LOC109546131 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109546131
Entrez Gene ID	109546131
Full Name	N6-adenosine-methyltransferase subunit METTL3
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019916961.1	XP_019772520.1	uncharacterized protein LOC109546131 isoform X2
XM_019916960.1	XP_019772519.1	N6-adenosine-methyltransferase subunit METTL3 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC109546131 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109546131 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe35083	XM_019916960.1 Latest version!	Dendroctonus ponderosae N6-adenosine-methyltransferase subunit METTL3 (LOC109546131), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODe35084	XM_019916961.1 Latest version!	Dendroctonus ponderosae N6-adenosine-methyltransferase subunit METTL3 (LOC109546131), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109546131 ( XM_019916960.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019772519.1 Dendroctonus ponderosae N6-adenosine-methyltransferase subunit METTL3 (LOC109546131), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODe35083
Clone ID Related Accession (Same CDS sequence)	XM_019916960.1
Accession Version	XM_019916960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	N6-adenosine-methyltransferase subunit METTL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017851077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACG CATGGGAGGA AATTCAGGCG GTGCAACGGA AGCGCAACAG CCTCAGGGAG 
CGACTGGAAA AGCGCAAAAA GGAGCGCCGA GACATTCTGG GGTCCAGCTT GCATAGCTGC 
AACAGCTCAG ACTTGGCGAG TTCTACCTCT GCACCCGGGC TTTCAAAGGA GGAGGTGAAG 
CCAAAAAACG AGGACGGCTT TGAGGCCCAA TTGCTGAAAG TCGACCCCGA GCTGGAAAAA 
GAGCTGCTCA GGCACCTGGC GGATGTTACA CTACAACTAC CGATGTGTTC GTTCGATTTA 
GTCGCCACCC TCAAGACCAG CGCCTCACAT AAACAAGTCA GCAATTTGCT GCAGAAATTC 
GCCACTCAGA AGCTGATTCT AGTGAAAGAC ACCGTTAGAG ATGGAGTGAA CACGCTCGAT 
GTAGCCTCAG TTGAAGTTTC AAAGGTCAAT GCGATGATTG CCGAGCTATT TGATGAACAA 
CAGCCCAACG TGGGGGGAAC AGCCAAGCGG AAAAGGGAGG AAGCCGAAGC TGTGGAGAGT 
CTGGAGGAGG AGAGACGCAA AAAAGAGAAG AAGGAACCAA AGACTGACAT TCTGTCGCTG 
CTGTCGATGC CCTCCACTAA GGAGAAGGAA ACCAAGAAGA TCGGCGAGGA GATTTTGGTG 
