git2a cDNA ORF clone, Danio rerio(zebrafish)

The following git2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the git2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa142701 XM_021476321.1
Latest version!
Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
ODa02666 NM_001126453.1
Latest version!
Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa10302 XM_005165184.4
Latest version!
Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa10303 XM_005165185.4
Latest version!
Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa46849 XM_009301357.3
Latest version!
Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa46851 XM_009301359.3
Latest version!
Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa142700 XM_021476320.1
Latest version!
Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
ODa10302 XM_005165184.3 Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa10303 XM_005165185.3 Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa46849 XM_009301357.2 Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa46851 XM_009301359.2 Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa142701
    Clone ID Related Accession (Same CDS sequence) XM_021476321.1
    Accession Version XM_021476321.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ARF GTPase-activating protein GIT2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAGT GAACAACAGA AAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331996.1
    CDS590..1888
    Translation

    Target ORF information:

    RefSeq Version XM_021476321.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476321.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAGT GAACAACAGA AAACTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa02666
    Clone ID Related Accession (Same CDS sequence) NM_001126453.1
    Accession Version NM_001126453.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product ARF GTPase-activating protein GIT2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX323811.14. On Jul 31, 2008 this sequence version replaced XM_001332664.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGCAGTGT GGAGTTAACA GAGTTTGCAA AAGCTGCAAA AAGAAAACTA
    CAGTCTCTCA GCGATCACTT GTTTGAGGAA TTGGCAATGG ATGTTTATGA TGAAGTAGAC
    CGACGAGAGA CAGATGCAGT TTGGCTGGCC ACTCAGAACC ACAGCACCTT GGTAACAGAG
    ACAACAGTTG TGCCTTTCCT TCCTGTCAAC CCTGAGTACT CATCAACACG AAACCAGGGA
    AGACAAAAGC TGGCAAGATT CAACGCTCAT GAATTTGCCA CACTTGTGAT CGATATTCTA
    AGTGATGCAA AGCGACGCCA GCATGGAAAT CCAGTCCCAA GTCCTAAAGA CAATGTTGAT
    GTATTCTTCA AAAGTGGGGG CAGTCGTCAT GGAAGTGAAA GTCAGGAAAT TGACCAGCCA
    GATTATGACA GCGTGGCGTC CGATGAGGAC ACCGATACTG ATTTACGAAT GGGCAAAGCA
    GACAGAACTA AAAGTCTGGA CTCGGATCTC TCCGATGGAC CGGTTTCCGT GCAGGAGTTT
    CTGGAGGTGA AAAACGCACT CTCAGCCTCG GAGGCCAAAA TTCAGGAGCT GATGAAGGCC
    AACAGTAATC TGAATGAAGA GCTGAGATTA ATGCAGAAAA AGCTGCAGTC TCTGCAAAGC
    GAGAACAGCT CTCTGAGGCG GCAGGTCTCC GCTCAACAGC CCTATCAGGC CCCCGGCGGC
    CCTGACCATC CCAATCCCTC CAGTCCCTCC TCCTGTTCCT CCTCCTCCTC TTCCTCCTCT
    GCCCTGAAAC GCCACCTGTC TGCGCGCGCC AGTCGCCCCA TGTCTATGTA TGAAACCGGC
    TCTGGCCTGA AGCCCTTTCT TCCCAAGGGC GAGACCCCTT ACCCTGAGGA AACCTTCCCC
    ACCCTGCAAC CCTTCCCAGC CTATGCCTCC AAATTTGACA AGCAGAGCAG CGTGTCTGAT
    GGCGACTACG ACAACACCGT CAACGAATCT GATCTGGACG ACTCTGGTTT TGGCCGGCGA
    TGTCGCCTTC GCAGCAGTGG CTGGATGGGA GAAAGCGGCT CTATCCCCGA GCTGGAGGAC
    CATGAGTGTG AGCTGGACCC CAGTCTGCCC AGCACCGAAG ACGTCATACG CAAAACTGAG
    CAGATCACCA AGAACATTCA GGAGCTGCTG AGGGCCGCGC AGGAGAACAA GCACGAGAGC
    TTTGTACCCT GCGCAGAAAG AATACATGTG GCTGTGAATG AGATGGCGGC TCTGTTCCCC
    AAGAGACCAC GTTCTGAGAC GGTCAGGAAC TCTCTGCGGT TATTAACGTC CAGTGCGAAT
    CGGCTCCAGA CCGAGTGTAA GAAAGCGTCT CCTCTGGAGA GCAGCCAGGC CTCAGACATG
    CAGTTGGTCA CTCAGCAGGT CATCCAGTGT GCTTACGACA TCGCCAAGGC TGCAAAGCAA
    CTAGTTACTA TAACCACCAA AGAGAACAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001119925.1
    CDS311..2563
    Translation

    Target ORF information:

    RefSeq Version NM_001126453.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001126453.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGCAGTGT GGAGTTAACA GAGTTTGCAA AAGCTGCAAA AAGAAAACTA
    CAGTCTCTCA GCGATCACTT GTTTGAGGAA TTGGCAATGG ATGTTTATGA TGAAGTAGAC
    CGACGAGAGA CAGATGCAGT TTGGCTGGCC ACTCAGAACC ACAGCACCTT GGTAACAGAG
    ACAACAGTTG TGCCTTTCCT TCCTGTCAAC CCTGAGTACT CATCAACACG AAACCAGGGA
    AGACAAAAGC TGGCAAGATT CAACGCTCAT GAATTTGCCA CACTTGTGAT CGATATTCTA
    AGTGATGCAA AGCGACGCCA GCATGGAAAT CCAGTCCCAA GTCCTAAAGA CAATGTTGAT
    GTATTCTTCA AAAGTGGGGG CAGTCGTCAT GGAAGTGAAA GTCAGGAAAT TGACCAGCCA
    GATTATGACA GCGTGGCGTC CGATGAGGAC ACCGATACTG ATTTACGAAT GGGCAAAGCA
    GACAGAACTA AAAGTCTGGA CTCGGATCTC TCCGATGGAC CGGTTTCCGT GCAGGAGTTT
    CTGGAGGTGA AAAACGCACT CTCAGCCTCG GAGGCCAAAA TTCAGGAGCT GATGAAGGCC
    AACAGTAATC TGAATGAAGA GCTGAGATTA ATGCAGAAAA AGCTGCAGTC TCTGCAAAGC
    GAGAACAGCT CTCTGAGGCG GCAGGTCTCC GCTCAACAGC CCTATCAGGC CCCCGGCGGC
    CCTGACCATC CCAATCCCTC CAGTCCCTCC TCCTGTTCCT CCTCCTCCTC TTCCTCCTCT
    GCCCTGAAAC GCCACCTGTC TGCGCGCGCC AGTCGCCCCA TGTCTATGTA TGAAACCGGC
    TCTGGCCTGA AGCCCTTTCT TCCCAAGGGC GAGACCCCTT ACCCTGAGGA AACCTTCCCC
    ACCCTGCAAC CCTTCCCAGC CTATGCCTCC AAATTTGACA AGCAGAGCAG CGTGTCTGAT
    GGCGACTACG ACAACACCGT CAACGAATCT GATCTGGACG ACTCTGGTTT TGGCCGGCGA
    TGTCGCCTTC GCAGCAGTGG CTGGATGGGA GAAAGCGGCT CTATCCCCGA GCTGGAGGAC
    CATGAGTGTG AGCTGGACCC CAGTCTGCCC AGCACCGAAG ACGTCATACG CAAAACTGAG
    CAGATCACCA AGAACATTCA GGAGCTGCTG AGGGCCGCGC AGGAGAACAA GCACGAGAGC
    TTTGTACCCT GCGCAGAAAG AATACATGTG GCTGTGAATG AGATGGCGGC TCTGTTCCCC
    AAGAGACCAC GTTCTGAGAC GGTCAGGAAC TCTCTGCGGT TATTAACGTC CAGTGCGAAT
    CGGCTCCAGA CCGAGTGTAA GAAAGCGTCT CCTCTGGAGA GCAGCCAGGC CTCAGACATG
    CAGTTGGTCA CTCAGCAGGT CATCCAGTGT GCTTACGACA TCGCCAAGGC TGCAAAGCAA
    CTAGTTACTA TAACCACCAA AGAGAACAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10302
    Clone ID Related Accession (Same CDS sequence) XM_005165184.3 , XM_005165184.4
    Accession Version XM_005165184.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ARF GTPase-activating protein GIT2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165184.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGCT GCAGTCTCTG
    CAAAGCGAGA ACAGCTCTCT GAGGCGGCAG GTCTCCGCTC AACAGCCCTA TCAGGCCCCC
    GGCGGCCCTG ACCATCCCAA TCCCTCCAGT CCCTCCTCCT GTTCCTCCTC CTCCTCTTCC
    TCCTCTGCCC TGAAACGCCA CCTGTCTGCG CGCGCCAGTC GCCCCATGTC TATGTATGAA
    ACCGGCTCTG GCCTGAAGCC CTTTCTTCCC AAGGGCGAGA CCCCTTACCC TGAGGAAACC
    TTCCCCACCC TGCAACCCTT CCCAGCCTAT GCCTCCAAAT TTGACAAGCA GAGCAGCGTG
    TCTGATGGCG ACTACGACAA CACCGTCAAC GAATCTGATC TGGACGACTC TGGTTTTGGC
    CGGCGATGTC GCCTTCGCAG CAGTGGCTGG ATGGGAGAAA GCGGCTCTAT CCCCGAGCTG
    GAGGACCATG AGTGTGAGCT GGACCCCAGT CTGCCCAGCA CCGAAGACGT CATACGCAAA
    ACTGAGCAGA TCACCAAGAA CATTCAGGAG CTGCTGAGGG CCGCGCAGGA GAACAAGCAC
    GAGAGTGCAC CATGTGAGCA GAGGGAGAGT GTTCGCAGAC TTAGACACAG TCTGGGTTGT
    TTCAGCACAC TGGTGCCTTG GGCAGACAAA CCCCCGTCTC CCATGCAGCC CCTTGGCCTC
    AGAGCTCCCC ATCCAGCCAA CCCCAACAAC CCTGCCTCCT GCTTTGTACC CTGCGCAGAA
    AGAATACATG TGGCTGTGAA TGAGATGGCG GCTCTGTTCC CCAAGAGACC ACGTTCTGAG
    ACGGTCAGGA ACTCTCTGCG GTTATTAACG TCCAGTGCGA ATCGGCTCCA GACCGAGTGT
    AAGAAAGCGT CTCCTCTGGA GAGCAGCCAG GCCTCAGACA TGCAGTTGGT CACTCAGCAG
    GTCATCCAGT GTGCTTACGA CATCGCCAAG GCTGCAAAGC AACTAGTTAC TATAACCACC
    AAAGAGAACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165241.1
    CDS589..3003
    Translation

    Target ORF information:

    RefSeq Version XM_005165184.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165184.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGCT GCAGTCTCTG
    CAAAGCGAGA ACAGCTCTCT GAGGCGGCAG GTCTCCGCTC AACAGCCCTA TCAGGCCCCC
    GGCGGCCCTG ACCATCCCAA TCCCTCCAGT CCCTCCTCCT GTTCCTCCTC CTCCTCTTCC
    TCCTCTGCCC TGAAACGCCA CCTGTCTGCG CGCGCCAGTC GCCCCATGTC TATGTATGAA
    ACCGGCTCTG GCCTGAAGCC CTTTCTTCCC AAGGGCGAGA CCCCTTACCC TGAGGAAACC
    TTCCCCACCC TGCAACCCTT CCCAGCCTAT GCCTCCAAAT TTGACAAGCA GAGCAGCGTG
    TCTGATGGCG ACTACGACAA CACCGTCAAC GAATCTGATC TGGACGACTC TGGTTTTGGC
    CGGCGATGTC GCCTTCGCAG CAGTGGCTGG ATGGGAGAAA GCGGCTCTAT CCCCGAGCTG
    GAGGACCATG AGTGTGAGCT GGACCCCAGT CTGCCCAGCA CCGAAGACGT CATACGCAAA
    ACTGAGCAGA TCACCAAGAA CATTCAGGAG CTGCTGAGGG CCGCGCAGGA GAACAAGCAC
    GAGAGTGCAC CATGTGAGCA GAGGGAGAGT GTTCGCAGAC TTAGACACAG TCTGGGTTGT
    TTCAGCACAC TGGTGCCTTG GGCAGACAAA CCCCCGTCTC CCATGCAGCC CCTTGGCCTC
    AGAGCTCCCC ATCCAGCCAA CCCCAACAAC CCTGCCTCCT GCTTTGTACC CTGCGCAGAA
    AGAATACATG TGGCTGTGAA TGAGATGGCG GCTCTGTTCC CCAAGAGACC ACGTTCTGAG
    ACGGTCAGGA ACTCTCTGCG GTTATTAACG TCCAGTGCGA ATCGGCTCCA GACCGAGTGT
    AAGAAAGCGT CTCCTCTGGA GAGCAGCCAG GCCTCAGACA TGCAGTTGGT CACTCAGCAG
    GTCATCCAGT GTGCTTACGA CATCGCCAAG GCTGCAAAGC AACTAGTTAC TATAACCACC
    AAAGAGAACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10302
    Clone ID Related Accession (Same CDS sequence) XM_005165184.3 , XM_005165184.4
    Accession Version XM_005165184.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ARF GTPase-activating protein GIT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165184.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGCT GCAGTCTCTG
    CAAAGCGAGA ACAGCTCTCT GAGGCGGCAG GTCTCCGCTC AACAGCCCTA TCAGGCCCCC
    GGCGGCCCTG ACCATCCCAA TCCCTCCAGT CCCTCCTCCT GTTCCTCCTC CTCCTCTTCC
    TCCTCTGCCC TGAAACGCCA CCTGTCTGCG CGCGCCAGTC GCCCCATGTC TATGTATGAA
    ACCGGCTCTG GCCTGAAGCC CTTTCTTCCC AAGGGCGAGA CCCCTTACCC TGAGGAAACC
    TTCCCCACCC TGCAACCCTT CCCAGCCTAT GCCTCCAAAT TTGACAAGCA GAGCAGCGTG
    TCTGATGGCG ACTACGACAA CACCGTCAAC GAATCTGATC TGGACGACTC TGGTTTTGGC
    CGGCGATGTC GCCTTCGCAG CAGTGGCTGG ATGGGAGAAA GCGGCTCTAT CCCCGAGCTG
    GAGGACCATG AGTGTGAGCT GGACCCCAGT CTGCCCAGCA CCGAAGACGT CATACGCAAA
    ACTGAGCAGA TCACCAAGAA CATTCAGGAG CTGCTGAGGG CCGCGCAGGA GAACAAGCAC
    GAGAGTGCAC CATGTGAGCA GAGGGAGAGT GTTCGCAGAC TTAGACACAG TCTGGGTTGT
    TTCAGCACAC TGGTGCCTTG GGCAGACAAA CCCCCGTCTC CCATGCAGCC CCTTGGCCTC
    AGAGCTCCCC ATCCAGCCAA CCCCAACAAC CCTGCCTCCT GCTTTGTACC CTGCGCAGAA
    AGAATACATG TGGCTGTGAA TGAGATGGCG GCTCTGTTCC CCAAGAGACC ACGTTCTGAG
    ACGGTCAGGA ACTCTCTGCG GTTATTAACG TCCAGTGCGA ATCGGCTCCA GACCGAGTGT
    AAGAAAGCGT CTCCTCTGGA GAGCAGCCAG GCCTCAGACA TGCAGTTGGT CACTCAGCAG
    GTCATCCAGT GTGCTTACGA CATCGCCAAG GCTGCAAAGC AACTAGTTAC TATAACCACC
    AAAGAGAACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165241.1
    CDS590..3004
    Translation

