nkd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nkd1
Entrez Gene ID	751108
Full Name	naked cuticle homolog 1 (Drosophila)
General protein information	Preferred Names naked cuticle homolog 1 (Drosophila) Names protein naked cuticle homolog 1 naked 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005169004.3	XP_005169061.1	protein naked cuticle homolog 1 isoform X1
NM_001043333.1	NP_001036798.1	protein naked cuticle homolog 1
XM_005169004.4	XP_005169061.1	protein naked cuticle homolog 1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NKD1	NP_001098923.1
Mus musculus (house mouse)	Nkd1	NP_081556.3
Rattus norvegicus (Norway rat)	Nkd1	NP_001258310.1
Xenopus tropicalis (tropical clawed frog)	nkd1	NP_001016798.1
Canis lupus familiaris (dog)	NKD1	XP_544414.3
Danio rerio (zebrafish)	nkd1	NP_001036798.1
Homo sapiens (human)	NKD1	NP_149110.1
Pan troglodytes (chimpanzee)	NKD1	XP_520634.2
Gallus gallus (chicken)	NKD1	XP_414094.3
	NKD1	XP_001086556.2

Gene Ontology
Bibliography

Related articles in PubMed

The Nkd EF-hand domain modulates divergent wnt signaling outputs in zebrafish.
Marsden AN, Derry SW, Schneider I, Scott CA, Westfall TA, Brastrom LK, Shea MA, Dawson DV, Slusarski DC
Developmental biology(2017 Nov)

Copper impairs zebrafish swimbladder development by down-regulating Wnt signaling.
Xu J, Zhang R, Zhang T, Zhao G, Huang Y, Wang H, Liu JX
Aquatic toxicology (Amsterdam, Netherlands)192155-164(2017 Nov)

Rnf25/AO7 positively regulates wnt signaling via disrupting Nkd1-Axin inhibitory complex independent of its ubiquitin ligase activity.
Gao R, Ma LQ, Du X, Zhang TT, Zhao L, Liu L, Liu JC, Guo F, Cheng Z, Huang H
Oncotarget7(17)23850-9(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Wnt ligand-dependent activation of the negative feedback regulator Nkd1.
Larraguibel J, Weiss AR, Pasula DJ, Dhaliwal RS, Kondra R, Van Raay TJ
Molecular biology of the cell26(12)2375-84(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nkd1 activity is specifically dependent on Wnt ligand activation of the receptor. Nkd1 is recruited to the Wnt signalosome with Dvl2, where it becomes activated to move into the cytoplasm to interact with beta-catenin, inhibiting its nuclear accumulation.
Title: Wnt ligand-dependent activation of the negative feedback regulator Nkd1.

Nkd1 functions as a passive antagonist of Wnt signaling, functioning only when homeostatic levels of Wnt signaling have been breached or when Wnt signaling becomes destabilized.
Title: Nkd1 functions as a passive antagonist of Wnt signaling.

Data demonstrate that Nkd1 binds to beta-catenin and prevents its nuclear accumulation. Nkd1 function is dependent on its interaction with the cell membrane.
Title: Naked1 antagonizes Wnt signaling by preventing nuclear accumulation of ?-catenin.

Nkd1 knockdown in the dorsal forerunner cells causes beta-catenin nuclear localization/transcriptional activation & changes in DFC migration, Kupffer's vesicle formation, ciliogenesis, left-right patterning, & asymmetric charon expression.
Title: Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning.

data indicate that zebrafish Nkd1 and Nkd2 function to limit both canonical and non-canonical Wnt signaling
Title: Zebrafish Naked1 and Naked2 antagonize both canonical and non-canonical Wnt signaling.

The following nkd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nkd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01196	NM_001043333.1 Latest version!	Danio rerio NKD inhibitor of WNT signaling pathway 1 (nkd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa13233	XM_005169004.4 Latest version!	Danio rerio naked cuticle homolog 1 (Drosophila) (nkd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa13233	XM_005169004.3	Danio rerio naked cuticle homolog 1 (Drosophila) (nkd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nkd1 ( NM_001043333.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001036798.1 Danio rerio NKD inhibitor of WNT signaling pathway 1 (nkd1), mRNA.

