chrnb3a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chrnb3a
Entrez Gene ID	794401
Full Name	cholinergic receptor, nicotinic, beta polypeptide 3a
General protein information	Preferred Names cholinergic receptor, nicotinic, beta polypeptide 3a Names cholinergic receptor, nicotinic, beta polypeptide 3a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201220.1	NP_957514.1	cholinergic receptor, nicotinic, beta polypeptide 3a

Pathways from BioSystems

Homologies

	CHRNB3	XP_001115605.1
Bos taurus (cattle)	CHRNB3	XP_002698773.2
Rattus norvegicus (Norway rat)	Chrnb3	NP_598281.1
Gallus gallus (chicken)	CHRNB3	NP_990143.1
Danio rerio (zebrafish)	chrnb3b	NP_775394.1
Xenopus tropicalis (tropical clawed frog)	chrnb3	XP_002942943.1
Mus musculus (house mouse)	Chrnb3	NP_775304.1
Danio rerio (zebrafish)	chrnb3a	NP_957514.1
Homo sapiens (human)	CHRNB3	NP_000740.1
Pan troglodytes (chimpanzee)	CHRNB3	NP_001029105.1
Canis lupus familiaris (dog)	CHRNB3	XP_539952.3

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
Deng C, Chen H, Yang N, Feng Y, Hsueh AJ
The Journal of biological chemistry290(30)18261-8(2015 Jul)

Activation of ?2A-containing nicotinic acetylcholine receptors mediates nicotine-induced motor output in embryonic zebrafish.
Menelaou E, Udvadia AJ, Tanguay RL, Svoboda KR
The European journal of neuroscience40(1)2225-40(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chrnb3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chrnb3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa05229	NM_201220.1 Latest version!	Danio rerio cholinergic receptor, nicotinic, beta polypeptide 3a (chrnb3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chrnb3a ( NM_201220.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957514.1 Danio rerio cholinergic receptor, nicotinic, beta polypeptide 3a (chrnb3a), mRNA.

Price & Availability↑

CloneID	ODa05229
Clone ID Related Accession (Same CDS sequence)	NM_201220.1
Accession Version	NM_201220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	cholinergic receptor, nicotinic, beta polypeptide 3a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY256909.1. On Mar 1, 2008 this sequence version replaced XM_001333594.1. ##Evidence-Data-START## Transcript exon combination :: AY256909.1, BC162133.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAGTTAC AAATCTCCGG TATTCTGCTC GTAGCGGCAG CAGCCTATGC AACAATAGAG 
GCTCCCGAGG AGTTTGTCTC ATTGGCTGAA ATGGAGGACA CTTTATTGAG GAATCTTTTT 
CGGGGATATC AGAAGTGGGT GAGACCTGTC CTTCACGCCA ACGACACGAT TACTGTTCGC 
TTCGGTCTGA AGATCTCACA GTTGGTGGAT GTGGATGAAA AAAATCAACT GATGACCACA 
AATGTCTGGC TGTGGGAGGA GTGGACAGAC TACAAACTGC GCTGGAATCC AGAAGACTAT 
GGGGGAATCA CATCTATCAG AGTGCCCTCA GAAACAATTT GGCTGCCAGA TATTGTCCTT 
TATGAAAATG CTGATGGCCG ATTTGAGGGA TCTCTAATGA CTAAAGCCAT TGTAAGGTTT 
AACGGTACCA TCATGTGGAC TCCTCCTGCC AGCTATAAAT CCGCCTGCAC CATGGACGTC 
ACCTTTTTCC CCTTTGACAG ACAGAACTGC TCAATGAAGT TTGGATCATG GACATACGAT 
GGCACTATGG TGGATCTGAC CCTTGTTGAC GCTTACGTGG ACCGTAAAGA CTTCTTTGAC 
AACGGCGAAT GGGAAATACT CAATGCCACC GGCCAAAGAG GCAGTCGACG TGACGGCATT 
TACTCTTATC CTTACCTCAC ATATTCATTC ATCCTTAAGC GTCTCCCTCT GTTTTACACA 
CTCTTTCTCA TCATCCCGTG TCTCGGTTTG TCGTTTCTCA CTGTGCTGGT CTTCTACCTT 
CCATCAGACG AAGGGGAAAA GCTTTCCCTC TCCACTTCCG TTCTTGTGTC TCTCACTGTG 
TTTCTTCTCG TCATTGAAGA GATCATTCCG TCATCCTCTA AAGTCATTCC TCTGATTGGA 
GAGTATCTGC TTTTTATCAT GATCTTTGTC ACTTTCTCGA TCATCGTAAC TGTCTTTGTC 
ATCAACGTTC ATCATCGTTC CTCTGCCACT TACCATCCCA TGGCACCTTG GGTGAAGAGT 
CTTTTCCTCC AGAGATTACC CAGACTGCTG TGCATGAGAG GACACACTGA CCGATATCAG 
TACCCGGACA TCGAGCTGCG GAGCCCAGAG CTCAAACGAG GGATGAAGAA AGGGCAACAG 
AAGAGTGCTG GAGGAGGACG AGCTGGAGTT AAGGAGGATG AAAACCAGGC CTGGATTGCC 
CTGTTGGAGA AAGCCACCCA CTCAGTGCAC TACATTTCCA GACACATCCG GAAGGAACAC 
TTCATCAGAG AGGTTGTTCA GGATTGGAAA TTTGTGGCTC AGGTGTTGGA CCGGATTTTC 
CTGTGGGCCT TTCTCACAGT TTCTGTGCTT GGGACCATCC TCATCTTCAC CCCAGCTTTG 
CAGATGTTTT TTAGCACATC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957514.1
CDS	251..1654
Translation	MKLQISGILLVAAAAYATIEAPEEFVSLAEMEDTLLRNLFRGYQKWVRPVLHANDTITVRFGLKISQLVDVDEKNQLMTTNVWLWEEWTDYKLRWNPEDYGGITSIRVPSETIWLPDIVLYENADGRFEGSLMTKAIVRFNGTIMWTPPASYKSACTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLTLVDAYVDRKDFFDNGEWEILNATGQRGSRRDGIYSYPYLTYSFILKRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTFSIIVTVFVINVHHRSSATYHPMAPWVKSLFLQRLPRLLCMRGHTDRYQYPDIELRSPELKRGMKKGQQKSAGGGRAGVKEDENQAWIALLEKATHSVHYISRHIRKEHFIREVVQDWKFVAQVLDRIFLWAFLTVSVLGTILIFTPALQMFFSTS

