spon1b cDNA ORF clone, Danio rerio(zebrafish)

The following spon1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spon1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa32413 NM_131517.1
Latest version!
Danio rerio spondin 1b (spon1b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa29288 XM_005174346.4
Latest version!
Danio rerio spondin 1b (spon1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
ODa29288 XM_005174346.3 Danio rerio spondin 1b (spon1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa32413
    Clone ID Related Accession (Same CDS sequence) NM_131517.1
    Accession Version NM_131517.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-23
    Organism Danio rerio(zebrafish)
    Product spondin 1b precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB006087.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB006087.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATGGACA AGGACGGGCT GGTTGTGCTG CTAGTTTTCT TAAACGTGTT TTTGATTCAC 
    TCGGATGCGT TCAAAAGAGA TACCGCGGAG AGACATTCGA GATCTGAGGG GGGTTTCTGT
    CGGATATTGC GGACGCAGGC GCGGAGCGCT CGGCGAGACG GACAGAACGA GTTTCGCCTC
    AAAGTTGAAG GAGAGCCAGA AACTTACCAA CCCGGGAGCA CCTACAGAGT GAGTTTGTAT
    GCCTCCAGCC CATCATACTT TCGTGGTTTT ACCCTCATTG CTCTGAAGGA AGGAAGGGAG
    GGCGTGAGTC CTGACGATTA TGCTGGCACT TTCAAGCTCA TTGATGAAAG CGACACACAG
    TTCATGACCA ACTGTCCTCC AGCCGTCACA GAGAGCATTC CCAGGAGACG CACGCGGATA
    CAGGTGTTTT GGACAGCACC ACCTTCTGGC AGCGGCTGCG TCACTCTCAA GGCCAGTGTT
    GTGCAGCGAA GAATCATTTT CTTCCAAGAC GAGGGCTCGC TTACCAAAAG ATTGTGCGAG
    AAAGATCCTG TTGCGATTAC TGAAAAACCT GCTCAAGAGT GTTGTGCTTG TGGAACAGCC
    AAGTATAGAG TGACTTTCTA TGGAAACTGG TCGGAGAAGC TCGATCCCAA AGATTACCCA
    AGACGTGCTA ATCATTGGTC GGCTCTCATT GGCGCGTCTC ACACTAGAAG TTATGTGTTA
    TGGGAATATG GAGGATTTGC CAGTGAAGGA GTCAAGCAGG TGTCCGAGTA TGGATCACCC
    GTCAAAATGG AGGAGGAGAT CCGACAAAAG GGTGATGATG TTATGACAGT TATCAAAACC
    AAAGCCCAGT GGCCCGCCTG GCAGCCTCTT AATATTCGCG CCGCCCCATC AGCTGAGTTT
    TCGGTGGATC GAGTCCGACA CCTGATCTCC TTTTTGACCA TGCTGGGCCC CAGTCCTGAC
    TGGAACGTGG GTTTGTCTGG AGAAGATCTC TGCACCAGGG ATTGTGGCTG GGTCCAAAAA
    CTGGTGAAGG ATCTGGTCCC TTGGGATGCT GGGACTGATA GCGGTGTCTC TTATGAGTCT
    CCCAATAAAC CCAGCATTCC ACAGGAGCGC ATTCGACCCC TGACCAGCTT GGATCACCCA
    CAAAGCCCTT TTTATGACCC ATCAGGTGGT CCCATGACAC CACTCGGCCG AGTGGTGGTG
    GAGAGAATTG CAAGAAAGGG AGAGCAGTGC AATACCGTGC CCGACACGAT AGACGATATC
    GTCGCTGATA TTGCTCAGGA GGAGAAGGAG GAAGGAGATA ACACTCCAGA GACGTGCATT
