RASEF cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASEF
Entrez Gene ID	570731
Full Name	RAS and EF-hand domain containing
Synonyms	si:ch73-275j21.1
General protein information	Preferred Names RAS and EF-hand domain containing Names ras and EF-hand domain-containing protein
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_694243.8	XP_699335.4	ras and EF-hand domain-containing protein
XM_017357544.1	XP_017213033.1	ras and EF-hand domain-containing protein
XM_694243.7	XP_699335.4	ras and EF-hand domain-containing protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RASEF	NP_001098832.1
Danio rerio (zebrafish)	rasef	XP_699335.4
Caenorhabditis elegans (roundworm)	tag-312	NP_508523.1
Pan troglodytes (chimpanzee)	RASEF	XP_520093.3
Homo sapiens (human)	RASEF	NP_689786.2
Canis lupus familiaris (dog)	RASEF	XP_541269.2
Mus musculus (house mouse)	Rasef	NP_001017427.1
Rattus norvegicus (Norway rat)	Rasef	NP_001264263.1
	RASEF	XP_001104352.1
Gallus gallus (chicken)	RASEF	XP_003643075.2
Xenopus tropicalis (tropical clawed frog)	MGC147600	NP_001072877.1

Gene Ontology

The following RASEF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASEF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa14655	XM_017357544.1 Latest version!	Danio rerio RAS and EF-hand domain containing (rasef), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa14655	XM_694243.8 Latest version!	Danio rerio RAS and EF-hand domain containing (rasef), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa14655	XM_694243.7	Danio rerio RAS and EF-hand domain containing (rasef), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASEF ( XM_694243.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_699335.4 Danio rerio RAS and EF-hand domain containing (rasef), mRNA.

