LOC568664 cDNA ORF clone, Danio rerio(zebrafish)

The following LOC568664 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC568664 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa146925 XM_021471020.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa146926 XM_021471021.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa146927 XM_021471023.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa146927 XM_021471022.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa146928 XM_021471024.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa146929 XM_021471025.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa146930 XM_021471026.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa146931 XM_021471028.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa146932 XM_021471029.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa146933 XM_021471030.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa146934 XM_021471031.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa146935 XM_021471032.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa146936 XM_021471033.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa146937 XM_021471034.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa146938 XM_021471035.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa146939 XM_021471036.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa146940 XM_021471037.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa146941 XM_021471038.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa146942 XM_021471039.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa45489 XM_009298420.2
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa45490 XM_009298421.2
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa45491 XM_009298422.2
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa45492 XM_009298423.2
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa45493 XM_009298424.2
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa45494 XM_009298425.2
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa45495 XM_009298426.2
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa45496 XM_009298427.2
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa45497 XM_009298428.2
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa45498 XM_009298429.2
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa45501 XM_009298432.2
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa45502 XM_009298433.2
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa45505 XM_009298436.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
ODa53641 XM_017354578.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa53642 XM_017354579.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa53643 XM_017354580.1
Latest version!
Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa146925
    Clone ID Related Accession (Same CDS sequence) XM_021471020.1
    Accession Version XM_021471020.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG
    ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA
    CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT
    GAAGCCTGTA CACATCTGAA TAAAGTTCTG GGTGGAAACC TGTTACTCTT GAGAGAGCTG
    AATCTGAGTA AACTTGATCT TGGACCATCA GGATTGGAGA AACTTGCTGC TGTACTGAAA
    GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCAGTATGTC AGAAGAAGAT
    TGTCGTGTTT TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGACAGA GCTGAATTTG
    AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CCACTCTGTT TCAAAACCAA
    CAGTGTAGAC TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT
    GCTTTGGCTT CAGCATTTAA CTCAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGAG
    AATAAACTAG GAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCGCAGTGC
    ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGGATCCACA CTGTACACTG
    AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGACGCCT GTCGGTTTGT GACTAAAATT
    GTGGGTAAAA ACCCATTACT CCTGACAGAA CTGAATCTGA GTGATCGTAA ACTAGGAGAC
    ACACGAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGTAGAGT CAACACAATA
    ATGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTAACTTC AGCATTTATT
    TTAAACCCAT CACACCTGAT AGAGCTCAAT CTGAGTGGGA ATAAACTAGG AGACTCAGGA
    GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT ACACAATGCC GATTAGAGAA ACTGAGTCTT
    TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC
    CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG
    AAGCTCACTG ATTTACTGCA CAGTAGAAGC TGTAAATTCA GATTTCTGAA GAGTTCTGCT
    GCACAGGAAG CATGTGACTA TCTTACTAAA GTTCTGGGTA TAAGTCCATT ATTACTGACA
    GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT
    CTGATGGACT CTCATAGCAA AGTGAAGAAA ATCAAGCTGA ATAATTGTGA GCTGACAGAG
    AAAAGCTGTT CACTTCTAGC TGCAGTTCTC TCCTCCAAAA CCATCCTGAA AGAGATGAAC
    CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCAGAGAAA TCTGTGAGGG ACTGAAGAAT
    CCTGTGTGTG AGCTGAAAAT ACTCAGTCTT TCAGACTGCC GTATCAGTGA AGAAGGTTAT
    AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA
    GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ACTTACTGGA GGATCCACAC
    TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCACTTTGTG
    ACTGAAATGG TGGGTAAAAA CCCATTACTC CTGAAAGAAC TGAATCTGAG TGATCGTGTA
    CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTGCTGC AGGATAAACA CTGTCAGATC
    CACACACTCA TTCTGTGTTA TTGCAGTATT ACAGAGGAAC AGTGTGTGAT CCTGACTTCA
    GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAGTGGAAA CAATCTAGGA
    GACTCTGCAG TGAAAAAACT CAGTGATTTA CTGATGAACC AACAATTCAA GCTGGAGAAA
    CTACGTCTGA GTGATTGCAG TATTACAGAG AAACAGTGTG TGATCCTGAC TTCAGCTCTG
    AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAATG GGAATAGACT AGGAGACTCT
    GGAGCTTATG GACTTGGCCA TCTGCTGAGC AGTGTGGATT GCAAGCTGAA TAAACTACAT
    CTGTGTGATT GCAGTATTAC AGGGAAACAG TTGCACTTCC TGATTGCAGT TATGTGTATG
    GACCCATCAC ACCTGAGGGT ACTGGACCTC AGTAAGAATC AAATAAAAAG CAGAGGAATG
    AACATGCTGT GTGATGTGCT GAATAAACCA CGCTGTAAAC TCAAGAGTTT AAGACTAAAT
    GACTGTGGCA TTACAGATGT TACACACTTA ACTCAGTCTT TGGCTGAGAA CAAAGCACTG
    GAGTTCTTAA AAGAGCTTGA ACTGAGTAAA AATCAAATGG GAGACTCCGA AAAGCAAAAA
    CTCAGTGACC TGCTGCGAGA CTCAAACTGT AAACTGAGGT TAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326695.1
    CDS182..7489
    Translation

    Target ORF information:

    RefSeq Version XM_021471020.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471020.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG
    ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA
    CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT
    GAAGCCTGTA CACATCTGAA TAAAGTTCTG GGTGGAAACC TGTTACTCTT GAGAGAGCTG
    AATCTGAGTA AACTTGATCT TGGACCATCA GGATTGGAGA AACTTGCTGC TGTACTGAAA
    GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCAGTATGTC AGAAGAAGAT
    TGTCGTGTTT TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGACAGA GCTGAATTTG
    AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CCACTCTGTT TCAAAACCAA
    CAGTGTAGAC TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT
    GCTTTGGCTT CAGCATTTAA CTCAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGAG
    AATAAACTAG GAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCGCAGTGC
    ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGGATCCACA CTGTACACTG
    AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGACGCCT GTCGGTTTGT GACTAAAATT
    GTGGGTAAAA ACCCATTACT CCTGACAGAA CTGAATCTGA GTGATCGTAA ACTAGGAGAC
    ACACGAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGTAGAGT CAACACAATA
    ATGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTAACTTC AGCATTTATT
    TTAAACCCAT CACACCTGAT AGAGCTCAAT CTGAGTGGGA ATAAACTAGG AGACTCAGGA
    GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT ACACAATGCC GATTAGAGAA ACTGAGTCTT
    TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC
    CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG
    AAGCTCACTG ATTTACTGCA CAGTAGAAGC TGTAAATTCA GATTTCTGAA GAGTTCTGCT
    GCACAGGAAG CATGTGACTA TCTTACTAAA GTTCTGGGTA TAAGTCCATT ATTACTGACA
    GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT
    CTGATGGACT CTCATAGCAA AGTGAAGAAA ATCAAGCTGA ATAATTGTGA GCTGACAGAG
    AAAAGCTGTT CACTTCTAGC TGCAGTTCTC TCCTCCAAAA CCATCCTGAA AGAGATGAAC
    CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCAGAGAAA TCTGTGAGGG ACTGAAGAAT
    CCTGTGTGTG AGCTGAAAAT ACTCAGTCTT TCAGACTGCC GTATCAGTGA AGAAGGTTAT
    AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA
    GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ACTTACTGGA GGATCCACAC
    TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCACTTTGTG
    ACTGAAATGG TGGGTAAAAA CCCATTACTC CTGAAAGAAC TGAATCTGAG TGATCGTGTA
    CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTGCTGC AGGATAAACA CTGTCAGATC
    CACACACTCA TTCTGTGTTA TTGCAGTATT ACAGAGGAAC AGTGTGTGAT CCTGACTTCA
    GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAGTGGAAA CAATCTAGGA
    GACTCTGCAG TGAAAAAACT CAGTGATTTA CTGATGAACC AACAATTCAA GCTGGAGAAA
    CTACGTCTGA GTGATTGCAG TATTACAGAG AAACAGTGTG TGATCCTGAC TTCAGCTCTG
    AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAATG GGAATAGACT AGGAGACTCT
    GGAGCTTATG GACTTGGCCA TCTGCTGAGC AGTGTGGATT GCAAGCTGAA TAAACTACAT
    CTGTGTGATT GCAGTATTAC AGGGAAACAG TTGCACTTCC TGATTGCAGT TATGTGTATG
    GACCCATCAC ACCTGAGGGT ACTGGACCTC AGTAAGAATC AAATAAAAAG CAGAGGAATG
    AACATGCTGT GTGATGTGCT GAATAAACCA CGCTGTAAAC TCAAGAGTTT AAGACTAAAT
    GACTGTGGCA TTACAGATGT TACACACTTA ACTCAGTCTT TGGCTGAGAA CAAAGCACTG
    GAGTTCTTAA AAGAGCTTGA ACTGAGTAAA AATCAAATGG GAGACTCCGA AAAGCAAAAA
    CTCAGTGACC TGCTGCGAGA CTCAAACTGT AAACTGAGGT TAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146926
    Clone ID Related Accession (Same CDS sequence) XM_021471021.1
    Accession Version XM_021471021.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7239bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGAGCCAAA ACTTTCGATT AACTTACATT TTAGAAAACA TTGAAGGATC TCAGGTGTGT 
    TTGAGCTGTG ACTTAGTCTT TTTAAAGGTT TCTGGATTCT GTTTCTGCTG CACAAGACCG
    TCTTCTCGAC ATGTAAGATC AGGTTCGTAT GTATCCAGCT CTGTGTCTGT GAAGAGTGAT
    GATTCAAAAA AAGGAGGTCA GCCAAACTTC AGTAGAGAAA CACTGTCACC TACCACAAGT
    GTTCAAAATG AGTCTTCAGA CACAGACATC CAGACTCAGA AAAAACACAT GAGTTTTAAA
    GACAATCTTC AGGGGATCTT CCAGGATCTT GAGAACCAAA TATTAAAATT TCTGAAGAAT
    GAGCTAGAAA AGTTTAAGAA AATATTACAT GAAGAAAATA CAGAATACTT TGTAAAGGAC
    TTTGGTGAGA ACAGATGCAG AATAAAAGAA GCAGCTCTTG ATCTCACACT CTACTTCCTG
    AAAGAAATGA AGCAAGATGA AGCAGCAGAT ACTCTAGAAG ATGAGCTGTT CTTCATTCAT
    CAGCTGAAAT GTGGCCTACA GAAGAAGTAT CAGTGTGTGT TTGAAGGAAT TGCACAGCAA
    GGAGACTCCA CACTCCTGAA GAACATCTAC ACAGATCTCT ATATCACTCA GGGTGGCAGT
    GAACAGGTCA ATACTGAACA TGAGGTCAGG CAGATTGAAG TTACTTCCAG ACGTCATAAA
    GCTCAAGAGA TACAGGTTGA ATGCAGACAT TTGTTTGAAG CGTCTGAACA AGACAAGCAG
    ATCAGAACTG TACTGACAAA AGGAGTCGCT GGCATCGGGA AATCAGTCTC TGTGCAAAAG
    TTTGTTCTGG ATTGGGCTGA AGGAAAAGAA AATCAAGAGA TCAGCTTCAT ATTTCCTCTT
    CCATTCAGAG AGATGAACTT AAAGGAGAAA GAAACACCCA GTTTGATGGA GCTTATAACT
    CAGTTTTACC CAGAGACAAA AGGACTGAAC CTTACAAGAA GGAATCGATT CAAAGTCCTG
    TTCATCCTTG ATGGATTGGA TGAATGTCGT CTTCCTCTGA ACTTTGCTGG TAATGAGACG
    TGTCGTGATG TTTCGTCACC AGCCTCTCTG GATGTTCTCC TGACGAACCT CATCAAGGGA
    AATCTGCTTC CTTCTGCTCT CATCTGGATC ACCAGCAGAC CAGCAGCTGC CAGTAAGATT
    CCTCCTGACT GTATCGACAG AGTGACAGAG ATACGAGGAT TCAATGATGC ACAAAAGGAG
    GAGTACTTCA GAAAAAGACT CACAGATGAG AGTCTGGCCA AAGAAATCAT TGATCATGTT
    AAAAAATCAA AGAGTCTTTT CATCATGTGC CACATCCCAG TCTTCTGCTG GATTTCAGCC
    ACTGTTCTCC AGAACATTTT ACAGAAGAAG AGAAAAAATG TGAAAAACAA TCAGGCTGAT
    GACGCCTCCA AAACACTGCA GGAATCAAAC ACTGAAGACA CTCCCAAGAC TCTGACACAA
    ATGTACACAC ACTTCTTGCA CGTTCAGATC AAGCAGAGCA GACAAAAGTA TGATGGAGAA
    AACAAACCAG ATGTGTCCTG GGATAAAGAC GCCATCCTTT CACTGGGGAA ACTGGCATTT
    CAGCAGCTGC TAAACAACAA CCTGATCTTC TATGAAACAG ACCTGGAAGC CTGTGGTATT
    GACGTCTATA AAGCTGAAAA GTTCTCAGGC ATGTGTACTG AGATCTTTAA GGAGGAAACA
    GGGATTGTTC TTGGTACCAT GTACTGCTTT GTTCACTTGA GTGTTCAAGA GTTTATTGCA
    GCCCTTTATG CACATCTGAT TCTGAACATC AACAATAGAA GTGTGTTTGA TCAAAACACA
    AGAAATAGAA ATGTGTCCAT GATTGATTTG CTGAAGACTG CAGTGGACAA GGCACTTGAG
    AGTGACAATG GACACCTGGA CCTTTTCCTT CGCTTCCTCC TTGGTCTGTC ACTCCAGTCC
    AACAGTGGAA TCTTAAAAGG CCTGTTGACA CAGCAAGACC ACAATAACTG GAGCAAATGG
    AAAATACTTC AGTACTTCTT TAGAACAGTA GATCAGCAGC AAGACCACAA TGACCAGATC
    AAAGAGGAAA TAGTTCATTA CATCAAGCAG AAGTTTGAAG CAAATCTTTC TCTTGAGAGA
    TCCATCAACC TGTTCCACTG TCTGAATGAA CTAAACGATC AAACTCTGGT GAAAGAGATT
    CAGTCTCACC TTAGCAAAGG AAGGTACTCA TCTTCTGACC TTTCACCTGC CCAGTGGTCT
    GCTCTGGTCT TTGTGTTGTT GACATCAGAT GAAGAGATGG AGGAGTTTGA GCTTCAGAAA
    TTCAAGAAAT CAGACGAGTG TCTCATCAGG CTATTAGCTG TCATCAAAAC CTGCAAAAGA
    GCACTGCTCA GTAAGTGTAA TCTAACAGAG GAAAGCTGTT CAGCTCTGGC TTCAGTTCTC
    AGATCAGACT CCAGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGGATTCA
    GGAGTGAAGC TGCTCTCTGA TGGACTGAAG GACAGTAACT GTACACTGGA GAAACTCAGA
    CTTTCAGAGT GCGGTATCAA TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA
    AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACC ATCAGGAGTG
    AAGGAGCTCA CTGATTTACT GAAACCAGAC TATACACTTA AAACACTGAG GTTTTTGAGT
    CCTGCTGCAG AAGAAGTCTT TCAGTTTGTG ACTAAAATTG TGGGTAAAAA CCCGTTACTC
    CTGAAAGAAC TGAATCTGAG TGATCGTGAA CTAGGAGATA CACGAGTGAA TCAACTCTCT
    GCTCTGCTGC AGGATAAACA CTGTAGACTC AACACACTCA TGCTGAATAA TAACAGGATT
    ACAGCAGAAG GTTGTGCTGC TTTGACTTTT GCATTTAATT TAAATCCTTC ACACCTGATA
    GAGCTCAATC TGAGTGGGAA TAAACTAGGA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG
    CTGGAAAATA CACAATGCCA ATTAGAGAAA CTGAGGCTTT CAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA TTTACAGCAG
    GATCCACACT GTACACTGAA GATACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGCCTGT
    CAGTTTTTGA CTGGAGTTGT GGGTAAAAAC CTGTTACTCT TGAAAGAGCT GAATCTGAGT
    GGACATGAAC TAGGACTCTC AGACAGCTTT CAAATGCTTG TTCTTTTACT GCATGATAAA
    CACTGTACGC TCAACACACT GATTCTTTCA GATTGCAGTA TCACAGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAC ATCAAACCCA TCATACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGGAA CTCACTGATT TACTACTGGA TGAACGCTGT
    AGCTTAAAAA TCATCAGGTT TCTGAAAAGT CCTGCTGCAC AAGAGTCATG TGATTTTCTC
    ACTGAAGTTC TGGATGTAAA TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGATAAA
    TTAGGAGATC TGGATGGAGA GAAAATCTCT GCTTTTTTGA TGGACCCTCA TAGCAAAGTG
    GAGAAAATCA AGCTGAATAA TTGTAAGCTG CTTGAGAAAA GCTCATCAGT TTTAGCAACA
    GTTCTGTCTT CCAAAACCAT CCTGAAAGAG CTGAACCTGA ACAACAGTCG TCTGCTGGAC
    TCAGGAGTCA GAGAGATCTG TGAGGGACTG AAGAATCCTG TGTGTGAGCT GAAGATACTC
    AAGCTTTCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT
    TTGAGTCCTG CTGCTGATGA AGGCTGTCAG TTTGTGACTG GAATTGTGGG TAAAAACCCA
    TTACTTCTGA AAGAGCTAAA TCTGAGTGAT CGTGAACTAG GAGACACACG AGTGAATCAA
    CTCTCTGCTC TACTGCAGGA TAAACACTGT AAACTCAACA CACTCATTCT TAGTAAATGT
    GGTGTAACAG TGGAAAGCTG TTCAGCTCTG GCTACAGTCC TGAGATCAGA GACCAGTCTA
    AAAGAGCTTG ACATGAGCAA CAATAATCTG CAGGAATCAG GAGTGAAGAA ACTCCAGAGC
    GGATTAAAAA ACACAAACTG CACACTGGAA AAACTCAGTC TTTTAGACTG CAGTATCAGT
    GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG
    CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGAAGCTCAC TGAGTTACTG
    AAACCAGACT ATACACTGAA GACACTGAGG TTTTTGAGTG ATGATGCAGA TGAAGCCTGT
    ACACATCTGA ATAAAGTTCT GGGTGGAAAC CTGTTACTCT TGAGAGAGCT GAATCTGAGT
    AAACTTGATC TTGGACCATC AGGATTGGAG AAACTTGCTG CTGTACTGAA AGATAAACAC
    TGTAAACTCA ACACACTGAT TCTGAATAAC AGCAGTATGT CAGAAGAAGA TTGTCGTGTT
    TTGGCTGAAG CTTTTAATTC AAACCCATCA AATCTGACAG AGCTGAATTT GAGTGGGACT
    AAACTCACAA ACTCAGGAAT GAAGAGCTTC ACCACTCTGT TTCAAAACCA ACAGTGTAGA
    CTGGAAAAGC TGAAGTTCAG CAGCATGTGT ATTACAGCAG AAGGTTGTGC TGCTTTGGCT
    TCAGCATTTA ACTCAAATCC TTCACACCTG ATAGAGCTCA ATCTGAGTGA GAATAAACTA
    GGAGACTCAG GAGTAACAGA AATCTCCAGT CTACTGGCAA ACTCGCAGTG CATGCTGAAG
    ATACTCGGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGACGCC TGTCGGTTTG TGACTAAAAT TGTGGGTAAA
    AACCCATTAC TCCTGACAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAGAG TCAACACAAT AATGCTGAAT
    AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAT TTTAAACCCA
    TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAGAGAAG
    ATCTGTCCTC TGCTGGAAAA TACACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA
    GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT
    CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC
    TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT
    TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC
    AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT
    GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG
    AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG
    GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC
    ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC
    ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA
    GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG
    AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC
    CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT
    GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT
    TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA
    CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG
    TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC
    ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA
    AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC
    CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326696.1
    CDS388..7626
    Translation

    Target ORF information:

    RefSeq Version XM_021471021.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471021.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGAGCCAAA ACTTTCGATT AACTTACATT TTAGAAAACA TTGAAGGATC TCAGGTGTGT 
    TTGAGCTGTG ACTTAGTCTT TTTAAAGGTT TCTGGATTCT GTTTCTGCTG CACAAGACCG
    TCTTCTCGAC ATGTAAGATC AGGTTCGTAT GTATCCAGCT CTGTGTCTGT GAAGAGTGAT
    GATTCAAAAA AAGGAGGTCA GCCAAACTTC AGTAGAGAAA CACTGTCACC TACCACAAGT
    GTTCAAAATG AGTCTTCAGA CACAGACATC CAGACTCAGA AAAAACACAT GAGTTTTAAA
    GACAATCTTC AGGGGATCTT CCAGGATCTT GAGAACCAAA TATTAAAATT TCTGAAGAAT
    GAGCTAGAAA AGTTTAAGAA AATATTACAT GAAGAAAATA CAGAATACTT TGTAAAGGAC
    TTTGGTGAGA ACAGATGCAG AATAAAAGAA GCAGCTCTTG ATCTCACACT CTACTTCCTG
    AAAGAAATGA AGCAAGATGA AGCAGCAGAT ACTCTAGAAG ATGAGCTGTT CTTCATTCAT
    CAGCTGAAAT GTGGCCTACA GAAGAAGTAT CAGTGTGTGT TTGAAGGAAT TGCACAGCAA
    GGAGACTCCA CACTCCTGAA GAACATCTAC ACAGATCTCT ATATCACTCA GGGTGGCAGT
    GAACAGGTCA ATACTGAACA TGAGGTCAGG CAGATTGAAG TTACTTCCAG ACGTCATAAA
    GCTCAAGAGA TACAGGTTGA ATGCAGACAT TTGTTTGAAG CGTCTGAACA AGACAAGCAG
    ATCAGAACTG TACTGACAAA AGGAGTCGCT GGCATCGGGA AATCAGTCTC TGTGCAAAAG
    TTTGTTCTGG ATTGGGCTGA AGGAAAAGAA AATCAAGAGA TCAGCTTCAT ATTTCCTCTT
    CCATTCAGAG AGATGAACTT AAAGGAGAAA GAAACACCCA GTTTGATGGA GCTTATAACT
    CAGTTTTACC CAGAGACAAA AGGACTGAAC CTTACAAGAA GGAATCGATT CAAAGTCCTG
    TTCATCCTTG ATGGATTGGA TGAATGTCGT CTTCCTCTGA ACTTTGCTGG TAATGAGACG
    TGTCGTGATG TTTCGTCACC AGCCTCTCTG GATGTTCTCC TGACGAACCT CATCAAGGGA
    AATCTGCTTC CTTCTGCTCT CATCTGGATC ACCAGCAGAC CAGCAGCTGC CAGTAAGATT
    CCTCCTGACT GTATCGACAG AGTGACAGAG ATACGAGGAT TCAATGATGC ACAAAAGGAG
    GAGTACTTCA GAAAAAGACT CACAGATGAG AGTCTGGCCA AAGAAATCAT TGATCATGTT
    AAAAAATCAA AGAGTCTTTT CATCATGTGC CACATCCCAG TCTTCTGCTG GATTTCAGCC
    ACTGTTCTCC AGAACATTTT ACAGAAGAAG AGAAAAAATG TGAAAAACAA TCAGGCTGAT
    GACGCCTCCA AAACACTGCA GGAATCAAAC ACTGAAGACA CTCCCAAGAC TCTGACACAA
    ATGTACACAC ACTTCTTGCA CGTTCAGATC AAGCAGAGCA GACAAAAGTA TGATGGAGAA
    AACAAACCAG ATGTGTCCTG GGATAAAGAC GCCATCCTTT CACTGGGGAA ACTGGCATTT
    CAGCAGCTGC TAAACAACAA CCTGATCTTC TATGAAACAG ACCTGGAAGC CTGTGGTATT
    GACGTCTATA AAGCTGAAAA GTTCTCAGGC ATGTGTACTG AGATCTTTAA GGAGGAAACA
    GGGATTGTTC TTGGTACCAT GTACTGCTTT GTTCACTTGA GTGTTCAAGA GTTTATTGCA
    GCCCTTTATG CACATCTGAT TCTGAACATC AACAATAGAA GTGTGTTTGA TCAAAACACA
    AGAAATAGAA ATGTGTCCAT GATTGATTTG CTGAAGACTG CAGTGGACAA GGCACTTGAG
    AGTGACAATG GACACCTGGA CCTTTTCCTT CGCTTCCTCC TTGGTCTGTC ACTCCAGTCC
    AACAGTGGAA TCTTAAAAGG CCTGTTGACA CAGCAAGACC ACAATAACTG GAGCAAATGG
    AAAATACTTC AGTACTTCTT TAGAACAGTA GATCAGCAGC AAGACCACAA TGACCAGATC
    AAAGAGGAAA TAGTTCATTA CATCAAGCAG AAGTTTGAAG CAAATCTTTC TCTTGAGAGA
    TCCATCAACC TGTTCCACTG TCTGAATGAA CTAAACGATC AAACTCTGGT GAAAGAGATT
    CAGTCTCACC TTAGCAAAGG AAGGTACTCA TCTTCTGACC TTTCACCTGC CCAGTGGTCT
    GCTCTGGTCT TTGTGTTGTT GACATCAGAT GAAGAGATGG AGGAGTTTGA GCTTCAGAAA
    TTCAAGAAAT CAGACGAGTG TCTCATCAGG CTATTAGCTG TCATCAAAAC CTGCAAAAGA
    GCACTGCTCA GTAAGTGTAA TCTAACAGAG GAAAGCTGTT CAGCTCTGGC TTCAGTTCTC
    AGATCAGACT CCAGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGGATTCA
    GGAGTGAAGC TGCTCTCTGA TGGACTGAAG GACAGTAACT GTACACTGGA GAAACTCAGA
    CTTTCAGAGT GCGGTATCAA TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA
    AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACC ATCAGGAGTG
    AAGGAGCTCA CTGATTTACT GAAACCAGAC TATACACTTA AAACACTGAG GTTTTTGAGT
    CCTGCTGCAG AAGAAGTCTT TCAGTTTGTG ACTAAAATTG TGGGTAAAAA CCCGTTACTC
    CTGAAAGAAC TGAATCTGAG TGATCGTGAA CTAGGAGATA CACGAGTGAA TCAACTCTCT
    GCTCTGCTGC AGGATAAACA CTGTAGACTC AACACACTCA TGCTGAATAA TAACAGGATT
    ACAGCAGAAG GTTGTGCTGC TTTGACTTTT GCATTTAATT TAAATCCTTC ACACCTGATA
    GAGCTCAATC TGAGTGGGAA TAAACTAGGA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG
    CTGGAAAATA CACAATGCCA ATTAGAGAAA CTGAGGCTTT CAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA TTTACAGCAG
    GATCCACACT GTACACTGAA GATACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGCCTGT
    CAGTTTTTGA CTGGAGTTGT GGGTAAAAAC CTGTTACTCT TGAAAGAGCT GAATCTGAGT
    GGACATGAAC TAGGACTCTC AGACAGCTTT CAAATGCTTG TTCTTTTACT GCATGATAAA
    CACTGTACGC TCAACACACT GATTCTTTCA GATTGCAGTA TCACAGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAC ATCAAACCCA TCATACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGGAA CTCACTGATT TACTACTGGA TGAACGCTGT
    AGCTTAAAAA TCATCAGGTT TCTGAAAAGT CCTGCTGCAC AAGAGTCATG TGATTTTCTC
    ACTGAAGTTC TGGATGTAAA TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGATAAA
    TTAGGAGATC TGGATGGAGA GAAAATCTCT GCTTTTTTGA TGGACCCTCA TAGCAAAGTG
    GAGAAAATCA AGCTGAATAA TTGTAAGCTG CTTGAGAAAA GCTCATCAGT TTTAGCAACA
    GTTCTGTCTT CCAAAACCAT CCTGAAAGAG CTGAACCTGA ACAACAGTCG TCTGCTGGAC
    TCAGGAGTCA GAGAGATCTG TGAGGGACTG AAGAATCCTG TGTGTGAGCT GAAGATACTC
    AAGCTTTCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT
    TTGAGTCCTG CTGCTGATGA AGGCTGTCAG TTTGTGACTG GAATTGTGGG TAAAAACCCA
    TTACTTCTGA AAGAGCTAAA TCTGAGTGAT CGTGAACTAG GAGACACACG AGTGAATCAA
    CTCTCTGCTC TACTGCAGGA TAAACACTGT AAACTCAACA CACTCATTCT TAGTAAATGT
    GGTGTAACAG TGGAAAGCTG TTCAGCTCTG GCTACAGTCC TGAGATCAGA GACCAGTCTA
    AAAGAGCTTG ACATGAGCAA CAATAATCTG CAGGAATCAG GAGTGAAGAA ACTCCAGAGC
    GGATTAAAAA ACACAAACTG CACACTGGAA AAACTCAGTC TTTTAGACTG CAGTATCAGT
    GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG
    CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGAAGCTCAC TGAGTTACTG
    AAACCAGACT ATACACTGAA GACACTGAGG TTTTTGAGTG ATGATGCAGA TGAAGCCTGT
    ACACATCTGA ATAAAGTTCT GGGTGGAAAC CTGTTACTCT TGAGAGAGCT GAATCTGAGT
    AAACTTGATC TTGGACCATC AGGATTGGAG AAACTTGCTG CTGTACTGAA AGATAAACAC
    TGTAAACTCA ACACACTGAT TCTGAATAAC AGCAGTATGT CAGAAGAAGA TTGTCGTGTT
    TTGGCTGAAG CTTTTAATTC AAACCCATCA AATCTGACAG AGCTGAATTT GAGTGGGACT
    AAACTCACAA ACTCAGGAAT GAAGAGCTTC ACCACTCTGT TTCAAAACCA ACAGTGTAGA
    CTGGAAAAGC TGAAGTTCAG CAGCATGTGT ATTACAGCAG AAGGTTGTGC TGCTTTGGCT
    TCAGCATTTA ACTCAAATCC TTCACACCTG ATAGAGCTCA ATCTGAGTGA GAATAAACTA
    GGAGACTCAG GAGTAACAGA AATCTCCAGT CTACTGGCAA ACTCGCAGTG CATGCTGAAG
    ATACTCGGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGACGCC TGTCGGTTTG TGACTAAAAT TGTGGGTAAA
    AACCCATTAC TCCTGACAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAGAG TCAACACAAT AATGCTGAAT
    AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAT TTTAAACCCA
    TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAGAGAAG
    ATCTGTCCTC TGCTGGAAAA TACACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA
    GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT
    CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC
    TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT
    TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC
    AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT
    GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG
    AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG
    GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC
    ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC
    ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA
    GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG
    AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC
    CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT
    GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT
    TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA
    CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG
    TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC
    ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA
    AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC
    CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146927
    Clone ID Related Accession (Same CDS sequence) XM_021471022.1 , XM_021471023.1
    Accession Version XM_021471022.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCAGAGG AGCGAGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAG ATCAGGTTCG 
    TATGTATCCA GCTCTGTGTC TGTGAAGAGT GATGATTCAA AAAAAGGAGG TCAGCCAAAC
    TTCAGTAGAG AAACACTGTC ACCTACCACA AGTGTTCAAA ATGAGTCTTC AGACACAGAC
    ATCCAGACTC AGAAAAAACA CATGAGTTTT AAAGACAATC TTCAGGGGAT CTTCCAGGAT
    CTTGAGAACC AAATATTAAA ATTTCTGAAG AATGAGCTAG AAAAGTTTAA GAAAATATTA
    CATGAAGAAA ATACAGAATA CTTTGTAAAG GACTTTGGTG AGAACAGATG CAGAATAAAA
    GAAGCAGCTC TTGATCTCAC ACTCTACTTC CTGAAAGAAA TGAAGCAAGA TGAAGCAGCA
    GATACTCTAG AAGATGAGCT GTTCTTCATT CATCAGCTGA AATGTGGCCT ACAGAAGAAG
    TATCAGTGTG TGTTTGAAGG AATTGCACAG CAAGGAGACT CCACACTCCT GAAGAACATC
    TACACAGATC TCTATATCAC TCAGGGTGGC AGTGAACAGG TCAATACTGA ACATGAGGTC
    AGGCAGATTG AAGTTACTTC CAGACGTCAT AAAGCTCAAG AGATACAGGT TGAATGCAGA
    CATTTGTTTG AAGCGTCTGA ACAAGACAAG CAGATCAGAA CTGTACTGAC AAAAGGAGTC
    GCTGGCATCG GGAAATCAGT CTCTGTGCAA AAGTTTGTTC TGGATTGGGC TGAAGGAAAA
    GAAAATCAAG AGATCAGCTT CATATTTCCT CTTCCATTCA GAGAGATGAA CTTAAAGGAG
    AAAGAAACAC CCAGTTTGAT GGAGCTTATA ACTCAGTTTT ACCCAGAGAC AAAAGGACTG
    AACCTTACAA GAAGGAATCG ATTCAAAGTC CTGTTCATCC TTGATGGATT GGATGAATGT
    CGTCTTCCTC TGAACTTTGC TGGTAATGAG ACGTGTCGTG ATGTTTCGTC ACCAGCCTCT
    CTGGATGTTC TCCTGACGAA CCTCATCAAG GGAAATCTGC TTCCTTCTGC TCTCATCTGG
    ATCACCAGCA GACCAGCAGC TGCCAGTAAG ATTCCTCCTG ACTGTATCGA CAGAGTGACA
    GAGATACGAG GATTCAATGA TGCACAAAAG GAGGAGTACT TCAGAAAAAG ACTCACAGAT
    GAGAGTCTGG CCAAAGAAAT CATTGATCAT GTTAAAAAAT CAAAGAGTCT TTTCATCATG
    TGCCACATCC CAGTCTTCTG CTGGATTTCA GCCACTGTTC TCCAGAACAT TTTACAGAAG
    AAGAGAAAAA ATGTGAAAAA CAATCAGGCT GATGACGCCT CCAAAACACT GCAGGAATCA
    AACACTGAAG ACACTCCCAA GACTCTGACA CAAATGTACA CACACTTCTT GCACGTTCAG
    ATCAAGCAGA GCAGACAAAA GTATGATGGA GAAAACAAAC CAGATGTGTC CTGGGATAAA
    GACGCCATCC TTTCACTGGG GAAACTGGCA TTTCAGCAGC TGCTAAACAA CAACCTGATC
    TTCTATGAAA CAGACCTGGA AGCCTGTGGT ATTGACGTCT ATAAAGCTGA AAAGTTCTCA
    GGCATGTGTA CTGAGATCTT TAAGGAGGAA ACAGGGATTG TTCTTGGTAC CATGTACTGC
    TTTGTTCACT TGAGTGTTCA AGAGTTTATT GCAGCCCTTT ATGCACATCT GATTCTGAAC
    ATCAACAATA GAAGTGTGTT TGATCAAAAC ACAAGAAATA GAAATGTGTC CATGATTGAT
    TTGCTGAAGA CTGCAGTGGA CAAGGCACTT GAGAGTGACA ATGGACACCT GGACCTTTTC
    CTTCGCTTCC TCCTTGGTCT GTCACTCCAG TCCAACAGTG GAATCTTAAA AGGCCTGTTG
    ACACAGCAAG ACCACAATAA CTGGAGCAAA TGGAAAATAC TTCAGTACTT CTTTAGAACA
    GTAGATCAGC AGCAAGACCA CAATGACCAG ATCAAAGAGG AAATAGTTCA TTACATCAAG
    CAGAAGTTTG AAGCAAATCT TTCTCTTGAG AGATCCATCA ACCTGTTCCA CTGTCTGAAT
    GAACTAAACG ATCAAACTCT GGTGAAAGAG ATTCAGTCTC ACCTTAGCAA AGGAAGGTAC
    TCATCTTCTG ACCTTTCACC TGCCCAGTGG TCTGCTCTGG TCTTTGTGTT GTTGACATCA
    GATGAAGAGA TGGAGGAGTT TGAGCTTCAG AAATTCAAGA AATCAGACGA GTGTCTCATC
    AGGCTATTAG CTGTCATCAA AACCTGCAAA AGAGCACTGC TCAGTAAGTG TAATCTAACA
    GAGGAAAGCT GTTCAGCTCT GGCTTCAGTT CTCAGATCAG ACTCCAGCAG TCTGAAGGAT
    CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG
    AAGGACAGTA ACTGTACACT GGAGAAACTC AGACTTTCAG AGTGCGGTAT CAATGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACCATCAGGA GTGAAGGAGC TCACTGATTT ACTGAAACCA
    GACTATACAC TTAAAACACT GAGGTTTTTG AGTCCTGCTG CAGAAGAAGT CTTTCAGTTT
    GTGACTAAAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT
    GAACTAGGAG ATACACGAGT GAATCAACTC TCTGCTCTGC TGCAGGATAA ACACTGTAGA
    CTCAACACAC TCATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTGACT
    TTTGCATTTA ATTTAAATCC TTCACACCTG ATAGAGCTCA ATCTGAGTGG GAATAAACTA
    GGAGACTCAG GAGTAGAGAA GATCTGTCCT CTGCTGGAAA ATACACAATG CCAATTAGAG
    AAACTGAGGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGATTTACAG CAGGATCCAC ACTGTACACT GAAGATACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGCC TGTCAGTTTT TGACTGGAGT TGTGGGTAAA
    AACCTGTTAC TCTTGAAAGA GCTGAATCTG AGTGGACATG AACTAGGACT CTCAGACAGC
    TTTCAAATGC TTGTTCTTTT ACTGCATGAT AAACACTGTA CGCTCAACAC ACTGATTCTT
    TCAGATTGCA GTATCACAGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GACATCAAAC
    CCATCATACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG
    GAACTCACTG ATTTACTACT GGATGAACGC TGTAGCTTAA AAATCATCAG GTTTCTGAAA
    AGTCCTGCTG CACAAGAGTC ATGTGATTTT CTCACTGAAG TTCTGGATGT AAATCCATTA
    TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAAATC
    TCTGCTTTTT TGATGGACCC TCATAGCAAA GTGGAGAAAA TCAAGCTGAA TAATTGTAAG
    CTGCTTGAGA AAAGCTCATC AGTTTTAGCA ACAGTTCTGT CTTCCAAAAC CATCCTGAAA
    GAGCTGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAGAT CTGTGAGGGA
    CTGAAGAATC CTGTGTGTGA GCTGAAGATA CTCAAGCTTT CAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA GTTACTGCAG
    GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT
    CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCATTACTTC TGAAAGAGCT AAATCTGAGT
    GATCGTGAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC
    TGTAAACTCA ACACACTCAT TCTTAGTAAA TGTGGTGTAA CAGTGGAAAG CTGTTCAGCT
    CTGGCTACAG TCCTGAGATC AGAGACCAGT CTAAAAGAGC TTGACATGAG CAACAATAAT
    CTGCAGGAAT CAGGAGTGAA GAAACTCCAG AGCGGATTAA AAAACACAAA CTGCACACTG
    GAAAAACTCA GTCTTTTAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGAAGCT CACTGAGTTA CTGAAACCAG ACTATACACT GAAGACACTG
    AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA
    AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG
    GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT
    AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA
    TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC
    TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG
    TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC
    CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC
    AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC
    GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT
    CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT
    AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT
    GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT
    GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA
    TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA
    TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG
    GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG
    GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG
    CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC
    AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA
    GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC
    TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC
    ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT
    GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA
    GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG
    CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG
    GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG
    AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG
    TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC
    AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG
    GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC
    TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG
    AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT
    AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG
    TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA
    ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG
    AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326697.1
    CDS161..7312
    Translation

