med1 cDNA ORF clone, Danio rerio(zebrafish)

The following med1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the med1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa148437 XM_021480458.1
Latest version!
Danio rerio mediator complex subunit 1 (med1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa54499 XM_021480457.1
Latest version!
Danio rerio mediator complex subunit 1 (med1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa54499 XM_017358511.1
Latest version!
Danio rerio mediator complex subunit 1 (med1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa148437
    Clone ID Related Accession (Same CDS sequence) XM_021480458.1
    Accession Version XM_021480458.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5310bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAAATTC GGAAGATGGC GGCGGTGCAA AGCAGCAGTC CGGTCGCAGA CTCGTCCCAG 
    GGAGGCACAG CCGCGGGCTC CGAGCGTCCC CGAGCCGAGG ATGGATCTGA GTCTGAGAAA
    CAGAGTCGTA TAATGACACT GCTAGAAAGG CTCTATTGCA AACACAGCTC ATCCAGGTCC
    TGGCAGGAGA CCAGCAAAGT GATACGGCAG GCAATGGAAA AGCGTGGAGC TGTTAATACA
    GGTGGACACC AGCTATTGCT CAACTGTTTA GAGACACTGC AAAGGGCATT AAAAGTTTCT
    ACACTGCCAT CAATGACAGA TCGCCTGGAG TCCATTGCTC GACAAAACAT GTTGGGATCT
    CACCTGAGTC CTTCACATAC TGAATGCTAT ATCACCTCTG ATATGTTCTA CGTTGAGGTG
    CATATGGATA AAAGTGGAAA GCTCATGGAT GTAAAAGTGG CCCACCATGG TGAAAATCCT
    GTGAGTTGTC CTGAGCTGAT ACAGCATTTA CGAGACAAGA ATTTTGATGA GTTTTCCAAA
    CACCTGAAGG GTCTGGTCAA CCTGTACAAT CTCCCTGGAG ACAACAAATT AAAGACAAAG
    ATGTACTTGG CACTTCAGTC ACTGGAGCTA GACCTCACAA AGATGATGCA TATGTTCAGG
    ATTGCCACTA ATGCTAACAC TGTGGAGACC ATACTTCACG GAAGTGTGGG TCTGTTGACG
    TCGAGGAGTG GTGGCCATTT AGTATCTCTG CAGTGTTATG TTTCTCCAAA TGACATCTTT
    GAGGAGGAAA AAGCAGCCCA TCTGAACACT GATGTCAATA TCCCCCATAC GTTAGGTGTG
    AGTGTGTCAG TGACTGTTGA AGGCACAACA TCAGTTTACA AGCTTCCCAT TGCTCCACTA
    ATCACAGGAT CCCATCCAGT TGACAACAAA GGAACTCCAT CATTTTCTTC CATAACAAAT
    TCAAACTGTG TAGATTTGCC AGCGTGTTTC TTCCTGAAGA TGAACTGCTT GATGCCATTT
    TCTTTATCAT ACATTAAAAA AATGGGCAGT ACTACAGGCA TTCCTGTCTT TGAAACTGCG
    CCCACCTTGT CCCCACTATA TGATCTCATC ATTAAGAGTC AACTCCGTGA GGAGGGAGGT
    GCCAGCCCCC CTTCGGCTCA ATCAATGCGT TTTTATGCAT CTTTACCAGG ACAGCAGCAC
    TGTTATTTCC TAAATGGTGA TGCTCCAGTA CAAGATGGAA GGTCTCTTCA AGGAGCTTTG
    GTTTCCAAGA TTCCCTTCAG ACATCCTGCT CAGGTGCCTG CTCTCCTTGA CATTATAAGA
    CATCAGGCTG CTTACAACAC GTTGATTGGC AGTTGCGTTA AAAAGGCCTG CATTAAGGAA
    GATACTCCTG GACTGCTGCA GTTTGAAGTG TGCCCACTAA CAGACTCAAG CTTTAGTGTG
    TCCTTTCAAC ACCCTGTAAA TGAGTCACTT GTTTGTGTGG TGATGGAGGT CATTGATTCG
    ATGCAGGTAG CCTGCAAACT CTACAAGGGT TTATCTGATG CTTTGATCTG CACTGATGAC
    TTCATTACTA AAGTAGTCCA AAGATGCATG TCTATTCCGG TGACTATGCG TGCCATACGA
    CGCAAGGCAG AGACCATTCA GGCAGACACA CCAGCGTTGT CCCTTATAGC TGAAACAGTG
    GAAATTATGG TGAAGAAGAA CTTGCCCCCA ACTGGCAGCC CAGGTTACAT GGGAATGGGT
    ACAGGGCCAG ATGGAAGTAA CCCAATGGGT CTCCCTTTAA ATAGTGGAAA CAACAGTGTT
    GTGTGTGTAG GGGGGAATGG ATCTTTCCAA GGTCCAATCA CCTCAATGTT CAACATGGGA
    CGTACTGGTC AAGGAAGCTC GTGCATTGAT ACCATGGGAC AGGTTGGAGG TCAGCAGCAA
    GCAATGCAGC AGCAACATCA ACAATCTCAG AGCCATGGGT CAGATGACTT TAACAAAGTC
    ACTCAGAATC CAATTCTAAC CAGCCTTTTG CAAATAACAG GCAGTGTTGG GTCCAGTCCT
    ACTCCCCAGC AACCTCAACC CCACCAAACA CCTCCAACAA CCACCTCACC AGCAAGCAAC
    ACCAAAAACC ACCCTATGCT AATGAACCTA CTGAAGGATA ATCCATCACA GGATTTTGCT
    GCATTGTACA GCAGTAGCCC TCTAGAAAGA CAGAACTCAT CTGGGTCTCC ACGGACTGAC
    AACCAAGGCC AGCCTTGCTC TGGGACCAAG GGCAAAAAAA AGCGTCTGAG GGGCTCTGAT
    AAAGGAGTAA TGGGAGCAGC TGGGGGAATG AGTATGAAAC AATCTCAGCA GCAAGTGGTA
    GACATGACAC AACATCATCA CCTCACAAGC CACTCTGAAG ATGATTTCCA CCGAGAGCTT
    TTTTCTATGG ATGTAGATGC CTCCCAAAAC CCGATTTTTG ATGTTAGTCT TCCAGGTGAT
    GGACTTGACA CTCCACACAG TATCACTCCA GCTCCAAGTC AGTGTGGAAC ACCACCTCCA
    GGTCCCGGAA TGCAATACCA CCCACAATGT CATGTCCAGC CTCAGATACA AGTCCAGTCA
    CAATCACAAG GATCCATTCC TCGAATGGTC CGCCTCTCCA GCTCTGACAG TATTGGACCT
    GATATTAATG ACATTCTTTC TGATATTCCT GATCAAGCCA ACAAAGGAAG CAATATCGGT
    CATGGACAGC ATCTTATGAC TAGTGAAGAT GGAGGTTCCT TGGGAACCCC AGTCCGGGAC
    TCTTCCAGCT CTGGCCAGGG TAGTACAGTG TTTGAGGCAG ACCTGTTTAA TACAAATAGT
    AATGAAAATC CCTTTACTGA TGCAGCAGAT TTGATTGCAG AAGCTGCTAC TGCAACAACA
    CCCAATAGTG ATACTTCCTC AACTAACTTC TTCCCAGACA CAGACTTCAA CCCAGAGCTT
    TTATCTGGGC AGGGAAGTTT TTCACAGACC TACTTTGAAG ACAGCTCTCC CAGCCCTGAT
    CCAGATCTTG AATTGGTTAA AAGTTTCAGT GGAGGAAGTC AGCAGAACAC TCCTACAGGC
    ACACCTCAGA ATCCTGCCTC ACATGGCCAA AATACTCCTG AGACTTCATT AAAAGACCCT
    TTTGAAATTG GTATTTTTGG TGGAAACGGT GGGGGAGGAA AACCATTAAT GGCTCCAGCA
    TCAGATATTG CAGACTCGCA CCATGCTCAT ACTGGTGGAG GTCAAAGTCC ACTTATTGGA
    ATTGTTGTTG CAGGTAATGA TTTTAAAAAT GTAGATGCAA AGGCTAAGCA GCAGCAGATG
    AGGACAAAAG AAGATCATGT TGGAGGCAGT TCTGGACAAG GAATGGTTAG TGCATGCTCA
    TCTGAAATGA AGCAGGTAAA GCGCAGTCGT ACTCCTTCAA GTGAGGGAAA ATCAAAAGAC
    AAACCAGCTA AACGCAAGAA GCTGGAGCCA GATGCTAAGT CACCTTCACA TAGTGCAGGG
    GGTCGACCTT ACACTCCACC TTGTGGAAGT GCCACTTCTG TAGGTTTAGT AAGTACTGGT
    GGATCTAAGT CCCCTGGGAG TGTAGGTAGG TCTCAGACCC CACCAGGAGG TGCTACTCCA
