smg1 cDNA ORF clone, Danio rerio(zebrafish)

The following smg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa03664 NM_001080044.2
Latest version!
Danio rerio SMG1 nonsense mediated mRNA decay associated PI3K related kinase (smg1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45856 XM_009299453.3
Latest version!
Danio rerio SMG1 phosphatidylinositol 3-kinase-related kinase (smg1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45857 XM_009299454.3
Latest version!
Danio rerio SMG1 phosphatidylinositol 3-kinase-related kinase (smg1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa45857 XM_009299454.2 Danio rerio SMG1 phosphatidylinositol 3-kinase-related kinase (smg1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45856 XM_009299453.2 Danio rerio SMG1 phosphatidylinositol 3-kinase-related kinase (smg1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa03664
    Clone ID Related Accession (Same CDS sequence) NM_001080044.2
    Accession Version NM_001080044.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-07
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase SMG1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM986821.1. On Jun 24, 2009 this sequence version replaced NM_001080044.1. ##Evidence-Data-START## Transcript exon combination :: FM986821.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG 
    AATGACTGGC AGCCCAGAAG CGACAGTCTG TCAGCCAGCC AGGACGGTGT GAAGTGCAGT
    GTCTCTAGAG ACCGCGGTTC AGAAGTTCTA TTTGAAATTC TGCCCTCTGA CCGGCCTGGC
    ACCCCACCAC TGCACAATGA CGCCTTCACT GCCACCGATG CACTCGAAGA GGGAGGTGGA
    TACGACGCTG ATGGAGGCCT CTCTGAAAAT GCTTATGGTT GGAAGTCGCT GGGGCAAGAG
    TTGAGAATTA ATGATGTGAC TACTGATATA ACCACTTTTC AGCATGGAAA CCGTGCTTTA
    GCTACAAAGG ACATGAGGAA ATCACAGGAC CGACCGTTGG CTCACTCTGA GGAGTCTCGT
    CTGGCCAATC TTCTGCGGAG AGCATCACGG GAAGATGACC GGGAACGCAG ACTGGCCACT
    ATGAAACAAA TGAAGGAGTT CATTGTGCAC TCTGAAAATA AAGTGGTCCT AGTGAAACAG
    TTGGATTCCA TTTTGAGCAC CCTGAATGAC ATTTTGAATG AGAGTAGTAA GCTCCTGCAG
    GAGCTCAGAC AGGAGGCCGC TTGGTGTCTG GGATTGCTCT GTGCCGCCCT CAGTTATGAA
    GCTGAGAGGA TCTTCAAGTG GATGTTCCTC AAGTTCAGTG TGTCTACAAA GGATGAGGTG
    AAGCTTCTGT ACCTTGTGGC AGTGCACAAA GCACTTGAAA CAGCTGGGGA GAAGAAGGCC
    TTTTCAGCTG TAATGCAGCT GGTGATGAGC AACCTTCAGT CTATATTGGA GAACTTGGAC
    ACACCGGAAC TATTGTGCCA AAGTGTAAAG TGTATTTTAC TGGTGGCCCG TTGTTATCCA
    CACATCTTCA GCACCAACTT CAGAGACACA GTGGATATCT TGGTAGGCTG GCATATAGAT
    CACACACAGA AGCACTCTCT TACTCAACAA GTATCTGGCT GGTTGCAGAG TTTGGAGCAA
    TTTTGGGTTG CAGATTTGGC CTTCTCGACC ACATTATTGG GTCAGTTTTT GGAAGATATG
    GAGGCATATG CTGAGGACCT GAGCCATGTT GTATCTGGAG AATCAGGAGA TGAGGACATT
    CCCCCTCCCA CTGTGTCCTT GCCTAAATTG GCAGCCCTAT TGAGAGTTTT TAGCACTGTT
    GTGCACAGCA TCGGAGAACG GTTTAACCCA ATCCGAGGCC CTCCAATTAC AGAAGCATAT
    GTCACTGATG TTTTGAATCG TGTTTTGGCT TGCGTCACCA CAGCCAAGCA GGTGTTCTTC
    TCTGAGGCGG TGCTGACAGC AGGAAATGAG TGTGTGTGTG TTCTGCTGGT GAGCATAGAC
    TTGGGCGGCC AGCTGATTGA TGCTGTAATC TCATACGGCC TAGACCAGCT GAACTGCTGC
    CAGAACTGCG GACCCGAATA CAGCCTGTCT GTTCTCACCC TGCTCACACT GGTTGTTGAC
    CAAATAAACA CCAAACTTCC GGCTTCTTTT GTGGAAAAGT TGTTGGCCCC AAAATCTCAT
    CTTTTGGAGC TGCGCTTTCA CAGAGAGAGA GAGGTGATGG CGGCAGCACA TGGAGTGTAT
    CATGCTGTGC TAAGCCTGAA GAACATCCCT ATCTTGGAGG CGGCATATAA ACTGGTCTTG
    GGCGAGATGG GCTGTGCACT GAACAGTTTG CTGAGCCCTC TGGGTTTGCC TGATGCCTGT
    CCTCACATCC AACATCCTGC TTTCAGCCAG CTGAACTTTA GCCCAGAGAG AGCTGAGTTT
    GTCCTCATCT TTAACCTCAG TGCTCTCACC ACCATTGGCA ACACCAAGAA CTCCCTCATC
    GGGATGTGGG CTCTGTCTCC TACTGTGTTT GCCCTGCTCA GTCAGAATCT GGTTATTGTT
    CATTCTGATT TGGCAGTTCA TCATCCTGCT GTCCAGTATG CAGTGCTCTA CACTCTCTAC
    TCTCACTGCA CCAGACATGA TCACTTCATC TCCAGCAGCC TGAGCTCCTC GTCCCCATCC
    CTTTTCGACG GTGCTGTCAT CAGCACAGTT ACCACGGCAA CCAAAAGGCA TTTCAGCACG
    CTCCTTAATT TGCTGGGTAT GCTGCTCAGC AAGGATCATC TCAACCCTGA GGCGAGGCGG
    CTGTTACTCA CCTGGTCATT GGAAGTAGCT CTGATGATGA AGAAATCTGA AACTTACGCT
    CCTCTCTTCT CGCTCCCATC CTTCCTCAAA TTCTGCAAAG GGTTACTGGC AAATTCCTTG
    AATGAGGACA CCACCATATG TCTGCAGGCA TGTAACAGTC TACAGGTGCT GTCATCTTCC
    TTGACTATGG AGCTTCTCCA GAGGTGTGTG GACGTGTGCC GAGTTCAGCT GGTTCACTCA
    GCAGTCCGAG TCCGGCAGGC CTTTGGGAAA TTGCTGAGAT CTGTTCCCAT GCACGTCGCA
    CTGAGCGCCC ATAGTCACTC TGAGATTAAG GAGATTTCTC TGGCCATCCG CCGACACATG
    AGCAAAGTGC CTAGCAACAC ATTCCACCCA CAGGACTTCT CTGACCTTAT TGGGTTTATT
    CTGTACGGAA CGGTCCATCG TGGGGGGAAG GAGCCCTGGT TGGAGCGTCT GTATCACAGC
    TGTCAGCGGC TGGAGAAGAA AGACTCTGCT ATGGTGCCTC GTGCCCTGCT GAAGACTGAG
    GCAGTGCTGT GGCAGTGGGC GGTGTGGGAA GCTGCTCAGT TTACTGTTCT CTCAAAGCTC
    CGTACACCTC TGGGAAGAGC CCAGGATACC TTCCAGACTA TAGAGGGTAT GATCCGTAGT
    CTGGCAGCTC ACAGTCTGAA CACAGAGCAG GAGCTGAGTC AGTGGAGCGG AGGAGAGAGT
    GATGAGGGTC ACCACACCAA CCAGCTCCGA CTGGCTCTTC TCCTGCAGTT CCTGGAGAAC
    CTGGAGAAAC TCATGTACAA CGCCTATGAA GGCTGTGCCA GTGCTCTCAC TGCACCACCA
    AAGGGCATTA GAACCTTTTT CTACACAAAC AGACAGACAT GCCAGGACTG GCTAACCAGA
    ATTCGTTTGG CCCTAATGAG GGTTGGCCTT CTCTCCGGTC AGCCAGCGGT CACAGTCCGT
    CATGGCTTTG ACCTGCTGAC TGAGATTAAA AACAGCAGTA CACAGGGTCC AGAAATGGAG
    GTTCCAGTGA CCATGCTGGT GGAGGCATTG TGCGAGTTGA GATGCCCAGA GGCTATTCAA
    GGTCTGGCAG CATGGAGTCT TGCCAACACT GGCAAAAGCA TGGGCTGGCT GAGCTCTGTG
    GCACTGCAGG CTGAGGGAAA GTTTGAGAAG GCAGCGTTAG AGTACCAAGA GCAGCTTTGT
    GCTGTCACAG GGATGGACTG CTCCATTAAA GTCTTTGATC GCTCACTTTT GAAACTCACT
    GGCACCAACA CCTCCAGTCC AAAACATACC AGCAGCGGAG AGGGAAGAAA AACTGTATTG
    CTGAAATCCA GCGAGTGTTC TCCAGAGGTT CTGAACTTTC TGGCTAATAA GGCCTGTGAG
    TGTTATGTGG CCCTGAGCGA TTGGGAGTCT GTGCAAGAAT GGCAGGCCAG CATGATGAAT
    CTCAAAAAGA ACAGCAGCAG TTCCAGTGTC AACCTCAAGA CAGACTTCAA CTACATCAGG
    TCTATGAGCA GGTTTGAGGA GGGAGATTTT ACAGAATGCC GTGCCCAGCT AGAGTTGCTC
    CCTGGGGATG ATTATGGCCT TCTCAACTCT ACAACTAAGG ACAAGATTGA CCTTAAGAGG
    CTTCTTCCTG CTGTGTTGAG TCCAGACCCA TCAGAGCTCC AGAAGGCCAT TGAGGTGCAG
    CTCCTCAGAA GTGCTGTGGG AGCCATCAGT GCGACCAATC ATGAGCAGGA CCAGAAAGTG
    GCATCATCAG ACACCCTGGT TAAATATCTG AAGCAGACGG GTCGCATCTG TCTGGGCCCC
    CTGCGCCTTT CCACTCTCAC CCTGTCAGAT TCCCTGCCCA CCCTTAGCAC TCTACAGCTG
    CACTGTGCCA ACTCTTTAGA GAACTCTCTT TGCAACCAAC ATCCTGAGGA CTGCCTTATT
    CCGCTCTTCA GTGAAGCTTT GACCACCTGC AAGCAGCAGG ATGTTCAACC ATGGCTGCAT
    GCTCTCCGAT ACACTACTTT TCAGCGGGAA TTCTTCCAAA AACTTAAAGG TTCATCTTCT
    CCAGTTGATT CTCATCTGAT GGAGCTTTGT CTGACTGCTG TAAAATTTGC TCGTAAACAA
    GGGAATATTG CTCTGGCCAC ACGCCTGCTG AGTCTATGCA GCAAGCCAGC TATGAGCGAC
    ACTGAAGGTC AGGACCTGGT TCAAAGCTTC AGGCAGCTGT CTCTAGAAGG CACTGTTGGG
    GAAAAGTGGG GACCAGAGCT GGAGATCGAG AAGGCTAAAG TTCTGTTCGC TGCTGGTCAG
    TCTGTGTCTG CTATGGAGAT GTTGAGCTCC TGTGCACTGT CATACTGTCA CTCTGGGAAG
    TGCGAACTTG CGGCCTGTCG CTCGGTGCTC ACTCTGTGCA AATGGCTTTT GGCTGACTGG
    AAAGATCTGA CTCCCCAGCT GAAGATGGTG GTGAAGAAGA ACTCTGGGTC TACCAGCCTC
    TCAACTCTCT CCAAAAACAT CTCTGGCTTG TTGGAGCTGC CGCTGGAAGA TCAGGGAATG
    CCACACATTA CAACAGAGAC TACAGTTAGC GTTGGTGTCG GGGAGCCAGA CTTTGTCCTT
    GGGCAACTTT ACCAGCTCTC AACTACTCAG GCTCCTGAAG TGGCCAAATC TTGGGCTGCT
    CTTGCCAGCT GGGCCTACAG ATGGGGCAGG AAGGTTGTGG ACAATGCCAG TCAAGGAGAA
    GGGGTTCCTC TGCTCCTCGG AGAGAAGAAA GAAATAGAGG AGTTGCTCCC AGCAGGCACC
    AGCGATGAGG ACAAGGAAAC CATTTTTGGC ATTTTGGGTC AAGCCATGTG TCGCCCAGCA
    GGAATTCAGG ATGAGGATAT GGCTCTGCAG AATGAAGAAG ATGATGAGGA CGACATGGTA
    GATGTAATTG GCCGACAGCT GTTGGGTGCC TGTCCCTGGC TTTCAGATGT AGAGGACACT
    GTGACCGATG GACTCATTGG GGTGTGGAGG AGAGTGGTTG ATCGGATCTT CAGCCTCTAC
    CGGGTCTCCT GCAGGGCTTA CTTCACTTTC CTCAAACTCA ATGCTGGACA GGTGCCTATA
    GATGAAGATG ATCCCAAGCT GCTCCTGAAC AACCAGAACA GCAAGCAGAG CAGCGATGAT
    GTCATTGTCA TGGCAACCCT CCGTCTGCTG CGCTTGTTGG TGAAGCACGC AGGAGAGTTG
    AGGGAAGGTC TGGAGCACGG TTTGGCATCC ACACCTACTG CTCCCTGGAG AGGTATTATC
    CCTCAGTTAT TCTCCAGACT TAATCACCCT GAAGCCTACA TCCGCCAGAG CATCTGCAGT
    CTGCTGTGTA GAGTGGCCCA AGATTCCCCA CACCTTATCC TTTATCCTGC CATTGTCGGA
    TCAATCTCTC TTGGTGGAGA GGCTCAGACG GCAGGTAATA AGCTGCCATC GTCCCTGCCA
    ACCCTGCTGG GGAACATGCA GGGAGAGGGT CTTTGTGGAG GTGAGAGCGA GACAGGCAGT
    GGGCCGACCT CTCAGGAGAG CAGCCGCGGG GAAGAGATGG TGATGTATTC CAGCGAGGAT
    CAAGCCATGA TGCAGGACTG CTACAGCAAG ATAGTAGACA AGCTTTCCTC TGCCAACCCT
    ACCATGGTGC TGCAGGTGCA GATGTTGGTG GGCGAGTTGA GGAGGGTCAC ACTGCTCTGG
    GACGAGCTCT GGCTGGGGGT CTTACAGCAG CAGCACATGC ACGTCCTGCG CAGGATTCAA
    CAGCTGGAGG ACGAGGTGAA GAGAGTTCAG AACAACAACA CTCTGCGCAA GGATGAGAAG
    GTGGCTATTA TGAGAGAGAA GCACTCTGCT CTGATGAAGC CTGTGGTTTT TGCGCTGGAT
    CATGTGCGCA GCATTACGGC TGCTCCAGCA GAGACGCCTC ACGAGGAATG GTTTCAGGAG
    ACATATGGAG ATGCCATCCA CAATGCCCTA GAGAGACTGA GGAGCCCTCT AAATCCAGCC
    AATCCTGCCA GCAGCTGGGT GCCCTTTAAA CAGATCATGC TGAGTTTACA GCAGAGGGCA
    CAGAAACGAG CCAGTTACCT TCTGCGTCTG GATGAGATCA GCCCACGTCT GACTGCTATG
    GCTAACACCG AGATGGCTCT GCCGGGAGAG GTGTCTGCTA CAGATGCAGT CACCATCCAG
    AGTGTGGGCA ACACCATCAC CATTCTGCCA ACTAAAACCA AACCCAAGAA GCTCTACTTT
    TTGGGATCGG ATGGGAGAAA CTATCCTTAT CTGTTCAAAG GATTGGAGGA TCTGCATTTG
    GATGAGAGGA TCATGCAGTT CTTGTCTATC GTCAACACCA TGTTCACTAA AGTGAATCAG
    CAGGAGAGCC CGAGGTTTCA AGCCCGTCAT TATTCTGTCA CACCTCTGGG TACCAGATCT
    GGCCTCATCC AGTGGGTGGA TGGAGCCACT CCACTGTTTG GCCTGTACAA ACGTTGGCAG
    CAGCGTGAAG CTGTGGTCCA AGCCCAGAAG GCACAGGACT CCTTCCAGCA GCCTCAGAAT
    CTTCCCATGG TCCCACGGCC CAGTGAGCTT TACTACAGTA AAATCAGTCC TGCCTTAAAG
    GCTGTCGGTC TCAGTTTGGA TGTGTCACGT CGTGATTGGC CGTTAAGTGT TATGAGAGAT
    GTTCTAAGAG AGCTGATGGA AGCCACGCCC CCTAACCTCT TGGCAAAGGA GCTCTGGTGT
    TCCTGCACCA CTCCTAGTGA GTGGTGGAGC GTCACTCAGA CTTACGCCAG ATCCACCGCT
    GTGATGTCAA TGGTGGGATA CATTATTGGC TTGGGGGATC GTCACCTTGA CAACGTGCTG
    ATTGACATGA CGACTGGCGA GGTGGTGCAC ATTGACTATA ACGTCTGTTT TGAGAAGGGA
    AAAAGCTTGA GAGTGCCTGA GAAAGTTCCT TTTCGCATGA CCCACAACAT CGAGACTGCT
    CTGGGGGTCA CAGGAGTTGA GGGAATCTTT AGACTGTCCT GTGAACAGGT GGTCCAGATA
    ATGCGGCGTG GGAGAGAGAC CCTCTTGACT CTTCTAGAGG CCTTTGTCTA TGACCCATTG
    GTGGACTGGA CAGCAGGCGG TGAGGTGGGC TTTGCAGGTG CTGTGTATGG AGGAGGTGGT
    CAGCAGGCTG AGAACAAGCA GAGCAAGAGA GAGATGGAGA GAGACATCAC ACGTAGCCTT
    TTCTCCTCCC GTGTGGCTGA AATTAAGGTG AACTGGTTCA AAAACCGTGA TGAAATGACT
    GGAGTGTTGC CACAGCTGGA GGAGGCAGTG GATGAGTACC TCAACCTGCA GGAGCAGCTC
    ACACAAGTGG AGAAGGTGCA GGGCAAGCTG TTGGAGGAGT TGGAGTTCCT GGAGGGAGCA
    GACACCAGAG CAGACCATCC CATCCACTCC CTGGAGCACA GATACTCAGA ACACACGCAG
    CTGCAGTCCA GACAGAGAAC TGTGCAGGAT GCCATTCAAG GGAAGTTGTC AGATCTGGAT
    CAATGGATCT CTCAGTATCA AGCTGCCTTT GCCAGTTTAG AAGCCACACA GCTTGCCAGC
    CTCCTCCAGG AGATCAGTAG CCCTATTGAT TTGGGTCCGC CCAGTTATGT ACCAGCAACG
    TCGTTCTTGC AGAATGCAGG TCAGGCACAT CTGATCAGCC AGTGTGAAGC GCTGGAGGCT
    GAAGTGAGTG CTCTGTTACA GCAGAGGCGC TCTCAGCTGC GAGGTTGTCT TGAGCACCTG
    CACAGTTATG CCACCGTGGC CCTGCTGTAC CCACGTGCCG TGCTGCACAG ACACAGAGCT
    CACACATGGA AACAGTGGAT GGAGGAGCTG GTGTGTGACA TGACGGTCGA TCACTGCCAG
    ACTATTTATC ACCAATACGA AATGCAGTTT GCACCCCAGC CCCCACCTGC CACGTGTCAG
    TTCCTCTCCA GCATCGAGAT GGCGCTGCAG CACCATGCAG CAGAAACCAA CACTCGTCTG
    CTGCGGCAGG TGGAGCGTCT GAAGGCAGAG GGCGCCAGTG TACCCGTGTG TGAGGAGCAG
    CTGCAGGAGA TCGAGCGCTG CATCAAAGTC TTCCTGCACG AGGACGCTGA GCTTGGCTCC
    TTCAGCCTGG CTGGCATCAT CGTCTCTGCC CTCTGTTCTC TCACCAGGCG TAATTTGGTG
    ATGGAGGGAG CTGCGGCCAG TGCTGGCGAG CAGCTGGTGG AGCTGACGTC TCGTGATGGA
    GCCTGGTTCC TAGAGGAGCT CTGCAGCATG AGTGGAAACA TTACATGTCT TGTGCAGCTG
    TTGCAGCAGT GCCAGCTTCT GTCACATGAC CTGGACATCC CTAGTCCTGC AGAAACTTCG
    CAAGTTGTCT ACCTGACCAA TGGTGTTTAC ACCTGTCTTC AGGAGCTGAA CACAAACTTC
    CGTCAGATCA TCTTCCCAGA GGCTCTGCGC TGCATGCTGA AAGGTGAAAA CACTTTAGAG
    ACCATGTTAG CAGAGCTGGA TGCGCTTATC GATCAGTGTG CAGATGGGGT TTCACTGCAG
    GGGCTTGGAG AGATTTTGAT GGCACACATT CGGAACGCCT CCATGGGTCT GGAAGAGGAT
    CCAGATGACC ACTATCTGGA CGTTACCAGG GTTTTGCGAG CCCAGTACTC TGAGCTGATT
    CAGCCCCGCA GCATGGAGAG CTCAGTGCAG GAAACGCCAA AGATGAGCGC TGGTCAGATG
    TTGCTGGTAG CTTTTGATGG CATGTTTGCC CAGTTGGAGA CAGCTTTTGG ACTTCTCATT
    GACAAGCTGA ACTCTATGGA TGTCCCTGCT GCCTGGAGAA AAGTGGATGT GATCCGGGAG
    TCGAGAGCCA CACAGGCTCA TTTCTTTGAT AATGTCCAGA CCAGGCAGGT CTTGGAGGAG
    ATTTTCTTCT TGAAGAGACT CCAGACCATC CGAGACTTCT TCCGTCTATG TGGTTCATTT
    GCACAGACAC TATCTGGCAC ATGCCCTACA CCCACTGATG ACCCTCCGCC ATCTAATGGC
    CCTGTTCCCA TAGTAAAGCC CTTGTATCGA GGCTCCACGG TGGTGAGCGA GGACCAGATG
    ACGCGGCCCA TCAAAGCCTT TACAGCAGAC TTTGTAAGGC AGATGCTGAT GGGGCTCCCC
    ACCCAGGCTC TGGGTCTAGC CATCTGCAGC TCTCTCAGTG CCCTGGGCAT GGATCTTATT
    GCTCAAGTGG AGGCCAAGGA CTTTGGAGCT GAGGGAAAAG TCTCTCTGGA TGACCTGTGC
    AAGAAAGCTG TAGAGCAGGG GGTGCAGGCA GGACGCCTGT CCCAGCTGCT ACTCAACAGG
    GCCACCGTGC TGGCCAGTTC CTATGACACT GCCTGGAAAA AACTTGACCT GGTCCGACGT
    CTGGAGGTCA GCATTGAAGC CTGTAAGGTC AGCCTGCAGA GGAATCAGCT TCACATCGCC
    ATGTTCCAGT GGCAACATGA GGACATTCTT GGTGCGAGGA CGCAGCCCAT GACAGTCAGT
    CCTCCACCAC GTTCCATTAT CCTCAGCAAC ATGAAAAAGA AGCTCTACAA ACTCAGTCAG
    GATGACGCTT CAATAGGAAG TGTCCAGGAG AAGTTGGCCA GTCTTGAAGG CAGTATAGAG
    CAGAGGCTGA AATGGGCCGG TGGTGCAAAT CCTGCACTCG CTCCAGTGCT GCAGGACTTT
    GAGGCCACTA TAGCAGAACG CCGTGCCTTG GTGATAAAGG AGAGTCAGCG TGCCAACCAG
    GTCACTTTCC TCTGCAGTAC CATTCTCAAC TTTGAAGGTC TGCGCACCCG CACACCCGAA
    GCTTTGAACA TGGATGCTGC CCTTTTTGAG CTTGTGAAAC GATGCCAGGC CACCTGCTCC
    TACGCTGCCC AGTTCAATAC CTCTGTTTCC TCGCTAGAAC TACAGCTGCT GCATAGACTG
    AGTCCTGCCA TGGATATGTC CATTGGTGGT CCTGAGTGGC TGGTGTATGC ACAGAATCAC
    CTGACCCAGG AGATGTCCAG TCAGAGAGCC ATGCAGGAGG AAAGAGAGCA GCAGCTGGAG
    AGCGTGACTG AGACCCTGCA GCTGCTGGTG GACTCCATTA AAGGCACCCT GTCCAATCAC
    AACCGCCAGC TCGCTGACGT TAAACACTTA CTGCGAGCAA TGGCAAAGGA TGAAGAGAAT
    GCCCTTGCAG AAGGAGAGGA AGTGACTTAT GATGGGAGTG TGCGTCAGTT CCTTTCTGAA
    TACAAGGCAT GGCAGGATAA TGTGCAGATA GTGCTGTTCA CAGTAGTGCA GGCTACAGGG
    CAACCACGCA GTCAAGAGCA GATCGAGCTG CTGCAGGAGA TTCCCGCCAC CTTGAAGGAG
    CTTAAGGTTC AGAGCCACAG CATCTACAAT GGATTGGTGG GCTTTGCCTC TCCCCTTGTG
    ACAGAGAGAG GAAGTGACTG TATTAGTCCC ACTTCCACAG TCCAGACCAG CTTTGCCGCT
    GCAGTGCGCT GCAGTGGGGT GAAGACACAG CCTGACAGTA TGTCTCAGAA TGCACGCAAA
    GCTCTTCCTC GAAACTTTGG GACCCCGGCG GACACTCCTC CCAGCACACT GATGATCAAC
    AGCAAAGGAC TGGCCCCTAG CCCCAAAAGA GCCGTGCGAG ATCCCAAAAC TGGGAGAGCT
    GTGCAAGAGA GAAATTCATA TGCTGTTAGT GTGTGGAAAA GGGTGAAGGC CAAGCTGGAG
    GGGAGAGATG TGGATCCTAA CAGGAGAATG AGTGTCACTG AGCAGGTGGA TTATGTCATC
    AAAGAGGCAA CAAACGTGGA CAACCTGGCA CAGTTGTACG AGGGATGGAC GGCATGGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001073513.2
    CDS179..11101
    Translation

    Target ORF information:

    RefSeq Version NM_001080044.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio SMG1 nonsense mediated mRNA decay associated PI3K related kinase (smg1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001080044.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG 
    AATGACTGGC AGCCCAGAAG CGACAGTCTG TCAGCCAGCC AGGACGGTGT GAAGTGCAGT
    GTCTCTAGAG ACCGCGGTTC AGAAGTTCTA TTTGAAATTC TGCCCTCTGA CCGGCCTGGC
    ACCCCACCAC TGCACAATGA CGCCTTCACT GCCACCGATG CACTCGAAGA GGGAGGTGGA
    TACGACGCTG ATGGAGGCCT CTCTGAAAAT GCTTATGGTT GGAAGTCGCT GGGGCAAGAG
    TTGAGAATTA ATGATGTGAC TACTGATATA ACCACTTTTC AGCATGGAAA CCGTGCTTTA
    GCTACAAAGG ACATGAGGAA ATCACAGGAC CGACCGTTGG CTCACTCTGA GGAGTCTCGT
    CTGGCCAATC TTCTGCGGAG AGCATCACGG GAAGATGACC GGGAACGCAG ACTGGCCACT
    ATGAAACAAA TGAAGGAGTT CATTGTGCAC TCTGAAAATA AAGTGGTCCT AGTGAAACAG
    TTGGATTCCA TTTTGAGCAC CCTGAATGAC ATTTTGAATG AGAGTAGTAA GCTCCTGCAG
    GAGCTCAGAC AGGAGGCCGC TTGGTGTCTG GGATTGCTCT GTGCCGCCCT CAGTTATGAA
    GCTGAGAGGA TCTTCAAGTG GATGTTCCTC AAGTTCAGTG TGTCTACAAA GGATGAGGTG
    AAGCTTCTGT ACCTTGTGGC AGTGCACAAA GCACTTGAAA CAGCTGGGGA GAAGAAGGCC
    TTTTCAGCTG TAATGCAGCT GGTGATGAGC AACCTTCAGT CTATATTGGA GAACTTGGAC
    ACACCGGAAC TATTGTGCCA AAGTGTAAAG TGTATTTTAC TGGTGGCCCG TTGTTATCCA
    CACATCTTCA GCACCAACTT CAGAGACACA GTGGATATCT TGGTAGGCTG GCATATAGAT
    CACACACAGA AGCACTCTCT TACTCAACAA GTATCTGGCT GGTTGCAGAG TTTGGAGCAA
    TTTTGGGTTG CAGATTTGGC CTTCTCGACC ACATTATTGG GTCAGTTTTT GGAAGATATG
    GAGGCATATG CTGAGGACCT GAGCCATGTT GTATCTGGAG AATCAGGAGA TGAGGACATT
    CCCCCTCCCA CTGTGTCCTT GCCTAAATTG GCAGCCCTAT TGAGAGTTTT TAGCACTGTT
    GTGCACAGCA TCGGAGAACG GTTTAACCCA ATCCGAGGCC CTCCAATTAC AGAAGCATAT
    GTCACTGATG TTTTGAATCG TGTTTTGGCT TGCGTCACCA CAGCCAAGCA GGTGTTCTTC
    TCTGAGGCGG TGCTGACAGC AGGAAATGAG TGTGTGTGTG TTCTGCTGGT GAGCATAGAC
    TTGGGCGGCC AGCTGATTGA TGCTGTAATC TCATACGGCC TAGACCAGCT GAACTGCTGC
    CAGAACTGCG GACCCGAATA CAGCCTGTCT GTTCTCACCC TGCTCACACT GGTTGTTGAC
    CAAATAAACA CCAAACTTCC GGCTTCTTTT GTGGAAAAGT TGTTGGCCCC AAAATCTCAT
    CTTTTGGAGC TGCGCTTTCA CAGAGAGAGA GAGGTGATGG CGGCAGCACA TGGAGTGTAT
    CATGCTGTGC TAAGCCTGAA GAACATCCCT ATCTTGGAGG CGGCATATAA ACTGGTCTTG
    GGCGAGATGG GCTGTGCACT GAACAGTTTG CTGAGCCCTC TGGGTTTGCC TGATGCCTGT
    CCTCACATCC AACATCCTGC TTTCAGCCAG CTGAACTTTA GCCCAGAGAG AGCTGAGTTT
    GTCCTCATCT TTAACCTCAG TGCTCTCACC ACCATTGGCA ACACCAAGAA CTCCCTCATC
