
CAACAGTTGC AACAACACCA GGTGGTCGTC CAGCAGAATC AGCAGCAGGC GCACCAGAAC 
AGCTCCAATA CCACAGCCGG CGTGGGCACC CAACAGCTGT TCACATACAA AATGGCCAGC 
AGTTTTCCGA ATCCGGCCAC GACGATGGCC CAAGTGGTGG CCACATCGAA CGCAGCCGGC 
ACCACGGGCT ATGATTATCG TCTAAATATG GCACAGGCAG CCGCAGCAGC AGCCGTACCC 
GGCTCCCAGT GGTGGTATTC GGCCGCCAAT CAGGGACAGG TCGATGCCAA TACAGCGGCG 
CAACTGCAGC ACCAGCAGCA GCAACAGCAA CAGCAACAAC AGCAGCAGCA GCAACAGCAC 
CAGCAGCAAC AACAGATGCA ACAGCAGCAG CAGCAACAGA ATGTCATCAA TTCGGCGAGC 
CCCATGAGCA GAGTCAAGGC CGATGCCAAG CCCAGAGGCC GAATGACCGC CTACGCATAC 
TTTGTACAAA CTTGCAGAGA GGAGCACAAA AAGAAACATC CCGATGAGAC GGTGATATTC 
GCCGAATTCT CGCGGAAGTG CGCCGAACGG TGGAAGACAA TGGTGGACAA GGAGAAGAAG 
CGCTTCCACG AAATGGCCGA GAAGGACAAG CAGCGATATG AGGCGGAAAT GCAGAACTAT 
GTGCCGCCCA AGGGAGCGGT TGTGGGACGC GGCAAGAAGA GGAAACAGAT CAAGGACCCC 
AATGCTCCAA AACGTTCATT GTCTGCGTTC TTCTGGTTCT GCAATGACGA GAGAAATAAG 
GTGAAGGCAC TTAATCCCGA GTTCGGAGTG GGCGACATCG CCAAAGAGCT GGGACGAAAG 
TGGTCCGATG TGGATCCCGA GGTCAAGCAG AAGTACGAGT CGATGGCCGA GCGGGACAAG 
GCTCGATATG AACGCGAGAT GACCGAGTAC AAGACGAGCG GGAAAATAGC CATGTCCGCA 
CCGTCGATGC AGGCGTCGAT GCAGGCGCAG GCACAGAAAG CCGCGTTACT TGCCGCCGCT 
GCCCAGCAGC AGCACCAGCA GCTGGAAGAG CAGCACGACG ACGACGACGG CGATGGAGAT 
GATGACGAGA ACCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542446.2
CDS	718..1875
Translation	MEHFHQIQQLAAQQQQQVQQQQLQQHQVVVQQNQQQAHQNSSNTTAGVGTQQLFTYKMASSFPNPATTMAQVVATSNAAGTTGYDYRLNMAQAAAAAAVPGSQWWYSAANQGQVDANTAAQLQHQQQQQQQQQQQQQQQHQQQQQMQQQQQQQNVINSASPMSRVKADAKPRGRMTAYAYFVQTCREEHKKKHPDETVIFAEFSRKCAERWKTMVDKEKKRFHEMAEKDKQRYEAEMQNYVPPKGAVVGRGKKRKQIKDPNAPKRSLSAFFWFCNDERNKVKALNPEFGVGDIAKELGRKWSDVDPEVKQKYESMAERDKARYEREMTEYKTSGKIAMSAPSMQASMQAQAQKAALLAAAAQQQHQQLEEQHDDDDGDGDDDENQ

Target ORF information:

RefSeq Version	NM_080715.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dorsal switch protein 1, transcript variant C (Dsp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080715.4

ORF Insert Sequence:

ATGGAACACT TTCATCAAAT ACAGCAATTG GCTGCTCAGC AACAACAACA AGTACAACAG 
CAACAGTTGC AACAACACCA GGTGGTCGTC CAGCAGAATC AGCAGCAGGC GCACCAGAAC 
AGCTCCAATA CCACAGCCGG CGTGGGCACC CAACAGCTGT TCACATACAA AATGGCCAGC 
AGTTTTCCGA ATCCGGCCAC GACGATGGCC CAAGTGGTGG CCACATCGAA CGCAGCCGGC 
ACCACGGGCT ATGATTATCG TCTAAATATG GCACAGGCAG CCGCAGCAGC AGCCGTACCC 
GGCTCCCAGT GGTGGTATTC GGCCGCCAAT CAGGGACAGG TCGATGCCAA TACAGCGGCG 
CAACTGCAGC ACCAGCAGCA GCAACAGCAA CAGCAACAAC AGCAGCAGCA GCAACAGCAC 
CAGCAGCAAC AACAGATGCA ACAGCAGCAG CAGCAACAGA ATGTCATCAA TTCGGCGAGC 
CCCATGAGCA GAGTCAAGGC CGATGCCAAG CCCAGAGGCC GAATGACCGC CTACGCATAC 
TTTGTACAAA CTTGCAGAGA GGAGCACAAA AAGAAACATC CCGATGAGAC GGTGATATTC 
GCCGAATTCT CGCGGAAGTG CGCCGAACGG TGGAAGACAA TGGTGGACAA GGAGAAGAAG 
CGCTTCCACG AAATGGCCGA GAAGGACAAG CAGCGATATG AGGCGGAAAT GCAGAACTAT 
GTGCCGCCCA AGGGAGCGGT TGTGGGACGC GGCAAGAAGA GGAAACAGAT CAAGGACCCC 
AATGCTCCAA AACGTTCATT GTCTGCGTTC TTCTGGTTCT GCAATGACGA GAGAAATAAG 
GTGAAGGCAC TTAATCCCGA GTTCGGAGTG GGCGACATCG CCAAAGAGCT GGGACGAAAG 
TGGTCCGATG TGGATCCCGA GGTCAAGCAG AAGTACGAGT CGATGGCCGA GCGGGACAAG 
GCTCGATATG AACGCGAGAT GACCGAGTAC AAGACGAGCG GGAAAATAGC CATGTCCGCA 
CCGTCGATGC AGGCGTCGAT GCAGGCGCAG GCACAGAAAG CCGCGTTACT TGCCGCCGCT 
GCCCAGCAGC AGCACCAGCA GCTGGAAGAG CAGCACGACG ACGACGACGG CGATGGAGAT 
GATGACGAGA ACCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsp1 ( NM_167502.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727959.2 Drosophila melanogaster dorsal switch protein 1, transcript variant D (Dsp1), mRNA.