Price & Availability↑

CloneID	ODa01196
Clone ID Related Accession (Same CDS sequence)	NM_001043333.1
Accession Version	NM_001043333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	protein naked cuticle homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY863053.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY863053.1, BC163386.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGTAAAC TTCATTCCAA ACATGCTGCT ATCTGTAAAG CGAGGGAGAG TCCTGAAGGC 
GATAGTTTTG TGGTTAACGC GTGTTTGGCG AGGAAAGGAC TGGATGACTG GATGGTTAAA 
CAGAAGTATT ACTGCTCGAG CTCTCGTGTT GATCAGCGGG ACTGCCAGCA GAAAAACAAC 
TGCGAGCTGT CTCCACGGGA TCCTCTGGAT GAAGGATATG CTGAAGGTAT CAGCGATGAA 
CATTATCGTT TAGAAGTGGC TCTGCCCCCT GAGAAGACAG ACAGCTGCAG TGTGGAAGAG 
AGAATGCAGG GCACTGAAGG CTCTGCCACT GTCGGCCCAC ACAAACAACT ACAGTTTGAG 
GAGCTGGAGT GTGCTGTATC AGTGGAAGAA GATAACAGAC AAGAGTGGAC TTTCACTCTC 
TACGACTTTG ATAACAATGG CAAGGTCACA AGAGAGGACA TCACAAGTCT TCTGCACACT 
ATATATGAAG TGGTGGACGC GTCAGTGAAT CACTCTCCCA GCAGCAGTAA GACCCTCAGA 
GTCAAACTGT CTGTGGCTCC AGACTCCAGC CAGAGATGGA AGAACTGCAC TCAGACTAAC 
ACAGACACCC CCAATCCCAA GCATAAGGGA GAAAAGTGCA TAGAGGACTC CAAGACTTCA 
GAGAAGAAGT CAAGAGCTTT TCTTAGACGT TATCATGCCG AGCACCACAG CCAGCAGGGT 
TGCCAGCGGC ACTGTGTGGA TGAGAACCTG GAGAGAAGAA ACCATTATCT TGACCTGGCC 
GGCATTGAAA ACTACACATC CCGCTTTGGA ACAGCAGCTC CTGCCACCGA ACCGGCAAAA 
CCTACCCACG CCACCCGTTC GTCCAATCAG AGCCGCTCGC GCTCTCAAGA ACCAGAGAAT 
GGCCAGGTTC ACCCGGCTCA CCATCGCCGC TCACAGACCA TGTCCACAGA TCCTCCTGCC 
ATCCTTACCC GACACACACA TGCTCTTCGA TCCCCGAAAA CCCACCGCAC GCCACCCACC 
CAGGCCTCAG CAGGCCGAGT TATGAGGAGA GGAGCACCAC CTCCCCCATC GGTCCCCAAC 
CAGACCCCTC CACACCAAAG CTCAGGCCCA TATCGACGAC ACAAGCAGAG GCCTAAAGAA 
GCGCTGCACT ACAGGGCCCT GGGCCCGAGT GTGACATCCG GCCCAGTGCT GGAGAAAGAA 
CACGTGAGGG ATCTGCCCAG TCTGGTGCTT TATGAAGGAG GACTCGCTCA AGTTGTCCAG 
AGACACGAGC ATCATCATCA TCACGAGCAT CACCATCACT ATCATCACTT CTACCAGTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036798.1
CDS	140..1462
Translation	MGKLHSKHAAICKARESPEGDSFVVNACLARKGLDDWMVKQKYYCSSSRVDQRDCQQKNNCELSPRDPLDEGYAEGISDEHYRLEVALPPEKTDSCSVEERMQGTEGSATVGPHKQLQFEELECAVSVEEDNRQEWTFTLYDFDNNGKVTREDITSLLHTIYEVVDASVNHSPSSSKTLRVKLSVAPDSSQRWKNCTQTNTDTPNPKHKGEKCIEDSKTSEKKSRAFLRRYHAEHHSQQGCQRHCVDENLERRNHYLDLAGIENYTSRFGTAAPATEPAKPTHATRSSNQSRSRSQEPENGQVHPAHHRRSQTMSTDPPAILTRHTHALRSPKTHRTPPTQASAGRVMRRGAPPPPSVPNQTPPHQSSGPYRRHKQRPKEALHYRALGPSVTSGPVLEKEHVRDLPSLVLYEGGLAQVVQRHEHHHHHEHHHHYHHFYQS