CTGCTTAGTA AACCCACTGC CAAAGAACGA TCGCTGACCG AAAGATTCAA GTCGCAAGGT 
GGTACACAGC TTATGGAGTT CTGTCCACAT GGAACCAGGT TGGATTGTGA AAAAAATTCA 
ATCACCGATT GCAAGAAGCT ACACTTCAAG AAAATAATCC AAAAACACAC GGATGAGTCG 
TTGGGGGATT GTTCTTTTCT CAATACCTGC TTCCATATGG ACACGTGTAA ATACGTGCAT 
TACGAAGTGG ATAGGGCAGC CCAAATCCAT GGCAATCAGT TGGTCATTCC CAACCTACCA 
GCAGTTGGAC GAGTGGAAAG CACCACACTG TATCCACCGC AATGGGTGCA ATGCGACCTG 
CGGTACCTGG ATATGACCGT GTTAGGAAAA TTTGCAGTGA TAATGGCTGA TCCGCCTTGG 
GACATCCACA TGGAACTCCC ATATGGAACA ATGTCCGACG ATGAAATGAG ACAACTGGGT 
ATACCGCAAC TGCAAGACGA AGGACTGATA TTCTTGTGGG TTACTGGACG AGCTATGGAA 
CTGGGCAGGG AGTGCCTCAA GTTGTGGGGC TACGAACGAG TGGATGAAAT CATCTGGGTC 
AAGACCAATC AGTTGCAAAG AATCATCAGA ACTGGTCGTA CTGGACATTG GTTGAACCAT 
GGCAAAGAGC ATTGCCTAGT GGGAATGAAA GGTAACCCGA AGGACCTGAA TCGAGGCCTC 
GACTCGGACG TAATCGTGGC AGAAGTGCGT GCAACCAGTC ACAAGCCGGA TGAGATCTAC 
GGGATGATCG AAAGATTATC GCCCGGTACC AGGAAGATTG AGCTGTTTGG CCGTCCGCAC 
AACATCCAAC CAAACTGGAT CACCTTAGGC AATCAAGTGG ATGGAATCCG ACTCTTAGAC 
CAGGATTTGA TTGAAAGTTT CAAGAAGCGG TATCCGACGG GCAACTGTAT GGCCCCGCCG 
CCTGAAAGTG TTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019772519.1
CDS	922..2559
Translation	MSDAWEEIQAVQRKRNSLRERLEKRKKERRDILGSSLHSCNSSDLASSTSAPGLSKEEVKPKNEDGFEAQLLKVDPELEKELLRHLADVTLQLPMCSFDLVATLKTSASHKQVSNLLQKFATQKLILVKDTVRDGVNTLDVASVEVSKVNAMIAELFDEQQPNVGGTAKRKREEAEAVESLEEERRKKEKKEPKTDILSLLSMPSTKEKETKKIGEEILVLLSKPTAKERSLTERFKSQGGTQLMEFCPHGTRLDCEKNSITDCKKLHFKKIIQKHTDESLGDCSFLNTCFHMDTCKYVHYEVDRAAQIHGNQLVIPNLPAVGRVESTTLYPPQWVQCDLRYLDMTVLGKFAVIMADPPWDIHMELPYGTMSDDEMRQLGIPQLQDEGLIFLWVTGRAMELGRECLKLWGYERVDEIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGMKGNPKDLNRGLDSDVIVAEVRATSHKPDEIYGMIERLSPGTRKIELFGRPHNIQPNWITLGNQVDGIRLLDQDLIESFKKRYPTGNCMAPPPESVH

Target ORF information:

RefSeq Version	XM_019916960.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae N6-adenosine-methyltransferase subunit METTL3 (LOC109546131), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019916960.1

ORF Insert Sequence:

ATGTCGGACG CATGGGAGGA AATTCAGGCG GTGCAACGGA AGCGCAACAG CCTCAGGGAG 
CGACTGGAAA AGCGCAAAAA GGAGCGCCGA GACATTCTGG GGTCCAGCTT GCATAGCTGC 
AACAGCTCAG ACTTGGCGAG TTCTACCTCT GCACCCGGGC TTTCAAAGGA GGAGGTGAAG 
