piwil2 cDNA ORF clone, Danio rerio(zebrafish)

The following piwil2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the piwil2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa03533 NM_001080199.3
Latest version!
Danio rerio piwi-like RNA-mediated gene silencing 2 (piwil2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa10194 NM_001365624.1
Latest version!
Danio rerio piwi-like RNA-mediated gene silencing 2 (piwil2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa10194 XM_017356163.1
Latest version!
Danio rerio piwi-like RNA-mediated gene silencing 2 (piwil2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa10194 XM_021476307.1
Latest version!
Danio rerio piwi-like RNA-mediated gene silencing 2 (piwil2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa03533
    Clone ID Related Accession (Same CDS sequence) NM_001080199.3
    Accession Version NM_001080199.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Danio rerio(zebrafish)
    Product piwi-like protein 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF186090.2. On Aug 31, 2012 this sequence version replaced NM_001080199.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF186090.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA898400 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATCCAA AACGACCAAC CTTTCCCAGT CCCCCAGGGG TCATCCGGGC ACCATGGCAG 
    CAGTCGACAG AGGATCAGAG TCAACTTTTA GATCAGCCCT CATTGGGACG GGCCAGAGGT
    CTTATCATGC CCATAGATGA ACCTCTTCCT GGACGAGGTA GAGCTTTTAG TGTACTCGGA
    GAAATGCCGG TGAGATTTGG TAGAGGGATA ACTCAGTCTA TAGCTGCTGA GCCTCTGGTG
    GGCATGGCTA GAGGTGTGAG GTTACCTATG GAAGAGGGAG GTTTTGGGCG AGGACGAGGT
    TTTTTGCTGC CCACTCCTGA GCCCACTGTT GGGATAGGGA GAGGGGCTGC TATTGGACCA
    GTGCCTACAC TGGACATTCA AAAAGCAGAG GTGGAGGAAA AGATGCCTGA GCTGCAAGCG
    GAGGTGGCTC CAACTGTTGC CAAGGTTGGT TCCCCTGGGA CTGGATCGTC ACTGGTGTCC
    ATGTTCAGGG GTCTGGGAAT TGAGCCAGGA AAGACATGGG GTAGAGGAGC TGCACCTGTT
    GGACGGGGTG CAGCAGGCGA TATGGGGGCA GATTTGCAGC CAAAACCCAC CATCATTGGA
    GCATCTCTGA CTCCAGAAAG AGAAGAGGTT CGCTCAGAAG AATCGATCAG TTTCCTGGGA
    AGAGGCTTTA CTGGTTTTGG CAGAGCAGCA ATGCCTCATA TGACTGTTGG GCGTGGACCG
    ATTGGCCCAT TAAGTCCTTC ACCTTCTGTC GCTGCCCCTT TCTCTTTGAT TTCTGCTTCC
    TCATCCTCTG AAGATGCCCC GGTTGCTCCA GGAACACCTC CTAAAGTTGA AGTCAAAATT
    GAGACAGTGA AGGAGCCTCT GCAGAAGATA GGAACTAAAG GCAGTCCCAT ACCCATTGGA
    TCAAACTACA TCCCTATCTG CTGCAAAAAC GATGCAGTGT TTCAGTACCA TGTTACTTTT
    ACGCCGAACG TTGAATCACT TAGCATGCGC TTTGGAATGA TGAAGGAGCA TCGGCCCACT
    ACTGGAGAAG TAGTGGCTTT TGACGGATCT ATCCTCTACC TGCCAAAACG TCTGGAAGAG
    GTGGTTCATC TAAAAGCTGA AAGGAAAACC GACAATCAGG AGATCGACAT CAAGATCCAA
    TTGACGAAGA TTCTGCCTCC CAGCTCTGAC CTTTGTATCC CGTTTTACAA CGTGGTCTTA
    AGGAGAGTGA TGAAAATACT GGGGCTGAAG CTGGTGGGCA GAAACCATTA CGACCCCAAT
    GCGGTTGTGA TCCTGGGCAA ACACAGGCTT CAGGTGTGGC CTGGATACTC CACCAGTATT
    AAGCACACTG ACGGTGGCCT CTACCTGGTT GTGGATGTCT CGCATAAGGT GCTGCGAAAC
    GACTCTGTGC TGGATGTCAT GAATCTGATT TACCAAGGAA GCCGTGAGAG CTTTCAGGAT
    GAATGCACAA AGGAGTTTGT GGGTTCAATC GTCATCACAC GCTATAATAA CCGCACTTAC
    CGTATTGATG ACATCGAGTG GTCCAAGTCT CCCAAAGACA CTTTCACCTT GGCTGATGGA
    TCTGTGACCA CGTTTGTCGA TTACTACAGA AAGAACTATG GGATTACCAT AAAGGAACTG
    GACCAACCGC TGCTCATTCA TCGTCCAAAA GAGAGGTCCA GACCAGGAGG AAAGGTTATA
    ACAGGTGAAA TCCTTCTGTT GCCGGAGCTT TCCTTCATGA CTGGCATCCC TGAGAAAATG
    AGGAAAGACT TCAGGGCCAT GAAGGATCTG ACCATGCACA TCAATGTGGG AGCTCAGCAA
    CACACCCAGA GTCTCAAGCA GCTGCTGCAC AACATCAACA GCAACAATGA AGCTCTCTCA
    GAGCTGGGCC GCTGGGGCCT CAGCATCAGC CAGGAGATTC TAGTGACTCA AGGAAGAACT
    CTGCCCAGTG AGACCATCTG CTTGCATTCA GCCTCTTTTG TCACATCTCC GGCAGTAGAC
    TGGTCCAGAG AGTTGGTTCG AGACCCCTCC ATCAGCACTG TCCCTCTGAA TTGCTGGGCT
    GTTTTCTATC CCAGACGGGC AACCGATCAG GCTGAGGAGC TGGTGACCAC TTTTTCAAGG
    GTAGCTGGTC CTATGGGCAT GAGAGTGGAA CGACCCATCC GTGTAGAGTT GCGCGATGAT
    CGAACCGAGA CCTTCGTCAA GAGCATTCAC TCTCAACTCG CCAGCGAGCC TCGTGTACAG
    CTAGTCGTGT GCATCATGAC AGGAAACAGA GATGACCTGT ATAGTGCCAT CAAAAAGCTT
    TGCTGCATCC AGAGTCCAGT ACCGTCACAG GCAATCAACG TTCGGACCAT CTCACAGCCA
    CAGAAGCTCC GCAGTGTTGC TCAAAAGATC CTTTTGCAGA TTAACTGCAA GCTTGGAGGA
    GAGCTGTGGA CGGTGGACGT TCCTCTGAAA TACTTGATGG TGATTGGAGT GGATGTTCGC