Target ORF information:

RefSeq Version	NM_001043333.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NKD inhibitor of WNT signaling pathway 1 (nkd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043333.1

ORF Insert Sequence:

ATGGGTAAAC TTCATTCCAA ACATGCTGCT ATCTGTAAAG CGAGGGAGAG TCCTGAAGGC 
GATAGTTTTG TGGTTAACGC GTGTTTGGCG AGGAAAGGAC TGGATGACTG GATGGTTAAA 
CAGAAGTATT ACTGCTCGAG CTCTCGTGTT GATCAGCGGG ACTGCCAGCA GAAAAACAAC 
TGCGAGCTGT CTCCACGGGA TCCTCTGGAT GAAGGATATG CTGAAGGTAT CAGCGATGAA 
CATTATCGTT TAGAAGTGGC TCTGCCCCCT GAGAAGACAG ACAGCTGCAG TGTGGAAGAG 
AGAATGCAGG GCACTGAAGG CTCTGCCACT GTCGGCCCAC ACAAACAACT ACAGTTTGAG 
GAGCTGGAGT GTGCTGTATC AGTGGAAGAA GATAACAGAC AAGAGTGGAC TTTCACTCTC 
TACGACTTTG ATAACAATGG CAAGGTCACA AGAGAGGACA TCACAAGTCT TCTGCACACT 
ATATATGAAG TGGTGGACGC GTCAGTGAAT CACTCTCCCA GCAGCAGTAA GACCCTCAGA 
GTCAAACTGT CTGTGGCTCC AGACTCCAGC CAGAGATGGA AGAACTGCAC TCAGACTAAC 
ACAGACACCC CCAATCCCAA GCATAAGGGA GAAAAGTGCA TAGAGGACTC CAAGACTTCA 
GAGAAGAAGT CAAGAGCTTT TCTTAGACGT TATCATGCCG AGCACCACAG CCAGCAGGGT 
TGCCAGCGGC ACTGTGTGGA TGAGAACCTG GAGAGAAGAA ACCATTATCT TGACCTGGCC 
GGCATTGAAA ACTACACATC CCGCTTTGGA ACAGCAGCTC CTGCCACCGA ACCGGCAAAA 
CCTACCCACG CCACCCGTTC GTCCAATCAG AGCCGCTCGC GCTCTCAAGA ACCAGAGAAT 
GGCCAGGTTC ACCCGGCTCA CCATCGCCGC TCACAGACCA TGTCCACAGA TCCTCCTGCC 
ATCCTTACCC GACACACACA TGCTCTTCGA TCCCCGAAAA CCCACCGCAC GCCACCCACC 
CAGGCCTCAG CAGGCCGAGT TATGAGGAGA GGAGCACCAC CTCCCCCATC GGTCCCCAAC 
CAGACCCCTC CACACCAAAG CTCAGGCCCA TATCGACGAC ACAAGCAGAG GCCTAAAGAA 
GCGCTGCACT ACAGGGCCCT GGGCCCGAGT GTGACATCCG GCCCAGTGCT GGAGAAAGAA 
CACGTGAGGG ATCTGCCCAG TCTGGTGCTT TATGAAGGAG GACTCGCTCA AGTTGTCCAG 
AGACACGAGC ATCATCATCA TCACGAGCAT CACCATCACT ATCATCACTT CTACCAGTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkd1 ( XM_005169004.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169061.1 Danio rerio naked cuticle homolog 1 (Drosophila) (nkd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa13233
Clone ID Related Accession (Same CDS sequence)	XM_005169004.3 , XM_005169004.4