CCAAAAAACG AGGACGGCTT TGAGGCCCAA TTGCTGAAAG TCGACCCCGA GCTGGAAAAA 
GAGCTGCTCA GGCACCTGGC GGATGTTACA CTACAACTAC CGATGTGTTC GTTCGATTTA 
GTCGCCACCC TCAAGACCAG CGCCTCACAT AAACAAGTCA GCAATTTGCT GCAGAAATTC 
GCCACTCAGA AGCTGATTCT AGTGAAAGAC ACCGTTAGAG ATGGAGTGAA CACGCTCGAT 
GTAGCCTCAG TTGAAGTTTC AAAGGTCAAT GCGATGATTG CCGAGCTATT TGATGAACAA 
CAGCCCAACG TGGGGGGAAC AGCCAAGCGG AAAAGGGAGG AAGCCGAAGC TGTGGAGAGT 
CTGGAGGAGG AGAGACGCAA AAAAGAGAAG AAGGAACCAA AGACTGACAT TCTGTCGCTG 
CTGTCGATGC CCTCCACTAA GGAGAAGGAA ACCAAGAAGA TCGGCGAGGA GATTTTGGTG 
CTGCTTAGTA AACCCACTGC CAAAGAACGA TCGCTGACCG AAAGATTCAA GTCGCAAGGT 
GGTACACAGC TTATGGAGTT CTGTCCACAT GGAACCAGGT TGGATTGTGA AAAAAATTCA 
ATCACCGATT GCAAGAAGCT ACACTTCAAG AAAATAATCC AAAAACACAC GGATGAGTCG 
TTGGGGGATT GTTCTTTTCT CAATACCTGC TTCCATATGG ACACGTGTAA ATACGTGCAT 
TACGAAGTGG ATAGGGCAGC CCAAATCCAT GGCAATCAGT TGGTCATTCC CAACCTACCA 
GCAGTTGGAC GAGTGGAAAG CACCACACTG TATCCACCGC AATGGGTGCA ATGCGACCTG 
CGGTACCTGG ATATGACCGT GTTAGGAAAA TTTGCAGTGA TAATGGCTGA TCCGCCTTGG 
GACATCCACA TGGAACTCCC ATATGGAACA ATGTCCGACG ATGAAATGAG ACAACTGGGT 
ATACCGCAAC TGCAAGACGA AGGACTGATA TTCTTGTGGG TTACTGGACG AGCTATGGAA 
CTGGGCAGGG AGTGCCTCAA GTTGTGGGGC TACGAACGAG TGGATGAAAT CATCTGGGTC 
AAGACCAATC AGTTGCAAAG AATCATCAGA ACTGGTCGTA CTGGACATTG GTTGAACCAT 
GGCAAAGAGC ATTGCCTAGT GGGAATGAAA GGTAACCCGA AGGACCTGAA TCGAGGCCTC 
GACTCGGACG TAATCGTGGC AGAAGTGCGT GCAACCAGTC ACAAGCCGGA TGAGATCTAC 
GGGATGATCG AAAGATTATC GCCCGGTACC AGGAAGATTG AGCTGTTTGG CCGTCCGCAC 
AACATCCAAC CAAACTGGAT CACCTTAGGC AATCAAGTGG ATGGAATCCG ACTCTTAGAC 
CAGGATTTGA TTGAAAGTTT CAAGAAGCGG TATCCGACGG GCAACTGTAT GGCCCCGCCG 
CCTGAAAGTG TTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109546131 ( XM_019916961.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019772520.1 Dendroctonus ponderosae N6-adenosine-methyltransferase subunit METTL3 (LOC109546131), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODe35084
Clone ID Related Accession (Same CDS sequence)	XM_019916961.1
Accession Version	XM_019916961.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	uncharacterized protein LOC109546131 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017851077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACG CATGGGAGGA AATTCAGGCG GTGCAACGGA AGCGCAACAG CCTCAGGGAG 
