wrn cDNA ORF clone, Danio rerio(zebrafish)

The following wrn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wrn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa145058 XM_009303971.3
Latest version!
Danio rerio Werner syndrome (wrn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa45771 XM_009304279.2
Latest version!
Danio rerio Werner syndrome (wrn), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa45770 XM_021479248.1
Latest version!
Danio rerio Werner syndrome (wrn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa45770 XM_009303971.2
Latest version!
Danio rerio Werner syndrome (wrn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa145058
    Clone ID Related Accession (Same CDS sequence) XM_009303971.3
    Accession Version XM_009303971.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Werner syndrome ATP-dependent helicase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394765.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303971.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGTGACA GGACTCTACC TAAATGGATG AATAACAAAC CTCAGGATGA ACACACATCA 
    GGAACTTATA AAAAGAATGT TTTGGAGGAT CATCTACCGT ATCTGGAATT TGGTGGAACA
    GTCGTATACA GCCAGGAAAG ACACGACTGT TCTTTTTTAT CAGAGGATTT AAGATCTGCT
    TTGTCGTCTG GCTCAGCGGT GGGATTTGAT CTGGAATGGC CACCAAGCTT CACCAAGGGG
    AAGACAAAGA AAGTGGCAAT GGTGCAGCTG TGTGCATCTG AGGACAAATG CTATTTGTTT
    CACATCTCCT CCATGTCAGG GTTTCCTCCT GGCCTGAAGA TGTTTCTAGA GGATGAGAAC
    ATCATGAAAG TTGGCGTGGG AATTGAGGGT GACAAATGGA AGTTATTGTC TGATTATGAC
    ATCAAGTTAA AGAATATCGT TGATCTCTCT GATCTGGCAA ATGAGAAGCT GAGATGTTGT
    GAGAAGTGGA GTTTGGATGG CCTTGTGAAG CACTTGTTGA AGAAGCAGCT CTTTAAGGAC
    AAACTGGTAC GCTGTAGTCA CTGGGATGAT TTTAGTCTGA CAGAAGACCA GAAGAGATAT
    GCTGCAACAG ATGCTTACGC TGGATTGCTC ATTTATCAGA AACTACAGGA AATGATATCT
    GGTTGCTCAG GATCCTCAGC ACCCAGTTTG AAAGAGAGAG TGTTAAAAAT TGCTTGTGAT
    CTAAAAGATC TGGCTGATCA TATTCCCAAT GAACTTGACT GCACAGACGG TGCTGGGAGA
    CTAGTGGATG ATCTGTCTCA AACTCTTACA TCTCTAAGAT GTGTTTTGTC AAATGCCAAC
    AAGCCTACTG AACAATTAGT TGAAGAAAAG CCTTCTCCAG GACTTATTCT CGACGACACC
    AGTTCTCACC ATGAGGGCGT CCCCACAACT GAAGAGCAGA GCCACAGTCA GTGTGTGGAA
    AACCCTGGCA GACCTCCCCA GAGAGAGTGT GCGATGTCTC TGGATATCTC CGAGTACGAG
    CTGCAGATGC TGGAGGAACA GGCCAGACAG GAGCAGCTGG AGGAGGAGTG TATCTTAGCT
    TTCCAGACTC AACACGCATC GGGTCCCCAG GATTTAAATG CGACAGAGAG CGATGAAGAG
    TTTGATGATG AAATGATGAA GTGTGCAGAT GACATAGAAA AAATAAACAA TGCACAGATG
    CCTCAGTCTG GTGCCGAAGA CGAAGATGAA GATGTTGAGG AGAACGAGGA AGAAGGGCTA
    GATTCAAGCA TTCCACAGCC AGTTCCTGAA CAAATCAAAT GTCTGAAAAT GTTCTTTGGA
    CATCACAGTT TCAAACCTGT TCAGTGGAAA GTGATTCAGT CTATTCTCAC AGAGAGGAGA
    GATAATCTTG TCGTCATGGC AACAGGTTAT GGGAAGAGTT TGTGCTTCCA GTTTCCTCCT
    GTTTACTGCC AGACAATCAG TGTGGTCATT TCACCACTCA TCGCCCTCAT GGAGGATCAA
    GTGCTTCAGC TCACGATGTC AAACATTCCT GCTTGTTTTC TTGGATCTGC TCAGACCAAA
    AACCTAAATG AGCAAGTGAA GAGGGGGGAT TTCAGAGTGG TGTACATGAC CCCTGAGTTC
    TGCTCGGGGA ACATTCCTCT GCTAGTGCAG CTCGACAGGA CTGTTGGCTT ATCACTGATT
    GCGGTTGATG AGGCTCATTG CATTTCTCAG TGGGGTCATG ATTTCAGAAG TGCGTATCGG
    GAGTTGGGCA AACTCAAGAA AAATCTCCCC ACTGTCCCTT TTTTGGCACT CACGGCTACC
    GCAAGTCCAT CTATCCGTGA CGACATCATT AAGAGTCTTC ATCTGATCAA TCCCATGATT
    ACCTGTACTT CATTTGACCG ACCAAACCTT TACTTGGATG TCAATCGCAA GTCCGGGGAC
    GTCATCCAAG ACCTCAAACG ATTTCTTGTT AAAAAGAAAG GGGGTGGTTA TGAGTTTGAG
    GGCTCTGCCA TTGTGTACTG TCCATCAAAG AAAGAGGCAG AGCGTGTGAC AACAGCACTA
    TTCAAGCTGG ACATCCCATG TGGAGTTTAT CACGCTGGTC TGAGCATCAA ACAGAGGAGA
    GAGACTCAGC ACCAGTTCAT GAGGGATGAG ATTCAGTGTG TGGTGGCCAC AGTGGCGTTT
    GGGATGGGCA TCAATAAATC TGACATCAGG AAGGTCATCC ATTATGGGGC TCCCAAAGAA
    ATGGAGTCCT ACTATCAGGA GATTGGCAGG GCTGGTAGAG ACGGGCTGCC CAGTGCCTGT
    CACGTCTTAT GGATGCCCGG AGATATGGCG CTGAATAAAT TCATTCTCAA CCAATCCAAA
    AGTGAGCGCT TCAGGAGTTA CAAAATAGAC ATGATGGCAA AAATGGAAAA GTATCTGAAT
    TCAACCAAAT GTAGAAGAAA GTTGATTTTG TGTCATTTTG AAGACAAGCG TCTCAGGAAA
    GTGACGTCTG GAATTCTGGG AAGCAGTCAA TGTTGCGATA ACTGCCGGTC AGGTGTGAAT
    GAAGAGGATC TAGAGGTGCT GCAGCAGGAC TTTGGCTTGT GTGCATATCA GTTGATGAAG
    GCCATCAGTG TTATGGAGGA GAGGTTTGGC ATCACAGCGC CCATCCTCTT CCTGCGTGGT
    TCATGTTCTC AGCGGGTTCC AGAACGCTTT CGGAAGCACT CTCTCTTTGG TATTGGCAGG
    GACGTCTCAG AAACTTGGTG GAAAGCTCTT GGCCGAGAAC TTATTGCTGA GAAATATCTG
    ATGGAAGTCA CAGGCCACAG CAAGTTCACC ACTCTCTGTA AAATCACAAC TAAGGGAAGA
    TCATGGCTGA GTAAAGCTGA AGATGAGAAG CACAGACAGC TTTTGCTGCA GCCCAACAGA
    GATCTGTTCA GCAGAGTGTG TGCTCCGCGG AGAAAAGCAG ATCCAGCACA AGTTGAACCT
    ACAGTTTCTA ACCTGCAGTC ACCTCACATA CGGCCCTCTC TTGTTACATT TCAGAGGACA
    AAGCTTGGTT CTGAATCCCC TCAGATTGTG AAGAAAGGAG ATTTGCCTTC TAGGTCAGCA
    GCCAGACAAA CACAGATTCA AACTGATGTC AGAGCCCAAA CTCCAGGAGC CTCTGCTAAA
    GAAATTCAGC TACAGTCTGA GCTGTATGGT AAGCTGGTGT CTGAGCGTCA GAAGTTAGCT
    AGTATCAGAG ACATCCCACC AGCTCTGCTG GCCACCAATA AAATTCTTCT GGACATGTCA
    AAATTAAGGC CCTGCACGAT GTCCAGCTTG AAACAGGTGG ATGGAGTTTC AGAAGCAAAA
    GCAGCCATGT TAACACCTCT CATAGATATC ATCGCCAAAT TCTGCCAGAC ACACGGATTA
    CAGGTGGATG TTTCCTCCAA CTCTGCTTCA TCTGTCCAAA CACACTCCTG TCGTGAATCA
    GTTGTAGGAT CTGTGGCTCA GTGTCTGCCG GACAGCATCT CCATCACATA CAGCCTCTTC
    CAGACAGAGC ACAAGAGCTT GAGGCAGGTA TCAGATGCTC GCTCTCTGCC CATAGCGGTG