    CATGATACCA GCAAGAAGAG CAGATCTGTC ATGGGCTTCG TGGCAAGTCT CAACAGCATG
    TTGACCAAAT GGTACTCCAG GGTGACCTTC CAAATGCCTA ATGAGGAGAT TATCAACGGC
    TTTAGAGTGT GTCTTCTCGC TGCCCTGCAG AAATACTATG AGGTGAACCA TGCCTTCCCT
    GAGAAGATTG TCATCTATCG CGACGGTGTG TCTGACGGAC AGCTGAAGAC CGTGGAGCAC
    TACGAGATCC CTCAGATTCT GAAATGCTTC GAGACCATCC CAAACTACGA GCCTAAGCTT
    GCATTCATCG TGGTGCAGAA AAGAATCAGC ACAACCCTGT ACTCCTACGG GTCAGACCAC
    TTCGGGACAC CCTCGCCAGG AACTGTGCTG GACCACACTG TGACAAACAG AGACTGGGTG
    GACTTCTACC TAATGGCACA TTCCATTCGA CAGGGTTGTG GCCTGCCCAC TCATTACATA
    ACAGTGTACA ACACCGCAAA CCTCACCCCT GACCACCTGC AGAGGTTGAC CTTCAAGATG
    TGTCACCTGT ACTGGAACTG GCCTGGAACA ATACGCGTTC CTGCACCGTG CAAATATGCC
    CACAAACTGG CTTTCCTGTC TGGCCAGTAC CTCCATTCAG AGCCTGCAAT CCAGCTGTCA
    GAGAAACTGT TTTTTCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001073668.2
    CDS280..3420
    Translation

    Target ORF information:

    RefSeq Version NM_001080199.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio piwi-like RNA-mediated gene silencing 2 (piwil2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001080199.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATCCAA AACGACCAAC CTTTCCCAGT CCCCCAGGGG TCATCCGGGC ACCATGGCAG 
    CAGTCGACAG AGGATCAGAG TCAACTTTTA GATCAGCCCT CATTGGGACG GGCCAGAGGT
    CTTATCATGC CCATAGATGA ACCTCTTCCT GGACGAGGTA GAGCTTTTAG TGTACTCGGA
    GAAATGCCGG TGAGATTTGG TAGAGGGATA ACTCAGTCTA TAGCTGCTGA GCCTCTGGTG
    GGCATGGCTA GAGGTGTGAG GTTACCTATG GAAGAGGGAG GTTTTGGGCG AGGACGAGGT
    TTTTTGCTGC CCACTCCTGA GCCCACTGTT GGGATAGGGA GAGGGGCTGC TATTGGACCA
    GTGCCTACAC TGGACATTCA AAAAGCAGAG GTGGAGGAAA AGATGCCTGA GCTGCAAGCG
    GAGGTGGCTC CAACTGTTGC CAAGGTTGGT TCCCCTGGGA CTGGATCGTC ACTGGTGTCC
    ATGTTCAGGG GTCTGGGAAT TGAGCCAGGA AAGACATGGG GTAGAGGAGC TGCACCTGTT
    GGACGGGGTG CAGCAGGCGA TATGGGGGCA GATTTGCAGC CAAAACCCAC CATCATTGGA
    GCATCTCTGA CTCCAGAAAG AGAAGAGGTT CGCTCAGAAG AATCGATCAG TTTCCTGGGA
    AGAGGCTTTA CTGGTTTTGG CAGAGCAGCA ATGCCTCATA TGACTGTTGG GCGTGGACCG
    ATTGGCCCAT TAAGTCCTTC ACCTTCTGTC GCTGCCCCTT TCTCTTTGAT TTCTGCTTCC
    TCATCCTCTG AAGATGCCCC GGTTGCTCCA GGAACACCTC CTAAAGTTGA AGTCAAAATT
    GAGACAGTGA AGGAGCCTCT GCAGAAGATA GGAACTAAAG GCAGTCCCAT ACCCATTGGA
    TCAAACTACA TCCCTATCTG CTGCAAAAAC GATGCAGTGT TTCAGTACCA TGTTACTTTT
    ACGCCGAACG TTGAATCACT TAGCATGCGC TTTGGAATGA TGAAGGAGCA TCGGCCCACT
    ACTGGAGAAG TAGTGGCTTT TGACGGATCT ATCCTCTACC TGCCAAAACG TCTGGAAGAG
    GTGGTTCATC TAAAAGCTGA AAGGAAAACC GACAATCAGG AGATCGACAT CAAGATCCAA
    TTGACGAAGA TTCTGCCTCC CAGCTCTGAC CTTTGTATCC CGTTTTACAA CGTGGTCTTA
    AGGAGAGTGA TGAAAATACT GGGGCTGAAG CTGGTGGGCA GAAACCATTA CGACCCCAAT
    GCGGTTGTGA TCCTGGGCAA ACACAGGCTT CAGGTGTGGC CTGGATACTC CACCAGTATT
    AAGCACACTG ACGGTGGCCT CTACCTGGTT GTGGATGTCT CGCATAAGGT GCTGCGAAAC
    GACTCTGTGC TGGATGTCAT GAATCTGATT TACCAAGGAA GCCGTGAGAG CTTTCAGGAT
    GAATGCACAA AGGAGTTTGT GGGTTCAATC GTCATCACAC GCTATAATAA CCGCACTTAC
    CGTATTGATG ACATCGAGTG GTCCAAGTCT CCCAAAGACA CTTTCACCTT GGCTGATGGA
    TCTGTGACCA CGTTTGTCGA TTACTACAGA AAGAACTATG GGATTACCAT AAAGGAACTG
    GACCAACCGC TGCTCATTCA TCGTCCAAAA GAGAGGTCCA GACCAGGAGG AAAGGTTATA
    ACAGGTGAAA TCCTTCTGTT GCCGGAGCTT TCCTTCATGA CTGGCATCCC TGAGAAAATG
    AGGAAAGACT TCAGGGCCAT GAAGGATCTG ACCATGCACA TCAATGTGGG AGCTCAGCAA
    CACACCCAGA GTCTCAAGCA GCTGCTGCAC AACATCAACA GCAACAATGA AGCTCTCTCA
    GAGCTGGGCC GCTGGGGCCT CAGCATCAGC CAGGAGATTC TAGTGACTCA AGGAAGAACT
    CTGCCCAGTG AGACCATCTG CTTGCATTCA GCCTCTTTTG TCACATCTCC GGCAGTAGAC
    TGGTCCAGAG AGTTGGTTCG AGACCCCTCC ATCAGCACTG TCCCTCTGAA TTGCTGGGCT
    GTTTTCTATC CCAGACGGGC AACCGATCAG GCTGAGGAGC TGGTGACCAC TTTTTCAAGG
    GTAGCTGGTC CTATGGGCAT GAGAGTGGAA CGACCCATCC GTGTAGAGTT GCGCGATGAT
    CGAACCGAGA CCTTCGTCAA GAGCATTCAC TCTCAACTCG CCAGCGAGCC TCGTGTACAG
    CTAGTCGTGT GCATCATGAC AGGAAACAGA GATGACCTGT ATAGTGCCAT CAAAAAGCTT
    TGCTGCATCC AGAGTCCAGT ACCGTCACAG GCAATCAACG TTCGGACCAT CTCACAGCCA
    CAGAAGCTCC GCAGTGTTGC TCAAAAGATC CTTTTGCAGA TTAACTGCAA GCTTGGAGGA
    GAGCTGTGGA CGGTGGACGT TCCTCTGAAA TACTTGATGG TGATTGGAGT GGATGTTCGC
    CATGATACCA GCAAGAAGAG CAGATCTGTC ATGGGCTTCG TGGCAAGTCT CAACAGCATG
    TTGACCAAAT GGTACTCCAG GGTGACCTTC CAAATGCCTA ATGAGGAGAT TATCAACGGC