    TACTCCAACT GGTCTCCGTG GTCGGCCTGC AGCTCCTCCA CCTGTGATAA GGGGCGGAGG
    ATGAGGCAGA GGATGCTAAA AGCGCAGCTG GACCCCAACC TGCCATGTCT GCACACCCAA
    GACTTTGAGC CCTGTATGGG GCCGGGCTGC AGCGAGGAGG AGGCCTCTAC TTGTATGATG
    TCTGAGTGGA TCAGCTGGTC ACCCTGCAGC GCTTCATGCG GGATGGGCAT GAGGTCCAGA
    GAGAGGTACG TCAAGCAGTT CCCAGAGGAC GGATCCAGCT GCACACTGCC AACAGAGGAG
    ACAGAGAAGT GTGTGGTCAA TGACGACTGC TCACCCAGTA GCTGTGTGGT GACGGAGTGG
    GGCGAGTGGG AGCCCTGTAG TGTAAGCTGT GGTTTGGGCA TGAGGAGGAG AGAACGCATG
    ATGAAGATGG ACGCCTCGGA CGGTTCGCCC TGTCGAGTGC AGATGGCAGA GGCAGAGAAG
    TGCATGATGC CCGAGTGCAA TGCGGTGGTG TGTATGCTGT CGCCATGGTC AGACTGGGGT
    GAGTGCAGTG TGTCCTGCGG TGTGGGCATG AGAACCCGTC AGCGCATGCT AAAGACACCC
    GTTAAGCCCG GCCAGTGTCC AGATGAGCTG GAGCAGGTGG AGAAGTGCAT GCTTCCCGAA
    TGCCCTACAG ACTGCATGCT GTCAGAGTGG TCAGCCTGGT CCGAGTGCAA CAAGTCCTGT
    GGGAAGGGGC ACATGATCCG CTCTCGCATG GTCAAGCTGG AGCCTCAGTT TGGAGGTCTG
    CCCTGTCCTG AGACGGTCCA GAGGAAGAAA TGCAAGATCC GCAAGTGCTC GAGAGGCTCC
    CGGGCCAGCG AGAAGAGGAA GAGACAGGAG GTTTCAGACA GGAGGAGAGC CAAGCAGAGC
    CGGGAGTCTG TGGCAGAGGA GAGCACAGTC TGTCCAATAC GCCCTTGGTC CAGCTGGACG
    GAGTGTACTA AACCATGTGA TGGGGGGACT CAAGAGCGAG TGATGACCAT AAAGAAAAAG
    GGCAAATCCT CACAGCACAA CCACTGCAAA AACAAGAAGG AGATCCGTGC GTGCAACGTA
    CACCCATGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_571592.1
    CDS180..2591
    Translation

    Target ORF information:

    RefSeq Version NM_131517.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spondin 1b (spon1b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_131517.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATGGACA AGGACGGGCT GGTTGTGCTG CTAGTTTTCT TAAACGTGTT TTTGATTCAC 
    TCGGATGCGT TCAAAAGAGA TACCGCGGAG AGACATTCGA GATCTGAGGG GGGTTTCTGT
    CGGATATTGC GGACGCAGGC GCGGAGCGCT CGGCGAGACG GACAGAACGA GTTTCGCCTC
    AAAGTTGAAG GAGAGCCAGA AACTTACCAA CCCGGGAGCA CCTACAGAGT GAGTTTGTAT
    GCCTCCAGCC CATCATACTT TCGTGGTTTT ACCCTCATTG CTCTGAAGGA AGGAAGGGAG
    GGCGTGAGTC CTGACGATTA TGCTGGCACT TTCAAGCTCA TTGATGAAAG CGACACACAG
    TTCATGACCA ACTGTCCTCC AGCCGTCACA GAGAGCATTC CCAGGAGACG CACGCGGATA
    CAGGTGTTTT GGACAGCACC ACCTTCTGGC AGCGGCTGCG TCACTCTCAA GGCCAGTGTT
    GTGCAGCGAA GAATCATTTT CTTCCAAGAC GAGGGCTCGC TTACCAAAAG ATTGTGCGAG
    AAAGATCCTG TTGCGATTAC TGAAAAACCT GCTCAAGAGT GTTGTGCTTG TGGAACAGCC
    AAGTATAGAG TGACTTTCTA TGGAAACTGG TCGGAGAAGC TCGATCCCAA AGATTACCCA
    AGACGTGCTA ATCATTGGTC GGCTCTCATT GGCGCGTCTC ACACTAGAAG TTATGTGTTA