CGACTGGAAA AGCGCAAAAA GGAGCGCCGA GACATTCTGG GGTCCAGCTT GCATAGCTGC 
AACAGCTCAG ACTTGGCGAG TTCTACCTCT GCACCCGGGC TTTCAAAGGA GGAGGTGAAG 
CCAAAAAACG AGGACGGCTT TGAGGCCCAA TTGCTGAAAG TCGACCCCGA GCTGGAAAAA 
GAGCTGCTCA GGCACCTGGC GGATGTTACA CTACAACTAC CGATGTGTTC GTTCGATTTA 
GTCGCCACCC TCAAGACCAG CGCCTCACAT AAACAAGTCA GCAATTTGCT GCAGAAATTC 
GCCACTCAGA AGCTGATTCT AGTGAAAGAC ACCGTTAGAG ATGGAGTGAA CACGCTCGAT 
GTAGCCTCAG TTGAAGTTTC AAAGGTCAAT GCGATGATTG CCGAGCTATT TGATGAACAA 
CAGCCCAACG TGGGGGGAAC AGCCAAGCGG AAAAGGGAGG AAGCCGAAGC TGTGGAGAGT 
CTGGAGGAGG AGAGACGCAA AAAAGAGAAG AAGGAACCAA AGACTGACAT TCTGTCGCTG 
CTGTCGATGC CCTCCACTAA GGAGAAGGAA ACCAAGAAGA TCGGCGAGGA GATTTTGGTG 
CTGCTTAGTA AACCCACTGC CAAAGAACGA TCGCTGACCG AAAGATTCAA GTCGCAAGAG 
TACGAAGAAC CAATCGTAGG AACGGGAATA CCAGAAATCG AGACAGAAGG ACGTGCGTTG 
TGTTCCGTGA AGTTGGAAGA GAGGGCGATA AAGCTCTCAC AAACTCGCCC AAGTCAACAA 
CAAGATCAGG CGTTAGACTT CCTCTCAACA CTGATTCAGT CGGACGTGAC ACCGAATGTA 
GACGTTTTAA ATGAACTGGC ATTAGCATTG GGCGAACAAC CTTTACACAC ATTTCCTGTA 
AAACCCTACC TGGAAAAAGA ACTGTTCGAA ACTTCCTATG ATTTTATCTC GTATGCTTCG 
TTATTTCGAG TGAACAACGC GGACTTTTCA ATCGTCCAAA TGAAGTCGGT GCATAATATT 
TTTAATTCCC TACATTACCG ACTAGCAAAA CAGAAGTATG ATACAGTTTA CAAGGAAGGA 
TTTACCGAGA GTTTGTTGTT TCATGGAACA AGGCAAGATT TTGTGAATGG AATTTGCACT 
TACAACTTTG ACAGATCTAA GATAAAAACG CACAAGTTTG GTCGTGGAAT TTCATTCGCC 
ACACAGTCCT TCTACGCCAC GCACTACCAT CGTGACAATA AGAGGCGTCA AGACAAGGTA 
ATGGTAATAG CAAAGGTACT GGTGGGACGT AGATTCGAGA TAGGCAGCCC GACAATGAAC 
ATTCCGAAGA AAATGTGCTA CACTTCAAAA AACGGCGAGC AAACGGTCAT TGTCAAGTAT 
GACGATCACA CGTTTTTGCC AGTCGCTATT GTTCATTACC GAGGACTACA CCTGCCAAGC 
AGGTCTTACA GGTCTATATT TTCTTATTTT GCAAAGATGT ATCGCATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019772520.1
CDS	922..2472
Translation	MSDAWEEIQAVQRKRNSLRERLEKRKKERRDILGSSLHSCNSSDLASSTSAPGLSKEEVKPKNEDGFEAQLLKVDPELEKELLRHLADVTLQLPMCSFDLVATLKTSASHKQVSNLLQKFATQKLILVKDTVRDGVNTLDVASVEVSKVNAMIAELFDEQQPNVGGTAKRKREEAEAVESLEEERRKKEKKEPKTDILSLLSMPSTKEKETKKIGEEILVLLSKPTAKERSLTERFKSQEYEEPIVGTGIPEIETEGRALCSVKLEERAIKLSQTRPSQQQDQALDFLSTLIQSDVTPNVDVLNELALALGEQPLHTFPVKPYLEKELFETSYDFISYASLFRVNNADFSIVQMKSVHNIFNSLHYRLAKQKYDTVYKEGFTESLLFHGTRQDFVNGICTYNFDRSKIKTHKFGRGISFATQSFYATHYHRDNKRRQDKVMVIAKVLVGRRFEIGSPTMNIPKKMCYTSKNGEQTVIVKYDDHTFLPVAIVHYRGLHLPSRSYRSIFSYFAKMYRM

