phldb1b cDNA ORF clone, Danio rerio(zebrafish)

The following phldb1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phldb1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa45943 XM_021466370.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa45944 XM_021466371.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa45940 XM_021466368.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa45941 XM_021466369.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa55244 XM_017351158.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa149045 XM_021466360.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa149046 XM_021466363.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa149047 XM_021466365.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa149048 XM_021466366.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa149049 XM_021466367.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa149050 XM_021466372.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa149051 XM_021466373.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa45936 XM_021466359.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa45937 XM_021466361.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa45938 XM_021466362.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa45939 XM_021466364.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55240 XM_017351154.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55240 XM_017351153.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55241 XM_017351155.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55242 XM_017351156.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa55243 XM_017351157.1
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa45942 XM_005157452.4
Latest version!
Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa45942 XM_005157452.3 Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa45943
    Clone ID Related Accession (Same CDS sequence) XM_021466370.1
    Accession Version XM_021466370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2784bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGAAC GGCAGCGGCT GGAGACAATC TTGAACCTCT GCGCTGAGTA TAACAAGGGC 
    GACGGGCCGC CGGGTGATTT GGGCAGGATG GGCGTCTCTG GGCTTGGCTC GAGGGACGGG
    CTTAACGTCA GGCGTCCTTC AATGGACAGC GTGAACAGCG CTTCACTAAG AGCGACTCAA
    AGACAACGGG AAAGCCAAGA GGACAACCTG AAGGAGGATA GCAGTAGCAC TGAGAGTGCG
    CAGCTGGACG GACGGACACC TGCTCTGCTA AATGGACAGA ATGGACAGGC TGAGGAGTCT
    CTGGGTTCGG GCAGCATTGG CCGTCTGGAG CTGGGCTACC TGGAGGAGGA GCGGGTTCGA
    GTGCTGGCAA AGGTAGATGA GCTCAAAGCT CGAATCACAG AACTGGAGCA GCAACTTCAG
    GAGTCTAAAC AAGAGGCGGA GATGGAGAGA GCTCTGCTGC AGGGAGAAAG ACAGGCTGAG
    ATGGAGCAAA TCGAGACTGA GACCGAGATT ATCAACCAGC TGCAGCGCAA ACTCACTGAA
    CTGGAGAGCA GCATCCAGAG AGAGAAGGAC AAGGCATGCC ATTCTTCCAG ATGGCCACTC
    AAGGCCCATT CAGGTCTCGA TTGCCATCCT CAGGAGAGGG CTAATGTTGA CGCGGAGCGG
    GAAGCCCTGG AGAGGCTTCA GGATGGGTAC AATGAGTTGA CGAGCCAGCT ACATAACTGC
    CCTGAGTCTC TGAGGGAACA GTTACAGGAA CAGCTCAAGA GGGAAGCTGA GACTCTTGAA
    GCAGCGACAA AGCAGTTTGA AGACCTGGAG TTTCAGCAGT TGGAGAGGGA GAGCAGTCTG
    GAGGAGGAGC GCGAGACCAT CAGCCAGCAG CTCATGCAGG ACAGAGCTCA ATACCACAGC
    AGTGTCGCCA AGAGAAAGGA AAAGGTGGTG GCATTGGAGA GCCAGGCGAG CCAGCTGGGA
    ATACAGGCCG CTCAGGAGTG TGAGAGACTG GCCAAGGACA GAACCATCAC CTTGCAGATG
    CTACACAAGG AAAAGGAAAG GCTGGCCAGT CTGGAGAGAA GATACCAGAG TTTAACTGGT
    GGAAAGACGT TCCCCAAAAG CTCCAATACT ATAAAGGAGG AGTACTTGAA GCTGTCGGAT
    GTGTATAAGA TGTATGGGAG TGAATGCCAT GACATCCAGC TCGCCAGCGC TTCCTCACAC
    GGCCTCTCGC TTGCCCTTGA CCCAGCTGAA ACTGCCCCGC GTGAGGAGTA TGTGACGGTC
    AGCCAATTAA ACCAGATGTA CGGGATGCCA AAGGTGGAGT CTTCCCCTAC CTCACCCGTC
    CAATCAGTGC AGCCCTCTCT CGCAGACTCC ACCTTCCCCC GCCTCCCTTC CCCTCATGGC
    TCCTCCCTCT CTCTCTCCTT CGAGCGTCAG TCAGACTGGG GCAGGCCTCA GATGCCCGTG
    CTAGATTTAG AGCACTGGTA CCAGGAGGTA ATGGCAGCTG GAGATGTAAA CCAGCTCTGC
    CCTCCTCCAT TGCCGGCTAA AGCTTTCTCA TCATCATCAT CATCACCACG CAGGCCTGTG
    CAGGTGTACA GATCTCGACT GGACAGTGAT TGCAGCCAAT CCTCACTTAG ACCGAAAGTC
    AGCGCCGGTT TGTCTCCTAC ATACACGACG GCCACGCTGG GACGAAACAG TCCTGCCAGG
    AGTCCTCTGA TGGTGGCCAA CAGCACCGGA AGTCTCCCAC GCAACCTGGC AGCCACCCTG
    CAGGACATCG AGACCAAGAG ACAGCTGGCT CTTCAGCAGA AAGGTCAGCA GGTTATCGAG
    GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG
    TGGGAGGCGC TGCACGGCTC TCAAACGCAG CTCCACCAGC CCTTCTCATC AAGCCCAGCA
    CCGGCGCTTC ACCACTCCAT CCTGCACCAC ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC
    GACACGCTAA GCCTGGAGAG CTCGGACAGC ATGGACACCA GCATCTCTAC TGGAAACAAC
    TCGGCCTGCT CGCCGGACAA CATGTCCAGT GCCAGCGGTA TGGATGCGTT GAAGATTGAA
    GAGATGGAGA AGATGTTGAA GGAGGCGCAG CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA
    GAGCGAGAGA CTCAGAGCCG TAAACTGATG CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG
    GCCGAGAAGA GGCTGCAGGA GGAGACGCTG CACAGACAGC AGCTCATCGA GAAGGAGGTC
    AAGATGAGGG CCAGAAACTT CTCTCAGGCC CGTCCGATGA CCCGCTACCT GCCCATCCGC
    AAAGAGGAGT TTGACCTGCG TTCGCACATC GAGTCTTCAG GCCACAGCGT AGACACCTGC
    TACCACGTCA TCCTCACCGA GAAGATGTGC AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG
    ATCAAATCCT GGAAGAAGCG CTGGTTCGTC TTTGATCGCC TCAAAAGGAC GTTCTCTTAC
    TACGCCGACA AACACGAGAG CAAGCTGAAG GGTGTGATTT ACTTCCAGGC CATCGAGGAG
    GTTTACTACG ATCACCTGCG CAGCGCCACA AAGAAAGGAT TTTTCAATCT GAATCTGACA
    AACAGCCCGA CCCCGACGCT GACCTTCTGT GTTAAAACAC ACGACAGACT GTATTACATG
    GTGGCTCCGT CCGCAGAGGC CATGAGGATA TGGATGGATG TGATTGTGAC AGGAGCCGAG
    GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322045.1
    CDS124..2907
    Translation

    Target ORF information:

    RefSeq Version XM_021466370.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGAAC GGCAGCGGCT GGAGACAATC TTGAACCTCT GCGCTGAGTA TAACAAGGGC 
    GACGGGCCGC CGGGTGATTT GGGCAGGATG GGCGTCTCTG GGCTTGGCTC GAGGGACGGG
    CTTAACGTCA GGCGTCCTTC AATGGACAGC GTGAACAGCG CTTCACTAAG AGCGACTCAA
    AGACAACGGG AAAGCCAAGA GGACAACCTG AAGGAGGATA GCAGTAGCAC TGAGAGTGCG
    CAGCTGGACG GACGGACACC TGCTCTGCTA AATGGACAGA ATGGACAGGC TGAGGAGTCT
    CTGGGTTCGG GCAGCATTGG CCGTCTGGAG CTGGGCTACC TGGAGGAGGA GCGGGTTCGA
    GTGCTGGCAA AGGTAGATGA GCTCAAAGCT CGAATCACAG AACTGGAGCA GCAACTTCAG
    GAGTCTAAAC AAGAGGCGGA GATGGAGAGA GCTCTGCTGC AGGGAGAAAG ACAGGCTGAG
    ATGGAGCAAA TCGAGACTGA GACCGAGATT ATCAACCAGC TGCAGCGCAA ACTCACTGAA
    CTGGAGAGCA GCATCCAGAG AGAGAAGGAC AAGGCATGCC ATTCTTCCAG ATGGCCACTC
    AAGGCCCATT CAGGTCTCGA TTGCCATCCT CAGGAGAGGG CTAATGTTGA CGCGGAGCGG
    GAAGCCCTGG AGAGGCTTCA GGATGGGTAC AATGAGTTGA CGAGCCAGCT ACATAACTGC
    CCTGAGTCTC TGAGGGAACA GTTACAGGAA CAGCTCAAGA GGGAAGCTGA GACTCTTGAA
    GCAGCGACAA AGCAGTTTGA AGACCTGGAG TTTCAGCAGT TGGAGAGGGA GAGCAGTCTG
    GAGGAGGAGC GCGAGACCAT CAGCCAGCAG CTCATGCAGG ACAGAGCTCA ATACCACAGC
    AGTGTCGCCA AGAGAAAGGA AAAGGTGGTG GCATTGGAGA GCCAGGCGAG CCAGCTGGGA
    ATACAGGCCG CTCAGGAGTG TGAGAGACTG GCCAAGGACA GAACCATCAC CTTGCAGATG
    CTACACAAGG AAAAGGAAAG GCTGGCCAGT CTGGAGAGAA GATACCAGAG TTTAACTGGT
    GGAAAGACGT TCCCCAAAAG CTCCAATACT ATAAAGGAGG AGTACTTGAA GCTGTCGGAT
    GTGTATAAGA TGTATGGGAG TGAATGCCAT GACATCCAGC TCGCCAGCGC TTCCTCACAC
    GGCCTCTCGC TTGCCCTTGA CCCAGCTGAA ACTGCCCCGC GTGAGGAGTA TGTGACGGTC
    AGCCAATTAA ACCAGATGTA CGGGATGCCA AAGGTGGAGT CTTCCCCTAC CTCACCCGTC
    CAATCAGTGC AGCCCTCTCT CGCAGACTCC ACCTTCCCCC GCCTCCCTTC CCCTCATGGC
    TCCTCCCTCT CTCTCTCCTT CGAGCGTCAG TCAGACTGGG GCAGGCCTCA GATGCCCGTG
    CTAGATTTAG AGCACTGGTA CCAGGAGGTA ATGGCAGCTG GAGATGTAAA CCAGCTCTGC
    CCTCCTCCAT TGCCGGCTAA AGCTTTCTCA TCATCATCAT CATCACCACG CAGGCCTGTG
    CAGGTGTACA GATCTCGACT GGACAGTGAT TGCAGCCAAT CCTCACTTAG ACCGAAAGTC
    AGCGCCGGTT TGTCTCCTAC ATACACGACG GCCACGCTGG GACGAAACAG TCCTGCCAGG
    AGTCCTCTGA TGGTGGCCAA CAGCACCGGA AGTCTCCCAC GCAACCTGGC AGCCACCCTG
    CAGGACATCG AGACCAAGAG ACAGCTGGCT CTTCAGCAGA AAGGTCAGCA GGTTATCGAG
    GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG
    TGGGAGGCGC TGCACGGCTC TCAAACGCAG CTCCACCAGC CCTTCTCATC AAGCCCAGCA
    CCGGCGCTTC ACCACTCCAT CCTGCACCAC ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC
    GACACGCTAA GCCTGGAGAG CTCGGACAGC ATGGACACCA GCATCTCTAC TGGAAACAAC
    TCGGCCTGCT CGCCGGACAA CATGTCCAGT GCCAGCGGTA TGGATGCGTT GAAGATTGAA
    GAGATGGAGA AGATGTTGAA GGAGGCGCAG CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA
    GAGCGAGAGA CTCAGAGCCG TAAACTGATG CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG
    GCCGAGAAGA GGCTGCAGGA GGAGACGCTG CACAGACAGC AGCTCATCGA GAAGGAGGTC
    AAGATGAGGG CCAGAAACTT CTCTCAGGCC CGTCCGATGA CCCGCTACCT GCCCATCCGC
    AAAGAGGAGT TTGACCTGCG TTCGCACATC GAGTCTTCAG GCCACAGCGT AGACACCTGC
    TACCACGTCA TCCTCACCGA GAAGATGTGC AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG
    ATCAAATCCT GGAAGAAGCG CTGGTTCGTC TTTGATCGCC TCAAAAGGAC GTTCTCTTAC
    TACGCCGACA AACACGAGAG CAAGCTGAAG GGTGTGATTT ACTTCCAGGC CATCGAGGAG
    GTTTACTACG ATCACCTGCG CAGCGCCACA AAGAAAGGAT TTTTCAATCT GAATCTGACA
    AACAGCCCGA CCCCGACGCT GACCTTCTGT GTTAAAACAC ACGACAGACT GTATTACATG
    GTGGCTCCGT CCGCAGAGGC CATGAGGATA TGGATGGATG TGATTGTGAC AGGAGCCGAG
    GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45944
    Clone ID Related Accession (Same CDS sequence) XM_021466371.1
    Accession Version XM_021466371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2595bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGGAAC GGCAGCGGCT GGAGACAATC TTGAACCTCT GCGCTGAGTA TAACAAGGGC 
    GACGGGCCGC CGGGTGATTT GGGCAGGATG GGCGTCTCTG GGCTTGGCTC GAGGGACGGG
    CTTAACGTCA GGCGTCCTTC AATGGACAGC GTGAACAGCG CTTCACTAAG AGCGACTCAA
    AGACAACGGG AAAGCCAAGA GGACAACCTG AAGGAGGATA GCAGTAGCAC TGAGAGTGCG
    CAGCTGGACG GACGGACACC TGCTCTGCTA AATGGACAGA ATGGACAGGC TGAGGAGTCT
    CTGGGTTCGG GCAGCATTGG CCGTCTGGAG CTGGGCTACC TGGAGGAGGA GCGGGTTCGA
    GTGCTGGCAA AGGTAGATGA GCTCAAAGCT CGAATCACAG AACTGGAGCA GCAACTTCAG
    GAGTCTAAAC AAGAGGCGGA GATGGAGAGA GCTCTGCTGC AGGGAGAAAG ACAGGCTGAG
    ATGGAGCAAA TCGAGACTGA GACCGAGATT ATCAACCAGC TGCAGCGCAA ACTCACTGAA
    CTGGAGAGCA GCATCCAGAG AGAGAAGGAC AAGGAAGCTG AGACTCTTGA AGCAGCGACA
    AAGCAGTTTG AAGACCTGGA GTTTCAGCAG TTGGAGAGGG AGAGCAGTCT GGAGGAGGAG
    CGCGAGACCA TCAGCCAGCA GCTCATGCAG GACAGAGCTC AATACCACAG CAGTGTCGCC
    AAGAGAAAGG AAAAGGTGGT GGCATTGGAG AGCCAGGCGA GCCAGCTGGG AATACAGGCC
    GCTCAGGAGT GTGAGAGACT GGCCAAGGAC AGAACCATCA CCTTGCAGAT GCTACACAAG
    GAAAAGGAAA GGCTGGCCAG TCTGGAGAGA AGATACCAGA GTTTAACTGG TGGAAAGACG
    TTCCCCAAAA GCTCCAATAC TATAAAGGAG GAGTACTTGA AGCTGTCGGA TGTGTATAAG
    ATGTATGGGA GTGAATGCCA TGACATCCAG CTCGCCAGCG CTTCCTCACA CGGCCTCTCG
    CTTGCCCTTG ACCCAGCTGA AACTGCCCCG CGTGAGGAGT ATGTGACGGT CAGCCAATTA
    AACCAGATGT ACGGGATGCC AAAGGTGGAG TCTTCCCCTA CCTCACCCGT CCAATCAGTG
    CAGCCCTCTC TCGCAGACTC CACCTTCCCC CGCCTCCCTT CCCCTCATGG CTCCTCCCTC
    TCTCTCTCCT TCGAGCGTCA GTCAGACTGG GGCAGGCCTC AGATGCCCGT GCTAGATTTA
    GAGCACTGGT ACCAGGAGGT AATGGCAGCT GGAGATGTAA ACCAGCTCTG CCCTCCTCCA
    TTGCCGGCTA AAGCTTTCTC ATCATCATCA TCATCACCAC GCAGGCCTGT GCAGGTGTAC
    AGATCTCGAC TGGACAGTGA TTGCAGCCAA TCCTCACTTA GACCGAAAGT CAGCGCCGGT
    TTGTCTCCTA CATACACGAC GGCCACGCTG GGACGAAACA GTCCTGCCAG GAGTCCTCTG
    ATGGTGGCCA ACAGCACCGG AAGTCTCCCA CGCAACCTGG CAGCCACCCT GCAGGACATC
    GAGACCAAGA GACAGCTGGC TCTTCAGCAG AAAGGTCAGC AGGTTATCGA GGAGCAGCGC
    AGGCGTCTGG CGGAGCTGAA GCAGAAAGCT GCAGTGGAGG CTCAGTGCCA GTGGGAGGCG
    CTGCACGGCT CTCAAACGCA GCTCCACCAG CCCTTCTCAT CAAGCCCAGC ACCGGCGCTT
    CACCACTCCA TCCTGCACCA CACAGCGCCC CCTTGCGGAG AGCAGCCGTA CGACACGCTA
    AGCCTGGAGA GCTCGGACAG CATGGACACC AGCATCTCTA CTGGAAACAA CTCGGCCTGC
    TCGCCGGACA ACATGTCCAG TGCCAGCGGT ATGGATGCGT TGAAGATTGA AGAGATGGAG
    AAGATGTTGA AGGAGGCGCA GCTGGAAAAA GCTCGGCTCA TAGAGTCTAG AGAGCGAGAG
    ACTCAGAGCC GTAAACTGAT GCTGGAGGAG GAGCGGAGGA GGAGAGAGGA GGCCGAGAAG
    AGGCTGCAGG AGGAGACGCT GCACAGACAG CAGCTCATCG AGAAGGAGGT CAAGATGAGG
    GCCAGAAACT TCTCTCAGGC CCGTCCGATG ACCCGCTACC TGCCCATCCG CAAAGAGGAG
    TTTGACCTGC GTTCGCACAT CGAGTCTTCA GGCCACAGCG TAGACACCTG CTACCACGTC
    ATCCTCACCG AGAAGATGTG CAAGGGCTTT CTGGTCAAGA TGGGAGGCAA GATCAAATCC
    TGGAAGAAGC GCTGGTTCGT CTTTGATCGC CTCAAAAGGA CGTTCTCTTA CTACGCCGAC
    AAACACGAGA GCAAGCTGAA GGGTGTGATT TACTTCCAGG CCATCGAGGA GGTTTACTAC
    GATCACCTGC GCAGCGCCAC AAAGAAAGGA TTTTTCAATC TGAATCTGAC AAACAGCCCG
    ACCCCGACGC TGACCTTCTG TGTTAAAACA CACGACAGAC TGTATTACAT GGTGGCTCCG
    TCCGCAGAGG CCATGAGGAT ATGGATGGAT GTGATTGTGA CAGGAGCCGA GGGCTACACA
    CAGTTCATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322046.1
    CDS124..2718
    Translation

    Target ORF information:

    RefSeq Version XM_021466371.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAGGGAAC GGCAGCGGCT GGAGACAATC TTGAACCTCT GCGCTGAGTA TAACAAGGGC 
    GACGGGCCGC CGGGTGATTT GGGCAGGATG GGCGTCTCTG GGCTTGGCTC GAGGGACGGG
    CTTAACGTCA GGCGTCCTTC AATGGACAGC GTGAACAGCG CTTCACTAAG AGCGACTCAA
    AGACAACGGG AAAGCCAAGA GGACAACCTG AAGGAGGATA GCAGTAGCAC TGAGAGTGCG
    CAGCTGGACG GACGGACACC TGCTCTGCTA AATGGACAGA ATGGACAGGC TGAGGAGTCT
    CTGGGTTCGG GCAGCATTGG CCGTCTGGAG CTGGGCTACC TGGAGGAGGA GCGGGTTCGA
    GTGCTGGCAA AGGTAGATGA GCTCAAAGCT CGAATCACAG AACTGGAGCA GCAACTTCAG
    GAGTCTAAAC AAGAGGCGGA GATGGAGAGA GCTCTGCTGC AGGGAGAAAG ACAGGCTGAG
    ATGGAGCAAA TCGAGACTGA GACCGAGATT ATCAACCAGC TGCAGCGCAA ACTCACTGAA
    CTGGAGAGCA GCATCCAGAG AGAGAAGGAC AAGGAAGCTG AGACTCTTGA AGCAGCGACA
    AAGCAGTTTG AAGACCTGGA GTTTCAGCAG TTGGAGAGGG AGAGCAGTCT GGAGGAGGAG
    CGCGAGACCA TCAGCCAGCA GCTCATGCAG GACAGAGCTC AATACCACAG CAGTGTCGCC
    AAGAGAAAGG AAAAGGTGGT GGCATTGGAG AGCCAGGCGA GCCAGCTGGG AATACAGGCC
    GCTCAGGAGT GTGAGAGACT GGCCAAGGAC AGAACCATCA CCTTGCAGAT GCTACACAAG
    GAAAAGGAAA GGCTGGCCAG TCTGGAGAGA AGATACCAGA GTTTAACTGG TGGAAAGACG
    TTCCCCAAAA GCTCCAATAC TATAAAGGAG GAGTACTTGA AGCTGTCGGA TGTGTATAAG
    ATGTATGGGA GTGAATGCCA TGACATCCAG CTCGCCAGCG CTTCCTCACA CGGCCTCTCG
    CTTGCCCTTG ACCCAGCTGA AACTGCCCCG CGTGAGGAGT ATGTGACGGT CAGCCAATTA
    AACCAGATGT ACGGGATGCC AAAGGTGGAG TCTTCCCCTA CCTCACCCGT CCAATCAGTG
    CAGCCCTCTC TCGCAGACTC CACCTTCCCC CGCCTCCCTT CCCCTCATGG CTCCTCCCTC
    TCTCTCTCCT TCGAGCGTCA GTCAGACTGG GGCAGGCCTC AGATGCCCGT GCTAGATTTA
    GAGCACTGGT ACCAGGAGGT AATGGCAGCT GGAGATGTAA ACCAGCTCTG CCCTCCTCCA
    TTGCCGGCTA AAGCTTTCTC ATCATCATCA TCATCACCAC GCAGGCCTGT GCAGGTGTAC
    AGATCTCGAC TGGACAGTGA TTGCAGCCAA TCCTCACTTA GACCGAAAGT CAGCGCCGGT
    TTGTCTCCTA CATACACGAC GGCCACGCTG GGACGAAACA GTCCTGCCAG GAGTCCTCTG
    ATGGTGGCCA ACAGCACCGG AAGTCTCCCA CGCAACCTGG CAGCCACCCT GCAGGACATC
    GAGACCAAGA GACAGCTGGC TCTTCAGCAG AAAGGTCAGC AGGTTATCGA GGAGCAGCGC
    AGGCGTCTGG CGGAGCTGAA GCAGAAAGCT GCAGTGGAGG CTCAGTGCCA GTGGGAGGCG
    CTGCACGGCT CTCAAACGCA GCTCCACCAG CCCTTCTCAT CAAGCCCAGC ACCGGCGCTT
    CACCACTCCA TCCTGCACCA CACAGCGCCC CCTTGCGGAG AGCAGCCGTA CGACACGCTA
    AGCCTGGAGA GCTCGGACAG CATGGACACC AGCATCTCTA CTGGAAACAA CTCGGCCTGC
    TCGCCGGACA ACATGTCCAG TGCCAGCGGT ATGGATGCGT TGAAGATTGA AGAGATGGAG
    AAGATGTTGA AGGAGGCGCA GCTGGAAAAA GCTCGGCTCA TAGAGTCTAG AGAGCGAGAG
    ACTCAGAGCC GTAAACTGAT GCTGGAGGAG GAGCGGAGGA GGAGAGAGGA GGCCGAGAAG
    AGGCTGCAGG AGGAGACGCT GCACAGACAG CAGCTCATCG AGAAGGAGGT CAAGATGAGG
    GCCAGAAACT TCTCTCAGGC CCGTCCGATG ACCCGCTACC TGCCCATCCG CAAAGAGGAG
    TTTGACCTGC GTTCGCACAT CGAGTCTTCA GGCCACAGCG TAGACACCTG CTACCACGTC
    ATCCTCACCG AGAAGATGTG CAAGGGCTTT CTGGTCAAGA TGGGAGGCAA GATCAAATCC
    TGGAAGAAGC GCTGGTTCGT CTTTGATCGC CTCAAAAGGA CGTTCTCTTA CTACGCCGAC
    AAACACGAGA GCAAGCTGAA GGGTGTGATT TACTTCCAGG CCATCGAGGA GGTTTACTAC
    GATCACCTGC GCAGCGCCAC AAAGAAAGGA TTTTTCAATC TGAATCTGAC AAACAGCCCG
    ACCCCGACGC TGACCTTCTG TGTTAAAACA CACGACAGAC TGTATTACAT GGTGGCTCCG
    TCCGCAGAGG CCATGAGGAT ATGGATGGAT GTGATTGTGA CAGGAGCCGA GGGCTACACA
    CAGTTCATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45940
    Clone ID Related Accession (Same CDS sequence) XM_021466368.1
    Accession Version XM_021466368.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4419bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGCATGCCA TTCTTCCAGA TGGCCACTCA AGGCCCATTC AGGTCTCGAT
    TGCCATCCTC AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGT GTACAGATCT CGACTGGACA GTGATTGCAG CCAATCCTCA
    CTTAGACCGA AAGTCAGCGC CGGTTTGTCT CCTACATACA CGACGGCCAC GCTGGGACGA
    AACAGTCCTG CCAGGAGTCC TCTGATGGTG GCCAACAGCA CCGGAAGTCT CCCACGCAAC
    CTGGCAGCCA CCCTGCAGGA CATCGAGACC AAGAGACAGC TGGCTCTTCA GCAGAAAGGT
    CAGCAGGTTA TCGAGGAGCA GCGCAGGCGT CTGGCGGAGC TGAAGCAGAA AGCTGCAGTG
    GAGGCTCAGT GCCAGTGGGA GGCGCTGCAC GGCTCTCAAA CGCAGCTCCA CCAGCCCTTC
    TCATCAAGCC CAGCACCGGC GCTTCACCAC TCCATCCTGC ACCACACAGC GCCCCCTTGC
    GGAGAGCAGC CGTACGACAC GCTAAGCCTG GAGAGCTCGG ACAGCATGGA CACCAGCATC
    TCTACTGGAA ACAACTCGGC CTGCTCGCCG GACAACATGT CCAGTGCCAG CGGTATGGAT
    GCGTTGAAGA TTGAAGAGAT GGAGAAGATG TTGAAGGAGG CGCAGCTGGA AAAAGCTCGG
    CTCATAGAGT CTAGAGAGCG AGAGACTCAG AGCCGTAAAC TGATGCTGGA GGAGGAGCGG
    AGGAGGAGAG AGGAGGCCGA GAAGAGGCTG CAGGAGGAGA CGCTGCACAG ACAGCAGCTC
    ATCGAGAAGG AGGTCAAGAT GAGGGCCAGA AACTTCTCTC AGGCCCGTCC GATGACCCGC
    TACCTGCCCA TCCGCAAAGA GGAGTTTGAC CTGCGTTCGC ACATCGAGTC TTCAGGCCAC
    AGCGTAGACA CCTGCTACCA CGTCATCCTC ACCGAGAAGA TGTGCAAGGG CTTTCTGGTC
    AAGATGGGAG GCAAGATCAA ATCCTGGAAG AAGCGCTGGT TCGTCTTTGA TCGCCTCAAA
    AGGACGTTCT CTTACTACGC CGACAAACAC GAGAGCAAGC TGAAGGGTGT GATTTACTTC
    CAGGCCATCG AGGAGGTTTA CTACGATCAC CTGCGCAGCG CCACAAAGAA AGGATTTTTC
    AATCTGAATC TGACAAACAG CCCGACCCCG ACGCTGACCT TCTGTGTTAA AACACACGAC
    AGACTGTATT ACATGGTGGC TCCGTCCGCA GAGGCCATGA GGATATGGAT GGATGTGATT
    GTGACAGGAG CCGAGGGCTA CACACAGTTC ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322043.1
    CDS472..4890
    Translation

    Target ORF information:

    RefSeq Version XM_021466368.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGCATGCCA TTCTTCCAGA TGGCCACTCA AGGCCCATTC AGGTCTCGAT
    TGCCATCCTC AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGT GTACAGATCT CGACTGGACA GTGATTGCAG CCAATCCTCA
    CTTAGACCGA AAGTCAGCGC CGGTTTGTCT CCTACATACA CGACGGCCAC GCTGGGACGA
    AACAGTCCTG CCAGGAGTCC TCTGATGGTG GCCAACAGCA CCGGAAGTCT CCCACGCAAC
    CTGGCAGCCA CCCTGCAGGA CATCGAGACC AAGAGACAGC TGGCTCTTCA GCAGAAAGGT
    CAGCAGGTTA TCGAGGAGCA GCGCAGGCGT CTGGCGGAGC TGAAGCAGAA AGCTGCAGTG
    GAGGCTCAGT GCCAGTGGGA GGCGCTGCAC GGCTCTCAAA CGCAGCTCCA CCAGCCCTTC
    TCATCAAGCC CAGCACCGGC GCTTCACCAC TCCATCCTGC ACCACACAGC GCCCCCTTGC
    GGAGAGCAGC CGTACGACAC GCTAAGCCTG GAGAGCTCGG ACAGCATGGA CACCAGCATC
    TCTACTGGAA ACAACTCGGC CTGCTCGCCG GACAACATGT CCAGTGCCAG CGGTATGGAT
    GCGTTGAAGA TTGAAGAGAT GGAGAAGATG TTGAAGGAGG CGCAGCTGGA AAAAGCTCGG
    CTCATAGAGT CTAGAGAGCG AGAGACTCAG AGCCGTAAAC TGATGCTGGA GGAGGAGCGG
    AGGAGGAGAG AGGAGGCCGA GAAGAGGCTG CAGGAGGAGA CGCTGCACAG ACAGCAGCTC
    ATCGAGAAGG AGGTCAAGAT GAGGGCCAGA AACTTCTCTC AGGCCCGTCC GATGACCCGC
    TACCTGCCCA TCCGCAAAGA GGAGTTTGAC CTGCGTTCGC ACATCGAGTC TTCAGGCCAC
    AGCGTAGACA CCTGCTACCA CGTCATCCTC ACCGAGAAGA TGTGCAAGGG CTTTCTGGTC
    AAGATGGGAG GCAAGATCAA ATCCTGGAAG AAGCGCTGGT TCGTCTTTGA TCGCCTCAAA
    AGGACGTTCT CTTACTACGC CGACAAACAC GAGAGCAAGC TGAAGGGTGT GATTTACTTC
    CAGGCCATCG AGGAGGTTTA CTACGATCAC CTGCGCAGCG CCACAAAGAA AGGATTTTTC
    AATCTGAATC TGACAAACAG CCCGACCCCG ACGCTGACCT TCTGTGTTAA AACACACGAC
    AGACTGTATT ACATGGTGGC TCCGTCCGCA GAGGCCATGA GGATATGGAT GGATGTGATT
    GTGACAGGAG CCGAGGGCTA CACACAGTTC ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45941
    Clone ID Related Accession (Same CDS sequence) XM_021466369.1
    Accession Version XM_021466369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4401bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGTGTT TCGGCCAATC CGCCTTCTTC CGCTTCAACC ACCCCGAGGA GGCTTTCCGA 
    ATGAAAAGCA TGATGCCCGA GGGAGGACAG ACAGCCAGTG TGAAAAACTG GACCCACAAA
    GATTCAGAGA ACCTGGTCAA TGGGAACCAC TCTTCAGGAG AGGCAAAATC CCACTTGGTT
    TCCAACGAGA GAGTGCGCCC TGAGCACAGT GCCATCGTCA GTTCCATTGA GAAGGACCTT
    CAGGACATTA TGGACTCATT AGTCATGGAT GACCCTCAGC CCTCATCCTC CGAGTCAAAG
    AAGCCCTCTA GTCAGTCCAT CTCACAGTCT CCCCTTTCTC CGATGTTAAA TGGAGGGGGG
    CGCTTCCTTC TCTCTCCCCC AACGAGCCCT GGTGCTATGT CTGTGGGCTC CAGTTATGAG
    AATAACTCCC CTCCTTTCTC TCCTCTGTCA TCTCCCTCGG CTGCCAGCAG TGGCAGCTAC
    ACCACTCTCT CACCAATTGG TTGTCAGGAC CAGCCTCACA TGCTTCCTCC TGTGCCAGTG
    AGGTCTTCTA GTTACAATTA TACCAGCCAG CCACCCATAC CTCAGCCACG GACCATACTG
    CCCAGCTATT CCAGTGGCAG CAGTGGGCCA AAGGTGCCTG AGAGTCCCAG ACTCCAGCGA
    AAGGCGCTTT TAGAAGCACC GCCAAGCCCA AAGCCAAGCC GTAGGGGGTT GAACCAAGAC
    AGCTCGGTGG CTAAAACCCC AGACAGCCCC ATACAGACTC ACATTCTGCC CTCAGTCTCC
    ATCTCTACAG CTCCGACTGA TGTCCCATCT GTTAGTCGGG TACCAGTTCC AGGTAGTCCT
    AGATTAGCCA CCAAATTCTC CACTGCGTCC TCACCTGCCT CACCCTCTAG TCCCAGAACC
    AAAACTACCA TTGTCCTCCA AGATCGACCC TCAAGTCCTT TCCGGGAGCA ACCTCAGCCA
    GATCGTTCTT TGACGTCCAG CCCCTCTCAC CAACTCTCCC CACCATCAAG ATCTTTCCAG
    CCCCCATTGG ACCCCATTGT TCACATCATT CAAGGTGGAC CTTCACACCC ACATCCTCGA
    ACACTGCAGC CTCCAGAAAG TCCGCGGCTG GCCCGCAGAA ATCTTGAAGG GAGCAGCATG
    AGGGAACTCC CCCCTCTCAG TCCCTCCATA GCTCGCAGGG GGGTTCCTGT GCTCCCAGGT
    GCTCTACCAG GAACCCTGCG AACTCCAGAG AGCCCATCCC CAAGAATGGT GCCAGAGAGT
    CCCAGACTCA GGAGGAAAAC AGGGTCACCT ACAGAGGAGC CATTCAGTCC TCGTGCGATG
    CGCACTCGTA GTCCTTCGCC CACCTCTGGA CTGACTGGCG AAAGCAGTGG ACGGAAGGGA
    AGTTTTGGGA ACTCACTCTC TTCTGCCTTC AGCCTAAGTT CTCTGCCTGG GTCTTCCCCA
    CGATCTAGTC CCAGGAGCCA CCGAAAGATG TCAGCAGGCC ACAGAGACCT CCGGATGCCT
    CACCCAGGCA TGAGGGAGCG CAAAAACAGC ATCACAGAGA TCAGCGACAA CGAGGATGAC
    CTTCTGGAGT ACCATCGACG CCAAAGAGAA GAGCGACTCA GAGAGCAGGA AATGGAGAGA
    CTGGAACGGC AGCGGCTGGA GACAATCTTG AACCTCTGCG CTGAGTATAA CAAGGGCGAC
    GGGCCGCCGG GTGATTTGGG CAGGATGGGC GTCTCTGGGC TTGGCTCGAG GGACGGGCTT
    AACGTCAGGC GTCCTTCAAT GGACAGCGTG AACAGCGCTT CACTAAGAGC GACTCAAAGA
    CAACGGGAAA GCCAAGAGGA CAACCTGAAG GAGGATAGCA GTAGCACTGA GAGTGCGCAG
    CTGGACGGAC GGACACCTGC TCTGCTAAAT GGACAGAATG GACAGGCTGA GGAGTCTCTG
    GGTTCGGGCA GCATTGGCCG TCTGGAGCTG GGCTACCTGG AGGAGGAGCG GGTTCGAGTG
    CTGGCAAAGG TAGATGAGCT CAAAGCTCGA ATCACAGAAC TGGAGCAGCA ACTTCAGGAG
    TCTAAACAAG AGGCGGAGAT GGAGAGAGCT CTGCTGCAGG GAGAAAGACA GGCTGAGATG
    GAGCAAATCG AGACTGAGAC CGAGATTATC AACCAGCTGC AGCGCAAACT CACTGAACTG
    GAGAGCAGCA TCCAGAGAGA GAAGGACAAG GCATGCCATT CTTCCAGATG GCCACTCAAG
    GCCCATTCAG GTCTCGATTG CCATCCTCAG GAGAGGGCTA ATGTTGACGC GGAGCGGGAA
    GCCCTGGAGA GGCTTCAGGA TGGGTACAAT GAGTTGACGA GCCAGCTACA TAACTGCCCT
    GAGTCTCTGA GGGAACAGTT ACAGGAACAG CTCAAGAGGG AAGCTGAGAC TCTTGAAGCA
    GCGACAAAGC AGTTTGAAGA CCTGGAGTTT CAGCAGTTGG AGAGGGAGAG CAGTCTGGAG
    GAGGAGCGCG AGACCATCAG CCAGCAGCTC ATGCAGGACA GAGCTCAATA CCACAGCAGT
    GTCGCCAAGA GAAAGGAAAA GGTGGTGGCA TTGGAGAGCC AGGCGAGCCA GCTGGGAATA
    CAGGCCGCTC AGGAGTGTGA GAGACTGGCC AAGGACAGAA CCATCACCTT GCAGATGCTA
    CACAAGGAAA AGGAAAGGCT GGCCAGTCTG GAGAGAAGAT ACCAGAGTTT AACTGGTGGA
    AAGACGTTCC CCAAAAGCTC CAATACTATA AAGGAGGAGT ACTTGAAGCT GTCGGATGTG
    TATAAGATGT ATGGGAGTGA ATGCCATGAC ATCCAGCTCG CCAGCGCTTC CTCACACGGC
    CTCTCGCTTG CCCTTGACCC AGCTGAAACT GCCCCGCGTG AGGAGTATGT GACGGTCAGC
    CAATTAAACC AGATGTACGG GATGCCAAAG GTGGAGTCTT CCCCTACCTC ACCCGTCCAA
    TCAGTGCAGC CCTCTCTCGC AGACTCCACC TTCCCCCGCC TCCCTTCCCC TCATGGCTCC
    TCCCTCTCTC TCTCCTTCGA GCGTCAGTCA GACTGGGGCA GGCCTCAGAT GCCCGTGCTA
    GATTTAGAGC ACTGGTACCA GGAGGTAATG GCAGCTGGAG ATGTAAACCA GCTCTGCCCT
    CCTCCATTGC CGGCTAAAGC TTTCTCATCA TCATCATCAT CACCACGCAG GCCTGTGCAG
    GTGTACAGAT CTCGACTGGA CAGTGATTGC AGCCAATCCT CACTTAGACC GAAAGTCAGC
    GCCGGTTTGT CTCCTACATA CACGACGGCC ACGCTGGGAC GAAACAGTCC TGCCAGGAGT
    CCTCTGATGG TGGCCAACAG CACCGGAAGT CTCCCACGCA ACCTGGCAGC CACCCTGCAG
    GACATCGAGA CCAAGAGACA GCTGGCTCTT CAGCAGAAAG GTCAGCAGGT TATCGAGGAG
    CAGCGCAGGC GTCTGGCGGA GCTGAAGCAG AAAGCTGCAG TGGAGGCTCA GTGCCAGTGG
    GAGGCGCTGC ACGGCTCTCA AACGCAGCTC CACCAGCCCT TCTCATCAAG CCCAGCACCG
    GCGCTTCACC ACTCCATCCT GCACCACACA GCGCCCCCTT GCGGAGAGCA GCCGTACGAC
    ACGCTAAGCC TGGAGAGCTC GGACAGCATG GACACCAGCA TCTCTACTGG AAACAACTCG
    GCCTGCTCGC CGGACAACAT GTCCAGTGCC AGCGGTATGG ATGCGTTGAA GATTGAAGAG
    ATGGAGAAGA TGTTGAAGGA GGCGCAGCTG GAAAAAGCTC GGCTCATAGA GTCTAGAGAG
    CGAGAGACTC AGAGCCGTAA ACTGATGCTG GAGGAGGAGC GGAGGAGGAG AGAGGAGGCC
    GAGAAGAGGC TGCAGGAGGA GACGCTGCAC AGACAGCAGC TCATCGAGAA GGAGGTCAAG
    ATGAGGGCCA GAAACTTCTC TCAGGCCCGT CCGATGACCC GCTACCTGCC CATCCGCAAA
    GAGGAGTTTG ACCTGCGTTC GCACATCGAG TCTTCAGGCC ACAGCGTAGA CACCTGCTAC
    CACGTCATCC TCACCGAGAA GATGTGCAAG GGCTTTCTGG TCAAGATGGG AGGCAAGATC
    AAATCCTGGA AGAAGCGCTG GTTCGTCTTT GATCGCCTCA AAAGGACGTT CTCTTACTAC
    GCCGACAAAC ACGAGAGCAA GCTGAAGGGT GTGATTTACT TCCAGGCCAT CGAGGAGGTT
    TACTACGATC ACCTGCGCAG CGCCACAAAG AAAGGATTTT TCAATCTGAA TCTGACAAAC
    AGCCCGACCC CGACGCTGAC CTTCTGTGTT AAAACACACG ACAGACTGTA TTACATGGTG
    GCTCCGTCCG CAGAGGCCAT GAGGATATGG ATGGATGTGA TTGTGACAGG AGCCGAGGGC
    TACACACAGT TCATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322044.1
    CDS617..5017
    Translation

    Target ORF information:

    RefSeq Version XM_021466369.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCTGTGTT TCGGCCAATC CGCCTTCTTC CGCTTCAACC ACCCCGAGGA GGCTTTCCGA 
    ATGAAAAGCA TGATGCCCGA GGGAGGACAG ACAGCCAGTG TGAAAAACTG GACCCACAAA
    GATTCAGAGA ACCTGGTCAA TGGGAACCAC TCTTCAGGAG AGGCAAAATC CCACTTGGTT
    TCCAACGAGA GAGTGCGCCC TGAGCACAGT GCCATCGTCA GTTCCATTGA GAAGGACCTT
    CAGGACATTA TGGACTCATT AGTCATGGAT GACCCTCAGC CCTCATCCTC CGAGTCAAAG
    AAGCCCTCTA GTCAGTCCAT CTCACAGTCT CCCCTTTCTC CGATGTTAAA TGGAGGGGGG
    CGCTTCCTTC TCTCTCCCCC AACGAGCCCT GGTGCTATGT CTGTGGGCTC CAGTTATGAG
    AATAACTCCC CTCCTTTCTC TCCTCTGTCA TCTCCCTCGG CTGCCAGCAG TGGCAGCTAC
    ACCACTCTCT CACCAATTGG TTGTCAGGAC CAGCCTCACA TGCTTCCTCC TGTGCCAGTG
    AGGTCTTCTA GTTACAATTA TACCAGCCAG CCACCCATAC CTCAGCCACG GACCATACTG
    CCCAGCTATT CCAGTGGCAG CAGTGGGCCA AAGGTGCCTG AGAGTCCCAG ACTCCAGCGA
    AAGGCGCTTT TAGAAGCACC GCCAAGCCCA AAGCCAAGCC GTAGGGGGTT GAACCAAGAC
    AGCTCGGTGG CTAAAACCCC AGACAGCCCC ATACAGACTC ACATTCTGCC CTCAGTCTCC
    ATCTCTACAG CTCCGACTGA TGTCCCATCT GTTAGTCGGG TACCAGTTCC AGGTAGTCCT
    AGATTAGCCA CCAAATTCTC CACTGCGTCC TCACCTGCCT CACCCTCTAG TCCCAGAACC
    AAAACTACCA TTGTCCTCCA AGATCGACCC TCAAGTCCTT TCCGGGAGCA ACCTCAGCCA
    GATCGTTCTT TGACGTCCAG CCCCTCTCAC CAACTCTCCC CACCATCAAG ATCTTTCCAG
    CCCCCATTGG ACCCCATTGT TCACATCATT CAAGGTGGAC CTTCACACCC ACATCCTCGA
    ACACTGCAGC CTCCAGAAAG TCCGCGGCTG GCCCGCAGAA ATCTTGAAGG GAGCAGCATG
    AGGGAACTCC CCCCTCTCAG TCCCTCCATA GCTCGCAGGG GGGTTCCTGT GCTCCCAGGT
    GCTCTACCAG GAACCCTGCG AACTCCAGAG AGCCCATCCC CAAGAATGGT GCCAGAGAGT
    CCCAGACTCA GGAGGAAAAC AGGGTCACCT ACAGAGGAGC CATTCAGTCC TCGTGCGATG
    CGCACTCGTA GTCCTTCGCC CACCTCTGGA CTGACTGGCG AAAGCAGTGG ACGGAAGGGA
    AGTTTTGGGA ACTCACTCTC TTCTGCCTTC AGCCTAAGTT CTCTGCCTGG GTCTTCCCCA
    CGATCTAGTC CCAGGAGCCA CCGAAAGATG TCAGCAGGCC ACAGAGACCT CCGGATGCCT
    CACCCAGGCA TGAGGGAGCG CAAAAACAGC ATCACAGAGA TCAGCGACAA CGAGGATGAC
    CTTCTGGAGT ACCATCGACG CCAAAGAGAA GAGCGACTCA GAGAGCAGGA AATGGAGAGA
    CTGGAACGGC AGCGGCTGGA GACAATCTTG AACCTCTGCG CTGAGTATAA CAAGGGCGAC
    GGGCCGCCGG GTGATTTGGG CAGGATGGGC GTCTCTGGGC TTGGCTCGAG GGACGGGCTT
    AACGTCAGGC GTCCTTCAAT GGACAGCGTG AACAGCGCTT CACTAAGAGC GACTCAAAGA
    CAACGGGAAA GCCAAGAGGA CAACCTGAAG GAGGATAGCA GTAGCACTGA GAGTGCGCAG
    CTGGACGGAC GGACACCTGC TCTGCTAAAT GGACAGAATG GACAGGCTGA GGAGTCTCTG
    GGTTCGGGCA GCATTGGCCG TCTGGAGCTG GGCTACCTGG AGGAGGAGCG GGTTCGAGTG
    CTGGCAAAGG TAGATGAGCT CAAAGCTCGA ATCACAGAAC TGGAGCAGCA ACTTCAGGAG
    TCTAAACAAG AGGCGGAGAT GGAGAGAGCT CTGCTGCAGG GAGAAAGACA GGCTGAGATG
    GAGCAAATCG AGACTGAGAC CGAGATTATC AACCAGCTGC AGCGCAAACT CACTGAACTG
    GAGAGCAGCA TCCAGAGAGA GAAGGACAAG GCATGCCATT CTTCCAGATG GCCACTCAAG
    GCCCATTCAG GTCTCGATTG CCATCCTCAG GAGAGGGCTA ATGTTGACGC GGAGCGGGAA
    GCCCTGGAGA GGCTTCAGGA TGGGTACAAT GAGTTGACGA GCCAGCTACA TAACTGCCCT
    GAGTCTCTGA GGGAACAGTT ACAGGAACAG CTCAAGAGGG AAGCTGAGAC TCTTGAAGCA
    GCGACAAAGC AGTTTGAAGA CCTGGAGTTT CAGCAGTTGG AGAGGGAGAG CAGTCTGGAG
    GAGGAGCGCG AGACCATCAG CCAGCAGCTC ATGCAGGACA GAGCTCAATA CCACAGCAGT
    GTCGCCAAGA GAAAGGAAAA GGTGGTGGCA TTGGAGAGCC AGGCGAGCCA GCTGGGAATA
    CAGGCCGCTC AGGAGTGTGA GAGACTGGCC AAGGACAGAA CCATCACCTT GCAGATGCTA
    CACAAGGAAA AGGAAAGGCT GGCCAGTCTG GAGAGAAGAT ACCAGAGTTT AACTGGTGGA
    AAGACGTTCC CCAAAAGCTC CAATACTATA AAGGAGGAGT ACTTGAAGCT GTCGGATGTG
    TATAAGATGT ATGGGAGTGA ATGCCATGAC ATCCAGCTCG CCAGCGCTTC CTCACACGGC
    CTCTCGCTTG CCCTTGACCC AGCTGAAACT GCCCCGCGTG AGGAGTATGT GACGGTCAGC
    CAATTAAACC AGATGTACGG GATGCCAAAG GTGGAGTCTT CCCCTACCTC ACCCGTCCAA
    TCAGTGCAGC CCTCTCTCGC AGACTCCACC TTCCCCCGCC TCCCTTCCCC TCATGGCTCC
    TCCCTCTCTC TCTCCTTCGA GCGTCAGTCA GACTGGGGCA GGCCTCAGAT GCCCGTGCTA
    GATTTAGAGC ACTGGTACCA GGAGGTAATG GCAGCTGGAG ATGTAAACCA GCTCTGCCCT
    CCTCCATTGC CGGCTAAAGC TTTCTCATCA TCATCATCAT CACCACGCAG GCCTGTGCAG
    GTGTACAGAT CTCGACTGGA CAGTGATTGC AGCCAATCCT CACTTAGACC GAAAGTCAGC
    GCCGGTTTGT CTCCTACATA CACGACGGCC ACGCTGGGAC GAAACAGTCC TGCCAGGAGT
    CCTCTGATGG TGGCCAACAG CACCGGAAGT CTCCCACGCA ACCTGGCAGC CACCCTGCAG
    GACATCGAGA CCAAGAGACA GCTGGCTCTT CAGCAGAAAG GTCAGCAGGT TATCGAGGAG
    CAGCGCAGGC GTCTGGCGGA GCTGAAGCAG AAAGCTGCAG TGGAGGCTCA GTGCCAGTGG
    GAGGCGCTGC ACGGCTCTCA AACGCAGCTC CACCAGCCCT TCTCATCAAG CCCAGCACCG
    GCGCTTCACC ACTCCATCCT GCACCACACA GCGCCCCCTT GCGGAGAGCA GCCGTACGAC
    ACGCTAAGCC TGGAGAGCTC GGACAGCATG GACACCAGCA TCTCTACTGG AAACAACTCG
    GCCTGCTCGC CGGACAACAT GTCCAGTGCC AGCGGTATGG ATGCGTTGAA GATTGAAGAG
    ATGGAGAAGA TGTTGAAGGA GGCGCAGCTG GAAAAAGCTC GGCTCATAGA GTCTAGAGAG
    CGAGAGACTC AGAGCCGTAA ACTGATGCTG GAGGAGGAGC GGAGGAGGAG AGAGGAGGCC
    GAGAAGAGGC TGCAGGAGGA GACGCTGCAC AGACAGCAGC TCATCGAGAA GGAGGTCAAG
    ATGAGGGCCA GAAACTTCTC TCAGGCCCGT CCGATGACCC GCTACCTGCC CATCCGCAAA
    GAGGAGTTTG ACCTGCGTTC GCACATCGAG TCTTCAGGCC ACAGCGTAGA CACCTGCTAC
    CACGTCATCC TCACCGAGAA GATGTGCAAG GGCTTTCTGG TCAAGATGGG AGGCAAGATC
    AAATCCTGGA AGAAGCGCTG GTTCGTCTTT GATCGCCTCA AAAGGACGTT CTCTTACTAC
    GCCGACAAAC ACGAGAGCAA GCTGAAGGGT GTGATTTACT TCCAGGCCAT CGAGGAGGTT
    TACTACGATC ACCTGCGCAG CGCCACAAAG AAAGGATTTT TCAATCTGAA TCTGACAAAC
    AGCCCGACCC CGACGCTGAC CTTCTGTGTT AAAACACACG ACAGACTGTA TTACATGGTG
    GCTCCGTCCG CAGAGGCCAT GAGGATATGG ATGGATGTGA TTGTGACAGG AGCCGAGGGC
    TACACACAGT TCATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55244
    Clone ID Related Accession (Same CDS sequence) XM_017351158.1
    Accession Version XM_017351158.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4182bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTGTGTT TCGGCCAATC CGCCTTCTTC CGCTTCAACC ACCCCGAGGA GGCTTTCCGA 
    ATGAAAAGCA TGATGCCCGA GGGAGGACAG ACAGCCAGTG TGAAAAACTG GACCCACAAA
    GATTCAGAGA ACCTGGTCAA TGGGAACCAC TCTTCAGGAG AGGCAAAATC CCACTTGGTT
    TCCAACGAGA GAGTGCGCCC TGAGCACAGT GCCATCGTCA GTTCCATTGA GAAGGACCTT
    CAGGACATTA TGGACTCATT AGTCATGGAT GACCCTCAGC CCTCATCCTC CGAGTCAAAG
    AAGCCCTCTA GTCAGTCCAT CTCACAGTCT CCCCTTTCTC CGATGTTAAA TGGAGGGGGG
    CGCTTCCTTC TCTCTCCCCC AACGAGCCCT GGTGCTATGT CTGTGGGCTC CAGTTATGAG
    AATAACTCCC CTCCTTTCTC TCCTCTGTCA TCTCCCTCGG CTGCCAGCAG TGGCAGCTAC
    ACCACTCTCT CACCAATTGG TTGTCAGGAC CAGCCTCACA TGCTTCCTCC TGTGCCAGTG
    AGGTCTTCTA GTTACAATTA TACCAGCCAG CCACCCATAC CTCAGCCACG GACCATACTG
    CCCAGCTATT CCAGTGGCAG CAGTGGGCCA AAGGTGCCTG AGAGTCCCAG ACTCCAGCGA
    AAGGCGCTTT TAGAAGCACC GCCAAGCCCA AAGCCAAGCC GTAGGGGGTT GAACCAAGAC
    AGCTCGGTGG CTAAAACCCC AGACAGCCCC ATACAGACTC ACATTCTGCC CTCAGTCTCC
    ATCTCTACAG CTCCGACTGA TGTCCCATCT GTTAGTCGGG TACCAGTTCC AGGTAGTCCT
    AGATTAGCCA CCAAATTCTC CACTGCGTCC TCACCTGCCT CACCCTCTAG TCCCAGAACC
    AAAACTACCA TTGTCCTCCA AGATCGACCC TCAAGTCCTT TCCGGGAGCA ACCTCAGCCA
    GATCGTTCTT TGACGTCCAG CCCCTCTCAC CAACTCTCCC CACCATCAAG ATCTTTCCAG
    CCCCCATTGG ACCCCATTGT TCACATCATT CAAGGTGGAC CTTCACACCC ACATCCTCGA
    ACACTGCAGC CTCCAGAAAG TCCGCGGCTG GCCCGCAGAA ATCTTGAAGG GAGCAGCATG
    AGGGAACTCC CCCCTCTCAG TCCCTCCATA GCTCGCAGGG GGGTTCCTGT GCTCCCAGGT
    GCTCTACCAG GAACCCTGCG AACTCCAGAG AGCCCATCCC CAAGAATGGT GCCAGAGAGT
    CCCAGACTCA GGAGGAAAAC AGGGTCACCT ACAGAGGAGC CATTCAGTCC TCGTGCGATG
    CGCACTCGTA GTCCTTCGCC CACCTCTGGA CTGACTGGCG AAAGCAGTGG ACGGAAGGGA
    AGTTTTGGGA ACTCACTCTC TTCTGCCTTC AGCCTAAGTT CTCTGCCTGG GTCTTCCCCA
    CGATCTAGTC CCAGGAGCCA CCGAAAGATG TCAGCAGGCC ACAGAGACCT CCGGATGCCT
    CACCCAGGCA TGAGGGAGCG CAAAAACAGC ATCACAGAGA TCAGCGACAA CGAGGATGAC
    CTTCTGGAGT ACCATCGACG CCAAAGAGAA GAGCGACTCA GAGAGCAGGA AATGGAGAGA
    CTGGAACGGC AGCGGCTGGA GACAATCTTG AACCTCTGCG CTGAGTATAA CAAGGGCGAC
    GGGCCGCCGG GTGATTTGGG CAGGATGGGC GTCTCTGGGC TTGGCTCGAG GGACGGGCTT
    AACGTCAGGC GTCCTTCAAT GGACAGCGTG AACAGCGCTT CACTAAGAGC GACTCAAAGA
    CAACGGGAAA GCCAAGAGGA CAACCTGAAG GAGGATAGCA GTAGCACTGA GAGTGCGCAG
    CTGGACGGAC GGACACCTGC TCTGCTAAAT GGACAGAATG GACAGGCTGA GGAGTCTCTG
    GGTTCGGGCA GCATTGGCCG TCTGGAGCTG GGCTACCTGG AGGAGGAGCG GGTTCGAGTG
    CTGGCAAAGG TAGATGAGCT CAAAGCTCGA ATCACAGAAC TGGAGCAGCA ACTTCAGGAG
    TCTAAACAAG AGGCGGAGAT GGAGAGAGCT CTGCTGCAGG GAGAAAGACA GGCTGAGATG
    GAGCAAATCG AGACTGAGAC CGAGATTATC AACCAGCTGC AGCGCAAACT CACTGAACTG
    GAGAGCAGCA TCCAGAGAGA GAAGGACAAG GAGAGGGCTA ATGTTGACGC GGAGCGGGAA
    GCCCTGGAGA GGCTTCAGGA TGGGTACAAT GAGTTGACGA GCCAGCTACA TAACTGCCCT
    GAGTCTCTGA GGGAACAGTT ACAGGAACAG CTCAAGAGGG AAGCTGAGAC TCTTGAAGCA
    GCGACAAAGC AGTTTGAAGA CCTGGAGTTT CAGCAGTTGG AGAGGGAGAG CAGTCTGGAG
    GAGGAGCGCG AGACCATCAG CCAGCAGCTC ATGCAGGACA GAGCTCAATA CCACAGCAGT
    GTCGCCAAGA GAAAGGAAAA GGTGGTGGCA TTGGAGAGCC AGGCGAGCCA GCTGGGAATA
    CAGGCCGCTC AGGAGTGTGA GAGACTGGCC AAGGACAGAA CCATCACCTT GCAGATGCTA
    CACAAGGAAA AGGAAAGGCT GGCCAGTCTG GAGAGAAGAT ACCAGAGTTT AACTGGTGGA
    AAGACGTTCC CCAAAAGCTC CAATACTATA AAGGAGGAGT ACTTGAAGCT GTCGGATGTG
    TATAAGATGT ATGGGAGTGA ATGCCATGAC ATCCAGCTCG CCAGCGCTTC CTCACACGGC
    CTCTCGCTTG CCCTTGACCC AGCTGAAACT GCCCCGCGTG AGGAGTATGT GACGGTCAGC
    CAATTAAACC AGATGTACGG GATGCCAAAG GTGGAGTCTT CCCCTACCTC ACCCGTCCAA
    TCAGTGCAGC CCTCTCTCGC AGACTCCACC TTCCCCCGCC TCCCTTCCCC TCATGGCTCC
    TCCCTCTCTC TCTCCTTCGA GGTGTACAGA TCTCGACTGG ACAGTGATTG CAGCCAATCC
    TCACTTAGAC CGAAAGTCAG CGCCGGTTTG TCTCCTACAT ACACGACGGC CACGCTGGGA
    CGAAACAGTC CTGCCAGGAG TCCTCTGATG GTGGCCAACA GCACCGGAAG TCTCCCACGC
    AACCTGGCAG CCACCCTGCA GGACATCGAG ACCAAGAGAC AGCTGGCTCT TCAGCAGAAA
    GGTCAGCAGG TTATCGAGGA GCAGCGCAGG CGTCTGGCGG AGCTGAAGCA GAAAGCTGCA
    GTGGAGGCTC AGTGCCAGTG GGAGGCGCTG CACGGCTCTC AAACGCAGCT CCACCAGCCC
    TTCTCATCAA GCCCAGCACC GGCGCTTCAC CACTCCATCC TGCACCACAC AGCGCCCCCT
    TGCGGAGAGC AGCCGTACGA CACGCTAAGC CTGGAGAGCT CGGACAGCAT GGACACCAGC
    ATCTCTACTG GAAACAACTC GGCCTGCTCG CCGGACAACA TGTCCAGTGC CAGCGGTATG
    GATGCGTTGA AGATTGAAGA GATGGAGAAG ATGTTGAAGG AGGCGCAGCT GGAAAAAGCT
    CGGCTCATAG AGTCTAGAGA GCGAGAGACT CAGAGCCGTA AACTGATGCT GGAGGAGGAG
    CGGAGGAGGA GAGAGGAGGC CGAGAAGAGG CTGCAGGAGG AGACGCTGCA CAGACAGCAG
    CTCATCGAGA AGGAGGTCAA GATGAGGGCC AGAAACTTCT CTCAGGCCCG TCCGATGACC
    CGCTACCTGC CCATCCGCAA AGAGGAGTTT GACCTGCGTT CGCACATCGA GTCTTCAGGC
    CACAGCGTAG ACACCTGCTA CCACGTCATC CTCACCGAGA AGATGTGCAA GGGCTTTCTG
    GTCAAGATGG GAGGCAAGAT CAAATCCTGG AAGAAGCGCT GGTTCGTCTT TGATCGCCTC
    AAAAGGACGT TCTCTTACTA CGCCGACAAA CACGAGAGCA AGCTGAAGGG TGTGATTTAC
    TTCCAGGCCA TCGAGGAGGT TTACTACGAT CACCTGCGCA GCGCCACAAA GAAAGGATTT
    TTCAATCTGA ATCTGACAAA CAGCCCGACC CCGACGCTGA CCTTCTGTGT TAAAACACAC
    GACAGACTGT ATTACATGGT GGCTCCGTCC GCAGAGGCCA TGAGGATATG GATGGATGTG
    ATTGTGACAG GAGCCGAGGG CTACACACAG TTCATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206647.1
    CDS598..4779
    Translation

    Target ORF information:

    RefSeq Version XM_017351158.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351158.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCTGTGTT TCGGCCAATC CGCCTTCTTC CGCTTCAACC ACCCCGAGGA GGCTTTCCGA 
    ATGAAAAGCA TGATGCCCGA GGGAGGACAG ACAGCCAGTG TGAAAAACTG GACCCACAAA
    GATTCAGAGA ACCTGGTCAA TGGGAACCAC TCTTCAGGAG AGGCAAAATC CCACTTGGTT
    TCCAACGAGA GAGTGCGCCC TGAGCACAGT GCCATCGTCA GTTCCATTGA GAAGGACCTT
    CAGGACATTA TGGACTCATT AGTCATGGAT GACCCTCAGC CCTCATCCTC CGAGTCAAAG
    AAGCCCTCTA GTCAGTCCAT CTCACAGTCT CCCCTTTCTC CGATGTTAAA TGGAGGGGGG
    CGCTTCCTTC TCTCTCCCCC AACGAGCCCT GGTGCTATGT CTGTGGGCTC CAGTTATGAG
    AATAACTCCC CTCCTTTCTC TCCTCTGTCA TCTCCCTCGG CTGCCAGCAG TGGCAGCTAC
    ACCACTCTCT CACCAATTGG TTGTCAGGAC CAGCCTCACA TGCTTCCTCC TGTGCCAGTG
    AGGTCTTCTA GTTACAATTA TACCAGCCAG CCACCCATAC CTCAGCCACG GACCATACTG
    CCCAGCTATT CCAGTGGCAG CAGTGGGCCA AAGGTGCCTG AGAGTCCCAG ACTCCAGCGA
    AAGGCGCTTT TAGAAGCACC GCCAAGCCCA AAGCCAAGCC GTAGGGGGTT GAACCAAGAC
    AGCTCGGTGG CTAAAACCCC AGACAGCCCC ATACAGACTC ACATTCTGCC CTCAGTCTCC
    ATCTCTACAG CTCCGACTGA TGTCCCATCT GTTAGTCGGG TACCAGTTCC AGGTAGTCCT
    AGATTAGCCA CCAAATTCTC CACTGCGTCC TCACCTGCCT CACCCTCTAG TCCCAGAACC
    AAAACTACCA TTGTCCTCCA AGATCGACCC TCAAGTCCTT TCCGGGAGCA ACCTCAGCCA
    GATCGTTCTT TGACGTCCAG CCCCTCTCAC CAACTCTCCC CACCATCAAG ATCTTTCCAG
    CCCCCATTGG ACCCCATTGT TCACATCATT CAAGGTGGAC CTTCACACCC ACATCCTCGA
    ACACTGCAGC CTCCAGAAAG TCCGCGGCTG GCCCGCAGAA ATCTTGAAGG GAGCAGCATG
    AGGGAACTCC CCCCTCTCAG TCCCTCCATA GCTCGCAGGG GGGTTCCTGT GCTCCCAGGT
    GCTCTACCAG GAACCCTGCG AACTCCAGAG AGCCCATCCC CAAGAATGGT GCCAGAGAGT
    CCCAGACTCA GGAGGAAAAC AGGGTCACCT ACAGAGGAGC CATTCAGTCC TCGTGCGATG
    CGCACTCGTA GTCCTTCGCC CACCTCTGGA CTGACTGGCG AAAGCAGTGG ACGGAAGGGA
    AGTTTTGGGA ACTCACTCTC TTCTGCCTTC AGCCTAAGTT CTCTGCCTGG GTCTTCCCCA
    CGATCTAGTC CCAGGAGCCA CCGAAAGATG TCAGCAGGCC ACAGAGACCT CCGGATGCCT
    CACCCAGGCA TGAGGGAGCG CAAAAACAGC ATCACAGAGA TCAGCGACAA CGAGGATGAC
    CTTCTGGAGT ACCATCGACG CCAAAGAGAA GAGCGACTCA GAGAGCAGGA AATGGAGAGA
    CTGGAACGGC AGCGGCTGGA GACAATCTTG AACCTCTGCG CTGAGTATAA CAAGGGCGAC
    GGGCCGCCGG GTGATTTGGG CAGGATGGGC GTCTCTGGGC TTGGCTCGAG GGACGGGCTT
    AACGTCAGGC GTCCTTCAAT GGACAGCGTG AACAGCGCTT CACTAAGAGC GACTCAAAGA
    CAACGGGAAA GCCAAGAGGA CAACCTGAAG GAGGATAGCA GTAGCACTGA GAGTGCGCAG
    CTGGACGGAC GGACACCTGC TCTGCTAAAT GGACAGAATG GACAGGCTGA GGAGTCTCTG
    GGTTCGGGCA GCATTGGCCG TCTGGAGCTG GGCTACCTGG AGGAGGAGCG GGTTCGAGTG
    CTGGCAAAGG TAGATGAGCT CAAAGCTCGA ATCACAGAAC TGGAGCAGCA ACTTCAGGAG
    TCTAAACAAG AGGCGGAGAT GGAGAGAGCT CTGCTGCAGG GAGAAAGACA GGCTGAGATG
    GAGCAAATCG AGACTGAGAC CGAGATTATC AACCAGCTGC AGCGCAAACT CACTGAACTG
    GAGAGCAGCA TCCAGAGAGA GAAGGACAAG GAGAGGGCTA ATGTTGACGC GGAGCGGGAA
    GCCCTGGAGA GGCTTCAGGA TGGGTACAAT GAGTTGACGA GCCAGCTACA TAACTGCCCT
    GAGTCTCTGA GGGAACAGTT ACAGGAACAG CTCAAGAGGG AAGCTGAGAC TCTTGAAGCA
    GCGACAAAGC AGTTTGAAGA CCTGGAGTTT CAGCAGTTGG AGAGGGAGAG CAGTCTGGAG
    GAGGAGCGCG AGACCATCAG CCAGCAGCTC ATGCAGGACA GAGCTCAATA CCACAGCAGT
    GTCGCCAAGA GAAAGGAAAA GGTGGTGGCA TTGGAGAGCC AGGCGAGCCA GCTGGGAATA
    CAGGCCGCTC AGGAGTGTGA GAGACTGGCC AAGGACAGAA CCATCACCTT GCAGATGCTA
    CACAAGGAAA AGGAAAGGCT GGCCAGTCTG GAGAGAAGAT ACCAGAGTTT AACTGGTGGA
    AAGACGTTCC CCAAAAGCTC CAATACTATA AAGGAGGAGT ACTTGAAGCT GTCGGATGTG
    TATAAGATGT ATGGGAGTGA ATGCCATGAC ATCCAGCTCG CCAGCGCTTC CTCACACGGC
    CTCTCGCTTG CCCTTGACCC AGCTGAAACT GCCCCGCGTG AGGAGTATGT GACGGTCAGC
    CAATTAAACC AGATGTACGG GATGCCAAAG GTGGAGTCTT CCCCTACCTC ACCCGTCCAA
    TCAGTGCAGC CCTCTCTCGC AGACTCCACC TTCCCCCGCC TCCCTTCCCC TCATGGCTCC
    TCCCTCTCTC TCTCCTTCGA GGTGTACAGA TCTCGACTGG ACAGTGATTG CAGCCAATCC
    TCACTTAGAC CGAAAGTCAG CGCCGGTTTG TCTCCTACAT ACACGACGGC CACGCTGGGA
    CGAAACAGTC CTGCCAGGAG TCCTCTGATG GTGGCCAACA GCACCGGAAG TCTCCCACGC
    AACCTGGCAG CCACCCTGCA GGACATCGAG ACCAAGAGAC AGCTGGCTCT TCAGCAGAAA
    GGTCAGCAGG TTATCGAGGA GCAGCGCAGG CGTCTGGCGG AGCTGAAGCA GAAAGCTGCA
    GTGGAGGCTC AGTGCCAGTG GGAGGCGCTG CACGGCTCTC AAACGCAGCT CCACCAGCCC
    TTCTCATCAA GCCCAGCACC GGCGCTTCAC CACTCCATCC TGCACCACAC AGCGCCCCCT
    TGCGGAGAGC AGCCGTACGA CACGCTAAGC CTGGAGAGCT CGGACAGCAT GGACACCAGC
    ATCTCTACTG GAAACAACTC GGCCTGCTCG CCGGACAACA TGTCCAGTGC CAGCGGTATG
    GATGCGTTGA AGATTGAAGA GATGGAGAAG ATGTTGAAGG AGGCGCAGCT GGAAAAAGCT
    CGGCTCATAG AGTCTAGAGA GCGAGAGACT CAGAGCCGTA AACTGATGCT GGAGGAGGAG
    CGGAGGAGGA GAGAGGAGGC CGAGAAGAGG CTGCAGGAGG AGACGCTGCA CAGACAGCAG
    CTCATCGAGA AGGAGGTCAA GATGAGGGCC AGAAACTTCT CTCAGGCCCG TCCGATGACC
    CGCTACCTGC CCATCCGCAA AGAGGAGTTT GACCTGCGTT CGCACATCGA GTCTTCAGGC
    CACAGCGTAG ACACCTGCTA CCACGTCATC CTCACCGAGA AGATGTGCAA GGGCTTTCTG
    GTCAAGATGG GAGGCAAGAT CAAATCCTGG AAGAAGCGCT GGTTCGTCTT TGATCGCCTC
    AAAAGGACGT TCTCTTACTA CGCCGACAAA CACGAGAGCA AGCTGAAGGG TGTGATTTAC
    TTCCAGGCCA TCGAGGAGGT TTACTACGAT CACCTGCGCA GCGCCACAAA GAAAGGATTT
    TTCAATCTGA ATCTGACAAA CAGCCCGACC CCGACGCTGA CCTTCTGTGT TAAAACACAC
    GACAGACTGT ATTACATGGT GGCTCCGTCC GCAGAGGCCA TGAGGATATG GATGGATGTG
    ATTGTGACAG GAGCCGAGGG CTACACACAG TTCATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149045
    Clone ID Related Accession (Same CDS sequence) XM_021466360.1
    Accession Version XM_021466360.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4854bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGGAGCC ATGTTGAGGG TCTTGTTTCA TTGGCTGCCA GAAAGTTATT GCAGGATTCT 
    CATGTCCCCG AGCAAGTAAG TGACAGTACA CTGGCCATGG ATACTTTGAA CCAAAATCTT
    AGCCATGGGC ATCAGTCTCA TCAGGTGCTC CAGAGTACTC CACTGGATCT TATTGAGACT
    GGAAAGGCAC TGAAGGTTCA AGCAGAGCGC CCCCATTTAG TCAGTCTGGG AAGTGGGCGT
    CTCAGTACAG CCATCACCCT CTTACCCCTG CCTGAAGGGA AGACGACTGT GGGTCATGGA
    GCCATGGACA TCAGCATACA GGGCCCCGGG GTCGCAGCTC AGCACTGCTT TATTGAGAAC
    AGGGCTGGAA TCATCACACT TCACCCCTGT GGAAACCAGT GCAGCATGGA CGGACTGCCG
    GTCAACAAAC CTGTGCGACT CTCTCAAGGC TGCATGCTGT GTTTCGGCCA ATCCGCCTTC
    TTCCGCTTCA ACCACCCCGA GGAGGCTTTC CGAATGAAAA GCATGATGCC CGAGGGAGGA
    CAGACAGCCA GTGTGAAAAA CTGGACCCAC AAAGATTCAG AGAACCTGGT CAATGGGAAC
    CACTCTTCAG GAGAGGCAAA ATCCCACTTG GTTTCCAACG AGAGAGTGCG CCCTGAGCAC
    AGTGCCATCG TCAGTTCCAT TGAGAAGGAC CTTCAGGACA TTATGGACTC ATTAGTCATG
    GATGACCCTC AGCCCTCATC CTCCGAGTCA AAGAAGCCCT CTAGTCAGTC CATCTCACAG
    TCTCCCCTTT CTCCGATGTT AAATGGAGGG GGGCGCTTCC TTCTCTCTCC CCCAACGAGC
    CCTGGTGCTA TGTCTGTGGG CTCCAGTTAT GAGAATAACT CCCCTCCTTT CTCTCCTCTG
    TCATCTCCCT CGGCTGCCAG CAGTGGCAGC TACACCACTC TCTCACCAAT TGGTTGTCAG
    GACCAGCCTC ACATGCTTCC TCCTGTGCCA GTGAGGTCTT CTAGTTACAA TTATACCAGC
    CAGCCACCCA TACCTCAGCC ACGGACCATA CTGCCCAGCT ATTCCAGTGG CAGCAGTGGG
    CCAAAGGTGC CTGAGAGTCC CAGACTCCAG CGAAAGGCGC TTTTAGAAGC ACCGCCAAGC
    CCAAAGCCAA GCCGTAGGGG GTTGAACCAA GACAGCTCGG TGGCTAAAAC CCCAGACAGC
    CCCATACAGA CTCACATTCT GCCCTCAGTC TCCATCTCTA CAGCTCCGAC TGATGTCCCA
    TCTGTTAGTC GGGTACCAGT TCCAGGTAGT CCTAGATTAG CCACCAAATT CTCCACTGCG
    TCCTCACCTG CCTCACCCTC TAGTCCCAGA ACCAAAACTA CCATTGTCCT CCAAGATCGA
    CCCTCAAGTC CTTTCCGGGA GCAACCTCAG CCAGATCGTT CTTTGACGTC CAGCCCCTCT
    CACCAACTCT CCCCACCATC AAGATCTTTC CAGCCCCCAT TGGACCCCAT TGTTCACATC
    ATTCAAGGTG GACCTTCACA CCCACATCCT CGAACACTGC AGCCTCCAGA AAGTCCGCGG
    CTGGCCCGCA GAAATCTTGA AGGGAGCAGC ATGAGGGAAC TCCCCCCTCT CAGTCCCTCC
    ATAGCTCGCA GGGGGGTTCC TGTGCTCCCA GGTGCTCTAC CAGGAACCCT GCGAACTCCA
    GAGAGCCCAT CCCCAAGAAT GGTGCCAGAG AGTCCCAGAC TCAGGAGGAA AACAGGGTCA
    CCTACAGAGG AGCCATTCAG TCCTCGTGCG ATGCGCACTC GTAGTCCTTC GCCCACCTCT
    GGACTGACTG GCGAAAGCAG TGGACGGAAG GGAAGTTTTG GGAACTCACT CTCTTCTGCC
    TTCAGCCTAA GTTCTCTGCC TGGGTCTTCC CCACGATCTA GTCCCAGGAG CCACCGAAAG
    ATGTCAGCAG GCCACAGAGA CCTCCGGATG CCTCACCCAG GCATGAGGGA GCGCAAAAAC
    AGCATCACAG AGATCAGCGA CAACGAGGAT GACCTTCTGG AGTACCATCG ACGCCAAAGA
    GAAGAGCGAC TCAGAGAGCA GGAAATGGAG AGACTGGAAC GGCAGCGGCT GGAGACAATC
    TTGAACCTCT GCGCTGAGTA TAACAAGGGC GACGGGCCGC CGGGTGATTT GGGCAGGATG
    GGCGTCTCTG GGCTTGGCTC GAGGGACGGG CTTAACGTCA GGCGTCCTTC AATGGACAGC
    GTGAACAGCG CTTCACTAAG AGCGACTCAA AGACAACGGG AAAGCCAAGA GGACAACCTG
    AAGGAGGATA GCAGTAGCAC TGAGAGTGCG CAGCTGGACG GACGGACACC TGCTCTGCTA
    AATGGACAGA ATGGACAGGC TGAGGAGTCT CTGGGTTCGG GCAGCATTGG CCGTCTGGAG
    CTGGGCTACC TGGAGGAGGA GCGGGTTCGA GTGCTGGCAA AGGTAGATGA GCTCAAAGCT
    CGAATCACAG AACTGGAGCA GCAACTTCAG GAGTCTAAAC AAGAGGCGGA GATGGAGAGA
    GCTCTGCTGC AGGGAGAAAG ACAGGCTGAG ATGGAGCAAA TCGAGACTGA GACCGAGATT
    ATCAACCAGC TGCAGCGCAA ACTCACTGAA CTGGAGAGCA GCATCCAGAG AGAGAAGGAC
    AAGGCATGCC ATTCTTCCAG ATGGCCACTC AAGGCCCATT CAGGTCTCGA TTGCCATCCT
    CAGGAGAGGG CTAATGTTGA CGCGGAGCGG GAAGCCCTGG AGAGGCTTCA GGATGGGTAC
    AATGAGTTGA CGAGCCAGCT ACATAACTGC CCTGAGTCTC TGAGGGAACA GTTACAGGAA
    CAGCTCAAGA GGGAAGCTGA GACTCTTGAA GCAGCGACAA AGCAGTTTGA AGACCTGGAG
    TTTCAGCAGT TGGAGAGGGA GAGCAGTCTG GAGGAGGAGC GCGAGACCAT CAGCCAGCAG
    CTCATGCAGG ACAGAGCTCA ATACCACAGC AGTGTCGCCA AGAGAAAGGA AAAGGTGGTG
    GCATTGGAGA GCCAGGCGAG CCAGCTGGGA ATACAGGCCG CTCAGGAGTG TGAGAGACTG
    GCCAAGGACA GAACCATCAC CTTGCAGATG CTACACAAGG AAAAGGAAAG GCTGGCCAGT
    CTGGAGAGAA GATACCAGAG TTTAACTGGT GGAAAGACGT TCCCCAAAAG CTCCAATACT
    ATAAAGGAGG AGTACTTGAA GCTGTCGGAT GTGTATAAGA TGTATGGGAG TGAATGCCAT
    GACATCCAGC TCGCCAGCGC TTCCTCACAC GGCCTCTCGC TTGCCCTTGA CCCAGCTGAA
    ACTGCCCCGC GTGAGGAGTA TGTGACGGTC AGCCAATTAA ACCAGATGTA CGGGATGCCA
    AAGGTGGAGT CTTCCCCTAC CTCACCCGTC CAATCAGTGC AGCCCTCTCT CGCAGACTCC
    ACCTTCCCCC GCCTCCCTTC CCCTCATGGC TCCTCCCTCT CTCTCTCCTT CGAGCGTCAG
    TCAGACTGGG GCAGGCCTCA GATGCCCGTG CTAGATTTAG AGCACTGGTA CCAGGAGGTA
    ATGGCAGCTG GAGATGTAAA CCAGCTCTGC CCTCCTCCAT TGCCGGCTAA AGCTTTCTCA
    TCATCATCAT CATCACCACG CAGGCCTGTG CAGGTGTACA GATCTCGACT GGACAGTGAT
    TGCAGCCAAT CCTCACTTAG ACCGAAAGTC AGCGCCGGTT TGTCTCCTAC ATACACGACG
    GCCACGCTGG GACGAAACAG TCCTGCCAGG AGTCCTCTGA TGGTGGCCAA CAGCACCGGA
    AGTCTCCCAC GCAACCTGGC AGCCACCCTG CAGGACATCG AGACCAAGAG ACAGCTGGCT
    CTTCAGCAGA AAGGTCAGCA GGTTATCGAG GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG
    CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG TGGGAGGCGC TGCACGGCTC TCAAACGCAG
    CTCCACCAGC CCTTCTCATC AAGCCCAGCA CCGGCGCTTC ACCACTCCAT CCTGCACCAC
    ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC GACACGCTAA GCCTGGAGAG CTCGGACAGC
    ATGGACACCA GCATCTCTAC TGGAAACAAC TCGGCCTGCT CGCCGGACAA CATGTCCAGT
    GCCAGCGGTA TGGATGCGTT GAAGATTGAA GAGATGGAGA AGATGTTGAA GGAGGCGCAG
    CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA GAGCGAGAGA CTCAGAGCCG TAAACTGATG
    CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG GCCGAGAAGA GGCTGCAGGA GGAGACGCTG
    CACAGACAGC AGCTCATCGA GAAGGAGGTC AAGATGAGGG CCAGAAACTT CTCTCAGGCC
    CGTCCGATGA CCCGCTACCT GCCCATCCGC AAAGAGGAGT TTGACCTGCG TTCGCACATC
    GAGTCTTCAG GCCACAGCGT AGACACCTGC TACCACGTCA TCCTCACCGA GAAGATGTGC
    AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG ATCAAATCCT GGAAGAAGCG CTGGTTCGTC
    TTTGATCGCC TCAAAAGGAC GTTCTCTTAC TACGCCGACA AACACGAGAG CAAGCTGAAG
    GGTGTGATTT ACTTCCAGGC CATCGAGGAG GTTTACTACG ATCACCTGCG CAGCGCCACA
    AAGAAAGGAT TTTTCAATCT GAATCTGACA AACAGCCCGA CCCCGACGCT GACCTTCTGT
    GTTAAAACAC ACGACAGACT GTATTACATG GTGGCTCCGT CCGCAGAGGC CATGAGGATA
    TGGATGGATG TGATTGTGAC AGGAGCCGAG GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322035.1
    CDS332..5185
    Translation

    Target ORF information:

    RefSeq Version XM_021466360.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466360.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGGAGCC ATGTTGAGGG TCTTGTTTCA TTGGCTGCCA GAAAGTTATT GCAGGATTCT 
    CATGTCCCCG AGCAAGTAAG TGACAGTACA CTGGCCATGG ATACTTTGAA CCAAAATCTT
    AGCCATGGGC ATCAGTCTCA TCAGGTGCTC CAGAGTACTC CACTGGATCT TATTGAGACT
    GGAAAGGCAC TGAAGGTTCA AGCAGAGCGC CCCCATTTAG TCAGTCTGGG AAGTGGGCGT
    CTCAGTACAG CCATCACCCT CTTACCCCTG CCTGAAGGGA AGACGACTGT GGGTCATGGA
    GCCATGGACA TCAGCATACA GGGCCCCGGG GTCGCAGCTC AGCACTGCTT TATTGAGAAC
    AGGGCTGGAA TCATCACACT TCACCCCTGT GGAAACCAGT GCAGCATGGA CGGACTGCCG
    GTCAACAAAC CTGTGCGACT CTCTCAAGGC TGCATGCTGT GTTTCGGCCA ATCCGCCTTC
    TTCCGCTTCA ACCACCCCGA GGAGGCTTTC CGAATGAAAA GCATGATGCC CGAGGGAGGA
    CAGACAGCCA GTGTGAAAAA CTGGACCCAC AAAGATTCAG AGAACCTGGT CAATGGGAAC
    CACTCTTCAG GAGAGGCAAA ATCCCACTTG GTTTCCAACG AGAGAGTGCG CCCTGAGCAC
    AGTGCCATCG TCAGTTCCAT TGAGAAGGAC CTTCAGGACA TTATGGACTC ATTAGTCATG
    GATGACCCTC AGCCCTCATC CTCCGAGTCA AAGAAGCCCT CTAGTCAGTC CATCTCACAG
    TCTCCCCTTT CTCCGATGTT AAATGGAGGG GGGCGCTTCC TTCTCTCTCC CCCAACGAGC
    CCTGGTGCTA TGTCTGTGGG CTCCAGTTAT GAGAATAACT CCCCTCCTTT CTCTCCTCTG
    TCATCTCCCT CGGCTGCCAG CAGTGGCAGC TACACCACTC TCTCACCAAT TGGTTGTCAG
    GACCAGCCTC ACATGCTTCC TCCTGTGCCA GTGAGGTCTT CTAGTTACAA TTATACCAGC
    CAGCCACCCA TACCTCAGCC ACGGACCATA CTGCCCAGCT ATTCCAGTGG CAGCAGTGGG
    CCAAAGGTGC CTGAGAGTCC CAGACTCCAG CGAAAGGCGC TTTTAGAAGC ACCGCCAAGC
    CCAAAGCCAA GCCGTAGGGG GTTGAACCAA GACAGCTCGG TGGCTAAAAC CCCAGACAGC
    CCCATACAGA CTCACATTCT GCCCTCAGTC TCCATCTCTA CAGCTCCGAC TGATGTCCCA
    TCTGTTAGTC GGGTACCAGT TCCAGGTAGT CCTAGATTAG CCACCAAATT CTCCACTGCG
    TCCTCACCTG CCTCACCCTC TAGTCCCAGA ACCAAAACTA CCATTGTCCT CCAAGATCGA
    CCCTCAAGTC CTTTCCGGGA GCAACCTCAG CCAGATCGTT CTTTGACGTC CAGCCCCTCT
    CACCAACTCT CCCCACCATC AAGATCTTTC CAGCCCCCAT TGGACCCCAT TGTTCACATC
    ATTCAAGGTG GACCTTCACA CCCACATCCT CGAACACTGC AGCCTCCAGA AAGTCCGCGG
    CTGGCCCGCA GAAATCTTGA AGGGAGCAGC ATGAGGGAAC TCCCCCCTCT CAGTCCCTCC
    ATAGCTCGCA GGGGGGTTCC TGTGCTCCCA GGTGCTCTAC CAGGAACCCT GCGAACTCCA
    GAGAGCCCAT CCCCAAGAAT GGTGCCAGAG AGTCCCAGAC TCAGGAGGAA AACAGGGTCA
    CCTACAGAGG AGCCATTCAG TCCTCGTGCG ATGCGCACTC GTAGTCCTTC GCCCACCTCT
    GGACTGACTG GCGAAAGCAG TGGACGGAAG GGAAGTTTTG GGAACTCACT CTCTTCTGCC
    TTCAGCCTAA GTTCTCTGCC TGGGTCTTCC CCACGATCTA GTCCCAGGAG CCACCGAAAG
    ATGTCAGCAG GCCACAGAGA CCTCCGGATG CCTCACCCAG GCATGAGGGA GCGCAAAAAC
    AGCATCACAG AGATCAGCGA CAACGAGGAT GACCTTCTGG AGTACCATCG ACGCCAAAGA
    GAAGAGCGAC TCAGAGAGCA GGAAATGGAG AGACTGGAAC GGCAGCGGCT GGAGACAATC
    TTGAACCTCT GCGCTGAGTA TAACAAGGGC GACGGGCCGC CGGGTGATTT GGGCAGGATG
    GGCGTCTCTG GGCTTGGCTC GAGGGACGGG CTTAACGTCA GGCGTCCTTC AATGGACAGC
    GTGAACAGCG CTTCACTAAG AGCGACTCAA AGACAACGGG AAAGCCAAGA GGACAACCTG
    AAGGAGGATA GCAGTAGCAC TGAGAGTGCG CAGCTGGACG GACGGACACC TGCTCTGCTA
    AATGGACAGA ATGGACAGGC TGAGGAGTCT CTGGGTTCGG GCAGCATTGG CCGTCTGGAG
    CTGGGCTACC TGGAGGAGGA GCGGGTTCGA GTGCTGGCAA AGGTAGATGA GCTCAAAGCT
    CGAATCACAG AACTGGAGCA GCAACTTCAG GAGTCTAAAC AAGAGGCGGA GATGGAGAGA
    GCTCTGCTGC AGGGAGAAAG ACAGGCTGAG ATGGAGCAAA TCGAGACTGA GACCGAGATT
    ATCAACCAGC TGCAGCGCAA ACTCACTGAA CTGGAGAGCA GCATCCAGAG AGAGAAGGAC
    AAGGCATGCC ATTCTTCCAG ATGGCCACTC AAGGCCCATT CAGGTCTCGA TTGCCATCCT
    CAGGAGAGGG CTAATGTTGA CGCGGAGCGG GAAGCCCTGG AGAGGCTTCA GGATGGGTAC
    AATGAGTTGA CGAGCCAGCT ACATAACTGC CCTGAGTCTC TGAGGGAACA GTTACAGGAA
    CAGCTCAAGA GGGAAGCTGA GACTCTTGAA GCAGCGACAA AGCAGTTTGA AGACCTGGAG
    TTTCAGCAGT TGGAGAGGGA GAGCAGTCTG GAGGAGGAGC GCGAGACCAT CAGCCAGCAG
    CTCATGCAGG ACAGAGCTCA ATACCACAGC AGTGTCGCCA AGAGAAAGGA AAAGGTGGTG
    GCATTGGAGA GCCAGGCGAG CCAGCTGGGA ATACAGGCCG CTCAGGAGTG TGAGAGACTG
    GCCAAGGACA GAACCATCAC CTTGCAGATG CTACACAAGG AAAAGGAAAG GCTGGCCAGT
    CTGGAGAGAA GATACCAGAG TTTAACTGGT GGAAAGACGT TCCCCAAAAG CTCCAATACT
    ATAAAGGAGG AGTACTTGAA GCTGTCGGAT GTGTATAAGA TGTATGGGAG TGAATGCCAT
    GACATCCAGC TCGCCAGCGC TTCCTCACAC GGCCTCTCGC TTGCCCTTGA CCCAGCTGAA
    ACTGCCCCGC GTGAGGAGTA TGTGACGGTC AGCCAATTAA ACCAGATGTA CGGGATGCCA
    AAGGTGGAGT CTTCCCCTAC CTCACCCGTC CAATCAGTGC AGCCCTCTCT CGCAGACTCC
    ACCTTCCCCC GCCTCCCTTC CCCTCATGGC TCCTCCCTCT CTCTCTCCTT CGAGCGTCAG
    TCAGACTGGG GCAGGCCTCA GATGCCCGTG CTAGATTTAG AGCACTGGTA CCAGGAGGTA
    ATGGCAGCTG GAGATGTAAA CCAGCTCTGC CCTCCTCCAT TGCCGGCTAA AGCTTTCTCA
    TCATCATCAT CATCACCACG CAGGCCTGTG CAGGTGTACA GATCTCGACT GGACAGTGAT
    TGCAGCCAAT CCTCACTTAG ACCGAAAGTC AGCGCCGGTT TGTCTCCTAC ATACACGACG
    GCCACGCTGG GACGAAACAG TCCTGCCAGG AGTCCTCTGA TGGTGGCCAA CAGCACCGGA
    AGTCTCCCAC GCAACCTGGC AGCCACCCTG CAGGACATCG AGACCAAGAG ACAGCTGGCT
    CTTCAGCAGA AAGGTCAGCA GGTTATCGAG GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG
    CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG TGGGAGGCGC TGCACGGCTC TCAAACGCAG
    CTCCACCAGC CCTTCTCATC AAGCCCAGCA CCGGCGCTTC ACCACTCCAT CCTGCACCAC
    ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC GACACGCTAA GCCTGGAGAG CTCGGACAGC
    ATGGACACCA GCATCTCTAC TGGAAACAAC TCGGCCTGCT CGCCGGACAA CATGTCCAGT
    GCCAGCGGTA TGGATGCGTT GAAGATTGAA GAGATGGAGA AGATGTTGAA GGAGGCGCAG
    CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA GAGCGAGAGA CTCAGAGCCG TAAACTGATG
    CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG GCCGAGAAGA GGCTGCAGGA GGAGACGCTG
    CACAGACAGC AGCTCATCGA GAAGGAGGTC AAGATGAGGG CCAGAAACTT CTCTCAGGCC
    CGTCCGATGA CCCGCTACCT GCCCATCCGC AAAGAGGAGT TTGACCTGCG TTCGCACATC
    GAGTCTTCAG GCCACAGCGT AGACACCTGC TACCACGTCA TCCTCACCGA GAAGATGTGC
    AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG ATCAAATCCT GGAAGAAGCG CTGGTTCGTC
    TTTGATCGCC TCAAAAGGAC GTTCTCTTAC TACGCCGACA AACACGAGAG CAAGCTGAAG
    GGTGTGATTT ACTTCCAGGC CATCGAGGAG GTTTACTACG ATCACCTGCG CAGCGCCACA
    AAGAAAGGAT TTTTCAATCT GAATCTGACA AACAGCCCGA CCCCGACGCT GACCTTCTGT
    GTTAAAACAC ACGACAGACT GTATTACATG GTGGCTCCGT CCGCAGAGGC CATGAGGATA
    TGGATGGATG TGATTGTGAC AGGAGCCGAG GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149046
    Clone ID Related Accession (Same CDS sequence) XM_021466363.1
    Accession Version XM_021466363.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATGCTCA AGAAGGTCCG ATTTCAGGAT TCTCATGTCC CCGAGCAAGT AAGTGACAGT 
    ACACTGGCCA TGGATACTTT GAACCAAAAT CTTAGCCATG GGCATCAGTC TCATCAGGTG
    CTCCAGAGTA CTCCACTGGA TCTTATTGAG ACTGGAAAGG CACTGAAGGT TCAAGCAGAG
    CGCCCCCATT TAGTCAGTCT GGGAAGTGGG CGTCTCAGTA CAGCCATCAC CCTCTTACCC
    CTGCCTGAAG GGAAGACGAC TGTGGGTCAT GGAGCCATGG ACATCAGCAT ACAGGGCCCC
    GGGGTCGCAG CTCAGCACTG CTTTATTGAG AACAGGGCTG GAATCATCAC ACTTCACCCC
    TGTGGAAACC AGTGCAGCAT GGACGGACTG CCGGTCAACA AACCTGTGCG ACTCTCTCAA
    GGCTGCATGC TGTGTTTCGG CCAATCCGCC TTCTTCCGCT TCAACCACCC CGAGGAGGCT
    TTCCGAATGA AAAGCATGAT GCCCGAGGGA GGACAGACAG CCAGTGTGAA AAACTGGACC
    CACAAAGATT CAGAGAACCT GGTCAATGGG AACCACTCTT CAGGAGAGGC AAAATCCCAC
    TTGGTTTCCA ACGAGAGAGT GCGCCCTGAG CACAGTGCCA TCGTCAGTTC CATTGAGAAG
    GACCTTCAGG ACATTATGGA CTCATTAGTC ATGGATGACC CTCAGCCCTC ATCCTCCGAG
    TCAAAGAAGC CCTCTAGTCA GTCCATCTCA CAGTCTCCCC TTTCTCCGAT GTTAAATGGA
    GGGGGGCGCT TCCTTCTCTC TCCCCCAACG AGCCCTGGTG CTATGTCTGT GGGCTCCAGT
    TATGAGAATA ACTCCCCTCC TTTCTCTCCT CTGTCATCTC CCTCGGCTGC CAGCAGTGGC
    AGCTACACCA CTCTCTCACC AATTGGTTGT CAGGACCAGC CTCACATGCT TCCTCCTGTG
    CCAGTGAGGT CTTCTAGTTA CAATTATACC AGCCAGCCAC CCATACCTCA GCCACGGACC
    ATACTGCCCA GCTATTCCAG TGGCAGCAGT GGGCCAAAGG TGCCTGAGAG TCCCAGACTC
    CAGCGAAAGG CGCTTTTAGA AGCACCGCCA AGCCCAAAGC CAAGCCGTAG GGGGTTGAAC
    CAAGACAGCT CGGTGGCTAA AACCCCAGAC AGCCCCATAC AGACTCACAT TCTGCCCTCA
    GTCTCCATCT CTACAGCTCC GACTGATGTC CCATCTGTTA GTCGGGTACC AGTTCCAGGT
    AGTCCTAGAT TAGCCACCAA ATTCTCCACT GCGTCCTCAC CTGCCTCACC CTCTAGTCCC
    AGAACCAAAA CTACCATTGT CCTCCAAGAT CGACCCTCAA GTCCTTTCCG GGAGCAACCT
    CAGCCAGATC GTTCTTTGAC GTCCAGCCCC TCTCACCAAC TCTCCCCACC ATCAAGATCT
    TTCCAGCCCC CATTGGACCC CATTGTTCAC ATCATTCAAG GTGGACCTTC ACACCCACAT
    CCTCGAACAC TGCAGCCTCC AGAAAGTCCG CGGCTGGCCC GCAGAAATCT TGAAGGGAGC
    AGCATGAGGG AACTCCCCCC TCTCAGTCCC TCCATAGCTC GCAGGGGGGT TCCTGTGCTC
    CCAGGTGCTC TACCAGGAAC CCTGCGAACT CCAGAGAGCC CATCCCCAAG AATGGTGCCA
    GAGAGTCCCA GACTCAGGAG GAAAACAGGG TCACCTACAG AGGAGCCATT CAGTCCTCGT
    GCGATGCGCA CTCGTAGTCC TTCGCCCACC TCTGGACTGA CTGGCGAAAG CAGTGGACGG
    AAGGGAAGTT TTGGGAACTC ACTCTCTTCT GCCTTCAGCC TAAGTTCTCT GCCTGGGTCT
    TCCCCACGAT CTAGTCCCAG GAGCCACCGA AAGATGTCAG CAGGCCACAG AGACCTCCGG
    ATGCCTCACC CAGGCATGAG GGAGCGCAAA AACAGCATCA CAGAGATCAG CGACAACGAG
    GATGACCTTC TGGAGTACCA TCGACGCCAA AGAGAAGAGC GACTCAGAGA GCAGGAAATG
    GAGAGACTGG AACGGCAGCG GCTGGAGACA ATCTTGAACC TCTGCGCTGA GTATAACAAG
    GGCGACGGGC CGCCGGGTGA TTTGGGCAGG ATGGGCGTCT CTGGGCTTGG CTCGAGGGAC
    GGGCTTAACG TCAGGCGTCC TTCAATGGAC AGCGTGAACA GCGCTTCACT AAGAGCGACT
    CAAAGACAAC GGGAAAGCCA AGAGGACAAC CTGAAGGAGG ATAGCAGTAG CACTGAGAGT
    GCGCAGCTGG ACGGACGGAC ACCTGCTCTG CTAAATGGAC AGAATGGACA GGCTGAGGAG
    TCTCTGGGTT CGGGCAGCAT TGGCCGTCTG GAGCTGGGCT ACCTGGAGGA GGAGCGGGTT
    CGAGTGCTGG CAAAGGTAGA TGAGCTCAAA GCTCGAATCA CAGAACTGGA GCAGCAACTT
    CAGGAGTCTA AACAAGAGGC GGAGATGGAG AGAGCTCTGC TGCAGGGAGA AAGACAGGCT
    GAGATGGAGC AAATCGAGAC TGAGACCGAG ATTATCAACC AGCTGCAGCG CAAACTCACT
    GAACTGGAGA GCAGCATCCA GAGAGAGAAG GACAAGGCAT GCCATTCTTC CAGATGGCCA
    CTCAAGGCCC ATTCAGGTCT CGATTGCCAT CCTCAGGAGA GGGCTAATGT TGACGCGGAG
    CGGGAAGCCC TGGAGAGGCT TCAGGATGGG TACAATGAGT TGACGAGCCA GCTACATAAC
    TGCCCTGAGT CTCTGAGGGA ACAGTTACAG GAACAGCTCA AGAGGGAAGC TGAGACTCTT
    GAAGCAGCGA CAAAGCAGTT TGAAGACCTG GAGTTTCAGC AGTTGGAGAG GGAGAGCAGT
    CTGGAGGAGG AGCGCGAGAC CATCAGCCAG CAGCTCATGC AGGACAGAGC TCAATACCAC
    AGCAGTGTCG CCAAGAGAAA GGAAAAGGTG GTGGCATTGG AGAGCCAGGC GAGCCAGCTG
    GGAATACAGG CCGCTCAGGA GTGTGAGAGA CTGGCCAAGG ACAGAACCAT CACCTTGCAG
    ATGCTACACA AGGAAAAGGA AAGGCTGGCC AGTCTGGAGA GAAGATACCA GAGTTTAACT
    GGTGGAAAGA CGTTCCCCAA AAGCTCCAAT ACTATAAAGG AGGAGTACTT GAAGCTGTCG
    GATGTGTATA AGATGTATGG GAGTGAATGC CATGACATCC AGCTCGCCAG CGCTTCCTCA
    CACGGCCTCT CGCTTGCCCT TGACCCAGCT GAAACTGCCC CGCGTGAGGA GTATGTGACG
    GTCAGCCAAT TAAACCAGAT GTACGGGATG CCAAAGGTGG AGTCTTCCCC TACCTCACCC
    GTCCAATCAG TGCAGCCCTC TCTCGCAGAC TCCACCTTCC CCCGCCTCCC TTCCCCTCAT
    GGCTCCTCCC TCTCTCTCTC CTTCGAGCGT CAGTCAGACT GGGGCAGGCC TCAGATGCCC
    GTGCTAGATT TAGAGCACTG GTACCAGGAG GTAATGGCAG CTGGAGATGT AAACCAGCTC
    TGCCCTCCTC CATTGCCGGC TAAAGCTTTC TCATCATCAT CATCATCACC ACGCAGGCCT
    GTGCAGGTGT ACAGATCTCG ACTGGACAGT GATTGCAGCC AATCCTCACT TAGACCGAAA
    GTCAGCGCCG GTTTGTCTCC TACATACACG ACGGCCACGC TGGGACGAAA CAGTCCTGCC
    AGGAGTCCTC TGATGGTGGC CAACAGCACC GGAAGTCTCC CACGCAACCT GGCAGCCACC
    CTGCAGGACA TCGAGACCAA GAGACAGCTG GCTCTTCAGC AGAAAGGTCA GCAGGTTATC
    GAGGAGCAGC GCAGGCGTCT GGCGGAGCTG AAGCAGAAAG CTGCAGTGGA GGCTCAGTGC
    CAGTGGGAGG CGCTGCACGG CTCTCAAACG CAGCTCCACC AGCCCTTCTC ATCAAGCCCA
    GCACCGGCGC TTCACCACTC CATCCTGCAC CACACAGCGC CCCCTTGCGG AGAGCAGCCG
    TACGACACGC TAAGCCTGGA GAGCTCGGAC AGCATGGACA CCAGCATCTC TACTGGAAAC
    AACTCGGCCT GCTCGCCGGA CAACATGTCC AGTGCCAGCG GTATGGATGC GTTGAAGATT
    GAAGAGATGG AGAAGATGTT GAAGGAGGCG CAGCTGGAAA AAGCTCGGCT CATAGAGTCT
    AGAGAGCGAG AGACTCAGAG CCGTAAACTG ATGCTGGAGG AGGAGCGGAG GAGGAGAGAG
    GAGGCCGAGA AGAGGCTGCA GGAGGAGACG CTGCACAGAC AGCAGCTCAT CGAGAAGGAG
    GTCAAGATGA GGGCCAGAAA CTTCTCTCAG GCCCGTCCGA TGACCCGCTA CCTGCCCATC
    CGCAAAGAGG AGTTTGACCT GCGTTCGCAC ATCGAGTCTT CAGGCCACAG CGTAGACACC
    TGCTACCACG TCATCCTCAC CGAGAAGATG TGCAAGGGCT TTCTGGTCAA GATGGGAGGC
    AAGATCAAAT CCTGGAAGAA GCGCTGGTTC GTCTTTGATC GCCTCAAAAG GACGTTCTCT
    TACTACGCCG ACAAACACGA GAGCAAGCTG AAGGGTGTGA TTTACTTCCA GGCCATCGAG
    GAGGTTTACT ACGATCACCT GCGCAGCGCC ACAAAGAAAG GATTTTTCAA TCTGAATCTG
    ACAAACAGCC CGACCCCGAC GCTGACCTTC TGTGTTAAAA CACACGACAG ACTGTATTAC
    ATGGTGGCTC CGTCCGCAGA GGCCATGAGG ATATGGATGG ATGTGATTGT GACAGGAGCC
    GAGGGCTACA CACAGTTCAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322038.1
    CDS271..5097
    Translation