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CloneID	OFa02476
Clone ID Related Accession (Same CDS sequence)	NM_167502.3 , NM_001298393.1
Accession Version	NM_167502.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dorsal switch protein 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_167502.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACACT TTCATCAAAT ACAGCAGCAA TTGGCTGCTC AGCAACAACA ACAAGTACAA 
CAGCAACAGT TGCAACAACA CCAGGTGGTC GTCCAGCAGA ATCAGCAGCA GGCGCACCAG 
AACAGCTCCA ATACCACAGC CGGCGTGGGC ACCCAACAGC TGTTCACATA CAAAATGGCC 
AGCAGTTTTC CGAATCCGGC CACGACGATG GCCCAAGTGG TGGCCACATC GAACGCAGCC 
GGCACCACGG GCTATGATTA TCGTCTAAAT ATGGCACAGG CAGCCGCAGC AGCAGCCGTA 
CCCGGCTCCC AGTGGTGGTA TTCGGCCGCC AATCAGGGAC AGGTCGATGC CAATACAGCG 
GCGCAACTGC AGCACCAGCA GCAGCAACAG CAACAGCAAC AACAGCAGCA GCAGCAACAG 
CACCAGCAGC AACAACAGAT GCAACAGCAG CAGCAGCAAC AGAATGTCAT CAATTCGGCG 
AGCCCCATGA GCAGAGTCAA GGCCGATGCC AAGCCCAGAG GCCGAATGAC CGCCTACGCA 
TACTTTGTAC AAACTTGCAG AGAGGAGCAC AAAAAGAAAC ATCCCGATGA GACGGTGATA 
TTCGCCGAAT TCTCGCGGAA GTGCGCCGAA CGGTGGAAGA CAATGGTGGA CAAGGAGAAG 
AAGCGCTTCC ACGAAATGGC CGAGAAGGAC AAGCAGCGAT ATGAGGCGGA AATGCAGAAC 
TATGTGCCGC CCAAGGGAGC GGTTGTGGGA CGCGGCAAGA AGAGGAAACA GATCAAGGAC 
CCCAATGCTC CAAAACGTTC ATTGTCTGCG TTCTTCTGGT TCTGCAATGA CGAGAGAAAT 
AAGGTGAAGG CACTTAATCC CGAGTTCGGA GTGGGCGACA TCGCCAAAGA GCTGGGACGA 
AAGTGGTCCG ATGTGGATCC CGAGGTCAAG CAGAAGTACG AGTCGATGGC CGAGCGGGAC 
AAGGCTCGAT ATGAACGCGA GATGACCGAG TACAAGACGA GCGGGAAAAT AGCCATGTCC 
GCACCGTCGA TGCAGGCGTC GATGCAGGCG CAGGCACAGA AAGCCGCGTT ACTTGCCGCC 
GCTGCCCAGC AGCAGCACCA GCAGCTGGAA GAGCAGCACG ACGACGACGA CGGCGATGGA 
GATGATGACG AGAACCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727959.2
CDS	779..1939
Translation	MEHFHQIQQQLAAQQQQQVQQQQLQQHQVVVQQNQQQAHQNSSNTTAGVGTQQLFTYKMASSFPNPATTMAQVVATSNAAGTTGYDYRLNMAQAAAAAAVPGSQWWYSAANQGQVDANTAAQLQHQQQQQQQQQQQQQQQHQQQQQMQQQQQQQNVINSASPMSRVKADAKPRGRMTAYAYFVQTCREEHKKKHPDETVIFAEFSRKCAERWKTMVDKEKKRFHEMAEKDKQRYEAEMQNYVPPKGAVVGRGKKRKQIKDPNAPKRSLSAFFWFCNDERNKVKALNPEFGVGDIAKELGRKWSDVDPEVKQKYESMAERDKARYEREMTEYKTSGKIAMSAPSMQASMQAQAQKAALLAAAAQQQHQQLEEQHDDDDGDGDDDENQ

Target ORF information:

RefSeq Version	NM_167502.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dorsal switch protein 1, transcript variant D (Dsp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167502.3

ORF Insert Sequence:

ATGGAACACT TTCATCAAAT ACAGCAGCAA TTGGCTGCTC AGCAACAACA ACAAGTACAA 
CAGCAACAGT TGCAACAACA CCAGGTGGTC GTCCAGCAGA ATCAGCAGCA GGCGCACCAG 
AACAGCTCCA ATACCACAGC CGGCGTGGGC ACCCAACAGC TGTTCACATA CAAAATGGCC 
AGCAGTTTTC CGAATCCGGC CACGACGATG GCCCAAGTGG TGGCCACATC GAACGCAGCC 
GGCACCACGG GCTATGATTA TCGTCTAAAT ATGGCACAGG CAGCCGCAGC AGCAGCCGTA 
CCCGGCTCCC AGTGGTGGTA TTCGGCCGCC AATCAGGGAC AGGTCGATGC CAATACAGCG 
GCGCAACTGC AGCACCAGCA GCAGCAACAG CAACAGCAAC AACAGCAGCA GCAGCAACAG 
CACCAGCAGC AACAACAGAT GCAACAGCAG CAGCAGCAAC AGAATGTCAT CAATTCGGCG 
AGCCCCATGA GCAGAGTCAA GGCCGATGCC AAGCCCAGAG GCCGAATGAC CGCCTACGCA 
TACTTTGTAC AAACTTGCAG AGAGGAGCAC AAAAAGAAAC ATCCCGATGA GACGGTGATA 
TTCGCCGAAT TCTCGCGGAA GTGCGCCGAA CGGTGGAAGA CAATGGTGGA CAAGGAGAAG 
AAGCGCTTCC ACGAAATGGC CGAGAAGGAC AAGCAGCGAT ATGAGGCGGA AATGCAGAAC 
TATGTGCCGC CCAAGGGAGC GGTTGTGGGA CGCGGCAAGA AGAGGAAACA GATCAAGGAC 
CCCAATGCTC CAAAACGTTC ATTGTCTGCG TTCTTCTGGT TCTGCAATGA CGAGAGAAAT 
AAGGTGAAGG CACTTAATCC CGAGTTCGGA GTGGGCGACA TCGCCAAAGA GCTGGGACGA 
AAGTGGTCCG ATGTGGATCC CGAGGTCAAG CAGAAGTACG AGTCGATGGC CGAGCGGGAC 
AAGGCTCGAT ATGAACGCGA GATGACCGAG TACAAGACGA GCGGGAAAAT AGCCATGTCC 
GCACCGTCGA TGCAGGCGTC GATGCAGGCG CAGGCACAGA AAGCCGCGTT ACTTGCCGCC 
GCTGCCCAGC AGCAGCACCA GCAGCTGGAA GAGCAGCACG ACGACGACGA CGGCGATGGA 
GATGATGACG AGAACCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsp1 ( NM_001298393.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285322.1 Drosophila melanogaster dorsal switch protein 1, transcript variant G (Dsp1), mRNA.

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CloneID	OFa02476
Clone ID Related Accession (Same CDS sequence)	NM_167502.3 , NM_001298393.1
Accession Version	NM_001298393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dorsal switch protein 1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACACT TTCATCAAAT ACAGCAGCAA TTGGCTGCTC AGCAACAACA ACAAGTACAA 
CAGCAACAGT TGCAACAACA CCAGGTGGTC GTCCAGCAGA ATCAGCAGCA GGCGCACCAG 
AACAGCTCCA ATACCACAGC CGGCGTGGGC ACCCAACAGC TGTTCACATA CAAAATGGCC 
AGCAGTTTTC CGAATCCGGC CACGACGATG GCCCAAGTGG TGGCCACATC GAACGCAGCC 
GGCACCACGG GCTATGATTA TCGTCTAAAT ATGGCACAGG CAGCCGCAGC AGCAGCCGTA 
CCCGGCTCCC AGTGGTGGTA TTCGGCCGCC AATCAGGGAC AGGTCGATGC CAATACAGCG 
GCGCAACTGC AGCACCAGCA GCAGCAACAG CAACAGCAAC AACAGCAGCA GCAGCAACAG 
CACCAGCAGC AACAACAGAT GCAACAGCAG CAGCAGCAAC AGAATGTCAT CAATTCGGCG 
AGCCCCATGA GCAGAGTCAA GGCCGATGCC AAGCCCAGAG GCCGAATGAC CGCCTACGCA 
TACTTTGTAC AAACTTGCAG AGAGGAGCAC AAAAAGAAAC ATCCCGATGA GACGGTGATA 
TTCGCCGAAT TCTCGCGGAA GTGCGCCGAA CGGTGGAAGA CAATGGTGGA CAAGGAGAAG 
AAGCGCTTCC ACGAAATGGC CGAGAAGGAC AAGCAGCGAT ATGAGGCGGA AATGCAGAAC 
TATGTGCCGC CCAAGGGAGC GGTTGTGGGA CGCGGCAAGA AGAGGAAACA GATCAAGGAC 
CCCAATGCTC CAAAACGTTC ATTGTCTGCG TTCTTCTGGT TCTGCAATGA CGAGAGAAAT 
AAGGTGAAGG CACTTAATCC CGAGTTCGGA GTGGGCGACA TCGCCAAAGA GCTGGGACGA 
AAGTGGTCCG ATGTGGATCC CGAGGTCAAG CAGAAGTACG AGTCGATGGC CGAGCGGGAC 
AAGGCTCGAT ATGAACGCGA GATGACCGAG TACAAGACGA GCGGGAAAAT AGCCATGTCC 
GCACCGTCGA TGCAGGCGTC GATGCAGGCG CAGGCACAGA AAGCCGCGTT ACTTGCCGCC 
GCTGCCCAGC AGCAGCACCA GCAGCTGGAA GAGCAGCACG ACGACGACGA CGGCGATGGA 
GATGATGACG AGAACCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285322.1
CDS	122..1282
Translation	MEHFHQIQQQLAAQQQQQVQQQQLQQHQVVVQQNQQQAHQNSSNTTAGVGTQQLFTYKMASSFPNPATTMAQVVATSNAAGTTGYDYRLNMAQAAAAAAVPGSQWWYSAANQGQVDANTAAQLQHQQQQQQQQQQQQQQQHQQQQQMQQQQQQQNVINSASPMSRVKADAKPRGRMTAYAYFVQTCREEHKKKHPDETVIFAEFSRKCAERWKTMVDKEKKRFHEMAEKDKQRYEAEMQNYVPPKGAVVGRGKKRKQIKDPNAPKRSLSAFFWFCNDERNKVKALNPEFGVGDIAKELGRKWSDVDPEVKQKYESMAERDKARYEREMTEYKTSGKIAMSAPSMQASMQAQAQKAALLAAAAQQQHQQLEEQHDDDDGDGDDDENQ