    CCAATCCCTA AAATCACTAT ACAAATACCC AGAGCTTTAA CAGGAGGAAA AACATCATCT
    CATGGAGGTT ACACATCTAG TAGCTCAACA AGTGGTGGTA CTGGTAATAC AATTGGCACA
    AGCAATAGCA AAAGCCATCA TTCCTCTTCA TCTGGGAAGA TAAAAAGTAG TAAACTTGAA
    AGTTCTGTTG GGCAGGGCAA TATTGCAAAA CAATCAAGTG CTGCTGGTAC TGGAAGTGGT
    ATACCTTCTC AATCTAAAGT GTCTTCCATG GGCATGGGCA CAGGCAAGCC ATCTTCTTCT
    CCTATTACGA AGCATGGTTT GTCAAGCTCG GGAGGTGGTG GCAGCAGTGG ATCAGGAAGT
    GGAATTAAAA ACAAACCTCA AGGTGGTAAG CCCCCTGGCT CACTCATGAA CCCCAACATC
    AAGCCCAACA TATCACCCTC GCACTCTCGT TCTGGAGGTT CTGACAAGCT GTCATCTCCC
    ATGAAACAAG GCCAAGTACC TGGGACCCCA CCCTCTTCTA AGGCTAAATC CCCTATTGGC
    TCAGGCAGTG GATCAGGGGG CAATAAGTGC TCTTCGGGTG GTGGAGTTGG ATCAAAATCA
    TTAAGCAGCA GTTCATCCAA CAGCGCTTCT ACATCCTGTG CTTCCTCCAC TGGCTCCTCG
    TCATCTGGCT CTATGCCCTT TTCTTCAAAT GGTCAGTCAC AATATGGAAG CAGTGGTGGA
    AATTGCAGTG GTGGAGGGGG TGGTGGTGGC TGTGGAAACA ACCCAAATGC AAAGGGTAAG
    TCCCCAAGCC GAAACAAAAA GCCATCTCTT ACAGCTGTCA TAGACAAACT CAAGAGTGTG
    GGAAGTGGGG GTAATGGTGG GGAGGAAATT GAAAGTTGTG GTGGTTCAAG TGGAGTAGGG
    CAGCAAAATA CTGTTCCTAC ATCCTCAAAG CATTCTATGT CCTCCCAAGG AGGTGAGTTT
    TTGAGCAAAC GAGACAAGAT AGACAAAGAT GGGAAATCCA AGATGTCTGG ATCAGGGGGG
    AATTCAAGTG ACAAAAAGAT GATTGATGCC AAAAGTAACA CAATGACTGG TACTGGTGTG
    GCAAAGATTA TTATCAGCAA GCCAGAAGGA GGCTCTCCAA GCATTAAATC TAAAGTAACA
    CTTCAGAAAC CTGGGGAAGG TTCTGGTGAT GGTGGCATTC GTTCCCAACA CTCAGGCCAC
    AAAGCATCAC CTCTATTTAG TGGTTCTACA CCAAAACATG ACCGCAGCTC CCCAAGCCAC
    AGCCGTTCTC CTGGGTGTAC ACCTATGAAT CCAGACAGTG AGAGTGAGTC AGGTAGCAGT
    TCAGTTGCTG AAAAGTCACA TCAGAATAGT CCTAGCTCTG ATGATGACCA AACTATGAGA
    TTACACCAAC CAATTCAAGA CTACATGAGC TCCATATCTA TTAGCCAGAG TGAAAAACAC
    AAAAAGCATA AAAAAGAGAA AAAGAAGCAG AAAGATCGTG AAAGAGATAA GGAGAGAGAT
    AAGGAAAAAG AAAAGAAGAA GTCAATGTCT TGTGGGCCCT CACACACAGT AAAGACAGAT
    AATTGGTCTA GATCTCCTAT ATCTTCAGAA ACATCAGTGT CATTGCTTAG CTCTGAGCGT
    TCATCACGAC CAAGTCCTGT GTACATGCAT ACAGAGGATG ATGATCTCAT GGACTCTGCC
    CTAACAGGCA ATCTAGAACC TTTCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336133.1
    CDS202..5511
    Translation

    Target ORF information:

    RefSeq Version XM_021480458.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mediator complex subunit 1 (med1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480458.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAAATTC GGAAGATGGC GGCGGTGCAA AGCAGCAGTC CGGTCGCAGA CTCGTCCCAG 
    GGAGGCACAG CCGCGGGCTC CGAGCGTCCC CGAGCCGAGG ATGGATCTGA GTCTGAGAAA
    CAGAGTCGTA TAATGACACT GCTAGAAAGG CTCTATTGCA AACACAGCTC ATCCAGGTCC
    TGGCAGGAGA CCAGCAAAGT GATACGGCAG GCAATGGAAA AGCGTGGAGC TGTTAATACA
    GGTGGACACC AGCTATTGCT CAACTGTTTA GAGACACTGC AAAGGGCATT AAAAGTTTCT
    ACACTGCCAT CAATGACAGA TCGCCTGGAG TCCATTGCTC GACAAAACAT GTTGGGATCT
    CACCTGAGTC CTTCACATAC TGAATGCTAT ATCACCTCTG ATATGTTCTA CGTTGAGGTG
    CATATGGATA AAAGTGGAAA GCTCATGGAT GTAAAAGTGG CCCACCATGG TGAAAATCCT
    GTGAGTTGTC CTGAGCTGAT ACAGCATTTA CGAGACAAGA ATTTTGATGA GTTTTCCAAA
    CACCTGAAGG GTCTGGTCAA CCTGTACAAT CTCCCTGGAG ACAACAAATT AAAGACAAAG
    ATGTACTTGG CACTTCAGTC ACTGGAGCTA GACCTCACAA AGATGATGCA TATGTTCAGG
    ATTGCCACTA ATGCTAACAC TGTGGAGACC ATACTTCACG GAAGTGTGGG TCTGTTGACG
    TCGAGGAGTG GTGGCCATTT AGTATCTCTG CAGTGTTATG TTTCTCCAAA TGACATCTTT
    GAGGAGGAAA AAGCAGCCCA TCTGAACACT GATGTCAATA TCCCCCATAC GTTAGGTGTG
    AGTGTGTCAG TGACTGTTGA AGGCACAACA TCAGTTTACA AGCTTCCCAT TGCTCCACTA
    ATCACAGGAT CCCATCCAGT TGACAACAAA GGAACTCCAT CATTTTCTTC CATAACAAAT
    TCAAACTGTG TAGATTTGCC AGCGTGTTTC TTCCTGAAGA TGAACTGCTT GATGCCATTT
    TCTTTATCAT ACATTAAAAA AATGGGCAGT ACTACAGGCA TTCCTGTCTT TGAAACTGCG
    CCCACCTTGT CCCCACTATA TGATCTCATC ATTAAGAGTC AACTCCGTGA GGAGGGAGGT
    GCCAGCCCCC CTTCGGCTCA ATCAATGCGT TTTTATGCAT CTTTACCAGG ACAGCAGCAC
    TGTTATTTCC TAAATGGTGA TGCTCCAGTA CAAGATGGAA GGTCTCTTCA AGGAGCTTTG
    GTTTCCAAGA TTCCCTTCAG ACATCCTGCT CAGGTGCCTG CTCTCCTTGA CATTATAAGA
    CATCAGGCTG CTTACAACAC GTTGATTGGC AGTTGCGTTA AAAAGGCCTG CATTAAGGAA
    GATACTCCTG GACTGCTGCA GTTTGAAGTG TGCCCACTAA CAGACTCAAG CTTTAGTGTG
    TCCTTTCAAC ACCCTGTAAA TGAGTCACTT GTTTGTGTGG TGATGGAGGT CATTGATTCG
    ATGCAGGTAG CCTGCAAACT CTACAAGGGT TTATCTGATG CTTTGATCTG CACTGATGAC
    TTCATTACTA AAGTAGTCCA AAGATGCATG TCTATTCCGG TGACTATGCG TGCCATACGA
    CGCAAGGCAG AGACCATTCA GGCAGACACA CCAGCGTTGT CCCTTATAGC TGAAACAGTG
    GAAATTATGG TGAAGAAGAA CTTGCCCCCA ACTGGCAGCC CAGGTTACAT GGGAATGGGT
    ACAGGGCCAG ATGGAAGTAA CCCAATGGGT CTCCCTTTAA ATAGTGGAAA CAACAGTGTT
    GTGTGTGTAG GGGGGAATGG ATCTTTCCAA GGTCCAATCA CCTCAATGTT CAACATGGGA
    CGTACTGGTC AAGGAAGCTC GTGCATTGAT ACCATGGGAC AGGTTGGAGG TCAGCAGCAA
    GCAATGCAGC AGCAACATCA ACAATCTCAG AGCCATGGGT CAGATGACTT TAACAAAGTC
    ACTCAGAATC CAATTCTAAC CAGCCTTTTG CAAATAACAG GCAGTGTTGG GTCCAGTCCT
    ACTCCCCAGC AACCTCAACC CCACCAAACA CCTCCAACAA CCACCTCACC AGCAAGCAAC
    ACCAAAAACC ACCCTATGCT AATGAACCTA CTGAAGGATA ATCCATCACA GGATTTTGCT
    GCATTGTACA GCAGTAGCCC TCTAGAAAGA CAGAACTCAT CTGGGTCTCC ACGGACTGAC