    TTTAGAGTGT GTCTTCTCGC TGCCCTGCAG AAATACTATG AGGTGAACCA TGCCTTCCCT
    GAGAAGATTG TCATCTATCG CGACGGTGTG TCTGACGGAC AGCTGAAGAC CGTGGAGCAC
    TACGAGATCC CTCAGATTCT GAAATGCTTC GAGACCATCC CAAACTACGA GCCTAAGCTT
    GCATTCATCG TGGTGCAGAA AAGAATCAGC ACAACCCTGT ACTCCTACGG GTCAGACCAC
    TTCGGGACAC CCTCGCCAGG AACTGTGCTG GACCACACTG TGACAAACAG AGACTGGGTG
    GACTTCTACC TAATGGCACA TTCCATTCGA CAGGGTTGTG GCCTGCCCAC TCATTACATA
    ACAGTGTACA ACACCGCAAA CCTCACCCCT GACCACCTGC AGAGGTTGAC CTTCAAGATG
    TGTCACCTGT ACTGGAACTG GCCTGGAACA ATACGCGTTC CTGCACCGTG CAAATATGCC
    CACAAACTGG CTTTCCTGTC TGGCCAGTAC CTCCATTCAG AGCCTGCAAT CCAGCTGTCA
    GAGAAACTGT TTTTTCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10194
    Clone ID Related Accession (Same CDS sequence) NM_001365624.1 , XM_017356163.1 , XM_021476307.1
    Accession Version NM_001365624.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-14
    Organism Danio rerio(zebrafish)
    Product piwi-like protein 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR450719.8. On or before Sep 7, 2018 this sequence version replaced NM_001080199.3, XM_021476307.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01030434.1, EU817487.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA898400 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATCCAA AACGACCAAC CTTTCCCAGT CCCCCAGGGG TCATCCGGGC ACCATGGCAG 
    CAGTCGACAG AGGATCAGAG TCAACTTTTA GATCAGCCCT CATTGGGACG GGCCAGAGGT
    CTTATCATGC CCATAGATGA ACCTCTTCCT GGACGAGGTA GAGCTTTTAG TGTACCCGGA
    GAAATGCCGG TGAGATTTGG TAGAGGGATA ACTCAGTCTA TAGCTGCTGA GCCTCTGGTG
    GGCATGGCTA GAGGTGTGAG GTTACCTATG GAAGAGGGAG GTTTTGGGCG AGGACGAGGT
    TTTTTGCTGC CCACTCCTGA GCCCACTGTT GGGATAGGGA GAGGGGCTGC TATTGGACCA
    GTGCCTACAC TGGACATTCA AAAAGCAGAG GTGGAGGAAA AGATGCCTGA GCTGCAAGCG
    GAGGTGGCTC CAACTGTTGC CAAGGTTGGT TCCCCTGGGA CTGGATCGTC ACTGGTGTCC
    ATGTTCAGGG GTCTGGGAAT TGAGCCAGGA AAGACATGGG GTAGAGGAGC TGCACCTGTT
    GGACGGGGTG CAGCAGGCGA TATGGGGGCA GATTTGCAGC CAAAACCCAC CATCATTGGA
    GCATCTCTGA CTCCAGAAAG AGAAGAGGTT CGCTCAGAAG AATCGATCAG TTTCCTGGGA
    AGAGGCTTTA CTGGTTTTGG CAGAGCAGCA ATGCCTCATA TGACTGTTGG GCGTGGACCG
    ATTGGACCAT TAAGTCCTTC ACCTTCTGTC GCTGCCCCTT TCTCTTTGAT TTCTGCTTCC
    TCTGCCTCTG AAGATGCCCC GGTTGCTCCA GGAACACCTC CTAAAGTTGA AGTCAAAATT
    GAGACAGTGA AGGAGCCTCT GCAGAAGATA GGAACTAAAG GCAGTCCCAT ACCCATTGGA
    TCAAACTACA TCCCTATCTG CTGCAAAAAC GATGCAGTGT TTCAGTACCA TGTTACTTTT
    ACGCCGAACG TTGAATCACT TAGCATGCGC TTCGGAATGA TGAAGGAGCA TCGGCCCACT
    ACTGGAGAAG TAGTGGCTTT TGACGGATCT ATCCTCTACC TGCCAAAACG TCTGGAAGAG
    GTGGTTCATC TAAAAGCTGA AAGGAAAACC GACAATCAGG AGATCGACAT CAAGATCCAA
    TTGACGAAGA TTCTGCCTCC CAGCTCTGAC CTTTGTATCC CGTTTTACAA CGTGGTCTTA
    AGGAGAGTGA TGAAAATACT GGGGCTGAAG CTGGTGGGCA GAAACCATTA CGACCCCAAT
    GCGGTTGTGA TCCTGGGCAA ACACAGGCTT CAGGTGTGGC CTGGATACTC CACCAGTATT
    AAGCACACTG ACGGTGGCCT CTACCTGGTT GTGGATGTCT CGCATAAGGT GCTGCGAAAC
    GACTCTGTGC TGGATGTCAT GAATCTGATT TACCAAGGAA GCCGTGAGAG CTTTCAGGAT
    GAATGCACAA AGGAGTTTGT GGGTTCAATC GTCATCACAC GCTATAATAA CCGCACTTAC
    CGTATTGATG ACATCGAGTG GTCCAAGTCT CCCAAAGACA CTTTCACCTT GGCTGATGGA
    TCTGTGACCA CGTTTGTCGA TTACTACAGA AAGAACTATG GGATTACCAT AAAGGAACTG
    GACCAACCGC TGCTCATTCA TCGTCCAAAA GAGAGGTCCA GACCAGGAGG AAAGGTTATA
    ACAGGTGAAA TCCTTTTGTT GCCGGAGCTT TCCTTCATGA CTGGCATCCC TGAGAAAATG
    AGGAAAGACT TCAGGGCCAT GAAGGATCTG ACCATGCACA TCAATGTGGG AGCTCAGCAA
    CACACCCAGA GTCTCAAGCA GCTGCTGCAC AACATCAACA GCAACAATGA AGCTCTCTCA
    GAGCTGGGCC GCTGGGGCCT CAGCATCAGC CAGGAGATTC TAGTGACTCA AGGAAGAACT
    CTGCCCAGTG AGACCATCTG CTTGCATTCA GCCTCTTTTG TCACATCTCC GGCAGTAGAC
    TGGTCCAGAG AGTTGGTTCG AGACCCCTCC ATCAGCACTG TCCCTCTGAA TTGCTGGGCT
    GTTTTCTATC CCAGACGGGC AACCGATCAG GCTGAGGAGC TGGTGACCAC TTTTTCAAGG
    GTAGCTGGTC CTATGGGCAT GAGAGTGGAA CGACCCATCC GTGTAGAGTT GCGCGATGAT
    CGAACCGAGA CCTTCGTCAA GAGCATTCAC TCTCAACTCA CCAGCGAGCC TCGTGTACAG
    CTAGTCGTGT GCATCATGAC AGGAAACAGA GATGACCTGT ATAGTGCCAT CAAAAAGCTT
    TGCTGCATCC AGAGTCCAGT ACCATCACAG GCAATCAACG TTCGGACCAT CTCACAGCCG
    CAGAAGCTCC GCAGTGTTGC TCAAAAGATC CTTTTGCAGA TTAACTGCAA GCTTGGAGGA
    GAGCTGTGGA CGGTGAACGT TCCTCTGAAA TACTTGATGG TGATTGGAGT GGATGTTCAC