    GGGATGTGGG CTCTGTCTCC TACTGTGTTT GCCCTGCTCA GTCAGAATCT GGTTATTGTT
    CATTCTGATT TGGCAGTTCA TCATCCTGCT GTCCAGTATG CAGTGCTCTA CACTCTCTAC
    TCTCACTGCA CCAGACATGA TCACTTCATC TCCAGCAGCC TGAGCTCCTC GTCCCCATCC
    CTTTTCGACG GTGCTGTCAT CAGCACAGTT ACCACGGCAA CCAAAAGGCA TTTCAGCACG
    CTCCTTAATT TGCTGGGTAT GCTGCTCAGC AAGGATCATC TCAACCCTGA GGCGAGGCGG
    CTGTTACTCA CCTGGTCATT GGAAGTAGCT CTGATGATGA AGAAATCTGA AACTTACGCT
    CCTCTCTTCT CGCTCCCATC CTTCCTCAAA TTCTGCAAAG GGTTACTGGC AAATTCCTTG
    AATGAGGACA CCACCATATG TCTGCAGGCA TGTAACAGTC TACAGGTGCT GTCATCTTCC
    TTGACTATGG AGCTTCTCCA GAGGTGTGTG GACGTGTGCC GAGTTCAGCT GGTTCACTCA
    GCAGTCCGAG TCCGGCAGGC CTTTGGGAAA TTGCTGAGAT CTGTTCCCAT GCACGTCGCA
    CTGAGCGCCC ATAGTCACTC TGAGATTAAG GAGATTTCTC TGGCCATCCG CCGACACATG
    AGCAAAGTGC CTAGCAACAC ATTCCACCCA CAGGACTTCT CTGACCTTAT TGGGTTTATT
    CTGTACGGAA CGGTCCATCG TGGGGGGAAG GAGCCCTGGT TGGAGCGTCT GTATCACAGC
    TGTCAGCGGC TGGAGAAGAA AGACTCTGCT ATGGTGCCTC GTGCCCTGCT GAAGACTGAG
    GCAGTGCTGT GGCAGTGGGC GGTGTGGGAA GCTGCTCAGT TTACTGTTCT CTCAAAGCTC
    CGTACACCTC TGGGAAGAGC CCAGGATACC TTCCAGACTA TAGAGGGTAT GATCCGTAGT
    CTGGCAGCTC ACAGTCTGAA CACAGAGCAG GAGCTGAGTC AGTGGAGCGG AGGAGAGAGT
    GATGAGGGTC ACCACACCAA CCAGCTCCGA CTGGCTCTTC TCCTGCAGTT CCTGGAGAAC
    CTGGAGAAAC TCATGTACAA CGCCTATGAA GGCTGTGCCA GTGCTCTCAC TGCACCACCA
    AAGGGCATTA GAACCTTTTT CTACACAAAC AGACAGACAT GCCAGGACTG GCTAACCAGA
    ATTCGTTTGG CCCTAATGAG GGTTGGCCTT CTCTCCGGTC AGCCAGCGGT CACAGTCCGT
    CATGGCTTTG ACCTGCTGAC TGAGATTAAA AACAGCAGTA CACAGGGTCC AGAAATGGAG
    GTTCCAGTGA CCATGCTGGT GGAGGCATTG TGCGAGTTGA GATGCCCAGA GGCTATTCAA
    GGTCTGGCAG CATGGAGTCT TGCCAACACT GGCAAAAGCA TGGGCTGGCT GAGCTCTGTG
    GCACTGCAGG CTGAGGGAAA GTTTGAGAAG GCAGCGTTAG AGTACCAAGA GCAGCTTTGT
    GCTGTCACAG GGATGGACTG CTCCATTAAA GTCTTTGATC GCTCACTTTT GAAACTCACT
    GGCACCAACA CCTCCAGTCC AAAACATACC AGCAGCGGAG AGGGAAGAAA AACTGTATTG
    CTGAAATCCA GCGAGTGTTC TCCAGAGGTT CTGAACTTTC TGGCTAATAA GGCCTGTGAG
    TGTTATGTGG CCCTGAGCGA TTGGGAGTCT GTGCAAGAAT GGCAGGCCAG CATGATGAAT
    CTCAAAAAGA ACAGCAGCAG TTCCAGTGTC AACCTCAAGA CAGACTTCAA CTACATCAGG
    TCTATGAGCA GGTTTGAGGA GGGAGATTTT ACAGAATGCC GTGCCCAGCT AGAGTTGCTC
    CCTGGGGATG ATTATGGCCT TCTCAACTCT ACAACTAAGG ACAAGATTGA CCTTAAGAGG
    CTTCTTCCTG CTGTGTTGAG TCCAGACCCA TCAGAGCTCC AGAAGGCCAT TGAGGTGCAG
    CTCCTCAGAA GTGCTGTGGG AGCCATCAGT GCGACCAATC ATGAGCAGGA CCAGAAAGTG
    GCATCATCAG ACACCCTGGT TAAATATCTG AAGCAGACGG GTCGCATCTG TCTGGGCCCC
    CTGCGCCTTT CCACTCTCAC CCTGTCAGAT TCCCTGCCCA CCCTTAGCAC TCTACAGCTG
    CACTGTGCCA ACTCTTTAGA GAACTCTCTT TGCAACCAAC ATCCTGAGGA CTGCCTTATT
    CCGCTCTTCA GTGAAGCTTT GACCACCTGC AAGCAGCAGG ATGTTCAACC ATGGCTGCAT
    GCTCTCCGAT ACACTACTTT TCAGCGGGAA TTCTTCCAAA AACTTAAAGG TTCATCTTCT
    CCAGTTGATT CTCATCTGAT GGAGCTTTGT CTGACTGCTG TAAAATTTGC TCGTAAACAA
    GGGAATATTG CTCTGGCCAC ACGCCTGCTG AGTCTATGCA GCAAGCCAGC TATGAGCGAC
    ACTGAAGGTC AGGACCTGGT TCAAAGCTTC AGGCAGCTGT CTCTAGAAGG CACTGTTGGG
    GAAAAGTGGG GACCAGAGCT GGAGATCGAG AAGGCTAAAG TTCTGTTCGC TGCTGGTCAG
    TCTGTGTCTG CTATGGAGAT GTTGAGCTCC TGTGCACTGT CATACTGTCA CTCTGGGAAG
    TGCGAACTTG CGGCCTGTCG CTCGGTGCTC ACTCTGTGCA AATGGCTTTT GGCTGACTGG
    AAAGATCTGA CTCCCCAGCT GAAGATGGTG GTGAAGAAGA ACTCTGGGTC TACCAGCCTC
    TCAACTCTCT CCAAAAACAT CTCTGGCTTG TTGGAGCTGC CGCTGGAAGA TCAGGGAATG
    CCACACATTA CAACAGAGAC TACAGTTAGC GTTGGTGTCG GGGAGCCAGA CTTTGTCCTT
    GGGCAACTTT ACCAGCTCTC AACTACTCAG GCTCCTGAAG TGGCCAAATC TTGGGCTGCT
    CTTGCCAGCT GGGCCTACAG ATGGGGCAGG AAGGTTGTGG ACAATGCCAG TCAAGGAGAA
    GGGGTTCCTC TGCTCCTCGG AGAGAAGAAA GAAATAGAGG AGTTGCTCCC AGCAGGCACC
    AGCGATGAGG ACAAGGAAAC CATTTTTGGC ATTTTGGGTC AAGCCATGTG TCGCCCAGCA
    GGAATTCAGG ATGAGGATAT GGCTCTGCAG AATGAAGAAG ATGATGAGGA CGACATGGTA
    GATGTAATTG GCCGACAGCT GTTGGGTGCC TGTCCCTGGC TTTCAGATGT AGAGGACACT
    GTGACCGATG GACTCATTGG GGTGTGGAGG AGAGTGGTTG ATCGGATCTT CAGCCTCTAC
    CGGGTCTCCT GCAGGGCTTA CTTCACTTTC CTCAAACTCA ATGCTGGACA GGTGCCTATA
    GATGAAGATG ATCCCAAGCT GCTCCTGAAC AACCAGAACA GCAAGCAGAG CAGCGATGAT
    GTCATTGTCA TGGCAACCCT CCGTCTGCTG CGCTTGTTGG TGAAGCACGC AGGAGAGTTG
    AGGGAAGGTC TGGAGCACGG TTTGGCATCC ACACCTACTG CTCCCTGGAG AGGTATTATC
    CCTCAGTTAT TCTCCAGACT TAATCACCCT GAAGCCTACA TCCGCCAGAG CATCTGCAGT
    CTGCTGTGTA GAGTGGCCCA AGATTCCCCA CACCTTATCC TTTATCCTGC CATTGTCGGA
    TCAATCTCTC TTGGTGGAGA GGCTCAGACG GCAGGTAATA AGCTGCCATC GTCCCTGCCA
    ACCCTGCTGG GGAACATGCA GGGAGAGGGT CTTTGTGGAG GTGAGAGCGA GACAGGCAGT
    GGGCCGACCT CTCAGGAGAG CAGCCGCGGG GAAGAGATGG TGATGTATTC CAGCGAGGAT
    CAAGCCATGA TGCAGGACTG CTACAGCAAG ATAGTAGACA AGCTTTCCTC TGCCAACCCT
    ACCATGGTGC TGCAGGTGCA GATGTTGGTG GGCGAGTTGA GGAGGGTCAC ACTGCTCTGG
    GACGAGCTCT GGCTGGGGGT CTTACAGCAG CAGCACATGC ACGTCCTGCG CAGGATTCAA
    CAGCTGGAGG ACGAGGTGAA GAGAGTTCAG AACAACAACA CTCTGCGCAA GGATGAGAAG
    GTGGCTATTA TGAGAGAGAA GCACTCTGCT CTGATGAAGC CTGTGGTTTT TGCGCTGGAT
    CATGTGCGCA GCATTACGGC TGCTCCAGCA GAGACGCCTC ACGAGGAATG GTTTCAGGAG
    ACATATGGAG ATGCCATCCA CAATGCCCTA GAGAGACTGA GGAGCCCTCT AAATCCAGCC
    AATCCTGCCA GCAGCTGGGT GCCCTTTAAA CAGATCATGC TGAGTTTACA GCAGAGGGCA
    CAGAAACGAG CCAGTTACCT TCTGCGTCTG GATGAGATCA GCCCACGTCT GACTGCTATG
    GCTAACACCG AGATGGCTCT GCCGGGAGAG GTGTCTGCTA CAGATGCAGT CACCATCCAG
    AGTGTGGGCA ACACCATCAC CATTCTGCCA ACTAAAACCA AACCCAAGAA GCTCTACTTT
    TTGGGATCGG ATGGGAGAAA CTATCCTTAT CTGTTCAAAG GATTGGAGGA TCTGCATTTG
    GATGAGAGGA TCATGCAGTT CTTGTCTATC GTCAACACCA TGTTCACTAA AGTGAATCAG
    CAGGAGAGCC CGAGGTTTCA AGCCCGTCAT TATTCTGTCA CACCTCTGGG TACCAGATCT
    GGCCTCATCC AGTGGGTGGA TGGAGCCACT CCACTGTTTG GCCTGTACAA ACGTTGGCAG
    CAGCGTGAAG CTGTGGTCCA AGCCCAGAAG GCACAGGACT CCTTCCAGCA GCCTCAGAAT
    CTTCCCATGG TCCCACGGCC CAGTGAGCTT TACTACAGTA AAATCAGTCC TGCCTTAAAG
    GCTGTCGGTC TCAGTTTGGA TGTGTCACGT CGTGATTGGC CGTTAAGTGT TATGAGAGAT
    GTTCTAAGAG AGCTGATGGA AGCCACGCCC CCTAACCTCT TGGCAAAGGA GCTCTGGTGT
    TCCTGCACCA CTCCTAGTGA GTGGTGGAGC GTCACTCAGA CTTACGCCAG ATCCACCGCT
    GTGATGTCAA TGGTGGGATA CATTATTGGC TTGGGGGATC GTCACCTTGA CAACGTGCTG
    ATTGACATGA CGACTGGCGA GGTGGTGCAC ATTGACTATA ACGTCTGTTT TGAGAAGGGA
    AAAAGCTTGA GAGTGCCTGA GAAAGTTCCT TTTCGCATGA CCCACAACAT CGAGACTGCT
    CTGGGGGTCA CAGGAGTTGA GGGAATCTTT AGACTGTCCT GTGAACAGGT GGTCCAGATA
    ATGCGGCGTG GGAGAGAGAC CCTCTTGACT CTTCTAGAGG CCTTTGTCTA TGACCCATTG
    GTGGACTGGA CAGCAGGCGG TGAGGTGGGC TTTGCAGGTG CTGTGTATGG AGGAGGTGGT
    CAGCAGGCTG AGAACAAGCA GAGCAAGAGA GAGATGGAGA GAGACATCAC ACGTAGCCTT
    TTCTCCTCCC GTGTGGCTGA AATTAAGGTG AACTGGTTCA AAAACCGTGA TGAAATGACT
    GGAGTGTTGC CACAGCTGGA GGAGGCAGTG GATGAGTACC TCAACCTGCA GGAGCAGCTC
    ACACAAGTGG AGAAGGTGCA GGGCAAGCTG TTGGAGGAGT TGGAGTTCCT GGAGGGAGCA
    GACACCAGAG CAGACCATCC CATCCACTCC CTGGAGCACA GATACTCAGA ACACACGCAG
    CTGCAGTCCA GACAGAGAAC TGTGCAGGAT GCCATTCAAG GGAAGTTGTC AGATCTGGAT
    CAATGGATCT CTCAGTATCA AGCTGCCTTT GCCAGTTTAG AAGCCACACA GCTTGCCAGC
    CTCCTCCAGG AGATCAGTAG CCCTATTGAT TTGGGTCCGC CCAGTTATGT ACCAGCAACG
    TCGTTCTTGC AGAATGCAGG TCAGGCACAT CTGATCAGCC AGTGTGAAGC GCTGGAGGCT
    GAAGTGAGTG CTCTGTTACA GCAGAGGCGC TCTCAGCTGC GAGGTTGTCT TGAGCACCTG
    CACAGTTATG CCACCGTGGC CCTGCTGTAC CCACGTGCCG TGCTGCACAG ACACAGAGCT
    CACACATGGA AACAGTGGAT GGAGGAGCTG GTGTGTGACA TGACGGTCGA TCACTGCCAG
    ACTATTTATC ACCAATACGA AATGCAGTTT GCACCCCAGC CCCCACCTGC CACGTGTCAG
    TTCCTCTCCA GCATCGAGAT GGCGCTGCAG CACCATGCAG CAGAAACCAA CACTCGTCTG
    CTGCGGCAGG TGGAGCGTCT GAAGGCAGAG GGCGCCAGTG TACCCGTGTG TGAGGAGCAG
    CTGCAGGAGA TCGAGCGCTG CATCAAAGTC TTCCTGCACG AGGACGCTGA GCTTGGCTCC
    TTCAGCCTGG CTGGCATCAT CGTCTCTGCC CTCTGTTCTC TCACCAGGCG TAATTTGGTG
    ATGGAGGGAG CTGCGGCCAG TGCTGGCGAG CAGCTGGTGG AGCTGACGTC TCGTGATGGA
    GCCTGGTTCC TAGAGGAGCT CTGCAGCATG AGTGGAAACA TTACATGTCT TGTGCAGCTG
    TTGCAGCAGT GCCAGCTTCT GTCACATGAC CTGGACATCC CTAGTCCTGC AGAAACTTCG
    CAAGTTGTCT ACCTGACCAA TGGTGTTTAC ACCTGTCTTC AGGAGCTGAA CACAAACTTC
    CGTCAGATCA TCTTCCCAGA GGCTCTGCGC TGCATGCTGA AAGGTGAAAA CACTTTAGAG
    ACCATGTTAG CAGAGCTGGA TGCGCTTATC GATCAGTGTG CAGATGGGGT TTCACTGCAG
    GGGCTTGGAG AGATTTTGAT GGCACACATT CGGAACGCCT CCATGGGTCT GGAAGAGGAT
    CCAGATGACC ACTATCTGGA CGTTACCAGG GTTTTGCGAG CCCAGTACTC TGAGCTGATT
    CAGCCCCGCA GCATGGAGAG CTCAGTGCAG GAAACGCCAA AGATGAGCGC TGGTCAGATG
    TTGCTGGTAG CTTTTGATGG CATGTTTGCC CAGTTGGAGA CAGCTTTTGG ACTTCTCATT
    GACAAGCTGA ACTCTATGGA TGTCCCTGCT GCCTGGAGAA AAGTGGATGT GATCCGGGAG
    TCGAGAGCCA CACAGGCTCA TTTCTTTGAT AATGTCCAGA CCAGGCAGGT CTTGGAGGAG
    ATTTTCTTCT TGAAGAGACT CCAGACCATC CGAGACTTCT TCCGTCTATG TGGTTCATTT
    GCACAGACAC TATCTGGCAC ATGCCCTACA CCCACTGATG ACCCTCCGCC ATCTAATGGC
    CCTGTTCCCA TAGTAAAGCC CTTGTATCGA GGCTCCACGG TGGTGAGCGA GGACCAGATG
    ACGCGGCCCA TCAAAGCCTT TACAGCAGAC TTTGTAAGGC AGATGCTGAT GGGGCTCCCC
    ACCCAGGCTC TGGGTCTAGC CATCTGCAGC TCTCTCAGTG CCCTGGGCAT GGATCTTATT
    GCTCAAGTGG AGGCCAAGGA CTTTGGAGCT GAGGGAAAAG TCTCTCTGGA TGACCTGTGC
    AAGAAAGCTG TAGAGCAGGG GGTGCAGGCA GGACGCCTGT CCCAGCTGCT ACTCAACAGG
    GCCACCGTGC TGGCCAGTTC CTATGACACT GCCTGGAAAA AACTTGACCT GGTCCGACGT
    CTGGAGGTCA GCATTGAAGC CTGTAAGGTC AGCCTGCAGA GGAATCAGCT TCACATCGCC
    ATGTTCCAGT GGCAACATGA GGACATTCTT GGTGCGAGGA CGCAGCCCAT GACAGTCAGT
    CCTCCACCAC GTTCCATTAT CCTCAGCAAC ATGAAAAAGA AGCTCTACAA ACTCAGTCAG
    GATGACGCTT CAATAGGAAG TGTCCAGGAG AAGTTGGCCA GTCTTGAAGG CAGTATAGAG
    CAGAGGCTGA AATGGGCCGG TGGTGCAAAT CCTGCACTCG CTCCAGTGCT GCAGGACTTT
    GAGGCCACTA TAGCAGAACG CCGTGCCTTG GTGATAAAGG AGAGTCAGCG TGCCAACCAG
    GTCACTTTCC TCTGCAGTAC CATTCTCAAC TTTGAAGGTC TGCGCACCCG CACACCCGAA
    GCTTTGAACA TGGATGCTGC CCTTTTTGAG CTTGTGAAAC GATGCCAGGC CACCTGCTCC
    TACGCTGCCC AGTTCAATAC CTCTGTTTCC TCGCTAGAAC TACAGCTGCT GCATAGACTG
    AGTCCTGCCA TGGATATGTC CATTGGTGGT CCTGAGTGGC TGGTGTATGC ACAGAATCAC
    CTGACCCAGG AGATGTCCAG TCAGAGAGCC ATGCAGGAGG AAAGAGAGCA GCAGCTGGAG
    AGCGTGACTG AGACCCTGCA GCTGCTGGTG GACTCCATTA AAGGCACCCT GTCCAATCAC
    AACCGCCAGC TCGCTGACGT TAAACACTTA CTGCGAGCAA TGGCAAAGGA TGAAGAGAAT
    GCCCTTGCAG AAGGAGAGGA AGTGACTTAT GATGGGAGTG TGCGTCAGTT CCTTTCTGAA
    TACAAGGCAT GGCAGGATAA TGTGCAGATA GTGCTGTTCA CAGTAGTGCA GGCTACAGGG
    CAACCACGCA GTCAAGAGCA GATCGAGCTG CTGCAGGAGA TTCCCGCCAC CTTGAAGGAG
    CTTAAGGTTC AGAGCCACAG CATCTACAAT GGATTGGTGG GCTTTGCCTC TCCCCTTGTG
    ACAGAGAGAG GAAGTGACTG TATTAGTCCC ACTTCCACAG TCCAGACCAG CTTTGCCGCT
    GCAGTGCGCT GCAGTGGGGT GAAGACACAG CCTGACAGTA TGTCTCAGAA TGCACGCAAA
    GCTCTTCCTC GAAACTTTGG GACCCCGGCG GACACTCCTC CCAGCACACT GATGATCAAC
    AGCAAAGGAC TGGCCCCTAG CCCCAAAAGA GCCGTGCGAG ATCCCAAAAC TGGGAGAGCT
    GTGCAAGAGA GAAATTCATA TGCTGTTAGT GTGTGGAAAA GGGTGAAGGC CAAGCTGGAG
    GGGAGAGATG TGGATCCTAA CAGGAGAATG AGTGTCACTG AGCAGGTGGA TTATGTCATC
    AAAGAGGCAA CAAACGTGGA CAACCTGGCA CAGTTGTACG AGGGATGGAC GGCATGGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45856
    Clone ID Related Accession (Same CDS sequence) XM_009299453.2 , XM_009299453.3
    Accession Version XM_009299453.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase SMG1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG 
    AATGACTGGC AGCCCAGAAG CGACAGTCTG TCAGCCAGCC AGGACGGTGT GAAGTGCAGT
    GTCTCTAGAG ACCGCGGTTC AGAAGTTCTA TTTGAAATTC TGCCCTCTGA CCGGCCTGGC
    ACCCCACCAC TGCACAATGA CGCCTTCACT GCCACCGATG CACTCGAAGA GGGAGGTGGA
    TACGACGCTG ATGGAGGCCT CTCTGAAAAT GCTTATGGTT GGAAGTCGCT GGGGCAAGAG
    TTGAGAATTA ATGATGTGAC TACTGATATA ACCACTTTTC AGCATGGAAA CCGTGCTTTA
    GCTACAAAGG ACATGAGGAA ATCACAGGAC CGACCGTTGG CTCACTCTGA GGAGTCTCGT
    CTGGCCAATC TTCTGCGGAG AGCATCACGG GAAGATGACC GGGAACGCAG ACTGGCCACT
    ATGAAACAAA TGAAGGAGTT CATTGTGCAC TCTGAAAATA AAGTGGTCCT AGTGAAACAG
    TTGGATTCCA TTTTGAGCAC CCTGAATGAC ATTTTGAATG AGAGTAGTAA GCTCCTGCAG
    GAGCTCAGAC AGGAGGCCGC TTGGTGTCTG GGATTGCTCT GTGCCGCCCT CAGTTATGAA
    GCTGAGAGGA TCTTCAAGTG GATGTTCCTC AAGTTCAGTG TGTCTACAAA GGATGAGGTG
    AAGCTTCTGT ACCTTGTGGC AGTGCACAAA GCACTTGAAA CAGCTGGGGA GAAGAAGGCC
    TTTTCAGCTG TAATGCAGCT GGTGATGAGC AACCTTCAGT CTATATTGGA GAACTTGGAC
    ACACCGGAAC TATTGTGCCA AAGTGTAAAG TGTATTTTAC TGGTGGCCCG TTGTTATCCA
    CACATCTTCA GCACCAACTT CAGAGACACA GTGGATATCT TGGTAGGCTG GCATATAGAT
    CACACACAGA AGCACTCTCT TACTCAACAA GTATCTGGCT GGTTGCAGAG TTTGGAGCAA
    TTTTGGGTTG CAGATTTGGC CTTCTCGACC ACATTATTGG GTCAGTTTTT GGAAGATATG
    GAGGCATATG CTGAGGACCT GAGCCATGTT GTATCTGGAG AATCAGGAGA TGAGGACATT
    CCCCCTCCCA CTGTGTCCTT GCCTAAATTG GCAGCCCTAT TGAGAGTTTT TAGCACTGTT
    GTGCACAGCA TCGGAGAACG GTTTAACCCA ATCCGAGGCC CTCCAATTAC AGAAGCATAT
    GTCACTGATG TTTTGAATCG TGTTTTGGCT TGCGTCACCA CAGCCAAGCA GGTGTTCTTC
    TCTGAGGCGG TGCTGACAGC AGGAAATGAG TGTGTGTGTG TTCTGCTGGT GAGCATAGAC
    TTGGGCGGCC AGCTGATTGA TGCTGTAATC TCATACGGCC TAGACCAGCT GAACTGCTGC
    CAGAACTGCG GACCCGAATA CAGCCTGTCT GTTCTCACCC TGCTCACACT GGTTGTTGAC
    CAAATAAACA CCAAACTTCC GGCTTCTTTT GTGGAAAAGT TGTTGGCCCC AAAATCTCAT
    CTTTTGGAGC TGCGCTTTCA CAGAGAGAGA GAGGTGATGG CGGCAGCACA TGGAGTGTAT
    CATGCTGTGC TAAGCCTGAA GAACATCCCT ATCTTGGAGG CGGCATATAA ACTGGTCTTG
    GGCGAGATGG GCTGTGCACT GAACAGTTTG CTGAGCCCTC TGGGTTTGCC TGATGCCTGT
    CCTCACATCC AACATCCTGC TTTCAGCCAG CTGAACTTTA GCCCAGAGAG AGCTGAGTTT
    GTCCTCATCT TTAACCTCAG TGCTCTCACC ACCATTGGCA ACACCAAGAA CTCCCTCATC
    GGGATGTGGG CTCTGTCTCC TACTGTGTTT GCCCTGCTCA GTCAGAATCT GGTTATTGTT
    CATTCTGATT TGGCAGTTCA TCATCCTGCT GTCCAGTATG CAGTGCTCTA CACTCTCTAC
    TCTCACTGCA CCAGACATGA TCACTTCATC TCCAGCAGCC TGAGCTCCTC GTCCCCATCC
    CTTTTCGACG GTGCTGTCAT CAGCACAGTT ACCACGGCAA CCAAAAGGCA TTTCAGCACG
    CTCCTTAATT TGCTGGGTAT GCTGCTCAGC AAGGATCATC TCAACCCTGA GGCGAGGCGG
    CTGTTACTCA CCTGGTCATT GGAAGTAGCT CTGATGATGA AGAAATCTGA AACTTACGCT
    CCTCTCTTCT CGCTCCCATC CTTCCTCAAA TTCTGCAAAG GGTTACTGGC AAATTCCTTG
    AATGAGGACA CCACCATATG TCTGCAGGCA TGTAACAGTC TACAGGTGCT GTCATCTTCC
    TTGACTATGG AGCTTCTCCA GAGGTGTGTG GACGTGTGCC GAGTTCAGCT GGTTCACTCA
    GCAGTCCGAG TCCGGCAGGC CTTTGGGAAA TTGCTGAGAT CTGTTCCCAT GCACGTCGCA
    CTGAGCAGCG CCCATAGTCA CTCTGAGATT AAGGAGATTT CTCTGGCCAT CCGCCGACAC
    ATGAGCAAAG TGCCTAGCAA CACATTCCAC CCACAGGACT TCTCTGACCT TATTGGGTTT
    ATTCTGTACG GAACGGTCCA TCGTGGGGGG AAGGAGCCCT GGTTGGAGCG TCTGTATCAC
    AGCTGTCAGC GGCTGGAGAA GAAAGACTCT GCTATGGTGC CTCGTGCCCT GCTGAAGACT
    GAGGCAGTGC TGTGGCAGTG GGCGGTGTGG GAAGCTGCTC AGTTTACTGT TCTCTCAAAG
    CTCCGTACAC CTCTGGGAAG AGCCCAGGAT ACCTTCCAGA CTATAGAGGG TATGATCCGT
    AGTCTGGCAG CTCACAGTCT GAACACAGAG CAGGAGCTGA GTCAGTGGAG CGGAGGAGAG
    AGTGATGAGG GTCACCACAC CAACCAGCTC CGACTGGCTC TTCTCCTGCA GTTCCTGGAG
    AACCTGGAGA AACTCATGTA CAACGCCTAT GAAGGCTGTG CCAGTGCTCT CACTGCACCA
    CCAAAGGGCA TTAGAACCTT TTTCTACACA AACAGACAGA CATGCCAGGA CTGGCTAACC
    AGAATTCGTT TGGCCCTAAT GAGGGTTGGC CTTCTCTCCG GTCAGCCAGC GGTCACAGTC
    CGTCATGGCT TTGACCTGCT GACTGAGATT AAAAACAGCA GTACACAGGG TCCAGAAATG
    GAGGTTCCAG TGACCATGCT GGTGGAGGCA TTGTGCGAGT TGAGATGCCC AGAGGCTATT
    CAAGGTCTGG CAGCATGGAG TCTTGCCAAC ACTGGCAAAA GCATGGGCTG GCTGAGCTCT
    GTGGCACTGC AGGCTGAGGG AAAGTTTGAG AAGGCAGCGT TAGAGTACCA AGAGCAGCTT
    TGTGCTGTCA CAGGGATGGA CTGCTCCATT AAAGTCTTTG ATCGCTCACT TTTGAAACTC
    ACTGGCACCA ACACCTCCAG TCCAAAACAT ACCAGCAGCG GAGAGGGAAG AAAAACTGTA
    TTGCTGAAAT CCAGCGAGTG TTCTCCAGAG GTTCTGAACT TTCTGGCTAA TAAGGCCTGT
    GAGTGTTATG TGGCCCTGAG CGATTGGGAG TCTGTGCAAG AATGGCAGGC CAGCATGATG
    AATCTCAAAA AGAACAGCAG CAGTTCCAGT GTCAACCTCA AGACAGACTT CAACTACATC
    AGAGCTATGA GCAGGTTTGA GGAGGGAGAT TTTACAGAAT GCCGTGCCCA GCTAGAGTTG
    CTCCCTGGGG ATGATTATGG CCTTCTCAAC TCTACAACTA AGGACAAGAT TGACCTTAAG
    AGGCTTCTTC CTGCTGTGTT GAGTCCAGAC CCATCAGAGC TCCAGAAGGC CATTGAGGTG
    CAGCTCCTCA GAAGTGCTGT GGGAGCCATC AGTGCGACCA ATCATGAGCA GGACCAGAAA
    GTGGCATCAT CAGACACCCT GGTTAAATAT CTGAAGCAGA CGGGTCGCAT CTGTCTGGGC
    CCCCTGCGCC TTTCCACTCT CACCCTGTCA GATTCCCTGC CCACCCTTAG CACTCTACAG
    CTGCACTGTG CCAACTCTTT AGAGAACTCT CTTTGCAACC AACATCCTGA GGACTGCCTT
    ATTCCGCTCT TCAGTGAAGC TTTGACCACC TGCAAGCAGC AGGATGTTCA ACCATGGCTG
    CATGCTCTCC GATACACTAC TTTTCAGCGG GAATTCTTCC AAAAACTTAA AGGTTCATCT
    TCTCCAGTTG ATTCTCATCT GATGGAGCTT TGTCTGACTG CTGTAAAATT TGCTCGTAAA
    CAAGGGAATA TTGCTCTGGC CACACGCCTG CTGAGTCTAT GCAGCAAGCC AGCTATGAGC
    GACACTGAAG GTCAGGACCT GGTTCAAAGC TTCAGGCAGC TGTCTCTAGA AGGCACTGTT
    GGGGAAAAGT GGGGACCAGA GCTGGAGATC GAGAAGGCTA AAGTTCTGTT CGCTGCTGGT
    CAGTCTGTGT CTGCTATGGA GATGTTGAGC TCCTGTGCAC TGTCATACTG TCACTCTGGG
    AAGTGCGAAC TTGCGGCCTG TCGCTCGGTG CTCACTCTGT GCAAATGGCT TTTGGCTGAC
    TGGAAAGATC TGACTCCCCA GCTGAAGATG GTGGTGAAGA AGAACTCTGG GTCTACCAGC
    CTCTCAACTC TCTCCAAAAA CATCTCTGGC TTGTTGGAGC TGCCGCTGGA AGATCAGGGA
    ATGCCACACA TTACAACAGA GACTACAGTT AGCGTTGGTG TCGGGGAGCC AGACTTTGTC
    CTTGGGCAAC TTTACCAGCT CTCAACTACT CAGGCTCCTG AAGTGGCCAA ATCTTGGGCT
    GCTCTTGCCA GCTGGGCCTA CAGATGGGGC AGGAAGGTTG TGGACAATGC CAGTCAAGGA
    GAAGGGGTTC CTCTGCTCCT CGGAGAGAAG AAAGAAATAG AGGAGTTGCT CCCAGCAGGC
    ACCAGCGATG AGGACAAGGA AACCATTTTT GGCATTTTGG GTCAAGCCAT GTGTCGCCCA