    AACCAAGGCC AGCCTTGCTC TGGGACCAAG GGCAAAAAAA AGCGTCTGAG GGGCTCTGAT
    AAAGGAGTAA TGGGAGCAGC TGGGGGAATG AGTATGAAAC AATCTCAGCA GCAAGTGGTA
    GACATGACAC AACATCATCA CCTCACAAGC CACTCTGAAG ATGATTTCCA CCGAGAGCTT
    TTTTCTATGG ATGTAGATGC CTCCCAAAAC CCGATTTTTG ATGTTAGTCT TCCAGGTGAT
    GGACTTGACA CTCCACACAG TATCACTCCA GCTCCAAGTC AGTGTGGAAC ACCACCTCCA
    GGTCCCGGAA TGCAATACCA CCCACAATGT CATGTCCAGC CTCAGATACA AGTCCAGTCA
    CAATCACAAG GATCCATTCC TCGAATGGTC CGCCTCTCCA GCTCTGACAG TATTGGACCT
    GATATTAATG ACATTCTTTC TGATATTCCT GATCAAGCCA ACAAAGGAAG CAATATCGGT
    CATGGACAGC ATCTTATGAC TAGTGAAGAT GGAGGTTCCT TGGGAACCCC AGTCCGGGAC
    TCTTCCAGCT CTGGCCAGGG TAGTACAGTG TTTGAGGCAG ACCTGTTTAA TACAAATAGT
    AATGAAAATC CCTTTACTGA TGCAGCAGAT TTGATTGCAG AAGCTGCTAC TGCAACAACA
    CCCAATAGTG ATACTTCCTC AACTAACTTC TTCCCAGACA CAGACTTCAA CCCAGAGCTT
    TTATCTGGGC AGGGAAGTTT TTCACAGACC TACTTTGAAG ACAGCTCTCC CAGCCCTGAT
    CCAGATCTTG AATTGGTTAA AAGTTTCAGT GGAGGAAGTC AGCAGAACAC TCCTACAGGC
    ACACCTCAGA ATCCTGCCTC ACATGGCCAA AATACTCCTG AGACTTCATT AAAAGACCCT
    TTTGAAATTG GTATTTTTGG TGGAAACGGT GGGGGAGGAA AACCATTAAT GGCTCCAGCA
    TCAGATATTG CAGACTCGCA CCATGCTCAT ACTGGTGGAG GTCAAAGTCC ACTTATTGGA
    ATTGTTGTTG CAGGTAATGA TTTTAAAAAT GTAGATGCAA AGGCTAAGCA GCAGCAGATG
    AGGACAAAAG AAGATCATGT TGGAGGCAGT TCTGGACAAG GAATGGTTAG TGCATGCTCA
    TCTGAAATGA AGCAGGTAAA GCGCAGTCGT ACTCCTTCAA GTGAGGGAAA ATCAAAAGAC
    AAACCAGCTA AACGCAAGAA GCTGGAGCCA GATGCTAAGT CACCTTCACA TAGTGCAGGG
    GGTCGACCTT ACACTCCACC TTGTGGAAGT GCCACTTCTG TAGGTTTAGT AAGTACTGGT
    GGATCTAAGT CCCCTGGGAG TGTAGGTAGG TCTCAGACCC CACCAGGAGG TGCTACTCCA
    CCAATCCCTA AAATCACTAT ACAAATACCC AGAGCTTTAA CAGGAGGAAA AACATCATCT
    CATGGAGGTT ACACATCTAG TAGCTCAACA AGTGGTGGTA CTGGTAATAC AATTGGCACA
    AGCAATAGCA AAAGCCATCA TTCCTCTTCA TCTGGGAAGA TAAAAAGTAG TAAACTTGAA
    AGTTCTGTTG GGCAGGGCAA TATTGCAAAA CAATCAAGTG CTGCTGGTAC TGGAAGTGGT
    ATACCTTCTC AATCTAAAGT GTCTTCCATG GGCATGGGCA CAGGCAAGCC ATCTTCTTCT
    CCTATTACGA AGCATGGTTT GTCAAGCTCG GGAGGTGGTG GCAGCAGTGG ATCAGGAAGT
    GGAATTAAAA ACAAACCTCA AGGTGGTAAG CCCCCTGGCT CACTCATGAA CCCCAACATC
    AAGCCCAACA TATCACCCTC GCACTCTCGT TCTGGAGGTT CTGACAAGCT GTCATCTCCC
    ATGAAACAAG GCCAAGTACC TGGGACCCCA CCCTCTTCTA AGGCTAAATC CCCTATTGGC
    TCAGGCAGTG GATCAGGGGG CAATAAGTGC TCTTCGGGTG GTGGAGTTGG ATCAAAATCA
    TTAAGCAGCA GTTCATCCAA CAGCGCTTCT ACATCCTGTG CTTCCTCCAC TGGCTCCTCG
    TCATCTGGCT CTATGCCCTT TTCTTCAAAT GGTCAGTCAC AATATGGAAG CAGTGGTGGA
    AATTGCAGTG GTGGAGGGGG TGGTGGTGGC TGTGGAAACA ACCCAAATGC AAAGGGTAAG
    TCCCCAAGCC GAAACAAAAA GCCATCTCTT ACAGCTGTCA TAGACAAACT CAAGAGTGTG
    GGAAGTGGGG GTAATGGTGG GGAGGAAATT GAAAGTTGTG GTGGTTCAAG TGGAGTAGGG
    CAGCAAAATA CTGTTCCTAC ATCCTCAAAG CATTCTATGT CCTCCCAAGG AGGTGAGTTT
    TTGAGCAAAC GAGACAAGAT AGACAAAGAT GGGAAATCCA AGATGTCTGG ATCAGGGGGG
    AATTCAAGTG ACAAAAAGAT GATTGATGCC AAAAGTAACA CAATGACTGG TACTGGTGTG
    GCAAAGATTA TTATCAGCAA GCCAGAAGGA GGCTCTCCAA GCATTAAATC TAAAGTAACA
    CTTCAGAAAC CTGGGGAAGG TTCTGGTGAT GGTGGCATTC GTTCCCAACA CTCAGGCCAC
    AAAGCATCAC CTCTATTTAG TGGTTCTACA CCAAAACATG ACCGCAGCTC CCCAAGCCAC
    AGCCGTTCTC CTGGGTGTAC ACCTATGAAT CCAGACAGTG AGAGTGAGTC AGGTAGCAGT
    TCAGTTGCTG AAAAGTCACA TCAGAATAGT CCTAGCTCTG ATGATGACCA AACTATGAGA
    TTACACCAAC CAATTCAAGA CTACATGAGC TCCATATCTA TTAGCCAGAG TGAAAAACAC
    AAAAAGCATA AAAAAGAGAA AAAGAAGCAG AAAGATCGTG AAAGAGATAA GGAGAGAGAT
    AAGGAAAAAG AAAAGAAGAA GTCAATGTCT TGTGGGCCCT CACACACAGT AAAGACAGAT
    AATTGGTCTA GATCTCCTAT ATCTTCAGAA ACATCAGTGT CATTGCTTAG CTCTGAGCGT
    TCATCACGAC CAAGTCCTGT GTACATGCAT ACAGAGGATG ATGATCTCAT GGACTCTGCC
    CTAACAGGCA ATCTAGAACC TTTCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54499
    Clone ID Related Accession (Same CDS sequence) XM_021480457.1 , XM_017358511.1
    Accession Version XM_021480457.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mediator of RNA polymerase II transcription subunit 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAAATTC GGAAGATGGC GGCGGTGCAA AGCAGCAGTC CGGTCGCAGA CTCGTCCCAG 
    GGAGGCACAG CCGCGGGCTC CGAGCGTCCC CGAGCCGAGG ATGGATCTGA GTCTGAGAAA
    CAGAGTCGTA TAATGACACT GCTAGAAAGG CTCTATTGCA AACACAGCTC ATCCAGGTCC
    TGGCAGGAGA CCAGCAAAGT GATACGGCAG GCAATGGAAA AGCGTGGAGC TGTTAATACA
    GGTGGACACC AGCTATTGCT CAACTGTTTA GAGACACTGC AAAGGGCATT AAAAGTTTCT
    ACACTGCCAT CAATGACAGA TCGCCTGGAG TCCATTGCTC GACAAAACAT GTTGGGATCT
    CACCTGAGTC CTTCACATAC TGAATGCTAT ATCACCTCTG ATATGTTCTA CGTTGAGGTG
    CATATGGATA AAAGTGGAAA GCTCATGGAT GTAAAAGTGG CCCACCATGG TGAAAATCCT
    GTGAGTTGTC CTGAGCTGAT ACAGCATTTA CGAGACAAGA ATTTTGATGA GTTTTCCAAA
    CACCTGAAGG GTCTGGTCAA CCTGTACAAT CTCCCTGGAG ACAACAAATT AAAGACAAAG
    ATGTACTTGG CACTTCAGTC ACTGGAGCTA GACCTCACAA AGATGATGCA TATGTTCAGG
    ATTGCCACTA ATGCTAACAC TGTGGAGACC ATACTTCACG GAAGTGTGGG TCTGTTGACG
    TCGAGGAGTG GTGGCCATTT AGTATCTCTG CAGTGTTATG TTTCTCCAAA TGACATCTTT
    GAGGAGGAAA AAGCAGCCCA TCTGAACACT GATGTCAATA TCCCCCATAC GTTAGGTGTG
    AGTGTGTCAG TGACTGTTGA AGGCACAACA TCAGTTTACA AGCTTCCCAT TGCTCCACTA