    Target ORF information:

    RefSeq Version XM_005165184.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165184.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGCT GCAGTCTCTG
    CAAAGCGAGA ACAGCTCTCT GAGGCGGCAG GTCTCCGCTC AACAGCCCTA TCAGGCCCCC
    GGCGGCCCTG ACCATCCCAA TCCCTCCAGT CCCTCCTCCT GTTCCTCCTC CTCCTCTTCC
    TCCTCTGCCC TGAAACGCCA CCTGTCTGCG CGCGCCAGTC GCCCCATGTC TATGTATGAA
    ACCGGCTCTG GCCTGAAGCC CTTTCTTCCC AAGGGCGAGA CCCCTTACCC TGAGGAAACC
    TTCCCCACCC TGCAACCCTT CCCAGCCTAT GCCTCCAAAT TTGACAAGCA GAGCAGCGTG
    TCTGATGGCG ACTACGACAA CACCGTCAAC GAATCTGATC TGGACGACTC TGGTTTTGGC
    CGGCGATGTC GCCTTCGCAG CAGTGGCTGG ATGGGAGAAA GCGGCTCTAT CCCCGAGCTG
    GAGGACCATG AGTGTGAGCT GGACCCCAGT CTGCCCAGCA CCGAAGACGT CATACGCAAA
    ACTGAGCAGA TCACCAAGAA CATTCAGGAG CTGCTGAGGG CCGCGCAGGA GAACAAGCAC
    GAGAGTGCAC CATGTGAGCA GAGGGAGAGT GTTCGCAGAC TTAGACACAG TCTGGGTTGT
    TTCAGCACAC TGGTGCCTTG GGCAGACAAA CCCCCGTCTC CCATGCAGCC CCTTGGCCTC
    AGAGCTCCCC ATCCAGCCAA CCCCAACAAC CCTGCCTCCT GCTTTGTACC CTGCGCAGAA
    AGAATACATG TGGCTGTGAA TGAGATGGCG GCTCTGTTCC CCAAGAGACC ACGTTCTGAG
    ACGGTCAGGA ACTCTCTGCG GTTATTAACG TCCAGTGCGA ATCGGCTCCA GACCGAGTGT
    AAGAAAGCGT CTCCTCTGGA GAGCAGCCAG GCCTCAGACA TGCAGTTGGT CACTCAGCAG
    GTCATCCAGT GTGCTTACGA CATCGCCAAG GCTGCAAAGC AACTAGTTAC TATAACCACC
    AAAGAGAACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10303
    Clone ID Related Accession (Same CDS sequence) XM_005165185.3 , XM_005165185.4
    Accession Version XM_005165185.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ARF GTPase-activating protein GIT2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165185.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGCAGTGT GGAGTTAACA GAGTTTGCAA AAGCTGCAAA AAGAAAACTA
    CAGTCTCTCA GCGATCACTT GTTTGAGGAA TTGGCAATGG ATGTTTATGA TGAAGTAGAC
    CGACGAGAGA CAGATGCAGT TTGGCTGGCC ACTCAGAACC ACAGCACCTT GGTAACAGAG
    ACAACAGTTG TGCCTTTCCT TCCTGTCAAC CCTGAGTACT CATCAACACG AAACCAGGGA
    AGACAAAAGC TGGCAAGATT CAACGCTCAT GAATTTGCCA CACTTGTGAT CGATATTCTA
    AGTGATGCAA AGCGACGCCA GCATGGAAAT CCAGTCCCAA GTCCTAAAGA CAATGTTGAT
    GTATTCTTCA AAAGTGGGGG CAGTCGTCAT GGAAGTGAAA GTCAGGAAAT TGACCAGCCA
    GATTATGACA GCGTGGCGTC CGATGAGGAC ACCGATACTG ATTTACGAAT GGGCAAAGCA
    GACAGAACTA AAAGTCTGGA CTCGGATCTC TCCGATGGAC CGGTTTCCGT GCAGGAGTTT
    CTGGAGGTGA AAAACGCACT CTCAGCCTCG GAGGCCAAAA TTCAGGAGCT GATGAAGGCC
    AACAGTAATC TGAATGAAGA GCTGAGATTA ATGCAGAAAA AGCTGCAGTC TCTGCAAAGC
    GAGAACAGCT CTCTGAGGCG GCAGGTCTCC GCTCAACAGC CCTATCAGGC CCCCGGCGGC
    CCTGACCATC CCAATCCCTC CAGTCCCTCC TCCTGTTCCT CCTCCTCCTC TTCCTCCTCT
    GCCCTGAAAC GCCACCTGTC TGCGCGCGCC AGTCGCCCCA TGTCTATGTA TGAAACCGGC
    TCTGGCCTGA AGCCCTTTCT TCCCAAGGGC GAGACCCCTT ACCCTGAGGA AACCTTCCCC
    ACCCTGCAAC CCTTCCCAGC CTATGCCTCC AAATTTGACA AGCAGAGCAG CGTGTCTGAT
    GGCGACTACG ACAACACCGT CAACGAATCT GATCTGGACG ACTCTGGTTT TGGCCGGCGA
    TGTCGCCTTC GCAGCAGTGG CTGGATGGGA GAAAGCGGCT CTATCCCCGA GCTGGAGGAC
    CATGAGTGTG AGCTGGACCC CAGTCTGCCC AGCACCGAAG ACGTCATACG CAAAACTGAG
    CAGATCACCA AGAACATTCA GGAGCTGCTG AGGGCCGCGC AGGAGAACAA GCACGAGAGT
    GCACCATGTG AGCAGAGGGA GAGTGTTCGC AGACTTAGAC ACAGTCTGGG TTGTTTCAGC
    ACACTGGTGC CTTGGGCAGA CAAACCCCCG TCTCCCATGC AGCCCCTTGG CCTCAGAGCT
    CCCCATCCAG CCAACCCCAA CAACCCTGCC TCCTGCTTTG TACCCTGCGC AGAAAGAATA
    CATGTGGCTG TGAATGAGAT GGCGGCTCTG TTCCCCAAGA GACCACGTTC TGAGACGGTC
    AGGAACTCTC TGCGGTTATT AACGTCCAGT GCGAATCGGC TCCAGACCGA GTGTAAGAAA
    GCGTCTCCTC TGGAGAGCAG CCAGGCCTCA GACATGCAGT TGGTCACTCA GCAGGTCATC
    CAGTGTGCTT ACGACATCGC CAAGGCTGCA AAGCAACTAG TTACTATAAC CACCAAAGAG
    AACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165242.1
    CDS589..2997
    Translation