    GCAGGAATTC AGGATGAGGA TATGGCTCTG CAGAATGAAG AAGATGATGA GGACGACATG
    GTAGATGTAA TTGGCCGACA GCTGTTGGGT GCCTGTCCCT GGCTTTCAGA TGTAGAGGAC
    ACTGTGACCG ATGGACTCAT TGGGGTGTGG AGGAGAGTGG TTGATCGGAT CTTCAGCCTC
    TACCGGGTCT CCTGCAGGGC TTACTTCACT TTCCTCAAAC TCAATGCTGG ACAGGTGCCT
    ATAGATGAAG ATGATCCCAA GCTGCTCCTG AACAACCAGA ACAGCAAGCA GAGCAGCGAT
    GATGTCATTG TCATGGCAAC CCTCCGTCTG CTGCGCTTGT TGGTGAAGCA CGCAGGAGAG
    TTGAGGGAAG GTCTGGAGCA CGGTTTGGCA TCCACACCTA CTGCTCCCTG GAGAGGTATT
    ATCCCTCAGT TATTCTCCAG ACTTAATCAC CCTGAAGCCT ACATCCGCCA GAGCATCTGC
    AGTCTGCTGT GTAGAGTGGC CCAAGATTCC CCACACCTTA TCCTTTATCC TGCCATTGTC
    GGATCAATCT CTCTTGGTGG AGAGGCTCAG ACGGCAGGTA ATAAGCTGCC ATCGTCCCTG
    CCAACCCTGC TGGGGAACAT GCAGGGAGAG GGTCTTTGTG GAGGTGAGAG CGAGACAGGC
    AGTGGGCCGA CCTCTCAGGA GAGCAGCCGC GGGGAAGAGA TGGTGATGTA TTCCAGCGAG
    GATCAAGCCA TGATGCAGGA CTGCTACAGC AAGATAGTAG ACAAGCTTTC CTCTGCCAAC
    CCTACCATGG TGCTGCAGGT GCAGATGTTG GTGGGCGAGT TGAGGAGGGT CACACTGCTC
    TGGGACGAGC TCTGGCTGGG GGTCTTACAG CAGCAGCACA TGCACGTCCT GCGCAGGATT
    CAACAGCTGG AGGACGAGGT GAAGAGAGTT CAGAACAACA ACACTCTGCG CAAGGATGAG
    AAGGTGGCTA TTATGAGAGA GAAGCACTCT GCTCTGATGA AGCCTGTGGT TTTTGCGCTG
    GATCATGTGC GCAGCATTAC GGCTGCTCCA GCAGAGACGC CTCACGAGGA ATGGTTTCAG
    GAGACATATG GAGATGCCAT CCACAATGCC CTAGAGAGAC TGAGGAGCCC TCTAAATCCA
    GCCAATCCTG CCAGCAGCTG GGTGCCCTTT AAACAGATCA TGCTGAGTTT ACAGCAGAGG
    GCACAGAAAC GAGCCAGTTA CCTTCTGCGT CTGGATGAGA TCAGCCCACG TCTGACTGCT
    ATGGCTAACA CCGAGATGGC TCTGCCGGGA GAGGTGTCTG CTACAGATGC AGTCACCATC
    CAGAGTGTGG GCAACACCAT CACCATTCTG CCAACTAAAA CCAAACCCAA GAAGCTCTAC
    TTTTTGGGAT CGGATGGGAG AAACTATCCT TATCTGTTCA AAGGATTGGA GGATCTGCAT
    TTGGATGAGA GGATCATGCA GTTCTTGTCT ATCGTCAACA CCATGTTCAC TAAAGTGAAT
    CAGCAGGAGA GCCCGAGGTT TCAAGCCCGT CATTATTCTG TCACACCTCT GGGTACCAGA
    TCTGGCCTCA TCCAGTGGGT GGATGGAGCC ACTCCACTGT TTGGCCTGTA CAAACGTTGG
    CAGCAGCGTG AAGCTGTGGT CCAAGCCCAG AAGGCACAGG ACTCCTTCCA GCAGCCTCAG
    AATCTTCCCA TGGTCCCACG GCCCAGTGAG CTTTACTACA GTAAAATCAG TCCTGCCTTA
    AAGGCTGTCG GTCTCAGTTT GGATGTGTCA CGTCGTGATT GGCCGTTAAG TGTTATGAGA
    GATGTTCTAA GAGAGCTGAT GGAAGCCACG CCCCCTAACC TCTTGGCAAA GGAGCTCTGG
    TGTTCCTGCA CCACTCCTAG TGAGTGGTGG AGCGTCACTC AGACTTACGC CAGATCCACC
    GCTGTGATGT CAATGGTGGG ATACATTATT GGCTTGGGGG ATCGTCACCT TGACAACGTG
    CTGATTGACA TGACGACTGG CGAGGTGGTG CACATTGACT ATAACGTCTG TTTTGAGAAG
    GGAAAAAGCT TGAGAGTGCC TGAGAAAGTT CCTTTTCGCA TGACCCACAA CATCGAGACT
    GCTCTGGGGG TCACAGGAGT TGAGGGAATC TTTAGACTGT CCTGTGAACA GGTGGTCCAG
    ATAATGCGGC GTGGGAGAGA GACCCTCTTG ACTCTTCTAG AGGCCTTTGT CTATGACCCA
    TTGGTGGACT GGACAGCAGG CGGTGAGGTG GGCTTTGCAG GTGCTGTGTA TGGAGGAGGT
    GGTCAGCAGG CTGAGAACAA GCAGAGCAAG AGAGAGATGG AGAGAGACAT CACACGTAGC
    CTTTTCTCCT CCCGTGTGGC TGAAATTAAG GTGAACTGGT TCAAAAACCG TGATGAAATG
    ACTGGAGTGT TGCCACAGCT GGAGGAGGCA GTGGATGAGT ACCTCAACCT GCAGGAGCAG
    CTCACACAAG TGGAGAAGGT GCAGGGCAAG CTGTTGGAGG AGTTGGAGTT CCTGGAGGGA
    GCAGACACCA GAGCAGACCA TCCCATCCAC TCCCTGGAGC ACAGATACTC AGAACACACG
    CAGCTGCAGT CCAGACAGAG AACTGTGCAG GATGCCATTC AAGGGAAGTT GTCAGATCTG
    GATCAATGGA TCTCTCAGTA TCAAGCTGCC TTTGCCAGTT TAGAAGCCAC ACAGCTTGCC
    AGCCTCCTCC AGGAGATCAG TAGCCCTATT GATTTGGGTC CGCCCAGTTA TGTACCAGCA
    ACGTCGTTCT TGCAGAATGC AGGTCAGGCA CATCTGATCA GCCAGTGTGA AGCGCTGGAG
    GCTGAAGTGA GTGCTCTGTT ACAGCAGAGG CGCTCTCAGC TGCGAGGTTG TCTTGAGCAC
    CTGCACAGTT ATGCCACCGT GGCCCTGCTG TACCCACGTG CCGTGCTGCA CAGACACAGA
    GCTCACACAT GGAAACAGTG GATGGAGGAG CTGGTGTGTG ACATGACGGT CGATCACTGC
    CAGACTATTT ATCACCAATA CGAAATGCAG TTTGCACCCC AGCCCCCACC TGCCACGTGT
    CAGTTCCTCT CCAGCATCGA GATGGCGCTG CAGCACCATG CAGCAGAAAC CAACACTCGT
    CTGCTGCGGC AGGTGGAGCG TCTGAAGGCA GAGGGCGCCA GTGTACCCGT GTGTGAGGAG
    CAGCTGCAGG AGATCGAGCG CTGCATCAAA GTCTTCCTGC ACGAGGACGC TGAGCTTGGC
    TCCTTCAGCC TGGCTGGCAT CATCGTCTCT GCCCTCTGTT CTCTCACCAG GCGTAATTTG
    GTGATGGAGG GAGCTGCGGC CAGTGCTGGC GAGCAGCTGG TGGAGCTGAC GTCTCGTGAT
    GGAGCCTGGT TCCTAGAGGA GCTCTGCAGC ATGAGTGGAA ACATTACATG TCTTGTGCAG
    CTGTTGCAGC AGTGCCAGCT TCTGTCACAT GACCTGGACA TCCCTAGTCC TGCAGAAACT
    TCGCAAGTTG TCTACCTGAC CAATGGTGTT TACACCTGTC TTCAGGAGCT GAACACAAAC
    TTCCGTCAGA TCATCTTCCC AGAGGCTCTG CGCTGCATGC TGAAAGGTGA AAACACTTTA
    GAGACCATGT TAGCAGAGCT GGATGCGCTT ATCGATCAGT GTGCAGATGG GGTTTCACTG
    CAGGGGCTTG GAGAGATTTT GATGGCACAC ATTCGGAACG CCTCCATGGG TCTGGAAGAG
    GATCCAGATG ACCACTATCT GGACGTTACC AGGGTTTTGC GAGCCCAGTA CTCTGAGCTG
    ATTCAGCCCC GCAGCATGGA GAGCTCAGTG CAGGAAACGC CAAAGATGAG CGCTGGTCAG
    ATGTTGCTGG TAGCTTTTGA TGGCATGTTT GCCCAGTTGG AGACAGCTTT TGGACTTCTC
    ATTGACAAGC TGAACTCTAT GGATGTCCCT GCTGCCTGGA GAAAAGTGGA TGTGATCCGG
    GAGTCGAGAG CCACACAGGC TCATTTCTTT GATAATGTCC AGACCAGGCA GGTCTTGGAG
    GAGATTTTCT TCTTGAAGAG ACTCCAGACC ATCCGAGACT TCTTCCGTCT ATGTGGTTCA
    TTTGCACAGA CACTATCTGG CACATGCCCT ACACCCACTG ATGACCCTCC GCCATCTAAT
    GGCCCTGTTC CCATAGTAAA GCCCTTGTAT CGAGGCTCCA CGGTGGTGAG CGAGGACCAG
    ATGACGCGGC CCATCAAAGC CTTTACAGCA GACTTTGTAA GGCAGATGCT GATGGGGCTC
    CCCACCCAGG CTCTGGGTCT AGCCATCTGC AGCTCTCTCA GTGCCCTGGG CATGGATCTT
    ATTGCTCAAG TGGAGGCCAA GGACTTTGGA GCTGAGGGAA AAGTCTCTCT GGATGACCTG
    TGCAAGAAAG CTGTAGAGCA GGGGGTGCAG GCAGGACGCC TGTCCCAGCT GCTACTCAAC
    AGGGCCACCG TGCTGGCCAG TTCCTATGAC ACTGCCTGGA AAAAACTTGA CCTGGTCCGA
    CGTCTGGAGG TCAGCATTGA AGCCTGTAAG GTCAGCCTGC AGAGGAATCA GCTTCACATC
    GCCATGTTCC AGTGGCAACA TGAGGACATT CTTGGTGCGA GGACGCAGCC CATGACAGTC
    AGTCCTCCAC CACGTTCCAT TATCCTCAGC AACATGAAAA AGAAGCTCTA CAAACTCAGT
    CAGGATGACG CTTCAATAGG AAGTGTCCAG GAGAAGTTGG CCAGTCTTGA AGGCAGTATA
    GAGCAGAGGC TGAAATGGGC CGGTGGTGCA AATCCTGCAC TCGCTCCAGT GCTGCAGGAC
    TTTGAGGCCA CTATAGCAGA ACGCCGTGCC TTGGTGATAA AGGAGAGTCA GCGTGCCAAC
    CAGGTCACTT TCCTCTGCAG TACCATTCTC AACTTTGAAG GTCTGCGCAC CCGCACACCC
    GAAGCTTTGA ACATGGATGC TGCCCTTTTT GAGCTTGTGA AACGATGCCA GGCCACCTGC
    TCCTACGCTG CCCAGTTCAA TACCTCTGTT TCCTCGCTAG AACTACAGCT GCTGCATAGA
    CTGAGTCCTG CCATGGATAT GTCCATTGGT GGTCCTGAGT GGCTGGTGTA TGCACAGAAT
    CACCTGACCC AGGAGATGTC CAGTCAGAGA GCCATGCAGG AGGAAAGAGA GCAGCAGCTG
    GAGAGCGTGA CTGAGACCCT GCAGCTGCTG GTGGACTCCA TTAAAGGCAC CCTGTCCAAT
    CACAACCGCC AGCTCGCTGA CGTTAAACAC TTACTGCGAG CAATGGCAAA GGATGAAGAG
    AATGCCCTTG CAGAAGGAGA GGAAGTGACT TATGATGGGA GTGTGCGTCA GTTCCTTTCT
    GAATACAAGG CATGGCAGGA TAATGTGCAG ATAGTGCTGT TCACAGTAGT GCAGGCTACA
    GGGCAACCAC GCAGTCAAGA GCAGATCGAG CTGCTGCAGG AGATTCCCGC CACCTTGAAG
    GAGCTTAAGG TTCAGAGCCA CAGCATCTAC AATGGATTGG TGGGCTTTGC CTCTCCCCTT
    GTGACAGAGA GAGGAAGTGA CTGTATTAGT CCCACTTCCA CAGTCCAGAC CAGCTTTGCC
    GCTGCAGTGC GCTGCAGTGG GGTGAAGACA CAGCCTGACA GTATGTCTCA GAATGCACGC
    AAAGCTCTTC CTCGAAACTT TGGGACCCCG GCGGACACTC CTCCCAGCAC ACTGATGATC
    AACAGCAAAG GACTGGCCCC TAGCCCCAAA AGAGCCGTGC GAGATCCCAA AACTGGGAGA
    GCTGTGCAAG AGAGAAATTC ATATGCTGTT AGTGTGTGGA AAAGGGTGAA GGCCAAGCTG
    GAGGGGAGAG ATGTGGATCC TAACAGGAGA ATGAGTGTCA CTGAGCAGGT GGATTATGTC
    ATCAAAGAGG CAACAAACGT GGACAACCTG GCACAGTTGT ACGAGGGATG GACGGCATGG
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297728.1
    CDS175..11100
    Translation

    Target ORF information:

    RefSeq Version XM_009299453.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio SMG1 phosphatidylinositol 3-kinase-related kinase (smg1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299453.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG 
    AATGACTGGC AGCCCAGAAG CGACAGTCTG TCAGCCAGCC AGGACGGTGT GAAGTGCAGT
    GTCTCTAGAG ACCGCGGTTC AGAAGTTCTA TTTGAAATTC TGCCCTCTGA CCGGCCTGGC
    ACCCCACCAC TGCACAATGA CGCCTTCACT GCCACCGATG CACTCGAAGA GGGAGGTGGA
    TACGACGCTG ATGGAGGCCT CTCTGAAAAT GCTTATGGTT GGAAGTCGCT GGGGCAAGAG
    TTGAGAATTA ATGATGTGAC TACTGATATA ACCACTTTTC AGCATGGAAA CCGTGCTTTA
    GCTACAAAGG ACATGAGGAA ATCACAGGAC CGACCGTTGG CTCACTCTGA GGAGTCTCGT
    CTGGCCAATC TTCTGCGGAG AGCATCACGG GAAGATGACC GGGAACGCAG ACTGGCCACT
    ATGAAACAAA TGAAGGAGTT CATTGTGCAC TCTGAAAATA AAGTGGTCCT AGTGAAACAG
    TTGGATTCCA TTTTGAGCAC CCTGAATGAC ATTTTGAATG AGAGTAGTAA GCTCCTGCAG
    GAGCTCAGAC AGGAGGCCGC TTGGTGTCTG GGATTGCTCT GTGCCGCCCT CAGTTATGAA
    GCTGAGAGGA TCTTCAAGTG GATGTTCCTC AAGTTCAGTG TGTCTACAAA GGATGAGGTG
    AAGCTTCTGT ACCTTGTGGC AGTGCACAAA GCACTTGAAA CAGCTGGGGA GAAGAAGGCC
    TTTTCAGCTG TAATGCAGCT GGTGATGAGC AACCTTCAGT CTATATTGGA GAACTTGGAC
    ACACCGGAAC TATTGTGCCA AAGTGTAAAG TGTATTTTAC TGGTGGCCCG TTGTTATCCA
    CACATCTTCA GCACCAACTT CAGAGACACA GTGGATATCT TGGTAGGCTG GCATATAGAT
    CACACACAGA AGCACTCTCT TACTCAACAA GTATCTGGCT GGTTGCAGAG TTTGGAGCAA
    TTTTGGGTTG CAGATTTGGC CTTCTCGACC ACATTATTGG GTCAGTTTTT GGAAGATATG
    GAGGCATATG CTGAGGACCT GAGCCATGTT GTATCTGGAG AATCAGGAGA TGAGGACATT
    CCCCCTCCCA CTGTGTCCTT GCCTAAATTG GCAGCCCTAT TGAGAGTTTT TAGCACTGTT
    GTGCACAGCA TCGGAGAACG GTTTAACCCA ATCCGAGGCC CTCCAATTAC AGAAGCATAT
    GTCACTGATG TTTTGAATCG TGTTTTGGCT TGCGTCACCA CAGCCAAGCA GGTGTTCTTC
    TCTGAGGCGG TGCTGACAGC AGGAAATGAG TGTGTGTGTG TTCTGCTGGT GAGCATAGAC
    TTGGGCGGCC AGCTGATTGA TGCTGTAATC TCATACGGCC TAGACCAGCT GAACTGCTGC
    CAGAACTGCG GACCCGAATA CAGCCTGTCT GTTCTCACCC TGCTCACACT GGTTGTTGAC
    CAAATAAACA CCAAACTTCC GGCTTCTTTT GTGGAAAAGT TGTTGGCCCC AAAATCTCAT
    CTTTTGGAGC TGCGCTTTCA CAGAGAGAGA GAGGTGATGG CGGCAGCACA TGGAGTGTAT
    CATGCTGTGC TAAGCCTGAA GAACATCCCT ATCTTGGAGG CGGCATATAA ACTGGTCTTG
    GGCGAGATGG GCTGTGCACT GAACAGTTTG CTGAGCCCTC TGGGTTTGCC TGATGCCTGT
    CCTCACATCC AACATCCTGC TTTCAGCCAG CTGAACTTTA GCCCAGAGAG AGCTGAGTTT
    GTCCTCATCT TTAACCTCAG TGCTCTCACC ACCATTGGCA ACACCAAGAA CTCCCTCATC
    GGGATGTGGG CTCTGTCTCC TACTGTGTTT GCCCTGCTCA GTCAGAATCT GGTTATTGTT
    CATTCTGATT TGGCAGTTCA TCATCCTGCT GTCCAGTATG CAGTGCTCTA CACTCTCTAC
    TCTCACTGCA CCAGACATGA TCACTTCATC TCCAGCAGCC TGAGCTCCTC GTCCCCATCC
    CTTTTCGACG GTGCTGTCAT CAGCACAGTT ACCACGGCAA CCAAAAGGCA TTTCAGCACG
    CTCCTTAATT TGCTGGGTAT GCTGCTCAGC AAGGATCATC TCAACCCTGA GGCGAGGCGG
    CTGTTACTCA CCTGGTCATT GGAAGTAGCT CTGATGATGA AGAAATCTGA AACTTACGCT
    CCTCTCTTCT CGCTCCCATC CTTCCTCAAA TTCTGCAAAG GGTTACTGGC AAATTCCTTG
    AATGAGGACA CCACCATATG TCTGCAGGCA TGTAACAGTC TACAGGTGCT GTCATCTTCC
    TTGACTATGG AGCTTCTCCA GAGGTGTGTG GACGTGTGCC GAGTTCAGCT GGTTCACTCA
    GCAGTCCGAG TCCGGCAGGC CTTTGGGAAA TTGCTGAGAT CTGTTCCCAT GCACGTCGCA
    CTGAGCAGCG CCCATAGTCA CTCTGAGATT AAGGAGATTT CTCTGGCCAT CCGCCGACAC
    ATGAGCAAAG TGCCTAGCAA CACATTCCAC CCACAGGACT TCTCTGACCT TATTGGGTTT
    ATTCTGTACG GAACGGTCCA TCGTGGGGGG AAGGAGCCCT GGTTGGAGCG TCTGTATCAC
    AGCTGTCAGC GGCTGGAGAA GAAAGACTCT GCTATGGTGC CTCGTGCCCT GCTGAAGACT
    GAGGCAGTGC TGTGGCAGTG GGCGGTGTGG GAAGCTGCTC AGTTTACTGT TCTCTCAAAG
    CTCCGTACAC CTCTGGGAAG AGCCCAGGAT ACCTTCCAGA CTATAGAGGG TATGATCCGT
    AGTCTGGCAG CTCACAGTCT GAACACAGAG CAGGAGCTGA GTCAGTGGAG CGGAGGAGAG
    AGTGATGAGG GTCACCACAC CAACCAGCTC CGACTGGCTC TTCTCCTGCA GTTCCTGGAG
    AACCTGGAGA AACTCATGTA CAACGCCTAT GAAGGCTGTG CCAGTGCTCT CACTGCACCA
    CCAAAGGGCA TTAGAACCTT TTTCTACACA AACAGACAGA CATGCCAGGA CTGGCTAACC
    AGAATTCGTT TGGCCCTAAT GAGGGTTGGC CTTCTCTCCG GTCAGCCAGC GGTCACAGTC
    CGTCATGGCT TTGACCTGCT GACTGAGATT AAAAACAGCA GTACACAGGG TCCAGAAATG
    GAGGTTCCAG TGACCATGCT GGTGGAGGCA TTGTGCGAGT TGAGATGCCC AGAGGCTATT
    CAAGGTCTGG CAGCATGGAG TCTTGCCAAC ACTGGCAAAA GCATGGGCTG GCTGAGCTCT
    GTGGCACTGC AGGCTGAGGG AAAGTTTGAG AAGGCAGCGT TAGAGTACCA AGAGCAGCTT
    TGTGCTGTCA CAGGGATGGA CTGCTCCATT AAAGTCTTTG ATCGCTCACT TTTGAAACTC
    ACTGGCACCA ACACCTCCAG TCCAAAACAT ACCAGCAGCG GAGAGGGAAG AAAAACTGTA
    TTGCTGAAAT CCAGCGAGTG TTCTCCAGAG GTTCTGAACT TTCTGGCTAA TAAGGCCTGT
    GAGTGTTATG TGGCCCTGAG CGATTGGGAG TCTGTGCAAG AATGGCAGGC CAGCATGATG
    AATCTCAAAA AGAACAGCAG CAGTTCCAGT GTCAACCTCA AGACAGACTT CAACTACATC
    AGAGCTATGA GCAGGTTTGA GGAGGGAGAT TTTACAGAAT GCCGTGCCCA GCTAGAGTTG
    CTCCCTGGGG ATGATTATGG CCTTCTCAAC TCTACAACTA AGGACAAGAT TGACCTTAAG
    AGGCTTCTTC CTGCTGTGTT GAGTCCAGAC CCATCAGAGC TCCAGAAGGC CATTGAGGTG
    CAGCTCCTCA GAAGTGCTGT GGGAGCCATC AGTGCGACCA ATCATGAGCA GGACCAGAAA
    GTGGCATCAT CAGACACCCT GGTTAAATAT CTGAAGCAGA CGGGTCGCAT CTGTCTGGGC
    CCCCTGCGCC TTTCCACTCT CACCCTGTCA GATTCCCTGC CCACCCTTAG CACTCTACAG
    CTGCACTGTG CCAACTCTTT AGAGAACTCT CTTTGCAACC AACATCCTGA GGACTGCCTT
    ATTCCGCTCT TCAGTGAAGC TTTGACCACC TGCAAGCAGC AGGATGTTCA ACCATGGCTG
    CATGCTCTCC GATACACTAC TTTTCAGCGG GAATTCTTCC AAAAACTTAA AGGTTCATCT
    TCTCCAGTTG ATTCTCATCT GATGGAGCTT TGTCTGACTG CTGTAAAATT TGCTCGTAAA
    CAAGGGAATA TTGCTCTGGC CACACGCCTG CTGAGTCTAT GCAGCAAGCC AGCTATGAGC
    GACACTGAAG GTCAGGACCT GGTTCAAAGC TTCAGGCAGC TGTCTCTAGA AGGCACTGTT
    GGGGAAAAGT GGGGACCAGA GCTGGAGATC GAGAAGGCTA AAGTTCTGTT CGCTGCTGGT
    CAGTCTGTGT CTGCTATGGA GATGTTGAGC TCCTGTGCAC TGTCATACTG TCACTCTGGG
    AAGTGCGAAC TTGCGGCCTG TCGCTCGGTG CTCACTCTGT GCAAATGGCT TTTGGCTGAC
    TGGAAAGATC TGACTCCCCA GCTGAAGATG GTGGTGAAGA AGAACTCTGG GTCTACCAGC
    CTCTCAACTC TCTCCAAAAA CATCTCTGGC TTGTTGGAGC TGCCGCTGGA AGATCAGGGA
    ATGCCACACA TTACAACAGA GACTACAGTT AGCGTTGGTG TCGGGGAGCC AGACTTTGTC
    CTTGGGCAAC TTTACCAGCT CTCAACTACT CAGGCTCCTG AAGTGGCCAA ATCTTGGGCT
    GCTCTTGCCA GCTGGGCCTA CAGATGGGGC AGGAAGGTTG TGGACAATGC CAGTCAAGGA
    GAAGGGGTTC CTCTGCTCCT CGGAGAGAAG AAAGAAATAG AGGAGTTGCT CCCAGCAGGC
    ACCAGCGATG AGGACAAGGA AACCATTTTT GGCATTTTGG GTCAAGCCAT GTGTCGCCCA
    GCAGGAATTC AGGATGAGGA TATGGCTCTG CAGAATGAAG AAGATGATGA GGACGACATG
    GTAGATGTAA TTGGCCGACA GCTGTTGGGT GCCTGTCCCT GGCTTTCAGA TGTAGAGGAC
    ACTGTGACCG ATGGACTCAT TGGGGTGTGG AGGAGAGTGG TTGATCGGAT CTTCAGCCTC
    TACCGGGTCT CCTGCAGGGC TTACTTCACT TTCCTCAAAC TCAATGCTGG ACAGGTGCCT
    ATAGATGAAG ATGATCCCAA GCTGCTCCTG AACAACCAGA ACAGCAAGCA GAGCAGCGAT
    GATGTCATTG TCATGGCAAC CCTCCGTCTG CTGCGCTTGT TGGTGAAGCA CGCAGGAGAG
    TTGAGGGAAG GTCTGGAGCA CGGTTTGGCA TCCACACCTA CTGCTCCCTG GAGAGGTATT
    ATCCCTCAGT TATTCTCCAG ACTTAATCAC CCTGAAGCCT ACATCCGCCA GAGCATCTGC
    AGTCTGCTGT GTAGAGTGGC CCAAGATTCC CCACACCTTA TCCTTTATCC TGCCATTGTC
    GGATCAATCT CTCTTGGTGG AGAGGCTCAG ACGGCAGGTA ATAAGCTGCC ATCGTCCCTG
    CCAACCCTGC TGGGGAACAT GCAGGGAGAG GGTCTTTGTG GAGGTGAGAG CGAGACAGGC
    AGTGGGCCGA CCTCTCAGGA GAGCAGCCGC GGGGAAGAGA TGGTGATGTA TTCCAGCGAG
    GATCAAGCCA TGATGCAGGA CTGCTACAGC AAGATAGTAG ACAAGCTTTC CTCTGCCAAC
    CCTACCATGG TGCTGCAGGT GCAGATGTTG GTGGGCGAGT TGAGGAGGGT CACACTGCTC
    TGGGACGAGC TCTGGCTGGG GGTCTTACAG CAGCAGCACA TGCACGTCCT GCGCAGGATT
    CAACAGCTGG AGGACGAGGT GAAGAGAGTT CAGAACAACA ACACTCTGCG CAAGGATGAG
    AAGGTGGCTA TTATGAGAGA GAAGCACTCT GCTCTGATGA AGCCTGTGGT TTTTGCGCTG
    GATCATGTGC GCAGCATTAC GGCTGCTCCA GCAGAGACGC CTCACGAGGA ATGGTTTCAG
    GAGACATATG GAGATGCCAT CCACAATGCC CTAGAGAGAC TGAGGAGCCC TCTAAATCCA
    GCCAATCCTG CCAGCAGCTG GGTGCCCTTT AAACAGATCA TGCTGAGTTT ACAGCAGAGG
    GCACAGAAAC GAGCCAGTTA CCTTCTGCGT CTGGATGAGA TCAGCCCACG TCTGACTGCT
    ATGGCTAACA CCGAGATGGC TCTGCCGGGA GAGGTGTCTG CTACAGATGC AGTCACCATC
    CAGAGTGTGG GCAACACCAT CACCATTCTG CCAACTAAAA CCAAACCCAA GAAGCTCTAC
    TTTTTGGGAT CGGATGGGAG AAACTATCCT TATCTGTTCA AAGGATTGGA GGATCTGCAT
    TTGGATGAGA GGATCATGCA GTTCTTGTCT ATCGTCAACA CCATGTTCAC TAAAGTGAAT
    CAGCAGGAGA GCCCGAGGTT TCAAGCCCGT CATTATTCTG TCACACCTCT GGGTACCAGA
    TCTGGCCTCA TCCAGTGGGT GGATGGAGCC ACTCCACTGT TTGGCCTGTA CAAACGTTGG
    CAGCAGCGTG AAGCTGTGGT CCAAGCCCAG AAGGCACAGG ACTCCTTCCA GCAGCCTCAG
    AATCTTCCCA TGGTCCCACG GCCCAGTGAG CTTTACTACA GTAAAATCAG TCCTGCCTTA
    AAGGCTGTCG GTCTCAGTTT GGATGTGTCA CGTCGTGATT GGCCGTTAAG TGTTATGAGA
    GATGTTCTAA GAGAGCTGAT GGAAGCCACG CCCCCTAACC TCTTGGCAAA GGAGCTCTGG
    TGTTCCTGCA CCACTCCTAG TGAGTGGTGG AGCGTCACTC AGACTTACGC CAGATCCACC
    GCTGTGATGT CAATGGTGGG ATACATTATT GGCTTGGGGG ATCGTCACCT TGACAACGTG
    CTGATTGACA TGACGACTGG CGAGGTGGTG CACATTGACT ATAACGTCTG TTTTGAGAAG
    GGAAAAAGCT TGAGAGTGCC TGAGAAAGTT CCTTTTCGCA TGACCCACAA CATCGAGACT
    GCTCTGGGGG TCACAGGAGT TGAGGGAATC TTTAGACTGT CCTGTGAACA GGTGGTCCAG
    ATAATGCGGC GTGGGAGAGA GACCCTCTTG ACTCTTCTAG AGGCCTTTGT CTATGACCCA
    TTGGTGGACT GGACAGCAGG CGGTGAGGTG GGCTTTGCAG GTGCTGTGTA TGGAGGAGGT
    GGTCAGCAGG CTGAGAACAA GCAGAGCAAG AGAGAGATGG AGAGAGACAT CACACGTAGC
    CTTTTCTCCT CCCGTGTGGC TGAAATTAAG GTGAACTGGT TCAAAAACCG TGATGAAATG
    ACTGGAGTGT TGCCACAGCT GGAGGAGGCA GTGGATGAGT ACCTCAACCT GCAGGAGCAG
    CTCACACAAG TGGAGAAGGT GCAGGGCAAG CTGTTGGAGG AGTTGGAGTT CCTGGAGGGA
    GCAGACACCA GAGCAGACCA TCCCATCCAC TCCCTGGAGC ACAGATACTC AGAACACACG
    CAGCTGCAGT CCAGACAGAG AACTGTGCAG GATGCCATTC AAGGGAAGTT GTCAGATCTG
    GATCAATGGA TCTCTCAGTA TCAAGCTGCC TTTGCCAGTT TAGAAGCCAC ACAGCTTGCC
    AGCCTCCTCC AGGAGATCAG TAGCCCTATT GATTTGGGTC CGCCCAGTTA TGTACCAGCA
    ACGTCGTTCT TGCAGAATGC AGGTCAGGCA CATCTGATCA GCCAGTGTGA AGCGCTGGAG
    GCTGAAGTGA GTGCTCTGTT ACAGCAGAGG CGCTCTCAGC TGCGAGGTTG TCTTGAGCAC
    CTGCACAGTT ATGCCACCGT GGCCCTGCTG TACCCACGTG CCGTGCTGCA CAGACACAGA
    GCTCACACAT GGAAACAGTG GATGGAGGAG CTGGTGTGTG ACATGACGGT CGATCACTGC