    ATCACAGGAT CCCATCCAGT TGACAACAAA GGAACTCCAT CATTTTCTTC CATAACAAAT
    TCAAACTGTG TAGATTTGCC AGCGTGTTTC TTCCTGAAGA TGAACTGCTT GATGCCATTT
    TCTTTATCAT ACATTAAAAA AATGGGCAGT ACTACAGGCA TTCCTGTCTT TGAAACTGCG
    CCCACCTTGT CCCCACTATA TGATCTCATC ATTAAGAGTC AACTCCGTGA GGAGGGAGGT
    GCCAGCCCCC CTTCGGCTCA ATCAATGCGT TTTTATGCAT CTTTACCAGG ACAGCAGCAC
    TGTTATTTCC TAAATGGTGA TGCTCCAGTA CAAGATGGAA GGTCTCTTCA AGGAGCTTTG
    GTTTCCAAGA TTCCCTTCAG ACATCCTGCT CAGGTGCCTG CTCTCCTTGA CATTATAAGA
    CATCAGGCTG CTTACAACAC GTTGATTGGC AGTTGCGTTA AAAAGGCCTG CATTAAGGAA
    GCAGATACTC CTGGACTGCT GCAGTTTGAA GTGTGCCCAC TAACAGACTC AAGCTTTAGT
    GTGTCCTTTC AACACCCTGT AAATGAGTCA CTTGTTTGTG TGGTGATGGA GGTCATTGAT
    TCGATGCAGG TAGCCTGCAA ACTCTACAAG GGTTTATCTG ATGCTTTGAT CTGCACTGAT
    GACTTCATTA CTAAAGTAGT CCAAAGATGC ATGTCTATTC CGGTGACTAT GCGTGCCATA
    CGACGCAAGG CAGAGACCAT TCAGGCAGAC ACACCAGCGT TGTCCCTTAT AGCTGAAACA
    GTGGAAATTA TGGTGAAGAA GAACTTGCCC CCAACTGGCA GCCCAGGTTA CATGGGAATG
    GGTACAGGGC CAGATGGAAG TAACCCAATG GGTCTCCCTT TAAATAGTGG AAACAACAGT
    GTTGTGTGTG TAGGGGGGAA TGGATCTTTC CAAGGTCCAA TCACCTCAAT GTTCAACATG
    GGACGTACTG GTCAAGGAAG CTCGTGCATT GATACCATGG GACAGGTTGG AGGTCAGCAG
    CAAGCAATGC AGCAGCAACA TCAACAATCT CAGAGCCATG GGTCAGATGA CTTTAACAAA
    GTCACTCAGA ATCCAATTCT AACCAGCCTT TTGCAAATAA CAGGCAGTGT TGGGTCCAGT
    CCTACTCCCC AGCAACCTCA ACCCCACCAA ACACCTCCAA CAACCACCTC ACCAGCAAGC
    AACACCAAAA ACCACCCTAT GCTAATGAAC CTACTGAAGG ATAATCCATC ACAGGATTTT
    GCTGCATTGT ACAGCAGTAG CCCTCTAGAA AGACAGAACT CATCTGGGTC TCCACGGACT
    GACAACCAAG GCCAGCCTTG CTCTGGGACC AAGGGCAAAA AAAAGCGTCT GAGGGGCTCT
    GATAAAGGAG TAATGGGAGC AGCTGGGGGA ATGAGTATGA AACAATCTCA GCAGCAAGTG
    GTAGACATGA CACAACATCA TCACCTCACA AGCCACTCTG AAGATGATTT CCACCGAGAG
    CTTTTTTCTA TGGATGTAGA TGCCTCCCAA AACCCGATTT TTGATGTTAG TCTTCCAGGT
    GATGGACTTG ACACTCCACA CAGTATCACT CCAGCTCCAA GTCAGTGTGG AACACCACCT
    CCAGGTCCCG GAATGCAATA CCACCCACAA TGTCATGTCC AGCCTCAGAT ACAAGTCCAG
    TCACAATCAC AAGGATCCAT TCCTCGAATG GTCCGCCTCT CCAGCTCTGA CAGTATTGGA
    CCTGATATTA ATGACATTCT TTCTGATATT CCTGATCAAG CCAACAAAGG AAGCAATATC
    GGTCATGGAC AGCATCTTAT GACTAGTGAA GATGGAGGTT CCTTGGGAAC CCCAGTCCGG
    GACTCTTCCA GCTCTGGCCA GGGTAGTACA GTGTTTGAGG CAGACCTGTT TAATACAAAT
    AGTAATGAAA ATCCCTTTAC TGATGCAGCA GATTTGATTG CAGAAGCTGC TACTGCAACA
    ACACCCAATA GTGATACTTC CTCAACTAAC TTCTTCCCAG ACACAGACTT CAACCCAGAG
    CTTTTATCTG GGCAGGGAAG TTTTTCACAG ACCTACTTTG AAGACAGCTC TCCCAGCCCT
    GATCCAGATC TTGAATTGGT TAAAAGTTTC AGTGGAGGAA GTCAGCAGAA CACTCCTACA
    GGCACACCTC AGAATCCTGC CTCACATGGC CAAAATACTC CTGAGACTTC ATTAAAAGAC
    CCTTTTGAAA TTGGTATTTT TGGTGGAAAC GGTGGGGGAG GAAAACCATT AATGGCTCCA
    GCATCAGATA TTGCAGACTC GCACCATGCT CATACTGGTG GAGGTCAAAG TCCACTTATT
    GGAATTGTTG TTGCAGGTAA TGATTTTAAA AATGTAGATG CAAAGGCTAA GCAGCAGCAG
    ATGAGGACAA AAGAAGATCA TGTTGGAGGC AGTTCTGGAC AAGGAATGGT TAGTGCATGC
    TCATCTGAAA TGAAGCAGGT AAAGCGCAGT CGTACTCCTT CAAGTGAGGG AAAATCAAAA
    GACAAACCAG CTAAACGCAA GAAGCTGGAG CCAGATGCTA AGTCACCTTC ACATAGTGCA
    GGGGGTCGAC CTTACACTCC ACCTTGTGGA AGTGCCACTT CTGTAGGTTT AGTAAGTACT
    GGTGGATCTA AGTCCCCTGG GAGTGTAGGT AGGTCTCAGA CCCCACCAGG AGGTGCTACT
    CCACCAATCC CTAAAATCAC TATACAAATA CCCAGAGCTT TAACAGGAGG AAAAACATCA
    TCTCATGGAG GTTACACATC TAGTAGCTCA ACAAGTGGTG GTACTGGTAA TACAATTGGC
    ACAAGCAATA GCAAAAGCCA TCATTCCTCT TCATCTGGGA AGATAAAAAG TAGTAAACTT
    GAAAGTTCTG TTGGGCAGGG CAATATTGCA AAACAATCAA GTGCTGCTGG TACTGGAAGT
    GGTATACCTT CTCAATCTAA AGTGTCTTCC ATGGGCATGG GCACAGGCAA GCCATCTTCT
    TCTCCTATTA CGAAGCATGG TTTGTCAAGC TCGGGAGGTG GTGGCAGCAG TGGATCAGGA
    AGTGGAATTA AAAACAAACC TCAAGGTGGT AAGCCCCCTG GCTCACTCAT GAACCCCAAC
    ATCAAGCCCA ACATATCACC CTCGCACTCT CGTTCTGGAG GTTCTGACAA GCTGTCATCT
    CCCATGAAAC AAGGCCAAGT ACCTGGGACC CCACCCTCTT CTAAGGCTAA ATCCCCTATT
    GGCTCAGGCA GTGGATCAGG GGGCAATAAG TGCTCTTCGG GTGGTGGAGT TGGATCAAAA
    TCATTAAGCA GCAGTTCATC CAACAGCGCT TCTACATCCT GTGCTTCCTC CACTGGCTCC
    TCGTCATCTG GCTCTATGCC CTTTTCTTCA AATGGTCAGT CACAATATGG AAGCAGTGGT
    GGAAATTGCA GTGGTGGAGG GGGTGGTGGT GGCTGTGGAA ACAACCCAAA TGCAAAGGGT
    AAGTCCCCAA GCCGAAACAA AAAGCCATCT CTTACAGCTG TCATAGACAA ACTCAAGAGT
    GTGGGAAGTG GGGGTAATGG TGGGGAGGAA ATTGAAAGTT GTGGTGGTTC AAGTGGAGTA
    GGGCAGCAAA ATACTGTTCC TACATCCTCA AAGCATTCTA TGTCCTCCCA AGGAGGTGAG
    TTTTTGAGCA AACGAGACAA GATAGACAAA GATGGGAAAT CCAAGATGTC TGGATCAGGG
    GGGAATTCAA GTGACAAAAA GATGATTGAT GCCAAAAGTA ACACAATGAC TGGTACTGGT
    GTGGCAAAGA TTATTATCAG CAAGCCAGAA GGAGGCTCTC CAAGCATTAA ATCTAAAGTA
    ACACTTCAGA AACCTGGGGA AGGTTCTGGT GATGGTGGCA TTCGTTCCCA ACACTCAGGC
    CACAAAGCAT CACCTCTATT TAGTGGTTCT ACACCAAAAC ATGACCGCAG CTCCCCAAGC
    CACAGCCGTT CTCCTGGGTG TACACCTATG AATCCAGACA GTGAGAGTGA GTCAGGTAGC
    AGTTCAGTTG CTGAAAAGTC ACATCAGAAT AGTCCTAGCT CTGATGATGA CCAAACTATG
    AGATTACACC AACCAATTCA AGACTACATG AGCTCCATAT CTATTAGCCA GAGTGAAAAA
    CACAAAAAGC ATAAAAAAGA GAAAAAGAAG CAGAAAGATC GTGAAAGAGA TAAGGAGAGA
    GATAAGGAAA AAGAAAAGAA GAAGTCAATG TCTTGTGGGC CCTCACACAC AGTAAAGACA