    Target ORF information:

    RefSeq Version XM_005165185.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165185.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGCAGTGT GGAGTTAACA GAGTTTGCAA AAGCTGCAAA AAGAAAACTA
    CAGTCTCTCA GCGATCACTT GTTTGAGGAA TTGGCAATGG ATGTTTATGA TGAAGTAGAC
    CGACGAGAGA CAGATGCAGT TTGGCTGGCC ACTCAGAACC ACAGCACCTT GGTAACAGAG
    ACAACAGTTG TGCCTTTCCT TCCTGTCAAC CCTGAGTACT CATCAACACG AAACCAGGGA
    AGACAAAAGC TGGCAAGATT CAACGCTCAT GAATTTGCCA CACTTGTGAT CGATATTCTA
    AGTGATGCAA AGCGACGCCA GCATGGAAAT CCAGTCCCAA GTCCTAAAGA CAATGTTGAT
    GTATTCTTCA AAAGTGGGGG CAGTCGTCAT GGAAGTGAAA GTCAGGAAAT TGACCAGCCA
    GATTATGACA GCGTGGCGTC CGATGAGGAC ACCGATACTG ATTTACGAAT GGGCAAAGCA
    GACAGAACTA AAAGTCTGGA CTCGGATCTC TCCGATGGAC CGGTTTCCGT GCAGGAGTTT
    CTGGAGGTGA AAAACGCACT CTCAGCCTCG GAGGCCAAAA TTCAGGAGCT GATGAAGGCC
    AACAGTAATC TGAATGAAGA GCTGAGATTA ATGCAGAAAA AGCTGCAGTC TCTGCAAAGC
    GAGAACAGCT CTCTGAGGCG GCAGGTCTCC GCTCAACAGC CCTATCAGGC CCCCGGCGGC
    CCTGACCATC CCAATCCCTC CAGTCCCTCC TCCTGTTCCT CCTCCTCCTC TTCCTCCTCT
    GCCCTGAAAC GCCACCTGTC TGCGCGCGCC AGTCGCCCCA TGTCTATGTA TGAAACCGGC
    TCTGGCCTGA AGCCCTTTCT TCCCAAGGGC GAGACCCCTT ACCCTGAGGA AACCTTCCCC
    ACCCTGCAAC CCTTCCCAGC CTATGCCTCC AAATTTGACA AGCAGAGCAG CGTGTCTGAT
    GGCGACTACG ACAACACCGT CAACGAATCT GATCTGGACG ACTCTGGTTT TGGCCGGCGA
    TGTCGCCTTC GCAGCAGTGG CTGGATGGGA GAAAGCGGCT CTATCCCCGA GCTGGAGGAC
    CATGAGTGTG AGCTGGACCC CAGTCTGCCC AGCACCGAAG ACGTCATACG CAAAACTGAG
    CAGATCACCA AGAACATTCA GGAGCTGCTG AGGGCCGCGC AGGAGAACAA GCACGAGAGT
    GCACCATGTG AGCAGAGGGA GAGTGTTCGC AGACTTAGAC ACAGTCTGGG TTGTTTCAGC
    ACACTGGTGC CTTGGGCAGA CAAACCCCCG TCTCCCATGC AGCCCCTTGG CCTCAGAGCT
    CCCCATCCAG CCAACCCCAA CAACCCTGCC TCCTGCTTTG TACCCTGCGC AGAAAGAATA
    CATGTGGCTG TGAATGAGAT GGCGGCTCTG TTCCCCAAGA GACCACGTTC TGAGACGGTC
    AGGAACTCTC TGCGGTTATT AACGTCCAGT GCGAATCGGC TCCAGACCGA GTGTAAGAAA
    GCGTCTCCTC TGGAGAGCAG CCAGGCCTCA GACATGCAGT TGGTCACTCA GCAGGTCATC
    CAGTGTGCTT ACGACATCGC CAAGGCTGCA AAGCAACTAG TTACTATAAC CACCAAAGAG
    AACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10303
    Clone ID Related Accession (Same CDS sequence) XM_005165185.3 , XM_005165185.4
    Accession Version XM_005165185.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ARF GTPase-activating protein GIT2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165185.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGCAGTGT GGAGTTAACA GAGTTTGCAA AAGCTGCAAA AAGAAAACTA
    CAGTCTCTCA GCGATCACTT GTTTGAGGAA TTGGCAATGG ATGTTTATGA TGAAGTAGAC
    CGACGAGAGA CAGATGCAGT TTGGCTGGCC ACTCAGAACC ACAGCACCTT GGTAACAGAG
    ACAACAGTTG TGCCTTTCCT TCCTGTCAAC CCTGAGTACT CATCAACACG AAACCAGGGA
    AGACAAAAGC TGGCAAGATT CAACGCTCAT GAATTTGCCA CACTTGTGAT CGATATTCTA
    AGTGATGCAA AGCGACGCCA GCATGGAAAT CCAGTCCCAA GTCCTAAAGA CAATGTTGAT
    GTATTCTTCA AAAGTGGGGG CAGTCGTCAT GGAAGTGAAA GTCAGGAAAT TGACCAGCCA
    GATTATGACA GCGTGGCGTC CGATGAGGAC ACCGATACTG ATTTACGAAT GGGCAAAGCA
    GACAGAACTA AAAGTCTGGA CTCGGATCTC TCCGATGGAC CGGTTTCCGT GCAGGAGTTT
    CTGGAGGTGA AAAACGCACT CTCAGCCTCG GAGGCCAAAA TTCAGGAGCT GATGAAGGCC
    AACAGTAATC TGAATGAAGA GCTGAGATTA ATGCAGAAAA AGCTGCAGTC TCTGCAAAGC
    GAGAACAGCT CTCTGAGGCG GCAGGTCTCC GCTCAACAGC CCTATCAGGC CCCCGGCGGC
    CCTGACCATC CCAATCCCTC CAGTCCCTCC TCCTGTTCCT CCTCCTCCTC TTCCTCCTCT
    GCCCTGAAAC GCCACCTGTC TGCGCGCGCC AGTCGCCCCA TGTCTATGTA TGAAACCGGC
    TCTGGCCTGA AGCCCTTTCT TCCCAAGGGC GAGACCCCTT ACCCTGAGGA AACCTTCCCC
    ACCCTGCAAC CCTTCCCAGC CTATGCCTCC AAATTTGACA AGCAGAGCAG CGTGTCTGAT
    GGCGACTACG ACAACACCGT CAACGAATCT GATCTGGACG ACTCTGGTTT TGGCCGGCGA
    TGTCGCCTTC GCAGCAGTGG CTGGATGGGA GAAAGCGGCT CTATCCCCGA GCTGGAGGAC
    CATGAGTGTG AGCTGGACCC CAGTCTGCCC AGCACCGAAG ACGTCATACG CAAAACTGAG
    CAGATCACCA AGAACATTCA GGAGCTGCTG AGGGCCGCGC AGGAGAACAA GCACGAGAGT
    GCACCATGTG AGCAGAGGGA GAGTGTTCGC AGACTTAGAC ACAGTCTGGG TTGTTTCAGC
    ACACTGGTGC CTTGGGCAGA CAAACCCCCG TCTCCCATGC AGCCCCTTGG CCTCAGAGCT
    CCCCATCCAG CCAACCCCAA CAACCCTGCC TCCTGCTTTG TACCCTGCGC AGAAAGAATA
    CATGTGGCTG TGAATGAGAT GGCGGCTCTG TTCCCCAAGA GACCACGTTC TGAGACGGTC
    AGGAACTCTC TGCGGTTATT AACGTCCAGT GCGAATCGGC TCCAGACCGA GTGTAAGAAA
    GCGTCTCCTC TGGAGAGCAG CCAGGCCTCA GACATGCAGT TGGTCACTCA GCAGGTCATC
    CAGTGTGCTT ACGACATCGC CAAGGCTGCA AAGCAACTAG TTACTATAAC CACCAAAGAG
    AACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165242.1
    CDS590..2998
    Translation