    Target ORF information:

    RefSeq Version XM_021466363.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466363.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGATGCTCA AGAAGGTCCG ATTTCAGGAT TCTCATGTCC CCGAGCAAGT AAGTGACAGT 
    ACACTGGCCA TGGATACTTT GAACCAAAAT CTTAGCCATG GGCATCAGTC TCATCAGGTG
    CTCCAGAGTA CTCCACTGGA TCTTATTGAG ACTGGAAAGG CACTGAAGGT TCAAGCAGAG
    CGCCCCCATT TAGTCAGTCT GGGAAGTGGG CGTCTCAGTA CAGCCATCAC CCTCTTACCC
    CTGCCTGAAG GGAAGACGAC TGTGGGTCAT GGAGCCATGG ACATCAGCAT ACAGGGCCCC
    GGGGTCGCAG CTCAGCACTG CTTTATTGAG AACAGGGCTG GAATCATCAC ACTTCACCCC
    TGTGGAAACC AGTGCAGCAT GGACGGACTG CCGGTCAACA AACCTGTGCG ACTCTCTCAA
    GGCTGCATGC TGTGTTTCGG CCAATCCGCC TTCTTCCGCT TCAACCACCC CGAGGAGGCT
    TTCCGAATGA AAAGCATGAT GCCCGAGGGA GGACAGACAG CCAGTGTGAA AAACTGGACC
    CACAAAGATT CAGAGAACCT GGTCAATGGG AACCACTCTT CAGGAGAGGC AAAATCCCAC
    TTGGTTTCCA ACGAGAGAGT GCGCCCTGAG CACAGTGCCA TCGTCAGTTC CATTGAGAAG
    GACCTTCAGG ACATTATGGA CTCATTAGTC ATGGATGACC CTCAGCCCTC ATCCTCCGAG
    TCAAAGAAGC CCTCTAGTCA GTCCATCTCA CAGTCTCCCC TTTCTCCGAT GTTAAATGGA
    GGGGGGCGCT TCCTTCTCTC TCCCCCAACG AGCCCTGGTG CTATGTCTGT GGGCTCCAGT
    TATGAGAATA ACTCCCCTCC TTTCTCTCCT CTGTCATCTC CCTCGGCTGC CAGCAGTGGC
    AGCTACACCA CTCTCTCACC AATTGGTTGT CAGGACCAGC CTCACATGCT TCCTCCTGTG
    CCAGTGAGGT CTTCTAGTTA CAATTATACC AGCCAGCCAC CCATACCTCA GCCACGGACC
    ATACTGCCCA GCTATTCCAG TGGCAGCAGT GGGCCAAAGG TGCCTGAGAG TCCCAGACTC
    CAGCGAAAGG CGCTTTTAGA AGCACCGCCA AGCCCAAAGC CAAGCCGTAG GGGGTTGAAC
    CAAGACAGCT CGGTGGCTAA AACCCCAGAC AGCCCCATAC AGACTCACAT TCTGCCCTCA
    GTCTCCATCT CTACAGCTCC GACTGATGTC CCATCTGTTA GTCGGGTACC AGTTCCAGGT
    AGTCCTAGAT TAGCCACCAA ATTCTCCACT GCGTCCTCAC CTGCCTCACC CTCTAGTCCC
    AGAACCAAAA CTACCATTGT CCTCCAAGAT CGACCCTCAA GTCCTTTCCG GGAGCAACCT
    CAGCCAGATC GTTCTTTGAC GTCCAGCCCC TCTCACCAAC TCTCCCCACC ATCAAGATCT
    TTCCAGCCCC CATTGGACCC CATTGTTCAC ATCATTCAAG GTGGACCTTC ACACCCACAT
    CCTCGAACAC TGCAGCCTCC AGAAAGTCCG CGGCTGGCCC GCAGAAATCT TGAAGGGAGC
    AGCATGAGGG AACTCCCCCC TCTCAGTCCC TCCATAGCTC GCAGGGGGGT TCCTGTGCTC
    CCAGGTGCTC TACCAGGAAC CCTGCGAACT CCAGAGAGCC CATCCCCAAG AATGGTGCCA
    GAGAGTCCCA GACTCAGGAG GAAAACAGGG TCACCTACAG AGGAGCCATT CAGTCCTCGT
    GCGATGCGCA CTCGTAGTCC TTCGCCCACC TCTGGACTGA CTGGCGAAAG CAGTGGACGG
    AAGGGAAGTT TTGGGAACTC ACTCTCTTCT GCCTTCAGCC TAAGTTCTCT GCCTGGGTCT
    TCCCCACGAT CTAGTCCCAG GAGCCACCGA AAGATGTCAG CAGGCCACAG AGACCTCCGG
    ATGCCTCACC CAGGCATGAG GGAGCGCAAA AACAGCATCA CAGAGATCAG CGACAACGAG
    GATGACCTTC TGGAGTACCA TCGACGCCAA AGAGAAGAGC GACTCAGAGA GCAGGAAATG
    GAGAGACTGG AACGGCAGCG GCTGGAGACA ATCTTGAACC TCTGCGCTGA GTATAACAAG
    GGCGACGGGC CGCCGGGTGA TTTGGGCAGG ATGGGCGTCT CTGGGCTTGG CTCGAGGGAC
    GGGCTTAACG TCAGGCGTCC TTCAATGGAC AGCGTGAACA GCGCTTCACT AAGAGCGACT
    CAAAGACAAC GGGAAAGCCA AGAGGACAAC CTGAAGGAGG ATAGCAGTAG CACTGAGAGT
    GCGCAGCTGG ACGGACGGAC ACCTGCTCTG CTAAATGGAC AGAATGGACA GGCTGAGGAG
    TCTCTGGGTT CGGGCAGCAT TGGCCGTCTG GAGCTGGGCT ACCTGGAGGA GGAGCGGGTT
    CGAGTGCTGG CAAAGGTAGA TGAGCTCAAA GCTCGAATCA CAGAACTGGA GCAGCAACTT
    CAGGAGTCTA AACAAGAGGC GGAGATGGAG AGAGCTCTGC TGCAGGGAGA AAGACAGGCT
    GAGATGGAGC AAATCGAGAC TGAGACCGAG ATTATCAACC AGCTGCAGCG CAAACTCACT
    GAACTGGAGA GCAGCATCCA GAGAGAGAAG GACAAGGCAT GCCATTCTTC CAGATGGCCA
    CTCAAGGCCC ATTCAGGTCT CGATTGCCAT CCTCAGGAGA GGGCTAATGT TGACGCGGAG
    CGGGAAGCCC TGGAGAGGCT TCAGGATGGG TACAATGAGT TGACGAGCCA GCTACATAAC
    TGCCCTGAGT CTCTGAGGGA ACAGTTACAG GAACAGCTCA AGAGGGAAGC TGAGACTCTT
    GAAGCAGCGA CAAAGCAGTT TGAAGACCTG GAGTTTCAGC AGTTGGAGAG GGAGAGCAGT
    CTGGAGGAGG AGCGCGAGAC CATCAGCCAG CAGCTCATGC AGGACAGAGC TCAATACCAC
    AGCAGTGTCG CCAAGAGAAA GGAAAAGGTG GTGGCATTGG AGAGCCAGGC GAGCCAGCTG
    GGAATACAGG CCGCTCAGGA GTGTGAGAGA CTGGCCAAGG ACAGAACCAT CACCTTGCAG
    ATGCTACACA AGGAAAAGGA AAGGCTGGCC AGTCTGGAGA GAAGATACCA GAGTTTAACT
    GGTGGAAAGA CGTTCCCCAA AAGCTCCAAT ACTATAAAGG AGGAGTACTT GAAGCTGTCG
    GATGTGTATA AGATGTATGG GAGTGAATGC CATGACATCC AGCTCGCCAG CGCTTCCTCA
    CACGGCCTCT CGCTTGCCCT TGACCCAGCT GAAACTGCCC CGCGTGAGGA GTATGTGACG
    GTCAGCCAAT TAAACCAGAT GTACGGGATG CCAAAGGTGG AGTCTTCCCC TACCTCACCC
    GTCCAATCAG TGCAGCCCTC TCTCGCAGAC TCCACCTTCC CCCGCCTCCC TTCCCCTCAT
    GGCTCCTCCC TCTCTCTCTC CTTCGAGCGT CAGTCAGACT GGGGCAGGCC TCAGATGCCC
    GTGCTAGATT TAGAGCACTG GTACCAGGAG GTAATGGCAG CTGGAGATGT AAACCAGCTC
    TGCCCTCCTC CATTGCCGGC TAAAGCTTTC TCATCATCAT CATCATCACC ACGCAGGCCT
    GTGCAGGTGT ACAGATCTCG ACTGGACAGT GATTGCAGCC AATCCTCACT TAGACCGAAA
    GTCAGCGCCG GTTTGTCTCC TACATACACG ACGGCCACGC TGGGACGAAA CAGTCCTGCC
    AGGAGTCCTC TGATGGTGGC CAACAGCACC GGAAGTCTCC CACGCAACCT GGCAGCCACC
    CTGCAGGACA TCGAGACCAA GAGACAGCTG GCTCTTCAGC AGAAAGGTCA GCAGGTTATC
    GAGGAGCAGC GCAGGCGTCT GGCGGAGCTG AAGCAGAAAG CTGCAGTGGA GGCTCAGTGC
    CAGTGGGAGG CGCTGCACGG CTCTCAAACG CAGCTCCACC AGCCCTTCTC ATCAAGCCCA
    GCACCGGCGC TTCACCACTC CATCCTGCAC CACACAGCGC CCCCTTGCGG AGAGCAGCCG
    TACGACACGC TAAGCCTGGA GAGCTCGGAC AGCATGGACA CCAGCATCTC TACTGGAAAC
    AACTCGGCCT GCTCGCCGGA CAACATGTCC AGTGCCAGCG GTATGGATGC GTTGAAGATT
    GAAGAGATGG AGAAGATGTT GAAGGAGGCG CAGCTGGAAA AAGCTCGGCT CATAGAGTCT
    AGAGAGCGAG AGACTCAGAG CCGTAAACTG ATGCTGGAGG AGGAGCGGAG GAGGAGAGAG
    GAGGCCGAGA AGAGGCTGCA GGAGGAGACG CTGCACAGAC AGCAGCTCAT CGAGAAGGAG
    GTCAAGATGA GGGCCAGAAA CTTCTCTCAG GCCCGTCCGA TGACCCGCTA CCTGCCCATC
    CGCAAAGAGG AGTTTGACCT GCGTTCGCAC ATCGAGTCTT CAGGCCACAG CGTAGACACC
    TGCTACCACG TCATCCTCAC CGAGAAGATG TGCAAGGGCT TTCTGGTCAA GATGGGAGGC
    AAGATCAAAT CCTGGAAGAA GCGCTGGTTC GTCTTTGATC GCCTCAAAAG GACGTTCTCT
    TACTACGCCG ACAAACACGA GAGCAAGCTG AAGGGTGTGA TTTACTTCCA GGCCATCGAG
    GAGGTTTACT ACGATCACCT GCGCAGCGCC ACAAAGAAAG GATTTTTCAA TCTGAATCTG
    ACAAACAGCC CGACCCCGAC GCTGACCTTC TGTGTTAAAA CACACGACAG ACTGTATTAC
    ATGGTGGCTC CGTCCGCAGA GGCCATGAGG ATATGGATGG ATGTGATTGT GACAGGAGCC
    GAGGGCTACA CACAGTTCAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149047
    Clone ID Related Accession (Same CDS sequence) XM_021466365.1
    Accession Version XM_021466365.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGCATGCCA TTCTTCCAGA TGGCCACTCA AGGCCCATTC AGGTCTCGAT
    TGCCATCCTC AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGGTGTACA GATCTCGACT GGACAGTGAT TGCAGCCAAT CCTCACTTAG ACCGAAAGTC
    AGCGCCGGTT TGTCTCCTAC ATACACGACG GCCACGCTGG GACGAAACAG TCCTGCCAGG
    AGTCCTCTGA TGGTGGCCAA CAGCACCGGA AGTCTCCCAC GCAACCTGGC AGCCACCCTG
    CAGGACATCG AGACCAAGAG ACAGCTGGCT CTTCAGCAGA AAGGTCAGCA GGTTATCGAG
    GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG
    TGGGAGGCGC TGCACGGCTC TCAAACGCAG CTCCACCAGC CCTTCTCATC AAGCCCAGCA
    CCGGCGCTTC ACCACTCCAT CCTGCACCAC ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC
    GACACGCTAA GCCTGGAGAG CTCGGACAGC ATGGACACCA GCATCTCTAC TGGAAACAAC
    TCGGCCTGCT CGCCGGACAA CATGTCCAGT GCCAGCGGTA TGGATGCGTT GAAGATTGAA
    GAGATGGAGA AGATGTTGAA GGAGGCGCAG CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA
    GAGCGAGAGA CTCAGAGCCG TAAACTGATG CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG
    GCCGAGAAGA GGCTGCAGGA GGAGACGCTG CACAGACAGC AGCTCATCGA GAAGGAGGTC
    AAGATGAGGG CCAGAAACTT CTCTCAGGCC CGTCCGATGA CCCGCTACCT GCCCATCCGC
    AAAGAGGAGT TTGACCTGCG TTCGCACATC GAGTCTTCAG GCCACAGCGT AGACACCTGC
    TACCACGTCA TCCTCACCGA GAAGATGTGC AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG
    ATCAAATCCT GGAAGAAGCG CTGGTTCGTC TTTGATCGCC TCAAAAGGAC GTTCTCTTAC
    TACGCCGACA AACACGAGAG CAAGCTGAAG GGTGTGATTT ACTTCCAGGC CATCGAGGAG
    GTTTACTACG ATCACCTGCG CAGCGCCACA AAGAAAGGAT TTTTCAATCT GAATCTGACA
    AACAGCCCGA CCCCGACGCT GACCTTCTGT GTTAAAACAC ACGACAGACT GTATTACATG
    GTGGCTCCGT CCGCAGAGGC CATGAGGATA TGGATGGATG TGATTGTGAC AGGAGCCGAG
    GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322040.1
    CDS473..5176
    Translation

    Target ORF information:

    RefSeq Version XM_021466365.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466365.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGCATGCCA TTCTTCCAGA TGGCCACTCA AGGCCCATTC AGGTCTCGAT
    TGCCATCCTC AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGGTGTACA GATCTCGACT GGACAGTGAT TGCAGCCAAT CCTCACTTAG ACCGAAAGTC
    AGCGCCGGTT TGTCTCCTAC ATACACGACG GCCACGCTGG GACGAAACAG TCCTGCCAGG
    AGTCCTCTGA TGGTGGCCAA CAGCACCGGA AGTCTCCCAC GCAACCTGGC AGCCACCCTG
    CAGGACATCG AGACCAAGAG ACAGCTGGCT CTTCAGCAGA AAGGTCAGCA GGTTATCGAG
    GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG
    TGGGAGGCGC TGCACGGCTC TCAAACGCAG CTCCACCAGC CCTTCTCATC AAGCCCAGCA
    CCGGCGCTTC ACCACTCCAT CCTGCACCAC ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC
    GACACGCTAA GCCTGGAGAG CTCGGACAGC ATGGACACCA GCATCTCTAC TGGAAACAAC
    TCGGCCTGCT CGCCGGACAA CATGTCCAGT GCCAGCGGTA TGGATGCGTT GAAGATTGAA
    GAGATGGAGA AGATGTTGAA GGAGGCGCAG CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA
    GAGCGAGAGA CTCAGAGCCG TAAACTGATG CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG
    GCCGAGAAGA GGCTGCAGGA GGAGACGCTG CACAGACAGC AGCTCATCGA GAAGGAGGTC
    AAGATGAGGG CCAGAAACTT CTCTCAGGCC CGTCCGATGA CCCGCTACCT GCCCATCCGC
    AAAGAGGAGT TTGACCTGCG TTCGCACATC GAGTCTTCAG GCCACAGCGT AGACACCTGC
    TACCACGTCA TCCTCACCGA GAAGATGTGC AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG
    ATCAAATCCT GGAAGAAGCG CTGGTTCGTC TTTGATCGCC TCAAAAGGAC GTTCTCTTAC
    TACGCCGACA AACACGAGAG CAAGCTGAAG GGTGTGATTT ACTTCCAGGC CATCGAGGAG
    GTTTACTACG ATCACCTGCG CAGCGCCACA AAGAAAGGAT TTTTCAATCT GAATCTGACA
    AACAGCCCGA CCCCGACGCT GACCTTCTGT GTTAAAACAC ACGACAGACT GTATTACATG
    GTGGCTCCGT CCGCAGAGGC CATGAGGATA TGGATGGATG TGATTGTGAC AGGAGCCGAG
    GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149048
    Clone ID Related Accession (Same CDS sequence) XM_021466366.1
    Accession Version XM_021466366.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAAGCTGA GACTCTTGAA GCAGCGACAA AGCAGTTTGA AGACCTGGAG
    TTTCAGCAGT TGGAGAGGGA GAGCAGTCTG GAGGAGGAGC GCGAGACCAT CAGCCAGCAG
    CTCATGCAGG ACAGAGCTCA ATACCACAGC AGTGTCGCCA AGAGAAAGGA AAAGGTGGTG
    GCATTGGAGA GCCAGGCGAG CCAGCTGGGA ATACAGGCCG CTCAGGAGTG TGAGAGACTG
    GCCAAGGACA GAACCATCAC CTTGCAGATG CTACACAAGG AAAAGGAAAG GCTGGCCAGT
    CTGGAGAGAA GATACCAGAG TTTAACTGGT GGAAAGACGT TCCCCAAAAG CTCCAATACT
    ATAAAGGAGG AGTACTTGAA GCTGTCGGAT GTGTATAAGA TGTATGGGAG TGAATGCCAT
    GACATCCAGC TCGCCAGCGC TTCCTCACAC GGCCTCTCGC TTGCCCTTGA CCCAGCTGAA
    ACTGCCCCGC GTGAGGAGTA TGTGACGGTC AGCCAATTAA ACCAGATGTA CGGGATGCCA
    AAGGTGGAGT CTTCCCCTAC CTCACCCGTC CAATCAGTGC AGCCCTCTCT CGCAGACTCC
    ACCTTCCCCC GCCTCCCTTC CCCTCATGGC TCCTCCCTCT CTCTCTCCTT CGAGCGTCAG
    TCAGACTGGG GCAGGCCTCA GATGCCCGTG CTAGATTTAG AGCACTGGTA CCAGGAGGTA
    ATGGCAGCTG GAGATGTAAA CCAGCTCTGC CCTCCTCCAT TGCCGGCTAA AGCTTTCTCA
    TCATCATCAT CATCACCACG CAGGCCTGTG CAGGTGTACA GATCTCGACT GGACAGTGAT
    TGCAGCCAAT CCTCACTTAG ACCGAAAGTC AGCGCCGGTT TGTCTCCTAC ATACACGACG
    GCCACGCTGG GACGAAACAG TCCTGCCAGG AGTCCTCTGA TGGTGGCCAA CAGCACCGGA
    AGTCTCCCAC GCAACCTGGC AGCCACCCTG CAGGACATCG AGACCAAGAG ACAGCTGGCT
    CTTCAGCAGA AAGGTCAGCA GGTTATCGAG GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG
    CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG TGGGAGGCGC TGCACGGCTC TCAAACGCAG
    CTCCACCAGC CCTTCTCATC AAGCCCAGCA CCGGCGCTTC ACCACTCCAT CCTGCACCAC
    ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC GACACGCTAA GCCTGGAGAG CTCGGACAGC
    ATGGACACCA GCATCTCTAC TGGAAACAAC TCGGCCTGCT CGCCGGACAA CATGTCCAGT
    GCCAGCGGTA TGGATGCGTT GAAGATTGAA GAGATGGAGA AGATGTTGAA GGAGGCGCAG
    CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA GAGCGAGAGA CTCAGAGCCG TAAACTGATG
    CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG GCCGAGAAGA GGCTGCAGGA GGAGACGCTG
    CACAGACAGC AGCTCATCGA GAAGGAGGTC AAGATGAGGG CCAGAAACTT CTCTCAGGCC
    CGTCCGATGA CCCGCTACCT GCCCATCCGC AAAGAGGAGT TTGACCTGCG TTCGCACATC
    GAGTCTTCAG GCCACAGCGT AGACACCTGC TACCACGTCA TCCTCACCGA GAAGATGTGC
    AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG ATCAAATCCT GGAAGAAGCG CTGGTTCGTC
    TTTGATCGCC TCAAAAGGAC GTTCTCTTAC TACGCCGACA AACACGAGAG CAAGCTGAAG
    GGTGTGATTT ACTTCCAGGC CATCGAGGAG GTTTACTACG ATCACCTGCG CAGCGCCACA
    AAGAAAGGAT TTTTCAATCT GAATCTGACA AACAGCCCGA CCCCGACGCT GACCTTCTGT
    GTTAAAACAC ACGACAGACT GTATTACATG GTGGCTCCGT CCGCAGAGGC CATGAGGATA
    TGGATGGATG TGATTGTGAC AGGAGCCGAG GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322041.1
    CDS474..5147
    Translation

    Target ORF information:

    RefSeq Version XM_021466366.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466366.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAAGCTGA GACTCTTGAA GCAGCGACAA AGCAGTTTGA AGACCTGGAG
    TTTCAGCAGT TGGAGAGGGA GAGCAGTCTG GAGGAGGAGC GCGAGACCAT CAGCCAGCAG
    CTCATGCAGG ACAGAGCTCA ATACCACAGC AGTGTCGCCA AGAGAAAGGA AAAGGTGGTG
    GCATTGGAGA GCCAGGCGAG CCAGCTGGGA ATACAGGCCG CTCAGGAGTG TGAGAGACTG
    GCCAAGGACA GAACCATCAC CTTGCAGATG CTACACAAGG AAAAGGAAAG GCTGGCCAGT
    CTGGAGAGAA GATACCAGAG TTTAACTGGT GGAAAGACGT TCCCCAAAAG CTCCAATACT
    ATAAAGGAGG AGTACTTGAA GCTGTCGGAT GTGTATAAGA TGTATGGGAG TGAATGCCAT
    GACATCCAGC TCGCCAGCGC TTCCTCACAC GGCCTCTCGC TTGCCCTTGA CCCAGCTGAA
    ACTGCCCCGC GTGAGGAGTA TGTGACGGTC AGCCAATTAA ACCAGATGTA CGGGATGCCA
    AAGGTGGAGT CTTCCCCTAC CTCACCCGTC CAATCAGTGC AGCCCTCTCT CGCAGACTCC
    ACCTTCCCCC GCCTCCCTTC CCCTCATGGC TCCTCCCTCT CTCTCTCCTT CGAGCGTCAG
    TCAGACTGGG GCAGGCCTCA GATGCCCGTG CTAGATTTAG AGCACTGGTA CCAGGAGGTA
    ATGGCAGCTG GAGATGTAAA CCAGCTCTGC CCTCCTCCAT TGCCGGCTAA AGCTTTCTCA
    TCATCATCAT CATCACCACG CAGGCCTGTG CAGGTGTACA GATCTCGACT GGACAGTGAT
    TGCAGCCAAT CCTCACTTAG ACCGAAAGTC AGCGCCGGTT TGTCTCCTAC ATACACGACG
    GCCACGCTGG GACGAAACAG TCCTGCCAGG AGTCCTCTGA TGGTGGCCAA CAGCACCGGA
    AGTCTCCCAC GCAACCTGGC AGCCACCCTG CAGGACATCG AGACCAAGAG ACAGCTGGCT
    CTTCAGCAGA AAGGTCAGCA GGTTATCGAG GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG
    CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG TGGGAGGCGC TGCACGGCTC TCAAACGCAG
    CTCCACCAGC CCTTCTCATC AAGCCCAGCA CCGGCGCTTC ACCACTCCAT CCTGCACCAC
    ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC GACACGCTAA GCCTGGAGAG CTCGGACAGC
    ATGGACACCA GCATCTCTAC TGGAAACAAC TCGGCCTGCT CGCCGGACAA CATGTCCAGT
    GCCAGCGGTA TGGATGCGTT GAAGATTGAA GAGATGGAGA AGATGTTGAA GGAGGCGCAG
    CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA GAGCGAGAGA CTCAGAGCCG TAAACTGATG
    CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG GCCGAGAAGA GGCTGCAGGA GGAGACGCTG
    CACAGACAGC AGCTCATCGA GAAGGAGGTC AAGATGAGGG CCAGAAACTT CTCTCAGGCC
    CGTCCGATGA CCCGCTACCT GCCCATCCGC AAAGAGGAGT TTGACCTGCG TTCGCACATC
    GAGTCTTCAG GCCACAGCGT AGACACCTGC TACCACGTCA TCCTCACCGA GAAGATGTGC
    AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG ATCAAATCCT GGAAGAAGCG CTGGTTCGTC
    TTTGATCGCC TCAAAAGGAC GTTCTCTTAC TACGCCGACA AACACGAGAG CAAGCTGAAG
    GGTGTGATTT ACTTCCAGGC CATCGAGGAG GTTTACTACG ATCACCTGCG CAGCGCCACA
    AAGAAAGGAT TTTTCAATCT GAATCTGACA AACAGCCCGA CCCCGACGCT GACCTTCTGT
    GTTAAAACAC ACGACAGACT GTATTACATG GTGGCTCCGT CCGCAGAGGC CATGAGGATA
    TGGATGGATG TGATTGTGAC AGGAGCCGAG GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149049
    Clone ID Related Accession (Same CDS sequence) XM_021466367.1
    Accession Version XM_021466367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGCATGCCA TTCTTCCAGA TGGCCACTCA AGGCCCATTC AGGTCTCGAT
    TGCCATCCTC AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGTGTAC AGATCTCGAC TGGACAGTGA TTGCAGCCAA
    TCCTCACTTA GACCGAAAGT CAGCGCCGGT TTGTCTCCTA CATACACGAC GGCCACGCTG
    GGACGAAACA GTCCTGCCAG GAGTCCTCTG ATGGTGGCCA ACAGCACCGG AAGTCTCCCA
    CGCAACCTGG CAGCCACCCT GCAGGACATC GAGACCAAGA GACAGCTGGC TCTTCAGCAG
    AAAGGTCAGC AGGTTATCGA GGAGCAGCGC AGGCGTCTGG CGGAGCTGAA GCAGAAAGCT
    GCAGTGGAGG CTCAGTGCCA GTGGGAGGCG CTGCACGGCT CTCAAACGCA GCTCCACCAG
    CCCTTCTCAT CAAGCCCAGC ACCGGCGCTT CACCACTCCA TCCTGCACCA CACAGCGCCC
    CCTTGCGGAG AGCAGCCGTA CGACACGCTA AGCCTGGAGA GCTCGGACAG CATGGACACC
    AGCATCTCTA CTGGAAACAA CTCGGCCTGC TCGCCGGACA ACATGTCCAG TGCCAGCGGT
    ATGGATGCGT TGAAGATTGA AGAGATGGAG AAGATGTTGA AGGAGGCGCA GCTGGAAAAA
    GCTCGGCTCA TAGAGTCTAG AGAGCGAGAG ACTCAGAGCC GTAAACTGAT GCTGGAGGAG
    GAGCGGAGGA GGAGAGAGGA GGCCGAGAAG AGGCTGCAGG AGGAGACGCT GCACAGACAG
    CAGCTCATCG AGAAGGAGGT CAAGATGAGG GCCAGAAACT TCTCTCAGGC CCGTCCGATG
    ACCCGCTACC TGCCCATCCG CAAAGAGGAG TTTGACCTGC GTTCGCACAT CGAGTCTTCA
    GGCCACAGCG TAGACACCTG CTACCACGTC ATCCTCACCG AGAAGATGTG CAAGGGCTTT
    CTGGTCAAGA TGGGAGGCAA GATCAAATCC TGGAAGAAGC GCTGGTTCGT CTTTGATCGC
    CTCAAAAGGA CGTTCTCTTA CTACGCCGAC AAACACGAGA GCAAGCTGAA GGGTGTGATT
    TACTTCCAGG CCATCGAGGA GGTTTACTAC GATCACCTGC GCAGCGCCAC AAAGAAAGGA
    TTTTTCAATC TGAATCTGAC AAACAGCCCG ACCCCGACGC TGACCTTCTG TGTTAAAACA
    CACGACAGAC TGTATTACAT GGTGGCTCCG TCCGCAGAGG CCATGAGGAT ATGGATGGAT
    GTGATTGTGA CAGGAGCCGA GGGCTACACA CAGTTCATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322042.1
    CDS473..5017
    Translation