    CATGATACCA GCAAGAAGAG CAGATCTGTC ATGGGCTTCG TGGCAAGTCT CAACAGCATG
    TTGACCAAAT GGTACTCCAG GGTGACCTTC CAAATGCCTA ATGAGGAGAT TATCAACGGC
    TTTAGAGTGT GTCTTCTCGC TGCCCTGCAG AAATACTATG AGGTGAACCA TGCCTTCCCT
    GAGAAGATTG TCATCTATCG CGACGGTGTG TCTGACGGAC AGCTGAAGAC CGTGGAGCAC
    TACGAGATCC CTCAGATTCT GAAATGCTTC GAGACCATCC CAAACTACGA GCCTAAGCTT
    GCATTCATCG TGGTGCAGAA AAGAATCAGC ACAACCCTGT ACTCCTACGG GTCAGACCAC
    TTCGGGACAC CCTCGCCAGG AACTGTGCTG GACCACACTG TGACAAACAG AGACTGGGTG
    GACTTCTACC TAATGGCACA TTCCATTCGA CAGGGTTGTG GCCTGCCCAC TCATTACATA
    ACAGTGTACA ACACCGCAAA CCTCACCCCT GACCACCTGC AGAGGTTGAC CTTCAAGATG
    TGTCACCTGT ACTGGAACTG GCCTGGAACA ATACGCGTTC CTGCACCGTG CAAATATGCC
    CACAAACTGG CTTTCCTGTC TGGCCAGTAC CTCCATTCAG AGCCTGCAAT CCAGCTGTCA
    GAGAAACTGT TTTTTCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352553.1
    CDS108..3248
    Translation

    Target ORF information:

    RefSeq Version NM_001365624.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio piwi-like RNA-mediated gene silencing 2 (piwil2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATCCAA AACGACCAAC CTTTCCCAGT CCCCCAGGGG TCATCCGGGC ACCATGGCAG 
    CAGTCGACAG AGGATCAGAG TCAACTTTTA GATCAGCCCT CATTGGGACG GGCCAGAGGT
    CTTATCATGC CCATAGATGA ACCTCTTCCT GGACGAGGTA GAGCTTTTAG TGTACCCGGA
    GAAATGCCGG TGAGATTTGG TAGAGGGATA ACTCAGTCTA TAGCTGCTGA GCCTCTGGTG
    GGCATGGCTA GAGGTGTGAG GTTACCTATG GAAGAGGGAG GTTTTGGGCG AGGACGAGGT
    TTTTTGCTGC CCACTCCTGA GCCCACTGTT GGGATAGGGA GAGGGGCTGC TATTGGACCA
    GTGCCTACAC TGGACATTCA AAAAGCAGAG GTGGAGGAAA AGATGCCTGA GCTGCAAGCG
    GAGGTGGCTC CAACTGTTGC CAAGGTTGGT TCCCCTGGGA CTGGATCGTC ACTGGTGTCC
    ATGTTCAGGG GTCTGGGAAT TGAGCCAGGA AAGACATGGG GTAGAGGAGC TGCACCTGTT
    GGACGGGGTG CAGCAGGCGA TATGGGGGCA GATTTGCAGC CAAAACCCAC CATCATTGGA
    GCATCTCTGA CTCCAGAAAG AGAAGAGGTT CGCTCAGAAG AATCGATCAG TTTCCTGGGA
    AGAGGCTTTA CTGGTTTTGG CAGAGCAGCA ATGCCTCATA TGACTGTTGG GCGTGGACCG
    ATTGGACCAT TAAGTCCTTC ACCTTCTGTC GCTGCCCCTT TCTCTTTGAT TTCTGCTTCC
    TCTGCCTCTG AAGATGCCCC GGTTGCTCCA GGAACACCTC CTAAAGTTGA AGTCAAAATT
    GAGACAGTGA AGGAGCCTCT GCAGAAGATA GGAACTAAAG GCAGTCCCAT ACCCATTGGA
    TCAAACTACA TCCCTATCTG CTGCAAAAAC GATGCAGTGT TTCAGTACCA TGTTACTTTT
    ACGCCGAACG TTGAATCACT TAGCATGCGC TTCGGAATGA TGAAGGAGCA TCGGCCCACT
    ACTGGAGAAG TAGTGGCTTT TGACGGATCT ATCCTCTACC TGCCAAAACG TCTGGAAGAG
    GTGGTTCATC TAAAAGCTGA AAGGAAAACC GACAATCAGG AGATCGACAT CAAGATCCAA
    TTGACGAAGA TTCTGCCTCC CAGCTCTGAC CTTTGTATCC CGTTTTACAA CGTGGTCTTA
    AGGAGAGTGA TGAAAATACT GGGGCTGAAG CTGGTGGGCA GAAACCATTA CGACCCCAAT
    GCGGTTGTGA TCCTGGGCAA ACACAGGCTT CAGGTGTGGC CTGGATACTC CACCAGTATT
    AAGCACACTG ACGGTGGCCT CTACCTGGTT GTGGATGTCT CGCATAAGGT GCTGCGAAAC
    GACTCTGTGC TGGATGTCAT GAATCTGATT TACCAAGGAA GCCGTGAGAG CTTTCAGGAT
    GAATGCACAA AGGAGTTTGT GGGTTCAATC GTCATCACAC GCTATAATAA CCGCACTTAC
    CGTATTGATG ACATCGAGTG GTCCAAGTCT CCCAAAGACA CTTTCACCTT GGCTGATGGA
    TCTGTGACCA CGTTTGTCGA TTACTACAGA AAGAACTATG GGATTACCAT AAAGGAACTG
    GACCAACCGC TGCTCATTCA TCGTCCAAAA GAGAGGTCCA GACCAGGAGG AAAGGTTATA
    ACAGGTGAAA TCCTTTTGTT GCCGGAGCTT TCCTTCATGA CTGGCATCCC TGAGAAAATG
    AGGAAAGACT TCAGGGCCAT GAAGGATCTG ACCATGCACA TCAATGTGGG AGCTCAGCAA
    CACACCCAGA GTCTCAAGCA GCTGCTGCAC AACATCAACA GCAACAATGA AGCTCTCTCA
    GAGCTGGGCC GCTGGGGCCT CAGCATCAGC CAGGAGATTC TAGTGACTCA AGGAAGAACT
    CTGCCCAGTG AGACCATCTG CTTGCATTCA GCCTCTTTTG TCACATCTCC GGCAGTAGAC
    TGGTCCAGAG AGTTGGTTCG AGACCCCTCC ATCAGCACTG TCCCTCTGAA TTGCTGGGCT
    GTTTTCTATC CCAGACGGGC AACCGATCAG GCTGAGGAGC TGGTGACCAC TTTTTCAAGG
    GTAGCTGGTC CTATGGGCAT GAGAGTGGAA CGACCCATCC GTGTAGAGTT GCGCGATGAT
    CGAACCGAGA CCTTCGTCAA GAGCATTCAC TCTCAACTCA CCAGCGAGCC TCGTGTACAG
    CTAGTCGTGT GCATCATGAC AGGAAACAGA GATGACCTGT ATAGTGCCAT CAAAAAGCTT
    TGCTGCATCC AGAGTCCAGT ACCATCACAG GCAATCAACG TTCGGACCAT CTCACAGCCG
    CAGAAGCTCC GCAGTGTTGC TCAAAAGATC CTTTTGCAGA TTAACTGCAA GCTTGGAGGA
    GAGCTGTGGA CGGTGAACGT TCCTCTGAAA TACTTGATGG TGATTGGAGT GGATGTTCAC
    CATGATACCA GCAAGAAGAG CAGATCTGTC ATGGGCTTCG TGGCAAGTCT CAACAGCATG
    TTGACCAAAT GGTACTCCAG GGTGACCTTC CAAATGCCTA ATGAGGAGAT TATCAACGGC
    TTTAGAGTGT GTCTTCTCGC TGCCCTGCAG AAATACTATG AGGTGAACCA TGCCTTCCCT