Target ORF information:

RefSeq Version	XM_019916961.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae N6-adenosine-methyltransferase subunit METTL3 (LOC109546131), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019916961.1

ORF Insert Sequence:

ATGTCGGACG CATGGGAGGA AATTCAGGCG GTGCAACGGA AGCGCAACAG CCTCAGGGAG 
CGACTGGAAA AGCGCAAAAA GGAGCGCCGA GACATTCTGG GGTCCAGCTT GCATAGCTGC 
AACAGCTCAG ACTTGGCGAG TTCTACCTCT GCACCCGGGC TTTCAAAGGA GGAGGTGAAG 
CCAAAAAACG AGGACGGCTT TGAGGCCCAA TTGCTGAAAG TCGACCCCGA GCTGGAAAAA 
GAGCTGCTCA GGCACCTGGC GGATGTTACA CTACAACTAC CGATGTGTTC GTTCGATTTA 
GTCGCCACCC TCAAGACCAG CGCCTCACAT AAACAAGTCA GCAATTTGCT GCAGAAATTC 
GCCACTCAGA AGCTGATTCT AGTGAAAGAC ACCGTTAGAG ATGGAGTGAA CACGCTCGAT 
GTAGCCTCAG TTGAAGTTTC AAAGGTCAAT GCGATGATTG CCGAGCTATT TGATGAACAA 
CAGCCCAACG TGGGGGGAAC AGCCAAGCGG AAAAGGGAGG AAGCCGAAGC TGTGGAGAGT 
CTGGAGGAGG AGAGACGCAA AAAAGAGAAG AAGGAACCAA AGACTGACAT TCTGTCGCTG 
CTGTCGATGC CCTCCACTAA GGAGAAGGAA ACCAAGAAGA TCGGCGAGGA GATTTTGGTG 
CTGCTTAGTA AACCCACTGC CAAAGAACGA TCGCTGACCG AAAGATTCAA GTCGCAAGAG 
TACGAAGAAC CAATCGTAGG AACGGGAATA CCAGAAATCG AGACAGAAGG ACGTGCGTTG 
TGTTCCGTGA AGTTGGAAGA GAGGGCGATA AAGCTCTCAC AAACTCGCCC AAGTCAACAA 
CAAGATCAGG CGTTAGACTT CCTCTCAACA CTGATTCAGT CGGACGTGAC ACCGAATGTA 
GACGTTTTAA ATGAACTGGC ATTAGCATTG GGCGAACAAC CTTTACACAC ATTTCCTGTA 
AAACCCTACC TGGAAAAAGA ACTGTTCGAA ACTTCCTATG ATTTTATCTC GTATGCTTCG 
TTATTTCGAG TGAACAACGC GGACTTTTCA ATCGTCCAAA TGAAGTCGGT GCATAATATT 
TTTAATTCCC TACATTACCG ACTAGCAAAA CAGAAGTATG ATACAGTTTA CAAGGAAGGA 
TTTACCGAGA GTTTGTTGTT TCATGGAACA AGGCAAGATT TTGTGAATGG AATTTGCACT 
TACAACTTTG ACAGATCTAA GATAAAAACG CACAAGTTTG GTCGTGGAAT TTCATTCGCC 
ACACAGTCCT TCTACGCCAC GCACTACCAT CGTGACAATA AGAGGCGTCA AGACAAGGTA 
ATGGTAATAG CAAAGGTACT GGTGGGACGT AGATTCGAGA TAGGCAGCCC GACAATGAAC 
ATTCCGAAGA AAATGTGCTA CACTTCAAAA AACGGCGAGC AAACGGTCAT TGTCAAGTAT 
GACGATCACA CGTTTTTGCC AGTCGCTATT GTTCATTACC GAGGACTACA CCTGCCAAGC 
AGGTCTTACA GGTCTATATT TTCTTATTTT GCAAAGATGT ATCGCATGTA A