    GTGGAGTCTC ATCTTCTGCA AGCTCAGAAA ATGGGCTGCG CTGTGGACAC TGACAGAGCA
    GGACTCTCAA CATCCATCAT GAACACCATC AGAAAAAACA CAGCCGGCTC AGATTTGTGT
    GATTTTAAAG CAGTCCGTTC TCGTGTCCCT GAAGATATTA GTAACTTTCT CCTGAGGTTG
    GCCATTTATG AACTCCAGAA AGAAGCTTCA TCCACAACAC GTCATGAAAT CAGCTGGATT
    GAGCCACTGG ACAAATCAAC TACTGCAACC AGACTAGCAG AAAAACCTGT GTGTGAAGAG
    CGACAGCAAG AGCCAGACGC CATAGACACC AGTGAAGATG ATATCTTTAT CAGTATTCCC
    ATGCCTCAGG AAGAACCCTG CTTGGGGAGT GATGTACCTC TGAAGTCACA TGAGCCAGTT
    CCTCAGAGCA GTGGAATGGA GCTGGCTTCC TGGAACCAGG ATAACCTGGA TAAGGACACA
    CAAGATCTCT TCAGCGATTC CCCTGTGAAG ACTGTGAGTC AGCCTGCAAA GAGAAAACTG
    CCTGACTGGA CTGCAGGACC TACAACATCA GTAACGACTA CATCGGAAAA GAGAACCAAA
    AAGAAGAAAG GACTTTTCAG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302246.2
    CDS248..4411
    Translation

    Target ORF information:

    RefSeq Version XM_009303971.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Werner syndrome (wrn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303971.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGTGACA GGACTCTACC TAAATGGATG AATAACAAAC CTCAGGATGA ACACACATCA 
    GGAACTTATA AAAAGAATGT TTTGGAGGAT CATCTACCGT ATCTGGAATT TGGTGGAACA
    GTCGTATACA GCCAGGAAAG ACACGACTGT TCTTTTTTAT CAGAGGATTT AAGATCTGCT
    TTGTCGTCTG GCTCAGCGGT GGGATTTGAT CTGGAATGGC CACCAAGCTT CACCAAGGGG
    AAGACAAAGA AAGTGGCAAT GGTGCAGCTG TGTGCATCTG AGGACAAATG CTATTTGTTT
    CACATCTCCT CCATGTCAGG GTTTCCTCCT GGCCTGAAGA TGTTTCTAGA GGATGAGAAC
    ATCATGAAAG TTGGCGTGGG AATTGAGGGT GACAAATGGA AGTTATTGTC TGATTATGAC
    ATCAAGTTAA AGAATATCGT TGATCTCTCT GATCTGGCAA ATGAGAAGCT GAGATGTTGT
    GAGAAGTGGA GTTTGGATGG CCTTGTGAAG CACTTGTTGA AGAAGCAGCT CTTTAAGGAC
    AAACTGGTAC GCTGTAGTCA CTGGGATGAT TTTAGTCTGA CAGAAGACCA GAAGAGATAT
    GCTGCAACAG ATGCTTACGC TGGATTGCTC ATTTATCAGA AACTACAGGA AATGATATCT
    GGTTGCTCAG GATCCTCAGC ACCCAGTTTG AAAGAGAGAG TGTTAAAAAT TGCTTGTGAT
    CTAAAAGATC TGGCTGATCA TATTCCCAAT GAACTTGACT GCACAGACGG TGCTGGGAGA
    CTAGTGGATG ATCTGTCTCA AACTCTTACA TCTCTAAGAT GTGTTTTGTC AAATGCCAAC
    AAGCCTACTG AACAATTAGT TGAAGAAAAG CCTTCTCCAG GACTTATTCT CGACGACACC
    AGTTCTCACC ATGAGGGCGT CCCCACAACT GAAGAGCAGA GCCACAGTCA GTGTGTGGAA
    AACCCTGGCA GACCTCCCCA GAGAGAGTGT GCGATGTCTC TGGATATCTC CGAGTACGAG
    CTGCAGATGC TGGAGGAACA GGCCAGACAG GAGCAGCTGG AGGAGGAGTG TATCTTAGCT
    TTCCAGACTC AACACGCATC GGGTCCCCAG GATTTAAATG CGACAGAGAG CGATGAAGAG
    TTTGATGATG AAATGATGAA GTGTGCAGAT GACATAGAAA AAATAAACAA TGCACAGATG
    CCTCAGTCTG GTGCCGAAGA CGAAGATGAA GATGTTGAGG AGAACGAGGA AGAAGGGCTA
    GATTCAAGCA TTCCACAGCC AGTTCCTGAA CAAATCAAAT GTCTGAAAAT GTTCTTTGGA
    CATCACAGTT TCAAACCTGT TCAGTGGAAA GTGATTCAGT CTATTCTCAC AGAGAGGAGA
    GATAATCTTG TCGTCATGGC AACAGGTTAT GGGAAGAGTT TGTGCTTCCA GTTTCCTCCT
    GTTTACTGCC AGACAATCAG TGTGGTCATT TCACCACTCA TCGCCCTCAT GGAGGATCAA
    GTGCTTCAGC TCACGATGTC AAACATTCCT GCTTGTTTTC TTGGATCTGC TCAGACCAAA
    AACCTAAATG AGCAAGTGAA GAGGGGGGAT TTCAGAGTGG TGTACATGAC CCCTGAGTTC
    TGCTCGGGGA ACATTCCTCT GCTAGTGCAG CTCGACAGGA CTGTTGGCTT ATCACTGATT
    GCGGTTGATG AGGCTCATTG CATTTCTCAG TGGGGTCATG ATTTCAGAAG TGCGTATCGG
    GAGTTGGGCA AACTCAAGAA AAATCTCCCC ACTGTCCCTT TTTTGGCACT CACGGCTACC
    GCAAGTCCAT CTATCCGTGA CGACATCATT AAGAGTCTTC ATCTGATCAA TCCCATGATT
    ACCTGTACTT CATTTGACCG ACCAAACCTT TACTTGGATG TCAATCGCAA GTCCGGGGAC
    GTCATCCAAG ACCTCAAACG ATTTCTTGTT AAAAAGAAAG GGGGTGGTTA TGAGTTTGAG
    GGCTCTGCCA TTGTGTACTG TCCATCAAAG AAAGAGGCAG AGCGTGTGAC AACAGCACTA
    TTCAAGCTGG ACATCCCATG TGGAGTTTAT CACGCTGGTC TGAGCATCAA ACAGAGGAGA
    GAGACTCAGC ACCAGTTCAT GAGGGATGAG ATTCAGTGTG TGGTGGCCAC AGTGGCGTTT
    GGGATGGGCA TCAATAAATC TGACATCAGG AAGGTCATCC ATTATGGGGC TCCCAAAGAA
    ATGGAGTCCT ACTATCAGGA GATTGGCAGG GCTGGTAGAG ACGGGCTGCC CAGTGCCTGT
    CACGTCTTAT GGATGCCCGG AGATATGGCG CTGAATAAAT TCATTCTCAA CCAATCCAAA
    AGTGAGCGCT TCAGGAGTTA CAAAATAGAC ATGATGGCAA AAATGGAAAA GTATCTGAAT
    TCAACCAAAT GTAGAAGAAA GTTGATTTTG TGTCATTTTG AAGACAAGCG TCTCAGGAAA
    GTGACGTCTG GAATTCTGGG AAGCAGTCAA TGTTGCGATA ACTGCCGGTC AGGTGTGAAT
    GAAGAGGATC TAGAGGTGCT GCAGCAGGAC TTTGGCTTGT GTGCATATCA GTTGATGAAG
    GCCATCAGTG TTATGGAGGA GAGGTTTGGC ATCACAGCGC CCATCCTCTT CCTGCGTGGT
    TCATGTTCTC AGCGGGTTCC AGAACGCTTT CGGAAGCACT CTCTCTTTGG TATTGGCAGG
    GACGTCTCAG AAACTTGGTG GAAAGCTCTT GGCCGAGAAC TTATTGCTGA GAAATATCTG
    ATGGAAGTCA CAGGCCACAG CAAGTTCACC ACTCTCTGTA AAATCACAAC TAAGGGAAGA
    TCATGGCTGA GTAAAGCTGA AGATGAGAAG CACAGACAGC TTTTGCTGCA GCCCAACAGA
    GATCTGTTCA GCAGAGTGTG TGCTCCGCGG AGAAAAGCAG ATCCAGCACA AGTTGAACCT
    ACAGTTTCTA ACCTGCAGTC ACCTCACATA CGGCCCTCTC TTGTTACATT TCAGAGGACA
    AAGCTTGGTT CTGAATCCCC TCAGATTGTG AAGAAAGGAG ATTTGCCTTC TAGGTCAGCA
    GCCAGACAAA CACAGATTCA AACTGATGTC AGAGCCCAAA CTCCAGGAGC CTCTGCTAAA
    GAAATTCAGC TACAGTCTGA GCTGTATGGT AAGCTGGTGT CTGAGCGTCA GAAGTTAGCT
    AGTATCAGAG ACATCCCACC AGCTCTGCTG GCCACCAATA AAATTCTTCT GGACATGTCA
    AAATTAAGGC CCTGCACGAT GTCCAGCTTG AAACAGGTGG ATGGAGTTTC AGAAGCAAAA
    GCAGCCATGT TAACACCTCT CATAGATATC ATCGCCAAAT TCTGCCAGAC ACACGGATTA
    CAGGTGGATG TTTCCTCCAA CTCTGCTTCA TCTGTCCAAA CACACTCCTG TCGTGAATCA
    GTTGTAGGAT CTGTGGCTCA GTGTCTGCCG GACAGCATCT CCATCACATA CAGCCTCTTC
    CAGACAGAGC ACAAGAGCTT GAGGCAGGTA TCAGATGCTC GCTCTCTGCC CATAGCGGTG