    GAGAAGATTG TCATCTATCG CGACGGTGTG TCTGACGGAC AGCTGAAGAC CGTGGAGCAC
    TACGAGATCC CTCAGATTCT GAAATGCTTC GAGACCATCC CAAACTACGA GCCTAAGCTT
    GCATTCATCG TGGTGCAGAA AAGAATCAGC ACAACCCTGT ACTCCTACGG GTCAGACCAC
    TTCGGGACAC CCTCGCCAGG AACTGTGCTG GACCACACTG TGACAAACAG AGACTGGGTG
    GACTTCTACC TAATGGCACA TTCCATTCGA CAGGGTTGTG GCCTGCCCAC TCATTACATA
    ACAGTGTACA ACACCGCAAA CCTCACCCCT GACCACCTGC AGAGGTTGAC CTTCAAGATG
    TGTCACCTGT ACTGGAACTG GCCTGGAACA ATACGCGTTC CTGCACCGTG CAAATATGCC
    CACAAACTGG CTTTCCTGTC TGGCCAGTAC CTCCATTCAG AGCCTGCAAT CCAGCTGTCA
    GAGAAACTGT TTTTTCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10194
    Clone ID Related Accession (Same CDS sequence) NM_001365624.1 , XM_017356163.1 , XM_021476307.1
    Accession Version XM_017356163.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product piwi-like protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATCCAA AACGACCAAC CTTTCCCAGT CCCCCAGGGG TCATCCGGGC ACCATGGCAG 
    CAGTCGACAG AGGATCAGAG TCAACTTTTA GATCAGCCCT CATTGGGACG GGCCAGAGGT
    CTTATCATGC CCATAGATGA ACCTCTTCCT GGACGAGGTA GAGCTTTTAG TGTACCCGGA
    GAAATGCCGG TGAGATTTGG TAGAGGGATA ACTCAGTCTA TAGCTGCTGA GCCTCTGGTG
    GGCATGGCTA GAGGTGTGAG GTTACCTATG GAAGAGGGAG GTTTTGGGCG AGGACGAGGT
    TTTTTGCTGC CCACTCCTGA GCCCACTGTT GGGATAGGGA GAGGGGCTGC TATTGGACCA
    GTGCCTACAC TGGACATTCA AAAAGCAGAG GTGGAGGAAA AGATGCCTGA GCTGCAAGCG
    GAGGTGGCTC CAACTGTTGC CAAGGTTGGT TCCCCTGGGA CTGGATCGTC ACTGGTGTCC
    ATGTTCAGGG GTCTGGGAAT TGAGCCAGGA AAGACATGGG GTAGAGGAGC TGCACCTGTT
    GGACGGGGTG CAGCAGGCGA TATGGGGGCA GATTTGCAGC CAAAACCCAC CATCATTGGA
    GCATCTCTGA CTCCAGAAAG AGAAGAGGTT CGCTCAGAAG AATCGATCAG TTTCCTGGGA
    AGAGGCTTTA CTGGTTTTGG CAGAGCAGCA ATGCCTCATA TGACTGTTGG GCGTGGACCG
    ATTGGACCAT TAAGTCCTTC ACCTTCTGTC GCTGCCCCTT TCTCTTTGAT TTCTGCTTCC
    TCTGCCTCTG AAGATGCCCC GGTTGCTCCA GGAACACCTC CTAAAGTTGA AGTCAAAATT
    GAGACAGTGA AGGAGCCTCT GCAGAAGATA GGAACTAAAG GCAGTCCCAT ACCCATTGGA
    TCAAACTACA TCCCTATCTG CTGCAAAAAC GATGCAGTGT TTCAGTACCA TGTTACTTTT
    ACGCCGAACG TTGAATCACT TAGCATGCGC TTCGGAATGA TGAAGGAGCA TCGGCCCACT
    ACTGGAGAAG TAGTGGCTTT TGACGGATCT ATCCTCTACC TGCCAAAACG TCTGGAAGAG
    GTGGTTCATC TAAAAGCTGA AAGGAAAACC GACAATCAGG AGATCGACAT CAAGATCCAA
    TTGACGAAGA TTCTGCCTCC CAGCTCTGAC CTTTGTATCC CGTTTTACAA CGTGGTCTTA
    AGGAGAGTGA TGAAAATACT GGGGCTGAAG CTGGTGGGCA GAAACCATTA CGACCCCAAT
    GCGGTTGTGA TCCTGGGCAA ACACAGGCTT CAGGTGTGGC CTGGATACTC CACCAGTATT
    AAGCACACTG ACGGTGGCCT CTACCTGGTT GTGGATGTCT CGCATAAGGT GCTGCGAAAC
    GACTCTGTGC TGGATGTCAT GAATCTGATT TACCAAGGAA GCCGTGAGAG CTTTCAGGAT
    GAATGCACAA AGGAGTTTGT GGGTTCAATC GTCATCACAC GCTATAATAA CCGCACTTAC
    CGTATTGATG ACATCGAGTG GTCCAAGTCT CCCAAAGACA CTTTCACCTT GGCTGATGGA
    TCTGTGACCA CGTTTGTCGA TTACTACAGA AAGAACTATG GGATTACCAT AAAGGAACTG
    GACCAACCGC TGCTCATTCA TCGTCCAAAA GAGAGGTCCA GACCAGGAGG AAAGGTTATA
    ACAGGTGAAA TCCTTTTGTT GCCGGAGCTT TCCTTCATGA CTGGCATCCC TGAGAAAATG
    AGGAAAGACT TCAGGGCCAT GAAGGATCTG ACCATGCACA TCAATGTGGG AGCTCAGCAA
    CACACCCAGA GTCTCAAGCA GCTGCTGCAC AACATCAACA GCAACAATGA AGCTCTCTCA
    GAGCTGGGCC GCTGGGGCCT CAGCATCAGC CAGGAGATTC TAGTGACTCA AGGAAGAACT
    CTGCCCAGTG AGACCATCTG CTTGCATTCA GCCTCTTTTG TCACATCTCC GGCAGTAGAC
    TGGTCCAGAG AGTTGGTTCG AGACCCCTCC ATCAGCACTG TCCCTCTGAA TTGCTGGGCT
    GTTTTCTATC CCAGACGGGC AACCGATCAG GCTGAGGAGC TGGTGACCAC TTTTTCAAGG
    GTAGCTGGTC CTATGGGCAT GAGAGTGGAA CGACCCATCC GTGTAGAGTT GCGCGATGAT
    CGAACCGAGA CCTTCGTCAA GAGCATTCAC TCTCAACTCA CCAGCGAGCC TCGTGTACAG
    CTAGTCGTGT GCATCATGAC AGGAAACAGA GATGACCTGT ATAGTGCCAT CAAAAAGCTT
    TGCTGCATCC AGAGTCCAGT ACCATCACAG GCAATCAACG TTCGGACCAT CTCACAGCCG
    CAGAAGCTCC GCAGTGTTGC TCAAAAGATC CTTTTGCAGA TTAACTGCAA GCTTGGAGGA
    GAGCTGTGGA CGGTGAACGT TCCTCTGAAA TACTTGATGG TGATTGGAGT GGATGTTCAC
    CATGATACCA GCAAGAAGAG CAGATCTGTC ATGGGCTTCG TGGCAAGTCT CAACAGCATG
    TTGACCAAAT GGTACTCCAG GGTGACCTTC CAAATGCCTA ATGAGGAGAT TATCAACGGC
    TTTAGAGTGT GTCTTCTCGC TGCCCTGCAG AAATACTATG AGGTGAACCA TGCCTTCCCT
    GAGAAGATTG TCATCTATCG CGACGGTGTG TCTGACGGAC AGCTGAAGAC CGTGGAGCAC
    TACGAGATCC CTCAGATTCT GAAATGCTTC GAGACCATCC CAAACTACGA GCCTAAGCTT
    GCATTCATCG TGGTGCAGAA AAGAATCAGC ACAACCCTGT ACTCCTACGG GTCAGACCAC
