LOC109534327 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109534327
Entrez Gene ID	109534327
Full Name	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019899957.1	XP_019755516.1	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho

Pathways from BioSystems
Gene Ontology

The following LOC109534327 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109534327 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe37770	XM_019899957.1 Latest version!	Dendroctonus ponderosae tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho (LOC109534327), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109534327 ( XM_019899957.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019755516.1 Dendroctonus ponderosae tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho (LOC109534327), mRNA.

Price & Availability↑

CloneID	ODe37770
Clone ID Related Accession (Same CDS sequence)	XM_019899957.1
Accession Version	XM_019899957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017851724.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTATAA TACAGGAAGA CCTCCAAACT GCTGATAGGT TTAAGCTGGT GACCTCCGCT 
TTGGATCGGC TGGTTGTTAT TGACGCTCAA GCCAATTCCC AATGCATTGA CCTGCCAAAG 
CTCCCGGCTG CAGAGCAGGA TGATAAAGTG GGTCAAATCA AGCCAACTGA AGCCGTAATC 
AACCGGATCA CCTGCATGGA GTCTTCCAGA GATCAGCAGT ACATAGCAGT GACCACTGAA 
AGCAAGCAGT TGGTGGTCTT TGGGGCCCAT TTCGGCGTGG TGAAGAACCT GATTTTGGCG 
CGAGCGCCTA GCAAGGTGTG CTTTACGCCA ACCAACGATG TATTAGTGGC CGATAGGACT 
GGGGATGTTT ACCTGTATAA AATAGCAGAC GAAAGTAATC AGCCGCAGTT GCTACTGGGA 
CATTTAAGTG TGATTCTGGA CATGTTGGTA ACAACCTGCG GCCAGTACGT GATAACTTGC 
GACCGAGACG AGAAGATCCG CGTGTCCCGT TATCCGAACT CGTATAACAT TGCCACTTTC 
TGCTTGGGGC ATACGGAGTT TGTTACTTCC CTGGCTTTGC TAACTAATCC CAACGTCCTC 
CTGTCGGCTT CGGGGGATGG GACTGTTCGG ATGTGGCACT ACTCGGAGGG CAAACAAATT 
AGCATAATAA ACACGGCTGG TTTGGTGGAT GGGCTCACTA TGGAAAAGTT CGGCAGCCAA 
ATGGATCTGG AAAAAGTGGA CGTAACTGCC CTCCCAGTAA CCAGCATGAA AGTGAGCGAA 
CAAAACGGAC TGGTAACTCT AGCTATTTCC TTATACAGTA TTCCCAAACT GTATCTATAC 
AGCATTAAGA CGCCCAGTTT AGAACATTCG CTTAAGCATA CTGTGGATTT GTATGCCAAC 
ATTGTCTCTT ATTGGCTAAC AAGTCGGCTC TATGTGCTCT CAGATAAGTT TGAGGTATAT 
CAAGTTGACG CTGATACTCT TAGGGCAGTG GACGCGAAAA CTACCCAAAA TAGACTAGTT 
GAGTATAAAG CGGCCTTTGT AAGTAACGAC TGCGACGTTA CAGCGTGGTA TAAAAGGAAG 
TTTGATAACG TTCAGGAATA CATGGAGCGA AAAAAGCTGA GGCTGGAGTC GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019755516.1
CDS	93..1229
Translation	MFIIQEDLQTADRFKLVTSALDRLVVIDAQANSQCIDLPKLPAAEQDDKVGQIKPTEAVINRITCMESSRDQQYIAVTTESKQLVVFGAHFGVVKNLILARAPSKVCFTPTNDVLVADRTGDVYLYKIADESNQPQLLLGHLSVILDMLVTTCGQYVITCDRDEKIRVSRYPNSYNIATFCLGHTEFVTSLALLTNPNVLLSASGDGTVRMWHYSEGKQISIINTAGLVDGLTMEKFGSQMDLEKVDVTALPVTSMKVSEQNGLVTLAISLYSIPKLYLYSIKTPSLEHSLKHTVDLYANIVSYWLTSRLYVLSDKFEVYQVDADTLRAVDAKTTQNRLVEYKAAFVSNDCDVTAWYKRKFDNVQEYMERKKLRLESK

Target ORF information:

RefSeq Version	XM_019899957.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho (LOC109534327), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019899957.1

ORF Insert Sequence:

ATGTTTATAA TACAGGAAGA CCTCCAAACT GCTGATAGGT TTAAGCTGGT GACCTCCGCT 
TTGGATCGGC TGGTTGTTAT TGACGCTCAA GCCAATTCCC AATGCATTGA CCTGCCAAAG 
CTCCCGGCTG CAGAGCAGGA TGATAAAGTG GGTCAAATCA AGCCAACTGA AGCCGTAATC 
AACCGGATCA CCTGCATGGA GTCTTCCAGA GATCAGCAGT ACATAGCAGT GACCACTGAA 
AGCAAGCAGT TGGTGGTCTT TGGGGCCCAT TTCGGCGTGG TGAAGAACCT GATTTTGGCG 
CGAGCGCCTA GCAAGGTGTG CTTTACGCCA ACCAACGATG TATTAGTGGC CGATAGGACT 
GGGGATGTTT ACCTGTATAA AATAGCAGAC GAAAGTAATC AGCCGCAGTT GCTACTGGGA 
CATTTAAGTG TGATTCTGGA CATGTTGGTA ACAACCTGCG GCCAGTACGT GATAACTTGC 
GACCGAGACG AGAAGATCCG CGTGTCCCGT TATCCGAACT CGTATAACAT TGCCACTTTC 
TGCTTGGGGC ATACGGAGTT TGTTACTTCC CTGGCTTTGC TAACTAATCC CAACGTCCTC 
CTGTCGGCTT CGGGGGATGG GACTGTTCGG ATGTGGCACT ACTCGGAGGG CAAACAAATT 
AGCATAATAA ACACGGCTGG TTTGGTGGAT GGGCTCACTA TGGAAAAGTT CGGCAGCCAA 
ATGGATCTGG AAAAAGTGGA CGTAACTGCC CTCCCAGTAA CCAGCATGAA AGTGAGCGAA 
CAAAACGGAC TGGTAACTCT AGCTATTTCC TTATACAGTA TTCCCAAACT GTATCTATAC 
AGCATTAAGA CGCCCAGTTT AGAACATTCG CTTAAGCATA CTGTGGATTT GTATGCCAAC 
ATTGTCTCTT ATTGGCTAAC AAGTCGGCTC TATGTGCTCT CAGATAAGTT TGAGGTATAT 
CAAGTTGACG CTGATACTCT TAGGGCAGTG GACGCGAAAA CTACCCAAAA TAGACTAGTT 
GAGTATAAAG CGGCCTTTGT AAGTAACGAC TGCGACGTTA CAGCGTGGTA TAAAAGGAAG 
TTTGATAACG TTCAGGAATA CATGGAGCGA AAAAAGCTGA GGCTGGAGTC GAAATAA