ORF » Species Summary » Danio rerio » dhfr cDNA ORF clone

dhfr cDNA ORF clone, Danio rerio(zebrafish)

The following dhfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dhfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa32470 NM_131775.1
Latest version!		 Danio rerio dihydrofolate reductase (dhfr), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    dhfr ( NM_131775.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571850.1 Danio rerio dihydrofolate reductase (dhfr), mRNA.
    Price & Availability↑
    CloneID ODa32470
    Clone ID Related Accession (Same CDS sequence) NM_131775.1
    Accession Version NM_131775.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-09-01
    Organism Danio rerio(zebrafish)
    Product dihydrofolate reductase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC071330.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071330.1, EV760294.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGTCTCGAA TACTCAACTG TATTGTAGCT GTTTGTCCAG ACATGGGAAT AGGGAAAAAT 
    GGAAACCTGC CATGGCATCC AATAAGACTA AGTAATGAAC TCAAGCACTT TCAGAAGATG 
    ACCATGACCC CTTCAGATGA GGGTAAGAAG AATGTGGTCA TCATGGGCAG AAAGACATGG 
    TTCTCTATTC CTGCAGCGCA CAGACCTCTA AAGAACAGGA TCAACATAGT TCTCAGCAGA 
    GAACTCAAGA CAGCCCCAGA GGGAGCTCAC TACCTTGCTT CAGACTTCAG TTCAGCTCTC 
    CATCTGTTAG ACAGTGGCGA GCTCGAAAAA CTAGTGGACC AGGTTTGGAT AATTGGTGGC 
    AGCTCTTTGT ATAAGGAGGT GATGGAGCGC TCTGGTCACA GGCGTCTGTT TGTAACCCGC 
    ATCCTCAAGC AGTTTGACTG TGACACATTT ATACCCAATT TTGACATGGA CAAGTACAAG 
    CTACTGCCTG AATTTCCAGG TGTACCCGTT GGCTTGCAGG AGGACAATGG TGTTCAGTAT 
    TTGTTTGAAG TGTATGAAAG CATCAAACAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_571850.1
    CDS84..656
    Translation

    Target ORF information:

    RefSeq Version NM_131775.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio dihydrofolate reductase (dhfr), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_131775.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGTCTCGAA TACTCAACTG TATTGTAGCT GTTTGTCCAG ACATGGGAAT AGGGAAAAAT 
    GGAAACCTGC CATGGCATCC AATAAGACTA AGTAATGAAC TCAAGCACTT TCAGAAGATG 
    ACCATGACCC CTTCAGATGA GGGTAAGAAG AATGTGGTCA TCATGGGCAG AAAGACATGG 
    TTCTCTATTC CTGCAGCGCA CAGACCTCTA AAGAACAGGA TCAACATAGT TCTCAGCAGA 
    GAACTCAAGA CAGCCCCAGA GGGAGCTCAC TACCTTGCTT CAGACTTCAG TTCAGCTCTC 
    CATCTGTTAG ACAGTGGCGA GCTCGAAAAA CTAGTGGACC AGGTTTGGAT AATTGGTGGC 
    AGCTCTTTGT ATAAGGAGGT GATGGAGCGC TCTGGTCACA GGCGTCTGTT TGTAACCCGC 
    ATCCTCAAGC AGTTTGACTG TGACACATTT ATACCCAATT TTGACATGGA CAAGTACAAG 
    CTACTGCCTG AATTTCCAGG TGTACCCGTT GGCTTGCAGG AGGACAATGG TGTTCAGTAT 
    TTGTTTGAAG TGTATGAAAG CATCAAACAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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