    TGGGAATATG GAGGATTTGC CAGTGAAGGA GTCAAGCAGG TGTCCGAGTA TGGATCACCC
    GTCAAAATGG AGGAGGAGAT CCGACAAAAG GGTGATGATG TTATGACAGT TATCAAAACC
    AAAGCCCAGT GGCCCGCCTG GCAGCCTCTT AATATTCGCG CCGCCCCATC AGCTGAGTTT
    TCGGTGGATC GAGTCCGACA CCTGATCTCC TTTTTGACCA TGCTGGGCCC CAGTCCTGAC
    TGGAACGTGG GTTTGTCTGG AGAAGATCTC TGCACCAGGG ATTGTGGCTG GGTCCAAAAA
    CTGGTGAAGG ATCTGGTCCC TTGGGATGCT GGGACTGATA GCGGTGTCTC TTATGAGTCT
    CCCAATAAAC CCAGCATTCC ACAGGAGCGC ATTCGACCCC TGACCAGCTT GGATCACCCA
    CAAAGCCCTT TTTATGACCC ATCAGGTGGT CCCATGACAC CACTCGGCCG AGTGGTGGTG
    GAGAGAATTG CAAGAAAGGG AGAGCAGTGC AATACCGTGC CCGACACGAT AGACGATATC
    GTCGCTGATA TTGCTCAGGA GGAGAAGGAG GAAGGAGATA ACACTCCAGA GACGTGCATT
    TACTCCAACT GGTCTCCGTG GTCGGCCTGC AGCTCCTCCA CCTGTGATAA GGGGCGGAGG
    ATGAGGCAGA GGATGCTAAA AGCGCAGCTG GACCCCAACC TGCCATGTCT GCACACCCAA
    GACTTTGAGC CCTGTATGGG GCCGGGCTGC AGCGAGGAGG AGGCCTCTAC TTGTATGATG
    TCTGAGTGGA TCAGCTGGTC ACCCTGCAGC GCTTCATGCG GGATGGGCAT GAGGTCCAGA
    GAGAGGTACG TCAAGCAGTT CCCAGAGGAC GGATCCAGCT GCACACTGCC AACAGAGGAG
    ACAGAGAAGT GTGTGGTCAA TGACGACTGC TCACCCAGTA GCTGTGTGGT GACGGAGTGG
    GGCGAGTGGG AGCCCTGTAG TGTAAGCTGT GGTTTGGGCA TGAGGAGGAG AGAACGCATG
    ATGAAGATGG ACGCCTCGGA CGGTTCGCCC TGTCGAGTGC AGATGGCAGA GGCAGAGAAG
    TGCATGATGC CCGAGTGCAA TGCGGTGGTG TGTATGCTGT CGCCATGGTC AGACTGGGGT
    GAGTGCAGTG TGTCCTGCGG TGTGGGCATG AGAACCCGTC AGCGCATGCT AAAGACACCC
    GTTAAGCCCG GCCAGTGTCC AGATGAGCTG GAGCAGGTGG AGAAGTGCAT GCTTCCCGAA
    TGCCCTACAG ACTGCATGCT GTCAGAGTGG TCAGCCTGGT CCGAGTGCAA CAAGTCCTGT
    GGGAAGGGGC ACATGATCCG CTCTCGCATG GTCAAGCTGG AGCCTCAGTT TGGAGGTCTG
    CCCTGTCCTG AGACGGTCCA GAGGAAGAAA TGCAAGATCC GCAAGTGCTC GAGAGGCTCC
    CGGGCCAGCG AGAAGAGGAA GAGACAGGAG GTTTCAGACA GGAGGAGAGC CAAGCAGAGC
    CGGGAGTCTG TGGCAGAGGA GAGCACAGTC TGTCCAATAC GCCCTTGGTC CAGCTGGACG
    GAGTGTACTA AACCATGTGA TGGGGGGACT CAAGAGCGAG TGATGACCAT AAAGAAAAAG
    GGCAAATCCT CACAGCACAA CCACTGCAAA AACAAGAAGG AGATCCGTGC GTGCAACGTA
    CACCCATGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa29288
    Clone ID Related Accession (Same CDS sequence) XM_005174346.3 , XM_005174346.4
    Accession Version XM_005174346.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spondin 1b isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174346.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATGGACA AGGACGGGCT GGTTGTGCTG CTAGTTTTCT TAAACGTGTT TTTGATTCAC 
    TCGGATGCGT TCAAAAGAGA TACCGCGGAG AGACATTCGA GATCTGAGGG GGGTTTCTGT