    Target ORF information:

    RefSeq Version XM_021466367.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGCATGCCA TTCTTCCAGA TGGCCACTCA AGGCCCATTC AGGTCTCGAT
    TGCCATCCTC AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGTGTAC AGATCTCGAC TGGACAGTGA TTGCAGCCAA
    TCCTCACTTA GACCGAAAGT CAGCGCCGGT TTGTCTCCTA CATACACGAC GGCCACGCTG
    GGACGAAACA GTCCTGCCAG GAGTCCTCTG ATGGTGGCCA ACAGCACCGG AAGTCTCCCA
    CGCAACCTGG CAGCCACCCT GCAGGACATC GAGACCAAGA GACAGCTGGC TCTTCAGCAG
    AAAGGTCAGC AGGTTATCGA GGAGCAGCGC AGGCGTCTGG CGGAGCTGAA GCAGAAAGCT
    GCAGTGGAGG CTCAGTGCCA GTGGGAGGCG CTGCACGGCT CTCAAACGCA GCTCCACCAG
    CCCTTCTCAT CAAGCCCAGC ACCGGCGCTT CACCACTCCA TCCTGCACCA CACAGCGCCC
    CCTTGCGGAG AGCAGCCGTA CGACACGCTA AGCCTGGAGA GCTCGGACAG CATGGACACC
    AGCATCTCTA CTGGAAACAA CTCGGCCTGC TCGCCGGACA ACATGTCCAG TGCCAGCGGT
    ATGGATGCGT TGAAGATTGA AGAGATGGAG AAGATGTTGA AGGAGGCGCA GCTGGAAAAA
    GCTCGGCTCA TAGAGTCTAG AGAGCGAGAG ACTCAGAGCC GTAAACTGAT GCTGGAGGAG
    GAGCGGAGGA GGAGAGAGGA GGCCGAGAAG AGGCTGCAGG AGGAGACGCT GCACAGACAG
    CAGCTCATCG AGAAGGAGGT CAAGATGAGG GCCAGAAACT TCTCTCAGGC CCGTCCGATG
    ACCCGCTACC TGCCCATCCG CAAAGAGGAG TTTGACCTGC GTTCGCACAT CGAGTCTTCA
    GGCCACAGCG TAGACACCTG CTACCACGTC ATCCTCACCG AGAAGATGTG CAAGGGCTTT
    CTGGTCAAGA TGGGAGGCAA GATCAAATCC TGGAAGAAGC GCTGGTTCGT CTTTGATCGC
    CTCAAAAGGA CGTTCTCTTA CTACGCCGAC AAACACGAGA GCAAGCTGAA GGGTGTGATT
    TACTTCCAGG CCATCGAGGA GGTTTACTAC GATCACCTGC GCAGCGCCAC AAAGAAAGGA
    TTTTTCAATC TGAATCTGAC AAACAGCCCG ACCCCGACGC TGACCTTCTG TGTTAAAACA
    CACGACAGAC TGTATTACAT GGTGGCTCCG TCCGCAGAGG CCATGAGGAT ATGGATGGAT
    GTGATTGTGA CAGGAGCCGA GGGCTACACA CAGTTCATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149050
    Clone ID Related Accession (Same CDS sequence) XM_021466372.1
    Accession Version XM_021466372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAAGCTGA GACTCTTGAA GCAGCGACAA AGCAGTTTGA AGACCTGGAG
    TTTCAGCAGT TGGAGAGGGA GAGCAGTCTG GAGGAGGAGC GCGAGACCAT CAGCCAGCAG
    CTCATGCAGG ACAGAGCTCA ATACCACAGC AGTGTCGCCA AGAGAAAGGA AAAGGTGGTG
    GCATTGGAGA GCCAGGCGAG CCAGCTGGGA ATACAGGCCG CTCAGGAGTG TGAGAGACTG
    GCCAAGGACA GAACCATCAC CTTGCAGATG CTACACAAGG AAAAGGAAAG GCTGGCCAGT
    CTGGAGAGAA GATACCAGAG TTTAACTGGT GGAAAGACGT TCCCCAAAAG CTCCAATACT
    ATAAAGGAGG AGTACTTGAA GCTGTCGGAT GTGTATAAGA TGTATGGGAG TGAATGCCAT
    GACATCCAGC TCGCCAGCGC TTCCTCACAC GGCCTCTCGC TTGCCCTTGA CCCAGCTGAA
    ACTGCCCCGC GTGAGGAGTA TGTGACGGTC AGCCAATTAA ACCAGATGTA CGGGATGCCA
    AAGGTGGAGT CTTCCCCTAC CTCACCCGTC CAATCAGTGC AGCCCTCTCT CGCAGACTCC
    ACCTTCCCCC GCCTCCCTTC CCCTCATGGC TCCTCCCTCT CTCTCTCCTT CGAGCGTCAG
    TCAGACTGGG GCAGGCCTCA GATGCCCGTG CTAGATTTAG AGCACTGGTA CCAGGAGGTA
    ATGGCAGCTG GAGATGTAAA CCAGCTCTGC CCTCCTCCAT TGCCGGCTAA AGCTTTCTCA
    TCATCATCAT CATCACCACG CAGGCCTGTG CAGGTGTACA GATCTCGACT GGACAGTGAT
    TGCAGCCAAT CCTCACTTAG ACCGAAAGTC AGCGCCGGTT TGTCTCCTAC ATACACGACG
    GCCACGCTGG GACGAAACAG TCCTGCCAGG AGTCCTCTGA TGGTGGCCAA CAGCACCGGA
    AGTCTCCCAC GCAACCTGGC AGCCACCCTG CAGGACATCG AGACCAAGAG ACAGCTGGCT
    CTTCAGCAGA AAGGTCAGCA GGTTATCGAG GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG
    CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG TGGGAGGCGC TGCACGGCTC TCAAACGCAG
    CTCCACCAGC CCTTCTCATC AAGCCCAGCA CCGGCGCTTC ACCACTCCAT CCTGCACCAC
    ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC GACACGCTAA GCCTGGAGAG CTCGGACAGC
    ATGGACACCA GCATCTCTAC TGGAAACAAC TCGGCCTGCT CGCCGGACAA CATGTCCAGT
    GCCAGCGGTA TGGATGCGTT GAAGATTGAA GAGATGGAGA AGATGTTGAA GGAGGCGCAG
    CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA GAGCGAGAGA CTCAGAGCCG TAAACTGATG
    CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG GCCGAGAAGA GGCTGCAGGA GGAGACGCTG
    CACAGACAGC AGCTCATCGA GAAGGAGGTC AAGATGAGGG CCAGAAACTT CTCTCAGGCC
    CGTCCGATGA CCCGCTACCT GCCCATCCGC AAAGAGGAGT TTGACCTGCG TTCGCACATC
    GAGTCTTCAG GCCACAGCGT AGACACCTGC TACCACGTCA TCCTCACCGA GAAGATGTGC
    AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG ATCAAATCCT GGAAGAAGCG CTGGTTCGTC
    TTTGATCGCC TCAAAAGGAC GTTCTCTTAC TACGCCGACA AACACGAGAG CAAGCTGAAG
    GGTGTGATTT ACTTCCAGGC CATCGAGGAG GTTTACTACG ATCACCTGCG CAGCGCCACA
    AAGAGCCCGA CCCCGACGCT GACCTTCTGT GTTAAAACAC ACGACAGACT GTATTACATG
    GTGGCTCCGT CCGCAGAGGC CATGAGGATA TGGATGGATG TGATTGTGAC AGGAGCCGAG
    GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322047.1
    CDS473..5116
    Translation

    Target ORF information:

    RefSeq Version XM_021466372.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAAGCTGA GACTCTTGAA GCAGCGACAA AGCAGTTTGA AGACCTGGAG
    TTTCAGCAGT TGGAGAGGGA GAGCAGTCTG GAGGAGGAGC GCGAGACCAT CAGCCAGCAG
    CTCATGCAGG ACAGAGCTCA ATACCACAGC AGTGTCGCCA AGAGAAAGGA AAAGGTGGTG
    GCATTGGAGA GCCAGGCGAG CCAGCTGGGA ATACAGGCCG CTCAGGAGTG TGAGAGACTG
    GCCAAGGACA GAACCATCAC CTTGCAGATG CTACACAAGG AAAAGGAAAG GCTGGCCAGT
    CTGGAGAGAA GATACCAGAG TTTAACTGGT GGAAAGACGT TCCCCAAAAG CTCCAATACT
    ATAAAGGAGG AGTACTTGAA GCTGTCGGAT GTGTATAAGA TGTATGGGAG TGAATGCCAT
    GACATCCAGC TCGCCAGCGC TTCCTCACAC GGCCTCTCGC TTGCCCTTGA CCCAGCTGAA
    ACTGCCCCGC GTGAGGAGTA TGTGACGGTC AGCCAATTAA ACCAGATGTA CGGGATGCCA
    AAGGTGGAGT CTTCCCCTAC CTCACCCGTC CAATCAGTGC AGCCCTCTCT CGCAGACTCC
    ACCTTCCCCC GCCTCCCTTC CCCTCATGGC TCCTCCCTCT CTCTCTCCTT CGAGCGTCAG
    TCAGACTGGG GCAGGCCTCA GATGCCCGTG CTAGATTTAG AGCACTGGTA CCAGGAGGTA
    ATGGCAGCTG GAGATGTAAA CCAGCTCTGC CCTCCTCCAT TGCCGGCTAA AGCTTTCTCA
    TCATCATCAT CATCACCACG CAGGCCTGTG CAGGTGTACA GATCTCGACT GGACAGTGAT
    TGCAGCCAAT CCTCACTTAG ACCGAAAGTC AGCGCCGGTT TGTCTCCTAC ATACACGACG
    GCCACGCTGG GACGAAACAG TCCTGCCAGG AGTCCTCTGA TGGTGGCCAA CAGCACCGGA
    AGTCTCCCAC GCAACCTGGC AGCCACCCTG CAGGACATCG AGACCAAGAG ACAGCTGGCT
    CTTCAGCAGA AAGGTCAGCA GGTTATCGAG GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG
    CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG TGGGAGGCGC TGCACGGCTC TCAAACGCAG
    CTCCACCAGC CCTTCTCATC AAGCCCAGCA CCGGCGCTTC ACCACTCCAT CCTGCACCAC
    ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC GACACGCTAA GCCTGGAGAG CTCGGACAGC
    ATGGACACCA GCATCTCTAC TGGAAACAAC TCGGCCTGCT CGCCGGACAA CATGTCCAGT
    GCCAGCGGTA TGGATGCGTT GAAGATTGAA GAGATGGAGA AGATGTTGAA GGAGGCGCAG
    CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA GAGCGAGAGA CTCAGAGCCG TAAACTGATG
    CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG GCCGAGAAGA GGCTGCAGGA GGAGACGCTG
    CACAGACAGC AGCTCATCGA GAAGGAGGTC AAGATGAGGG CCAGAAACTT CTCTCAGGCC
    CGTCCGATGA CCCGCTACCT GCCCATCCGC AAAGAGGAGT TTGACCTGCG TTCGCACATC
    GAGTCTTCAG GCCACAGCGT AGACACCTGC TACCACGTCA TCCTCACCGA GAAGATGTGC
    AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG ATCAAATCCT GGAAGAAGCG CTGGTTCGTC
    TTTGATCGCC TCAAAAGGAC GTTCTCTTAC TACGCCGACA AACACGAGAG CAAGCTGAAG
    GGTGTGATTT ACTTCCAGGC CATCGAGGAG GTTTACTACG ATCACCTGCG CAGCGCCACA
    AAGAGCCCGA CCCCGACGCT GACCTTCTGT GTTAAAACAC ACGACAGACT GTATTACATG
    GTGGCTCCGT CCGCAGAGGC CATGAGGATA TGGATGGATG TGATTGTGAC AGGAGCCGAG
    GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149051
    Clone ID Related Accession (Same CDS sequence) XM_021466373.1
    Accession Version XM_021466373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGCGTCAGT CAGACTGGGG CAGGCCTCAG ATGCCCGTGC TAGATTTAGA GCACTGGTAC
    CAGGAGGTAA TGGCAGCTGG AGATGTAAAC CAGCTCTGCC CTCCTCCATT GCCGGCTAAA
    GCTTTCTCAT CATCATCATC ATCACCACGC AGGCCTGTGC AGGTGTACAG ATCTCGACTG
    GACAGTGATT GCAGCCAATC CTCACTTAGA CCGAAAGTCA GCGCCGGTTT GTCTCCTACA
    TACACGACGG CCACGCTGGG ACGAAACAGT CCTGCCAGGA GTCCTCTGAT GGTGGCCAAC
    AGCACCGGAA GTCTCCCACG CAACCTGGCA GCCACCCTGC AGGACATCGA GACCAAGAGA
    CAGCTGGCTC TTCAGCAGAA AGGTCAGCAG GTTATCGAGG AGCAGCGCAG GCGTCTGGCG
    GAGCTGAAGC AGAAAGCTGC AGTGGAGGCT CAGTGCCAGT GGGAGGCGCT GCACGGCTCT
    CAAACGCAGC TCCACCAGCC CTTCTCATCA AGCCCAGCAC CGGCGCTTCA CCACTCCATC
    CTGCACCACA CAGCGCCCCC TTGCGGAGAG CAGCCGTACG ACACGCTAAG CCTGGAGAGC
    TCGGACAGCA TGGACACCAG CATCTCTACT GGAAACAACT CGGCCTGCTC GCCGGACAAC
    ATGTCCAGTG CCAGCGGTAT GGATGCGTTG AAGATTGAAG AGATGGAGAA GATGTTGAAG
    GAGGCGCAGC TGGAAAAAGC TCGGCTCATA GAGTCTAGAG AGCGAGAGAC TCAGAGCCGT
    AAACTGATGC TGGAGGAGGA GCGGAGGAGG AGAGAGGAGG CCGAGAAGAG GCTGCAGGAG
    GAGACGCTGC ACAGACAGCA GCTCATCGAG AAGGAGGTCA AGATGAGGGC CAGAAACTTC
    TCTCAGGCCC GTCCGATGAC CCGCTACCTG CCCATCCGCA AAGAGGAGTT TGACCTGCGT
    TCGCACATCG AGTCTTCAGG CCACAGCGTA GACACCTGCT ACCACGTCAT CCTCACCGAG
    AAGATGTGCA AGGGCTTTCT GGTCAAGATG GGAGGCAAGA TCAAATCCTG GAAGAAGCGC
    TGGTTCGTCT TTGATCGCCT CAAAAGGACG TTCTCTTACT ACGCCGACAA ACACGAGAGC
    AAGCTGAAGG GTGTGATTTA CTTCCAGGCC ATCGAGGAGG TTTACTACGA TCACCTGCGC
    AGCGCCACAA AGAGCCCGAC CCCGACGCTG ACCTTCTGTG TTAAAACACA CGACAGACTG
    TATTACATGG TGGCTCCGTC CGCAGAGGCC ATGAGGATAT GGATGGATGT GATTGTGACA
    GGAGCCGAGG GCTACACACA GTTCATGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322048.1
    CDS472..5244
    Translation

    Target ORF information:

    RefSeq Version XM_021466373.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGCGTCAGT CAGACTGGGG CAGGCCTCAG ATGCCCGTGC TAGATTTAGA GCACTGGTAC
    CAGGAGGTAA TGGCAGCTGG AGATGTAAAC CAGCTCTGCC CTCCTCCATT GCCGGCTAAA
    GCTTTCTCAT CATCATCATC ATCACCACGC AGGCCTGTGC AGGTGTACAG ATCTCGACTG
    GACAGTGATT GCAGCCAATC CTCACTTAGA CCGAAAGTCA GCGCCGGTTT GTCTCCTACA
    TACACGACGG CCACGCTGGG ACGAAACAGT CCTGCCAGGA GTCCTCTGAT GGTGGCCAAC
    AGCACCGGAA GTCTCCCACG CAACCTGGCA GCCACCCTGC AGGACATCGA GACCAAGAGA
    CAGCTGGCTC TTCAGCAGAA AGGTCAGCAG GTTATCGAGG AGCAGCGCAG GCGTCTGGCG
    GAGCTGAAGC AGAAAGCTGC AGTGGAGGCT CAGTGCCAGT GGGAGGCGCT GCACGGCTCT
    CAAACGCAGC TCCACCAGCC CTTCTCATCA AGCCCAGCAC CGGCGCTTCA CCACTCCATC
    CTGCACCACA CAGCGCCCCC TTGCGGAGAG CAGCCGTACG ACACGCTAAG CCTGGAGAGC
    TCGGACAGCA TGGACACCAG CATCTCTACT GGAAACAACT CGGCCTGCTC GCCGGACAAC
    ATGTCCAGTG CCAGCGGTAT GGATGCGTTG AAGATTGAAG AGATGGAGAA GATGTTGAAG
    GAGGCGCAGC TGGAAAAAGC TCGGCTCATA GAGTCTAGAG AGCGAGAGAC TCAGAGCCGT
    AAACTGATGC TGGAGGAGGA GCGGAGGAGG AGAGAGGAGG CCGAGAAGAG GCTGCAGGAG
    GAGACGCTGC ACAGACAGCA GCTCATCGAG AAGGAGGTCA AGATGAGGGC CAGAAACTTC
    TCTCAGGCCC GTCCGATGAC CCGCTACCTG CCCATCCGCA AAGAGGAGTT TGACCTGCGT
    TCGCACATCG AGTCTTCAGG CCACAGCGTA GACACCTGCT ACCACGTCAT CCTCACCGAG
    AAGATGTGCA AGGGCTTTCT GGTCAAGATG GGAGGCAAGA TCAAATCCTG GAAGAAGCGC
    TGGTTCGTCT TTGATCGCCT CAAAAGGACG TTCTCTTACT ACGCCGACAA ACACGAGAGC
    AAGCTGAAGG GTGTGATTTA CTTCCAGGCC ATCGAGGAGG TTTACTACGA TCACCTGCGC
    AGCGCCACAA AGAGCCCGAC CCCGACGCTG ACCTTCTGTG TTAAAACACA CGACAGACTG
    TATTACATGG TGGCTCCGTC CGCAGAGGCC ATGAGGATAT GGATGGATGT GATTGTGACA
    GGAGCCGAGG GCTACACACA GTTCATGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45936
    Clone ID Related Accession (Same CDS sequence) XM_021466359.1
    Accession Version XM_021466359.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGCATGCCA TTCTTCCAGA TGGCCACTCA AGGCCCATTC AGGTCTCGAT
    TGCCATCCTC AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGCGTCAGT CAGACTGGGG CAGGCCTCAG ATGCCCGTGC TAGATTTAGA GCACTGGTAC
    CAGGAGGTAA TGGCAGCTGG AGATGTAAAC CAGCTCTGCC CTCCTCCATT GCCGGCTAAA
    GCTTTCTCAT CATCATCATC ATCACCACGC AGGCCTGTGC AGGTGTACAG ATCTCGACTG
    GACAGTGATT GCAGCCAATC CTCACTTAGA CCGAAAGTCA GCGCCGGTTT GTCTCCTACA
    TACACGACGG CCACGCTGGG ACGAAACAGT CCTGCCAGGA GTCCTCTGAT GGTGGCCAAC
    AGCACCGGAA GTCTCCCACG CAACCTGGCA GCCACCCTGC AGGACATCGA GACCAAGAGA
    CAGCTGGCTC TTCAGCAGAA AGGTCAGCAG GTTATCGAGG AGCAGCGCAG GCGTCTGGCG
    GAGCTGAAGC AGAAAGCTGC AGTGGAGGCT CAGTGCCAGT GGGAGGCGCT GCACGGCTCT
    CAAACGCAGC TCCACCAGCC CTTCTCATCA AGCCCAGCAC CGGCGCTTCA CCACTCCATC
    CTGCACCACA CAGCGCCCCC TTGCGGAGAG CAGCCGTACG ACACGCTAAG CCTGGAGAGC
    TCGGACAGCA TGGACACCAG CATCTCTACT GGAAACAACT CGGCCTGCTC GCCGGACAAC
    ATGTCCAGTG CCAGCGGTAT GGATGCGTTG AAGATTGAAG AGATGGAGAA GATGTTGAAG
    GAGGCGCAGC TGGAAAAAGC TCGGCTCATA GAGTCTAGAG AGCGAGAGAC TCAGAGCCGT
    AAACTGATGC TGGAGGAGGA GCGGAGGAGG AGAGAGGAGG CCGAGAAGAG GCTGCAGGAG
    GAGACGCTGC ACAGACAGCA GCTCATCGAG AAGGAGGTCA AGATGAGGGC CAGAAACTTC
    TCTCAGGCCC GTCCGATGAC CCGCTACCTG CCCATCCGCA AAGAGGAGTT TGACCTGCGT
    TCGCACATCG AGTCTTCAGG CCACAGCGTA GACACCTGCT ACCACGTCAT CCTCACCGAG
    AAGATGTGCA AGGGCTTTCT GGTCAAGATG GGAGGCAAGA TCAAATCCTG GAAGAAGCGC
    TGGTTCGTCT TTGATCGCCT CAAAAGGACG TTCTCTTACT ACGCCGACAA ACACGAGAGC
    AAGCTGAAGG GTGTGATTTA CTTCCAGGCC ATCGAGGAGG TTTACTACGA TCACCTGCGC
    AGCGCCACAA AGAAAGGATT TTTCAATCTG AATCTGACAA ACAGCCCGAC CCCGACGCTG
    ACCTTCTGTG TTAAAACACA CGACAGACTG TATTACATGG TGGCTCCGTC CGCAGAGGCC
    ATGAGGATAT GGATGGATGT GATTGTGACA GGAGCCGAGG GCTACACACA GTTCATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322034.1
    CDS474..5336
    Translation

    Target ORF information:

    RefSeq Version XM_021466359.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466359.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGCATGCCA TTCTTCCAGA TGGCCACTCA AGGCCCATTC AGGTCTCGAT
    TGCCATCCTC AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGCGTCAGT CAGACTGGGG CAGGCCTCAG ATGCCCGTGC TAGATTTAGA GCACTGGTAC
    CAGGAGGTAA TGGCAGCTGG AGATGTAAAC CAGCTCTGCC CTCCTCCATT GCCGGCTAAA
    GCTTTCTCAT CATCATCATC ATCACCACGC AGGCCTGTGC AGGTGTACAG ATCTCGACTG
    GACAGTGATT GCAGCCAATC CTCACTTAGA CCGAAAGTCA GCGCCGGTTT GTCTCCTACA
    TACACGACGG CCACGCTGGG ACGAAACAGT CCTGCCAGGA GTCCTCTGAT GGTGGCCAAC
    AGCACCGGAA GTCTCCCACG CAACCTGGCA GCCACCCTGC AGGACATCGA GACCAAGAGA
    CAGCTGGCTC TTCAGCAGAA AGGTCAGCAG GTTATCGAGG AGCAGCGCAG GCGTCTGGCG
    GAGCTGAAGC AGAAAGCTGC AGTGGAGGCT CAGTGCCAGT GGGAGGCGCT GCACGGCTCT
    CAAACGCAGC TCCACCAGCC CTTCTCATCA AGCCCAGCAC CGGCGCTTCA CCACTCCATC
    CTGCACCACA CAGCGCCCCC TTGCGGAGAG CAGCCGTACG ACACGCTAAG CCTGGAGAGC
    TCGGACAGCA TGGACACCAG CATCTCTACT GGAAACAACT CGGCCTGCTC GCCGGACAAC
    ATGTCCAGTG CCAGCGGTAT GGATGCGTTG AAGATTGAAG AGATGGAGAA GATGTTGAAG
    GAGGCGCAGC TGGAAAAAGC TCGGCTCATA GAGTCTAGAG AGCGAGAGAC TCAGAGCCGT
    AAACTGATGC TGGAGGAGGA GCGGAGGAGG AGAGAGGAGG CCGAGAAGAG GCTGCAGGAG
    GAGACGCTGC ACAGACAGCA GCTCATCGAG AAGGAGGTCA AGATGAGGGC CAGAAACTTC
    TCTCAGGCCC GTCCGATGAC CCGCTACCTG CCCATCCGCA AAGAGGAGTT TGACCTGCGT
    TCGCACATCG AGTCTTCAGG CCACAGCGTA GACACCTGCT ACCACGTCAT CCTCACCGAG
    AAGATGTGCA AGGGCTTTCT GGTCAAGATG GGAGGCAAGA TCAAATCCTG GAAGAAGCGC
    TGGTTCGTCT TTGATCGCCT CAAAAGGACG TTCTCTTACT ACGCCGACAA ACACGAGAGC
    AAGCTGAAGG GTGTGATTTA CTTCCAGGCC ATCGAGGAGG TTTACTACGA TCACCTGCGC
    AGCGCCACAA AGAAAGGATT TTTCAATCTG AATCTGACAA ACAGCCCGAC CCCGACGCTG
    ACCTTCTGTG TTAAAACACA CGACAGACTG TATTACATGG TGGCTCCGTC CGCAGAGGCC
    ATGAGGATAT GGATGGATGT GATTGTGACA GGAGCCGAGG GCTACACACA GTTCATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45937
    Clone ID Related Accession (Same CDS sequence) XM_021466361.1
    Accession Version XM_021466361.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4836bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGGCGT TGTACTTATT CGACAACTGG AAAAAGGATT CTCATGTCCC CGAGCAAGTA 
    AGTGACAGTA CACTGGCCAT GGATACTTTG AACCAAAATC TTAGCCATGG GCATCAGTCT
    CATCAGGTGC TCCAGAGTAC TCCACTGGAT CTTATTGAGA CTGGAAAGGC ACTGAAGGTT
    CAAGCAGAGC GCCCCCATTT AGTCAGTCTG GGAAGTGGGC GTCTCAGTAC AGCCATCACC
    CTCTTACCCC TGCCTGAAGG GAAGACGACT GTGGGTCATG GAGCCATGGA CATCAGCATA
    CAGGGCCCCG GGGTCGCAGC TCAGCACTGC TTTATTGAGA ACAGGGCTGG AATCATCACA
    CTTCACCCCT GTGGAAACCA GTGCAGCATG GACGGACTGC CGGTCAACAA ACCTGTGCGA
    CTCTCTCAAG GCTGCATGCT GTGTTTCGGC CAATCCGCCT TCTTCCGCTT CAACCACCCC
    GAGGAGGCTT TCCGAATGAA AAGCATGATG CCCGAGGGAG GACAGACAGC CAGTGTGAAA
    AACTGGACCC ACAAAGATTC AGAGAACCTG GTCAATGGGA ACCACTCTTC AGGAGAGGCA
    AAATCCCACT TGGTTTCCAA CGAGAGAGTG CGCCCTGAGC ACAGTGCCAT CGTCAGTTCC
    ATTGAGAAGG ACCTTCAGGA CATTATGGAC TCATTAGTCA TGGATGACCC TCAGCCCTCA
    TCCTCCGAGT CAAAGAAGCC CTCTAGTCAG TCCATCTCAC AGTCTCCCCT TTCTCCGATG
    TTAAATGGAG GGGGGCGCTT CCTTCTCTCT CCCCCAACGA GCCCTGGTGC TATGTCTGTG
    GGCTCCAGTT ATGAGAATAA CTCCCCTCCT TTCTCTCCTC TGTCATCTCC CTCGGCTGCC
    AGCAGTGGCA GCTACACCAC TCTCTCACCA ATTGGTTGTC AGGACCAGCC TCACATGCTT
    CCTCCTGTGC CAGTGAGGTC TTCTAGTTAC AATTATACCA GCCAGCCACC CATACCTCAG
    CCACGGACCA TACTGCCCAG CTATTCCAGT GGCAGCAGTG GGCCAAAGGT GCCTGAGAGT
    CCCAGACTCC AGCGAAAGGC GCTTTTAGAA GCACCGCCAA GCCCAAAGCC AAGCCGTAGG
    GGGTTGAACC AAGACAGCTC GGTGGCTAAA ACCCCAGACA GCCCCATACA GACTCACATT
    CTGCCCTCAG TCTCCATCTC TACAGCTCCG ACTGATGTCC CATCTGTTAG TCGGGTACCA
    GTTCCAGGTA GTCCTAGATT AGCCACCAAA TTCTCCACTG CGTCCTCACC TGCCTCACCC
    TCTAGTCCCA GAACCAAAAC TACCATTGTC CTCCAAGATC GACCCTCAAG TCCTTTCCGG
    GAGCAACCTC AGCCAGATCG TTCTTTGACG TCCAGCCCCT CTCACCAACT CTCCCCACCA
    TCAAGATCTT TCCAGCCCCC ATTGGACCCC ATTGTTCACA TCATTCAAGG TGGACCTTCA
    CACCCACATC CTCGAACACT GCAGCCTCCA GAAAGTCCGC GGCTGGCCCG CAGAAATCTT
    GAAGGGAGCA GCATGAGGGA ACTCCCCCCT CTCAGTCCCT CCATAGCTCG CAGGGGGGTT
    CCTGTGCTCC CAGGTGCTCT ACCAGGAACC CTGCGAACTC CAGAGAGCCC ATCCCCAAGA
    ATGGTGCCAG AGAGTCCCAG ACTCAGGAGG AAAACAGGGT CACCTACAGA GGAGCCATTC
    AGTCCTCGTG CGATGCGCAC TCGTAGTCCT TCGCCCACCT CTGGACTGAC TGGCGAAAGC
    AGTGGACGGA AGGGAAGTTT TGGGAACTCA CTCTCTTCTG CCTTCAGCCT AAGTTCTCTG
    CCTGGGTCTT CCCCACGATC TAGTCCCAGG AGCCACCGAA AGATGTCAGC AGGCCACAGA
    GACCTCCGGA TGCCTCACCC AGGCATGAGG GAGCGCAAAA ACAGCATCAC AGAGATCAGC
    GACAACGAGG ATGACCTTCT GGAGTACCAT CGACGCCAAA GAGAAGAGCG ACTCAGAGAG
    CAGGAAATGG AGAGACTGGA ACGGCAGCGG CTGGAGACAA TCTTGAACCT CTGCGCTGAG
    TATAACAAGG GCGACGGGCC GCCGGGTGAT TTGGGCAGGA TGGGCGTCTC TGGGCTTGGC
    TCGAGGGACG GGCTTAACGT CAGGCGTCCT TCAATGGACA GCGTGAACAG CGCTTCACTA
    AGAGCGACTC AAAGACAACG GGAAAGCCAA GAGGACAACC TGAAGGAGGA TAGCAGTAGC
    ACTGAGAGTG CGCAGCTGGA CGGACGGACA CCTGCTCTGC TAAATGGACA GAATGGACAG
    GCTGAGGAGT CTCTGGGTTC GGGCAGCATT GGCCGTCTGG AGCTGGGCTA CCTGGAGGAG
    GAGCGGGTTC GAGTGCTGGC AAAGGTAGAT GAGCTCAAAG CTCGAATCAC AGAACTGGAG
    CAGCAACTTC AGGAGTCTAA ACAAGAGGCG GAGATGGAGA GAGCTCTGCT GCAGGGAGAA
    AGACAGGCTG AGATGGAGCA AATCGAGACT GAGACCGAGA TTATCAACCA GCTGCAGCGC
    AAACTCACTG AACTGGAGAG CAGCATCCAG AGAGAGAAGG ACAAGGCATG CCATTCTTCC
    AGATGGCCAC TCAAGGCCCA TTCAGGTCTC GATTGCCATC CTCAGGAGAG GGCTAATGTT
    GACGCGGAGC GGGAAGCCCT GGAGAGGCTT CAGGATGGGT ACAATGAGTT GACGAGCCAG
    CTACATAACT GCCCTGAGTC TCTGAGGGAA CAGTTACAGG AACAGCTCAA GAGGGAAGCT
    GAGACTCTTG AAGCAGCGAC AAAGCAGTTT GAAGACCTGG AGTTTCAGCA GTTGGAGAGG
    GAGAGCAGTC TGGAGGAGGA GCGCGAGACC ATCAGCCAGC AGCTCATGCA GGACAGAGCT
    CAATACCACA GCAGTGTCGC CAAGAGAAAG GAAAAGGTGG TGGCATTGGA GAGCCAGGCG
    AGCCAGCTGG GAATACAGGC CGCTCAGGAG TGTGAGAGAC TGGCCAAGGA CAGAACCATC
    ACCTTGCAGA TGCTACACAA GGAAAAGGAA AGGCTGGCCA GTCTGGAGAG AAGATACCAG
    AGTTTAACTG GTGGAAAGAC GTTCCCCAAA AGCTCCAATA CTATAAAGGA GGAGTACTTG
    AAGCTGTCGG ATGTGTATAA GATGTATGGG AGTGAATGCC ATGACATCCA GCTCGCCAGC
    GCTTCCTCAC ACGGCCTCTC GCTTGCCCTT GACCCAGCTG AAACTGCCCC GCGTGAGGAG
    TATGTGACGG TCAGCCAATT AAACCAGATG TACGGGATGC CAAAGGTGGA GTCTTCCCCT
    ACCTCACCCG TCCAATCAGT GCAGCCCTCT CTCGCAGACT CCACCTTCCC CCGCCTCCCT
    TCCCCTCATG GCTCCTCCCT CTCTCTCTCC TTCGAGCGTC AGTCAGACTG GGGCAGGCCT
    CAGATGCCCG TGCTAGATTT AGAGCACTGG TACCAGGAGG TAATGGCAGC TGGAGATGTA
    AACCAGCTCT GCCCTCCTCC ATTGCCGGCT AAAGCTTTCT CATCATCATC ATCATCACCA
    CGCAGGCCTG TGCAGGTGTA CAGATCTCGA CTGGACAGTG ATTGCAGCCA ATCCTCACTT
    AGACCGAAAG TCAGCGCCGG TTTGTCTCCT ACATACACGA CGGCCACGCT GGGACGAAAC
    AGTCCTGCCA GGAGTCCTCT GATGGTGGCC AACAGCACCG GAAGTCTCCC ACGCAACCTG
    GCAGCCACCC TGCAGGACAT CGAGACCAAG AGACAGCTGG CTCTTCAGCA GAAAGGTCAG
    CAGGTTATCG AGGAGCAGCG CAGGCGTCTG GCGGAGCTGA AGCAGAAAGC TGCAGTGGAG
    GCTCAGTGCC AGTGGGAGGC GCTGCACGGC TCTCAAACGC AGCTCCACCA GCCCTTCTCA
    TCAAGCCCAG CACCGGCGCT TCACCACTCC ATCCTGCACC ACACAGCGCC CCCTTGCGGA
    GAGCAGCCGT ACGACACGCT AAGCCTGGAG AGCTCGGACA GCATGGACAC CAGCATCTCT
    ACTGGAAACA ACTCGGCCTG CTCGCCGGAC AACATGTCCA GTGCCAGCGG TATGGATGCG
    TTGAAGATTG AAGAGATGGA GAAGATGTTG AAGGAGGCGC AGCTGGAAAA AGCTCGGCTC
    ATAGAGTCTA GAGAGCGAGA GACTCAGAGC CGTAAACTGA TGCTGGAGGA GGAGCGGAGG
    AGGAGAGAGG AGGCCGAGAA GAGGCTGCAG GAGGAGACGC TGCACAGACA GCAGCTCATC
    GAGAAGGAGG TCAAGATGAG GGCCAGAAAC TTCTCTCAGG CCCGTCCGAT GACCCGCTAC
    CTGCCCATCC GCAAAGAGGA GTTTGACCTG CGTTCGCACA TCGAGTCTTC AGGCCACAGC
    GTAGACACCT GCTACCACGT CATCCTCACC GAGAAGATGT GCAAGGGCTT TCTGGTCAAG
    ATGGGAGGCA AGATCAAATC CTGGAAGAAG CGCTGGTTCG TCTTTGATCG CCTCAAAAGG
    ACGTTCTCTT ACTACGCCGA CAAACACGAG AGCAAGCTGA AGGGTGTGAT TTACTTCCAG
    GCCATCGAGG AGGTTTACTA CGATCACCTG CGCAGCGCCA CAAAGAAAGG ATTTTTCAAT
    CTGAATCTGA CAAACAGCCC GACCCCGACG CTGACCTTCT GTGTTAAAAC ACACGACAGA
    CTGTATTACA TGGTGGCTCC GTCCGCAGAG GCCATGAGGA TATGGATGGA TGTGATTGTG
    ACAGGAGCCG AGGGCTACAC ACAGTTCATG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322036.1
    CDS84..4919
    Translation