    Target ORF information:

    RefSeq Version XM_005165185.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165185.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGCAGTGT GGAGTTAACA GAGTTTGCAA AAGCTGCAAA AAGAAAACTA
    CAGTCTCTCA GCGATCACTT GTTTGAGGAA TTGGCAATGG ATGTTTATGA TGAAGTAGAC
    CGACGAGAGA CAGATGCAGT TTGGCTGGCC ACTCAGAACC ACAGCACCTT GGTAACAGAG
    ACAACAGTTG TGCCTTTCCT TCCTGTCAAC CCTGAGTACT CATCAACACG AAACCAGGGA
    AGACAAAAGC TGGCAAGATT CAACGCTCAT GAATTTGCCA CACTTGTGAT CGATATTCTA
    AGTGATGCAA AGCGACGCCA GCATGGAAAT CCAGTCCCAA GTCCTAAAGA CAATGTTGAT
    GTATTCTTCA AAAGTGGGGG CAGTCGTCAT GGAAGTGAAA GTCAGGAAAT TGACCAGCCA
    GATTATGACA GCGTGGCGTC CGATGAGGAC ACCGATACTG ATTTACGAAT GGGCAAAGCA
    GACAGAACTA AAAGTCTGGA CTCGGATCTC TCCGATGGAC CGGTTTCCGT GCAGGAGTTT
    CTGGAGGTGA AAAACGCACT CTCAGCCTCG GAGGCCAAAA TTCAGGAGCT GATGAAGGCC
    AACAGTAATC TGAATGAAGA GCTGAGATTA ATGCAGAAAA AGCTGCAGTC TCTGCAAAGC
    GAGAACAGCT CTCTGAGGCG GCAGGTCTCC GCTCAACAGC CCTATCAGGC CCCCGGCGGC
    CCTGACCATC CCAATCCCTC CAGTCCCTCC TCCTGTTCCT CCTCCTCCTC TTCCTCCTCT
    GCCCTGAAAC GCCACCTGTC TGCGCGCGCC AGTCGCCCCA TGTCTATGTA TGAAACCGGC
    TCTGGCCTGA AGCCCTTTCT TCCCAAGGGC GAGACCCCTT ACCCTGAGGA AACCTTCCCC
    ACCCTGCAAC CCTTCCCAGC CTATGCCTCC AAATTTGACA AGCAGAGCAG CGTGTCTGAT
    GGCGACTACG ACAACACCGT CAACGAATCT GATCTGGACG ACTCTGGTTT TGGCCGGCGA
    TGTCGCCTTC GCAGCAGTGG CTGGATGGGA GAAAGCGGCT CTATCCCCGA GCTGGAGGAC
    CATGAGTGTG AGCTGGACCC CAGTCTGCCC AGCACCGAAG ACGTCATACG CAAAACTGAG
    CAGATCACCA AGAACATTCA GGAGCTGCTG AGGGCCGCGC AGGAGAACAA GCACGAGAGT
    GCACCATGTG AGCAGAGGGA GAGTGTTCGC AGACTTAGAC ACAGTCTGGG TTGTTTCAGC
    ACACTGGTGC CTTGGGCAGA CAAACCCCCG TCTCCCATGC AGCCCCTTGG CCTCAGAGCT
    CCCCATCCAG CCAACCCCAA CAACCCTGCC TCCTGCTTTG TACCCTGCGC AGAAAGAATA
    CATGTGGCTG TGAATGAGAT GGCGGCTCTG TTCCCCAAGA GACCACGTTC TGAGACGGTC
    AGGAACTCTC TGCGGTTATT AACGTCCAGT GCGAATCGGC TCCAGACCGA GTGTAAGAAA
    GCGTCTCCTC TGGAGAGCAG CCAGGCCTCA GACATGCAGT TGGTCACTCA GCAGGTCATC
    CAGTGTGCTT ACGACATCGC CAAGGCTGCA AAGCAACTAG TTACTATAAC CACCAAAGAG
    AACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46849
    Clone ID Related Accession (Same CDS sequence) XM_009301357.2 , XM_009301357.3
    Accession Version XM_009301357.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ARF GTPase-activating protein GIT2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGCT GCAGTCTCTG
    CAAAGCGAGA ACAGCTCTCT GAGGCGGCAG GTCTCCGCTC AACAGCCCTA TCAGGCCCCC
    GGCGGCCCTG ACCATCCCAA TCCCTCCAGT CCCTCCTCCT GTTCCTCCTC CTCCTCTTCC
    TCCTCTGCCC TGAAACGCCA CCTGTCTGCG CGCGCCAGTC GCCCCATGTC TATGTATGAA
    ACCGGCTCTG GCCTGAAGCC CTTTCTTCCC AAGGGCGAGA CCCCTTACCC TGAGGAAACC
    TTCCCCACCC TGCAACCCTT CCCAGCCTAT GCCTCCAAAT TTGACAAGCA GAGCAGCGTG
    TCTGATGGCG ACTACGACAA CACCGTCAAC GAATCTGATC TGGACGACTC TGGTTTTGGC
    CGGCGATGTC GCCTTCGCAG CAGTGGCTGG ATGGGAGAAA GCGGCTCTAT CCCCGAGCTG
    GAGGACCATG AGTGTGAGCT GGACCCCAGT CTGCCCAGCA CCGAAGACGT CATACGCAAA
    ACTGAGCAGA TCACCAAGAA CATTCAGGAG CTGCTGAGGG CCGCGCAGGA GAACAAGCAC
    GAGAGCTTTG TACCCTGCGC AGAAAGAATA CATGTGGCTG TGAATGAGAT GGCGGCTCTG
    TTCCCCAAGA GACCACGTTC TGAGACGGTC AGGAACTCTC TGCGGTTATT AACGTCCAGT
    GCGAATCGGC TCCAGACCGA GTGTAAGAAA GCGTCTCCTC TGGAGAGCAG CCAGGCCTCA
    GACATGCAGT TGGTCACTCA GCAGGTCATC CAGTGTGCTT ACGACATCGC CAAGGCTGCA
    AAGCAACTAG TTACTATAAC CACCAAAGAG AACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299632.1
    CDS589..2847
    Translation