Price & Availability↑

CloneID	ODa14655
Clone ID Related Accession (Same CDS sequence)	XM_694243.8 , XM_017357544.1 , XM_694243.7
Accession Version	XM_694243.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras and EF-hand domain-containing protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_694243.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG AGGCGCAGAT GCAGCGACTG CGCGAGCTCT TCCACGCCTG TGATGTGGAT 
CATTCAGGAC GGATCGAGAA ATGGGAGTTT GAGAAAATAT GCTCGGAGCT TCACGTCCAG 
TCCAGCGAGA TCGATTCGCT TTTCTCCAGA CTTGACACCG ACGAAGACGG GACGATAAAC 
CTGGAGGAGT TCATGGAGGG CTTTCAGGAG TCGCACCTGC TGAAGGAGCC GGAGATGAGC 
GGAGAGCCGG CCGGGGAAAC TTCCTCTGCC GCCTGGGAAG ACTTTAAATC GCGCCTTGGC 
GATCAGTTCA AATTCATCCC CAGGATTGAC CAGGTAGCAA CTCTGTACCA GAACATCCGT 
TTGACGGAGC CCAGAATGGT CCCTCAGTAT GAAAGGGTTC TTGTCAGTTT CATCAAGGAG 
ATCCGGATAC TGAACACAGA AATGGAGCAT CTGGCGCTGG CTGTCAAGAG AGCTCAGGAT 
CAGATGTCTA TGCAGCTGAG TGAGATGGAG GAAGAGATGG ATCACCGAAT CCACACCGCA 
GAGAGAAACA CACGTCTGCA GGAGTCTAAA AAAGCTGAGG CGACTCTGAG CAGCATGAAA 
CACCAGTATG ACTCTCAGAT TTGTGAACTC CAGCAGAAGA TTCAGACTCT GCAGATGATG 
GAGAACCAGA ACAGGAGCGT GAGCCTGAAA GAGGAAACAT CGGCACTTAA ACGGCAGAAC 
AACGAGCTCA TGCTGCAAAA CCAGAAGCTG AAGCAGGAGC TGTTGGAGTC CCACACTAAT 
GTTGCGTTCT TGCAAAGCGA GCTGGACTCT CTGAAGAGTG ATCTGACAGA CCAGAGCATC 
AACTGTGAAC GAGACGAGGC TCTTATGAAG TGCTTCTCTG AGGAGAGAGA TAACCTGGAG 
AGACAGATCG AGATGCTTCA GGTAGCAAAC AGAAAGCTGC ACGACAGTAA TGACAGTCTG 
CGTACGGCGC TGGAGAGCAG TCAGAGCAAG AGCAAGACTC AGCGTGTTAG CGGACTCTCA 
CCTGGAGTTT ACGTGAACAC CAGAAAGAAT GTCTGCCACT CTTATTTTGA TCGGTGCTGC 
AACACGTGTG TGGATGAGGA AGACTACGAT CAATTTCGGC TGATGGCGGG AATGCGCAGA 
CAGAGCTGTG ATTCTCTGGC TCTGGCTTTG TGTGACCCGA TGCGCAGACG CAGCTGCGAG 
GAGGACAGTC TCCCAGACAG CGGCATGTCT ACCCTGCGCT CCAATATCGG ATATGATTAT 
GAGCATGAGA GAACATCCAG TCCTGTGGAG GCAGAAGACA GCAACACCAC AGTGTCTGGA 
GACACGTCTG ATACTGAGGT TGTGGAAATG CGAGAAGAGG CTGCGTCAGG GTCGGACAGC 
GAATCCGTTC AGAGCTGGAT GCCATCAAAG CCTGTTCAGA CGGATGACGT TTCTGCTTCA 
GGGAAGAAAT GCCTTTCTGC TATTTTCACA GAGAGAGAGC AGGATGAGCT GGCCGTGTCT 
CAGTCTCCAT CTGAGAAGGC TTACAGGATC GTGCTGGCAG GAGACGCTGC AGTGGGAAAG 
TCCAGCTTTC TTCTCCGTCT CTGTAAGAAT GAGTTCAAAG GCAACACCAG CGCCACACTG 
GGTGTTGATT TCCAGATGAA AACTCTGGTT GTGGATGGCG TTCCAACAGT GCTGCAGTTA 
TGGGACACGG CTGGACAGGA AAGGTTTCGG AGCATTGCTA AGTCATACTT CAGGAGAGCG 
GATGGAGTTC TTCTGCTGTA CGACGTCACC TGTGAGAAGA GCTTTCTGAA TGTGCGAGAA 
TGGGTCGACA TCATCGAGGA TGTTTCTCAG GATGACATTC CCATCATGCT AGTGGGCAAT 
AAGACAGATC TGAGGAAGGA GGCTCTTCAG GACGGAGTCA CCTGCATTCC AACCAGCTAT 
GGAGAGAAAC TGGCCATGAC ATACAGTGCC TTATTCTGTG AGACGAGTGC AAAAGATGGA 
TCCAACATCA TTGAAGCTGT TCTTCATTTA GCCCGAGAGG TGACCAAACA CGCCGACGAT 
TACAAAGAGC CTGTGTCAGT GGCCAAACTG TCTGGAAATC ATACCAAGAA GATGTCCAGT 
CCAAACTGCT GCATGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_699335.4
CDS	119..2239
Translation	MGSEAQMQRLRELFHACDVDHSGRIEKWEFEKICSELHVQSSEIDSLFSRLDTDEDGTINLEEFMEGFQESHLLKEPEMSGEPAGETSSAAWEDFKSRLGDQFKFIPRIDQVATLYQNIRLTEPRMVPQYERVLVSFIKEIRILNTEMEHLALAVKRAQDQMSMQLSEMEEEMDHRIHTAERNTRLQESKKAEATLSSMKHQYDSQICELQQKIQTLQMMENQNRSVSLKEETSALKRQNNELMLQNQKLKQELLESHTNVAFLQSELDSLKSDLTDQSINCERDEALMKCFSEERDNLERQIEMLQVANRKLHDSNDSLRTALESSQSKSKTQRVSGLSPGVYVNTRKNVCHSYFDRCCNTCVDEEDYDQFRLMAGMRRQSCDSLALALCDPMRRRSCEEDSLPDSGMSTLRSNIGYDYEHERTSSPVEAEDSNTTVSGDTSDTEVVEMREEAASGSDSESVQSWMPSKPVQTDDVSASGKKCLSAIFTEREQDELAVSQSPSEKAYRIVLAGDAAVGKSSFLLRLCKNEFKGNTSATLGVDFQMKTLVVDGVPTVLQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWVDIIEDVSQDDIPIMLVGNKTDLRKEALQDGVTCIPTSYGEKLAMTYSALFCETSAKDGSNIIEAVLHLAREVTKHADDYKEPVSVAKLSGNHTKKMSSPNCCMG

Target ORF information:

RefSeq Version	XM_694243.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RAS and EF-hand domain containing (rasef), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_694243.8

ORF Insert Sequence:

ATGGGCTCTG AGGCGCAGAT GCAGCGACTG CGCGAGCTCT TCCACGCCTG TGATGTGGAT 
CATTCAGGAC GGATCGAGAA ATGGGAGTTT GAGAAAATAT GCTCGGAGCT TCACGTCCAG 
TCCAGCGAGA TCGATTCGCT TTTCTCCAGA CTTGACACCG ACGAAGACGG GACGATAAAC 
CTGGAGGAGT TCATGGAGGG CTTTCAGGAG TCGCACCTGC TGAAGGAGCC GGAGATGAGC 
GGAGAGCCGG CCGGGGAAAC TTCCTCTGCC GCCTGGGAAG ACTTTAAATC GCGCCTTGGC 
GATCAGTTCA AATTCATCCC CAGGATTGAC CAGGTAGCAA CTCTGTACCA GAACATCCGT 
TTGACGGAGC CCAGAATGGT CCCTCAGTAT GAAAGGGTTC TTGTCAGTTT CATCAAGGAG 
ATCCGGATAC TGAACACAGA AATGGAGCAT CTGGCGCTGG CTGTCAAGAG AGCTCAGGAT 
CAGATGTCTA TGCAGCTGAG TGAGATGGAG GAAGAGATGG ATCACCGAAT CCACACCGCA 
GAGAGAAACA CACGTCTGCA GGAGTCTAAA AAAGCTGAGG CGACTCTGAG CAGCATGAAA 
CACCAGTATG ACTCTCAGAT TTGTGAACTC CAGCAGAAGA TTCAGACTCT GCAGATGATG 
GAGAACCAGA ACAGGAGCGT GAGCCTGAAA GAGGAAACAT CGGCACTTAA ACGGCAGAAC 
AACGAGCTCA TGCTGCAAAA CCAGAAGCTG AAGCAGGAGC TGTTGGAGTC CCACACTAAT 
GTTGCGTTCT TGCAAAGCGA GCTGGACTCT CTGAAGAGTG ATCTGACAGA CCAGAGCATC 
AACTGTGAAC GAGACGAGGC TCTTATGAAG TGCTTCTCTG AGGAGAGAGA TAACCTGGAG 
AGACAGATCG AGATGCTTCA GGTAGCAAAC AGAAAGCTGC ACGACAGTAA TGACAGTCTG 
CGTACGGCGC TGGAGAGCAG TCAGAGCAAG AGCAAGACTC AGCGTGTTAG CGGACTCTCA 
CCTGGAGTTT ACGTGAACAC CAGAAAGAAT GTCTGCCACT CTTATTTTGA TCGGTGCTGC 
AACACGTGTG TGGATGAGGA AGACTACGAT CAATTTCGGC TGATGGCGGG AATGCGCAGA 
CAGAGCTGTG ATTCTCTGGC TCTGGCTTTG TGTGACCCGA TGCGCAGACG CAGCTGCGAG 
GAGGACAGTC TCCCAGACAG CGGCATGTCT ACCCTGCGCT CCAATATCGG ATATGATTAT 
GAGCATGAGA GAACATCCAG TCCTGTGGAG GCAGAAGACA GCAACACCAC AGTGTCTGGA 
GACACGTCTG ATACTGAGGT TGTGGAAATG CGAGAAGAGG CTGCGTCAGG GTCGGACAGC 
GAATCCGTTC AGAGCTGGAT GCCATCAAAG CCTGTTCAGA CGGATGACGT TTCTGCTTCA 
GGGAAGAAAT GCCTTTCTGC TATTTTCACA GAGAGAGAGC AGGATGAGCT GGCCGTGTCT 
CAGTCTCCAT CTGAGAAGGC TTACAGGATC GTGCTGGCAG GAGACGCTGC AGTGGGAAAG 
TCCAGCTTTC TTCTCCGTCT CTGTAAGAAT GAGTTCAAAG GCAACACCAG CGCCACACTG 
GGTGTTGATT TCCAGATGAA AACTCTGGTT GTGGATGGCG TTCCAACAGT GCTGCAGTTA 
TGGGACACGG CTGGACAGGA AAGGTTTCGG AGCATTGCTA AGTCATACTT CAGGAGAGCG 
GATGGAGTTC TTCTGCTGTA CGACGTCACC TGTGAGAAGA GCTTTCTGAA TGTGCGAGAA 
TGGGTCGACA TCATCGAGGA TGTTTCTCAG GATGACATTC CCATCATGCT AGTGGGCAAT 
AAGACAGATC TGAGGAAGGA GGCTCTTCAG GACGGAGTCA CCTGCATTCC AACCAGCTAT 
GGAGAGAAAC TGGCCATGAC ATACAGTGCC TTATTCTGTG AGACGAGTGC AAAAGATGGA 
TCCAACATCA TTGAAGCTGT TCTTCATTTA GCCCGAGAGG TGACCAAACA CGCCGACGAT 
TACAAAGAGC CTGTGTCAGT GGCCAAACTG TCTGGAAATC ATACCAAGAA GATGTCCAGT 
CCAAACTGCT GCATGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASEF ( XM_017357544.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213033.1 Danio rerio RAS and EF-hand domain containing (rasef), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa14655
Clone ID Related Accession (Same CDS sequence)	XM_694243.8 , XM_017357544.1 , XM_694243.7
Accession Version	XM_017357544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras and EF-hand domain-containing protein
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG AGGCGCAGAT GCAGCGACTG CGCGAGCTCT TCCACGCCTG TGATGTGGAT 
CATTCAGGAC GGATCGAGAA ATGGGAGTTT GAGAAAATAT GCTCGGAGCT TCACGTCCAG 