Target ORF information:

RefSeq Version	NM_001298393.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dorsal switch protein 1, transcript variant G (Dsp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298393.1

ORF Insert Sequence:

ATGGAACACT TTCATCAAAT ACAGCAGCAA TTGGCTGCTC AGCAACAACA ACAAGTACAA 
CAGCAACAGT TGCAACAACA CCAGGTGGTC GTCCAGCAGA ATCAGCAGCA GGCGCACCAG 
AACAGCTCCA ATACCACAGC CGGCGTGGGC ACCCAACAGC TGTTCACATA CAAAATGGCC 
AGCAGTTTTC CGAATCCGGC CACGACGATG GCCCAAGTGG TGGCCACATC GAACGCAGCC 
GGCACCACGG GCTATGATTA TCGTCTAAAT ATGGCACAGG CAGCCGCAGC AGCAGCCGTA 
CCCGGCTCCC AGTGGTGGTA TTCGGCCGCC AATCAGGGAC AGGTCGATGC CAATACAGCG 
GCGCAACTGC AGCACCAGCA GCAGCAACAG CAACAGCAAC AACAGCAGCA GCAGCAACAG 
CACCAGCAGC AACAACAGAT GCAACAGCAG CAGCAGCAAC AGAATGTCAT CAATTCGGCG 
AGCCCCATGA GCAGAGTCAA GGCCGATGCC AAGCCCAGAG GCCGAATGAC CGCCTACGCA 
TACTTTGTAC AAACTTGCAG AGAGGAGCAC AAAAAGAAAC ATCCCGATGA GACGGTGATA 
TTCGCCGAAT TCTCGCGGAA GTGCGCCGAA CGGTGGAAGA CAATGGTGGA CAAGGAGAAG 
AAGCGCTTCC ACGAAATGGC CGAGAAGGAC AAGCAGCGAT ATGAGGCGGA AATGCAGAAC 
TATGTGCCGC CCAAGGGAGC GGTTGTGGGA CGCGGCAAGA AGAGGAAACA GATCAAGGAC 
CCCAATGCTC CAAAACGTTC ATTGTCTGCG TTCTTCTGGT TCTGCAATGA CGAGAGAAAT 
AAGGTGAAGG CACTTAATCC CGAGTTCGGA GTGGGCGACA TCGCCAAAGA GCTGGGACGA 
AAGTGGTCCG ATGTGGATCC CGAGGTCAAG CAGAAGTACG AGTCGATGGC CGAGCGGGAC 
AAGGCTCGAT ATGAACGCGA GATGACCGAG TACAAGACGA GCGGGAAAAT AGCCATGTCC 
GCACCGTCGA TGCAGGCGTC GATGCAGGCG CAGGCACAGA AAGCCGCGTT ACTTGCCGCC 
GCTGCCCAGC AGCAGCACCA GCAGCTGGAA GAGCAGCACG ACGACGACGA CGGCGATGGA 
GATGATGACG AGAACCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsp1 ( NM_001144731.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138203.1 Drosophila melanogaster dorsal switch protein 1, transcript variant F (Dsp1), mRNA.