Accession Version	XM_005169004.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein naked cuticle homolog 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169004.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAAC TTCATTCCAA ACATGCTGCT ATCTGTAAAG CGAGGGAGAG TCCTGAAGGC 
GATAGTTTTG TAGTTAACGC GTGTTTGGCG AGGAAAGGAC TGGATGACTG GATGGTTAAA 
CAGAAGTATT ACTGCTCGAG CTCTCGTGTT GATCAGCGGG ACTGCCAGCA GAAAAACAAC 
TGCGAGCTGT CTCCACGGGA TCCTCTGGAT GAAGGATATG CTGAAGGTAT CAGCGATGAA 
CATTATCGTT TAGAAGTGGC TCTGCCCCCT GAGAAGACAG ACAGCTGCAG TGTGGAAGAG 
AGAATGCAGG GCACTGAAGG CTCTGCCACT GTCGGCCCAC ACAAACAACT ACAGTTTGAG 
GAGCTGGAGT GTGCTGTATC AGTGGAAGAA GATAACAGAC AAGAGTGGAC TTTCACTCTC 
TACGACTTTG ATAACAATGG CAAGGTCACA AGAGAGGACA TCACAAGTCT TCTGCACACT 
ATATATGAAG TGGTGGACGC GTCAGTGAAT CACTCTCCCA GCAGCAGTAA GACCCTCAGA 
GTCAAACTGT CTGTGGCTCC AGACTCCAGC CAGAGATGGA AGAACTGCAC TCAGACTAAC 
ACAGACACCC CCAATCCCAA GCATAAGGGA GAAAAGTGCA TAGAGGACTC CAAGACTTCA 
GAGAAGAAGT CAAGAGCTTT TCTTAGACGT TATCATGCCG AGCACCACAG CCAGCAGGGT 
TGCCAGCGGC ACTGTGTGGA TGAGAACCTG GAGAGAAGAA ACCATTATCT TGACCTGGCC 
GGCATTGAAA ACTACACATC CCGCTTTGGA ACAGCTCCTG CCACCGAACC GGCAAAACCT 
ACCCACGCCA CCCGTTCGTC CAATCAGAGC CGCTCGCGCT CTCAAGAACC AGAGAATGGC 
CAGGTTCACC CGGCTCACCA TCGCCGCTCA CAGACCATGT CCACAGATCC TCCTGCCATC 
CTTACCCGAC ACACACATGC TCTTCGATCC CCGAAAACCC ACCGCACGCC ACCCACCCAG 
GCCTCAGCAG GCCGAGTTAT GAGGAGAGGA GCACCACCTC CCCCATCGGT CCCCAACCAG 
ACCCCTCCAC ACCAAAGCTC AGGCCCATAT CGACGACACA AGCAGAGGCC TAAAGAAGCG 
CTGCACTACA GGGCCCTGGG CCCGAGTGTG ACATCCGGCC CAGTGCTGGA GAAAGAACAC 
GTGAGGGATC TGCCCAGTCT GGTGCTTTAT GAAGGAGGAC TCGCTCAAGT TGTCCAGAGA 
CACGAGCATC ATCATCATCA CGAGCATCAC CATCACTATC ATCACTTCTA CCAGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169061.1
CDS	159..1478
Translation	MGKLHSKHAAICKARESPEGDSFVVNACLARKGLDDWMVKQKYYCSSSRVDQRDCQQKNNCELSPRDPLDEGYAEGISDEHYRLEVALPPEKTDSCSVEERMQGTEGSATVGPHKQLQFEELECAVSVEEDNRQEWTFTLYDFDNNGKVTREDITSLLHTIYEVVDASVNHSPSSSKTLRVKLSVAPDSSQRWKNCTQTNTDTPNPKHKGEKCIEDSKTSEKKSRAFLRRYHAEHHSQQGCQRHCVDENLERRNHYLDLAGIENYTSRFGTAPATEPAKPTHATRSSNQSRSRSQEPENGQVHPAHHRRSQTMSTDPPAILTRHTHALRSPKTHRTPPTQASAGRVMRRGAPPPPSVPNQTPPHQSSGPYRRHKQRPKEALHYRALGPSVTSGPVLEKEHVRDLPSLVLYEGGLAQVVQRHEHHHHHEHHHHYHHFYQS

