LOC568664 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC568664
Entrez Gene ID	568664
Full Name	NACHT, LRR and PYD domains-containing protein 12
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009298425.2	XP_009296700.1	uncharacterized protein LOC568664 isoform X7
XM_009298429.2	XP_009296704.1	uncharacterized protein LOC568664 isoform X12
XM_009298436.1	XP_009296711.1	uncharacterized protein LOC568664 isoform X16
XM_021471031.1	XP_021326706.1	uncharacterized protein LOC568664 isoform X11
XM_009298432.2	XP_009296707.1	NACHT, LRR and PYD domains-containing protein 12 isoform X19
XM_009298422.2	XP_009296697.1	uncharacterized protein LOC568664 isoform X4
XM_017354578.1	XP_017210067.1	uncharacterized protein LOC568664 isoform X2
XM_021471022.1	XP_021326697.1	uncharacterized protein LOC568664 isoform X4
XM_021471034.1	XP_021326709.1	uncharacterized protein LOC568664 isoform X14
XM_021471035.1	XP_021326710.1	uncharacterized protein LOC568664 isoform X15
XM_021471037.1	XP_021326712.1	NLR family CARD domain-containing protein 3 isoform X17
XM_021471039.1	XP_021326714.1	NACHT, LRR and PYD domains-containing protein 3 isoform X20
XM_021471020.1	XP_021326695.1	uncharacterized protein LOC568664 isoform X1
XM_021471023.1	XP_021326698.1	uncharacterized protein LOC568664 isoform X4
XM_021471024.1	XP_021326699.1	uncharacterized protein LOC568664 isoform X5
XM_021471025.1	XP_021326700.1	uncharacterized protein LOC568664 isoform X6
XM_021471026.1	XP_021326701.1	uncharacterized protein LOC568664 isoform X7
XM_021471029.1	XP_021326704.1	uncharacterized protein LOC568664 isoform X9
XM_021471038.1	XP_021326713.1	NLR family CARD domain-containing protein 3 isoform X18
XM_009298421.2	XP_009296696.1	uncharacterized protein LOC568664 isoform X3
XM_009298424.2	XP_009296699.1	uncharacterized protein LOC568664 isoform X6
XM_017354579.1	XP_017210068.1	uncharacterized protein LOC568664 isoform X9
XM_021471028.1	XP_021326703.1	protein NLRC5 isoform X8
XM_021471030.1	XP_021326705.1	uncharacterized protein LOC568664 isoform X10
XM_021471032.1	XP_021326707.1	uncharacterized protein LOC568664 isoform X12
XM_021471033.1	XP_021326708.1	uncharacterized protein LOC568664 isoform X13
XM_017354580.1	XP_017210069.1	NACHT, LRR and PYD domains-containing protein 12 isoform X21
XM_009298420.2	XP_009296695.1	uncharacterized protein LOC568664 isoform X2
XM_009298423.2	XP_009296698.1	uncharacterized protein LOC568664 isoform X5
XM_009298426.2	XP_009296701.1	uncharacterized protein LOC568664 isoform X8
XM_009298427.2	XP_009296702.1	uncharacterized protein LOC568664 isoform X10
XM_009298428.2	XP_009296703.1	uncharacterized protein LOC568664 isoform X11
XM_009298433.2	XP_009296708.1	NACHT, LRR and PYD domains-containing protein 3 isoform X14
XM_021471021.1	XP_021326696.1	uncharacterized protein LOC568664 isoform X3
XM_021471036.1	XP_021326711.1	uncharacterized protein LOC568664 isoform X16

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC565414	XP_693785.6
Danio rerio (zebrafish)	LOC558375	XP_686669.1
Danio rerio (zebrafish)	ftr29	XP_003198034.1
Danio rerio (zebrafish)	ftr82	NP_001068571.1
Danio rerio (zebrafish)	LOC560422	XP_688915.1
Danio rerio (zebrafish)	LOC100000832	XP_001340954.2
Danio rerio (zebrafish)	LOC100006636	XP_005172385.1
Danio rerio (zebrafish)	ftr73	XP_005157866.1
Danio rerio (zebrafish)	ftr11	XP_003197971.1
Danio rerio (zebrafish)	LOC101882560	XP_005163612.1
Danio rerio (zebrafish)	ftr67	NP_001034808.2
Danio rerio (zebrafish)	ftr34	XP_005163669.1
Danio rerio (zebrafish)	ftr37	XP_696445.3
Danio rerio (zebrafish)	ftr30	XP_003198033.2
Danio rerio (zebrafish)	btr22	XP_005173784.1
Danio rerio (zebrafish)	btr18	XP_005173787.1
Danio rerio (zebrafish)	btr32	XP_003201305.1
Danio rerio (zebrafish)	btr07	XP_001923237.4
Danio rerio (zebrafish)	LOC100001589	XP_001341559.3
Danio rerio (zebrafish)	ftr79	XP_697299.2
Danio rerio (zebrafish)	ftr83	XP_688583.3
Danio rerio (zebrafish)	LOC100333853	XP_002661845.3
Danio rerio (zebrafish)	si:dkeyp-123a12.2	XP_691204.4
Danio rerio (zebrafish)	LOC100007791	XP_001346158.5
Danio rerio (zebrafish)	si:ch211-250k18.8	XP_698776.5
Danio rerio (zebrafish)	zgc:174944	NP_001108057.1
Danio rerio (zebrafish)	LOC566957	XP_005174040.1
Danio rerio (zebrafish)	LOC100150225	XP_001921257.4
Danio rerio (zebrafish)	LOC100334101	XP_003197833.2
Danio rerio (zebrafish)	LOC100334843	XP_002665168.2
Danio rerio (zebrafish)	si:ch73-24k9.2	XP_002665507.3
Danio rerio (zebrafish)	si:ch211-196h16.5	XP_690098.5
Danio rerio (zebrafish)	si:ch211-194e1.7	XP_005167387.1
Danio rerio (zebrafish)	LOC793291	XP_001331884.5
Danio rerio (zebrafish)	LOC100004711	XP_001340175.3
Danio rerio (zebrafish)	LOC100534746	XP_005161332.1
Danio rerio (zebrafish)	LOC100005625	XP_001339269.4
Danio rerio (zebrafish)	LOC100003765	XP_001343238.2
Danio rerio (zebrafish)	LOC101883156	XP_005160435.1
Danio rerio (zebrafish)	si:ch211-237a4.2	XP_003198575.2
Danio rerio (zebrafish)	zgc:194906	NP_001124145.1
Danio rerio (zebrafish)	LOC100536980	XP_003201699.2
Danio rerio (zebrafish)	si:ch211-15j1.3	XP_002660683.2
Danio rerio (zebrafish)	si:ch211-233g6.6	XP_002660707.3
Danio rerio (zebrafish)	LOC100331097	XP_002661460.3
Danio rerio (zebrafish)	LOC101885873	XP_005161560.1
Danio rerio (zebrafish)	LOC101883187	XP_005174457.1
Danio rerio (zebrafish)	LOC101882512	XP_005171750.1
Danio rerio (zebrafish)	LOC100329306	XP_005168148.1
Danio rerio (zebrafish)	LOC100535915	XP_005161831.1
Danio rerio (zebrafish)	si:ch211-114l13.1	XP_002660672.3
Danio rerio (zebrafish)	LOC101885187	XP_005165034.1
Danio rerio (zebrafish)	si:rp71-7l19.2	XP_003198343.2
Danio rerio (zebrafish)	LOC100331680	XP_002667178.3
Danio rerio (zebrafish)	LOC100005516	XP_005171758.1
Danio rerio (zebrafish)	LOC101883084	XP_005174455.1
Danio rerio (zebrafish)	LOC101883426	XP_005174069.1
Danio rerio (zebrafish)	LOC100150280	XP_005174567.1
Danio rerio (zebrafish)	LOC101884637	XP_005168127.1
Danio rerio (zebrafish)	LOC100329543	XP_005167374.1
Danio rerio (zebrafish)	LOC101882306	XP_005171748.1
Danio rerio (zebrafish)	LOC566508	XP_694871.6
Danio rerio (zebrafish)	LOC101884187	XP_005157862.1
Danio rerio (zebrafish)	ftr55	XP_003200609.2
Danio rerio (zebrafish)	LOC100330813	XP_002660428.2
Danio rerio (zebrafish)	ftr49	XP_003198077.1
Danio rerio (zebrafish)	LOC100535633	XP_003198959.1
Danio rerio (zebrafish)	btr04	XP_003198956.2
Danio rerio (zebrafish)	btr09	NP_001124100.1
Danio rerio (zebrafish)	LOC558675	XP_687012.2
Danio rerio (zebrafish)	ftr76	XP_005157864.1
Danio rerio (zebrafish)	ftr16	XP_690796.5
Danio rerio (zebrafish)	LOC561829	XP_690320.2
Danio rerio (zebrafish)	ftr03	XP_002660930.2
Danio rerio (zebrafish)	ftr06	XP_001337856.1
Danio rerio (zebrafish)	LOC570185	XP_698724.1
Danio rerio (zebrafish)	LOC797531	XP_001338000.2
Danio rerio (zebrafish)	ftr02	XP_003197969.1
Danio rerio (zebrafish)	LOC571442	XP_700123.5
Danio rerio (zebrafish)	ftr19	XP_002660960.3
Danio rerio (zebrafish)	LOC101882488	XP_005163611.1
Danio rerio (zebrafish)	ftr39p	XP_002661035.2
Danio rerio (zebrafish)	zgc:136302	XP_005170221.1
Danio rerio (zebrafish)	ftr58	XP_686163.5
Danio rerio (zebrafish)	LOC100537283	XP_003198007.2
Danio rerio (zebrafish)	ftr64	XP_002661885.1
Danio rerio (zebrafish)	btr31	XP_005170663.1
Danio rerio (zebrafish)	LOC100006950	XP_001345543.5
Danio rerio (zebrafish)	ftr28l	XP_005163705.1
Danio rerio (zebrafish)	LOC100333325	XP_002667878.3
Danio rerio (zebrafish)	si:ch211-121a2.3	XP_698105.5
Danio rerio (zebrafish)	LOC799802	XP_001336792.2
Danio rerio (zebrafish)	LOC555438	XP_683030.6
Danio rerio (zebrafish)	LOC568716	XP_003201051.2
Danio rerio (zebrafish)	LOC568664	XP_003201050.2
Danio rerio (zebrafish)	si:ch73-286h23.3	XP_001346239.5
Danio rerio (zebrafish)	LOC100006645	XP_001337665.3
Danio rerio (zebrafish)	wu:fb15h11	XP_697949.6
Danio rerio (zebrafish)	si:ch211-191a16.2	XP_001338550.5
Danio rerio (zebrafish)	si:ch211-181d7.1	XP_001919795.3
Danio rerio (zebrafish)	LOC795182	XP_001332957.4
Danio rerio (zebrafish)	LOC100329836	XP_002664746.3
Danio rerio (zebrafish)	LOC100334547	XP_002664742.2
Danio rerio (zebrafish)	si:dkey-156m2.3	XP_002664795.3
Danio rerio (zebrafish)	si:ch211-213a13.6	XP_697815.6
Danio rerio (zebrafish)	si:dkey-13n15.11	XP_005165261.1
Danio rerio (zebrafish)	si:zfos-364h11.1	XP_002665163.2
Danio rerio (zebrafish)	LOC100002266	XP_005158672.1
Danio rerio (zebrafish)	si:ch211-278p9.3	XP_002665167.3
Danio rerio (zebrafish)	zgc:136791	XP_005160238.1
Danio rerio (zebrafish)	LOC796214	XP_005161889.1
Danio rerio (zebrafish)	LOC100536939	XP_003200134.2
Danio rerio (zebrafish)	zgc:174178	NP_001107787.1
Danio rerio (zebrafish)	LOC101882503	XP_005168090.1
Danio rerio (zebrafish)	si:ch211-114l13.4	XP_002660681.1
Danio rerio (zebrafish)	si:ch211-15j1.5	XP_002660682.2
Danio rerio (zebrafish)	LOC100334715	XP_005171097.1
Danio rerio (zebrafish)	si:ch211-15j1.1	XP_001920778.3
Danio rerio (zebrafish)	si:dkey-222h21.8	XP_002660686.2
Danio rerio (zebrafish)	si:dkey-222h21.5	XP_002660689.1
Danio rerio (zebrafish)	LOC101882744	XP_005168242.1
Danio rerio (zebrafish)	LOC100329650	XP_002661801.3
Danio rerio (zebrafish)	si:ch211-255g12.8	XP_694303.5
Danio rerio (zebrafish)	LOC100331576	XP_002661628.3
Danio rerio (zebrafish)	LOC101883760	XP_005164630.1
Danio rerio (zebrafish)	LOC101886147	XP_005167368.1
Danio rerio (zebrafish)	LOC101886579	XP_005161565.1
Danio rerio (zebrafish)	LOC100333957	XP_005171081.1
Danio rerio (zebrafish)	LOC101884123	XP_005171093.1
Danio rerio (zebrafish)	LOC101884874	XP_005171101.1
Danio rerio (zebrafish)	LOC100333264	XP_002660697.3
Danio rerio (zebrafish)	LOC100330744	XP_002660427.3
Danio rerio (zebrafish)	ftr50	XP_005164403.1
Danio rerio (zebrafish)	LOC793898	XP_001920428.2
Danio rerio (zebrafish)	LOC791496	XP_003198035.2
Danio rerio (zebrafish)	LOC565787	XP_694149.5
Danio rerio (zebrafish)	LOC570079	XP_698604.5
Danio rerio (zebrafish)	LOC570163	XP_003197979.1
Danio rerio (zebrafish)	btr16	XP_005157512.1
Danio rerio (zebrafish)	LOC570251	XP_698802.4
Danio rerio (zebrafish)	ftr15	XP_700104.3
Danio rerio (zebrafish)	ftr26	XP_005163617.1
Danio rerio (zebrafish)	ftr65	XP_003198031.2
Danio rerio (zebrafish)	zgc:173666	XP_003198037.1
Danio rerio (zebrafish)	ftr61	XP_003201315.1
Danio rerio (zebrafish)	btr20	XP_003200561.1
Danio rerio (zebrafish)	zgc:173581	XP_005173811.1
Danio rerio (zebrafish)	btr06	NP_001120951.1
Danio rerio (zebrafish)	LOC101886307	XP_005163673.1
Danio rerio (zebrafish)	LOC101884427	XP_005159614.1
Danio rerio (zebrafish)	LOC100002745	XP_005164415.1
Danio rerio (zebrafish)	LOC101885734	XP_005161559.1
Danio rerio (zebrafish)	si:dkey-151g22.1	NP_001137518.1
Danio rerio (zebrafish)	si:dkey-121n8.4	XP_001335920.4
Danio rerio (zebrafish)	LOC100330073	XP_002661806.3
Danio rerio (zebrafish)	si:dkey-7i4.1	XP_005169721.1
Danio rerio (zebrafish)	LOC100001465	XP_005166707.1
Danio rerio (zebrafish)	LOC100005483	XP_001339130.3
Danio rerio (zebrafish)	LOC562679	XP_691146.5
Danio rerio (zebrafish)	LOC100333840	XP_005169858.1
Danio rerio (zebrafish)	LOC100536924	XP_003200567.2
Danio rerio (zebrafish)	LOC559196	XP_687605.5
Danio rerio (zebrafish)	LOC795109	XP_001335224.5
Danio rerio (zebrafish)	si:ch211-187g4.1	XP_698878.6
Danio rerio (zebrafish)	LOC100538217	XP_005169783.1
Danio rerio (zebrafish)	LOC571651	XP_700351.5
Danio rerio (zebrafish)	si:ch211-271g18.1	XP_002661463.3
Danio rerio (zebrafish)	LOC565381	XP_693750.6
Danio rerio (zebrafish)	si:ch211-238e22.7	XP_005168160.1
Danio rerio (zebrafish)	LOC100333747	XP_002661530.3
Danio rerio (zebrafish)	LOC100331072	XP_002661905.3
Danio rerio (zebrafish)	si:ch73-236c18.7	XP_002660699.3
Danio rerio (zebrafish)	si:dkey-222h21.6	XP_002660688.2
Danio rerio (zebrafish)	si:ch211-233g6.3	XP_002660709.3
Danio rerio (zebrafish)	LOC100329354	XP_002661501.3
Danio rerio (zebrafish)	LOC100006280	XP_001920132.4
Danio rerio (zebrafish)	LOC101886178	XP_005164994.1
Danio rerio (zebrafish)	LOC101883660	XP_005168155.1
Danio rerio (zebrafish)	LOC101883625	XP_005170693.1
Danio rerio (zebrafish)	LOC100150791	XP_005165031.1
Danio rerio (zebrafish)	LOC101883234	XP_005160282.1
Danio rerio (zebrafish)	LOC101885938	XP_005173831.1
Danio rerio (zebrafish)	LOC101885252	XP_005168541.1
Danio rerio (zebrafish)	trpm2	XP_003198602.2
Danio rerio (zebrafish)	btr12	NP_001070087.1
Danio rerio (zebrafish)	btr02	NP_001070626.2
Danio rerio (zebrafish)	ftr72	NP_001007388.2
Danio rerio (zebrafish)	LOC101883527	XP_005170222.1
Danio rerio (zebrafish)	trim25l	XP_005172141.1
Danio rerio (zebrafish)	LOC100149991	XP_003200554.1
Danio rerio (zebrafish)	LOC101883221	XP_005171452.1
Danio rerio (zebrafish)	ftr56	XP_003200605.1
Danio rerio (zebrafish)	LOC100334710	XP_002665516.2
Danio rerio (zebrafish)	btr25	XP_005173808.1
Danio rerio (zebrafish)	ftr24	XP_003197988.1
Danio rerio (zebrafish)	LOC100536343	XP_003197990.1
Danio rerio (zebrafish)	LOC100537128	XP_003200077.2
Danio rerio (zebrafish)	LOC100536410	XP_003200565.2
Danio rerio (zebrafish)	btr21	XP_003200564.2
Danio rerio (zebrafish)	ftr84	XP_005165427.1
Danio rerio (zebrafish)	si:ch211-150h8.3	XP_003198032.1
Danio rerio (zebrafish)	ftr43	XP_005164401.1
Danio rerio (zebrafish)	LOC571488	XP_700178.4
Danio rerio (zebrafish)	ftr41	XP_695494.5
Danio rerio (zebrafish)	ftr59	XP_686796.2
Danio rerio (zebrafish)	ftr51	XP_691169.5
Danio rerio (zebrafish)	ftr14l	XP_003199766.1
Danio rerio (zebrafish)	si:ch211-214b16.3	XP_005164324.1
Danio rerio (zebrafish)	LOC100331532	XP_003201602.2
Danio rerio (zebrafish)	LOC100329480	XP_002663890.3
Danio rerio (zebrafish)	si:dkey-222h21.2	XP_001334961.4
Danio rerio (zebrafish)	LOC100006564	NP_001119957.1
Danio rerio (zebrafish)	LOC100333746	XP_002661485.3
Danio rerio (zebrafish)	LOC100329448	XP_002667651.3
Danio rerio (zebrafish)	si:ch211-114l13.10	XP_696367.5
Danio rerio (zebrafish)	LOC100331159	XP_005169797.1
Danio rerio (zebrafish)	si:ch211-213a13.9	XP_003201049.2
Danio rerio (zebrafish)	si:dkey-253d23.10	XP_683789.6
Danio rerio (zebrafish)	si:ch211-278p9.4	XP_001336197.5
Danio rerio (zebrafish)	LOC566782	XP_695160.6
Danio rerio (zebrafish)	LOC558818	NP_001038341.1
Danio rerio (zebrafish)	si:ch211-149a19.3	XP_005170041.1
Danio rerio (zebrafish)	si:dkey-222h21.9	XP_002660685.1
Danio rerio (zebrafish)	LOC796962	XP_001923433.2
Danio rerio (zebrafish)	LOC100536895	XP_003200133.2
Danio rerio (zebrafish)	LOC100537076	XP_003200135.2
Danio rerio (zebrafish)	LOC100329927	XP_003200136.2
Danio rerio (zebrafish)	si:ch211-233m11.1	XP_687146.4
Danio rerio (zebrafish)	LOC100535360	XP_003200157.2
Danio rerio (zebrafish)	LOC101884992	XP_005174036.1
Danio rerio (zebrafish)	LOC794214	XP_001334125.1
Danio rerio (zebrafish)	LOC100537399	XP_003198502.2
Danio rerio (zebrafish)	LOC100007745	XP_001346125.5
Danio rerio (zebrafish)	si:dkey-126g1.7	XP_005162400.1
Danio rerio (zebrafish)	LOC101882643	XP_005171752.1
Danio rerio (zebrafish)	LOC100001765	XP_001341704.4
Danio rerio (zebrafish)	si:dkey-82i20.1	XP_002666374.3
Danio rerio (zebrafish)	LOC100333748	XP_002661966.3
Danio rerio (zebrafish)	LOC565296	XP_003198495.2
Danio rerio (zebrafish)	LOC100535428	XP_005174493.1
Danio rerio (zebrafish)	LOC100334677	XP_002661542.3
Danio rerio (zebrafish)	LOC100329558	XP_002661549.3
Danio rerio (zebrafish)	si:dkeyp-4c4.1	NP_001094426.1
Danio rerio (zebrafish)	LOC101885760	XP_005164976.1
Danio rerio (zebrafish)	LOC101884884	XP_005157350.1
Danio rerio (zebrafish)	LOC101885212	XP_005158663.1
Danio rerio (zebrafish)	LOC101885461	XP_005168195.1
Danio rerio (zebrafish)	LOC560232	NP_001116726.1
Danio rerio (zebrafish)	LOC101885169	XP_005174039.1
Danio rerio (zebrafish)	LOC101883611	XP_005174070.1

Gene Ontology

The following LOC568664 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC568664 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa146925	XM_021471020.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa146926	XM_021471021.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa146927	XM_021471023.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa146927	XM_021471022.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa146928	XM_021471024.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa146929	XM_021471025.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa146930	XM_021471026.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa146931	XM_021471028.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa146932	XM_021471029.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa146933	XM_021471030.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa146934	XM_021471031.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa146935	XM_021471032.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa146936	XM_021471033.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa146937	XM_021471034.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa146938	XM_021471035.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa146939	XM_021471036.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa146940	XM_021471037.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa146941	XM_021471038.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa146942	XM_021471039.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa45489	XM_009298420.2 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa45490	XM_009298421.2 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa45491	XM_009298422.2 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa45492	XM_009298423.2 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa45493	XM_009298424.2 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa45494	XM_009298425.2 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa45495	XM_009298426.2 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa45496	XM_009298427.2 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa45497	XM_009298428.2 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa45498	XM_009298429.2 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa45501	XM_009298432.2 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa45502	XM_009298433.2 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa45505	XM_009298436.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa53641	XM_017354578.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa53642	XM_017354579.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa53643	XM_017354580.1 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC568664 ( XM_021471020.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326695.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X1, mRNA.

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CloneID	ODa146925
Clone ID Related Accession (Same CDS sequence)	XM_021471020.1
Accession Version	XM_021471020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG 
ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA 
CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT 
GAAGCCTGTA CACATCTGAA TAAAGTTCTG GGTGGAAACC TGTTACTCTT GAGAGAGCTG 
AATCTGAGTA AACTTGATCT TGGACCATCA GGATTGGAGA AACTTGCTGC TGTACTGAAA 
GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCAGTATGTC AGAAGAAGAT 
TGTCGTGTTT TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGACAGA GCTGAATTTG 
AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CCACTCTGTT TCAAAACCAA 
CAGTGTAGAC TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT 
GCTTTGGCTT CAGCATTTAA CTCAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGAG 
AATAAACTAG GAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCGCAGTGC 
ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGGATCCACA CTGTACACTG 
AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGACGCCT GTCGGTTTGT GACTAAAATT 
GTGGGTAAAA ACCCATTACT CCTGACAGAA CTGAATCTGA GTGATCGTAA ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGTAGAGT CAACACAATA 
ATGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTAACTTC AGCATTTATT 
TTAAACCCAT CACACCTGAT AGAGCTCAAT CTGAGTGGGA ATAAACTAGG AGACTCAGGA 
GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT ACACAATGCC GATTAGAGAA ACTGAGTCTT 
TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
AAGCTCACTG ATTTACTGCA CAGTAGAAGC TGTAAATTCA GATTTCTGAA GAGTTCTGCT 
GCACAGGAAG CATGTGACTA TCTTACTAAA GTTCTGGGTA TAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGCAA AGTGAAGAAA ATCAAGCTGA ATAATTGTGA GCTGACAGAG 
AAAAGCTGTT CACTTCTAGC TGCAGTTCTC TCCTCCAAAA CCATCCTGAA AGAGATGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCAGAGAAA TCTGTGAGGG ACTGAAGAAT 
CCTGTGTGTG AGCTGAAAAT ACTCAGTCTT TCAGACTGCC GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ACTTACTGGA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCACTTTGTG 
ACTGAAATGG TGGGTAAAAA CCCATTACTC CTGAAAGAAC TGAATCTGAG TGATCGTGTA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTGCTGC AGGATAAACA CTGTCAGATC 
CACACACTCA TTCTGTGTTA TTGCAGTATT ACAGAGGAAC AGTGTGTGAT CCTGACTTCA 
GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAGTGGAAA CAATCTAGGA 
GACTCTGCAG TGAAAAAACT CAGTGATTTA CTGATGAACC AACAATTCAA GCTGGAGAAA 
CTACGTCTGA GTGATTGCAG TATTACAGAG AAACAGTGTG TGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAATG GGAATAGACT AGGAGACTCT 
GGAGCTTATG GACTTGGCCA TCTGCTGAGC AGTGTGGATT GCAAGCTGAA TAAACTACAT 
CTGTGTGATT GCAGTATTAC AGGGAAACAG TTGCACTTCC TGATTGCAGT TATGTGTATG 
GACCCATCAC ACCTGAGGGT ACTGGACCTC AGTAAGAATC AAATAAAAAG CAGAGGAATG 
AACATGCTGT GTGATGTGCT GAATAAACCA CGCTGTAAAC TCAAGAGTTT AAGACTAAAT 
GACTGTGGCA TTACAGATGT TACACACTTA ACTCAGTCTT TGGCTGAGAA CAAAGCACTG 
GAGTTCTTAA AAGAGCTTGA ACTGAGTAAA AATCAAATGG GAGACTCCGA AAAGCAAAAA 
CTCAGTGACC TGCTGCGAGA CTCAAACTGT AAACTGAGGT TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326695.1
CDS	182..7489
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471020.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471020.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG 
ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA 
CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT 
GAAGCCTGTA CACATCTGAA TAAAGTTCTG GGTGGAAACC TGTTACTCTT GAGAGAGCTG 
AATCTGAGTA AACTTGATCT TGGACCATCA GGATTGGAGA AACTTGCTGC TGTACTGAAA 
GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCAGTATGTC AGAAGAAGAT 
TGTCGTGTTT TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGACAGA GCTGAATTTG 
AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CCACTCTGTT TCAAAACCAA 
CAGTGTAGAC TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT 
GCTTTGGCTT CAGCATTTAA CTCAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGAG 
AATAAACTAG GAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCGCAGTGC 
ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGGATCCACA CTGTACACTG 
AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGACGCCT GTCGGTTTGT GACTAAAATT 
GTGGGTAAAA ACCCATTACT CCTGACAGAA CTGAATCTGA GTGATCGTAA ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTACTG CAGGATAAAC ACTGTAGAGT CAACACAATA 
ATGCTGAATA ATAACAGGAT TACAGCAGAA GGTTGTGCTG CTTTAACTTC AGCATTTATT 
TTAAACCCAT CACACCTGAT AGAGCTCAAT CTGAGTGGGA ATAAACTAGG AGACTCAGGA 
GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT ACACAATGCC GATTAGAGAA ACTGAGTCTT 
TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
AAGCTCACTG ATTTACTGCA CAGTAGAAGC TGTAAATTCA GATTTCTGAA GAGTTCTGCT 
GCACAGGAAG CATGTGACTA TCTTACTAAA GTTCTGGGTA TAAGTCCATT ATTACTGACA 
GAACTGGATC TGAGTGAAGA TAAATTAGGA GATCTGGATG GAGAGAAACT CTCTGCTCTT 
CTGATGGACT CTCATAGCAA AGTGAAGAAA ATCAAGCTGA ATAATTGTGA GCTGACAGAG 
AAAAGCTGTT CACTTCTAGC TGCAGTTCTC TCCTCCAAAA CCATCCTGAA AGAGATGAAC 
CTGAACAACA GTCGTCTGCT GGACTCAGGA GTCAGAGAAA TCTGTGAGGG ACTGAAGAAT 
CCTGTGTGTG AGCTGAAAAT ACTCAGTCTT TCAGACTGCC GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ACTTACTGGA GGATCCACAC 
TGTACACTGA AGACACTGAG GTTTTTGAGT CCTGCTGCTG ATGAAGGCTG TCACTTTGTG 
ACTGAAATGG TGGGTAAAAA CCCATTACTC CTGAAAGAAC TGAATCTGAG TGATCGTGTA 
CTAGGAGACA CACGAGTGAA TCAACTCTCT GCTCTGCTGC AGGATAAACA CTGTCAGATC 
CACACACTCA TTCTGTGTTA TTGCAGTATT ACAGAGGAAC AGTGTGTGAT CCTGACTTCA 
GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAGTGGAAA CAATCTAGGA 
GACTCTGCAG TGAAAAAACT CAGTGATTTA CTGATGAACC AACAATTCAA GCTGGAGAAA 
CTACGTCTGA GTGATTGCAG TATTACAGAG AAACAGTGTG TGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAATG GGAATAGACT AGGAGACTCT 
GGAGCTTATG GACTTGGCCA TCTGCTGAGC AGTGTGGATT GCAAGCTGAA TAAACTACAT 
CTGTGTGATT GCAGTATTAC AGGGAAACAG TTGCACTTCC TGATTGCAGT TATGTGTATG 
GACCCATCAC ACCTGAGGGT ACTGGACCTC AGTAAGAATC AAATAAAAAG CAGAGGAATG 
AACATGCTGT GTGATGTGCT GAATAAACCA CGCTGTAAAC TCAAGAGTTT AAGACTAAAT 
GACTGTGGCA TTACAGATGT TACACACTTA ACTCAGTCTT TGGCTGAGAA CAAAGCACTG 
GAGTTCTTAA AAGAGCTTGA ACTGAGTAAA AATCAAATGG GAGACTCCGA AAAGCAAAAA 
CTCAGTGACC TGCTGCGAGA CTCAAACTGT AAACTGAGGT TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471021.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326696.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X3, mRNA.