Target ORF information:

RefSeq Version	NM_201220.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cholinergic receptor, nicotinic, beta polypeptide 3a (chrnb3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201220.1

ORF Insert Sequence:

ATGAAGTTAC AAATCTCCGG TATTCTGCTC GTAGCGGCAG CAGCCTATGC AACAATAGAG 
GCTCCCGAGG AGTTTGTCTC ATTGGCTGAA ATGGAGGACA CTTTATTGAG GAATCTTTTT 
CGGGGATATC AGAAGTGGGT GAGACCTGTC CTTCACGCCA ACGACACGAT TACTGTTCGC 
TTCGGTCTGA AGATCTCACA GTTGGTGGAT GTGGATGAAA AAAATCAACT GATGACCACA 
AATGTCTGGC TGTGGGAGGA GTGGACAGAC TACAAACTGC GCTGGAATCC AGAAGACTAT 
GGGGGAATCA CATCTATCAG AGTGCCCTCA GAAACAATTT GGCTGCCAGA TATTGTCCTT 
TATGAAAATG CTGATGGCCG ATTTGAGGGA TCTCTAATGA CTAAAGCCAT TGTAAGGTTT 
AACGGTACCA TCATGTGGAC TCCTCCTGCC AGCTATAAAT CCGCCTGCAC CATGGACGTC 
ACCTTTTTCC CCTTTGACAG ACAGAACTGC TCAATGAAGT TTGGATCATG GACATACGAT 
GGCACTATGG TGGATCTGAC CCTTGTTGAC GCTTACGTGG ACCGTAAAGA CTTCTTTGAC 
AACGGCGAAT GGGAAATACT CAATGCCACC GGCCAAAGAG GCAGTCGACG TGACGGCATT 
TACTCTTATC CTTACCTCAC ATATTCATTC ATCCTTAAGC GTCTCCCTCT GTTTTACACA 
CTCTTTCTCA TCATCCCGTG TCTCGGTTTG TCGTTTCTCA CTGTGCTGGT CTTCTACCTT 
CCATCAGACG AAGGGGAAAA GCTTTCCCTC TCCACTTCCG TTCTTGTGTC TCTCACTGTG 
TTTCTTCTCG TCATTGAAGA GATCATTCCG TCATCCTCTA AAGTCATTCC TCTGATTGGA 
GAGTATCTGC TTTTTATCAT GATCTTTGTC ACTTTCTCGA TCATCGTAAC TGTCTTTGTC 
ATCAACGTTC ATCATCGTTC CTCTGCCACT TACCATCCCA TGGCACCTTG GGTGAAGAGT 
CTTTTCCTCC AGAGATTACC CAGACTGCTG TGCATGAGAG GACACACTGA CCGATATCAG 
TACCCGGACA TCGAGCTGCG GAGCCCAGAG CTCAAACGAG GGATGAAGAA AGGGCAACAG 
AAGAGTGCTG GAGGAGGACG AGCTGGAGTT AAGGAGGATG AAAACCAGGC CTGGATTGCC 
CTGTTGGAGA AAGCCACCCA CTCAGTGCAC TACATTTCCA GACACATCCG GAAGGAACAC 
TTCATCAGAG AGGTTGTTCA GGATTGGAAA TTTGTGGCTC AGGTGTTGGA CCGGATTTTC 
CTGTGGGCCT TTCTCACAGT TTCTGTGCTT GGGACCATCC TCATCTTCAC CCCAGCTTTG 
CAGATGTTTT TTAGCACATC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
The Journal of biological chemistry290(30)18261-8(2015 Jul)
Deng C,Chen H,Yang N,Feng Y,Hsueh AJ

Activation of ?2A-containing nicotinic acetylcholine receptors mediates nicotine-induced motor output in embryonic zebrafish.
The European journal of neuroscience40(1)2225-40(2014 Jul)
Menelaou E,Udvadia AJ,Tanguay RL,Svoboda KR