    GTGGAGTCTC ATCTTCTGCA AGCTCAGAAA ATGGGCTGCG CTGTGGACAC TGACAGAGCA
    GGACTCTCAA CATCCATCAT GAACACCATC AGAAAAAACA CAGCCGGCTC AGATTTGTGT
    GATTTTAAAG CAGTCCGTTC TCGTGTCCCT GAAGATATTA GTAACTTTCT CCTGAGGTTG
    GCCATTTATG AACTCCAGAA AGAAGCTTCA TCCACAACAC GTCATGAAAT CAGCTGGATT
    GAGCCACTGG ACAAATCAAC TACTGCAACC AGACTAGCAG AAAAACCTGT GTGTGAAGAG
    CGACAGCAAG AGCCAGACGC CATAGACACC AGTGAAGATG ATATCTTTAT CAGTATTCCC
    ATGCCTCAGG AAGAACCCTG CTTGGGGAGT GATGTACCTC TGAAGTCACA TGAGCCAGTT
    CCTCAGAGCA GTGGAATGGA GCTGGCTTCC TGGAACCAGG ATAACCTGGA TAAGGACACA
    CAAGATCTCT TCAGCGATTC CCCTGTGAAG ACTGTGAGTC AGCCTGCAAA GAGAAAACTG
    CCTGACTGGA CTGCAGGACC TACAACATCA GTAACGACTA CATCGGAAAA GAGAACCAAA
    AAGAAGAAAG GACTTTTCAG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45771
    Clone ID Related Accession (Same CDS sequence) XM_009304279.2
    Accession Version XM_009304279.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product Werner syndrome ATP-dependent helicase isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304279.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTTCTAG AGGATGAGAA CATCATGAAA GTTGGCGTGG GAATTGAGGG TGACAAATGG 
    AAGTTATTGT CTGATTATGA CATCAAGTTA AAGAATATCG TTGATCTCTC TGATCTGGCA
    AATGAGAAGC TGAGATGTTG TGAGAAGTGG AGTTTGGATG GCCTTGTGAA GCACTTGTTG
    AAGAAGCAGC TCTTTAAGGA CAAACTGGTA CGCTGTAGTC ACTGGGATGA TTTTAGTCTG
    ACAGAAGACC AGAAGAGATA TGCTGCAACA GATGCTTACG CTGGATTGCT CATTTATCAG
    AAACTACAGG AAATGATATC TGGTTGCTCA GGATCCTCAG CGCTCAGTTT GAAAGAGAGA
    GTGTTTAAAA TTGCTTGTGA TCTAAAAGAT CTGGCTGATC ATATTCCCAA TGAACTTGAC
    TGCACAGACG GTGCTGGGAG ACTAGTGGAT GATCTGTCTC AAACTCTTAC ATCTCTAAGA
    TGTGTTTTGT CAAATGCCAA CAAGCCTACT GAACAATTAG TTGAAGAAAA GCCTTCTCCA
    GGACTTATTC TCGACGACAC CAGTTCTCAC CATGAGGGCG TCCCCACAAC TGAAGAGCAG
    AGCCACAGTC AGTGTGTGGA AAACCCTGGC AGACCTCCCC AGAGAGAGTG TGCGATGTCT
    CTGGATATCT CAGAGTACGA GCTGCAGATG CTGGAGGAAC AGGCCAGACA GGAGCAGCTG
    GAGGAGGAGT GTATCTTAGC TTTCCAGACT CAACACGCAT CGGGTCCCCA GGATTTAAAT
    GCGACAGAGA GCGATGAAGA GTTTGATGAT GAAATGATGA AGTGTGCAGA TGACATAGAA
    AAAATAAACA ATGCACAGAT GCCTCAGTCT GGTGCAGAGG ACGAAGATGA AGATGTTGAG
    GAGGACGAGG AAGAAGGGCT AGATTCAAGC ATTCCACAGC CAGTTCCTGA ACAAATCAAA
    TGTCTGAAAA TGTTCTTTGG ACATCACAGT TTCAAACCGG TTCAGTGGAA AGTGATTCAG
    TCCATTCTCA CAGAGAGGAG AGATAATCTT GTCGTCATGG CAACAGGTTA TGGGAAGAGT
    TTGTGCTTCC AGTTTCCTCC TGTGTACTGC CAGACAATCA GTGTGGTCAT TTCACCTCTC
    ATCGCCCTCA TGGAGGATCA AGTGCTTCAG CTCACGATGT CAAACATTCC TGCTTGTTTT
    CTTGGTTCTG CTCAGACCAA AAACCTAAAT GAGCAAGTGA AGAGGGGGGA TTTCAGAGTG
    GTGTACATGA CCCCTGAGTT CTGCTCGGGG AACATTCCTC TGCTAGTGCA GCTCGACAGG
    ACTGTTGGCT TATCACTGAT TGCGGTTGAT GAGGCTCATT GCATTTCTCA GTGGGGTCAT
    GATTTCAGAA GTGCGTATCG GGAGTTGGGC AAACTCAAGA AAAATCTCCC CACTGTCCCT
    TTTTTGGCAC TCACGGCTAC CGCAAGTCCA TCTATCCGTG ACGACATCAT TAAGAGTCTT
    CATCTGATCA ATCCCATGAT TACCTGTACT TCATTTGACC GACCAAACCT TTACTTGGAT
    GTCAATCGCA AGTCCGGGGA CGTCATCCAA GACCTCAAAC GATTTCTTGT TAAAAAGAAA
    GGGGGTGGTT ATGAGTTTGA GGGCTCTGCC ATTGTGTACT GTCCCTCAAA GAAAGAGGCA
    GAGCGTGTGA CAACAGCACT ATTCAAGCTG GACATCCCAT GTGGAGTTTA TCACGCTGGT
    CTGAGCATCA AACAGAGGAG AGAGACTCAG CACCAGTTCA TGAGGGATGA GATTCAGTGT
    GTGGTGGCCA CAGTGGCGTT TGGGATGGGC ATCAATAAAT CTGACATCAG GAAGGTCATC
    CATTATGGGG CTCCCAAAGA AATGGAGTCC TACTATCAGG AGATTGGCAG GGCTGGTAGA
    GACGGGCTGC CCAGTGCCTG TCACGTCTTA TGGATGCCCG GAGATATGGC GCTGAATAAA
    TTCATTCTCA ACCAATCCAA AAGTGAGCGC TTCAGGAGTT ACAAAATAGA CATGATGGCA
    AAAATGGAAA AGTATCTGAA TTCAACCAAA TGTAGAAGAA AGTTGATTTT GTGTCATTTT
    GAAGACAAGC GTCTCAGGAA AGTGACGTCT GGAATTCTGG GAAGCAGTCA ATGTTGCGAT
    AACTGCCGGT CAGGTGTGAA TGAAGAGGAT CTAGAGGTGC TCCAGCAGGA CTTTGGCTTG
    TGTGCATATC AGTTGATGAA GGCCATCAGT GTTATGGAGG AGAGGTTTGG CATCACAGCG
    CCGATCCTCT TCCTGCGTGG TTCATGTTCT CAGCGGGTTC CAGAACGCTT TCGGAAGCAC
    TCTCTCTTTG GTATTGGCAG GGACGTCTCA GAAACTTGGT GGAAAGCTCT TGGCCGAGAA
    CTTATTGCTG AGAAATATCT GATGGAAGTC ACAGGCCACA GCAAATTCAC CACTCTCTGT
    AAAATCACAA CTAAGGGAAG ATCATGGCTG AGTAAAGCTG AAGATGAGAA GCACAGACAG
    CTTTTGCTGC AGCCCAACAG AGATCTGTTC AGCAGAGTGT GTGCTCCGCG GAGAAAAGCA
    GATCCAGCAC AAGTTGAACC TACAGTTTCT AACCTGCAGT CACCTCACAT ACGGCCCTCT
    CTTGTTACAT TTCAGAGGAC AAAGCTTGGT TCTGAATCCC CTCAGATTGT GAAGAAAGGA
    GATTTGCCTT CTAGGTCAGC AGCCAGACAA ACACAGATTC AAACTGATGT CAGAGCCCAA
    ACTCCAGGAG CCTCTGCTAA AGAAATTCAG CTACAGTCTG AGCTGTATGG TAAGCTGGTG
    TCTGAGCGTC AGAAGTTAGC TAGTATCAGA GACATCCCAC CAGCTCTGCT GGCCACCAAT
    AAAATTCTTC TGGACATGTC AAAATTAAGG CCCTGCACGA TGTCCAGCTT GAAACAGGTG
    GATGGAGTTT CAGAAGCAAA AGCAGCCATG TTAACACCTC TCATAGATAT CATCGCCAAA
    TTCTGCCAGA CACACGGATT ACAGGTGGAT GTTTCCTCCA ACTCTGCTTC ATCTGTCCAA
    ACACACTCCA GCCGTGAATC AGTTGTAGGA TCTGTGGCTC AGTGTCTGCC GGACAGCATC
    TCCATCACAT ACAGCCTTTT CCAGATAGAG CACAAGAGCT TGAGGCAGGT ATCAGATGCT
    CGCTCTCTGC CCATTGCGGT GGTGGAGTCT CATCTTCTGC AAGCTCAGAA AATGGGCTGC
    GCTGTGGACA CTGACAGAGC AGGACTCTCA ACATCCATCA TGAACACCAT CAGAAAAAAC
    ACAGCCGGCT CAGATTTGTG TGATTTTAAA GCAGTCCGTT CTCGTGTCCC TGAAGATATT
    AGTAACTTTC TCCTGAGGTT GGCCATTTAT GAACTCCAGA AAGAAGCTTC ATCCACAACA
    CGTCATGAAA TCAGCTGGAT TGAGCCACTG GACAAATCAT CTACTGCAAC CAGACTAGCA
    GAAAAACCTG TGTGTGAAGA GCGACAGCAA GAGCCAGACG CCATAGACAC CAGTGAAGAT