    Target ORF information:

    RefSeq Version XM_021471022.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471022.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCAGAGG AGCGAGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAG ATCAGGTTCG 
    TATGTATCCA GCTCTGTGTC TGTGAAGAGT GATGATTCAA AAAAAGGAGG TCAGCCAAAC
    TTCAGTAGAG AAACACTGTC ACCTACCACA AGTGTTCAAA ATGAGTCTTC AGACACAGAC
    ATCCAGACTC AGAAAAAACA CATGAGTTTT AAAGACAATC TTCAGGGGAT CTTCCAGGAT
    CTTGAGAACC AAATATTAAA ATTTCTGAAG AATGAGCTAG AAAAGTTTAA GAAAATATTA
    CATGAAGAAA ATACAGAATA CTTTGTAAAG GACTTTGGTG AGAACAGATG CAGAATAAAA
    GAAGCAGCTC TTGATCTCAC ACTCTACTTC CTGAAAGAAA TGAAGCAAGA TGAAGCAGCA
    GATACTCTAG AAGATGAGCT GTTCTTCATT CATCAGCTGA AATGTGGCCT ACAGAAGAAG
    TATCAGTGTG TGTTTGAAGG AATTGCACAG CAAGGAGACT CCACACTCCT GAAGAACATC
    TACACAGATC TCTATATCAC TCAGGGTGGC AGTGAACAGG TCAATACTGA ACATGAGGTC
    AGGCAGATTG AAGTTACTTC CAGACGTCAT AAAGCTCAAG AGATACAGGT TGAATGCAGA
    CATTTGTTTG AAGCGTCTGA ACAAGACAAG CAGATCAGAA CTGTACTGAC AAAAGGAGTC
    GCTGGCATCG GGAAATCAGT CTCTGTGCAA AAGTTTGTTC TGGATTGGGC TGAAGGAAAA
    GAAAATCAAG AGATCAGCTT CATATTTCCT CTTCCATTCA GAGAGATGAA CTTAAAGGAG
    AAAGAAACAC CCAGTTTGAT GGAGCTTATA ACTCAGTTTT ACCCAGAGAC AAAAGGACTG
    AACCTTACAA GAAGGAATCG ATTCAAAGTC CTGTTCATCC TTGATGGATT GGATGAATGT
    CGTCTTCCTC TGAACTTTGC TGGTAATGAG ACGTGTCGTG ATGTTTCGTC ACCAGCCTCT
    CTGGATGTTC TCCTGACGAA CCTCATCAAG GGAAATCTGC TTCCTTCTGC TCTCATCTGG
    ATCACCAGCA GACCAGCAGC TGCCAGTAAG ATTCCTCCTG ACTGTATCGA CAGAGTGACA
    GAGATACGAG GATTCAATGA TGCACAAAAG GAGGAGTACT TCAGAAAAAG ACTCACAGAT
    GAGAGTCTGG CCAAAGAAAT CATTGATCAT GTTAAAAAAT CAAAGAGTCT TTTCATCATG
    TGCCACATCC CAGTCTTCTG CTGGATTTCA GCCACTGTTC TCCAGAACAT TTTACAGAAG
    AAGAGAAAAA ATGTGAAAAA CAATCAGGCT GATGACGCCT CCAAAACACT GCAGGAATCA
    AACACTGAAG ACACTCCCAA GACTCTGACA CAAATGTACA CACACTTCTT GCACGTTCAG
    ATCAAGCAGA GCAGACAAAA GTATGATGGA GAAAACAAAC CAGATGTGTC CTGGGATAAA
    GACGCCATCC TTTCACTGGG GAAACTGGCA TTTCAGCAGC TGCTAAACAA CAACCTGATC
    TTCTATGAAA CAGACCTGGA AGCCTGTGGT ATTGACGTCT ATAAAGCTGA AAAGTTCTCA
    GGCATGTGTA CTGAGATCTT TAAGGAGGAA ACAGGGATTG TTCTTGGTAC CATGTACTGC
    TTTGTTCACT TGAGTGTTCA AGAGTTTATT GCAGCCCTTT ATGCACATCT GATTCTGAAC
    ATCAACAATA GAAGTGTGTT TGATCAAAAC ACAAGAAATA GAAATGTGTC CATGATTGAT
    TTGCTGAAGA CTGCAGTGGA CAAGGCACTT GAGAGTGACA ATGGACACCT GGACCTTTTC
    CTTCGCTTCC TCCTTGGTCT GTCACTCCAG TCCAACAGTG GAATCTTAAA AGGCCTGTTG
    ACACAGCAAG ACCACAATAA CTGGAGCAAA TGGAAAATAC TTCAGTACTT CTTTAGAACA
    GTAGATCAGC AGCAAGACCA CAATGACCAG ATCAAAGAGG AAATAGTTCA TTACATCAAG
    CAGAAGTTTG AAGCAAATCT TTCTCTTGAG AGATCCATCA ACCTGTTCCA CTGTCTGAAT
    GAACTAAACG ATCAAACTCT GGTGAAAGAG ATTCAGTCTC ACCTTAGCAA AGGAAGGTAC
    TCATCTTCTG ACCTTTCACC TGCCCAGTGG TCTGCTCTGG TCTTTGTGTT GTTGACATCA
    GATGAAGAGA TGGAGGAGTT TGAGCTTCAG AAATTCAAGA AATCAGACGA GTGTCTCATC
    AGGCTATTAG CTGTCATCAA AACCTGCAAA AGAGCACTGC TCAGTAAGTG TAATCTAACA
    GAGGAAAGCT GTTCAGCTCT GGCTTCAGTT CTCAGATCAG ACTCCAGCAG TCTGAAGGAT
    CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG
    AAGGACAGTA ACTGTACACT GGAGAAACTC AGACTTTCAG AGTGCGGTAT CAATGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACCATCAGGA GTGAAGGAGC TCACTGATTT ACTGAAACCA
    GACTATACAC TTAAAACACT GAGGTTTTTG AGTCCTGCTG CAGAAGAAGT CTTTCAGTTT
    GTGACTAAAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT
    GAACTAGGAG ATACACGAGT GAATCAACTC TCTGCTCTGC TGCAGGATAA ACACTGTAGA
    CTCAACACAC TCATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTGACT
    TTTGCATTTA ATTTAAATCC TTCACACCTG ATAGAGCTCA ATCTGAGTGG GAATAAACTA
    GGAGACTCAG GAGTAGAGAA GATCTGTCCT CTGCTGGAAA ATACACAATG CCAATTAGAG
    AAACTGAGGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGATTTACAG CAGGATCCAC ACTGTACACT GAAGATACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGCC TGTCAGTTTT TGACTGGAGT TGTGGGTAAA
    AACCTGTTAC TCTTGAAAGA GCTGAATCTG AGTGGACATG AACTAGGACT CTCAGACAGC
    TTTCAAATGC TTGTTCTTTT ACTGCATGAT AAACACTGTA CGCTCAACAC ACTGATTCTT
    TCAGATTGCA GTATCACAGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GACATCAAAC
    CCATCATACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG
    GAACTCACTG ATTTACTACT GGATGAACGC TGTAGCTTAA AAATCATCAG GTTTCTGAAA
    AGTCCTGCTG CACAAGAGTC ATGTGATTTT CTCACTGAAG TTCTGGATGT AAATCCATTA
    TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAAATC
    TCTGCTTTTT TGATGGACCC TCATAGCAAA GTGGAGAAAA TCAAGCTGAA TAATTGTAAG
    CTGCTTGAGA AAAGCTCATC AGTTTTAGCA ACAGTTCTGT CTTCCAAAAC CATCCTGAAA
    GAGCTGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAGAT CTGTGAGGGA
    CTGAAGAATC CTGTGTGTGA GCTGAAGATA CTCAAGCTTT CAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA GTTACTGCAG
    GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT
    CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCATTACTTC TGAAAGAGCT AAATCTGAGT
    GATCGTGAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC
    TGTAAACTCA ACACACTCAT TCTTAGTAAA TGTGGTGTAA CAGTGGAAAG CTGTTCAGCT
    CTGGCTACAG TCCTGAGATC AGAGACCAGT CTAAAAGAGC TTGACATGAG CAACAATAAT
    CTGCAGGAAT CAGGAGTGAA GAAACTCCAG AGCGGATTAA AAAACACAAA CTGCACACTG
    GAAAAACTCA GTCTTTTAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGAAGCT CACTGAGTTA CTGAAACCAG ACTATACACT GAAGACACTG
    AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA
    AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG
    GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT
    AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA
    TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC
    TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG
    TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC
    CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC
    AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC
    GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT
    CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT
    AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT
    GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT
    GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA
    TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA
    TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG
    GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG
    GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG
    CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC
    AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA
    GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC
    TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC
    ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT
    GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA
    GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG
    CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG
    GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG
    AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG
    TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC
    AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG
    GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC
    TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG
    AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT
    AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG
    TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA
    ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG
    AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146927
    Clone ID Related Accession (Same CDS sequence) XM_021471022.1 , XM_021471023.1
    Accession Version XM_021471023.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCAGAGG AGCGAGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAG ATCAGGTTCG 
    TATGTATCCA GCTCTGTGTC TGTGAAGAGT GATGATTCAA AAAAAGGAGG TCAGCCAAAC
    TTCAGTAGAG AAACACTGTC ACCTACCACA AGTGTTCAAA ATGAGTCTTC AGACACAGAC
    ATCCAGACTC AGAAAAAACA CATGAGTTTT AAAGACAATC TTCAGGGGAT CTTCCAGGAT
    CTTGAGAACC AAATATTAAA ATTTCTGAAG AATGAGCTAG AAAAGTTTAA GAAAATATTA
    CATGAAGAAA ATACAGAATA CTTTGTAAAG GACTTTGGTG AGAACAGATG CAGAATAAAA
    GAAGCAGCTC TTGATCTCAC ACTCTACTTC CTGAAAGAAA TGAAGCAAGA TGAAGCAGCA
    GATACTCTAG AAGATGAGCT GTTCTTCATT CATCAGCTGA AATGTGGCCT ACAGAAGAAG
    TATCAGTGTG TGTTTGAAGG AATTGCACAG CAAGGAGACT CCACACTCCT GAAGAACATC
    TACACAGATC TCTATATCAC TCAGGGTGGC AGTGAACAGG TCAATACTGA ACATGAGGTC
    AGGCAGATTG AAGTTACTTC CAGACGTCAT AAAGCTCAAG AGATACAGGT TGAATGCAGA
    CATTTGTTTG AAGCGTCTGA ACAAGACAAG CAGATCAGAA CTGTACTGAC AAAAGGAGTC
    GCTGGCATCG GGAAATCAGT CTCTGTGCAA AAGTTTGTTC TGGATTGGGC TGAAGGAAAA
    GAAAATCAAG AGATCAGCTT CATATTTCCT CTTCCATTCA GAGAGATGAA CTTAAAGGAG
    AAAGAAACAC CCAGTTTGAT GGAGCTTATA ACTCAGTTTT ACCCAGAGAC AAAAGGACTG
    AACCTTACAA GAAGGAATCG ATTCAAAGTC CTGTTCATCC TTGATGGATT GGATGAATGT
    CGTCTTCCTC TGAACTTTGC TGGTAATGAG ACGTGTCGTG ATGTTTCGTC ACCAGCCTCT
    CTGGATGTTC TCCTGACGAA CCTCATCAAG GGAAATCTGC TTCCTTCTGC TCTCATCTGG
    ATCACCAGCA GACCAGCAGC TGCCAGTAAG ATTCCTCCTG ACTGTATCGA CAGAGTGACA
    GAGATACGAG GATTCAATGA TGCACAAAAG GAGGAGTACT TCAGAAAAAG ACTCACAGAT
    GAGAGTCTGG CCAAAGAAAT CATTGATCAT GTTAAAAAAT CAAAGAGTCT TTTCATCATG
    TGCCACATCC CAGTCTTCTG CTGGATTTCA GCCACTGTTC TCCAGAACAT TTTACAGAAG
    AAGAGAAAAA ATGTGAAAAA CAATCAGGCT GATGACGCCT CCAAAACACT GCAGGAATCA
    AACACTGAAG ACACTCCCAA GACTCTGACA CAAATGTACA CACACTTCTT GCACGTTCAG
    ATCAAGCAGA GCAGACAAAA GTATGATGGA GAAAACAAAC CAGATGTGTC CTGGGATAAA
    GACGCCATCC TTTCACTGGG GAAACTGGCA TTTCAGCAGC TGCTAAACAA CAACCTGATC
    TTCTATGAAA CAGACCTGGA AGCCTGTGGT ATTGACGTCT ATAAAGCTGA AAAGTTCTCA
    GGCATGTGTA CTGAGATCTT TAAGGAGGAA ACAGGGATTG TTCTTGGTAC CATGTACTGC
    TTTGTTCACT TGAGTGTTCA AGAGTTTATT GCAGCCCTTT ATGCACATCT GATTCTGAAC
    ATCAACAATA GAAGTGTGTT TGATCAAAAC ACAAGAAATA GAAATGTGTC CATGATTGAT
    TTGCTGAAGA CTGCAGTGGA CAAGGCACTT GAGAGTGACA ATGGACACCT GGACCTTTTC
    CTTCGCTTCC TCCTTGGTCT GTCACTCCAG TCCAACAGTG GAATCTTAAA AGGCCTGTTG
    ACACAGCAAG ACCACAATAA CTGGAGCAAA TGGAAAATAC TTCAGTACTT CTTTAGAACA
    GTAGATCAGC AGCAAGACCA CAATGACCAG ATCAAAGAGG AAATAGTTCA TTACATCAAG
    CAGAAGTTTG AAGCAAATCT TTCTCTTGAG AGATCCATCA ACCTGTTCCA CTGTCTGAAT
    GAACTAAACG ATCAAACTCT GGTGAAAGAG ATTCAGTCTC ACCTTAGCAA AGGAAGGTAC
    TCATCTTCTG ACCTTTCACC TGCCCAGTGG TCTGCTCTGG TCTTTGTGTT GTTGACATCA
    GATGAAGAGA TGGAGGAGTT TGAGCTTCAG AAATTCAAGA AATCAGACGA GTGTCTCATC
    AGGCTATTAG CTGTCATCAA AACCTGCAAA AGAGCACTGC TCAGTAAGTG TAATCTAACA
    GAGGAAAGCT GTTCAGCTCT GGCTTCAGTT CTCAGATCAG ACTCCAGCAG TCTGAAGGAT
    CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG
    AAGGACAGTA ACTGTACACT GGAGAAACTC AGACTTTCAG AGTGCGGTAT CAATGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACCATCAGGA GTGAAGGAGC TCACTGATTT ACTGAAACCA
    GACTATACAC TTAAAACACT GAGGTTTTTG AGTCCTGCTG CAGAAGAAGT CTTTCAGTTT
    GTGACTAAAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT
    GAACTAGGAG ATACACGAGT GAATCAACTC TCTGCTCTGC TGCAGGATAA ACACTGTAGA
    CTCAACACAC TCATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTGACT
    TTTGCATTTA ATTTAAATCC TTCACACCTG ATAGAGCTCA ATCTGAGTGG GAATAAACTA
    GGAGACTCAG GAGTAGAGAA GATCTGTCCT CTGCTGGAAA ATACACAATG CCAATTAGAG
    AAACTGAGGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGATTTACAG CAGGATCCAC ACTGTACACT GAAGATACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGCC TGTCAGTTTT TGACTGGAGT TGTGGGTAAA
    AACCTGTTAC TCTTGAAAGA GCTGAATCTG AGTGGACATG AACTAGGACT CTCAGACAGC
    TTTCAAATGC TTGTTCTTTT ACTGCATGAT AAACACTGTA CGCTCAACAC ACTGATTCTT
    TCAGATTGCA GTATCACAGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GACATCAAAC
    CCATCATACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG
    GAACTCACTG ATTTACTACT GGATGAACGC TGTAGCTTAA AAATCATCAG GTTTCTGAAA
    AGTCCTGCTG CACAAGAGTC ATGTGATTTT CTCACTGAAG TTCTGGATGT AAATCCATTA
    TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAAATC
    TCTGCTTTTT TGATGGACCC TCATAGCAAA GTGGAGAAAA TCAAGCTGAA TAATTGTAAG
    CTGCTTGAGA AAAGCTCATC AGTTTTAGCA ACAGTTCTGT CTTCCAAAAC CATCCTGAAA
    GAGCTGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAGAT CTGTGAGGGA
    CTGAAGAATC CTGTGTGTGA GCTGAAGATA CTCAAGCTTT CAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA GTTACTGCAG
    GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT
    CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCATTACTTC TGAAAGAGCT AAATCTGAGT
    GATCGTGAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC
    TGTAAACTCA ACACACTCAT TCTTAGTAAA TGTGGTGTAA CAGTGGAAAG CTGTTCAGCT
    CTGGCTACAG TCCTGAGATC AGAGACCAGT CTAAAAGAGC TTGACATGAG CAACAATAAT
    CTGCAGGAAT CAGGAGTGAA GAAACTCCAG AGCGGATTAA AAAACACAAA CTGCACACTG
    GAAAAACTCA GTCTTTTAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGAAGCT CACTGAGTTA CTGAAACCAG ACTATACACT GAAGACACTG
    AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA
    AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG
    GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT
    AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA
    TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC
    TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG
    TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC
    CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC
    AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC
    GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT
    CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT
    AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT
    GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT
    GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA
    TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA
    TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG
    GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG
    GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG
    CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC
    AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA
    GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC
    TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC
    ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT
    GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA
    GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG
    CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG
    GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG
    AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG
    TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC
    AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG
    GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC
    TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG
    AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT
    AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG
    TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA
    ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG
    AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326698.1
    CDS178..7329
    Translation

    Target ORF information:

    RefSeq Version XM_021471023.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471023.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGTCAGAGG AGCGAGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAG ATCAGGTTCG 
    TATGTATCCA GCTCTGTGTC TGTGAAGAGT GATGATTCAA AAAAAGGAGG TCAGCCAAAC
    TTCAGTAGAG AAACACTGTC ACCTACCACA AGTGTTCAAA ATGAGTCTTC AGACACAGAC
    ATCCAGACTC AGAAAAAACA CATGAGTTTT AAAGACAATC TTCAGGGGAT CTTCCAGGAT
    CTTGAGAACC AAATATTAAA ATTTCTGAAG AATGAGCTAG AAAAGTTTAA GAAAATATTA
    CATGAAGAAA ATACAGAATA CTTTGTAAAG GACTTTGGTG AGAACAGATG CAGAATAAAA
    GAAGCAGCTC TTGATCTCAC ACTCTACTTC CTGAAAGAAA TGAAGCAAGA TGAAGCAGCA
    GATACTCTAG AAGATGAGCT GTTCTTCATT CATCAGCTGA AATGTGGCCT ACAGAAGAAG
    TATCAGTGTG TGTTTGAAGG AATTGCACAG CAAGGAGACT CCACACTCCT GAAGAACATC
    TACACAGATC TCTATATCAC TCAGGGTGGC AGTGAACAGG TCAATACTGA ACATGAGGTC
    AGGCAGATTG AAGTTACTTC CAGACGTCAT AAAGCTCAAG AGATACAGGT TGAATGCAGA
    CATTTGTTTG AAGCGTCTGA ACAAGACAAG CAGATCAGAA CTGTACTGAC AAAAGGAGTC
    GCTGGCATCG GGAAATCAGT CTCTGTGCAA AAGTTTGTTC TGGATTGGGC TGAAGGAAAA
    GAAAATCAAG AGATCAGCTT CATATTTCCT CTTCCATTCA GAGAGATGAA CTTAAAGGAG
    AAAGAAACAC CCAGTTTGAT GGAGCTTATA ACTCAGTTTT ACCCAGAGAC AAAAGGACTG
    AACCTTACAA GAAGGAATCG ATTCAAAGTC CTGTTCATCC TTGATGGATT GGATGAATGT
    CGTCTTCCTC TGAACTTTGC TGGTAATGAG ACGTGTCGTG ATGTTTCGTC ACCAGCCTCT
    CTGGATGTTC TCCTGACGAA CCTCATCAAG GGAAATCTGC TTCCTTCTGC TCTCATCTGG
    ATCACCAGCA GACCAGCAGC TGCCAGTAAG ATTCCTCCTG ACTGTATCGA CAGAGTGACA
    GAGATACGAG GATTCAATGA TGCACAAAAG GAGGAGTACT TCAGAAAAAG ACTCACAGAT
    GAGAGTCTGG CCAAAGAAAT CATTGATCAT GTTAAAAAAT CAAAGAGTCT TTTCATCATG
    TGCCACATCC CAGTCTTCTG CTGGATTTCA GCCACTGTTC TCCAGAACAT TTTACAGAAG
    AAGAGAAAAA ATGTGAAAAA CAATCAGGCT GATGACGCCT CCAAAACACT GCAGGAATCA
    AACACTGAAG ACACTCCCAA GACTCTGACA CAAATGTACA CACACTTCTT GCACGTTCAG
    ATCAAGCAGA GCAGACAAAA GTATGATGGA GAAAACAAAC CAGATGTGTC CTGGGATAAA
    GACGCCATCC TTTCACTGGG GAAACTGGCA TTTCAGCAGC TGCTAAACAA CAACCTGATC
    TTCTATGAAA CAGACCTGGA AGCCTGTGGT ATTGACGTCT ATAAAGCTGA AAAGTTCTCA
    GGCATGTGTA CTGAGATCTT TAAGGAGGAA ACAGGGATTG TTCTTGGTAC CATGTACTGC
    TTTGTTCACT TGAGTGTTCA AGAGTTTATT GCAGCCCTTT ATGCACATCT GATTCTGAAC
    ATCAACAATA GAAGTGTGTT TGATCAAAAC ACAAGAAATA GAAATGTGTC CATGATTGAT
    TTGCTGAAGA CTGCAGTGGA CAAGGCACTT GAGAGTGACA ATGGACACCT GGACCTTTTC
    CTTCGCTTCC TCCTTGGTCT GTCACTCCAG TCCAACAGTG GAATCTTAAA AGGCCTGTTG
    ACACAGCAAG ACCACAATAA CTGGAGCAAA TGGAAAATAC TTCAGTACTT CTTTAGAACA
    GTAGATCAGC AGCAAGACCA CAATGACCAG ATCAAAGAGG AAATAGTTCA TTACATCAAG
    CAGAAGTTTG AAGCAAATCT TTCTCTTGAG AGATCCATCA ACCTGTTCCA CTGTCTGAAT
    GAACTAAACG ATCAAACTCT GGTGAAAGAG ATTCAGTCTC ACCTTAGCAA AGGAAGGTAC
    TCATCTTCTG ACCTTTCACC TGCCCAGTGG TCTGCTCTGG TCTTTGTGTT GTTGACATCA
    GATGAAGAGA TGGAGGAGTT TGAGCTTCAG AAATTCAAGA AATCAGACGA GTGTCTCATC
    AGGCTATTAG CTGTCATCAA AACCTGCAAA AGAGCACTGC TCAGTAAGTG TAATCTAACA
    GAGGAAAGCT GTTCAGCTCT GGCTTCAGTT CTCAGATCAG ACTCCAGCAG TCTGAAGGAT
    CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG
    AAGGACAGTA ACTGTACACT GGAGAAACTC AGACTTTCAG AGTGCGGTAT CAATGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACCATCAGGA GTGAAGGAGC TCACTGATTT ACTGAAACCA
    GACTATACAC TTAAAACACT GAGGTTTTTG AGTCCTGCTG CAGAAGAAGT CTTTCAGTTT
    GTGACTAAAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT
    GAACTAGGAG ATACACGAGT GAATCAACTC TCTGCTCTGC TGCAGGATAA ACACTGTAGA
    CTCAACACAC TCATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTGACT
    TTTGCATTTA ATTTAAATCC TTCACACCTG ATAGAGCTCA ATCTGAGTGG GAATAAACTA
    GGAGACTCAG GAGTAGAGAA GATCTGTCCT CTGCTGGAAA ATACACAATG CCAATTAGAG
    AAACTGAGGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGATTTACAG CAGGATCCAC ACTGTACACT GAAGATACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGCC TGTCAGTTTT TGACTGGAGT TGTGGGTAAA
    AACCTGTTAC TCTTGAAAGA GCTGAATCTG AGTGGACATG AACTAGGACT CTCAGACAGC
    TTTCAAATGC TTGTTCTTTT ACTGCATGAT AAACACTGTA CGCTCAACAC ACTGATTCTT
    TCAGATTGCA GTATCACAGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GACATCAAAC
    CCATCATACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG
    GAACTCACTG ATTTACTACT GGATGAACGC TGTAGCTTAA AAATCATCAG GTTTCTGAAA
    AGTCCTGCTG CACAAGAGTC ATGTGATTTT CTCACTGAAG TTCTGGATGT AAATCCATTA
    TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAAATC
    TCTGCTTTTT TGATGGACCC TCATAGCAAA GTGGAGAAAA TCAAGCTGAA TAATTGTAAG
    CTGCTTGAGA AAAGCTCATC AGTTTTAGCA ACAGTTCTGT CTTCCAAAAC CATCCTGAAA
    GAGCTGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAGAT CTGTGAGGGA
    CTGAAGAATC CTGTGTGTGA GCTGAAGATA CTCAAGCTTT CAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA GTTACTGCAG
    GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT
    CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCATTACTTC TGAAAGAGCT AAATCTGAGT
    GATCGTGAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC
    TGTAAACTCA ACACACTCAT TCTTAGTAAA TGTGGTGTAA CAGTGGAAAG CTGTTCAGCT
    CTGGCTACAG TCCTGAGATC AGAGACCAGT CTAAAAGAGC TTGACATGAG CAACAATAAT
    CTGCAGGAAT CAGGAGTGAA GAAACTCCAG AGCGGATTAA AAAACACAAA CTGCACACTG
    GAAAAACTCA GTCTTTTAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGAAGCT CACTGAGTTA CTGAAACCAG ACTATACACT GAAGACACTG
    AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA
    AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG
    GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT
    AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA
    TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC
    TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG
    TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC
    CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC
    AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC
    GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT
    CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT
    AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT
    GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT
    GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA
    TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA
    TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG
    GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG
    GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG
    CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC
    AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA
    GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC
    TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC
    ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT
    GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA
    GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG
    CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG
    GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG
    AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG
    TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC
    AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG
    GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC
    TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG
    AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT
    AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG
    TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA
    ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG
    AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146928
    Clone ID Related Accession (Same CDS sequence) XM_021471024.1
    Accession Version XM_021471024.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAGTTCCT CAAAAAAACA CAGTGTAAGA TCAGGCTCAC ATGTGTCCAG CTCTGTGTCT 
    GTGAAGAGTG ACCAGTCAAA AGGTCACATG CCAAACTTCA GTGAGACAAA AACAAAATCA
    AATAAAACGT TTCAATATAA GACATTAGAA TCTGACTTCC ATCCTCAAAG GAGCTGCAAT
    AATTTCAAAG ACCTTCAGTG CATCTTTAAG GGGCTTGAGA GCAAAATGTT CAACTTCCTG
    AAGAAAGAGT TGGAAAATTT TAAGGAAATC TTACGAAAAG AAAACACACA ATACCTTGTA
    AAGGATTTTA ATAAGAACAG ATGCAGCATC AGAGAAACAG CTCTTGATCT TACACTTTAC
    TTCCTGAGAG AAATGAAGCA AGATGAAGCT GCAGATACTC TAGAAGATGA GCTGTTCTTC
    ATTCATCAGC TGAAATGTGG CCTACAGAAG AAGTATCAGT GTGTGTTTGA AGGAATTGCA
    CAGCAAGGAG ACTCCACACT CCTGAAGAAC ATCTACACAG ATCTCTATAT CACTCAGGGT
    GGCAGTGAAC AGGTCAATAC TGAACATGAG GTCAGGCAGA TTGAAGTTAC TTCCAGACGT
    CATAAAGCTC AAGAGATACA GGTTGAATGC AGACATTTGT TTGAAGCGTC TGAACAAGAC
    AAGCAGATCA GAACTGTACT GACAAAAGGA GTCGCTGGCA TCGGGAAATC AGTCTCTGTG
    CAAAAGTTTG TTCTGGATTG GGCTGAAGGA AAAGAAAATC AAGAGATCAG CTTCATATTT
    CCTCTTCCAT TCAGAGAGAT GAACTTAAAG GAGAAAGAAA CACCCAGTTT GATGGAGCTT
    ATAACTCAGT TTTACCCAGA GACAAAAGGA CTGAACCTTA CAAGAAGGAA TCGATTCAAA
    GTCCTGTTCA TCCTTGATGG ATTGGATGAA TGTCGTCTTC CTCTGAACTT TGCTGGTAAT
    GAGACGTGTC GTGATGTTTC GTCACCAGCC TCTCTGGATG TTCTCCTGAC GAACCTCATC
    AAGGGAAATC TGCTTCCTTC TGCTCTCATC TGGATCACCA GCAGACCAGC AGCTGCCAGT
    AAGATTCCTC CTGACTGTAT CGACAGAGTG ACAGAGATAC GAGGATTCAA TGATGCACAA
    AAGGAGGAGT ACTTCAGAAA AAGACTCACA GATGAGAGTC TGGCCAAAGA AATCATTGAT
    CATGTTAAAA AATCAAAGAG TCTTTTCATC ATGTGCCACA TCCCAGTCTT CTGCTGGATT
    TCAGCCACTG TTCTCCAGAA CATTTTACAG AAGAAGAGAA AAAATGTGAA AAACAATCAG
    GCTGATGACG CCTCCAAAAC ACTGCAGGAA TCAAACACTG AAGACACTCC CAAGACTCTG
    ACACAAATGT ACACACACTT CTTGCACGTT CAGATCAAGC AGAGCAGACA AAAGTATGAT
    GGAGAAAACA AACCAGATGT GTCCTGGGAT AAAGACGCCA TCCTTTCACT GGGGAAACTG
    GCATTTCAGC AGCTGCTAAA CAACAACCTG ATCTTCTATG AAACAGACCT GGAAGCCTGT
    GGTATTGACG TCTATAAAGC TGAAAAGTTC TCAGGCATGT GTACTGAGAT CTTTAAGGAG
    GAAACAGGGA TTGTTCTTGG TACCATGTAC TGCTTTGTTC ACTTGAGTGT TCAAGAGTTT
    ATTGCAGCCC TTTATGCACA TCTGATTCTG AACATCAACA ATAGAAGTGT GTTTGATCAA
    AACACAAGAA ATAGAAATGT GTCCATGATT GATTTGCTGA AGACTGCAGT GGACAAGGCA
    CTTGAGAGTG ACAATGGACA CCTGGACCTT TTCCTTCGCT TCCTCCTTGG TCTGTCACTC
    CAGTCCAACA GTGGAATCTT AAAAGGCCTG TTGACACAGC AAGACCACAA TAACTGGAGC
    AAATGGAAAA TACTTCAGTA CTTCTTTAGA ACAGTAGATC AGCAGCAAGA CCACAATGAC
    CAGATCAAAG AGGAAATAGT TCATTACATC AAGCAGAAGT TTGAAGCAAA TCTTTCTCTT
    GAGAGATCCA TCAACCTGTT CCACTGTCTG AATGAACTAA ACGATCAAAC TCTGGTGAAA
    GAGATTCAGT CTCACCTTAG CAAAGGAAGG TACTCATCTT CTGACCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGTCTTTGT GTTGTTGACA TCAGATGAAG AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGGCTAT TAGCTGTCAT CAAAACCTGC
    AAAAGAGCAC TGCTCAGTAA GTGTAATCTA ACAGAGGAAA GCTGTTCAGC TCTGGCTTCA
    GTTCTCAGAT CAGACTCCAG CAGTCTGAAG GATCTTGACC TGAGCAATAA TAATCTGCAG
    GATTCAGGAG TGAAGCTGCT CTCTGATGGA CTGAAGGACA GTAACTGTAC ACTGGAGAAA
    CTCAGACTTT CAGAGTGCGG TATCAATGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG
    AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACCATCA
    GGAGTGAAGG AGCTCACTGA TTTACTGAAA CCAGACTATA CACTTAAAAC ACTGAGGTTT
    TTGAGTCCTG CTGCAGAAGA AGTCTTTCAG TTTGTGACTA AAATTGTGGG TAAAAACCCG
    TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTGAACTAG GAGATACACG AGTGAATCAA
    CTCTCTGCTC TGCTGCAGGA TAAACACTGT AGACTCAACA CACTCATGCT GAATAATAAC
    AGGATTACAG CAGAAGGTTG TGCTGCTTTG ACTTTTGCAT TTAATTTAAA TCCTTCACAC
    CTGATAGAGC TCAATCTGAG TGGGAATAAA CTAGGAGACT CAGGAGTAGA GAAGATCTGT
    CCTCTGCTGG AAAATACACA ATGCCAATTA GAGAAACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG
    TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA
    GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT
    CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT
    AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT
    CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT
    GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG
    TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT
    TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT
    AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG
    CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG
    ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GACGCCTGTC GGTTTGTGAC TAAAATTGTG
    GGTAAAAACC CATTACTCCT GACAGAACTG AATCTGAGTG ATCGTAAACT AGGAGACACA
    CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GTAGAGTCAA CACAATAATG
    CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTATTTTA
    AACCCATCAC ACCTGATAGA GCTCAATCTG AGTGGGAATA AACTAGGAGA CTCAGGAGTA
    GAGAAGATCT GTCCTCTGCT GGAAAATACA CAATGCCGAT TAGAGAAACT GAGTCTTTCA
    GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGAAG
    CTCACTGATT TACTGCACAG TAGAAGCTGT AAATTCAGAT TTCTGAAGAG TTCTGCTGCA
    CAGGAAGCAT GTGACTATCT TACTAAAGTT CTGGGTATAA GTCCATTATT ACTGACAGAA
    CTGGATCTGA GTGAAGATAA ATTAGGAGAT CTGGATGGAG AGAAACTCTC TGCTCTTCTG
    ATGGACTCTC ATAGCAAAGT GAAGAAAATC AAGCTGAATA ATTGTGAGCT GACAGAGAAA
    AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC TCCAAAACCA TCCTGAAAGA GATGAACCTG
    AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAGAAATCT GTGAGGGACT GAAGAATCCT
    GTGTGTGAGC TGAAAATACT CAGTCTTTCA GACTGCCGTA TCAGTGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGACT TACTGGAGGA TCCACACTGT
    ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AAGGCTGTCA CTTTGTGACT
    GAAATGGTGG GTAAAAACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGTACTA
    GGAGACACAC GAGTGAATCA ACTCTCTGCT CTGCTGCAGG ATAAACACTG TCAGATCCAC
    ACACTCATTC TGTGTTATTG CAGTATTACA GAGGAACAGT GTGTGATCCT GACTTCAGCT
    CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGAAACAA TCTAGGAGAC
    TCTGCAGTGA AAAAACTCAG TGATTTACTG ATGAACCAAC AATTCAAGCT GGAGAAACTA
    CGTCTGAGTG ATTGCAGTAT TACAGAGAAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAATGGGA ATAGACTAGG AGACTCTGGA
    GCTTATGGAC TTGGCCATCT GCTGAGCAGT GTGGATTGCA AGCTGAATAA ACTACATCTG
    TGTGATTGCA GTATTACAGG GAAACAGTTG CACTTCCTGA TTGCAGTTAT GTGTATGGAC
    CCATCACACC TGAGGGTACT GGACCTCAGT AAGAATCAAA TAAAAAGCAG AGGAATGAAC
    ATGCTGTGTG ATGTGCTGAA TAAACCACGC TGTAAACTCA AGAGTTTAAG ACTAAATGAC
    TGTGGCATTA CAGATGTTAC ACACTTAACT CAGTCTTTGG CTGAGAACAA AGCACTGGAG
    TTCTTAAAAG AGCTTGAACT GAGTAAAAAT CAAATGGGAG ACTCCGAAAA GCAAAAACTC
    AGTGACCTGC TGCGAGACTC AAACTGTAAA CTGAGGTTAG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326699.1
    CDS281..7405
    Translation