TCCAGCGAGA TCGATTCGCT TTTCTCCAGA CTTGACACCG ACGAAGACGG GACGATAAAC 
CTGGAGGAGT TCATGGAGGG CTTTCAGGAG TCGCACCTGC TGAAGGAGCC GGAGATGAGC 
GGAGAGCCGG CCGGGGAAAC TTCCTCTGCC GCCTGGGAAG ACTTTAAATC GCGCCTTGGC 
GATCAGTTCA AATTCATCCC CAGGATTGAC CAGGTAGCAA CTCTGTACCA GAACATCCGT 
TTGACGGAGC CCAGAATGGT CCCTCAGTAT GAAAGGGTTC TTGTCAGTTT CATCAAGGAG 
ATCCGGATAC TGAACACAGA AATGGAGCAT CTGGCGCTGG CTGTCAAGAG AGCTCAGGAT 
CAGATGTCTA TGCAGCTGAG TGAGATGGAG GAAGAGATGG ATCACCGAAT CCACACCGCA 
GAGAGAAACA CACGTCTGCA GGAGTCTAAA AAAGCTGAGG CGACTCTGAG CAGCATGAAA 
CACCAGTATG ACTCTCAGAT TTGTGAACTC CAGCAGAAGA TTCAGACTCT GCAGATGATG 
GAGAACCAGA ACAGGAGCGT GAGCCTGAAA GAGGAAACAT CGGCACTTAA ACGGCAGAAC 
AACGAGCTCA TGCTGCAAAA CCAGAAGCTG AAGCAGGAGC TGTTGGAGTC CCACACTAAT 
GTTGCGTTCT TGCAAAGCGA GCTGGACTCT CTGAAGAGTG ATCTGACAGA CCAGAGCATC 
AACTGTGAAC GAGACGAGGC TCTTATGAAG TGCTTCTCTG AGGAGAGAGA TAACCTGGAG 
AGACAGATCG AGATGCTTCA GGTAGCAAAC AGAAAGCTGC ACGACAGTAA TGACAGTCTG 
CGTACGGCGC TGGAGAGCAG TCAGAGCAAG AGCAAGACTC AGCGTGTTAG CGGACTCTCA 
CCTGGAGTTT ACGTGAACAC CAGAAAGAAT GTCTGCCACT CTTATTTTGA TCGGTGCTGC 
AACACGTGTG TGGATGAGGA AGACTACGAT CAATTTCGGC TGATGGCGGG AATGCGCAGA 
CAGAGCTGTG ATTCTCTGGC TCTGGCTTTG TGTGACCCGA TGCGCAGACG CAGCTGCGAG 
GAGGACAGTC TCCCAGACAG CGGCATGTCT ACCCTGCGCT CCAATATCGG ATATGATTAT 
GAGCATGAGA GAACATCCAG TCCTGTGGAG GCAGAAGACA GCAACACCAC AGTGTCTGGA 
GACACGTCTG ATACTGAGGT TGTGGAAATG CGAGAAGAGG CTGCGTCAGG GTCGGACAGC 
GAATCCGTTC AGAGCTGGAT GCCATCAAAG CCTGTTCAGA CGGATGACGT TTCTGCTTCA 
GGGAAGAAAT GCCTTTCTGC TATTTTCACA GAGAGAGAGC AGGATGAGCT GGCCGTGTCT 
CAGTCTCCAT CTGAGAAGGC TTACAGGATC GTGCTGGCAG GAGACGCTGC AGTGGGAAAG 
TCCAGCTTTC TTCTCCGTCT CTGTAAGAAT GAGTTCAAAG GCAACACCAG CGCCACACTG 
GGTGTTGATT TCCAGATGAA AACTCTGGTT GTGGATGGCG TTCCAACAGT GCTGCAGTTA 
TGGGACACGG CTGGACAGGA AAGGTTTCGG AGCATTGCTA AGTCATACTT CAGGAGAGCG 
GATGGAGTTC TTCTGCTGTA CGACGTCACC TGTGAGAAGA GCTTTCTGAA TGTGCGAGAA 
TGGGTCGACA TCATCGAGGA TGTTTCTCAG GATGACATTC CCATCATGCT AGTGGGCAAT 
AAGACAGATC TGAGGAAGGA GGCTCTTCAG GACGGAGTCA CCTGCATTCC AACCAGCTAT 
GGAGAGAAAC TGGCCATGAC ATACAGTGCC TTATTCTGTG AGACGAGTGC AAAAGATGGA 
TCCAACATCA TTGAAGCTGT TCTTCATTTA GCCCGAGAGG TGACCAAACA CGCCGACGAT 
TACAAAGAGC CTGTGTCAGT GGCCAAACTG TCTGGAAATC ATACCAAGAA GATGTCCAGT 
CCAAACTGCT GCATGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213033.1
CDS	81..2201
Translation	MGSEAQMQRLRELFHACDVDHSGRIEKWEFEKICSELHVQSSEIDSLFSRLDTDEDGTINLEEFMEGFQESHLLKEPEMSGEPAGETSSAAWEDFKSRLGDQFKFIPRIDQVATLYQNIRLTEPRMVPQYERVLVSFIKEIRILNTEMEHLALAVKRAQDQMSMQLSEMEEEMDHRIHTAERNTRLQESKKAEATLSSMKHQYDSQICELQQKIQTLQMMENQNRSVSLKEETSALKRQNNELMLQNQKLKQELLESHTNVAFLQSELDSLKSDLTDQSINCERDEALMKCFSEERDNLERQIEMLQVANRKLHDSNDSLRTALESSQSKSKTQRVSGLSPGVYVNTRKNVCHSYFDRCCNTCVDEEDYDQFRLMAGMRRQSCDSLALALCDPMRRRSCEEDSLPDSGMSTLRSNIGYDYEHERTSSPVEAEDSNTTVSGDTSDTEVVEMREEAASGSDSESVQSWMPSKPVQTDDVSASGKKCLSAIFTEREQDELAVSQSPSEKAYRIVLAGDAAVGKSSFLLRLCKNEFKGNTSATLGVDFQMKTLVVDGVPTVLQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWVDIIEDVSQDDIPIMLVGNKTDLRKEALQDGVTCIPTSYGEKLAMTYSALFCETSAKDGSNIIEAVLHLAREVTKHADDYKEPVSVAKLSGNHTKKMSSPNCCMG