    CGGATATTGC GGACGCAGGC GCGGAGCGCT CGGCGAGACG GACAGAACGA GTTTCGCCTC
    AAAGTTGAAG GAGAGCCAGA AACTTACCAA CCCGGGAGCA CCTACAGAGT GAGTTTGTAT
    GCCTCCAGCC CATCATACTT TCGTGGTTTT ACCCTCATTG CTCTGAAGGA AGGAAGGGAG
    GGCGTGAGTC CTGACGATTA TGCTGGCACT TTCAAGCTCA TTGATGAAAG CGACACACAG
    TTCATGACCA ACTGTCCTCC AGCCGTCACA GAGAGCATTC CCAGGAGACG CACGCGGATA
    CAGGTGTTTT GGACAGCACC ACCTTCTGGC AGCGGCTGCG TCACTCTCAA GGCCAGTGTT
    GTGCAGCGAA GAATCATTTT CTTCCAAGAC GAGGGCTCGC TTACCAAAAG ATTGTGCGAG
    AAAGATCCTG TTGCGATTAC TGAAAAACCT GCTCAAGAGT GTTGTGCTTG TGGAACAGCC
    AAGTATAGAG TGACTTTCTA TGGAAACTGG TCGGAGAAGC TCCATCCCAA AGATTACCCA
    AGACGTGCTA ATCATTGGTC GGCTCTCATT GGCGCGTCTC ACACTAGAAG TTATGTGTTA
    TGGGAATATG GAGGATTTGC CAGTGAAGGA GTCAAGCAGG TGTCCGAGTA TGGATCACCC
    GTCAAAATGG AGGAGGAGAT CCGACAAAAG GGTGATGATG TTATGACAGT TATCAAAACC
    AAAGCCCAGT GGCCCGCCTG GCAGCCTCTT AATATTCGCG CCGCCCCATC AGCTGAGTTT
    TCGGTGGATC GAGTCCGACA CCTGATGTCC TTTTTGACCA TGCTGGGCCC CAGTCCTGAC
    TGGAACGTGG GTTTGTCTGG AGAAGATCTC TGCACCAGGG ATTGTGGCTG GGTCCAAAAA
    CTGGTGAAGG ATCTGGTCCC TTGGGATGCT GGGACTGATA GCGGTGTCTC TTATGAGTCT
    CCCAATAAAC CCAGCATTCC ACAGGAGCGC ATTCGACCCC TGACCAGCTT GGATCACCCA
    CAAAGCCCTT TTTATGACCC ATCAGGTGGT CCCATGACAC CACTCGGCCG AGTGGTGGTG
    GAGAGAATTG CAAGAAAGGG AGAGCAGTGC AATACCGTGC CCGACACGAT AGACGATATC
    GTCGCTGATA TTGCTCAGGA GGAGAAGGAG GAAGGAGATA ACACTCCAGA GACGTGCATT
    TACTCCAACT GGTCTCCGTG GTCGGCCTGC AGCTCCTCCA CCTGTGATAA GGGGCGGAGG
    ATGAGGCAGA GGATGCTAAA AGCGCAGCTG GACCCCAACC TGCCATGTCT GCACACCCAA
    GACTTTGAGC CCTGTATGGG GCCGGGCTGC AGCGAGGAGG AGGCCTCTAC TTGTATGATG
    TCTGAGTGGA TCAGCTGGTC ACCCTGCAGC GCTTCATGCG GGATGGGCAT GAGGTCCAGA
    GAGAGGTACG TCAAGCAGTT CCCAGAGGAC GGATCCAGCT GCACACTGCC AACAGAGGAG
    ACAGAGAAGT GTGTGGTCAA TGACGACTGC TCACCCAGTA GCTGTGTGGT GACGGAGTGG
    GGCGAGTGGG AGCCCTGTAG TGTAAGCTGT GGTTTGGGCA TGAGGAGGAG AGAACGCATG
    ATGAAGATGG ACGCCTCGGA CGGTTCGCCC TGTCGAGTGC AGATGGCAGA GGCAGAGAAG
    TGCATGATGC CCGAGTGCAA TGCGGTGGTG TGTATGCTGT CGCCATGGTC AGACTGGGGT
    GAGTGCAGTG TGTCCTGCGG TGTGGGCATG AGAACCCGTC AGCGCATGCT AAAGACACCC
    GTTAAGCCCG GCCAGTGTCC AGATGAGCTG GAGCAGGTGG AGAAGTGCAT GCTTCCCGAA
    TGCCCTACAG ACTGCATGCT GTCAGAGTGG TCAGCCTGGT CCGAGTGCAA CAAGTCCTGT
    GGGAAGGGGC ACATGATCCG CTCTCGCATG GTCAAGCTGG AGCCTCAGTT TGGAGGTCTG
    CCCTGTCCTG AGACGGTCCA GAGGAAGAAA TGCAAGATCC GCAAGTGCTC GAGAGGCTCC
    CGGGCCAGCG AGAAGAGGAA GAGACAGGAG GTTTCAGACA GGAGGAGAGC CAAGCAGAGC