    Target ORF information:

    RefSeq Version XM_021471024.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGAGTTCCT CAAAAAAACA CAGTGTAAGA TCAGGCTCAC ATGTGTCCAG CTCTGTGTCT 
    GTGAAGAGTG ACCAGTCAAA AGGTCACATG CCAAACTTCA GTGAGACAAA AACAAAATCA
    AATAAAACGT TTCAATATAA GACATTAGAA TCTGACTTCC ATCCTCAAAG GAGCTGCAAT
    AATTTCAAAG ACCTTCAGTG CATCTTTAAG GGGCTTGAGA GCAAAATGTT CAACTTCCTG
    AAGAAAGAGT TGGAAAATTT TAAGGAAATC TTACGAAAAG AAAACACACA ATACCTTGTA
    AAGGATTTTA ATAAGAACAG ATGCAGCATC AGAGAAACAG CTCTTGATCT TACACTTTAC
    TTCCTGAGAG AAATGAAGCA AGATGAAGCT GCAGATACTC TAGAAGATGA GCTGTTCTTC
    ATTCATCAGC TGAAATGTGG CCTACAGAAG AAGTATCAGT GTGTGTTTGA AGGAATTGCA
    CAGCAAGGAG ACTCCACACT CCTGAAGAAC ATCTACACAG ATCTCTATAT CACTCAGGGT
    GGCAGTGAAC AGGTCAATAC TGAACATGAG GTCAGGCAGA TTGAAGTTAC TTCCAGACGT
    CATAAAGCTC AAGAGATACA GGTTGAATGC AGACATTTGT TTGAAGCGTC TGAACAAGAC
    AAGCAGATCA GAACTGTACT GACAAAAGGA GTCGCTGGCA TCGGGAAATC AGTCTCTGTG
    CAAAAGTTTG TTCTGGATTG GGCTGAAGGA AAAGAAAATC AAGAGATCAG CTTCATATTT
    CCTCTTCCAT TCAGAGAGAT GAACTTAAAG GAGAAAGAAA CACCCAGTTT GATGGAGCTT
    ATAACTCAGT TTTACCCAGA GACAAAAGGA CTGAACCTTA CAAGAAGGAA TCGATTCAAA
    GTCCTGTTCA TCCTTGATGG ATTGGATGAA TGTCGTCTTC CTCTGAACTT TGCTGGTAAT
    GAGACGTGTC GTGATGTTTC GTCACCAGCC TCTCTGGATG TTCTCCTGAC GAACCTCATC
    AAGGGAAATC TGCTTCCTTC TGCTCTCATC TGGATCACCA GCAGACCAGC AGCTGCCAGT
    AAGATTCCTC CTGACTGTAT CGACAGAGTG ACAGAGATAC GAGGATTCAA TGATGCACAA
    AAGGAGGAGT ACTTCAGAAA AAGACTCACA GATGAGAGTC TGGCCAAAGA AATCATTGAT
    CATGTTAAAA AATCAAAGAG TCTTTTCATC ATGTGCCACA TCCCAGTCTT CTGCTGGATT
    TCAGCCACTG TTCTCCAGAA CATTTTACAG AAGAAGAGAA AAAATGTGAA AAACAATCAG
    GCTGATGACG CCTCCAAAAC ACTGCAGGAA TCAAACACTG AAGACACTCC CAAGACTCTG
    ACACAAATGT ACACACACTT CTTGCACGTT CAGATCAAGC AGAGCAGACA AAAGTATGAT
    GGAGAAAACA AACCAGATGT GTCCTGGGAT AAAGACGCCA TCCTTTCACT GGGGAAACTG
    GCATTTCAGC AGCTGCTAAA CAACAACCTG ATCTTCTATG AAACAGACCT GGAAGCCTGT
    GGTATTGACG TCTATAAAGC TGAAAAGTTC TCAGGCATGT GTACTGAGAT CTTTAAGGAG
    GAAACAGGGA TTGTTCTTGG TACCATGTAC TGCTTTGTTC ACTTGAGTGT TCAAGAGTTT
    ATTGCAGCCC TTTATGCACA TCTGATTCTG AACATCAACA ATAGAAGTGT GTTTGATCAA
    AACACAAGAA ATAGAAATGT GTCCATGATT GATTTGCTGA AGACTGCAGT GGACAAGGCA
    CTTGAGAGTG ACAATGGACA CCTGGACCTT TTCCTTCGCT TCCTCCTTGG TCTGTCACTC
    CAGTCCAACA GTGGAATCTT AAAAGGCCTG TTGACACAGC AAGACCACAA TAACTGGAGC
    AAATGGAAAA TACTTCAGTA CTTCTTTAGA ACAGTAGATC AGCAGCAAGA CCACAATGAC
    CAGATCAAAG AGGAAATAGT TCATTACATC AAGCAGAAGT TTGAAGCAAA TCTTTCTCTT
    GAGAGATCCA TCAACCTGTT CCACTGTCTG AATGAACTAA ACGATCAAAC TCTGGTGAAA
    GAGATTCAGT CTCACCTTAG CAAAGGAAGG TACTCATCTT CTGACCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGTCTTTGT GTTGTTGACA TCAGATGAAG AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGGCTAT TAGCTGTCAT CAAAACCTGC
    AAAAGAGCAC TGCTCAGTAA GTGTAATCTA ACAGAGGAAA GCTGTTCAGC TCTGGCTTCA
    GTTCTCAGAT CAGACTCCAG CAGTCTGAAG GATCTTGACC TGAGCAATAA TAATCTGCAG
    GATTCAGGAG TGAAGCTGCT CTCTGATGGA CTGAAGGACA GTAACTGTAC ACTGGAGAAA
    CTCAGACTTT CAGAGTGCGG TATCAATGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG
    AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACCATCA
    GGAGTGAAGG AGCTCACTGA TTTACTGAAA CCAGACTATA CACTTAAAAC ACTGAGGTTT
    TTGAGTCCTG CTGCAGAAGA AGTCTTTCAG TTTGTGACTA AAATTGTGGG TAAAAACCCG
    TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTGAACTAG GAGATACACG AGTGAATCAA
    CTCTCTGCTC TGCTGCAGGA TAAACACTGT AGACTCAACA CACTCATGCT GAATAATAAC
    AGGATTACAG CAGAAGGTTG TGCTGCTTTG ACTTTTGCAT TTAATTTAAA TCCTTCACAC
    CTGATAGAGC TCAATCTGAG TGGGAATAAA CTAGGAGACT CAGGAGTAGA GAAGATCTGT
    CCTCTGCTGG AAAATACACA ATGCCAATTA GAGAAACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG
    TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA
    GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT
    CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT
    AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT
    CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT
    GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG
    TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT
    TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT
    AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG
    CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG
    ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GACGCCTGTC GGTTTGTGAC TAAAATTGTG
    GGTAAAAACC CATTACTCCT GACAGAACTG AATCTGAGTG ATCGTAAACT AGGAGACACA
    CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GTAGAGTCAA CACAATAATG
    CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTATTTTA
    AACCCATCAC ACCTGATAGA GCTCAATCTG AGTGGGAATA AACTAGGAGA CTCAGGAGTA
    GAGAAGATCT GTCCTCTGCT GGAAAATACA CAATGCCGAT TAGAGAAACT GAGTCTTTCA
    GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGAAG
    CTCACTGATT TACTGCACAG TAGAAGCTGT AAATTCAGAT TTCTGAAGAG TTCTGCTGCA
    CAGGAAGCAT GTGACTATCT TACTAAAGTT CTGGGTATAA GTCCATTATT ACTGACAGAA
    CTGGATCTGA GTGAAGATAA ATTAGGAGAT CTGGATGGAG AGAAACTCTC TGCTCTTCTG
    ATGGACTCTC ATAGCAAAGT GAAGAAAATC AAGCTGAATA ATTGTGAGCT GACAGAGAAA
    AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC TCCAAAACCA TCCTGAAAGA GATGAACCTG
    AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAGAAATCT GTGAGGGACT GAAGAATCCT
    GTGTGTGAGC TGAAAATACT CAGTCTTTCA GACTGCCGTA TCAGTGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGACT TACTGGAGGA TCCACACTGT
    ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AAGGCTGTCA CTTTGTGACT
    GAAATGGTGG GTAAAAACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGTACTA
    GGAGACACAC GAGTGAATCA ACTCTCTGCT CTGCTGCAGG ATAAACACTG TCAGATCCAC
    ACACTCATTC TGTGTTATTG CAGTATTACA GAGGAACAGT GTGTGATCCT GACTTCAGCT
    CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGAAACAA TCTAGGAGAC
    TCTGCAGTGA AAAAACTCAG TGATTTACTG ATGAACCAAC AATTCAAGCT GGAGAAACTA
    CGTCTGAGTG ATTGCAGTAT TACAGAGAAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAATGGGA ATAGACTAGG AGACTCTGGA
    GCTTATGGAC TTGGCCATCT GCTGAGCAGT GTGGATTGCA AGCTGAATAA ACTACATCTG
    TGTGATTGCA GTATTACAGG GAAACAGTTG CACTTCCTGA TTGCAGTTAT GTGTATGGAC
    CCATCACACC TGAGGGTACT GGACCTCAGT AAGAATCAAA TAAAAAGCAG AGGAATGAAC
    ATGCTGTGTG ATGTGCTGAA TAAACCACGC TGTAAACTCA AGAGTTTAAG ACTAAATGAC
    TGTGGCATTA CAGATGTTAC ACACTTAACT CAGTCTTTGG CTGAGAACAA AGCACTGGAG
    TTCTTAAAAG AGCTTGAACT GAGTAAAAAT CAAATGGGAG ACTCCGAAAA GCAAAAACTC
    AGTGACCTGC TGCGAGACTC AAACTGTAAA CTGAGGTTAG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146929
    Clone ID Related Accession (Same CDS sequence) XM_021471025.1
    Accession Version XM_021471025.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG
    ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA
    CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT
    GAAGCCTGTA CACATCTGAA TAAAGTTCTG GGTGGAAACC TGTTACTCTT GAGAGAGCTG
    AATCTGAGTA AACTTGATCT TGGACCATCA GGATTGGAGA AACTTGCTGC TGTACTGAAA
    GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCAGTATGTC AGAAGAAGAT
    TGTCGTGTTT TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGACAGA GCTGAATTTG
    AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CCACTCTGTT TCAAAACCAA
    CAGTGTAGAC TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT
    GCTTTGGCTT CAGCATTTAA CTCAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGAG
    AATAAACTAG GAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCGCAGTGC
    ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGGATCCACA CTGTACACTG
    AAGACACTGA GTCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGAAGCT CACTGATTTA CTGCACAGTA GAAGCTGTAA ATTCAGATTT
    CTGAAGAGTT CTGCTGCACA GGAAGCATGT GACTATCTTA CTAAAGTTCT GGGTATAAGT
    CCATTATTAC TGACAGAACT GGATCTGAGT GAAGATAAAT TAGGAGATCT GGATGGAGAG
    AAACTCTCTG CTCTTCTGAT GGACTCTCAT AGCAAAGTGA AGAAAATCAA GCTGAATAAT
    TGTGAGCTGA CAGAGAAAAG CTGTTCACTT CTAGCTGCAG TTCTCTCCTC CAAAACCATC
    CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAAATCTGT
    GAGGGACTGA AGAATCCTGT GTGTGAGCTG AAAATACTCA GTCTTTCAGA CTGCCGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGACTTA
    CTGGAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GGCTGTCACT TTGTGACTGA AATGGTGGGT AAAAACCCAT TACTCCTGAA AGAACTGAAT
    CTGAGTGATC GTGTACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT GCTGCAGGAT
    AAACACTGTC AGATCCACAC ACTCATTCTG TGTTATTGCA GTATTACAGA GGAACAGTGT
    GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAGT
    GGAAACAATC TAGGAGACTC TGCAGTGAAA AAACTCAGTG ATTTACTGAT GAACCAACAA
    TTCAAGCTGG AGAAACTACG TCTGAGTGAT TGCAGTATTA CAGAGAAACA GTGTGTGATC
    CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAATGGGAAT
    AGACTAGGAG ACTCTGGAGC TTATGGACTT GGCCATCTGC TGAGCAGTGT GGATTGCAAG
    CTGAATAAAC TACATCTGTG TGATTGCAGT ATTACAGGGA AACAGTTGCA CTTCCTGATT
    GCAGTTATGT GTATGGACCC ATCACACCTG AGGGTACTGG ACCTCAGTAA GAATCAAATA
    AAAAGCAGAG GAATGAACAT GCTGTGTGAT GTGCTGAATA AACCACGCTG TAAACTCAAG
    AGTTTAAGAC TAAATGACTG TGGCATTACA GATGTTACAC ACTTAACTCA GTCTTTGGCT
    GAGAACAAAG CACTGGAGTT CTTAAAAGAG CTTGAACTGA GTAAAAATCA AATGGGAGAC
    TCCGAAAAGC AAAAACTCAG TGACCTGCTG CGAGACTCAA ACTGTAAACT GAGGTTAGAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326700.1
    CDS182..7144
    Translation

    Target ORF information:

    RefSeq Version XM_021471025.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471025.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG
    ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA
    CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT
    GAAGCCTGTA CACATCTGAA TAAAGTTCTG GGTGGAAACC TGTTACTCTT GAGAGAGCTG
    AATCTGAGTA AACTTGATCT TGGACCATCA GGATTGGAGA AACTTGCTGC TGTACTGAAA
    GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCAGTATGTC AGAAGAAGAT
    TGTCGTGTTT TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGACAGA GCTGAATTTG
    AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CCACTCTGTT TCAAAACCAA
    CAGTGTAGAC TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT
    GCTTTGGCTT CAGCATTTAA CTCAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGAG
    AATAAACTAG GAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCGCAGTGC
    ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGGATCCACA CTGTACACTG
    AAGACACTGA GTCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGAAGCT CACTGATTTA CTGCACAGTA GAAGCTGTAA ATTCAGATTT
    CTGAAGAGTT CTGCTGCACA GGAAGCATGT GACTATCTTA CTAAAGTTCT GGGTATAAGT
    CCATTATTAC TGACAGAACT GGATCTGAGT GAAGATAAAT TAGGAGATCT GGATGGAGAG
    AAACTCTCTG CTCTTCTGAT GGACTCTCAT AGCAAAGTGA AGAAAATCAA GCTGAATAAT
    TGTGAGCTGA CAGAGAAAAG CTGTTCACTT CTAGCTGCAG TTCTCTCCTC CAAAACCATC
    CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAAATCTGT
    GAGGGACTGA AGAATCCTGT GTGTGAGCTG AAAATACTCA GTCTTTCAGA CTGCCGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGACTTA
    CTGGAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GGCTGTCACT TTGTGACTGA AATGGTGGGT AAAAACCCAT TACTCCTGAA AGAACTGAAT
    CTGAGTGATC GTGTACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT GCTGCAGGAT
    AAACACTGTC AGATCCACAC ACTCATTCTG TGTTATTGCA GTATTACAGA GGAACAGTGT
    GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAGT
    GGAAACAATC TAGGAGACTC TGCAGTGAAA AAACTCAGTG ATTTACTGAT GAACCAACAA
    TTCAAGCTGG AGAAACTACG TCTGAGTGAT TGCAGTATTA CAGAGAAACA GTGTGTGATC
    CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAATGGGAAT
    AGACTAGGAG ACTCTGGAGC TTATGGACTT GGCCATCTGC TGAGCAGTGT GGATTGCAAG
    CTGAATAAAC TACATCTGTG TGATTGCAGT ATTACAGGGA AACAGTTGCA CTTCCTGATT
    GCAGTTATGT GTATGGACCC ATCACACCTG AGGGTACTGG ACCTCAGTAA GAATCAAATA
    AAAAGCAGAG GAATGAACAT GCTGTGTGAT GTGCTGAATA AACCACGCTG TAAACTCAAG
    AGTTTAAGAC TAAATGACTG TGGCATTACA GATGTTACAC ACTTAACTCA GTCTTTGGCT
    GAGAACAAAG CACTGGAGTT CTTAAAAGAG CTTGAACTGA GTAAAAATCA AATGGGAGAC
    TCCGAAAAGC AAAAACTCAG TGACCTGCTG CGAGACTCAA ACTGTAAACT GAGGTTAGAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146930
    Clone ID Related Accession (Same CDS sequence) XM_021471026.1
    Accession Version XM_021471026.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTGATGA TGCAGATGAA GCCTGTACAC ATCTGAATAA AGTTCTGGGT
    GGAAACCTGT TACTCTTGAG AGAGCTGAAT CTGAGTAAAC TTGATCTTGG ACCATCAGGA
    TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT AAACACTGTA AACTCAACAC ACTGATTCTG
    AATAACAGCA GTATGTCAGA AGAAGATTGT CGTGTTTTGG CTGAAGCTTT TAATTCAAAC
    CCATCAAATC TGACAGAGCT GAATTTGAGT GGGACTAAAC TCACAAACTC AGGAATGAAG
    AGCTTCACCA CTCTGTTTCA AAACCAACAG TGTAGACTGG AAAAGCTGAA GTTCAGCAGC
    ATGTGTATTA CAGCAGAAGG TTGTGCTGCT TTGGCTTCAG CATTTAACTC AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGAGAAT AAACTAGGAG ACTCAGGAGT AACAGAAATC
    TCCAGTCTAC TGGCAAACTC GCAGTGCATG CTGAAGATAC TCGGTCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GACGCCTGTC GGTTTGTGAC TAAAATTGTG GGTAAAAACC CATTACTCCT GACAGAACTG
    AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG
    GATAAACACT GTAGAGTCAA CACAATAATG CTGAATAATA ACAGGATTAC AGCAGAAGGT
    TGTGCTGCTT TAACTTCAGC ATTTATTTTA AACCCATCAC ACCTGATAGA GCTCAATCTG
    AGTGGGAATA AACTAGGAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATACA
    CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGAAG CTCACTGATT TACTGCACAG TAGAAGCTGT
    AAATTCAGAT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GTGACTATCT TACTAAAGTT
    CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT
    CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GAAGAAAATC
    AAGCTGAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC
    TCCAAAACCA TCCTGAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC
    AGAGAAATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAAATACT CAGTCTTTCA
    GACTGCCGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG
    CTCACTGACT TACTGGAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT
    GCTGCTGATG AAGGCTGTCA CTTTGTGACT GAAATGGTGG GTAAAAACCC ATTACTCCTG
    AAAGAACTGA ATCTGAGTGA TCGTGTACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT
    CTGCTGCAGG ATAAACACTG TCAGATCCAC ACACTCATTC TGTGTTATTG CAGTATTACA
    GAGGAACAGT GTGTGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA
    CTGAACCTCA GTGGAAACAA TCTAGGAGAC TCTGCAGTGA AAAAACTCAG TGATTTACTG
    ATGAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTG ATTGCAGTAT TACAGAGAAA
    CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC
    CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTTATGGAC TTGGCCATCT GCTGAGCAGT
    GTGGATTGCA AGCTGAATAA ACTACATCTG TGTGATTGCA GTATTACAGG GAAACAGTTG
    CACTTCCTGA TTGCAGTTAT GTGTATGGAC CCATCACACC TGAGGGTACT GGACCTCAGT
    AAGAATCAAA TAAAAAGCAG AGGAATGAAC ATGCTGTGTG ATGTGCTGAA TAAACCACGC
    TGTAAACTCA AGAGTTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACACTTAACT
    CAGTCTTTGG CTGAGAACAA AGCACTGGAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT
    CAAATGGGAG ACTCCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA
    CTGAGGTTAG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326701.1
    CDS182..6976
    Translation

    Target ORF information:

    RefSeq Version XM_021471026.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTGATGA TGCAGATGAA GCCTGTACAC ATCTGAATAA AGTTCTGGGT
    GGAAACCTGT TACTCTTGAG AGAGCTGAAT CTGAGTAAAC TTGATCTTGG ACCATCAGGA
    TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT AAACACTGTA AACTCAACAC ACTGATTCTG
    AATAACAGCA GTATGTCAGA AGAAGATTGT CGTGTTTTGG CTGAAGCTTT TAATTCAAAC
    CCATCAAATC TGACAGAGCT GAATTTGAGT GGGACTAAAC TCACAAACTC AGGAATGAAG
    AGCTTCACCA CTCTGTTTCA AAACCAACAG TGTAGACTGG AAAAGCTGAA GTTCAGCAGC
    ATGTGTATTA CAGCAGAAGG TTGTGCTGCT TTGGCTTCAG CATTTAACTC AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGAGAAT AAACTAGGAG ACTCAGGAGT AACAGAAATC
    TCCAGTCTAC TGGCAAACTC GCAGTGCATG CTGAAGATAC TCGGTCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GACGCCTGTC GGTTTGTGAC TAAAATTGTG GGTAAAAACC CATTACTCCT GACAGAACTG
    AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG
    GATAAACACT GTAGAGTCAA CACAATAATG CTGAATAATA ACAGGATTAC AGCAGAAGGT
    TGTGCTGCTT TAACTTCAGC ATTTATTTTA AACCCATCAC ACCTGATAGA GCTCAATCTG
    AGTGGGAATA AACTAGGAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATACA
    CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGAAG CTCACTGATT TACTGCACAG TAGAAGCTGT
    AAATTCAGAT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GTGACTATCT TACTAAAGTT
    CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT
    CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GAAGAAAATC
    AAGCTGAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC
    TCCAAAACCA TCCTGAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC
    AGAGAAATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAAATACT CAGTCTTTCA
    GACTGCCGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG
    CTCACTGACT TACTGGAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT
    GCTGCTGATG AAGGCTGTCA CTTTGTGACT GAAATGGTGG GTAAAAACCC ATTACTCCTG
    AAAGAACTGA ATCTGAGTGA TCGTGTACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT
    CTGCTGCAGG ATAAACACTG TCAGATCCAC ACACTCATTC TGTGTTATTG CAGTATTACA
    GAGGAACAGT GTGTGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA
    CTGAACCTCA GTGGAAACAA TCTAGGAGAC TCTGCAGTGA AAAAACTCAG TGATTTACTG
    ATGAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTG ATTGCAGTAT TACAGAGAAA
    CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC
    CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTTATGGAC TTGGCCATCT GCTGAGCAGT
    GTGGATTGCA AGCTGAATAA ACTACATCTG TGTGATTGCA GTATTACAGG GAAACAGTTG
    CACTTCCTGA TTGCAGTTAT GTGTATGGAC CCATCACACC TGAGGGTACT GGACCTCAGT
    AAGAATCAAA TAAAAAGCAG AGGAATGAAC ATGCTGTGTG ATGTGCTGAA TAAACCACGC
    TGTAAACTCA AGAGTTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACACTTAACT
    CAGTCTTTGG CTGAGAACAA AGCACTGGAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT
    CAAATGGGAG ACTCCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA
    CTGAGGTTAG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146931
    Clone ID Related Accession (Same CDS sequence) XM_021471028.1
    Accession Version XM_021471028.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product protein NLRC5 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGATTTACAG CAGGATCCAC ACTGTACACT GAAGATACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGCC TGTCAGTTTT TGACTGGAGT TGTGGGTAAA
    AACCTGTTAC TCTTGAAAGA GCTGAATCTG AGTGGACATG AACTAGGACT CTCAGACAGC
    TTTCAAATGC TTGTTCTTTT ACTGCATGAT AAACACTGTA CGCTCAACAC ACTGATTCTT
    TCAGATTGCA GTATCACAGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GACATCAAAC
    CCATCATACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG
    GAACTCACTG ATTTACTACT GGATGAACGC TGTAGCTTAA AAATCATCAG GTTTCTGAAA
    AGTCCTGCTG CACAAGAGTC ATGTGATTTT CTCACTGAAG TTCTGGATGT AAATCCATTA
    TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAAATC
    TCTGCTTTTT TGATGGACCC TCATAGCAAA GTGGAGAAAA TCAAGCTGAA TAATTGTAAG
    CTGCTTGAGA AAAGCTCATC AGTTTTAGCA ACAGTTCTGT CTTCCAAAAC CATCCTGAAA
    GAGCTGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAGAT CTGTGAGGGA
    CTGAAGAATC CTGTGTGTGA GCTGAAGATA CTCAAGCTTT CAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA GTTACTGCAG
    GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT
    CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCATTACTTC TGAAAGAGCT AAATCTGAGT
    GATCGTGAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC
    TGTAAACTCA ACACACTCAT TCTTAGTAAA TGTGGTGTAA CAGTGGAAAG CTGTTCAGCT
    CTGGCTACAG TCCTGAGATC AGAGACCAGT CTAAAAGAGC TTGACATGAG CAACAATAAT
    CTGCAGGAAT CAGGAGTGAA GAAACTCCAG AGCGGATTAA AAAACACAAA CTGCACACTG
    GAAAAACTCA GTCTTTTAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGAAGCT CACTGAGTTA CTGAAACCAG ACTATACACT GAAGACACTG
    AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA
    AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG
    GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT
    AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA
    TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC
    TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG
    TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC
    CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC
    AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC
    GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT
    CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT
    AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT
    GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT
    GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA
    TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA
    TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG
    GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG
    GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG
    CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC
    AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA
    GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC
    TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC
    ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT
    GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA
    GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG
    CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG
    GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG
    AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG
    TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC
    AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG
    GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC
    TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG
    AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT
    AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG
    TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA
    ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG
    AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326703.1
    CDS182..6973
    Translation

    Target ORF information:

    RefSeq Version XM_021471028.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471028.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGATTTACAG CAGGATCCAC ACTGTACACT GAAGATACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGCC TGTCAGTTTT TGACTGGAGT TGTGGGTAAA
    AACCTGTTAC TCTTGAAAGA GCTGAATCTG AGTGGACATG AACTAGGACT CTCAGACAGC
    TTTCAAATGC TTGTTCTTTT ACTGCATGAT AAACACTGTA CGCTCAACAC ACTGATTCTT
    TCAGATTGCA GTATCACAGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GACATCAAAC
    CCATCATACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG
    GAACTCACTG ATTTACTACT GGATGAACGC TGTAGCTTAA AAATCATCAG GTTTCTGAAA
    AGTCCTGCTG CACAAGAGTC ATGTGATTTT CTCACTGAAG TTCTGGATGT AAATCCATTA
    TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAAATC
    TCTGCTTTTT TGATGGACCC TCATAGCAAA GTGGAGAAAA TCAAGCTGAA TAATTGTAAG
    CTGCTTGAGA AAAGCTCATC AGTTTTAGCA ACAGTTCTGT CTTCCAAAAC CATCCTGAAA
    GAGCTGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAGAT CTGTGAGGGA
    CTGAAGAATC CTGTGTGTGA GCTGAAGATA CTCAAGCTTT CAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA GTTACTGCAG
    GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT
    CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCATTACTTC TGAAAGAGCT AAATCTGAGT
    GATCGTGAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC
    TGTAAACTCA ACACACTCAT TCTTAGTAAA TGTGGTGTAA CAGTGGAAAG CTGTTCAGCT
    CTGGCTACAG TCCTGAGATC AGAGACCAGT CTAAAAGAGC TTGACATGAG CAACAATAAT
    CTGCAGGAAT CAGGAGTGAA GAAACTCCAG AGCGGATTAA AAAACACAAA CTGCACACTG
    GAAAAACTCA GTCTTTTAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGAAGCT CACTGAGTTA CTGAAACCAG ACTATACACT GAAGACACTG
    AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA
    AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG
    GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT
    AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA
    TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC
    TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG
    TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC
    CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC
    AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC
    GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT
    CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT
    AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT
    GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT
    GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA
    TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA
    TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG
    GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG
    GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG
    CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC
    AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA
    GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC
    TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC
    ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT
    GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA
    GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG
    CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG
    GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG
    AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG
    TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC
    AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG
    GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC
    TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG
    AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT
    AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG
    TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA
    ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG
    AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146932
    Clone ID Related Accession (Same CDS sequence) XM_021471029.1
    Accession Version XM_021471029.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG
    ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA
    CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGTC TTTCAGACTG CAGTATCAGT
    GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG
    CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG
    CAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGACGCC
    TGTCGGTTTG TGACTAAAAT TGTGGGTAAA AACCCATTAC TCCTGACAGA ACTGAATCTG
    AGTGATCGTA AACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA
    CACTGTAGAG TCAACACAAT AATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT
    GCTTTAACTT CAGCATTTAT TTTAAACCCA TCACACCTGA TAGAGCTCAA TCTGAGTGGG
    AATAAACTAG GAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TACACAATGC
    CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GAAGCTCACT GATTTACTGC ACAGTAGAAG CTGTAAATTC
    AGATTTCTGA AGAGTTCTGC TGCACAGGAA GCATGTGACT ATCTTACTAA AGTTCTGGGT
    ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT
    GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGAAGAA AATCAAGCTG
    AATAATTGTG AGCTGACAGA GAAAAGCTGT TCACTTCTAG CTGCAGTTCT CTCCTCCAAA
    ACCATCCTGA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAGAA
    ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAAA TACTCAGTCT TTCAGACTGC
    CGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT
    GACTTACTGG AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT
    GATGAAGGCT GTCACTTTGT GACTGAAATG GTGGGTAAAA ACCCATTACT CCTGAAAGAA
    CTGAATCTGA GTGATCGTGT ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTGCTG
    CAGGATAAAC ACTGTCAGAT CCACACACTC ATTCTGTGTT ATTGCAGTAT TACAGAGGAA
    CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC
    CTCAGTGGAA ACAATCTAGG AGACTCTGCA GTGAAAAAAC TCAGTGATTT ACTGATGAAC
    CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTGATTGCA GTATTACAGA GAAACAGTGT
    GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT
    GGGAATAGAC TAGGAGACTC TGGAGCTTAT GGACTTGGCC ATCTGCTGAG CAGTGTGGAT
    TGCAAGCTGA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGGGAAACA GTTGCACTTC
    CTGATTGCAG TTATGTGTAT GGACCCATCA CACCTGAGGG TACTGGACCT CAGTAAGAAT
    CAAATAAAAA GCAGAGGAAT GAACATGCTG TGTGATGTGC TGAATAAACC ACGCTGTAAA
    CTCAAGAGTT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACACTT AACTCAGTCT
    TTGGCTGAGA ACAAAGCACT GGAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG
    GGAGACTCCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG
    TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326704.1
    CDS182..6970
    Translation

    Target ORF information:

    RefSeq Version XM_021471029.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471029.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG
    ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA
    CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGTC TTTCAGACTG CAGTATCAGT
    GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG
    CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG
    CAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGACGCC
    TGTCGGTTTG TGACTAAAAT TGTGGGTAAA AACCCATTAC TCCTGACAGA ACTGAATCTG
    AGTGATCGTA AACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA
    CACTGTAGAG TCAACACAAT AATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT
    GCTTTAACTT CAGCATTTAT TTTAAACCCA TCACACCTGA TAGAGCTCAA TCTGAGTGGG
    AATAAACTAG GAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TACACAATGC
    CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GAAGCTCACT GATTTACTGC ACAGTAGAAG CTGTAAATTC
    AGATTTCTGA AGAGTTCTGC TGCACAGGAA GCATGTGACT ATCTTACTAA AGTTCTGGGT
    ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT
    GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGAAGAA AATCAAGCTG
    AATAATTGTG AGCTGACAGA GAAAAGCTGT TCACTTCTAG CTGCAGTTCT CTCCTCCAAA
    ACCATCCTGA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAGAA
    ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAAA TACTCAGTCT TTCAGACTGC
    CGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT
    GACTTACTGG AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT
    GATGAAGGCT GTCACTTTGT GACTGAAATG GTGGGTAAAA ACCCATTACT CCTGAAAGAA
    CTGAATCTGA GTGATCGTGT ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTGCTG
    CAGGATAAAC ACTGTCAGAT CCACACACTC ATTCTGTGTT ATTGCAGTAT TACAGAGGAA
    CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC
    CTCAGTGGAA ACAATCTAGG AGACTCTGCA GTGAAAAAAC TCAGTGATTT ACTGATGAAC
    CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTGATTGCA GTATTACAGA GAAACAGTGT
    GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT
    GGGAATAGAC TAGGAGACTC TGGAGCTTAT GGACTTGGCC ATCTGCTGAG CAGTGTGGAT
    TGCAAGCTGA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGGGAAACA GTTGCACTTC
    CTGATTGCAG TTATGTGTAT GGACCCATCA CACCTGAGGG TACTGGACCT CAGTAAGAAT
    CAAATAAAAA GCAGAGGAAT GAACATGCTG TGTGATGTGC TGAATAAACC ACGCTGTAAA
    CTCAAGAGTT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACACTT AACTCAGTCT
    TTGGCTGAGA ACAAAGCACT GGAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG
    GGAGACTCCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG
    TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146933
    Clone ID Related Accession (Same CDS sequence) XM_021471030.1
    Accession Version XM_021471030.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG
    ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA
    CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTCAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT
    GATTTACTGC AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT
    GATGACGCCT GTCGGTTTGT GACTAAAATT GTGGGTAAAA ACCCATTACT CCTGACAGAA
    CTGAATCTGA GTGATCGTAA ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG
    CAGGATAAAC ACTGTAGAGT CAACACAATA ATGCTGAATA ATAACAGGAT TACAGCAGAA
    GGTTGTGCTG CTTTAACTTC AGCATTTATT TTAAACCCAT CACACCTGAT AGAGCTCAAT
    CTGAGTGGGA ATAAACTAGG AGACTCAGGA GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT
    ACACAATGCC GATTAGAGAA ACTGAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT
    AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA
    GGAAATGATC CTGGACAATC AGGAGTGAAG AAGCTCACTG ATTTACTGCA CAGTAGAAGC
    TGTAAATTCA GATTTCTGAA GAGTTCTGCT GCACAGGAAG CATGTGACTA TCTTACTAAA
    GTTCTGGGTA TAAGTCCATT ATTACTGACA GAACTGGATC TGAGTGAAGA TAAATTAGGA
    GATCTGGATG GAGAGAAACT CTCTGCTCTT CTGATGGACT CTCATAGCAA AGTGAAGAAA
    ATCAAGCTGA ATAATTGTGA GCTGACAGAG AAAAGCTGTT CACTTCTAGC TGCAGTTCTC
    TCCTCCAAAA CCATCCTGAA AGAGATGAAC CTGAACAACA GTCGTCTGCT GGACTCAGGA
    GTCAGAGAAA TCTGTGAGGG ACTGAAGAAT CCTGTGTGTG AGCTGAAAAT ACTCAGTCTT
    TCAGACTGCC GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC
    CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG
    GAGCTCACTG ACTTACTGGA GGATCCACAC TGTACACTGA AGACACTGAG GTTTTTGAGT
    CCTGCTGCTG ATGAAGGCTG TCACTTTGTG ACTGAAATGG TGGGTAAAAA CCCATTACTC
    CTGAAAGAAC TGAATCTGAG TGATCGTGTA CTAGGAGACA CACGAGTGAA TCAACTCTCT
    GCTCTGCTGC AGGATAAACA CTGTCAGATC CACACACTCA TTCTGTGTTA TTGCAGTATT
    ACAGAGGAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA
    GAACTGAACC TCAGTGGAAA CAATCTAGGA GACTCTGCAG TGAAAAAACT CAGTGATTTA
    CTGATGAACC AACAATTCAA GCTGGAGAAA CTACGTCTGA GTGATTGCAG TATTACAGAG
    AAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAATG GGAATAGACT AGGAGACTCT GGAGCTTATG GACTTGGCCA TCTGCTGAGC
    AGTGTGGATT GCAAGCTGAA TAAACTACAT CTGTGTGATT GCAGTATTAC AGGGAAACAG
    TTGCACTTCC TGATTGCAGT TATGTGTATG GACCCATCAC ACCTGAGGGT ACTGGACCTC
    AGTAAGAATC AAATAAAAAG CAGAGGAATG AACATGCTGT GTGATGTGCT GAATAAACCA
    CGCTGTAAAC TCAAGAGTTT AAGACTAAAT GACTGTGGCA TTACAGATGT TACACACTTA
    ACTCAGTCTT TGGCTGAGAA CAAAGCACTG GAGTTCTTAA AAGAGCTTGA ACTGAGTAAA
    AATCAAATGG GAGACTCCGA AAAGCAAAAA CTCAGTGACC TGCTGCGAGA CTCAAACTGT
    AAACTGAGGT TAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326705.1
    CDS182..6799
    Translation

    Target ORF information:

    RefSeq Version XM_021471030.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471030.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG
    ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA
    CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTCAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT
    GATTTACTGC AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT
    GATGACGCCT GTCGGTTTGT GACTAAAATT GTGGGTAAAA ACCCATTACT CCTGACAGAA
    CTGAATCTGA GTGATCGTAA ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG
    CAGGATAAAC ACTGTAGAGT CAACACAATA ATGCTGAATA ATAACAGGAT TACAGCAGAA
    GGTTGTGCTG CTTTAACTTC AGCATTTATT TTAAACCCAT CACACCTGAT AGAGCTCAAT
    CTGAGTGGGA ATAAACTAGG AGACTCAGGA GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT
    ACACAATGCC GATTAGAGAA ACTGAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT
    AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA
    GGAAATGATC CTGGACAATC AGGAGTGAAG AAGCTCACTG ATTTACTGCA CAGTAGAAGC
    TGTAAATTCA GATTTCTGAA GAGTTCTGCT GCACAGGAAG CATGTGACTA TCTTACTAAA
    GTTCTGGGTA TAAGTCCATT ATTACTGACA GAACTGGATC TGAGTGAAGA TAAATTAGGA
    GATCTGGATG GAGAGAAACT CTCTGCTCTT CTGATGGACT CTCATAGCAA AGTGAAGAAA
    ATCAAGCTGA ATAATTGTGA GCTGACAGAG AAAAGCTGTT CACTTCTAGC TGCAGTTCTC
    TCCTCCAAAA CCATCCTGAA AGAGATGAAC CTGAACAACA GTCGTCTGCT GGACTCAGGA
    GTCAGAGAAA TCTGTGAGGG ACTGAAGAAT CCTGTGTGTG AGCTGAAAAT ACTCAGTCTT
    TCAGACTGCC GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC
    CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG
    GAGCTCACTG ACTTACTGGA GGATCCACAC TGTACACTGA AGACACTGAG GTTTTTGAGT
    CCTGCTGCTG ATGAAGGCTG TCACTTTGTG ACTGAAATGG TGGGTAAAAA CCCATTACTC
    CTGAAAGAAC TGAATCTGAG TGATCGTGTA CTAGGAGACA CACGAGTGAA TCAACTCTCT
    GCTCTGCTGC AGGATAAACA CTGTCAGATC CACACACTCA TTCTGTGTTA TTGCAGTATT
    ACAGAGGAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA
    GAACTGAACC TCAGTGGAAA CAATCTAGGA GACTCTGCAG TGAAAAAACT CAGTGATTTA
    CTGATGAACC AACAATTCAA GCTGGAGAAA CTACGTCTGA GTGATTGCAG TATTACAGAG
    AAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAATG GGAATAGACT AGGAGACTCT GGAGCTTATG GACTTGGCCA TCTGCTGAGC
    AGTGTGGATT GCAAGCTGAA TAAACTACAT CTGTGTGATT GCAGTATTAC AGGGAAACAG
    TTGCACTTCC TGATTGCAGT TATGTGTATG GACCCATCAC ACCTGAGGGT ACTGGACCTC
    AGTAAGAATC AAATAAAAAG CAGAGGAATG AACATGCTGT GTGATGTGCT GAATAAACCA
    CGCTGTAAAC TCAAGAGTTT AAGACTAAAT GACTGTGGCA TTACAGATGT TACACACTTA
    ACTCAGTCTT TGGCTGAGAA CAAAGCACTG GAGTTCTTAA AAGAGCTTGA ACTGAGTAAA
    AATCAAATGG GAGACTCCGA AAAGCAAAAA CTCAGTGACC TGCTGCGAGA CTCAAACTGT
    AAACTGAGGT TAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146934
    Clone ID Related Accession (Same CDS sequence) XM_021471031.1
    Accession Version XM_021471031.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC
    CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG
    GAGCTCACTG ATTTACTGCA GGATCCACAC TGTACACTGA AGACACTGAG GTTTTTGAGT
    CCTGCTGCTG ATGACGCCTG TCGGTTTGTG ACTAAAATTG TGGGTAAAAA CCCATTACTC
    CTGACAGAAC TGAATCTGAG TGATCGTAAA CTAGGAGACA CACGAGTGAA TCAACTCTCT
    GCTCTACTGC AGGATAAACA CTGTAGAGTC AACACAATAA TGCTGAATAA TAACAGGATT
    ACAGCAGAAG GTTGTGCTGC TTTAACTTCA GCATTTATTT TAAACCCATC ACACCTGATA
    GAGCTCAATC TGAGTGGGAA TAAACTAGGA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG
    CTGGAAAATA CACAATGCCG ATTAGAGAAA CTGAGTCTTT CAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGA AGCTCACTGA TTTACTGCAC
    AGTAGAAGCT GTAAATTCAG ATTTCTGAAG AGTTCTGCTG CACAGGAAGC ATGTGACTAT
    CTTACTAAAG TTCTGGGTAT AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT
    AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGCAAA
    GTGAAGAAAA TCAAGCTGAA TAATTGTGAG CTGACAGAGA AAAGCTGTTC ACTTCTAGCT
    GCAGTTCTCT CCTCCAAAAC CATCCTGAAA GAGATGAACC TGAACAACAG TCGTCTGCTG
    GACTCAGGAG TCAGAGAAAT CTGTGAGGGA CTGAAGAATC CTGTGTGTGA GCTGAAAATA
    CTCAGTCTTT CAGACTGCCG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG
    AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA
    GGAGTGAAGG AGCTCACTGA CTTACTGGAG GATCCACACT GTACACTGAA GACACTGAGG
    TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT CACTTTGTGA CTGAAATGGT GGGTAAAAAC
    CCATTACTCC TGAAAGAACT GAATCTGAGT GATCGTGTAC TAGGAGACAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTCAGATCC ACACACTCAT TCTGTGTTAT
    TGCAGTATTA CAGAGGAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA
    CACCTGAGAG AACTGAACCT CAGTGGAAAC AATCTAGGAG ACTCTGCAGT GAAAAAACTC
    AGTGATTTAC TGATGAACCA ACAATTCAAG CTGGAGAAAC TACGTCTGAG TGATTGCAGT
    ATTACAGAGA AACAGTGTGT GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG
    AGAGAACTGA ACCTCAATGG GAATAGACTA GGAGACTCTG GAGCTTATGG ACTTGGCCAT
    CTGCTGAGCA GTGTGGATTG CAAGCTGAAT AAACTACATC TGTGTGATTG CAGTATTACA
    GGGAAACAGT TGCACTTCCT GATTGCAGTT ATGTGTATGG ACCCATCACA CCTGAGGGTA
    CTGGACCTCA GTAAGAATCA AATAAAAAGC AGAGGAATGA ACATGCTGTG TGATGTGCTG
    AATAAACCAC GCTGTAAACT CAAGAGTTTA AGACTAAATG ACTGTGGCAT TACAGATGTT
    ACACACTTAA CTCAGTCTTT GGCTGAGAAC AAAGCACTGG AGTTCTTAAA AGAGCTTGAA
    CTGAGTAAAA ATCAAATGGG AGACTCCGAA AAGCAAAAAC TCAGTGACCT GCTGCGAGAC
    TCAAACTGTA AACTGAGGTT AGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326706.1
    CDS182..6628
    Translation

    Target ORF information:

    RefSeq Version XM_021471031.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471031.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC
    CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG
    GAGCTCACTG ATTTACTGCA GGATCCACAC TGTACACTGA AGACACTGAG GTTTTTGAGT
    CCTGCTGCTG ATGACGCCTG TCGGTTTGTG ACTAAAATTG TGGGTAAAAA CCCATTACTC
    CTGACAGAAC TGAATCTGAG TGATCGTAAA CTAGGAGACA CACGAGTGAA TCAACTCTCT
    GCTCTACTGC AGGATAAACA CTGTAGAGTC AACACAATAA TGCTGAATAA TAACAGGATT
    ACAGCAGAAG GTTGTGCTGC TTTAACTTCA GCATTTATTT TAAACCCATC ACACCTGATA
    GAGCTCAATC TGAGTGGGAA TAAACTAGGA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG
    CTGGAAAATA CACAATGCCG ATTAGAGAAA CTGAGTCTTT CAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGA AGCTCACTGA TTTACTGCAC
    AGTAGAAGCT GTAAATTCAG ATTTCTGAAG AGTTCTGCTG CACAGGAAGC ATGTGACTAT
    CTTACTAAAG TTCTGGGTAT AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT
    AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGCAAA
    GTGAAGAAAA TCAAGCTGAA TAATTGTGAG CTGACAGAGA AAAGCTGTTC ACTTCTAGCT
    GCAGTTCTCT CCTCCAAAAC CATCCTGAAA GAGATGAACC TGAACAACAG TCGTCTGCTG
    GACTCAGGAG TCAGAGAAAT CTGTGAGGGA CTGAAGAATC CTGTGTGTGA GCTGAAAATA
    CTCAGTCTTT CAGACTGCCG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG
    AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA
    GGAGTGAAGG AGCTCACTGA CTTACTGGAG GATCCACACT GTACACTGAA GACACTGAGG
    TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT CACTTTGTGA CTGAAATGGT GGGTAAAAAC
    CCATTACTCC TGAAAGAACT GAATCTGAGT GATCGTGTAC TAGGAGACAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTCAGATCC ACACACTCAT TCTGTGTTAT
    TGCAGTATTA CAGAGGAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA
    CACCTGAGAG AACTGAACCT CAGTGGAAAC AATCTAGGAG ACTCTGCAGT GAAAAAACTC
    AGTGATTTAC TGATGAACCA ACAATTCAAG CTGGAGAAAC TACGTCTGAG TGATTGCAGT
    ATTACAGAGA AACAGTGTGT GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG
    AGAGAACTGA ACCTCAATGG GAATAGACTA GGAGACTCTG GAGCTTATGG ACTTGGCCAT
    CTGCTGAGCA GTGTGGATTG CAAGCTGAAT AAACTACATC TGTGTGATTG CAGTATTACA
    GGGAAACAGT TGCACTTCCT GATTGCAGTT ATGTGTATGG ACCCATCACA CCTGAGGGTA
    CTGGACCTCA GTAAGAATCA AATAAAAAGC AGAGGAATGA ACATGCTGTG TGATGTGCTG
    AATAAACCAC GCTGTAAACT CAAGAGTTTA AGACTAAATG ACTGTGGCAT TACAGATGTT
    ACACACTTAA CTCAGTCTTT GGCTGAGAAC AAAGCACTGG AGTTCTTAAA AGAGCTTGAA
    CTGAGTAAAA ATCAAATGGG AGACTCCGAA AAGCAAAAAC TCAGTGACCT GCTGCGAGAC
    TCAAACTGTA AACTGAGGTT AGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146935
    Clone ID Related Accession (Same CDS sequence) XM_021471032.1
    Accession Version XM_021471032.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG
    ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA
    CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT
    GAAGCCTGTA CACATCTGAA TAAAGTTCTG GGTGGAAACC TGTTACTCTT GAGAGAGCTG
    AATCTGAGTA AACTTGATCT TGGACCATCA GGATTGGAGA AACTTGCTGC TGTACTGAAA
    GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCAGTATGTC AGAAGAAGAT
    TGTCGTGTTT TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGACAGA GCTGAATTTG
    AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CCACTCTGTT TCAAAACCAA
    CAGTGTAGAC TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT
    GCTTTGGCTT CAGCATTTAA CTCAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGAG
    AATAAACTAG GAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCGCAGTGC
    ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGGATCCACA CTGTACACTG
    AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG
    GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC
    ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC
    ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA
    GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG
    AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC
    CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT
    GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT
    TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA
    CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG
    TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC
    ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA
    AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC
    CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326707.1
    CDS182..6460
    Translation

    Target ORF information:

    RefSeq Version XM_021471032.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471032.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG
    ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA
    CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT
    GAAGCCTGTA CACATCTGAA TAAAGTTCTG GGTGGAAACC TGTTACTCTT GAGAGAGCTG
    AATCTGAGTA AACTTGATCT TGGACCATCA GGATTGGAGA AACTTGCTGC TGTACTGAAA
    GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCAGTATGTC AGAAGAAGAT
    TGTCGTGTTT TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGACAGA GCTGAATTTG
    AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CCACTCTGTT TCAAAACCAA
    CAGTGTAGAC TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT
    GCTTTGGCTT CAGCATTTAA CTCAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGAG
    AATAAACTAG GAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCGCAGTGC
    ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGGATCCACA CTGTACACTG
    AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG
    GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC
    ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC
    ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA
    GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG
    AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC
    CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT
    GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT
    TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA
    CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG
    TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC
    ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA
    AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC
    CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146936
    Clone ID Related Accession (Same CDS sequence) XM_021471033.1
    Accession Version XM_021471033.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATGCTG
    AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTAA CTTCAGCATT TATTTTAAAC
    CCATCACACC TGATAGAGCT CAATCTGAGT GGGAATAAAC TAGGAGACTC AGGAGTAGAG
    AAGATCTGTC CTCTGCTGGA AAATACACAA TGCCGATTAG AGAAACTGAG TCTTTCAGAC
    TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGAAGCTC
    ACTGATTTAC TGCACAGTAG AAGCTGTAAA TTCAGATTTC TGAAGAGTTC TGCTGCACAG
    GAAGCATGTG ACTATCTTAC TAAAGTTCTG GGTATAAGTC CATTATTACT GACAGAACTG
    GATCTGAGTG AAGATAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGATG
    GACTCTCATA GCAAAGTGAA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC
    TGTTCACTTC TAGCTGCAGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC
    AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAAATCTGTG AGGGACTGAA GAATCCTGTG
    TGTGAGCTGA AAATACTCAG TCTTTCAGAC TGCCGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGGAGCTC ACTGACTTAC TGGAGGATCC ACACTGTACA
    CTGAAGACAC TGAGGTTTTT GAGTCCTGCT GCTGATGAAG GCTGTCACTT TGTGACTGAA
    ATGGTGGGTA AAAACCCATT ACTCCTGAAA GAACTGAATC TGAGTGATCG TGTACTAGGA
    GACACACGAG TGAATCAACT CTCTGCTCTG CTGCAGGATA AACACTGTCA GATCCACACA
    CTCATTCTGT GTTATTGCAG TATTACAGAG GAACAGTGTG TGATCCTGAC TTCAGCTCTG
    AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAGTG GAAACAATCT AGGAGACTCT
    GCAGTGAAAA AACTCAGTGA TTTACTGATG AACCAACAAT TCAAGCTGGA GAAACTACGT
    CTGAGTGATT GCAGTATTAC AGAGAAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAACCTC AATGGGAATA GACTAGGAGA CTCTGGAGCT
    TATGGACTTG GCCATCTGCT GAGCAGTGTG GATTGCAAGC TGAATAAACT ACATCTGTGT
    GATTGCAGTA TTACAGGGAA ACAGTTGCAC TTCCTGATTG CAGTTATGTG TATGGACCCA
    TCACACCTGA GGGTACTGGA CCTCAGTAAG AATCAAATAA AAAGCAGAGG AATGAACATG
    CTGTGTGATG TGCTGAATAA ACCACGCTGT AAACTCAAGA GTTTAAGACT AAATGACTGT
    GGCATTACAG ATGTTACACA CTTAACTCAG TCTTTGGCTG AGAACAAAGC ACTGGAGTTC
    TTAAAAGAGC TTGAACTGAG TAAAAATCAA ATGGGAGACT CCGAAAAGCA AAAACTCAGT
    GACCTGCTGC GAGACTCAAA CTGTAAACTG AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326708.1
    CDS182..6283
    Translation

    Target ORF information:

    RefSeq Version XM_021471033.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATGCTG
    AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTAA CTTCAGCATT TATTTTAAAC
    CCATCACACC TGATAGAGCT CAATCTGAGT GGGAATAAAC TAGGAGACTC AGGAGTAGAG
    AAGATCTGTC CTCTGCTGGA AAATACACAA TGCCGATTAG AGAAACTGAG TCTTTCAGAC
    TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGAAGCTC
    ACTGATTTAC TGCACAGTAG AAGCTGTAAA TTCAGATTTC TGAAGAGTTC TGCTGCACAG
    GAAGCATGTG ACTATCTTAC TAAAGTTCTG GGTATAAGTC CATTATTACT GACAGAACTG
    GATCTGAGTG AAGATAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGATG
    GACTCTCATA GCAAAGTGAA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC
    TGTTCACTTC TAGCTGCAGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC
    AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAAATCTGTG AGGGACTGAA GAATCCTGTG
    TGTGAGCTGA AAATACTCAG TCTTTCAGAC TGCCGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGGAGCTC ACTGACTTAC TGGAGGATCC ACACTGTACA
    CTGAAGACAC TGAGGTTTTT GAGTCCTGCT GCTGATGAAG GCTGTCACTT TGTGACTGAA
    ATGGTGGGTA AAAACCCATT ACTCCTGAAA GAACTGAATC TGAGTGATCG TGTACTAGGA
    GACACACGAG TGAATCAACT CTCTGCTCTG CTGCAGGATA AACACTGTCA GATCCACACA
    CTCATTCTGT GTTATTGCAG TATTACAGAG GAACAGTGTG TGATCCTGAC TTCAGCTCTG
    AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAGTG GAAACAATCT AGGAGACTCT
    GCAGTGAAAA AACTCAGTGA TTTACTGATG AACCAACAAT TCAAGCTGGA GAAACTACGT
    CTGAGTGATT GCAGTATTAC AGAGAAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAACCTC AATGGGAATA GACTAGGAGA CTCTGGAGCT
    TATGGACTTG GCCATCTGCT GAGCAGTGTG GATTGCAAGC TGAATAAACT ACATCTGTGT
    GATTGCAGTA TTACAGGGAA ACAGTTGCAC TTCCTGATTG CAGTTATGTG TATGGACCCA
    TCACACCTGA GGGTACTGGA CCTCAGTAAG AATCAAATAA AAAGCAGAGG AATGAACATG
    CTGTGTGATG TGCTGAATAA ACCACGCTGT AAACTCAAGA GTTTAAGACT AAATGACTGT
    GGCATTACAG ATGTTACACA CTTAACTCAG TCTTTGGCTG AGAACAAAGC ACTGGAGTTC
    TTAAAAGAGC TTGAACTGAG TAAAAATCAA ATGGGAGACT CCGAAAAGCA AAAACTCAGT
    GACCTGCTGC GAGACTCAAA CTGTAAACTG AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146937
    Clone ID Related Accession (Same CDS sequence) XM_021471034.1
    Accession Version XM_021471034.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA ATCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGATTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAC GCCTGTCGGT TTGTGACTAA AATTGTGGGT
    AAAAACCCAT TACTCCTGAC AGAACTGAAT CTGAGTGATC GTAAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA GAGTCAACAC AATAATGCTG
    AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTAA CTTCAGCATT TATTTTAAAC
    CCATCACACC TGATAGAGCT CAATCTGAGT GGGAATAAAC TAGGAGACTC AGGAGTAGAG
    AAGATCTGTC CTCTGCTGGA AAATACACAA TGCCGATTAG AGAAACTGAG TCTTTCAGAC
    TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGAAGCTC
    ACTGATTTAC TGCACAGTAG AAGCTGTAAA TTCAGATTTC TGAAGAGTTC TGCTGCACAG
    GAAGCATGTG ACTATCTTAC TAAAGTTCTG GGTATAAGTC CATTATTACT GACAGAACTG
    GATCTGAGTG AAGATAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGATG
    GACTCTCATA GCAAAGTGAA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC
    TGTTCACTTC TAGCTGCAGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC
    AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAAATCTGTG AGGGACTGAA GAATCCTGTG
    TGTGAGCTGA AAATACTCAG TCTTTCAGAC TGCCGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGGAGCTC ACTGACTTAC TGGAGGATCC ACACTGTACA
    CTGAAGACAC TGAGGTTTTT GAGTCCTGCT GCTGATGAAG GCTGTCACTT TGTGACTGAA
    ATGGTGGGTA AAAACCCATT ACTCCTGAAA GAACTGAATC TGAGTGATCG TGTACTAGGA
    GACACACGAG TGAATCAACT CTCTGCTCTG CTGCAGGATA AACACTGTCA GATCCACACA
    CTCATTCTGT GTTATTGCAG TATTACAGAG GAACAGTGTG TGATCCTGAC TTCAGCTCTG
    AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAGTG GAAACAATCT AGGAGACTCT
    GCAGTGAAAA AACTCAGTGA TTTACTGATG AACCAACAAT TCAAGCTGGA GAAACTACGT
    CTGAGTGATT GCAGTATTAC AGAGAAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAACCTC AATGGGAATA GACTAGGAGA CTCTGGAGCT
    TATGGACTTG GCCATCTGCT GAGCAGTGTG GATTGCAAGC TGAATAAACT ACATCTGTGT
    GATTGCAGTA TTACAGGGAA ACAGTTGCAC TTCCTGATTG CAGTTATGTG TATGGACCCA
    TCACACCTGA GGGTACTGGA CCTCAGTAAG AATCAAATAA AAAGCAGAGG AATGAACATG
    CTGTGTGATG TGCTGAATAA ACCACGCTGT AAACTCAAGA GTTTAAGACT AAATGACTGT
    GGCATTACAG ATGTTACACA CTTAACTCAG TCTTTGGCTG AGAACAAAGC ACTGGAGTTC
    TTAAAAGAGC TTGAACTGAG TAAAAATCAA ATGGGAGACT CCGAAAAGCA AAAACTCAGT
    GACCTGCTGC GAGACTCAAA CTGTAAACTG AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326709.1
    CDS182..6283
    Translation

    Target ORF information:

    RefSeq Version XM_021471034.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471034.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA ATCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGATTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAC GCCTGTCGGT TTGTGACTAA AATTGTGGGT
    AAAAACCCAT TACTCCTGAC AGAACTGAAT CTGAGTGATC GTAAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA GAGTCAACAC AATAATGCTG
    AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTAA CTTCAGCATT TATTTTAAAC
    CCATCACACC TGATAGAGCT CAATCTGAGT GGGAATAAAC TAGGAGACTC AGGAGTAGAG
    AAGATCTGTC CTCTGCTGGA AAATACACAA TGCCGATTAG AGAAACTGAG TCTTTCAGAC
    TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGAAGCTC
    ACTGATTTAC TGCACAGTAG AAGCTGTAAA TTCAGATTTC TGAAGAGTTC TGCTGCACAG
    GAAGCATGTG ACTATCTTAC TAAAGTTCTG GGTATAAGTC CATTATTACT GACAGAACTG
    GATCTGAGTG AAGATAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGATG
    GACTCTCATA GCAAAGTGAA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC
    TGTTCACTTC TAGCTGCAGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC
    AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAAATCTGTG AGGGACTGAA GAATCCTGTG
    TGTGAGCTGA AAATACTCAG TCTTTCAGAC TGCCGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGGAGCTC ACTGACTTAC TGGAGGATCC ACACTGTACA
    CTGAAGACAC TGAGGTTTTT GAGTCCTGCT GCTGATGAAG GCTGTCACTT TGTGACTGAA
    ATGGTGGGTA AAAACCCATT ACTCCTGAAA GAACTGAATC TGAGTGATCG TGTACTAGGA
    GACACACGAG TGAATCAACT CTCTGCTCTG CTGCAGGATA AACACTGTCA GATCCACACA
    CTCATTCTGT GTTATTGCAG TATTACAGAG GAACAGTGTG TGATCCTGAC TTCAGCTCTG
    AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAGTG GAAACAATCT AGGAGACTCT
    GCAGTGAAAA AACTCAGTGA TTTACTGATG AACCAACAAT TCAAGCTGGA GAAACTACGT
    CTGAGTGATT GCAGTATTAC AGAGAAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAACCTC AATGGGAATA GACTAGGAGA CTCTGGAGCT
    TATGGACTTG GCCATCTGCT GAGCAGTGTG GATTGCAAGC TGAATAAACT ACATCTGTGT
    GATTGCAGTA TTACAGGGAA ACAGTTGCAC TTCCTGATTG CAGTTATGTG TATGGACCCA
    TCACACCTGA GGGTACTGGA CCTCAGTAAG AATCAAATAA AAAGCAGAGG AATGAACATG
    CTGTGTGATG TGCTGAATAA ACCACGCTGT AAACTCAAGA GTTTAAGACT AAATGACTGT
    GGCATTACAG ATGTTACACA CTTAACTCAG TCTTTGGCTG AGAACAAAGC ACTGGAGTTC
    TTAAAAGAGC TTGAACTGAG TAAAAATCAA ATGGGAGACT CCGAAAAGCA AAAACTCAGT
    GACCTGCTGC GAGACTCAAA CTGTAAACTG AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146938
    Clone ID Related Accession (Same CDS sequence) XM_021471035.1
    Accession Version XM_021471035.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6099bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG
    ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA
    CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTCAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA
    GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT
    CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC
    TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT
    TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC
    AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT
    GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG
    AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG
    GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC
    ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC
    ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA
    GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG
    AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC
    CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT
    GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT
    TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA
    CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG
    TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC
    ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA
    AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC
    CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326710.1
    CDS182..6280
    Translation