    GATATCTTTA TCAGTATTCC CATGCCTCAG GAAGAACCCT GCTTGGGGAG TGATGTGCCT
    CTGAAGTCAC ATGAGCCAGT TCCTCAGAGC AGTGGAATGG AGCTGGCTTC CTGGAACCAG
    GATAACCTGG ATAAGGACAC ACAAGATCTC TTCAGCGATT CCCCTGTGAA GACTGTGAGT
    CAGCCTGCAA AGAGAAAACT GCCTGACTGG ACTGCAGGAC CTACAACATC AGTAACGACT
    ACATCGGAAA AGAGAACCAA AAAGAAGAAA GGACTTTTCA GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302554.1
    CDS59..3883
    Translation

    Target ORF information:

    RefSeq Version XM_009304279.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Werner syndrome (wrn), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304279.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTTCTAG AGGATGAGAA CATCATGAAA GTTGGCGTGG GAATTGAGGG TGACAAATGG 
    AAGTTATTGT CTGATTATGA CATCAAGTTA AAGAATATCG TTGATCTCTC TGATCTGGCA
    AATGAGAAGC TGAGATGTTG TGAGAAGTGG AGTTTGGATG GCCTTGTGAA GCACTTGTTG
    AAGAAGCAGC TCTTTAAGGA CAAACTGGTA CGCTGTAGTC ACTGGGATGA TTTTAGTCTG
    ACAGAAGACC AGAAGAGATA TGCTGCAACA GATGCTTACG CTGGATTGCT CATTTATCAG
    AAACTACAGG AAATGATATC TGGTTGCTCA GGATCCTCAG CGCTCAGTTT GAAAGAGAGA
    GTGTTTAAAA TTGCTTGTGA TCTAAAAGAT CTGGCTGATC ATATTCCCAA TGAACTTGAC
    TGCACAGACG GTGCTGGGAG ACTAGTGGAT GATCTGTCTC AAACTCTTAC ATCTCTAAGA
    TGTGTTTTGT CAAATGCCAA CAAGCCTACT GAACAATTAG TTGAAGAAAA GCCTTCTCCA
    GGACTTATTC TCGACGACAC CAGTTCTCAC CATGAGGGCG TCCCCACAAC TGAAGAGCAG
    AGCCACAGTC AGTGTGTGGA AAACCCTGGC AGACCTCCCC AGAGAGAGTG TGCGATGTCT
    CTGGATATCT CAGAGTACGA GCTGCAGATG CTGGAGGAAC AGGCCAGACA GGAGCAGCTG
    GAGGAGGAGT GTATCTTAGC TTTCCAGACT CAACACGCAT CGGGTCCCCA GGATTTAAAT
    GCGACAGAGA GCGATGAAGA GTTTGATGAT GAAATGATGA AGTGTGCAGA TGACATAGAA
    AAAATAAACA ATGCACAGAT GCCTCAGTCT GGTGCAGAGG ACGAAGATGA AGATGTTGAG
    GAGGACGAGG AAGAAGGGCT AGATTCAAGC ATTCCACAGC CAGTTCCTGA ACAAATCAAA
    TGTCTGAAAA TGTTCTTTGG ACATCACAGT TTCAAACCGG TTCAGTGGAA AGTGATTCAG
    TCCATTCTCA CAGAGAGGAG AGATAATCTT GTCGTCATGG CAACAGGTTA TGGGAAGAGT
    TTGTGCTTCC AGTTTCCTCC TGTGTACTGC CAGACAATCA GTGTGGTCAT TTCACCTCTC
    ATCGCCCTCA TGGAGGATCA AGTGCTTCAG CTCACGATGT CAAACATTCC TGCTTGTTTT
    CTTGGTTCTG CTCAGACCAA AAACCTAAAT GAGCAAGTGA AGAGGGGGGA TTTCAGAGTG
    GTGTACATGA CCCCTGAGTT CTGCTCGGGG AACATTCCTC TGCTAGTGCA GCTCGACAGG
    ACTGTTGGCT TATCACTGAT TGCGGTTGAT GAGGCTCATT GCATTTCTCA GTGGGGTCAT
    GATTTCAGAA GTGCGTATCG GGAGTTGGGC AAACTCAAGA AAAATCTCCC CACTGTCCCT
    TTTTTGGCAC TCACGGCTAC CGCAAGTCCA TCTATCCGTG ACGACATCAT TAAGAGTCTT
    CATCTGATCA ATCCCATGAT TACCTGTACT TCATTTGACC GACCAAACCT TTACTTGGAT
    GTCAATCGCA AGTCCGGGGA CGTCATCCAA GACCTCAAAC GATTTCTTGT TAAAAAGAAA
    GGGGGTGGTT ATGAGTTTGA GGGCTCTGCC ATTGTGTACT GTCCCTCAAA GAAAGAGGCA
    GAGCGTGTGA CAACAGCACT ATTCAAGCTG GACATCCCAT GTGGAGTTTA TCACGCTGGT
    CTGAGCATCA AACAGAGGAG AGAGACTCAG CACCAGTTCA TGAGGGATGA GATTCAGTGT
    GTGGTGGCCA CAGTGGCGTT TGGGATGGGC ATCAATAAAT CTGACATCAG GAAGGTCATC
    CATTATGGGG CTCCCAAAGA AATGGAGTCC TACTATCAGG AGATTGGCAG GGCTGGTAGA
    GACGGGCTGC CCAGTGCCTG TCACGTCTTA TGGATGCCCG GAGATATGGC GCTGAATAAA
    TTCATTCTCA ACCAATCCAA AAGTGAGCGC TTCAGGAGTT ACAAAATAGA CATGATGGCA
    AAAATGGAAA AGTATCTGAA TTCAACCAAA TGTAGAAGAA AGTTGATTTT GTGTCATTTT
    GAAGACAAGC GTCTCAGGAA AGTGACGTCT GGAATTCTGG GAAGCAGTCA ATGTTGCGAT
    AACTGCCGGT CAGGTGTGAA TGAAGAGGAT CTAGAGGTGC TCCAGCAGGA CTTTGGCTTG
    TGTGCATATC AGTTGATGAA GGCCATCAGT GTTATGGAGG AGAGGTTTGG CATCACAGCG
    CCGATCCTCT TCCTGCGTGG TTCATGTTCT CAGCGGGTTC CAGAACGCTT TCGGAAGCAC
    TCTCTCTTTG GTATTGGCAG GGACGTCTCA GAAACTTGGT GGAAAGCTCT TGGCCGAGAA
    CTTATTGCTG AGAAATATCT GATGGAAGTC ACAGGCCACA GCAAATTCAC CACTCTCTGT
    AAAATCACAA CTAAGGGAAG ATCATGGCTG AGTAAAGCTG AAGATGAGAA GCACAGACAG
    CTTTTGCTGC AGCCCAACAG AGATCTGTTC AGCAGAGTGT GTGCTCCGCG GAGAAAAGCA
    GATCCAGCAC AAGTTGAACC TACAGTTTCT AACCTGCAGT CACCTCACAT ACGGCCCTCT
    CTTGTTACAT TTCAGAGGAC AAAGCTTGGT TCTGAATCCC CTCAGATTGT GAAGAAAGGA
    GATTTGCCTT CTAGGTCAGC AGCCAGACAA ACACAGATTC AAACTGATGT CAGAGCCCAA
    ACTCCAGGAG CCTCTGCTAA AGAAATTCAG CTACAGTCTG AGCTGTATGG TAAGCTGGTG
    TCTGAGCGTC AGAAGTTAGC TAGTATCAGA GACATCCCAC CAGCTCTGCT GGCCACCAAT
    AAAATTCTTC TGGACATGTC AAAATTAAGG CCCTGCACGA TGTCCAGCTT GAAACAGGTG
    GATGGAGTTT CAGAAGCAAA AGCAGCCATG TTAACACCTC TCATAGATAT CATCGCCAAA
    TTCTGCCAGA CACACGGATT ACAGGTGGAT GTTTCCTCCA ACTCTGCTTC ATCTGTCCAA
    ACACACTCCA GCCGTGAATC AGTTGTAGGA TCTGTGGCTC AGTGTCTGCC GGACAGCATC
    TCCATCACAT ACAGCCTTTT CCAGATAGAG CACAAGAGCT TGAGGCAGGT ATCAGATGCT
    CGCTCTCTGC CCATTGCGGT GGTGGAGTCT CATCTTCTGC AAGCTCAGAA AATGGGCTGC
    GCTGTGGACA CTGACAGAGC AGGACTCTCA ACATCCATCA TGAACACCAT CAGAAAAAAC
    ACAGCCGGCT CAGATTTGTG TGATTTTAAA GCAGTCCGTT CTCGTGTCCC TGAAGATATT
    AGTAACTTTC TCCTGAGGTT GGCCATTTAT GAACTCCAGA AAGAAGCTTC ATCCACAACA
    CGTCATGAAA TCAGCTGGAT TGAGCCACTG GACAAATCAT CTACTGCAAC CAGACTAGCA
    GAAAAACCTG TGTGTGAAGA GCGACAGCAA GAGCCAGACG CCATAGACAC CAGTGAAGAT
    GATATCTTTA TCAGTATTCC CATGCCTCAG GAAGAACCCT GCTTGGGGAG TGATGTGCCT
    CTGAAGTCAC ATGAGCCAGT TCCTCAGAGC AGTGGAATGG AGCTGGCTTC CTGGAACCAG
    GATAACCTGG ATAAGGACAC ACAAGATCTC TTCAGCGATT CCCCTGTGAA GACTGTGAGT
    CAGCCTGCAA AGAGAAAACT GCCTGACTGG ACTGCAGGAC CTACAACATC AGTAACGACT
    ACATCGGAAA AGAGAACCAA AAAGAAGAAA GGACTTTTCA GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45770