    Target ORF information:

    RefSeq Version XM_021466361.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466361.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGGCGT TGTACTTATT CGACAACTGG AAAAAGGATT CTCATGTCCC CGAGCAAGTA 
    AGTGACAGTA CACTGGCCAT GGATACTTTG AACCAAAATC TTAGCCATGG GCATCAGTCT
    CATCAGGTGC TCCAGAGTAC TCCACTGGAT CTTATTGAGA CTGGAAAGGC ACTGAAGGTT
    CAAGCAGAGC GCCCCCATTT AGTCAGTCTG GGAAGTGGGC GTCTCAGTAC AGCCATCACC
    CTCTTACCCC TGCCTGAAGG GAAGACGACT GTGGGTCATG GAGCCATGGA CATCAGCATA
    CAGGGCCCCG GGGTCGCAGC TCAGCACTGC TTTATTGAGA ACAGGGCTGG AATCATCACA
    CTTCACCCCT GTGGAAACCA GTGCAGCATG GACGGACTGC CGGTCAACAA ACCTGTGCGA
    CTCTCTCAAG GCTGCATGCT GTGTTTCGGC CAATCCGCCT TCTTCCGCTT CAACCACCCC
    GAGGAGGCTT TCCGAATGAA AAGCATGATG CCCGAGGGAG GACAGACAGC CAGTGTGAAA
    AACTGGACCC ACAAAGATTC AGAGAACCTG GTCAATGGGA ACCACTCTTC AGGAGAGGCA
    AAATCCCACT TGGTTTCCAA CGAGAGAGTG CGCCCTGAGC ACAGTGCCAT CGTCAGTTCC
    ATTGAGAAGG ACCTTCAGGA CATTATGGAC TCATTAGTCA TGGATGACCC TCAGCCCTCA
    TCCTCCGAGT CAAAGAAGCC CTCTAGTCAG TCCATCTCAC AGTCTCCCCT TTCTCCGATG
    TTAAATGGAG GGGGGCGCTT CCTTCTCTCT CCCCCAACGA GCCCTGGTGC TATGTCTGTG
    GGCTCCAGTT ATGAGAATAA CTCCCCTCCT TTCTCTCCTC TGTCATCTCC CTCGGCTGCC
    AGCAGTGGCA GCTACACCAC TCTCTCACCA ATTGGTTGTC AGGACCAGCC TCACATGCTT
    CCTCCTGTGC CAGTGAGGTC TTCTAGTTAC AATTATACCA GCCAGCCACC CATACCTCAG
    CCACGGACCA TACTGCCCAG CTATTCCAGT GGCAGCAGTG GGCCAAAGGT GCCTGAGAGT
    CCCAGACTCC AGCGAAAGGC GCTTTTAGAA GCACCGCCAA GCCCAAAGCC AAGCCGTAGG
    GGGTTGAACC AAGACAGCTC GGTGGCTAAA ACCCCAGACA GCCCCATACA GACTCACATT
    CTGCCCTCAG TCTCCATCTC TACAGCTCCG ACTGATGTCC CATCTGTTAG TCGGGTACCA
    GTTCCAGGTA GTCCTAGATT AGCCACCAAA TTCTCCACTG CGTCCTCACC TGCCTCACCC
    TCTAGTCCCA GAACCAAAAC TACCATTGTC CTCCAAGATC GACCCTCAAG TCCTTTCCGG
    GAGCAACCTC AGCCAGATCG TTCTTTGACG TCCAGCCCCT CTCACCAACT CTCCCCACCA
    TCAAGATCTT TCCAGCCCCC ATTGGACCCC ATTGTTCACA TCATTCAAGG TGGACCTTCA
    CACCCACATC CTCGAACACT GCAGCCTCCA GAAAGTCCGC GGCTGGCCCG CAGAAATCTT
    GAAGGGAGCA GCATGAGGGA ACTCCCCCCT CTCAGTCCCT CCATAGCTCG CAGGGGGGTT
    CCTGTGCTCC CAGGTGCTCT ACCAGGAACC CTGCGAACTC CAGAGAGCCC ATCCCCAAGA
    ATGGTGCCAG AGAGTCCCAG ACTCAGGAGG AAAACAGGGT CACCTACAGA GGAGCCATTC
    AGTCCTCGTG CGATGCGCAC TCGTAGTCCT TCGCCCACCT CTGGACTGAC TGGCGAAAGC
    AGTGGACGGA AGGGAAGTTT TGGGAACTCA CTCTCTTCTG CCTTCAGCCT AAGTTCTCTG
    CCTGGGTCTT CCCCACGATC TAGTCCCAGG AGCCACCGAA AGATGTCAGC AGGCCACAGA
    GACCTCCGGA TGCCTCACCC AGGCATGAGG GAGCGCAAAA ACAGCATCAC AGAGATCAGC
    GACAACGAGG ATGACCTTCT GGAGTACCAT CGACGCCAAA GAGAAGAGCG ACTCAGAGAG
    CAGGAAATGG AGAGACTGGA ACGGCAGCGG CTGGAGACAA TCTTGAACCT CTGCGCTGAG
    TATAACAAGG GCGACGGGCC GCCGGGTGAT TTGGGCAGGA TGGGCGTCTC TGGGCTTGGC
    TCGAGGGACG GGCTTAACGT CAGGCGTCCT TCAATGGACA GCGTGAACAG CGCTTCACTA
    AGAGCGACTC AAAGACAACG GGAAAGCCAA GAGGACAACC TGAAGGAGGA TAGCAGTAGC
    ACTGAGAGTG CGCAGCTGGA CGGACGGACA CCTGCTCTGC TAAATGGACA GAATGGACAG
    GCTGAGGAGT CTCTGGGTTC GGGCAGCATT GGCCGTCTGG AGCTGGGCTA CCTGGAGGAG
    GAGCGGGTTC GAGTGCTGGC AAAGGTAGAT GAGCTCAAAG CTCGAATCAC AGAACTGGAG
    CAGCAACTTC AGGAGTCTAA ACAAGAGGCG GAGATGGAGA GAGCTCTGCT GCAGGGAGAA
    AGACAGGCTG AGATGGAGCA AATCGAGACT GAGACCGAGA TTATCAACCA GCTGCAGCGC
    AAACTCACTG AACTGGAGAG CAGCATCCAG AGAGAGAAGG ACAAGGCATG CCATTCTTCC
    AGATGGCCAC TCAAGGCCCA TTCAGGTCTC GATTGCCATC CTCAGGAGAG GGCTAATGTT
    GACGCGGAGC GGGAAGCCCT GGAGAGGCTT CAGGATGGGT ACAATGAGTT GACGAGCCAG
    CTACATAACT GCCCTGAGTC TCTGAGGGAA CAGTTACAGG AACAGCTCAA GAGGGAAGCT
    GAGACTCTTG AAGCAGCGAC AAAGCAGTTT GAAGACCTGG AGTTTCAGCA GTTGGAGAGG
    GAGAGCAGTC TGGAGGAGGA GCGCGAGACC ATCAGCCAGC AGCTCATGCA GGACAGAGCT
    CAATACCACA GCAGTGTCGC CAAGAGAAAG GAAAAGGTGG TGGCATTGGA GAGCCAGGCG
    AGCCAGCTGG GAATACAGGC CGCTCAGGAG TGTGAGAGAC TGGCCAAGGA CAGAACCATC
    ACCTTGCAGA TGCTACACAA GGAAAAGGAA AGGCTGGCCA GTCTGGAGAG AAGATACCAG
    AGTTTAACTG GTGGAAAGAC GTTCCCCAAA AGCTCCAATA CTATAAAGGA GGAGTACTTG
    AAGCTGTCGG ATGTGTATAA GATGTATGGG AGTGAATGCC ATGACATCCA GCTCGCCAGC
    GCTTCCTCAC ACGGCCTCTC GCTTGCCCTT GACCCAGCTG AAACTGCCCC GCGTGAGGAG
    TATGTGACGG TCAGCCAATT AAACCAGATG TACGGGATGC CAAAGGTGGA GTCTTCCCCT
    ACCTCACCCG TCCAATCAGT GCAGCCCTCT CTCGCAGACT CCACCTTCCC CCGCCTCCCT
    TCCCCTCATG GCTCCTCCCT CTCTCTCTCC TTCGAGCGTC AGTCAGACTG GGGCAGGCCT
    CAGATGCCCG TGCTAGATTT AGAGCACTGG TACCAGGAGG TAATGGCAGC TGGAGATGTA
    AACCAGCTCT GCCCTCCTCC ATTGCCGGCT AAAGCTTTCT CATCATCATC ATCATCACCA
    CGCAGGCCTG TGCAGGTGTA CAGATCTCGA CTGGACAGTG ATTGCAGCCA ATCCTCACTT
    AGACCGAAAG TCAGCGCCGG TTTGTCTCCT ACATACACGA CGGCCACGCT GGGACGAAAC
    AGTCCTGCCA GGAGTCCTCT GATGGTGGCC AACAGCACCG GAAGTCTCCC ACGCAACCTG
    GCAGCCACCC TGCAGGACAT CGAGACCAAG AGACAGCTGG CTCTTCAGCA GAAAGGTCAG
    CAGGTTATCG AGGAGCAGCG CAGGCGTCTG GCGGAGCTGA AGCAGAAAGC TGCAGTGGAG
    GCTCAGTGCC AGTGGGAGGC GCTGCACGGC TCTCAAACGC AGCTCCACCA GCCCTTCTCA
    TCAAGCCCAG CACCGGCGCT TCACCACTCC ATCCTGCACC ACACAGCGCC CCCTTGCGGA
    GAGCAGCCGT ACGACACGCT AAGCCTGGAG AGCTCGGACA GCATGGACAC CAGCATCTCT
    ACTGGAAACA ACTCGGCCTG CTCGCCGGAC AACATGTCCA GTGCCAGCGG TATGGATGCG
    TTGAAGATTG AAGAGATGGA GAAGATGTTG AAGGAGGCGC AGCTGGAAAA AGCTCGGCTC
    ATAGAGTCTA GAGAGCGAGA GACTCAGAGC CGTAAACTGA TGCTGGAGGA GGAGCGGAGG
    AGGAGAGAGG AGGCCGAGAA GAGGCTGCAG GAGGAGACGC TGCACAGACA GCAGCTCATC
    GAGAAGGAGG TCAAGATGAG GGCCAGAAAC TTCTCTCAGG CCCGTCCGAT GACCCGCTAC
    CTGCCCATCC GCAAAGAGGA GTTTGACCTG CGTTCGCACA TCGAGTCTTC AGGCCACAGC
    GTAGACACCT GCTACCACGT CATCCTCACC GAGAAGATGT GCAAGGGCTT TCTGGTCAAG
    ATGGGAGGCA AGATCAAATC CTGGAAGAAG CGCTGGTTCG TCTTTGATCG CCTCAAAAGG
    ACGTTCTCTT ACTACGCCGA CAAACACGAG AGCAAGCTGA AGGGTGTGAT TTACTTCCAG
    GCCATCGAGG AGGTTTACTA CGATCACCTG CGCAGCGCCA CAAAGAAAGG ATTTTTCAAT
    CTGAATCTGA CAAACAGCCC GACCCCGACG CTGACCTTCT GTGTTAAAAC ACACGACAGA
    CTGTATTACA TGGTGGCTCC GTCCGCAGAG GCCATGAGGA TATGGATGGA TGTGATTGTG
    ACAGGAGCCG AGGGCTACAC ACAGTTCATG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45938
    Clone ID Related Accession (Same CDS sequence) XM_021466362.1
    Accession Version XM_021466362.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGCATGCCA TTCTTCCAGA TGGCCACTCA AGGCCCATTC AGGTCTCGAT
    TGCCATCCTC AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGCGTCAGT CAGACTGGGG CAGGCCTCAG ATGCCCGTGC TAGATTTAGA GCACTGGTAC
    CAGGAGGTAA TGGCAGCTGG AGATGTAAAC CAGCTCTGCC CTCCTCCATT GCCGGCTAAA
    GCTTTCTCAT CATCATCATC ATCACCACGC AGGCCTGTGC AGGTGTACAG ATCTCGACTG
    GACAGTGATT GCAGCCAATC CTCACTTAGA CCGAAAGTCA GCGCCGGTTT GTCTCCTACA
    TACACGACGG CCACGCTGGG ACGAAACAGT CCTGCCAGGA GTCCTCTGAT GGTGGCCAAC
    AGCACCGGAA GTCTCCCACG CAACCTGGCA GCCACCCTGC AGGACATCGA GACCAAGAGA
    CAGCTGGCTC TTCAGCAGAA AGGTCAGCAG GTTATCGAGG AGCAGCGCAG GCGTCTGGCG
    GAGCTGAAGC AGAAAGCTGC AGTGGAGGCT CAGTGCCAGT GGGAGGCGCT GCACGGCTCT
    CAAACGCAGC TCCACCAGCC CTTCTCATCA AGCCCAGCAC CGGCGCTTCA CCACTCCATC
    CTGCACCACA CAGCGCCCCC TTGCGGAGAG CAGCCGTACG ACACGCTAAG CCTGGAGAGC
    TCGGACAGCA TGGACACCAG CATCTCTACT GGAAACAACT CGGCCTGCTC GCCGGACAAC
    ATGTCCAGTG CCAGCGGTAT GGATGCGTTG AAGATTGAAG AGATGGAGAA GATGTTGAAG
    GAGGCGCAGC TGGAAAAAGC TCGGCTCATA GAGTCTAGAG AGCGAGAGAC TCAGAGCCGT
    AAACTGATGC TGGAGGAGGA GCGGAGGAGG AGAGAGGAGG CCGAGAAGAG GCTGCAGGAG
    GAGACGCTGC ACAGACAGCA GCTCATCGAG AAGGAGGTCA AGATGAGGGC CAGAAACTTC
    TCTCAGGCCC GTCCGATGAC CCGCTACCTG CCCATCCGCA AAGAGGAGTT TGACCTGCGT
    TCGCACATCG AGTCTTCAGG CCACAGCGTA GACACCTGCT ACCACGTCAT CCTCACCGAG
    AAGATGTGCA AGGGCTTTCT GGTCAAGATG GGAGGCAAGA TCAAATCCTG GAAGAAGCGC
    TGGTTCGTCT TTGATCGCCT CAAAAGGACG TTCTCTTACT ACGCCGACAA ACACGAGAGC
    AAGCTGAAGG GTGTGATTTA CTTCCAGGCC ATCGAGGAGG TTTACTACGA TCACCTGCGC
    AGCGCCACAA AGAGCCCGAC CCCGACGCTG ACCTTCTGTG TTAAAACACA CGACAGACTG
    TATTACATGG TGGCTCCGTC CGCAGAGGCC ATGAGGATAT GGATGGATGT GATTGTGACA
    GGAGCCGAGG GCTACACACA GTTCATGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322037.1
    CDS473..5305
    Translation

    Target ORF information:

    RefSeq Version XM_021466362.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466362.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGCATGCCA TTCTTCCAGA TGGCCACTCA AGGCCCATTC AGGTCTCGAT
    TGCCATCCTC AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGCGTCAGT CAGACTGGGG CAGGCCTCAG ATGCCCGTGC TAGATTTAGA GCACTGGTAC
    CAGGAGGTAA TGGCAGCTGG AGATGTAAAC CAGCTCTGCC CTCCTCCATT GCCGGCTAAA
    GCTTTCTCAT CATCATCATC ATCACCACGC AGGCCTGTGC AGGTGTACAG ATCTCGACTG
    GACAGTGATT GCAGCCAATC CTCACTTAGA CCGAAAGTCA GCGCCGGTTT GTCTCCTACA
    TACACGACGG CCACGCTGGG ACGAAACAGT CCTGCCAGGA GTCCTCTGAT GGTGGCCAAC
    AGCACCGGAA GTCTCCCACG CAACCTGGCA GCCACCCTGC AGGACATCGA GACCAAGAGA
    CAGCTGGCTC TTCAGCAGAA AGGTCAGCAG GTTATCGAGG AGCAGCGCAG GCGTCTGGCG
    GAGCTGAAGC AGAAAGCTGC AGTGGAGGCT CAGTGCCAGT GGGAGGCGCT GCACGGCTCT
    CAAACGCAGC TCCACCAGCC CTTCTCATCA AGCCCAGCAC CGGCGCTTCA CCACTCCATC
    CTGCACCACA CAGCGCCCCC TTGCGGAGAG CAGCCGTACG ACACGCTAAG CCTGGAGAGC
    TCGGACAGCA TGGACACCAG CATCTCTACT GGAAACAACT CGGCCTGCTC GCCGGACAAC
    ATGTCCAGTG CCAGCGGTAT GGATGCGTTG AAGATTGAAG AGATGGAGAA GATGTTGAAG
    GAGGCGCAGC TGGAAAAAGC TCGGCTCATA GAGTCTAGAG AGCGAGAGAC TCAGAGCCGT
    AAACTGATGC TGGAGGAGGA GCGGAGGAGG AGAGAGGAGG CCGAGAAGAG GCTGCAGGAG
    GAGACGCTGC ACAGACAGCA GCTCATCGAG AAGGAGGTCA AGATGAGGGC CAGAAACTTC
    TCTCAGGCCC GTCCGATGAC CCGCTACCTG CCCATCCGCA AAGAGGAGTT TGACCTGCGT
    TCGCACATCG AGTCTTCAGG CCACAGCGTA GACACCTGCT ACCACGTCAT CCTCACCGAG
    AAGATGTGCA AGGGCTTTCT GGTCAAGATG GGAGGCAAGA TCAAATCCTG GAAGAAGCGC
    TGGTTCGTCT TTGATCGCCT CAAAAGGACG TTCTCTTACT ACGCCGACAA ACACGAGAGC
    AAGCTGAAGG GTGTGATTTA CTTCCAGGCC ATCGAGGAGG TTTACTACGA TCACCTGCGC
    AGCGCCACAA AGAGCCCGAC CCCGACGCTG ACCTTCTGTG TTAAAACACA CGACAGACTG
    TATTACATGG TGGCTCCGTC CGCAGAGGCC ATGAGGATAT GGATGGATGT GATTGTGACA
    GGAGCCGAGG GCTACACACA GTTCATGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45939
    Clone ID Related Accession (Same CDS sequence) XM_021466364.1
    Accession Version XM_021466364.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGCGTCAGT CAGACTGGGG CAGGCCTCAG ATGCCCGTGC TAGATTTAGA GCACTGGTAC
    CAGGAGGTAA TGGCAGCTGG AGATGTAAAC CAGCTCTGCC CTCCTCCATT GCCGGCTAAA
    GCTTTCTCAT CATCATCATC ATCACCACGC AGGCCTGTGC AGGTGTACAG ATCTCGACTG
    GACAGTGATT GCAGCCAATC CTCACTTAGA CCGAAAGTCA GCGCCGGTTT GTCTCCTACA
    TACACGACGG CCACGCTGGG ACGAAACAGT CCTGCCAGGA GTCCTCTGAT GGTGGCCAAC
    AGCACCGGAA GTCTCCCACG CAACCTGGCA GCCACCCTGC AGGACATCGA GACCAAGAGA
    CAGCTGGCTC TTCAGCAGAA AGGTCAGCAG GTTATCGAGG AGCAGCGCAG GCGTCTGGCG
    GAGCTGAAGC AGAAAGCTGC AGTGGAGGCT CAGTGCCAGT GGGAGGCGCT GCACGGCTCT
    CAAACGCAGC TCCACCAGCC CTTCTCATCA AGCCCAGCAC CGGCGCTTCA CCACTCCATC
    CTGCACCACA CAGCGCCCCC TTGCGGAGAG CAGCCGTACG ACACGCTAAG CCTGGAGAGC
    TCGGACAGCA TGGACACCAG CATCTCTACT GGAAACAACT CGGCCTGCTC GCCGGACAAC
    ATGTCCAGTG CCAGCGGTAT GGATGCGTTG AAGATTGAAG AGATGGAGAA GATGTTGAAG
    GAGGCGCAGC TGGAAAAAGC TCGGCTCATA GAGTCTAGAG AGCGAGAGAC TCAGAGCCGT
    AAACTGATGC TGGAGGAGGA GCGGAGGAGG AGAGAGGAGG CCGAGAAGAG GCTGCAGGAG
    GAGACGCTGC ACAGACAGCA GCTCATCGAG AAGGAGGTCA AGATGAGGGC CAGAAACTTC
    TCTCAGGCCC GTCCGATGAC CCGCTACCTG CCCATCCGCA AAGAGGAGTT TGACCTGCGT
    TCGCACATCG AGTCTTCAGG CCACAGCGTA GACACCTGCT ACCACGTCAT CCTCACCGAG
    AAGATGTGCA AGGGCTTTCT GGTCAAGATG GGAGGCAAGA TCAAATCCTG GAAGAAGCGC
    TGGTTCGTCT TTGATCGCCT CAAAAGGACG TTCTCTTACT ACGCCGACAA ACACGAGAGC
    AAGCTGAAGG GTGTGATTTA CTTCCAGGCC ATCGAGGAGG TTTACTACGA TCACCTGCGC
    AGCGCCACAA AGAAAGGATT TTTCAATCTG AATCTGACAA ACAGCCCGAC CCCGACGCTG
    ACCTTCTGTG TTAAAACACA CGACAGACTG TATTACATGG TGGCTCCGTC CGCAGAGGCC
    ATGAGGATAT GGATGGATGT GATTGTGACA GGAGCCGAGG GCTACACACA GTTCATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322039.1
    CDS473..5275
    Translation

    Target ORF information:

    RefSeq Version XM_021466364.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGCGTCAGT CAGACTGGGG CAGGCCTCAG ATGCCCGTGC TAGATTTAGA GCACTGGTAC
    CAGGAGGTAA TGGCAGCTGG AGATGTAAAC CAGCTCTGCC CTCCTCCATT GCCGGCTAAA
    GCTTTCTCAT CATCATCATC ATCACCACGC AGGCCTGTGC AGGTGTACAG ATCTCGACTG
    GACAGTGATT GCAGCCAATC CTCACTTAGA CCGAAAGTCA GCGCCGGTTT GTCTCCTACA
    TACACGACGG CCACGCTGGG ACGAAACAGT CCTGCCAGGA GTCCTCTGAT GGTGGCCAAC
    AGCACCGGAA GTCTCCCACG CAACCTGGCA GCCACCCTGC AGGACATCGA GACCAAGAGA
    CAGCTGGCTC TTCAGCAGAA AGGTCAGCAG GTTATCGAGG AGCAGCGCAG GCGTCTGGCG
    GAGCTGAAGC AGAAAGCTGC AGTGGAGGCT CAGTGCCAGT GGGAGGCGCT GCACGGCTCT
    CAAACGCAGC TCCACCAGCC CTTCTCATCA AGCCCAGCAC CGGCGCTTCA CCACTCCATC
    CTGCACCACA CAGCGCCCCC TTGCGGAGAG CAGCCGTACG ACACGCTAAG CCTGGAGAGC
    TCGGACAGCA TGGACACCAG CATCTCTACT GGAAACAACT CGGCCTGCTC GCCGGACAAC
    ATGTCCAGTG CCAGCGGTAT GGATGCGTTG AAGATTGAAG AGATGGAGAA GATGTTGAAG
    GAGGCGCAGC TGGAAAAAGC TCGGCTCATA GAGTCTAGAG AGCGAGAGAC TCAGAGCCGT
    AAACTGATGC TGGAGGAGGA GCGGAGGAGG AGAGAGGAGG CCGAGAAGAG GCTGCAGGAG
    GAGACGCTGC ACAGACAGCA GCTCATCGAG AAGGAGGTCA AGATGAGGGC CAGAAACTTC
    TCTCAGGCCC GTCCGATGAC CCGCTACCTG CCCATCCGCA AAGAGGAGTT TGACCTGCGT
    TCGCACATCG AGTCTTCAGG CCACAGCGTA GACACCTGCT ACCACGTCAT CCTCACCGAG
    AAGATGTGCA AGGGCTTTCT GGTCAAGATG GGAGGCAAGA TCAAATCCTG GAAGAAGCGC
    TGGTTCGTCT TTGATCGCCT CAAAAGGACG TTCTCTTACT ACGCCGACAA ACACGAGAGC
    AAGCTGAAGG GTGTGATTTA CTTCCAGGCC ATCGAGGAGG TTTACTACGA TCACCTGCGC
    AGCGCCACAA AGAAAGGATT TTTCAATCTG AATCTGACAA ACAGCCCGAC CCCGACGCTG
    ACCTTCTGTG TTAAAACACA CGACAGACTG TATTACATGG TGGCTCCGTC CGCAGAGGCC
    ATGAGGATAT GGATGGATGT GATTGTGACA GGAGCCGAGG GCTACACACA GTTCATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55240
    Clone ID Related Accession (Same CDS sequence) XM_017351153.1 , XM_017351154.1
    Accession Version XM_017351153.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGGTGTACA GATCTCGACT GGACAGTGAT TGCAGCCAAT CCTCACTTAG ACCGAAAGTC
    AGCGCCGGTT TGTCTCCTAC ATACACGACG GCCACGCTGG GACGAAACAG TCCTGCCAGG
    AGTCCTCTGA TGGTGGCCAA CAGCACCGGA AGTCTCCCAC GCAACCTGGC AGCCACCCTG
    CAGGACATCG AGACCAAGAG ACAGCTGGCT CTTCAGCAGA AAGGTCAGCA GGTTATCGAG
    GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG
    TGGGAGGCGC TGCACGGCTC TCAAACGCAG CTCCACCAGC CCTTCTCATC AAGCCCAGCA
    CCGGCGCTTC ACCACTCCAT CCTGCACCAC ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC
    GACACGCTAA GCCTGGAGAG CTCGGACAGC ATGGACACCA GCATCTCTAC TGGAAACAAC
    TCGGCCTGCT CGCCGGACAA CATGTCCAGT GCCAGCGGTA TGGATGCGTT GAAGATTGAA
    GAGATGGAGA AGATGTTGAA GGAGGCGCAG CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA
    GAGCGAGAGA CTCAGAGCCG TAAACTGATG CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG
    GCCGAGAAGA GGCTGCAGGA GGAGACGCTG CACAGACAGC AGCTCATCGA GAAGGAGGTC
    AAGATGAGGG CCAGAAACTT CTCTCAGGCC CGTCCGATGA CCCGCTACCT GCCCATCCGC
    AAAGAGGAGT TTGACCTGCG TTCGCACATC GAGTCTTCAG GCCACAGCGT AGACACCTGC
    TACCACGTCA TCCTCACCGA GAAGATGTGC AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG
    ATCAAATCCT GGAAGAAGCG CTGGTTCGTC TTTGATCGCC TCAAAAGGAC GTTCTCTTAC
    TACGCCGACA AACACGAGAG CAAGCTGAAG GGTGTGATTT ACTTCCAGGC CATCGAGGAG
    GTTTACTACG ATCACCTGCG CAGCGCCACA AAGAAAGGAT TTTTCAATCT GAATCTGACA
    AACAGCCCGA CCCCGACGCT GACCTTCTGT GTTAAAACAC ACGACAGACT GTATTACATG
    GTGGCTCCGT CCGCAGAGGC CATGAGGATA TGGATGGATG TGATTGTGAC AGGAGCCGAG
    GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206642.1
    CDS460..5103
    Translation