Target ORF information:

RefSeq Version	XM_017357544.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RAS and EF-hand domain containing (rasef), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357544.1

ORF Insert Sequence:

ATGGGCTCTG AGGCGCAGAT GCAGCGACTG CGCGAGCTCT TCCACGCCTG TGATGTGGAT 
CATTCAGGAC GGATCGAGAA ATGGGAGTTT GAGAAAATAT GCTCGGAGCT TCACGTCCAG 
TCCAGCGAGA TCGATTCGCT TTTCTCCAGA CTTGACACCG ACGAAGACGG GACGATAAAC 
CTGGAGGAGT TCATGGAGGG CTTTCAGGAG TCGCACCTGC TGAAGGAGCC GGAGATGAGC 
GGAGAGCCGG CCGGGGAAAC TTCCTCTGCC GCCTGGGAAG ACTTTAAATC GCGCCTTGGC 
GATCAGTTCA AATTCATCCC CAGGATTGAC CAGGTAGCAA CTCTGTACCA GAACATCCGT 
TTGACGGAGC CCAGAATGGT CCCTCAGTAT GAAAGGGTTC TTGTCAGTTT CATCAAGGAG 
ATCCGGATAC TGAACACAGA AATGGAGCAT CTGGCGCTGG CTGTCAAGAG AGCTCAGGAT 
CAGATGTCTA TGCAGCTGAG TGAGATGGAG GAAGAGATGG ATCACCGAAT CCACACCGCA 
GAGAGAAACA CACGTCTGCA GGAGTCTAAA AAAGCTGAGG CGACTCTGAG CAGCATGAAA 
CACCAGTATG ACTCTCAGAT TTGTGAACTC CAGCAGAAGA TTCAGACTCT GCAGATGATG 
GAGAACCAGA ACAGGAGCGT GAGCCTGAAA GAGGAAACAT CGGCACTTAA ACGGCAGAAC 
AACGAGCTCA TGCTGCAAAA CCAGAAGCTG AAGCAGGAGC TGTTGGAGTC CCACACTAAT 
GTTGCGTTCT TGCAAAGCGA GCTGGACTCT CTGAAGAGTG ATCTGACAGA CCAGAGCATC 
AACTGTGAAC GAGACGAGGC TCTTATGAAG TGCTTCTCTG AGGAGAGAGA TAACCTGGAG 
AGACAGATCG AGATGCTTCA GGTAGCAAAC AGAAAGCTGC ACGACAGTAA TGACAGTCTG 
CGTACGGCGC TGGAGAGCAG TCAGAGCAAG AGCAAGACTC AGCGTGTTAG CGGACTCTCA 
CCTGGAGTTT ACGTGAACAC CAGAAAGAAT GTCTGCCACT CTTATTTTGA TCGGTGCTGC 
AACACGTGTG TGGATGAGGA AGACTACGAT CAATTTCGGC TGATGGCGGG AATGCGCAGA 
CAGAGCTGTG ATTCTCTGGC TCTGGCTTTG TGTGACCCGA TGCGCAGACG CAGCTGCGAG 
GAGGACAGTC TCCCAGACAG CGGCATGTCT ACCCTGCGCT CCAATATCGG ATATGATTAT 
GAGCATGAGA GAACATCCAG TCCTGTGGAG GCAGAAGACA GCAACACCAC AGTGTCTGGA 
GACACGTCTG ATACTGAGGT TGTGGAAATG CGAGAAGAGG CTGCGTCAGG GTCGGACAGC 
GAATCCGTTC AGAGCTGGAT GCCATCAAAG CCTGTTCAGA CGGATGACGT TTCTGCTTCA 
GGGAAGAAAT GCCTTTCTGC TATTTTCACA GAGAGAGAGC AGGATGAGCT GGCCGTGTCT 
CAGTCTCCAT CTGAGAAGGC TTACAGGATC GTGCTGGCAG GAGACGCTGC AGTGGGAAAG 
TCCAGCTTTC TTCTCCGTCT CTGTAAGAAT GAGTTCAAAG GCAACACCAG CGCCACACTG 
GGTGTTGATT TCCAGATGAA AACTCTGGTT GTGGATGGCG TTCCAACAGT GCTGCAGTTA 