    GATAATTGGT CTAGATCTCC TATATCTTCA GAAACATCAG TGTCATTGCT TAGCTCTGAG
    CGTTCATCAC GACCAAGTCC TGTGTACATG CATACAGAGG ATGATGATCT CATGGACTCT
    GCCCTAACAG GCAATCTAGA ACCTTTCAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336132.1
    CDS202..5514
    Translation

    Target ORF information:

    RefSeq Version XM_021480457.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mediator complex subunit 1 (med1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480457.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAAATTC GGAAGATGGC GGCGGTGCAA AGCAGCAGTC CGGTCGCAGA CTCGTCCCAG 
    GGAGGCACAG CCGCGGGCTC CGAGCGTCCC CGAGCCGAGG ATGGATCTGA GTCTGAGAAA
    CAGAGTCGTA TAATGACACT GCTAGAAAGG CTCTATTGCA AACACAGCTC ATCCAGGTCC
    TGGCAGGAGA CCAGCAAAGT GATACGGCAG GCAATGGAAA AGCGTGGAGC TGTTAATACA
    GGTGGACACC AGCTATTGCT CAACTGTTTA GAGACACTGC AAAGGGCATT AAAAGTTTCT
    ACACTGCCAT CAATGACAGA TCGCCTGGAG TCCATTGCTC GACAAAACAT GTTGGGATCT
    CACCTGAGTC CTTCACATAC TGAATGCTAT ATCACCTCTG ATATGTTCTA CGTTGAGGTG
    CATATGGATA AAAGTGGAAA GCTCATGGAT GTAAAAGTGG CCCACCATGG TGAAAATCCT
    GTGAGTTGTC CTGAGCTGAT ACAGCATTTA CGAGACAAGA ATTTTGATGA GTTTTCCAAA
    CACCTGAAGG GTCTGGTCAA CCTGTACAAT CTCCCTGGAG ACAACAAATT AAAGACAAAG
    ATGTACTTGG CACTTCAGTC ACTGGAGCTA GACCTCACAA AGATGATGCA TATGTTCAGG
    ATTGCCACTA ATGCTAACAC TGTGGAGACC ATACTTCACG GAAGTGTGGG TCTGTTGACG
    TCGAGGAGTG GTGGCCATTT AGTATCTCTG CAGTGTTATG TTTCTCCAAA TGACATCTTT
    GAGGAGGAAA AAGCAGCCCA TCTGAACACT GATGTCAATA TCCCCCATAC GTTAGGTGTG
    AGTGTGTCAG TGACTGTTGA AGGCACAACA TCAGTTTACA AGCTTCCCAT TGCTCCACTA
    ATCACAGGAT CCCATCCAGT TGACAACAAA GGAACTCCAT CATTTTCTTC CATAACAAAT
    TCAAACTGTG TAGATTTGCC AGCGTGTTTC TTCCTGAAGA TGAACTGCTT GATGCCATTT
    TCTTTATCAT ACATTAAAAA AATGGGCAGT ACTACAGGCA TTCCTGTCTT TGAAACTGCG
    CCCACCTTGT CCCCACTATA TGATCTCATC ATTAAGAGTC AACTCCGTGA GGAGGGAGGT
    GCCAGCCCCC CTTCGGCTCA ATCAATGCGT TTTTATGCAT CTTTACCAGG ACAGCAGCAC
    TGTTATTTCC TAAATGGTGA TGCTCCAGTA CAAGATGGAA GGTCTCTTCA AGGAGCTTTG
    GTTTCCAAGA TTCCCTTCAG ACATCCTGCT CAGGTGCCTG CTCTCCTTGA CATTATAAGA
    CATCAGGCTG CTTACAACAC GTTGATTGGC AGTTGCGTTA AAAAGGCCTG CATTAAGGAA
    GCAGATACTC CTGGACTGCT GCAGTTTGAA GTGTGCCCAC TAACAGACTC AAGCTTTAGT
    GTGTCCTTTC AACACCCTGT AAATGAGTCA CTTGTTTGTG TGGTGATGGA GGTCATTGAT
    TCGATGCAGG TAGCCTGCAA ACTCTACAAG GGTTTATCTG ATGCTTTGAT CTGCACTGAT
    GACTTCATTA CTAAAGTAGT CCAAAGATGC ATGTCTATTC CGGTGACTAT GCGTGCCATA
    CGACGCAAGG CAGAGACCAT TCAGGCAGAC ACACCAGCGT TGTCCCTTAT AGCTGAAACA
    GTGGAAATTA TGGTGAAGAA GAACTTGCCC CCAACTGGCA GCCCAGGTTA CATGGGAATG
    GGTACAGGGC CAGATGGAAG TAACCCAATG GGTCTCCCTT TAAATAGTGG AAACAACAGT
    GTTGTGTGTG TAGGGGGGAA TGGATCTTTC CAAGGTCCAA TCACCTCAAT GTTCAACATG
    GGACGTACTG GTCAAGGAAG CTCGTGCATT GATACCATGG GACAGGTTGG AGGTCAGCAG
    CAAGCAATGC AGCAGCAACA TCAACAATCT CAGAGCCATG GGTCAGATGA CTTTAACAAA
    GTCACTCAGA ATCCAATTCT AACCAGCCTT TTGCAAATAA CAGGCAGTGT TGGGTCCAGT
    CCTACTCCCC AGCAACCTCA ACCCCACCAA ACACCTCCAA CAACCACCTC ACCAGCAAGC
    AACACCAAAA ACCACCCTAT GCTAATGAAC CTACTGAAGG ATAATCCATC ACAGGATTTT
    GCTGCATTGT ACAGCAGTAG CCCTCTAGAA AGACAGAACT CATCTGGGTC TCCACGGACT
    GACAACCAAG GCCAGCCTTG CTCTGGGACC AAGGGCAAAA AAAAGCGTCT GAGGGGCTCT
    GATAAAGGAG TAATGGGAGC AGCTGGGGGA ATGAGTATGA AACAATCTCA GCAGCAAGTG
    GTAGACATGA CACAACATCA TCACCTCACA AGCCACTCTG AAGATGATTT CCACCGAGAG
    CTTTTTTCTA TGGATGTAGA TGCCTCCCAA AACCCGATTT TTGATGTTAG TCTTCCAGGT
    GATGGACTTG ACACTCCACA CAGTATCACT CCAGCTCCAA GTCAGTGTGG AACACCACCT
    CCAGGTCCCG GAATGCAATA CCACCCACAA TGTCATGTCC AGCCTCAGAT ACAAGTCCAG
    TCACAATCAC AAGGATCCAT TCCTCGAATG GTCCGCCTCT CCAGCTCTGA CAGTATTGGA
    CCTGATATTA ATGACATTCT TTCTGATATT CCTGATCAAG CCAACAAAGG AAGCAATATC
    GGTCATGGAC AGCATCTTAT GACTAGTGAA GATGGAGGTT CCTTGGGAAC CCCAGTCCGG
    GACTCTTCCA GCTCTGGCCA GGGTAGTACA GTGTTTGAGG CAGACCTGTT TAATACAAAT
    AGTAATGAAA ATCCCTTTAC TGATGCAGCA GATTTGATTG CAGAAGCTGC TACTGCAACA
    ACACCCAATA GTGATACTTC CTCAACTAAC TTCTTCCCAG ACACAGACTT CAACCCAGAG
    CTTTTATCTG GGCAGGGAAG TTTTTCACAG ACCTACTTTG AAGACAGCTC TCCCAGCCCT
    GATCCAGATC TTGAATTGGT TAAAAGTTTC AGTGGAGGAA GTCAGCAGAA CACTCCTACA
    GGCACACCTC AGAATCCTGC CTCACATGGC CAAAATACTC CTGAGACTTC ATTAAAAGAC
    CCTTTTGAAA TTGGTATTTT TGGTGGAAAC GGTGGGGGAG GAAAACCATT AATGGCTCCA
    GCATCAGATA TTGCAGACTC GCACCATGCT CATACTGGTG GAGGTCAAAG TCCACTTATT
    GGAATTGTTG TTGCAGGTAA TGATTTTAAA AATGTAGATG CAAAGGCTAA GCAGCAGCAG
    ATGAGGACAA AAGAAGATCA TGTTGGAGGC AGTTCTGGAC AAGGAATGGT TAGTGCATGC
    TCATCTGAAA TGAAGCAGGT AAAGCGCAGT CGTACTCCTT CAAGTGAGGG AAAATCAAAA
    GACAAACCAG CTAAACGCAA GAAGCTGGAG CCAGATGCTA AGTCACCTTC ACATAGTGCA
    GGGGGTCGAC CTTACACTCC ACCTTGTGGA AGTGCCACTT CTGTAGGTTT AGTAAGTACT
    GGTGGATCTA AGTCCCCTGG GAGTGTAGGT AGGTCTCAGA CCCCACCAGG AGGTGCTACT
    CCACCAATCC CTAAAATCAC TATACAAATA CCCAGAGCTT TAACAGGAGG AAAAACATCA
    TCTCATGGAG GTTACACATC TAGTAGCTCA ACAAGTGGTG GTACTGGTAA TACAATTGGC
    ACAAGCAATA GCAAAAGCCA TCATTCCTCT TCATCTGGGA AGATAAAAAG TAGTAAACTT
    GAAAGTTCTG TTGGGCAGGG CAATATTGCA AAACAATCAA GTGCTGCTGG TACTGGAAGT