    CAGACTATTT ATCACCAATA CGAAATGCAG TTTGCACCCC AGCCCCCACC TGCCACGTGT
    CAGTTCCTCT CCAGCATCGA GATGGCGCTG CAGCACCATG CAGCAGAAAC CAACACTCGT
    CTGCTGCGGC AGGTGGAGCG TCTGAAGGCA GAGGGCGCCA GTGTACCCGT GTGTGAGGAG
    CAGCTGCAGG AGATCGAGCG CTGCATCAAA GTCTTCCTGC ACGAGGACGC TGAGCTTGGC
    TCCTTCAGCC TGGCTGGCAT CATCGTCTCT GCCCTCTGTT CTCTCACCAG GCGTAATTTG
    GTGATGGAGG GAGCTGCGGC CAGTGCTGGC GAGCAGCTGG TGGAGCTGAC GTCTCGTGAT
    GGAGCCTGGT TCCTAGAGGA GCTCTGCAGC ATGAGTGGAA ACATTACATG TCTTGTGCAG
    CTGTTGCAGC AGTGCCAGCT TCTGTCACAT GACCTGGACA TCCCTAGTCC TGCAGAAACT
    TCGCAAGTTG TCTACCTGAC CAATGGTGTT TACACCTGTC TTCAGGAGCT GAACACAAAC
    TTCCGTCAGA TCATCTTCCC AGAGGCTCTG CGCTGCATGC TGAAAGGTGA AAACACTTTA
    GAGACCATGT TAGCAGAGCT GGATGCGCTT ATCGATCAGT GTGCAGATGG GGTTTCACTG
    CAGGGGCTTG GAGAGATTTT GATGGCACAC ATTCGGAACG CCTCCATGGG TCTGGAAGAG
    GATCCAGATG ACCACTATCT GGACGTTACC AGGGTTTTGC GAGCCCAGTA CTCTGAGCTG
    ATTCAGCCCC GCAGCATGGA GAGCTCAGTG CAGGAAACGC CAAAGATGAG CGCTGGTCAG
    ATGTTGCTGG TAGCTTTTGA TGGCATGTTT GCCCAGTTGG AGACAGCTTT TGGACTTCTC
    ATTGACAAGC TGAACTCTAT GGATGTCCCT GCTGCCTGGA GAAAAGTGGA TGTGATCCGG
    GAGTCGAGAG CCACACAGGC TCATTTCTTT GATAATGTCC AGACCAGGCA GGTCTTGGAG
    GAGATTTTCT TCTTGAAGAG ACTCCAGACC ATCCGAGACT TCTTCCGTCT ATGTGGTTCA
    TTTGCACAGA CACTATCTGG CACATGCCCT ACACCCACTG ATGACCCTCC GCCATCTAAT
    GGCCCTGTTC CCATAGTAAA GCCCTTGTAT CGAGGCTCCA CGGTGGTGAG CGAGGACCAG
    ATGACGCGGC CCATCAAAGC CTTTACAGCA GACTTTGTAA GGCAGATGCT GATGGGGCTC
    CCCACCCAGG CTCTGGGTCT AGCCATCTGC AGCTCTCTCA GTGCCCTGGG CATGGATCTT
    ATTGCTCAAG TGGAGGCCAA GGACTTTGGA GCTGAGGGAA AAGTCTCTCT GGATGACCTG
    TGCAAGAAAG CTGTAGAGCA GGGGGTGCAG GCAGGACGCC TGTCCCAGCT GCTACTCAAC
    AGGGCCACCG TGCTGGCCAG TTCCTATGAC ACTGCCTGGA AAAAACTTGA CCTGGTCCGA
    CGTCTGGAGG TCAGCATTGA AGCCTGTAAG GTCAGCCTGC AGAGGAATCA GCTTCACATC
    GCCATGTTCC AGTGGCAACA TGAGGACATT CTTGGTGCGA GGACGCAGCC CATGACAGTC
    AGTCCTCCAC CACGTTCCAT TATCCTCAGC AACATGAAAA AGAAGCTCTA CAAACTCAGT
    CAGGATGACG CTTCAATAGG AAGTGTCCAG GAGAAGTTGG CCAGTCTTGA AGGCAGTATA
    GAGCAGAGGC TGAAATGGGC CGGTGGTGCA AATCCTGCAC TCGCTCCAGT GCTGCAGGAC
    TTTGAGGCCA CTATAGCAGA ACGCCGTGCC TTGGTGATAA AGGAGAGTCA GCGTGCCAAC
    CAGGTCACTT TCCTCTGCAG TACCATTCTC AACTTTGAAG GTCTGCGCAC CCGCACACCC
    GAAGCTTTGA ACATGGATGC TGCCCTTTTT GAGCTTGTGA AACGATGCCA GGCCACCTGC
    TCCTACGCTG CCCAGTTCAA TACCTCTGTT TCCTCGCTAG AACTACAGCT GCTGCATAGA
    CTGAGTCCTG CCATGGATAT GTCCATTGGT GGTCCTGAGT GGCTGGTGTA TGCACAGAAT
    CACCTGACCC AGGAGATGTC CAGTCAGAGA GCCATGCAGG AGGAAAGAGA GCAGCAGCTG
    GAGAGCGTGA CTGAGACCCT GCAGCTGCTG GTGGACTCCA TTAAAGGCAC CCTGTCCAAT
    CACAACCGCC AGCTCGCTGA CGTTAAACAC TTACTGCGAG CAATGGCAAA GGATGAAGAG
    AATGCCCTTG CAGAAGGAGA GGAAGTGACT TATGATGGGA GTGTGCGTCA GTTCCTTTCT
    GAATACAAGG CATGGCAGGA TAATGTGCAG ATAGTGCTGT TCACAGTAGT GCAGGCTACA
    GGGCAACCAC GCAGTCAAGA GCAGATCGAG CTGCTGCAGG AGATTCCCGC CACCTTGAAG
    GAGCTTAAGG TTCAGAGCCA CAGCATCTAC AATGGATTGG TGGGCTTTGC CTCTCCCCTT
    GTGACAGAGA GAGGAAGTGA CTGTATTAGT CCCACTTCCA CAGTCCAGAC CAGCTTTGCC
    GCTGCAGTGC GCTGCAGTGG GGTGAAGACA CAGCCTGACA GTATGTCTCA GAATGCACGC
    AAAGCTCTTC CTCGAAACTT TGGGACCCCG GCGGACACTC CTCCCAGCAC ACTGATGATC
    AACAGCAAAG GACTGGCCCC TAGCCCCAAA AGAGCCGTGC GAGATCCCAA AACTGGGAGA
    GCTGTGCAAG AGAGAAATTC ATATGCTGTT AGTGTGTGGA AAAGGGTGAA GGCCAAGCTG
    GAGGGGAGAG ATGTGGATCC TAACAGGAGA ATGAGTGTCA CTGAGCAGGT GGATTATGTC
    ATCAAAGAGG CAACAAACGT GGACAACCTG GCACAGTTGT ACGAGGGATG GACGGCATGG
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45856
    Clone ID Related Accession (Same CDS sequence) XM_009299453.2 , XM_009299453.3
    Accession Version XM_009299453.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase SMG1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299453.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1621
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    1741
    1801
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    1921
    1981
    2041
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    2641
    2701
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    3181
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    4141
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    4261
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    4381
    4441
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    4681
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    4981
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    9061
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    9181
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    9301
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    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG 
    AATGACTGGC AGCCCAGAAG CGACAGTCTG TCAGCCAGCC AGGACGGTGT GAAGTGCAGT
    GTCTCTAGAG ACCGCGGTTC AGAAGTTCTA TTTGAAATTC TGCCCTCTGA CCGGCCTGGC
    ACCCCACCAC TGCACAATGA CGCCTTCACT GCCACCGATG CACTCGAAGA GGGAGGTGGA
    TACGACGCTG ATGGAGGCCT CTCTGAAAAT GCTTATGGTT GGAAGTCGCT GGGGCAAGAG
    TTGAGAATTA ATGATGTGAC TACTGATATA ACCACTTTTC AGCATGGAAA CCGTGCTTTA
    GCTACAAAGG ACATGAGGAA ATCACAGGAC CGACCGTTGG CTCACTCTGA GGAGTCTCGT
    CTGGCCAATC TTCTGCGGAG AGCATCACGG GAAGATGACC GGGAACGCAG ACTGGCCACT
    ATGAAACAAA TGAAGGAGTT CATTGTGCAC TCTGAAAATA AAGTGGTCCT AGTGAAACAG
    TTGGATTCCA TTTTGAGCAC CCTGAATGAC ATTTTGAATG AGAGTAGTAA GCTCCTGCAG
    GAGCTCAGAC AGGAGGCCGC TTGGTGTCTG GGATTGCTCT GTGCCGCCCT CAGTTATGAA
    GCTGAGAGGA TCTTCAAGTG GATGTTCCTC AAGTTCAGTG TGTCTACAAA GGATGAGGTG
    AAGCTTCTGT ACCTTGTGGC AGTGCACAAA GCACTTGAAA CAGCTGGGGA GAAGAAGGCC
    TTTTCAGCTG TAATGCAGCT GGTGATGAGC AACCTTCAGT CTATATTGGA GAACTTGGAC
    ACACCGGAAC TATTGTGCCA AAGTGTAAAG TGTATTTTAC TGGTGGCCCG TTGTTATCCA
    CACATCTTCA GCACCAACTT CAGAGACACA GTGGATATCT TGGTAGGCTG GCATATAGAT
    CACACACAGA AGCACTCTCT TACTCAACAA GTATCTGGCT GGTTGCAGAG TTTGGAGCAA
    TTTTGGGTTG CAGATTTGGC CTTCTCGACC ACATTATTGG GTCAGTTTTT GGAAGATATG
    GAGGCATATG CTGAGGACCT GAGCCATGTT GTATCTGGAG AATCAGGAGA TGAGGACATT
    CCCCCTCCCA CTGTGTCCTT GCCTAAATTG GCAGCCCTAT TGAGAGTTTT TAGCACTGTT
    GTGCACAGCA TCGGAGAACG GTTTAACCCA ATCCGAGGCC CTCCAATTAC AGAAGCATAT
    GTCACTGATG TTTTGAATCG TGTTTTGGCT TGCGTCACCA CAGCCAAGCA GGTGTTCTTC
    TCTGAGGCGG TGCTGACAGC AGGAAATGAG TGTGTGTGTG TTCTGCTGGT GAGCATAGAC
    TTGGGCGGCC AGCTGATTGA TGCTGTAATC TCATACGGCC TAGACCAGCT GAACTGCTGC
    CAGAACTGCG GACCCGAATA CAGCCTGTCT GTTCTCACCC TGCTCACACT GGTTGTTGAC
    CAAATAAACA CCAAACTTCC GGCTTCTTTT GTGGAAAAGT TGTTGGCCCC AAAATCTCAT
    CTTTTGGAGC TGCGCTTTCA CAGAGAGAGA GAGGTGATGG CGGCAGCACA TGGAGTGTAT
    CATGCTGTGC TAAGCCTGAA GAACATCCCT ATCTTGGAGG CGGCATATAA ACTGGTCTTG
    GGCGAGATGG GCTGTGCACT GAACAGTTTG CTGAGCCCTC TGGGTTTGCC TGATGCCTGT
    CCTCACATCC AACATCCTGC TTTCAGCCAG CTGAACTTTA GCCCAGAGAG AGCTGAGTTT
    GTCCTCATCT TTAACCTCAG TGCTCTCACC ACCATTGGCA ACACCAAGAA CTCCCTCATC
    GGGATGTGGG CTCTGTCTCC TACTGTGTTT GCCCTGCTCA GTCAGAATCT GGTTATTGTT
    CATTCTGATT TGGCAGTTCA TCATCCTGCT GTCCAGTATG CAGTGCTCTA CACTCTCTAC
    TCTCACTGCA CCAGACATGA TCACTTCATC TCCAGCAGCC TGAGCTCCTC GTCCCCATCC
    CTTTTCGACG GTGCTGTCAT CAGCACAGTT ACCACGGCAA CCAAAAGGCA TTTCAGCACG
    CTCCTTAATT TGCTGGGTAT GCTGCTCAGC AAGGATCATC TCAACCCTGA GGCGAGGCGG
    CTGTTACTCA CCTGGTCATT GGAAGTAGCT CTGATGATGA AGAAATCTGA AACTTACGCT
    CCTCTCTTCT CGCTCCCATC CTTCCTCAAA TTCTGCAAAG GGTTACTGGC AAATTCCTTG
    AATGAGGACA CCACCATATG TCTGCAGGCA TGTAACAGTC TACAGGTGCT GTCATCTTCC
    TTGACTATGG AGCTTCTCCA GAGGTGTGTG GACGTGTGCC GAGTTCAGCT GGTTCACTCA
    GCAGTCCGAG TCCGGCAGGC CTTTGGGAAA TTGCTGAGAT CTGTTCCCAT GCACGTCGCA
    CTGAGCAGCG CCCATAGTCA CTCTGAGATT AAGGAGATTT CTCTGGCCAT CCGCCGACAC
    ATGAGCAAAG TGCCTAGCAA CACATTCCAC CCACAGGACT TCTCTGACCT TATTGGGTTT
    ATTCTGTACG GAACGGTCCA TCGTGGGGGG AAGGAGCCCT GGTTGGAGCG TCTGTATCAC
    AGCTGTCAGC GGCTGGAGAA GAAAGACTCT GCTATGGTGC CTCGTGCCCT GCTGAAGACT
    GAGGCAGTGC TGTGGCAGTG GGCGGTGTGG GAAGCTGCTC AGTTTACTGT TCTCTCAAAG
    CTCCGTACAC CTCTGGGAAG AGCCCAGGAT ACCTTCCAGA CTATAGAGGG TATGATCCGT
    AGTCTGGCAG CTCACAGTCT GAACACAGAG CAGGAGCTGA GTCAGTGGAG CGGAGGAGAG
    AGTGATGAGG GTCACCACAC CAACCAGCTC CGACTGGCTC TTCTCCTGCA GTTCCTGGAG
    AACCTGGAGA AACTCATGTA CAACGCCTAT GAAGGCTGTG CCAGTGCTCT CACTGCACCA
    CCAAAGGGCA TTAGAACCTT TTTCTACACA AACAGACAGA CATGCCAGGA CTGGCTAACC
    AGAATTCGTT TGGCCCTAAT GAGGGTTGGC CTTCTCTCCG GTCAGCCAGC GGTCACAGTC
    CGTCATGGCT TTGACCTGCT GACTGAGATT AAAAACAGCA GTACACAGGG TCCAGAAATG
    GAGGTTCCAG TGACCATGCT GGTGGAGGCA TTGTGCGAGT TGAGATGCCC AGAGGCTATT
    CAAGGTCTGG CAGCATGGAG TCTTGCCAAC ACTGGCAAAA GCATGGGCTG GCTGAGCTCT
    GTGGCACTGC AGGCTGAGGG AAAGTTTGAG AAGGCAGCGT TAGAGTACCA AGAGCAGCTT
    TGTGCTGTCA CAGGGATGGA CTGCTCCATT AAAGTCTTTG ATCGCTCACT TTTGAAACTC
    ACTGGCACCA ACACCTCCAG TCCAAAACAT ACCAGCAGCG GAGAGGGAAG AAAAACTGTA
    TTGCTGAAAT CCAGCGAGTG TTCTCCAGAG GTTCTGAACT TTCTGGCTAA TAAGGCCTGT
    GAGTGTTATG TGGCCCTGAG CGATTGGGAG TCTGTGCAAG AATGGCAGGC CAGCATGATG
    AATCTCAAAA AGAACAGCAG CAGTTCCAGT GTCAACCTCA AGACAGACTT CAACTACATC
    AGAGCTATGA GCAGGTTTGA GGAGGGAGAT TTTACAGAAT GCCGTGCCCA GCTAGAGTTG
    CTCCCTGGGG ATGATTATGG CCTTCTCAAC TCTACAACTA AGGACAAGAT TGACCTTAAG
    AGGCTTCTTC CTGCTGTGTT GAGTCCAGAC CCATCAGAGC TCCAGAAGGC CATTGAGGTG
    CAGCTCCTCA GAAGTGCTGT GGGAGCCATC AGTGCGACCA ATCATGAGCA GGACCAGAAA
    GTGGCATCAT CAGACACCCT GGTTAAATAT CTGAAGCAGA CGGGTCGCAT CTGTCTGGGC
    CCCCTGCGCC TTTCCACTCT CACCCTGTCA GATTCCCTGC CCACCCTTAG CACTCTACAG
    CTGCACTGTG CCAACTCTTT AGAGAACTCT CTTTGCAACC AACATCCTGA GGACTGCCTT
    ATTCCGCTCT TCAGTGAAGC TTTGACCACC TGCAAGCAGC AGGATGTTCA ACCATGGCTG
    CATGCTCTCC GATACACTAC TTTTCAGCGG GAATTCTTCC AAAAACTTAA AGGTTCATCT
    TCTCCAGTTG ATTCTCATCT GATGGAGCTT TGTCTGACTG CTGTAAAATT TGCTCGTAAA
    CAAGGGAATA TTGCTCTGGC CACACGCCTG CTGAGTCTAT GCAGCAAGCC AGCTATGAGC
    GACACTGAAG GTCAGGACCT GGTTCAAAGC TTCAGGCAGC TGTCTCTAGA AGGCACTGTT
    GGGGAAAAGT GGGGACCAGA GCTGGAGATC GAGAAGGCTA AAGTTCTGTT CGCTGCTGGT
    CAGTCTGTGT CTGCTATGGA GATGTTGAGC TCCTGTGCAC TGTCATACTG TCACTCTGGG
    AAGTGCGAAC TTGCGGCCTG TCGCTCGGTG CTCACTCTGT GCAAATGGCT TTTGGCTGAC
    TGGAAAGATC TGACTCCCCA GCTGAAGATG GTGGTGAAGA AGAACTCTGG GTCTACCAGC
    CTCTCAACTC TCTCCAAAAA CATCTCTGGC TTGTTGGAGC TGCCGCTGGA AGATCAGGGA
    ATGCCACACA TTACAACAGA GACTACAGTT AGCGTTGGTG TCGGGGAGCC AGACTTTGTC
    CTTGGGCAAC TTTACCAGCT CTCAACTACT CAGGCTCCTG AAGTGGCCAA ATCTTGGGCT
    GCTCTTGCCA GCTGGGCCTA CAGATGGGGC AGGAAGGTTG TGGACAATGC CAGTCAAGGA
    GAAGGGGTTC CTCTGCTCCT CGGAGAGAAG AAAGAAATAG AGGAGTTGCT CCCAGCAGGC
    ACCAGCGATG AGGACAAGGA AACCATTTTT GGCATTTTGG GTCAAGCCAT GTGTCGCCCA
    GCAGGAATTC AGGATGAGGA TATGGCTCTG CAGAATGAAG AAGATGATGA GGACGACATG
    GTAGATGTAA TTGGCCGACA GCTGTTGGGT GCCTGTCCCT GGCTTTCAGA TGTAGAGGAC
    ACTGTGACCG ATGGACTCAT TGGGGTGTGG AGGAGAGTGG TTGATCGGAT CTTCAGCCTC
    TACCGGGTCT CCTGCAGGGC TTACTTCACT TTCCTCAAAC TCAATGCTGG ACAGGTGCCT
    ATAGATGAAG ATGATCCCAA GCTGCTCCTG AACAACCAGA ACAGCAAGCA GAGCAGCGAT
    GATGTCATTG TCATGGCAAC CCTCCGTCTG CTGCGCTTGT TGGTGAAGCA CGCAGGAGAG
    TTGAGGGAAG GTCTGGAGCA CGGTTTGGCA TCCACACCTA CTGCTCCCTG GAGAGGTATT
    ATCCCTCAGT TATTCTCCAG ACTTAATCAC CCTGAAGCCT ACATCCGCCA GAGCATCTGC
    AGTCTGCTGT GTAGAGTGGC CCAAGATTCC CCACACCTTA TCCTTTATCC TGCCATTGTC
    GGATCAATCT CTCTTGGTGG AGAGGCTCAG ACGGCAGGTA ATAAGCTGCC ATCGTCCCTG
    CCAACCCTGC TGGGGAACAT GCAGGGAGAG GGTCTTTGTG GAGGTGAGAG CGAGACAGGC
    AGTGGGCCGA CCTCTCAGGA GAGCAGCCGC GGGGAAGAGA TGGTGATGTA TTCCAGCGAG
    GATCAAGCCA TGATGCAGGA CTGCTACAGC AAGATAGTAG ACAAGCTTTC CTCTGCCAAC
    CCTACCATGG TGCTGCAGGT GCAGATGTTG GTGGGCGAGT TGAGGAGGGT CACACTGCTC
    TGGGACGAGC TCTGGCTGGG GGTCTTACAG CAGCAGCACA TGCACGTCCT GCGCAGGATT
    CAACAGCTGG AGGACGAGGT GAAGAGAGTT CAGAACAACA ACACTCTGCG CAAGGATGAG
    AAGGTGGCTA TTATGAGAGA GAAGCACTCT GCTCTGATGA AGCCTGTGGT TTTTGCGCTG
    GATCATGTGC GCAGCATTAC GGCTGCTCCA GCAGAGACGC CTCACGAGGA ATGGTTTCAG
    GAGACATATG GAGATGCCAT CCACAATGCC CTAGAGAGAC TGAGGAGCCC TCTAAATCCA
    GCCAATCCTG CCAGCAGCTG GGTGCCCTTT AAACAGATCA TGCTGAGTTT ACAGCAGAGG
    GCACAGAAAC GAGCCAGTTA CCTTCTGCGT CTGGATGAGA TCAGCCCACG TCTGACTGCT
    ATGGCTAACA CCGAGATGGC TCTGCCGGGA GAGGTGTCTG CTACAGATGC AGTCACCATC
    CAGAGTGTGG GCAACACCAT CACCATTCTG CCAACTAAAA CCAAACCCAA GAAGCTCTAC
    TTTTTGGGAT CGGATGGGAG AAACTATCCT TATCTGTTCA AAGGATTGGA GGATCTGCAT
    TTGGATGAGA GGATCATGCA GTTCTTGTCT ATCGTCAACA CCATGTTCAC TAAAGTGAAT
    CAGCAGGAGA GCCCGAGGTT TCAAGCCCGT CATTATTCTG TCACACCTCT GGGTACCAGA
    TCTGGCCTCA TCCAGTGGGT GGATGGAGCC ACTCCACTGT TTGGCCTGTA CAAACGTTGG
    CAGCAGCGTG AAGCTGTGGT CCAAGCCCAG AAGGCACAGG ACTCCTTCCA GCAGCCTCAG
    AATCTTCCCA TGGTCCCACG GCCCAGTGAG CTTTACTACA GTAAAATCAG TCCTGCCTTA
    AAGGCTGTCG GTCTCAGTTT GGATGTGTCA CGTCGTGATT GGCCGTTAAG TGTTATGAGA
    GATGTTCTAA GAGAGCTGAT GGAAGCCACG CCCCCTAACC TCTTGGCAAA GGAGCTCTGG
    TGTTCCTGCA CCACTCCTAG TGAGTGGTGG AGCGTCACTC AGACTTACGC CAGATCCACC
    GCTGTGATGT CAATGGTGGG ATACATTATT GGCTTGGGGG ATCGTCACCT TGACAACGTG
    CTGATTGACA TGACGACTGG CGAGGTGGTG CACATTGACT ATAACGTCTG TTTTGAGAAG
    GGAAAAAGCT TGAGAGTGCC TGAGAAAGTT CCTTTTCGCA TGACCCACAA CATCGAGACT
    GCTCTGGGGG TCACAGGAGT TGAGGGAATC TTTAGACTGT CCTGTGAACA GGTGGTCCAG
    ATAATGCGGC GTGGGAGAGA GACCCTCTTG ACTCTTCTAG AGGCCTTTGT CTATGACCCA
    TTGGTGGACT GGACAGCAGG CGGTGAGGTG GGCTTTGCAG GTGCTGTGTA TGGAGGAGGT
    GGTCAGCAGG CTGAGAACAA GCAGAGCAAG AGAGAGATGG AGAGAGACAT CACACGTAGC
    CTTTTCTCCT CCCGTGTGGC TGAAATTAAG GTGAACTGGT TCAAAAACCG TGATGAAATG
    ACTGGAGTGT TGCCACAGCT GGAGGAGGCA GTGGATGAGT ACCTCAACCT GCAGGAGCAG
    CTCACACAAG TGGAGAAGGT GCAGGGCAAG CTGTTGGAGG AGTTGGAGTT CCTGGAGGGA
    GCAGACACCA GAGCAGACCA TCCCATCCAC TCCCTGGAGC ACAGATACTC AGAACACACG
    CAGCTGCAGT CCAGACAGAG AACTGTGCAG GATGCCATTC AAGGGAAGTT GTCAGATCTG
    GATCAATGGA TCTCTCAGTA TCAAGCTGCC TTTGCCAGTT TAGAAGCCAC ACAGCTTGCC
    AGCCTCCTCC AGGAGATCAG TAGCCCTATT GATTTGGGTC CGCCCAGTTA TGTACCAGCA
    ACGTCGTTCT TGCAGAATGC AGGTCAGGCA CATCTGATCA GCCAGTGTGA AGCGCTGGAG
    GCTGAAGTGA GTGCTCTGTT ACAGCAGAGG CGCTCTCAGC TGCGAGGTTG TCTTGAGCAC
    CTGCACAGTT ATGCCACCGT GGCCCTGCTG TACCCACGTG CCGTGCTGCA CAGACACAGA
    GCTCACACAT GGAAACAGTG GATGGAGGAG CTGGTGTGTG ACATGACGGT CGATCACTGC
    CAGACTATTT ATCACCAATA CGAAATGCAG TTTGCACCCC AGCCCCCACC TGCCACGTGT
    CAGTTCCTCT CCAGCATCGA GATGGCGCTG CAGCACCATG CAGCAGAAAC CAACACTCGT
    CTGCTGCGGC AGGTGGAGCG TCTGAAGGCA GAGGGCGCCA GTGTACCCGT GTGTGAGGAG
    CAGCTGCAGG AGATCGAGCG CTGCATCAAA GTCTTCCTGC ACGAGGACGC TGAGCTTGGC
    TCCTTCAGCC TGGCTGGCAT CATCGTCTCT GCCCTCTGTT CTCTCACCAG GCGTAATTTG
    GTGATGGAGG GAGCTGCGGC CAGTGCTGGC GAGCAGCTGG TGGAGCTGAC GTCTCGTGAT
    GGAGCCTGGT TCCTAGAGGA GCTCTGCAGC ATGAGTGGAA ACATTACATG TCTTGTGCAG
    CTGTTGCAGC AGTGCCAGCT TCTGTCACAT GACCTGGACA TCCCTAGTCC TGCAGAAACT
    TCGCAAGTTG TCTACCTGAC CAATGGTGTT TACACCTGTC TTCAGGAGCT GAACACAAAC
    TTCCGTCAGA TCATCTTCCC AGAGGCTCTG CGCTGCATGC TGAAAGGTGA AAACACTTTA
    GAGACCATGT TAGCAGAGCT GGATGCGCTT ATCGATCAGT GTGCAGATGG GGTTTCACTG
    CAGGGGCTTG GAGAGATTTT GATGGCACAC ATTCGGAACG CCTCCATGGG TCTGGAAGAG
    GATCCAGATG ACCACTATCT GGACGTTACC AGGGTTTTGC GAGCCCAGTA CTCTGAGCTG
    ATTCAGCCCC GCAGCATGGA GAGCTCAGTG CAGGAAACGC CAAAGATGAG CGCTGGTCAG
    ATGTTGCTGG TAGCTTTTGA TGGCATGTTT GCCCAGTTGG AGACAGCTTT TGGACTTCTC
    ATTGACAAGC TGAACTCTAT GGATGTCCCT GCTGCCTGGA GAAAAGTGGA TGTGATCCGG
    GAGTCGAGAG CCACACAGGC TCATTTCTTT GATAATGTCC AGACCAGGCA GGTCTTGGAG
    GAGATTTTCT TCTTGAAGAG ACTCCAGACC ATCCGAGACT TCTTCCGTCT ATGTGGTTCA
    TTTGCACAGA CACTATCTGG CACATGCCCT ACACCCACTG ATGACCCTCC GCCATCTAAT
    GGCCCTGTTC CCATAGTAAA GCCCTTGTAT CGAGGCTCCA CGGTGGTGAG CGAGGACCAG
    ATGACGCGGC CCATCAAAGC CTTTACAGCA GACTTTGTAA GGCAGATGCT GATGGGGCTC
    CCCACCCAGG CTCTGGGTCT AGCCATCTGC AGCTCTCTCA GTGCCCTGGG CATGGATCTT
    ATTGCTCAAG TGGAGGCCAA GGACTTTGGA GCTGAGGGAA AAGTCTCTCT GGATGACCTG
    TGCAAGAAAG CTGTAGAGCA GGGGGTGCAG GCAGGACGCC TGTCCCAGCT GCTACTCAAC
    AGGGCCACCG TGCTGGCCAG TTCCTATGAC ACTGCCTGGA AAAAACTTGA CCTGGTCCGA
    CGTCTGGAGG TCAGCATTGA AGCCTGTAAG GTCAGCCTGC AGAGGAATCA GCTTCACATC
    GCCATGTTCC AGTGGCAACA TGAGGACATT CTTGGTGCGA GGACGCAGCC CATGACAGTC
    AGTCCTCCAC CACGTTCCAT TATCCTCAGC AACATGAAAA AGAAGCTCTA CAAACTCAGT
    CAGGATGACG CTTCAATAGG AAGTGTCCAG GAGAAGTTGG CCAGTCTTGA AGGCAGTATA
    GAGCAGAGGC TGAAATGGGC CGGTGGTGCA AATCCTGCAC TCGCTCCAGT GCTGCAGGAC
    TTTGAGGCCA CTATAGCAGA ACGCCGTGCC TTGGTGATAA AGGAGAGTCA GCGTGCCAAC
    CAGGTCACTT TCCTCTGCAG TACCATTCTC AACTTTGAAG GTCTGCGCAC CCGCACACCC
    GAAGCTTTGA ACATGGATGC TGCCCTTTTT GAGCTTGTGA AACGATGCCA GGCCACCTGC
    TCCTACGCTG CCCAGTTCAA TACCTCTGTT TCCTCGCTAG AACTACAGCT GCTGCATAGA
    CTGAGTCCTG CCATGGATAT GTCCATTGGT GGTCCTGAGT GGCTGGTGTA TGCACAGAAT
    CACCTGACCC AGGAGATGTC CAGTCAGAGA GCCATGCAGG AGGAAAGAGA GCAGCAGCTG
    GAGAGCGTGA CTGAGACCCT GCAGCTGCTG GTGGACTCCA TTAAAGGCAC CCTGTCCAAT
