runx3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	runx3
Entrez Gene ID	58127
Full Name	runt-related transcription factor 3
Synonyms	runxb
General protein information	Preferred Names runt-related transcription factor 3 Names runt-related transcription factor 3 runt-related transcription factor b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001362344.1	NP_001349273.1	runt-related transcription factor 3 isoform 1
NM_131604.3	NP_571679.2	runt-related transcription factor 3 isoform 2
NM_131604.2	NP_571679.2	runt-related transcription factor 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RUNX3	XP_003307937.1
Homo sapiens (human)	RUNX3	NP_001026850.1
Bos taurus (cattle)	RUNX3	NP_001180087.1
Mus musculus (house mouse)	Runx3	NP_062706.2
Xenopus tropicalis (tropical clawed frog)	runx3	XP_002936643.1
Canis lupus familiaris (dog)	LOC487366	XP_852730.2
Rattus norvegicus (Norway rat)	Runx3	NP_569109.1
Danio rerio (zebrafish)	runx3	NP_571679.2
Gallus gallus (chicken)	RUNX3	XP_001232978.3

Gene Ontology
Bibliography

Related articles in PubMed

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
Tang Q, Iyer S, Lobbardi R, Moore JC, Chen H, Lareau C, Hebert C, Shaw ML, Neftel C, Suva ML, Ceol CJ, Bernards A, Aryee M, Pinello L, Drummond IA, Langenau DM
The Journal of experimental medicine214(10)2875-2887(2017 Oct)

An ancient neurotrophin receptor code; a single Runx/Cbf? complex determines somatosensory neuron fate specification in zebrafish.
Gau P, Curtright A, Condon L, Raible DW, Dhaka A
PLoS genetics13(7)e1006884(2017 Jul)

Evolutionary and Expression Analyses Show Co-option of
Wang S, Yang Q, Wang Z, Feng S, Li H, Ji D, Zhang S
Frontiers in genetics8225(2017)

CD4-Transgenic Zebrafish Reveal Tissue-Resident Th2- and Regulatory T Cell-like Populations and Diverse Mononuclear Phagocytes.
Dee CT, Nagaraju RT, Athanasiadis EI, Gray C, Fernandez Del Ama L, Johnston SA, Secombes CJ, Cvejic A, Hurlstone AF
Journal of immunology (Baltimore, Md. : 1950)197(9)3520-3530(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In neuronal fate determination, Runx co-factor Cbfbeta is essential for its function, but the high level of Runx3 expression can overcome the loss of Cbfbeta, demonstrating that Cbfbeta in this context serves solely as a signal amplifier of Runx3 activity.
Title: An ancient neurotrophin receptor code; a single Runx/Cbf? complex determines somatosensory neuron fate specification in zebrafish.

successive induction of the transcription factors Runx3, Egr1 and Sox9b constitutes a regulatory cascade that controls expression of Follistatin A in pharyngeal endoderm, the latter modulating BMP signaling in developing cranial cartilage in zebrafish
Title: RUNX3, EGR1 and SOX9B form a regulatory cascade required to modulate BMP-signaling during cranial cartilage development in zebrafish.

Zebrafish embryos lacking Rad21, or cohesin subunit Smc3, fail to express runx3 and lose hematopoietic runx1 expression in early embryonic development.
Title: Cohesin-dependent regulation of Runx genes.

Runx3 expression leads to an increase in primitive blood cell numbers, together with an increase in runx1-expressing cells in the ventral wall of the dorsal aorta.
Title: Runx3 is required for hematopoietic development in zebrafish.

isolation and characterization by spatiotemporal expression detected in the developing zebrafish
Title: Isolation and characterization of runxa and runxb, zebrafish members of the runt family of transcriptional regulators.

The following runx3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the runx3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa05287	NM_131604.2 Latest version!	Danio rerio runt-related transcription factor 3 (runx3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa159840	NM_131604.3 Latest version!	Danio rerio RUNX family transcription factor 3 (runx3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa159874	NM_001362344.1 Latest version!	Danio rerio RUNX family transcription factor 3 (runx3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

runx3 ( NM_131604.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571679.2 Danio rerio runt-related transcription factor 3 (runx3), mRNA.