    GGTATACCTT CTCAATCTAA AGTGTCTTCC ATGGGCATGG GCACAGGCAA GCCATCTTCT
    TCTCCTATTA CGAAGCATGG TTTGTCAAGC TCGGGAGGTG GTGGCAGCAG TGGATCAGGA
    AGTGGAATTA AAAACAAACC TCAAGGTGGT AAGCCCCCTG GCTCACTCAT GAACCCCAAC
    ATCAAGCCCA ACATATCACC CTCGCACTCT CGTTCTGGAG GTTCTGACAA GCTGTCATCT
    CCCATGAAAC AAGGCCAAGT ACCTGGGACC CCACCCTCTT CTAAGGCTAA ATCCCCTATT
    GGCTCAGGCA GTGGATCAGG GGGCAATAAG TGCTCTTCGG GTGGTGGAGT TGGATCAAAA
    TCATTAAGCA GCAGTTCATC CAACAGCGCT TCTACATCCT GTGCTTCCTC CACTGGCTCC
    TCGTCATCTG GCTCTATGCC CTTTTCTTCA AATGGTCAGT CACAATATGG AAGCAGTGGT
    GGAAATTGCA GTGGTGGAGG GGGTGGTGGT GGCTGTGGAA ACAACCCAAA TGCAAAGGGT
    AAGTCCCCAA GCCGAAACAA AAAGCCATCT CTTACAGCTG TCATAGACAA ACTCAAGAGT
    GTGGGAAGTG GGGGTAATGG TGGGGAGGAA ATTGAAAGTT GTGGTGGTTC AAGTGGAGTA
    GGGCAGCAAA ATACTGTTCC TACATCCTCA AAGCATTCTA TGTCCTCCCA AGGAGGTGAG
    TTTTTGAGCA AACGAGACAA GATAGACAAA GATGGGAAAT CCAAGATGTC TGGATCAGGG
    GGGAATTCAA GTGACAAAAA GATGATTGAT GCCAAAAGTA ACACAATGAC TGGTACTGGT
    GTGGCAAAGA TTATTATCAG CAAGCCAGAA GGAGGCTCTC CAAGCATTAA ATCTAAAGTA
    ACACTTCAGA AACCTGGGGA AGGTTCTGGT GATGGTGGCA TTCGTTCCCA ACACTCAGGC
    CACAAAGCAT CACCTCTATT TAGTGGTTCT ACACCAAAAC ATGACCGCAG CTCCCCAAGC
    CACAGCCGTT CTCCTGGGTG TACACCTATG AATCCAGACA GTGAGAGTGA GTCAGGTAGC
    AGTTCAGTTG CTGAAAAGTC ACATCAGAAT AGTCCTAGCT CTGATGATGA CCAAACTATG
    AGATTACACC AACCAATTCA AGACTACATG AGCTCCATAT CTATTAGCCA GAGTGAAAAA
    CACAAAAAGC ATAAAAAAGA GAAAAAGAAG CAGAAAGATC GTGAAAGAGA TAAGGAGAGA
    GATAAGGAAA AAGAAAAGAA GAAGTCAATG TCTTGTGGGC CCTCACACAC AGTAAAGACA
    GATAATTGGT CTAGATCTCC TATATCTTCA GAAACATCAG TGTCATTGCT TAGCTCTGAG
    CGTTCATCAC GACCAAGTCC TGTGTACATG CATACAGAGG ATGATGATCT CATGGACTCT
    GCCCTAACAG GCAATCTAGA ACCTTTCAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54499
    Clone ID Related Accession (Same CDS sequence) XM_021480457.1 , XM_017358511.1
    Accession Version XM_017358511.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product mediator of RNA polymerase II transcription subunit 1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) and transcript sequence (GDQH01012616.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAAATTC GGAAGATGGC GGCGGTGCAA AGCAGCAGTC CGGTCGCAGA CTCGTCCCAG 
    GGAGGCACAG CCGCGGGCTC CGAGCGTCCC CGAGCCGAGG ATGGATCTGA GTCTGAGAAA
    CAGAGTCGTA TAATGACACT GCTAGAAAGG CTCTATTGCA AACACAGCTC ATCCAGGTCC
    TGGCAGGAGA CCAGCAAAGT GATACGGCAG GCAATGGAAA AGCGTGGAGC TGTTAATACA
    GGTGGACACC AGCTATTGCT CAACTGTTTA GAGACACTGC AAAGGGCATT AAAAGTTTCT
    ACACTGCCAT CAATGACAGA TCGCCTGGAG TCCATTGCTC GACAAAACAT GTTGGGATCT
    CACCTGAGTC CTTCACATAC TGAATGCTAT ATCACCTCTG ATATGTTCTA CGTTGAGGTG
    CATATGGATA AAAGTGGAAA GCTCATGGAT GTAAAAGTGG CCCACCATGG TGAAAATCCT
    GTGAGTTGTC CTGAGCTGAT ACAGCATTTA CGAGACAAGA ATTTTGATGA GTTTTCCAAA
    CACCTGAAGG GTCTGGTCAA CCTGTACAAT CTCCCTGGAG ACAACAAATT AAAGACAAAG
    ATGTACTTGG CACTTCAGTC ACTGGAGCTA GACCTCACAA AGATGATGCA TATGTTCAGG
    ATTGCCACTA ATGCTAACAC TGTGGAGACC ATACTTCACG GAAGTGTGGG TCTGTTGACG
    TCGAGGAGTG GTGGCCATTT AGTATCTCTG CAGTGTTATG TTTCTCCAAA TGACATCTTT
    GAGGAGGAAA AAGCAGCCCA TCTGAACACT GATGTCAATA TCCCCCATAC GTTAGGTGTG
    AGTGTGTCAG TGACTGTTGA AGGCACAACA TCAGTTTACA AGCTTCCCAT TGCTCCACTA
    ATCACAGGAT CCCATCCAGT TGACAACAAA GGAACTCCAT CATTTTCTTC CATAACAAAT
    TCAAACTGTG TAGATTTGCC AGCGTGTTTC TTCCTGAAGA TGAACTGCTT GATGCCATTT
    TCTTTATCAT ACATTAAAAA AATGGGCAGT ACTACAGGCA TTCCTGTCTT TGAAACTGCG
    CCCACCTTGT CCCCACTATA TGATCTCATC ATTAAGAGTC AACTCCGTGA GGAGGGAGGT
    GCCAGCCCCC CTTCGGCTCA ATCAATGCGT TTTTATGCAT CTTTACCAGG ACAGCAGCAC
    TGTTATTTCC TAAATGGTGA TGCTCCAGTA CAAGATGGAA GGTCTCTTCA AGGAGCTTTG
    GTTTCCAAGA TTCCCTTCAG ACATCCTGCT CAGGTGCCTG CTCTCCTTGA CATTATAAGA
    CATCAGGCTG CTTACAACAC GTTGATTGGC AGTTGCGTTA AAAAGGCCTG CATTAAGGAA
    GCAGATACTC CTGGACTGCT GCAGTTTGAA GTGTGCCCAC TAACAGACTC AAGCTTTAGT
    GTGTCCTTTC AACACCCTGT AAATGAGTCA CTTGTTTGTG TGGTGATGGA GGTCATTGAT
    TCGATGCAGG TAGCCTGCAA ACTCTACAAG GGTTTATCTG ATGCTTTGAT CTGCACTGAT
    GACTTCATTA CTAAAGTAGT CCAAAGATGC ATGTCTATTC CGGTGACTAT GCGTGCCATA
    CGACGCAAGG CAGAGACCAT TCAGGCAGAC ACACCAGCGT TGTCCCTTAT AGCTGAAACA
    GTGGAAATTA TGGTGAAGAA GAACTTGCCC CCAACTGGCA GCCCAGGTTA CATGGGAATG
    GGTACAGGGC CAGATGGAAG TAACCCAATG GGTCTCCCTT TAAATAGTGG AAACAACAGT
    GTTGTGTGTG TAGGGGGGAA TGGATCTTTC CAAGGTCCAA TCACCTCAAT GTTCAACATG
    GGACGTACTG GTCAAGGAAG CTCGTGCATT GATACCATGG GACAGGTTGG AGGTCAGCAG
    CAAGCAATGC AGCAGCAACA TCAACAATCT CAGAGCCATG GGTCAGATGA CTTTAACAAA
    GTCACTCAGA ATCCAATTCT AACCAGCCTT TTGCAAATAA CAGGCAGTGT TGGGTCCAGT
    CCTACTCCCC AGCAACCTCA ACCCCACCAA ACACCTCCAA CAACCACCTC ACCAGCAAGC
    AACACCAAAA ACCACCCTAT GCTAATGAAC CTACTGAAGG ATAATCCATC ACAGGATTTT
    GCTGCATTGT ACAGCAGTAG CCCTCTAGAA AGACAGAACT CATCTGGGTC TCCACGGACT
    GACAACCAAG GCCAGCCTTG CTCTGGGACC AAGGGCAAAA AAAAGCGTCT GAGGGGCTCT
    GATAAAGGAG TAATGGGAGC AGCTGGGGGA ATGAGTATGA AACAATCTCA GCAGCAAGTG
    GTAGACATGA CACAACATCA TCACCTCACA AGCCACTCTG AAGATGATTT CCACCGAGAG
    CTTTTTTCTA TGGATGTAGA TGCCTCCCAA AACCCGATTT TTGATGTTAG TCTTCCAGGT