    Clone ID Related Accession (Same CDS sequence) XM_009303971.2 , XM_021479248.1
    Accession Version XM_009303971.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product Werner syndrome ATP-dependent helicase isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGTGACA GGACTCTACC TAAATGGATG AATAACAAAC CTCAGGATGA ACACACATCA 
    GGAACTTATA AAAAGAATGT TTTGGAGGAT CATCTACCGT ATCTGGAATT TGGTGGAACA
    GTCGTATACA GCCAGGAAAG ACACGACTGT TCTTTTTTAT CAGAGGATTT AAGATCTGCT
    TTGTCGTCTG GCTCAGCGGT GGGATTTGAT CTGGAATGGC CACCAAGCTT CACCAAGGGG
    AAGACAAAGA AAGTGGCAAT GGTGCAGCTG TGTGCATCTG AGGACAAATG CTATTTGTTT
    CACATCTCCT CCATGTCAGG GTTTCCTCCT GGCCTGAAGA TGTTTCTAGA GGATGAGAAC
    ATCATGAAAG TTGGCGTGGG AATTGAGGGT GACAAATGGA AGTTATTGTC TGATTATGAC
    ATCAAGTTAA AGAATATCGT TGATCTCTCT GATCTGGCAA ATGAGAAGCT GAGATGTTGT
    GAGAAGTGGA GTTTGGATGG CCTTGTGAAG CACTTGTTGA AGAAGCAGCT CTTTAAGGAC
    AAACTGGTAC GCTGTAGTCA CTGGGATGAT TTTAGTCTGA CAGAAGACCA GAAGAGATAT
    GCTGCAACAG ATGCTTACGC TGGATTGCTC ATTTATCAGA AACTACAGGA AATGATATCT
    GGTTGCTCAG GATCCTCAGC GCTCAGTTTG AAAGAGAGAG TGTTTAAAAT TGCTTGTGAT
    CTAAAAGATC TGGCTGATCA TATTCCCAAT GAACTTGACT GCACAGACGG TGCTGGGAGA
    CTAGTGGATG ATCTGTCTCA AACTCTTACA TCTCTAAGAT GTGTTTTGTC AAATGCCAAC
    AAGCCTACTG AACAATTAGT TGAAGAAAAG CCTTCTCCAG GACTTATTCT CGACGACACC
    AGTTCTCACC ATGAGGGCGT CCCCACAACT GAAGAGCAGA GCCACAGTCA GTGTGTGGAA
    AACCCTGGCA GACCTCCCCA GAGAGAGTGT GCGATGTCTC TGGATATCTC AGAGTACGAG
    CTGCAGATGC TGGAGGAACA GGCCAGACAG GAGCAGCTGG AGGAGGAGTG TATCTTAGCT
    TTCCAGACTC AACACGCATC GGGTCCCCAG GATTTAAATG CGACAGAGAG CGATGAAGAG
    TTTGATGATG AAATGATGAA GTGTGCAGAT GACATAGAAA AAATAAACAA TGCACAGATG
    CCTCAGTCTG GTGCAGAGGA CGAAGATGAA GATGTTGAGG AGGACGAGGA AGAAGGGCTA
    GATTCAAGCA TTCCACAGCC AGTTCCTGAA CAAATCAAAT GTCTGAAAAT GTTCTTTGGA
    CATCACAGTT TCAAACCGGT TCAGTGGAAA GTGATTCAGT CCATTCTCAC AGAGAGGAGA
    GATAATCTTG TCGTCATGGC AACAGGTTAT GGGAAGAGTT TGTGCTTCCA GTTTCCTCCT
    GTGTACTGCC AGACAATCAG TGTGGTCATT TCACCTCTCA TCGCCCTCAT GGAGGATCAA
    GTGCTTCAGC TCACGATGTC AAACATTCCT GCTTGTTTTC TTGGTTCTGC TCAGACCAAA
    AACCTAAATG AGCAAGTGAA GAGGGGGGAT TTCAGAGTGG TGTACATGAC CCCTGAGTTC
    TGCTCGGGGA ACATTCCTCT GCTAGTGCAG CTCGACAGGA CTGTTGGCTT ATCACTGATT
    GCGGTTGATG AGGCTCATTG CATTTCTCAG TGGGGTCATG ATTTCAGAAG TGCGTATCGG
    GAGTTGGGCA AACTCAAGAA AAATCTCCCC ACTGTCCCTT TTTTGGCACT CACGGCTACC
    GCAAGTCCAT CTATCCGTGA CGACATCATT AAGAGTCTTC ATCTGATCAA TCCCATGATT
    ACCTGTACTT CATTTGACCG ACCAAACCTT TACTTGGATG TCAATCGCAA GTCCGGGGAC
    GTCATCCAAG ACCTCAAACG ATTTCTTGTT AAAAAGAAAG GGGGTGGTTA TGAGTTTGAG
    GGCTCTGCCA TTGTGTACTG TCCCTCAAAG AAAGAGGCAG AGCGTGTGAC AACAGCACTA
    TTCAAGCTGG ACATCCCATG TGGAGTTTAT CACGCTGGTC TGAGCATCAA ACAGAGGAGA
    GAGACTCAGC ACCAGTTCAT GAGGGATGAG ATTCAGTGTG TGGTGGCCAC AGTGGCGTTT
    GGGATGGGCA TCAATAAATC TGACATCAGG AAGGTCATCC ATTATGGGGC TCCCAAAGAA
    ATGGAGTCCT ACTATCAGGA GATTGGCAGG GCTGGTAGAG ACGGGCTGCC CAGTGCCTGT
    CACGTCTTAT GGATGCCCGG AGATATGGCG CTGAATAAAT TCATTCTCAA CCAATCCAAA
    AGTGAGCGCT TCAGGAGTTA CAAAATAGAC ATGATGGCAA AAATGGAAAA GTATCTGAAT
    TCAACCAAAT GTAGAAGAAA GTTGATTTTG TGTCATTTTG AAGACAAGCG TCTCAGGAAA
    GTGACGTCTG GAATTCTGGG AAGCAGTCAA TGTTGCGATA ACTGCCGGTC AGGTGTGAAT
    GAAGAGGATC TAGAGGTGCT CCAGCAGGAC TTTGGCTTGT GTGCATATCA GTTGATGAAG
    GCCATCAGTG TTATGGAGGA GAGGTTTGGC ATCACAGCGC CGATCCTCTT CCTGCGTGGT
    TCATGTTCTC AGCGGGTTCC AGAACGCTTT CGGAAGCACT CTCTCTTTGG TATTGGCAGG
    GACGTCTCAG AAACTTGGTG GAAAGCTCTT GGCCGAGAAC TTATTGCTGA GAAATATCTG
    ATGGAAGTCA CAGGCCACAG CAAATTCACC ACTCTCTGTA AAATCACAAC TAAGGGAAGA
    TCATGGCTGA GTAAAGCTGA AGATGAGAAG CACAGACAGC TTTTGCTGCA GCCCAACAGA
    GATCTGTTCA GCAGAGTGTG TGCTCCGCGG AGAAAAGCAG ATCCAGCACA AGTTGAACCT
    ACAGTTTCTA ACCTGCAGTC ACCTCACATA CGGCCCTCTC TTGTTACATT TCAGAGGACA
    AAGCTTGGTT CTGAATCCCC TCAGATTGTG AAGAAAGGAG ATTTGCCTTC TAGGTCAGCA
    GCCAGACAAA CACAGATTCA AACTGATGTC AGAGCCCAAA CTCCAGGAGC CTCTGCTAAA
    GAAATTCAGC TACAGTCTGA GCTGTATGGT AAGCTGGTGT CTGAGCGTCA GAAGTTAGCT
    AGTATCAGAG ACATCCCACC AGCTCTGCTG GCCACCAATA AAATTCTTCT GGACATGTCA
    AAATTAAGGC CCTGCACGAT GTCCAGCTTG AAACAGGTGG ATGGAGTTTC AGAAGCAAAA
    GCAGCCATGT TAACACCTCT CATAGATATC ATCGCCAAAT TCTGCCAGAC ACACGGATTA
    CAGGTGGATG TTTCCTCCAA CTCTGCTTCA TCTGTCCAAA CACACTCCAG CCGTGAATCA
    GTTGTAGGAT CTGTGGCTCA GTGTCTGCCG GACAGCATCT CCATCACATA CAGCCTTTTC
    CAGATAGAGC ACAAGAGCTT GAGGCAGGTA TCAGATGCTC GCTCTCTGCC CATTGCGGTG
    GTGGAGTCTC ATCTTCTGCA AGCTCAGAAA ATGGGCTGCG CTGTGGACAC TGACAGAGCA
    GGACTCTCAA CATCCATCAT GAACACCATC AGAAAAAACA CAGCCGGCTC AGATTTGTGT
    GATTTTAAAG CAGTCCGTTC TCGTGTCCCT GAAGATATTA GTAACTTTCT CCTGAGGTTG
    GCCATTTATG AACTCCAGAA AGAAGCTTCA TCCACAACAC GTCATGAAAT CAGCTGGATT
    GAGCCACTGG ACAAATCATC TACTGCAACC AGACTAGCAG AAAAACCTGT GTGTGAAGAG
    CGACAGCAAG AGCCAGACGC CATAGACACC AGTGAAGATG ATATCTTTAT CAGTATTCCC
    ATGCCTCAGG AAGAACCCTG CTTGGGGAGT GATGTGCCTC TGAAGTCACA TGAGCCAGTT
    CCTCAGAGCA GTGGAATGGA GCTGGCTTCC TGGAACCAGG ATAACCTGGA TAAGGACACA
    CAAGATCTCT TCAGCGATTC CCCTGTGAAG ACTGTGAGTC AGCCTGCAAA GAGAAAACTG
    CCTGACTGGA CTGCAGGACC TACAACATCA GTAACGACTA CATCGGAAAA GAGAACCAAA
    AAGAAGAAAG GACTTTTCAG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302246.1
