gRNAs
The following smg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ODa03664 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001080044.2 | |
| Accession Version | NM_001080044.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 10923bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-04-07 | |
| Organism | Danio rerio(zebrafish) | |
| Product | serine/threonine-protein kinase SMG1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM986821.1. On Jun 24, 2009 this sequence version replaced NM_001080044.1. ##Evidence-Data-START## Transcript exon combination :: FM986821.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001073513.2 |
| CDS | 179..11101 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001080044.2 |
1 | ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa45856 | |
| Clone ID Related Accession (Same CDS sequence) | XM_009299453.2 , XM_009299453.3 | |
| Accession Version | XM_009299453.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 10926bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-23 | |
| Organism | Danio rerio(Zebrafish) | |
| Product | serine/threonine-protein kinase SMG1 isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_009297728.1 |
| CDS | 175..11100 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_009299453.2 |
1 | ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa45856 | |
| Clone ID Related Accession (Same CDS sequence) | XM_009299453.2 , XM_009299453.3 | |
| Accession Version | XM_009299453.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 10926bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-06-14 | |
| Organism | Danio rerio(zebrafish) | |
| Product | serine/threonine-protein kinase SMG1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299453.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_009297728.1 |
| CDS | 181..11106 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_009299453.3 |
1 | ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa45857 | |
| Clone ID Related Accession (Same CDS sequence) | XM_009299454.3 , XM_009299454.2 | |
| Accession Version | XM_009299454.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 10923bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-06-14 | |
| Organism | Danio rerio(zebrafish) | |
| Product | serine/threonine-protein kinase SMG1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299454.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_009297729.1 |
| CDS | 181..11103 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_009299454.3 |
1 | ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa45857 | |
| Clone ID Related Accession (Same CDS sequence) | XM_009299454.3 , XM_009299454.2 | |
| Accession Version | XM_009299454.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 10923bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-23 | |
| Organism | Danio rerio(Zebrafish) | |
| Product | serine/threonine-protein kinase SMG1 isoform X2 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299454.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_009297729.1 |
| CDS | 175..11097 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_009299454.2 |
1 | ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
DHX34 and NBAS form part of an autoregulatory NMD circuit that regulates endogenous RNA targets in human cells, zebrafish and Caenorhabditis elegans. |
Processing of fish Ig heavy chain transcripts: diverse splicing patterns and unusual nonsense mediated decay. |
Nonsense-mediated mRNA decay effectors are essential for zebrafish embryonic development and survival. |
Chk1 suppresses a caspase-2 apoptotic response to DNA damage that bypasses p53, Bcl-2, and caspase-3. |