    CACAACCGCC AGCTCGCTGA CGTTAAACAC TTACTGCGAG CAATGGCAAA GGATGAAGAG
    AATGCCCTTG CAGAAGGAGA GGAAGTGACT TATGATGGGA GTGTGCGTCA GTTCCTTTCT
    GAATACAAGG CATGGCAGGA TAATGTGCAG ATAGTGCTGT TCACAGTAGT GCAGGCTACA
    GGGCAACCAC GCAGTCAAGA GCAGATCGAG CTGCTGCAGG AGATTCCCGC CACCTTGAAG
    GAGCTTAAGG TTCAGAGCCA CAGCATCTAC AATGGATTGG TGGGCTTTGC CTCTCCCCTT
    GTGACAGAGA GAGGAAGTGA CTGTATTAGT CCCACTTCCA CAGTCCAGAC CAGCTTTGCC
    GCTGCAGTGC GCTGCAGTGG GGTGAAGACA CAGCCTGACA GTATGTCTCA GAATGCACGC
    AAAGCTCTTC CTCGAAACTT TGGGACCCCG GCGGACACTC CTCCCAGCAC ACTGATGATC
    AACAGCAAAG GACTGGCCCC TAGCCCCAAA AGAGCCGTGC GAGATCCCAA AACTGGGAGA
    GCTGTGCAAG AGAGAAATTC ATATGCTGTT AGTGTGTGGA AAAGGGTGAA GGCCAAGCTG
    GAGGGGAGAG ATGTGGATCC TAACAGGAGA ATGAGTGTCA CTGAGCAGGT GGATTATGTC
    ATCAAAGAGG CAACAAACGT GGACAACCTG GCACAGTTGT ACGAGGGATG GACGGCATGG
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297728.1
    CDS181..11106
    Translation

    Target ORF information:

    RefSeq Version XM_009299453.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio SMG1 phosphatidylinositol 3-kinase-related kinase (smg1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299453.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG 
    AATGACTGGC AGCCCAGAAG CGACAGTCTG TCAGCCAGCC AGGACGGTGT GAAGTGCAGT
    GTCTCTAGAG ACCGCGGTTC AGAAGTTCTA TTTGAAATTC TGCCCTCTGA CCGGCCTGGC
    ACCCCACCAC TGCACAATGA CGCCTTCACT GCCACCGATG CACTCGAAGA GGGAGGTGGA
    TACGACGCTG ATGGAGGCCT CTCTGAAAAT GCTTATGGTT GGAAGTCGCT GGGGCAAGAG
    TTGAGAATTA ATGATGTGAC TACTGATATA ACCACTTTTC AGCATGGAAA CCGTGCTTTA
    GCTACAAAGG ACATGAGGAA ATCACAGGAC CGACCGTTGG CTCACTCTGA GGAGTCTCGT
    CTGGCCAATC TTCTGCGGAG AGCATCACGG GAAGATGACC GGGAACGCAG ACTGGCCACT
    ATGAAACAAA TGAAGGAGTT CATTGTGCAC TCTGAAAATA AAGTGGTCCT AGTGAAACAG
    TTGGATTCCA TTTTGAGCAC CCTGAATGAC ATTTTGAATG AGAGTAGTAA GCTCCTGCAG
    GAGCTCAGAC AGGAGGCCGC TTGGTGTCTG GGATTGCTCT GTGCCGCCCT CAGTTATGAA
    GCTGAGAGGA TCTTCAAGTG GATGTTCCTC AAGTTCAGTG TGTCTACAAA GGATGAGGTG
    AAGCTTCTGT ACCTTGTGGC AGTGCACAAA GCACTTGAAA CAGCTGGGGA GAAGAAGGCC
    TTTTCAGCTG TAATGCAGCT GGTGATGAGC AACCTTCAGT CTATATTGGA GAACTTGGAC
    ACACCGGAAC TATTGTGCCA AAGTGTAAAG TGTATTTTAC TGGTGGCCCG TTGTTATCCA
    CACATCTTCA GCACCAACTT CAGAGACACA GTGGATATCT TGGTAGGCTG GCATATAGAT
    CACACACAGA AGCACTCTCT TACTCAACAA GTATCTGGCT GGTTGCAGAG TTTGGAGCAA
    TTTTGGGTTG CAGATTTGGC CTTCTCGACC ACATTATTGG GTCAGTTTTT GGAAGATATG
    GAGGCATATG CTGAGGACCT GAGCCATGTT GTATCTGGAG AATCAGGAGA TGAGGACATT
    CCCCCTCCCA CTGTGTCCTT GCCTAAATTG GCAGCCCTAT TGAGAGTTTT TAGCACTGTT
    GTGCACAGCA TCGGAGAACG GTTTAACCCA ATCCGAGGCC CTCCAATTAC AGAAGCATAT
    GTCACTGATG TTTTGAATCG TGTTTTGGCT TGCGTCACCA CAGCCAAGCA GGTGTTCTTC
    TCTGAGGCGG TGCTGACAGC AGGAAATGAG TGTGTGTGTG TTCTGCTGGT GAGCATAGAC
    TTGGGCGGCC AGCTGATTGA TGCTGTAATC TCATACGGCC TAGACCAGCT GAACTGCTGC
    CAGAACTGCG GACCCGAATA CAGCCTGTCT GTTCTCACCC TGCTCACACT GGTTGTTGAC
    CAAATAAACA CCAAACTTCC GGCTTCTTTT GTGGAAAAGT TGTTGGCCCC AAAATCTCAT
    CTTTTGGAGC TGCGCTTTCA CAGAGAGAGA GAGGTGATGG CGGCAGCACA TGGAGTGTAT
    CATGCTGTGC TAAGCCTGAA GAACATCCCT ATCTTGGAGG CGGCATATAA ACTGGTCTTG
    GGCGAGATGG GCTGTGCACT GAACAGTTTG CTGAGCCCTC TGGGTTTGCC TGATGCCTGT
    CCTCACATCC AACATCCTGC TTTCAGCCAG CTGAACTTTA GCCCAGAGAG AGCTGAGTTT
    GTCCTCATCT TTAACCTCAG TGCTCTCACC ACCATTGGCA ACACCAAGAA CTCCCTCATC
    GGGATGTGGG CTCTGTCTCC TACTGTGTTT GCCCTGCTCA GTCAGAATCT GGTTATTGTT
    CATTCTGATT TGGCAGTTCA TCATCCTGCT GTCCAGTATG CAGTGCTCTA CACTCTCTAC
    TCTCACTGCA CCAGACATGA TCACTTCATC TCCAGCAGCC TGAGCTCCTC GTCCCCATCC
    CTTTTCGACG GTGCTGTCAT CAGCACAGTT ACCACGGCAA CCAAAAGGCA TTTCAGCACG
    CTCCTTAATT TGCTGGGTAT GCTGCTCAGC AAGGATCATC TCAACCCTGA GGCGAGGCGG
    CTGTTACTCA CCTGGTCATT GGAAGTAGCT CTGATGATGA AGAAATCTGA AACTTACGCT
    CCTCTCTTCT CGCTCCCATC CTTCCTCAAA TTCTGCAAAG GGTTACTGGC AAATTCCTTG
    AATGAGGACA CCACCATATG TCTGCAGGCA TGTAACAGTC TACAGGTGCT GTCATCTTCC
    TTGACTATGG AGCTTCTCCA GAGGTGTGTG GACGTGTGCC GAGTTCAGCT GGTTCACTCA
    GCAGTCCGAG TCCGGCAGGC CTTTGGGAAA TTGCTGAGAT CTGTTCCCAT GCACGTCGCA
    CTGAGCAGCG CCCATAGTCA CTCTGAGATT AAGGAGATTT CTCTGGCCAT CCGCCGACAC
    ATGAGCAAAG TGCCTAGCAA CACATTCCAC CCACAGGACT TCTCTGACCT TATTGGGTTT
    ATTCTGTACG GAACGGTCCA TCGTGGGGGG AAGGAGCCCT GGTTGGAGCG TCTGTATCAC
    AGCTGTCAGC GGCTGGAGAA GAAAGACTCT GCTATGGTGC CTCGTGCCCT GCTGAAGACT
    GAGGCAGTGC TGTGGCAGTG GGCGGTGTGG GAAGCTGCTC AGTTTACTGT TCTCTCAAAG
    CTCCGTACAC CTCTGGGAAG AGCCCAGGAT ACCTTCCAGA CTATAGAGGG TATGATCCGT
    AGTCTGGCAG CTCACAGTCT GAACACAGAG CAGGAGCTGA GTCAGTGGAG CGGAGGAGAG
    AGTGATGAGG GTCACCACAC CAACCAGCTC CGACTGGCTC TTCTCCTGCA GTTCCTGGAG
    AACCTGGAGA AACTCATGTA CAACGCCTAT GAAGGCTGTG CCAGTGCTCT CACTGCACCA
    CCAAAGGGCA TTAGAACCTT TTTCTACACA AACAGACAGA CATGCCAGGA CTGGCTAACC
    AGAATTCGTT TGGCCCTAAT GAGGGTTGGC CTTCTCTCCG GTCAGCCAGC GGTCACAGTC
    CGTCATGGCT TTGACCTGCT GACTGAGATT AAAAACAGCA GTACACAGGG TCCAGAAATG
    GAGGTTCCAG TGACCATGCT GGTGGAGGCA TTGTGCGAGT TGAGATGCCC AGAGGCTATT
    CAAGGTCTGG CAGCATGGAG TCTTGCCAAC ACTGGCAAAA GCATGGGCTG GCTGAGCTCT
    GTGGCACTGC AGGCTGAGGG AAAGTTTGAG AAGGCAGCGT TAGAGTACCA AGAGCAGCTT
    TGTGCTGTCA CAGGGATGGA CTGCTCCATT AAAGTCTTTG ATCGCTCACT TTTGAAACTC
    ACTGGCACCA ACACCTCCAG TCCAAAACAT ACCAGCAGCG GAGAGGGAAG AAAAACTGTA
    TTGCTGAAAT CCAGCGAGTG TTCTCCAGAG GTTCTGAACT TTCTGGCTAA TAAGGCCTGT
    GAGTGTTATG TGGCCCTGAG CGATTGGGAG TCTGTGCAAG AATGGCAGGC CAGCATGATG
    AATCTCAAAA AGAACAGCAG CAGTTCCAGT GTCAACCTCA AGACAGACTT CAACTACATC
    AGAGCTATGA GCAGGTTTGA GGAGGGAGAT TTTACAGAAT GCCGTGCCCA GCTAGAGTTG
    CTCCCTGGGG ATGATTATGG CCTTCTCAAC TCTACAACTA AGGACAAGAT TGACCTTAAG
    AGGCTTCTTC CTGCTGTGTT GAGTCCAGAC CCATCAGAGC TCCAGAAGGC CATTGAGGTG
    CAGCTCCTCA GAAGTGCTGT GGGAGCCATC AGTGCGACCA ATCATGAGCA GGACCAGAAA
    GTGGCATCAT CAGACACCCT GGTTAAATAT CTGAAGCAGA CGGGTCGCAT CTGTCTGGGC
    CCCCTGCGCC TTTCCACTCT CACCCTGTCA GATTCCCTGC CCACCCTTAG CACTCTACAG
    CTGCACTGTG CCAACTCTTT AGAGAACTCT CTTTGCAACC AACATCCTGA GGACTGCCTT
    ATTCCGCTCT TCAGTGAAGC TTTGACCACC TGCAAGCAGC AGGATGTTCA ACCATGGCTG
    CATGCTCTCC GATACACTAC TTTTCAGCGG GAATTCTTCC AAAAACTTAA AGGTTCATCT
    TCTCCAGTTG ATTCTCATCT GATGGAGCTT TGTCTGACTG CTGTAAAATT TGCTCGTAAA
    CAAGGGAATA TTGCTCTGGC CACACGCCTG CTGAGTCTAT GCAGCAAGCC AGCTATGAGC
    GACACTGAAG GTCAGGACCT GGTTCAAAGC TTCAGGCAGC TGTCTCTAGA AGGCACTGTT
    GGGGAAAAGT GGGGACCAGA GCTGGAGATC GAGAAGGCTA AAGTTCTGTT CGCTGCTGGT
    CAGTCTGTGT CTGCTATGGA GATGTTGAGC TCCTGTGCAC TGTCATACTG TCACTCTGGG
    AAGTGCGAAC TTGCGGCCTG TCGCTCGGTG CTCACTCTGT GCAAATGGCT TTTGGCTGAC
    TGGAAAGATC TGACTCCCCA GCTGAAGATG GTGGTGAAGA AGAACTCTGG GTCTACCAGC
    CTCTCAACTC TCTCCAAAAA CATCTCTGGC TTGTTGGAGC TGCCGCTGGA AGATCAGGGA
    ATGCCACACA TTACAACAGA GACTACAGTT AGCGTTGGTG TCGGGGAGCC AGACTTTGTC
    CTTGGGCAAC TTTACCAGCT CTCAACTACT CAGGCTCCTG AAGTGGCCAA ATCTTGGGCT
    GCTCTTGCCA GCTGGGCCTA CAGATGGGGC AGGAAGGTTG TGGACAATGC CAGTCAAGGA
    GAAGGGGTTC CTCTGCTCCT CGGAGAGAAG AAAGAAATAG AGGAGTTGCT CCCAGCAGGC
    ACCAGCGATG AGGACAAGGA AACCATTTTT GGCATTTTGG GTCAAGCCAT GTGTCGCCCA
    GCAGGAATTC AGGATGAGGA TATGGCTCTG CAGAATGAAG AAGATGATGA GGACGACATG
    GTAGATGTAA TTGGCCGACA GCTGTTGGGT GCCTGTCCCT GGCTTTCAGA TGTAGAGGAC
    ACTGTGACCG ATGGACTCAT TGGGGTGTGG AGGAGAGTGG TTGATCGGAT CTTCAGCCTC
    TACCGGGTCT CCTGCAGGGC TTACTTCACT TTCCTCAAAC TCAATGCTGG ACAGGTGCCT
    ATAGATGAAG ATGATCCCAA GCTGCTCCTG AACAACCAGA ACAGCAAGCA GAGCAGCGAT
    GATGTCATTG TCATGGCAAC CCTCCGTCTG CTGCGCTTGT TGGTGAAGCA CGCAGGAGAG
    TTGAGGGAAG GTCTGGAGCA CGGTTTGGCA TCCACACCTA CTGCTCCCTG GAGAGGTATT
    ATCCCTCAGT TATTCTCCAG ACTTAATCAC CCTGAAGCCT ACATCCGCCA GAGCATCTGC
    AGTCTGCTGT GTAGAGTGGC CCAAGATTCC CCACACCTTA TCCTTTATCC TGCCATTGTC
    GGATCAATCT CTCTTGGTGG AGAGGCTCAG ACGGCAGGTA ATAAGCTGCC ATCGTCCCTG
    CCAACCCTGC TGGGGAACAT GCAGGGAGAG GGTCTTTGTG GAGGTGAGAG CGAGACAGGC
    AGTGGGCCGA CCTCTCAGGA GAGCAGCCGC GGGGAAGAGA TGGTGATGTA TTCCAGCGAG
    GATCAAGCCA TGATGCAGGA CTGCTACAGC AAGATAGTAG ACAAGCTTTC CTCTGCCAAC
    CCTACCATGG TGCTGCAGGT GCAGATGTTG GTGGGCGAGT TGAGGAGGGT CACACTGCTC
    TGGGACGAGC TCTGGCTGGG GGTCTTACAG CAGCAGCACA TGCACGTCCT GCGCAGGATT
    CAACAGCTGG AGGACGAGGT GAAGAGAGTT CAGAACAACA ACACTCTGCG CAAGGATGAG
    AAGGTGGCTA TTATGAGAGA GAAGCACTCT GCTCTGATGA AGCCTGTGGT TTTTGCGCTG
    GATCATGTGC GCAGCATTAC GGCTGCTCCA GCAGAGACGC CTCACGAGGA ATGGTTTCAG
    GAGACATATG GAGATGCCAT CCACAATGCC CTAGAGAGAC TGAGGAGCCC TCTAAATCCA
    GCCAATCCTG CCAGCAGCTG GGTGCCCTTT AAACAGATCA TGCTGAGTTT ACAGCAGAGG
    GCACAGAAAC GAGCCAGTTA CCTTCTGCGT CTGGATGAGA TCAGCCCACG TCTGACTGCT
    ATGGCTAACA CCGAGATGGC TCTGCCGGGA GAGGTGTCTG CTACAGATGC AGTCACCATC
    CAGAGTGTGG GCAACACCAT CACCATTCTG CCAACTAAAA CCAAACCCAA GAAGCTCTAC
    TTTTTGGGAT CGGATGGGAG AAACTATCCT TATCTGTTCA AAGGATTGGA GGATCTGCAT
    TTGGATGAGA GGATCATGCA GTTCTTGTCT ATCGTCAACA CCATGTTCAC TAAAGTGAAT
    CAGCAGGAGA GCCCGAGGTT TCAAGCCCGT CATTATTCTG TCACACCTCT GGGTACCAGA
    TCTGGCCTCA TCCAGTGGGT GGATGGAGCC ACTCCACTGT TTGGCCTGTA CAAACGTTGG
    CAGCAGCGTG AAGCTGTGGT CCAAGCCCAG AAGGCACAGG ACTCCTTCCA GCAGCCTCAG
    AATCTTCCCA TGGTCCCACG GCCCAGTGAG CTTTACTACA GTAAAATCAG TCCTGCCTTA
    AAGGCTGTCG GTCTCAGTTT GGATGTGTCA CGTCGTGATT GGCCGTTAAG TGTTATGAGA
    GATGTTCTAA GAGAGCTGAT GGAAGCCACG CCCCCTAACC TCTTGGCAAA GGAGCTCTGG
    TGTTCCTGCA CCACTCCTAG TGAGTGGTGG AGCGTCACTC AGACTTACGC CAGATCCACC
    GCTGTGATGT CAATGGTGGG ATACATTATT GGCTTGGGGG ATCGTCACCT TGACAACGTG
    CTGATTGACA TGACGACTGG CGAGGTGGTG CACATTGACT ATAACGTCTG TTTTGAGAAG
    GGAAAAAGCT TGAGAGTGCC TGAGAAAGTT CCTTTTCGCA TGACCCACAA CATCGAGACT
    GCTCTGGGGG TCACAGGAGT TGAGGGAATC TTTAGACTGT CCTGTGAACA GGTGGTCCAG
    ATAATGCGGC GTGGGAGAGA GACCCTCTTG ACTCTTCTAG AGGCCTTTGT CTATGACCCA
    TTGGTGGACT GGACAGCAGG CGGTGAGGTG GGCTTTGCAG GTGCTGTGTA TGGAGGAGGT
    GGTCAGCAGG CTGAGAACAA GCAGAGCAAG AGAGAGATGG AGAGAGACAT CACACGTAGC
    CTTTTCTCCT CCCGTGTGGC TGAAATTAAG GTGAACTGGT TCAAAAACCG TGATGAAATG
    ACTGGAGTGT TGCCACAGCT GGAGGAGGCA GTGGATGAGT ACCTCAACCT GCAGGAGCAG
    CTCACACAAG TGGAGAAGGT GCAGGGCAAG CTGTTGGAGG AGTTGGAGTT CCTGGAGGGA
    GCAGACACCA GAGCAGACCA TCCCATCCAC TCCCTGGAGC ACAGATACTC AGAACACACG
    CAGCTGCAGT CCAGACAGAG AACTGTGCAG GATGCCATTC AAGGGAAGTT GTCAGATCTG
    GATCAATGGA TCTCTCAGTA TCAAGCTGCC TTTGCCAGTT TAGAAGCCAC ACAGCTTGCC
    AGCCTCCTCC AGGAGATCAG TAGCCCTATT GATTTGGGTC CGCCCAGTTA TGTACCAGCA
    ACGTCGTTCT TGCAGAATGC AGGTCAGGCA CATCTGATCA GCCAGTGTGA AGCGCTGGAG
    GCTGAAGTGA GTGCTCTGTT ACAGCAGAGG CGCTCTCAGC TGCGAGGTTG TCTTGAGCAC
    CTGCACAGTT ATGCCACCGT GGCCCTGCTG TACCCACGTG CCGTGCTGCA CAGACACAGA
    GCTCACACAT GGAAACAGTG GATGGAGGAG CTGGTGTGTG ACATGACGGT CGATCACTGC
    CAGACTATTT ATCACCAATA CGAAATGCAG TTTGCACCCC AGCCCCCACC TGCCACGTGT
    CAGTTCCTCT CCAGCATCGA GATGGCGCTG CAGCACCATG CAGCAGAAAC CAACACTCGT
    CTGCTGCGGC AGGTGGAGCG TCTGAAGGCA GAGGGCGCCA GTGTACCCGT GTGTGAGGAG
    CAGCTGCAGG AGATCGAGCG CTGCATCAAA GTCTTCCTGC ACGAGGACGC TGAGCTTGGC
    TCCTTCAGCC TGGCTGGCAT CATCGTCTCT GCCCTCTGTT CTCTCACCAG GCGTAATTTG
    GTGATGGAGG GAGCTGCGGC CAGTGCTGGC GAGCAGCTGG TGGAGCTGAC GTCTCGTGAT
    GGAGCCTGGT TCCTAGAGGA GCTCTGCAGC ATGAGTGGAA ACATTACATG TCTTGTGCAG
    CTGTTGCAGC AGTGCCAGCT TCTGTCACAT GACCTGGACA TCCCTAGTCC TGCAGAAACT
    TCGCAAGTTG TCTACCTGAC CAATGGTGTT TACACCTGTC TTCAGGAGCT GAACACAAAC
    TTCCGTCAGA TCATCTTCCC AGAGGCTCTG CGCTGCATGC TGAAAGGTGA AAACACTTTA
    GAGACCATGT TAGCAGAGCT GGATGCGCTT ATCGATCAGT GTGCAGATGG GGTTTCACTG
    CAGGGGCTTG GAGAGATTTT GATGGCACAC ATTCGGAACG CCTCCATGGG TCTGGAAGAG
    GATCCAGATG ACCACTATCT GGACGTTACC AGGGTTTTGC GAGCCCAGTA CTCTGAGCTG
    ATTCAGCCCC GCAGCATGGA GAGCTCAGTG CAGGAAACGC CAAAGATGAG CGCTGGTCAG
    ATGTTGCTGG TAGCTTTTGA TGGCATGTTT GCCCAGTTGG AGACAGCTTT TGGACTTCTC
    ATTGACAAGC TGAACTCTAT GGATGTCCCT GCTGCCTGGA GAAAAGTGGA TGTGATCCGG
    GAGTCGAGAG CCACACAGGC TCATTTCTTT GATAATGTCC AGACCAGGCA GGTCTTGGAG
    GAGATTTTCT TCTTGAAGAG ACTCCAGACC ATCCGAGACT TCTTCCGTCT ATGTGGTTCA
    TTTGCACAGA CACTATCTGG CACATGCCCT ACACCCACTG ATGACCCTCC GCCATCTAAT
    GGCCCTGTTC CCATAGTAAA GCCCTTGTAT CGAGGCTCCA CGGTGGTGAG CGAGGACCAG
    ATGACGCGGC CCATCAAAGC CTTTACAGCA GACTTTGTAA GGCAGATGCT GATGGGGCTC
    CCCACCCAGG CTCTGGGTCT AGCCATCTGC AGCTCTCTCA GTGCCCTGGG CATGGATCTT
    ATTGCTCAAG TGGAGGCCAA GGACTTTGGA GCTGAGGGAA AAGTCTCTCT GGATGACCTG
    TGCAAGAAAG CTGTAGAGCA GGGGGTGCAG GCAGGACGCC TGTCCCAGCT GCTACTCAAC
    AGGGCCACCG TGCTGGCCAG TTCCTATGAC ACTGCCTGGA AAAAACTTGA CCTGGTCCGA
    CGTCTGGAGG TCAGCATTGA AGCCTGTAAG GTCAGCCTGC AGAGGAATCA GCTTCACATC
    GCCATGTTCC AGTGGCAACA TGAGGACATT CTTGGTGCGA GGACGCAGCC CATGACAGTC
    AGTCCTCCAC CACGTTCCAT TATCCTCAGC AACATGAAAA AGAAGCTCTA CAAACTCAGT
    CAGGATGACG CTTCAATAGG AAGTGTCCAG GAGAAGTTGG CCAGTCTTGA AGGCAGTATA
    GAGCAGAGGC TGAAATGGGC CGGTGGTGCA AATCCTGCAC TCGCTCCAGT GCTGCAGGAC
    TTTGAGGCCA CTATAGCAGA ACGCCGTGCC TTGGTGATAA AGGAGAGTCA GCGTGCCAAC
    CAGGTCACTT TCCTCTGCAG TACCATTCTC AACTTTGAAG GTCTGCGCAC CCGCACACCC
    GAAGCTTTGA ACATGGATGC TGCCCTTTTT GAGCTTGTGA AACGATGCCA GGCCACCTGC
    TCCTACGCTG CCCAGTTCAA TACCTCTGTT TCCTCGCTAG AACTACAGCT GCTGCATAGA
    CTGAGTCCTG CCATGGATAT GTCCATTGGT GGTCCTGAGT GGCTGGTGTA TGCACAGAAT
    CACCTGACCC AGGAGATGTC CAGTCAGAGA GCCATGCAGG AGGAAAGAGA GCAGCAGCTG
    GAGAGCGTGA CTGAGACCCT GCAGCTGCTG GTGGACTCCA TTAAAGGCAC CCTGTCCAAT
    CACAACCGCC AGCTCGCTGA CGTTAAACAC TTACTGCGAG CAATGGCAAA GGATGAAGAG
    AATGCCCTTG CAGAAGGAGA GGAAGTGACT TATGATGGGA GTGTGCGTCA GTTCCTTTCT
    GAATACAAGG CATGGCAGGA TAATGTGCAG ATAGTGCTGT TCACAGTAGT GCAGGCTACA
    GGGCAACCAC GCAGTCAAGA GCAGATCGAG CTGCTGCAGG AGATTCCCGC CACCTTGAAG
    GAGCTTAAGG TTCAGAGCCA CAGCATCTAC AATGGATTGG TGGGCTTTGC CTCTCCCCTT
    GTGACAGAGA GAGGAAGTGA CTGTATTAGT CCCACTTCCA CAGTCCAGAC CAGCTTTGCC
    GCTGCAGTGC GCTGCAGTGG GGTGAAGACA CAGCCTGACA GTATGTCTCA GAATGCACGC
    AAAGCTCTTC CTCGAAACTT TGGGACCCCG GCGGACACTC CTCCCAGCAC ACTGATGATC
    AACAGCAAAG GACTGGCCCC TAGCCCCAAA AGAGCCGTGC GAGATCCCAA AACTGGGAGA
    GCTGTGCAAG AGAGAAATTC ATATGCTGTT AGTGTGTGGA AAAGGGTGAA GGCCAAGCTG
    GAGGGGAGAG ATGTGGATCC TAACAGGAGA ATGAGTGTCA CTGAGCAGGT GGATTATGTC
    ATCAAAGAGG CAACAAACGT GGACAACCTG GCACAGTTGT ACGAGGGATG GACGGCATGG
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45857
    Clone ID Related Accession (Same CDS sequence) XM_009299454.3 , XM_009299454.2
    Accession Version XM_009299454.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase SMG1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299454.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    10921
    ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG 
    AATGACTGGC AGCCCAGAAG CGACAGTCTG TCAGCCAGCC AGGACGGTGT GAAGTGCAGT
    GTCTCTAGAG ACCGCGGTTC AGAAGTTCTA TTTGAAATTC TGCCCTCTGA CCGGCCTGGC
    ACCCCACCAC TGCACAATGA CGCCTTCACT GCCACCGATG CACTCGAAGA GGGAGGTGGA
    TACGACGCTG ATGGAGGCCT CTCTGAAAAT GCTTATGGTT GGAAGTCGCT GGGGCAAGAG
    TTGAGAATTA ATGATGTGAC TACTGATATA ACCACTTTTC AGCATGGAAA CCGTGCTTTA
    GCTACAAAGG ACATGAGGAA ATCACAGGAC CGACCGTTGG CTCACTCTGA GGAGTCTCGT
    CTGGCCAATC TTCTGCGGAG AGCATCACGG GAAGATGACC GGGAACGCAG ACTGGCCACT
    ATGAAACAAA TGAAGGAGTT CATTGTGCAC TCTGAAAATA AAGTGGTCCT AGTGAAACAG
    TTGGATTCCA TTTTGAGCAC CCTGAATGAC ATTTTGAATG AGAGTAGTAA GCTCCTGCAG
    GAGCTCAGAC AGGAGGCCGC TTGGTGTCTG GGATTGCTCT GTGCCGCCCT CAGTTATGAA
    GCTGAGAGGA TCTTCAAGTG GATGTTCCTC AAGTTCAGTG TGTCTACAAA GGATGAGGTG
    AAGCTTCTGT ACCTTGTGGC AGTGCACAAA GCACTTGAAA CAGCTGGGGA GAAGAAGGCC
    TTTTCAGCTG TAATGCAGCT GGTGATGAGC AACCTTCAGT CTATATTGGA GAACTTGGAC
    ACACCGGAAC TATTGTGCCA AAGTGTAAAG TGTATTTTAC TGGTGGCCCG TTGTTATCCA
    CACATCTTCA GCACCAACTT CAGAGACACA GTGGATATCT TGGTAGGCTG GCATATAGAT
    CACACACAGA AGCACTCTCT TACTCAACAA GTATCTGGCT GGTTGCAGAG TTTGGAGCAA
    TTTTGGGTTG CAGATTTGGC CTTCTCGACC ACATTATTGG GTCAGTTTTT GGAAGATATG
    GAGGCATATG CTGAGGACCT GAGCCATGTT GTATCTGGAG AATCAGGAGA TGAGGACATT
    CCCCCTCCCA CTGTGTCCTT GCCTAAATTG GCAGCCCTAT TGAGAGTTTT TAGCACTGTT
    GTGCACAGCA TCGGAGAACG GTTTAACCCA ATCCGAGGCC CTCCAATTAC AGAAGCATAT
    GTCACTGATG TTTTGAATCG TGTTTTGGCT TGCGTCACCA CAGCCAAGCA GGTGTTCTTC
    TCTGAGGCGG TGCTGACAGC AGGAAATGAG TGTGTGTGTG TTCTGCTGGT GAGCATAGAC
    TTGGGCGGCC AGCTGATTGA TGCTGTAATC TCATACGGCC TAGACCAGCT GAACTGCTGC
    CAGAACTGCG GACCCGAATA CAGCCTGTCT GTTCTCACCC TGCTCACACT GGTTGTTGAC
    CAAATAAACA CCAAACTTCC GGCTTCTTTT GTGGAAAAGT TGTTGGCCCC AAAATCTCAT
    CTTTTGGAGC TGCGCTTTCA CAGAGAGAGA GAGGTGATGG CGGCAGCACA TGGAGTGTAT
    CATGCTGTGC TAAGCCTGAA GAACATCCCT ATCTTGGAGG CGGCATATAA ACTGGTCTTG
    GGCGAGATGG GCTGTGCACT GAACAGTTTG CTGAGCCCTC TGGGTTTGCC TGATGCCTGT
    CCTCACATCC AACATCCTGC TTTCAGCCAG CTGAACTTTA GCCCAGAGAG AGCTGAGTTT
    GTCCTCATCT TTAACCTCAG TGCTCTCACC ACCATTGGCA ACACCAAGAA CTCCCTCATC
    GGGATGTGGG CTCTGTCTCC TACTGTGTTT GCCCTGCTCA GTCAGAATCT GGTTATTGTT
    CATTCTGATT TGGCAGTTCA TCATCCTGCT GTCCAGTATG CAGTGCTCTA CACTCTCTAC