    CGGGAGTCTG TGGCAGAGGA GAGCACAGTA GTCTGTCCAA TACGCCCTTG GTCCAGCTGG
    ACGGAGTGTA CTAAACCATG TGATGGGGGG ACTCAAGAGC GAGTGATGAC CATAAAGAAA
    AAGGGCAAAT CCTCACAGCA CAACCACTGC AAAAACAAGA AGGAGATCCG TGCGTGCAAC
    GTACACCCAT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005174403.1
    CDS479..2893
    Translation

    Target ORF information:

    RefSeq Version XM_005174346.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spondin 1b (spon1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005174346.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATGGACA AGGACGGGCT GGTTGTGCTG CTAGTTTTCT TAAACGTGTT TTTGATTCAC 
    TCGGATGCGT TCAAAAGAGA TACCGCGGAG AGACATTCGA GATCTGAGGG GGGTTTCTGT
    CGGATATTGC GGACGCAGGC GCGGAGCGCT CGGCGAGACG GACAGAACGA GTTTCGCCTC
    AAAGTTGAAG GAGAGCCAGA AACTTACCAA CCCGGGAGCA CCTACAGAGT GAGTTTGTAT
    GCCTCCAGCC CATCATACTT TCGTGGTTTT ACCCTCATTG CTCTGAAGGA AGGAAGGGAG
    GGCGTGAGTC CTGACGATTA TGCTGGCACT TTCAAGCTCA TTGATGAAAG CGACACACAG
    TTCATGACCA ACTGTCCTCC AGCCGTCACA GAGAGCATTC CCAGGAGACG CACGCGGATA
    CAGGTGTTTT GGACAGCACC ACCTTCTGGC AGCGGCTGCG TCACTCTCAA GGCCAGTGTT
    GTGCAGCGAA GAATCATTTT CTTCCAAGAC GAGGGCTCGC TTACCAAAAG ATTGTGCGAG
    AAAGATCCTG TTGCGATTAC TGAAAAACCT GCTCAAGAGT GTTGTGCTTG TGGAACAGCC
    AAGTATAGAG TGACTTTCTA TGGAAACTGG TCGGAGAAGC TCCATCCCAA AGATTACCCA
    AGACGTGCTA ATCATTGGTC GGCTCTCATT GGCGCGTCTC ACACTAGAAG TTATGTGTTA
    TGGGAATATG GAGGATTTGC CAGTGAAGGA GTCAAGCAGG TGTCCGAGTA TGGATCACCC
    GTCAAAATGG AGGAGGAGAT CCGACAAAAG GGTGATGATG TTATGACAGT TATCAAAACC
    AAAGCCCAGT GGCCCGCCTG GCAGCCTCTT AATATTCGCG CCGCCCCATC AGCTGAGTTT
    TCGGTGGATC GAGTCCGACA CCTGATGTCC TTTTTGACCA TGCTGGGCCC CAGTCCTGAC
    TGGAACGTGG GTTTGTCTGG AGAAGATCTC TGCACCAGGG ATTGTGGCTG GGTCCAAAAA
    CTGGTGAAGG ATCTGGTCCC TTGGGATGCT GGGACTGATA GCGGTGTCTC TTATGAGTCT
    CCCAATAAAC CCAGCATTCC ACAGGAGCGC ATTCGACCCC TGACCAGCTT GGATCACCCA
    CAAAGCCCTT TTTATGACCC ATCAGGTGGT CCCATGACAC CACTCGGCCG AGTGGTGGTG
    GAGAGAATTG CAAGAAAGGG AGAGCAGTGC AATACCGTGC CCGACACGAT AGACGATATC
    GTCGCTGATA TTGCTCAGGA GGAGAAGGAG GAAGGAGATA ACACTCCAGA GACGTGCATT
    TACTCCAACT GGTCTCCGTG GTCGGCCTGC AGCTCCTCCA CCTGTGATAA GGGGCGGAGG
    ATGAGGCAGA GGATGCTAAA AGCGCAGCTG GACCCCAACC TGCCATGTCT GCACACCCAA
    GACTTTGAGC CCTGTATGGG GCCGGGCTGC AGCGAGGAGG AGGCCTCTAC TTGTATGATG
    TCTGAGTGGA TCAGCTGGTC ACCCTGCAGC GCTTCATGCG GGATGGGCAT GAGGTCCAGA