Target ORF information:

RefSeq Version	XM_005169004.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio naked cuticle homolog 1 (Drosophila) (nkd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169004.3

ORF Insert Sequence:

ATGGGTAAAC TTCATTCCAA ACATGCTGCT ATCTGTAAAG CGAGGGAGAG TCCTGAAGGC 
GATAGTTTTG TAGTTAACGC GTGTTTGGCG AGGAAAGGAC TGGATGACTG GATGGTTAAA 
CAGAAGTATT ACTGCTCGAG CTCTCGTGTT GATCAGCGGG ACTGCCAGCA GAAAAACAAC 
TGCGAGCTGT CTCCACGGGA TCCTCTGGAT GAAGGATATG CTGAAGGTAT CAGCGATGAA 
CATTATCGTT TAGAAGTGGC TCTGCCCCCT GAGAAGACAG ACAGCTGCAG TGTGGAAGAG 
AGAATGCAGG GCACTGAAGG CTCTGCCACT GTCGGCCCAC ACAAACAACT ACAGTTTGAG 
GAGCTGGAGT GTGCTGTATC AGTGGAAGAA GATAACAGAC AAGAGTGGAC TTTCACTCTC 
TACGACTTTG ATAACAATGG CAAGGTCACA AGAGAGGACA TCACAAGTCT TCTGCACACT 
ATATATGAAG TGGTGGACGC GTCAGTGAAT CACTCTCCCA GCAGCAGTAA GACCCTCAGA 
GTCAAACTGT CTGTGGCTCC AGACTCCAGC CAGAGATGGA AGAACTGCAC TCAGACTAAC 
ACAGACACCC CCAATCCCAA GCATAAGGGA GAAAAGTGCA TAGAGGACTC CAAGACTTCA 
GAGAAGAAGT CAAGAGCTTT TCTTAGACGT TATCATGCCG AGCACCACAG CCAGCAGGGT 
TGCCAGCGGC ACTGTGTGGA TGAGAACCTG GAGAGAAGAA ACCATTATCT TGACCTGGCC 
GGCATTGAAA ACTACACATC CCGCTTTGGA ACAGCTCCTG CCACCGAACC GGCAAAACCT 
ACCCACGCCA CCCGTTCGTC CAATCAGAGC CGCTCGCGCT CTCAAGAACC AGAGAATGGC 
CAGGTTCACC CGGCTCACCA TCGCCGCTCA CAGACCATGT CCACAGATCC TCCTGCCATC 
CTTACCCGAC ACACACATGC TCTTCGATCC CCGAAAACCC ACCGCACGCC ACCCACCCAG 
GCCTCAGCAG GCCGAGTTAT GAGGAGAGGA GCACCACCTC CCCCATCGGT CCCCAACCAG 
ACCCCTCCAC ACCAAAGCTC AGGCCCATAT CGACGACACA AGCAGAGGCC TAAAGAAGCG 
CTGCACTACA GGGCCCTGGG CCCGAGTGTG ACATCCGGCC CAGTGCTGGA GAAAGAACAC 
GTGAGGGATC TGCCCAGTCT GGTGCTTTAT GAAGGAGGAC TCGCTCAAGT TGTCCAGAGA 
CACGAGCATC ATCATCATCA CGAGCATCAC CATCACTATC ATCACTTCTA CCAGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nkd1 ( XM_005169004.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169061.1 Danio rerio naked cuticle homolog 1 (Drosophila) (nkd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa13233
Clone ID Related Accession (Same CDS sequence)	XM_005169004.3 , XM_005169004.4