    TTCGGGACAC CCTCGCCAGG AACTGTGCTG GACCACACTG TGACAAACAG AGACTGGGTG
    GACTTCTACC TAATGGCACA TTCCATTCGA CAGGGTTGTG GCCTGCCCAC TCATTACATA
    ACAGTGTACA ACACCGCAAA CCTCACCCCT GACCACCTGC AGAGGTTGAC CTTCAAGATG
    TGTCACCTGT ACTGGAACTG GCCTGGAACA ATACGCGTTC CTGCACCGTG CAAATATGCC
    CACAAACTGG CTTTCCTGTC TGGCCAGTAC CTCCATTCAG AGCCTGCAAT CCAGCTGTCA
    GAGAAACTGT TTTTTCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211652.1
    CDS160..3300
    Translation

    Target ORF information:

    RefSeq Version XM_017356163.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio piwi-like RNA-mediated gene silencing 2 (piwil2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356163.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATCCAA AACGACCAAC CTTTCCCAGT CCCCCAGGGG TCATCCGGGC ACCATGGCAG 
    CAGTCGACAG AGGATCAGAG TCAACTTTTA GATCAGCCCT CATTGGGACG GGCCAGAGGT
    CTTATCATGC CCATAGATGA ACCTCTTCCT GGACGAGGTA GAGCTTTTAG TGTACCCGGA
    GAAATGCCGG TGAGATTTGG TAGAGGGATA ACTCAGTCTA TAGCTGCTGA GCCTCTGGTG
    GGCATGGCTA GAGGTGTGAG GTTACCTATG GAAGAGGGAG GTTTTGGGCG AGGACGAGGT
    TTTTTGCTGC CCACTCCTGA GCCCACTGTT GGGATAGGGA GAGGGGCTGC TATTGGACCA
    GTGCCTACAC TGGACATTCA AAAAGCAGAG GTGGAGGAAA AGATGCCTGA GCTGCAAGCG
    GAGGTGGCTC CAACTGTTGC CAAGGTTGGT TCCCCTGGGA CTGGATCGTC ACTGGTGTCC
    ATGTTCAGGG GTCTGGGAAT TGAGCCAGGA AAGACATGGG GTAGAGGAGC TGCACCTGTT
    GGACGGGGTG CAGCAGGCGA TATGGGGGCA GATTTGCAGC CAAAACCCAC CATCATTGGA
    GCATCTCTGA CTCCAGAAAG AGAAGAGGTT CGCTCAGAAG AATCGATCAG TTTCCTGGGA
    AGAGGCTTTA CTGGTTTTGG CAGAGCAGCA ATGCCTCATA TGACTGTTGG GCGTGGACCG
    ATTGGACCAT TAAGTCCTTC ACCTTCTGTC GCTGCCCCTT TCTCTTTGAT TTCTGCTTCC
    TCTGCCTCTG AAGATGCCCC GGTTGCTCCA GGAACACCTC CTAAAGTTGA AGTCAAAATT
    GAGACAGTGA AGGAGCCTCT GCAGAAGATA GGAACTAAAG GCAGTCCCAT ACCCATTGGA
    TCAAACTACA TCCCTATCTG CTGCAAAAAC GATGCAGTGT TTCAGTACCA TGTTACTTTT
    ACGCCGAACG TTGAATCACT TAGCATGCGC TTCGGAATGA TGAAGGAGCA TCGGCCCACT
    ACTGGAGAAG TAGTGGCTTT TGACGGATCT ATCCTCTACC TGCCAAAACG TCTGGAAGAG
    GTGGTTCATC TAAAAGCTGA AAGGAAAACC GACAATCAGG AGATCGACAT CAAGATCCAA
    TTGACGAAGA TTCTGCCTCC CAGCTCTGAC CTTTGTATCC CGTTTTACAA CGTGGTCTTA
    AGGAGAGTGA TGAAAATACT GGGGCTGAAG CTGGTGGGCA GAAACCATTA CGACCCCAAT
    GCGGTTGTGA TCCTGGGCAA ACACAGGCTT CAGGTGTGGC CTGGATACTC CACCAGTATT
    AAGCACACTG ACGGTGGCCT CTACCTGGTT GTGGATGTCT CGCATAAGGT GCTGCGAAAC
    GACTCTGTGC TGGATGTCAT GAATCTGATT TACCAAGGAA GCCGTGAGAG CTTTCAGGAT
    GAATGCACAA AGGAGTTTGT GGGTTCAATC GTCATCACAC GCTATAATAA CCGCACTTAC
    CGTATTGATG ACATCGAGTG GTCCAAGTCT CCCAAAGACA CTTTCACCTT GGCTGATGGA
    TCTGTGACCA CGTTTGTCGA TTACTACAGA AAGAACTATG GGATTACCAT AAAGGAACTG
    GACCAACCGC TGCTCATTCA TCGTCCAAAA GAGAGGTCCA GACCAGGAGG AAAGGTTATA
    ACAGGTGAAA TCCTTTTGTT GCCGGAGCTT TCCTTCATGA CTGGCATCCC TGAGAAAATG
    AGGAAAGACT TCAGGGCCAT GAAGGATCTG ACCATGCACA TCAATGTGGG AGCTCAGCAA
    CACACCCAGA GTCTCAAGCA GCTGCTGCAC AACATCAACA GCAACAATGA AGCTCTCTCA
    GAGCTGGGCC GCTGGGGCCT CAGCATCAGC CAGGAGATTC TAGTGACTCA AGGAAGAACT
    CTGCCCAGTG AGACCATCTG CTTGCATTCA GCCTCTTTTG TCACATCTCC GGCAGTAGAC
    TGGTCCAGAG AGTTGGTTCG AGACCCCTCC ATCAGCACTG TCCCTCTGAA TTGCTGGGCT
    GTTTTCTATC CCAGACGGGC AACCGATCAG GCTGAGGAGC TGGTGACCAC TTTTTCAAGG
    GTAGCTGGTC CTATGGGCAT GAGAGTGGAA CGACCCATCC GTGTAGAGTT GCGCGATGAT
    CGAACCGAGA CCTTCGTCAA GAGCATTCAC TCTCAACTCA CCAGCGAGCC TCGTGTACAG
    CTAGTCGTGT GCATCATGAC AGGAAACAGA GATGACCTGT ATAGTGCCAT CAAAAAGCTT
    TGCTGCATCC AGAGTCCAGT ACCATCACAG GCAATCAACG TTCGGACCAT CTCACAGCCG
    CAGAAGCTCC GCAGTGTTGC TCAAAAGATC CTTTTGCAGA TTAACTGCAA GCTTGGAGGA
    GAGCTGTGGA CGGTGAACGT TCCTCTGAAA TACTTGATGG TGATTGGAGT GGATGTTCAC
    CATGATACCA GCAAGAAGAG CAGATCTGTC ATGGGCTTCG TGGCAAGTCT CAACAGCATG
    TTGACCAAAT GGTACTCCAG GGTGACCTTC CAAATGCCTA ATGAGGAGAT TATCAACGGC
    TTTAGAGTGT GTCTTCTCGC TGCCCTGCAG AAATACTATG AGGTGAACCA TGCCTTCCCT
    GAGAAGATTG TCATCTATCG CGACGGTGTG TCTGACGGAC AGCTGAAGAC CGTGGAGCAC
    TACGAGATCC CTCAGATTCT GAAATGCTTC GAGACCATCC CAAACTACGA GCCTAAGCTT
    GCATTCATCG TGGTGCAGAA AAGAATCAGC ACAACCCTGT ACTCCTACGG GTCAGACCAC
    TTCGGGACAC CCTCGCCAGG AACTGTGCTG GACCACACTG TGACAAACAG AGACTGGGTG
    GACTTCTACC TAATGGCACA TTCCATTCGA CAGGGTTGTG GCCTGCCCAC TCATTACATA