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CloneID	ODa146926
Clone ID Related Accession (Same CDS sequence)	XM_021471021.1
Accession Version	XM_021471021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAA ACTTTCGATT AACTTACATT TTAGAAAACA TTGAAGGATC TCAGGTGTGT 
TTGAGCTGTG ACTTAGTCTT TTTAAAGGTT TCTGGATTCT GTTTCTGCTG CACAAGACCG 
TCTTCTCGAC ATGTAAGATC AGGTTCGTAT GTATCCAGCT CTGTGTCTGT GAAGAGTGAT 
GATTCAAAAA AAGGAGGTCA GCCAAACTTC AGTAGAGAAA CACTGTCACC TACCACAAGT 
GTTCAAAATG AGTCTTCAGA CACAGACATC CAGACTCAGA AAAAACACAT GAGTTTTAAA 
GACAATCTTC AGGGGATCTT CCAGGATCTT GAGAACCAAA TATTAAAATT TCTGAAGAAT 
GAGCTAGAAA AGTTTAAGAA AATATTACAT GAAGAAAATA CAGAATACTT TGTAAAGGAC 
TTTGGTGAGA ACAGATGCAG AATAAAAGAA GCAGCTCTTG ATCTCACACT CTACTTCCTG 
AAAGAAATGA AGCAAGATGA AGCAGCAGAT ACTCTAGAAG ATGAGCTGTT CTTCATTCAT 
CAGCTGAAAT GTGGCCTACA GAAGAAGTAT CAGTGTGTGT TTGAAGGAAT TGCACAGCAA 
GGAGACTCCA CACTCCTGAA GAACATCTAC ACAGATCTCT ATATCACTCA GGGTGGCAGT 
GAACAGGTCA ATACTGAACA TGAGGTCAGG CAGATTGAAG TTACTTCCAG ACGTCATAAA 
GCTCAAGAGA TACAGGTTGA ATGCAGACAT TTGTTTGAAG CGTCTGAACA AGACAAGCAG 
ATCAGAACTG TACTGACAAA AGGAGTCGCT GGCATCGGGA AATCAGTCTC TGTGCAAAAG 
TTTGTTCTGG ATTGGGCTGA AGGAAAAGAA AATCAAGAGA TCAGCTTCAT ATTTCCTCTT 
CCATTCAGAG AGATGAACTT AAAGGAGAAA GAAACACCCA GTTTGATGGA GCTTATAACT 
CAGTTTTACC CAGAGACAAA AGGACTGAAC CTTACAAGAA GGAATCGATT CAAAGTCCTG 
TTCATCCTTG ATGGATTGGA TGAATGTCGT CTTCCTCTGA ACTTTGCTGG TAATGAGACG 
TGTCGTGATG TTTCGTCACC AGCCTCTCTG GATGTTCTCC TGACGAACCT CATCAAGGGA 
AATCTGCTTC CTTCTGCTCT CATCTGGATC ACCAGCAGAC CAGCAGCTGC CAGTAAGATT 
CCTCCTGACT GTATCGACAG AGTGACAGAG ATACGAGGAT TCAATGATGC ACAAAAGGAG 
GAGTACTTCA GAAAAAGACT CACAGATGAG AGTCTGGCCA AAGAAATCAT TGATCATGTT 
AAAAAATCAA AGAGTCTTTT CATCATGTGC CACATCCCAG TCTTCTGCTG GATTTCAGCC 
ACTGTTCTCC AGAACATTTT ACAGAAGAAG AGAAAAAATG TGAAAAACAA TCAGGCTGAT 
GACGCCTCCA AAACACTGCA GGAATCAAAC ACTGAAGACA CTCCCAAGAC TCTGACACAA 
ATGTACACAC ACTTCTTGCA CGTTCAGATC AAGCAGAGCA GACAAAAGTA TGATGGAGAA 
AACAAACCAG ATGTGTCCTG GGATAAAGAC GCCATCCTTT CACTGGGGAA ACTGGCATTT 
CAGCAGCTGC TAAACAACAA CCTGATCTTC TATGAAACAG ACCTGGAAGC CTGTGGTATT 
GACGTCTATA AAGCTGAAAA GTTCTCAGGC ATGTGTACTG AGATCTTTAA GGAGGAAACA 
GGGATTGTTC TTGGTACCAT GTACTGCTTT GTTCACTTGA GTGTTCAAGA GTTTATTGCA 
GCCCTTTATG CACATCTGAT TCTGAACATC AACAATAGAA GTGTGTTTGA TCAAAACACA 
AGAAATAGAA ATGTGTCCAT GATTGATTTG CTGAAGACTG CAGTGGACAA GGCACTTGAG 
AGTGACAATG GACACCTGGA CCTTTTCCTT CGCTTCCTCC TTGGTCTGTC ACTCCAGTCC 
AACAGTGGAA TCTTAAAAGG CCTGTTGACA CAGCAAGACC ACAATAACTG GAGCAAATGG 
AAAATACTTC AGTACTTCTT TAGAACAGTA GATCAGCAGC AAGACCACAA TGACCAGATC 
AAAGAGGAAA TAGTTCATTA CATCAAGCAG AAGTTTGAAG CAAATCTTTC TCTTGAGAGA 
TCCATCAACC TGTTCCACTG TCTGAATGAA CTAAACGATC AAACTCTGGT GAAAGAGATT 
CAGTCTCACC TTAGCAAAGG AAGGTACTCA TCTTCTGACC TTTCACCTGC CCAGTGGTCT 
GCTCTGGTCT TTGTGTTGTT GACATCAGAT GAAGAGATGG AGGAGTTTGA GCTTCAGAAA 
TTCAAGAAAT CAGACGAGTG TCTCATCAGG CTATTAGCTG TCATCAAAAC CTGCAAAAGA 
GCACTGCTCA GTAAGTGTAA TCTAACAGAG GAAAGCTGTT CAGCTCTGGC TTCAGTTCTC 
AGATCAGACT CCAGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGGATTCA 
GGAGTGAAGC TGCTCTCTGA TGGACTGAAG GACAGTAACT GTACACTGGA GAAACTCAGA 
CTTTCAGAGT GCGGTATCAA TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACC ATCAGGAGTG 
AAGGAGCTCA CTGATTTACT GAAACCAGAC TATACACTTA AAACACTGAG GTTTTTGAGT 
CCTGCTGCAG AAGAAGTCTT TCAGTTTGTG ACTAAAATTG TGGGTAAAAA CCCGTTACTC 
CTGAAAGAAC TGAATCTGAG TGATCGTGAA CTAGGAGATA CACGAGTGAA TCAACTCTCT 
GCTCTGCTGC AGGATAAACA CTGTAGACTC AACACACTCA TGCTGAATAA TAACAGGATT 
ACAGCAGAAG GTTGTGCTGC TTTGACTTTT GCATTTAATT TAAATCCTTC ACACCTGATA 
GAGCTCAATC TGAGTGGGAA TAAACTAGGA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG 
CTGGAAAATA CACAATGCCA ATTAGAGAAA CTGAGGCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA TTTACAGCAG 
GATCCACACT GTACACTGAA GATACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGCCTGT 
CAGTTTTTGA CTGGAGTTGT GGGTAAAAAC CTGTTACTCT TGAAAGAGCT GAATCTGAGT 
GGACATGAAC TAGGACTCTC AGACAGCTTT CAAATGCTTG TTCTTTTACT GCATGATAAA 
CACTGTACGC TCAACACACT GATTCTTTCA GATTGCAGTA TCACAGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAC ATCAAACCCA TCATACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAA CTCACTGATT TACTACTGGA TGAACGCTGT 
AGCTTAAAAA TCATCAGGTT TCTGAAAAGT CCTGCTGCAC AAGAGTCATG TGATTTTCTC 
ACTGAAGTTC TGGATGTAAA TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGATAAA 
TTAGGAGATC TGGATGGAGA GAAAATCTCT GCTTTTTTGA TGGACCCTCA TAGCAAAGTG 
GAGAAAATCA AGCTGAATAA TTGTAAGCTG CTTGAGAAAA GCTCATCAGT TTTAGCAACA 
GTTCTGTCTT CCAAAACCAT CCTGAAAGAG CTGAACCTGA ACAACAGTCG TCTGCTGGAC 
TCAGGAGTCA GAGAGATCTG TGAGGGACTG AAGAATCCTG TGTGTGAGCT GAAGATACTC 
AAGCTTTCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA AGGCTGTCAG TTTGTGACTG GAATTGTGGG TAAAAACCCA 
TTACTTCTGA AAGAGCTAAA TCTGAGTGAT CGTGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT AAACTCAACA CACTCATTCT TAGTAAATGT 
GGTGTAACAG TGGAAAGCTG TTCAGCTCTG GCTACAGTCC TGAGATCAGA GACCAGTCTA 
AAAGAGCTTG ACATGAGCAA CAATAATCTG CAGGAATCAG GAGTGAAGAA ACTCCAGAGC 
GGATTAAAAA ACACAAACTG CACACTGGAA AAACTCAGTC TTTTAGACTG CAGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGAAGCTCAC TGAGTTACTG 
AAACCAGACT ATACACTGAA GACACTGAGG TTTTTGAGTG ATGATGCAGA TGAAGCCTGT 
ACACATCTGA ATAAAGTTCT GGGTGGAAAC CTGTTACTCT TGAGAGAGCT GAATCTGAGT 
AAACTTGATC TTGGACCATC AGGATTGGAG AAACTTGCTG CTGTACTGAA AGATAAACAC 
TGTAAACTCA ACACACTGAT TCTGAATAAC AGCAGTATGT CAGAAGAAGA TTGTCGTGTT 
TTGGCTGAAG CTTTTAATTC AAACCCATCA AATCTGACAG AGCTGAATTT GAGTGGGACT 
AAACTCACAA ACTCAGGAAT GAAGAGCTTC ACCACTCTGT TTCAAAACCA ACAGTGTAGA 
CTGGAAAAGC TGAAGTTCAG CAGCATGTGT ATTACAGCAG AAGGTTGTGC TGCTTTGGCT 
TCAGCATTTA ACTCAAATCC TTCACACCTG ATAGAGCTCA ATCTGAGTGA GAATAAACTA 
GGAGACTCAG GAGTAACAGA AATCTCCAGT CTACTGGCAA ACTCGCAGTG CATGCTGAAG 
ATACTCGGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGACGCC TGTCGGTTTG TGACTAAAAT TGTGGGTAAA 
AACCCATTAC TCCTGACAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAGAG TCAACACAAT AATGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAT TTTAAACCCA 
TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAGAGAAG 
ATCTGTCCTC TGCTGGAAAA TACACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG 
AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG 
GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC 
ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA 
GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT 
GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA 
CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG 
TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326696.1
CDS	388..7626
Translation	MSQNFRLTYILENIEGSQVCLSCDLVFLKVSGFCFCCTRPSSRHVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471021.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471021.1

ORF Insert Sequence:

ATGAGCCAAA ACTTTCGATT AACTTACATT TTAGAAAACA TTGAAGGATC TCAGGTGTGT 
TTGAGCTGTG ACTTAGTCTT TTTAAAGGTT TCTGGATTCT GTTTCTGCTG CACAAGACCG 
TCTTCTCGAC ATGTAAGATC AGGTTCGTAT GTATCCAGCT CTGTGTCTGT GAAGAGTGAT 
GATTCAAAAA AAGGAGGTCA GCCAAACTTC AGTAGAGAAA CACTGTCACC TACCACAAGT 
GTTCAAAATG AGTCTTCAGA CACAGACATC CAGACTCAGA AAAAACACAT GAGTTTTAAA 
GACAATCTTC AGGGGATCTT CCAGGATCTT GAGAACCAAA TATTAAAATT TCTGAAGAAT 
GAGCTAGAAA AGTTTAAGAA AATATTACAT GAAGAAAATA CAGAATACTT TGTAAAGGAC 
TTTGGTGAGA ACAGATGCAG AATAAAAGAA GCAGCTCTTG ATCTCACACT CTACTTCCTG 
AAAGAAATGA AGCAAGATGA AGCAGCAGAT ACTCTAGAAG ATGAGCTGTT CTTCATTCAT 
CAGCTGAAAT GTGGCCTACA GAAGAAGTAT CAGTGTGTGT TTGAAGGAAT TGCACAGCAA 
GGAGACTCCA CACTCCTGAA GAACATCTAC ACAGATCTCT ATATCACTCA GGGTGGCAGT 
GAACAGGTCA ATACTGAACA TGAGGTCAGG CAGATTGAAG TTACTTCCAG ACGTCATAAA 
GCTCAAGAGA TACAGGTTGA ATGCAGACAT TTGTTTGAAG CGTCTGAACA AGACAAGCAG 
ATCAGAACTG TACTGACAAA AGGAGTCGCT GGCATCGGGA AATCAGTCTC TGTGCAAAAG 
TTTGTTCTGG ATTGGGCTGA AGGAAAAGAA AATCAAGAGA TCAGCTTCAT ATTTCCTCTT 
CCATTCAGAG AGATGAACTT AAAGGAGAAA GAAACACCCA GTTTGATGGA GCTTATAACT 
CAGTTTTACC CAGAGACAAA AGGACTGAAC CTTACAAGAA GGAATCGATT CAAAGTCCTG 
TTCATCCTTG ATGGATTGGA TGAATGTCGT CTTCCTCTGA ACTTTGCTGG TAATGAGACG 
TGTCGTGATG TTTCGTCACC AGCCTCTCTG GATGTTCTCC TGACGAACCT CATCAAGGGA 
AATCTGCTTC CTTCTGCTCT CATCTGGATC ACCAGCAGAC CAGCAGCTGC CAGTAAGATT 
CCTCCTGACT GTATCGACAG AGTGACAGAG ATACGAGGAT TCAATGATGC ACAAAAGGAG 
GAGTACTTCA GAAAAAGACT CACAGATGAG AGTCTGGCCA AAGAAATCAT TGATCATGTT 
AAAAAATCAA AGAGTCTTTT CATCATGTGC CACATCCCAG TCTTCTGCTG GATTTCAGCC 
ACTGTTCTCC AGAACATTTT ACAGAAGAAG AGAAAAAATG TGAAAAACAA TCAGGCTGAT 
GACGCCTCCA AAACACTGCA GGAATCAAAC ACTGAAGACA CTCCCAAGAC TCTGACACAA 
ATGTACACAC ACTTCTTGCA CGTTCAGATC AAGCAGAGCA GACAAAAGTA TGATGGAGAA 
AACAAACCAG ATGTGTCCTG GGATAAAGAC GCCATCCTTT CACTGGGGAA ACTGGCATTT 
CAGCAGCTGC TAAACAACAA CCTGATCTTC TATGAAACAG ACCTGGAAGC CTGTGGTATT 
GACGTCTATA AAGCTGAAAA GTTCTCAGGC ATGTGTACTG AGATCTTTAA GGAGGAAACA 
GGGATTGTTC TTGGTACCAT GTACTGCTTT GTTCACTTGA GTGTTCAAGA GTTTATTGCA 
GCCCTTTATG CACATCTGAT TCTGAACATC AACAATAGAA GTGTGTTTGA TCAAAACACA 
AGAAATAGAA ATGTGTCCAT GATTGATTTG CTGAAGACTG CAGTGGACAA GGCACTTGAG 
AGTGACAATG GACACCTGGA CCTTTTCCTT CGCTTCCTCC TTGGTCTGTC ACTCCAGTCC 
AACAGTGGAA TCTTAAAAGG CCTGTTGACA CAGCAAGACC ACAATAACTG GAGCAAATGG 
AAAATACTTC AGTACTTCTT TAGAACAGTA GATCAGCAGC AAGACCACAA TGACCAGATC 
AAAGAGGAAA TAGTTCATTA CATCAAGCAG AAGTTTGAAG CAAATCTTTC TCTTGAGAGA 
TCCATCAACC TGTTCCACTG TCTGAATGAA CTAAACGATC AAACTCTGGT GAAAGAGATT 
CAGTCTCACC TTAGCAAAGG AAGGTACTCA TCTTCTGACC TTTCACCTGC CCAGTGGTCT 
GCTCTGGTCT TTGTGTTGTT GACATCAGAT GAAGAGATGG AGGAGTTTGA GCTTCAGAAA 
TTCAAGAAAT CAGACGAGTG TCTCATCAGG CTATTAGCTG TCATCAAAAC CTGCAAAAGA 
GCACTGCTCA GTAAGTGTAA TCTAACAGAG GAAAGCTGTT CAGCTCTGGC TTCAGTTCTC 
AGATCAGACT CCAGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGGATTCA 
GGAGTGAAGC TGCTCTCTGA TGGACTGAAG GACAGTAACT GTACACTGGA GAAACTCAGA 
CTTTCAGAGT GCGGTATCAA TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACC ATCAGGAGTG 
AAGGAGCTCA CTGATTTACT GAAACCAGAC TATACACTTA AAACACTGAG GTTTTTGAGT 
CCTGCTGCAG AAGAAGTCTT TCAGTTTGTG ACTAAAATTG TGGGTAAAAA CCCGTTACTC 
CTGAAAGAAC TGAATCTGAG TGATCGTGAA CTAGGAGATA CACGAGTGAA TCAACTCTCT 
GCTCTGCTGC AGGATAAACA CTGTAGACTC AACACACTCA TGCTGAATAA TAACAGGATT 
ACAGCAGAAG GTTGTGCTGC TTTGACTTTT GCATTTAATT TAAATCCTTC ACACCTGATA 
GAGCTCAATC TGAGTGGGAA TAAACTAGGA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG 
CTGGAAAATA CACAATGCCA ATTAGAGAAA CTGAGGCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA TTTACAGCAG 
GATCCACACT GTACACTGAA GATACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGCCTGT 
CAGTTTTTGA CTGGAGTTGT GGGTAAAAAC CTGTTACTCT TGAAAGAGCT GAATCTGAGT 
GGACATGAAC TAGGACTCTC AGACAGCTTT CAAATGCTTG TTCTTTTACT GCATGATAAA 
CACTGTACGC TCAACACACT GATTCTTTCA GATTGCAGTA TCACAGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAC ATCAAACCCA TCATACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAA CTCACTGATT TACTACTGGA TGAACGCTGT 
AGCTTAAAAA TCATCAGGTT TCTGAAAAGT CCTGCTGCAC AAGAGTCATG TGATTTTCTC 
ACTGAAGTTC TGGATGTAAA TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGATAAA 
TTAGGAGATC TGGATGGAGA GAAAATCTCT GCTTTTTTGA TGGACCCTCA TAGCAAAGTG 
GAGAAAATCA AGCTGAATAA TTGTAAGCTG CTTGAGAAAA GCTCATCAGT TTTAGCAACA 
GTTCTGTCTT CCAAAACCAT CCTGAAAGAG CTGAACCTGA ACAACAGTCG TCTGCTGGAC 
TCAGGAGTCA GAGAGATCTG TGAGGGACTG AAGAATCCTG TGTGTGAGCT GAAGATACTC 
AAGCTTTCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGAGTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA AGGCTGTCAG TTTGTGACTG GAATTGTGGG TAAAAACCCA 
TTACTTCTGA AAGAGCTAAA TCTGAGTGAT CGTGAACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TACTGCAGGA TAAACACTGT AAACTCAACA CACTCATTCT TAGTAAATGT 
GGTGTAACAG TGGAAAGCTG TTCAGCTCTG GCTACAGTCC TGAGATCAGA GACCAGTCTA 
AAAGAGCTTG ACATGAGCAA CAATAATCTG CAGGAATCAG GAGTGAAGAA ACTCCAGAGC 
GGATTAAAAA ACACAAACTG CACACTGGAA AAACTCAGTC TTTTAGACTG CAGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGAAGCTCAC TGAGTTACTG 
AAACCAGACT ATACACTGAA GACACTGAGG TTTTTGAGTG ATGATGCAGA TGAAGCCTGT 
ACACATCTGA ATAAAGTTCT GGGTGGAAAC CTGTTACTCT TGAGAGAGCT GAATCTGAGT 
AAACTTGATC TTGGACCATC AGGATTGGAG AAACTTGCTG CTGTACTGAA AGATAAACAC 
TGTAAACTCA ACACACTGAT TCTGAATAAC AGCAGTATGT CAGAAGAAGA TTGTCGTGTT 
TTGGCTGAAG CTTTTAATTC AAACCCATCA AATCTGACAG AGCTGAATTT GAGTGGGACT 
AAACTCACAA ACTCAGGAAT GAAGAGCTTC ACCACTCTGT TTCAAAACCA ACAGTGTAGA 
CTGGAAAAGC TGAAGTTCAG CAGCATGTGT ATTACAGCAG AAGGTTGTGC TGCTTTGGCT 
TCAGCATTTA ACTCAAATCC TTCACACCTG ATAGAGCTCA ATCTGAGTGA GAATAAACTA 
GGAGACTCAG GAGTAACAGA AATCTCCAGT CTACTGGCAA ACTCGCAGTG CATGCTGAAG 
ATACTCGGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGACGCC TGTCGGTTTG TGACTAAAAT TGTGGGTAAA 
AACCCATTAC TCCTGACAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAGAG TCAACACAAT AATGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAT TTTAAACCCA 
TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAGAGAAG 
ATCTGTCCTC TGCTGGAAAA TACACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG 
AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG 
GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC 
ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA 
GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT 
GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA 
CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG 
TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471022.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326697.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X4, mRNA.

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CloneID	ODa146927
Clone ID Related Accession (Same CDS sequence)	XM_021471022.1 , XM_021471023.1
Accession Version	XM_021471022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGG AGCGAGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAG ATCAGGTTCG 
TATGTATCCA GCTCTGTGTC TGTGAAGAGT GATGATTCAA AAAAAGGAGG TCAGCCAAAC 
TTCAGTAGAG AAACACTGTC ACCTACCACA AGTGTTCAAA ATGAGTCTTC AGACACAGAC 
ATCCAGACTC AGAAAAAACA CATGAGTTTT AAAGACAATC TTCAGGGGAT CTTCCAGGAT 
CTTGAGAACC AAATATTAAA ATTTCTGAAG AATGAGCTAG AAAAGTTTAA GAAAATATTA 
CATGAAGAAA ATACAGAATA CTTTGTAAAG GACTTTGGTG AGAACAGATG CAGAATAAAA 
GAAGCAGCTC TTGATCTCAC ACTCTACTTC CTGAAAGAAA TGAAGCAAGA TGAAGCAGCA 
GATACTCTAG AAGATGAGCT GTTCTTCATT CATCAGCTGA AATGTGGCCT ACAGAAGAAG 
TATCAGTGTG TGTTTGAAGG AATTGCACAG CAAGGAGACT CCACACTCCT GAAGAACATC 
TACACAGATC TCTATATCAC TCAGGGTGGC AGTGAACAGG TCAATACTGA ACATGAGGTC 
AGGCAGATTG AAGTTACTTC CAGACGTCAT AAAGCTCAAG AGATACAGGT TGAATGCAGA 
CATTTGTTTG AAGCGTCTGA ACAAGACAAG CAGATCAGAA CTGTACTGAC AAAAGGAGTC 
GCTGGCATCG GGAAATCAGT CTCTGTGCAA AAGTTTGTTC TGGATTGGGC TGAAGGAAAA 
GAAAATCAAG AGATCAGCTT CATATTTCCT CTTCCATTCA GAGAGATGAA CTTAAAGGAG 
AAAGAAACAC CCAGTTTGAT GGAGCTTATA ACTCAGTTTT ACCCAGAGAC AAAAGGACTG 
AACCTTACAA GAAGGAATCG ATTCAAAGTC CTGTTCATCC TTGATGGATT GGATGAATGT 
CGTCTTCCTC TGAACTTTGC TGGTAATGAG ACGTGTCGTG ATGTTTCGTC ACCAGCCTCT 
CTGGATGTTC TCCTGACGAA CCTCATCAAG GGAAATCTGC TTCCTTCTGC TCTCATCTGG 
ATCACCAGCA GACCAGCAGC TGCCAGTAAG ATTCCTCCTG ACTGTATCGA CAGAGTGACA 
GAGATACGAG GATTCAATGA TGCACAAAAG GAGGAGTACT TCAGAAAAAG ACTCACAGAT 
GAGAGTCTGG CCAAAGAAAT CATTGATCAT GTTAAAAAAT CAAAGAGTCT TTTCATCATG 
TGCCACATCC CAGTCTTCTG CTGGATTTCA GCCACTGTTC TCCAGAACAT TTTACAGAAG 
AAGAGAAAAA ATGTGAAAAA CAATCAGGCT GATGACGCCT CCAAAACACT GCAGGAATCA 
AACACTGAAG ACACTCCCAA GACTCTGACA CAAATGTACA CACACTTCTT GCACGTTCAG 
ATCAAGCAGA GCAGACAAAA GTATGATGGA GAAAACAAAC CAGATGTGTC CTGGGATAAA 
GACGCCATCC TTTCACTGGG GAAACTGGCA TTTCAGCAGC TGCTAAACAA CAACCTGATC 
TTCTATGAAA CAGACCTGGA AGCCTGTGGT ATTGACGTCT ATAAAGCTGA AAAGTTCTCA 
GGCATGTGTA CTGAGATCTT TAAGGAGGAA ACAGGGATTG TTCTTGGTAC CATGTACTGC 
TTTGTTCACT TGAGTGTTCA AGAGTTTATT GCAGCCCTTT ATGCACATCT GATTCTGAAC 
ATCAACAATA GAAGTGTGTT TGATCAAAAC ACAAGAAATA GAAATGTGTC CATGATTGAT 
TTGCTGAAGA CTGCAGTGGA CAAGGCACTT GAGAGTGACA ATGGACACCT GGACCTTTTC 
CTTCGCTTCC TCCTTGGTCT GTCACTCCAG TCCAACAGTG GAATCTTAAA AGGCCTGTTG 
ACACAGCAAG ACCACAATAA CTGGAGCAAA TGGAAAATAC TTCAGTACTT CTTTAGAACA 
GTAGATCAGC AGCAAGACCA CAATGACCAG ATCAAAGAGG AAATAGTTCA TTACATCAAG 
CAGAAGTTTG AAGCAAATCT TTCTCTTGAG AGATCCATCA ACCTGTTCCA CTGTCTGAAT 
GAACTAAACG ATCAAACTCT GGTGAAAGAG ATTCAGTCTC ACCTTAGCAA AGGAAGGTAC 
TCATCTTCTG ACCTTTCACC TGCCCAGTGG TCTGCTCTGG TCTTTGTGTT GTTGACATCA 
GATGAAGAGA TGGAGGAGTT TGAGCTTCAG AAATTCAAGA AATCAGACGA GTGTCTCATC 
AGGCTATTAG CTGTCATCAA AACCTGCAAA AGAGCACTGC TCAGTAAGTG TAATCTAACA 
GAGGAAAGCT GTTCAGCTCT GGCTTCAGTT CTCAGATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA ACTGTACACT GGAGAAACTC AGACTTTCAG AGTGCGGTAT CAATGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACCATCAGGA GTGAAGGAGC TCACTGATTT ACTGAAACCA 
GACTATACAC TTAAAACACT GAGGTTTTTG AGTCCTGCTG CAGAAGAAGT CTTTCAGTTT 
GTGACTAAAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT 
GAACTAGGAG ATACACGAGT GAATCAACTC TCTGCTCTGC TGCAGGATAA ACACTGTAGA 
CTCAACACAC TCATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTGACT 
TTTGCATTTA ATTTAAATCC TTCACACCTG ATAGAGCTCA ATCTGAGTGG GAATAAACTA 
GGAGACTCAG GAGTAGAGAA GATCTGTCCT CTGCTGGAAA ATACACAATG CCAATTAGAG 
AAACTGAGGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGATTTACAG CAGGATCCAC ACTGTACACT GAAGATACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGCC TGTCAGTTTT TGACTGGAGT TGTGGGTAAA 
AACCTGTTAC TCTTGAAAGA GCTGAATCTG AGTGGACATG AACTAGGACT CTCAGACAGC 
TTTCAAATGC TTGTTCTTTT ACTGCATGAT AAACACTGTA CGCTCAACAC ACTGATTCTT 
TCAGATTGCA GTATCACAGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GACATCAAAC 
CCATCATACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAACTCACTG ATTTACTACT GGATGAACGC TGTAGCTTAA AAATCATCAG GTTTCTGAAA 
AGTCCTGCTG CACAAGAGTC ATGTGATTTT CTCACTGAAG TTCTGGATGT AAATCCATTA 
TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAAATC 
TCTGCTTTTT TGATGGACCC TCATAGCAAA GTGGAGAAAA TCAAGCTGAA TAATTGTAAG 
CTGCTTGAGA AAAGCTCATC AGTTTTAGCA ACAGTTCTGT CTTCCAAAAC CATCCTGAAA 
GAGCTGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAGAT CTGTGAGGGA 
CTGAAGAATC CTGTGTGTGA GCTGAAGATA CTCAAGCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA GTTACTGCAG 
GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCATTACTTC TGAAAGAGCT AAATCTGAGT 
GATCGTGAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGTAAACTCA ACACACTCAT TCTTAGTAAA TGTGGTGTAA CAGTGGAAAG CTGTTCAGCT 
CTGGCTACAG TCCTGAGATC AGAGACCAGT CTAAAAGAGC TTGACATGAG CAACAATAAT 
CTGCAGGAAT CAGGAGTGAA GAAACTCCAG AGCGGATTAA AAAACACAAA CTGCACACTG 
GAAAAACTCA GTCTTTTAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGAAGCT CACTGAGTTA CTGAAACCAG ACTATACACT GAAGACACTG 
AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA 
AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG 
GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT 
AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA 
TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC 
TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG 
TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC 
AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC 
GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT 
CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT 
GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA 
TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA 
TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG 
GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG 
CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC 
AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA 
GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC 
TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT 
GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA 
GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG 
CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG 
GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG 
AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG 
GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC 
TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG 
AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT 
AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG 
TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA 
ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG 
AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326697.1
CDS	161..7312
Translation	MSEERGKMSLSEKHSVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471022.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471022.1

ORF Insert Sequence:

ATGTCAGAGG AGCGAGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAG ATCAGGTTCG 
TATGTATCCA GCTCTGTGTC TGTGAAGAGT GATGATTCAA AAAAAGGAGG TCAGCCAAAC 
TTCAGTAGAG AAACACTGTC ACCTACCACA AGTGTTCAAA ATGAGTCTTC AGACACAGAC 
ATCCAGACTC AGAAAAAACA CATGAGTTTT AAAGACAATC TTCAGGGGAT CTTCCAGGAT 
CTTGAGAACC AAATATTAAA ATTTCTGAAG AATGAGCTAG AAAAGTTTAA GAAAATATTA 
CATGAAGAAA ATACAGAATA CTTTGTAAAG GACTTTGGTG AGAACAGATG CAGAATAAAA 
GAAGCAGCTC TTGATCTCAC ACTCTACTTC CTGAAAGAAA TGAAGCAAGA TGAAGCAGCA 
GATACTCTAG AAGATGAGCT GTTCTTCATT CATCAGCTGA AATGTGGCCT ACAGAAGAAG 
TATCAGTGTG TGTTTGAAGG AATTGCACAG CAAGGAGACT CCACACTCCT GAAGAACATC 
TACACAGATC TCTATATCAC TCAGGGTGGC AGTGAACAGG TCAATACTGA ACATGAGGTC 
AGGCAGATTG AAGTTACTTC CAGACGTCAT AAAGCTCAAG AGATACAGGT TGAATGCAGA 
CATTTGTTTG AAGCGTCTGA ACAAGACAAG CAGATCAGAA CTGTACTGAC AAAAGGAGTC 
GCTGGCATCG GGAAATCAGT CTCTGTGCAA AAGTTTGTTC TGGATTGGGC TGAAGGAAAA 
GAAAATCAAG AGATCAGCTT CATATTTCCT CTTCCATTCA GAGAGATGAA CTTAAAGGAG 
AAAGAAACAC CCAGTTTGAT GGAGCTTATA ACTCAGTTTT ACCCAGAGAC AAAAGGACTG 
AACCTTACAA GAAGGAATCG ATTCAAAGTC CTGTTCATCC TTGATGGATT GGATGAATGT 
CGTCTTCCTC TGAACTTTGC TGGTAATGAG ACGTGTCGTG ATGTTTCGTC ACCAGCCTCT 
CTGGATGTTC TCCTGACGAA CCTCATCAAG GGAAATCTGC TTCCTTCTGC TCTCATCTGG 
ATCACCAGCA GACCAGCAGC TGCCAGTAAG ATTCCTCCTG ACTGTATCGA CAGAGTGACA 
GAGATACGAG GATTCAATGA TGCACAAAAG GAGGAGTACT TCAGAAAAAG ACTCACAGAT 
GAGAGTCTGG CCAAAGAAAT CATTGATCAT GTTAAAAAAT CAAAGAGTCT TTTCATCATG 
TGCCACATCC CAGTCTTCTG CTGGATTTCA GCCACTGTTC TCCAGAACAT TTTACAGAAG 
AAGAGAAAAA ATGTGAAAAA CAATCAGGCT GATGACGCCT CCAAAACACT GCAGGAATCA 
AACACTGAAG ACACTCCCAA GACTCTGACA CAAATGTACA CACACTTCTT GCACGTTCAG 
ATCAAGCAGA GCAGACAAAA GTATGATGGA GAAAACAAAC CAGATGTGTC CTGGGATAAA 
GACGCCATCC TTTCACTGGG GAAACTGGCA TTTCAGCAGC TGCTAAACAA CAACCTGATC 
TTCTATGAAA CAGACCTGGA AGCCTGTGGT ATTGACGTCT ATAAAGCTGA AAAGTTCTCA 
GGCATGTGTA CTGAGATCTT TAAGGAGGAA ACAGGGATTG TTCTTGGTAC CATGTACTGC 
TTTGTTCACT TGAGTGTTCA AGAGTTTATT GCAGCCCTTT ATGCACATCT GATTCTGAAC 
ATCAACAATA GAAGTGTGTT TGATCAAAAC ACAAGAAATA GAAATGTGTC CATGATTGAT 
TTGCTGAAGA CTGCAGTGGA CAAGGCACTT GAGAGTGACA ATGGACACCT GGACCTTTTC 
CTTCGCTTCC TCCTTGGTCT GTCACTCCAG TCCAACAGTG GAATCTTAAA AGGCCTGTTG 
ACACAGCAAG ACCACAATAA CTGGAGCAAA TGGAAAATAC TTCAGTACTT CTTTAGAACA 
GTAGATCAGC AGCAAGACCA CAATGACCAG ATCAAAGAGG AAATAGTTCA TTACATCAAG 
CAGAAGTTTG AAGCAAATCT TTCTCTTGAG AGATCCATCA ACCTGTTCCA CTGTCTGAAT 
GAACTAAACG ATCAAACTCT GGTGAAAGAG ATTCAGTCTC ACCTTAGCAA AGGAAGGTAC 
TCATCTTCTG ACCTTTCACC TGCCCAGTGG TCTGCTCTGG TCTTTGTGTT GTTGACATCA 
GATGAAGAGA TGGAGGAGTT TGAGCTTCAG AAATTCAAGA AATCAGACGA GTGTCTCATC 
AGGCTATTAG CTGTCATCAA AACCTGCAAA AGAGCACTGC TCAGTAAGTG TAATCTAACA 
GAGGAAAGCT GTTCAGCTCT GGCTTCAGTT CTCAGATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA ACTGTACACT GGAGAAACTC AGACTTTCAG AGTGCGGTAT CAATGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACCATCAGGA GTGAAGGAGC TCACTGATTT ACTGAAACCA 
GACTATACAC TTAAAACACT GAGGTTTTTG AGTCCTGCTG CAGAAGAAGT CTTTCAGTTT 
GTGACTAAAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT 
GAACTAGGAG ATACACGAGT GAATCAACTC TCTGCTCTGC TGCAGGATAA ACACTGTAGA 
CTCAACACAC TCATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTGACT 
TTTGCATTTA ATTTAAATCC TTCACACCTG ATAGAGCTCA ATCTGAGTGG GAATAAACTA 
GGAGACTCAG GAGTAGAGAA GATCTGTCCT CTGCTGGAAA ATACACAATG CCAATTAGAG 
AAACTGAGGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGATTTACAG CAGGATCCAC ACTGTACACT GAAGATACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGCC TGTCAGTTTT TGACTGGAGT TGTGGGTAAA 
AACCTGTTAC TCTTGAAAGA GCTGAATCTG AGTGGACATG AACTAGGACT CTCAGACAGC 
TTTCAAATGC TTGTTCTTTT ACTGCATGAT AAACACTGTA CGCTCAACAC ACTGATTCTT 
TCAGATTGCA GTATCACAGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GACATCAAAC 
CCATCATACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAACTCACTG ATTTACTACT GGATGAACGC TGTAGCTTAA AAATCATCAG GTTTCTGAAA 
AGTCCTGCTG CACAAGAGTC ATGTGATTTT CTCACTGAAG TTCTGGATGT AAATCCATTA 
TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAAATC 
TCTGCTTTTT TGATGGACCC TCATAGCAAA GTGGAGAAAA TCAAGCTGAA TAATTGTAAG 
CTGCTTGAGA AAAGCTCATC AGTTTTAGCA ACAGTTCTGT CTTCCAAAAC CATCCTGAAA 
GAGCTGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAGAT CTGTGAGGGA 
CTGAAGAATC CTGTGTGTGA GCTGAAGATA CTCAAGCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA GTTACTGCAG 
GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCATTACTTC TGAAAGAGCT AAATCTGAGT 
GATCGTGAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGTAAACTCA ACACACTCAT TCTTAGTAAA TGTGGTGTAA CAGTGGAAAG CTGTTCAGCT 
CTGGCTACAG TCCTGAGATC AGAGACCAGT CTAAAAGAGC TTGACATGAG CAACAATAAT 
CTGCAGGAAT CAGGAGTGAA GAAACTCCAG AGCGGATTAA AAAACACAAA CTGCACACTG 
GAAAAACTCA GTCTTTTAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGAAGCT CACTGAGTTA CTGAAACCAG ACTATACACT GAAGACACTG 
AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA 
AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG 
GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT 
AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA 
TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC 
TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG 
TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC 
AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC 
GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT 
CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT 
GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA 
TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA 
TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG 
GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG 
CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC 
AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA 
GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC 
TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT 
GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA 
GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG 
CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG 
GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG 
AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG 
GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC 
TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG 
AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT 
AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG 
TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA 
ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG 
AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471023.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326698.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X5, mRNA.

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CloneID	ODa146927
Clone ID Related Accession (Same CDS sequence)	XM_021471022.1 , XM_021471023.1
Accession Version	XM_021471023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGG AGCGAGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAG ATCAGGTTCG 
TATGTATCCA GCTCTGTGTC TGTGAAGAGT GATGATTCAA AAAAAGGAGG TCAGCCAAAC 
TTCAGTAGAG AAACACTGTC ACCTACCACA AGTGTTCAAA ATGAGTCTTC AGACACAGAC 
ATCCAGACTC AGAAAAAACA CATGAGTTTT AAAGACAATC TTCAGGGGAT CTTCCAGGAT 
CTTGAGAACC AAATATTAAA ATTTCTGAAG AATGAGCTAG AAAAGTTTAA GAAAATATTA 
CATGAAGAAA ATACAGAATA CTTTGTAAAG GACTTTGGTG AGAACAGATG CAGAATAAAA 
GAAGCAGCTC TTGATCTCAC ACTCTACTTC CTGAAAGAAA TGAAGCAAGA TGAAGCAGCA 
GATACTCTAG AAGATGAGCT GTTCTTCATT CATCAGCTGA AATGTGGCCT ACAGAAGAAG 
TATCAGTGTG TGTTTGAAGG AATTGCACAG CAAGGAGACT CCACACTCCT GAAGAACATC 
TACACAGATC TCTATATCAC TCAGGGTGGC AGTGAACAGG TCAATACTGA ACATGAGGTC 
AGGCAGATTG AAGTTACTTC CAGACGTCAT AAAGCTCAAG AGATACAGGT TGAATGCAGA 
CATTTGTTTG AAGCGTCTGA ACAAGACAAG CAGATCAGAA CTGTACTGAC AAAAGGAGTC 
GCTGGCATCG GGAAATCAGT CTCTGTGCAA AAGTTTGTTC TGGATTGGGC TGAAGGAAAA 
GAAAATCAAG AGATCAGCTT CATATTTCCT CTTCCATTCA GAGAGATGAA CTTAAAGGAG 
AAAGAAACAC CCAGTTTGAT GGAGCTTATA ACTCAGTTTT ACCCAGAGAC AAAAGGACTG 
AACCTTACAA GAAGGAATCG ATTCAAAGTC CTGTTCATCC TTGATGGATT GGATGAATGT 
CGTCTTCCTC TGAACTTTGC TGGTAATGAG ACGTGTCGTG ATGTTTCGTC ACCAGCCTCT 
CTGGATGTTC TCCTGACGAA CCTCATCAAG GGAAATCTGC TTCCTTCTGC TCTCATCTGG 
ATCACCAGCA GACCAGCAGC TGCCAGTAAG ATTCCTCCTG ACTGTATCGA CAGAGTGACA 
GAGATACGAG GATTCAATGA TGCACAAAAG GAGGAGTACT TCAGAAAAAG ACTCACAGAT 
GAGAGTCTGG CCAAAGAAAT CATTGATCAT GTTAAAAAAT CAAAGAGTCT TTTCATCATG 
TGCCACATCC CAGTCTTCTG CTGGATTTCA GCCACTGTTC TCCAGAACAT TTTACAGAAG 
AAGAGAAAAA ATGTGAAAAA CAATCAGGCT GATGACGCCT CCAAAACACT GCAGGAATCA 
AACACTGAAG ACACTCCCAA GACTCTGACA CAAATGTACA CACACTTCTT GCACGTTCAG 
ATCAAGCAGA GCAGACAAAA GTATGATGGA GAAAACAAAC CAGATGTGTC CTGGGATAAA 
GACGCCATCC TTTCACTGGG GAAACTGGCA TTTCAGCAGC TGCTAAACAA CAACCTGATC 
TTCTATGAAA CAGACCTGGA AGCCTGTGGT ATTGACGTCT ATAAAGCTGA AAAGTTCTCA 
GGCATGTGTA CTGAGATCTT TAAGGAGGAA ACAGGGATTG TTCTTGGTAC CATGTACTGC 
TTTGTTCACT TGAGTGTTCA AGAGTTTATT GCAGCCCTTT ATGCACATCT GATTCTGAAC 
ATCAACAATA GAAGTGTGTT TGATCAAAAC ACAAGAAATA GAAATGTGTC CATGATTGAT 
TTGCTGAAGA CTGCAGTGGA CAAGGCACTT GAGAGTGACA ATGGACACCT GGACCTTTTC 
CTTCGCTTCC TCCTTGGTCT GTCACTCCAG TCCAACAGTG GAATCTTAAA AGGCCTGTTG 
ACACAGCAAG ACCACAATAA CTGGAGCAAA TGGAAAATAC TTCAGTACTT CTTTAGAACA 
GTAGATCAGC AGCAAGACCA CAATGACCAG ATCAAAGAGG AAATAGTTCA TTACATCAAG 
CAGAAGTTTG AAGCAAATCT TTCTCTTGAG AGATCCATCA ACCTGTTCCA CTGTCTGAAT 
GAACTAAACG ATCAAACTCT GGTGAAAGAG ATTCAGTCTC ACCTTAGCAA AGGAAGGTAC 
TCATCTTCTG ACCTTTCACC TGCCCAGTGG TCTGCTCTGG TCTTTGTGTT GTTGACATCA 
GATGAAGAGA TGGAGGAGTT TGAGCTTCAG AAATTCAAGA AATCAGACGA GTGTCTCATC 
AGGCTATTAG CTGTCATCAA AACCTGCAAA AGAGCACTGC TCAGTAAGTG TAATCTAACA 
GAGGAAAGCT GTTCAGCTCT GGCTTCAGTT CTCAGATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA ACTGTACACT GGAGAAACTC AGACTTTCAG AGTGCGGTAT CAATGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACCATCAGGA GTGAAGGAGC TCACTGATTT ACTGAAACCA 
GACTATACAC TTAAAACACT GAGGTTTTTG AGTCCTGCTG CAGAAGAAGT CTTTCAGTTT 
GTGACTAAAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT 
GAACTAGGAG ATACACGAGT GAATCAACTC TCTGCTCTGC TGCAGGATAA ACACTGTAGA 
CTCAACACAC TCATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTGACT 
TTTGCATTTA ATTTAAATCC TTCACACCTG ATAGAGCTCA ATCTGAGTGG GAATAAACTA 
GGAGACTCAG GAGTAGAGAA GATCTGTCCT CTGCTGGAAA ATACACAATG CCAATTAGAG 
AAACTGAGGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGATTTACAG CAGGATCCAC ACTGTACACT GAAGATACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGCC TGTCAGTTTT TGACTGGAGT TGTGGGTAAA 
AACCTGTTAC TCTTGAAAGA GCTGAATCTG AGTGGACATG AACTAGGACT CTCAGACAGC 
TTTCAAATGC TTGTTCTTTT ACTGCATGAT AAACACTGTA CGCTCAACAC ACTGATTCTT 
TCAGATTGCA GTATCACAGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GACATCAAAC 
CCATCATACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAACTCACTG ATTTACTACT GGATGAACGC TGTAGCTTAA AAATCATCAG GTTTCTGAAA 
AGTCCTGCTG CACAAGAGTC ATGTGATTTT CTCACTGAAG TTCTGGATGT AAATCCATTA 
TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAAATC 
TCTGCTTTTT TGATGGACCC TCATAGCAAA GTGGAGAAAA TCAAGCTGAA TAATTGTAAG 
CTGCTTGAGA AAAGCTCATC AGTTTTAGCA ACAGTTCTGT CTTCCAAAAC CATCCTGAAA 
GAGCTGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAGAT CTGTGAGGGA 
CTGAAGAATC CTGTGTGTGA GCTGAAGATA CTCAAGCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA GTTACTGCAG 
GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCATTACTTC TGAAAGAGCT AAATCTGAGT 
GATCGTGAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGTAAACTCA ACACACTCAT TCTTAGTAAA TGTGGTGTAA CAGTGGAAAG CTGTTCAGCT 
CTGGCTACAG TCCTGAGATC AGAGACCAGT CTAAAAGAGC TTGACATGAG CAACAATAAT 
CTGCAGGAAT CAGGAGTGAA GAAACTCCAG AGCGGATTAA AAAACACAAA CTGCACACTG 
GAAAAACTCA GTCTTTTAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGAAGCT CACTGAGTTA CTGAAACCAG ACTATACACT GAAGACACTG 
AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA 
AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG 
GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT 
AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA 
TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC 
TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG 
TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC 
AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC 
GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT 
CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT 
GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA 
TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA 
TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG 
GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG 
CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC 
AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA 
GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC 
TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT 
GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA 
GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG 
CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG 
GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG 
AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG 
GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC 
TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG 
AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT 
AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG 
TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA 
ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG 
AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326698.1
CDS	178..7329
Translation	MSEERGKMSLSEKHSVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471023.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471023.1

ORF Insert Sequence:

ATGTCAGAGG AGCGAGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAG ATCAGGTTCG 
TATGTATCCA GCTCTGTGTC TGTGAAGAGT GATGATTCAA AAAAAGGAGG TCAGCCAAAC 
TTCAGTAGAG AAACACTGTC ACCTACCACA AGTGTTCAAA ATGAGTCTTC AGACACAGAC 
ATCCAGACTC AGAAAAAACA CATGAGTTTT AAAGACAATC TTCAGGGGAT CTTCCAGGAT 
CTTGAGAACC AAATATTAAA ATTTCTGAAG AATGAGCTAG AAAAGTTTAA GAAAATATTA 
CATGAAGAAA ATACAGAATA CTTTGTAAAG GACTTTGGTG AGAACAGATG CAGAATAAAA 
GAAGCAGCTC TTGATCTCAC ACTCTACTTC CTGAAAGAAA TGAAGCAAGA TGAAGCAGCA 
GATACTCTAG AAGATGAGCT GTTCTTCATT CATCAGCTGA AATGTGGCCT ACAGAAGAAG 
TATCAGTGTG TGTTTGAAGG AATTGCACAG CAAGGAGACT CCACACTCCT GAAGAACATC 
TACACAGATC TCTATATCAC TCAGGGTGGC AGTGAACAGG TCAATACTGA ACATGAGGTC 
AGGCAGATTG AAGTTACTTC CAGACGTCAT AAAGCTCAAG AGATACAGGT TGAATGCAGA 
CATTTGTTTG AAGCGTCTGA ACAAGACAAG CAGATCAGAA CTGTACTGAC AAAAGGAGTC 
GCTGGCATCG GGAAATCAGT CTCTGTGCAA AAGTTTGTTC TGGATTGGGC TGAAGGAAAA 
GAAAATCAAG AGATCAGCTT CATATTTCCT CTTCCATTCA GAGAGATGAA CTTAAAGGAG 
AAAGAAACAC CCAGTTTGAT GGAGCTTATA ACTCAGTTTT ACCCAGAGAC AAAAGGACTG 
AACCTTACAA GAAGGAATCG ATTCAAAGTC CTGTTCATCC TTGATGGATT GGATGAATGT 
CGTCTTCCTC TGAACTTTGC TGGTAATGAG ACGTGTCGTG ATGTTTCGTC ACCAGCCTCT 
CTGGATGTTC TCCTGACGAA CCTCATCAAG GGAAATCTGC TTCCTTCTGC TCTCATCTGG 
ATCACCAGCA GACCAGCAGC TGCCAGTAAG ATTCCTCCTG ACTGTATCGA CAGAGTGACA 
GAGATACGAG GATTCAATGA TGCACAAAAG GAGGAGTACT TCAGAAAAAG ACTCACAGAT 
GAGAGTCTGG CCAAAGAAAT CATTGATCAT GTTAAAAAAT CAAAGAGTCT TTTCATCATG 
TGCCACATCC CAGTCTTCTG CTGGATTTCA GCCACTGTTC TCCAGAACAT TTTACAGAAG 
AAGAGAAAAA ATGTGAAAAA CAATCAGGCT GATGACGCCT CCAAAACACT GCAGGAATCA 
AACACTGAAG ACACTCCCAA GACTCTGACA CAAATGTACA CACACTTCTT GCACGTTCAG 
ATCAAGCAGA GCAGACAAAA GTATGATGGA GAAAACAAAC CAGATGTGTC CTGGGATAAA 
GACGCCATCC TTTCACTGGG GAAACTGGCA TTTCAGCAGC TGCTAAACAA CAACCTGATC 
TTCTATGAAA CAGACCTGGA AGCCTGTGGT ATTGACGTCT ATAAAGCTGA AAAGTTCTCA 
GGCATGTGTA CTGAGATCTT TAAGGAGGAA ACAGGGATTG TTCTTGGTAC CATGTACTGC 
TTTGTTCACT TGAGTGTTCA AGAGTTTATT GCAGCCCTTT ATGCACATCT GATTCTGAAC 
ATCAACAATA GAAGTGTGTT TGATCAAAAC ACAAGAAATA GAAATGTGTC CATGATTGAT 
TTGCTGAAGA CTGCAGTGGA CAAGGCACTT GAGAGTGACA ATGGACACCT GGACCTTTTC 
CTTCGCTTCC TCCTTGGTCT GTCACTCCAG TCCAACAGTG GAATCTTAAA AGGCCTGTTG 
ACACAGCAAG ACCACAATAA CTGGAGCAAA TGGAAAATAC TTCAGTACTT CTTTAGAACA 
GTAGATCAGC AGCAAGACCA CAATGACCAG ATCAAAGAGG AAATAGTTCA TTACATCAAG 
CAGAAGTTTG AAGCAAATCT TTCTCTTGAG AGATCCATCA ACCTGTTCCA CTGTCTGAAT 
GAACTAAACG ATCAAACTCT GGTGAAAGAG ATTCAGTCTC ACCTTAGCAA AGGAAGGTAC 
TCATCTTCTG ACCTTTCACC TGCCCAGTGG TCTGCTCTGG TCTTTGTGTT GTTGACATCA 
GATGAAGAGA TGGAGGAGTT TGAGCTTCAG AAATTCAAGA AATCAGACGA GTGTCTCATC 
AGGCTATTAG CTGTCATCAA AACCTGCAAA AGAGCACTGC TCAGTAAGTG TAATCTAACA 
GAGGAAAGCT GTTCAGCTCT GGCTTCAGTT CTCAGATCAG ACTCCAGCAG TCTGAAGGAT 
CTTGACCTGA GCAATAATAA TCTGCAGGAT TCAGGAGTGA AGCTGCTCTC TGATGGACTG 
AAGGACAGTA ACTGTACACT GGAGAAACTC AGACTTTCAG AGTGCGGTAT CAATGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACCATCAGGA GTGAAGGAGC TCACTGATTT ACTGAAACCA 
GACTATACAC TTAAAACACT GAGGTTTTTG AGTCCTGCTG CAGAAGAAGT CTTTCAGTTT 
GTGACTAAAA TTGTGGGTAA AAACCCGTTA CTCCTGAAAG AACTGAATCT GAGTGATCGT 
GAACTAGGAG ATACACGAGT GAATCAACTC TCTGCTCTGC TGCAGGATAA ACACTGTAGA 
CTCAACACAC TCATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTGACT 
TTTGCATTTA ATTTAAATCC TTCACACCTG ATAGAGCTCA ATCTGAGTGG GAATAAACTA 
GGAGACTCAG GAGTAGAGAA GATCTGTCCT CTGCTGGAAA ATACACAATG CCAATTAGAG 
AAACTGAGGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGATTTACAG CAGGATCCAC ACTGTACACT GAAGATACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGCC TGTCAGTTTT TGACTGGAGT TGTGGGTAAA 
AACCTGTTAC TCTTGAAAGA GCTGAATCTG AGTGGACATG AACTAGGACT CTCAGACAGC 
TTTCAAATGC TTGTTCTTTT ACTGCATGAT AAACACTGTA CGCTCAACAC ACTGATTCTT 
TCAGATTGCA GTATCACAGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GACATCAAAC 
CCATCATACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAACTCACTG ATTTACTACT GGATGAACGC TGTAGCTTAA AAATCATCAG GTTTCTGAAA 
AGTCCTGCTG CACAAGAGTC ATGTGATTTT CTCACTGAAG TTCTGGATGT AAATCCATTA 
TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAAATC 
TCTGCTTTTT TGATGGACCC TCATAGCAAA GTGGAGAAAA TCAAGCTGAA TAATTGTAAG 
CTGCTTGAGA AAAGCTCATC AGTTTTAGCA ACAGTTCTGT CTTCCAAAAC CATCCTGAAA 
GAGCTGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAGAT CTGTGAGGGA 
CTGAAGAATC CTGTGTGTGA GCTGAAGATA CTCAAGCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA GTTACTGCAG 
GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCATTACTTC TGAAAGAGCT AAATCTGAGT 
GATCGTGAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGTAAACTCA ACACACTCAT TCTTAGTAAA TGTGGTGTAA CAGTGGAAAG CTGTTCAGCT 
CTGGCTACAG TCCTGAGATC AGAGACCAGT CTAAAAGAGC TTGACATGAG CAACAATAAT 
CTGCAGGAAT CAGGAGTGAA GAAACTCCAG AGCGGATTAA AAAACACAAA CTGCACACTG 
GAAAAACTCA GTCTTTTAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGAAGCT CACTGAGTTA CTGAAACCAG ACTATACACT GAAGACACTG 
AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA 
AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG 
GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT 
AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA 
TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC 
TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG 
TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC 
AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC 
GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT 
CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT 
GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA 
TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA 
TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG 
GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG 
CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC 
AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA 
GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC 
TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT 
GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA 
GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG 
CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG 
GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG 
AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG 
GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC 
TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG 
AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT 
AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG 
TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA 
ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG 
AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471024.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326699.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X6, mRNA.

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CloneID	ODa146928
Clone ID Related Accession (Same CDS sequence)	XM_021471024.1
Accession Version	XM_021471024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCT CAAAAAAACA CAGTGTAAGA TCAGGCTCAC ATGTGTCCAG CTCTGTGTCT 
GTGAAGAGTG ACCAGTCAAA AGGTCACATG CCAAACTTCA GTGAGACAAA AACAAAATCA 
AATAAAACGT TTCAATATAA GACATTAGAA TCTGACTTCC ATCCTCAAAG GAGCTGCAAT 
AATTTCAAAG ACCTTCAGTG CATCTTTAAG GGGCTTGAGA GCAAAATGTT CAACTTCCTG 
AAGAAAGAGT TGGAAAATTT TAAGGAAATC TTACGAAAAG AAAACACACA ATACCTTGTA 
AAGGATTTTA ATAAGAACAG ATGCAGCATC AGAGAAACAG CTCTTGATCT TACACTTTAC 
TTCCTGAGAG AAATGAAGCA AGATGAAGCT GCAGATACTC TAGAAGATGA GCTGTTCTTC 
ATTCATCAGC TGAAATGTGG CCTACAGAAG AAGTATCAGT GTGTGTTTGA AGGAATTGCA 
CAGCAAGGAG ACTCCACACT CCTGAAGAAC ATCTACACAG ATCTCTATAT CACTCAGGGT 
GGCAGTGAAC AGGTCAATAC TGAACATGAG GTCAGGCAGA TTGAAGTTAC TTCCAGACGT 
CATAAAGCTC AAGAGATACA GGTTGAATGC AGACATTTGT TTGAAGCGTC TGAACAAGAC 
AAGCAGATCA GAACTGTACT GACAAAAGGA GTCGCTGGCA TCGGGAAATC AGTCTCTGTG 
CAAAAGTTTG TTCTGGATTG GGCTGAAGGA AAAGAAAATC AAGAGATCAG CTTCATATTT 
CCTCTTCCAT TCAGAGAGAT GAACTTAAAG GAGAAAGAAA CACCCAGTTT GATGGAGCTT 
ATAACTCAGT TTTACCCAGA GACAAAAGGA CTGAACCTTA CAAGAAGGAA TCGATTCAAA 
GTCCTGTTCA TCCTTGATGG ATTGGATGAA TGTCGTCTTC CTCTGAACTT TGCTGGTAAT 
GAGACGTGTC GTGATGTTTC GTCACCAGCC TCTCTGGATG TTCTCCTGAC GAACCTCATC 
AAGGGAAATC TGCTTCCTTC TGCTCTCATC TGGATCACCA GCAGACCAGC AGCTGCCAGT 
AAGATTCCTC CTGACTGTAT CGACAGAGTG ACAGAGATAC GAGGATTCAA TGATGCACAA 
AAGGAGGAGT ACTTCAGAAA AAGACTCACA GATGAGAGTC TGGCCAAAGA AATCATTGAT 
CATGTTAAAA AATCAAAGAG TCTTTTCATC ATGTGCCACA TCCCAGTCTT CTGCTGGATT 
TCAGCCACTG TTCTCCAGAA CATTTTACAG AAGAAGAGAA AAAATGTGAA AAACAATCAG 
GCTGATGACG CCTCCAAAAC ACTGCAGGAA TCAAACACTG AAGACACTCC CAAGACTCTG 
ACACAAATGT ACACACACTT CTTGCACGTT CAGATCAAGC AGAGCAGACA AAAGTATGAT 
GGAGAAAACA AACCAGATGT GTCCTGGGAT AAAGACGCCA TCCTTTCACT GGGGAAACTG 
GCATTTCAGC AGCTGCTAAA CAACAACCTG ATCTTCTATG AAACAGACCT GGAAGCCTGT 
GGTATTGACG TCTATAAAGC TGAAAAGTTC TCAGGCATGT GTACTGAGAT CTTTAAGGAG 
GAAACAGGGA TTGTTCTTGG TACCATGTAC TGCTTTGTTC ACTTGAGTGT TCAAGAGTTT 
ATTGCAGCCC TTTATGCACA TCTGATTCTG AACATCAACA ATAGAAGTGT GTTTGATCAA 
AACACAAGAA ATAGAAATGT GTCCATGATT GATTTGCTGA AGACTGCAGT GGACAAGGCA 
CTTGAGAGTG ACAATGGACA CCTGGACCTT TTCCTTCGCT TCCTCCTTGG TCTGTCACTC 
CAGTCCAACA GTGGAATCTT AAAAGGCCTG TTGACACAGC AAGACCACAA TAACTGGAGC 
AAATGGAAAA TACTTCAGTA CTTCTTTAGA ACAGTAGATC AGCAGCAAGA CCACAATGAC 
CAGATCAAAG AGGAAATAGT TCATTACATC AAGCAGAAGT TTGAAGCAAA TCTTTCTCTT 
GAGAGATCCA TCAACCTGTT CCACTGTCTG AATGAACTAA ACGATCAAAC TCTGGTGAAA 
GAGATTCAGT CTCACCTTAG CAAAGGAAGG TACTCATCTT CTGACCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGTCTTTGT GTTGTTGACA TCAGATGAAG AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGGCTAT TAGCTGTCAT CAAAACCTGC 
AAAAGAGCAC TGCTCAGTAA GTGTAATCTA ACAGAGGAAA GCTGTTCAGC TCTGGCTTCA 
GTTCTCAGAT CAGACTCCAG CAGTCTGAAG GATCTTGACC TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTCTGATGGA CTGAAGGACA GTAACTGTAC ACTGGAGAAA 
CTCAGACTTT CAGAGTGCGG TATCAATGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACCATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGAAA CCAGACTATA CACTTAAAAC ACTGAGGTTT 
TTGAGTCCTG CTGCAGAAGA AGTCTTTCAG TTTGTGACTA AAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTGAACTAG GAGATACACG AGTGAATCAA 
CTCTCTGCTC TGCTGCAGGA TAAACACTGT AGACTCAACA CACTCATGCT GAATAATAAC 
AGGATTACAG CAGAAGGTTG TGCTGCTTTG ACTTTTGCAT TTAATTTAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGGGAATAAA CTAGGAGACT CAGGAGTAGA GAAGATCTGT 
CCTCTGCTGG AAAATACACA ATGCCAATTA GAGAAACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG 
TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA 
GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT 
CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT 
CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG 
TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT 
TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT 
AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG 
CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GACGCCTGTC GGTTTGTGAC TAAAATTGTG 
GGTAAAAACC CATTACTCCT GACAGAACTG AATCTGAGTG ATCGTAAACT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GTAGAGTCAA CACAATAATG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTATTTTA 
AACCCATCAC ACCTGATAGA GCTCAATCTG AGTGGGAATA AACTAGGAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GGAAAATACA CAATGCCGAT TAGAGAAACT GAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGAAG 
CTCACTGATT TACTGCACAG TAGAAGCTGT AAATTCAGAT TTCTGAAGAG TTCTGCTGCA 
CAGGAAGCAT GTGACTATCT TACTAAAGTT CTGGGTATAA GTCCATTATT ACTGACAGAA 
CTGGATCTGA GTGAAGATAA ATTAGGAGAT CTGGATGGAG AGAAACTCTC TGCTCTTCTG 
ATGGACTCTC ATAGCAAAGT GAAGAAAATC AAGCTGAATA ATTGTGAGCT GACAGAGAAA 
AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC TCCAAAACCA TCCTGAAAGA GATGAACCTG 
AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAGAAATCT GTGAGGGACT GAAGAATCCT 
GTGTGTGAGC TGAAAATACT CAGTCTTTCA GACTGCCGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGACT TACTGGAGGA TCCACACTGT 
ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AAGGCTGTCA CTTTGTGACT 
GAAATGGTGG GTAAAAACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGTACTA 
GGAGACACAC GAGTGAATCA ACTCTCTGCT CTGCTGCAGG ATAAACACTG TCAGATCCAC 
ACACTCATTC TGTGTTATTG CAGTATTACA GAGGAACAGT GTGTGATCCT GACTTCAGCT 
CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGAAACAA TCTAGGAGAC 
TCTGCAGTGA AAAAACTCAG TGATTTACTG ATGAACCAAC AATTCAAGCT GGAGAAACTA 
CGTCTGAGTG ATTGCAGTAT TACAGAGAAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAATGGGA ATAGACTAGG AGACTCTGGA 
GCTTATGGAC TTGGCCATCT GCTGAGCAGT GTGGATTGCA AGCTGAATAA ACTACATCTG 
TGTGATTGCA GTATTACAGG GAAACAGTTG CACTTCCTGA TTGCAGTTAT GTGTATGGAC 
CCATCACACC TGAGGGTACT GGACCTCAGT AAGAATCAAA TAAAAAGCAG AGGAATGAAC 
ATGCTGTGTG ATGTGCTGAA TAAACCACGC TGTAAACTCA AGAGTTTAAG ACTAAATGAC 
TGTGGCATTA CAGATGTTAC ACACTTAACT CAGTCTTTGG CTGAGAACAA AGCACTGGAG 
TTCTTAAAAG AGCTTGAACT GAGTAAAAAT CAAATGGGAG ACTCCGAAAA GCAAAAACTC 
AGTGACCTGC TGCGAGACTC AAACTGTAAA CTGAGGTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326699.1
CDS	281..7405
Translation	MSSSKKHSVRSGSHVSSSVSVKSDQSKGHMPNFSETKTKSNKTFQYKTLESDFHPQRSCNNFKDLQCIFKGLESKMFNFLKKELENFKEILRKENTQYLVKDFNKNRCSIRETALDLTLYFLREMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471024.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471024.1

ORF Insert Sequence:

ATGAGTTCCT CAAAAAAACA CAGTGTAAGA TCAGGCTCAC ATGTGTCCAG CTCTGTGTCT 
GTGAAGAGTG ACCAGTCAAA AGGTCACATG CCAAACTTCA GTGAGACAAA AACAAAATCA 
AATAAAACGT TTCAATATAA GACATTAGAA TCTGACTTCC ATCCTCAAAG GAGCTGCAAT 
AATTTCAAAG ACCTTCAGTG CATCTTTAAG GGGCTTGAGA GCAAAATGTT CAACTTCCTG 
AAGAAAGAGT TGGAAAATTT TAAGGAAATC TTACGAAAAG AAAACACACA ATACCTTGTA 
AAGGATTTTA ATAAGAACAG ATGCAGCATC AGAGAAACAG CTCTTGATCT TACACTTTAC 
TTCCTGAGAG AAATGAAGCA AGATGAAGCT GCAGATACTC TAGAAGATGA GCTGTTCTTC 
ATTCATCAGC TGAAATGTGG CCTACAGAAG AAGTATCAGT GTGTGTTTGA AGGAATTGCA 
CAGCAAGGAG ACTCCACACT CCTGAAGAAC ATCTACACAG ATCTCTATAT CACTCAGGGT 
GGCAGTGAAC AGGTCAATAC TGAACATGAG GTCAGGCAGA TTGAAGTTAC TTCCAGACGT 
CATAAAGCTC AAGAGATACA GGTTGAATGC AGACATTTGT TTGAAGCGTC TGAACAAGAC 
AAGCAGATCA GAACTGTACT GACAAAAGGA GTCGCTGGCA TCGGGAAATC AGTCTCTGTG 
CAAAAGTTTG TTCTGGATTG GGCTGAAGGA AAAGAAAATC AAGAGATCAG CTTCATATTT 
CCTCTTCCAT TCAGAGAGAT GAACTTAAAG GAGAAAGAAA CACCCAGTTT GATGGAGCTT 
ATAACTCAGT TTTACCCAGA GACAAAAGGA CTGAACCTTA CAAGAAGGAA TCGATTCAAA 
GTCCTGTTCA TCCTTGATGG ATTGGATGAA TGTCGTCTTC CTCTGAACTT TGCTGGTAAT 
GAGACGTGTC GTGATGTTTC GTCACCAGCC TCTCTGGATG TTCTCCTGAC GAACCTCATC 
AAGGGAAATC TGCTTCCTTC TGCTCTCATC TGGATCACCA GCAGACCAGC AGCTGCCAGT 
AAGATTCCTC CTGACTGTAT CGACAGAGTG ACAGAGATAC GAGGATTCAA TGATGCACAA 
AAGGAGGAGT ACTTCAGAAA AAGACTCACA GATGAGAGTC TGGCCAAAGA AATCATTGAT 
CATGTTAAAA AATCAAAGAG TCTTTTCATC ATGTGCCACA TCCCAGTCTT CTGCTGGATT 
TCAGCCACTG TTCTCCAGAA CATTTTACAG AAGAAGAGAA AAAATGTGAA AAACAATCAG 
GCTGATGACG CCTCCAAAAC ACTGCAGGAA TCAAACACTG AAGACACTCC CAAGACTCTG 
ACACAAATGT ACACACACTT CTTGCACGTT CAGATCAAGC AGAGCAGACA AAAGTATGAT 
GGAGAAAACA AACCAGATGT GTCCTGGGAT AAAGACGCCA TCCTTTCACT GGGGAAACTG 
GCATTTCAGC AGCTGCTAAA CAACAACCTG ATCTTCTATG AAACAGACCT GGAAGCCTGT 
GGTATTGACG TCTATAAAGC TGAAAAGTTC TCAGGCATGT GTACTGAGAT CTTTAAGGAG 
GAAACAGGGA TTGTTCTTGG TACCATGTAC TGCTTTGTTC ACTTGAGTGT TCAAGAGTTT 
ATTGCAGCCC TTTATGCACA TCTGATTCTG AACATCAACA ATAGAAGTGT GTTTGATCAA 
AACACAAGAA ATAGAAATGT GTCCATGATT GATTTGCTGA AGACTGCAGT GGACAAGGCA 
CTTGAGAGTG ACAATGGACA CCTGGACCTT TTCCTTCGCT TCCTCCTTGG TCTGTCACTC 
CAGTCCAACA GTGGAATCTT AAAAGGCCTG TTGACACAGC AAGACCACAA TAACTGGAGC 
AAATGGAAAA TACTTCAGTA CTTCTTTAGA ACAGTAGATC AGCAGCAAGA CCACAATGAC 
CAGATCAAAG AGGAAATAGT TCATTACATC AAGCAGAAGT TTGAAGCAAA TCTTTCTCTT 
GAGAGATCCA TCAACCTGTT CCACTGTCTG AATGAACTAA ACGATCAAAC TCTGGTGAAA 
GAGATTCAGT CTCACCTTAG CAAAGGAAGG TACTCATCTT CTGACCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGTCTTTGT GTTGTTGACA TCAGATGAAG AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGGCTAT TAGCTGTCAT CAAAACCTGC 
AAAAGAGCAC TGCTCAGTAA GTGTAATCTA ACAGAGGAAA GCTGTTCAGC TCTGGCTTCA 
GTTCTCAGAT CAGACTCCAG CAGTCTGAAG GATCTTGACC TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTCTGATGGA CTGAAGGACA GTAACTGTAC ACTGGAGAAA 
CTCAGACTTT CAGAGTGCGG TATCAATGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACCATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGAAA CCAGACTATA CACTTAAAAC ACTGAGGTTT 
TTGAGTCCTG CTGCAGAAGA AGTCTTTCAG TTTGTGACTA AAATTGTGGG TAAAAACCCG 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTGAACTAG GAGATACACG AGTGAATCAA 
CTCTCTGCTC TGCTGCAGGA TAAACACTGT AGACTCAACA CACTCATGCT GAATAATAAC 
AGGATTACAG CAGAAGGTTG TGCTGCTTTG ACTTTTGCAT TTAATTTAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGGGAATAAA CTAGGAGACT CAGGAGTAGA GAAGATCTGT 
CCTCTGCTGG AAAATACACA ATGCCAATTA GAGAAACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG 
TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA 
GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT 
CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT 
CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG 
TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT 
TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT 
AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG 
CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GACGCCTGTC GGTTTGTGAC TAAAATTGTG 
GGTAAAAACC CATTACTCCT GACAGAACTG AATCTGAGTG ATCGTAAACT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GTAGAGTCAA CACAATAATG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTATTTTA 
AACCCATCAC ACCTGATAGA GCTCAATCTG AGTGGGAATA AACTAGGAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GGAAAATACA CAATGCCGAT TAGAGAAACT GAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGAAG 
CTCACTGATT TACTGCACAG TAGAAGCTGT AAATTCAGAT TTCTGAAGAG TTCTGCTGCA 
CAGGAAGCAT GTGACTATCT TACTAAAGTT CTGGGTATAA GTCCATTATT ACTGACAGAA 
CTGGATCTGA GTGAAGATAA ATTAGGAGAT CTGGATGGAG AGAAACTCTC TGCTCTTCTG 
ATGGACTCTC ATAGCAAAGT GAAGAAAATC AAGCTGAATA ATTGTGAGCT GACAGAGAAA 
AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC TCCAAAACCA TCCTGAAAGA GATGAACCTG 
AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAGAAATCT GTGAGGGACT GAAGAATCCT 
GTGTGTGAGC TGAAAATACT CAGTCTTTCA GACTGCCGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGACT TACTGGAGGA TCCACACTGT 
ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AAGGCTGTCA CTTTGTGACT 
GAAATGGTGG GTAAAAACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGTACTA 
GGAGACACAC GAGTGAATCA ACTCTCTGCT CTGCTGCAGG ATAAACACTG TCAGATCCAC 
ACACTCATTC TGTGTTATTG CAGTATTACA GAGGAACAGT GTGTGATCCT GACTTCAGCT 
CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGAAACAA TCTAGGAGAC 
TCTGCAGTGA AAAAACTCAG TGATTTACTG ATGAACCAAC AATTCAAGCT GGAGAAACTA 
CGTCTGAGTG ATTGCAGTAT TACAGAGAAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAATGGGA ATAGACTAGG AGACTCTGGA 
GCTTATGGAC TTGGCCATCT GCTGAGCAGT GTGGATTGCA AGCTGAATAA ACTACATCTG 
TGTGATTGCA GTATTACAGG GAAACAGTTG CACTTCCTGA TTGCAGTTAT GTGTATGGAC 
CCATCACACC TGAGGGTACT GGACCTCAGT AAGAATCAAA TAAAAAGCAG AGGAATGAAC 
ATGCTGTGTG ATGTGCTGAA TAAACCACGC TGTAAACTCA AGAGTTTAAG ACTAAATGAC 
TGTGGCATTA CAGATGTTAC ACACTTAACT CAGTCTTTGG CTGAGAACAA AGCACTGGAG 
TTCTTAAAAG AGCTTGAACT GAGTAAAAAT CAAATGGGAG ACTCCGAAAA GCAAAAACTC 
AGTGACCTGC TGCGAGACTC AAACTGTAAA CTGAGGTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471025.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326700.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X7, mRNA.

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CloneID	ODa146929
Clone ID Related Accession (Same CDS sequence)	XM_021471025.1
Accession Version	XM_021471025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG 
ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA 
CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT 
GAAGCCTGTA CACATCTGAA TAAAGTTCTG GGTGGAAACC TGTTACTCTT GAGAGAGCTG 
AATCTGAGTA AACTTGATCT TGGACCATCA GGATTGGAGA AACTTGCTGC TGTACTGAAA 
GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCAGTATGTC AGAAGAAGAT 
TGTCGTGTTT TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGACAGA GCTGAATTTG 
AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CCACTCTGTT TCAAAACCAA 
CAGTGTAGAC TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT 
GCTTTGGCTT CAGCATTTAA CTCAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGAG 
AATAAACTAG GAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCGCAGTGC 
ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGGATCCACA CTGTACACTG 
AAGACACTGA GTCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGAAGCT CACTGATTTA CTGCACAGTA GAAGCTGTAA ATTCAGATTT 
CTGAAGAGTT CTGCTGCACA GGAAGCATGT GACTATCTTA CTAAAGTTCT GGGTATAAGT 
CCATTATTAC TGACAGAACT GGATCTGAGT GAAGATAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAT GGACTCTCAT AGCAAAGTGA AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCACTT CTAGCTGCAG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAAATCTGT 
GAGGGACTGA AGAATCCTGT GTGTGAGCTG AAAATACTCA GTCTTTCAGA CTGCCGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGACTTA 
CTGGAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GGCTGTCACT TTGTGACTGA AATGGTGGGT AAAAACCCAT TACTCCTGAA AGAACTGAAT 
CTGAGTGATC GTGTACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT GCTGCAGGAT 
AAACACTGTC AGATCCACAC ACTCATTCTG TGTTATTGCA GTATTACAGA GGAACAGTGT 
GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAGT 
GGAAACAATC TAGGAGACTC TGCAGTGAAA AAACTCAGTG ATTTACTGAT GAACCAACAA 
TTCAAGCTGG AGAAACTACG TCTGAGTGAT TGCAGTATTA CAGAGAAACA GTGTGTGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAATGGGAAT 
AGACTAGGAG ACTCTGGAGC TTATGGACTT GGCCATCTGC TGAGCAGTGT GGATTGCAAG 
CTGAATAAAC TACATCTGTG TGATTGCAGT ATTACAGGGA AACAGTTGCA CTTCCTGATT 
GCAGTTATGT GTATGGACCC ATCACACCTG AGGGTACTGG ACCTCAGTAA GAATCAAATA 
AAAAGCAGAG GAATGAACAT GCTGTGTGAT GTGCTGAATA AACCACGCTG TAAACTCAAG 
AGTTTAAGAC TAAATGACTG TGGCATTACA GATGTTACAC ACTTAACTCA GTCTTTGGCT 
GAGAACAAAG CACTGGAGTT CTTAAAAGAG CTTGAACTGA GTAAAAATCA AATGGGAGAC 
TCCGAAAAGC AAAAACTCAG TGACCTGCTG CGAGACTCAA ACTGTAAACT GAGGTTAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326700.1
CDS	182..7144
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471025.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471025.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG 
ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA 
CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT 
GAAGCCTGTA CACATCTGAA TAAAGTTCTG GGTGGAAACC TGTTACTCTT GAGAGAGCTG 
AATCTGAGTA AACTTGATCT TGGACCATCA GGATTGGAGA AACTTGCTGC TGTACTGAAA 
GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCAGTATGTC AGAAGAAGAT 
TGTCGTGTTT TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGACAGA GCTGAATTTG 
AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CCACTCTGTT TCAAAACCAA 
CAGTGTAGAC TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT 
GCTTTGGCTT CAGCATTTAA CTCAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGAG 
AATAAACTAG GAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCGCAGTGC 
ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGGATCCACA CTGTACACTG 
AAGACACTGA GTCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGAAGCT CACTGATTTA CTGCACAGTA GAAGCTGTAA ATTCAGATTT 
CTGAAGAGTT CTGCTGCACA GGAAGCATGT GACTATCTTA CTAAAGTTCT GGGTATAAGT 
CCATTATTAC TGACAGAACT GGATCTGAGT GAAGATAAAT TAGGAGATCT GGATGGAGAG 
AAACTCTCTG CTCTTCTGAT GGACTCTCAT AGCAAAGTGA AGAAAATCAA GCTGAATAAT 
TGTGAGCTGA CAGAGAAAAG CTGTTCACTT CTAGCTGCAG TTCTCTCCTC CAAAACCATC 
CTGAAAGAGA TGAACCTGAA CAACAGTCGT CTGCTGGACT CAGGAGTCAG AGAAATCTGT 
GAGGGACTGA AGAATCCTGT GTGTGAGCTG AAAATACTCA GTCTTTCAGA CTGCCGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGACTTA 
CTGGAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GGCTGTCACT TTGTGACTGA AATGGTGGGT AAAAACCCAT TACTCCTGAA AGAACTGAAT 
CTGAGTGATC GTGTACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT GCTGCAGGAT 
AAACACTGTC AGATCCACAC ACTCATTCTG TGTTATTGCA GTATTACAGA GGAACAGTGT 
GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAGT 
GGAAACAATC TAGGAGACTC TGCAGTGAAA AAACTCAGTG ATTTACTGAT GAACCAACAA 
TTCAAGCTGG AGAAACTACG TCTGAGTGAT TGCAGTATTA CAGAGAAACA GTGTGTGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAATGGGAAT 
AGACTAGGAG ACTCTGGAGC TTATGGACTT GGCCATCTGC TGAGCAGTGT GGATTGCAAG 
CTGAATAAAC TACATCTGTG TGATTGCAGT ATTACAGGGA AACAGTTGCA CTTCCTGATT 
GCAGTTATGT GTATGGACCC ATCACACCTG AGGGTACTGG ACCTCAGTAA GAATCAAATA 
AAAAGCAGAG GAATGAACAT GCTGTGTGAT GTGCTGAATA AACCACGCTG TAAACTCAAG 
AGTTTAAGAC TAAATGACTG TGGCATTACA GATGTTACAC ACTTAACTCA GTCTTTGGCT 
GAGAACAAAG CACTGGAGTT CTTAAAAGAG CTTGAACTGA GTAAAAATCA AATGGGAGAC 
TCCGAAAAGC AAAAACTCAG TGACCTGCTG CGAGACTCAA ACTGTAAACT GAGGTTAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471026.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326701.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X8, mRNA.

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CloneID	ODa146930
Clone ID Related Accession (Same CDS sequence)	XM_021471026.1
Accession Version	XM_021471026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTGATGA TGCAGATGAA GCCTGTACAC ATCTGAATAA AGTTCTGGGT 
GGAAACCTGT TACTCTTGAG AGAGCTGAAT CTGAGTAAAC TTGATCTTGG ACCATCAGGA 
TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT AAACACTGTA AACTCAACAC ACTGATTCTG 
AATAACAGCA GTATGTCAGA AGAAGATTGT CGTGTTTTGG CTGAAGCTTT TAATTCAAAC 
CCATCAAATC TGACAGAGCT GAATTTGAGT GGGACTAAAC TCACAAACTC AGGAATGAAG 
AGCTTCACCA CTCTGTTTCA AAACCAACAG TGTAGACTGG AAAAGCTGAA GTTCAGCAGC 
ATGTGTATTA CAGCAGAAGG TTGTGCTGCT TTGGCTTCAG CATTTAACTC AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGAGAAT AAACTAGGAG ACTCAGGAGT AACAGAAATC 
TCCAGTCTAC TGGCAAACTC GCAGTGCATG CTGAAGATAC TCGGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GACGCCTGTC GGTTTGTGAC TAAAATTGTG GGTAAAAACC CATTACTCCT GACAGAACTG 
AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAGAGTCAA CACAATAATG CTGAATAATA ACAGGATTAC AGCAGAAGGT 
TGTGCTGCTT TAACTTCAGC ATTTATTTTA AACCCATCAC ACCTGATAGA GCTCAATCTG 
AGTGGGAATA AACTAGGAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATACA 
CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGAAG CTCACTGATT TACTGCACAG TAGAAGCTGT 
AAATTCAGAT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GTGACTATCT TACTAAAGTT 
CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT 
CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GAAGAAAATC 
AAGCTGAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC 
TCCAAAACCA TCCTGAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
AGAGAAATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAAATACT CAGTCTTTCA 
GACTGCCGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGACT TACTGGAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG AAGGCTGTCA CTTTGTGACT GAAATGGTGG GTAAAAACCC ATTACTCCTG 
AAAGAACTGA ATCTGAGTGA TCGTGTACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT 
CTGCTGCAGG ATAAACACTG TCAGATCCAC ACACTCATTC TGTGTTATTG CAGTATTACA 
GAGGAACAGT GTGTGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA 
CTGAACCTCA GTGGAAACAA TCTAGGAGAC TCTGCAGTGA AAAAACTCAG TGATTTACTG 
ATGAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTG ATTGCAGTAT TACAGAGAAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTTATGGAC TTGGCCATCT GCTGAGCAGT 
GTGGATTGCA AGCTGAATAA ACTACATCTG TGTGATTGCA GTATTACAGG GAAACAGTTG 
CACTTCCTGA TTGCAGTTAT GTGTATGGAC CCATCACACC TGAGGGTACT GGACCTCAGT 
AAGAATCAAA TAAAAAGCAG AGGAATGAAC ATGCTGTGTG ATGTGCTGAA TAAACCACGC 
TGTAAACTCA AGAGTTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACACTTAACT 
CAGTCTTTGG CTGAGAACAA AGCACTGGAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT 
CAAATGGGAG ACTCCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA 
CTGAGGTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326701.1
CDS	182..6976
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471026.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471026.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTGATGA TGCAGATGAA GCCTGTACAC ATCTGAATAA AGTTCTGGGT 
GGAAACCTGT TACTCTTGAG AGAGCTGAAT CTGAGTAAAC TTGATCTTGG ACCATCAGGA 
TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT AAACACTGTA AACTCAACAC ACTGATTCTG 
AATAACAGCA GTATGTCAGA AGAAGATTGT CGTGTTTTGG CTGAAGCTTT TAATTCAAAC 
CCATCAAATC TGACAGAGCT GAATTTGAGT GGGACTAAAC TCACAAACTC AGGAATGAAG 
AGCTTCACCA CTCTGTTTCA AAACCAACAG TGTAGACTGG AAAAGCTGAA GTTCAGCAGC 
ATGTGTATTA CAGCAGAAGG TTGTGCTGCT TTGGCTTCAG CATTTAACTC AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGAGAAT AAACTAGGAG ACTCAGGAGT AACAGAAATC 
TCCAGTCTAC TGGCAAACTC GCAGTGCATG CTGAAGATAC TCGGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GACGCCTGTC GGTTTGTGAC TAAAATTGTG GGTAAAAACC CATTACTCCT GACAGAACTG 
AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAGAGTCAA CACAATAATG CTGAATAATA ACAGGATTAC AGCAGAAGGT 
TGTGCTGCTT TAACTTCAGC ATTTATTTTA AACCCATCAC ACCTGATAGA GCTCAATCTG 
AGTGGGAATA AACTAGGAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATACA 
CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGAAG CTCACTGATT TACTGCACAG TAGAAGCTGT 
AAATTCAGAT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GTGACTATCT TACTAAAGTT 
CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT 
CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GAAGAAAATC 
AAGCTGAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC 
TCCAAAACCA TCCTGAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
AGAGAAATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAAATACT CAGTCTTTCA 
GACTGCCGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGACT TACTGGAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG AAGGCTGTCA CTTTGTGACT GAAATGGTGG GTAAAAACCC ATTACTCCTG 
AAAGAACTGA ATCTGAGTGA TCGTGTACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT 
CTGCTGCAGG ATAAACACTG TCAGATCCAC ACACTCATTC TGTGTTATTG CAGTATTACA 
GAGGAACAGT GTGTGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA 
CTGAACCTCA GTGGAAACAA TCTAGGAGAC TCTGCAGTGA AAAAACTCAG TGATTTACTG 
ATGAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTG ATTGCAGTAT TACAGAGAAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTTATGGAC TTGGCCATCT GCTGAGCAGT 
GTGGATTGCA AGCTGAATAA ACTACATCTG TGTGATTGCA GTATTACAGG GAAACAGTTG 
CACTTCCTGA TTGCAGTTAT GTGTATGGAC CCATCACACC TGAGGGTACT GGACCTCAGT 
AAGAATCAAA TAAAAAGCAG AGGAATGAAC ATGCTGTGTG ATGTGCTGAA TAAACCACGC 
TGTAAACTCA AGAGTTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACACTTAACT 
CAGTCTTTGG CTGAGAACAA AGCACTGGAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT 
CAAATGGGAG ACTCCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA 
CTGAGGTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471028.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326703.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X9, mRNA.

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CloneID	ODa146931
Clone ID Related Accession (Same CDS sequence)	XM_021471028.1
Accession Version	XM_021471028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein NLRC5 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGATTTACAG CAGGATCCAC ACTGTACACT GAAGATACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGCC TGTCAGTTTT TGACTGGAGT TGTGGGTAAA 
AACCTGTTAC TCTTGAAAGA GCTGAATCTG AGTGGACATG AACTAGGACT CTCAGACAGC 
TTTCAAATGC TTGTTCTTTT ACTGCATGAT AAACACTGTA CGCTCAACAC ACTGATTCTT 
TCAGATTGCA GTATCACAGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GACATCAAAC 
CCATCATACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAACTCACTG ATTTACTACT GGATGAACGC TGTAGCTTAA AAATCATCAG GTTTCTGAAA 
AGTCCTGCTG CACAAGAGTC ATGTGATTTT CTCACTGAAG TTCTGGATGT AAATCCATTA 
TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAAATC 
TCTGCTTTTT TGATGGACCC TCATAGCAAA GTGGAGAAAA TCAAGCTGAA TAATTGTAAG 
CTGCTTGAGA AAAGCTCATC AGTTTTAGCA ACAGTTCTGT CTTCCAAAAC CATCCTGAAA 
GAGCTGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAGAT CTGTGAGGGA 
CTGAAGAATC CTGTGTGTGA GCTGAAGATA CTCAAGCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA GTTACTGCAG 
GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCATTACTTC TGAAAGAGCT AAATCTGAGT 
GATCGTGAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGTAAACTCA ACACACTCAT TCTTAGTAAA TGTGGTGTAA CAGTGGAAAG CTGTTCAGCT 
CTGGCTACAG TCCTGAGATC AGAGACCAGT CTAAAAGAGC TTGACATGAG CAACAATAAT 
CTGCAGGAAT CAGGAGTGAA GAAACTCCAG AGCGGATTAA AAAACACAAA CTGCACACTG 
GAAAAACTCA GTCTTTTAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGAAGCT CACTGAGTTA CTGAAACCAG ACTATACACT GAAGACACTG 
AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA 
AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG 
GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT 
AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA 
TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC 
TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG 
TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC 
AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC 
GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT 
CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT 
GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA 
TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA 
TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG 
GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG 
CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC 
AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA 
GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC 
TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT 
GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA 
GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG 
CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG 
GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG 
AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG 
GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC 
TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG 
AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT 
AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG 
TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA 
ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG 
AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326703.1
CDS	182..6973
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471028.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471028.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGATTTACAG CAGGATCCAC ACTGTACACT GAAGATACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGCC TGTCAGTTTT TGACTGGAGT TGTGGGTAAA 
AACCTGTTAC TCTTGAAAGA GCTGAATCTG AGTGGACATG AACTAGGACT CTCAGACAGC 
TTTCAAATGC TTGTTCTTTT ACTGCATGAT AAACACTGTA CGCTCAACAC ACTGATTCTT 
TCAGATTGCA GTATCACAGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GACATCAAAC 
CCATCATACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAACTCACTG ATTTACTACT GGATGAACGC TGTAGCTTAA AAATCATCAG GTTTCTGAAA 
AGTCCTGCTG CACAAGAGTC ATGTGATTTT CTCACTGAAG TTCTGGATGT AAATCCATTA 
TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAAATC 
TCTGCTTTTT TGATGGACCC TCATAGCAAA GTGGAGAAAA TCAAGCTGAA TAATTGTAAG 
CTGCTTGAGA AAAGCTCATC AGTTTTAGCA ACAGTTCTGT CTTCCAAAAC CATCCTGAAA 
GAGCTGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAGAT CTGTGAGGGA 
CTGAAGAATC CTGTGTGTGA GCTGAAGATA CTCAAGCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA GTTACTGCAG 
GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT 
CAGTTTGTGA CTGGAATTGT GGGTAAAAAC CCATTACTTC TGAAAGAGCT AAATCTGAGT 
GATCGTGAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGTAAACTCA ACACACTCAT TCTTAGTAAA TGTGGTGTAA CAGTGGAAAG CTGTTCAGCT 
CTGGCTACAG TCCTGAGATC AGAGACCAGT CTAAAAGAGC TTGACATGAG CAACAATAAT 
CTGCAGGAAT CAGGAGTGAA GAAACTCCAG AGCGGATTAA AAAACACAAA CTGCACACTG 
GAAAAACTCA GTCTTTTAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGAAGCT CACTGAGTTA CTGAAACCAG ACTATACACT GAAGACACTG 
AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA 
AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG 
GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT 
AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA 
TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC 
TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG 
TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC 
AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC 
GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT 
CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT 
GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA 
TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA 
TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG 
GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG 
CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC 
AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA 
GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC 
TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT 
GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA 
GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG 
CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG 
GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG 
AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG 
GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC 
TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG 
AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT 
AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG 
TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA 
ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG 
AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471029.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326704.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X10, mRNA.

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CloneID	ODa146932
Clone ID Related Accession (Same CDS sequence)	XM_021471029.1
Accession Version	XM_021471029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG 
ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA 
CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGTC TTTCAGACTG CAGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG 
CAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGACGCC 
TGTCGGTTTG TGACTAAAAT TGTGGGTAAA AACCCATTAC TCCTGACAGA ACTGAATCTG 
AGTGATCGTA AACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGTAGAG TCAACACAAT AATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT 
GCTTTAACTT CAGCATTTAT TTTAAACCCA TCACACCTGA TAGAGCTCAA TCTGAGTGGG 
AATAAACTAG GAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TACACAATGC 
CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GAAGCTCACT GATTTACTGC ACAGTAGAAG CTGTAAATTC 
AGATTTCTGA AGAGTTCTGC TGCACAGGAA GCATGTGACT ATCTTACTAA AGTTCTGGGT 
ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT 
GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGAAGAA AATCAAGCTG 
AATAATTGTG AGCTGACAGA GAAAAGCTGT TCACTTCTAG CTGCAGTTCT CTCCTCCAAA 
ACCATCCTGA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAGAA 
ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAAA TACTCAGTCT TTCAGACTGC 
CGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GACTTACTGG AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT 
GATGAAGGCT GTCACTTTGT GACTGAAATG GTGGGTAAAA ACCCATTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTGT ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTGCTG 
CAGGATAAAC ACTGTCAGAT CCACACACTC ATTCTGTGTT ATTGCAGTAT TACAGAGGAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAGTGGAA ACAATCTAGG AGACTCTGCA GTGAAAAAAC TCAGTGATTT ACTGATGAAC 
CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTGATTGCA GTATTACAGA GAAACAGTGT 
GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT 
GGGAATAGAC TAGGAGACTC TGGAGCTTAT GGACTTGGCC ATCTGCTGAG CAGTGTGGAT 
TGCAAGCTGA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGGGAAACA GTTGCACTTC 
CTGATTGCAG TTATGTGTAT GGACCCATCA CACCTGAGGG TACTGGACCT CAGTAAGAAT 
CAAATAAAAA GCAGAGGAAT GAACATGCTG TGTGATGTGC TGAATAAACC ACGCTGTAAA 
CTCAAGAGTT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACACTT AACTCAGTCT 
TTGGCTGAGA ACAAAGCACT GGAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG 
GGAGACTCCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG 
TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326704.1
CDS	182..6970
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471029.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471029.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG 
ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA 
CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGTC TTTCAGACTG CAGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG 
CAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGACGCC 
TGTCGGTTTG TGACTAAAAT TGTGGGTAAA AACCCATTAC TCCTGACAGA ACTGAATCTG 
AGTGATCGTA AACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGTAGAG TCAACACAAT AATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT 
GCTTTAACTT CAGCATTTAT TTTAAACCCA TCACACCTGA TAGAGCTCAA TCTGAGTGGG 
AATAAACTAG GAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TACACAATGC 
CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GAAGCTCACT GATTTACTGC ACAGTAGAAG CTGTAAATTC 
AGATTTCTGA AGAGTTCTGC TGCACAGGAA GCATGTGACT ATCTTACTAA AGTTCTGGGT 
ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT 
GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGAAGAA AATCAAGCTG 
AATAATTGTG AGCTGACAGA GAAAAGCTGT TCACTTCTAG CTGCAGTTCT CTCCTCCAAA 
ACCATCCTGA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAGAA 
ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAAA TACTCAGTCT TTCAGACTGC 
CGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GACTTACTGG AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT 
GATGAAGGCT GTCACTTTGT GACTGAAATG GTGGGTAAAA ACCCATTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTGT ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTGCTG 
CAGGATAAAC ACTGTCAGAT CCACACACTC ATTCTGTGTT ATTGCAGTAT TACAGAGGAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAGTGGAA ACAATCTAGG AGACTCTGCA GTGAAAAAAC TCAGTGATTT ACTGATGAAC 
CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTGATTGCA GTATTACAGA GAAACAGTGT 
GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT 
GGGAATAGAC TAGGAGACTC TGGAGCTTAT GGACTTGGCC ATCTGCTGAG CAGTGTGGAT 
TGCAAGCTGA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGGGAAACA GTTGCACTTC 
CTGATTGCAG TTATGTGTAT GGACCCATCA CACCTGAGGG TACTGGACCT CAGTAAGAAT 
CAAATAAAAA GCAGAGGAAT GAACATGCTG TGTGATGTGC TGAATAAACC ACGCTGTAAA 
CTCAAGAGTT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACACTT AACTCAGTCT 
TTGGCTGAGA ACAAAGCACT GGAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG 
GGAGACTCCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG 
TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471030.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326705.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X11, mRNA.