    Target ORF information:

    RefSeq Version XM_021471035.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471035.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT
    AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT
    AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG
    ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA
    CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTCAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA
    GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT
    CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC
    TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT
    TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC
    AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT
    GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG
    AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG
    GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC
    ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC
    ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA
    GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG
    AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC
    CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT
    GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT
    TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA
    CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG
    TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC
    ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA
    AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC
    CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146939
    Clone ID Related Accession (Same CDS sequence) XM_021471036.1
    Accession Version XM_021471036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTAACTTCA
    GCATTTATTT TAAACCCATC ACACCTGATA GAGCTCAATC TGAGTGGGAA TAAACTAGGA
    GACTCAGGAG TAGAGAAGAT CTGTCCTCTG CTGGAAAATA CACAATGCCG ATTAGAGAAA
    CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG
    AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA
    GGAGTGAAGA AGCTCACTGA TTTACTGCAC AGTAGAAGCT GTAAATTCAG ATTTCTGAAG
    AGTTCTGCTG CACAGGAAGC ATGTGACTAT CTTACTAAAG TTCTGGGTAT AAGTCCATTA
    TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAACTC
    TCTGCTCTTC TGATGGACTC TCATAGCAAA GTGAAGAAAA TCAAGCTGAA TAATTGTGAG
    CTGACAGAGA AAAGCTGTTC ACTTCTAGCT GCAGTTCTCT CCTCCAAAAC CATCCTGAAA
    GAGATGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAAAT CTGTGAGGGA
    CTGAAGAATC CTGTGTGTGA GCTGAAAATA CTCAGTCTTT CAGACTGCCG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA CTTACTGGAG
    GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT
    CACTTTGTGA CTGAAATGGT GGGTAAAAAC CCATTACTCC TGAAAGAACT GAATCTGAGT
    GATCGTGTAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTGCTGCA GGATAAACAC
    TGTCAGATCC ACACACTCAT TCTGTGTTAT TGCAGTATTA CAGAGGAACA GTGTGTGATC
    CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAGTGGAAAC
    AATCTAGGAG ACTCTGCAGT GAAAAAACTC AGTGATTTAC TGATGAACCA ACAATTCAAG
    CTGGAGAAAC TACGTCTGAG TGATTGCAGT ATTACAGAGA AACAGTGTGT GATCCTGACT
    TCAGCTCTGA AATCAAACCC ATCACACCTG AGAGAACTGA ACCTCAATGG GAATAGACTA
    GGAGACTCTG GAGCTTATGG ACTTGGCCAT CTGCTGAGCA GTGTGGATTG CAAGCTGAAT
    AAACTACATC TGTGTGATTG CAGTATTACA GGGAAACAGT TGCACTTCCT GATTGCAGTT
    ATGTGTATGG ACCCATCACA CCTGAGGGTA CTGGACCTCA GTAAGAATCA AATAAAAAGC
    AGAGGAATGA ACATGCTGTG TGATGTGCTG AATAAACCAC GCTGTAAACT CAAGAGTTTA
    AGACTAAATG ACTGTGGCAT TACAGATGTT ACACACTTAA CTCAGTCTTT GGCTGAGAAC
    AAAGCACTGG AGTTCTTAAA AGAGCTTGAA CTGAGTAAAA ATCAAATGGG AGACTCCGAA
    AAGCAAAAAC TCAGTGACCT GCTGCGAGAC TCAAACTGTA AACTGAGGTT AGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326711.1
    CDS182..5938
    Translation

    Target ORF information:

    RefSeq Version XM_021471036.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT
    TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAGATACTCA AGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTAACTTCA
    GCATTTATTT TAAACCCATC ACACCTGATA GAGCTCAATC TGAGTGGGAA TAAACTAGGA
    GACTCAGGAG TAGAGAAGAT CTGTCCTCTG CTGGAAAATA CACAATGCCG ATTAGAGAAA
    CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG
    AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA
    GGAGTGAAGA AGCTCACTGA TTTACTGCAC AGTAGAAGCT GTAAATTCAG ATTTCTGAAG
    AGTTCTGCTG CACAGGAAGC ATGTGACTAT CTTACTAAAG TTCTGGGTAT AAGTCCATTA
    TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAACTC
    TCTGCTCTTC TGATGGACTC TCATAGCAAA GTGAAGAAAA TCAAGCTGAA TAATTGTGAG
    CTGACAGAGA AAAGCTGTTC ACTTCTAGCT GCAGTTCTCT CCTCCAAAAC CATCCTGAAA
    GAGATGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAAAT CTGTGAGGGA
    CTGAAGAATC CTGTGTGTGA GCTGAAAATA CTCAGTCTTT CAGACTGCCG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA CTTACTGGAG
    GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT
    CACTTTGTGA CTGAAATGGT GGGTAAAAAC CCATTACTCC TGAAAGAACT GAATCTGAGT
    GATCGTGTAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTGCTGCA GGATAAACAC
    TGTCAGATCC ACACACTCAT TCTGTGTTAT TGCAGTATTA CAGAGGAACA GTGTGTGATC
    CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAGTGGAAAC
    AATCTAGGAG ACTCTGCAGT GAAAAAACTC AGTGATTTAC TGATGAACCA ACAATTCAAG
    CTGGAGAAAC TACGTCTGAG TGATTGCAGT ATTACAGAGA AACAGTGTGT GATCCTGACT
    TCAGCTCTGA AATCAAACCC ATCACACCTG AGAGAACTGA ACCTCAATGG GAATAGACTA
    GGAGACTCTG GAGCTTATGG ACTTGGCCAT CTGCTGAGCA GTGTGGATTG CAAGCTGAAT
    AAACTACATC TGTGTGATTG CAGTATTACA GGGAAACAGT TGCACTTCCT GATTGCAGTT
    ATGTGTATGG ACCCATCACA CCTGAGGGTA CTGGACCTCA GTAAGAATCA AATAAAAAGC
    AGAGGAATGA ACATGCTGTG TGATGTGCTG AATAAACCAC GCTGTAAACT CAAGAGTTTA
    AGACTAAATG ACTGTGGCAT TACAGATGTT ACACACTTAA CTCAGTCTTT GGCTGAGAAC
    AAAGCACTGG AGTTCTTAAA AGAGCTTGAA CTGAGTAAAA ATCAAATGGG AGACTCCGAA
    AAGCAAAAAC TCAGTGACCT GCTGCGAGAC TCAAACTGTA AACTGAGGTT AGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146940
    Clone ID Related Accession (Same CDS sequence) XM_021471037.1
    Accession Version XM_021471037.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NLR family CARD domain-containing protein 3 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTG ATGATGCAGA TGAAGCCTGT ACACATCTGA ATAAAGTTCT GGGTGGAAAC
    CTGTTACTCT TGAGAGAGCT GAATCTGAGT AAACTTGATC TTGGACCATC AGGATTGGAG
    AAACTTGCTG CTGTACTGAA AGATAAACAC TGTAAACTCA ACACACTGAT TCTGAATAAC
    AGCAGTATGT CAGAAGAAGA TTGTCGTGTT TTGGCTGAAG CTTTTAATTC AAACCCATCA
    AATCTGACAG AGCTGAATTT GAGTGGGACT AAACTCACAA ACTCAGGAAT GAAGAGCTTC
    ACCACTCTGT TTCAAAACCA ACAGTGTAGA CTGGAAAAGC TGAAGTTCAG CAGCATGTGT
    ATTACAGCAG AAGGTTGTGC TGCTTTGGCT TCAGCATTTA ACTCAAATCC TTCACACCTG
    ATAGAGCTCA ATCTGAGTGA GAATAAACTA GGAGACTCAG GAGTAACAGA AATCTCCAGT
    CTACTGGCAA ACTCGCAGTG CATGCTGAAG ATACTCGGTC TTTCAGACTG CAGTATCAGT
    GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG
    CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG
    CAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGACGCC
    TGTCGGTTTG TGACTAAAAT TGTGGGTAAA AACCCATTAC TCCTGACAGA ACTGAATCTG
    AGTGATCGTA AACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA
    CACTGTAGAG TCAACACAAT AATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT
    GCTTTAACTT CAGCATTTAT TTTAAACCCA TCACACCTGA TAGAGCTCAA TCTGAGTGGG
    AATAAACTAG GAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TACACAATGC
    CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GAAGCTCACT GATTTACTGC ACAGTAGAAG CTGTAAATTC
    AGATTTCTGA AGAGTTCTGC TGCACAGGAA GCATGTGACT ATCTTACTAA AGTTCTGGGT
    ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT
    GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGAAGAA AATCAAGCTG
    AATAATTGTG AGCTGACAGA GAAAAGCTGT TCACTTCTAG CTGCAGTTCT CTCCTCCAAA
    ACCATCCTGA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAGAA
    ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAAA TACTCAGTCT TTCAGACTGC
    CGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT
    GACTTACTGG AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT
    GATGAAGGCT GTCACTTTGT GACTGAAATG GTGGGTAAAA ACCCATTACT CCTGAAAGAA
    CTGAATCTGA GTGATCGTGT ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTGCTG
    CAGGATAAAC ACTGTCAGAT CCACACACTC ATTCTGTGTT ATTGCAGTAT TACAGAGGAA
    CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC
    CTCAGTGGAA ACAATCTAGG AGACTCTGCA GTGAAAAAAC TCAGTGATTT ACTGATGAAC
    CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTGATTGCA GTATTACAGA GAAACAGTGT
    GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT
    GGGAATAGAC TAGGAGACTC TGGAGCTTAT GGACTTGGCC ATCTGCTGAG CAGTGTGGAT
    TGCAAGCTGA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGGGAAACA GTTGCACTTC
    CTGATTGCAG TTATGTGTAT GGACCCATCA CACCTGAGGG TACTGGACCT CAGTAAGAAT
    CAAATAAAAA GCAGAGGAAT GAACATGCTG TGTGATGTGC TGAATAAACC ACGCTGTAAA
    CTCAAGAGTT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACACTT AACTCAGTCT
    TTGGCTGAGA ACAAAGCACT GGAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG
    GGAGACTCCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG
    TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326712.1
    CDS182..5590
    Translation

    Target ORF information:

    RefSeq Version XM_021471037.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471037.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTG ATGATGCAGA TGAAGCCTGT ACACATCTGA ATAAAGTTCT GGGTGGAAAC
    CTGTTACTCT TGAGAGAGCT GAATCTGAGT AAACTTGATC TTGGACCATC AGGATTGGAG
    AAACTTGCTG CTGTACTGAA AGATAAACAC TGTAAACTCA ACACACTGAT TCTGAATAAC
    AGCAGTATGT CAGAAGAAGA TTGTCGTGTT TTGGCTGAAG CTTTTAATTC AAACCCATCA
    AATCTGACAG AGCTGAATTT GAGTGGGACT AAACTCACAA ACTCAGGAAT GAAGAGCTTC
    ACCACTCTGT TTCAAAACCA ACAGTGTAGA CTGGAAAAGC TGAAGTTCAG CAGCATGTGT
    ATTACAGCAG AAGGTTGTGC TGCTTTGGCT TCAGCATTTA ACTCAAATCC TTCACACCTG
    ATAGAGCTCA ATCTGAGTGA GAATAAACTA GGAGACTCAG GAGTAACAGA AATCTCCAGT
    CTACTGGCAA ACTCGCAGTG CATGCTGAAG ATACTCGGTC TTTCAGACTG CAGTATCAGT
    GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG
    CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG
    CAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGACGCC
    TGTCGGTTTG TGACTAAAAT TGTGGGTAAA AACCCATTAC TCCTGACAGA ACTGAATCTG
    AGTGATCGTA AACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA
    CACTGTAGAG TCAACACAAT AATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT
    GCTTTAACTT CAGCATTTAT TTTAAACCCA TCACACCTGA TAGAGCTCAA TCTGAGTGGG
    AATAAACTAG GAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TACACAATGC
    CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GAAGCTCACT GATTTACTGC ACAGTAGAAG CTGTAAATTC
    AGATTTCTGA AGAGTTCTGC TGCACAGGAA GCATGTGACT ATCTTACTAA AGTTCTGGGT
    ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT
    GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGAAGAA AATCAAGCTG
    AATAATTGTG AGCTGACAGA GAAAAGCTGT TCACTTCTAG CTGCAGTTCT CTCCTCCAAA
    ACCATCCTGA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAGAA
    ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAAA TACTCAGTCT TTCAGACTGC
    CGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT
    GACTTACTGG AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT
    GATGAAGGCT GTCACTTTGT GACTGAAATG GTGGGTAAAA ACCCATTACT CCTGAAAGAA
    CTGAATCTGA GTGATCGTGT ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTGCTG
    CAGGATAAAC ACTGTCAGAT CCACACACTC ATTCTGTGTT ATTGCAGTAT TACAGAGGAA
    CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC
    CTCAGTGGAA ACAATCTAGG AGACTCTGCA GTGAAAAAAC TCAGTGATTT ACTGATGAAC
    CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTGATTGCA GTATTACAGA GAAACAGTGT
    GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT
    GGGAATAGAC TAGGAGACTC TGGAGCTTAT GGACTTGGCC ATCTGCTGAG CAGTGTGGAT
    TGCAAGCTGA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGGGAAACA GTTGCACTTC
    CTGATTGCAG TTATGTGTAT GGACCCATCA CACCTGAGGG TACTGGACCT CAGTAAGAAT
    CAAATAAAAA GCAGAGGAAT GAACATGCTG TGTGATGTGC TGAATAAACC ACGCTGTAAA
    CTCAAGAGTT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACACTT AACTCAGTCT
    TTGGCTGAGA ACAAAGCACT GGAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG
    GGAGACTCCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG
    TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146941
    Clone ID Related Accession (Same CDS sequence) XM_021471038.1
    Accession Version XM_021471038.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NLR family CARD domain-containing protein 3 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGTCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GACGCCTGTC GGTTTGTGAC TAAAATTGTG GGTAAAAACC CATTACTCCT GACAGAACTG
    AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG
    GATAAACACT GTAGAGTCAA CACAATAATG CTGAATAATA ACAGGATTAC AGCAGAAGGT
    TGTGCTGCTT TAACTTCAGC ATTTATTTTA AACCCATCAC ACCTGATAGA GCTCAATCTG
    AGTGGGAATA AACTAGGAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATACA
    CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGAAG CTCACTGATT TACTGCACAG TAGAAGCTGT
    AAATTCAGAT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GTGACTATCT TACTAAAGTT
    CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT
    CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GAAGAAAATC
    AAGCTGAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC
    TCCAAAACCA TCCTGAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC
    AGAGAAATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAAATACT CAGTCTTTCA
    GACTGCCGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG
    CTCACTGACT TACTGGAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT
    GCTGCTGATG AAGGCTGTCA CTTTGTGACT GAAATGGTGG GTAAAAACCC ATTACTCCTG
    AAAGAACTGA ATCTGAGTGA TCGTGTACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT
    CTGCTGCAGG ATAAACACTG TCAGATCCAC ACACTCATTC TGTGTTATTG CAGTATTACA
    GAGGAACAGT GTGTGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA
    CTGAACCTCA GTGGAAACAA TCTAGGAGAC TCTGCAGTGA AAAAACTCAG TGATTTACTG
    ATGAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTG ATTGCAGTAT TACAGAGAAA
    CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC
    CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTTATGGAC TTGGCCATCT GCTGAGCAGT
    GTGGATTGCA AGCTGAATAA ACTACATCTG TGTGATTGCA GTATTACAGG GAAACAGTTG
    CACTTCCTGA TTGCAGTTAT GTGTATGGAC CCATCACACC TGAGGGTACT GGACCTCAGT
    AAGAATCAAA TAAAAAGCAG AGGAATGAAC ATGCTGTGTG ATGTGCTGAA TAAACCACGC
    TGTAAACTCA AGAGTTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACACTTAACT
    CAGTCTTTGG CTGAGAACAA AGCACTGGAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT
    CAAATGGGAG ACTCCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA
    CTGAGGTTAG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326713.1
    CDS182..5416
    Translation

    Target ORF information:

    RefSeq Version XM_021471038.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471038.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGTCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GACGCCTGTC GGTTTGTGAC TAAAATTGTG GGTAAAAACC CATTACTCCT GACAGAACTG
    AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG
    GATAAACACT GTAGAGTCAA CACAATAATG CTGAATAATA ACAGGATTAC AGCAGAAGGT
    TGTGCTGCTT TAACTTCAGC ATTTATTTTA AACCCATCAC ACCTGATAGA GCTCAATCTG
    AGTGGGAATA AACTAGGAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATACA
    CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGAAG CTCACTGATT TACTGCACAG TAGAAGCTGT
    AAATTCAGAT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GTGACTATCT TACTAAAGTT
    CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT
    CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GAAGAAAATC
    AAGCTGAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC
    TCCAAAACCA TCCTGAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC
    AGAGAAATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAAATACT CAGTCTTTCA
    GACTGCCGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG
    CTCACTGACT TACTGGAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT
    GCTGCTGATG AAGGCTGTCA CTTTGTGACT GAAATGGTGG GTAAAAACCC ATTACTCCTG
    AAAGAACTGA ATCTGAGTGA TCGTGTACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT
    CTGCTGCAGG ATAAACACTG TCAGATCCAC ACACTCATTC TGTGTTATTG CAGTATTACA
    GAGGAACAGT GTGTGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA
    CTGAACCTCA GTGGAAACAA TCTAGGAGAC TCTGCAGTGA AAAAACTCAG TGATTTACTG
    ATGAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTG ATTGCAGTAT TACAGAGAAA
    CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC
    CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTTATGGAC TTGGCCATCT GCTGAGCAGT
    GTGGATTGCA AGCTGAATAA ACTACATCTG TGTGATTGCA GTATTACAGG GAAACAGTTG
    CACTTCCTGA TTGCAGTTAT GTGTATGGAC CCATCACACC TGAGGGTACT GGACCTCAGT
    AAGAATCAAA TAAAAAGCAG AGGAATGAAC ATGCTGTGTG ATGTGCTGAA TAAACCACGC
    TGTAAACTCA AGAGTTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACACTTAACT
    CAGTCTTTGG CTGAGAACAA AGCACTGGAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT
    CAAATGGGAG ACTCCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA
    CTGAGGTTAG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146942
    Clone ID Related Accession (Same CDS sequence) XM_021471039.1
    Accession Version XM_021471039.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTGAGCTGA CAGAGAAAAG CTGTTCACTT
    CTAGCTGCAG TTCTCTCCTC CAAAACCATC CTGAAAGAGA TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAAATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAAATACTCA GTCTTTCAGA CTGCCGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGACTTA CTGGAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCACT TTGTGACTGA AATGGTGGGT
    AAAAACCCAT TACTCCTGAA AGAACTGAAT CTGAGTGATC GTGTACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT GCTGCAGGAT AAACACTGTC AGATCCACAC ACTCATTCTG
    TGTTATTGCA GTATTACAGA GGAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC
    CCATCACACC TGAGAGAACT GAACCTCAGT GGAAACAATC TAGGAGACTC TGCAGTGAAA
    AAACTCAGTG ATTTACTGAT GAACCAACAA TTCAAGCTGG AGAAACTACG TCTGAGTGAT
    TGCAGTATTA CAGAGAAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA
    CACCTGAGAG AACTGAACCT CAATGGGAAT AGACTAGGAG ACTCTGGAGC TTATGGACTT
    GGCCATCTGC TGAGCAGTGT GGATTGCAAG CTGAATAAAC TACATCTGTG TGATTGCAGT
    ATTACAGGGA AACAGTTGCA CTTCCTGATT GCAGTTATGT GTATGGACCC ATCACACCTG
    AGGGTACTGG ACCTCAGTAA GAATCAAATA AAAAGCAGAG GAATGAACAT GCTGTGTGAT
    GTGCTGAATA AACCACGCTG TAAACTCAAG AGTTTAAGAC TAAATGACTG TGGCATTACA
    GATGTTACAC ACTTAACTCA GTCTTTGGCT GAGAACAAAG CACTGGAGTT CTTAAAAGAG
    CTTGAACTGA GTAAAAATCA AATGGGAGAC TCCGAAAAGC AAAAACTCAG TGACCTGCTG
    CGAGACTCAA ACTGTAAACT GAGGTTAGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326714.1
    CDS182..5254
    Translation

    Target ORF information:

    RefSeq Version XM_021471039.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471039.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA
    CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG
    CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT
    GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT
    CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT
    CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT
    GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA
    CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC
    TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG
    GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG
    TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT
    CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG
    GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA
    CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG
    AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT
    GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT
    CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG
    AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC
    CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG
    CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC
    TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT
    TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG
    CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG
    AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG
    TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT
    CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG
    AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG
    CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT
    GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT
    GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT
    GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT
    AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTGAGCTGA CAGAGAAAAG CTGTTCACTT
    CTAGCTGCAG TTCTCTCCTC CAAAACCATC CTGAAAGAGA TGAACCTGAA CAACAGTCGT
    CTGCTGGACT CAGGAGTCAG AGAAATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG
    AAAATACTCA GTCTTTCAGA CTGCCGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA
    GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA
    CAATCAGGAG TGAAGGAGCT CACTGACTTA CTGGAGGATC CACACTGTAC ACTGAAGACA
    CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCACT TTGTGACTGA AATGGTGGGT
    AAAAACCCAT TACTCCTGAA AGAACTGAAT CTGAGTGATC GTGTACTAGG AGACACACGA
    GTGAATCAAC TCTCTGCTCT GCTGCAGGAT AAACACTGTC AGATCCACAC ACTCATTCTG
    TGTTATTGCA GTATTACAGA GGAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC
    CCATCACACC TGAGAGAACT GAACCTCAGT GGAAACAATC TAGGAGACTC TGCAGTGAAA
    AAACTCAGTG ATTTACTGAT GAACCAACAA TTCAAGCTGG AGAAACTACG TCTGAGTGAT
    TGCAGTATTA CAGAGAAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA
    CACCTGAGAG AACTGAACCT CAATGGGAAT AGACTAGGAG ACTCTGGAGC TTATGGACTT
    GGCCATCTGC TGAGCAGTGT GGATTGCAAG CTGAATAAAC TACATCTGTG TGATTGCAGT
    ATTACAGGGA AACAGTTGCA CTTCCTGATT GCAGTTATGT GTATGGACCC ATCACACCTG
    AGGGTACTGG ACCTCAGTAA GAATCAAATA AAAAGCAGAG GAATGAACAT GCTGTGTGAT
    GTGCTGAATA AACCACGCTG TAAACTCAAG AGTTTAAGAC TAAATGACTG TGGCATTACA
    GATGTTACAC ACTTAACTCA GTCTTTGGCT GAGAACAAAG CACTGGAGTT CTTAAAAGAG
    CTTGAACTGA GTAAAAATCA AATGGGAGAC TCCGAAAAGC AAAAACTCAG TGACCTGCTG
    CGAGACTCAA ACTGTAAACT GAGGTTAGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45489
    Clone ID Related Accession (Same CDS sequence) XM_009298420.2
    Accession Version XM_009298420.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein LOC568664 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298420.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCCAAA ACTTTCGATT AACTTACATT TTAGAAAACA TTGAAGGATC TCAGGTGTGT 
    TTGAGCTGTG ACTTAGTCTT TTTAAAGGTT TCTGGATTCT GTTTCTGCTG CACAAGACCG
    TCTTCTCGAC ATGTAAGATC AGGTTCGTAT GTATCCAGCT CTGTGTCTGT GAAGAGTGAT
    GATTCAAAAA AAGGAGGTCA GCCAAACTTC AGTAGAGAAA CACTGTCACC TACCACAAGT
    GTTCAAAATG AGTCTTCAGA CACAGACATC CAGACTCAGA AAAAACACAT GAGTTTTAAA
    GACAATCTTC AGGGGATCTT CCAGGATCTT GAGAACCAAA TATTAAAATT TCTGAAGAAT
    GAGCTAGAAA AGTTTAAGAA AATATTACAT GAAGAAAATA CAGAATACTT TGTAAAGGAC
    TTTGGTGAGA ACAGATGCAG AATAAAAGAA GCAGCTCTTG ATCTCACACT CTACTTCCTG
    AAAGAAATGA AGCAAGATGA AGCAGCAGAT ACTCTAGAAG ATGAGCTGTT TCTCATTCAT
    CAGCTGAAAT GGGATCTACA AAAGAAGTAT CAGCGTGTGT TTGAAGGAAT TGCACAGCAA
    GGAGACTCCA CACTCCTGAA GAACATCTAC ACAGATCTTT ATATCACTCA GGGTGGCAGT
    GAACAGGTCA ATACTGAACA TGAGGTCAGA CAGATTGAAG TTGTTTCTAG ACGACATGAA
    GCTCAAGAGA TACAGGTTGA ATGCAGACGT TTGTTTGAAG CGTCTGAACA AGACAAGCAG
    ATCAGAACTG TCCTGACAAA AGGAGTCGCT GGCATCGGGA AATCAGTCTC TGTGCAAAAG
    TTTGTTCTGG ATTGGGCCGA AGGAAAAGAA AATCAAGAGA TCAGCTTCAT ATTTCCTCTT
    CCATTCAGAG AGATGAACTT AAAGGAGAAA GAAACACTCA GTTTGATGGA GCTTATAACT
    CAGTTTTTCC CAGAGACGAA AGGACTGAAC CTTACAAGAA GGAATCAGTT CAAAGTCCTG
    TTCATCCTTG ATGGATTGGA TGAATGTCGT CTTCCTCTGA ACTTTGCTGG TAATGAGACG
    TGTCTAGATG TTGCATCACC AGCCTCTCTG GATGTTCTCC TGACGAACCT CATCAAGGGA
    AATCTGCTTC CTTCTGCTCT CATCTGGATC ACCAGCAGAC CAGCAGCTGC CAGTAAGATT
    CCTCCTGACT GTATCGACAG AGTGACAGAG ATACGAGGAT TCAATGATGA GCAAAAGGAG
    GAGTACTTCA GAAAAAGATT CACAGATGAG AATCAGGCCA AAGAAATCAT TGATCATGTT
    AAACAATCAA AGAGTCTCTT CATCATGTGC CACATCCCAG TCTTCTGTTG GATTTCAGCC
    ACTGTTCTCC AGAACATTTT AGAGGAGAAG AGAAATACCG TTGTGAAAAA CAATCAGGCT
    GATGACGCCT TCAAAACACT GCAGGAATCA AACACTGAAG ACACTCCCAA GACTCTGACA
    CAGATGTACA CACACTTTCT GCACTTTCAG ATCCAGCAGA GCAGACGAAA GTATGATGGA
    GAATACACAC CAGATGTGTC CTGGGATAAA GACGCCATCC TTTCACTGGG GAAACTGGCA
    TTTGATAATC TGCTAAACAG CAACCTGATC TTCTATGACA CCGACCTGGA AGCCTGTGGT
    ATTGATGTCT ATAAGGCCTC AGTTTACTCA GGCATGTGTA CCCAGATCTT TAAGGAGGAA
    ACAGGGATTG TTCTTGGCAC CATGTACTGC TTTGTTCACT TGAGCGTTCA AGAGTTTATT
    GCAGCCCTTT ATGCACATCT GTTACTAGAC ATCAACAAGA AAAGTGTGTT TGATCAAGAC
    TCTAAAAACA GAAATGAGTC CATGATTGAT TTGCTAAAGA CTGCAGTGGA CAAGACACTA
    GAGAGTGACA AAGGACACCT GGACCTTTTC CTTCGCTTCC TTCTCGGTCT GTCTCTCCAG
    TCCAATCAAC GGCTCTTACG AGGTCTGTTG ACACAGCAAG ACCACAATAA CCAGATCAAA
    GAGGAAATAG TTCAGTACAT CAAGCAGAAG TTTGAAGCGA ATCTTTCTCC AGAGAGATCC
    ATCAATCTGT TCTACTGTCT GAATGAACTG AACGACCAAA CTCTGGTGAA AGAGATTCAG
    ACTCACCTTA GCAAAGGAAG TCTCTCATCT GTTGATCTTT CACCTGCCCA GTGGTCTGCT
    CTGGCCTTTG TGTTGTTGAC ATCAGAGGAG AAGATGGAGG AGTTTGAGCT TCAGAAATTC
    AAGAAATCAG ACGAGTGTCT CATCAGACTA TCAGCAGTTA TCAAAACTAC CAGAAAAGCT
    CTGTTAAATG ATTGTAACTT AACAGACAGA AGCTGTTCAG CTCTGCCTGC AGTTCTCGGA
    GAAGAAAACA ATCTGAAAGA GCTGAACATG AGCAATAACA ATCTGCAGGA TTCAGGAGTG
    AAGCTGCTCT GCACTGGACT GAAAGATATT AAGTGCGAGT TAGAAATACT AAGACTTTCA
    GACTGCAGTA TTACAGAAGA AGGTTATAAA GCTCTGGCTT TAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG
    CTCACTGATT TACTGCAGGA TCCACACTGT ACACTGAAGA CACTAAGGTT TTTGAGTACT
    GCTGCAGAGG AAGCCTGTCA GTTTGTGCCT GGAGTTGTGG GTAAAAACCC GTTACTCCTT
    AAAGAACTGA ATCTGAGTGA TTGTAAACTA GGAGACAGGC GAGTGAACCA ACTCTCTGCT
    TTACTGCAGG ATAAACACTG TCAGATCAAC ACACTCTTTC TGTATAATTG CAGTATTACA
    GAGGAACAGT GTTTGATCCT GTCTTCAGCT CTGAAATCAA ACCCATCACA CTTGAGAAAA
    CTGAACCTCA GTGAGAATCA AATAAGAAAC ACAGGAGTGA ATCTCTTATG TGATGTACTG
    AAGGATTCAC ACTGTAAACT GAAGACACTG AGGCTTTCAG ACTGCAGTAT CAGTGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGATTT ACAGCAGGAT
    CCACACTGTA CACTGAAGAT ACTGAGGTTT TTGAGTCCTG CTGCTGATGA AGCCTGTCAG
    TTTTTGACTG GAGTTGTGGG TAAAAACCTG TTACTCTTGA AAGAGCTGAA TCTGAGTGGA
    CATGAACTAG GACTCTCAGA CAGCTTTCAA ATGCTTGTTC TTTTACTGCA TGATAAACAC
    TGTACGCTCA ACACACTGAT TCTTTCAGAT TGCAGTATCA CAGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGACATC AAACCCATCA TACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGGAACTC ACTGATTTAC TACTGGATGA ACGCTGTAGC
    TTAAAAATCA TCAGGTTTCT GAAAAGTCCT GCTGCACAAG AGTCATGTGA TTTTCTCACT
    GAAGTTCTGG ATGTAAATCC ATTATTACTG ACAGAACTGG ATCTGAGTGA AGATAAATTA
    GGAGATCTGG ATGGAGAGAA AATCTCTGCT TTTTTGATGG ACCCTCATAG CAAAGTGGAG
    AAAATCAAGC TGAATAATTG TAAGCTGCTT GAGAAAAGCT CATCAGTTTT AGCAACAGTT
    CTGTCTTCCA AAACCATCCT GAAAGAGCTG AACCTGAACA ACAGTCGTCT GCTGGACTCA
    GGAGTCAGAG AGATCTGTGA GGGACTGAAG AATCCTGTGT GTGAGCTGAA GATACTCAAG
    CTTTCAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA
    AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG
    AAGGAGCTCA CTGAGTTACT GCAGGATCCA CACTGTACAC TGAAGACACT GAGGTTTTTG
    AGTCCTGCTG CTGATGAAGG CTGTCAGTTT GTGACTGGAA TTGTGGGTAA AAACCCATTA
    CTTCTGAAAG AGCTAAATCT GAGTGATCGT GAACTAGGAG ACACACGAGT GAATCAACTC
    TCTGCTCTAC TGCAGGATAA ACACTGTAAA CTCAACACAC TCATTCTTAG TAAATGTGGT
    GTAACAGTGG AAAGCTGTTC AGCTCTGGCT ACAGTCCTGA GATCAGAGAC CAGTCTAAAA
    GAGCTTGACA TGAGCAACAA TAATCTGCAG GAATCAGGAG TGAAGAAACT CCAGAGCGGA
    TTAAAAAACA CAAACTGCAC ACTGGAAAAA CTCAGTCTTT TAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGA AGCTCACTGA GTTACTGAAA
    CCAGACTATA CACTGAAGAC ACTGAGGTTT TTGAGTGATG ATGCAGATGA AGCCTGTACA
    CATCTGAATA AAGTTCTGGG TGGAAACCTG TTACTCTTGA GAGAGCTGAA TCTGAGTAAA
    CTTGATCTTG GACCATCAGG ATTGGAGAAA CTTGCTGCTG TACTGAAAGA TAAACACTGT
    AAACTCAACA CACTGATTCT GAATAACAGC AGTATGTCAG AAGAAGATTG TCGTGTTTTG
    GCTGAAGCTT TTAATTCAAA CCCATCAAAT CTGACAGAGC TGAATTTGAG TGGGACTAAA
    CTCACAAACT CAGGAATGAA GAGCTTCACC ACTCTGTTTC AAAACCAACA GTGTAGACTG
    GAAAAGCTGA AGTTCAGCAG CATGTGTATT ACAGCAGAAG GTTGTGCTGC TTTGGCTTCA
    GCATTTAACT CAAATCCTTC ACACCTGATA GAGCTCAATC TGAGTGAGAA TAAACTAGGA
    GACTCAGGAG TAACAGAAAT CTCCAGTCTA CTGGCAAACT CGCAGTGCAT GCTGAAGATA
    CTCGGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG
    AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA
    GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTGAA GACACTGAGG
    TTTTTGAGTC CTGCTGCTGA TGACGCCTGT CGGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCATTACTCC TGACAGAACT GAATCTGAGT GATCGTAAAC TAGGAGACAC ACGAGTGAAT
    CAACTCTCTG CTCTACTGCA GGATAAACAC TGTAGAGTCA ACACAATAAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTAACTTCAG CATTTATTTT AAACCCATCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCGA TTAGAGAAAC TGAGTCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAT
    TTACTGCACA GTAGAAGCTG TAAATTCAGA TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA
    TGTGACTATC TTACTAAAGT TCTGGGTATA AGTCCATTAT TACTGACAGA ACTGGATCTG
    AGTGAAGATA AATTAGGAGA TCTGGATGGA GAGAAACTCT CTGCTCTTCT GATGGACTCT
    CATAGCAAAG TGAAGAAAAT CAAGCTGAAT AATTGTGAGC TGACAGAGAA AAGCTGTTCA
    CTTCTAGCTG CAGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT
    CGTCTGCTGG ACTCAGGAGT CAGAGAAATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG
    CTGAAAATAC TCAGTCTTTC AGACTGCCGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGGAGG ATCCACACTG TACACTGAAG
    ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAAGGCTGTC ACTTTGTGAC TGAAATGGTG
    GGTAAAAACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGTACT AGGAGACACA
    CGAGTGAATC AACTCTCTGC TCTGCTGCAG GATAAACACT GTCAGATCCA CACACTCATT
    CTGTGTTATT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGAAACA ATCTAGGAGA CTCTGCAGTG
    AAAAAACTCA GTGATTTACT GATGAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT
    GATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA
    TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTTATGGA
    CTTGGCCATC TGCTGAGCAG TGTGGATTGC AAGCTGAATA AACTACATCT GTGTGATTGC
    AGTATTACAG GGAAACAGTT GCACTTCCTG ATTGCAGTTA TGTGTATGGA CCCATCACAC
    CTGAGGGTAC TGGACCTCAG TAAGAATCAA ATAAAAAGCA GAGGAATGAA CATGCTGTGT
    GATGTGCTGA ATAAACCACG CTGTAAACTC AAGAGTTTAA GACTAAATGA CTGTGGCATT
    ACAGATGTTA CACACTTAAC TCAGTCTTTG GCTGAGAACA AAGCACTGGA GTTCTTAAAA
    GAGCTTGAAC TGAGTAAAAA TCAAATGGGA GACTCCGAAA AGCAAAAACT CAGTGACCTG
    CTGCGAGACT CAAACTGTAA ACTGAGGTTA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296695.1
    CDS25..7200
    Translation