    Target ORF information:

    RefSeq Version XM_009301357.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301357.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGCT GCAGTCTCTG
    CAAAGCGAGA ACAGCTCTCT GAGGCGGCAG GTCTCCGCTC AACAGCCCTA TCAGGCCCCC
    GGCGGCCCTG ACCATCCCAA TCCCTCCAGT CCCTCCTCCT GTTCCTCCTC CTCCTCTTCC
    TCCTCTGCCC TGAAACGCCA CCTGTCTGCG CGCGCCAGTC GCCCCATGTC TATGTATGAA
    ACCGGCTCTG GCCTGAAGCC CTTTCTTCCC AAGGGCGAGA CCCCTTACCC TGAGGAAACC
    TTCCCCACCC TGCAACCCTT CCCAGCCTAT GCCTCCAAAT TTGACAAGCA GAGCAGCGTG
    TCTGATGGCG ACTACGACAA CACCGTCAAC GAATCTGATC TGGACGACTC TGGTTTTGGC
    CGGCGATGTC GCCTTCGCAG CAGTGGCTGG ATGGGAGAAA GCGGCTCTAT CCCCGAGCTG
    GAGGACCATG AGTGTGAGCT GGACCCCAGT CTGCCCAGCA CCGAAGACGT CATACGCAAA
    ACTGAGCAGA TCACCAAGAA CATTCAGGAG CTGCTGAGGG CCGCGCAGGA GAACAAGCAC
    GAGAGCTTTG TACCCTGCGC AGAAAGAATA CATGTGGCTG TGAATGAGAT GGCGGCTCTG
    TTCCCCAAGA GACCACGTTC TGAGACGGTC AGGAACTCTC TGCGGTTATT AACGTCCAGT
    GCGAATCGGC TCCAGACCGA GTGTAAGAAA GCGTCTCCTC TGGAGAGCAG CCAGGCCTCA
    GACATGCAGT TGGTCACTCA GCAGGTCATC CAGTGTGCTT ACGACATCGC CAAGGCTGCA
    AAGCAACTAG TTACTATAAC CACCAAAGAG AACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46849
    Clone ID Related Accession (Same CDS sequence) XM_009301357.2 , XM_009301357.3
    Accession Version XM_009301357.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ARF GTPase-activating protein GIT2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301357.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGCT GCAGTCTCTG
    CAAAGCGAGA ACAGCTCTCT GAGGCGGCAG GTCTCCGCTC AACAGCCCTA TCAGGCCCCC
    GGCGGCCCTG ACCATCCCAA TCCCTCCAGT CCCTCCTCCT GTTCCTCCTC CTCCTCTTCC
    TCCTCTGCCC TGAAACGCCA CCTGTCTGCG CGCGCCAGTC GCCCCATGTC TATGTATGAA
    ACCGGCTCTG GCCTGAAGCC CTTTCTTCCC AAGGGCGAGA CCCCTTACCC TGAGGAAACC
    TTCCCCACCC TGCAACCCTT CCCAGCCTAT GCCTCCAAAT TTGACAAGCA GAGCAGCGTG
    TCTGATGGCG ACTACGACAA CACCGTCAAC GAATCTGATC TGGACGACTC TGGTTTTGGC
    CGGCGATGTC GCCTTCGCAG CAGTGGCTGG ATGGGAGAAA GCGGCTCTAT CCCCGAGCTG
    GAGGACCATG AGTGTGAGCT GGACCCCAGT CTGCCCAGCA CCGAAGACGT CATACGCAAA
    ACTGAGCAGA TCACCAAGAA CATTCAGGAG CTGCTGAGGG CCGCGCAGGA GAACAAGCAC
    GAGAGCTTTG TACCCTGCGC AGAAAGAATA CATGTGGCTG TGAATGAGAT GGCGGCTCTG
    TTCCCCAAGA GACCACGTTC TGAGACGGTC AGGAACTCTC TGCGGTTATT AACGTCCAGT
    GCGAATCGGC TCCAGACCGA GTGTAAGAAA GCGTCTCCTC TGGAGAGCAG CCAGGCCTCA
    GACATGCAGT TGGTCACTCA GCAGGTCATC CAGTGTGCTT ACGACATCGC CAAGGCTGCA
    AAGCAACTAG TTACTATAAC CACCAAAGAG AACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299632.1
    CDS590..2848
    Translation

    Target ORF information:

    RefSeq Version XM_009301357.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301357.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGCT GCAGTCTCTG
    CAAAGCGAGA ACAGCTCTCT GAGGCGGCAG GTCTCCGCTC AACAGCCCTA TCAGGCCCCC
    GGCGGCCCTG ACCATCCCAA TCCCTCCAGT CCCTCCTCCT GTTCCTCCTC CTCCTCTTCC
    TCCTCTGCCC TGAAACGCCA CCTGTCTGCG CGCGCCAGTC GCCCCATGTC TATGTATGAA
    ACCGGCTCTG GCCTGAAGCC CTTTCTTCCC AAGGGCGAGA CCCCTTACCC TGAGGAAACC
    TTCCCCACCC TGCAACCCTT CCCAGCCTAT GCCTCCAAAT TTGACAAGCA GAGCAGCGTG
    TCTGATGGCG ACTACGACAA CACCGTCAAC GAATCTGATC TGGACGACTC TGGTTTTGGC
    CGGCGATGTC GCCTTCGCAG CAGTGGCTGG ATGGGAGAAA GCGGCTCTAT CCCCGAGCTG
    GAGGACCATG AGTGTGAGCT GGACCCCAGT CTGCCCAGCA CCGAAGACGT CATACGCAAA
    ACTGAGCAGA TCACCAAGAA CATTCAGGAG CTGCTGAGGG CCGCGCAGGA GAACAAGCAC
    GAGAGCTTTG TACCCTGCGC AGAAAGAATA CATGTGGCTG TGAATGAGAT GGCGGCTCTG
    TTCCCCAAGA GACCACGTTC TGAGACGGTC AGGAACTCTC TGCGGTTATT AACGTCCAGT
    GCGAATCGGC TCCAGACCGA GTGTAAGAAA GCGTCTCCTC TGGAGAGCAG CCAGGCCTCA
    GACATGCAGT TGGTCACTCA GCAGGTCATC CAGTGTGCTT ACGACATCGC CAAGGCTGCA
    AAGCAACTAG TTACTATAAC CACCAAAGAG AACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46851
    Clone ID Related Accession (Same CDS sequence) XM_009301359.3 , XM_009301359.2
    Accession Version XM_009301359.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ARF GTPase-activating protein GIT2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGGC CTCCAAATTT
    GACAAGCAGA GCAGCGTGTC TGATGGCGAC TACGACAACA CCGTCAACGA ATCTGATCTG
    GACGACTCTG GTTTTGGCCG GCGATGTCGC CTTCGCAGCA GTGGCTGGAT GGGAGAAAGC
    GGCTCTATCC CCGAGCTGGA GGACCATGAG TGTGAGCTGG ACCCCAGTCT GCCCAGCACC
    GAAGACGTCA TACGCAAAAC TGAGCAGATC ACCAAGAACA TTCAGGAGCT GCTGAGGGCC
    GCGCAGGAGA ACAAGCACGA GAGTGCACCA TGTGAGCAGA GGGAGAGTGT TCGCAGACTT
    AGACACAGTC TGGGTTGTTT CAGCACACTG GTGCCTTGGG CAGACAAACC CCCGTCTCCC
    ATGCAGCCCC TTGGCCTCAG AGCTCCCCAT CCAGCCAACC CCAACAACCC TGCCTCCTGC
    TTTGTACCCT GCGCAGAAAG AATACATGTG GCTGTGAATG AGATGGCGGC TCTGTTCCCC
    AAGAGACCAC GTTCTGAGAC GGTCAGGAAC TCTCTGCGGT TATTAACGTC CAGTGCGAAT
    CGGCTCCAGA CCGAGTGTAA GAAAGCGTCT CCTCTGGAGA GCAGCCAGGC CTCAGACATG
    CAGTTGGTCA CTCAGCAGGT CATCCAGTGT GCTTACGACA TCGCCAAGGC TGCAAAGCAA
    CTAGTTACTA TAACCACCAA AGAGAACAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299634.1
    CDS590..2722
    Translation