    TCTCACTGCA CCAGACATGA TCACTTCATC TCCAGCAGCC TGAGCTCCTC GTCCCCATCC
    CTTTTCGACG GTGCTGTCAT CAGCACAGTT ACCACGGCAA CCAAAAGGCA TTTCAGCACG
    CTCCTTAATT TGCTGGGTAT GCTGCTCAGC AAGGATCATC TCAACCCTGA GGCGAGGCGG
    CTGTTACTCA CCTGGTCATT GGAAGTAGCT CTGATGATGA AGAAATCTGA AACTTACGCT
    CCTCTCTTCT CGCTCCCATC CTTCCTCAAA TTCTGCAAAG GGTTACTGGC AAATTCCTTG
    AATGAGGACA CCACCATATG TCTGCAGGCA TGTAACAGTC TACAGGTGCT GTCATCTTCC
    TTGACTATGG AGCTTCTCCA GAGGTGTGTG GACGTGTGCC GAGTTCAGCT GGTTCACTCA
    GCAGTCCGAG TCCGGCAGGC CTTTGGGAAA TTGCTGAGAT CTGTTCCCAT GCACGTCGCA
    CTGAGCGCCC ATAGTCACTC TGAGATTAAG GAGATTTCTC TGGCCATCCG CCGACACATG
    AGCAAAGTGC CTAGCAACAC ATTCCACCCA CAGGACTTCT CTGACCTTAT TGGGTTTATT
    CTGTACGGAA CGGTCCATCG TGGGGGGAAG GAGCCCTGGT TGGAGCGTCT GTATCACAGC
    TGTCAGCGGC TGGAGAAGAA AGACTCTGCT ATGGTGCCTC GTGCCCTGCT GAAGACTGAG
    GCAGTGCTGT GGCAGTGGGC GGTGTGGGAA GCTGCTCAGT TTACTGTTCT CTCAAAGCTC
    CGTACACCTC TGGGAAGAGC CCAGGATACC TTCCAGACTA TAGAGGGTAT GATCCGTAGT
    CTGGCAGCTC ACAGTCTGAA CACAGAGCAG GAGCTGAGTC AGTGGAGCGG AGGAGAGAGT
    GATGAGGGTC ACCACACCAA CCAGCTCCGA CTGGCTCTTC TCCTGCAGTT CCTGGAGAAC
    CTGGAGAAAC TCATGTACAA CGCCTATGAA GGCTGTGCCA GTGCTCTCAC TGCACCACCA
    AAGGGCATTA GAACCTTTTT CTACACAAAC AGACAGACAT GCCAGGACTG GCTAACCAGA
    ATTCGTTTGG CCCTAATGAG GGTTGGCCTT CTCTCCGGTC AGCCAGCGGT CACAGTCCGT
    CATGGCTTTG ACCTGCTGAC TGAGATTAAA AACAGCAGTA CACAGGGTCC AGAAATGGAG
    GTTCCAGTGA CCATGCTGGT GGAGGCATTG TGCGAGTTGA GATGCCCAGA GGCTATTCAA
    GGTCTGGCAG CATGGAGTCT TGCCAACACT GGCAAAAGCA TGGGCTGGCT GAGCTCTGTG
    GCACTGCAGG CTGAGGGAAA GTTTGAGAAG GCAGCGTTAG AGTACCAAGA GCAGCTTTGT
    GCTGTCACAG GGATGGACTG CTCCATTAAA GTCTTTGATC GCTCACTTTT GAAACTCACT
    GGCACCAACA CCTCCAGTCC AAAACATACC AGCAGCGGAG AGGGAAGAAA AACTGTATTG
    CTGAAATCCA GCGAGTGTTC TCCAGAGGTT CTGAACTTTC TGGCTAATAA GGCCTGTGAG
    TGTTATGTGG CCCTGAGCGA TTGGGAGTCT GTGCAAGAAT GGCAGGCCAG CATGATGAAT
    CTCAAAAAGA ACAGCAGCAG TTCCAGTGTC AACCTCAAGA CAGACTTCAA CTACATCAGA
    GCTATGAGCA GGTTTGAGGA GGGAGATTTT ACAGAATGCC GTGCCCAGCT AGAGTTGCTC
    CCTGGGGATG ATTATGGCCT TCTCAACTCT ACAACTAAGG ACAAGATTGA CCTTAAGAGG
    CTTCTTCCTG CTGTGTTGAG TCCAGACCCA TCAGAGCTCC AGAAGGCCAT TGAGGTGCAG
    CTCCTCAGAA GTGCTGTGGG AGCCATCAGT GCGACCAATC ATGAGCAGGA CCAGAAAGTG
    GCATCATCAG ACACCCTGGT TAAATATCTG AAGCAGACGG GTCGCATCTG TCTGGGCCCC
    CTGCGCCTTT CCACTCTCAC CCTGTCAGAT TCCCTGCCCA CCCTTAGCAC TCTACAGCTG
    CACTGTGCCA ACTCTTTAGA GAACTCTCTT TGCAACCAAC ATCCTGAGGA CTGCCTTATT
    CCGCTCTTCA GTGAAGCTTT GACCACCTGC AAGCAGCAGG ATGTTCAACC ATGGCTGCAT
    GCTCTCCGAT ACACTACTTT TCAGCGGGAA TTCTTCCAAA AACTTAAAGG TTCATCTTCT
    CCAGTTGATT CTCATCTGAT GGAGCTTTGT CTGACTGCTG TAAAATTTGC TCGTAAACAA
    GGGAATATTG CTCTGGCCAC ACGCCTGCTG AGTCTATGCA GCAAGCCAGC TATGAGCGAC
    ACTGAAGGTC AGGACCTGGT TCAAAGCTTC AGGCAGCTGT CTCTAGAAGG CACTGTTGGG
    GAAAAGTGGG GACCAGAGCT GGAGATCGAG AAGGCTAAAG TTCTGTTCGC TGCTGGTCAG
    TCTGTGTCTG CTATGGAGAT GTTGAGCTCC TGTGCACTGT CATACTGTCA CTCTGGGAAG
    TGCGAACTTG CGGCCTGTCG CTCGGTGCTC ACTCTGTGCA AATGGCTTTT GGCTGACTGG
    AAAGATCTGA CTCCCCAGCT GAAGATGGTG GTGAAGAAGA ACTCTGGGTC TACCAGCCTC
    TCAACTCTCT CCAAAAACAT CTCTGGCTTG TTGGAGCTGC CGCTGGAAGA TCAGGGAATG
    CCACACATTA CAACAGAGAC TACAGTTAGC GTTGGTGTCG GGGAGCCAGA CTTTGTCCTT
    GGGCAACTTT ACCAGCTCTC AACTACTCAG GCTCCTGAAG TGGCCAAATC TTGGGCTGCT
    CTTGCCAGCT GGGCCTACAG ATGGGGCAGG AAGGTTGTGG ACAATGCCAG TCAAGGAGAA
    GGGGTTCCTC TGCTCCTCGG AGAGAAGAAA GAAATAGAGG AGTTGCTCCC AGCAGGCACC
    AGCGATGAGG ACAAGGAAAC CATTTTTGGC ATTTTGGGTC AAGCCATGTG TCGCCCAGCA
    GGAATTCAGG ATGAGGATAT GGCTCTGCAG AATGAAGAAG ATGATGAGGA CGACATGGTA
    GATGTAATTG GCCGACAGCT GTTGGGTGCC TGTCCCTGGC TTTCAGATGT AGAGGACACT
    GTGACCGATG GACTCATTGG GGTGTGGAGG AGAGTGGTTG ATCGGATCTT CAGCCTCTAC
    CGGGTCTCCT GCAGGGCTTA CTTCACTTTC CTCAAACTCA ATGCTGGACA GGTGCCTATA
    GATGAAGATG ATCCCAAGCT GCTCCTGAAC AACCAGAACA GCAAGCAGAG CAGCGATGAT
    GTCATTGTCA TGGCAACCCT CCGTCTGCTG CGCTTGTTGG TGAAGCACGC AGGAGAGTTG
    AGGGAAGGTC TGGAGCACGG TTTGGCATCC ACACCTACTG CTCCCTGGAG AGGTATTATC
    CCTCAGTTAT TCTCCAGACT TAATCACCCT GAAGCCTACA TCCGCCAGAG CATCTGCAGT
    CTGCTGTGTA GAGTGGCCCA AGATTCCCCA CACCTTATCC TTTATCCTGC CATTGTCGGA
    TCAATCTCTC TTGGTGGAGA GGCTCAGACG GCAGGTAATA AGCTGCCATC GTCCCTGCCA
    ACCCTGCTGG GGAACATGCA GGGAGAGGGT CTTTGTGGAG GTGAGAGCGA GACAGGCAGT
    GGGCCGACCT CTCAGGAGAG CAGCCGCGGG GAAGAGATGG TGATGTATTC CAGCGAGGAT
    CAAGCCATGA TGCAGGACTG CTACAGCAAG ATAGTAGACA AGCTTTCCTC TGCCAACCCT
    ACCATGGTGC TGCAGGTGCA GATGTTGGTG GGCGAGTTGA GGAGGGTCAC ACTGCTCTGG
    GACGAGCTCT GGCTGGGGGT CTTACAGCAG CAGCACATGC ACGTCCTGCG CAGGATTCAA
    CAGCTGGAGG ACGAGGTGAA GAGAGTTCAG AACAACAACA CTCTGCGCAA GGATGAGAAG
    GTGGCTATTA TGAGAGAGAA GCACTCTGCT CTGATGAAGC CTGTGGTTTT TGCGCTGGAT
    CATGTGCGCA GCATTACGGC TGCTCCAGCA GAGACGCCTC ACGAGGAATG GTTTCAGGAG
    ACATATGGAG ATGCCATCCA CAATGCCCTA GAGAGACTGA GGAGCCCTCT AAATCCAGCC
    AATCCTGCCA GCAGCTGGGT GCCCTTTAAA CAGATCATGC TGAGTTTACA GCAGAGGGCA
    CAGAAACGAG CCAGTTACCT TCTGCGTCTG GATGAGATCA GCCCACGTCT GACTGCTATG
    GCTAACACCG AGATGGCTCT GCCGGGAGAG GTGTCTGCTA CAGATGCAGT CACCATCCAG
    AGTGTGGGCA ACACCATCAC CATTCTGCCA ACTAAAACCA AACCCAAGAA GCTCTACTTT
    TTGGGATCGG ATGGGAGAAA CTATCCTTAT CTGTTCAAAG GATTGGAGGA TCTGCATTTG
    GATGAGAGGA TCATGCAGTT CTTGTCTATC GTCAACACCA TGTTCACTAA AGTGAATCAG
    CAGGAGAGCC CGAGGTTTCA AGCCCGTCAT TATTCTGTCA CACCTCTGGG TACCAGATCT
    GGCCTCATCC AGTGGGTGGA TGGAGCCACT CCACTGTTTG GCCTGTACAA ACGTTGGCAG
    CAGCGTGAAG CTGTGGTCCA AGCCCAGAAG GCACAGGACT CCTTCCAGCA GCCTCAGAAT
    CTTCCCATGG TCCCACGGCC CAGTGAGCTT TACTACAGTA AAATCAGTCC TGCCTTAAAG
    GCTGTCGGTC TCAGTTTGGA TGTGTCACGT CGTGATTGGC CGTTAAGTGT TATGAGAGAT
    GTTCTAAGAG AGCTGATGGA AGCCACGCCC CCTAACCTCT TGGCAAAGGA GCTCTGGTGT
    TCCTGCACCA CTCCTAGTGA GTGGTGGAGC GTCACTCAGA CTTACGCCAG ATCCACCGCT
    GTGATGTCAA TGGTGGGATA CATTATTGGC TTGGGGGATC GTCACCTTGA CAACGTGCTG
    ATTGACATGA CGACTGGCGA GGTGGTGCAC ATTGACTATA ACGTCTGTTT TGAGAAGGGA
    AAAAGCTTGA GAGTGCCTGA GAAAGTTCCT TTTCGCATGA CCCACAACAT CGAGACTGCT
    CTGGGGGTCA CAGGAGTTGA GGGAATCTTT AGACTGTCCT GTGAACAGGT GGTCCAGATA
    ATGCGGCGTG GGAGAGAGAC CCTCTTGACT CTTCTAGAGG CCTTTGTCTA TGACCCATTG
    GTGGACTGGA CAGCAGGCGG TGAGGTGGGC TTTGCAGGTG CTGTGTATGG AGGAGGTGGT
    CAGCAGGCTG AGAACAAGCA GAGCAAGAGA GAGATGGAGA GAGACATCAC ACGTAGCCTT
    TTCTCCTCCC GTGTGGCTGA AATTAAGGTG AACTGGTTCA AAAACCGTGA TGAAATGACT
    GGAGTGTTGC CACAGCTGGA GGAGGCAGTG GATGAGTACC TCAACCTGCA GGAGCAGCTC
    ACACAAGTGG AGAAGGTGCA GGGCAAGCTG TTGGAGGAGT TGGAGTTCCT GGAGGGAGCA
    GACACCAGAG CAGACCATCC CATCCACTCC CTGGAGCACA GATACTCAGA ACACACGCAG
    CTGCAGTCCA GACAGAGAAC TGTGCAGGAT GCCATTCAAG GGAAGTTGTC AGATCTGGAT
    CAATGGATCT CTCAGTATCA AGCTGCCTTT GCCAGTTTAG AAGCCACACA GCTTGCCAGC
    CTCCTCCAGG AGATCAGTAG CCCTATTGAT TTGGGTCCGC CCAGTTATGT ACCAGCAACG
    TCGTTCTTGC AGAATGCAGG TCAGGCACAT CTGATCAGCC AGTGTGAAGC GCTGGAGGCT
    GAAGTGAGTG CTCTGTTACA GCAGAGGCGC TCTCAGCTGC GAGGTTGTCT TGAGCACCTG
    CACAGTTATG CCACCGTGGC CCTGCTGTAC CCACGTGCCG TGCTGCACAG ACACAGAGCT
    CACACATGGA AACAGTGGAT GGAGGAGCTG GTGTGTGACA TGACGGTCGA TCACTGCCAG
    ACTATTTATC ACCAATACGA AATGCAGTTT GCACCCCAGC CCCCACCTGC CACGTGTCAG
    TTCCTCTCCA GCATCGAGAT GGCGCTGCAG CACCATGCAG CAGAAACCAA CACTCGTCTG
    CTGCGGCAGG TGGAGCGTCT GAAGGCAGAG GGCGCCAGTG TACCCGTGTG TGAGGAGCAG
    CTGCAGGAGA TCGAGCGCTG CATCAAAGTC TTCCTGCACG AGGACGCTGA GCTTGGCTCC
    TTCAGCCTGG CTGGCATCAT CGTCTCTGCC CTCTGTTCTC TCACCAGGCG TAATTTGGTG
    ATGGAGGGAG CTGCGGCCAG TGCTGGCGAG CAGCTGGTGG AGCTGACGTC TCGTGATGGA
    GCCTGGTTCC TAGAGGAGCT CTGCAGCATG AGTGGAAACA TTACATGTCT TGTGCAGCTG
    TTGCAGCAGT GCCAGCTTCT GTCACATGAC CTGGACATCC CTAGTCCTGC AGAAACTTCG
    CAAGTTGTCT ACCTGACCAA TGGTGTTTAC ACCTGTCTTC AGGAGCTGAA CACAAACTTC
    CGTCAGATCA TCTTCCCAGA GGCTCTGCGC TGCATGCTGA AAGGTGAAAA CACTTTAGAG
    ACCATGTTAG CAGAGCTGGA TGCGCTTATC GATCAGTGTG CAGATGGGGT TTCACTGCAG
    GGGCTTGGAG AGATTTTGAT GGCACACATT CGGAACGCCT CCATGGGTCT GGAAGAGGAT
    CCAGATGACC ACTATCTGGA CGTTACCAGG GTTTTGCGAG CCCAGTACTC TGAGCTGATT
    CAGCCCCGCA GCATGGAGAG CTCAGTGCAG GAAACGCCAA AGATGAGCGC TGGTCAGATG
    TTGCTGGTAG CTTTTGATGG CATGTTTGCC CAGTTGGAGA CAGCTTTTGG ACTTCTCATT
    GACAAGCTGA ACTCTATGGA TGTCCCTGCT GCCTGGAGAA AAGTGGATGT GATCCGGGAG
    TCGAGAGCCA CACAGGCTCA TTTCTTTGAT AATGTCCAGA CCAGGCAGGT CTTGGAGGAG
    ATTTTCTTCT TGAAGAGACT CCAGACCATC CGAGACTTCT TCCGTCTATG TGGTTCATTT
    GCACAGACAC TATCTGGCAC ATGCCCTACA CCCACTGATG ACCCTCCGCC ATCTAATGGC
    CCTGTTCCCA TAGTAAAGCC CTTGTATCGA GGCTCCACGG TGGTGAGCGA GGACCAGATG
    ACGCGGCCCA TCAAAGCCTT TACAGCAGAC TTTGTAAGGC AGATGCTGAT GGGGCTCCCC
    ACCCAGGCTC TGGGTCTAGC CATCTGCAGC TCTCTCAGTG CCCTGGGCAT GGATCTTATT
    GCTCAAGTGG AGGCCAAGGA CTTTGGAGCT GAGGGAAAAG TCTCTCTGGA TGACCTGTGC
    AAGAAAGCTG TAGAGCAGGG GGTGCAGGCA GGACGCCTGT CCCAGCTGCT ACTCAACAGG
    GCCACCGTGC TGGCCAGTTC CTATGACACT GCCTGGAAAA AACTTGACCT GGTCCGACGT
    CTGGAGGTCA GCATTGAAGC CTGTAAGGTC AGCCTGCAGA GGAATCAGCT TCACATCGCC
    ATGTTCCAGT GGCAACATGA GGACATTCTT GGTGCGAGGA CGCAGCCCAT GACAGTCAGT
    CCTCCACCAC GTTCCATTAT CCTCAGCAAC ATGAAAAAGA AGCTCTACAA ACTCAGTCAG
    GATGACGCTT CAATAGGAAG TGTCCAGGAG AAGTTGGCCA GTCTTGAAGG CAGTATAGAG
    CAGAGGCTGA AATGGGCCGG TGGTGCAAAT CCTGCACTCG CTCCAGTGCT GCAGGACTTT
    GAGGCCACTA TAGCAGAACG CCGTGCCTTG GTGATAAAGG AGAGTCAGCG TGCCAACCAG
    GTCACTTTCC TCTGCAGTAC CATTCTCAAC TTTGAAGGTC TGCGCACCCG CACACCCGAA
    GCTTTGAACA TGGATGCTGC CCTTTTTGAG CTTGTGAAAC GATGCCAGGC CACCTGCTCC
    TACGCTGCCC AGTTCAATAC CTCTGTTTCC TCGCTAGAAC TACAGCTGCT GCATAGACTG
    AGTCCTGCCA TGGATATGTC CATTGGTGGT CCTGAGTGGC TGGTGTATGC ACAGAATCAC
    CTGACCCAGG AGATGTCCAG TCAGAGAGCC ATGCAGGAGG AAAGAGAGCA GCAGCTGGAG
    AGCGTGACTG AGACCCTGCA GCTGCTGGTG GACTCCATTA AAGGCACCCT GTCCAATCAC
    AACCGCCAGC TCGCTGACGT TAAACACTTA CTGCGAGCAA TGGCAAAGGA TGAAGAGAAT
    GCCCTTGCAG AAGGAGAGGA AGTGACTTAT GATGGGAGTG TGCGTCAGTT CCTTTCTGAA
    TACAAGGCAT GGCAGGATAA TGTGCAGATA GTGCTGTTCA CAGTAGTGCA GGCTACAGGG
    CAACCACGCA GTCAAGAGCA GATCGAGCTG CTGCAGGAGA TTCCCGCCAC CTTGAAGGAG
    CTTAAGGTTC AGAGCCACAG CATCTACAAT GGATTGGTGG GCTTTGCCTC TCCCCTTGTG
    ACAGAGAGAG GAAGTGACTG TATTAGTCCC ACTTCCACAG TCCAGACCAG CTTTGCCGCT
    GCAGTGCGCT GCAGTGGGGT GAAGACACAG CCTGACAGTA TGTCTCAGAA TGCACGCAAA
    GCTCTTCCTC GAAACTTTGG GACCCCGGCG GACACTCCTC CCAGCACACT GATGATCAAC
    AGCAAAGGAC TGGCCCCTAG CCCCAAAAGA GCCGTGCGAG ATCCCAAAAC TGGGAGAGCT
    GTGCAAGAGA GAAATTCATA TGCTGTTAGT GTGTGGAAAA GGGTGAAGGC CAAGCTGGAG
    GGGAGAGATG TGGATCCTAA CAGGAGAATG AGTGTCACTG AGCAGGTGGA TTATGTCATC
    AAAGAGGCAA CAAACGTGGA CAACCTGGCA CAGTTGTACG AGGGATGGAC GGCATGGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297729.1
    CDS181..11103
    Translation

    Target ORF information:

    RefSeq Version XM_009299454.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio SMG1 phosphatidylinositol 3-kinase-related kinase (smg1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299454.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG 
    AATGACTGGC AGCCCAGAAG CGACAGTCTG TCAGCCAGCC AGGACGGTGT GAAGTGCAGT
    GTCTCTAGAG ACCGCGGTTC AGAAGTTCTA TTTGAAATTC TGCCCTCTGA CCGGCCTGGC
    ACCCCACCAC TGCACAATGA CGCCTTCACT GCCACCGATG CACTCGAAGA GGGAGGTGGA
    TACGACGCTG ATGGAGGCCT CTCTGAAAAT GCTTATGGTT GGAAGTCGCT GGGGCAAGAG
    TTGAGAATTA ATGATGTGAC TACTGATATA ACCACTTTTC AGCATGGAAA CCGTGCTTTA
    GCTACAAAGG ACATGAGGAA ATCACAGGAC CGACCGTTGG CTCACTCTGA GGAGTCTCGT
    CTGGCCAATC TTCTGCGGAG AGCATCACGG GAAGATGACC GGGAACGCAG ACTGGCCACT
    ATGAAACAAA TGAAGGAGTT CATTGTGCAC TCTGAAAATA AAGTGGTCCT AGTGAAACAG
    TTGGATTCCA TTTTGAGCAC CCTGAATGAC ATTTTGAATG AGAGTAGTAA GCTCCTGCAG
    GAGCTCAGAC AGGAGGCCGC TTGGTGTCTG GGATTGCTCT GTGCCGCCCT CAGTTATGAA
    GCTGAGAGGA TCTTCAAGTG GATGTTCCTC AAGTTCAGTG TGTCTACAAA GGATGAGGTG
    AAGCTTCTGT ACCTTGTGGC AGTGCACAAA GCACTTGAAA CAGCTGGGGA GAAGAAGGCC
    TTTTCAGCTG TAATGCAGCT GGTGATGAGC AACCTTCAGT CTATATTGGA GAACTTGGAC
    ACACCGGAAC TATTGTGCCA AAGTGTAAAG TGTATTTTAC TGGTGGCCCG TTGTTATCCA
    CACATCTTCA GCACCAACTT CAGAGACACA GTGGATATCT TGGTAGGCTG GCATATAGAT
    CACACACAGA AGCACTCTCT TACTCAACAA GTATCTGGCT GGTTGCAGAG TTTGGAGCAA
    TTTTGGGTTG CAGATTTGGC CTTCTCGACC ACATTATTGG GTCAGTTTTT GGAAGATATG
    GAGGCATATG CTGAGGACCT GAGCCATGTT GTATCTGGAG AATCAGGAGA TGAGGACATT
    CCCCCTCCCA CTGTGTCCTT GCCTAAATTG GCAGCCCTAT TGAGAGTTTT TAGCACTGTT
    GTGCACAGCA TCGGAGAACG GTTTAACCCA ATCCGAGGCC CTCCAATTAC AGAAGCATAT
    GTCACTGATG TTTTGAATCG TGTTTTGGCT TGCGTCACCA CAGCCAAGCA GGTGTTCTTC
    TCTGAGGCGG TGCTGACAGC AGGAAATGAG TGTGTGTGTG TTCTGCTGGT GAGCATAGAC
    TTGGGCGGCC AGCTGATTGA TGCTGTAATC TCATACGGCC TAGACCAGCT GAACTGCTGC
    CAGAACTGCG GACCCGAATA CAGCCTGTCT GTTCTCACCC TGCTCACACT GGTTGTTGAC
    CAAATAAACA CCAAACTTCC GGCTTCTTTT GTGGAAAAGT TGTTGGCCCC AAAATCTCAT
    CTTTTGGAGC TGCGCTTTCA CAGAGAGAGA GAGGTGATGG CGGCAGCACA TGGAGTGTAT
    CATGCTGTGC TAAGCCTGAA GAACATCCCT ATCTTGGAGG CGGCATATAA ACTGGTCTTG
    GGCGAGATGG GCTGTGCACT GAACAGTTTG CTGAGCCCTC TGGGTTTGCC TGATGCCTGT
    CCTCACATCC AACATCCTGC TTTCAGCCAG CTGAACTTTA GCCCAGAGAG AGCTGAGTTT
    GTCCTCATCT TTAACCTCAG TGCTCTCACC ACCATTGGCA ACACCAAGAA CTCCCTCATC
    GGGATGTGGG CTCTGTCTCC TACTGTGTTT GCCCTGCTCA GTCAGAATCT GGTTATTGTT
    CATTCTGATT TGGCAGTTCA TCATCCTGCT GTCCAGTATG CAGTGCTCTA CACTCTCTAC
    TCTCACTGCA CCAGACATGA TCACTTCATC TCCAGCAGCC TGAGCTCCTC GTCCCCATCC
    CTTTTCGACG GTGCTGTCAT CAGCACAGTT ACCACGGCAA CCAAAAGGCA TTTCAGCACG
    CTCCTTAATT TGCTGGGTAT GCTGCTCAGC AAGGATCATC TCAACCCTGA GGCGAGGCGG
    CTGTTACTCA CCTGGTCATT GGAAGTAGCT CTGATGATGA AGAAATCTGA AACTTACGCT
    CCTCTCTTCT CGCTCCCATC CTTCCTCAAA TTCTGCAAAG GGTTACTGGC AAATTCCTTG
    AATGAGGACA CCACCATATG TCTGCAGGCA TGTAACAGTC TACAGGTGCT GTCATCTTCC
    TTGACTATGG AGCTTCTCCA GAGGTGTGTG GACGTGTGCC GAGTTCAGCT GGTTCACTCA
    GCAGTCCGAG TCCGGCAGGC CTTTGGGAAA TTGCTGAGAT CTGTTCCCAT GCACGTCGCA
    CTGAGCGCCC ATAGTCACTC TGAGATTAAG GAGATTTCTC TGGCCATCCG CCGACACATG
    AGCAAAGTGC CTAGCAACAC ATTCCACCCA CAGGACTTCT CTGACCTTAT TGGGTTTATT
    CTGTACGGAA CGGTCCATCG TGGGGGGAAG GAGCCCTGGT TGGAGCGTCT GTATCACAGC
    TGTCAGCGGC TGGAGAAGAA AGACTCTGCT ATGGTGCCTC GTGCCCTGCT GAAGACTGAG
    GCAGTGCTGT GGCAGTGGGC GGTGTGGGAA GCTGCTCAGT TTACTGTTCT CTCAAAGCTC
    CGTACACCTC TGGGAAGAGC CCAGGATACC TTCCAGACTA TAGAGGGTAT GATCCGTAGT
    CTGGCAGCTC ACAGTCTGAA CACAGAGCAG GAGCTGAGTC AGTGGAGCGG AGGAGAGAGT
    GATGAGGGTC ACCACACCAA CCAGCTCCGA CTGGCTCTTC TCCTGCAGTT CCTGGAGAAC
    CTGGAGAAAC TCATGTACAA CGCCTATGAA GGCTGTGCCA GTGCTCTCAC TGCACCACCA
    AAGGGCATTA GAACCTTTTT CTACACAAAC AGACAGACAT GCCAGGACTG GCTAACCAGA
    ATTCGTTTGG CCCTAATGAG GGTTGGCCTT CTCTCCGGTC AGCCAGCGGT CACAGTCCGT
    CATGGCTTTG ACCTGCTGAC TGAGATTAAA AACAGCAGTA CACAGGGTCC AGAAATGGAG
    GTTCCAGTGA CCATGCTGGT GGAGGCATTG TGCGAGTTGA GATGCCCAGA GGCTATTCAA
    GGTCTGGCAG CATGGAGTCT TGCCAACACT GGCAAAAGCA TGGGCTGGCT GAGCTCTGTG
    GCACTGCAGG CTGAGGGAAA GTTTGAGAAG GCAGCGTTAG AGTACCAAGA GCAGCTTTGT
    GCTGTCACAG GGATGGACTG CTCCATTAAA GTCTTTGATC GCTCACTTTT GAAACTCACT
    GGCACCAACA CCTCCAGTCC AAAACATACC AGCAGCGGAG AGGGAAGAAA AACTGTATTG
    CTGAAATCCA GCGAGTGTTC TCCAGAGGTT CTGAACTTTC TGGCTAATAA GGCCTGTGAG
    TGTTATGTGG CCCTGAGCGA TTGGGAGTCT GTGCAAGAAT GGCAGGCCAG CATGATGAAT
    CTCAAAAAGA ACAGCAGCAG TTCCAGTGTC AACCTCAAGA CAGACTTCAA CTACATCAGA
    GCTATGAGCA GGTTTGAGGA GGGAGATTTT ACAGAATGCC GTGCCCAGCT AGAGTTGCTC
    CCTGGGGATG ATTATGGCCT TCTCAACTCT ACAACTAAGG ACAAGATTGA CCTTAAGAGG
    CTTCTTCCTG CTGTGTTGAG TCCAGACCCA TCAGAGCTCC AGAAGGCCAT TGAGGTGCAG
    CTCCTCAGAA GTGCTGTGGG AGCCATCAGT GCGACCAATC ATGAGCAGGA CCAGAAAGTG
    GCATCATCAG ACACCCTGGT TAAATATCTG AAGCAGACGG GTCGCATCTG TCTGGGCCCC
    CTGCGCCTTT CCACTCTCAC CCTGTCAGAT TCCCTGCCCA CCCTTAGCAC TCTACAGCTG
    CACTGTGCCA ACTCTTTAGA GAACTCTCTT TGCAACCAAC ATCCTGAGGA CTGCCTTATT
    CCGCTCTTCA GTGAAGCTTT GACCACCTGC AAGCAGCAGG ATGTTCAACC ATGGCTGCAT
    GCTCTCCGAT ACACTACTTT TCAGCGGGAA TTCTTCCAAA AACTTAAAGG TTCATCTTCT
    CCAGTTGATT CTCATCTGAT GGAGCTTTGT CTGACTGCTG TAAAATTTGC TCGTAAACAA
    GGGAATATTG CTCTGGCCAC ACGCCTGCTG AGTCTATGCA GCAAGCCAGC TATGAGCGAC
    ACTGAAGGTC AGGACCTGGT TCAAAGCTTC AGGCAGCTGT CTCTAGAAGG CACTGTTGGG
    GAAAAGTGGG GACCAGAGCT GGAGATCGAG AAGGCTAAAG TTCTGTTCGC TGCTGGTCAG
    TCTGTGTCTG CTATGGAGAT GTTGAGCTCC TGTGCACTGT CATACTGTCA CTCTGGGAAG
    TGCGAACTTG CGGCCTGTCG CTCGGTGCTC ACTCTGTGCA AATGGCTTTT GGCTGACTGG
    AAAGATCTGA CTCCCCAGCT GAAGATGGTG GTGAAGAAGA ACTCTGGGTC TACCAGCCTC
    TCAACTCTCT CCAAAAACAT CTCTGGCTTG TTGGAGCTGC CGCTGGAAGA TCAGGGAATG
    CCACACATTA CAACAGAGAC TACAGTTAGC GTTGGTGTCG GGGAGCCAGA CTTTGTCCTT
    GGGCAACTTT ACCAGCTCTC AACTACTCAG GCTCCTGAAG TGGCCAAATC TTGGGCTGCT
    CTTGCCAGCT GGGCCTACAG ATGGGGCAGG AAGGTTGTGG ACAATGCCAG TCAAGGAGAA
    GGGGTTCCTC TGCTCCTCGG AGAGAAGAAA GAAATAGAGG AGTTGCTCCC AGCAGGCACC
    AGCGATGAGG ACAAGGAAAC CATTTTTGGC ATTTTGGGTC AAGCCATGTG TCGCCCAGCA