    Target ORF information:

    RefSeq Version XM_009298420.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298420.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCCAAA ACTTTCGATT AACTTACATT TTAGAAAACA TTGAAGGATC TCAGGTGTGT 
    TTGAGCTGTG ACTTAGTCTT TTTAAAGGTT TCTGGATTCT GTTTCTGCTG CACAAGACCG
    TCTTCTCGAC ATGTAAGATC AGGTTCGTAT GTATCCAGCT CTGTGTCTGT GAAGAGTGAT
    GATTCAAAAA AAGGAGGTCA GCCAAACTTC AGTAGAGAAA CACTGTCACC TACCACAAGT
    GTTCAAAATG AGTCTTCAGA CACAGACATC CAGACTCAGA AAAAACACAT GAGTTTTAAA
    GACAATCTTC AGGGGATCTT CCAGGATCTT GAGAACCAAA TATTAAAATT TCTGAAGAAT
    GAGCTAGAAA AGTTTAAGAA AATATTACAT GAAGAAAATA CAGAATACTT TGTAAAGGAC
    TTTGGTGAGA ACAGATGCAG AATAAAAGAA GCAGCTCTTG ATCTCACACT CTACTTCCTG
    AAAGAAATGA AGCAAGATGA AGCAGCAGAT ACTCTAGAAG ATGAGCTGTT TCTCATTCAT
    CAGCTGAAAT GGGATCTACA AAAGAAGTAT CAGCGTGTGT TTGAAGGAAT TGCACAGCAA
    GGAGACTCCA CACTCCTGAA GAACATCTAC ACAGATCTTT ATATCACTCA GGGTGGCAGT
    GAACAGGTCA ATACTGAACA TGAGGTCAGA CAGATTGAAG TTGTTTCTAG ACGACATGAA
    GCTCAAGAGA TACAGGTTGA ATGCAGACGT TTGTTTGAAG CGTCTGAACA AGACAAGCAG
    ATCAGAACTG TCCTGACAAA AGGAGTCGCT GGCATCGGGA AATCAGTCTC TGTGCAAAAG
    TTTGTTCTGG ATTGGGCCGA AGGAAAAGAA AATCAAGAGA TCAGCTTCAT ATTTCCTCTT
    CCATTCAGAG AGATGAACTT AAAGGAGAAA GAAACACTCA GTTTGATGGA GCTTATAACT
    CAGTTTTTCC CAGAGACGAA AGGACTGAAC CTTACAAGAA GGAATCAGTT CAAAGTCCTG
    TTCATCCTTG ATGGATTGGA TGAATGTCGT CTTCCTCTGA ACTTTGCTGG TAATGAGACG
    TGTCTAGATG TTGCATCACC AGCCTCTCTG GATGTTCTCC TGACGAACCT CATCAAGGGA
    AATCTGCTTC CTTCTGCTCT CATCTGGATC ACCAGCAGAC CAGCAGCTGC CAGTAAGATT
    CCTCCTGACT GTATCGACAG AGTGACAGAG ATACGAGGAT TCAATGATGA GCAAAAGGAG
    GAGTACTTCA GAAAAAGATT CACAGATGAG AATCAGGCCA AAGAAATCAT TGATCATGTT
    AAACAATCAA AGAGTCTCTT CATCATGTGC CACATCCCAG TCTTCTGTTG GATTTCAGCC
    ACTGTTCTCC AGAACATTTT AGAGGAGAAG AGAAATACCG TTGTGAAAAA CAATCAGGCT
    GATGACGCCT TCAAAACACT GCAGGAATCA AACACTGAAG ACACTCCCAA GACTCTGACA
    CAGATGTACA CACACTTTCT GCACTTTCAG ATCCAGCAGA GCAGACGAAA GTATGATGGA
    GAATACACAC CAGATGTGTC CTGGGATAAA GACGCCATCC TTTCACTGGG GAAACTGGCA
    TTTGATAATC TGCTAAACAG CAACCTGATC TTCTATGACA CCGACCTGGA AGCCTGTGGT
    ATTGATGTCT ATAAGGCCTC AGTTTACTCA GGCATGTGTA CCCAGATCTT TAAGGAGGAA
    ACAGGGATTG TTCTTGGCAC CATGTACTGC TTTGTTCACT TGAGCGTTCA AGAGTTTATT
    GCAGCCCTTT ATGCACATCT GTTACTAGAC ATCAACAAGA AAAGTGTGTT TGATCAAGAC
    TCTAAAAACA GAAATGAGTC CATGATTGAT TTGCTAAAGA CTGCAGTGGA CAAGACACTA
    GAGAGTGACA AAGGACACCT GGACCTTTTC CTTCGCTTCC TTCTCGGTCT GTCTCTCCAG
    TCCAATCAAC GGCTCTTACG AGGTCTGTTG ACACAGCAAG ACCACAATAA CCAGATCAAA
    GAGGAAATAG TTCAGTACAT CAAGCAGAAG TTTGAAGCGA ATCTTTCTCC AGAGAGATCC
    ATCAATCTGT TCTACTGTCT GAATGAACTG AACGACCAAA CTCTGGTGAA AGAGATTCAG
    ACTCACCTTA GCAAAGGAAG TCTCTCATCT GTTGATCTTT CACCTGCCCA GTGGTCTGCT
    CTGGCCTTTG TGTTGTTGAC ATCAGAGGAG AAGATGGAGG AGTTTGAGCT TCAGAAATTC
    AAGAAATCAG ACGAGTGTCT CATCAGACTA TCAGCAGTTA TCAAAACTAC CAGAAAAGCT
    CTGTTAAATG ATTGTAACTT AACAGACAGA AGCTGTTCAG CTCTGCCTGC AGTTCTCGGA
    GAAGAAAACA ATCTGAAAGA GCTGAACATG AGCAATAACA ATCTGCAGGA TTCAGGAGTG
    AAGCTGCTCT GCACTGGACT GAAAGATATT AAGTGCGAGT TAGAAATACT AAGACTTTCA
    GACTGCAGTA TTACAGAAGA AGGTTATAAA GCTCTGGCTT TAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG
    CTCACTGATT TACTGCAGGA TCCACACTGT ACACTGAAGA CACTAAGGTT TTTGAGTACT
    GCTGCAGAGG AAGCCTGTCA GTTTGTGCCT GGAGTTGTGG GTAAAAACCC GTTACTCCTT
    AAAGAACTGA ATCTGAGTGA TTGTAAACTA GGAGACAGGC GAGTGAACCA ACTCTCTGCT
    TTACTGCAGG ATAAACACTG TCAGATCAAC ACACTCTTTC TGTATAATTG CAGTATTACA
    GAGGAACAGT GTTTGATCCT GTCTTCAGCT CTGAAATCAA ACCCATCACA CTTGAGAAAA
    CTGAACCTCA GTGAGAATCA AATAAGAAAC ACAGGAGTGA ATCTCTTATG TGATGTACTG
    AAGGATTCAC ACTGTAAACT GAAGACACTG AGGCTTTCAG ACTGCAGTAT CAGTGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGATTT ACAGCAGGAT
    CCACACTGTA CACTGAAGAT ACTGAGGTTT TTGAGTCCTG CTGCTGATGA AGCCTGTCAG
    TTTTTGACTG GAGTTGTGGG TAAAAACCTG TTACTCTTGA AAGAGCTGAA TCTGAGTGGA
    CATGAACTAG GACTCTCAGA CAGCTTTCAA ATGCTTGTTC TTTTACTGCA TGATAAACAC
    TGTACGCTCA ACACACTGAT TCTTTCAGAT TGCAGTATCA CAGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGACATC AAACCCATCA TACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGGAACTC ACTGATTTAC TACTGGATGA ACGCTGTAGC
    TTAAAAATCA TCAGGTTTCT GAAAAGTCCT GCTGCACAAG AGTCATGTGA TTTTCTCACT
    GAAGTTCTGG ATGTAAATCC ATTATTACTG ACAGAACTGG ATCTGAGTGA AGATAAATTA
    GGAGATCTGG ATGGAGAGAA AATCTCTGCT TTTTTGATGG ACCCTCATAG CAAAGTGGAG
    AAAATCAAGC TGAATAATTG TAAGCTGCTT GAGAAAAGCT CATCAGTTTT AGCAACAGTT
    CTGTCTTCCA AAACCATCCT GAAAGAGCTG AACCTGAACA ACAGTCGTCT GCTGGACTCA
    GGAGTCAGAG AGATCTGTGA GGGACTGAAG AATCCTGTGT GTGAGCTGAA GATACTCAAG
    CTTTCAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA
    AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG
    AAGGAGCTCA CTGAGTTACT GCAGGATCCA CACTGTACAC TGAAGACACT GAGGTTTTTG
    AGTCCTGCTG CTGATGAAGG CTGTCAGTTT GTGACTGGAA TTGTGGGTAA AAACCCATTA
    CTTCTGAAAG AGCTAAATCT GAGTGATCGT GAACTAGGAG ACACACGAGT GAATCAACTC
    TCTGCTCTAC TGCAGGATAA ACACTGTAAA CTCAACACAC TCATTCTTAG TAAATGTGGT
    GTAACAGTGG AAAGCTGTTC AGCTCTGGCT ACAGTCCTGA GATCAGAGAC CAGTCTAAAA
    GAGCTTGACA TGAGCAACAA TAATCTGCAG GAATCAGGAG TGAAGAAACT CCAGAGCGGA
    TTAAAAAACA CAAACTGCAC ACTGGAAAAA CTCAGTCTTT TAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGA AGCTCACTGA GTTACTGAAA
    CCAGACTATA CACTGAAGAC ACTGAGGTTT TTGAGTGATG ATGCAGATGA AGCCTGTACA
    CATCTGAATA AAGTTCTGGG TGGAAACCTG TTACTCTTGA GAGAGCTGAA TCTGAGTAAA
    CTTGATCTTG GACCATCAGG ATTGGAGAAA CTTGCTGCTG TACTGAAAGA TAAACACTGT
    AAACTCAACA CACTGATTCT GAATAACAGC AGTATGTCAG AAGAAGATTG TCGTGTTTTG
    GCTGAAGCTT TTAATTCAAA CCCATCAAAT CTGACAGAGC TGAATTTGAG TGGGACTAAA
    CTCACAAACT CAGGAATGAA GAGCTTCACC ACTCTGTTTC AAAACCAACA GTGTAGACTG
    GAAAAGCTGA AGTTCAGCAG CATGTGTATT ACAGCAGAAG GTTGTGCTGC TTTGGCTTCA
    GCATTTAACT CAAATCCTTC ACACCTGATA GAGCTCAATC TGAGTGAGAA TAAACTAGGA
    GACTCAGGAG TAACAGAAAT CTCCAGTCTA CTGGCAAACT CGCAGTGCAT GCTGAAGATA
    CTCGGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG
    AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA
    GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTGAA GACACTGAGG
    TTTTTGAGTC CTGCTGCTGA TGACGCCTGT CGGTTTGTGA CTAAAATTGT GGGTAAAAAC
    CCATTACTCC TGACAGAACT GAATCTGAGT GATCGTAAAC TAGGAGACAC ACGAGTGAAT
    CAACTCTCTG CTCTACTGCA GGATAAACAC TGTAGAGTCA ACACAATAAT GCTGAATAAT
    AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTAACTTCAG CATTTATTTT AAACCCATCA
    CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC
    TGTCCTCTGC TGGAAAATAC ACAATGCCGA TTAGAGAAAC TGAGTCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAT
    TTACTGCACA GTAGAAGCTG TAAATTCAGA TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA
    TGTGACTATC TTACTAAAGT TCTGGGTATA AGTCCATTAT TACTGACAGA ACTGGATCTG
    AGTGAAGATA AATTAGGAGA TCTGGATGGA GAGAAACTCT CTGCTCTTCT GATGGACTCT
    CATAGCAAAG TGAAGAAAAT CAAGCTGAAT AATTGTGAGC TGACAGAGAA AAGCTGTTCA
    CTTCTAGCTG CAGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT
    CGTCTGCTGG ACTCAGGAGT CAGAGAAATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG
    CTGAAAATAC TCAGTCTTTC AGACTGCCGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGGAGG ATCCACACTG TACACTGAAG
    ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAAGGCTGTC ACTTTGTGAC TGAAATGGTG
    GGTAAAAACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGTACT AGGAGACACA
    CGAGTGAATC AACTCTCTGC TCTGCTGCAG GATAAACACT GTCAGATCCA CACACTCATT
    CTGTGTTATT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGAAACA ATCTAGGAGA CTCTGCAGTG
    AAAAAACTCA GTGATTTACT GATGAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT
    GATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA
    TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTTATGGA
    CTTGGCCATC TGCTGAGCAG TGTGGATTGC AAGCTGAATA AACTACATCT GTGTGATTGC
    AGTATTACAG GGAAACAGTT GCACTTCCTG ATTGCAGTTA TGTGTATGGA CCCATCACAC
    CTGAGGGTAC TGGACCTCAG TAAGAATCAA ATAAAAAGCA GAGGAATGAA CATGCTGTGT
    GATGTGCTGA ATAAACCACG CTGTAAACTC AAGAGTTTAA GACTAAATGA CTGTGGCATT
    ACAGATGTTA CACACTTAAC TCAGTCTTTG GCTGAGAACA AAGCACTGGA GTTCTTAAAA
    GAGCTTGAAC TGAGTAAAAA TCAAATGGGA GACTCCGAAA AGCAAAAACT CAGTGACCTG
    CTGCGAGACT CAAACTGTAA ACTGAGGTTA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45490
    Clone ID Related Accession (Same CDS sequence) XM_009298421.2
    Accession Version XM_009298421.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein LOC568664 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGTCAGAGG AGCGAGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAG ATCAGGTTCG 
    TATGTATCCA GCTCTGTGTC TGTGAAGAGT GATGATTCAA AAAAAGGAGG TCAGCCAAAC
    TTCAGTAGAG AAACACTGTC ACCTACCACA AGTGTTCAAA ATGAGTCTTC AGACACAGAC
    ATCCAGACTC AGAAAAAACA CATGAGTTTT AAAGACAATC TTCAGGGGAT CTTCCAGGAT
    CTTGAGAACC AAATATTAAA ATTTCTGAAG AATGAGCTAG AAAAGTTTAA GAAAATATTA
    CATGAAGAAA ATACAGAATA CTTTGTAAAG GACTTTGGTG AGAACAGATG CAGAATAAAA
    GAAGCAGCTC TTGATCTCAC ACTCTACTTC CTGAAAGAAA TGAAGCAAGA TGAAGCAGCA
    GATACTCTAG AAGATGAGCT GTTTCTCATT CATCAGCTGA AATGGGATCT ACAAAAGAAG
    TATCAGCGTG TGTTTGAAGG AATTGCACAG CAAGGAGACT CCACACTCCT GAAGAACATC
    TACACAGATC TTTATATCAC TCAGGGTGGC AGTGAACAGG TCAATACTGA ACATGAGGTC
    AGACAGATTG AAGTTGTTTC TAGACGACAT GAAGCTCAAG AGATACAGGT TGAATGCAGA
    CGTTTGTTTG AAGCGTCTGA ACAAGACAAG CAGATCAGAA CTGTCCTGAC AAAAGGAGTC
    GCTGGCATCG GGAAATCAGT CTCTGTGCAA AAGTTTGTTC TGGATTGGGC CGAAGGAAAA
    GAAAATCAAG AGATCAGCTT CATATTTCCT CTTCCATTCA GAGAGATGAA CTTAAAGGAG
    AAAGAAACAC TCAGTTTGAT GGAGCTTATA ACTCAGTTTT TCCCAGAGAC GAAAGGACTG
    AACCTTACAA GAAGGAATCA GTTCAAAGTC CTGTTCATCC TTGATGGATT GGATGAATGT
    CGTCTTCCTC TGAACTTTGC TGGTAATGAG ACGTGTCTAG ATGTTGCATC ACCAGCCTCT
    CTGGATGTTC TCCTGACGAA CCTCATCAAG GGAAATCTGC TTCCTTCTGC TCTCATCTGG
    ATCACCAGCA GACCAGCAGC TGCCAGTAAG ATTCCTCCTG ACTGTATCGA CAGAGTGACA
    GAGATACGAG GATTCAATGA TGAGCAAAAG GAGGAGTACT TCAGAAAAAG ATTCACAGAT
    GAGAATCAGG CCAAAGAAAT CATTGATCAT GTTAAACAAT CAAAGAGTCT CTTCATCATG
    TGCCACATCC CAGTCTTCTG TTGGATTTCA GCCACTGTTC TCCAGAACAT TTTAGAGGAG
    AAGAGAAATA CCGTTGTGAA AAACAATCAG GCTGATGACG CCTTCAAAAC ACTGCAGGAA
    TCAAACACTG AAGACACTCC CAAGACTCTG ACACAGATGT ACACACACTT TCTGCACTTT
    CAGATCCAGC AGAGCAGACG AAAGTATGAT GGAGAATACA CACCAGATGT GTCCTGGGAT
    AAAGACGCCA TCCTTTCACT GGGGAAACTG GCATTTGATA ATCTGCTAAA CAGCAACCTG
    ATCTTCTATG ACACCGACCT GGAAGCCTGT GGTATTGATG TCTATAAGGC CTCAGTTTAC
    TCAGGCATGT GTACCCAGAT CTTTAAGGAG GAAACAGGGA TTGTTCTTGG CACCATGTAC
    TGCTTTGTTC ACTTGAGCGT TCAAGAGTTT ATTGCAGCCC TTTATGCACA TCTGTTACTA
    GACATCAACA AGAAAAGTGT GTTTGATCAA GACTCTAAAA ACAGAAATGA GTCCATGATT
    GATTTGCTAA AGACTGCAGT GGACAAGACA CTAGAGAGTG ACAAAGGACA CCTGGACCTT
    TTCCTTCGCT TCCTTCTCGG TCTGTCTCTC CAGTCCAATC AACGGCTCTT ACGAGGTCTG
    TTGACACAGC AAGACCACAA TAACCAGATC AAAGAGGAAA TAGTTCAGTA CATCAAGCAG
    AAGTTTGAAG CGAATCTTTC TCCAGAGAGA TCCATCAATC TGTTCTACTG TCTGAATGAA
    CTGAACGACC AAACTCTGGT GAAAGAGATT CAGACTCACC TTAGCAAAGG AAGTCTCTCA
    TCTGTTGATC TTTCACCTGC CCAGTGGTCT GCTCTGGCCT TTGTGTTGTT GACATCAGAG
    GAGAAGATGG AGGAGTTTGA GCTTCAGAAA TTCAAGAAAT CAGACGAGTG TCTCATCAGA
    CTATCAGCAG TTATCAAAAC TACCAGAAAA GCTCTGTTAA ATGATTGTAA CTTAACAGAC
    AGAAGCTGTT CAGCTCTGCC TGCAGTTCTC GGAGAAGAAA ACAATCTGAA AGAGCTGAAC
    ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGG ACTGAAAGAT
    ATTAAGTGCG AGTTAGAAAT ACTAAGACTT TCAGACTGCA GTATTACAGA AGAAGGTTAT
    AAAGCTCTGG CTTTAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA
    GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ATTTACTGCA GGATCCACAC
    TGTACACTGA AGACACTAAG GTTTTTGAGT ACTGCTGCAG AGGAAGCCTG TCAGTTTGTG
    CCTGGAGTTG TGGGTAAAAA CCCGTTACTC CTTAAAGAAC TGAATCTGAG TGATTGTAAA
    CTAGGAGACA GGCGAGTGAA CCAACTCTCT GCTTTACTGC AGGATAAACA CTGTCAGATC
    AACACACTCT TTCTGTATAA TTGCAGTATT ACAGAGGAAC AGTGTTTGAT CCTGTCTTCA
    GCTCTGAAAT CAAACCCATC ACACTTGAGA AAACTGAACC TCAGTGAGAA TCAAATAAGA
    AACACAGGAG TGAATCTCTT ATGTGATGTA CTGAAGGATT CACACTGTAA ACTGAAGACA
    CTGAGGCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG
    AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA
    GGAGTGAAGG AGCTCACTGA TTTACAGCAG GATCCACACT GTACACTGAA GATACTGAGG
    TTTTTGAGTC CTGCTGCTGA TGAAGCCTGT CAGTTTTTGA CTGGAGTTGT GGGTAAAAAC
    CTGTTACTCT TGAAAGAGCT GAATCTGAGT GGACATGAAC TAGGACTCTC AGACAGCTTT
    CAAATGCTTG TTCTTTTACT GCATGATAAA CACTGTACGC TCAACACACT GATTCTTTCA
    GATTGCAGTA TCACAGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAC ATCAAACCCA
    TCATACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAA
    CTCACTGATT TACTACTGGA TGAACGCTGT AGCTTAAAAA TCATCAGGTT TCTGAAAAGT
    CCTGCTGCAC AAGAGTCATG TGATTTTCTC ACTGAAGTTC TGGATGTAAA TCCATTATTA
    CTGACAGAAC TGGATCTGAG TGAAGATAAA TTAGGAGATC TGGATGGAGA GAAAATCTCT
    GCTTTTTTGA TGGACCCTCA TAGCAAAGTG GAGAAAATCA AGCTGAATAA TTGTAAGCTG
    CTTGAGAAAA GCTCATCAGT TTTAGCAACA GTTCTGTCTT CCAAAACCAT CCTGAAAGAG
    CTGAACCTGA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA GAGAGATCTG TGAGGGACTG
    AAGAATCCTG TGTGTGAGCT GAAGATACTC AAGCTTTCAG ACTGCAGTAT CAGTGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGAGTT ACTGCAGGAT
    CCACACTGTA CACTGAAGAC ACTGAGGTTT TTGAGTCCTG CTGCTGATGA AGGCTGTCAG
    TTTGTGACTG GAATTGTGGG TAAAAACCCA TTACTTCTGA AAGAGCTAAA TCTGAGTGAT
    CGTGAACTAG GAGACACACG AGTGAATCAA CTCTCTGCTC TACTGCAGGA TAAACACTGT
    AAACTCAACA CACTCATTCT TAGTAAATGT GGTGTAACAG TGGAAAGCTG TTCAGCTCTG
    GCTACAGTCC TGAGATCAGA GACCAGTCTA AAAGAGCTTG ACATGAGCAA CAATAATCTG
    CAGGAATCAG GAGTGAAGAA ACTCCAGAGC GGATTAAAAA ACACAAACTG CACACTGGAA
    AAACTCAGTC TTTTAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGAAGCTCAC TGAGTTACTG AAACCAGACT ATACACTGAA GACACTGAGG
    TTTTTGAGTG ATGATGCAGA TGAAGCCTGT ACACATCTGA ATAAAGTTCT GGGTGGAAAC
    CTGTTACTCT TGAGAGAGCT GAATCTGAGT AAACTTGATC TTGGACCATC AGGATTGGAG
    AAACTTGCTG CTGTACTGAA AGATAAACAC TGTAAACTCA ACACACTGAT TCTGAATAAC
    AGCAGTATGT CAGAAGAAGA TTGTCGTGTT TTGGCTGAAG CTTTTAATTC AAACCCATCA
    AATCTGACAG AGCTGAATTT GAGTGGGACT AAACTCACAA ACTCAGGAAT GAAGAGCTTC
    ACCACTCTGT TTCAAAACCA ACAGTGTAGA CTGGAAAAGC TGAAGTTCAG CAGCATGTGT
    ATTACAGCAG AAGGTTGTGC TGCTTTGGCT TCAGCATTTA ACTCAAATCC TTCACACCTG
    ATAGAGCTCA ATCTGAGTGA GAATAAACTA GGAGACTCAG GAGTAACAGA AATCTCCAGT
    CTACTGGCAA ACTCGCAGTG CATGCTGAAG ATACTCGGTC TTTCAGACTG CAGTATCAGT
    GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG
    CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG
    CAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGACGCC
    TGTCGGTTTG TGACTAAAAT TGTGGGTAAA AACCCATTAC TCCTGACAGA ACTGAATCTG
    AGTGATCGTA AACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA
    CACTGTAGAG TCAACACAAT AATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT
    GCTTTAACTT CAGCATTTAT TTTAAACCCA TCACACCTGA TAGAGCTCAA TCTGAGTGGG
    AATAAACTAG GAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TACACAATGC
    CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GAAGCTCACT GATTTACTGC ACAGTAGAAG CTGTAAATTC
    AGATTTCTGA AGAGTTCTGC TGCACAGGAA GCATGTGACT ATCTTACTAA AGTTCTGGGT
    ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT
    GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGAAGAA AATCAAGCTG
    AATAATTGTG AGCTGACAGA GAAAAGCTGT TCACTTCTAG CTGCAGTTCT CTCCTCCAAA
    ACCATCCTGA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAGAA
    ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAAA TACTCAGTCT TTCAGACTGC
    CGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT
    GACTTACTGG AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT
    GATGAAGGCT GTCACTTTGT GACTGAAATG GTGGGTAAAA ACCCATTACT CCTGAAAGAA
    CTGAATCTGA GTGATCGTGT ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTGCTG
    CAGGATAAAC ACTGTCAGAT CCACACACTC ATTCTGTGTT ATTGCAGTAT TACAGAGGAA
    CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC
    CTCAGTGGAA ACAATCTAGG AGACTCTGCA GTGAAAAAAC TCAGTGATTT ACTGATGAAC
    CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTGATTGCA GTATTACAGA GAAACAGTGT
    GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT
    GGGAATAGAC TAGGAGACTC TGGAGCTTAT GGACTTGGCC ATCTGCTGAG CAGTGTGGAT
    TGCAAGCTGA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGGGAAACA GTTGCACTTC
    CTGATTGCAG TTATGTGTAT GGACCCATCA CACCTGAGGG TACTGGACCT CAGTAAGAAT
    CAAATAAAAA GCAGAGGAAT GAACATGCTG TGTGATGTGC TGAATAAACC ACGCTGTAAA
    CTCAAGAGTT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACACTT AACTCAGTCT
    TTGGCTGAGA ACAAAGCACT GGAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG
    GGAGACTCCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG
    TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296696.1
    CDS117..7205
    Translation

    Target ORF information:

    RefSeq Version XM_009298421.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298421.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGTCAGAGG AGCGAGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAG ATCAGGTTCG 
    TATGTATCCA GCTCTGTGTC TGTGAAGAGT GATGATTCAA AAAAAGGAGG TCAGCCAAAC
    TTCAGTAGAG AAACACTGTC ACCTACCACA AGTGTTCAAA ATGAGTCTTC AGACACAGAC
    ATCCAGACTC AGAAAAAACA CATGAGTTTT AAAGACAATC TTCAGGGGAT CTTCCAGGAT
    CTTGAGAACC AAATATTAAA ATTTCTGAAG AATGAGCTAG AAAAGTTTAA GAAAATATTA
    CATGAAGAAA ATACAGAATA CTTTGTAAAG GACTTTGGTG AGAACAGATG CAGAATAAAA
    GAAGCAGCTC TTGATCTCAC ACTCTACTTC CTGAAAGAAA TGAAGCAAGA TGAAGCAGCA
    GATACTCTAG AAGATGAGCT GTTTCTCATT CATCAGCTGA AATGGGATCT ACAAAAGAAG
    TATCAGCGTG TGTTTGAAGG AATTGCACAG CAAGGAGACT CCACACTCCT GAAGAACATC
    TACACAGATC TTTATATCAC TCAGGGTGGC AGTGAACAGG TCAATACTGA ACATGAGGTC
    AGACAGATTG AAGTTGTTTC TAGACGACAT GAAGCTCAAG AGATACAGGT TGAATGCAGA
    CGTTTGTTTG AAGCGTCTGA ACAAGACAAG CAGATCAGAA CTGTCCTGAC AAAAGGAGTC
    GCTGGCATCG GGAAATCAGT CTCTGTGCAA AAGTTTGTTC TGGATTGGGC CGAAGGAAAA
    GAAAATCAAG AGATCAGCTT CATATTTCCT CTTCCATTCA GAGAGATGAA CTTAAAGGAG
    AAAGAAACAC TCAGTTTGAT GGAGCTTATA ACTCAGTTTT TCCCAGAGAC GAAAGGACTG
    AACCTTACAA GAAGGAATCA GTTCAAAGTC CTGTTCATCC TTGATGGATT GGATGAATGT
    CGTCTTCCTC TGAACTTTGC TGGTAATGAG ACGTGTCTAG ATGTTGCATC ACCAGCCTCT
    CTGGATGTTC TCCTGACGAA CCTCATCAAG GGAAATCTGC TTCCTTCTGC TCTCATCTGG
    ATCACCAGCA GACCAGCAGC TGCCAGTAAG ATTCCTCCTG ACTGTATCGA CAGAGTGACA
    GAGATACGAG GATTCAATGA TGAGCAAAAG GAGGAGTACT TCAGAAAAAG ATTCACAGAT
    GAGAATCAGG CCAAAGAAAT CATTGATCAT GTTAAACAAT CAAAGAGTCT CTTCATCATG
    TGCCACATCC CAGTCTTCTG TTGGATTTCA GCCACTGTTC TCCAGAACAT TTTAGAGGAG
    AAGAGAAATA CCGTTGTGAA AAACAATCAG GCTGATGACG CCTTCAAAAC ACTGCAGGAA
    TCAAACACTG AAGACACTCC CAAGACTCTG ACACAGATGT ACACACACTT TCTGCACTTT
    CAGATCCAGC AGAGCAGACG AAAGTATGAT GGAGAATACA CACCAGATGT GTCCTGGGAT
    AAAGACGCCA TCCTTTCACT GGGGAAACTG GCATTTGATA ATCTGCTAAA CAGCAACCTG
    ATCTTCTATG ACACCGACCT GGAAGCCTGT GGTATTGATG TCTATAAGGC CTCAGTTTAC
    TCAGGCATGT GTACCCAGAT CTTTAAGGAG GAAACAGGGA TTGTTCTTGG CACCATGTAC
    TGCTTTGTTC ACTTGAGCGT TCAAGAGTTT ATTGCAGCCC TTTATGCACA TCTGTTACTA
    GACATCAACA AGAAAAGTGT GTTTGATCAA GACTCTAAAA ACAGAAATGA GTCCATGATT
    GATTTGCTAA AGACTGCAGT GGACAAGACA CTAGAGAGTG ACAAAGGACA CCTGGACCTT
    TTCCTTCGCT TCCTTCTCGG TCTGTCTCTC CAGTCCAATC AACGGCTCTT ACGAGGTCTG
    TTGACACAGC AAGACCACAA TAACCAGATC AAAGAGGAAA TAGTTCAGTA CATCAAGCAG
    AAGTTTGAAG CGAATCTTTC TCCAGAGAGA TCCATCAATC TGTTCTACTG TCTGAATGAA
    CTGAACGACC AAACTCTGGT GAAAGAGATT CAGACTCACC TTAGCAAAGG AAGTCTCTCA
    TCTGTTGATC TTTCACCTGC CCAGTGGTCT GCTCTGGCCT TTGTGTTGTT GACATCAGAG
    GAGAAGATGG AGGAGTTTGA GCTTCAGAAA TTCAAGAAAT CAGACGAGTG TCTCATCAGA
    CTATCAGCAG TTATCAAAAC TACCAGAAAA GCTCTGTTAA ATGATTGTAA CTTAACAGAC
    AGAAGCTGTT CAGCTCTGCC TGCAGTTCTC GGAGAAGAAA ACAATCTGAA AGAGCTGAAC
    ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGG ACTGAAAGAT
    ATTAAGTGCG AGTTAGAAAT ACTAAGACTT TCAGACTGCA GTATTACAGA AGAAGGTTAT
    AAAGCTCTGG CTTTAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA
    GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ATTTACTGCA GGATCCACAC
    TGTACACTGA AGACACTAAG GTTTTTGAGT ACTGCTGCAG AGGAAGCCTG TCAGTTTGTG
    CCTGGAGTTG TGGGTAAAAA CCCGTTACTC CTTAAAGAAC TGAATCTGAG TGATTGTAAA
    CTAGGAGACA GGCGAGTGAA CCAACTCTCT GCTTTACTGC AGGATAAACA CTGTCAGATC
    AACACACTCT TTCTGTATAA TTGCAGTATT ACAGAGGAAC AGTGTTTGAT CCTGTCTTCA
    GCTCTGAAAT CAAACCCATC ACACTTGAGA AAACTGAACC TCAGTGAGAA TCAAATAAGA
    AACACAGGAG TGAATCTCTT ATGTGATGTA CTGAAGGATT CACACTGTAA ACTGAAGACA
    CTGAGGCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG
    AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA
    GGAGTGAAGG AGCTCACTGA TTTACAGCAG GATCCACACT GTACACTGAA GATACTGAGG
    TTTTTGAGTC CTGCTGCTGA TGAAGCCTGT CAGTTTTTGA CTGGAGTTGT GGGTAAAAAC
    CTGTTACTCT TGAAAGAGCT GAATCTGAGT GGACATGAAC TAGGACTCTC AGACAGCTTT
    CAAATGCTTG TTCTTTTACT GCATGATAAA CACTGTACGC TCAACACACT GATTCTTTCA
    GATTGCAGTA TCACAGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAC ATCAAACCCA
    TCATACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAA
    CTCACTGATT TACTACTGGA TGAACGCTGT AGCTTAAAAA TCATCAGGTT TCTGAAAAGT
    CCTGCTGCAC AAGAGTCATG TGATTTTCTC ACTGAAGTTC TGGATGTAAA TCCATTATTA
    CTGACAGAAC TGGATCTGAG TGAAGATAAA TTAGGAGATC TGGATGGAGA GAAAATCTCT
    GCTTTTTTGA TGGACCCTCA TAGCAAAGTG GAGAAAATCA AGCTGAATAA TTGTAAGCTG
    CTTGAGAAAA GCTCATCAGT TTTAGCAACA GTTCTGTCTT CCAAAACCAT CCTGAAAGAG
    CTGAACCTGA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA GAGAGATCTG TGAGGGACTG
    AAGAATCCTG TGTGTGAGCT GAAGATACTC AAGCTTTCAG ACTGCAGTAT CAGTGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGAGTT ACTGCAGGAT
    CCACACTGTA CACTGAAGAC ACTGAGGTTT TTGAGTCCTG CTGCTGATGA AGGCTGTCAG
    TTTGTGACTG GAATTGTGGG TAAAAACCCA TTACTTCTGA AAGAGCTAAA TCTGAGTGAT
    CGTGAACTAG GAGACACACG AGTGAATCAA CTCTCTGCTC TACTGCAGGA TAAACACTGT
    AAACTCAACA CACTCATTCT TAGTAAATGT GGTGTAACAG TGGAAAGCTG TTCAGCTCTG
    GCTACAGTCC TGAGATCAGA GACCAGTCTA AAAGAGCTTG ACATGAGCAA CAATAATCTG
    CAGGAATCAG GAGTGAAGAA ACTCCAGAGC GGATTAAAAA ACACAAACTG CACACTGGAA
    AAACTCAGTC TTTTAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGAAGCTCAC TGAGTTACTG AAACCAGACT ATACACTGAA GACACTGAGG
    TTTTTGAGTG ATGATGCAGA TGAAGCCTGT ACACATCTGA ATAAAGTTCT GGGTGGAAAC
    CTGTTACTCT TGAGAGAGCT GAATCTGAGT AAACTTGATC TTGGACCATC AGGATTGGAG
    AAACTTGCTG CTGTACTGAA AGATAAACAC TGTAAACTCA ACACACTGAT TCTGAATAAC
    AGCAGTATGT CAGAAGAAGA TTGTCGTGTT TTGGCTGAAG CTTTTAATTC AAACCCATCA
    AATCTGACAG AGCTGAATTT GAGTGGGACT AAACTCACAA ACTCAGGAAT GAAGAGCTTC
    ACCACTCTGT TTCAAAACCA ACAGTGTAGA CTGGAAAAGC TGAAGTTCAG CAGCATGTGT
    ATTACAGCAG AAGGTTGTGC TGCTTTGGCT TCAGCATTTA ACTCAAATCC TTCACACCTG
    ATAGAGCTCA ATCTGAGTGA GAATAAACTA GGAGACTCAG GAGTAACAGA AATCTCCAGT
    CTACTGGCAA ACTCGCAGTG CATGCTGAAG ATACTCGGTC TTTCAGACTG CAGTATCAGT
    GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG
    CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG
    CAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGACGCC
    TGTCGGTTTG TGACTAAAAT TGTGGGTAAA AACCCATTAC TCCTGACAGA ACTGAATCTG
    AGTGATCGTA AACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA
    CACTGTAGAG TCAACACAAT AATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT
    GCTTTAACTT CAGCATTTAT TTTAAACCCA TCACACCTGA TAGAGCTCAA TCTGAGTGGG
    AATAAACTAG GAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TACACAATGC
    CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GAAGCTCACT GATTTACTGC ACAGTAGAAG CTGTAAATTC
    AGATTTCTGA AGAGTTCTGC TGCACAGGAA GCATGTGACT ATCTTACTAA AGTTCTGGGT
    ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT
    GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGAAGAA AATCAAGCTG
    AATAATTGTG AGCTGACAGA GAAAAGCTGT TCACTTCTAG CTGCAGTTCT CTCCTCCAAA
    ACCATCCTGA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAGAA
    ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAAA TACTCAGTCT TTCAGACTGC
    CGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT
    GACTTACTGG AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT
    GATGAAGGCT GTCACTTTGT GACTGAAATG GTGGGTAAAA ACCCATTACT CCTGAAAGAA
    CTGAATCTGA GTGATCGTGT ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTGCTG
    CAGGATAAAC ACTGTCAGAT CCACACACTC ATTCTGTGTT ATTGCAGTAT TACAGAGGAA
    CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC
    CTCAGTGGAA ACAATCTAGG AGACTCTGCA GTGAAAAAAC TCAGTGATTT ACTGATGAAC
    CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTGATTGCA GTATTACAGA GAAACAGTGT
    GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT
    GGGAATAGAC TAGGAGACTC TGGAGCTTAT GGACTTGGCC ATCTGCTGAG CAGTGTGGAT
    TGCAAGCTGA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGGGAAACA GTTGCACTTC
    CTGATTGCAG TTATGTGTAT GGACCCATCA CACCTGAGGG TACTGGACCT CAGTAAGAAT
    CAAATAAAAA GCAGAGGAAT GAACATGCTG TGTGATGTGC TGAATAAACC ACGCTGTAAA
    CTCAAGAGTT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACACTT AACTCAGTCT
    TTGGCTGAGA ACAAAGCACT GGAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG
    GGAGACTCCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG
    TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45491
    Clone ID Related Accession (Same CDS sequence) XM_009298422.2
    Accession Version XM_009298422.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein LOC568664 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG
    TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA
    GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT
    CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT
    AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT
    CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT
    GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG
    TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT
    TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT
    AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG
    CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG
    ACACTGAGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGAAGCTCAC TGATTTACTG CACAGTAGAA GCTGTAAATT CAGATTTCTG
    AAGAGTTCTG CTGCACAGGA AGCATGTGAC TATCTTACTA AAGTTCTGGG TATAAGTCCA
    TTATTACTGA CAGAACTGGA TCTGAGTGAA GATAAATTAG GAGATCTGGA TGGAGAGAAA
    CTCTCTGCTC TTCTGATGGA CTCTCATAGC AAAGTGAAGA AAATCAAGCT GAATAATTGT
    GAGCTGACAG AGAAAAGCTG TTCACTTCTA GCTGCAGTTC TCTCCTCCAA AACCATCCTG
    AAAGAGATGA ACCTGAACAA CAGTCGTCTG CTGGACTCAG GAGTCAGAGA AATCTGTGAG
    GGACTGAAGA ATCCTGTGTG TGAGCTGAAA ATACTCAGTC TTTCAGACTG CCGTATCAGT
    GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG
    CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGACTTACTG
    GAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGAAGGC
    TGTCACTTTG TGACTGAAAT GGTGGGTAAA AACCCATTAC TCCTGAAAGA ACTGAATCTG
    AGTGATCGTG TACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTGCT GCAGGATAAA
    CACTGTCAGA TCCACACACT CATTCTGTGT TATTGCAGTA TTACAGAGGA ACAGTGTGTG
    ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGGA
    AACAATCTAG GAGACTCTGC AGTGAAAAAA CTCAGTGATT TACTGATGAA CCAACAATTC
    AAGCTGGAGA AACTACGTCT GAGTGATTGC AGTATTACAG AGAAACAGTG TGTGATCCTG
    ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAA TGGGAATAGA
    CTAGGAGACT CTGGAGCTTA TGGACTTGGC CATCTGCTGA GCAGTGTGGA TTGCAAGCTG
    AATAAACTAC ATCTGTGTGA TTGCAGTATT ACAGGGAAAC AGTTGCACTT CCTGATTGCA
    GTTATGTGTA TGGACCCATC ACACCTGAGG GTACTGGACC TCAGTAAGAA TCAAATAAAA
    AGCAGAGGAA TGAACATGCT GTGTGATGTG CTGAATAAAC CACGCTGTAA ACTCAAGAGT
    TTAAGACTAA ATGACTGTGG CATTACAGAT GTTACACACT TAACTCAGTC TTTGGCTGAG
    AACAAAGCAC TGGAGTTCTT AAAAGAGCTT GAACTGAGTA AAAATCAAAT GGGAGACTCC
    GAAAAGCAAA AACTCAGTGA CCTGCTGCGA GACTCAAACT GTAAACTGAG GTTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296697.1
    CDS182..7081
    Translation

    Target ORF information:

    RefSeq Version XM_009298422.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298422.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG
    TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA
    GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT
    CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT
    AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT
    CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT
    GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG
    TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT
    TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT
    AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG
    CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG
    ACACTGAGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGAAGCTCAC TGATTTACTG CACAGTAGAA GCTGTAAATT CAGATTTCTG
    AAGAGTTCTG CTGCACAGGA AGCATGTGAC TATCTTACTA AAGTTCTGGG TATAAGTCCA
    TTATTACTGA CAGAACTGGA TCTGAGTGAA GATAAATTAG GAGATCTGGA TGGAGAGAAA
    CTCTCTGCTC TTCTGATGGA CTCTCATAGC AAAGTGAAGA AAATCAAGCT GAATAATTGT
    GAGCTGACAG AGAAAAGCTG TTCACTTCTA GCTGCAGTTC TCTCCTCCAA AACCATCCTG
    AAAGAGATGA ACCTGAACAA CAGTCGTCTG CTGGACTCAG GAGTCAGAGA AATCTGTGAG
    GGACTGAAGA ATCCTGTGTG TGAGCTGAAA ATACTCAGTC TTTCAGACTG CCGTATCAGT
    GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG
    CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGACTTACTG
    GAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGAAGGC
    TGTCACTTTG TGACTGAAAT GGTGGGTAAA AACCCATTAC TCCTGAAAGA ACTGAATCTG
    AGTGATCGTG TACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTGCT GCAGGATAAA
    CACTGTCAGA TCCACACACT CATTCTGTGT TATTGCAGTA TTACAGAGGA ACAGTGTGTG
    ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGGA
    AACAATCTAG GAGACTCTGC AGTGAAAAAA CTCAGTGATT TACTGATGAA CCAACAATTC
    AAGCTGGAGA AACTACGTCT GAGTGATTGC AGTATTACAG AGAAACAGTG TGTGATCCTG
    ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAA TGGGAATAGA
    CTAGGAGACT CTGGAGCTTA TGGACTTGGC CATCTGCTGA GCAGTGTGGA TTGCAAGCTG
    AATAAACTAC ATCTGTGTGA TTGCAGTATT ACAGGGAAAC AGTTGCACTT CCTGATTGCA
    GTTATGTGTA TGGACCCATC ACACCTGAGG GTACTGGACC TCAGTAAGAA TCAAATAAAA
    AGCAGAGGAA TGAACATGCT GTGTGATGTG CTGAATAAAC CACGCTGTAA ACTCAAGAGT
    TTAAGACTAA ATGACTGTGG CATTACAGAT GTTACACACT TAACTCAGTC TTTGGCTGAG
    AACAAAGCAC TGGAGTTCTT AAAAGAGCTT GAACTGAGTA AAAATCAAAT GGGAGACTCC
    GAAAAGCAAA AACTCAGTGA CCTGCTGCGA GACTCAAACT GTAAACTGAG GTTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45492
    Clone ID Related Accession (Same CDS sequence) XM_009298423.2
    Accession Version XM_009298423.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein LOC568664 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA
    AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG
    GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT
    AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA
    TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC
    TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG
    TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC
    CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC
    AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC
    GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT
    CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT
    AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT
    GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT
    GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA
    TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA
    TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG
    GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG
    GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG
    CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC
    AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA
    GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC
    TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC
    ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT
    GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA
    GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG
    CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG
    GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG
    AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG
    TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC
    AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG
    GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC
    TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG
    AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT
    AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG
    TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA
    ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG
    AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296698.1
    CDS182..6913
    Translation

    Target ORF information:

    RefSeq Version XM_009298423.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298423.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA
    AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG
    GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT
    AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA
    TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC
    TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG
    TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC
    CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC
    AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC
    GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT
    CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT
    AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT
    GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT
    GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA
    TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA
    TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG
    GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG
    GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG
    CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC
    AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA
    GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC
    TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC
    ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT
    GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA
    GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG
    CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG
    GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG
    AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG
    TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC
    AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG
    GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC
    TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG
    AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT
    AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG
    TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA
    ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG
    AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45493
    Clone ID Related Accession (Same CDS sequence) XM_009298424.2
    Accession Version XM_009298424.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6726bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein LOC568664 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298424.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG
    TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGTCTTT CAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA TTTACTGCAG
    GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGACGCCTGT
    CGGTTTGTGA CTAAAATTGT GGGTAAAAAC CCATTACTCC TGACAGAACT GAATCTGAGT
    GATCGTAAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC
    TGTAGAGTCA ACACAATAAT GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT
    TTAACTTCAG CATTTATTTT AAACCCATCA CACCTGATAG AGCTCAATCT GAGTGGGAAT
    AAACTAGGAG ACTCAGGAGT AGAGAAGATC TGTCCTCTGC TGGAAAATAC ACAATGCCGA
    TTAGAGAAAC TGAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGAA GCTCACTGAT TTACTGCACA GTAGAAGCTG TAAATTCAGA
    TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA TGTGACTATC TTACTAAAGT TCTGGGTATA
    AGTCCATTAT TACTGACAGA ACTGGATCTG AGTGAAGATA AATTAGGAGA TCTGGATGGA
    GAGAAACTCT CTGCTCTTCT GATGGACTCT CATAGCAAAG TGAAGAAAAT CAAGCTGAAT
    AATTGTGAGC TGACAGAGAA AAGCTGTTCA CTTCTAGCTG CAGTTCTCTC CTCCAAAACC
    ATCCTGAAAG AGATGAACCT GAACAACAGT CGTCTGCTGG ACTCAGGAGT CAGAGAAATC
    TGTGAGGGAC TGAAGAATCC TGTGTGTGAG CTGAAAATAC TCAGTCTTTC AGACTGCCGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAC
    TTACTGGAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGGCTGTC ACTTTGTGAC TGAAATGGTG GGTAAAAACC CATTACTCCT GAAAGAACTG
    AATCTGAGTG ATCGTGTACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTGCTGCAG
    GATAAACACT GTCAGATCCA CACACTCATT CTGTGTTATT GCAGTATTAC AGAGGAACAG
    TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC
    AGTGGAAACA ATCTAGGAGA CTCTGCAGTG AAAAAACTCA GTGATTTACT GATGAACCAA
    CAATTCAAGC TGGAGAAACT ACGTCTGAGT GATTGCAGTA TTACAGAGAA ACAGTGTGTG
    ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAATGGG
    AATAGACTAG GAGACTCTGG AGCTTATGGA CTTGGCCATC TGCTGAGCAG TGTGGATTGC
    AAGCTGAATA AACTACATCT GTGTGATTGC AGTATTACAG GGAAACAGTT GCACTTCCTG
    ATTGCAGTTA TGTGTATGGA CCCATCACAC CTGAGGGTAC TGGACCTCAG TAAGAATCAA
    ATAAAAAGCA GAGGAATGAA CATGCTGTGT GATGTGCTGA ATAAACCACG CTGTAAACTC
    AAGAGTTTAA GACTAAATGA CTGTGGCATT ACAGATGTTA CACACTTAAC TCAGTCTTTG
    GCTGAGAACA AAGCACTGGA GTTCTTAAAA GAGCTTGAAC TGAGTAAAAA TCAAATGGGA
    GACTCCGAAA AGCAAAAACT CAGTGACCTG CTGCGAGACT CAAACTGTAA ACTGAGGTTA
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296699.1
    CDS182..6907
    Translation

    Target ORF information:

    RefSeq Version XM_009298424.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298424.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG
    TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGTCTTT CAGACTGCAG TATCAGTGAA
    GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG
    GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA TTTACTGCAG
    GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGACGCCTGT
    CGGTTTGTGA CTAAAATTGT GGGTAAAAAC CCATTACTCC TGACAGAACT GAATCTGAGT
    GATCGTAAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC
    TGTAGAGTCA ACACAATAAT GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT
    TTAACTTCAG CATTTATTTT AAACCCATCA CACCTGATAG AGCTCAATCT GAGTGGGAAT
    AAACTAGGAG ACTCAGGAGT AGAGAAGATC TGTCCTCTGC TGGAAAATAC ACAATGCCGA
    TTAGAGAAAC TGAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGAA GCTCACTGAT TTACTGCACA GTAGAAGCTG TAAATTCAGA
    TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA TGTGACTATC TTACTAAAGT TCTGGGTATA
    AGTCCATTAT TACTGACAGA ACTGGATCTG AGTGAAGATA AATTAGGAGA TCTGGATGGA
    GAGAAACTCT CTGCTCTTCT GATGGACTCT CATAGCAAAG TGAAGAAAAT CAAGCTGAAT
    AATTGTGAGC TGACAGAGAA AAGCTGTTCA CTTCTAGCTG CAGTTCTCTC CTCCAAAACC
    ATCCTGAAAG AGATGAACCT GAACAACAGT CGTCTGCTGG ACTCAGGAGT CAGAGAAATC
    TGTGAGGGAC TGAAGAATCC TGTGTGTGAG CTGAAAATAC TCAGTCTTTC AGACTGCCGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAC
    TTACTGGAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GAAGGCTGTC ACTTTGTGAC TGAAATGGTG GGTAAAAACC CATTACTCCT GAAAGAACTG
    AATCTGAGTG ATCGTGTACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTGCTGCAG
    GATAAACACT GTCAGATCCA CACACTCATT CTGTGTTATT GCAGTATTAC AGAGGAACAG
    TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC
    AGTGGAAACA ATCTAGGAGA CTCTGCAGTG AAAAAACTCA GTGATTTACT GATGAACCAA
    CAATTCAAGC TGGAGAAACT ACGTCTGAGT GATTGCAGTA TTACAGAGAA ACAGTGTGTG
    ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAATGGG
    AATAGACTAG GAGACTCTGG AGCTTATGGA CTTGGCCATC TGCTGAGCAG TGTGGATTGC
    AAGCTGAATA AACTACATCT GTGTGATTGC AGTATTACAG GGAAACAGTT GCACTTCCTG
    ATTGCAGTTA TGTGTATGGA CCCATCACAC CTGAGGGTAC TGGACCTCAG TAAGAATCAA
    ATAAAAAGCA GAGGAATGAA CATGCTGTGT GATGTGCTGA ATAAACCACG CTGTAAACTC
    AAGAGTTTAA GACTAAATGA CTGTGGCATT ACAGATGTTA CACACTTAAC TCAGTCTTTG
    GCTGAGAACA AAGCACTGGA GTTCTTAAAA GAGCTTGAAC TGAGTAAAAA TCAAATGGGA
    GACTCCGAAA AGCAAAAACT CAGTGACCTG CTGCGAGACT CAAACTGTAA ACTGAGGTTA
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45494
    Clone ID Related Accession (Same CDS sequence) XM_009298425.2
    Accession Version XM_009298425.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein LOC568664 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GACGCCTGTC GGTTTGTGAC TAAAATTGTG GGTAAAAACC CATTACTCCT GACAGAACTG
    AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG
    GATAAACACT GTAGAGTCAA CACAATAATG CTGAATAATA ACAGGATTAC AGCAGAAGGT
    TGTGCTGCTT TAACTTCAGC ATTTATTTTA AACCCATCAC ACCTGATAGA GCTCAATCTG
    AGTGGGAATA AACTAGGAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATACA
    CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGAAG CTCACTGATT TACTGCACAG TAGAAGCTGT
    AAATTCAGAT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GTGACTATCT TACTAAAGTT
    CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT
    CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GAAGAAAATC
    AAGCTGAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC
    TCCAAAACCA TCCTGAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC
    AGAGAAATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAAATACT CAGTCTTTCA
    GACTGCCGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG
    CTCACTGACT TACTGGAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT
    GCTGCTGATG AAGGCTGTCA CTTTGTGACT GAAATGGTGG GTAAAAACCC ATTACTCCTG
    AAAGAACTGA ATCTGAGTGA TCGTGTACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT
    CTGCTGCAGG ATAAACACTG TCAGATCCAC ACACTCATTC TGTGTTATTG CAGTATTACA
    GAGGAACAGT GTGTGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA
    CTGAACCTCA GTGGAAACAA TCTAGGAGAC TCTGCAGTGA AAAAACTCAG TGATTTACTG
    ATGAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTG ATTGCAGTAT TACAGAGAAA
    CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC
    CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTTATGGAC TTGGCCATCT GCTGAGCAGT
    GTGGATTGCA AGCTGAATAA ACTACATCTG TGTGATTGCA GTATTACAGG GAAACAGTTG
    CACTTCCTGA TTGCAGTTAT GTGTATGGAC CCATCACACC TGAGGGTACT GGACCTCAGT
    AAGAATCAAA TAAAAAGCAG AGGAATGAAC ATGCTGTGTG ATGTGCTGAA TAAACCACGC
    TGTAAACTCA AGAGTTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACACTTAACT
    CAGTCTTTGG CTGAGAACAA AGCACTGGAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT
    CAAATGGGAG ACTCCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA
    CTGAGGTTAG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296700.1
    CDS182..6736
    Translation

    Target ORF information:

    RefSeq Version XM_009298425.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298425.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT
    TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT
    GACGCCTGTC GGTTTGTGAC TAAAATTGTG GGTAAAAACC CATTACTCCT GACAGAACTG
    AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG
    GATAAACACT GTAGAGTCAA CACAATAATG CTGAATAATA ACAGGATTAC AGCAGAAGGT
    TGTGCTGCTT TAACTTCAGC ATTTATTTTA AACCCATCAC ACCTGATAGA GCTCAATCTG
    AGTGGGAATA AACTAGGAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATACA
    CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGAAG CTCACTGATT TACTGCACAG TAGAAGCTGT
    AAATTCAGAT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GTGACTATCT TACTAAAGTT
    CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT
    CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GAAGAAAATC
    AAGCTGAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC
    TCCAAAACCA TCCTGAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC
    AGAGAAATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAAATACT CAGTCTTTCA
    GACTGCCGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG
    CTCACTGACT TACTGGAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT
    GCTGCTGATG AAGGCTGTCA CTTTGTGACT GAAATGGTGG GTAAAAACCC ATTACTCCTG
    AAAGAACTGA ATCTGAGTGA TCGTGTACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT
    CTGCTGCAGG ATAAACACTG TCAGATCCAC ACACTCATTC TGTGTTATTG CAGTATTACA
    GAGGAACAGT GTGTGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA
    CTGAACCTCA GTGGAAACAA TCTAGGAGAC TCTGCAGTGA AAAAACTCAG TGATTTACTG
    ATGAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTG ATTGCAGTAT TACAGAGAAA
    CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC
    CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTTATGGAC TTGGCCATCT GCTGAGCAGT
    GTGGATTGCA AGCTGAATAA ACTACATCTG TGTGATTGCA GTATTACAGG GAAACAGTTG
    CACTTCCTGA TTGCAGTTAT GTGTATGGAC CCATCACACC TGAGGGTACT GGACCTCAGT
    AAGAATCAAA TAAAAAGCAG AGGAATGAAC ATGCTGTGTG ATGTGCTGAA TAAACCACGC
    TGTAAACTCA AGAGTTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACACTTAACT
    CAGTCTTTGG CTGAGAACAA AGCACTGGAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT
    CAAATGGGAG ACTCCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA
    CTGAGGTTAG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45495
    Clone ID Related Accession (Same CDS sequence) XM_009298426.2
    Accession Version XM_009298426.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein LOC568664 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTTCA
    GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG
    CTCACTGATT TACTGCAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT
    GCTGCTGATG ACGCCTGTCG GTTTGTGACT AAAATTGTGG GTAAAAACCC ATTACTCCTG
    ACAGAACTGA ATCTGAGTGA TCGTAAACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT
    CTACTGCAGG ATAAACACTG TAGAGTCAAC ACAATAATGC TGAATAATAA CAGGATTACA
    GCAGAAGGTT GTGCTGCTTT AACTTCAGCA TTTATTTTAA ACCCATCACA CCTGATAGAG
    CTCAATCTGA GTGGGAATAA ACTAGGAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG
    GAAAATACAC AATGCCGATT AGAGAAACTG AGTCTTTCAG ACTGCAGTAT CAGTGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGAAGC TCACTGATTT ACTGCACAGT
    AGAAGCTGTA AATTCAGATT TCTGAAGAGT TCTGCTGCAC AGGAAGCATG TGACTATCTT
    ACTAAAGTTC TGGGTATAAG TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGATAAA
    TTAGGAGATC TGGATGGAGA GAAACTCTCT GCTCTTCTGA TGGACTCTCA TAGCAAAGTG
    AAGAAAATCA AGCTGAATAA TTGTGAGCTG ACAGAGAAAA GCTGTTCACT TCTAGCTGCA
    GTTCTCTCCT CCAAAACCAT CCTGAAAGAG ATGAACCTGA ACAACAGTCG TCTGCTGGAC
    TCAGGAGTCA GAGAAATCTG TGAGGGACTG AAGAATCCTG TGTGTGAGCT GAAAATACTC
    AGTCTTTCAG ACTGCCGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGACTT ACTGGAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT
    TTGAGTCCTG CTGCTGATGA AGGCTGTCAC TTTGTGACTG AAATGGTGGG TAAAAACCCA
    TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTGTACTAG GAGACACACG AGTGAATCAA
    CTCTCTGCTC TGCTGCAGGA TAAACACTGT CAGATCCACA CACTCATTCT GTGTTATTGC
    AGTATTACAG AGGAACAGTG TGTGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC
    CTGAGAGAAC TGAACCTCAG TGGAAACAAT CTAGGAGACT CTGCAGTGAA AAAACTCAGT
    GATTTACTGA TGAACCAACA ATTCAAGCTG GAGAAACTAC GTCTGAGTGA TTGCAGTATT
    ACAGAGAAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA
    GAACTGAACC TCAATGGGAA TAGACTAGGA GACTCTGGAG CTTATGGACT TGGCCATCTG
    CTGAGCAGTG TGGATTGCAA GCTGAATAAA CTACATCTGT GTGATTGCAG TATTACAGGG
    AAACAGTTGC ACTTCCTGAT TGCAGTTATG TGTATGGACC CATCACACCT GAGGGTACTG
    GACCTCAGTA AGAATCAAAT AAAAAGCAGA GGAATGAACA TGCTGTGTGA TGTGCTGAAT
    AAACCACGCT GTAAACTCAA GAGTTTAAGA CTAAATGACT GTGGCATTAC AGATGTTACA
    CACTTAACTC AGTCTTTGGC TGAGAACAAA GCACTGGAGT TCTTAAAAGA GCTTGAACTG
    AGTAAAAATC AAATGGGAGA CTCCGAAAAG CAAAAACTCA GTGACCTGCT GCGAGACTCA
    AACTGTAAAC TGAGGTTAGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296701.1
    CDS182..6565
    Translation

    Target ORF information:

    RefSeq Version XM_009298426.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298426.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTTCA
    GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG
    CTCACTGATT TACTGCAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT
    GCTGCTGATG ACGCCTGTCG GTTTGTGACT AAAATTGTGG GTAAAAACCC ATTACTCCTG
    ACAGAACTGA ATCTGAGTGA TCGTAAACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT
    CTACTGCAGG ATAAACACTG TAGAGTCAAC ACAATAATGC TGAATAATAA CAGGATTACA
    GCAGAAGGTT GTGCTGCTTT AACTTCAGCA TTTATTTTAA ACCCATCACA CCTGATAGAG
    CTCAATCTGA GTGGGAATAA ACTAGGAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG
    GAAAATACAC AATGCCGATT AGAGAAACTG AGTCTTTCAG ACTGCAGTAT CAGTGAAGAA
    GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT
    CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGAAGC TCACTGATTT ACTGCACAGT
    AGAAGCTGTA AATTCAGATT TCTGAAGAGT TCTGCTGCAC AGGAAGCATG TGACTATCTT
    ACTAAAGTTC TGGGTATAAG TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGATAAA
    TTAGGAGATC TGGATGGAGA GAAACTCTCT GCTCTTCTGA TGGACTCTCA TAGCAAAGTG
    AAGAAAATCA AGCTGAATAA TTGTGAGCTG ACAGAGAAAA GCTGTTCACT TCTAGCTGCA
    GTTCTCTCCT CCAAAACCAT CCTGAAAGAG ATGAACCTGA ACAACAGTCG TCTGCTGGAC
    TCAGGAGTCA GAGAAATCTG TGAGGGACTG AAGAATCCTG TGTGTGAGCT GAAAATACTC
    AGTCTTTCAG ACTGCCGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGACTT ACTGGAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT
    TTGAGTCCTG CTGCTGATGA AGGCTGTCAC TTTGTGACTG AAATGGTGGG TAAAAACCCA
    TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTGTACTAG GAGACACACG AGTGAATCAA
    CTCTCTGCTC TGCTGCAGGA TAAACACTGT CAGATCCACA CACTCATTCT GTGTTATTGC
    AGTATTACAG AGGAACAGTG TGTGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC
    CTGAGAGAAC TGAACCTCAG TGGAAACAAT CTAGGAGACT CTGCAGTGAA AAAACTCAGT
    GATTTACTGA TGAACCAACA ATTCAAGCTG GAGAAACTAC GTCTGAGTGA TTGCAGTATT
    ACAGAGAAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA
    GAACTGAACC TCAATGGGAA TAGACTAGGA GACTCTGGAG CTTATGGACT TGGCCATCTG
    CTGAGCAGTG TGGATTGCAA GCTGAATAAA CTACATCTGT GTGATTGCAG TATTACAGGG
    AAACAGTTGC ACTTCCTGAT TGCAGTTATG TGTATGGACC CATCACACCT GAGGGTACTG
    GACCTCAGTA AGAATCAAAT AAAAAGCAGA GGAATGAACA TGCTGTGTGA TGTGCTGAAT
    AAACCACGCT GTAAACTCAA GAGTTTAAGA CTAAATGACT GTGGCATTAC AGATGTTACA
    CACTTAACTC AGTCTTTGGC TGAGAACAAA GCACTGGAGT TCTTAAAAGA GCTTGAACTG
    AGTAAAAATC AAATGGGAGA CTCCGAAAAG CAAAAACTCA GTGACCTGCT GCGAGACTCA
    AACTGTAAAC TGAGGTTAGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45496
    Clone ID Related Accession (Same CDS sequence) XM_009298427.2
    Accession Version XM_009298427.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6039bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein LOC568664 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATGCTGAAT
    AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAT TTTAAACCCA
    TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAGAGAAG
    ATCTGTCCTC TGCTGGAAAA TACACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA
    GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT
    CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC
    TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT
    TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC
    AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT
    GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG
    AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG
    GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC
    ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC
    ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA
    GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG
    AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC
    CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT
    GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT
    TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA
    CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG
    TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC
    ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA
    AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC
    CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296702.1
    CDS182..6220
    Translation

    Target ORF information:

    RefSeq Version XM_009298427.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298427.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATGCTGAAT
    AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAT TTTAAACCCA
    TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAGAGAAG
    ATCTGTCCTC TGCTGGAAAA TACACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA
    GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT
    CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC
    TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT
    TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC
    AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT
    GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG
    AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG
    GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC
    ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC
    ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA
    GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG
    AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC
    CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT
    GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT
    TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA
    CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG
    TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC
    ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA
    AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC
    CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45497
    Clone ID Related Accession (Same CDS sequence) XM_009298428.2
    Accession Version XM_009298428.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6039bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein LOC568664 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAATC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGACGCC TGTCGGTTTG TGACTAAAAT TGTGGGTAAA
    AACCCATTAC TCCTGACAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAGAG TCAACACAAT AATGCTGAAT
    AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAT TTTAAACCCA
    TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAGAGAAG
    ATCTGTCCTC TGCTGGAAAA TACACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA
    GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT
    CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC
    TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT
    TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC
    AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT
    GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG
    AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG
    GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC
    ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC
    ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA
    GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG
    AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC
    CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT
    GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT
    TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA
    CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG
    TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC
    ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA
    AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC
    CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296703.1
    CDS182..6220
    Translation

    Target ORF information:

    RefSeq Version XM_009298428.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298428.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAATC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGATTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGACGCC TGTCGGTTTG TGACTAAAAT TGTGGGTAAA
    AACCCATTAC TCCTGACAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAGAG TCAACACAAT AATGCTGAAT
    AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAT TTTAAACCCA
    TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAGAGAAG
    ATCTGTCCTC TGCTGGAAAA TACACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC
    AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC
    CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT
    GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA
    GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT
    CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC
    TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT
    TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC
    AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT
    GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG
    GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT
    CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG
    AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG
    GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC
    ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC
    ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA
    GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG
    AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC
    CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT
    GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT
    TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA
    CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG
    TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC
    ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA
    AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC
    CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45498
    Clone ID Related Accession (Same CDS sequence) XM_009298429.2
    Accession Version XM_009298429.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein LOC568664 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAT
    TTACTGCACA GTAGAAGCTG TAAATTCAGA TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA
    TGTGACTATC TTACTAAAGT TCTGGGTATA AGTCCATTAT TACTGACAGA ACTGGATCTG
    AGTGAAGATA AATTAGGAGA TCTGGATGGA GAGAAACTCT CTGCTCTTCT GATGGACTCT
    CATAGCAAAG TGAAGAAAAT CAAGCTGAAT AATTGTGAGC TGACAGAGAA AAGCTGTTCA
    CTTCTAGCTG CAGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT
    CGTCTGCTGG ACTCAGGAGT CAGAGAAATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG
    CTGAAAATAC TCAGTCTTTC AGACTGCCGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGGAGG ATCCACACTG TACACTGAAG
    ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAAGGCTGTC ACTTTGTGAC TGAAATGGTG
    GGTAAAAACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGTACT AGGAGACACA
    CGAGTGAATC AACTCTCTGC TCTGCTGCAG GATAAACACT GTCAGATCCA CACACTCATT
    CTGTGTTATT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGAAACA ATCTAGGAGA CTCTGCAGTG
    AAAAAACTCA GTGATTTACT GATGAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT
    GATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA
    TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTTATGGA
    CTTGGCCATC TGCTGAGCAG TGTGGATTGC AAGCTGAATA AACTACATCT GTGTGATTGC
    AGTATTACAG GGAAACAGTT GCACTTCCTG ATTGCAGTTA TGTGTATGGA CCCATCACAC
    CTGAGGGTAC TGGACCTCAG TAAGAATCAA ATAAAAAGCA GAGGAATGAA CATGCTGTGT
    GATGTGCTGA ATAAACCACG CTGTAAACTC AAGAGTTTAA GACTAAATGA CTGTGGCATT
    ACAGATGTTA CACACTTAAC TCAGTCTTTG GCTGAGAACA AAGCACTGGA GTTCTTAAAA
    GAGCTTGAAC TGAGTAAAAA TCAAATGGGA GACTCCGAAA AGCAAAAACT CAGTGACCTG
    CTGCGAGACT CAAACTGTAA ACTGAGGTTA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296704.1
    CDS182..6217
    Translation

    Target ORF information:

    RefSeq Version XM_009298429.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298429.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTC AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAT
    TTACTGCACA GTAGAAGCTG TAAATTCAGA TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA
    TGTGACTATC TTACTAAAGT TCTGGGTATA AGTCCATTAT TACTGACAGA ACTGGATCTG
    AGTGAAGATA AATTAGGAGA TCTGGATGGA GAGAAACTCT CTGCTCTTCT GATGGACTCT
    CATAGCAAAG TGAAGAAAAT CAAGCTGAAT AATTGTGAGC TGACAGAGAA AAGCTGTTCA
    CTTCTAGCTG CAGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT
    CGTCTGCTGG ACTCAGGAGT CAGAGAAATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG
    CTGAAAATAC TCAGTCTTTC AGACTGCCGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGGAGG ATCCACACTG TACACTGAAG
    ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAAGGCTGTC ACTTTGTGAC TGAAATGGTG
    GGTAAAAACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGTACT AGGAGACACA
    CGAGTGAATC AACTCTCTGC TCTGCTGCAG GATAAACACT GTCAGATCCA CACACTCATT
    CTGTGTTATT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGAAACA ATCTAGGAGA CTCTGCAGTG
    AAAAAACTCA GTGATTTACT GATGAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT
    GATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA
    TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTTATGGA
    CTTGGCCATC TGCTGAGCAG TGTGGATTGC AAGCTGAATA AACTACATCT GTGTGATTGC
    AGTATTACAG GGAAACAGTT GCACTTCCTG ATTGCAGTTA TGTGTATGGA CCCATCACAC
    CTGAGGGTAC TGGACCTCAG TAAGAATCAA ATAAAAAGCA GAGGAATGAA CATGCTGTGT
    GATGTGCTGA ATAAACCACG CTGTAAACTC AAGAGTTTAA GACTAAATGA CTGTGGCATT
    ACAGATGTTA CACACTTAAC TCAGTCTTTG GCTGAGAACA AAGCACTGGA GTTCTTAAAA
    GAGCTTGAAC TGAGTAAAAA TCAAATGGGA GACTCCGAAA AGCAAAAACT CAGTGACCTG
    CTGCGAGACT CAAACTGTAA ACTGAGGTTA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45501
    Clone ID Related Accession (Same CDS sequence) XM_009298432.2
    Accession Version XM_009298432.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5172bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 12 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298432.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GTCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC
    GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT
    CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT
    AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT
    GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT
    GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA
    TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA
    TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG
    GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG
    GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG
    CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC
    AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA
    GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC
    TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC
    ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT
    GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA
    GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG
    CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG
    GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG
    AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG
    TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC
    AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG
    GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC
    TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG
    AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT
    AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG
    TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA
    ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG
    AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296707.1
    CDS182..5353
    Translation

    Target ORF information:

    RefSeq Version XM_009298432.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298432.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GTCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC
    GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT
    CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT
    AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT
    GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT
    GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA
    TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT
    CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT
    GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA
    TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG
    GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG
    GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG
    CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC
    AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA
    GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC
    TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA
    CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC
    ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT
    GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA
    GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG
    CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG
    GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG
    AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG
    TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC
    AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG
    GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC
    TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG
    AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT
    AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG
    TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA
    ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG
    AGGTTAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45502
    Clone ID Related Accession (Same CDS sequence) XM_009298433.2
    Accession Version XM_009298433.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298433.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT GAGCTGACAG AGAAAAGCTG TTCACTTCTA
    GCTGCAGTTC TCTCCTCCAA AACCATCCTG AAAGAGATGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA AATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAA
    ATACTCAGTC TTTCAGACTG CCGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGACTTACTG GAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCACTTTG TGACTGAAAT GGTGGGTAAA
    AACCCATTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTG TACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTGCT GCAGGATAAA CACTGTCAGA TCCACACACT CATTCTGTGT
    TATTGCAGTA TTACAGAGGA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA
    TCACACCTGA GAGAACTGAA CCTCAGTGGA AACAATCTAG GAGACTCTGC AGTGAAAAAA
    CTCAGTGATT TACTGATGAA CCAACAATTC AAGCTGGAGA AACTACGTCT GAGTGATTGC
    AGTATTACAG AGAAACAGTG TGTGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC
    CTGAGAGAAC TGAACCTCAA TGGGAATAGA CTAGGAGACT CTGGAGCTTA TGGACTTGGC
    CATCTGCTGA GCAGTGTGGA TTGCAAGCTG AATAAACTAC ATCTGTGTGA TTGCAGTATT
    ACAGGGAAAC AGTTGCACTT CCTGATTGCA GTTATGTGTA TGGACCCATC ACACCTGAGG
    GTACTGGACC TCAGTAAGAA TCAAATAAAA AGCAGAGGAA TGAACATGCT GTGTGATGTG
    CTGAATAAAC CACGCTGTAA ACTCAAGAGT TTAAGACTAA ATGACTGTGG CATTACAGAT
    GTTACACACT TAACTCAGTC TTTGGCTGAG AACAAAGCAC TGGAGTTCTT AAAAGAGCTT
    GAACTGAGTA AAAATCAAAT GGGAGACTCC GAAAAGCAAA AACTCAGTGA CCTGCTGCGA
    GACTCAAACT GTAAACTGAG GTTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296708.1
    CDS182..5191
    Translation

    Target ORF information:

    RefSeq Version XM_009298433.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298433.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT GAGCTGACAG AGAAAAGCTG TTCACTTCTA
    GCTGCAGTTC TCTCCTCCAA AACCATCCTG AAAGAGATGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA AATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAA
    ATACTCAGTC TTTCAGACTG CCGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGACTTACTG GAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCACTTTG TGACTGAAAT GGTGGGTAAA
    AACCCATTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTG TACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTGCT GCAGGATAAA CACTGTCAGA TCCACACACT CATTCTGTGT
    TATTGCAGTA TTACAGAGGA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA
    TCACACCTGA GAGAACTGAA CCTCAGTGGA AACAATCTAG GAGACTCTGC AGTGAAAAAA
    CTCAGTGATT TACTGATGAA CCAACAATTC AAGCTGGAGA AACTACGTCT GAGTGATTGC
    AGTATTACAG AGAAACAGTG TGTGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC
    CTGAGAGAAC TGAACCTCAA TGGGAATAGA CTAGGAGACT CTGGAGCTTA TGGACTTGGC
    CATCTGCTGA GCAGTGTGGA TTGCAAGCTG AATAAACTAC ATCTGTGTGA TTGCAGTATT
    ACAGGGAAAC AGTTGCACTT CCTGATTGCA GTTATGTGTA TGGACCCATC ACACCTGAGG
    GTACTGGACC TCAGTAAGAA TCAAATAAAA AGCAGAGGAA TGAACATGCT GTGTGATGTG
    CTGAATAAAC CACGCTGTAA ACTCAAGAGT TTAAGACTAA ATGACTGTGG CATTACAGAT
    GTTACACACT TAACTCAGTC TTTGGCTGAG AACAAAGCAC TGGAGTTCTT AAAAGAGCTT
    GAACTGAGTA AAAATCAAAT GGGAGACTCC GAAAAGCAAA AACTCAGTGA CCTGCTGCGA
    GACTCAAACT GTAAACTGAG GTTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45505
    Clone ID Related Accession (Same CDS sequence) XM_009298436.1
    Accession Version XM_009298436.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3480bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein LOC568664 isoform X16
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGACCCTC ATAGCAAAGT GGAGAAAATC AAGCTGAATA ATTGTAAGCT GCTTGAGAAA 
    AGCTCATCAG TTTTAGCAAC AGTTCTGTCT TCCAAAACCA TCCTGAAAGA GCTGAACCTG
    AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAGAGATCT GTGAGGGACT GAAGAATCCT
    GTGTGTGAGC TGAAGATACT CAAGCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGAGT TACTGCAGGA TCCACACTGT
    ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AAGGCTGTCA GTTTGTGACT
    GGAATTGTGG GTAAAAACCC ATTACTTCTG AAAGAGCTAA ATCTGAGTGA TCGTGAACTA
    GGAGACACAC GAGTGAATCA ACTCTCTGCT CTACTGCAGG ATAAACACTG TAAACTCAAC
    ACACTCATTC TTAGTAAATG TGGTGTAACA GTGGAAAGCT GTTCAGCTCT GGCTACAGTC
    CTGAGATCAG AGACCAGTCT AAAAGAGCTT GACATGAGCA ACAATAATCT GCAGGAATCA
    GGAGTGAAGA AACTCCAGAG CGGATTAAAA AACACAAACT GCACACTGGA AAAACTCAGT
    CTTTTAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA
    AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG
    AAGAAGCTCA CTGAGTTACT GAAACCAGAC TATACACTGA AGACACTGAG GTTTTTGAGT
    GATGATGCAG ATGAAGCCTG TACACATCTG AATAAAGTTC TGGGTGGAAA CCTGTTACTC
    TTGAGAGAGC TGAATCTGAG TAAACTTGAT CTTGGACCAT CAGGATTGGA GAAACTTGCT
    GCTGTACTGA AAGATAAACA CTGTAAACTC AACACACTGA TTCTGAATAA CAGCAGTATG
    TCAGAAGAAG ATTGTCGTGT TTTGGCTGAA GCTTTTAATT CAAACCCATC AAATCTGACA
    GAGCTGAATT TGAGTGGGAC TAAACTCACA AACTCAGGAA TGAAGAGCTT CACCACTCTG
    TTTCAAAACC AACAGTGTAG ACTGGAAAAG CTGAAGTTCA GCAGCATGTG TATTACAGCA
    GAAGGTTGTG CTGCTTTGGC TTCAGCATTT AACTCAAATC CTTCACACCT GATAGAGCTC
    AATCTGAGTG AGAATAAACT AGGAGACTCA GGAGTAACAG AAATCTCCAG TCTACTGGCA
    AACTCGCAGT GCATGCTGAA GATACTCGGT CTTTCAGACT GCAGTATCAG TGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTCA CTGATTTACT GCAGGATCCA
    CACTGTACAC TGAAGACACT GAGGTTTTTG AGTCCTGCTG CTGATGACGC CTGTCGGTTT
    GTGACTAAAA TTGTGGGTAA AAACCCATTA CTCCTGACAG AACTGAATCT GAGTGATCGT
    AAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTAGA
    GTCAACACAA TAATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTAACT
    TCAGCATTTA TTTTAAACCC ATCACACCTG ATAGAGCTCA ATCTGAGTGG GAATAAACTA
    GGAGACTCAG GAGTAGAGAA GATCTGTCCT CTGCTGGAAA ATACACAATG CCGATTAGAG
    AAACTGAGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGAAGCTCAC TGATTTACTG CACAGTAGAA GCTGTAAATT CAGATTTCTG
    AAGAGTTCTG CTGCACAGGA AGCATGTGAC TATCTTACTA AAGTTCTGGG TATAAGTCCA
    TTATTACTGA CAGAACTGGA TCTGAGTGAA GATAAATTAG GAGATCTGGA TGGAGAGAAA
    CTCTCTGCTC TTCTGATGGA CTCTCATAGC AAAGTGAAGA AAATCAAGCT GAATAATTGT
    GAGCTGACAG AGAAAAGCTG TTCACTTCTA GCTGCAGTTC TCTCCTCCAA AACCATCCTG
    AAAGAGATGA ACCTGAACAA CAGTCGTCTG CTGGACTCAG GAGTCAGAGA AATCTGTGAG
    GGACTGAAGA ATCCTGTGTG TGAGCTGAAA ATACTCAGTC TTTCAGACTG CCGTATCAGT
    GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG
    CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGACTTACTG
    GAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGAAGGC
    TGTCACTTTG TGACTGAAAT GGTGGGTAAA AACCCATTAC TCCTGAAAGA ACTGAATCTG
    AGTGATCGTG TACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTGCT GCAGGATAAA
    CACTGTCAGA TCCACACACT CATTCTGTGT TATTGCAGTA TTACAGAGGA ACAGTGTGTG
    ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGGA
    AACAATCTAG GAGACTCTGC AGTGAAAAAA CTCAGTGATT TACTGATGAA CCAACAATTC
    AAGCTGGAGA AACTACGTCT GAGTGATTGC AGTATTACAG AGAAACAGTG TGTGATCCTG
    ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAA TGGGAATAGA
    CTAGGAGACT CTGGAGCTTA TGGACTTGGC CATCTGCTGA GCAGTGTGGA TTGCAAGCTG
    AATAAACTAC ATCTGTGTGA TTGCAGTATT ACAGGGAAAC AGTTGCACTT CCTGATTGCA
    GTTATGTGTA TGGACCCATC ACACCTGAGG GTACTGGACC TCAGTAAGAA TCAAATAAAA
    AGCAGAGGAA TGAACATGCT GTGTGATGTG CTGAATAAAC CACGCTGTAA ACTCAAGAGT
    TTAAGACTAA ATGACTGTGG CATTACAGAT GTTACACACT TAACTCAGTC TTTGGCTGAG
    AACAAAGCAC TGGAGTTCTT AAAAGAGCTT GAACTGAGTA AAAATCAAAT GGGAGACTCC
    GAAAAGCAAA AACTCAGTGA CCTGCTGCGA GACTCAAACT GTAAACTGAG GTTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296711.1
    CDS139..3618
    Translation

    Target ORF information:

    RefSeq Version XM_009298436.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298436.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGACCCTC ATAGCAAAGT GGAGAAAATC AAGCTGAATA ATTGTAAGCT GCTTGAGAAA 
    AGCTCATCAG TTTTAGCAAC AGTTCTGTCT TCCAAAACCA TCCTGAAAGA GCTGAACCTG
    AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAGAGATCT GTGAGGGACT GAAGAATCCT
    GTGTGTGAGC TGAAGATACT CAAGCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGAGT TACTGCAGGA TCCACACTGT
    ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AAGGCTGTCA GTTTGTGACT
    GGAATTGTGG GTAAAAACCC ATTACTTCTG AAAGAGCTAA ATCTGAGTGA TCGTGAACTA
    GGAGACACAC GAGTGAATCA ACTCTCTGCT CTACTGCAGG ATAAACACTG TAAACTCAAC
    ACACTCATTC TTAGTAAATG TGGTGTAACA GTGGAAAGCT GTTCAGCTCT GGCTACAGTC
    CTGAGATCAG AGACCAGTCT AAAAGAGCTT GACATGAGCA ACAATAATCT GCAGGAATCA
    GGAGTGAAGA AACTCCAGAG CGGATTAAAA AACACAAACT GCACACTGGA AAAACTCAGT
    CTTTTAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA
    AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG
    AAGAAGCTCA CTGAGTTACT GAAACCAGAC TATACACTGA AGACACTGAG GTTTTTGAGT
    GATGATGCAG ATGAAGCCTG TACACATCTG AATAAAGTTC TGGGTGGAAA CCTGTTACTC
    TTGAGAGAGC TGAATCTGAG TAAACTTGAT CTTGGACCAT CAGGATTGGA GAAACTTGCT
    GCTGTACTGA AAGATAAACA CTGTAAACTC AACACACTGA TTCTGAATAA CAGCAGTATG
    TCAGAAGAAG ATTGTCGTGT TTTGGCTGAA GCTTTTAATT CAAACCCATC AAATCTGACA
    GAGCTGAATT TGAGTGGGAC TAAACTCACA AACTCAGGAA TGAAGAGCTT CACCACTCTG
    TTTCAAAACC AACAGTGTAG ACTGGAAAAG CTGAAGTTCA GCAGCATGTG TATTACAGCA
    GAAGGTTGTG CTGCTTTGGC TTCAGCATTT AACTCAAATC CTTCACACCT GATAGAGCTC
    AATCTGAGTG AGAATAAACT AGGAGACTCA GGAGTAACAG AAATCTCCAG TCTACTGGCA
    AACTCGCAGT GCATGCTGAA GATACTCGGT CTTTCAGACT GCAGTATCAG TGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTCA CTGATTTACT GCAGGATCCA
    CACTGTACAC TGAAGACACT GAGGTTTTTG AGTCCTGCTG CTGATGACGC CTGTCGGTTT
    GTGACTAAAA TTGTGGGTAA AAACCCATTA CTCCTGACAG AACTGAATCT GAGTGATCGT
    AAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTAGA
    GTCAACACAA TAATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTAACT
    TCAGCATTTA TTTTAAACCC ATCACACCTG ATAGAGCTCA ATCTGAGTGG GAATAAACTA
    GGAGACTCAG GAGTAGAGAA GATCTGTCCT CTGCTGGAAA ATACACAATG CCGATTAGAG
    AAACTGAGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGAAGCTCAC TGATTTACTG CACAGTAGAA GCTGTAAATT CAGATTTCTG
    AAGAGTTCTG CTGCACAGGA AGCATGTGAC TATCTTACTA AAGTTCTGGG TATAAGTCCA
    TTATTACTGA CAGAACTGGA TCTGAGTGAA GATAAATTAG GAGATCTGGA TGGAGAGAAA
    CTCTCTGCTC TTCTGATGGA CTCTCATAGC AAAGTGAAGA AAATCAAGCT GAATAATTGT
    GAGCTGACAG AGAAAAGCTG TTCACTTCTA GCTGCAGTTC TCTCCTCCAA AACCATCCTG
    AAAGAGATGA ACCTGAACAA CAGTCGTCTG CTGGACTCAG GAGTCAGAGA AATCTGTGAG
    GGACTGAAGA ATCCTGTGTG TGAGCTGAAA ATACTCAGTC TTTCAGACTG CCGTATCAGT
    GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG
    CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGACTTACTG
    GAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGAAGGC
    TGTCACTTTG TGACTGAAAT GGTGGGTAAA AACCCATTAC TCCTGAAAGA ACTGAATCTG
    AGTGATCGTG TACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTGCT GCAGGATAAA
    CACTGTCAGA TCCACACACT CATTCTGTGT TATTGCAGTA TTACAGAGGA ACAGTGTGTG
    ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGGA
    AACAATCTAG GAGACTCTGC AGTGAAAAAA CTCAGTGATT TACTGATGAA CCAACAATTC
    AAGCTGGAGA AACTACGTCT GAGTGATTGC AGTATTACAG AGAAACAGTG TGTGATCCTG
    ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAA TGGGAATAGA
    CTAGGAGACT CTGGAGCTTA TGGACTTGGC CATCTGCTGA GCAGTGTGGA TTGCAAGCTG
    AATAAACTAC ATCTGTGTGA TTGCAGTATT ACAGGGAAAC AGTTGCACTT CCTGATTGCA
    GTTATGTGTA TGGACCCATC ACACCTGAGG GTACTGGACC TCAGTAAGAA TCAAATAAAA
    AGCAGAGGAA TGAACATGCT GTGTGATGTG CTGAATAAAC CACGCTGTAA ACTCAAGAGT
    TTAAGACTAA ATGACTGTGG CATTACAGAT GTTACACACT TAACTCAGTC TTTGGCTGAG
    AACAAAGCAC TGGAGTTCTT AAAAGAGCTT GAACTGAGTA AAAATCAAAT GGGAGACTCC
    GAAAAGCAAA AACTCAGTGA CCTGCTGCGA GACTCAAACT GTAAACTGAG GTTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53641
    Clone ID Related Accession (Same CDS sequence) XM_017354578.1
    Accession Version XM_017354578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7245bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product uncharacterized protein LOC568664 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG
    TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA
    GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT
    CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT
    AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT
    CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT
    GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG
    TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT
    TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT
    AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG
    CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG
    ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GACGCCTGTC GGTTTGTGAC TAAAATTGTG
    GGTAAAAACC CATTACTCCT GACAGAACTG AATCTGAGTG ATCGTAAACT AGGAGACACA
    CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GTAGAGTCAA CACAATAATG
    CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTATTTTA
    AACCCATCAC ACCTGATAGA GCTCAATCTG AGTGGGAATA AACTAGGAGA CTCAGGAGTA
    GAGAAGATCT GTCCTCTGCT GGAAAATACA CAATGCCGAT TAGAGAAACT GAGTCTTTCA
    GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGAAG
    CTCACTGATT TACTGCACAG TAGAAGCTGT AAATTCAGAT TTCTGAAGAG TTCTGCTGCA
    CAGGAAGCAT GTGACTATCT TACTAAAGTT CTGGGTATAA GTCCATTATT ACTGACAGAA
    CTGGATCTGA GTGAAGATAA ATTAGGAGAT CTGGATGGAG AGAAACTCTC TGCTCTTCTG
    ATGGACTCTC ATAGCAAAGT GAAGAAAATC AAGCTGAATA ATTGTGAGCT GACAGAGAAA
    AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC TCCAAAACCA TCCTGAAAGA GATGAACCTG
    AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAGAAATCT GTGAGGGACT GAAGAATCCT
    GTGTGTGAGC TGAAAATACT CAGTCTTTCA GACTGCCGTA TCAGTGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGACT TACTGGAGGA TCCACACTGT
    ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AAGGCTGTCA CTTTGTGACT
    GAAATGGTGG GTAAAAACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGTACTA
    GGAGACACAC GAGTGAATCA ACTCTCTGCT CTGCTGCAGG ATAAACACTG TCAGATCCAC
    ACACTCATTC TGTGTTATTG CAGTATTACA GAGGAACAGT GTGTGATCCT GACTTCAGCT
    CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGAAACAA TCTAGGAGAC
    TCTGCAGTGA AAAAACTCAG TGATTTACTG ATGAACCAAC AATTCAAGCT GGAGAAACTA
    CGTCTGAGTG ATTGCAGTAT TACAGAGAAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAATGGGA ATAGACTAGG AGACTCTGGA
    GCTTATGGAC TTGGCCATCT GCTGAGCAGT GTGGATTGCA AGCTGAATAA ACTACATCTG
    TGTGATTGCA GTATTACAGG GAAACAGTTG CACTTCCTGA TTGCAGTTAT GTGTATGGAC
    CCATCACACC TGAGGGTACT GGACCTCAGT AAGAATCAAA TAAAAAGCAG AGGAATGAAC
    ATGCTGTGTG ATGTGCTGAA TAAACCACGC TGTAAACTCA AGAGTTTAAG ACTAAATGAC
    TGTGGCATTA CAGATGTTAC ACACTTAACT CAGTCTTTGG CTGAGAACAA AGCACTGGAG
    TTCTTAAAAG AGCTTGAACT GAGTAAAAAT CAAATGGGAG ACTCCGAAAA GCAAAAACTC
    AGTGACCTGC TGCGAGACTC AAACTGTAAA CTGAGGTTAG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210067.1
    CDS182..7426
    Translation

    Target ORF information:

    RefSeq Version XM_017354578.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG
    TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA
    GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT
    CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT
    AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT
    CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT
    GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG
    TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT
    TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT
    AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG
    CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG
    ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GACGCCTGTC GGTTTGTGAC TAAAATTGTG
    GGTAAAAACC CATTACTCCT GACAGAACTG AATCTGAGTG ATCGTAAACT AGGAGACACA
    CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GTAGAGTCAA CACAATAATG
    CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTATTTTA
    AACCCATCAC ACCTGATAGA GCTCAATCTG AGTGGGAATA AACTAGGAGA CTCAGGAGTA
    GAGAAGATCT GTCCTCTGCT GGAAAATACA CAATGCCGAT TAGAGAAACT GAGTCTTTCA
    GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT
    TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGAAG
    CTCACTGATT TACTGCACAG TAGAAGCTGT AAATTCAGAT TTCTGAAGAG TTCTGCTGCA
    CAGGAAGCAT GTGACTATCT TACTAAAGTT CTGGGTATAA GTCCATTATT ACTGACAGAA
    CTGGATCTGA GTGAAGATAA ATTAGGAGAT CTGGATGGAG AGAAACTCTC TGCTCTTCTG
    ATGGACTCTC ATAGCAAAGT GAAGAAAATC AAGCTGAATA ATTGTGAGCT GACAGAGAAA
    AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC TCCAAAACCA TCCTGAAAGA GATGAACCTG
    AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAGAAATCT GTGAGGGACT GAAGAATCCT
    GTGTGTGAGC TGAAAATACT CAGTCTTTCA GACTGCCGTA TCAGTGAAGA AGGTTATAAA
    GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA
    AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGACT TACTGGAGGA TCCACACTGT
    ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AAGGCTGTCA CTTTGTGACT
    GAAATGGTGG GTAAAAACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGTACTA
    GGAGACACAC GAGTGAATCA ACTCTCTGCT CTGCTGCAGG ATAAACACTG TCAGATCCAC
    ACACTCATTC TGTGTTATTG CAGTATTACA GAGGAACAGT GTGTGATCCT GACTTCAGCT
    CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGAAACAA TCTAGGAGAC
    TCTGCAGTGA AAAAACTCAG TGATTTACTG ATGAACCAAC AATTCAAGCT GGAGAAACTA
    CGTCTGAGTG ATTGCAGTAT TACAGAGAAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA
    TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAATGGGA ATAGACTAGG AGACTCTGGA
    GCTTATGGAC TTGGCCATCT GCTGAGCAGT GTGGATTGCA AGCTGAATAA ACTACATCTG
    TGTGATTGCA GTATTACAGG GAAACAGTTG CACTTCCTGA TTGCAGTTAT GTGTATGGAC
    CCATCACACC TGAGGGTACT GGACCTCAGT AAGAATCAAA TAAAAAGCAG AGGAATGAAC
    ATGCTGTGTG ATGTGCTGAA TAAACCACGC TGTAAACTCA AGAGTTTAAG ACTAAATGAC
    TGTGGCATTA CAGATGTTAC ACACTTAACT CAGTCTTTGG CTGAGAACAA AGCACTGGAG
    TTCTTAAAAG AGCTTGAACT GAGTAAAAAT CAAATGGGAG ACTCCGAAAA GCAAAAACTC
    AGTGACCTGC TGCGAGACTC AAACTGTAAA CTGAGGTTAG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53642
    Clone ID Related Accession (Same CDS sequence) XM_017354579.1
    Accession Version XM_017354579.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product uncharacterized protein LOC568664 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG
    TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA
    GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT
    CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT
    AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT
    CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT
    GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG
    TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT
    TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT
    AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG
    CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG
    ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAAGGCTGTC ACTTTGTGAC TGAAATGGTG
    GGTAAAAACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGTACT AGGAGACACA
    CGAGTGAATC AACTCTCTGC TCTGCTGCAG GATAAACACT GTCAGATCCA CACACTCATT
    CTGTGTTATT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGAAACA ATCTAGGAGA CTCTGCAGTG
    AAAAAACTCA GTGATTTACT GATGAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT
    GATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA
    TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTTATGGA
    CTTGGCCATC TGCTGAGCAG TGTGGATTGC AAGCTGAATA AACTACATCT GTGTGATTGC
    AGTATTACAG GGAAACAGTT GCACTTCCTG ATTGCAGTTA TGTGTATGGA CCCATCACAC
    CTGAGGGTAC TGGACCTCAG TAAGAATCAA ATAAAAAGCA GAGGAATGAA CATGCTGTGT
    GATGTGCTGA ATAAACCACG CTGTAAACTC AAGAGTTTAA GACTAAATGA CTGTGGCATT
    ACAGATGTTA CACACTTAAC TCAGTCTTTG GCTGAGAACA AAGCACTGGA GTTCTTAAAA
    GAGCTTGAAC TGAGTAAAAA TCAAATGGGA GACTCCGAAA AGCAAAAACT CAGTGACCTG
    CTGCGAGACT CAAACTGTAA ACTGAGGTTA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210068.1
    CDS182..6397
    Translation

    Target ORF information:

    RefSeq Version XM_017354579.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354579.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC
    AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA
    GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA
    CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA
    GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT
    CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT
    GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT
    TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC
    AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA
    CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT
    TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA
    GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC
    AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA
    GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG
    CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG
    ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT
    CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA
    TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG
    AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA
    AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG
    AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT
    AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC
    AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC
    CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT
    ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG
    ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG
    TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA
    GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT
    CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT
    AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT
    CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT
    GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG
    TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT
    TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT
    AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG
    CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT
    TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT
    GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG
    ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAAGGCTGTC ACTTTGTGAC TGAAATGGTG
    GGTAAAAACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGTACT AGGAGACACA
    CGAGTGAATC AACTCTCTGC TCTGCTGCAG GATAAACACT GTCAGATCCA CACACTCATT
    CTGTGTTATT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGAAACA ATCTAGGAGA CTCTGCAGTG
    AAAAAACTCA GTGATTTACT GATGAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT
    GATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA
    TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTTATGGA
    CTTGGCCATC TGCTGAGCAG TGTGGATTGC AAGCTGAATA AACTACATCT GTGTGATTGC
    AGTATTACAG GGAAACAGTT GCACTTCCTG ATTGCAGTTA TGTGTATGGA CCCATCACAC
    CTGAGGGTAC TGGACCTCAG TAAGAATCAA ATAAAAAGCA GAGGAATGAA CATGCTGTGT
    GATGTGCTGA ATAAACCACG CTGTAAACTC AAGAGTTTAA GACTAAATGA CTGTGGCATT
    ACAGATGTTA CACACTTAAC TCAGTCTTTG GCTGAGAACA AAGCACTGGA GTTCTTAAAA
    GAGCTTGAAC TGAGTAAAAA TCAAATGGGA GACTCCGAAA AGCAAAAACT CAGTGACCTG
    CTGCGAGACT CAAACTGTAA ACTGAGGTTA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53643
    Clone ID Related Accession (Same CDS sequence) XM_017354580.1
    Accession Version XM_017354580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 12 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GTTTAAGCAG CTGTTCTCTT
    ACAGATGAAA GTTATTCTGC TCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA
    GAACTGGACC TCAGTCAGAA TAAACTGGGA GACTCTGCAG TGAAAAACCT CAGTGATTTA
    CTGATGAAGC CTCAATTTAA GCTGGAGAAA CTACATCTGT ATGAGTGCAG TATTACAGAG
    AAACAGTGTG AGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAGTT GGAGTCAAAT AAGAAACACA GGACTGAATC TCTTCTGTGA TGTGCTGAAG
    GATTCACGCT GTAAACTGGA GACACTGAGA TTGTTTGGTT GTGGCATCTC AGACGTTTCC
    TCTTTAACTC AGTGTTTGAT GAACACAAAA GCCCTGCAGT TTCTAAAAGA GCTTGATCTG
    AGATTCAATC ACATTGGAGA CTCAAAGCAA AAGCTCATTG AAGGGATACG AGACTCAAAC
    TGTAAACTGA GCTTCAGAAT AGAGGATCAG TTGCCGAAGC CTGATGACTC GAGATGTTGT
    ATATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210069.1
    CDS182..3850
    Translation

    Target ORF information:

    RefSeq Version XM_017354580.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
    GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC
    ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC
    TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG
    AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT
    ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG
    AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT
    CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT
    GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC
    TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT
    CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA
    CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA
    GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG
    CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG
    ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC
    AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA
    GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA
    GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC
    AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG
    TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC
    CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG
    TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT
    CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC
    AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA
    GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA
    AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA
    TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA
    GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT
    TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG
    TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA
    CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC
    AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC
    TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT
    GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GTTTAAGCAG CTGTTCTCTT
    ACAGATGAAA GTTATTCTGC TCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA
    GAACTGGACC TCAGTCAGAA TAAACTGGGA GACTCTGCAG TGAAAAACCT CAGTGATTTA
    CTGATGAAGC CTCAATTTAA GCTGGAGAAA CTACATCTGT ATGAGTGCAG TATTACAGAG
    AAACAGTGTG AGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG
    AACCTCAGTT GGAGTCAAAT AAGAAACACA GGACTGAATC TCTTCTGTGA TGTGCTGAAG
    GATTCACGCT GTAAACTGGA GACACTGAGA TTGTTTGGTT GTGGCATCTC AGACGTTTCC
    TCTTTAACTC AGTGTTTGAT GAACACAAAA GCCCTGCAGT TTCTAAAAGA GCTTGATCTG
    AGATTCAATC ACATTGGAGA CTCAAAGCAA AAGCTCATTG AAGGGATACG AGACTCAAAC
    TGTAAACTGA GCTTCAGAAT AGAGGATCAG TTGCCGAAGC CTGATGACTC GAGATGTTGT
    ATATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.