Accession Version	XM_005169004.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein naked cuticle homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169004.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAAC TTCATTCCAA ACATGCTGCT ATCTGTAAAG CGAGGGAGAG TCCTGAAGGC 
GATAGTTTTG TAGTTAACGC GTGTTTGGCG AGGAAAGGAC TGGATGACTG GATGGTTAAA 
CAGAAGTATT ACTGCTCGAG CTCTCGTGTT GATCAGCGGG ACTGCCAGCA GAAAAACAAC 
TGCGAGCTGT CTCCACGGGA TCCTCTGGAT GAAGGATATG CTGAAGGTAT CAGCGATGAA 
CATTATCGTT TAGAAGTGGC TCTGCCCCCT GAGAAGACAG ACAGCTGCAG TGTGGAAGAG 
AGAATGCAGG GCACTGAAGG CTCTGCCACT GTCGGCCCAC ACAAACAACT ACAGTTTGAG 
GAGCTGGAGT GTGCTGTATC AGTGGAAGAA GATAACAGAC AAGAGTGGAC TTTCACTCTC 
TACGACTTTG ATAACAATGG CAAGGTCACA AGAGAGGACA TCACAAGTCT TCTGCACACT 
ATATATGAAG TGGTGGACGC GTCAGTGAAT CACTCTCCCA GCAGCAGTAA GACCCTCAGA 
GTCAAACTGT CTGTGGCTCC AGACTCCAGC CAGAGATGGA AGAACTGCAC TCAGACTAAC 
ACAGACACCC CCAATCCCAA GCATAAGGGA GAAAAGTGCA TAGAGGACTC CAAGACTTCA 
GAGAAGAAGT CAAGAGCTTT TCTTAGACGT TATCATGCCG AGCACCACAG CCAGCAGGGT 
TGCCAGCGGC ACTGTGTGGA TGAGAACCTG GAGAGAAGAA ACCATTATCT TGACCTGGCC 
GGCATTGAAA ACTACACATC CCGCTTTGGA ACAGCTCCTG CCACCGAACC GGCAAAACCT 
ACCCACGCCA CCCGTTCGTC CAATCAGAGC CGCTCGCGCT CTCAAGAACC AGAGAATGGC 
CAGGTTCACC CGGCTCACCA TCGCCGCTCA CAGACCATGT CCACAGATCC TCCTGCCATC 
CTTACCCGAC ACACACATGC TCTTCGATCC CCGAAAACCC ACCGCACGCC ACCCACCCAG 
GCCTCAGCAG GCCGAGTTAT GAGGAGAGGA GCACCACCTC CCCCATCGGT CCCCAACCAG 
ACCCCTCCAC ACCAAAGCTC AGGCCCATAT CGACGACACA AGCAGAGGCC TAAAGAAGCG 
CTGCACTACA GGGCCCTGGG CCCGAGTGTG ACATCCGGCC CAGTGCTGGA GAAAGAACAC 
GTGAGGGATC TGCCCAGTCT GGTGCTTTAT GAAGGAGGAC TCGCTCAAGT TGTCCAGAGA 
CACGAGCATC ATCATCATCA CGAGCATCAC CATCACTATC ATCACTTCTA CCAGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169061.1
CDS	158..1477
Translation	MGKLHSKHAAICKARESPEGDSFVVNACLARKGLDDWMVKQKYYCSSSRVDQRDCQQKNNCELSPRDPLDEGYAEGISDEHYRLEVALPPEKTDSCSVEERMQGTEGSATVGPHKQLQFEELECAVSVEEDNRQEWTFTLYDFDNNGKVTREDITSLLHTIYEVVDASVNHSPSSSKTLRVKLSVAPDSSQRWKNCTQTNTDTPNPKHKGEKCIEDSKTSEKKSRAFLRRYHAEHHSQQGCQRHCVDENLERRNHYLDLAGIENYTSRFGTAPATEPAKPTHATRSSNQSRSRSQEPENGQVHPAHHRRSQTMSTDPPAILTRHTHALRSPKTHRTPPTQASAGRVMRRGAPPPPSVPNQTPPHQSSGPYRRHKQRPKEALHYRALGPSVTSGPVLEKEHVRDLPSLVLYEGGLAQVVQRHEHHHHHEHHHHYHHFYQS