    GGAATTCAGG ATGAGGATAT GGCTCTGCAG AATGAAGAAG ATGATGAGGA CGACATGGTA
    GATGTAATTG GCCGACAGCT GTTGGGTGCC TGTCCCTGGC TTTCAGATGT AGAGGACACT
    GTGACCGATG GACTCATTGG GGTGTGGAGG AGAGTGGTTG ATCGGATCTT CAGCCTCTAC
    CGGGTCTCCT GCAGGGCTTA CTTCACTTTC CTCAAACTCA ATGCTGGACA GGTGCCTATA
    GATGAAGATG ATCCCAAGCT GCTCCTGAAC AACCAGAACA GCAAGCAGAG CAGCGATGAT
    GTCATTGTCA TGGCAACCCT CCGTCTGCTG CGCTTGTTGG TGAAGCACGC AGGAGAGTTG
    AGGGAAGGTC TGGAGCACGG TTTGGCATCC ACACCTACTG CTCCCTGGAG AGGTATTATC
    CCTCAGTTAT TCTCCAGACT TAATCACCCT GAAGCCTACA TCCGCCAGAG CATCTGCAGT
    CTGCTGTGTA GAGTGGCCCA AGATTCCCCA CACCTTATCC TTTATCCTGC CATTGTCGGA
    TCAATCTCTC TTGGTGGAGA GGCTCAGACG GCAGGTAATA AGCTGCCATC GTCCCTGCCA
    ACCCTGCTGG GGAACATGCA GGGAGAGGGT CTTTGTGGAG GTGAGAGCGA GACAGGCAGT
    GGGCCGACCT CTCAGGAGAG CAGCCGCGGG GAAGAGATGG TGATGTATTC CAGCGAGGAT
    CAAGCCATGA TGCAGGACTG CTACAGCAAG ATAGTAGACA AGCTTTCCTC TGCCAACCCT
    ACCATGGTGC TGCAGGTGCA GATGTTGGTG GGCGAGTTGA GGAGGGTCAC ACTGCTCTGG
    GACGAGCTCT GGCTGGGGGT CTTACAGCAG CAGCACATGC ACGTCCTGCG CAGGATTCAA
    CAGCTGGAGG ACGAGGTGAA GAGAGTTCAG AACAACAACA CTCTGCGCAA GGATGAGAAG
    GTGGCTATTA TGAGAGAGAA GCACTCTGCT CTGATGAAGC CTGTGGTTTT TGCGCTGGAT
    CATGTGCGCA GCATTACGGC TGCTCCAGCA GAGACGCCTC ACGAGGAATG GTTTCAGGAG
    ACATATGGAG ATGCCATCCA CAATGCCCTA GAGAGACTGA GGAGCCCTCT AAATCCAGCC
    AATCCTGCCA GCAGCTGGGT GCCCTTTAAA CAGATCATGC TGAGTTTACA GCAGAGGGCA
    CAGAAACGAG CCAGTTACCT TCTGCGTCTG GATGAGATCA GCCCACGTCT GACTGCTATG
    GCTAACACCG AGATGGCTCT GCCGGGAGAG GTGTCTGCTA CAGATGCAGT CACCATCCAG
    AGTGTGGGCA ACACCATCAC CATTCTGCCA ACTAAAACCA AACCCAAGAA GCTCTACTTT
    TTGGGATCGG ATGGGAGAAA CTATCCTTAT CTGTTCAAAG GATTGGAGGA TCTGCATTTG
    GATGAGAGGA TCATGCAGTT CTTGTCTATC GTCAACACCA TGTTCACTAA AGTGAATCAG
    CAGGAGAGCC CGAGGTTTCA AGCCCGTCAT TATTCTGTCA CACCTCTGGG TACCAGATCT
    GGCCTCATCC AGTGGGTGGA TGGAGCCACT CCACTGTTTG GCCTGTACAA ACGTTGGCAG
    CAGCGTGAAG CTGTGGTCCA AGCCCAGAAG GCACAGGACT CCTTCCAGCA GCCTCAGAAT
    CTTCCCATGG TCCCACGGCC CAGTGAGCTT TACTACAGTA AAATCAGTCC TGCCTTAAAG
    GCTGTCGGTC TCAGTTTGGA TGTGTCACGT CGTGATTGGC CGTTAAGTGT TATGAGAGAT
    GTTCTAAGAG AGCTGATGGA AGCCACGCCC CCTAACCTCT TGGCAAAGGA GCTCTGGTGT
    TCCTGCACCA CTCCTAGTGA GTGGTGGAGC GTCACTCAGA CTTACGCCAG ATCCACCGCT
    GTGATGTCAA TGGTGGGATA CATTATTGGC TTGGGGGATC GTCACCTTGA CAACGTGCTG
    ATTGACATGA CGACTGGCGA GGTGGTGCAC ATTGACTATA ACGTCTGTTT TGAGAAGGGA
    AAAAGCTTGA GAGTGCCTGA GAAAGTTCCT TTTCGCATGA CCCACAACAT CGAGACTGCT
    CTGGGGGTCA CAGGAGTTGA GGGAATCTTT AGACTGTCCT GTGAACAGGT GGTCCAGATA
    ATGCGGCGTG GGAGAGAGAC CCTCTTGACT CTTCTAGAGG CCTTTGTCTA TGACCCATTG
    GTGGACTGGA CAGCAGGCGG TGAGGTGGGC TTTGCAGGTG CTGTGTATGG AGGAGGTGGT
    CAGCAGGCTG AGAACAAGCA GAGCAAGAGA GAGATGGAGA GAGACATCAC ACGTAGCCTT
    TTCTCCTCCC GTGTGGCTGA AATTAAGGTG AACTGGTTCA AAAACCGTGA TGAAATGACT
    GGAGTGTTGC CACAGCTGGA GGAGGCAGTG GATGAGTACC TCAACCTGCA GGAGCAGCTC
    ACACAAGTGG AGAAGGTGCA GGGCAAGCTG TTGGAGGAGT TGGAGTTCCT GGAGGGAGCA
    GACACCAGAG CAGACCATCC CATCCACTCC CTGGAGCACA GATACTCAGA ACACACGCAG
    CTGCAGTCCA GACAGAGAAC TGTGCAGGAT GCCATTCAAG GGAAGTTGTC AGATCTGGAT
    CAATGGATCT CTCAGTATCA AGCTGCCTTT GCCAGTTTAG AAGCCACACA GCTTGCCAGC
    CTCCTCCAGG AGATCAGTAG CCCTATTGAT TTGGGTCCGC CCAGTTATGT ACCAGCAACG
    TCGTTCTTGC AGAATGCAGG TCAGGCACAT CTGATCAGCC AGTGTGAAGC GCTGGAGGCT
    GAAGTGAGTG CTCTGTTACA GCAGAGGCGC TCTCAGCTGC GAGGTTGTCT TGAGCACCTG
    CACAGTTATG CCACCGTGGC CCTGCTGTAC CCACGTGCCG TGCTGCACAG ACACAGAGCT
    CACACATGGA AACAGTGGAT GGAGGAGCTG GTGTGTGACA TGACGGTCGA TCACTGCCAG
    ACTATTTATC ACCAATACGA AATGCAGTTT GCACCCCAGC CCCCACCTGC CACGTGTCAG
    TTCCTCTCCA GCATCGAGAT GGCGCTGCAG CACCATGCAG CAGAAACCAA CACTCGTCTG
    CTGCGGCAGG TGGAGCGTCT GAAGGCAGAG GGCGCCAGTG TACCCGTGTG TGAGGAGCAG
    CTGCAGGAGA TCGAGCGCTG CATCAAAGTC TTCCTGCACG AGGACGCTGA GCTTGGCTCC
    TTCAGCCTGG CTGGCATCAT CGTCTCTGCC CTCTGTTCTC TCACCAGGCG TAATTTGGTG
    ATGGAGGGAG CTGCGGCCAG TGCTGGCGAG CAGCTGGTGG AGCTGACGTC TCGTGATGGA
    GCCTGGTTCC TAGAGGAGCT CTGCAGCATG AGTGGAAACA TTACATGTCT TGTGCAGCTG
    TTGCAGCAGT GCCAGCTTCT GTCACATGAC CTGGACATCC CTAGTCCTGC AGAAACTTCG
    CAAGTTGTCT ACCTGACCAA TGGTGTTTAC ACCTGTCTTC AGGAGCTGAA CACAAACTTC
    CGTCAGATCA TCTTCCCAGA GGCTCTGCGC TGCATGCTGA AAGGTGAAAA CACTTTAGAG
    ACCATGTTAG CAGAGCTGGA TGCGCTTATC GATCAGTGTG CAGATGGGGT TTCACTGCAG
    GGGCTTGGAG AGATTTTGAT GGCACACATT CGGAACGCCT CCATGGGTCT GGAAGAGGAT
    CCAGATGACC ACTATCTGGA CGTTACCAGG GTTTTGCGAG CCCAGTACTC TGAGCTGATT
    CAGCCCCGCA GCATGGAGAG CTCAGTGCAG GAAACGCCAA AGATGAGCGC TGGTCAGATG
    TTGCTGGTAG CTTTTGATGG CATGTTTGCC CAGTTGGAGA CAGCTTTTGG ACTTCTCATT
    GACAAGCTGA ACTCTATGGA TGTCCCTGCT GCCTGGAGAA AAGTGGATGT GATCCGGGAG
    TCGAGAGCCA CACAGGCTCA TTTCTTTGAT AATGTCCAGA CCAGGCAGGT CTTGGAGGAG
    ATTTTCTTCT TGAAGAGACT CCAGACCATC CGAGACTTCT TCCGTCTATG TGGTTCATTT
    GCACAGACAC TATCTGGCAC ATGCCCTACA CCCACTGATG ACCCTCCGCC ATCTAATGGC
    CCTGTTCCCA TAGTAAAGCC CTTGTATCGA GGCTCCACGG TGGTGAGCGA GGACCAGATG
    ACGCGGCCCA TCAAAGCCTT TACAGCAGAC TTTGTAAGGC AGATGCTGAT GGGGCTCCCC
    ACCCAGGCTC TGGGTCTAGC CATCTGCAGC TCTCTCAGTG CCCTGGGCAT GGATCTTATT
    GCTCAAGTGG AGGCCAAGGA CTTTGGAGCT GAGGGAAAAG TCTCTCTGGA TGACCTGTGC
    AAGAAAGCTG TAGAGCAGGG GGTGCAGGCA GGACGCCTGT CCCAGCTGCT ACTCAACAGG
    GCCACCGTGC TGGCCAGTTC CTATGACACT GCCTGGAAAA AACTTGACCT GGTCCGACGT
    CTGGAGGTCA GCATTGAAGC CTGTAAGGTC AGCCTGCAGA GGAATCAGCT TCACATCGCC
    ATGTTCCAGT GGCAACATGA GGACATTCTT GGTGCGAGGA CGCAGCCCAT GACAGTCAGT
    CCTCCACCAC GTTCCATTAT CCTCAGCAAC ATGAAAAAGA AGCTCTACAA ACTCAGTCAG
    GATGACGCTT CAATAGGAAG TGTCCAGGAG AAGTTGGCCA GTCTTGAAGG CAGTATAGAG
    CAGAGGCTGA AATGGGCCGG TGGTGCAAAT CCTGCACTCG CTCCAGTGCT GCAGGACTTT
    GAGGCCACTA TAGCAGAACG CCGTGCCTTG GTGATAAAGG AGAGTCAGCG TGCCAACCAG
    GTCACTTTCC TCTGCAGTAC CATTCTCAAC TTTGAAGGTC TGCGCACCCG CACACCCGAA
    GCTTTGAACA TGGATGCTGC CCTTTTTGAG CTTGTGAAAC GATGCCAGGC CACCTGCTCC
    TACGCTGCCC AGTTCAATAC CTCTGTTTCC TCGCTAGAAC TACAGCTGCT GCATAGACTG
    AGTCCTGCCA TGGATATGTC CATTGGTGGT CCTGAGTGGC TGGTGTATGC ACAGAATCAC
    CTGACCCAGG AGATGTCCAG TCAGAGAGCC ATGCAGGAGG AAAGAGAGCA GCAGCTGGAG
    AGCGTGACTG AGACCCTGCA GCTGCTGGTG GACTCCATTA AAGGCACCCT GTCCAATCAC
    AACCGCCAGC TCGCTGACGT TAAACACTTA CTGCGAGCAA TGGCAAAGGA TGAAGAGAAT
    GCCCTTGCAG AAGGAGAGGA AGTGACTTAT GATGGGAGTG TGCGTCAGTT CCTTTCTGAA
    TACAAGGCAT GGCAGGATAA TGTGCAGATA GTGCTGTTCA CAGTAGTGCA GGCTACAGGG
    CAACCACGCA GTCAAGAGCA GATCGAGCTG CTGCAGGAGA TTCCCGCCAC CTTGAAGGAG
    CTTAAGGTTC AGAGCCACAG CATCTACAAT GGATTGGTGG GCTTTGCCTC TCCCCTTGTG
    ACAGAGAGAG GAAGTGACTG TATTAGTCCC ACTTCCACAG TCCAGACCAG CTTTGCCGCT
    GCAGTGCGCT GCAGTGGGGT GAAGACACAG CCTGACAGTA TGTCTCAGAA TGCACGCAAA
    GCTCTTCCTC GAAACTTTGG GACCCCGGCG GACACTCCTC CCAGCACACT GATGATCAAC
    AGCAAAGGAC TGGCCCCTAG CCCCAAAAGA GCCGTGCGAG ATCCCAAAAC TGGGAGAGCT
    GTGCAAGAGA GAAATTCATA TGCTGTTAGT GTGTGGAAAA GGGTGAAGGC CAAGCTGGAG
    GGGAGAGATG TGGATCCTAA CAGGAGAATG AGTGTCACTG AGCAGGTGGA TTATGTCATC
    AAAGAGGCAA CAAACGTGGA CAACCTGGCA CAGTTGTACG AGGGATGGAC GGCATGGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45857
    Clone ID Related Accession (Same CDS sequence) XM_009299454.3 , XM_009299454.2
    Accession Version XM_009299454.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase SMG1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299454.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG 
    AATGACTGGC AGCCCAGAAG CGACAGTCTG TCAGCCAGCC AGGACGGTGT GAAGTGCAGT
    GTCTCTAGAG ACCGCGGTTC AGAAGTTCTA TTTGAAATTC TGCCCTCTGA CCGGCCTGGC
    ACCCCACCAC TGCACAATGA CGCCTTCACT GCCACCGATG CACTCGAAGA GGGAGGTGGA
    TACGACGCTG ATGGAGGCCT CTCTGAAAAT GCTTATGGTT GGAAGTCGCT GGGGCAAGAG
    TTGAGAATTA ATGATGTGAC TACTGATATA ACCACTTTTC AGCATGGAAA CCGTGCTTTA
    GCTACAAAGG ACATGAGGAA ATCACAGGAC CGACCGTTGG CTCACTCTGA GGAGTCTCGT
    CTGGCCAATC TTCTGCGGAG AGCATCACGG GAAGATGACC GGGAACGCAG ACTGGCCACT
    ATGAAACAAA TGAAGGAGTT CATTGTGCAC TCTGAAAATA AAGTGGTCCT AGTGAAACAG
    TTGGATTCCA TTTTGAGCAC CCTGAATGAC ATTTTGAATG AGAGTAGTAA GCTCCTGCAG
    GAGCTCAGAC AGGAGGCCGC TTGGTGTCTG GGATTGCTCT GTGCCGCCCT CAGTTATGAA
    GCTGAGAGGA TCTTCAAGTG GATGTTCCTC AAGTTCAGTG TGTCTACAAA GGATGAGGTG
    AAGCTTCTGT ACCTTGTGGC AGTGCACAAA GCACTTGAAA CAGCTGGGGA GAAGAAGGCC
    TTTTCAGCTG TAATGCAGCT GGTGATGAGC AACCTTCAGT CTATATTGGA GAACTTGGAC
    ACACCGGAAC TATTGTGCCA AAGTGTAAAG TGTATTTTAC TGGTGGCCCG TTGTTATCCA
    CACATCTTCA GCACCAACTT CAGAGACACA GTGGATATCT TGGTAGGCTG GCATATAGAT
    CACACACAGA AGCACTCTCT TACTCAACAA GTATCTGGCT GGTTGCAGAG TTTGGAGCAA
    TTTTGGGTTG CAGATTTGGC CTTCTCGACC ACATTATTGG GTCAGTTTTT GGAAGATATG
    GAGGCATATG CTGAGGACCT GAGCCATGTT GTATCTGGAG AATCAGGAGA TGAGGACATT
    CCCCCTCCCA CTGTGTCCTT GCCTAAATTG GCAGCCCTAT TGAGAGTTTT TAGCACTGTT
    GTGCACAGCA TCGGAGAACG GTTTAACCCA ATCCGAGGCC CTCCAATTAC AGAAGCATAT
    GTCACTGATG TTTTGAATCG TGTTTTGGCT TGCGTCACCA CAGCCAAGCA GGTGTTCTTC
    TCTGAGGCGG TGCTGACAGC AGGAAATGAG TGTGTGTGTG TTCTGCTGGT GAGCATAGAC
    TTGGGCGGCC AGCTGATTGA TGCTGTAATC TCATACGGCC TAGACCAGCT GAACTGCTGC
    CAGAACTGCG GACCCGAATA CAGCCTGTCT GTTCTCACCC TGCTCACACT GGTTGTTGAC
    CAAATAAACA CCAAACTTCC GGCTTCTTTT GTGGAAAAGT TGTTGGCCCC AAAATCTCAT
    CTTTTGGAGC TGCGCTTTCA CAGAGAGAGA GAGGTGATGG CGGCAGCACA TGGAGTGTAT
    CATGCTGTGC TAAGCCTGAA GAACATCCCT ATCTTGGAGG CGGCATATAA ACTGGTCTTG
    GGCGAGATGG GCTGTGCACT GAACAGTTTG CTGAGCCCTC TGGGTTTGCC TGATGCCTGT
    CCTCACATCC AACATCCTGC TTTCAGCCAG CTGAACTTTA GCCCAGAGAG AGCTGAGTTT
    GTCCTCATCT TTAACCTCAG TGCTCTCACC ACCATTGGCA ACACCAAGAA CTCCCTCATC
    GGGATGTGGG CTCTGTCTCC TACTGTGTTT GCCCTGCTCA GTCAGAATCT GGTTATTGTT
    CATTCTGATT TGGCAGTTCA TCATCCTGCT GTCCAGTATG CAGTGCTCTA CACTCTCTAC
    TCTCACTGCA CCAGACATGA TCACTTCATC TCCAGCAGCC TGAGCTCCTC GTCCCCATCC
    CTTTTCGACG GTGCTGTCAT CAGCACAGTT ACCACGGCAA CCAAAAGGCA TTTCAGCACG
    CTCCTTAATT TGCTGGGTAT GCTGCTCAGC AAGGATCATC TCAACCCTGA GGCGAGGCGG
    CTGTTACTCA CCTGGTCATT GGAAGTAGCT CTGATGATGA AGAAATCTGA AACTTACGCT
    CCTCTCTTCT CGCTCCCATC CTTCCTCAAA TTCTGCAAAG GGTTACTGGC AAATTCCTTG
    AATGAGGACA CCACCATATG TCTGCAGGCA TGTAACAGTC TACAGGTGCT GTCATCTTCC
    TTGACTATGG AGCTTCTCCA GAGGTGTGTG GACGTGTGCC GAGTTCAGCT GGTTCACTCA
    GCAGTCCGAG TCCGGCAGGC CTTTGGGAAA TTGCTGAGAT CTGTTCCCAT GCACGTCGCA
    CTGAGCGCCC ATAGTCACTC TGAGATTAAG GAGATTTCTC TGGCCATCCG CCGACACATG
    AGCAAAGTGC CTAGCAACAC ATTCCACCCA CAGGACTTCT CTGACCTTAT TGGGTTTATT
    CTGTACGGAA CGGTCCATCG TGGGGGGAAG GAGCCCTGGT TGGAGCGTCT GTATCACAGC
    TGTCAGCGGC TGGAGAAGAA AGACTCTGCT ATGGTGCCTC GTGCCCTGCT GAAGACTGAG
    GCAGTGCTGT GGCAGTGGGC GGTGTGGGAA GCTGCTCAGT TTACTGTTCT CTCAAAGCTC
    CGTACACCTC TGGGAAGAGC CCAGGATACC TTCCAGACTA TAGAGGGTAT GATCCGTAGT
    CTGGCAGCTC ACAGTCTGAA CACAGAGCAG GAGCTGAGTC AGTGGAGCGG AGGAGAGAGT
    GATGAGGGTC ACCACACCAA CCAGCTCCGA CTGGCTCTTC TCCTGCAGTT CCTGGAGAAC
    CTGGAGAAAC TCATGTACAA CGCCTATGAA GGCTGTGCCA GTGCTCTCAC TGCACCACCA
    AAGGGCATTA GAACCTTTTT CTACACAAAC AGACAGACAT GCCAGGACTG GCTAACCAGA
    ATTCGTTTGG CCCTAATGAG GGTTGGCCTT CTCTCCGGTC AGCCAGCGGT CACAGTCCGT
    CATGGCTTTG ACCTGCTGAC TGAGATTAAA AACAGCAGTA CACAGGGTCC AGAAATGGAG
    GTTCCAGTGA CCATGCTGGT GGAGGCATTG TGCGAGTTGA GATGCCCAGA GGCTATTCAA
    GGTCTGGCAG CATGGAGTCT TGCCAACACT GGCAAAAGCA TGGGCTGGCT GAGCTCTGTG
    GCACTGCAGG CTGAGGGAAA GTTTGAGAAG GCAGCGTTAG AGTACCAAGA GCAGCTTTGT
    GCTGTCACAG GGATGGACTG CTCCATTAAA GTCTTTGATC GCTCACTTTT GAAACTCACT
    GGCACCAACA CCTCCAGTCC AAAACATACC AGCAGCGGAG AGGGAAGAAA AACTGTATTG
    CTGAAATCCA GCGAGTGTTC TCCAGAGGTT CTGAACTTTC TGGCTAATAA GGCCTGTGAG
    TGTTATGTGG CCCTGAGCGA TTGGGAGTCT GTGCAAGAAT GGCAGGCCAG CATGATGAAT
    CTCAAAAAGA ACAGCAGCAG TTCCAGTGTC AACCTCAAGA CAGACTTCAA CTACATCAGA
    GCTATGAGCA GGTTTGAGGA GGGAGATTTT ACAGAATGCC GTGCCCAGCT AGAGTTGCTC
    CCTGGGGATG ATTATGGCCT TCTCAACTCT ACAACTAAGG ACAAGATTGA CCTTAAGAGG
    CTTCTTCCTG CTGTGTTGAG TCCAGACCCA TCAGAGCTCC AGAAGGCCAT TGAGGTGCAG
    CTCCTCAGAA GTGCTGTGGG AGCCATCAGT GCGACCAATC ATGAGCAGGA CCAGAAAGTG
    GCATCATCAG ACACCCTGGT TAAATATCTG AAGCAGACGG GTCGCATCTG TCTGGGCCCC
    CTGCGCCTTT CCACTCTCAC CCTGTCAGAT TCCCTGCCCA CCCTTAGCAC TCTACAGCTG
    CACTGTGCCA ACTCTTTAGA GAACTCTCTT TGCAACCAAC ATCCTGAGGA CTGCCTTATT
    CCGCTCTTCA GTGAAGCTTT GACCACCTGC AAGCAGCAGG ATGTTCAACC ATGGCTGCAT
    GCTCTCCGAT ACACTACTTT TCAGCGGGAA TTCTTCCAAA AACTTAAAGG TTCATCTTCT
    CCAGTTGATT CTCATCTGAT GGAGCTTTGT CTGACTGCTG TAAAATTTGC TCGTAAACAA
    GGGAATATTG CTCTGGCCAC ACGCCTGCTG AGTCTATGCA GCAAGCCAGC TATGAGCGAC
    ACTGAAGGTC AGGACCTGGT TCAAAGCTTC AGGCAGCTGT CTCTAGAAGG CACTGTTGGG
    GAAAAGTGGG GACCAGAGCT GGAGATCGAG AAGGCTAAAG TTCTGTTCGC TGCTGGTCAG
    TCTGTGTCTG CTATGGAGAT GTTGAGCTCC TGTGCACTGT CATACTGTCA CTCTGGGAAG
    TGCGAACTTG CGGCCTGTCG CTCGGTGCTC ACTCTGTGCA AATGGCTTTT GGCTGACTGG
    AAAGATCTGA CTCCCCAGCT GAAGATGGTG GTGAAGAAGA ACTCTGGGTC TACCAGCCTC
    TCAACTCTCT CCAAAAACAT CTCTGGCTTG TTGGAGCTGC CGCTGGAAGA TCAGGGAATG
    CCACACATTA CAACAGAGAC TACAGTTAGC GTTGGTGTCG GGGAGCCAGA CTTTGTCCTT
    GGGCAACTTT ACCAGCTCTC AACTACTCAG GCTCCTGAAG TGGCCAAATC TTGGGCTGCT
    CTTGCCAGCT GGGCCTACAG ATGGGGCAGG AAGGTTGTGG ACAATGCCAG TCAAGGAGAA
    GGGGTTCCTC TGCTCCTCGG AGAGAAGAAA GAAATAGAGG AGTTGCTCCC AGCAGGCACC
    AGCGATGAGG ACAAGGAAAC CATTTTTGGC ATTTTGGGTC AAGCCATGTG TCGCCCAGCA
    GGAATTCAGG ATGAGGATAT GGCTCTGCAG AATGAAGAAG ATGATGAGGA CGACATGGTA
    GATGTAATTG GCCGACAGCT GTTGGGTGCC TGTCCCTGGC TTTCAGATGT AGAGGACACT
    GTGACCGATG GACTCATTGG GGTGTGGAGG AGAGTGGTTG ATCGGATCTT CAGCCTCTAC
    CGGGTCTCCT GCAGGGCTTA CTTCACTTTC CTCAAACTCA ATGCTGGACA GGTGCCTATA
    GATGAAGATG ATCCCAAGCT GCTCCTGAAC AACCAGAACA GCAAGCAGAG CAGCGATGAT
    GTCATTGTCA TGGCAACCCT CCGTCTGCTG CGCTTGTTGG TGAAGCACGC AGGAGAGTTG
    AGGGAAGGTC TGGAGCACGG TTTGGCATCC ACACCTACTG CTCCCTGGAG AGGTATTATC
    CCTCAGTTAT TCTCCAGACT TAATCACCCT GAAGCCTACA TCCGCCAGAG CATCTGCAGT
    CTGCTGTGTA GAGTGGCCCA AGATTCCCCA CACCTTATCC TTTATCCTGC CATTGTCGGA
    TCAATCTCTC TTGGTGGAGA GGCTCAGACG GCAGGTAATA AGCTGCCATC GTCCCTGCCA
    ACCCTGCTGG GGAACATGCA GGGAGAGGGT CTTTGTGGAG GTGAGAGCGA GACAGGCAGT
    GGGCCGACCT CTCAGGAGAG CAGCCGCGGG GAAGAGATGG TGATGTATTC CAGCGAGGAT
    CAAGCCATGA TGCAGGACTG CTACAGCAAG ATAGTAGACA AGCTTTCCTC TGCCAACCCT
    ACCATGGTGC TGCAGGTGCA GATGTTGGTG GGCGAGTTGA GGAGGGTCAC ACTGCTCTGG
    GACGAGCTCT GGCTGGGGGT CTTACAGCAG CAGCACATGC ACGTCCTGCG CAGGATTCAA
    CAGCTGGAGG ACGAGGTGAA GAGAGTTCAG AACAACAACA CTCTGCGCAA GGATGAGAAG
    GTGGCTATTA TGAGAGAGAA GCACTCTGCT CTGATGAAGC CTGTGGTTTT TGCGCTGGAT
    CATGTGCGCA GCATTACGGC TGCTCCAGCA GAGACGCCTC ACGAGGAATG GTTTCAGGAG
    ACATATGGAG ATGCCATCCA CAATGCCCTA GAGAGACTGA GGAGCCCTCT AAATCCAGCC
    AATCCTGCCA GCAGCTGGGT GCCCTTTAAA CAGATCATGC TGAGTTTACA GCAGAGGGCA
    CAGAAACGAG CCAGTTACCT TCTGCGTCTG GATGAGATCA GCCCACGTCT GACTGCTATG
    GCTAACACCG AGATGGCTCT GCCGGGAGAG GTGTCTGCTA CAGATGCAGT CACCATCCAG
    AGTGTGGGCA ACACCATCAC CATTCTGCCA ACTAAAACCA AACCCAAGAA GCTCTACTTT
    TTGGGATCGG ATGGGAGAAA CTATCCTTAT CTGTTCAAAG GATTGGAGGA TCTGCATTTG
    GATGAGAGGA TCATGCAGTT CTTGTCTATC GTCAACACCA TGTTCACTAA AGTGAATCAG
    CAGGAGAGCC CGAGGTTTCA AGCCCGTCAT TATTCTGTCA CACCTCTGGG TACCAGATCT
    GGCCTCATCC AGTGGGTGGA TGGAGCCACT CCACTGTTTG GCCTGTACAA ACGTTGGCAG
    CAGCGTGAAG CTGTGGTCCA AGCCCAGAAG GCACAGGACT CCTTCCAGCA GCCTCAGAAT
    CTTCCCATGG TCCCACGGCC CAGTGAGCTT TACTACAGTA AAATCAGTCC TGCCTTAAAG
    GCTGTCGGTC TCAGTTTGGA TGTGTCACGT CGTGATTGGC CGTTAAGTGT TATGAGAGAT
    GTTCTAAGAG AGCTGATGGA AGCCACGCCC CCTAACCTCT TGGCAAAGGA GCTCTGGTGT
    TCCTGCACCA CTCCTAGTGA GTGGTGGAGC GTCACTCAGA CTTACGCCAG ATCCACCGCT
    GTGATGTCAA TGGTGGGATA CATTATTGGC TTGGGGGATC GTCACCTTGA CAACGTGCTG
    ATTGACATGA CGACTGGCGA GGTGGTGCAC ATTGACTATA ACGTCTGTTT TGAGAAGGGA
    AAAAGCTTGA GAGTGCCTGA GAAAGTTCCT TTTCGCATGA CCCACAACAT CGAGACTGCT
    CTGGGGGTCA CAGGAGTTGA GGGAATCTTT AGACTGTCCT GTGAACAGGT GGTCCAGATA
    ATGCGGCGTG GGAGAGAGAC CCTCTTGACT CTTCTAGAGG CCTTTGTCTA TGACCCATTG
    GTGGACTGGA CAGCAGGCGG TGAGGTGGGC TTTGCAGGTG CTGTGTATGG AGGAGGTGGT
    CAGCAGGCTG AGAACAAGCA GAGCAAGAGA GAGATGGAGA GAGACATCAC ACGTAGCCTT
    TTCTCCTCCC GTGTGGCTGA AATTAAGGTG AACTGGTTCA AAAACCGTGA TGAAATGACT
    GGAGTGTTGC CACAGCTGGA GGAGGCAGTG GATGAGTACC TCAACCTGCA GGAGCAGCTC
    ACACAAGTGG AGAAGGTGCA GGGCAAGCTG TTGGAGGAGT TGGAGTTCCT GGAGGGAGCA
    GACACCAGAG CAGACCATCC CATCCACTCC CTGGAGCACA GATACTCAGA ACACACGCAG
    CTGCAGTCCA GACAGAGAAC TGTGCAGGAT GCCATTCAAG GGAAGTTGTC AGATCTGGAT
    CAATGGATCT CTCAGTATCA AGCTGCCTTT GCCAGTTTAG AAGCCACACA GCTTGCCAGC
    CTCCTCCAGG AGATCAGTAG CCCTATTGAT TTGGGTCCGC CCAGTTATGT ACCAGCAACG
    TCGTTCTTGC AGAATGCAGG TCAGGCACAT CTGATCAGCC AGTGTGAAGC GCTGGAGGCT
    GAAGTGAGTG CTCTGTTACA GCAGAGGCGC TCTCAGCTGC GAGGTTGTCT TGAGCACCTG
    CACAGTTATG CCACCGTGGC CCTGCTGTAC CCACGTGCCG TGCTGCACAG ACACAGAGCT
    CACACATGGA AACAGTGGAT GGAGGAGCTG GTGTGTGACA TGACGGTCGA TCACTGCCAG
    ACTATTTATC ACCAATACGA AATGCAGTTT GCACCCCAGC CCCCACCTGC CACGTGTCAG