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CloneID	ODa146933
Clone ID Related Accession (Same CDS sequence)	XM_021471030.1
Accession Version	XM_021471030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG 
ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA 
CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GATTTACTGC AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT 
GATGACGCCT GTCGGTTTGT GACTAAAATT GTGGGTAAAA ACCCATTACT CCTGACAGAA 
CTGAATCTGA GTGATCGTAA ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTGTAGAGT CAACACAATA ATGCTGAATA ATAACAGGAT TACAGCAGAA 
GGTTGTGCTG CTTTAACTTC AGCATTTATT TTAAACCCAT CACACCTGAT AGAGCTCAAT 
CTGAGTGGGA ATAAACTAGG AGACTCAGGA GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT 
ACACAATGCC GATTAGAGAA ACTGAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG AAGCTCACTG ATTTACTGCA CAGTAGAAGC 
TGTAAATTCA GATTTCTGAA GAGTTCTGCT GCACAGGAAG CATGTGACTA TCTTACTAAA 
GTTCTGGGTA TAAGTCCATT ATTACTGACA GAACTGGATC TGAGTGAAGA TAAATTAGGA 
GATCTGGATG GAGAGAAACT CTCTGCTCTT CTGATGGACT CTCATAGCAA AGTGAAGAAA 
ATCAAGCTGA ATAATTGTGA GCTGACAGAG AAAAGCTGTT CACTTCTAGC TGCAGTTCTC 
TCCTCCAAAA CCATCCTGAA AGAGATGAAC CTGAACAACA GTCGTCTGCT GGACTCAGGA 
GTCAGAGAAA TCTGTGAGGG ACTGAAGAAT CCTGTGTGTG AGCTGAAAAT ACTCAGTCTT 
TCAGACTGCC GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ACTTACTGGA GGATCCACAC TGTACACTGA AGACACTGAG GTTTTTGAGT 
CCTGCTGCTG ATGAAGGCTG TCACTTTGTG ACTGAAATGG TGGGTAAAAA CCCATTACTC 
CTGAAAGAAC TGAATCTGAG TGATCGTGTA CTAGGAGACA CACGAGTGAA TCAACTCTCT 
GCTCTGCTGC AGGATAAACA CTGTCAGATC CACACACTCA TTCTGTGTTA TTGCAGTATT 
ACAGAGGAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAGTGGAAA CAATCTAGGA GACTCTGCAG TGAAAAAACT CAGTGATTTA 
CTGATGAACC AACAATTCAA GCTGGAGAAA CTACGTCTGA GTGATTGCAG TATTACAGAG 
AAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAATG GGAATAGACT AGGAGACTCT GGAGCTTATG GACTTGGCCA TCTGCTGAGC 
AGTGTGGATT GCAAGCTGAA TAAACTACAT CTGTGTGATT GCAGTATTAC AGGGAAACAG 
TTGCACTTCC TGATTGCAGT TATGTGTATG GACCCATCAC ACCTGAGGGT ACTGGACCTC 
AGTAAGAATC AAATAAAAAG CAGAGGAATG AACATGCTGT GTGATGTGCT GAATAAACCA 
CGCTGTAAAC TCAAGAGTTT AAGACTAAAT GACTGTGGCA TTACAGATGT TACACACTTA 
ACTCAGTCTT TGGCTGAGAA CAAAGCACTG GAGTTCTTAA AAGAGCTTGA ACTGAGTAAA 
AATCAAATGG GAGACTCCGA AAAGCAAAAA CTCAGTGACC TGCTGCGAGA CTCAAACTGT 
AAACTGAGGT TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326705.1
CDS	182..6799
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471030.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471030.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG 
ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA 
CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GATTTACTGC AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT 
GATGACGCCT GTCGGTTTGT GACTAAAATT GTGGGTAAAA ACCCATTACT CCTGACAGAA 
CTGAATCTGA GTGATCGTAA ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTACTG 
CAGGATAAAC ACTGTAGAGT CAACACAATA ATGCTGAATA ATAACAGGAT TACAGCAGAA 
GGTTGTGCTG CTTTAACTTC AGCATTTATT TTAAACCCAT CACACCTGAT AGAGCTCAAT 
CTGAGTGGGA ATAAACTAGG AGACTCAGGA GTAGAGAAGA TCTGTCCTCT GCTGGAAAAT 
ACACAATGCC GATTAGAGAA ACTGAGTCTT TCAGACTGCA GTATCAGTGA AGAAGGTTAT 
AAAGCTCTGG CTTCAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG AAGCTCACTG ATTTACTGCA CAGTAGAAGC 
TGTAAATTCA GATTTCTGAA GAGTTCTGCT GCACAGGAAG CATGTGACTA TCTTACTAAA 
GTTCTGGGTA TAAGTCCATT ATTACTGACA GAACTGGATC TGAGTGAAGA TAAATTAGGA 
GATCTGGATG GAGAGAAACT CTCTGCTCTT CTGATGGACT CTCATAGCAA AGTGAAGAAA 
ATCAAGCTGA ATAATTGTGA GCTGACAGAG AAAAGCTGTT CACTTCTAGC TGCAGTTCTC 
TCCTCCAAAA CCATCCTGAA AGAGATGAAC CTGAACAACA GTCGTCTGCT GGACTCAGGA 
GTCAGAGAAA TCTGTGAGGG ACTGAAGAAT CCTGTGTGTG AGCTGAAAAT ACTCAGTCTT 
TCAGACTGCC GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ACTTACTGGA GGATCCACAC TGTACACTGA AGACACTGAG GTTTTTGAGT 
CCTGCTGCTG ATGAAGGCTG TCACTTTGTG ACTGAAATGG TGGGTAAAAA CCCATTACTC 
CTGAAAGAAC TGAATCTGAG TGATCGTGTA CTAGGAGACA CACGAGTGAA TCAACTCTCT 
GCTCTGCTGC AGGATAAACA CTGTCAGATC CACACACTCA TTCTGTGTTA TTGCAGTATT 
ACAGAGGAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAGTGGAAA CAATCTAGGA GACTCTGCAG TGAAAAAACT CAGTGATTTA 
CTGATGAACC AACAATTCAA GCTGGAGAAA CTACGTCTGA GTGATTGCAG TATTACAGAG 
AAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAATG GGAATAGACT AGGAGACTCT GGAGCTTATG GACTTGGCCA TCTGCTGAGC 
AGTGTGGATT GCAAGCTGAA TAAACTACAT CTGTGTGATT GCAGTATTAC AGGGAAACAG 
TTGCACTTCC TGATTGCAGT TATGTGTATG GACCCATCAC ACCTGAGGGT ACTGGACCTC 
AGTAAGAATC AAATAAAAAG CAGAGGAATG AACATGCTGT GTGATGTGCT GAATAAACCA 
CGCTGTAAAC TCAAGAGTTT AAGACTAAAT GACTGTGGCA TTACAGATGT TACACACTTA 
ACTCAGTCTT TGGCTGAGAA CAAAGCACTG GAGTTCTTAA AAGAGCTTGA ACTGAGTAAA 
AATCAAATGG GAGACTCCGA AAAGCAAAAA CTCAGTGACC TGCTGCGAGA CTCAAACTGT 
AAACTGAGGT TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471031.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326706.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X12, mRNA.

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CloneID	ODa146934
Clone ID Related Accession (Same CDS sequence)	XM_021471031.1
Accession Version	XM_021471031.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ATTTACTGCA GGATCCACAC TGTACACTGA AGACACTGAG GTTTTTGAGT 
CCTGCTGCTG ATGACGCCTG TCGGTTTGTG ACTAAAATTG TGGGTAAAAA CCCATTACTC 
CTGACAGAAC TGAATCTGAG TGATCGTAAA CTAGGAGACA CACGAGTGAA TCAACTCTCT 
GCTCTACTGC AGGATAAACA CTGTAGAGTC AACACAATAA TGCTGAATAA TAACAGGATT 
ACAGCAGAAG GTTGTGCTGC TTTAACTTCA GCATTTATTT TAAACCCATC ACACCTGATA 
GAGCTCAATC TGAGTGGGAA TAAACTAGGA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG 
CTGGAAAATA CACAATGCCG ATTAGAGAAA CTGAGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGA AGCTCACTGA TTTACTGCAC 
AGTAGAAGCT GTAAATTCAG ATTTCTGAAG AGTTCTGCTG CACAGGAAGC ATGTGACTAT 
CTTACTAAAG TTCTGGGTAT AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT 
AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGCAAA 
GTGAAGAAAA TCAAGCTGAA TAATTGTGAG CTGACAGAGA AAAGCTGTTC ACTTCTAGCT 
GCAGTTCTCT CCTCCAAAAC CATCCTGAAA GAGATGAACC TGAACAACAG TCGTCTGCTG 
GACTCAGGAG TCAGAGAAAT CTGTGAGGGA CTGAAGAATC CTGTGTGTGA GCTGAAAATA 
CTCAGTCTTT CAGACTGCCG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA CTTACTGGAG GATCCACACT GTACACTGAA GACACTGAGG 
TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT CACTTTGTGA CTGAAATGGT GGGTAAAAAC 
CCATTACTCC TGAAAGAACT GAATCTGAGT GATCGTGTAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTCAGATCC ACACACTCAT TCTGTGTTAT 
TGCAGTATTA CAGAGGAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGAACCT CAGTGGAAAC AATCTAGGAG ACTCTGCAGT GAAAAAACTC 
AGTGATTTAC TGATGAACCA ACAATTCAAG CTGGAGAAAC TACGTCTGAG TGATTGCAGT 
ATTACAGAGA AACAGTGTGT GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG 
AGAGAACTGA ACCTCAATGG GAATAGACTA GGAGACTCTG GAGCTTATGG ACTTGGCCAT 
CTGCTGAGCA GTGTGGATTG CAAGCTGAAT AAACTACATC TGTGTGATTG CAGTATTACA 
GGGAAACAGT TGCACTTCCT GATTGCAGTT ATGTGTATGG ACCCATCACA CCTGAGGGTA 
CTGGACCTCA GTAAGAATCA AATAAAAAGC AGAGGAATGA ACATGCTGTG TGATGTGCTG 
AATAAACCAC GCTGTAAACT CAAGAGTTTA AGACTAAATG ACTGTGGCAT TACAGATGTT 
ACACACTTAA CTCAGTCTTT GGCTGAGAAC AAAGCACTGG AGTTCTTAAA AGAGCTTGAA 
CTGAGTAAAA ATCAAATGGG AGACTCCGAA AAGCAAAAAC TCAGTGACCT GCTGCGAGAC 
TCAAACTGTA AACTGAGGTT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326706.1
CDS	182..6628
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471031.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471031.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
TCAGACTGCA GTATCAGTGA AGAAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CCTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ATTTACTGCA GGATCCACAC TGTACACTGA AGACACTGAG GTTTTTGAGT 
CCTGCTGCTG ATGACGCCTG TCGGTTTGTG ACTAAAATTG TGGGTAAAAA CCCATTACTC 
CTGACAGAAC TGAATCTGAG TGATCGTAAA CTAGGAGACA CACGAGTGAA TCAACTCTCT 
GCTCTACTGC AGGATAAACA CTGTAGAGTC AACACAATAA TGCTGAATAA TAACAGGATT 
ACAGCAGAAG GTTGTGCTGC TTTAACTTCA GCATTTATTT TAAACCCATC ACACCTGATA 
GAGCTCAATC TGAGTGGGAA TAAACTAGGA GACTCAGGAG TAGAGAAGAT CTGTCCTCTG 
CTGGAAAATA CACAATGCCG ATTAGAGAAA CTGAGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGA AGCTCACTGA TTTACTGCAC 
AGTAGAAGCT GTAAATTCAG ATTTCTGAAG AGTTCTGCTG CACAGGAAGC ATGTGACTAT 
CTTACTAAAG TTCTGGGTAT AAGTCCATTA TTACTGACAG AACTGGATCT GAGTGAAGAT 
AAATTAGGAG ATCTGGATGG AGAGAAACTC TCTGCTCTTC TGATGGACTC TCATAGCAAA 
GTGAAGAAAA TCAAGCTGAA TAATTGTGAG CTGACAGAGA AAAGCTGTTC ACTTCTAGCT 
GCAGTTCTCT CCTCCAAAAC CATCCTGAAA GAGATGAACC TGAACAACAG TCGTCTGCTG 
GACTCAGGAG TCAGAGAAAT CTGTGAGGGA CTGAAGAATC CTGTGTGTGA GCTGAAAATA 
CTCAGTCTTT CAGACTGCCG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA CTTACTGGAG GATCCACACT GTACACTGAA GACACTGAGG 
TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT CACTTTGTGA CTGAAATGGT GGGTAAAAAC 
CCATTACTCC TGAAAGAACT GAATCTGAGT GATCGTGTAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTCAGATCC ACACACTCAT TCTGTGTTAT 
TGCAGTATTA CAGAGGAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGAACCT CAGTGGAAAC AATCTAGGAG ACTCTGCAGT GAAAAAACTC 
AGTGATTTAC TGATGAACCA ACAATTCAAG CTGGAGAAAC TACGTCTGAG TGATTGCAGT 
ATTACAGAGA AACAGTGTGT GATCCTGACT TCAGCTCTGA AATCAAACCC ATCACACCTG 
AGAGAACTGA ACCTCAATGG GAATAGACTA GGAGACTCTG GAGCTTATGG ACTTGGCCAT 
CTGCTGAGCA GTGTGGATTG CAAGCTGAAT AAACTACATC TGTGTGATTG CAGTATTACA 
GGGAAACAGT TGCACTTCCT GATTGCAGTT ATGTGTATGG ACCCATCACA CCTGAGGGTA 
CTGGACCTCA GTAAGAATCA AATAAAAAGC AGAGGAATGA ACATGCTGTG TGATGTGCTG 
AATAAACCAC GCTGTAAACT CAAGAGTTTA AGACTAAATG ACTGTGGCAT TACAGATGTT 
ACACACTTAA CTCAGTCTTT GGCTGAGAAC AAAGCACTGG AGTTCTTAAA AGAGCTTGAA 
CTGAGTAAAA ATCAAATGGG AGACTCCGAA AAGCAAAAAC TCAGTGACCT GCTGCGAGAC 
TCAAACTGTA AACTGAGGTT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471032.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326707.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X13, mRNA.

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CloneID	ODa146935
Clone ID Related Accession (Same CDS sequence)	XM_021471032.1
Accession Version	XM_021471032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG 
ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA 
CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT 
GAAGCCTGTA CACATCTGAA TAAAGTTCTG GGTGGAAACC TGTTACTCTT GAGAGAGCTG 
AATCTGAGTA AACTTGATCT TGGACCATCA GGATTGGAGA AACTTGCTGC TGTACTGAAA 
GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCAGTATGTC AGAAGAAGAT 
TGTCGTGTTT TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGACAGA GCTGAATTTG 
AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CCACTCTGTT TCAAAACCAA 
CAGTGTAGAC TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT 
GCTTTGGCTT CAGCATTTAA CTCAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGAG 
AATAAACTAG GAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCGCAGTGC 
ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGGATCCACA CTGTACACTG 
AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG 
GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC 
ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA 
GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT 
GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA 
CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG 
TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326707.1
CDS	182..6460
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471032.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471032.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG 
ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA 
CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTTAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GAGTTACTGA AACCAGACTA TACACTGAAG ACACTGAGGT TTTTGAGTGA TGATGCAGAT 
GAAGCCTGTA CACATCTGAA TAAAGTTCTG GGTGGAAACC TGTTACTCTT GAGAGAGCTG 
AATCTGAGTA AACTTGATCT TGGACCATCA GGATTGGAGA AACTTGCTGC TGTACTGAAA 
GATAAACACT GTAAACTCAA CACACTGATT CTGAATAACA GCAGTATGTC AGAAGAAGAT 
TGTCGTGTTT TGGCTGAAGC TTTTAATTCA AACCCATCAA ATCTGACAGA GCTGAATTTG 
AGTGGGACTA AACTCACAAA CTCAGGAATG AAGAGCTTCA CCACTCTGTT TCAAAACCAA 
CAGTGTAGAC TGGAAAAGCT GAAGTTCAGC AGCATGTGTA TTACAGCAGA AGGTTGTGCT 
GCTTTGGCTT CAGCATTTAA CTCAAATCCT TCACACCTGA TAGAGCTCAA TCTGAGTGAG 
AATAAACTAG GAGACTCAGG AGTAACAGAA ATCTCCAGTC TACTGGCAAA CTCGCAGTGC 
ATGCTGAAGA TACTCGGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GATTTACTGC AGGATCCACA CTGTACACTG 
AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG 
GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC 
ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA 
GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT 
GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA 
CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG 
TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471033.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326708.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X14, mRNA.

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CloneID	ODa146936
Clone ID Related Accession (Same CDS sequence)	XM_021471033.1
Accession Version	XM_021471033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATGCTG 
AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTAA CTTCAGCATT TATTTTAAAC 
CCATCACACC TGATAGAGCT CAATCTGAGT GGGAATAAAC TAGGAGACTC AGGAGTAGAG 
AAGATCTGTC CTCTGCTGGA AAATACACAA TGCCGATTAG AGAAACTGAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGAAGCTC 
ACTGATTTAC TGCACAGTAG AAGCTGTAAA TTCAGATTTC TGAAGAGTTC TGCTGCACAG 
GAAGCATGTG ACTATCTTAC TAAAGTTCTG GGTATAAGTC CATTATTACT GACAGAACTG 
GATCTGAGTG AAGATAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGATG 
GACTCTCATA GCAAAGTGAA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCACTTC TAGCTGCAGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAAATCTGTG AGGGACTGAA GAATCCTGTG 
TGTGAGCTGA AAATACTCAG TCTTTCAGAC TGCCGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGGAGCTC ACTGACTTAC TGGAGGATCC ACACTGTACA 
CTGAAGACAC TGAGGTTTTT GAGTCCTGCT GCTGATGAAG GCTGTCACTT TGTGACTGAA 
ATGGTGGGTA AAAACCCATT ACTCCTGAAA GAACTGAATC TGAGTGATCG TGTACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTG CTGCAGGATA AACACTGTCA GATCCACACA 
CTCATTCTGT GTTATTGCAG TATTACAGAG GAACAGTGTG TGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAGTG GAAACAATCT AGGAGACTCT 
GCAGTGAAAA AACTCAGTGA TTTACTGATG AACCAACAAT TCAAGCTGGA GAAACTACGT 
CTGAGTGATT GCAGTATTAC AGAGAAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AATGGGAATA GACTAGGAGA CTCTGGAGCT 
TATGGACTTG GCCATCTGCT GAGCAGTGTG GATTGCAAGC TGAATAAACT ACATCTGTGT 
GATTGCAGTA TTACAGGGAA ACAGTTGCAC TTCCTGATTG CAGTTATGTG TATGGACCCA 
TCACACCTGA GGGTACTGGA CCTCAGTAAG AATCAAATAA AAAGCAGAGG AATGAACATG 
CTGTGTGATG TGCTGAATAA ACCACGCTGT AAACTCAAGA GTTTAAGACT AAATGACTGT 
GGCATTACAG ATGTTACACA CTTAACTCAG TCTTTGGCTG AGAACAAAGC ACTGGAGTTC 
TTAAAAGAGC TTGAACTGAG TAAAAATCAA ATGGGAGACT CCGAAAAGCA AAAACTCAGT 
GACCTGCTGC GAGACTCAAA CTGTAAACTG AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326708.1
CDS	182..6283
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471033.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471033.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATGCTG 
AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTAA CTTCAGCATT TATTTTAAAC 
CCATCACACC TGATAGAGCT CAATCTGAGT GGGAATAAAC TAGGAGACTC AGGAGTAGAG 
AAGATCTGTC CTCTGCTGGA AAATACACAA TGCCGATTAG AGAAACTGAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGAAGCTC 
ACTGATTTAC TGCACAGTAG AAGCTGTAAA TTCAGATTTC TGAAGAGTTC TGCTGCACAG 
GAAGCATGTG ACTATCTTAC TAAAGTTCTG GGTATAAGTC CATTATTACT GACAGAACTG 
GATCTGAGTG AAGATAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGATG 
GACTCTCATA GCAAAGTGAA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCACTTC TAGCTGCAGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAAATCTGTG AGGGACTGAA GAATCCTGTG 
TGTGAGCTGA AAATACTCAG TCTTTCAGAC TGCCGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGGAGCTC ACTGACTTAC TGGAGGATCC ACACTGTACA 
CTGAAGACAC TGAGGTTTTT GAGTCCTGCT GCTGATGAAG GCTGTCACTT TGTGACTGAA 
ATGGTGGGTA AAAACCCATT ACTCCTGAAA GAACTGAATC TGAGTGATCG TGTACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTG CTGCAGGATA AACACTGTCA GATCCACACA 
CTCATTCTGT GTTATTGCAG TATTACAGAG GAACAGTGTG TGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAGTG GAAACAATCT AGGAGACTCT 
GCAGTGAAAA AACTCAGTGA TTTACTGATG AACCAACAAT TCAAGCTGGA GAAACTACGT 
CTGAGTGATT GCAGTATTAC AGAGAAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AATGGGAATA GACTAGGAGA CTCTGGAGCT 
TATGGACTTG GCCATCTGCT GAGCAGTGTG GATTGCAAGC TGAATAAACT ACATCTGTGT 
GATTGCAGTA TTACAGGGAA ACAGTTGCAC TTCCTGATTG CAGTTATGTG TATGGACCCA 
TCACACCTGA GGGTACTGGA CCTCAGTAAG AATCAAATAA AAAGCAGAGG AATGAACATG 
CTGTGTGATG TGCTGAATAA ACCACGCTGT AAACTCAAGA GTTTAAGACT AAATGACTGT 
GGCATTACAG ATGTTACACA CTTAACTCAG TCTTTGGCTG AGAACAAAGC ACTGGAGTTC 
TTAAAAGAGC TTGAACTGAG TAAAAATCAA ATGGGAGACT CCGAAAAGCA AAAACTCAGT 
GACCTGCTGC GAGACTCAAA CTGTAAACTG AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471034.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326709.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X15, mRNA.

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CloneID	ODa146937
Clone ID Related Accession (Same CDS sequence)	XM_021471034.1
Accession Version	XM_021471034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA ATCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGATTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAC GCCTGTCGGT TTGTGACTAA AATTGTGGGT 
AAAAACCCAT TACTCCTGAC AGAACTGAAT CTGAGTGATC GTAAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA GAGTCAACAC AATAATGCTG 
AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTAA CTTCAGCATT TATTTTAAAC 
CCATCACACC TGATAGAGCT CAATCTGAGT GGGAATAAAC TAGGAGACTC AGGAGTAGAG 
AAGATCTGTC CTCTGCTGGA AAATACACAA TGCCGATTAG AGAAACTGAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGAAGCTC 
ACTGATTTAC TGCACAGTAG AAGCTGTAAA TTCAGATTTC TGAAGAGTTC TGCTGCACAG 
GAAGCATGTG ACTATCTTAC TAAAGTTCTG GGTATAAGTC CATTATTACT GACAGAACTG 
GATCTGAGTG AAGATAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGATG 
GACTCTCATA GCAAAGTGAA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCACTTC TAGCTGCAGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAAATCTGTG AGGGACTGAA GAATCCTGTG 
TGTGAGCTGA AAATACTCAG TCTTTCAGAC TGCCGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGGAGCTC ACTGACTTAC TGGAGGATCC ACACTGTACA 
CTGAAGACAC TGAGGTTTTT GAGTCCTGCT GCTGATGAAG GCTGTCACTT TGTGACTGAA 
ATGGTGGGTA AAAACCCATT ACTCCTGAAA GAACTGAATC TGAGTGATCG TGTACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTG CTGCAGGATA AACACTGTCA GATCCACACA 
CTCATTCTGT GTTATTGCAG TATTACAGAG GAACAGTGTG TGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAGTG GAAACAATCT AGGAGACTCT 
GCAGTGAAAA AACTCAGTGA TTTACTGATG AACCAACAAT TCAAGCTGGA GAAACTACGT 
CTGAGTGATT GCAGTATTAC AGAGAAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AATGGGAATA GACTAGGAGA CTCTGGAGCT 
TATGGACTTG GCCATCTGCT GAGCAGTGTG GATTGCAAGC TGAATAAACT ACATCTGTGT 
GATTGCAGTA TTACAGGGAA ACAGTTGCAC TTCCTGATTG CAGTTATGTG TATGGACCCA 
TCACACCTGA GGGTACTGGA CCTCAGTAAG AATCAAATAA AAAGCAGAGG AATGAACATG 
CTGTGTGATG TGCTGAATAA ACCACGCTGT AAACTCAAGA GTTTAAGACT AAATGACTGT 
GGCATTACAG ATGTTACACA CTTAACTCAG TCTTTGGCTG AGAACAAAGC ACTGGAGTTC 
TTAAAAGAGC TTGAACTGAG TAAAAATCAA ATGGGAGACT CCGAAAAGCA AAAACTCAGT 
GACCTGCTGC GAGACTCAAA CTGTAAACTG AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326709.1
CDS	182..6283
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471034.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471034.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA ATCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGATTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAC GCCTGTCGGT TTGTGACTAA AATTGTGGGT 
AAAAACCCAT TACTCCTGAC AGAACTGAAT CTGAGTGATC GTAAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA GAGTCAACAC AATAATGCTG 
AATAATAACA GGATTACAGC AGAAGGTTGT GCTGCTTTAA CTTCAGCATT TATTTTAAAC 
CCATCACACC TGATAGAGCT CAATCTGAGT GGGAATAAAC TAGGAGACTC AGGAGTAGAG 
AAGATCTGTC CTCTGCTGGA AAATACACAA TGCCGATTAG AGAAACTGAG TCTTTCAGAC 
TGCAGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGAAGCTC 
ACTGATTTAC TGCACAGTAG AAGCTGTAAA TTCAGATTTC TGAAGAGTTC TGCTGCACAG 
GAAGCATGTG ACTATCTTAC TAAAGTTCTG GGTATAAGTC CATTATTACT GACAGAACTG 
GATCTGAGTG AAGATAAATT AGGAGATCTG GATGGAGAGA AACTCTCTGC TCTTCTGATG 
GACTCTCATA GCAAAGTGAA GAAAATCAAG CTGAATAATT GTGAGCTGAC AGAGAAAAGC 
TGTTCACTTC TAGCTGCAGT TCTCTCCTCC AAAACCATCC TGAAAGAGAT GAACCTGAAC 
AACAGTCGTC TGCTGGACTC AGGAGTCAGA GAAATCTGTG AGGGACTGAA GAATCCTGTG 
TGTGAGCTGA AAATACTCAG TCTTTCAGAC TGCCGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGGAGCTC ACTGACTTAC TGGAGGATCC ACACTGTACA 
CTGAAGACAC TGAGGTTTTT GAGTCCTGCT GCTGATGAAG GCTGTCACTT TGTGACTGAA 
ATGGTGGGTA AAAACCCATT ACTCCTGAAA GAACTGAATC TGAGTGATCG TGTACTAGGA 
GACACACGAG TGAATCAACT CTCTGCTCTG CTGCAGGATA AACACTGTCA GATCCACACA 
CTCATTCTGT GTTATTGCAG TATTACAGAG GAACAGTGTG TGATCCTGAC TTCAGCTCTG 
AAATCAAACC CATCACACCT GAGAGAACTG AACCTCAGTG GAAACAATCT AGGAGACTCT 
GCAGTGAAAA AACTCAGTGA TTTACTGATG AACCAACAAT TCAAGCTGGA GAAACTACGT 
CTGAGTGATT GCAGTATTAC AGAGAAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AATGGGAATA GACTAGGAGA CTCTGGAGCT 
TATGGACTTG GCCATCTGCT GAGCAGTGTG GATTGCAAGC TGAATAAACT ACATCTGTGT 
GATTGCAGTA TTACAGGGAA ACAGTTGCAC TTCCTGATTG CAGTTATGTG TATGGACCCA 
TCACACCTGA GGGTACTGGA CCTCAGTAAG AATCAAATAA AAAGCAGAGG AATGAACATG 
CTGTGTGATG TGCTGAATAA ACCACGCTGT AAACTCAAGA GTTTAAGACT AAATGACTGT 
GGCATTACAG ATGTTACACA CTTAACTCAG TCTTTGGCTG AGAACAAAGC ACTGGAGTTC 
TTAAAAGAGC TTGAACTGAG TAAAAATCAA ATGGGAGACT CCGAAAAGCA AAAACTCAGT 
GACCTGCTGC GAGACTCAAA CTGTAAACTG AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471035.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326710.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X16, mRNA.

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CloneID	ODa146938
Clone ID Related Accession (Same CDS sequence)	XM_021471035.1
Accession Version	XM_021471035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG 
ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA 
CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG 
AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG 
GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC 
ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA 
GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT 
GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA 
CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG 
TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326710.1
CDS	182..6280
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471035.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471035.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTTTCAGA CTGCAGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGAGTTA CTGCAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCAGT TTGTGACTGG AATTGTGGGT 
AAAAACCCAT TACTTCTGAA AGAGCTAAAT CTGAGTGATC GTGAACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATTCTT 
AGTAAATGTG GTGTAACAGT GGAAAGCTGT TCAGCTCTGG CTACAGTCCT GAGATCAGAG 
ACCAGTCTAA AAGAGCTTGA CATGAGCAAC AATAATCTGC AGGAATCAGG AGTGAAGAAA 
CTCCAGAGCG GATTAAAAAA CACAAACTGC ACACTGGAAA AACTCAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG 
AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG 
GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC 
ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA 
GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT 
GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA 
CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG 
TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471036.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326711.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X17, mRNA.

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CloneID	ODa146939
Clone ID Related Accession (Same CDS sequence)	XM_021471036.1
Accession Version	XM_021471036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTAACTTCA 
GCATTTATTT TAAACCCATC ACACCTGATA GAGCTCAATC TGAGTGGGAA TAAACTAGGA 
GACTCAGGAG TAGAGAAGAT CTGTCCTCTG CTGGAAAATA CACAATGCCG ATTAGAGAAA 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGA AGCTCACTGA TTTACTGCAC AGTAGAAGCT GTAAATTCAG ATTTCTGAAG 
AGTTCTGCTG CACAGGAAGC ATGTGACTAT CTTACTAAAG TTCTGGGTAT AAGTCCATTA 
TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAACTC 
TCTGCTCTTC TGATGGACTC TCATAGCAAA GTGAAGAAAA TCAAGCTGAA TAATTGTGAG 
CTGACAGAGA AAAGCTGTTC ACTTCTAGCT GCAGTTCTCT CCTCCAAAAC CATCCTGAAA 
GAGATGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAAAT CTGTGAGGGA 
CTGAAGAATC CTGTGTGTGA GCTGAAAATA CTCAGTCTTT CAGACTGCCG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA CTTACTGGAG 
GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT 
CACTTTGTGA CTGAAATGGT GGGTAAAAAC CCATTACTCC TGAAAGAACT GAATCTGAGT 
GATCGTGTAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTGCTGCA GGATAAACAC 
TGTCAGATCC ACACACTCAT TCTGTGTTAT TGCAGTATTA CAGAGGAACA GTGTGTGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAGTGGAAAC 
AATCTAGGAG ACTCTGCAGT GAAAAAACTC AGTGATTTAC TGATGAACCA ACAATTCAAG 
CTGGAGAAAC TACGTCTGAG TGATTGCAGT ATTACAGAGA AACAGTGTGT GATCCTGACT 
TCAGCTCTGA AATCAAACCC ATCACACCTG AGAGAACTGA ACCTCAATGG GAATAGACTA 
GGAGACTCTG GAGCTTATGG ACTTGGCCAT CTGCTGAGCA GTGTGGATTG CAAGCTGAAT 
AAACTACATC TGTGTGATTG CAGTATTACA GGGAAACAGT TGCACTTCCT GATTGCAGTT 
ATGTGTATGG ACCCATCACA CCTGAGGGTA CTGGACCTCA GTAAGAATCA AATAAAAAGC 
AGAGGAATGA ACATGCTGTG TGATGTGCTG AATAAACCAC GCTGTAAACT CAAGAGTTTA 
AGACTAAATG ACTGTGGCAT TACAGATGTT ACACACTTAA CTCAGTCTTT GGCTGAGAAC 
AAAGCACTGG AGTTCTTAAA AGAGCTTGAA CTGAGTAAAA ATCAAATGGG AGACTCCGAA 
AAGCAAAAAC TCAGTGACCT GCTGCGAGAC TCAAACTGTA AACTGAGGTT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326711.1
CDS	182..5938
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471036.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471036.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTAAGCTGC TTGAGAAAAG CTCATCAGTT 
TTAGCAACAG TTCTGTCTTC CAAAACCATC CTGAAAGAGC TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAGATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAGATACTCA AGCTGAATAA TAACAGGATT ACAGCAGAAG GTTGTGCTGC TTTAACTTCA 
GCATTTATTT TAAACCCATC ACACCTGATA GAGCTCAATC TGAGTGGGAA TAAACTAGGA 
GACTCAGGAG TAGAGAAGAT CTGTCCTCTG CTGGAAAATA CACAATGCCG ATTAGAGAAA 
CTGAGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGA AGCTCACTGA TTTACTGCAC AGTAGAAGCT GTAAATTCAG ATTTCTGAAG 
AGTTCTGCTG CACAGGAAGC ATGTGACTAT CTTACTAAAG TTCTGGGTAT AAGTCCATTA 
TTACTGACAG AACTGGATCT GAGTGAAGAT AAATTAGGAG ATCTGGATGG AGAGAAACTC 
TCTGCTCTTC TGATGGACTC TCATAGCAAA GTGAAGAAAA TCAAGCTGAA TAATTGTGAG 
CTGACAGAGA AAAGCTGTTC ACTTCTAGCT GCAGTTCTCT CCTCCAAAAC CATCCTGAAA 
GAGATGAACC TGAACAACAG TCGTCTGCTG GACTCAGGAG TCAGAGAAAT CTGTGAGGGA 
CTGAAGAATC CTGTGTGTGA GCTGAAAATA CTCAGTCTTT CAGACTGCCG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA CTTACTGGAG 
GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGAAGGCTGT 
CACTTTGTGA CTGAAATGGT GGGTAAAAAC CCATTACTCC TGAAAGAACT GAATCTGAGT 
GATCGTGTAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTGCTGCA GGATAAACAC 
TGTCAGATCC ACACACTCAT TCTGTGTTAT TGCAGTATTA CAGAGGAACA GTGTGTGATC 
CTGACTTCAG CTCTGAAATC AAACCCATCA CACCTGAGAG AACTGAACCT CAGTGGAAAC 
AATCTAGGAG ACTCTGCAGT GAAAAAACTC AGTGATTTAC TGATGAACCA ACAATTCAAG 
CTGGAGAAAC TACGTCTGAG TGATTGCAGT ATTACAGAGA AACAGTGTGT GATCCTGACT 
TCAGCTCTGA AATCAAACCC ATCACACCTG AGAGAACTGA ACCTCAATGG GAATAGACTA 
GGAGACTCTG GAGCTTATGG ACTTGGCCAT CTGCTGAGCA GTGTGGATTG CAAGCTGAAT 
AAACTACATC TGTGTGATTG CAGTATTACA GGGAAACAGT TGCACTTCCT GATTGCAGTT 
ATGTGTATGG ACCCATCACA CCTGAGGGTA CTGGACCTCA GTAAGAATCA AATAAAAAGC 
AGAGGAATGA ACATGCTGTG TGATGTGCTG AATAAACCAC GCTGTAAACT CAAGAGTTTA 
AGACTAAATG ACTGTGGCAT TACAGATGTT ACACACTTAA CTCAGTCTTT GGCTGAGAAC 
AAAGCACTGG AGTTCTTAAA AGAGCTTGAA CTGAGTAAAA ATCAAATGGG AGACTCCGAA 
AAGCAAAAAC TCAGTGACCT GCTGCGAGAC TCAAACTGTA AACTGAGGTT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471037.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326712.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X18, mRNA.