Price & Availability↑

CloneID	ODa05287
Clone ID Related Accession (Same CDS sequence)	NM_131604.2
Accession Version	NM_131604.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-02
Organism	Danio rerio(zebrafish)
Product	runt-related transcription factor 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF104037.1. On Dec 20, 2003 this sequence version replaced NM_131604.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF104037.1 [ECO:0000332] ##Evidence-Data-END##

ATGCATATTC CCGTAGACCC GAGCACGAGC AGACGGTTCA CGCCGCCCTC CGCCTCGTTC 
CCCTGCGCTA AAGTGACCGA GAGCCAGAGC ATGAGCGCGC CGGGCCCGCT GCGCTCCGGC 
CGGCCCGTGG AGAGCCGCTC CGTGGTGGAC GTGCTGGCTG ATCACGCCGG AGAGCTGGTG 
CGCACGGACA GCCCAAACTT TCTCTGCTCG GTCCTGCCGT CTCACTGGAG GTGCAACAAA 
ACCCTTCCCG TGGCGTTTAA GGTGGTGGCT CTTGGTGATG TTCCTGACGG GACTCTGGTC 
ACTGTGATGG CGGGAAATGA TGAGAATTAT TCCGCGGAGC TGAGAAACGC GTCTGCCGTC 
ATGAAGAACC AGGTGGCGCG CTTCAACGAC CTGCGCTTCG TGGGCAGGAG CGGCAGAGGA 
AAGAGCTTCA CTTTGACCAT CACTGTGTTC ACTGGACCGC CGCAAGTCGC CACGTACCAC 
CGCGCTATCA AAGTCACCGT TGACGGACCT CGAGAGCCAA GACGTCACCG CGTGAAGCCG 
GATGACCCTC ATAAGCAATT CTCCGACCGT CTGAGTGACA TCGAGCGCTT CCAGAGAGCC 
AGCCTGAGGA TGAACCCTGG AAACGGTACC ACCCGGCCCC ACCAAACCCA TTACAGCCCC 
TCCAGCACCA CACAGATCCC AGGATTATGG CCAGATCAGA TTGATCCTCC AACACTGAAG 
ACGTGCAAAG TGGAAGACGA CTTGCGCTGG ACAAGTACAG ATCTGTTCCA GCAGAGGACG 
TCTTTCCCGT CTCTGTCTCC ACTAACAGCG CCTCGTTTCT CTGACTCGCA CATGCATTAT 
CCGAGCCACT TCACATACTC CGCCAACCCG TCCAGCACTG GCATCGGGGG ACTGAGTGTG 
GCCGGTATGC CCACCTCTAG CCGCTACCAC ACCTACCTGC CCCCTCCCTA CCCGGGCAAC 
CAAAACCAGA ACTCCCACTT CCAGACCAAC TCCTCGCCAT ATCATCTGTA CTACGGCACC 
GGCTCCGGAT CCTACCAGTT TTCCATGATC CCGACCGGCG GCTCTGGATC AGGGGGCGAC 
CGATCACCCA CCAGAATGCT GACGTCCTGT ACAGCTGCTG GCGGAGCGGC AGGAAACACC 
GGAAACAACA ACCTGATCAA TGCCAACCTC GGGAACCAAA GCGATGGAGT TGATGCTGAC 
GGAAGTCATA GTAATTCACC AACAGCCATG AGCGCATCCA CACGCATAGA TGAGTCTGTC 
TGGAGGCCGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571679.2
CDS	60..1334
Translation	MHIPVDPSTSRRFTPPSASFPCAKVTESQSMSAPGPLRSGRPVESRSVVDVLADHAGELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGDVPDGTLVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTGPPQVATYHRAIKVTVDGPREPRRHRVKPDDPHKQFSDRLSDIERFQRASLRMNPGNGTTRPHQTHYSPSSTTQIPGLWPDQIDPPTLKTCKVEDDLRWTSTDLFQQRTSFPSLSPLTAPRFSDSHMHYPSHFTYSANPSSTGIGGLSVAGMPTSSRYHTYLPPPYPGNQNQNSHFQTNSSPYHLYYGTGSGSYQFSMIPTGGSGSGGDRSPTRMLTSCTAAGGAAGNTGNNNLINANLGNQSDGVDADGSHSNSPTAMSASTRIDESVWRPY