    TTCCTCTCCA GCATCGAGAT GGCGCTGCAG CACCATGCAG CAGAAACCAA CACTCGTCTG
    CTGCGGCAGG TGGAGCGTCT GAAGGCAGAG GGCGCCAGTG TACCCGTGTG TGAGGAGCAG
    CTGCAGGAGA TCGAGCGCTG CATCAAAGTC TTCCTGCACG AGGACGCTGA GCTTGGCTCC
    TTCAGCCTGG CTGGCATCAT CGTCTCTGCC CTCTGTTCTC TCACCAGGCG TAATTTGGTG
    ATGGAGGGAG CTGCGGCCAG TGCTGGCGAG CAGCTGGTGG AGCTGACGTC TCGTGATGGA
    GCCTGGTTCC TAGAGGAGCT CTGCAGCATG AGTGGAAACA TTACATGTCT TGTGCAGCTG
    TTGCAGCAGT GCCAGCTTCT GTCACATGAC CTGGACATCC CTAGTCCTGC AGAAACTTCG
    CAAGTTGTCT ACCTGACCAA TGGTGTTTAC ACCTGTCTTC AGGAGCTGAA CACAAACTTC
    CGTCAGATCA TCTTCCCAGA GGCTCTGCGC TGCATGCTGA AAGGTGAAAA CACTTTAGAG
    ACCATGTTAG CAGAGCTGGA TGCGCTTATC GATCAGTGTG CAGATGGGGT TTCACTGCAG
    GGGCTTGGAG AGATTTTGAT GGCACACATT CGGAACGCCT CCATGGGTCT GGAAGAGGAT
    CCAGATGACC ACTATCTGGA CGTTACCAGG GTTTTGCGAG CCCAGTACTC TGAGCTGATT
    CAGCCCCGCA GCATGGAGAG CTCAGTGCAG GAAACGCCAA AGATGAGCGC TGGTCAGATG
    TTGCTGGTAG CTTTTGATGG CATGTTTGCC CAGTTGGAGA CAGCTTTTGG ACTTCTCATT
    GACAAGCTGA ACTCTATGGA TGTCCCTGCT GCCTGGAGAA AAGTGGATGT GATCCGGGAG
    TCGAGAGCCA CACAGGCTCA TTTCTTTGAT AATGTCCAGA CCAGGCAGGT CTTGGAGGAG
    ATTTTCTTCT TGAAGAGACT CCAGACCATC CGAGACTTCT TCCGTCTATG TGGTTCATTT
    GCACAGACAC TATCTGGCAC ATGCCCTACA CCCACTGATG ACCCTCCGCC ATCTAATGGC
    CCTGTTCCCA TAGTAAAGCC CTTGTATCGA GGCTCCACGG TGGTGAGCGA GGACCAGATG
    ACGCGGCCCA TCAAAGCCTT TACAGCAGAC TTTGTAAGGC AGATGCTGAT GGGGCTCCCC
    ACCCAGGCTC TGGGTCTAGC CATCTGCAGC TCTCTCAGTG CCCTGGGCAT GGATCTTATT
    GCTCAAGTGG AGGCCAAGGA CTTTGGAGCT GAGGGAAAAG TCTCTCTGGA TGACCTGTGC
    AAGAAAGCTG TAGAGCAGGG GGTGCAGGCA GGACGCCTGT CCCAGCTGCT ACTCAACAGG
    GCCACCGTGC TGGCCAGTTC CTATGACACT GCCTGGAAAA AACTTGACCT GGTCCGACGT
    CTGGAGGTCA GCATTGAAGC CTGTAAGGTC AGCCTGCAGA GGAATCAGCT TCACATCGCC
    ATGTTCCAGT GGCAACATGA GGACATTCTT GGTGCGAGGA CGCAGCCCAT GACAGTCAGT
    CCTCCACCAC GTTCCATTAT CCTCAGCAAC ATGAAAAAGA AGCTCTACAA ACTCAGTCAG
    GATGACGCTT CAATAGGAAG TGTCCAGGAG AAGTTGGCCA GTCTTGAAGG CAGTATAGAG
    CAGAGGCTGA AATGGGCCGG TGGTGCAAAT CCTGCACTCG CTCCAGTGCT GCAGGACTTT
    GAGGCCACTA TAGCAGAACG CCGTGCCTTG GTGATAAAGG AGAGTCAGCG TGCCAACCAG
    GTCACTTTCC TCTGCAGTAC CATTCTCAAC TTTGAAGGTC TGCGCACCCG CACACCCGAA
    GCTTTGAACA TGGATGCTGC CCTTTTTGAG CTTGTGAAAC GATGCCAGGC CACCTGCTCC
    TACGCTGCCC AGTTCAATAC CTCTGTTTCC TCGCTAGAAC TACAGCTGCT GCATAGACTG
    AGTCCTGCCA TGGATATGTC CATTGGTGGT CCTGAGTGGC TGGTGTATGC ACAGAATCAC
    CTGACCCAGG AGATGTCCAG TCAGAGAGCC ATGCAGGAGG AAAGAGAGCA GCAGCTGGAG
    AGCGTGACTG AGACCCTGCA GCTGCTGGTG GACTCCATTA AAGGCACCCT GTCCAATCAC
    AACCGCCAGC TCGCTGACGT TAAACACTTA CTGCGAGCAA TGGCAAAGGA TGAAGAGAAT
    GCCCTTGCAG AAGGAGAGGA AGTGACTTAT GATGGGAGTG TGCGTCAGTT CCTTTCTGAA
    TACAAGGCAT GGCAGGATAA TGTGCAGATA GTGCTGTTCA CAGTAGTGCA GGCTACAGGG
    CAACCACGCA GTCAAGAGCA GATCGAGCTG CTGCAGGAGA TTCCCGCCAC CTTGAAGGAG
    CTTAAGGTTC AGAGCCACAG CATCTACAAT GGATTGGTGG GCTTTGCCTC TCCCCTTGTG
    ACAGAGAGAG GAAGTGACTG TATTAGTCCC ACTTCCACAG TCCAGACCAG CTTTGCCGCT
    GCAGTGCGCT GCAGTGGGGT GAAGACACAG CCTGACAGTA TGTCTCAGAA TGCACGCAAA
    GCTCTTCCTC GAAACTTTGG GACCCCGGCG GACACTCCTC CCAGCACACT GATGATCAAC
    AGCAAAGGAC TGGCCCCTAG CCCCAAAAGA GCCGTGCGAG ATCCCAAAAC TGGGAGAGCT
    GTGCAAGAGA GAAATTCATA TGCTGTTAGT GTGTGGAAAA GGGTGAAGGC CAAGCTGGAG
    GGGAGAGATG TGGATCCTAA CAGGAGAATG AGTGTCACTG AGCAGGTGGA TTATGTCATC
    AAAGAGGCAA CAAACGTGGA CAACCTGGCA CAGTTGTACG AGGGATGGAC GGCATGGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297729.1
    CDS175..11097
    Translation

    Target ORF information:

    RefSeq Version XM_009299454.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio SMG1 phosphatidylinositol 3-kinase-related kinase (smg1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299454.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    ATGAGCCGCA AAGCCCTCGG GTCTCGGCTG AGCAGCGCCC ACAAACTGCA GCGAAACTGG 
    AATGACTGGC AGCCCAGAAG CGACAGTCTG TCAGCCAGCC AGGACGGTGT GAAGTGCAGT
    GTCTCTAGAG ACCGCGGTTC AGAAGTTCTA TTTGAAATTC TGCCCTCTGA CCGGCCTGGC
    ACCCCACCAC TGCACAATGA CGCCTTCACT GCCACCGATG CACTCGAAGA GGGAGGTGGA
    TACGACGCTG ATGGAGGCCT CTCTGAAAAT GCTTATGGTT GGAAGTCGCT GGGGCAAGAG
    TTGAGAATTA ATGATGTGAC TACTGATATA ACCACTTTTC AGCATGGAAA CCGTGCTTTA
    GCTACAAAGG ACATGAGGAA ATCACAGGAC CGACCGTTGG CTCACTCTGA GGAGTCTCGT
    CTGGCCAATC TTCTGCGGAG AGCATCACGG GAAGATGACC GGGAACGCAG ACTGGCCACT
    ATGAAACAAA TGAAGGAGTT CATTGTGCAC TCTGAAAATA AAGTGGTCCT AGTGAAACAG
    TTGGATTCCA TTTTGAGCAC CCTGAATGAC ATTTTGAATG AGAGTAGTAA GCTCCTGCAG
    GAGCTCAGAC AGGAGGCCGC TTGGTGTCTG GGATTGCTCT GTGCCGCCCT CAGTTATGAA
    GCTGAGAGGA TCTTCAAGTG GATGTTCCTC AAGTTCAGTG TGTCTACAAA GGATGAGGTG
    AAGCTTCTGT ACCTTGTGGC AGTGCACAAA GCACTTGAAA CAGCTGGGGA GAAGAAGGCC
    TTTTCAGCTG TAATGCAGCT GGTGATGAGC AACCTTCAGT CTATATTGGA GAACTTGGAC
    ACACCGGAAC TATTGTGCCA AAGTGTAAAG TGTATTTTAC TGGTGGCCCG TTGTTATCCA
    CACATCTTCA GCACCAACTT CAGAGACACA GTGGATATCT TGGTAGGCTG GCATATAGAT
    CACACACAGA AGCACTCTCT TACTCAACAA GTATCTGGCT GGTTGCAGAG TTTGGAGCAA
    TTTTGGGTTG CAGATTTGGC CTTCTCGACC ACATTATTGG GTCAGTTTTT GGAAGATATG
    GAGGCATATG CTGAGGACCT GAGCCATGTT GTATCTGGAG AATCAGGAGA TGAGGACATT
    CCCCCTCCCA CTGTGTCCTT GCCTAAATTG GCAGCCCTAT TGAGAGTTTT TAGCACTGTT
    GTGCACAGCA TCGGAGAACG GTTTAACCCA ATCCGAGGCC CTCCAATTAC AGAAGCATAT
    GTCACTGATG TTTTGAATCG TGTTTTGGCT TGCGTCACCA CAGCCAAGCA GGTGTTCTTC
    TCTGAGGCGG TGCTGACAGC AGGAAATGAG TGTGTGTGTG TTCTGCTGGT GAGCATAGAC
    TTGGGCGGCC AGCTGATTGA TGCTGTAATC TCATACGGCC TAGACCAGCT GAACTGCTGC
    CAGAACTGCG GACCCGAATA CAGCCTGTCT GTTCTCACCC TGCTCACACT GGTTGTTGAC
    CAAATAAACA CCAAACTTCC GGCTTCTTTT GTGGAAAAGT TGTTGGCCCC AAAATCTCAT
    CTTTTGGAGC TGCGCTTTCA CAGAGAGAGA GAGGTGATGG CGGCAGCACA TGGAGTGTAT
    CATGCTGTGC TAAGCCTGAA GAACATCCCT ATCTTGGAGG CGGCATATAA ACTGGTCTTG
    GGCGAGATGG GCTGTGCACT GAACAGTTTG CTGAGCCCTC TGGGTTTGCC TGATGCCTGT
    CCTCACATCC AACATCCTGC TTTCAGCCAG CTGAACTTTA GCCCAGAGAG AGCTGAGTTT
    GTCCTCATCT TTAACCTCAG TGCTCTCACC ACCATTGGCA ACACCAAGAA CTCCCTCATC
    GGGATGTGGG CTCTGTCTCC TACTGTGTTT GCCCTGCTCA GTCAGAATCT GGTTATTGTT
    CATTCTGATT TGGCAGTTCA TCATCCTGCT GTCCAGTATG CAGTGCTCTA CACTCTCTAC
    TCTCACTGCA CCAGACATGA TCACTTCATC TCCAGCAGCC TGAGCTCCTC GTCCCCATCC
    CTTTTCGACG GTGCTGTCAT CAGCACAGTT ACCACGGCAA CCAAAAGGCA TTTCAGCACG
    CTCCTTAATT TGCTGGGTAT GCTGCTCAGC AAGGATCATC TCAACCCTGA GGCGAGGCGG
    CTGTTACTCA CCTGGTCATT GGAAGTAGCT CTGATGATGA AGAAATCTGA AACTTACGCT
    CCTCTCTTCT CGCTCCCATC CTTCCTCAAA TTCTGCAAAG GGTTACTGGC AAATTCCTTG
    AATGAGGACA CCACCATATG TCTGCAGGCA TGTAACAGTC TACAGGTGCT GTCATCTTCC
    TTGACTATGG AGCTTCTCCA GAGGTGTGTG GACGTGTGCC GAGTTCAGCT GGTTCACTCA
    GCAGTCCGAG TCCGGCAGGC CTTTGGGAAA TTGCTGAGAT CTGTTCCCAT GCACGTCGCA
    CTGAGCGCCC ATAGTCACTC TGAGATTAAG GAGATTTCTC TGGCCATCCG CCGACACATG
    AGCAAAGTGC CTAGCAACAC ATTCCACCCA CAGGACTTCT CTGACCTTAT TGGGTTTATT
    CTGTACGGAA CGGTCCATCG TGGGGGGAAG GAGCCCTGGT TGGAGCGTCT GTATCACAGC
    TGTCAGCGGC TGGAGAAGAA AGACTCTGCT ATGGTGCCTC GTGCCCTGCT GAAGACTGAG
    GCAGTGCTGT GGCAGTGGGC GGTGTGGGAA GCTGCTCAGT TTACTGTTCT CTCAAAGCTC
    CGTACACCTC TGGGAAGAGC CCAGGATACC TTCCAGACTA TAGAGGGTAT GATCCGTAGT
    CTGGCAGCTC ACAGTCTGAA CACAGAGCAG GAGCTGAGTC AGTGGAGCGG AGGAGAGAGT
    GATGAGGGTC ACCACACCAA CCAGCTCCGA CTGGCTCTTC TCCTGCAGTT CCTGGAGAAC
    CTGGAGAAAC TCATGTACAA CGCCTATGAA GGCTGTGCCA GTGCTCTCAC TGCACCACCA
    AAGGGCATTA GAACCTTTTT CTACACAAAC AGACAGACAT GCCAGGACTG GCTAACCAGA
    ATTCGTTTGG CCCTAATGAG GGTTGGCCTT CTCTCCGGTC AGCCAGCGGT CACAGTCCGT
    CATGGCTTTG ACCTGCTGAC TGAGATTAAA AACAGCAGTA CACAGGGTCC AGAAATGGAG
    GTTCCAGTGA CCATGCTGGT GGAGGCATTG TGCGAGTTGA GATGCCCAGA GGCTATTCAA
    GGTCTGGCAG CATGGAGTCT TGCCAACACT GGCAAAAGCA TGGGCTGGCT GAGCTCTGTG
    GCACTGCAGG CTGAGGGAAA GTTTGAGAAG GCAGCGTTAG AGTACCAAGA GCAGCTTTGT
    GCTGTCACAG GGATGGACTG CTCCATTAAA GTCTTTGATC GCTCACTTTT GAAACTCACT
    GGCACCAACA CCTCCAGTCC AAAACATACC AGCAGCGGAG AGGGAAGAAA AACTGTATTG
    CTGAAATCCA GCGAGTGTTC TCCAGAGGTT CTGAACTTTC TGGCTAATAA GGCCTGTGAG
    TGTTATGTGG CCCTGAGCGA TTGGGAGTCT GTGCAAGAAT GGCAGGCCAG CATGATGAAT
    CTCAAAAAGA ACAGCAGCAG TTCCAGTGTC AACCTCAAGA CAGACTTCAA CTACATCAGA
    GCTATGAGCA GGTTTGAGGA GGGAGATTTT ACAGAATGCC GTGCCCAGCT AGAGTTGCTC
    CCTGGGGATG ATTATGGCCT TCTCAACTCT ACAACTAAGG ACAAGATTGA CCTTAAGAGG
    CTTCTTCCTG CTGTGTTGAG TCCAGACCCA TCAGAGCTCC AGAAGGCCAT TGAGGTGCAG
    CTCCTCAGAA GTGCTGTGGG AGCCATCAGT GCGACCAATC ATGAGCAGGA CCAGAAAGTG
    GCATCATCAG ACACCCTGGT TAAATATCTG AAGCAGACGG GTCGCATCTG TCTGGGCCCC
    CTGCGCCTTT CCACTCTCAC CCTGTCAGAT TCCCTGCCCA CCCTTAGCAC TCTACAGCTG
    CACTGTGCCA ACTCTTTAGA GAACTCTCTT TGCAACCAAC ATCCTGAGGA CTGCCTTATT
    CCGCTCTTCA GTGAAGCTTT GACCACCTGC AAGCAGCAGG ATGTTCAACC ATGGCTGCAT
    GCTCTCCGAT ACACTACTTT TCAGCGGGAA TTCTTCCAAA AACTTAAAGG TTCATCTTCT
    CCAGTTGATT CTCATCTGAT GGAGCTTTGT CTGACTGCTG TAAAATTTGC TCGTAAACAA
    GGGAATATTG CTCTGGCCAC ACGCCTGCTG AGTCTATGCA GCAAGCCAGC TATGAGCGAC
    ACTGAAGGTC AGGACCTGGT TCAAAGCTTC AGGCAGCTGT CTCTAGAAGG CACTGTTGGG
    GAAAAGTGGG GACCAGAGCT GGAGATCGAG AAGGCTAAAG TTCTGTTCGC TGCTGGTCAG
    TCTGTGTCTG CTATGGAGAT GTTGAGCTCC TGTGCACTGT CATACTGTCA CTCTGGGAAG
    TGCGAACTTG CGGCCTGTCG CTCGGTGCTC ACTCTGTGCA AATGGCTTTT GGCTGACTGG
    AAAGATCTGA CTCCCCAGCT GAAGATGGTG GTGAAGAAGA ACTCTGGGTC TACCAGCCTC
    TCAACTCTCT CCAAAAACAT CTCTGGCTTG TTGGAGCTGC CGCTGGAAGA TCAGGGAATG
    CCACACATTA CAACAGAGAC TACAGTTAGC GTTGGTGTCG GGGAGCCAGA CTTTGTCCTT
    GGGCAACTTT ACCAGCTCTC AACTACTCAG GCTCCTGAAG TGGCCAAATC TTGGGCTGCT
    CTTGCCAGCT GGGCCTACAG ATGGGGCAGG AAGGTTGTGG ACAATGCCAG TCAAGGAGAA
    GGGGTTCCTC TGCTCCTCGG AGAGAAGAAA GAAATAGAGG AGTTGCTCCC AGCAGGCACC
    AGCGATGAGG ACAAGGAAAC CATTTTTGGC ATTTTGGGTC AAGCCATGTG TCGCCCAGCA
    GGAATTCAGG ATGAGGATAT GGCTCTGCAG AATGAAGAAG ATGATGAGGA CGACATGGTA
    GATGTAATTG GCCGACAGCT GTTGGGTGCC TGTCCCTGGC TTTCAGATGT AGAGGACACT
    GTGACCGATG GACTCATTGG GGTGTGGAGG AGAGTGGTTG ATCGGATCTT CAGCCTCTAC
    CGGGTCTCCT GCAGGGCTTA CTTCACTTTC CTCAAACTCA ATGCTGGACA GGTGCCTATA
    GATGAAGATG ATCCCAAGCT GCTCCTGAAC AACCAGAACA GCAAGCAGAG CAGCGATGAT
    GTCATTGTCA TGGCAACCCT CCGTCTGCTG CGCTTGTTGG TGAAGCACGC AGGAGAGTTG
    AGGGAAGGTC TGGAGCACGG TTTGGCATCC ACACCTACTG CTCCCTGGAG AGGTATTATC
    CCTCAGTTAT TCTCCAGACT TAATCACCCT GAAGCCTACA TCCGCCAGAG CATCTGCAGT
    CTGCTGTGTA GAGTGGCCCA AGATTCCCCA CACCTTATCC TTTATCCTGC CATTGTCGGA
    TCAATCTCTC TTGGTGGAGA GGCTCAGACG GCAGGTAATA AGCTGCCATC GTCCCTGCCA
    ACCCTGCTGG GGAACATGCA GGGAGAGGGT CTTTGTGGAG GTGAGAGCGA GACAGGCAGT
    GGGCCGACCT CTCAGGAGAG CAGCCGCGGG GAAGAGATGG TGATGTATTC CAGCGAGGAT
    CAAGCCATGA TGCAGGACTG CTACAGCAAG ATAGTAGACA AGCTTTCCTC TGCCAACCCT
    ACCATGGTGC TGCAGGTGCA GATGTTGGTG GGCGAGTTGA GGAGGGTCAC ACTGCTCTGG
    GACGAGCTCT GGCTGGGGGT CTTACAGCAG CAGCACATGC ACGTCCTGCG CAGGATTCAA
    CAGCTGGAGG ACGAGGTGAA GAGAGTTCAG AACAACAACA CTCTGCGCAA GGATGAGAAG
    GTGGCTATTA TGAGAGAGAA GCACTCTGCT CTGATGAAGC CTGTGGTTTT TGCGCTGGAT
    CATGTGCGCA GCATTACGGC TGCTCCAGCA GAGACGCCTC ACGAGGAATG GTTTCAGGAG
    ACATATGGAG ATGCCATCCA CAATGCCCTA GAGAGACTGA GGAGCCCTCT AAATCCAGCC
    AATCCTGCCA GCAGCTGGGT GCCCTTTAAA CAGATCATGC TGAGTTTACA GCAGAGGGCA
    CAGAAACGAG CCAGTTACCT TCTGCGTCTG GATGAGATCA GCCCACGTCT GACTGCTATG
    GCTAACACCG AGATGGCTCT GCCGGGAGAG GTGTCTGCTA CAGATGCAGT CACCATCCAG
    AGTGTGGGCA ACACCATCAC CATTCTGCCA ACTAAAACCA AACCCAAGAA GCTCTACTTT
    TTGGGATCGG ATGGGAGAAA CTATCCTTAT CTGTTCAAAG GATTGGAGGA TCTGCATTTG
    GATGAGAGGA TCATGCAGTT CTTGTCTATC GTCAACACCA TGTTCACTAA AGTGAATCAG
    CAGGAGAGCC CGAGGTTTCA AGCCCGTCAT TATTCTGTCA CACCTCTGGG TACCAGATCT
    GGCCTCATCC AGTGGGTGGA TGGAGCCACT CCACTGTTTG GCCTGTACAA ACGTTGGCAG
    CAGCGTGAAG CTGTGGTCCA AGCCCAGAAG GCACAGGACT CCTTCCAGCA GCCTCAGAAT
    CTTCCCATGG TCCCACGGCC CAGTGAGCTT TACTACAGTA AAATCAGTCC TGCCTTAAAG
    GCTGTCGGTC TCAGTTTGGA TGTGTCACGT CGTGATTGGC CGTTAAGTGT TATGAGAGAT
    GTTCTAAGAG AGCTGATGGA AGCCACGCCC CCTAACCTCT TGGCAAAGGA GCTCTGGTGT
    TCCTGCACCA CTCCTAGTGA GTGGTGGAGC GTCACTCAGA CTTACGCCAG ATCCACCGCT
    GTGATGTCAA TGGTGGGATA CATTATTGGC TTGGGGGATC GTCACCTTGA CAACGTGCTG
    ATTGACATGA CGACTGGCGA GGTGGTGCAC ATTGACTATA ACGTCTGTTT TGAGAAGGGA
    AAAAGCTTGA GAGTGCCTGA GAAAGTTCCT TTTCGCATGA CCCACAACAT CGAGACTGCT
    CTGGGGGTCA CAGGAGTTGA GGGAATCTTT AGACTGTCCT GTGAACAGGT GGTCCAGATA
    ATGCGGCGTG GGAGAGAGAC CCTCTTGACT CTTCTAGAGG CCTTTGTCTA TGACCCATTG
    GTGGACTGGA CAGCAGGCGG TGAGGTGGGC TTTGCAGGTG CTGTGTATGG AGGAGGTGGT
    CAGCAGGCTG AGAACAAGCA GAGCAAGAGA GAGATGGAGA GAGACATCAC ACGTAGCCTT
    TTCTCCTCCC GTGTGGCTGA AATTAAGGTG AACTGGTTCA AAAACCGTGA TGAAATGACT
    GGAGTGTTGC CACAGCTGGA GGAGGCAGTG GATGAGTACC TCAACCTGCA GGAGCAGCTC
    ACACAAGTGG AGAAGGTGCA GGGCAAGCTG TTGGAGGAGT TGGAGTTCCT GGAGGGAGCA
    GACACCAGAG CAGACCATCC CATCCACTCC CTGGAGCACA GATACTCAGA ACACACGCAG
    CTGCAGTCCA GACAGAGAAC TGTGCAGGAT GCCATTCAAG GGAAGTTGTC AGATCTGGAT
    CAATGGATCT CTCAGTATCA AGCTGCCTTT GCCAGTTTAG AAGCCACACA GCTTGCCAGC
    CTCCTCCAGG AGATCAGTAG CCCTATTGAT TTGGGTCCGC CCAGTTATGT ACCAGCAACG
    TCGTTCTTGC AGAATGCAGG TCAGGCACAT CTGATCAGCC AGTGTGAAGC GCTGGAGGCT
    GAAGTGAGTG CTCTGTTACA GCAGAGGCGC TCTCAGCTGC GAGGTTGTCT TGAGCACCTG
    CACAGTTATG CCACCGTGGC CCTGCTGTAC CCACGTGCCG TGCTGCACAG ACACAGAGCT
    CACACATGGA AACAGTGGAT GGAGGAGCTG GTGTGTGACA TGACGGTCGA TCACTGCCAG
    ACTATTTATC ACCAATACGA AATGCAGTTT GCACCCCAGC CCCCACCTGC CACGTGTCAG
    TTCCTCTCCA GCATCGAGAT GGCGCTGCAG CACCATGCAG CAGAAACCAA CACTCGTCTG
    CTGCGGCAGG TGGAGCGTCT GAAGGCAGAG GGCGCCAGTG TACCCGTGTG TGAGGAGCAG
    CTGCAGGAGA TCGAGCGCTG CATCAAAGTC TTCCTGCACG AGGACGCTGA GCTTGGCTCC
    TTCAGCCTGG CTGGCATCAT CGTCTCTGCC CTCTGTTCTC TCACCAGGCG TAATTTGGTG
    ATGGAGGGAG CTGCGGCCAG TGCTGGCGAG CAGCTGGTGG AGCTGACGTC TCGTGATGGA
    GCCTGGTTCC TAGAGGAGCT CTGCAGCATG AGTGGAAACA TTACATGTCT TGTGCAGCTG
    TTGCAGCAGT GCCAGCTTCT GTCACATGAC CTGGACATCC CTAGTCCTGC AGAAACTTCG
    CAAGTTGTCT ACCTGACCAA TGGTGTTTAC ACCTGTCTTC AGGAGCTGAA CACAAACTTC
    CGTCAGATCA TCTTCCCAGA GGCTCTGCGC TGCATGCTGA AAGGTGAAAA CACTTTAGAG
    ACCATGTTAG CAGAGCTGGA TGCGCTTATC GATCAGTGTG CAGATGGGGT TTCACTGCAG
    GGGCTTGGAG AGATTTTGAT GGCACACATT CGGAACGCCT CCATGGGTCT GGAAGAGGAT
    CCAGATGACC ACTATCTGGA CGTTACCAGG GTTTTGCGAG CCCAGTACTC TGAGCTGATT
    CAGCCCCGCA GCATGGAGAG CTCAGTGCAG GAAACGCCAA AGATGAGCGC TGGTCAGATG
    TTGCTGGTAG CTTTTGATGG CATGTTTGCC CAGTTGGAGA CAGCTTTTGG ACTTCTCATT
    GACAAGCTGA ACTCTATGGA TGTCCCTGCT GCCTGGAGAA AAGTGGATGT GATCCGGGAG
    TCGAGAGCCA CACAGGCTCA TTTCTTTGAT AATGTCCAGA CCAGGCAGGT CTTGGAGGAG
    ATTTTCTTCT TGAAGAGACT CCAGACCATC CGAGACTTCT TCCGTCTATG TGGTTCATTT
    GCACAGACAC TATCTGGCAC ATGCCCTACA CCCACTGATG ACCCTCCGCC ATCTAATGGC
    CCTGTTCCCA TAGTAAAGCC CTTGTATCGA GGCTCCACGG TGGTGAGCGA GGACCAGATG
    ACGCGGCCCA TCAAAGCCTT TACAGCAGAC TTTGTAAGGC AGATGCTGAT GGGGCTCCCC
    ACCCAGGCTC TGGGTCTAGC CATCTGCAGC TCTCTCAGTG CCCTGGGCAT GGATCTTATT
    GCTCAAGTGG AGGCCAAGGA CTTTGGAGCT GAGGGAAAAG TCTCTCTGGA TGACCTGTGC
    AAGAAAGCTG TAGAGCAGGG GGTGCAGGCA GGACGCCTGT CCCAGCTGCT ACTCAACAGG
    GCCACCGTGC TGGCCAGTTC CTATGACACT GCCTGGAAAA AACTTGACCT GGTCCGACGT
    CTGGAGGTCA GCATTGAAGC CTGTAAGGTC AGCCTGCAGA GGAATCAGCT TCACATCGCC
    ATGTTCCAGT GGCAACATGA GGACATTCTT GGTGCGAGGA CGCAGCCCAT GACAGTCAGT
    CCTCCACCAC GTTCCATTAT CCTCAGCAAC ATGAAAAAGA AGCTCTACAA ACTCAGTCAG
    GATGACGCTT CAATAGGAAG TGTCCAGGAG AAGTTGGCCA GTCTTGAAGG CAGTATAGAG
    CAGAGGCTGA AATGGGCCGG TGGTGCAAAT CCTGCACTCG CTCCAGTGCT GCAGGACTTT
    GAGGCCACTA TAGCAGAACG CCGTGCCTTG GTGATAAAGG AGAGTCAGCG TGCCAACCAG
    GTCACTTTCC TCTGCAGTAC CATTCTCAAC TTTGAAGGTC TGCGCACCCG CACACCCGAA
    GCTTTGAACA TGGATGCTGC CCTTTTTGAG CTTGTGAAAC GATGCCAGGC CACCTGCTCC
    TACGCTGCCC AGTTCAATAC CTCTGTTTCC TCGCTAGAAC TACAGCTGCT GCATAGACTG
    AGTCCTGCCA TGGATATGTC CATTGGTGGT CCTGAGTGGC TGGTGTATGC ACAGAATCAC
    CTGACCCAGG AGATGTCCAG TCAGAGAGCC ATGCAGGAGG AAAGAGAGCA GCAGCTGGAG
    AGCGTGACTG AGACCCTGCA GCTGCTGGTG GACTCCATTA AAGGCACCCT GTCCAATCAC
    AACCGCCAGC TCGCTGACGT TAAACACTTA CTGCGAGCAA TGGCAAAGGA TGAAGAGAAT
    GCCCTTGCAG AAGGAGAGGA AGTGACTTAT GATGGGAGTG TGCGTCAGTT CCTTTCTGAA
    TACAAGGCAT GGCAGGATAA TGTGCAGATA GTGCTGTTCA CAGTAGTGCA GGCTACAGGG
    CAACCACGCA GTCAAGAGCA GATCGAGCTG CTGCAGGAGA TTCCCGCCAC CTTGAAGGAG
    CTTAAGGTTC AGAGCCACAG CATCTACAAT GGATTGGTGG GCTTTGCCTC TCCCCTTGTG
    ACAGAGAGAG GAAGTGACTG TATTAGTCCC ACTTCCACAG TCCAGACCAG CTTTGCCGCT
    GCAGTGCGCT GCAGTGGGGT GAAGACACAG CCTGACAGTA TGTCTCAGAA TGCACGCAAA
    GCTCTTCCTC GAAACTTTGG GACCCCGGCG GACACTCCTC CCAGCACACT GATGATCAAC
    AGCAAAGGAC TGGCCCCTAG CCCCAAAAGA GCCGTGCGAG ATCCCAAAAC TGGGAGAGCT
    GTGCAAGAGA GAAATTCATA TGCTGTTAGT GTGTGGAAAA GGGTGAAGGC CAAGCTGGAG
    GGGAGAGATG TGGATCCTAA CAGGAGAATG AGTGTCACTG AGCAGGTGGA TTATGTCATC
    AAAGAGGCAA CAAACGTGGA CAACCTGGCA CAGTTGTACG AGGGATGGAC GGCATGGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.