    GATGGACTTG ACACTCCACA CAGTATCACT CCAGCTCCAA GTCAGTGTGG AACACCACCT
    CCAGGTCCCG GAATGCAATA CCACCCACAA TGTCATGTCC AGCCTCAGAT ACAAGTCCAG
    TCACAATCAC AAGGATCCAT TCCTCGAATG GTCCGCCTCT CCAGCTCTGA CAGTATTGGA
    CCTGATATTA ATGACATTCT TTCTGATATT CCTGATCAAG CCAACAAAGG AAGCAATATC
    GGTCATGGAC AGCATCTTAT GACTAGTGAA GATGGAGGTT CCTTGGGAAC CCCAGTCCGG
    GACTCTTCCA GCTCTGGCCA GGGTAGTACA GTGTTTGAGG CAGACCTGTT TAATACAAAT
    AGTAATGAAA ATCCCTTTAC TGATGCAGCA GATTTGATTG CAGAAGCTGC TACTGCAACA
    ACACCCAATA GTGATACTTC CTCAACTAAC TTCTTCCCAG ACACAGACTT CAACCCAGAG
    CTTTTATCTG GGCAGGGAAG TTTTTCACAG ACCTACTTTG AAGACAGCTC TCCCAGCCCT
    GATCCAGATC TTGAATTGGT TAAAAGTTTC AGTGGAGGAA GTCAGCAGAA CACTCCTACA
    GGCACACCTC AGAATCCTGC CTCACATGGC CAAAATACTC CTGAGACTTC ATTAAAAGAC
    CCTTTTGAAA TTGGTATTTT TGGTGGAAAC GGTGGGGGAG GAAAACCATT AATGGCTCCA
    GCATCAGATA TTGCAGACTC GCACCATGCT CATACTGGTG GAGGTCAAAG TCCACTTATT
    GGAATTGTTG TTGCAGGTAA TGATTTTAAA AATGTAGATG CAAAGGCTAA GCAGCAGCAG
    ATGAGGACAA AAGAAGATCA TGTTGGAGGC AGTTCTGGAC AAGGAATGGT TAGTGCATGC
    TCATCTGAAA TGAAGCAGGT AAAGCGCAGT CGTACTCCTT CAAGTGAGGG AAAATCAAAA
    GACAAACCAG CTAAACGCAA GAAGCTGGAG CCAGATGCTA AGTCACCTTC ACATAGTGCA
    GGGGGTCGAC CTTACACTCC ACCTTGTGGA AGTGCCACTT CTGTAGGTTT AGTAAGTACT
    GGTGGATCTA AGTCCCCTGG GAGTGTAGGT AGGTCTCAGA CCCCACCAGG AGGTGCTACT
    CCACCAATCC CTAAAATCAC TATACAAATA CCCAGAGCTT TAACAGGAGG AAAAACATCA
    TCTCATGGAG GTTACACATC TAGTAGCTCA ACAAGTGGTG GTACTGGTAA TACAATTGGC
    ACAAGCAATA GCAAAAGCCA TCATTCCTCT TCATCTGGGA AGATAAAAAG TAGTAAACTT
    GAAAGTTCTG TTGGGCAGGG CAATATTGCA AAACAATCAA GTGCTGCTGG TACTGGAAGT
    GGTATACCTT CTCAATCTAA AGTGTCTTCC ATGGGCATGG GCACAGGCAA GCCATCTTCT
    TCTCCTATTA CGAAGCATGG TTTGTCAAGC TCGGGAGGTG GTGGCAGCAG TGGATCAGGA
    AGTGGAATTA AAAACAAACC TCAAGGTGGT AAGCCCCCTG GCTCACTCAT GAACCCCAAC
    ATCAAGCCCA ACATATCACC CTCGCACTCT CGTTCTGGAG GTTCTGACAA GCTGTCATCT
    CCCATGAAAC AAGGCCAAGT ACCTGGGACC CCACCCTCTT CTAAGGCTAA ATCCCCTATT
    GGCTCAGGCA GTGGATCAGG GGGCAATAAG TGCTCTTCGG GTGGTGGAGT TGGATCAAAA
    TCATTAAGCA GCAGTTCATC CAACAGCGCT TCTACATCCT GTGCTTCCTC CACTGGCTCC
    TCGTCATCTG GCTCTATGCC CTTTTCTTCA AATGGTCAGT CACAATATGG AAGCAGTGGT
    GGAAATTGCA GTGGTGGAGG GGGTGGTGGT GGCTGTGGAA ACAACCCAAA TGCAAAGGGT
    AAGTCCCCAA GCCGAAACAA AAAGCCATCT CTTACAGCTG TCATAGACAA ACTCAAGAGT
    GTGGGAAGTG GGGGTAATGG TGGGGAGGAA ATTGAAAGTT GTGGTGGTTC AAGTGGAGTA
    GGGCAGCAAA ATACTGTTCC TACATCCTCA AAGCATTCTA TGTCCTCCCA AGGAGGTGAG
    TTTTTGAGCA AACGAGACAA GATAGACAAA GATGGGAAAT CCAAGATGTC TGGATCAGGG
    GGGAATTCAA GTGACAAAAA GATGATTGAT GCCAAAAGTA ACACAATGAC TGGTACTGGT
    GTGGCAAAGA TTATTATCAG CAAGCCAGAA GGAGGCTCTC CAAGCATTAA ATCTAAAGTA
    ACACTTCAGA AACCTGGGGA AGGTTCTGGT GATGGTGGCA TTCGTTCCCA ACACTCAGGC
    CACAAAGCAT CACCTCTATT TAGTGGTTCT ACACCAAAAC ATGACCGCAG CTCCCCAAGC
    CACAGCCGTT CTCCTGGGTG TACACCTATG AATCCAGACA GTGAGAGTGA GTCAGGTAGC
    AGTTCAGTTG CTGAAAAGTC ACATCAGAAT AGTCCTAGCT CTGATGATGA CCAAACTATG
    AGATTACACC AACCAATTCA AGACTACATG AGCTCCATAT CTATTAGCCA GAGTGAAAAA
    CACAAAAAGC ATAAAAAAGA GAAAAAGAAG CAGAAAGATC GTGAAAGAGA TAAGGAGAGA
    GATAAGGAAA AAGAAAAGAA GAAGTCAATG TCTTGTGGGC CCTCACACAC AGTAAAGACA
    GATAATTGGT CTAGATCTCC TATATCTTCA GAAACATCAG TGTCATTGCT TAGCTCTGAG
    CGTTCATCAC GACCAAGTCC TGTGTACATG CATACAGAGG ATGATGATCT CATGGACTCT
    GCCCTAACAG GCAATCTAGA ACCTTTCAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214000.1
    CDS189..5501
    Translation

    Target ORF information:

    RefSeq Version XM_017358511.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mediator complex subunit 1 (med1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358511.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCAAATTC GGAAGATGGC GGCGGTGCAA AGCAGCAGTC CGGTCGCAGA CTCGTCCCAG 
    GGAGGCACAG CCGCGGGCTC CGAGCGTCCC CGAGCCGAGG ATGGATCTGA GTCTGAGAAA
    CAGAGTCGTA TAATGACACT GCTAGAAAGG CTCTATTGCA AACACAGCTC ATCCAGGTCC
    TGGCAGGAGA CCAGCAAAGT GATACGGCAG GCAATGGAAA AGCGTGGAGC TGTTAATACA
    GGTGGACACC AGCTATTGCT CAACTGTTTA GAGACACTGC AAAGGGCATT AAAAGTTTCT
    ACACTGCCAT CAATGACAGA TCGCCTGGAG TCCATTGCTC GACAAAACAT GTTGGGATCT
    CACCTGAGTC CTTCACATAC TGAATGCTAT ATCACCTCTG ATATGTTCTA CGTTGAGGTG
    CATATGGATA AAAGTGGAAA GCTCATGGAT GTAAAAGTGG CCCACCATGG TGAAAATCCT
    GTGAGTTGTC CTGAGCTGAT ACAGCATTTA CGAGACAAGA ATTTTGATGA GTTTTCCAAA
    CACCTGAAGG GTCTGGTCAA CCTGTACAAT CTCCCTGGAG ACAACAAATT AAAGACAAAG
    ATGTACTTGG CACTTCAGTC ACTGGAGCTA GACCTCACAA AGATGATGCA TATGTTCAGG
    ATTGCCACTA ATGCTAACAC TGTGGAGACC ATACTTCACG GAAGTGTGGG TCTGTTGACG
    TCGAGGAGTG GTGGCCATTT AGTATCTCTG CAGTGTTATG TTTCTCCAAA TGACATCTTT
    GAGGAGGAAA AAGCAGCCCA TCTGAACACT GATGTCAATA TCCCCCATAC GTTAGGTGTG
    AGTGTGTCAG TGACTGTTGA AGGCACAACA TCAGTTTACA AGCTTCCCAT TGCTCCACTA
    ATCACAGGAT CCCATCCAGT TGACAACAAA GGAACTCCAT CATTTTCTTC CATAACAAAT
    TCAAACTGTG TAGATTTGCC AGCGTGTTTC TTCCTGAAGA TGAACTGCTT GATGCCATTT
    TCTTTATCAT ACATTAAAAA AATGGGCAGT ACTACAGGCA TTCCTGTCTT TGAAACTGCG