    CDS332..4495
    Translation

    Target ORF information:

    RefSeq Version XM_009303971.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Werner syndrome (wrn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303971.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGTGACA GGACTCTACC TAAATGGATG AATAACAAAC CTCAGGATGA ACACACATCA 
    GGAACTTATA AAAAGAATGT TTTGGAGGAT CATCTACCGT ATCTGGAATT TGGTGGAACA
    GTCGTATACA GCCAGGAAAG ACACGACTGT TCTTTTTTAT CAGAGGATTT AAGATCTGCT
    TTGTCGTCTG GCTCAGCGGT GGGATTTGAT CTGGAATGGC CACCAAGCTT CACCAAGGGG
    AAGACAAAGA AAGTGGCAAT GGTGCAGCTG TGTGCATCTG AGGACAAATG CTATTTGTTT
    CACATCTCCT CCATGTCAGG GTTTCCTCCT GGCCTGAAGA TGTTTCTAGA GGATGAGAAC
    ATCATGAAAG TTGGCGTGGG AATTGAGGGT GACAAATGGA AGTTATTGTC TGATTATGAC
    ATCAAGTTAA AGAATATCGT TGATCTCTCT GATCTGGCAA ATGAGAAGCT GAGATGTTGT
    GAGAAGTGGA GTTTGGATGG CCTTGTGAAG CACTTGTTGA AGAAGCAGCT CTTTAAGGAC
    AAACTGGTAC GCTGTAGTCA CTGGGATGAT TTTAGTCTGA CAGAAGACCA GAAGAGATAT
    GCTGCAACAG ATGCTTACGC TGGATTGCTC ATTTATCAGA AACTACAGGA AATGATATCT
    GGTTGCTCAG GATCCTCAGC GCTCAGTTTG AAAGAGAGAG TGTTTAAAAT TGCTTGTGAT
    CTAAAAGATC TGGCTGATCA TATTCCCAAT GAACTTGACT GCACAGACGG TGCTGGGAGA
    CTAGTGGATG ATCTGTCTCA AACTCTTACA TCTCTAAGAT GTGTTTTGTC AAATGCCAAC
    AAGCCTACTG AACAATTAGT TGAAGAAAAG CCTTCTCCAG GACTTATTCT CGACGACACC
    AGTTCTCACC ATGAGGGCGT CCCCACAACT GAAGAGCAGA GCCACAGTCA GTGTGTGGAA
    AACCCTGGCA GACCTCCCCA GAGAGAGTGT GCGATGTCTC TGGATATCTC AGAGTACGAG
    CTGCAGATGC TGGAGGAACA GGCCAGACAG GAGCAGCTGG AGGAGGAGTG TATCTTAGCT
    TTCCAGACTC AACACGCATC GGGTCCCCAG GATTTAAATG CGACAGAGAG CGATGAAGAG
    TTTGATGATG AAATGATGAA GTGTGCAGAT GACATAGAAA AAATAAACAA TGCACAGATG
    CCTCAGTCTG GTGCAGAGGA CGAAGATGAA GATGTTGAGG AGGACGAGGA AGAAGGGCTA
    GATTCAAGCA TTCCACAGCC AGTTCCTGAA CAAATCAAAT GTCTGAAAAT GTTCTTTGGA
    CATCACAGTT TCAAACCGGT TCAGTGGAAA GTGATTCAGT CCATTCTCAC AGAGAGGAGA
    GATAATCTTG TCGTCATGGC AACAGGTTAT GGGAAGAGTT TGTGCTTCCA GTTTCCTCCT
    GTGTACTGCC AGACAATCAG TGTGGTCATT TCACCTCTCA TCGCCCTCAT GGAGGATCAA
    GTGCTTCAGC TCACGATGTC AAACATTCCT GCTTGTTTTC TTGGTTCTGC TCAGACCAAA
    AACCTAAATG AGCAAGTGAA GAGGGGGGAT TTCAGAGTGG TGTACATGAC CCCTGAGTTC
    TGCTCGGGGA ACATTCCTCT GCTAGTGCAG CTCGACAGGA CTGTTGGCTT ATCACTGATT
    GCGGTTGATG AGGCTCATTG CATTTCTCAG TGGGGTCATG ATTTCAGAAG TGCGTATCGG
    GAGTTGGGCA AACTCAAGAA AAATCTCCCC ACTGTCCCTT TTTTGGCACT CACGGCTACC
    GCAAGTCCAT CTATCCGTGA CGACATCATT AAGAGTCTTC ATCTGATCAA TCCCATGATT
    ACCTGTACTT CATTTGACCG ACCAAACCTT TACTTGGATG TCAATCGCAA GTCCGGGGAC
    GTCATCCAAG ACCTCAAACG ATTTCTTGTT AAAAAGAAAG GGGGTGGTTA TGAGTTTGAG
    GGCTCTGCCA TTGTGTACTG TCCCTCAAAG AAAGAGGCAG AGCGTGTGAC AACAGCACTA
    TTCAAGCTGG ACATCCCATG TGGAGTTTAT CACGCTGGTC TGAGCATCAA ACAGAGGAGA
    GAGACTCAGC ACCAGTTCAT GAGGGATGAG ATTCAGTGTG TGGTGGCCAC AGTGGCGTTT
    GGGATGGGCA TCAATAAATC TGACATCAGG AAGGTCATCC ATTATGGGGC TCCCAAAGAA
    ATGGAGTCCT ACTATCAGGA GATTGGCAGG GCTGGTAGAG ACGGGCTGCC CAGTGCCTGT
    CACGTCTTAT GGATGCCCGG AGATATGGCG CTGAATAAAT TCATTCTCAA CCAATCCAAA
    AGTGAGCGCT TCAGGAGTTA CAAAATAGAC ATGATGGCAA AAATGGAAAA GTATCTGAAT
    TCAACCAAAT GTAGAAGAAA GTTGATTTTG TGTCATTTTG AAGACAAGCG TCTCAGGAAA
    GTGACGTCTG GAATTCTGGG AAGCAGTCAA TGTTGCGATA ACTGCCGGTC AGGTGTGAAT
    GAAGAGGATC TAGAGGTGCT CCAGCAGGAC TTTGGCTTGT GTGCATATCA GTTGATGAAG
    GCCATCAGTG TTATGGAGGA GAGGTTTGGC ATCACAGCGC CGATCCTCTT CCTGCGTGGT
    TCATGTTCTC AGCGGGTTCC AGAACGCTTT CGGAAGCACT CTCTCTTTGG TATTGGCAGG
    GACGTCTCAG AAACTTGGTG GAAAGCTCTT GGCCGAGAAC TTATTGCTGA GAAATATCTG
    ATGGAAGTCA CAGGCCACAG CAAATTCACC ACTCTCTGTA AAATCACAAC TAAGGGAAGA
    TCATGGCTGA GTAAAGCTGA AGATGAGAAG CACAGACAGC TTTTGCTGCA GCCCAACAGA
    GATCTGTTCA GCAGAGTGTG TGCTCCGCGG AGAAAAGCAG ATCCAGCACA AGTTGAACCT
    ACAGTTTCTA ACCTGCAGTC ACCTCACATA CGGCCCTCTC TTGTTACATT TCAGAGGACA
    AAGCTTGGTT CTGAATCCCC TCAGATTGTG AAGAAAGGAG ATTTGCCTTC TAGGTCAGCA
    GCCAGACAAA CACAGATTCA AACTGATGTC AGAGCCCAAA CTCCAGGAGC CTCTGCTAAA
    GAAATTCAGC TACAGTCTGA GCTGTATGGT AAGCTGGTGT CTGAGCGTCA GAAGTTAGCT
    AGTATCAGAG ACATCCCACC AGCTCTGCTG GCCACCAATA AAATTCTTCT GGACATGTCA
    AAATTAAGGC CCTGCACGAT GTCCAGCTTG AAACAGGTGG ATGGAGTTTC AGAAGCAAAA
    GCAGCCATGT TAACACCTCT CATAGATATC ATCGCCAAAT TCTGCCAGAC ACACGGATTA
    CAGGTGGATG TTTCCTCCAA CTCTGCTTCA TCTGTCCAAA CACACTCCAG CCGTGAATCA
    GTTGTAGGAT CTGTGGCTCA GTGTCTGCCG GACAGCATCT CCATCACATA CAGCCTTTTC
    CAGATAGAGC ACAAGAGCTT GAGGCAGGTA TCAGATGCTC GCTCTCTGCC CATTGCGGTG
    GTGGAGTCTC ATCTTCTGCA AGCTCAGAAA ATGGGCTGCG CTGTGGACAC TGACAGAGCA
    GGACTCTCAA CATCCATCAT GAACACCATC AGAAAAAACA CAGCCGGCTC AGATTTGTGT
    GATTTTAAAG CAGTCCGTTC TCGTGTCCCT GAAGATATTA GTAACTTTCT CCTGAGGTTG
    GCCATTTATG AACTCCAGAA AGAAGCTTCA TCCACAACAC GTCATGAAAT CAGCTGGATT
    GAGCCACTGG ACAAATCATC TACTGCAACC AGACTAGCAG AAAAACCTGT GTGTGAAGAG
    CGACAGCAAG AGCCAGACGC CATAGACACC AGTGAAGATG ATATCTTTAT CAGTATTCCC
    ATGCCTCAGG AAGAACCCTG CTTGGGGAGT GATGTGCCTC TGAAGTCACA TGAGCCAGTT
    CCTCAGAGCA GTGGAATGGA GCTGGCTTCC TGGAACCAGG ATAACCTGGA TAAGGACACA
    CAAGATCTCT TCAGCGATTC CCCTGTGAAG ACTGTGAGTC AGCCTGCAAA GAGAAAACTG
    CCTGACTGGA CTGCAGGACC TACAACATCA GTAACGACTA CATCGGAAAA GAGAACCAAA
    AAGAAGAAAG GACTTTTCAG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45770