TGGGACACGG CTGGACAGGA AAGGTTTCGG AGCATTGCTA AGTCATACTT CAGGAGAGCG 
GATGGAGTTC TTCTGCTGTA CGACGTCACC TGTGAGAAGA GCTTTCTGAA TGTGCGAGAA 
TGGGTCGACA TCATCGAGGA TGTTTCTCAG GATGACATTC CCATCATGCT AGTGGGCAAT 
AAGACAGATC TGAGGAAGGA GGCTCTTCAG GACGGAGTCA CCTGCATTCC AACCAGCTAT 
GGAGAGAAAC TGGCCATGAC ATACAGTGCC TTATTCTGTG AGACGAGTGC AAAAGATGGA 
TCCAACATCA TTGAAGCTGT TCTTCATTTA GCCCGAGAGG TGACCAAACA CGCCGACGAT 
TACAAAGAGC CTGTGTCAGT GGCCAAACTG TCTGGAAATC ATACCAAGAA GATGTCCAGT 
CCAAACTGCT GCATGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASEF ( XM_694243.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_699335.4 Danio rerio RAS and EF-hand domain containing (rasef), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14655
Clone ID Related Accession (Same CDS sequence)	XM_694243.8 , XM_017357544.1 , XM_694243.7
Accession Version	XM_694243.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras and EF-hand domain-containing protein
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_694243.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG AGGCGCAGAT GCAGCGACTG CGCGAGCTCT TCCACGCCTG TGATGTGGAT 
CATTCAGGAC GGATCGAGAA ATGGGAGTTT GAGAAAATAT GCTCGGAGCT TCACGTCCAG 
TCCAGCGAGA TCGATTCGCT TTTCTCCAGA CTTGACACCG ACGAAGACGG GACGATAAAC 
CTGGAGGAGT TCATGGAGGG CTTTCAGGAG TCGCACCTGC TGAAGGAGCC GGAGATGAGC 
GGAGAGCCGG CCGGGGAAAC TTCCTCTGCC GCCTGGGAAG ACTTTAAATC GCGCCTTGGC 
GATCAGTTCA AATTCATCCC CAGGATTGAC CAGGTAGCAA CTCTGTACCA GAACATCCGT 
TTGACGGAGC CCAGAATGGT CCCTCAGTAT GAAAGGGTTC TTGTCAGTTT CATCAAGGAG 
ATCCGGATAC TGAACACAGA AATGGAGCAT CTGGCGCTGG CTGTCAAGAG AGCTCAGGAT 
CAGATGTCTA TGCAGCTGAG TGAGATGGAG GAAGAGATGG ATCACCGAAT CCACACCGCA 
GAGAGAAACA CACGTCTGCA GGAGTCTAAA AAAGCTGAGG CGACTCTGAG CAGCATGAAA 
CACCAGTATG ACTCTCAGAT TTGTGAACTC CAGCAGAAGA TTCAGACTCT GCAGATGATG 
GAGAACCAGA ACAGGAGCGT GAGCCTGAAA GAGGAAACAT CGGCACTTAA ACGGCAGAAC 
AACGAGCTCA TGCTGCAAAA CCAGAAGCTG AAGCAGGAGC TGTTGGAGTC CCACACTAAT 
GTTGCGTTCT TGCAAAGCGA GCTGGACTCT CTGAAGAGTG ATCTGACAGA CCAGAGCATC 
AACTGTGAAC GAGACGAGGC TCTTATGAAG TGCTTCTCTG AGGAGAGAGA TAACCTGGAG 
AGACAGATCG AGATGCTTCA GGTAGCAAAC AGAAAGCTGC ACGACAGTAA TGACAGTCTG 