    ACAGTGTACA ACACCGCAAA CCTCACCCCT GACCACCTGC AGAGGTTGAC CTTCAAGATG
    TGTCACCTGT ACTGGAACTG GCCTGGAACA ATACGCGTTC CTGCACCGTG CAAATATGCC
    CACAAACTGG CTTTCCTGTC TGGCCAGTAC CTCCATTCAG AGCCTGCAAT CCAGCTGTCA
    GAGAAACTGT TTTTTCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10194
    Clone ID Related Accession (Same CDS sequence) NM_001365624.1 , XM_017356163.1 , XM_021476307.1
    Accession Version XM_021476307.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3141bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product piwi-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATCCAA AACGACCAAC CTTTCCCAGT CCCCCAGGGG TCATCCGGGC ACCATGGCAG 
    CAGTCGACAG AGGATCAGAG TCAACTTTTA GATCAGCCCT CATTGGGACG GGCCAGAGGT
    CTTATCATGC CCATAGATGA ACCTCTTCCT GGACGAGGTA GAGCTTTTAG TGTACCCGGA
    GAAATGCCGG TGAGATTTGG TAGAGGGATA ACTCAGTCTA TAGCTGCTGA GCCTCTGGTG
    GGCATGGCTA GAGGTGTGAG GTTACCTATG GAAGAGGGAG GTTTTGGGCG AGGACGAGGT
    TTTTTGCTGC CCACTCCTGA GCCCACTGTT GGGATAGGGA GAGGGGCTGC TATTGGACCA
    GTGCCTACAC TGGACATTCA AAAAGCAGAG GTGGAGGAAA AGATGCCTGA GCTGCAAGCG
    GAGGTGGCTC CAACTGTTGC CAAGGTTGGT TCCCCTGGGA CTGGATCGTC ACTGGTGTCC
    ATGTTCAGGG GTCTGGGAAT TGAGCCAGGA AAGACATGGG GTAGAGGAGC TGCACCTGTT
    GGACGGGGTG CAGCAGGCGA TATGGGGGCA GATTTGCAGC CAAAACCCAC CATCATTGGA
    GCATCTCTGA CTCCAGAAAG AGAAGAGGTT CGCTCAGAAG AATCGATCAG TTTCCTGGGA
    AGAGGCTTTA CTGGTTTTGG CAGAGCAGCA ATGCCTCATA TGACTGTTGG GCGTGGACCG
    ATTGGACCAT TAAGTCCTTC ACCTTCTGTC GCTGCCCCTT TCTCTTTGAT TTCTGCTTCC
    TCTGCCTCTG AAGATGCCCC GGTTGCTCCA GGAACACCTC CTAAAGTTGA AGTCAAAATT
    GAGACAGTGA AGGAGCCTCT GCAGAAGATA GGAACTAAAG GCAGTCCCAT ACCCATTGGA
    TCAAACTACA TCCCTATCTG CTGCAAAAAC GATGCAGTGT TTCAGTACCA TGTTACTTTT
    ACGCCGAACG TTGAATCACT TAGCATGCGC TTCGGAATGA TGAAGGAGCA TCGGCCCACT
    ACTGGAGAAG TAGTGGCTTT TGACGGATCT ATCCTCTACC TGCCAAAACG TCTGGAAGAG
    GTGGTTCATC TAAAAGCTGA AAGGAAAACC GACAATCAGG AGATCGACAT CAAGATCCAA
    TTGACGAAGA TTCTGCCTCC CAGCTCTGAC CTTTGTATCC CGTTTTACAA CGTGGTCTTA
    AGGAGAGTGA TGAAAATACT GGGGCTGAAG CTGGTGGGCA GAAACCATTA CGACCCCAAT
    GCGGTTGTGA TCCTGGGCAA ACACAGGCTT CAGGTGTGGC CTGGATACTC CACCAGTATT
    AAGCACACTG ACGGTGGCCT CTACCTGGTT GTGGATGTCT CGCATAAGGT GCTGCGAAAC
    GACTCTGTGC TGGATGTCAT GAATCTGATT TACCAAGGAA GCCGTGAGAG CTTTCAGGAT
    GAATGCACAA AGGAGTTTGT GGGTTCAATC GTCATCACAC GCTATAATAA CCGCACTTAC
    CGTATTGATG ACATCGAGTG GTCCAAGTCT CCCAAAGACA CTTTCACCTT GGCTGATGGA
    TCTGTGACCA CGTTTGTCGA TTACTACAGA AAGAACTATG GGATTACCAT AAAGGAACTG
    GACCAACCGC TGCTCATTCA TCGTCCAAAA GAGAGGTCCA GACCAGGAGG AAAGGTTATA
    ACAGGTGAAA TCCTTTTGTT GCCGGAGCTT TCCTTCATGA CTGGCATCCC TGAGAAAATG
    AGGAAAGACT TCAGGGCCAT GAAGGATCTG ACCATGCACA TCAATGTGGG AGCTCAGCAA
    CACACCCAGA GTCTCAAGCA GCTGCTGCAC AACATCAACA GCAACAATGA AGCTCTCTCA
    GAGCTGGGCC GCTGGGGCCT CAGCATCAGC CAGGAGATTC TAGTGACTCA AGGAAGAACT
    CTGCCCAGTG AGACCATCTG CTTGCATTCA GCCTCTTTTG TCACATCTCC GGCAGTAGAC
    TGGTCCAGAG AGTTGGTTCG AGACCCCTCC ATCAGCACTG TCCCTCTGAA TTGCTGGGCT
    GTTTTCTATC CCAGACGGGC AACCGATCAG GCTGAGGAGC TGGTGACCAC TTTTTCAAGG
    GTAGCTGGTC CTATGGGCAT GAGAGTGGAA CGACCCATCC GTGTAGAGTT GCGCGATGAT
    CGAACCGAGA CCTTCGTCAA GAGCATTCAC TCTCAACTCA CCAGCGAGCC TCGTGTACAG
    CTAGTCGTGT GCATCATGAC AGGAAACAGA GATGACCTGT ATAGTGCCAT CAAAAAGCTT
    TGCTGCATCC AGAGTCCAGT ACCATCACAG GCAATCAACG TTCGGACCAT CTCACAGCCG
    CAGAAGCTCC GCAGTGTTGC TCAAAAGATC CTTTTGCAGA TTAACTGCAA GCTTGGAGGA
    GAGCTGTGGA CGGTGAACGT TCCTCTGAAA TACTTGATGG TGATTGGAGT GGATGTTCAC
    CATGATACCA GCAAGAAGAG CAGATCTGTC ATGGGCTTCG TGGCAAGTCT CAACAGCATG
    TTGACCAAAT GGTACTCCAG GGTGACCTTC CAAATGCCTA ATGAGGAGAT TATCAACGGC
    TTTAGAGTGT GTCTTCTCGC TGCCCTGCAG AAATACTATG AGGTGAACCA TGCCTTCCCT
    GAGAAGATTG TCATCTATCG CGACGGTGTG TCTGACGGAC AGCTGAAGAC CGTGGAGCAC
    TACGAGATCC CTCAGATTCT GAAATGCTTC GAGACCATCC CAAACTACGA GCCTAAGCTT
    GCATTCATCG TGGTGCAGAA AAGAATCAGC ACAACCCTGT ACTCCTACGG GTCAGACCAC
    TTCGGGACAC CCTCGCCAGG AACTGTGCTG GACCACACTG TGACAAACAG AGACTGGGTG
    GACTTCTACC TAATGGCACA TTCCATTCGA CAGGGTTGTG GCCTGCCCAC TCATTACATA
    ACAGTGTACA ACACCGCAAA CCTCACCCCT GACCACCTGC AGAGGTTGAC CTTCAAGATG
    TGTCACCTGT ACTGGAACTG GCCTGGAACA ATACGCGTTC CTGCACCGTG CAAATATGCC