    Target ORF information:

    RefSeq Version XM_017351153.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351153.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGGTGTACA GATCTCGACT GGACAGTGAT TGCAGCCAAT CCTCACTTAG ACCGAAAGTC
    AGCGCCGGTT TGTCTCCTAC ATACACGACG GCCACGCTGG GACGAAACAG TCCTGCCAGG
    AGTCCTCTGA TGGTGGCCAA CAGCACCGGA AGTCTCCCAC GCAACCTGGC AGCCACCCTG
    CAGGACATCG AGACCAAGAG ACAGCTGGCT CTTCAGCAGA AAGGTCAGCA GGTTATCGAG
    GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG
    TGGGAGGCGC TGCACGGCTC TCAAACGCAG CTCCACCAGC CCTTCTCATC AAGCCCAGCA
    CCGGCGCTTC ACCACTCCAT CCTGCACCAC ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC
    GACACGCTAA GCCTGGAGAG CTCGGACAGC ATGGACACCA GCATCTCTAC TGGAAACAAC
    TCGGCCTGCT CGCCGGACAA CATGTCCAGT GCCAGCGGTA TGGATGCGTT GAAGATTGAA
    GAGATGGAGA AGATGTTGAA GGAGGCGCAG CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA
    GAGCGAGAGA CTCAGAGCCG TAAACTGATG CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG
    GCCGAGAAGA GGCTGCAGGA GGAGACGCTG CACAGACAGC AGCTCATCGA GAAGGAGGTC
    AAGATGAGGG CCAGAAACTT CTCTCAGGCC CGTCCGATGA CCCGCTACCT GCCCATCCGC
    AAAGAGGAGT TTGACCTGCG TTCGCACATC GAGTCTTCAG GCCACAGCGT AGACACCTGC
    TACCACGTCA TCCTCACCGA GAAGATGTGC AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG
    ATCAAATCCT GGAAGAAGCG CTGGTTCGTC TTTGATCGCC TCAAAAGGAC GTTCTCTTAC
    TACGCCGACA AACACGAGAG CAAGCTGAAG GGTGTGATTT ACTTCCAGGC CATCGAGGAG
    GTTTACTACG ATCACCTGCG CAGCGCCACA AAGAAAGGAT TTTTCAATCT GAATCTGACA
    AACAGCCCGA CCCCGACGCT GACCTTCTGT GTTAAAACAC ACGACAGACT GTATTACATG
    GTGGCTCCGT CCGCAGAGGC CATGAGGATA TGGATGGATG TGATTGTGAC AGGAGCCGAG
    GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55240
    Clone ID Related Accession (Same CDS sequence) XM_017351153.1 , XM_017351154.1
    Accession Version XM_017351154.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGGTGTACA GATCTCGACT GGACAGTGAT TGCAGCCAAT CCTCACTTAG ACCGAAAGTC
    AGCGCCGGTT TGTCTCCTAC ATACACGACG GCCACGCTGG GACGAAACAG TCCTGCCAGG
    AGTCCTCTGA TGGTGGCCAA CAGCACCGGA AGTCTCCCAC GCAACCTGGC AGCCACCCTG
    CAGGACATCG AGACCAAGAG ACAGCTGGCT CTTCAGCAGA AAGGTCAGCA GGTTATCGAG
    GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG
    TGGGAGGCGC TGCACGGCTC TCAAACGCAG CTCCACCAGC CCTTCTCATC AAGCCCAGCA
    CCGGCGCTTC ACCACTCCAT CCTGCACCAC ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC
    GACACGCTAA GCCTGGAGAG CTCGGACAGC ATGGACACCA GCATCTCTAC TGGAAACAAC
    TCGGCCTGCT CGCCGGACAA CATGTCCAGT GCCAGCGGTA TGGATGCGTT GAAGATTGAA
    GAGATGGAGA AGATGTTGAA GGAGGCGCAG CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA
    GAGCGAGAGA CTCAGAGCCG TAAACTGATG CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG
    GCCGAGAAGA GGCTGCAGGA GGAGACGCTG CACAGACAGC AGCTCATCGA GAAGGAGGTC
    AAGATGAGGG CCAGAAACTT CTCTCAGGCC CGTCCGATGA CCCGCTACCT GCCCATCCGC
    AAAGAGGAGT TTGACCTGCG TTCGCACATC GAGTCTTCAG GCCACAGCGT AGACACCTGC
    TACCACGTCA TCCTCACCGA GAAGATGTGC AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG
    ATCAAATCCT GGAAGAAGCG CTGGTTCGTC TTTGATCGCC TCAAAAGGAC GTTCTCTTAC
    TACGCCGACA AACACGAGAG CAAGCTGAAG GGTGTGATTT ACTTCCAGGC CATCGAGGAG
    GTTTACTACG ATCACCTGCG CAGCGCCACA AAGAAAGGAT TTTTCAATCT GAATCTGACA
    AACAGCCCGA CCCCGACGCT GACCTTCTGT GTTAAAACAC ACGACAGACT GTATTACATG
    GTGGCTCCGT CCGCAGAGGC CATGAGGATA TGGATGGATG TGATTGTGAC AGGAGCCGAG
    GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206643.1
    CDS792..5435
    Translation

    Target ORF information:

    RefSeq Version XM_017351154.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351154.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGGTGTACA GATCTCGACT GGACAGTGAT TGCAGCCAAT CCTCACTTAG ACCGAAAGTC
    AGCGCCGGTT TGTCTCCTAC ATACACGACG GCCACGCTGG GACGAAACAG TCCTGCCAGG
    AGTCCTCTGA TGGTGGCCAA CAGCACCGGA AGTCTCCCAC GCAACCTGGC AGCCACCCTG
    CAGGACATCG AGACCAAGAG ACAGCTGGCT CTTCAGCAGA AAGGTCAGCA GGTTATCGAG
    GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG
    TGGGAGGCGC TGCACGGCTC TCAAACGCAG CTCCACCAGC CCTTCTCATC AAGCCCAGCA
    CCGGCGCTTC ACCACTCCAT CCTGCACCAC ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC
    GACACGCTAA GCCTGGAGAG CTCGGACAGC ATGGACACCA GCATCTCTAC TGGAAACAAC
    TCGGCCTGCT CGCCGGACAA CATGTCCAGT GCCAGCGGTA TGGATGCGTT GAAGATTGAA
    GAGATGGAGA AGATGTTGAA GGAGGCGCAG CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA
    GAGCGAGAGA CTCAGAGCCG TAAACTGATG CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG
    GCCGAGAAGA GGCTGCAGGA GGAGACGCTG CACAGACAGC AGCTCATCGA GAAGGAGGTC
    AAGATGAGGG CCAGAAACTT CTCTCAGGCC CGTCCGATGA CCCGCTACCT GCCCATCCGC
    AAAGAGGAGT TTGACCTGCG TTCGCACATC GAGTCTTCAG GCCACAGCGT AGACACCTGC
    TACCACGTCA TCCTCACCGA GAAGATGTGC AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG
    ATCAAATCCT GGAAGAAGCG CTGGTTCGTC TTTGATCGCC TCAAAAGGAC GTTCTCTTAC
    TACGCCGACA AACACGAGAG CAAGCTGAAG GGTGTGATTT ACTTCCAGGC CATCGAGGAG
    GTTTACTACG ATCACCTGCG CAGCGCCACA AAGAAAGGAT TTTTCAATCT GAATCTGACA
    AACAGCCCGA CCCCGACGCT GACCTTCTGT GTTAAAACAC ACGACAGACT GTATTACATG
    GTGGCTCCGT CCGCAGAGGC CATGAGGATA TGGATGGATG TGATTGTGAC AGGAGCCGAG
    GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55241
    Clone ID Related Accession (Same CDS sequence) XM_017351155.1
    Accession Version XM_017351155.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGGTGTACA GATCTCGACT GGACAGTGAT TGCAGCCAAT CCTCACTTAG ACCGAAAGTC
    AGCGCCGGTT TGTCTCCTAC ATACACGACG GCCACGCTGG GACGAAACAG TCCTGCCAGG
    AGTCCTCTGA TGGTGGCCAA CAGCACCGGA AGTCTCCCAC GCAACCTGGC AGCCACCCTG
    CAGGACATCG AGACCAAGAG ACAGCTGGCT CTTCAGCAGA AAGGTCAGCA GGTTATCGAG
    GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG
    TGGGAGGCGC TGCACGGCTC TCAAACGCAG CTCCACCAGC CCTTCTCATC AAGCCCAGCA
    CCGGCGCTTC ACCACTCCAT CCTGCACCAC ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC
    GACACGCTAA GCCTGGAGAG CTCGGACAGC ATGGACACCA GCATCTCTAC TGGAAACAAC
    TCGGCCTGCT CGCCGGACAA CATGTCCAGT GCCAGCGGTA TGGATGCGTT GAAGATTGAA
    GAGATGGAGA AGATGTTGAA GGAGGCGCAG CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA
    GAGCGAGAGA CTCAGAGCCG TAAACTGATG CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG
    GCCGAGAAGA GGCTGCAGGA GGAGACGCTG CACAGACAGC AGCTCATCGA GAAGGAGGTC
    AAGATGAGGG CCAGAAACTT CTCTCAGGCC CGTCCGATGA CCCGCTACCT GCCCATCCGC
    AAAGAGGAGT TTGACCTGCG TTCGCACATC GAGTCTTCAG GCCACAGCGT AGACACCTGC
    TACCACGTCA TCCTCACCGA GAAGATGTGC AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG
    ATCAAATCCT GGAAGAAGCG CTGGTTCGTC TTTGATCGCC TCAAAAGGAC GTTCTCTTAC
    TACGCCGACA AACACGAGAG CAAGCTGAAG GGTGTGATTT ACTTCCAGGC CATCGAGGAG
    GTTTACTACG ATCACCTGCG CAGCGCCACA AAGAGCCCGA CCCCGACGCT GACCTTCTGT
    GTTAAAACAC ACGACAGACT GTATTACATG GTGGCTCCGT CCGCAGAGGC CATGAGGATA
    TGGATGGATG TGATTGTGAC AGGAGCCGAG GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206644.1
    CDS460..5073
    Translation

    Target ORF information:

    RefSeq Version XM_017351155.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351155.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGA GTACTTGAAG CTGTCGGATG TGTATAAGAT GTATGGGAGT
    GAATGCCATG ACATCCAGCT CGCCAGCGCT TCCTCACACG GCCTCTCGCT TGCCCTTGAC
    CCAGCTGAAA CTGCCCCGCG TGAGGAGTAT GTGACGGTCA GCCAATTAAA CCAGATGTAC
    GGGATGCCAA AGGTGGAGTC TTCCCCTACC TCACCCGTCC AATCAGTGCA GCCCTCTCTC
    GCAGACTCCA CCTTCCCCCG CCTCCCTTCC CCTCATGGCT CCTCCCTCTC TCTCTCCTTC
    GAGGTGTACA GATCTCGACT GGACAGTGAT TGCAGCCAAT CCTCACTTAG ACCGAAAGTC
    AGCGCCGGTT TGTCTCCTAC ATACACGACG GCCACGCTGG GACGAAACAG TCCTGCCAGG
    AGTCCTCTGA TGGTGGCCAA CAGCACCGGA AGTCTCCCAC GCAACCTGGC AGCCACCCTG
    CAGGACATCG AGACCAAGAG ACAGCTGGCT CTTCAGCAGA AAGGTCAGCA GGTTATCGAG
    GAGCAGCGCA GGCGTCTGGC GGAGCTGAAG CAGAAAGCTG CAGTGGAGGC TCAGTGCCAG
    TGGGAGGCGC TGCACGGCTC TCAAACGCAG CTCCACCAGC CCTTCTCATC AAGCCCAGCA
    CCGGCGCTTC ACCACTCCAT CCTGCACCAC ACAGCGCCCC CTTGCGGAGA GCAGCCGTAC
    GACACGCTAA GCCTGGAGAG CTCGGACAGC ATGGACACCA GCATCTCTAC TGGAAACAAC
    TCGGCCTGCT CGCCGGACAA CATGTCCAGT GCCAGCGGTA TGGATGCGTT GAAGATTGAA
    GAGATGGAGA AGATGTTGAA GGAGGCGCAG CTGGAAAAAG CTCGGCTCAT AGAGTCTAGA
    GAGCGAGAGA CTCAGAGCCG TAAACTGATG CTGGAGGAGG AGCGGAGGAG GAGAGAGGAG
    GCCGAGAAGA GGCTGCAGGA GGAGACGCTG CACAGACAGC AGCTCATCGA GAAGGAGGTC
    AAGATGAGGG CCAGAAACTT CTCTCAGGCC CGTCCGATGA CCCGCTACCT GCCCATCCGC
    AAAGAGGAGT TTGACCTGCG TTCGCACATC GAGTCTTCAG GCCACAGCGT AGACACCTGC
    TACCACGTCA TCCTCACCGA GAAGATGTGC AAGGGCTTTC TGGTCAAGAT GGGAGGCAAG
    ATCAAATCCT GGAAGAAGCG CTGGTTCGTC TTTGATCGCC TCAAAAGGAC GTTCTCTTAC
    TACGCCGACA AACACGAGAG CAAGCTGAAG GGTGTGATTT ACTTCCAGGC CATCGAGGAG
    GTTTACTACG ATCACCTGCG CAGCGCCACA AAGAGCCCGA CCCCGACGCT GACCTTCTGT
    GTTAAAACAC ACGACAGACT GTATTACATG GTGGCTCCGT CCGCAGAGGC CATGAGGATA
    TGGATGGATG TGATTGTGAC AGGAGCCGAG GGCTACACAC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55242
    Clone ID Related Accession (Same CDS sequence) XM_017351156.1
    Accession Version XM_017351156.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4608bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGCTCA AGAAGGTCCG ATTTCAGGAT TCTCATGTCC CCGAGCAAGT AAGTGACAGT 
    ACACTGGCCA TGGATACTTT GAACCAAAAT CTTAGCCATG GGCATCAGTC TCATCAGGTG
    CTCCAGAGTA CTCCACTGGA TCTTATTGAG ACTGGAAAGG CACTGAAGGT TCAAGCAGAG
    CGCCCCCATT TAGTCAGTCT GGGAAGTGGG CGTCTCAGTA CAGCCATCAC CCTCTTACCC
    CTGCCTGAAG GGAAGACGAC TGTGGGTCAT GGAGCCATGG ACATCAGCAT ACAGGGCCCC
    GGGGTCGCAG CTCAGCACTG CTTTATTGAG AACAGGGCTG GAATCATCAC ACTTCACCCC
    TGTGGAAACC AGTGCAGCAT GGACGGACTG CCGGTCAACA AACCTGTGCG ACTCTCTCAA
    GGCTGCATGC TGTGTTTCGG CCAATCCGCC TTCTTCCGCT TCAACCACCC CGAGGAGGCT
    TTCCGAATGA AAAGCATGAT GCCCGAGGGA GGACAGACAG CCAGTGTGAA AAACTGGACC
    CACAAAGATT CAGAGAACCT GGTCAATGGG AACCACTCTT CAGGAGAGGC AAAATCCCAC
    TTGGTTTCCA ACGAGAGAGT GCGCCCTGAG CACAGTGCCA TCGTCAGTTC CATTGAGAAG
    GACCTTCAGG ACATTATGGA CTCATTAGTC ATGGATGACC CTCAGCCCTC ATCCTCCGAG
    TCAAAGAAGC CCTCTAGTCA GTCCATCTCA CAGTCTCCCC TTTCTCCGAT GTTAAATGGA
    GGGGGGCGCT TCCTTCTCTC TCCCCCAACG AGCCCTGGTG CTATGTCTGT GGGCTCCAGT
    TATGAGAATA ACTCCCCTCC TTTCTCTCCT CTGTCATCTC CCTCGGCTGC CAGCAGTGGC
    AGCTACACCA CTCTCTCACC AATTGGTTGT CAGGACCAGC CTCACATGCT TCCTCCTGTG
    CCAGTGAGGT CTTCTAGTTA CAATTATACC AGCCAGCCAC CCATACCTCA GCCACGGACC
    ATACTGCCCA GCTATTCCAG TGGCAGCAGT GGGCCAAAGG TGCCTGAGAG TCCCAGACTC
    CAGCGAAAGG CGCTTTTAGA AGCACCGCCA AGCCCAAAGC CAAGCCGTAG GGGGTTGAAC
    CAAGACAGCT CGGTGGCTAA AACCCCAGAC AGCCCCATAC AGACTCACAT TCTGCCCTCA
    GTCTCCATCT CTACAGCTCC GACTGATGTC CCATCTGTTA GTCGGGTACC AGTTCCAGGT
    AGTCCTAGAT TAGCCACCAA ATTCTCCACT GCGTCCTCAC CTGCCTCACC CTCTAGTCCC
    AGAACCAAAA CTACCATTGT CCTCCAAGAT CGACCCTCAA GTCCTTTCCG GGAGCAACCT
    CAGCCAGATC GTTCTTTGAC GTCCAGCCCC TCTCACCAAC TCTCCCCACC ATCAAGATCT
    TTCCAGCCCC CATTGGACCC CATTGTTCAC ATCATTCAAG GTGGACCTTC ACACCCACAT
    CCTCGAACAC TGCAGCCTCC AGAAAGTCCG CGGCTGGCCC GCAGAAATCT TGAAGGGAGC
    AGCATGAGGG AACTCCCCCC TCTCAGTCCC TCCATAGCTC GCAGGGGGGT TCCTGTGCTC
    CCAGGTGCTC TACCAGGAAC CCTGCGAACT CCAGAGAGCC CATCCCCAAG AATGGTGCCA
    GAGAGTCCCA GACTCAGGAG GAAAACAGGG TCACCTACAG AGGAGCCATT CAGTCCTCGT
    GCGATGCGCA CTCGTAGTCC TTCGCCCACC TCTGGACTGA CTGGCGAAAG CAGTGGACGG
    AAGGGAAGTT TTGGGAACTC ACTCTCTTCT GCCTTCAGCC TAAGTTCTCT GCCTGGGTCT
    TCCCCACGAT CTAGTCCCAG GAGCCACCGA AAGATGTCAG CAGGCCACAG AGACCTCCGG
    ATGCCTCACC CAGGCATGAG GGAGCGCAAA AACAGCATCA CAGAGATCAG CGACAACGAG
    GATGACCTTC TGGAGTACCA TCGACGCCAA AGAGAAGAGC GACTCAGAGA GCAGGAAATG
    GAGAGACTGG AACGGCAGCG GCTGGAGACA ATCTTGAACC TCTGCGCTGA GTATAACAAG
    GGCGACGGGC CGCCGGGTGA TTTGGGCAGG ATGGGCGTCT CTGGGCTTGG CTCGAGGGAC
    GGGCTTAACG TCAGGCGTCC TTCAATGGAC AGCGTGAACA GCGCTTCACT AAGAGCGACT
    CAAAGACAAC GGGAAAGCCA AGAGGACAAC CTGAAGGAGG ATAGCAGTAG CACTGAGAGT
    GCGCAGCTGG ACGGACGGAC ACCTGCTCTG CTAAATGGAC AGAATGGACA GGCTGAGGAG
    TCTCTGGGTT CGGGCAGCAT TGGCCGTCTG GAGCTGGGCT ACCTGGAGGA GGAGCGGGTT
    CGAGTGCTGG CAAAGGTAGA TGAGCTCAAA GCTCGAATCA CAGAACTGGA GCAGCAACTT
    CAGGAGTCTA AACAAGAGGC GGAGATGGAG AGAGCTCTGC TGCAGGGAGA AAGACAGGCT
    GAGATGGAGC AAATCGAGAC TGAGACCGAG ATTATCAACC AGCTGCAGCG CAAACTCACT
    GAACTGGAGA GCAGCATCCA GAGAGAGAAG GACAAGGAGA GGGCTAATGT TGACGCGGAG
    CGGGAAGCCC TGGAGAGGCT TCAGGATGGG TACAATGAGT TGACGAGCCA GCTACATAAC
    TGCCCTGAGT CTCTGAGGGA ACAGTTACAG GAACAGCTCA AGAGGGAAGC TGAGACTCTT
    GAAGCAGCGA CAAAGCAGTT TGAAGACCTG GAGTTTCAGC AGTTGGAGAG GGAGAGCAGT
    CTGGAGGAGG AGCGCGAGAC CATCAGCCAG CAGCTCATGC AGGACAGAGC TCAATACCAC
    AGCAGTGTCG CCAAGAGAAA GGAAAAGGTG GTGGCATTGG AGAGCCAGGC GAGCCAGCTG
    GGAATACAGG CCGCTCAGGA GTGTGAGAGA CTGGCCAAGG ACAGAACCAT CACCTTGCAG
    ATGCTACACA AGGAAAAGGA AAGGCTGGCC AGTCTGGAGA GAAGATACCA GAGTTTAACT
    GGTGGAAAGA CGTTCCCCAA AAGCTCCAAT ACTATAAAGG AGGAGTACTT GAAGCTGTCG
    GATGTGTATA AGATGTATGG GAGTGAATGC CATGACATCC AGCTCGCCAG CGCTTCCTCA
    CACGGCCTCT CGCTTGCCCT TGACCCAGCT GAAACTGCCC CGCGTGAGGA GTATGTGACG
    GTCAGCCAAT TAAACCAGAT GTACGGGATG CCAAAGGTGG AGTCTTCCCC TACCTCACCC
    GTCCAATCAG TGCAGCCCTC TCTCGCAGAC TCCACCTTCC CCCGCCTCCC TTCCCCTCAT
    GGCTCCTCCC TCTCTCTCTC CTTCGAGGTG TACAGATCTC GACTGGACAG TGATTGCAGC
    CAATCCTCAC TTAGACCGAA AGTCAGCGCC GGTTTGTCTC CTACATACAC GACGGCCACG
    CTGGGACGAA ACAGTCCTGC CAGGAGTCCT CTGATGGTGG CCAACAGCAC CGGAAGTCTC
    CCACGCAACC TGGCAGCCAC CCTGCAGGAC ATCGAGACCA AGAGACAGCT GGCTCTTCAG
    CAGAAAGGTC AGCAGGTTAT CGAGGAGCAG CGCAGGCGTC TGGCGGAGCT GAAGCAGAAA
    GCTGCAGTGG AGGCTCAGTG CCAGTGGGAG GCGCTGCACG GCTCTCAAAC GCAGCTCCAC
    CAGCCCTTCT CATCAAGCCC AGCACCGGCG CTTCACCACT CCATCCTGCA CCACACAGCG
    CCCCCTTGCG GAGAGCAGCC GTACGACACG CTAAGCCTGG AGAGCTCGGA CAGCATGGAC
    ACCAGCATCT CTACTGGAAA CAACTCGGCC TGCTCGCCGG ACAACATGTC CAGTGCCAGC
    GGTATGGATG CGTTGAAGAT TGAAGAGATG GAGAAGATGT TGAAGGAGGC GCAGCTGGAA
    AAAGCTCGGC TCATAGAGTC TAGAGAGCGA GAGACTCAGA GCCGTAAACT GATGCTGGAG
    GAGGAGCGGA GGAGGAGAGA GGAGGCCGAG AAGAGGCTGC AGGAGGAGAC GCTGCACAGA
    CAGCAGCTCA TCGAGAAGGA GGTCAAGATG AGGGCCAGAA ACTTCTCTCA GGCCCGTCCG
    ATGACCCGCT ACCTGCCCAT CCGCAAAGAG GAGTTTGACC TGCGTTCGCA CATCGAGTCT
    TCAGGCCACA GCGTAGACAC CTGCTACCAC GTCATCCTCA CCGAGAAGAT GTGCAAGGGC
    TTTCTGGTCA AGATGGGAGG CAAGATCAAA TCCTGGAAGA AGCGCTGGTT CGTCTTTGAT
    CGCCTCAAAA GGACGTTCTC TTACTACGCC GACAAACACG AGAGCAAGCT GAAGGGTGTG
    ATTTACTTCC AGGCCATCGA GGAGGTTTAC TACGATCACC TGCGCAGCGC CACAAAGAAA
    GGATTTTTCA ATCTGAATCT GACAAACAGC CCGACCCCGA CGCTGACCTT CTGTGTTAAA
    ACACACGACA GACTGTATTA CATGGTGGCT CCGTCCGCAG AGGCCATGAG GATATGGATG
    GATGTGATTG TGACAGGAGC CGAGGGCTAC ACACAGTTCA TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206645.1
    CDS235..4842
    Translation

    Target ORF information:

    RefSeq Version XM_017351156.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGCTCA AGAAGGTCCG ATTTCAGGAT TCTCATGTCC CCGAGCAAGT AAGTGACAGT 
    ACACTGGCCA TGGATACTTT GAACCAAAAT CTTAGCCATG GGCATCAGTC TCATCAGGTG
    CTCCAGAGTA CTCCACTGGA TCTTATTGAG ACTGGAAAGG CACTGAAGGT TCAAGCAGAG
    CGCCCCCATT TAGTCAGTCT GGGAAGTGGG CGTCTCAGTA CAGCCATCAC CCTCTTACCC
    CTGCCTGAAG GGAAGACGAC TGTGGGTCAT GGAGCCATGG ACATCAGCAT ACAGGGCCCC
    GGGGTCGCAG CTCAGCACTG CTTTATTGAG AACAGGGCTG GAATCATCAC ACTTCACCCC
    TGTGGAAACC AGTGCAGCAT GGACGGACTG CCGGTCAACA AACCTGTGCG ACTCTCTCAA
    GGCTGCATGC TGTGTTTCGG CCAATCCGCC TTCTTCCGCT TCAACCACCC CGAGGAGGCT
    TTCCGAATGA AAAGCATGAT GCCCGAGGGA GGACAGACAG CCAGTGTGAA AAACTGGACC
    CACAAAGATT CAGAGAACCT GGTCAATGGG AACCACTCTT CAGGAGAGGC AAAATCCCAC
    TTGGTTTCCA ACGAGAGAGT GCGCCCTGAG CACAGTGCCA TCGTCAGTTC CATTGAGAAG
    GACCTTCAGG ACATTATGGA CTCATTAGTC ATGGATGACC CTCAGCCCTC ATCCTCCGAG
    TCAAAGAAGC CCTCTAGTCA GTCCATCTCA CAGTCTCCCC TTTCTCCGAT GTTAAATGGA
    GGGGGGCGCT TCCTTCTCTC TCCCCCAACG AGCCCTGGTG CTATGTCTGT GGGCTCCAGT
    TATGAGAATA ACTCCCCTCC TTTCTCTCCT CTGTCATCTC CCTCGGCTGC CAGCAGTGGC
    AGCTACACCA CTCTCTCACC AATTGGTTGT CAGGACCAGC CTCACATGCT TCCTCCTGTG
    CCAGTGAGGT CTTCTAGTTA CAATTATACC AGCCAGCCAC CCATACCTCA GCCACGGACC
    ATACTGCCCA GCTATTCCAG TGGCAGCAGT GGGCCAAAGG TGCCTGAGAG TCCCAGACTC
    CAGCGAAAGG CGCTTTTAGA AGCACCGCCA AGCCCAAAGC CAAGCCGTAG GGGGTTGAAC
    CAAGACAGCT CGGTGGCTAA AACCCCAGAC AGCCCCATAC AGACTCACAT TCTGCCCTCA
    GTCTCCATCT CTACAGCTCC GACTGATGTC CCATCTGTTA GTCGGGTACC AGTTCCAGGT
    AGTCCTAGAT TAGCCACCAA ATTCTCCACT GCGTCCTCAC CTGCCTCACC CTCTAGTCCC
    AGAACCAAAA CTACCATTGT CCTCCAAGAT CGACCCTCAA GTCCTTTCCG GGAGCAACCT
    CAGCCAGATC GTTCTTTGAC GTCCAGCCCC TCTCACCAAC TCTCCCCACC ATCAAGATCT
    TTCCAGCCCC CATTGGACCC CATTGTTCAC ATCATTCAAG GTGGACCTTC ACACCCACAT
    CCTCGAACAC TGCAGCCTCC AGAAAGTCCG CGGCTGGCCC GCAGAAATCT TGAAGGGAGC
    AGCATGAGGG AACTCCCCCC TCTCAGTCCC TCCATAGCTC GCAGGGGGGT TCCTGTGCTC
    CCAGGTGCTC TACCAGGAAC CCTGCGAACT CCAGAGAGCC CATCCCCAAG AATGGTGCCA
    GAGAGTCCCA GACTCAGGAG GAAAACAGGG TCACCTACAG AGGAGCCATT CAGTCCTCGT
    GCGATGCGCA CTCGTAGTCC TTCGCCCACC TCTGGACTGA CTGGCGAAAG CAGTGGACGG
    AAGGGAAGTT TTGGGAACTC ACTCTCTTCT GCCTTCAGCC TAAGTTCTCT GCCTGGGTCT
    TCCCCACGAT CTAGTCCCAG GAGCCACCGA AAGATGTCAG CAGGCCACAG AGACCTCCGG
    ATGCCTCACC CAGGCATGAG GGAGCGCAAA AACAGCATCA CAGAGATCAG CGACAACGAG
    GATGACCTTC TGGAGTACCA TCGACGCCAA AGAGAAGAGC GACTCAGAGA GCAGGAAATG
    GAGAGACTGG AACGGCAGCG GCTGGAGACA ATCTTGAACC TCTGCGCTGA GTATAACAAG
    GGCGACGGGC CGCCGGGTGA TTTGGGCAGG ATGGGCGTCT CTGGGCTTGG CTCGAGGGAC
    GGGCTTAACG TCAGGCGTCC TTCAATGGAC AGCGTGAACA GCGCTTCACT AAGAGCGACT
    CAAAGACAAC GGGAAAGCCA AGAGGACAAC CTGAAGGAGG ATAGCAGTAG CACTGAGAGT
    GCGCAGCTGG ACGGACGGAC ACCTGCTCTG CTAAATGGAC AGAATGGACA GGCTGAGGAG
    TCTCTGGGTT CGGGCAGCAT TGGCCGTCTG GAGCTGGGCT ACCTGGAGGA GGAGCGGGTT
    CGAGTGCTGG CAAAGGTAGA TGAGCTCAAA GCTCGAATCA CAGAACTGGA GCAGCAACTT
    CAGGAGTCTA AACAAGAGGC GGAGATGGAG AGAGCTCTGC TGCAGGGAGA AAGACAGGCT
    GAGATGGAGC AAATCGAGAC TGAGACCGAG ATTATCAACC AGCTGCAGCG CAAACTCACT
    GAACTGGAGA GCAGCATCCA GAGAGAGAAG GACAAGGAGA GGGCTAATGT TGACGCGGAG
    CGGGAAGCCC TGGAGAGGCT TCAGGATGGG TACAATGAGT TGACGAGCCA GCTACATAAC
    TGCCCTGAGT CTCTGAGGGA ACAGTTACAG GAACAGCTCA AGAGGGAAGC TGAGACTCTT
    GAAGCAGCGA CAAAGCAGTT TGAAGACCTG GAGTTTCAGC AGTTGGAGAG GGAGAGCAGT
    CTGGAGGAGG AGCGCGAGAC CATCAGCCAG CAGCTCATGC AGGACAGAGC TCAATACCAC
    AGCAGTGTCG CCAAGAGAAA GGAAAAGGTG GTGGCATTGG AGAGCCAGGC GAGCCAGCTG
    GGAATACAGG CCGCTCAGGA GTGTGAGAGA CTGGCCAAGG ACAGAACCAT CACCTTGCAG
    ATGCTACACA AGGAAAAGGA AAGGCTGGCC AGTCTGGAGA GAAGATACCA GAGTTTAACT
    GGTGGAAAGA CGTTCCCCAA AAGCTCCAAT ACTATAAAGG AGGAGTACTT GAAGCTGTCG
    GATGTGTATA AGATGTATGG GAGTGAATGC CATGACATCC AGCTCGCCAG CGCTTCCTCA
    CACGGCCTCT CGCTTGCCCT TGACCCAGCT GAAACTGCCC CGCGTGAGGA GTATGTGACG
    GTCAGCCAAT TAAACCAGAT GTACGGGATG CCAAAGGTGG AGTCTTCCCC TACCTCACCC
    GTCCAATCAG TGCAGCCCTC TCTCGCAGAC TCCACCTTCC CCCGCCTCCC TTCCCCTCAT
    GGCTCCTCCC TCTCTCTCTC CTTCGAGGTG TACAGATCTC GACTGGACAG TGATTGCAGC
    CAATCCTCAC TTAGACCGAA AGTCAGCGCC GGTTTGTCTC CTACATACAC GACGGCCACG
    CTGGGACGAA ACAGTCCTGC CAGGAGTCCT CTGATGGTGG CCAACAGCAC CGGAAGTCTC
    CCACGCAACC TGGCAGCCAC CCTGCAGGAC ATCGAGACCA AGAGACAGCT GGCTCTTCAG
    CAGAAAGGTC AGCAGGTTAT CGAGGAGCAG CGCAGGCGTC TGGCGGAGCT GAAGCAGAAA
    GCTGCAGTGG AGGCTCAGTG CCAGTGGGAG GCGCTGCACG GCTCTCAAAC GCAGCTCCAC
    CAGCCCTTCT CATCAAGCCC AGCACCGGCG CTTCACCACT CCATCCTGCA CCACACAGCG
    CCCCCTTGCG GAGAGCAGCC GTACGACACG CTAAGCCTGG AGAGCTCGGA CAGCATGGAC
    ACCAGCATCT CTACTGGAAA CAACTCGGCC TGCTCGCCGG ACAACATGTC CAGTGCCAGC
    GGTATGGATG CGTTGAAGAT TGAAGAGATG GAGAAGATGT TGAAGGAGGC GCAGCTGGAA
    AAAGCTCGGC TCATAGAGTC TAGAGAGCGA GAGACTCAGA GCCGTAAACT GATGCTGGAG
    GAGGAGCGGA GGAGGAGAGA GGAGGCCGAG AAGAGGCTGC AGGAGGAGAC GCTGCACAGA
    CAGCAGCTCA TCGAGAAGGA GGTCAAGATG AGGGCCAGAA ACTTCTCTCA GGCCCGTCCG
    ATGACCCGCT ACCTGCCCAT CCGCAAAGAG GAGTTTGACC TGCGTTCGCA CATCGAGTCT
    TCAGGCCACA GCGTAGACAC CTGCTACCAC GTCATCCTCA CCGAGAAGAT GTGCAAGGGC
    TTTCTGGTCA AGATGGGAGG CAAGATCAAA TCCTGGAAGA AGCGCTGGTT CGTCTTTGAT
    CGCCTCAAAA GGACGTTCTC TTACTACGCC GACAAACACG AGAGCAAGCT GAAGGGTGTG
    ATTTACTTCC AGGCCATCGA GGAGGTTTAC TACGATCACC TGCGCAGCGC CACAAAGAAA
    GGATTTTTCA ATCTGAATCT GACAAACAGC CCGACCCCGA CGCTGACCTT CTGTGTTAAA
    ACACACGACA GACTGTATTA CATGGTGGCT CCGTCCGCAG AGGCCATGAG GATATGGATG
    GATGTGATTG TGACAGGAGC CGAGGGCTAC ACACAGTTCA TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55243
    Clone ID Related Accession (Same CDS sequence) XM_017351157.1
    Accession Version XM_017351157.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4515bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAAGCTGA GACTCTTGAA GCAGCGACAA AGCAGTTTGA AGACCTGGAG
    TTTCAGCAGT TGGAGAGGGA GAGCAGTCTG GAGGAGGAGC GCGAGACCAT CAGCCAGCAG
    CTCATGCAGG ACAGAGCTCA ATACCACAGC AGTGTCGCCA AGAGAAAGGA AAAGGTGGTG
    GCATTGGAGA GCCAGGCGAG CCAGCTGGGA ATACAGGCCG CTCAGGAGTG TGAGAGACTG
    GCCAAGGACA GAACCATCAC CTTGCAGATG CTACACAAGG AAAAGGAAAG GCTGGCCAGT
    CTGGAGAGAA GATACCAGAG TTTAACTGGT GGAAAGACGT TCCCCAAAAG CTCCAATACT
    ATAAAGGAGG AGTACTTGAA GCTGTCGGAT GTGTATAAGA TGTATGGGAG TGAATGCCAT
    GACATCCAGC TCGCCAGCGC TTCCTCACAC GGCCTCTCGC TTGCCCTTGA CCCAGCTGAA
    ACTGCCCCGC GTGAGGAGTA TGTGACGGTC AGCCAATTAA ACCAGATGTA CGGGATGCCA
    AAGGTGGAGT CTTCCCCTAC CTCACCCGTC CAATCAGTGC AGCCCTCTCT CGCAGACTCC
    ACCTTCCCCC GCCTCCCTTC CCCTCATGGC TCCTCCCTCT CTCTCTCCTT CGAGGTGTAC
    AGATCTCGAC TGGACAGTGA TTGCAGCCAA TCCTCACTTA GACCGAAAGT CAGCGCCGGT
    TTGTCTCCTA CATACACGAC GGCCACGCTG GGACGAAACA GTCCTGCCAG GAGTCCTCTG
    ATGGTGGCCA ACAGCACCGG AAGTCTCCCA CGCAACCTGG CAGCCACCCT GCAGGACATC
    GAGACCAAGA GACAGCTGGC TCTTCAGCAG AAAGGTCAGC AGGTTATCGA GGAGCAGCGC
    AGGCGTCTGG CGGAGCTGAA GCAGAAAGCT GCAGTGGAGG CTCAGTGCCA GTGGGAGGCG
    CTGCACGGCT CTCAAACGCA GCTCCACCAG CCCTTCTCAT CAAGCCCAGC ACCGGCGCTT
    CACCACTCCA TCCTGCACCA CACAGCGCCC CCTTGCGGAG AGCAGCCGTA CGACACGCTA
    AGCCTGGAGA GCTCGGACAG CATGGACACC AGCATCTCTA CTGGAAACAA CTCGGCCTGC
    TCGCCGGACA ACATGTCCAG TGCCAGCGGT ATGGATGCGT TGAAGATTGA AGAGATGGAG
    AAGATGTTGA AGGAGGCGCA GCTGGAAAAA GCTCGGCTCA TAGAGTCTAG AGAGCGAGAG
    ACTCAGAGCC GTAAACTGAT GCTGGAGGAG GAGCGGAGGA GGAGAGAGGA GGCCGAGAAG
    AGGCTGCAGG AGGAGACGCT GCACAGACAG CAGCTCATCG AGAAGGAGGT CAAGATGAGG
    GCCAGAAACT TCTCTCAGGC CCGTCCGATG ACCCGCTACC TGCCCATCCG CAAAGAGGAG
    TTTGACCTGC GTTCGCACAT CGAGTCTTCA GGCCACAGCG TAGACACCTG CTACCACGTC
    ATCCTCACCG AGAAGATGTG CAAGGGCTTT CTGGTCAAGA TGGGAGGCAA GATCAAATCC
    TGGAAGAAGC GCTGGTTCGT CTTTGATCGC CTCAAAAGGA CGTTCTCTTA CTACGCCGAC
    AAACACGAGA GCAAGCTGAA GGGTGTGATT TACTTCCAGG CCATCGAGGA GGTTTACTAC
    GATCACCTGC GCAGCGCCAC AAAGAAAGGA TTTTTCAATC TGAATCTGAC AAACAGCCCG
    ACCCCGACGC TGACCTTCTG TGTTAAAACA CACGACAGAC TGTATTACAT GGTGGCTCCG
    TCCGCAGAGG CCATGAGGAT ATGGATGGAT GTGATTGTGA CAGGAGCCGA GGGCTACACA
    CAGTTCATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206646.1
    CDS460..4974
    Translation

    Target ORF information:

    RefSeq Version XM_017351157.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351157.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAAGCTGA GACTCTTGAA GCAGCGACAA AGCAGTTTGA AGACCTGGAG
    TTTCAGCAGT TGGAGAGGGA GAGCAGTCTG GAGGAGGAGC GCGAGACCAT CAGCCAGCAG
    CTCATGCAGG ACAGAGCTCA ATACCACAGC AGTGTCGCCA AGAGAAAGGA AAAGGTGGTG
    GCATTGGAGA GCCAGGCGAG CCAGCTGGGA ATACAGGCCG CTCAGGAGTG TGAGAGACTG
    GCCAAGGACA GAACCATCAC CTTGCAGATG CTACACAAGG AAAAGGAAAG GCTGGCCAGT
    CTGGAGAGAA GATACCAGAG TTTAACTGGT GGAAAGACGT TCCCCAAAAG CTCCAATACT
    ATAAAGGAGG AGTACTTGAA GCTGTCGGAT GTGTATAAGA TGTATGGGAG TGAATGCCAT
    GACATCCAGC TCGCCAGCGC TTCCTCACAC GGCCTCTCGC TTGCCCTTGA CCCAGCTGAA
    ACTGCCCCGC GTGAGGAGTA TGTGACGGTC AGCCAATTAA ACCAGATGTA CGGGATGCCA
    AAGGTGGAGT CTTCCCCTAC CTCACCCGTC CAATCAGTGC AGCCCTCTCT CGCAGACTCC
    ACCTTCCCCC GCCTCCCTTC CCCTCATGGC TCCTCCCTCT CTCTCTCCTT CGAGGTGTAC
    AGATCTCGAC TGGACAGTGA TTGCAGCCAA TCCTCACTTA GACCGAAAGT CAGCGCCGGT
    TTGTCTCCTA CATACACGAC GGCCACGCTG GGACGAAACA GTCCTGCCAG GAGTCCTCTG
    ATGGTGGCCA ACAGCACCGG AAGTCTCCCA CGCAACCTGG CAGCCACCCT GCAGGACATC
    GAGACCAAGA GACAGCTGGC TCTTCAGCAG AAAGGTCAGC AGGTTATCGA GGAGCAGCGC
    AGGCGTCTGG CGGAGCTGAA GCAGAAAGCT GCAGTGGAGG CTCAGTGCCA GTGGGAGGCG
    CTGCACGGCT CTCAAACGCA GCTCCACCAG CCCTTCTCAT CAAGCCCAGC ACCGGCGCTT
    CACCACTCCA TCCTGCACCA CACAGCGCCC CCTTGCGGAG AGCAGCCGTA CGACACGCTA
    AGCCTGGAGA GCTCGGACAG CATGGACACC AGCATCTCTA CTGGAAACAA CTCGGCCTGC
    TCGCCGGACA ACATGTCCAG TGCCAGCGGT ATGGATGCGT TGAAGATTGA AGAGATGGAG
    AAGATGTTGA AGGAGGCGCA GCTGGAAAAA GCTCGGCTCA TAGAGTCTAG AGAGCGAGAG
    ACTCAGAGCC GTAAACTGAT GCTGGAGGAG GAGCGGAGGA GGAGAGAGGA GGCCGAGAAG
    AGGCTGCAGG AGGAGACGCT GCACAGACAG CAGCTCATCG AGAAGGAGGT CAAGATGAGG
    GCCAGAAACT TCTCTCAGGC CCGTCCGATG ACCCGCTACC TGCCCATCCG CAAAGAGGAG
    TTTGACCTGC GTTCGCACAT CGAGTCTTCA GGCCACAGCG TAGACACCTG CTACCACGTC
    ATCCTCACCG AGAAGATGTG CAAGGGCTTT CTGGTCAAGA TGGGAGGCAA GATCAAATCC
    TGGAAGAAGC GCTGGTTCGT CTTTGATCGC CTCAAAAGGA CGTTCTCTTA CTACGCCGAC
    AAACACGAGA GCAAGCTGAA GGGTGTGATT TACTTCCAGG CCATCGAGGA GGTTTACTAC
    GATCACCTGC GCAGCGCCAC AAAGAAAGGA TTTTTCAATC TGAATCTGAC AAACAGCCCG
    ACCCCGACGC TGACCTTCTG TGTTAAAACA CACGACAGAC TGTATTACAT GGTGGCTCCG
    TCCGCAGAGG CCATGAGGAT ATGGATGGAT GTGATTGTGA CAGGAGCCGA GGGCTACACA
    CAGTTCATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45942
    Clone ID Related Accession (Same CDS sequence) XM_005157452.3 , XM_005157452.4
    Accession Version XM_005157452.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157452.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGT GTACAGATCT CGACTGGACA GTGATTGCAG CCAATCCTCA
    CTTAGACCGA AAGTCAGCGC CGGTTTGTCT CCTACATACA CGACGGCCAC GCTGGGACGA
    AACAGTCCTG CCAGGAGTCC TCTGATGGTG GCCAACAGCA CCGGAAGTCT CCCACGCAAC
    CTGGCAGCCA CCCTGCAGGA CATCGAGACC AAGAGACAGC TGGCTCTTCA GCAGAAAGGT
    CAGCAGGTTA TCGAGGAGCA GCGCAGGCGT CTGGCGGAGC TGAAGCAGAA AGCTGCAGTG
    GAGGCTCAGT GCCAGTGGGA GGCGCTGCAC GGCTCTCAAA CGCAGCTCCA CCAGCCCTTC
    TCATCAAGCC CAGCACCGGC GCTTCACCAC TCCATCCTGC ACCACACAGC GCCCCCTTGC
    GGAGAGCAGC CGTACGACAC GCTAAGCCTG GAGAGCTCGG ACAGCATGGA CACCAGCATC
    TCTACTGGAA ACAACTCGGC CTGCTCGCCG GACAACATGT CCAGTGCCAG CGGTATGGAT
    GCGTTGAAGA TTGAAGAGAT GGAGAAGATG TTGAAGGAGG CGCAGCTGGA AAAAGCTCGG
    CTCATAGAGT CTAGAGAGCG AGAGACTCAG AGCCGTAAAC TGATGCTGGA GGAGGAGCGG
    AGGAGGAGAG AGGAGGCCGA GAAGAGGCTG CAGGAGGAGA CGCTGCACAG ACAGCAGCTC
    ATCGAGAAGG AGGTCAAGAT GAGGGCCAGA AACTTCTCTC AGGCCCGTCC GATGACCCGC
    TACCTGCCCA TCCGCAAAGA GGAGTTTGAC CTGCGTTCGC ACATCGAGTC TTCAGGCCAC
    AGCGTAGACA CCTGCTACCA CGTCATCCTC ACCGAGAAGA TGTGCAAGGG CTTTCTGGTC
    AAGATGGGAG GCAAGATCAA ATCCTGGAAG AAGCGCTGGT TCGTCTTTGA TCGCCTCAAA
    AGGACGTTCT CTTACTACGC CGACAAACAC GAGAGCAAGC TGAAGGGTGT GATTTACTTC
    CAGGCCATCG AGGAGGTTTA CTACGATCAC CTGCGCAGCG CCACAAAGAA AGGATTTTTC
    AATCTGAATC TGACAAACAG CCCGACCCCG ACGCTGACCT TCTGTGTTAA AACACACGAC
    AGACTGTATT ACATGGTGGC TCCGTCCGCA GAGGCCATGA GGATATGGAT GGATGTGATT
    GTGACAGGAG CCGAGGGCTA CACACAGTTC ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005157509.1
    CDS460..4818
    Translation

    Target ORF information:

    RefSeq Version XM_005157452.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005157452.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGT GTACAGATCT CGACTGGACA GTGATTGCAG CCAATCCTCA
    CTTAGACCGA AAGTCAGCGC CGGTTTGTCT CCTACATACA CGACGGCCAC GCTGGGACGA
    AACAGTCCTG CCAGGAGTCC TCTGATGGTG GCCAACAGCA CCGGAAGTCT CCCACGCAAC
    CTGGCAGCCA CCCTGCAGGA CATCGAGACC AAGAGACAGC TGGCTCTTCA GCAGAAAGGT
    CAGCAGGTTA TCGAGGAGCA GCGCAGGCGT CTGGCGGAGC TGAAGCAGAA AGCTGCAGTG
    GAGGCTCAGT GCCAGTGGGA GGCGCTGCAC GGCTCTCAAA CGCAGCTCCA CCAGCCCTTC
    TCATCAAGCC CAGCACCGGC GCTTCACCAC TCCATCCTGC ACCACACAGC GCCCCCTTGC
    GGAGAGCAGC CGTACGACAC GCTAAGCCTG GAGAGCTCGG ACAGCATGGA CACCAGCATC
    TCTACTGGAA ACAACTCGGC CTGCTCGCCG GACAACATGT CCAGTGCCAG CGGTATGGAT
    GCGTTGAAGA TTGAAGAGAT GGAGAAGATG TTGAAGGAGG CGCAGCTGGA AAAAGCTCGG
    CTCATAGAGT CTAGAGAGCG AGAGACTCAG AGCCGTAAAC TGATGCTGGA GGAGGAGCGG
    AGGAGGAGAG AGGAGGCCGA GAAGAGGCTG CAGGAGGAGA CGCTGCACAG ACAGCAGCTC
    ATCGAGAAGG AGGTCAAGAT GAGGGCCAGA AACTTCTCTC AGGCCCGTCC GATGACCCGC
    TACCTGCCCA TCCGCAAAGA GGAGTTTGAC CTGCGTTCGC ACATCGAGTC TTCAGGCCAC
    AGCGTAGACA CCTGCTACCA CGTCATCCTC ACCGAGAAGA TGTGCAAGGG CTTTCTGGTC
    AAGATGGGAG GCAAGATCAA ATCCTGGAAG AAGCGCTGGT TCGTCTTTGA TCGCCTCAAA
    AGGACGTTCT CTTACTACGC CGACAAACAC GAGAGCAAGC TGAAGGGTGT GATTTACTTC
    CAGGCCATCG AGGAGGTTTA CTACGATCAC CTGCGCAGCG CCACAAAGAA AGGATTTTTC
    AATCTGAATC TGACAAACAG CCCGACCCCG ACGCTGACCT TCTGTGTTAA AACACACGAC
    AGACTGTATT ACATGGTGGC TCCGTCCGCA GAGGCCATGA GGATATGGAT GGATGTGATT
    GTGACAGGAG CCGAGGGCTA CACACAGTTC ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45942
    Clone ID Related Accession (Same CDS sequence) XM_005157452.3 , XM_005157452.4
    Accession Version XM_005157452.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology-like domain family B member 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157452.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGT GTACAGATCT CGACTGGACA GTGATTGCAG CCAATCCTCA
    CTTAGACCGA AAGTCAGCGC CGGTTTGTCT CCTACATACA CGACGGCCAC GCTGGGACGA
    AACAGTCCTG CCAGGAGTCC TCTGATGGTG GCCAACAGCA CCGGAAGTCT CCCACGCAAC
    CTGGCAGCCA CCCTGCAGGA CATCGAGACC AAGAGACAGC TGGCTCTTCA GCAGAAAGGT
    CAGCAGGTTA TCGAGGAGCA GCGCAGGCGT CTGGCGGAGC TGAAGCAGAA AGCTGCAGTG
    GAGGCTCAGT GCCAGTGGGA GGCGCTGCAC GGCTCTCAAA CGCAGCTCCA CCAGCCCTTC
    TCATCAAGCC CAGCACCGGC GCTTCACCAC TCCATCCTGC ACCACACAGC GCCCCCTTGC
    GGAGAGCAGC CGTACGACAC GCTAAGCCTG GAGAGCTCGG ACAGCATGGA CACCAGCATC
    TCTACTGGAA ACAACTCGGC CTGCTCGCCG GACAACATGT CCAGTGCCAG CGGTATGGAT
    GCGTTGAAGA TTGAAGAGAT GGAGAAGATG TTGAAGGAGG CGCAGCTGGA AAAAGCTCGG
    CTCATAGAGT CTAGAGAGCG AGAGACTCAG AGCCGTAAAC TGATGCTGGA GGAGGAGCGG
    AGGAGGAGAG AGGAGGCCGA GAAGAGGCTG CAGGAGGAGA CGCTGCACAG ACAGCAGCTC
    ATCGAGAAGG AGGTCAAGAT GAGGGCCAGA AACTTCTCTC AGGCCCGTCC GATGACCCGC
    TACCTGCCCA TCCGCAAAGA GGAGTTTGAC CTGCGTTCGC ACATCGAGTC TTCAGGCCAC
    AGCGTAGACA CCTGCTACCA CGTCATCCTC ACCGAGAAGA TGTGCAAGGG CTTTCTGGTC
    AAGATGGGAG GCAAGATCAA ATCCTGGAAG AAGCGCTGGT TCGTCTTTGA TCGCCTCAAA
    AGGACGTTCT CTTACTACGC CGACAAACAC GAGAGCAAGC TGAAGGGTGT GATTTACTTC
    CAGGCCATCG AGGAGGTTTA CTACGATCAC CTGCGCAGCG CCACAAAGAA AGGATTTTTC
    AATCTGAATC TGACAAACAG CCCGACCCCG ACGCTGACCT TCTGTGTTAA AACACACGAC
    AGACTGTATT ACATGGTGGC TCCGTCCGCA GAGGCCATGA GGATATGGAT GGATGTGATT
    GTGACAGGAG CCGAGGGCTA CACACAGTTC ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005157509.1
    CDS471..4829
    Translation

    Target ORF information:

    RefSeq Version XM_005157452.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology-like domain, family B, member 1b (phldb1b), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005157452.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTTTGG AGCTTCTGTC CAGAGGATGT GAACAGGTGC TCAAGAATAA CCTCTCTGCG 
    AAGGATTCTC ATGTCCCCGA GCAAGTAAGT GACAGTACAC TGGCCATGGA TACTTTGAAC
    CAAAATCTTA GCCATGGGCA TCAGTCTCAT CAGGTGCTCC AGAGTACTCC ACTGGATCTT
    ATTGAGACTG GAAAGGCACT GAAGGTTCAA GCAGAGCGCC CCCATTTAGT CAGTCTGGGA
    AGTGGGCGTC TCAGTACAGC CATCACCCTC TTACCCCTGC CTGAAGGGAA GACGACTGTG
    GGTCATGGAG CCATGGACAT CAGCATACAG GGCCCCGGGG TCGCAGCTCA GCACTGCTTT
    ATTGAGAACA GGGCTGGAAT CATCACACTT CACCCCTGTG GAAACCAGTG CAGCATGGAC
    GGACTGCCGG TCAACAAACC TGTGCGACTC TCTCAAGGCT GCATGCTGTG TTTCGGCCAA
    TCCGCCTTCT TCCGCTTCAA CCACCCCGAG GAGGCTTTCC GAATGAAAAG CATGATGCCC
    GAGGGAGGAC AGACAGCCAG TGTGAAAAAC TGGACCCACA AAGATTCAGA GAACCTGGTC
    AATGGGAACC ACTCTTCAGG AGAGGCAAAA TCCCACTTGG TTTCCAACGA GAGAGTGCGC
    CCTGAGCACA GTGCCATCGT CAGTTCCATT GAGAAGGACC TTCAGGACAT TATGGACTCA
    TTAGTCATGG ATGACCCTCA GCCCTCATCC TCCGAGTCAA AGAAGCCCTC TAGTCAGTCC
    ATCTCACAGT CTCCCCTTTC TCCGATGTTA AATGGAGGGG GGCGCTTCCT TCTCTCTCCC
    CCAACGAGCC CTGGTGCTAT GTCTGTGGGC TCCAGTTATG AGAATAACTC CCCTCCTTTC
    TCTCCTCTGT CATCTCCCTC GGCTGCCAGC AGTGGCAGCT ACACCACTCT CTCACCAATT
    GGTTGTCAGG ACCAGCCTCA CATGCTTCCT CCTGTGCCAG TGAGGTCTTC TAGTTACAAT
    TATACCAGCC AGCCACCCAT ACCTCAGCCA CGGACCATAC TGCCCAGCTA TTCCAGTGGC
    AGCAGTGGGC CAAAGGTGCC TGAGAGTCCC AGACTCCAGC GAAAGGCGCT TTTAGAAGCA
    CCGCCAAGCC CAAAGCCAAG CCGTAGGGGG TTGAACCAAG ACAGCTCGGT GGCTAAAACC
    CCAGACAGCC CCATACAGAC TCACATTCTG CCCTCAGTCT CCATCTCTAC AGCTCCGACT
    GATGTCCCAT CTGTTAGTCG GGTACCAGTT CCAGGTAGTC CTAGATTAGC CACCAAATTC
    TCCACTGCGT CCTCACCTGC CTCACCCTCT AGTCCCAGAA CCAAAACTAC CATTGTCCTC
    CAAGATCGAC CCTCAAGTCC TTTCCGGGAG CAACCTCAGC CAGATCGTTC TTTGACGTCC
    AGCCCCTCTC ACCAACTCTC CCCACCATCA AGATCTTTCC AGCCCCCATT GGACCCCATT
    GTTCACATCA TTCAAGGTGG ACCTTCACAC CCACATCCTC GAACACTGCA GCCTCCAGAA
    AGTCCGCGGC TGGCCCGCAG AAATCTTGAA GGGAGCAGCA TGAGGGAACT CCCCCCTCTC
    AGTCCCTCCA TAGCTCGCAG GGGGGTTCCT GTGCTCCCAG GTGCTCTACC AGGAACCCTG
    CGAACTCCAG AGAGCCCATC CCCAAGAATG GTGCCAGAGA GTCCCAGACT CAGGAGGAAA
    ACAGGGTCAC CTACAGAGGA GCCATTCAGT CCTCGTGCGA TGCGCACTCG TAGTCCTTCG
    CCCACCTCTG GACTGACTGG CGAAAGCAGT GGACGGAAGG GAAGTTTTGG GAACTCACTC
    TCTTCTGCCT TCAGCCTAAG TTCTCTGCCT GGGTCTTCCC CACGATCTAG TCCCAGGAGC
    CACCGAAAGA TGTCAGCAGG CCACAGAGAC CTCCGGATGC CTCACCCAGG CATGAGGGAG
    CGCAAAAACA GCATCACAGA GATCAGCGAC AACGAGGATG ACCTTCTGGA GTACCATCGA
    CGCCAAAGAG AAGAGCGACT CAGAGAGCAG GAAATGGAGA GACTGGAACG GCAGCGGCTG
    GAGACAATCT TGAACCTCTG CGCTGAGTAT AACAAGGGCG ACGGGCCGCC GGGTGATTTG
    GGCAGGATGG GCGTCTCTGG GCTTGGCTCG AGGGACGGGC TTAACGTCAG GCGTCCTTCA
    ATGGACAGCG TGAACAGCGC TTCACTAAGA GCGACTCAAA GACAACGGGA AAGCCAAGAG
    GACAACCTGA AGGAGGATAG CAGTAGCACT GAGAGTGCGC AGCTGGACGG ACGGACACCT
    GCTCTGCTAA ATGGACAGAA TGGACAGGCT GAGGAGTCTC TGGGTTCGGG CAGCATTGGC
    CGTCTGGAGC TGGGCTACCT GGAGGAGGAG CGGGTTCGAG TGCTGGCAAA GGTAGATGAG
    CTCAAAGCTC GAATCACAGA ACTGGAGCAG CAACTTCAGG AGTCTAAACA AGAGGCGGAG
    ATGGAGAGAG CTCTGCTGCA GGGAGAAAGA CAGGCTGAGA TGGAGCAAAT CGAGACTGAG
    ACCGAGATTA TCAACCAGCT GCAGCGCAAA CTCACTGAAC TGGAGAGCAG CATCCAGAGA
    GAGAAGGACA AGGAGAGGGC TAATGTTGAC GCGGAGCGGG AAGCCCTGGA GAGGCTTCAG
    GATGGGTACA ATGAGTTGAC GAGCCAGCTA CATAACTGCC CTGAGTCTCT GAGGGAACAG
    TTACAGGAAC AGCTCAAGAG GGAAGCTGAG ACTCTTGAAG CAGCGACAAA GCAGTTTGAA
    GACCTGGAGT TTCAGCAGTT GGAGAGGGAG AGCAGTCTGG AGGAGGAGCG CGAGACCATC
    AGCCAGCAGC TCATGCAGGA CAGAGCTCAA TACCACAGCA GTGTCGCCAA GAGAAAGGAA
    AAGGTGGTGG CATTGGAGAG CCAGGCGAGC CAGCTGGGAA TACAGGCCGC TCAGGAGTGT
    GAGAGACTGG CCAAGGACAG AACCATCACC TTGCAGATGC TACACAAGGA AAAGGAAAGG
    CTGGCCAGTC TGGAGAGAAG ATACCAGAGT TTAACTGGTG GAAAGACGTT CCCCAAAAGC
    TCCAATACTA TAAAGGAGGT GTACAGATCT CGACTGGACA GTGATTGCAG CCAATCCTCA
    CTTAGACCGA AAGTCAGCGC CGGTTTGTCT CCTACATACA CGACGGCCAC GCTGGGACGA
    AACAGTCCTG CCAGGAGTCC TCTGATGGTG GCCAACAGCA CCGGAAGTCT CCCACGCAAC
    CTGGCAGCCA CCCTGCAGGA CATCGAGACC AAGAGACAGC TGGCTCTTCA GCAGAAAGGT
    CAGCAGGTTA TCGAGGAGCA GCGCAGGCGT CTGGCGGAGC TGAAGCAGAA AGCTGCAGTG
    GAGGCTCAGT GCCAGTGGGA GGCGCTGCAC GGCTCTCAAA CGCAGCTCCA CCAGCCCTTC
    TCATCAAGCC CAGCACCGGC GCTTCACCAC TCCATCCTGC ACCACACAGC GCCCCCTTGC
    GGAGAGCAGC CGTACGACAC GCTAAGCCTG GAGAGCTCGG ACAGCATGGA CACCAGCATC
    TCTACTGGAA ACAACTCGGC CTGCTCGCCG GACAACATGT CCAGTGCCAG CGGTATGGAT
    GCGTTGAAGA TTGAAGAGAT GGAGAAGATG TTGAAGGAGG CGCAGCTGGA AAAAGCTCGG
    CTCATAGAGT CTAGAGAGCG AGAGACTCAG AGCCGTAAAC TGATGCTGGA GGAGGAGCGG
    AGGAGGAGAG AGGAGGCCGA GAAGAGGCTG CAGGAGGAGA CGCTGCACAG ACAGCAGCTC
    ATCGAGAAGG AGGTCAAGAT GAGGGCCAGA AACTTCTCTC AGGCCCGTCC GATGACCCGC
    TACCTGCCCA TCCGCAAAGA GGAGTTTGAC CTGCGTTCGC ACATCGAGTC TTCAGGCCAC
    AGCGTAGACA CCTGCTACCA CGTCATCCTC ACCGAGAAGA TGTGCAAGGG CTTTCTGGTC
    AAGATGGGAG GCAAGATCAA ATCCTGGAAG AAGCGCTGGT TCGTCTTTGA TCGCCTCAAA
    AGGACGTTCT CTTACTACGC CGACAAACAC GAGAGCAAGC TGAAGGGTGT GATTTACTTC
    CAGGCCATCG AGGAGGTTTA CTACGATCAC CTGCGCAGCG CCACAAAGAA AGGATTTTTC
    AATCTGAATC TGACAAACAG CCCGACCCCG ACGCTGACCT TCTGTGTTAA AACACACGAC
    AGACTGTATT ACATGGTGGC TCCGTCCGCA GAGGCCATGA GGATATGGAT GGATGTGATT
    GTGACAGGAG CCGAGGGCTA CACACAGTTC ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.