CGTACGGCGC TGGAGAGCAG TCAGAGCAAG AGCAAGACTC AGCGTGTTAG CGGACTCTCA 
CCTGGAGTTT ACGTGAACAC CAGAAAGAAT GTCTGCCACT CTTATTTTGA TCGGTGCTGC 
AACACGTGTG TGGATGAGGA AGACTACGAT CAATTTCGGC TGATGGCGGG AATGCGCAGA 
CAGAGCTGTG ATTCTCTGGC TCTGGCTTTG TGTGACCCGA TGCGCAGACG CAGCTGCGAG 
GAGGACAGTC TCCCAGACAG CGGCATGTCT ACCCTGCGCT CCAATATCGG ATATGATTAT 
GAGCATGAGA GAACATCCAG TCCTGTGGAG GCAGAAGACA GCAACACCAC AGTGTCTGGA 
GACACGTCTG ATACTGAGGT TGTGGAAATG CGAGAAGAGG CTGCGTCAGG GTCGGACAGC 
GAATCCGTTC AGAGCTGGAT GCCATCAAAG CCTGTTCAGA CGGATGACGT TTCTGCTTCA 
GGGAAGAAAT GCCTTTCTGC TATTTTCACA GAGAGAGAGC AGGATGAGCT GGCCGTGTCT 
CAGTCTCCAT CTGAGAAGGC TTACAGGATC GTGCTGGCAG GAGACGCTGC AGTGGGAAAG 
TCCAGCTTTC TTCTCCGTCT CTGTAAGAAT GAGTTCAAAG GCAACACCAG CGCCACACTG 
GGTGTTGATT TCCAGATGAA AACTCTGGTT GTGGATGGCG TTCCAACAGT GCTGCAGTTA 
TGGGACACGG CTGGACAGGA AAGGTTTCGG AGCATTGCTA AGTCATACTT CAGGAGAGCG 
GATGGAGTTC TTCTGCTGTA CGACGTCACC TGTGAGAAGA GCTTTCTGAA TGTGCGAGAA 
TGGGTCGACA TCATCGAGGA TGTTTCTCAG GATGACATTC CCATCATGCT AGTGGGCAAT 
AAGACAGATC TGAGGAAGGA GGCTCTTCAG GACGGAGTCA CCTGCATTCC AACCAGCTAT 
GGAGAGAAAC TGGCCATGAC ATACAGTGCC TTATTCTGTG AGACGAGTGC AAAAGATGGA 
TCCAACATCA TTGAAGCTGT TCTTCATTTA GCCCGAGAGG TGACCAAACA CGCCGACGAT 
TACAAAGAGC CTGTGTCAGT GGCCAAACTG TCTGGAAATC ATACCAAGAA GATGTCCAGT 
CCAAACTGCT GCATGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_699335.4
CDS	81..2201
Translation	MGSEAQMQRLRELFHACDVDHSGRIEKWEFEKICSELHVQSSEIDSLFSRLDTDEDGTINLEEFMEGFQESHLLKEPEMSGEPAGETSSAAWEDFKSRLGDQFKFIPRIDQVATLYQNIRLTEPRMVPQYERVLVSFIKEIRILNTEMEHLALAVKRAQDQMSMQLSEMEEEMDHRIHTAERNTRLQESKKAEATLSSMKHQYDSQICELQQKIQTLQMMENQNRSVSLKEETSALKRQNNELMLQNQKLKQELLESHTNVAFLQSELDSLKSDLTDQSINCERDEALMKCFSEERDNLERQIEMLQVANRKLHDSNDSLRTALESSQSKSKTQRVSGLSPGVYVNTRKNVCHSYFDRCCNTCVDEEDYDQFRLMAGMRRQSCDSLALALCDPMRRRSCEEDSLPDSGMSTLRSNIGYDYEHERTSSPVEAEDSNTTVSGDTSDTEVVEMREEAASGSDSESVQSWMPSKPVQTDDVSASGKKCLSAIFTEREQDELAVSQSPSEKAYRIVLAGDAAVGKSSFLLRLCKNEFKGNTSATLGVDFQMKTLVVDGVPTVLQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWVDIIEDVSQDDIPIMLVGNKTDLRKEALQDGVTCIPTSYGEKLAMTYSALFCETSAKDGSNIIEAVLHLAREVTKHADDYKEPVSVAKLSGNHTKKMSSPNCCMG