    CCCACCTTGT CCCCACTATA TGATCTCATC ATTAAGAGTC AACTCCGTGA GGAGGGAGGT
    GCCAGCCCCC CTTCGGCTCA ATCAATGCGT TTTTATGCAT CTTTACCAGG ACAGCAGCAC
    TGTTATTTCC TAAATGGTGA TGCTCCAGTA CAAGATGGAA GGTCTCTTCA AGGAGCTTTG
    GTTTCCAAGA TTCCCTTCAG ACATCCTGCT CAGGTGCCTG CTCTCCTTGA CATTATAAGA
    CATCAGGCTG CTTACAACAC GTTGATTGGC AGTTGCGTTA AAAAGGCCTG CATTAAGGAA
    GCAGATACTC CTGGACTGCT GCAGTTTGAA GTGTGCCCAC TAACAGACTC AAGCTTTAGT
    GTGTCCTTTC AACACCCTGT AAATGAGTCA CTTGTTTGTG TGGTGATGGA GGTCATTGAT
    TCGATGCAGG TAGCCTGCAA ACTCTACAAG GGTTTATCTG ATGCTTTGAT CTGCACTGAT
    GACTTCATTA CTAAAGTAGT CCAAAGATGC ATGTCTATTC CGGTGACTAT GCGTGCCATA
    CGACGCAAGG CAGAGACCAT TCAGGCAGAC ACACCAGCGT TGTCCCTTAT AGCTGAAACA
    GTGGAAATTA TGGTGAAGAA GAACTTGCCC CCAACTGGCA GCCCAGGTTA CATGGGAATG
    GGTACAGGGC CAGATGGAAG TAACCCAATG GGTCTCCCTT TAAATAGTGG AAACAACAGT
    GTTGTGTGTG TAGGGGGGAA TGGATCTTTC CAAGGTCCAA TCACCTCAAT GTTCAACATG
    GGACGTACTG GTCAAGGAAG CTCGTGCATT GATACCATGG GACAGGTTGG AGGTCAGCAG
    CAAGCAATGC AGCAGCAACA TCAACAATCT CAGAGCCATG GGTCAGATGA CTTTAACAAA
    GTCACTCAGA ATCCAATTCT AACCAGCCTT TTGCAAATAA CAGGCAGTGT TGGGTCCAGT
    CCTACTCCCC AGCAACCTCA ACCCCACCAA ACACCTCCAA CAACCACCTC ACCAGCAAGC
    AACACCAAAA ACCACCCTAT GCTAATGAAC CTACTGAAGG ATAATCCATC ACAGGATTTT
    GCTGCATTGT ACAGCAGTAG CCCTCTAGAA AGACAGAACT CATCTGGGTC TCCACGGACT
    GACAACCAAG GCCAGCCTTG CTCTGGGACC AAGGGCAAAA AAAAGCGTCT GAGGGGCTCT
    GATAAAGGAG TAATGGGAGC AGCTGGGGGA ATGAGTATGA AACAATCTCA GCAGCAAGTG
    GTAGACATGA CACAACATCA TCACCTCACA AGCCACTCTG AAGATGATTT CCACCGAGAG
    CTTTTTTCTA TGGATGTAGA TGCCTCCCAA AACCCGATTT TTGATGTTAG TCTTCCAGGT
    GATGGACTTG ACACTCCACA CAGTATCACT CCAGCTCCAA GTCAGTGTGG AACACCACCT
    CCAGGTCCCG GAATGCAATA CCACCCACAA TGTCATGTCC AGCCTCAGAT ACAAGTCCAG
    TCACAATCAC AAGGATCCAT TCCTCGAATG GTCCGCCTCT CCAGCTCTGA CAGTATTGGA
    CCTGATATTA ATGACATTCT TTCTGATATT CCTGATCAAG CCAACAAAGG AAGCAATATC
    GGTCATGGAC AGCATCTTAT GACTAGTGAA GATGGAGGTT CCTTGGGAAC CCCAGTCCGG
    GACTCTTCCA GCTCTGGCCA GGGTAGTACA GTGTTTGAGG CAGACCTGTT TAATACAAAT
    AGTAATGAAA ATCCCTTTAC TGATGCAGCA GATTTGATTG CAGAAGCTGC TACTGCAACA
    ACACCCAATA GTGATACTTC CTCAACTAAC TTCTTCCCAG ACACAGACTT CAACCCAGAG
    CTTTTATCTG GGCAGGGAAG TTTTTCACAG ACCTACTTTG AAGACAGCTC TCCCAGCCCT
    GATCCAGATC TTGAATTGGT TAAAAGTTTC AGTGGAGGAA GTCAGCAGAA CACTCCTACA
    GGCACACCTC AGAATCCTGC CTCACATGGC CAAAATACTC CTGAGACTTC ATTAAAAGAC
    CCTTTTGAAA TTGGTATTTT TGGTGGAAAC GGTGGGGGAG GAAAACCATT AATGGCTCCA
    GCATCAGATA TTGCAGACTC GCACCATGCT CATACTGGTG GAGGTCAAAG TCCACTTATT
    GGAATTGTTG TTGCAGGTAA TGATTTTAAA AATGTAGATG CAAAGGCTAA GCAGCAGCAG
    ATGAGGACAA AAGAAGATCA TGTTGGAGGC AGTTCTGGAC AAGGAATGGT TAGTGCATGC
    TCATCTGAAA TGAAGCAGGT AAAGCGCAGT CGTACTCCTT CAAGTGAGGG AAAATCAAAA
    GACAAACCAG CTAAACGCAA GAAGCTGGAG CCAGATGCTA AGTCACCTTC ACATAGTGCA
    GGGGGTCGAC CTTACACTCC ACCTTGTGGA AGTGCCACTT CTGTAGGTTT AGTAAGTACT
    GGTGGATCTA AGTCCCCTGG GAGTGTAGGT AGGTCTCAGA CCCCACCAGG AGGTGCTACT
    CCACCAATCC CTAAAATCAC TATACAAATA CCCAGAGCTT TAACAGGAGG AAAAACATCA
    TCTCATGGAG GTTACACATC TAGTAGCTCA ACAAGTGGTG GTACTGGTAA TACAATTGGC
    ACAAGCAATA GCAAAAGCCA TCATTCCTCT TCATCTGGGA AGATAAAAAG TAGTAAACTT
    GAAAGTTCTG TTGGGCAGGG CAATATTGCA AAACAATCAA GTGCTGCTGG TACTGGAAGT
    GGTATACCTT CTCAATCTAA AGTGTCTTCC ATGGGCATGG GCACAGGCAA GCCATCTTCT
    TCTCCTATTA CGAAGCATGG TTTGTCAAGC TCGGGAGGTG GTGGCAGCAG TGGATCAGGA
    AGTGGAATTA AAAACAAACC TCAAGGTGGT AAGCCCCCTG GCTCACTCAT GAACCCCAAC
    ATCAAGCCCA ACATATCACC CTCGCACTCT CGTTCTGGAG GTTCTGACAA GCTGTCATCT
    CCCATGAAAC AAGGCCAAGT ACCTGGGACC CCACCCTCTT CTAAGGCTAA ATCCCCTATT
    GGCTCAGGCA GTGGATCAGG GGGCAATAAG TGCTCTTCGG GTGGTGGAGT TGGATCAAAA
    TCATTAAGCA GCAGTTCATC CAACAGCGCT TCTACATCCT GTGCTTCCTC CACTGGCTCC
    TCGTCATCTG GCTCTATGCC CTTTTCTTCA AATGGTCAGT CACAATATGG AAGCAGTGGT
    GGAAATTGCA GTGGTGGAGG GGGTGGTGGT GGCTGTGGAA ACAACCCAAA TGCAAAGGGT
    AAGTCCCCAA GCCGAAACAA AAAGCCATCT CTTACAGCTG TCATAGACAA ACTCAAGAGT
    GTGGGAAGTG GGGGTAATGG TGGGGAGGAA ATTGAAAGTT GTGGTGGTTC AAGTGGAGTA
    GGGCAGCAAA ATACTGTTCC TACATCCTCA AAGCATTCTA TGTCCTCCCA AGGAGGTGAG
    TTTTTGAGCA AACGAGACAA GATAGACAAA GATGGGAAAT CCAAGATGTC TGGATCAGGG
    GGGAATTCAA GTGACAAAAA GATGATTGAT GCCAAAAGTA ACACAATGAC TGGTACTGGT
    GTGGCAAAGA TTATTATCAG CAAGCCAGAA GGAGGCTCTC CAAGCATTAA ATCTAAAGTA
    ACACTTCAGA AACCTGGGGA AGGTTCTGGT GATGGTGGCA TTCGTTCCCA ACACTCAGGC
    CACAAAGCAT CACCTCTATT TAGTGGTTCT ACACCAAAAC ATGACCGCAG CTCCCCAAGC
    CACAGCCGTT CTCCTGGGTG TACACCTATG AATCCAGACA GTGAGAGTGA GTCAGGTAGC
    AGTTCAGTTG CTGAAAAGTC ACATCAGAAT AGTCCTAGCT CTGATGATGA CCAAACTATG
    AGATTACACC AACCAATTCA AGACTACATG AGCTCCATAT CTATTAGCCA GAGTGAAAAA
    CACAAAAAGC ATAAAAAAGA GAAAAAGAAG CAGAAAGATC GTGAAAGAGA TAAGGAGAGA
    GATAAGGAAA AAGAAAAGAA GAAGTCAATG TCTTGTGGGC CCTCACACAC AGTAAAGACA
    GATAATTGGT CTAGATCTCC TATATCTTCA GAAACATCAG TGTCATTGCT TAGCTCTGAG
    CGTTCATCAC GACCAAGTCC TGTGTACATG CATACAGAGG ATGATGATCT CATGGACTCT
    GCCCTAACAG GCAATCTAGA ACCTTTCAAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.