    Target ORF information:

    RefSeq Version XM_009301359.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301359.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGGC CTCCAAATTT
    GACAAGCAGA GCAGCGTGTC TGATGGCGAC TACGACAACA CCGTCAACGA ATCTGATCTG
    GACGACTCTG GTTTTGGCCG GCGATGTCGC CTTCGCAGCA GTGGCTGGAT GGGAGAAAGC
    GGCTCTATCC CCGAGCTGGA GGACCATGAG TGTGAGCTGG ACCCCAGTCT GCCCAGCACC
    GAAGACGTCA TACGCAAAAC TGAGCAGATC ACCAAGAACA TTCAGGAGCT GCTGAGGGCC
    GCGCAGGAGA ACAAGCACGA GAGTGCACCA TGTGAGCAGA GGGAGAGTGT TCGCAGACTT
    AGACACAGTC TGGGTTGTTT CAGCACACTG GTGCCTTGGG CAGACAAACC CCCGTCTCCC
    ATGCAGCCCC TTGGCCTCAG AGCTCCCCAT CCAGCCAACC CCAACAACCC TGCCTCCTGC
    TTTGTACCCT GCGCAGAAAG AATACATGTG GCTGTGAATG AGATGGCGGC TCTGTTCCCC
    AAGAGACCAC GTTCTGAGAC GGTCAGGAAC TCTCTGCGGT TATTAACGTC CAGTGCGAAT
    CGGCTCCAGA CCGAGTGTAA GAAAGCGTCT CCTCTGGAGA GCAGCCAGGC CTCAGACATG
    CAGTTGGTCA CTCAGCAGGT CATCCAGTGT GCTTACGACA TCGCCAAGGC TGCAAAGCAA
    CTAGTTACTA TAACCACCAA AGAGAACAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46851
    Clone ID Related Accession (Same CDS sequence) XM_009301359.3 , XM_009301359.2
    Accession Version XM_009301359.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ARF GTPase-activating protein GIT2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGGC CTCCAAATTT
    GACAAGCAGA GCAGCGTGTC TGATGGCGAC TACGACAACA CCGTCAACGA ATCTGATCTG
    GACGACTCTG GTTTTGGCCG GCGATGTCGC CTTCGCAGCA GTGGCTGGAT GGGAGAAAGC
    GGCTCTATCC CCGAGCTGGA GGACCATGAG TGTGAGCTGG ACCCCAGTCT GCCCAGCACC
    GAAGACGTCA TACGCAAAAC TGAGCAGATC ACCAAGAACA TTCAGGAGCT GCTGAGGGCC
    GCGCAGGAGA ACAAGCACGA GAGTGCACCA TGTGAGCAGA GGGAGAGTGT TCGCAGACTT
    AGACACAGTC TGGGTTGTTT CAGCACACTG GTGCCTTGGG CAGACAAACC CCCGTCTCCC
    ATGCAGCCCC TTGGCCTCAG AGCTCCCCAT CCAGCCAACC CCAACAACCC TGCCTCCTGC
    TTTGTACCCT GCGCAGAAAG AATACATGTG GCTGTGAATG AGATGGCGGC TCTGTTCCCC
    AAGAGACCAC GTTCTGAGAC GGTCAGGAAC TCTCTGCGGT TATTAACGTC CAGTGCGAAT
    CGGCTCCAGA CCGAGTGTAA GAAAGCGTCT CCTCTGGAGA GCAGCCAGGC CTCAGACATG
    CAGTTGGTCA CTCAGCAGGT CATCCAGTGT GCTTACGACA TCGCCAAGGC TGCAAAGCAA
    CTAGTTACTA TAACCACCAA AGAGAACAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299634.1
    CDS589..2721
    Translation

    Target ORF information:

    RefSeq Version XM_009301359.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301359.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGGC CTCCAAATTT
    GACAAGCAGA GCAGCGTGTC TGATGGCGAC TACGACAACA CCGTCAACGA ATCTGATCTG
    GACGACTCTG GTTTTGGCCG GCGATGTCGC CTTCGCAGCA GTGGCTGGAT GGGAGAAAGC
    GGCTCTATCC CCGAGCTGGA GGACCATGAG TGTGAGCTGG ACCCCAGTCT GCCCAGCACC
    GAAGACGTCA TACGCAAAAC TGAGCAGATC ACCAAGAACA TTCAGGAGCT GCTGAGGGCC
    GCGCAGGAGA ACAAGCACGA GAGTGCACCA TGTGAGCAGA GGGAGAGTGT TCGCAGACTT
    AGACACAGTC TGGGTTGTTT CAGCACACTG GTGCCTTGGG CAGACAAACC CCCGTCTCCC
    ATGCAGCCCC TTGGCCTCAG AGCTCCCCAT CCAGCCAACC CCAACAACCC TGCCTCCTGC
    TTTGTACCCT GCGCAGAAAG AATACATGTG GCTGTGAATG AGATGGCGGC TCTGTTCCCC
    AAGAGACCAC GTTCTGAGAC GGTCAGGAAC TCTCTGCGGT TATTAACGTC CAGTGCGAAT
    CGGCTCCAGA CCGAGTGTAA GAAAGCGTCT CCTCTGGAGA GCAGCCAGGC CTCAGACATG
    CAGTTGGTCA CTCAGCAGGT CATCCAGTGT GCTTACGACA TCGCCAAGGC TGCAAAGCAA
    CTAGTTACTA TAACCACCAA AGAGAACAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142700
    Clone ID Related Accession (Same CDS sequence) XM_021476320.1
    Accession Version XM_021476320.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ARF GTPase-activating protein GIT2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGCT GCAGTCTCTG
    CAAAGCGAGA ACAGCTCTCT GAGGCGGCAG GTCTCCGCTC AACAGCCCTA TCAGGCCCCC
    GGCGGCCCTG ACCATCCCAA TCCCTCCAGT CCCTCCTCCT GTTCCTCCTC CTCCTCTTCC
    TCCTCTGCCC TGAAACGCCA CCTGTCTGCG CGCGCCAGTC GCCCCATGTC TATGTATGAA
    ACCGGCTCTG GCCTGAAGCC CTTTCTTCCC AAGGGCGAGA CCCCTTACCC TGAGGAAACC
    TTCCCCACCC TGCAACCCTT CCCAGCCTAT GCCTCCAAAT TTGACAAGCA GAGCAGCGTG
    TCTGATGGCG ACTACGACAA CACCGTCAAC GAATCTGATC TGGACGACTC TGGTTTTGGC
    CGGCGATGTC GCCTTCGCAG CAGTGGCTGG ATGGGAGAAA GCGGCTCTAT CCCCGAGCTG
    GAGGACCATG AGTGTGAGCT GGACCCCAGT CTGCCCAGCA CCGAAGACGT CATACGCAAA
    ACTGAGCAGA TCACCAAGAA CATTCAGGAG CTGCTGAGGG CCGCGCAGGA GAACAAGCAC
    GAGAGTGCAC CATGTGAGCA GAGGGAGAGT GTTCGCAGAC TTAGACACAG TCTGGGTTGT
    TTCAGCACAC TGGTGCCTTG GGCAGACAAA CCCCCGTCTC CCATGCAGCC CCTTGGCCTC
    AGAGCTCCCC ATCCAGCCAA CCCCAACAAC CCTGCCTCCT GGTACTCTGG CCTCTGTCCC
    TGCCTCACAG GCACAGTGTC TACCTCTACC TCTCTCTTCA GAACTCACCC ACTAATCTCA
    ACACGAATGT CACCTGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331995.1
    CDS590..2830
    Translation