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CloneID	ODa146940
Clone ID Related Accession (Same CDS sequence)	XM_021471037.1
Accession Version	XM_021471037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NLR family CARD domain-containing protein 3 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTG ATGATGCAGA TGAAGCCTGT ACACATCTGA ATAAAGTTCT GGGTGGAAAC 
CTGTTACTCT TGAGAGAGCT GAATCTGAGT AAACTTGATC TTGGACCATC AGGATTGGAG 
AAACTTGCTG CTGTACTGAA AGATAAACAC TGTAAACTCA ACACACTGAT TCTGAATAAC 
AGCAGTATGT CAGAAGAAGA TTGTCGTGTT TTGGCTGAAG CTTTTAATTC AAACCCATCA 
AATCTGACAG AGCTGAATTT GAGTGGGACT AAACTCACAA ACTCAGGAAT GAAGAGCTTC 
ACCACTCTGT TTCAAAACCA ACAGTGTAGA CTGGAAAAGC TGAAGTTCAG CAGCATGTGT 
ATTACAGCAG AAGGTTGTGC TGCTTTGGCT TCAGCATTTA ACTCAAATCC TTCACACCTG 
ATAGAGCTCA ATCTGAGTGA GAATAAACTA GGAGACTCAG GAGTAACAGA AATCTCCAGT 
CTACTGGCAA ACTCGCAGTG CATGCTGAAG ATACTCGGTC TTTCAGACTG CAGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG 
CAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGACGCC 
TGTCGGTTTG TGACTAAAAT TGTGGGTAAA AACCCATTAC TCCTGACAGA ACTGAATCTG 
AGTGATCGTA AACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGTAGAG TCAACACAAT AATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT 
GCTTTAACTT CAGCATTTAT TTTAAACCCA TCACACCTGA TAGAGCTCAA TCTGAGTGGG 
AATAAACTAG GAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TACACAATGC 
CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GAAGCTCACT GATTTACTGC ACAGTAGAAG CTGTAAATTC 
AGATTTCTGA AGAGTTCTGC TGCACAGGAA GCATGTGACT ATCTTACTAA AGTTCTGGGT 
ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT 
GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGAAGAA AATCAAGCTG 
AATAATTGTG AGCTGACAGA GAAAAGCTGT TCACTTCTAG CTGCAGTTCT CTCCTCCAAA 
ACCATCCTGA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAGAA 
ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAAA TACTCAGTCT TTCAGACTGC 
CGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GACTTACTGG AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT 
GATGAAGGCT GTCACTTTGT GACTGAAATG GTGGGTAAAA ACCCATTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTGT ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTGCTG 
CAGGATAAAC ACTGTCAGAT CCACACACTC ATTCTGTGTT ATTGCAGTAT TACAGAGGAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAGTGGAA ACAATCTAGG AGACTCTGCA GTGAAAAAAC TCAGTGATTT ACTGATGAAC 
CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTGATTGCA GTATTACAGA GAAACAGTGT 
GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT 
GGGAATAGAC TAGGAGACTC TGGAGCTTAT GGACTTGGCC ATCTGCTGAG CAGTGTGGAT 
TGCAAGCTGA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGGGAAACA GTTGCACTTC 
CTGATTGCAG TTATGTGTAT GGACCCATCA CACCTGAGGG TACTGGACCT CAGTAAGAAT 
CAAATAAAAA GCAGAGGAAT GAACATGCTG TGTGATGTGC TGAATAAACC ACGCTGTAAA 
CTCAAGAGTT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACACTT AACTCAGTCT 
TTGGCTGAGA ACAAAGCACT GGAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG 
GGAGACTCCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG 
TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326712.1
CDS	182..5590
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471037.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471037.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTG ATGATGCAGA TGAAGCCTGT ACACATCTGA ATAAAGTTCT GGGTGGAAAC 
CTGTTACTCT TGAGAGAGCT GAATCTGAGT AAACTTGATC TTGGACCATC AGGATTGGAG 
AAACTTGCTG CTGTACTGAA AGATAAACAC TGTAAACTCA ACACACTGAT TCTGAATAAC 
AGCAGTATGT CAGAAGAAGA TTGTCGTGTT TTGGCTGAAG CTTTTAATTC AAACCCATCA 
AATCTGACAG AGCTGAATTT GAGTGGGACT AAACTCACAA ACTCAGGAAT GAAGAGCTTC 
ACCACTCTGT TTCAAAACCA ACAGTGTAGA CTGGAAAAGC TGAAGTTCAG CAGCATGTGT 
ATTACAGCAG AAGGTTGTGC TGCTTTGGCT TCAGCATTTA ACTCAAATCC TTCACACCTG 
ATAGAGCTCA ATCTGAGTGA GAATAAACTA GGAGACTCAG GAGTAACAGA AATCTCCAGT 
CTACTGGCAA ACTCGCAGTG CATGCTGAAG ATACTCGGTC TTTCAGACTG CAGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG 
CAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGACGCC 
TGTCGGTTTG TGACTAAAAT TGTGGGTAAA AACCCATTAC TCCTGACAGA ACTGAATCTG 
AGTGATCGTA AACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGTAGAG TCAACACAAT AATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT 
GCTTTAACTT CAGCATTTAT TTTAAACCCA TCACACCTGA TAGAGCTCAA TCTGAGTGGG 
AATAAACTAG GAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TACACAATGC 
CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GAAGCTCACT GATTTACTGC ACAGTAGAAG CTGTAAATTC 
AGATTTCTGA AGAGTTCTGC TGCACAGGAA GCATGTGACT ATCTTACTAA AGTTCTGGGT 
ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT 
GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGAAGAA AATCAAGCTG 
AATAATTGTG AGCTGACAGA GAAAAGCTGT TCACTTCTAG CTGCAGTTCT CTCCTCCAAA 
ACCATCCTGA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAGAA 
ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAAA TACTCAGTCT TTCAGACTGC 
CGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GACTTACTGG AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT 
GATGAAGGCT GTCACTTTGT GACTGAAATG GTGGGTAAAA ACCCATTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTGT ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTGCTG 
CAGGATAAAC ACTGTCAGAT CCACACACTC ATTCTGTGTT ATTGCAGTAT TACAGAGGAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAGTGGAA ACAATCTAGG AGACTCTGCA GTGAAAAAAC TCAGTGATTT ACTGATGAAC 
CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTGATTGCA GTATTACAGA GAAACAGTGT 
GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT 
GGGAATAGAC TAGGAGACTC TGGAGCTTAT GGACTTGGCC ATCTGCTGAG CAGTGTGGAT 
TGCAAGCTGA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGGGAAACA GTTGCACTTC 
CTGATTGCAG TTATGTGTAT GGACCCATCA CACCTGAGGG TACTGGACCT CAGTAAGAAT 
CAAATAAAAA GCAGAGGAAT GAACATGCTG TGTGATGTGC TGAATAAACC ACGCTGTAAA 
CTCAAGAGTT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACACTT AACTCAGTCT 
TTGGCTGAGA ACAAAGCACT GGAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG 
GGAGACTCCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG 
TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471038.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326713.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X19, mRNA.

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CloneID	ODa146941
Clone ID Related Accession (Same CDS sequence)	XM_021471038.1
Accession Version	XM_021471038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NLR family CARD domain-containing protein 3 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GACGCCTGTC GGTTTGTGAC TAAAATTGTG GGTAAAAACC CATTACTCCT GACAGAACTG 
AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAGAGTCAA CACAATAATG CTGAATAATA ACAGGATTAC AGCAGAAGGT 
TGTGCTGCTT TAACTTCAGC ATTTATTTTA AACCCATCAC ACCTGATAGA GCTCAATCTG 
AGTGGGAATA AACTAGGAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATACA 
CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGAAG CTCACTGATT TACTGCACAG TAGAAGCTGT 
AAATTCAGAT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GTGACTATCT TACTAAAGTT 
CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT 
CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GAAGAAAATC 
AAGCTGAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC 
TCCAAAACCA TCCTGAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
AGAGAAATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAAATACT CAGTCTTTCA 
GACTGCCGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGACT TACTGGAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG AAGGCTGTCA CTTTGTGACT GAAATGGTGG GTAAAAACCC ATTACTCCTG 
AAAGAACTGA ATCTGAGTGA TCGTGTACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT 
CTGCTGCAGG ATAAACACTG TCAGATCCAC ACACTCATTC TGTGTTATTG CAGTATTACA 
GAGGAACAGT GTGTGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA 
CTGAACCTCA GTGGAAACAA TCTAGGAGAC TCTGCAGTGA AAAAACTCAG TGATTTACTG 
ATGAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTG ATTGCAGTAT TACAGAGAAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTTATGGAC TTGGCCATCT GCTGAGCAGT 
GTGGATTGCA AGCTGAATAA ACTACATCTG TGTGATTGCA GTATTACAGG GAAACAGTTG 
CACTTCCTGA TTGCAGTTAT GTGTATGGAC CCATCACACC TGAGGGTACT GGACCTCAGT 
AAGAATCAAA TAAAAAGCAG AGGAATGAAC ATGCTGTGTG ATGTGCTGAA TAAACCACGC 
TGTAAACTCA AGAGTTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACACTTAACT 
CAGTCTTTGG CTGAGAACAA AGCACTGGAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT 
CAAATGGGAG ACTCCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA 
CTGAGGTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326713.1
CDS	182..5416
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471038.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471038.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GACGCCTGTC GGTTTGTGAC TAAAATTGTG GGTAAAAACC CATTACTCCT GACAGAACTG 
AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAGAGTCAA CACAATAATG CTGAATAATA ACAGGATTAC AGCAGAAGGT 
TGTGCTGCTT TAACTTCAGC ATTTATTTTA AACCCATCAC ACCTGATAGA GCTCAATCTG 
AGTGGGAATA AACTAGGAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATACA 
CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGAAG CTCACTGATT TACTGCACAG TAGAAGCTGT 
AAATTCAGAT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GTGACTATCT TACTAAAGTT 
CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT 
CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GAAGAAAATC 
AAGCTGAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC 
TCCAAAACCA TCCTGAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
AGAGAAATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAAATACT CAGTCTTTCA 
GACTGCCGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGACT TACTGGAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG AAGGCTGTCA CTTTGTGACT GAAATGGTGG GTAAAAACCC ATTACTCCTG 
AAAGAACTGA ATCTGAGTGA TCGTGTACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT 
CTGCTGCAGG ATAAACACTG TCAGATCCAC ACACTCATTC TGTGTTATTG CAGTATTACA 
GAGGAACAGT GTGTGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA 
CTGAACCTCA GTGGAAACAA TCTAGGAGAC TCTGCAGTGA AAAAACTCAG TGATTTACTG 
ATGAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTG ATTGCAGTAT TACAGAGAAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTTATGGAC TTGGCCATCT GCTGAGCAGT 
GTGGATTGCA AGCTGAATAA ACTACATCTG TGTGATTGCA GTATTACAGG GAAACAGTTG 
CACTTCCTGA TTGCAGTTAT GTGTATGGAC CCATCACACC TGAGGGTACT GGACCTCAGT 
AAGAATCAAA TAAAAAGCAG AGGAATGAAC ATGCTGTGTG ATGTGCTGAA TAAACCACGC 
TGTAAACTCA AGAGTTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACACTTAACT 
CAGTCTTTGG CTGAGAACAA AGCACTGGAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT 
CAAATGGGAG ACTCCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA 
CTGAGGTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_021471039.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326714.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X21, mRNA.

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CloneID	ODa146942
Clone ID Related Accession (Same CDS sequence)	XM_021471039.1
Accession Version	XM_021471039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTGAGCTGA CAGAGAAAAG CTGTTCACTT 
CTAGCTGCAG TTCTCTCCTC CAAAACCATC CTGAAAGAGA TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAAATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAAATACTCA GTCTTTCAGA CTGCCGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGACTTA CTGGAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCACT TTGTGACTGA AATGGTGGGT 
AAAAACCCAT TACTCCTGAA AGAACTGAAT CTGAGTGATC GTGTACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT GCTGCAGGAT AAACACTGTC AGATCCACAC ACTCATTCTG 
TGTTATTGCA GTATTACAGA GGAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAGT GGAAACAATC TAGGAGACTC TGCAGTGAAA 
AAACTCAGTG ATTTACTGAT GAACCAACAA TTCAAGCTGG AGAAACTACG TCTGAGTGAT 
TGCAGTATTA CAGAGAAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGAACCT CAATGGGAAT AGACTAGGAG ACTCTGGAGC TTATGGACTT 
GGCCATCTGC TGAGCAGTGT GGATTGCAAG CTGAATAAAC TACATCTGTG TGATTGCAGT 
ATTACAGGGA AACAGTTGCA CTTCCTGATT GCAGTTATGT GTATGGACCC ATCACACCTG 
AGGGTACTGG ACCTCAGTAA GAATCAAATA AAAAGCAGAG GAATGAACAT GCTGTGTGAT 
GTGCTGAATA AACCACGCTG TAAACTCAAG AGTTTAAGAC TAAATGACTG TGGCATTACA 
GATGTTACAC ACTTAACTCA GTCTTTGGCT GAGAACAAAG CACTGGAGTT CTTAAAAGAG 
CTTGAACTGA GTAAAAATCA AATGGGAGAC TCCGAAAAGC AAAAACTCAG TGACCTGCTG 
CGAGACTCAA ACTGTAAACT GAGGTTAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326714.1
CDS	182..5254
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVTSRRHKAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETPSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCRDVSSPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDAQKEEYFRKRLTDESLAKEIIDHVKKSKSLFIMCHIPVFCWISATVLQNILQKKRKNVKNNQADDASKTLQESNTEDTPKTLTQMYTHFLHVQIKQSRQKYDGENKPDVSWDKDAILSLGKLAFQQLLNNNLIFYETDLEACGIDVYKAEKFSGMCTEIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLILNINNRSVFDQNTRNRNVSMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNSGILKGLLTQQDHNNWSKWKILQYFFRTVDQQQDHNDQIKEEIVHYIKQKFEANLSLERSINLFHCLNELNDQTLVKEIQSHLSKGRYSSSDLSPAQWSALVFVLLTSDEEMEEFELQKFKKSDECLIRLLAVIKTCKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDSNCTLEKLRLSECGINEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDLLKPDYTLKTLRFLSPAAEEVFQFVTKIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCRLNTLMLNNNRITAEGCAALTFAFNLNPSHLIELNLSGNKLGDSGVEKICPLLENTQCQLEKLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_021471039.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471039.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTC 
TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGGC AGATTGAAGT TACTTCCAGA 
CGTCATAAAG CTCAAGAGAT ACAGGTTGAA TGCAGACATT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACCCAG TTTGATGGAG 
CTTATAACTC AGTTTTACCC AGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCGTGATGT TTCGTCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGCA 
CAAAAGGAGG AGTACTTCAG AAAAAGACTC ACAGATGAGA GTCTGGCCAA AGAAATCATT 
GATCATGTTA AAAAATCAAA GAGTCTTTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA CAGAAGAAGA GAAAAAATGT GAAAAACAAT 
CAGGCTGATG ACGCCTCCAA AACACTGCAG GAATCAAACA CTGAAGACAC TCCCAAGACT 
CTGACACAAA TGTACACACA CTTCTTGCAC GTTCAGATCA AGCAGAGCAG ACAAAAGTAT 
GATGGAGAAA ACAAACCAGA TGTGTCCTGG GATAAAGACG CCATCCTTTC ACTGGGGAAA 
CTGGCATTTC AGCAGCTGCT AAACAACAAC CTGATCTTCT ATGAAACAGA CCTGGAAGCC 
TGTGGTATTG ACGTCTATAA AGCTGAAAAG TTCTCAGGCA TGTGTACTGA GATCTTTAAG 
GAGGAAACAG GGATTGTTCT TGGTACCATG TACTGCTTTG TTCACTTGAG TGTTCAAGAG 
TTTATTGCAG CCCTTTATGC ACATCTGATT CTGAACATCA ACAATAGAAG TGTGTTTGAT 
CAAAACACAA GAAATAGAAA TGTGTCCATG ATTGATTTGC TGAAGACTGC AGTGGACAAG 
GCACTTGAGA GTGACAATGG ACACCTGGAC CTTTTCCTTC GCTTCCTCCT TGGTCTGTCA 
CTCCAGTCCA ACAGTGGAAT CTTAAAAGGC CTGTTGACAC AGCAAGACCA CAATAACTGG 
AGCAAATGGA AAATACTTCA GTACTTCTTT AGAACAGTAG ATCAGCAGCA AGACCACAAT 
GACCAGATCA AAGAGGAAAT AGTTCATTAC ATCAAGCAGA AGTTTGAAGC AAATCTTTCT 
CTTGAGAGAT CCATCAACCT GTTCCACTGT CTGAATGAAC TAAACGATCA AACTCTGGTG 
AAAGAGATTC AGTCTCACCT TAGCAAAGGA AGGTACTCAT CTTCTGACCT TTCACCTGCC 
CAGTGGTCTG CTCTGGTCTT TGTGTTGTTG ACATCAGATG AAGAGATGGA GGAGTTTGAG 
CTTCAGAAAT TCAAGAAATC AGACGAGTGT CTCATCAGGC TATTAGCTGT CATCAAAACC 
TGCAAAAGAG CACTGCTCAG TAAGTGTAAT CTAACAGAGG AAAGCTGTTC AGCTCTGGCT 
TCAGTTCTCA GATCAGACTC CAGCAGTCTG AAGGATCTTG ACCTGAGCAA TAATAATCTG 
CAGGATTCAG GAGTGAAGCT GCTCTCTGAT GGACTGAAGG ACAGTAACTG TACACTGGAG 
AAACTCAGAC TTTCAGAGTG CGGTATCAAT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACCA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG AAACCAGACT ATACACTTAA AACACTGAGG 
TTTTTGAGTC CTGCTGCAGA AGAAGTCTTT CAGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCGTTACTCC TGAAAGAACT GAATCTGAGT GATCGTGAAC TAGGAGATAC ACGAGTGAAT 
CAACTCTCTG CTCTGCTGCA GGATAAACAC TGTAGACTCA ACACACTCAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTGACTTTTG CATTTAATTT AAATCCTTCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCAA TTAGAGAAAC TGAGGCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACAGCAGG ATCCACACTG TACACTGAAG ATACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGCCTGTC AGTTTTTGAC TGGAGTTGTG GGTAAAAACC TGTTACTCTT GAAAGAGCTG 
AATCTGAGTG GACATGAACT AGGACTCTCA GACAGCTTTC AAATGCTTGT TCTTTTACTG 
CATGATAAAC ACTGTACGCT CAACACACTG ATTCTTTCAG ATTGCAGTAT CACAGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGACA TCAAACCCAT CATACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAAC TCACTGATTT ACTACTGGAT 
GAACGCTGTA GCTTAAAAAT CATCAGGTTT CTGAAAAGTC CTGCTGCACA AGAGTCATGT 
GATTTTCTCA CTGAAGTTCT GGATGTAAAT CCATTATTAC TGACAGAACT GGATCTGAGT 
GAAGATAAAT TAGGAGATCT GGATGGAGAG AAAATCTCTG CTTTTTTGAT GGACCCTCAT 
AGCAAAGTGG AGAAAATCAA GCTGAATAAT TGTGAGCTGA CAGAGAAAAG CTGTTCACTT 
CTAGCTGCAG TTCTCTCCTC CAAAACCATC CTGAAAGAGA TGAACCTGAA CAACAGTCGT 
CTGCTGGACT CAGGAGTCAG AGAAATCTGT GAGGGACTGA AGAATCCTGT GTGTGAGCTG 
AAAATACTCA GTCTTTCAGA CTGCCGTATC AGTGAAGAAG GTTATAAAGC TCTGGCTTCA 
GCTCTGAGAT CAAACCCTTC ACACCTGATA GAGCTGGATC TCAGAGGAAA TGATCCTGGA 
CAATCAGGAG TGAAGGAGCT CACTGACTTA CTGGAGGATC CACACTGTAC ACTGAAGACA 
CTGAGGTTTT TGAGTCCTGC TGCTGATGAA GGCTGTCACT TTGTGACTGA AATGGTGGGT 
AAAAACCCAT TACTCCTGAA AGAACTGAAT CTGAGTGATC GTGTACTAGG AGACACACGA 
GTGAATCAAC TCTCTGCTCT GCTGCAGGAT AAACACTGTC AGATCCACAC ACTCATTCTG 
TGTTATTGCA GTATTACAGA GGAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAGT GGAAACAATC TAGGAGACTC TGCAGTGAAA 
AAACTCAGTG ATTTACTGAT GAACCAACAA TTCAAGCTGG AGAAACTACG TCTGAGTGAT 
TGCAGTATTA CAGAGAAACA GTGTGTGATC CTGACTTCAG CTCTGAAATC AAACCCATCA 
CACCTGAGAG AACTGAACCT CAATGGGAAT AGACTAGGAG ACTCTGGAGC TTATGGACTT 
GGCCATCTGC TGAGCAGTGT GGATTGCAAG CTGAATAAAC TACATCTGTG TGATTGCAGT 
ATTACAGGGA AACAGTTGCA CTTCCTGATT GCAGTTATGT GTATGGACCC ATCACACCTG 
AGGGTACTGG ACCTCAGTAA GAATCAAATA AAAAGCAGAG GAATGAACAT GCTGTGTGAT 
GTGCTGAATA AACCACGCTG TAAACTCAAG AGTTTAAGAC TAAATGACTG TGGCATTACA 
GATGTTACAC ACTTAACTCA GTCTTTGGCT GAGAACAAAG CACTGGAGTT CTTAAAAGAG 
CTTGAACTGA GTAAAAATCA AATGGGAGAC TCCGAAAAGC AAAAACTCAG TGACCTGCTG 
CGAGACTCAA ACTGTAAACT GAGGTTAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_009298420.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296695.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X2, mRNA.

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CloneID	ODa45489
Clone ID Related Accession (Same CDS sequence)	XM_009298420.2
Accession Version	XM_009298420.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC568664 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298420.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAA ACTTTCGATT AACTTACATT TTAGAAAACA TTGAAGGATC TCAGGTGTGT 
TTGAGCTGTG ACTTAGTCTT TTTAAAGGTT TCTGGATTCT GTTTCTGCTG CACAAGACCG 
TCTTCTCGAC ATGTAAGATC AGGTTCGTAT GTATCCAGCT CTGTGTCTGT GAAGAGTGAT 
GATTCAAAAA AAGGAGGTCA GCCAAACTTC AGTAGAGAAA CACTGTCACC TACCACAAGT 
GTTCAAAATG AGTCTTCAGA CACAGACATC CAGACTCAGA AAAAACACAT GAGTTTTAAA 
GACAATCTTC AGGGGATCTT CCAGGATCTT GAGAACCAAA TATTAAAATT TCTGAAGAAT 
GAGCTAGAAA AGTTTAAGAA AATATTACAT GAAGAAAATA CAGAATACTT TGTAAAGGAC 
TTTGGTGAGA ACAGATGCAG AATAAAAGAA GCAGCTCTTG ATCTCACACT CTACTTCCTG 
AAAGAAATGA AGCAAGATGA AGCAGCAGAT ACTCTAGAAG ATGAGCTGTT TCTCATTCAT 
CAGCTGAAAT GGGATCTACA AAAGAAGTAT CAGCGTGTGT TTGAAGGAAT TGCACAGCAA 
GGAGACTCCA CACTCCTGAA GAACATCTAC ACAGATCTTT ATATCACTCA GGGTGGCAGT 
GAACAGGTCA ATACTGAACA TGAGGTCAGA CAGATTGAAG TTGTTTCTAG ACGACATGAA 
GCTCAAGAGA TACAGGTTGA ATGCAGACGT TTGTTTGAAG CGTCTGAACA AGACAAGCAG 
ATCAGAACTG TCCTGACAAA AGGAGTCGCT GGCATCGGGA AATCAGTCTC TGTGCAAAAG 
TTTGTTCTGG ATTGGGCCGA AGGAAAAGAA AATCAAGAGA TCAGCTTCAT ATTTCCTCTT 
CCATTCAGAG AGATGAACTT AAAGGAGAAA GAAACACTCA GTTTGATGGA GCTTATAACT 
CAGTTTTTCC CAGAGACGAA AGGACTGAAC CTTACAAGAA GGAATCAGTT CAAAGTCCTG 
TTCATCCTTG ATGGATTGGA TGAATGTCGT CTTCCTCTGA ACTTTGCTGG TAATGAGACG 
TGTCTAGATG TTGCATCACC AGCCTCTCTG GATGTTCTCC TGACGAACCT CATCAAGGGA 
AATCTGCTTC CTTCTGCTCT CATCTGGATC ACCAGCAGAC CAGCAGCTGC CAGTAAGATT 
CCTCCTGACT GTATCGACAG AGTGACAGAG ATACGAGGAT TCAATGATGA GCAAAAGGAG 
GAGTACTTCA GAAAAAGATT CACAGATGAG AATCAGGCCA AAGAAATCAT TGATCATGTT 
AAACAATCAA AGAGTCTCTT CATCATGTGC CACATCCCAG TCTTCTGTTG GATTTCAGCC 
ACTGTTCTCC AGAACATTTT AGAGGAGAAG AGAAATACCG TTGTGAAAAA CAATCAGGCT 
GATGACGCCT TCAAAACACT GCAGGAATCA AACACTGAAG ACACTCCCAA GACTCTGACA 
CAGATGTACA CACACTTTCT GCACTTTCAG ATCCAGCAGA GCAGACGAAA GTATGATGGA 
GAATACACAC CAGATGTGTC CTGGGATAAA GACGCCATCC TTTCACTGGG GAAACTGGCA 
TTTGATAATC TGCTAAACAG CAACCTGATC TTCTATGACA CCGACCTGGA AGCCTGTGGT 
ATTGATGTCT ATAAGGCCTC AGTTTACTCA GGCATGTGTA CCCAGATCTT TAAGGAGGAA 
ACAGGGATTG TTCTTGGCAC CATGTACTGC TTTGTTCACT TGAGCGTTCA AGAGTTTATT 
GCAGCCCTTT ATGCACATCT GTTACTAGAC ATCAACAAGA AAAGTGTGTT TGATCAAGAC 
TCTAAAAACA GAAATGAGTC CATGATTGAT TTGCTAAAGA CTGCAGTGGA CAAGACACTA 
GAGAGTGACA AAGGACACCT GGACCTTTTC CTTCGCTTCC TTCTCGGTCT GTCTCTCCAG 
TCCAATCAAC GGCTCTTACG AGGTCTGTTG ACACAGCAAG ACCACAATAA CCAGATCAAA 
GAGGAAATAG TTCAGTACAT CAAGCAGAAG TTTGAAGCGA ATCTTTCTCC AGAGAGATCC 
ATCAATCTGT TCTACTGTCT GAATGAACTG AACGACCAAA CTCTGGTGAA AGAGATTCAG 
ACTCACCTTA GCAAAGGAAG TCTCTCATCT GTTGATCTTT CACCTGCCCA GTGGTCTGCT 
CTGGCCTTTG TGTTGTTGAC ATCAGAGGAG AAGATGGAGG AGTTTGAGCT TCAGAAATTC 
AAGAAATCAG ACGAGTGTCT CATCAGACTA TCAGCAGTTA TCAAAACTAC CAGAAAAGCT 
CTGTTAAATG ATTGTAACTT AACAGACAGA AGCTGTTCAG CTCTGCCTGC AGTTCTCGGA 
GAAGAAAACA ATCTGAAAGA GCTGAACATG AGCAATAACA ATCTGCAGGA TTCAGGAGTG 
AAGCTGCTCT GCACTGGACT GAAAGATATT AAGTGCGAGT TAGAAATACT AAGACTTTCA 
GACTGCAGTA TTACAGAAGA AGGTTATAAA GCTCTGGCTT TAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGATT TACTGCAGGA TCCACACTGT ACACTGAAGA CACTAAGGTT TTTGAGTACT 
GCTGCAGAGG AAGCCTGTCA GTTTGTGCCT GGAGTTGTGG GTAAAAACCC GTTACTCCTT 
AAAGAACTGA ATCTGAGTGA TTGTAAACTA GGAGACAGGC GAGTGAACCA ACTCTCTGCT 
TTACTGCAGG ATAAACACTG TCAGATCAAC ACACTCTTTC TGTATAATTG CAGTATTACA 
GAGGAACAGT GTTTGATCCT GTCTTCAGCT CTGAAATCAA ACCCATCACA CTTGAGAAAA 
CTGAACCTCA GTGAGAATCA AATAAGAAAC ACAGGAGTGA ATCTCTTATG TGATGTACTG 
AAGGATTCAC ACTGTAAACT GAAGACACTG AGGCTTTCAG ACTGCAGTAT CAGTGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGATTT ACAGCAGGAT 
CCACACTGTA CACTGAAGAT ACTGAGGTTT TTGAGTCCTG CTGCTGATGA AGCCTGTCAG 
TTTTTGACTG GAGTTGTGGG TAAAAACCTG TTACTCTTGA AAGAGCTGAA TCTGAGTGGA 
CATGAACTAG GACTCTCAGA CAGCTTTCAA ATGCTTGTTC TTTTACTGCA TGATAAACAC 
TGTACGCTCA ACACACTGAT TCTTTCAGAT TGCAGTATCA CAGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGACATC AAACCCATCA TACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGGAACTC ACTGATTTAC TACTGGATGA ACGCTGTAGC 
TTAAAAATCA TCAGGTTTCT GAAAAGTCCT GCTGCACAAG AGTCATGTGA TTTTCTCACT 
GAAGTTCTGG ATGTAAATCC ATTATTACTG ACAGAACTGG ATCTGAGTGA AGATAAATTA 
GGAGATCTGG ATGGAGAGAA AATCTCTGCT TTTTTGATGG ACCCTCATAG CAAAGTGGAG 
AAAATCAAGC TGAATAATTG TAAGCTGCTT GAGAAAAGCT CATCAGTTTT AGCAACAGTT 
CTGTCTTCCA AAACCATCCT GAAAGAGCTG AACCTGAACA ACAGTCGTCT GCTGGACTCA 
GGAGTCAGAG AGATCTGTGA GGGACTGAAG AATCCTGTGT GTGAGCTGAA GATACTCAAG 
CTTTCAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG 
AAGGAGCTCA CTGAGTTACT GCAGGATCCA CACTGTACAC TGAAGACACT GAGGTTTTTG 
AGTCCTGCTG CTGATGAAGG CTGTCAGTTT GTGACTGGAA TTGTGGGTAA AAACCCATTA 
CTTCTGAAAG AGCTAAATCT GAGTGATCGT GAACTAGGAG ACACACGAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTGTAAA CTCAACACAC TCATTCTTAG TAAATGTGGT 
GTAACAGTGG AAAGCTGTTC AGCTCTGGCT ACAGTCCTGA GATCAGAGAC CAGTCTAAAA 
GAGCTTGACA TGAGCAACAA TAATCTGCAG GAATCAGGAG TGAAGAAACT CCAGAGCGGA 
TTAAAAAACA CAAACTGCAC ACTGGAAAAA CTCAGTCTTT TAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGA AGCTCACTGA GTTACTGAAA 
CCAGACTATA CACTGAAGAC ACTGAGGTTT TTGAGTGATG ATGCAGATGA AGCCTGTACA 
CATCTGAATA AAGTTCTGGG TGGAAACCTG TTACTCTTGA GAGAGCTGAA TCTGAGTAAA 
CTTGATCTTG GACCATCAGG ATTGGAGAAA CTTGCTGCTG TACTGAAAGA TAAACACTGT 
AAACTCAACA CACTGATTCT GAATAACAGC AGTATGTCAG AAGAAGATTG TCGTGTTTTG 
GCTGAAGCTT TTAATTCAAA CCCATCAAAT CTGACAGAGC TGAATTTGAG TGGGACTAAA 
CTCACAAACT CAGGAATGAA GAGCTTCACC ACTCTGTTTC AAAACCAACA GTGTAGACTG 
GAAAAGCTGA AGTTCAGCAG CATGTGTATT ACAGCAGAAG GTTGTGCTGC TTTGGCTTCA 
GCATTTAACT CAAATCCTTC ACACCTGATA GAGCTCAATC TGAGTGAGAA TAAACTAGGA 
GACTCAGGAG TAACAGAAAT CTCCAGTCTA CTGGCAAACT CGCAGTGCAT GCTGAAGATA 
CTCGGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTGAA GACACTGAGG 
TTTTTGAGTC CTGCTGCTGA TGACGCCTGT CGGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCATTACTCC TGACAGAACT GAATCTGAGT GATCGTAAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTAGAGTCA ACACAATAAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTAACTTCAG CATTTATTTT AAACCCATCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCGA TTAGAGAAAC TGAGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAT 
TTACTGCACA GTAGAAGCTG TAAATTCAGA TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA 
TGTGACTATC TTACTAAAGT TCTGGGTATA AGTCCATTAT TACTGACAGA ACTGGATCTG 
AGTGAAGATA AATTAGGAGA TCTGGATGGA GAGAAACTCT CTGCTCTTCT GATGGACTCT 
CATAGCAAAG TGAAGAAAAT CAAGCTGAAT AATTGTGAGC TGACAGAGAA AAGCTGTTCA 
CTTCTAGCTG CAGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT 
CGTCTGCTGG ACTCAGGAGT CAGAGAAATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG 
CTGAAAATAC TCAGTCTTTC AGACTGCCGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGGAGG ATCCACACTG TACACTGAAG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAAGGCTGTC ACTTTGTGAC TGAAATGGTG 
GGTAAAAACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGTACT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTGCTGCAG GATAAACACT GTCAGATCCA CACACTCATT 
CTGTGTTATT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGAAACA ATCTAGGAGA CTCTGCAGTG 
AAAAAACTCA GTGATTTACT GATGAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT 
GATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTTATGGA 
CTTGGCCATC TGCTGAGCAG TGTGGATTGC AAGCTGAATA AACTACATCT GTGTGATTGC 
AGTATTACAG GGAAACAGTT GCACTTCCTG ATTGCAGTTA TGTGTATGGA CCCATCACAC 
CTGAGGGTAC TGGACCTCAG TAAGAATCAA ATAAAAAGCA GAGGAATGAA CATGCTGTGT 
GATGTGCTGA ATAAACCACG CTGTAAACTC AAGAGTTTAA GACTAAATGA CTGTGGCATT 
ACAGATGTTA CACACTTAAC TCAGTCTTTG GCTGAGAACA AAGCACTGGA GTTCTTAAAA 
GAGCTTGAAC TGAGTAAAAA TCAAATGGGA GACTCCGAAA AGCAAAAACT CAGTGACCTG 
CTGCGAGACT CAAACTGTAA ACTGAGGTTA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296695.1
CDS	25..7200
Translation	MSQNFRLTYILENIEGSQVCLSCDLVFLKVSGFCFCCTRPSSRHVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_009298420.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298420.2