Target ORF information:

RefSeq Version	NM_131604.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio runt-related transcription factor 3 (runx3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131604.2

ORF Insert Sequence:

ATGCATATTC CCGTAGACCC GAGCACGAGC AGACGGTTCA CGCCGCCCTC CGCCTCGTTC 
CCCTGCGCTA AAGTGACCGA GAGCCAGAGC ATGAGCGCGC CGGGCCCGCT GCGCTCCGGC 
CGGCCCGTGG AGAGCCGCTC CGTGGTGGAC GTGCTGGCTG ATCACGCCGG AGAGCTGGTG 
CGCACGGACA GCCCAAACTT TCTCTGCTCG GTCCTGCCGT CTCACTGGAG GTGCAACAAA 
ACCCTTCCCG TGGCGTTTAA GGTGGTGGCT CTTGGTGATG TTCCTGACGG GACTCTGGTC 
ACTGTGATGG CGGGAAATGA TGAGAATTAT TCCGCGGAGC TGAGAAACGC GTCTGCCGTC 
ATGAAGAACC AGGTGGCGCG CTTCAACGAC CTGCGCTTCG TGGGCAGGAG CGGCAGAGGA 
AAGAGCTTCA CTTTGACCAT CACTGTGTTC ACTGGACCGC CGCAAGTCGC CACGTACCAC 
CGCGCTATCA AAGTCACCGT TGACGGACCT CGAGAGCCAA GACGTCACCG CGTGAAGCCG 
GATGACCCTC ATAAGCAATT CTCCGACCGT CTGAGTGACA TCGAGCGCTT CCAGAGAGCC 
AGCCTGAGGA TGAACCCTGG AAACGGTACC ACCCGGCCCC ACCAAACCCA TTACAGCCCC 
TCCAGCACCA CACAGATCCC AGGATTATGG CCAGATCAGA TTGATCCTCC AACACTGAAG 
ACGTGCAAAG TGGAAGACGA CTTGCGCTGG ACAAGTACAG ATCTGTTCCA GCAGAGGACG 
TCTTTCCCGT CTCTGTCTCC ACTAACAGCG CCTCGTTTCT CTGACTCGCA CATGCATTAT 
CCGAGCCACT TCACATACTC CGCCAACCCG TCCAGCACTG GCATCGGGGG ACTGAGTGTG 
GCCGGTATGC CCACCTCTAG CCGCTACCAC ACCTACCTGC CCCCTCCCTA CCCGGGCAAC 
CAAAACCAGA ACTCCCACTT CCAGACCAAC TCCTCGCCAT ATCATCTGTA CTACGGCACC 
GGCTCCGGAT CCTACCAGTT TTCCATGATC CCGACCGGCG GCTCTGGATC AGGGGGCGAC 
CGATCACCCA CCAGAATGCT GACGTCCTGT ACAGCTGCTG GCGGAGCGGC AGGAAACACC 
GGAAACAACA ACCTGATCAA TGCCAACCTC GGGAACCAAA GCGATGGAGT TGATGCTGAC 
GGAAGTCATA GTAATTCACC AACAGCCATG AGCGCATCCA CACGCATAGA TGAGTCTGTC 
TGGAGGCCGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