    GAGAGGTACG TCAAGCAGTT CCCAGAGGAC GGATCCAGCT GCACACTGCC AACAGAGGAG
    ACAGAGAAGT GTGTGGTCAA TGACGACTGC TCACCCAGTA GCTGTGTGGT GACGGAGTGG
    GGCGAGTGGG AGCCCTGTAG TGTAAGCTGT GGTTTGGGCA TGAGGAGGAG AGAACGCATG
    ATGAAGATGG ACGCCTCGGA CGGTTCGCCC TGTCGAGTGC AGATGGCAGA GGCAGAGAAG
    TGCATGATGC CCGAGTGCAA TGCGGTGGTG TGTATGCTGT CGCCATGGTC AGACTGGGGT
    GAGTGCAGTG TGTCCTGCGG TGTGGGCATG AGAACCCGTC AGCGCATGCT AAAGACACCC
    GTTAAGCCCG GCCAGTGTCC AGATGAGCTG GAGCAGGTGG AGAAGTGCAT GCTTCCCGAA
    TGCCCTACAG ACTGCATGCT GTCAGAGTGG TCAGCCTGGT CCGAGTGCAA CAAGTCCTGT
    GGGAAGGGGC ACATGATCCG CTCTCGCATG GTCAAGCTGG AGCCTCAGTT TGGAGGTCTG
    CCCTGTCCTG AGACGGTCCA GAGGAAGAAA TGCAAGATCC GCAAGTGCTC GAGAGGCTCC
    CGGGCCAGCG AGAAGAGGAA GAGACAGGAG GTTTCAGACA GGAGGAGAGC CAAGCAGAGC
    CGGGAGTCTG TGGCAGAGGA GAGCACAGTA GTCTGTCCAA TACGCCCTTG GTCCAGCTGG
    ACGGAGTGTA CTAAACCATG TGATGGGGGG ACTCAAGAGC GAGTGATGAC CATAAAGAAA
    AAGGGCAAAT CCTCACAGCA CAACCACTGC AAAAACAAGA AGGAGATCCG TGCGTGCAAC
    GTACACCCAT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa29288
    Clone ID Related Accession (Same CDS sequence) XM_005174346.3 , XM_005174346.4
    Accession Version XM_005174346.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spondin 1b isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174346.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATGGACA AGGACGGGCT GGTTGTGCTG CTAGTTTTCT TAAACGTGTT TTTGATTCAC 
    TCGGATGCGT TCAAAAGAGA TACCGCGGAG AGACATTCGA GATCTGAGGG GGGTTTCTGT
    CGGATATTGC GGACGCAGGC GCGGAGCGCT CGGCGAGACG GACAGAACGA GTTTCGCCTC
    AAAGTTGAAG GAGAGCCAGA AACTTACCAA CCCGGGAGCA CCTACAGAGT GAGTTTGTAT
    GCCTCCAGCC CATCATACTT TCGTGGTTTT ACCCTCATTG CTCTGAAGGA AGGAAGGGAG
    GGCGTGAGTC CTGACGATTA TGCTGGCACT TTCAAGCTCA TTGATGAAAG CGACACACAG
    TTCATGACCA ACTGTCCTCC AGCCGTCACA GAGAGCATTC CCAGGAGACG CACGCGGATA
    CAGGTGTTTT GGACAGCACC ACCTTCTGGC AGCGGCTGCG TCACTCTCAA GGCCAGTGTT
    GTGCAGCGAA GAATCATTTT CTTCCAAGAC GAGGGCTCGC TTACCAAAAG ATTGTGCGAG
    AAAGATCCTG TTGCGATTAC TGAAAAACCT GCTCAAGAGT GTTGTGCTTG TGGAACAGCC
    AAGTATAGAG TGACTTTCTA TGGAAACTGG TCGGAGAAGC TCCATCCCAA AGATTACCCA
    AGACGTGCTA ATCATTGGTC GGCTCTCATT GGCGCGTCTC ACACTAGAAG TTATGTGTTA
    TGGGAATATG GAGGATTTGC CAGTGAAGGA GTCAAGCAGG TGTCCGAGTA TGGATCACCC
    GTCAAAATGG AGGAGGAGAT CCGACAAAAG GGTGATGATG TTATGACAGT TATCAAAACC
    AAAGCCCAGT GGCCCGCCTG GCAGCCTCTT AATATTCGCG CCGCCCCATC AGCTGAGTTT
    TCGGTGGATC GAGTCCGACA CCTGATGTCC TTTTTGACCA TGCTGGGCCC CAGTCCTGAC