    CACAAACTGG CTTTCCTGTC TGGCCAGTAC CTCCATTCAG AGCCTGCAAT CCAGCTGTCA
    GAGAAACTGT TTTTTCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331982.1
    CDS95..3235
    Translation

    Target ORF information:

    RefSeq Version XM_021476307.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio piwi-like RNA-mediated gene silencing 2 (piwil2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476307.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATCCAA AACGACCAAC CTTTCCCAGT CCCCCAGGGG TCATCCGGGC ACCATGGCAG 
    CAGTCGACAG AGGATCAGAG TCAACTTTTA GATCAGCCCT CATTGGGACG GGCCAGAGGT
    CTTATCATGC CCATAGATGA ACCTCTTCCT GGACGAGGTA GAGCTTTTAG TGTACCCGGA
    GAAATGCCGG TGAGATTTGG TAGAGGGATA ACTCAGTCTA TAGCTGCTGA GCCTCTGGTG
    GGCATGGCTA GAGGTGTGAG GTTACCTATG GAAGAGGGAG GTTTTGGGCG AGGACGAGGT
    TTTTTGCTGC CCACTCCTGA GCCCACTGTT GGGATAGGGA GAGGGGCTGC TATTGGACCA
    GTGCCTACAC TGGACATTCA AAAAGCAGAG GTGGAGGAAA AGATGCCTGA GCTGCAAGCG
    GAGGTGGCTC CAACTGTTGC CAAGGTTGGT TCCCCTGGGA CTGGATCGTC ACTGGTGTCC
    ATGTTCAGGG GTCTGGGAAT TGAGCCAGGA AAGACATGGG GTAGAGGAGC TGCACCTGTT
    GGACGGGGTG CAGCAGGCGA TATGGGGGCA GATTTGCAGC CAAAACCCAC CATCATTGGA
    GCATCTCTGA CTCCAGAAAG AGAAGAGGTT CGCTCAGAAG AATCGATCAG TTTCCTGGGA
    AGAGGCTTTA CTGGTTTTGG CAGAGCAGCA ATGCCTCATA TGACTGTTGG GCGTGGACCG
    ATTGGACCAT TAAGTCCTTC ACCTTCTGTC GCTGCCCCTT TCTCTTTGAT TTCTGCTTCC
    TCTGCCTCTG AAGATGCCCC GGTTGCTCCA GGAACACCTC CTAAAGTTGA AGTCAAAATT
    GAGACAGTGA AGGAGCCTCT GCAGAAGATA GGAACTAAAG GCAGTCCCAT ACCCATTGGA
    TCAAACTACA TCCCTATCTG CTGCAAAAAC GATGCAGTGT TTCAGTACCA TGTTACTTTT
    ACGCCGAACG TTGAATCACT TAGCATGCGC TTCGGAATGA TGAAGGAGCA TCGGCCCACT
    ACTGGAGAAG TAGTGGCTTT TGACGGATCT ATCCTCTACC TGCCAAAACG TCTGGAAGAG
    GTGGTTCATC TAAAAGCTGA AAGGAAAACC GACAATCAGG AGATCGACAT CAAGATCCAA
    TTGACGAAGA TTCTGCCTCC CAGCTCTGAC CTTTGTATCC CGTTTTACAA CGTGGTCTTA
    AGGAGAGTGA TGAAAATACT GGGGCTGAAG CTGGTGGGCA GAAACCATTA CGACCCCAAT
    GCGGTTGTGA TCCTGGGCAA ACACAGGCTT CAGGTGTGGC CTGGATACTC CACCAGTATT
    AAGCACACTG ACGGTGGCCT CTACCTGGTT GTGGATGTCT CGCATAAGGT GCTGCGAAAC
    GACTCTGTGC TGGATGTCAT GAATCTGATT TACCAAGGAA GCCGTGAGAG CTTTCAGGAT
    GAATGCACAA AGGAGTTTGT GGGTTCAATC GTCATCACAC GCTATAATAA CCGCACTTAC
    CGTATTGATG ACATCGAGTG GTCCAAGTCT CCCAAAGACA CTTTCACCTT GGCTGATGGA
    TCTGTGACCA CGTTTGTCGA TTACTACAGA AAGAACTATG GGATTACCAT AAAGGAACTG
    GACCAACCGC TGCTCATTCA TCGTCCAAAA GAGAGGTCCA GACCAGGAGG AAAGGTTATA
    ACAGGTGAAA TCCTTTTGTT GCCGGAGCTT TCCTTCATGA CTGGCATCCC TGAGAAAATG
    AGGAAAGACT TCAGGGCCAT GAAGGATCTG ACCATGCACA TCAATGTGGG AGCTCAGCAA
    CACACCCAGA GTCTCAAGCA GCTGCTGCAC AACATCAACA GCAACAATGA AGCTCTCTCA
    GAGCTGGGCC GCTGGGGCCT CAGCATCAGC CAGGAGATTC TAGTGACTCA AGGAAGAACT
    CTGCCCAGTG AGACCATCTG CTTGCATTCA GCCTCTTTTG TCACATCTCC GGCAGTAGAC
    TGGTCCAGAG AGTTGGTTCG AGACCCCTCC ATCAGCACTG TCCCTCTGAA TTGCTGGGCT
    GTTTTCTATC CCAGACGGGC AACCGATCAG GCTGAGGAGC TGGTGACCAC TTTTTCAAGG
    GTAGCTGGTC CTATGGGCAT GAGAGTGGAA CGACCCATCC GTGTAGAGTT GCGCGATGAT
    CGAACCGAGA CCTTCGTCAA GAGCATTCAC TCTCAACTCA CCAGCGAGCC TCGTGTACAG
    CTAGTCGTGT GCATCATGAC AGGAAACAGA GATGACCTGT ATAGTGCCAT CAAAAAGCTT
    TGCTGCATCC AGAGTCCAGT ACCATCACAG GCAATCAACG TTCGGACCAT CTCACAGCCG
    CAGAAGCTCC GCAGTGTTGC TCAAAAGATC CTTTTGCAGA TTAACTGCAA GCTTGGAGGA
    GAGCTGTGGA CGGTGAACGT TCCTCTGAAA TACTTGATGG TGATTGGAGT GGATGTTCAC
    CATGATACCA GCAAGAAGAG CAGATCTGTC ATGGGCTTCG TGGCAAGTCT CAACAGCATG
    TTGACCAAAT GGTACTCCAG GGTGACCTTC CAAATGCCTA ATGAGGAGAT TATCAACGGC
    TTTAGAGTGT GTCTTCTCGC TGCCCTGCAG AAATACTATG AGGTGAACCA TGCCTTCCCT
    GAGAAGATTG TCATCTATCG CGACGGTGTG TCTGACGGAC AGCTGAAGAC CGTGGAGCAC
    TACGAGATCC CTCAGATTCT GAAATGCTTC GAGACCATCC CAAACTACGA GCCTAAGCTT
    GCATTCATCG TGGTGCAGAA AAGAATCAGC ACAACCCTGT ACTCCTACGG GTCAGACCAC
    TTCGGGACAC CCTCGCCAGG AACTGTGCTG GACCACACTG TGACAAACAG AGACTGGGTG
    GACTTCTACC TAATGGCACA TTCCATTCGA CAGGGTTGTG GCCTGCCCAC TCATTACATA
    ACAGTGTACA ACACCGCAAA CCTCACCCCT GACCACCTGC AGAGGTTGAC CTTCAAGATG
    TGTCACCTGT ACTGGAACTG GCCTGGAACA ATACGCGTTC CTGCACCGTG CAAATATGCC
    CACAAACTGG CTTTCCTGTC TGGCCAGTAC CTCCATTCAG AGCCTGCAAT CCAGCTGTCA
    GAGAAACTGT TTTTTCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.