    Clone ID Related Accession (Same CDS sequence) XM_009303971.2 , XM_021479248.1
    Accession Version XM_021479248.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Werner syndrome ATP-dependent helicase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGTGACA GGACTCTACC TAAATGGATG AATAACAAAC CTCAGGATGA ACACACATCA 
    GGAACTTATA AAAAGAATGT TTTGGAGGAT CATCTACCGT ATCTGGAATT TGGTGGAACA
    GTCGTATACA GCCAGGAAAG ACACGACTGT TCTTTTTTAT CAGAGGATTT AAGATCTGCT
    TTGTCGTCTG GCTCAGCGGT GGGATTTGAT CTGGAATGGC CACCAAGCTT CACCAAGGGG
    AAGACAAAGA AAGTGGCAAT GGTGCAGCTG TGTGCATCTG AGGACAAATG CTATTTGTTT
    CACATCTCCT CCATGTCAGG GTTTCCTCCT GGCCTGAAGA TGTTTCTAGA GGATGAGAAC
    ATCATGAAAG TTGGCGTGGG AATTGAGGGT GACAAATGGA AGTTATTGTC TGATTATGAC
    ATCAAGTTAA AGAATATCGT TGATCTCTCT GATCTGGCAA ATGAGAAGCT GAGATGTTGT
    GAGAAGTGGA GTTTGGATGG CCTTGTGAAG CACTTGTTGA AGAAGCAGCT CTTTAAGGAC
    AAACTGGTAC GCTGTAGTCA CTGGGATGAT TTTAGTCTGA CAGAAGACCA GAAGAGATAT
    GCTGCAACAG ATGCTTACGC TGGATTGCTC ATTTATCAGA AACTACAGGA AATGATATCT
    GGTTGCTCAG GATCCTCAGC GCTCAGTTTG AAAGAGAGAG TGTTTAAAAT TGCTTGTGAT
    CTAAAAGATC TGGCTGATCA TATTCCCAAT GAACTTGACT GCACAGACGG TGCTGGGAGA
    CTAGTGGATG ATCTGTCTCA AACTCTTACA TCTCTAAGAT GTGTTTTGTC AAATGCCAAC
    AAGCCTACTG AACAATTAGT TGAAGAAAAG CCTTCTCCAG GACTTATTCT CGACGACACC
    AGTTCTCACC ATGAGGGCGT CCCCACAACT GAAGAGCAGA GCCACAGTCA GTGTGTGGAA
    AACCCTGGCA GACCTCCCCA GAGAGAGTGT GCGATGTCTC TGGATATCTC AGAGTACGAG
    CTGCAGATGC TGGAGGAACA GGCCAGACAG GAGCAGCTGG AGGAGGAGTG TATCTTAGCT
    TTCCAGACTC AACACGCATC GGGTCCCCAG GATTTAAATG CGACAGAGAG CGATGAAGAG
    TTTGATGATG AAATGATGAA GTGTGCAGAT GACATAGAAA AAATAAACAA TGCACAGATG
    CCTCAGTCTG GTGCAGAGGA CGAAGATGAA GATGTTGAGG AGGACGAGGA AGAAGGGCTA
    GATTCAAGCA TTCCACAGCC AGTTCCTGAA CAAATCAAAT GTCTGAAAAT GTTCTTTGGA
    CATCACAGTT TCAAACCGGT TCAGTGGAAA GTGATTCAGT CCATTCTCAC AGAGAGGAGA
    GATAATCTTG TCGTCATGGC AACAGGTTAT GGGAAGAGTT TGTGCTTCCA GTTTCCTCCT
    GTGTACTGCC AGACAATCAG TGTGGTCATT TCACCTCTCA TCGCCCTCAT GGAGGATCAA
    GTGCTTCAGC TCACGATGTC AAACATTCCT GCTTGTTTTC TTGGTTCTGC TCAGACCAAA
    AACCTAAATG AGCAAGTGAA GAGGGGGGAT TTCAGAGTGG TGTACATGAC CCCTGAGTTC
    TGCTCGGGGA ACATTCCTCT GCTAGTGCAG CTCGACAGGA CTGTTGGCTT ATCACTGATT
    GCGGTTGATG AGGCTCATTG CATTTCTCAG TGGGGTCATG ATTTCAGAAG TGCGTATCGG
    GAGTTGGGCA AACTCAAGAA AAATCTCCCC ACTGTCCCTT TTTTGGCACT CACGGCTACC
    GCAAGTCCAT CTATCCGTGA CGACATCATT AAGAGTCTTC ATCTGATCAA TCCCATGATT
    ACCTGTACTT CATTTGACCG ACCAAACCTT TACTTGGATG TCAATCGCAA GTCCGGGGAC
    GTCATCCAAG ACCTCAAACG ATTTCTTGTT AAAAAGAAAG GGGGTGGTTA TGAGTTTGAG
    GGCTCTGCCA TTGTGTACTG TCCCTCAAAG AAAGAGGCAG AGCGTGTGAC AACAGCACTA
    TTCAAGCTGG ACATCCCATG TGGAGTTTAT CACGCTGGTC TGAGCATCAA ACAGAGGAGA
    GAGACTCAGC ACCAGTTCAT GAGGGATGAG ATTCAGTGTG TGGTGGCCAC AGTGGCGTTT
    GGGATGGGCA TCAATAAATC TGACATCAGG AAGGTCATCC ATTATGGGGC TCCCAAAGAA
    ATGGAGTCCT ACTATCAGGA GATTGGCAGG GCTGGTAGAG ACGGGCTGCC CAGTGCCTGT
    CACGTCTTAT GGATGCCCGG AGATATGGCG CTGAATAAAT TCATTCTCAA CCAATCCAAA
    AGTGAGCGCT TCAGGAGTTA CAAAATAGAC ATGATGGCAA AAATGGAAAA GTATCTGAAT
    TCAACCAAAT GTAGAAGAAA GTTGATTTTG TGTCATTTTG AAGACAAGCG TCTCAGGAAA
    GTGACGTCTG GAATTCTGGG AAGCAGTCAA TGTTGCGATA ACTGCCGGTC AGGTGTGAAT
    GAAGAGGATC TAGAGGTGCT CCAGCAGGAC TTTGGCTTGT GTGCATATCA GTTGATGAAG
    GCCATCAGTG TTATGGAGGA GAGGTTTGGC ATCACAGCGC CGATCCTCTT CCTGCGTGGT
    TCATGTTCTC AGCGGGTTCC AGAACGCTTT CGGAAGCACT CTCTCTTTGG TATTGGCAGG
    GACGTCTCAG AAACTTGGTG GAAAGCTCTT GGCCGAGAAC TTATTGCTGA GAAATATCTG
    ATGGAAGTCA CAGGCCACAG CAAATTCACC ACTCTCTGTA AAATCACAAC TAAGGGAAGA
    TCATGGCTGA GTAAAGCTGA AGATGAGAAG CACAGACAGC TTTTGCTGCA GCCCAACAGA
    GATCTGTTCA GCAGAGTGTG TGCTCCGCGG AGAAAAGCAG ATCCAGCACA AGTTGAACCT
    ACAGTTTCTA ACCTGCAGTC ACCTCACATA CGGCCCTCTC TTGTTACATT TCAGAGGACA
    AAGCTTGGTT CTGAATCCCC TCAGATTGTG AAGAAAGGAG ATTTGCCTTC TAGGTCAGCA
    GCCAGACAAA CACAGATTCA AACTGATGTC AGAGCCCAAA CTCCAGGAGC CTCTGCTAAA
    GAAATTCAGC TACAGTCTGA GCTGTATGGT AAGCTGGTGT CTGAGCGTCA GAAGTTAGCT
    AGTATCAGAG ACATCCCACC AGCTCTGCTG GCCACCAATA AAATTCTTCT GGACATGTCA
    AAATTAAGGC CCTGCACGAT GTCCAGCTTG AAACAGGTGG ATGGAGTTTC AGAAGCAAAA
    GCAGCCATGT TAACACCTCT CATAGATATC ATCGCCAAAT TCTGCCAGAC ACACGGATTA
    CAGGTGGATG TTTCCTCCAA CTCTGCTTCA TCTGTCCAAA CACACTCCAG CCGTGAATCA
    GTTGTAGGAT CTGTGGCTCA GTGTCTGCCG GACAGCATCT CCATCACATA CAGCCTTTTC
    CAGATAGAGC ACAAGAGCTT GAGGCAGGTA TCAGATGCTC GCTCTCTGCC CATTGCGGTG
    GTGGAGTCTC ATCTTCTGCA AGCTCAGAAA ATGGGCTGCG CTGTGGACAC TGACAGAGCA
    GGACTCTCAA CATCCATCAT GAACACCATC AGAAAAAACA CAGCCGGCTC AGATTTGTGT
    GATTTTAAAG CAGTCCGTTC TCGTGTCCCT GAAGATATTA GTAACTTTCT CCTGAGGTTG
    GCCATTTATG AACTCCAGAA AGAAGCTTCA TCCACAACAC GTCATGAAAT CAGCTGGATT
    GAGCCACTGG ACAAATCATC TACTGCAACC AGACTAGCAG AAAAACCTGT GTGTGAAGAG
    CGACAGCAAG AGCCAGACGC CATAGACACC AGTGAAGATG ATATCTTTAT CAGTATTCCC
    ATGCCTCAGG AAGAACCCTG CTTGGGGAGT GATGTGCCTC TGAAGTCACA TGAGCCAGTT
    CCTCAGAGCA GTGGAATGGA GCTGGCTTCC TGGAACCAGG ATAACCTGGA TAAGGACACA
    CAAGATCTCT TCAGCGATTC CCCTGTGAAG ACTGTGAGTC AGCCTGCAAA GAGAAAACTG
    CCTGACTGGA CTGCAGGACC TACAACATCA GTAACGACTA CATCGGAAAA GAGAACCAAA
    AAGAAGAAAG GACTTTTCAG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334923.1