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CloneID	OFa02477
Clone ID Related Accession (Same CDS sequence)	NM_001144731.3
Accession Version	NM_001144731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dorsal switch protein 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001144731.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTAATGA ACATCCATCA ACCAACTATT GATCAGCAAA CAATTCAACA CTATCAGCAG 
CAATTGGCTG CTCAGCAACA ACAACAAGTA CAACAGCAAC AGTTGCAACA ACACCAGGTG 
GTCGTCCAGC AGAATCAGCA GCAGGCGCAC CAGAACAGCT CCAATACCAC AGCCGGCGTG 
GGCACCCAAC AGCTGTTCAC ATACAAAATG GCCAGCAGTT TTCCGAATCC GGCCACGACG 
ATGGCCCAAG TGGTGGCCAC ATCGAACGCA GCCGGCACCA CGGGCTATGA TTATCGTCTA 
AATATGGCAC AGGCAGCCGC AGCAGCAGCC GTACCCGGCT CCCAGTGGTG GTATTCGGCC 
GCCAATCAGG GACAGGTCGA TGCCAATACA GCGGCGCAAC TGCAGCACCA GCAGCAGCAA 
CAGCAACAGC AACAACAGCA GCAGCAGCAA CAGCACCAGC AGCAACAACA GATGCAACAG 
CAGCAGCAGC AACAGAATGT CATCAATTCG GCGAGCCCCA TGAGCAGAGT CAAGGCCGAT 
GCCAAGCCCA GAGGCCGAAT GACCGCCTAC GCATACTTTG TACAAACTTG CAGAGAGGAG 
CACAAAAAGA AACATCCCGA TGAGACGGTG ATATTCGCCG AATTCTCGCG GAAGTGCGCC 
GAACGGTGGA AGACAATGGT GGACAAGGAG AAGAAGCGCT TCCACGAAAT GGCCGAGAAG 
GACAAGCAGC GATATGAGGC GGAAATGCAG AACTATGTGC CGCCCAAGGG AGCGGTTGTG 
GGACGCGGCA AGAAGAGGAA ACAGATCAAG GACCCCAATG CTCCAAAACG TTCATTGTCT 
GCGTTCTTCT GGTTCTGCAA TGACGAGAGA AATAAGGTGA AGGCACTTAA TCCCGAGTTC 
GGAGTGGGCG ACATCGCCAA AGAGCTGGGA CGAAAGTGGT CCGATGTGGA TCCCGAGGTC 
AAGCAGAAGT ACGAGTCGAT GGCCGAGCGG GACAAGGCTC GATATGAACG CGAGATGACC 
GAGTACAAGA CGAGCGGGAA AATAGCCATG TCCGCACCGT CGATGCAGGC GTCGATGCAG 
GCGCAGGCAC AGAAAGCCGC GTTACTTGCC GCCGCTGCCC AGCAGCAGCA CCAGCAGCTG 
GAAGAGCAGC ACGACGACGA CGACGGCGAT GGAGATGATG ACGAGAACCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138203.1
CDS	48..1241
Translation	MLMNIHQPTIDQQTIQHYQQQLAAQQQQQVQQQQLQQHQVVVQQNQQQAHQNSSNTTAGVGTQQLFTYKMASSFPNPATTMAQVVATSNAAGTTGYDYRLNMAQAAAAAAVPGSQWWYSAANQGQVDANTAAQLQHQQQQQQQQQQQQQQQHQQQQQMQQQQQQQNVINSASPMSRVKADAKPRGRMTAYAYFVQTCREEHKKKHPDETVIFAEFSRKCAERWKTMVDKEKKRFHEMAEKDKQRYEAEMQNYVPPKGAVVGRGKKRKQIKDPNAPKRSLSAFFWFCNDERNKVKALNPEFGVGDIAKELGRKWSDVDPEVKQKYESMAERDKARYEREMTEYKTSGKIAMSAPSMQASMQAQAQKAALLAAAAQQQHQQLEEQHDDDDGDGDDDENQ