    TGGAACGTGG GTTTGTCTGG AGAAGATCTC TGCACCAGGG ATTGTGGCTG GGTCCAAAAA
    CTGGTGAAGG ATCTGGTCCC TTGGGATGCT GGGACTGATA GCGGTGTCTC TTATGAGTCT
    CCCAATAAAC CCAGCATTCC ACAGGAGCGC ATTCGACCCC TGACCAGCTT GGATCACCCA
    CAAAGCCCTT TTTATGACCC ATCAGGTGGT CCCATGACAC CACTCGGCCG AGTGGTGGTG
    GAGAGAATTG CAAGAAAGGG AGAGCAGTGC AATACCGTGC CCGACACGAT AGACGATATC
    GTCGCTGATA TTGCTCAGGA GGAGAAGGAG GAAGGAGATA ACACTCCAGA GACGTGCATT
    TACTCCAACT GGTCTCCGTG GTCGGCCTGC AGCTCCTCCA CCTGTGATAA GGGGCGGAGG
    ATGAGGCAGA GGATGCTAAA AGCGCAGCTG GACCCCAACC TGCCATGTCT GCACACCCAA
    GACTTTGAGC CCTGTATGGG GCCGGGCTGC AGCGAGGAGG AGGCCTCTAC TTGTATGATG
    TCTGAGTGGA TCAGCTGGTC ACCCTGCAGC GCTTCATGCG GGATGGGCAT GAGGTCCAGA
    GAGAGGTACG TCAAGCAGTT CCCAGAGGAC GGATCCAGCT GCACACTGCC AACAGAGGAG
    ACAGAGAAGT GTGTGGTCAA TGACGACTGC TCACCCAGTA GCTGTGTGGT GACGGAGTGG
    GGCGAGTGGG AGCCCTGTAG TGTAAGCTGT GGTTTGGGCA TGAGGAGGAG AGAACGCATG
    ATGAAGATGG ACGCCTCGGA CGGTTCGCCC TGTCGAGTGC AGATGGCAGA GGCAGAGAAG
    TGCATGATGC CCGAGTGCAA TGCGGTGGTG TGTATGCTGT CGCCATGGTC AGACTGGGGT
    GAGTGCAGTG TGTCCTGCGG TGTGGGCATG AGAACCCGTC AGCGCATGCT AAAGACACCC
    GTTAAGCCCG GCCAGTGTCC AGATGAGCTG GAGCAGGTGG AGAAGTGCAT GCTTCCCGAA
    TGCCCTACAG ACTGCATGCT GTCAGAGTGG TCAGCCTGGT CCGAGTGCAA CAAGTCCTGT
    GGGAAGGGGC ACATGATCCG CTCTCGCATG GTCAAGCTGG AGCCTCAGTT TGGAGGTCTG
    CCCTGTCCTG AGACGGTCCA GAGGAAGAAA TGCAAGATCC GCAAGTGCTC GAGAGGCTCC
    CGGGCCAGCG AGAAGAGGAA GAGACAGGAG GTTTCAGACA GGAGGAGAGC CAAGCAGAGC
    CGGGAGTCTG TGGCAGAGGA GAGCACAGTA GTCTGTCCAA TACGCCCTTG GTCCAGCTGG
    ACGGAGTGTA CTAAACCATG TGATGGGGGG ACTCAAGAGC GAGTGATGAC CATAAAGAAA
    AAGGGCAAAT CCTCACAGCA CAACCACTGC AAAAACAAGA AGGAGATCCG TGCGTGCAAC
    GTACACCCAT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005174403.1
    CDS476..2890
    Translation

    Target ORF information:

    RefSeq Version XM_005174346.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spondin 1b (spon1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005174346.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATGGACA AGGACGGGCT GGTTGTGCTG CTAGTTTTCT TAAACGTGTT TTTGATTCAC 
    TCGGATGCGT TCAAAAGAGA TACCGCGGAG AGACATTCGA GATCTGAGGG GGGTTTCTGT
    CGGATATTGC GGACGCAGGC GCGGAGCGCT CGGCGAGACG GACAGAACGA GTTTCGCCTC
    AAAGTTGAAG GAGAGCCAGA AACTTACCAA CCCGGGAGCA CCTACAGAGT GAGTTTGTAT
    GCCTCCAGCC CATCATACTT TCGTGGTTTT ACCCTCATTG CTCTGAAGGA AGGAAGGGAG
    GGCGTGAGTC CTGACGATTA TGCTGGCACT TTCAAGCTCA TTGATGAAAG CGACACACAG