Target ORF information:

RefSeq Version	XM_694243.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RAS and EF-hand domain containing (rasef), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_694243.7

ORF Insert Sequence:

ATGGGCTCTG AGGCGCAGAT GCAGCGACTG CGCGAGCTCT TCCACGCCTG TGATGTGGAT 
CATTCAGGAC GGATCGAGAA ATGGGAGTTT GAGAAAATAT GCTCGGAGCT TCACGTCCAG 
TCCAGCGAGA TCGATTCGCT TTTCTCCAGA CTTGACACCG ACGAAGACGG GACGATAAAC 
CTGGAGGAGT TCATGGAGGG CTTTCAGGAG TCGCACCTGC TGAAGGAGCC GGAGATGAGC 
GGAGAGCCGG CCGGGGAAAC TTCCTCTGCC GCCTGGGAAG ACTTTAAATC GCGCCTTGGC 
GATCAGTTCA AATTCATCCC CAGGATTGAC CAGGTAGCAA CTCTGTACCA GAACATCCGT 
TTGACGGAGC CCAGAATGGT CCCTCAGTAT GAAAGGGTTC TTGTCAGTTT CATCAAGGAG 
ATCCGGATAC TGAACACAGA AATGGAGCAT CTGGCGCTGG CTGTCAAGAG AGCTCAGGAT 
CAGATGTCTA TGCAGCTGAG TGAGATGGAG GAAGAGATGG ATCACCGAAT CCACACCGCA 
GAGAGAAACA CACGTCTGCA GGAGTCTAAA AAAGCTGAGG CGACTCTGAG CAGCATGAAA 
CACCAGTATG ACTCTCAGAT TTGTGAACTC CAGCAGAAGA TTCAGACTCT GCAGATGATG 
GAGAACCAGA ACAGGAGCGT GAGCCTGAAA GAGGAAACAT CGGCACTTAA ACGGCAGAAC 
AACGAGCTCA TGCTGCAAAA CCAGAAGCTG AAGCAGGAGC TGTTGGAGTC CCACACTAAT 
GTTGCGTTCT TGCAAAGCGA GCTGGACTCT CTGAAGAGTG ATCTGACAGA CCAGAGCATC 
AACTGTGAAC GAGACGAGGC TCTTATGAAG TGCTTCTCTG AGGAGAGAGA TAACCTGGAG 
AGACAGATCG AGATGCTTCA GGTAGCAAAC AGAAAGCTGC ACGACAGTAA TGACAGTCTG 
CGTACGGCGC TGGAGAGCAG TCAGAGCAAG AGCAAGACTC AGCGTGTTAG CGGACTCTCA 
CCTGGAGTTT ACGTGAACAC CAGAAAGAAT GTCTGCCACT CTTATTTTGA TCGGTGCTGC 
AACACGTGTG TGGATGAGGA AGACTACGAT CAATTTCGGC TGATGGCGGG AATGCGCAGA 
CAGAGCTGTG ATTCTCTGGC TCTGGCTTTG TGTGACCCGA TGCGCAGACG CAGCTGCGAG 
GAGGACAGTC TCCCAGACAG CGGCATGTCT ACCCTGCGCT CCAATATCGG ATATGATTAT 
GAGCATGAGA GAACATCCAG TCCTGTGGAG GCAGAAGACA GCAACACCAC AGTGTCTGGA 
GACACGTCTG ATACTGAGGT TGTGGAAATG CGAGAAGAGG CTGCGTCAGG GTCGGACAGC 
GAATCCGTTC AGAGCTGGAT GCCATCAAAG CCTGTTCAGA CGGATGACGT TTCTGCTTCA 
GGGAAGAAAT GCCTTTCTGC TATTTTCACA GAGAGAGAGC AGGATGAGCT GGCCGTGTCT 
CAGTCTCCAT CTGAGAAGGC TTACAGGATC GTGCTGGCAG GAGACGCTGC AGTGGGAAAG 
TCCAGCTTTC TTCTCCGTCT CTGTAAGAAT GAGTTCAAAG GCAACACCAG CGCCACACTG 
GGTGTTGATT TCCAGATGAA AACTCTGGTT GTGGATGGCG TTCCAACAGT GCTGCAGTTA 
TGGGACACGG CTGGACAGGA AAGGTTTCGG AGCATTGCTA AGTCATACTT CAGGAGAGCG 
GATGGAGTTC TTCTGCTGTA CGACGTCACC TGTGAGAAGA GCTTTCTGAA TGTGCGAGAA 
TGGGTCGACA TCATCGAGGA TGTTTCTCAG GATGACATTC CCATCATGCT AGTGGGCAAT 
AAGACAGATC TGAGGAAGGA GGCTCTTCAG GACGGAGTCA CCTGCATTCC AACCAGCTAT 
GGAGAGAAAC TGGCCATGAC ATACAGTGCC TTATTCTGTG AGACGAGTGC AAAAGATGGA 
TCCAACATCA TTGAAGCTGT TCTTCATTTA GCCCGAGAGG TGACCAAACA CGCCGACGAT 
TACAAAGAGC CTGTGTCAGT GGCCAAACTG TCTGGAAATC ATACCAAGAA GATGTCCAGT 
CCAAACTGCT GCATGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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RASEF cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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