runx3 ( NM_131604.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571679.2 Danio rerio RUNX family transcription factor 3 (runx3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa159840
Clone ID Related Accession (Same CDS sequence)	NM_131604.3
Accession Version	NM_131604.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	runt-related transcription factor 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01083960.1 and CT025744.6. On Apr 12, 2018 this sequence version replaced NM_131604.2. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB043788.1, BC163552.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATATTC CCGTAGACCC GAGCACGAGC AGACGGTTCA CGCCGCCCTC CGCCTCGTTC 
CCGTGCGCTA AAGTGACCGA GAGCCAGAGC ATGAGCGCGC CGGGCCCCCT GCGCTCCGGC 
CGGCCCGTGG AGAGCCGCTC CGTGGTGGAC GTGCTGGCTG ATCACGCCGG AGAGCTGGTG 
CGCACGGACA GCCCAAACTT TCTCTGCTCG GTCCTGCCGT CTCACTGGAG GTGCAACAAA 
ACCCTTCCCG TGGCGTTTAA GGTGGTGGCT CTTGGTGATG TTCCTGACGG GACTCTGGTC 
ACTGTGATGG CGGGAAATGA TGAGAATTAT TCCGCGGAGC TGAGAAACGC GTCTGCCGTC 
ATGAAGAACC AGGTGGCGCG CTTCAACGAC CTGCGCTTCG TGGGCAGGAG CGGCAGAGGA 
AAGAGCTTCA CTTTGACCAT CACTGTGTTC ACTGGACCGC CGCAAGTCGC CACGTACCAC 
CGCGCTATTA AAGTCACCGT TGACGGACCT CGAGAGCCAA GACGTCACCG CGTGAAGCCG 
GATGACCCTC ATAAGCAATT CTCCGACCGT CTGAGTGACA TCGAGCGCTT CCAGAGAGCC 
AGCCTGAGGA TGAACCCTGG AAACGGTACC ACCCGGCCCC ACCAAACCCA TTACAGCCCC 
TCCAGCACCA CACAGATCCC AGGATTATGG CCAGATCAGA TTGATCCTCC AACACTGAAG 
ACGTGCAAAG TGGAAGACGA CTTGCGCTGG ACAAGTACAG ATCTGTTCCA GCAGAGGACG 
TCTTTCCCGT CTCTGTCTCC ACTAACAGCG CCTCGTTTCT CTGACTCGCA CATGCATTAT 
CCGAGCCACT TCACATACTC CGCCAACCCG TCCAGCACTG GCATCGGGGG ACTGAGTGTG 
GCCGGTATGC CCACCTCCAG CCGCTACCAC ACCTACCTGC CCCCTCCCTA CCCGGGCAAC 
CAAAACCAGA ACTCCCACTT CCAGACCAAC TCCTCGCCAT ATCATCTGTA CTACGGCACC 
GGCTCCGGAT CCTACCAGTT TTCCATGATC CCGACCGGCG GCTCTGGATC AGGGGGCGAC 
CGATCACCCA CCAGAATGCT GACGTCCTGT ACAGCTGCTG GCGGAGCGGC AGGAAACACC 
GGAAACAACA ACCTGATCAA TGCCAACCTC GGGAACCAAA GCGATGGAGT TGATGCTGAC 
GGAAGTCATA GTAATTCACC AACAGCCATG AGCGCGTCCA CACGCATAGA TGAGTCTGTC 
TGGAGGCCGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571679.2
CDS	568..1842
Translation	MHIPVDPSTSRRFTPPSASFPCAKVTESQSMSAPGPLRSGRPVESRSVVDVLADHAGELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGDVPDGTLVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTGPPQVATYHRAIKVTVDGPREPRRHRVKPDDPHKQFSDRLSDIERFQRASLRMNPGNGTTRPHQTHYSPSSTTQIPGLWPDQIDPPTLKTCKVEDDLRWTSTDLFQQRTSFPSLSPLTAPRFSDSHMHYPSHFTYSANPSSTGIGGLSVAGMPTSSRYHTYLPPPYPGNQNQNSHFQTNSSPYHLYYGTGSGSYQFSMIPTGGSGSGGDRSPTRMLTSCTAAGGAAGNTGNNNLINANLGNQSDGVDADGSHSNSPTAMSASTRIDESVWRPY

Target ORF information:

RefSeq Version	NM_131604.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RUNX family transcription factor 3 (runx3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131604.3

ORF Insert Sequence:

ATGCATATTC CCGTAGACCC GAGCACGAGC AGACGGTTCA CGCCGCCCTC CGCCTCGTTC 
CCGTGCGCTA AAGTGACCGA GAGCCAGAGC ATGAGCGCGC CGGGCCCCCT GCGCTCCGGC 
CGGCCCGTGG AGAGCCGCTC CGTGGTGGAC GTGCTGGCTG ATCACGCCGG AGAGCTGGTG 
CGCACGGACA GCCCAAACTT TCTCTGCTCG GTCCTGCCGT CTCACTGGAG GTGCAACAAA 
ACCCTTCCCG TGGCGTTTAA GGTGGTGGCT CTTGGTGATG TTCCTGACGG GACTCTGGTC 
ACTGTGATGG CGGGAAATGA TGAGAATTAT TCCGCGGAGC TGAGAAACGC GTCTGCCGTC 
ATGAAGAACC AGGTGGCGCG CTTCAACGAC CTGCGCTTCG TGGGCAGGAG CGGCAGAGGA 
AAGAGCTTCA CTTTGACCAT CACTGTGTTC ACTGGACCGC CGCAAGTCGC CACGTACCAC 
CGCGCTATTA AAGTCACCGT TGACGGACCT CGAGAGCCAA GACGTCACCG CGTGAAGCCG 
GATGACCCTC ATAAGCAATT CTCCGACCGT CTGAGTGACA TCGAGCGCTT CCAGAGAGCC 
AGCCTGAGGA TGAACCCTGG AAACGGTACC ACCCGGCCCC ACCAAACCCA TTACAGCCCC 
TCCAGCACCA CACAGATCCC AGGATTATGG CCAGATCAGA TTGATCCTCC AACACTGAAG 
ACGTGCAAAG TGGAAGACGA CTTGCGCTGG ACAAGTACAG ATCTGTTCCA GCAGAGGACG 
TCTTTCCCGT CTCTGTCTCC ACTAACAGCG CCTCGTTTCT CTGACTCGCA CATGCATTAT 
CCGAGCCACT TCACATACTC CGCCAACCCG TCCAGCACTG GCATCGGGGG ACTGAGTGTG 
GCCGGTATGC CCACCTCCAG CCGCTACCAC ACCTACCTGC CCCCTCCCTA CCCGGGCAAC 
CAAAACCAGA ACTCCCACTT CCAGACCAAC TCCTCGCCAT ATCATCTGTA CTACGGCACC 
GGCTCCGGAT CCTACCAGTT TTCCATGATC CCGACCGGCG GCTCTGGATC AGGGGGCGAC 
CGATCACCCA CCAGAATGCT GACGTCCTGT ACAGCTGCTG GCGGAGCGGC AGGAAACACC 
GGAAACAACA ACCTGATCAA TGCCAACCTC GGGAACCAAA GCGATGGAGT TGATGCTGAC 
GGAAGTCATA GTAATTCACC AACAGCCATG AGCGCGTCCA CACGCATAGA TGAGTCTGTC 
TGGAGGCCGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