    Target ORF information:

    RefSeq Version XM_021476320.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio G protein-coupled receptor kinase interacting ArfGAP 2a (git2a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476320.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGTCATAC CCACGCTAAC GGAGACAAGG ATGTCGAAAC GGCTGCGAAA TAGTGAAGTT 
    TGTGCGGACT GCAGCACTTC AGATCCTCGC TGGGCGTCTG TGAACAGAGG AGTGTTGATA
    TGTGATGAGT GCTGTAGTGT TCACCGCAGT CTGGGTCGCC ACAATTCACA AGTGCGCCAT
    CTGTCAAACA CATCTTGGCC GCCCACCCAA CTACAGATGG TTCAGACATT ATACAACAAT
    GGCGCTAATG CAATATGGGA GCACACTCTG CTGGACCCGT CATCCATCAT GAGCAGCAAA
    CGCAAGCCTA ATCATCAGGA TAAACTCCAC CCCAACAAGG CAGACTTCAT CAAAGCAAAG
    TATCAAATGC TGGCTTACGT CCATCGTTTA CCATGTCGAG ATGATGACAG TACAGCTGCC
    GCTGATCTAA GCAAGCAACT TCACTCCAGC GTTCGAACAG GCAATCTTGA GACTTGTTTG
    CGGTTACTGT CTCTTGGGGC TCAAGCTAAC TACTTTCATC CGGAGAAAGG GAACACTCCG
    CTGCACATCG CGGCTAAAGC AGGTCAAGTG TGCCAGGCTG AGCTTCTTTG CGTTTATGGG
    GCAGATCCTG GAGCTCCAGA CTCCAGCGGC AAAACACCTA TCAACTATGC AAGGCAGGCT
    GGCCATCAGG ACCTGGCAGA CAGACTGGTG GAAATACAGT ACGAGCTGAC AGACAGGCTG
    GCCTTTTACC TCTGTGGGCG GAAGCCTGAT CACAAGAGTG GACAGCATTT CATTATACCA
    CAGATGGCAG ACAGAAATGT CAGTGTGGAG TTAACAGAGT TTGCAAAAGC TGCAAAAAGA
    AAACTACAGT CTCTCAGCGA TCACTTGTTT GAGGAATTGG CAATGGATGT TTATGATGAA
    GTAGACCGAC GAGAGACAGA TGCAGTTTGG CTGGCCACTC AGAACCACAG CACCTTGGTA
    ACAGAGACAA CAGTTGTGCC TTTCCTTCCT GTCAACCCTG AGTACTCATC AACACGAAAC
    CAGGGAAGAC AAAAGCTGGC AAGATTCAAC GCTCATGAAT TTGCCACACT TGTGATCGAT
    ATTCTAAGTG ATGCAAAGCG ACGCCAGCAT GGAAATCCAG TCCCAAGTCC TAAAGACAAT
    GTTGATGTAT TCTTCAAAAG TGGGGGCAGT CGTCATGGAA GTGAAAGTCA GGAAATTGAC
    CAGCCAGATT ATGACAGCGT GGCGTCCGAT GAGGACACCG ATACTGATTT ACGAATGGGC
    AAAGCAGACA GAACTAAAAG TCTGGACTCG GATCTCTCCG ATGGACCGGT TTCCGTGCAG
    GAGTTTCTGG AGGTGAAAAA CGCACTCTCA GCCTCGGAGG CCAAAATTCA GGAGCTGATG
    AAGGCCAACA GTAATCTGAA TGAAGAGCTG AGATTAATGC AGAAAAAGCT GCAGTCTCTG
    CAAAGCGAGA ACAGCTCTCT GAGGCGGCAG GTCTCCGCTC AACAGCCCTA TCAGGCCCCC
    GGCGGCCCTG ACCATCCCAA TCCCTCCAGT CCCTCCTCCT GTTCCTCCTC CTCCTCTTCC
    TCCTCTGCCC TGAAACGCCA CCTGTCTGCG CGCGCCAGTC GCCCCATGTC TATGTATGAA
    ACCGGCTCTG GCCTGAAGCC CTTTCTTCCC AAGGGCGAGA CCCCTTACCC TGAGGAAACC
    TTCCCCACCC TGCAACCCTT CCCAGCCTAT GCCTCCAAAT TTGACAAGCA GAGCAGCGTG
    TCTGATGGCG ACTACGACAA CACCGTCAAC GAATCTGATC TGGACGACTC TGGTTTTGGC
    CGGCGATGTC GCCTTCGCAG CAGTGGCTGG ATGGGAGAAA GCGGCTCTAT CCCCGAGCTG
    GAGGACCATG AGTGTGAGCT GGACCCCAGT CTGCCCAGCA CCGAAGACGT CATACGCAAA
    ACTGAGCAGA TCACCAAGAA CATTCAGGAG CTGCTGAGGG CCGCGCAGGA GAACAAGCAC
    GAGAGTGCAC CATGTGAGCA GAGGGAGAGT GTTCGCAGAC TTAGACACAG TCTGGGTTGT
    TTCAGCACAC TGGTGCCTTG GGCAGACAAA CCCCCGTCTC CCATGCAGCC CCTTGGCCTC
    AGAGCTCCCC ATCCAGCCAA CCCCAACAAC CCTGCCTCCT GGTACTCTGG CCTCTGTCCC
    TGCCTCACAG GCACAGTGTC TACCTCTACC TCTCTCTTCA GAACTCACCC ACTAATCTCA
    ACACGAATGT CACCTGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.