    CDS249..4412
    Translation

    Target ORF information:

    RefSeq Version XM_021479248.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Werner syndrome (wrn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479248.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGTGACA GGACTCTACC TAAATGGATG AATAACAAAC CTCAGGATGA ACACACATCA 
    GGAACTTATA AAAAGAATGT TTTGGAGGAT CATCTACCGT ATCTGGAATT TGGTGGAACA
    GTCGTATACA GCCAGGAAAG ACACGACTGT TCTTTTTTAT CAGAGGATTT AAGATCTGCT
    TTGTCGTCTG GCTCAGCGGT GGGATTTGAT CTGGAATGGC CACCAAGCTT CACCAAGGGG
    AAGACAAAGA AAGTGGCAAT GGTGCAGCTG TGTGCATCTG AGGACAAATG CTATTTGTTT
    CACATCTCCT CCATGTCAGG GTTTCCTCCT GGCCTGAAGA TGTTTCTAGA GGATGAGAAC
    ATCATGAAAG TTGGCGTGGG AATTGAGGGT GACAAATGGA AGTTATTGTC TGATTATGAC
    ATCAAGTTAA AGAATATCGT TGATCTCTCT GATCTGGCAA ATGAGAAGCT GAGATGTTGT
    GAGAAGTGGA GTTTGGATGG CCTTGTGAAG CACTTGTTGA AGAAGCAGCT CTTTAAGGAC
    AAACTGGTAC GCTGTAGTCA CTGGGATGAT TTTAGTCTGA CAGAAGACCA GAAGAGATAT
    GCTGCAACAG ATGCTTACGC TGGATTGCTC ATTTATCAGA AACTACAGGA AATGATATCT
    GGTTGCTCAG GATCCTCAGC GCTCAGTTTG AAAGAGAGAG TGTTTAAAAT TGCTTGTGAT
    CTAAAAGATC TGGCTGATCA TATTCCCAAT GAACTTGACT GCACAGACGG TGCTGGGAGA
    CTAGTGGATG ATCTGTCTCA AACTCTTACA TCTCTAAGAT GTGTTTTGTC AAATGCCAAC
    AAGCCTACTG AACAATTAGT TGAAGAAAAG CCTTCTCCAG GACTTATTCT CGACGACACC
    AGTTCTCACC ATGAGGGCGT CCCCACAACT GAAGAGCAGA GCCACAGTCA GTGTGTGGAA
    AACCCTGGCA GACCTCCCCA GAGAGAGTGT GCGATGTCTC TGGATATCTC AGAGTACGAG
    CTGCAGATGC TGGAGGAACA GGCCAGACAG GAGCAGCTGG AGGAGGAGTG TATCTTAGCT
    TTCCAGACTC AACACGCATC GGGTCCCCAG GATTTAAATG CGACAGAGAG CGATGAAGAG
    TTTGATGATG AAATGATGAA GTGTGCAGAT GACATAGAAA AAATAAACAA TGCACAGATG
    CCTCAGTCTG GTGCAGAGGA CGAAGATGAA GATGTTGAGG AGGACGAGGA AGAAGGGCTA
    GATTCAAGCA TTCCACAGCC AGTTCCTGAA CAAATCAAAT GTCTGAAAAT GTTCTTTGGA
    CATCACAGTT TCAAACCGGT TCAGTGGAAA GTGATTCAGT CCATTCTCAC AGAGAGGAGA
    GATAATCTTG TCGTCATGGC AACAGGTTAT GGGAAGAGTT TGTGCTTCCA GTTTCCTCCT
    GTGTACTGCC AGACAATCAG TGTGGTCATT TCACCTCTCA TCGCCCTCAT GGAGGATCAA
    GTGCTTCAGC TCACGATGTC AAACATTCCT GCTTGTTTTC TTGGTTCTGC TCAGACCAAA
    AACCTAAATG AGCAAGTGAA GAGGGGGGAT TTCAGAGTGG TGTACATGAC CCCTGAGTTC
    TGCTCGGGGA ACATTCCTCT GCTAGTGCAG CTCGACAGGA CTGTTGGCTT ATCACTGATT
    GCGGTTGATG AGGCTCATTG CATTTCTCAG TGGGGTCATG ATTTCAGAAG TGCGTATCGG
    GAGTTGGGCA AACTCAAGAA AAATCTCCCC ACTGTCCCTT TTTTGGCACT CACGGCTACC
    GCAAGTCCAT CTATCCGTGA CGACATCATT AAGAGTCTTC ATCTGATCAA TCCCATGATT
    ACCTGTACTT CATTTGACCG ACCAAACCTT TACTTGGATG TCAATCGCAA GTCCGGGGAC
    GTCATCCAAG ACCTCAAACG ATTTCTTGTT AAAAAGAAAG GGGGTGGTTA TGAGTTTGAG
    GGCTCTGCCA TTGTGTACTG TCCCTCAAAG AAAGAGGCAG AGCGTGTGAC AACAGCACTA
    TTCAAGCTGG ACATCCCATG TGGAGTTTAT CACGCTGGTC TGAGCATCAA ACAGAGGAGA
    GAGACTCAGC ACCAGTTCAT GAGGGATGAG ATTCAGTGTG TGGTGGCCAC AGTGGCGTTT
    GGGATGGGCA TCAATAAATC TGACATCAGG AAGGTCATCC ATTATGGGGC TCCCAAAGAA
    ATGGAGTCCT ACTATCAGGA GATTGGCAGG GCTGGTAGAG ACGGGCTGCC CAGTGCCTGT
    CACGTCTTAT GGATGCCCGG AGATATGGCG CTGAATAAAT TCATTCTCAA CCAATCCAAA
    AGTGAGCGCT TCAGGAGTTA CAAAATAGAC ATGATGGCAA AAATGGAAAA GTATCTGAAT
    TCAACCAAAT GTAGAAGAAA GTTGATTTTG TGTCATTTTG AAGACAAGCG TCTCAGGAAA
    GTGACGTCTG GAATTCTGGG AAGCAGTCAA TGTTGCGATA ACTGCCGGTC AGGTGTGAAT
    GAAGAGGATC TAGAGGTGCT CCAGCAGGAC TTTGGCTTGT GTGCATATCA GTTGATGAAG
    GCCATCAGTG TTATGGAGGA GAGGTTTGGC ATCACAGCGC CGATCCTCTT CCTGCGTGGT
    TCATGTTCTC AGCGGGTTCC AGAACGCTTT CGGAAGCACT CTCTCTTTGG TATTGGCAGG
    GACGTCTCAG AAACTTGGTG GAAAGCTCTT GGCCGAGAAC TTATTGCTGA GAAATATCTG
    ATGGAAGTCA CAGGCCACAG CAAATTCACC ACTCTCTGTA AAATCACAAC TAAGGGAAGA
    TCATGGCTGA GTAAAGCTGA AGATGAGAAG CACAGACAGC TTTTGCTGCA GCCCAACAGA
    GATCTGTTCA GCAGAGTGTG TGCTCCGCGG AGAAAAGCAG ATCCAGCACA AGTTGAACCT
    ACAGTTTCTA ACCTGCAGTC ACCTCACATA CGGCCCTCTC TTGTTACATT TCAGAGGACA
    AAGCTTGGTT CTGAATCCCC TCAGATTGTG AAGAAAGGAG ATTTGCCTTC TAGGTCAGCA
    GCCAGACAAA CACAGATTCA AACTGATGTC AGAGCCCAAA CTCCAGGAGC CTCTGCTAAA
    GAAATTCAGC TACAGTCTGA GCTGTATGGT AAGCTGGTGT CTGAGCGTCA GAAGTTAGCT
    AGTATCAGAG ACATCCCACC AGCTCTGCTG GCCACCAATA AAATTCTTCT GGACATGTCA
    AAATTAAGGC CCTGCACGAT GTCCAGCTTG AAACAGGTGG ATGGAGTTTC AGAAGCAAAA
    GCAGCCATGT TAACACCTCT CATAGATATC ATCGCCAAAT TCTGCCAGAC ACACGGATTA
    CAGGTGGATG TTTCCTCCAA CTCTGCTTCA TCTGTCCAAA CACACTCCAG CCGTGAATCA
    GTTGTAGGAT CTGTGGCTCA GTGTCTGCCG GACAGCATCT CCATCACATA CAGCCTTTTC
    CAGATAGAGC ACAAGAGCTT GAGGCAGGTA TCAGATGCTC GCTCTCTGCC CATTGCGGTG
    GTGGAGTCTC ATCTTCTGCA AGCTCAGAAA ATGGGCTGCG CTGTGGACAC TGACAGAGCA
    GGACTCTCAA CATCCATCAT GAACACCATC AGAAAAAACA CAGCCGGCTC AGATTTGTGT
    GATTTTAAAG CAGTCCGTTC TCGTGTCCCT GAAGATATTA GTAACTTTCT CCTGAGGTTG
    GCCATTTATG AACTCCAGAA AGAAGCTTCA TCCACAACAC GTCATGAAAT CAGCTGGATT
    GAGCCACTGG ACAAATCATC TACTGCAACC AGACTAGCAG AAAAACCTGT GTGTGAAGAG
    CGACAGCAAG AGCCAGACGC CATAGACACC AGTGAAGATG ATATCTTTAT CAGTATTCCC
    ATGCCTCAGG AAGAACCCTG CTTGGGGAGT GATGTGCCTC TGAAGTCACA TGAGCCAGTT
    CCTCAGAGCA GTGGAATGGA GCTGGCTTCC TGGAACCAGG ATAACCTGGA TAAGGACACA
    CAAGATCTCT TCAGCGATTC CCCTGTGAAG ACTGTGAGTC AGCCTGCAAA GAGAAAACTG
    CCTGACTGGA CTGCAGGACC TACAACATCA GTAACGACTA CATCGGAAAA GAGAACCAAA
    AAGAAGAAAG GACTTTTCAG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.