    TTCATGACCA ACTGTCCTCC AGCCGTCACA GAGAGCATTC CCAGGAGACG CACGCGGATA
    CAGGTGTTTT GGACAGCACC ACCTTCTGGC AGCGGCTGCG TCACTCTCAA GGCCAGTGTT
    GTGCAGCGAA GAATCATTTT CTTCCAAGAC GAGGGCTCGC TTACCAAAAG ATTGTGCGAG
    AAAGATCCTG TTGCGATTAC TGAAAAACCT GCTCAAGAGT GTTGTGCTTG TGGAACAGCC
    AAGTATAGAG TGACTTTCTA TGGAAACTGG TCGGAGAAGC TCCATCCCAA AGATTACCCA
    AGACGTGCTA ATCATTGGTC GGCTCTCATT GGCGCGTCTC ACACTAGAAG TTATGTGTTA
    TGGGAATATG GAGGATTTGC CAGTGAAGGA GTCAAGCAGG TGTCCGAGTA TGGATCACCC
    GTCAAAATGG AGGAGGAGAT CCGACAAAAG GGTGATGATG TTATGACAGT TATCAAAACC
    AAAGCCCAGT GGCCCGCCTG GCAGCCTCTT AATATTCGCG CCGCCCCATC AGCTGAGTTT
    TCGGTGGATC GAGTCCGACA CCTGATGTCC TTTTTGACCA TGCTGGGCCC CAGTCCTGAC
    TGGAACGTGG GTTTGTCTGG AGAAGATCTC TGCACCAGGG ATTGTGGCTG GGTCCAAAAA
    CTGGTGAAGG ATCTGGTCCC TTGGGATGCT GGGACTGATA GCGGTGTCTC TTATGAGTCT
    CCCAATAAAC CCAGCATTCC ACAGGAGCGC ATTCGACCCC TGACCAGCTT GGATCACCCA
    CAAAGCCCTT TTTATGACCC ATCAGGTGGT CCCATGACAC CACTCGGCCG AGTGGTGGTG
    GAGAGAATTG CAAGAAAGGG AGAGCAGTGC AATACCGTGC CCGACACGAT AGACGATATC
    GTCGCTGATA TTGCTCAGGA GGAGAAGGAG GAAGGAGATA ACACTCCAGA GACGTGCATT
    TACTCCAACT GGTCTCCGTG GTCGGCCTGC AGCTCCTCCA CCTGTGATAA GGGGCGGAGG
    ATGAGGCAGA GGATGCTAAA AGCGCAGCTG GACCCCAACC TGCCATGTCT GCACACCCAA
    GACTTTGAGC CCTGTATGGG GCCGGGCTGC AGCGAGGAGG AGGCCTCTAC TTGTATGATG
    TCTGAGTGGA TCAGCTGGTC ACCCTGCAGC GCTTCATGCG GGATGGGCAT GAGGTCCAGA
    GAGAGGTACG TCAAGCAGTT CCCAGAGGAC GGATCCAGCT GCACACTGCC AACAGAGGAG
    ACAGAGAAGT GTGTGGTCAA TGACGACTGC TCACCCAGTA GCTGTGTGGT GACGGAGTGG
    GGCGAGTGGG AGCCCTGTAG TGTAAGCTGT GGTTTGGGCA TGAGGAGGAG AGAACGCATG
    ATGAAGATGG ACGCCTCGGA CGGTTCGCCC TGTCGAGTGC AGATGGCAGA GGCAGAGAAG
    TGCATGATGC CCGAGTGCAA TGCGGTGGTG TGTATGCTGT CGCCATGGTC AGACTGGGGT
    GAGTGCAGTG TGTCCTGCGG TGTGGGCATG AGAACCCGTC AGCGCATGCT AAAGACACCC
    GTTAAGCCCG GCCAGTGTCC AGATGAGCTG GAGCAGGTGG AGAAGTGCAT GCTTCCCGAA
    TGCCCTACAG ACTGCATGCT GTCAGAGTGG TCAGCCTGGT CCGAGTGCAA CAAGTCCTGT
    GGGAAGGGGC ACATGATCCG CTCTCGCATG GTCAAGCTGG AGCCTCAGTT TGGAGGTCTG
    CCCTGTCCTG AGACGGTCCA GAGGAAGAAA TGCAAGATCC GCAAGTGCTC GAGAGGCTCC
    CGGGCCAGCG AGAAGAGGAA GAGACAGGAG GTTTCAGACA GGAGGAGAGC CAAGCAGAGC
    CGGGAGTCTG TGGCAGAGGA GAGCACAGTA GTCTGTCCAA TACGCCCTTG GTCCAGCTGG
    ACGGAGTGTA CTAAACCATG TGATGGGGGG ACTCAAGAGC GAGTGATGAC CATAAAGAAA
    AAGGGCAAAT CCTCACAGCA CAACCACTGC AAAAACAAGA AGGAGATCCG TGCGTGCAAC
    GTACACCCAT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.