Target ORF information:

RefSeq Version	XM_005169004.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio naked cuticle homolog 1 (Drosophila) (nkd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169004.4

ORF Insert Sequence:

ATGGGTAAAC TTCATTCCAA ACATGCTGCT ATCTGTAAAG CGAGGGAGAG TCCTGAAGGC 
GATAGTTTTG TAGTTAACGC GTGTTTGGCG AGGAAAGGAC TGGATGACTG GATGGTTAAA 
CAGAAGTATT ACTGCTCGAG CTCTCGTGTT GATCAGCGGG ACTGCCAGCA GAAAAACAAC 
TGCGAGCTGT CTCCACGGGA TCCTCTGGAT GAAGGATATG CTGAAGGTAT CAGCGATGAA 
CATTATCGTT TAGAAGTGGC TCTGCCCCCT GAGAAGACAG ACAGCTGCAG TGTGGAAGAG 
AGAATGCAGG GCACTGAAGG CTCTGCCACT GTCGGCCCAC ACAAACAACT ACAGTTTGAG 
GAGCTGGAGT GTGCTGTATC AGTGGAAGAA GATAACAGAC AAGAGTGGAC TTTCACTCTC 
TACGACTTTG ATAACAATGG CAAGGTCACA AGAGAGGACA TCACAAGTCT TCTGCACACT 
ATATATGAAG TGGTGGACGC GTCAGTGAAT CACTCTCCCA GCAGCAGTAA GACCCTCAGA 
GTCAAACTGT CTGTGGCTCC AGACTCCAGC CAGAGATGGA AGAACTGCAC TCAGACTAAC 
ACAGACACCC CCAATCCCAA GCATAAGGGA GAAAAGTGCA TAGAGGACTC CAAGACTTCA 
GAGAAGAAGT CAAGAGCTTT TCTTAGACGT TATCATGCCG AGCACCACAG CCAGCAGGGT 
TGCCAGCGGC ACTGTGTGGA TGAGAACCTG GAGAGAAGAA ACCATTATCT TGACCTGGCC 
GGCATTGAAA ACTACACATC CCGCTTTGGA ACAGCTCCTG CCACCGAACC GGCAAAACCT 
ACCCACGCCA CCCGTTCGTC CAATCAGAGC CGCTCGCGCT CTCAAGAACC AGAGAATGGC 
CAGGTTCACC CGGCTCACCA TCGCCGCTCA CAGACCATGT CCACAGATCC TCCTGCCATC 
CTTACCCGAC ACACACATGC TCTTCGATCC CCGAAAACCC ACCGCACGCC ACCCACCCAG 
GCCTCAGCAG GCCGAGTTAT GAGGAGAGGA GCACCACCTC CCCCATCGGT CCCCAACCAG 
ACCCCTCCAC ACCAAAGCTC AGGCCCATAT CGACGACACA AGCAGAGGCC TAAAGAAGCG 
CTGCACTACA GGGCCCTGGG CCCGAGTGTG ACATCCGGCC CAGTGCTGGA GAAAGAACAC 
GTGAGGGATC TGCCCAGTCT GGTGCTTTAT GAAGGAGGAC TCGCTCAAGT TGTCCAGAGA 
CACGAGCATC ATCATCATCA CGAGCATCAC CATCACTATC ATCACTTCTA CCAGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Nkd EF-hand domain modulates divergent wnt signaling outputs in zebrafish.
Developmental biology(2017 Nov)
Marsden AN,Derry SW,Schneider I,Scott CA,Westfall TA,Brastrom LK,Shea MA,Dawson DV,Slusarski DC

Copper impairs zebrafish swimbladder development by down-regulating Wnt signaling.
Aquatic toxicology (Amsterdam, Netherlands)192155-164(2017 Nov)
Xu J,Zhang R,Zhang T,Zhao G,Huang Y,Wang H,Liu JX

Rnf25/AO7 positively regulates wnt signaling via disrupting Nkd1-Axin inhibitory complex independent of its ubiquitin ligase activity.
Oncotarget7(17)23850-9(2016 Apr)
Gao R,Ma LQ,Du X,Zhang TT,Zhao L,Liu L,Liu JC,Guo F,Cheng Z,Huang H

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Wnt ligand-dependent activation of the negative feedback regulator Nkd1.
Molecular biology of the cell26(12)2375-84(2015 Jun)
Larraguibel J,Weiss AR,Pasula DJ,Dhaliwal RS,Kondra R,Van Raay TJ

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nkd1 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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