runx3 ( NM_001362344.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001349273.1 Danio rerio RUNX family transcription factor 3 (runx3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa159874
Clone ID Related Accession (Same CDS sequence)	NM_001362344.1
Accession Version	NM_001362344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	runt-related transcription factor 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01078998.1, CABZ01083960.1 and CT025744.6. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB043790.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA708836 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCAA ACAGCATCTT CGACACATTC TCTAGCTACT CGCCCAGTTT GCTGCGAGAC 
CCGAGCACGA GCAGACGGTT CACGCCGCCC TCCGCCTCGT TCCCGTGCGC TAAAGTGACC 
GAGAGCCAGA GCATGAGCGC GCCGGGCCCC CTGCGCTCCG GCCGGCCCGT GGAGAGCCGC 
TCCGTGGTGG ACGTGCTGGC TGATCACGCC GGAGAGCTGG TGCGCACGGA CAGCCCAAAC 
TTTCTCTGCT CGGTCCTGCC GTCTCACTGG AGGTGCAACA AAACCCTTCC CGTGGCGTTT 
AAGGTGGTGG CTCTTGGTGA TGTTCCTGAC GGGACTCTGG TCACTGTGAT GGCGGGAAAT 
GATGAGAATT ATTCCGCGGA GCTGAGAAAC GCGTCTGCCG TCATGAAGAA CCAGGTGGCG 
CGCTTCAACG ACCTGCGCTT CGTGGGCAGG AGCGGCAGAG GAAAGAGCTT CACTTTGACC 
ATCACTGTGT TCACTGGACC GCCGCAAGTC GCCACGTACC ACCGCGCTAT TAAAGTCACC 
GTTGACGGAC CTCGAGAGCC AAGACGTCAC CGCGTGAAGC CGGATGACCC TCATAAGCAA 
TTCTCCGACC GTCTGAGTGA CATCGAGCGC TTCCAGAGAG CCAGCCTGAG GATGAACCCT 
GGAAACGGTA CCACCCGGCC CCACCAAACC CATTACAGCC CCTCCAGCAC CACACAGATC 
CCAGGATTAT GGCCAGATCA GATTGATCCT CCAACACTGA AGACGTGCAA AGTGGAAGAC 
GACTTGCGCT GGACAAGTAC AGATCTGTTC CAGCAGAGGA CGTCTTTCCC GTCTCTGTCT 
CCACTAACAG CGCCTCGTTT CTCTGACTCG CACATGCATT ATCCGAGCCA CTTCACATAC 
TCCGCCAACC CGTCCAGCAC TGGCATCGGG GGACTGAGTG TGGCCGGTAT GCCCACCTCC 
AGCCGCTACC ACACCTACCT GCCCCCTCCC TACCCGGGCA ACCAAAACCA GAACTCCCAC 
TTCCAGACCA ACTCCTCGCC ATATCATCTG TACTACGGCA CCGGCTCCGG ATCCTACCAG 
TTTTCCATGA TCCCGACCGG CGGCTCTGGA TCAGGGGGCG ACCGATCACC CACCAGAATG 
CTGACGTCCT GTACAGCTGC TGGCGGAGCG GCAGGAAACA CCGGAAACAA CAACCTGATC 
AATGCCAACC TCGGGAACCA AAGCGATGGA GTTGATGCTG ACGGAAGTCA TAGTAATTCA 
CCAACAGCCA TGAGCGCGTC CACACGCATA GATGAGTCTG TCTGGAGGCC GTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349273.1
CDS	131..1447
Translation	MASNSIFDTFSSYSPSLLRDPSTSRRFTPPSASFPCAKVTESQSMSAPGPLRSGRPVESRSVVDVLADHAGELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGDVPDGTLVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTGPPQVATYHRAIKVTVDGPREPRRHRVKPDDPHKQFSDRLSDIERFQRASLRMNPGNGTTRPHQTHYSPSSTTQIPGLWPDQIDPPTLKTCKVEDDLRWTSTDLFQQRTSFPSLSPLTAPRFSDSHMHYPSHFTYSANPSSTGIGGLSVAGMPTSSRYHTYLPPPYPGNQNQNSHFQTNSSPYHLYYGTGSGSYQFSMIPTGGSGSGGDRSPTRMLTSCTAAGGAAGNTGNNNLINANLGNQSDGVDADGSHSNSPTAMSASTRIDESVWRPY

Target ORF information:

RefSeq Version	NM_001362344.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RUNX family transcription factor 3 (runx3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362344.1

ORF Insert Sequence:

ATGGCTTCAA ACAGCATCTT CGACACATTC TCTAGCTACT CGCCCAGTTT GCTGCGAGAC 
CCGAGCACGA GCAGACGGTT CACGCCGCCC TCCGCCTCGT TCCCGTGCGC TAAAGTGACC 
GAGAGCCAGA GCATGAGCGC GCCGGGCCCC CTGCGCTCCG GCCGGCCCGT GGAGAGCCGC 
TCCGTGGTGG ACGTGCTGGC TGATCACGCC GGAGAGCTGG TGCGCACGGA CAGCCCAAAC 
TTTCTCTGCT CGGTCCTGCC GTCTCACTGG AGGTGCAACA AAACCCTTCC CGTGGCGTTT 
AAGGTGGTGG CTCTTGGTGA TGTTCCTGAC GGGACTCTGG TCACTGTGAT GGCGGGAAAT 
GATGAGAATT ATTCCGCGGA GCTGAGAAAC GCGTCTGCCG TCATGAAGAA CCAGGTGGCG 
CGCTTCAACG ACCTGCGCTT CGTGGGCAGG AGCGGCAGAG GAAAGAGCTT CACTTTGACC 
ATCACTGTGT TCACTGGACC GCCGCAAGTC GCCACGTACC ACCGCGCTAT TAAAGTCACC 
GTTGACGGAC CTCGAGAGCC AAGACGTCAC CGCGTGAAGC CGGATGACCC TCATAAGCAA 
TTCTCCGACC GTCTGAGTGA CATCGAGCGC TTCCAGAGAG CCAGCCTGAG GATGAACCCT 
GGAAACGGTA CCACCCGGCC CCACCAAACC CATTACAGCC CCTCCAGCAC CACACAGATC 
CCAGGATTAT GGCCAGATCA GATTGATCCT CCAACACTGA AGACGTGCAA AGTGGAAGAC 
GACTTGCGCT GGACAAGTAC AGATCTGTTC CAGCAGAGGA CGTCTTTCCC GTCTCTGTCT 
CCACTAACAG CGCCTCGTTT CTCTGACTCG CACATGCATT ATCCGAGCCA CTTCACATAC 
TCCGCCAACC CGTCCAGCAC TGGCATCGGG GGACTGAGTG TGGCCGGTAT GCCCACCTCC 
AGCCGCTACC ACACCTACCT GCCCCCTCCC TACCCGGGCA ACCAAAACCA GAACTCCCAC 
TTCCAGACCA ACTCCTCGCC ATATCATCTG TACTACGGCA CCGGCTCCGG ATCCTACCAG 
TTTTCCATGA TCCCGACCGG CGGCTCTGGA TCAGGGGGCG ACCGATCACC CACCAGAATG 
CTGACGTCCT GTACAGCTGC TGGCGGAGCG GCAGGAAACA CCGGAAACAA CAACCTGATC 
AATGCCAACC TCGGGAACCA AAGCGATGGA GTTGATGCTG ACGGAAGTCA TAGTAATTCA 
CCAACAGCCA TGAGCGCGTC CACACGCATA GATGAGTCTG TCTGGAGGCC GTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
The Journal of experimental medicine214(10)2875-2887(2017 Oct)
Tang Q,Iyer S,Lobbardi R,Moore JC,Chen H,Lareau C,Hebert C,Shaw ML,Neftel C,Suva ML,Ceol CJ,Bernards A,Aryee M,Pinello L,Drummond IA,Langenau DM

An ancient neurotrophin receptor code; a single Runx/Cbf? complex determines somatosensory neuron fate specification in zebrafish.
PLoS genetics13(7)e1006884(2017 Jul)
Gau P,Curtright A,Condon L,Raible DW,Dhaka A

Evolutionary and Expression Analyses Show Co-option of
Frontiers in genetics8225(2017)
Wang S,Yang Q,Wang Z,Feng S,Li H,Ji D,Zhang S

CD4-Transgenic Zebrafish Reveal Tissue-Resident Th2- and Regulatory T Cell-like Populations and Diverse Mononuclear Phagocytes.
Journal of immunology (Baltimore, Md. : 1950)197(9)3520-3530(2016 Nov)
Dee CT,Nagaraju RT,Athanasiadis EI,Gray C,Fernandez Del Ama L,Johnston SA,Secombes CJ,Cvejic A,Hurlstone AF

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runx3 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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