Target ORF information:

RefSeq Version	NM_001144731.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dorsal switch protein 1, transcript variant F (Dsp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144731.3

ORF Insert Sequence:

ATGCTAATGA ACATCCATCA ACCAACTATT GATCAGCAAA CAATTCAACA CTATCAGCAG 
CAATTGGCTG CTCAGCAACA ACAACAAGTA CAACAGCAAC AGTTGCAACA ACACCAGGTG 
GTCGTCCAGC AGAATCAGCA GCAGGCGCAC CAGAACAGCT CCAATACCAC AGCCGGCGTG 
GGCACCCAAC AGCTGTTCAC ATACAAAATG GCCAGCAGTT TTCCGAATCC GGCCACGACG 
ATGGCCCAAG TGGTGGCCAC ATCGAACGCA GCCGGCACCA CGGGCTATGA TTATCGTCTA 
AATATGGCAC AGGCAGCCGC AGCAGCAGCC GTACCCGGCT CCCAGTGGTG GTATTCGGCC 
GCCAATCAGG GACAGGTCGA TGCCAATACA GCGGCGCAAC TGCAGCACCA GCAGCAGCAA 
CAGCAACAGC AACAACAGCA GCAGCAGCAA CAGCACCAGC AGCAACAACA GATGCAACAG 
CAGCAGCAGC AACAGAATGT CATCAATTCG GCGAGCCCCA TGAGCAGAGT CAAGGCCGAT 
GCCAAGCCCA GAGGCCGAAT GACCGCCTAC GCATACTTTG TACAAACTTG CAGAGAGGAG 
CACAAAAAGA AACATCCCGA TGAGACGGTG ATATTCGCCG AATTCTCGCG GAAGTGCGCC 
GAACGGTGGA AGACAATGGT GGACAAGGAG AAGAAGCGCT TCCACGAAAT GGCCGAGAAG 
GACAAGCAGC GATATGAGGC GGAAATGCAG AACTATGTGC CGCCCAAGGG AGCGGTTGTG 
GGACGCGGCA AGAAGAGGAA ACAGATCAAG GACCCCAATG CTCCAAAACG TTCATTGTCT 
GCGTTCTTCT GGTTCTGCAA TGACGAGAGA AATAAGGTGA AGGCACTTAA TCCCGAGTTC 
GGAGTGGGCG ACATCGCCAA AGAGCTGGGA CGAAAGTGGT CCGATGTGGA TCCCGAGGTC 
AAGCAGAAGT ACGAGTCGAT GGCCGAGCGG GACAAGGCTC GATATGAACG CGAGATGACC 
GAGTACAAGA CGAGCGGGAA AATAGCCATG TCCGCACCGT CGATGCAGGC GTCGATGCAG 
GCGCAGGCAC AGAAAGCCGC GTTACTTGCC GCCGCTGCCC AGCAGCAGCA CCAGCAGCTG 
GAAGAGCAGC ACGACGACGA CGACGGCGAT GGAGATGATG ACGAGAACCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dsp1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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