ORF Insert Sequence:

ATGAGCCAAA ACTTTCGATT AACTTACATT TTAGAAAACA TTGAAGGATC TCAGGTGTGT 
TTGAGCTGTG ACTTAGTCTT TTTAAAGGTT TCTGGATTCT GTTTCTGCTG CACAAGACCG 
TCTTCTCGAC ATGTAAGATC AGGTTCGTAT GTATCCAGCT CTGTGTCTGT GAAGAGTGAT 
GATTCAAAAA AAGGAGGTCA GCCAAACTTC AGTAGAGAAA CACTGTCACC TACCACAAGT 
GTTCAAAATG AGTCTTCAGA CACAGACATC CAGACTCAGA AAAAACACAT GAGTTTTAAA 
GACAATCTTC AGGGGATCTT CCAGGATCTT GAGAACCAAA TATTAAAATT TCTGAAGAAT 
GAGCTAGAAA AGTTTAAGAA AATATTACAT GAAGAAAATA CAGAATACTT TGTAAAGGAC 
TTTGGTGAGA ACAGATGCAG AATAAAAGAA GCAGCTCTTG ATCTCACACT CTACTTCCTG 
AAAGAAATGA AGCAAGATGA AGCAGCAGAT ACTCTAGAAG ATGAGCTGTT TCTCATTCAT 
CAGCTGAAAT GGGATCTACA AAAGAAGTAT CAGCGTGTGT TTGAAGGAAT TGCACAGCAA 
GGAGACTCCA CACTCCTGAA GAACATCTAC ACAGATCTTT ATATCACTCA GGGTGGCAGT 
GAACAGGTCA ATACTGAACA TGAGGTCAGA CAGATTGAAG TTGTTTCTAG ACGACATGAA 
GCTCAAGAGA TACAGGTTGA ATGCAGACGT TTGTTTGAAG CGTCTGAACA AGACAAGCAG 
ATCAGAACTG TCCTGACAAA AGGAGTCGCT GGCATCGGGA AATCAGTCTC TGTGCAAAAG 
TTTGTTCTGG ATTGGGCCGA AGGAAAAGAA AATCAAGAGA TCAGCTTCAT ATTTCCTCTT 
CCATTCAGAG AGATGAACTT AAAGGAGAAA GAAACACTCA GTTTGATGGA GCTTATAACT 
CAGTTTTTCC CAGAGACGAA AGGACTGAAC CTTACAAGAA GGAATCAGTT CAAAGTCCTG 
TTCATCCTTG ATGGATTGGA TGAATGTCGT CTTCCTCTGA ACTTTGCTGG TAATGAGACG 
TGTCTAGATG TTGCATCACC AGCCTCTCTG GATGTTCTCC TGACGAACCT CATCAAGGGA 
AATCTGCTTC CTTCTGCTCT CATCTGGATC ACCAGCAGAC CAGCAGCTGC CAGTAAGATT 
CCTCCTGACT GTATCGACAG AGTGACAGAG ATACGAGGAT TCAATGATGA GCAAAAGGAG 
GAGTACTTCA GAAAAAGATT CACAGATGAG AATCAGGCCA AAGAAATCAT TGATCATGTT 
AAACAATCAA AGAGTCTCTT CATCATGTGC CACATCCCAG TCTTCTGTTG GATTTCAGCC 
ACTGTTCTCC AGAACATTTT AGAGGAGAAG AGAAATACCG TTGTGAAAAA CAATCAGGCT 
GATGACGCCT TCAAAACACT GCAGGAATCA AACACTGAAG ACACTCCCAA GACTCTGACA 
CAGATGTACA CACACTTTCT GCACTTTCAG ATCCAGCAGA GCAGACGAAA GTATGATGGA 
GAATACACAC CAGATGTGTC CTGGGATAAA GACGCCATCC TTTCACTGGG GAAACTGGCA 
TTTGATAATC TGCTAAACAG CAACCTGATC TTCTATGACA CCGACCTGGA AGCCTGTGGT 
ATTGATGTCT ATAAGGCCTC AGTTTACTCA GGCATGTGTA CCCAGATCTT TAAGGAGGAA 
ACAGGGATTG TTCTTGGCAC CATGTACTGC TTTGTTCACT TGAGCGTTCA AGAGTTTATT 
GCAGCCCTTT ATGCACATCT GTTACTAGAC ATCAACAAGA AAAGTGTGTT TGATCAAGAC 
TCTAAAAACA GAAATGAGTC CATGATTGAT TTGCTAAAGA CTGCAGTGGA CAAGACACTA 
GAGAGTGACA AAGGACACCT GGACCTTTTC CTTCGCTTCC TTCTCGGTCT GTCTCTCCAG 
TCCAATCAAC GGCTCTTACG AGGTCTGTTG ACACAGCAAG ACCACAATAA CCAGATCAAA 
GAGGAAATAG TTCAGTACAT CAAGCAGAAG TTTGAAGCGA ATCTTTCTCC AGAGAGATCC 
ATCAATCTGT TCTACTGTCT GAATGAACTG AACGACCAAA CTCTGGTGAA AGAGATTCAG 
ACTCACCTTA GCAAAGGAAG TCTCTCATCT GTTGATCTTT CACCTGCCCA GTGGTCTGCT 
CTGGCCTTTG TGTTGTTGAC ATCAGAGGAG AAGATGGAGG AGTTTGAGCT TCAGAAATTC 
AAGAAATCAG ACGAGTGTCT CATCAGACTA TCAGCAGTTA TCAAAACTAC CAGAAAAGCT 
CTGTTAAATG ATTGTAACTT AACAGACAGA AGCTGTTCAG CTCTGCCTGC AGTTCTCGGA 
GAAGAAAACA ATCTGAAAGA GCTGAACATG AGCAATAACA ATCTGCAGGA TTCAGGAGTG 
AAGCTGCTCT GCACTGGACT GAAAGATATT AAGTGCGAGT TAGAAATACT AAGACTTTCA 
GACTGCAGTA TTACAGAAGA AGGTTATAAA GCTCTGGCTT TAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGATT TACTGCAGGA TCCACACTGT ACACTGAAGA CACTAAGGTT TTTGAGTACT 
GCTGCAGAGG AAGCCTGTCA GTTTGTGCCT GGAGTTGTGG GTAAAAACCC GTTACTCCTT 
AAAGAACTGA ATCTGAGTGA TTGTAAACTA GGAGACAGGC GAGTGAACCA ACTCTCTGCT 
TTACTGCAGG ATAAACACTG TCAGATCAAC ACACTCTTTC TGTATAATTG CAGTATTACA 
GAGGAACAGT GTTTGATCCT GTCTTCAGCT CTGAAATCAA ACCCATCACA CTTGAGAAAA 
CTGAACCTCA GTGAGAATCA AATAAGAAAC ACAGGAGTGA ATCTCTTATG TGATGTACTG 
AAGGATTCAC ACTGTAAACT GAAGACACTG AGGCTTTCAG ACTGCAGTAT CAGTGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGATTT ACAGCAGGAT 
CCACACTGTA CACTGAAGAT ACTGAGGTTT TTGAGTCCTG CTGCTGATGA AGCCTGTCAG 
TTTTTGACTG GAGTTGTGGG TAAAAACCTG TTACTCTTGA AAGAGCTGAA TCTGAGTGGA 
CATGAACTAG GACTCTCAGA CAGCTTTCAA ATGCTTGTTC TTTTACTGCA TGATAAACAC 
TGTACGCTCA ACACACTGAT TCTTTCAGAT TGCAGTATCA CAGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGACATC AAACCCATCA TACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGGAACTC ACTGATTTAC TACTGGATGA ACGCTGTAGC 
TTAAAAATCA TCAGGTTTCT GAAAAGTCCT GCTGCACAAG AGTCATGTGA TTTTCTCACT 
GAAGTTCTGG ATGTAAATCC ATTATTACTG ACAGAACTGG ATCTGAGTGA AGATAAATTA 
GGAGATCTGG ATGGAGAGAA AATCTCTGCT TTTTTGATGG ACCCTCATAG CAAAGTGGAG 
AAAATCAAGC TGAATAATTG TAAGCTGCTT GAGAAAAGCT CATCAGTTTT AGCAACAGTT 
CTGTCTTCCA AAACCATCCT GAAAGAGCTG AACCTGAACA ACAGTCGTCT GCTGGACTCA 
GGAGTCAGAG AGATCTGTGA GGGACTGAAG AATCCTGTGT GTGAGCTGAA GATACTCAAG 
CTTTCAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG 
AAGGAGCTCA CTGAGTTACT GCAGGATCCA CACTGTACAC TGAAGACACT GAGGTTTTTG 
AGTCCTGCTG CTGATGAAGG CTGTCAGTTT GTGACTGGAA TTGTGGGTAA AAACCCATTA 
CTTCTGAAAG AGCTAAATCT GAGTGATCGT GAACTAGGAG ACACACGAGT GAATCAACTC 
TCTGCTCTAC TGCAGGATAA ACACTGTAAA CTCAACACAC TCATTCTTAG TAAATGTGGT 
GTAACAGTGG AAAGCTGTTC AGCTCTGGCT ACAGTCCTGA GATCAGAGAC CAGTCTAAAA 
GAGCTTGACA TGAGCAACAA TAATCTGCAG GAATCAGGAG TGAAGAAACT CCAGAGCGGA 
TTAAAAAACA CAAACTGCAC ACTGGAAAAA CTCAGTCTTT TAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGA AGCTCACTGA GTTACTGAAA 
CCAGACTATA CACTGAAGAC ACTGAGGTTT TTGAGTGATG ATGCAGATGA AGCCTGTACA 
CATCTGAATA AAGTTCTGGG TGGAAACCTG TTACTCTTGA GAGAGCTGAA TCTGAGTAAA 
CTTGATCTTG GACCATCAGG ATTGGAGAAA CTTGCTGCTG TACTGAAAGA TAAACACTGT 
AAACTCAACA CACTGATTCT GAATAACAGC AGTATGTCAG AAGAAGATTG TCGTGTTTTG 
GCTGAAGCTT TTAATTCAAA CCCATCAAAT CTGACAGAGC TGAATTTGAG TGGGACTAAA 
CTCACAAACT CAGGAATGAA GAGCTTCACC ACTCTGTTTC AAAACCAACA GTGTAGACTG 
GAAAAGCTGA AGTTCAGCAG CATGTGTATT ACAGCAGAAG GTTGTGCTGC TTTGGCTTCA 
GCATTTAACT CAAATCCTTC ACACCTGATA GAGCTCAATC TGAGTGAGAA TAAACTAGGA 
GACTCAGGAG TAACAGAAAT CTCCAGTCTA CTGGCAAACT CGCAGTGCAT GCTGAAGATA 
CTCGGTCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACTGCAG GATCCACACT GTACACTGAA GACACTGAGG 
TTTTTGAGTC CTGCTGCTGA TGACGCCTGT CGGTTTGTGA CTAAAATTGT GGGTAAAAAC 
CCATTACTCC TGACAGAACT GAATCTGAGT GATCGTAAAC TAGGAGACAC ACGAGTGAAT 
CAACTCTCTG CTCTACTGCA GGATAAACAC TGTAGAGTCA ACACAATAAT GCTGAATAAT 
AACAGGATTA CAGCAGAAGG TTGTGCTGCT TTAACTTCAG CATTTATTTT AAACCCATCA 
CACCTGATAG AGCTCAATCT GAGTGGGAAT AAACTAGGAG ACTCAGGAGT AGAGAAGATC 
TGTCCTCTGC TGGAAAATAC ACAATGCCGA TTAGAGAAAC TGAGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAT 
TTACTGCACA GTAGAAGCTG TAAATTCAGA TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA 
TGTGACTATC TTACTAAAGT TCTGGGTATA AGTCCATTAT TACTGACAGA ACTGGATCTG 
AGTGAAGATA AATTAGGAGA TCTGGATGGA GAGAAACTCT CTGCTCTTCT GATGGACTCT 
CATAGCAAAG TGAAGAAAAT CAAGCTGAAT AATTGTGAGC TGACAGAGAA AAGCTGTTCA 
CTTCTAGCTG CAGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT 
CGTCTGCTGG ACTCAGGAGT CAGAGAAATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG 
CTGAAAATAC TCAGTCTTTC AGACTGCCGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGGAGG ATCCACACTG TACACTGAAG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAAGGCTGTC ACTTTGTGAC TGAAATGGTG 
GGTAAAAACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGTACT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTGCTGCAG GATAAACACT GTCAGATCCA CACACTCATT 
CTGTGTTATT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGAAACA ATCTAGGAGA CTCTGCAGTG 
AAAAAACTCA GTGATTTACT GATGAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT 
GATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTTATGGA 
CTTGGCCATC TGCTGAGCAG TGTGGATTGC AAGCTGAATA AACTACATCT GTGTGATTGC 
AGTATTACAG GGAAACAGTT GCACTTCCTG ATTGCAGTTA TGTGTATGGA CCCATCACAC 
CTGAGGGTAC TGGACCTCAG TAAGAATCAA ATAAAAAGCA GAGGAATGAA CATGCTGTGT 
GATGTGCTGA ATAAACCACG CTGTAAACTC AAGAGTTTAA GACTAAATGA CTGTGGCATT 
ACAGATGTTA CACACTTAAC TCAGTCTTTG GCTGAGAACA AAGCACTGGA GTTCTTAAAA 
GAGCTTGAAC TGAGTAAAAA TCAAATGGGA GACTCCGAAA AGCAAAAACT CAGTGACCTG 
CTGCGAGACT CAAACTGTAA ACTGAGGTTA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_009298421.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296696.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X3, mRNA.

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CloneID	ODa45490
Clone ID Related Accession (Same CDS sequence)	XM_009298421.2
Accession Version	XM_009298421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC568664 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGG AGCGAGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAG ATCAGGTTCG 
TATGTATCCA GCTCTGTGTC TGTGAAGAGT GATGATTCAA AAAAAGGAGG TCAGCCAAAC 
TTCAGTAGAG AAACACTGTC ACCTACCACA AGTGTTCAAA ATGAGTCTTC AGACACAGAC 
ATCCAGACTC AGAAAAAACA CATGAGTTTT AAAGACAATC TTCAGGGGAT CTTCCAGGAT 
CTTGAGAACC AAATATTAAA ATTTCTGAAG AATGAGCTAG AAAAGTTTAA GAAAATATTA 
CATGAAGAAA ATACAGAATA CTTTGTAAAG GACTTTGGTG AGAACAGATG CAGAATAAAA 
GAAGCAGCTC TTGATCTCAC ACTCTACTTC CTGAAAGAAA TGAAGCAAGA TGAAGCAGCA 
GATACTCTAG AAGATGAGCT GTTTCTCATT CATCAGCTGA AATGGGATCT ACAAAAGAAG 
TATCAGCGTG TGTTTGAAGG AATTGCACAG CAAGGAGACT CCACACTCCT GAAGAACATC 
TACACAGATC TTTATATCAC TCAGGGTGGC AGTGAACAGG TCAATACTGA ACATGAGGTC 
AGACAGATTG AAGTTGTTTC TAGACGACAT GAAGCTCAAG AGATACAGGT TGAATGCAGA 
CGTTTGTTTG AAGCGTCTGA ACAAGACAAG CAGATCAGAA CTGTCCTGAC AAAAGGAGTC 
GCTGGCATCG GGAAATCAGT CTCTGTGCAA AAGTTTGTTC TGGATTGGGC CGAAGGAAAA 
GAAAATCAAG AGATCAGCTT CATATTTCCT CTTCCATTCA GAGAGATGAA CTTAAAGGAG 
AAAGAAACAC TCAGTTTGAT GGAGCTTATA ACTCAGTTTT TCCCAGAGAC GAAAGGACTG 
AACCTTACAA GAAGGAATCA GTTCAAAGTC CTGTTCATCC TTGATGGATT GGATGAATGT 
CGTCTTCCTC TGAACTTTGC TGGTAATGAG ACGTGTCTAG ATGTTGCATC ACCAGCCTCT 
CTGGATGTTC TCCTGACGAA CCTCATCAAG GGAAATCTGC TTCCTTCTGC TCTCATCTGG 
ATCACCAGCA GACCAGCAGC TGCCAGTAAG ATTCCTCCTG ACTGTATCGA CAGAGTGACA 
GAGATACGAG GATTCAATGA TGAGCAAAAG GAGGAGTACT TCAGAAAAAG ATTCACAGAT 
GAGAATCAGG CCAAAGAAAT CATTGATCAT GTTAAACAAT CAAAGAGTCT CTTCATCATG 
TGCCACATCC CAGTCTTCTG TTGGATTTCA GCCACTGTTC TCCAGAACAT TTTAGAGGAG 
AAGAGAAATA CCGTTGTGAA AAACAATCAG GCTGATGACG CCTTCAAAAC ACTGCAGGAA 
TCAAACACTG AAGACACTCC CAAGACTCTG ACACAGATGT ACACACACTT TCTGCACTTT 
CAGATCCAGC AGAGCAGACG AAAGTATGAT GGAGAATACA CACCAGATGT GTCCTGGGAT 
AAAGACGCCA TCCTTTCACT GGGGAAACTG GCATTTGATA ATCTGCTAAA CAGCAACCTG 
ATCTTCTATG ACACCGACCT GGAAGCCTGT GGTATTGATG TCTATAAGGC CTCAGTTTAC 
TCAGGCATGT GTACCCAGAT CTTTAAGGAG GAAACAGGGA TTGTTCTTGG CACCATGTAC 
TGCTTTGTTC ACTTGAGCGT TCAAGAGTTT ATTGCAGCCC TTTATGCACA TCTGTTACTA 
GACATCAACA AGAAAAGTGT GTTTGATCAA GACTCTAAAA ACAGAAATGA GTCCATGATT 
GATTTGCTAA AGACTGCAGT GGACAAGACA CTAGAGAGTG ACAAAGGACA CCTGGACCTT 
TTCCTTCGCT TCCTTCTCGG TCTGTCTCTC CAGTCCAATC AACGGCTCTT ACGAGGTCTG 
TTGACACAGC AAGACCACAA TAACCAGATC AAAGAGGAAA TAGTTCAGTA CATCAAGCAG 
AAGTTTGAAG CGAATCTTTC TCCAGAGAGA TCCATCAATC TGTTCTACTG TCTGAATGAA 
CTGAACGACC AAACTCTGGT GAAAGAGATT CAGACTCACC TTAGCAAAGG AAGTCTCTCA 
TCTGTTGATC TTTCACCTGC CCAGTGGTCT GCTCTGGCCT TTGTGTTGTT GACATCAGAG 
GAGAAGATGG AGGAGTTTGA GCTTCAGAAA TTCAAGAAAT CAGACGAGTG TCTCATCAGA 
CTATCAGCAG TTATCAAAAC TACCAGAAAA GCTCTGTTAA ATGATTGTAA CTTAACAGAC 
AGAAGCTGTT CAGCTCTGCC TGCAGTTCTC GGAGAAGAAA ACAATCTGAA AGAGCTGAAC 
ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGG ACTGAAAGAT 
ATTAAGTGCG AGTTAGAAAT ACTAAGACTT TCAGACTGCA GTATTACAGA AGAAGGTTAT 
AAAGCTCTGG CTTTAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ATTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTAAG GTTTTTGAGT ACTGCTGCAG AGGAAGCCTG TCAGTTTGTG 
CCTGGAGTTG TGGGTAAAAA CCCGTTACTC CTTAAAGAAC TGAATCTGAG TGATTGTAAA 
CTAGGAGACA GGCGAGTGAA CCAACTCTCT GCTTTACTGC AGGATAAACA CTGTCAGATC 
AACACACTCT TTCTGTATAA TTGCAGTATT ACAGAGGAAC AGTGTTTGAT CCTGTCTTCA 
GCTCTGAAAT CAAACCCATC ACACTTGAGA AAACTGAACC TCAGTGAGAA TCAAATAAGA 
AACACAGGAG TGAATCTCTT ATGTGATGTA CTGAAGGATT CACACTGTAA ACTGAAGACA 
CTGAGGCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACAGCAG GATCCACACT GTACACTGAA GATACTGAGG 
TTTTTGAGTC CTGCTGCTGA TGAAGCCTGT CAGTTTTTGA CTGGAGTTGT GGGTAAAAAC 
CTGTTACTCT TGAAAGAGCT GAATCTGAGT GGACATGAAC TAGGACTCTC AGACAGCTTT 
CAAATGCTTG TTCTTTTACT GCATGATAAA CACTGTACGC TCAACACACT GATTCTTTCA 
GATTGCAGTA TCACAGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAC ATCAAACCCA 
TCATACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAA 
CTCACTGATT TACTACTGGA TGAACGCTGT AGCTTAAAAA TCATCAGGTT TCTGAAAAGT 
CCTGCTGCAC AAGAGTCATG TGATTTTCTC ACTGAAGTTC TGGATGTAAA TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGATAAA TTAGGAGATC TGGATGGAGA GAAAATCTCT 
GCTTTTTTGA TGGACCCTCA TAGCAAAGTG GAGAAAATCA AGCTGAATAA TTGTAAGCTG 
CTTGAGAAAA GCTCATCAGT TTTAGCAACA GTTCTGTCTT CCAAAACCAT CCTGAAAGAG 
CTGAACCTGA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA GAGAGATCTG TGAGGGACTG 
AAGAATCCTG TGTGTGAGCT GAAGATACTC AAGCTTTCAG ACTGCAGTAT CAGTGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGAGTT ACTGCAGGAT 
CCACACTGTA CACTGAAGAC ACTGAGGTTT TTGAGTCCTG CTGCTGATGA AGGCTGTCAG 
TTTGTGACTG GAATTGTGGG TAAAAACCCA TTACTTCTGA AAGAGCTAAA TCTGAGTGAT 
CGTGAACTAG GAGACACACG AGTGAATCAA CTCTCTGCTC TACTGCAGGA TAAACACTGT 
AAACTCAACA CACTCATTCT TAGTAAATGT GGTGTAACAG TGGAAAGCTG TTCAGCTCTG 
GCTACAGTCC TGAGATCAGA GACCAGTCTA AAAGAGCTTG ACATGAGCAA CAATAATCTG 
CAGGAATCAG GAGTGAAGAA ACTCCAGAGC GGATTAAAAA ACACAAACTG CACACTGGAA 
AAACTCAGTC TTTTAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGAAGCTCAC TGAGTTACTG AAACCAGACT ATACACTGAA GACACTGAGG 
TTTTTGAGTG ATGATGCAGA TGAAGCCTGT ACACATCTGA ATAAAGTTCT GGGTGGAAAC 
CTGTTACTCT TGAGAGAGCT GAATCTGAGT AAACTTGATC TTGGACCATC AGGATTGGAG 
AAACTTGCTG CTGTACTGAA AGATAAACAC TGTAAACTCA ACACACTGAT TCTGAATAAC 
AGCAGTATGT CAGAAGAAGA TTGTCGTGTT TTGGCTGAAG CTTTTAATTC AAACCCATCA 
AATCTGACAG AGCTGAATTT GAGTGGGACT AAACTCACAA ACTCAGGAAT GAAGAGCTTC 
ACCACTCTGT TTCAAAACCA ACAGTGTAGA CTGGAAAAGC TGAAGTTCAG CAGCATGTGT 
ATTACAGCAG AAGGTTGTGC TGCTTTGGCT TCAGCATTTA ACTCAAATCC TTCACACCTG 
ATAGAGCTCA ATCTGAGTGA GAATAAACTA GGAGACTCAG GAGTAACAGA AATCTCCAGT 
CTACTGGCAA ACTCGCAGTG CATGCTGAAG ATACTCGGTC TTTCAGACTG CAGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG 
CAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGACGCC 
TGTCGGTTTG TGACTAAAAT TGTGGGTAAA AACCCATTAC TCCTGACAGA ACTGAATCTG 
AGTGATCGTA AACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGTAGAG TCAACACAAT AATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT 
GCTTTAACTT CAGCATTTAT TTTAAACCCA TCACACCTGA TAGAGCTCAA TCTGAGTGGG 
AATAAACTAG GAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TACACAATGC 
CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GAAGCTCACT GATTTACTGC ACAGTAGAAG CTGTAAATTC 
AGATTTCTGA AGAGTTCTGC TGCACAGGAA GCATGTGACT ATCTTACTAA AGTTCTGGGT 
ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT 
GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGAAGAA AATCAAGCTG 
AATAATTGTG AGCTGACAGA GAAAAGCTGT TCACTTCTAG CTGCAGTTCT CTCCTCCAAA 
ACCATCCTGA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAGAA 
ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAAA TACTCAGTCT TTCAGACTGC 
CGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GACTTACTGG AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT 
GATGAAGGCT GTCACTTTGT GACTGAAATG GTGGGTAAAA ACCCATTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTGT ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTGCTG 
CAGGATAAAC ACTGTCAGAT CCACACACTC ATTCTGTGTT ATTGCAGTAT TACAGAGGAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAGTGGAA ACAATCTAGG AGACTCTGCA GTGAAAAAAC TCAGTGATTT ACTGATGAAC 
CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTGATTGCA GTATTACAGA GAAACAGTGT 
GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT 
GGGAATAGAC TAGGAGACTC TGGAGCTTAT GGACTTGGCC ATCTGCTGAG CAGTGTGGAT 
TGCAAGCTGA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGGGAAACA GTTGCACTTC 
CTGATTGCAG TTATGTGTAT GGACCCATCA CACCTGAGGG TACTGGACCT CAGTAAGAAT 
CAAATAAAAA GCAGAGGAAT GAACATGCTG TGTGATGTGC TGAATAAACC ACGCTGTAAA 
CTCAAGAGTT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACACTT AACTCAGTCT 
TTGGCTGAGA ACAAAGCACT GGAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG 
GGAGACTCCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG 
TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296696.1
CDS	117..7205
Translation	MSEERGKMSLSEKHSVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_009298421.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298421.2

ORF Insert Sequence:

ATGTCAGAGG AGCGAGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAG ATCAGGTTCG 
TATGTATCCA GCTCTGTGTC TGTGAAGAGT GATGATTCAA AAAAAGGAGG TCAGCCAAAC 
TTCAGTAGAG AAACACTGTC ACCTACCACA AGTGTTCAAA ATGAGTCTTC AGACACAGAC 
ATCCAGACTC AGAAAAAACA CATGAGTTTT AAAGACAATC TTCAGGGGAT CTTCCAGGAT 
CTTGAGAACC AAATATTAAA ATTTCTGAAG AATGAGCTAG AAAAGTTTAA GAAAATATTA 
CATGAAGAAA ATACAGAATA CTTTGTAAAG GACTTTGGTG AGAACAGATG CAGAATAAAA 
GAAGCAGCTC TTGATCTCAC ACTCTACTTC CTGAAAGAAA TGAAGCAAGA TGAAGCAGCA 
GATACTCTAG AAGATGAGCT GTTTCTCATT CATCAGCTGA AATGGGATCT ACAAAAGAAG 
TATCAGCGTG TGTTTGAAGG AATTGCACAG CAAGGAGACT CCACACTCCT GAAGAACATC 
TACACAGATC TTTATATCAC TCAGGGTGGC AGTGAACAGG TCAATACTGA ACATGAGGTC 
AGACAGATTG AAGTTGTTTC TAGACGACAT GAAGCTCAAG AGATACAGGT TGAATGCAGA 
CGTTTGTTTG AAGCGTCTGA ACAAGACAAG CAGATCAGAA CTGTCCTGAC AAAAGGAGTC 
GCTGGCATCG GGAAATCAGT CTCTGTGCAA AAGTTTGTTC TGGATTGGGC CGAAGGAAAA 
GAAAATCAAG AGATCAGCTT CATATTTCCT CTTCCATTCA GAGAGATGAA CTTAAAGGAG 
AAAGAAACAC TCAGTTTGAT GGAGCTTATA ACTCAGTTTT TCCCAGAGAC GAAAGGACTG 
AACCTTACAA GAAGGAATCA GTTCAAAGTC CTGTTCATCC TTGATGGATT GGATGAATGT 
CGTCTTCCTC TGAACTTTGC TGGTAATGAG ACGTGTCTAG ATGTTGCATC ACCAGCCTCT 
CTGGATGTTC TCCTGACGAA CCTCATCAAG GGAAATCTGC TTCCTTCTGC TCTCATCTGG 
ATCACCAGCA GACCAGCAGC TGCCAGTAAG ATTCCTCCTG ACTGTATCGA CAGAGTGACA 
GAGATACGAG GATTCAATGA TGAGCAAAAG GAGGAGTACT TCAGAAAAAG ATTCACAGAT 
GAGAATCAGG CCAAAGAAAT CATTGATCAT GTTAAACAAT CAAAGAGTCT CTTCATCATG 
TGCCACATCC CAGTCTTCTG TTGGATTTCA GCCACTGTTC TCCAGAACAT TTTAGAGGAG 
AAGAGAAATA CCGTTGTGAA AAACAATCAG GCTGATGACG CCTTCAAAAC ACTGCAGGAA 
TCAAACACTG AAGACACTCC CAAGACTCTG ACACAGATGT ACACACACTT TCTGCACTTT 
CAGATCCAGC AGAGCAGACG AAAGTATGAT GGAGAATACA CACCAGATGT GTCCTGGGAT 
AAAGACGCCA TCCTTTCACT GGGGAAACTG GCATTTGATA ATCTGCTAAA CAGCAACCTG 
ATCTTCTATG ACACCGACCT GGAAGCCTGT GGTATTGATG TCTATAAGGC CTCAGTTTAC 
TCAGGCATGT GTACCCAGAT CTTTAAGGAG GAAACAGGGA TTGTTCTTGG CACCATGTAC 
TGCTTTGTTC ACTTGAGCGT TCAAGAGTTT ATTGCAGCCC TTTATGCACA TCTGTTACTA 
GACATCAACA AGAAAAGTGT GTTTGATCAA GACTCTAAAA ACAGAAATGA GTCCATGATT 
GATTTGCTAA AGACTGCAGT GGACAAGACA CTAGAGAGTG ACAAAGGACA CCTGGACCTT 
TTCCTTCGCT TCCTTCTCGG TCTGTCTCTC CAGTCCAATC AACGGCTCTT ACGAGGTCTG 
TTGACACAGC AAGACCACAA TAACCAGATC AAAGAGGAAA TAGTTCAGTA CATCAAGCAG 
AAGTTTGAAG CGAATCTTTC TCCAGAGAGA TCCATCAATC TGTTCTACTG TCTGAATGAA 
CTGAACGACC AAACTCTGGT GAAAGAGATT CAGACTCACC TTAGCAAAGG AAGTCTCTCA 
TCTGTTGATC TTTCACCTGC CCAGTGGTCT GCTCTGGCCT TTGTGTTGTT GACATCAGAG 
GAGAAGATGG AGGAGTTTGA GCTTCAGAAA TTCAAGAAAT CAGACGAGTG TCTCATCAGA 
CTATCAGCAG TTATCAAAAC TACCAGAAAA GCTCTGTTAA ATGATTGTAA CTTAACAGAC 
AGAAGCTGTT CAGCTCTGCC TGCAGTTCTC GGAGAAGAAA ACAATCTGAA AGAGCTGAAC 
ATGAGCAATA ACAATCTGCA GGATTCAGGA GTGAAGCTGC TCTGCACTGG ACTGAAAGAT 
ATTAAGTGCG AGTTAGAAAT ACTAAGACTT TCAGACTGCA GTATTACAGA AGAAGGTTAT 
AAAGCTCTGG CTTTAGCTCT GAGATCAAAC CCTTCACACC TGATAGAGCT GGATCTCAGA 
GGAAATGATC CTGGACAATC AGGAGTGAAG GAGCTCACTG ATTTACTGCA GGATCCACAC 
TGTACACTGA AGACACTAAG GTTTTTGAGT ACTGCTGCAG AGGAAGCCTG TCAGTTTGTG 
CCTGGAGTTG TGGGTAAAAA CCCGTTACTC CTTAAAGAAC TGAATCTGAG TGATTGTAAA 
CTAGGAGACA GGCGAGTGAA CCAACTCTCT GCTTTACTGC AGGATAAACA CTGTCAGATC 
AACACACTCT TTCTGTATAA TTGCAGTATT ACAGAGGAAC AGTGTTTGAT CCTGTCTTCA 
GCTCTGAAAT CAAACCCATC ACACTTGAGA AAACTGAACC TCAGTGAGAA TCAAATAAGA 
AACACAGGAG TGAATCTCTT ATGTGATGTA CTGAAGGATT CACACTGTAA ACTGAAGACA 
CTGAGGCTTT CAGACTGCAG TATCAGTGAA GAAGGTTATA AAGCTCTGGC TTCAGCTCTG 
AGATCAAACC CTTCACACCT GATAGAGCTG GATCTCAGAG GAAATGATCC TGGACAATCA 
GGAGTGAAGG AGCTCACTGA TTTACAGCAG GATCCACACT GTACACTGAA GATACTGAGG 
TTTTTGAGTC CTGCTGCTGA TGAAGCCTGT CAGTTTTTGA CTGGAGTTGT GGGTAAAAAC 
CTGTTACTCT TGAAAGAGCT GAATCTGAGT GGACATGAAC TAGGACTCTC AGACAGCTTT 
CAAATGCTTG TTCTTTTACT GCATGATAAA CACTGTACGC TCAACACACT GATTCTTTCA 
GATTGCAGTA TCACAGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAC ATCAAACCCA 
TCATACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAA 
CTCACTGATT TACTACTGGA TGAACGCTGT AGCTTAAAAA TCATCAGGTT TCTGAAAAGT 
CCTGCTGCAC AAGAGTCATG TGATTTTCTC ACTGAAGTTC TGGATGTAAA TCCATTATTA 
CTGACAGAAC TGGATCTGAG TGAAGATAAA TTAGGAGATC TGGATGGAGA GAAAATCTCT 
GCTTTTTTGA TGGACCCTCA TAGCAAAGTG GAGAAAATCA AGCTGAATAA TTGTAAGCTG 
CTTGAGAAAA GCTCATCAGT TTTAGCAACA GTTCTGTCTT CCAAAACCAT CCTGAAAGAG 
CTGAACCTGA ACAACAGTCG TCTGCTGGAC TCAGGAGTCA GAGAGATCTG TGAGGGACTG 
AAGAATCCTG TGTGTGAGCT GAAGATACTC AAGCTTTCAG ACTGCAGTAT CAGTGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGGAGC TCACTGAGTT ACTGCAGGAT 
CCACACTGTA CACTGAAGAC ACTGAGGTTT TTGAGTCCTG CTGCTGATGA AGGCTGTCAG 
TTTGTGACTG GAATTGTGGG TAAAAACCCA TTACTTCTGA AAGAGCTAAA TCTGAGTGAT 
CGTGAACTAG GAGACACACG AGTGAATCAA CTCTCTGCTC TACTGCAGGA TAAACACTGT 
AAACTCAACA CACTCATTCT TAGTAAATGT GGTGTAACAG TGGAAAGCTG TTCAGCTCTG 
GCTACAGTCC TGAGATCAGA GACCAGTCTA AAAGAGCTTG ACATGAGCAA CAATAATCTG 
CAGGAATCAG GAGTGAAGAA ACTCCAGAGC GGATTAAAAA ACACAAACTG CACACTGGAA 
AAACTCAGTC TTTTAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGAAGCTCAC TGAGTTACTG AAACCAGACT ATACACTGAA GACACTGAGG 
TTTTTGAGTG ATGATGCAGA TGAAGCCTGT ACACATCTGA ATAAAGTTCT GGGTGGAAAC 
CTGTTACTCT TGAGAGAGCT GAATCTGAGT AAACTTGATC TTGGACCATC AGGATTGGAG 
AAACTTGCTG CTGTACTGAA AGATAAACAC TGTAAACTCA ACACACTGAT TCTGAATAAC 
AGCAGTATGT CAGAAGAAGA TTGTCGTGTT TTGGCTGAAG CTTTTAATTC AAACCCATCA 
AATCTGACAG AGCTGAATTT GAGTGGGACT AAACTCACAA ACTCAGGAAT GAAGAGCTTC 
ACCACTCTGT TTCAAAACCA ACAGTGTAGA CTGGAAAAGC TGAAGTTCAG CAGCATGTGT 
ATTACAGCAG AAGGTTGTGC TGCTTTGGCT TCAGCATTTA ACTCAAATCC TTCACACCTG 
ATAGAGCTCA ATCTGAGTGA GAATAAACTA GGAGACTCAG GAGTAACAGA AATCTCCAGT 
CTACTGGCAA ACTCGCAGTG CATGCTGAAG ATACTCGGTC TTTCAGACTG CAGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGATTTACTG 
CAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGACGCC 
TGTCGGTTTG TGACTAAAAT TGTGGGTAAA AACCCATTAC TCCTGACAGA ACTGAATCTG 
AGTGATCGTA AACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTACT GCAGGATAAA 
CACTGTAGAG TCAACACAAT AATGCTGAAT AATAACAGGA TTACAGCAGA AGGTTGTGCT 
GCTTTAACTT CAGCATTTAT TTTAAACCCA TCACACCTGA TAGAGCTCAA TCTGAGTGGG 
AATAAACTAG GAGACTCAGG AGTAGAGAAG ATCTGTCCTC TGCTGGAAAA TACACAATGC 
CGATTAGAGA AACTGAGTCT TTCAGACTGC AGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GAAGCTCACT GATTTACTGC ACAGTAGAAG CTGTAAATTC 
AGATTTCTGA AGAGTTCTGC TGCACAGGAA GCATGTGACT ATCTTACTAA AGTTCTGGGT 
ATAAGTCCAT TATTACTGAC AGAACTGGAT CTGAGTGAAG ATAAATTAGG AGATCTGGAT 
GGAGAGAAAC TCTCTGCTCT TCTGATGGAC TCTCATAGCA AAGTGAAGAA AATCAAGCTG 
AATAATTGTG AGCTGACAGA GAAAAGCTGT TCACTTCTAG CTGCAGTTCT CTCCTCCAAA 
ACCATCCTGA AAGAGATGAA CCTGAACAAC AGTCGTCTGC TGGACTCAGG AGTCAGAGAA 
ATCTGTGAGG GACTGAAGAA TCCTGTGTGT GAGCTGAAAA TACTCAGTCT TTCAGACTGC 
CGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GACTTACTGG AGGATCCACA CTGTACACTG AAGACACTGA GGTTTTTGAG TCCTGCTGCT 
GATGAAGGCT GTCACTTTGT GACTGAAATG GTGGGTAAAA ACCCATTACT CCTGAAAGAA 
CTGAATCTGA GTGATCGTGT ACTAGGAGAC ACACGAGTGA ATCAACTCTC TGCTCTGCTG 
CAGGATAAAC ACTGTCAGAT CCACACACTC ATTCTGTGTT ATTGCAGTAT TACAGAGGAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAGTGGAA ACAATCTAGG AGACTCTGCA GTGAAAAAAC TCAGTGATTT ACTGATGAAC 
CAACAATTCA AGCTGGAGAA ACTACGTCTG AGTGATTGCA GTATTACAGA GAAACAGTGT 
GTGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GAACCTCAAT 
GGGAATAGAC TAGGAGACTC TGGAGCTTAT GGACTTGGCC ATCTGCTGAG CAGTGTGGAT 
TGCAAGCTGA ATAAACTACA TCTGTGTGAT TGCAGTATTA CAGGGAAACA GTTGCACTTC 
CTGATTGCAG TTATGTGTAT GGACCCATCA CACCTGAGGG TACTGGACCT CAGTAAGAAT 
CAAATAAAAA GCAGAGGAAT GAACATGCTG TGTGATGTGC TGAATAAACC ACGCTGTAAA 
CTCAAGAGTT TAAGACTAAA TGACTGTGGC ATTACAGATG TTACACACTT AACTCAGTCT 
TTGGCTGAGA ACAAAGCACT GGAGTTCTTA AAAGAGCTTG AACTGAGTAA AAATCAAATG 
GGAGACTCCG AAAAGCAAAA ACTCAGTGAC CTGCTGCGAG ACTCAAACTG TAAACTGAGG 
TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_009298422.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296697.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X4, mRNA.

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CloneID	ODa45491
Clone ID Related Accession (Same CDS sequence)	XM_009298422.2
Accession Version	XM_009298422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC568664 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG 
TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA 
GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT 
CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT 
CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG 
TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT 
TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT 
AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG 
CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG 
ACACTGAGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGAAGCTCAC TGATTTACTG CACAGTAGAA GCTGTAAATT CAGATTTCTG 
AAGAGTTCTG CTGCACAGGA AGCATGTGAC TATCTTACTA AAGTTCTGGG TATAAGTCCA 
TTATTACTGA CAGAACTGGA TCTGAGTGAA GATAAATTAG GAGATCTGGA TGGAGAGAAA 
CTCTCTGCTC TTCTGATGGA CTCTCATAGC AAAGTGAAGA AAATCAAGCT GAATAATTGT 
GAGCTGACAG AGAAAAGCTG TTCACTTCTA GCTGCAGTTC TCTCCTCCAA AACCATCCTG 
AAAGAGATGA ACCTGAACAA CAGTCGTCTG CTGGACTCAG GAGTCAGAGA AATCTGTGAG 
GGACTGAAGA ATCCTGTGTG TGAGCTGAAA ATACTCAGTC TTTCAGACTG CCGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGACTTACTG 
GAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGAAGGC 
TGTCACTTTG TGACTGAAAT GGTGGGTAAA AACCCATTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCGTG TACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTGCT GCAGGATAAA 
CACTGTCAGA TCCACACACT CATTCTGTGT TATTGCAGTA TTACAGAGGA ACAGTGTGTG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGGA 
AACAATCTAG GAGACTCTGC AGTGAAAAAA CTCAGTGATT TACTGATGAA CCAACAATTC 
AAGCTGGAGA AACTACGTCT GAGTGATTGC AGTATTACAG AGAAACAGTG TGTGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAA TGGGAATAGA 
CTAGGAGACT CTGGAGCTTA TGGACTTGGC CATCTGCTGA GCAGTGTGGA TTGCAAGCTG 
AATAAACTAC ATCTGTGTGA TTGCAGTATT ACAGGGAAAC AGTTGCACTT CCTGATTGCA 
GTTATGTGTA TGGACCCATC ACACCTGAGG GTACTGGACC TCAGTAAGAA TCAAATAAAA 
AGCAGAGGAA TGAACATGCT GTGTGATGTG CTGAATAAAC CACGCTGTAA ACTCAAGAGT 
TTAAGACTAA ATGACTGTGG CATTACAGAT GTTACACACT TAACTCAGTC TTTGGCTGAG 
AACAAAGCAC TGGAGTTCTT AAAAGAGCTT GAACTGAGTA AAAATCAAAT GGGAGACTCC 
GAAAAGCAAA AACTCAGTGA CCTGCTGCGA GACTCAAACT GTAAACTGAG GTTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296697.1
CDS	182..7081
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_009298422.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298422.2

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG 
TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA 
GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT 
CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT 
CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG 
TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT 
TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT 
AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG 
CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG 
ACACTGAGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGAAGCTCAC TGATTTACTG CACAGTAGAA GCTGTAAATT CAGATTTCTG 
AAGAGTTCTG CTGCACAGGA AGCATGTGAC TATCTTACTA AAGTTCTGGG TATAAGTCCA 
TTATTACTGA CAGAACTGGA TCTGAGTGAA GATAAATTAG GAGATCTGGA TGGAGAGAAA 
CTCTCTGCTC TTCTGATGGA CTCTCATAGC AAAGTGAAGA AAATCAAGCT GAATAATTGT 
GAGCTGACAG AGAAAAGCTG TTCACTTCTA GCTGCAGTTC TCTCCTCCAA AACCATCCTG 
AAAGAGATGA ACCTGAACAA CAGTCGTCTG CTGGACTCAG GAGTCAGAGA AATCTGTGAG 
GGACTGAAGA ATCCTGTGTG TGAGCTGAAA ATACTCAGTC TTTCAGACTG CCGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGACTTACTG 
GAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGAAGGC 
TGTCACTTTG TGACTGAAAT GGTGGGTAAA AACCCATTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCGTG TACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTGCT GCAGGATAAA 
CACTGTCAGA TCCACACACT CATTCTGTGT TATTGCAGTA TTACAGAGGA ACAGTGTGTG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGGA 
AACAATCTAG GAGACTCTGC AGTGAAAAAA CTCAGTGATT TACTGATGAA CCAACAATTC 
AAGCTGGAGA AACTACGTCT GAGTGATTGC AGTATTACAG AGAAACAGTG TGTGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAA TGGGAATAGA 
CTAGGAGACT CTGGAGCTTA TGGACTTGGC CATCTGCTGA GCAGTGTGGA TTGCAAGCTG 
AATAAACTAC ATCTGTGTGA TTGCAGTATT ACAGGGAAAC AGTTGCACTT CCTGATTGCA 
GTTATGTGTA TGGACCCATC ACACCTGAGG GTACTGGACC TCAGTAAGAA TCAAATAAAA 
AGCAGAGGAA TGAACATGCT GTGTGATGTG CTGAATAAAC CACGCTGTAA ACTCAAGAGT 
TTAAGACTAA ATGACTGTGG CATTACAGAT GTTACACACT TAACTCAGTC TTTGGCTGAG 
AACAAAGCAC TGGAGTTCTT AAAAGAGCTT GAACTGAGTA AAAATCAAAT GGGAGACTCC 
GAAAAGCAAA AACTCAGTGA CCTGCTGCGA GACTCAAACT GTAAACTGAG GTTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_009298423.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296698.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X5, mRNA.

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CloneID	ODa45492
Clone ID Related Accession (Same CDS sequence)	XM_009298423.2
Accession Version	XM_009298423.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC568664 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA 
AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG 
GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT 
AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA 
TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC 
TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG 
TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC 
AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC 
GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT 
CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT 
GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA 
TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA 
TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG 
GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG 
CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC 
AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA 
GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC 
TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT 
GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA 
GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG 
CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG 
GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG 
AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG 
GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC 
TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG 
AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT 
AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG 
TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA 
ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG 
AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296698.1
CDS	182..6913
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_009298423.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298423.2

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTGATGATGC AGATGAAGCC TGTACACATC TGAATAAAGT TCTGGGTGGA 
AACCTGTTAC TCTTGAGAGA GCTGAATCTG AGTAAACTTG ATCTTGGACC ATCAGGATTG 
GAGAAACTTG CTGCTGTACT GAAAGATAAA CACTGTAAAC TCAACACACT GATTCTGAAT 
AACAGCAGTA TGTCAGAAGA AGATTGTCGT GTTTTGGCTG AAGCTTTTAA TTCAAACCCA 
TCAAATCTGA CAGAGCTGAA TTTGAGTGGG ACTAAACTCA CAAACTCAGG AATGAAGAGC 
TTCACCACTC TGTTTCAAAA CCAACAGTGT AGACTGGAAA AGCTGAAGTT CAGCAGCATG 
TGTATTACAG CAGAAGGTTG TGCTGCTTTG GCTTCAGCAT TTAACTCAAA TCCTTCACAC 
CTGATAGAGC TCAATCTGAG TGAGAATAAA CTAGGAGACT CAGGAGTAAC AGAAATCTCC 
AGTCTACTGG CAAACTCGCA GTGCATGCTG AAGATACTCG GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC 
GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT 
CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT 
GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA 
TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA 
TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG 
GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG 
CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC 
AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA 
GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC 
TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT 
GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA 
GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG 
CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG 
GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG 
AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG 
GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC 
TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG 
AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT 
AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG 
TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA 
ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG 
AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_009298424.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296699.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X6, mRNA.

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CloneID	ODa45493
Clone ID Related Accession (Same CDS sequence)	XM_009298424.2
Accession Version	XM_009298424.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC568664 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298424.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG 
TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA TTTACTGCAG 
GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGACGCCTGT 
CGGTTTGTGA CTAAAATTGT GGGTAAAAAC CCATTACTCC TGACAGAACT GAATCTGAGT 
GATCGTAAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGTAGAGTCA ACACAATAAT GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT 
TTAACTTCAG CATTTATTTT AAACCCATCA CACCTGATAG AGCTCAATCT GAGTGGGAAT 
AAACTAGGAG ACTCAGGAGT AGAGAAGATC TGTCCTCTGC TGGAAAATAC ACAATGCCGA 
TTAGAGAAAC TGAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGAA GCTCACTGAT TTACTGCACA GTAGAAGCTG TAAATTCAGA 
TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA TGTGACTATC TTACTAAAGT TCTGGGTATA 
AGTCCATTAT TACTGACAGA ACTGGATCTG AGTGAAGATA AATTAGGAGA TCTGGATGGA 
GAGAAACTCT CTGCTCTTCT GATGGACTCT CATAGCAAAG TGAAGAAAAT CAAGCTGAAT 
AATTGTGAGC TGACAGAGAA AAGCTGTTCA CTTCTAGCTG CAGTTCTCTC CTCCAAAACC 
ATCCTGAAAG AGATGAACCT GAACAACAGT CGTCTGCTGG ACTCAGGAGT CAGAGAAATC 
TGTGAGGGAC TGAAGAATCC TGTGTGTGAG CTGAAAATAC TCAGTCTTTC AGACTGCCGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAC 
TTACTGGAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGGCTGTC ACTTTGTGAC TGAAATGGTG GGTAAAAACC CATTACTCCT GAAAGAACTG 
AATCTGAGTG ATCGTGTACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTGCTGCAG 
GATAAACACT GTCAGATCCA CACACTCATT CTGTGTTATT GCAGTATTAC AGAGGAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AGTGGAAACA ATCTAGGAGA CTCTGCAGTG AAAAAACTCA GTGATTTACT GATGAACCAA 
CAATTCAAGC TGGAGAAACT ACGTCTGAGT GATTGCAGTA TTACAGAGAA ACAGTGTGTG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAATGGG 
AATAGACTAG GAGACTCTGG AGCTTATGGA CTTGGCCATC TGCTGAGCAG TGTGGATTGC 
AAGCTGAATA AACTACATCT GTGTGATTGC AGTATTACAG GGAAACAGTT GCACTTCCTG 
ATTGCAGTTA TGTGTATGGA CCCATCACAC CTGAGGGTAC TGGACCTCAG TAAGAATCAA 
ATAAAAAGCA GAGGAATGAA CATGCTGTGT GATGTGCTGA ATAAACCACG CTGTAAACTC 
AAGAGTTTAA GACTAAATGA CTGTGGCATT ACAGATGTTA CACACTTAAC TCAGTCTTTG 
GCTGAGAACA AAGCACTGGA GTTCTTAAAA GAGCTTGAAC TGAGTAAAAA TCAAATGGGA 
GACTCCGAAA AGCAAAAACT CAGTGACCTG CTGCGAGACT CAAACTGTAA ACTGAGGTTA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296699.1
CDS	182..6907
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_009298424.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298424.2

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG 
TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGTCTTT CAGACTGCAG TATCAGTGAA 
GAAGGTTATA AAGCTCTGGC TTCAGCTCTG AGATCAAACC CTTCACACCT GATAGAGCTG 
GATCTCAGAG GAAATGATCC TGGACAATCA GGAGTGAAGG AGCTCACTGA TTTACTGCAG 
GATCCACACT GTACACTGAA GACACTGAGG TTTTTGAGTC CTGCTGCTGA TGACGCCTGT 
CGGTTTGTGA CTAAAATTGT GGGTAAAAAC CCATTACTCC TGACAGAACT GAATCTGAGT 
GATCGTAAAC TAGGAGACAC ACGAGTGAAT CAACTCTCTG CTCTACTGCA GGATAAACAC 
TGTAGAGTCA ACACAATAAT GCTGAATAAT AACAGGATTA CAGCAGAAGG TTGTGCTGCT 
TTAACTTCAG CATTTATTTT AAACCCATCA CACCTGATAG AGCTCAATCT GAGTGGGAAT 
AAACTAGGAG ACTCAGGAGT AGAGAAGATC TGTCCTCTGC TGGAAAATAC ACAATGCCGA 
TTAGAGAAAC TGAGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGAA GCTCACTGAT TTACTGCACA GTAGAAGCTG TAAATTCAGA 
TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA TGTGACTATC TTACTAAAGT TCTGGGTATA 
AGTCCATTAT TACTGACAGA ACTGGATCTG AGTGAAGATA AATTAGGAGA TCTGGATGGA 
GAGAAACTCT CTGCTCTTCT GATGGACTCT CATAGCAAAG TGAAGAAAAT CAAGCTGAAT 
AATTGTGAGC TGACAGAGAA AAGCTGTTCA CTTCTAGCTG CAGTTCTCTC CTCCAAAACC 
ATCCTGAAAG AGATGAACCT GAACAACAGT CGTCTGCTGG ACTCAGGAGT CAGAGAAATC 
TGTGAGGGAC TGAAGAATCC TGTGTGTGAG CTGAAAATAC TCAGTCTTTC AGACTGCCGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAC 
TTACTGGAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GAAGGCTGTC ACTTTGTGAC TGAAATGGTG GGTAAAAACC CATTACTCCT GAAAGAACTG 
AATCTGAGTG ATCGTGTACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTGCTGCAG 
GATAAACACT GTCAGATCCA CACACTCATT CTGTGTTATT GCAGTATTAC AGAGGAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AGTGGAAACA ATCTAGGAGA CTCTGCAGTG AAAAAACTCA GTGATTTACT GATGAACCAA 
CAATTCAAGC TGGAGAAACT ACGTCTGAGT GATTGCAGTA TTACAGAGAA ACAGTGTGTG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAATGGG 
AATAGACTAG GAGACTCTGG AGCTTATGGA CTTGGCCATC TGCTGAGCAG TGTGGATTGC 
AAGCTGAATA AACTACATCT GTGTGATTGC AGTATTACAG GGAAACAGTT GCACTTCCTG 
ATTGCAGTTA TGTGTATGGA CCCATCACAC CTGAGGGTAC TGGACCTCAG TAAGAATCAA 
ATAAAAAGCA GAGGAATGAA CATGCTGTGT GATGTGCTGA ATAAACCACG CTGTAAACTC 
AAGAGTTTAA GACTAAATGA CTGTGGCATT ACAGATGTTA CACACTTAAC TCAGTCTTTG 
GCTGAGAACA AAGCACTGGA GTTCTTAAAA GAGCTTGAAC TGAGTAAAAA TCAAATGGGA 
GACTCCGAAA AGCAAAAACT CAGTGACCTG CTGCGAGACT CAAACTGTAA ACTGAGGTTA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_009298425.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296700.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X7, mRNA.

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CloneID	ODa45494
Clone ID Related Accession (Same CDS sequence)	XM_009298425.2
Accession Version	XM_009298425.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC568664 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GACGCCTGTC GGTTTGTGAC TAAAATTGTG GGTAAAAACC CATTACTCCT GACAGAACTG 
AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAGAGTCAA CACAATAATG CTGAATAATA ACAGGATTAC AGCAGAAGGT 
TGTGCTGCTT TAACTTCAGC ATTTATTTTA AACCCATCAC ACCTGATAGA GCTCAATCTG 
AGTGGGAATA AACTAGGAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATACA 
CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGAAG CTCACTGATT TACTGCACAG TAGAAGCTGT 
AAATTCAGAT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GTGACTATCT TACTAAAGTT 
CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT 
CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GAAGAAAATC 
AAGCTGAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC 
TCCAAAACCA TCCTGAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
AGAGAAATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAAATACT CAGTCTTTCA 
GACTGCCGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGACT TACTGGAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG AAGGCTGTCA CTTTGTGACT GAAATGGTGG GTAAAAACCC ATTACTCCTG 
AAAGAACTGA ATCTGAGTGA TCGTGTACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT 
CTGCTGCAGG ATAAACACTG TCAGATCCAC ACACTCATTC TGTGTTATTG CAGTATTACA 
GAGGAACAGT GTGTGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA 
CTGAACCTCA GTGGAAACAA TCTAGGAGAC TCTGCAGTGA AAAAACTCAG TGATTTACTG 
ATGAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTG ATTGCAGTAT TACAGAGAAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTTATGGAC TTGGCCATCT GCTGAGCAGT 
GTGGATTGCA AGCTGAATAA ACTACATCTG TGTGATTGCA GTATTACAGG GAAACAGTTG 
CACTTCCTGA TTGCAGTTAT GTGTATGGAC CCATCACACC TGAGGGTACT GGACCTCAGT 
AAGAATCAAA TAAAAAGCAG AGGAATGAAC ATGCTGTGTG ATGTGCTGAA TAAACCACGC 
TGTAAACTCA AGAGTTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACACTTAACT 
CAGTCTTTGG CTGAGAACAA AGCACTGGAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT 
CAAATGGGAG ACTCCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA 
CTGAGGTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296700.1
CDS	182..6736
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_009298425.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298425.2

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGGA GCTCACTGAT 
TTACTGCAGG ATCCACACTG TACACTGAAG ACACTGAGGT TTTTGAGTCC TGCTGCTGAT 
GACGCCTGTC GGTTTGTGAC TAAAATTGTG GGTAAAAACC CATTACTCCT GACAGAACTG 
AATCTGAGTG ATCGTAAACT AGGAGACACA CGAGTGAATC AACTCTCTGC TCTACTGCAG 
GATAAACACT GTAGAGTCAA CACAATAATG CTGAATAATA ACAGGATTAC AGCAGAAGGT 
TGTGCTGCTT TAACTTCAGC ATTTATTTTA AACCCATCAC ACCTGATAGA GCTCAATCTG 
AGTGGGAATA AACTAGGAGA CTCAGGAGTA GAGAAGATCT GTCCTCTGCT GGAAAATACA 
CAATGCCGAT TAGAGAAACT GAGTCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGAAG CTCACTGATT TACTGCACAG TAGAAGCTGT 
AAATTCAGAT TTCTGAAGAG TTCTGCTGCA CAGGAAGCAT GTGACTATCT TACTAAAGTT 
CTGGGTATAA GTCCATTATT ACTGACAGAA CTGGATCTGA GTGAAGATAA ATTAGGAGAT 
CTGGATGGAG AGAAACTCTC TGCTCTTCTG ATGGACTCTC ATAGCAAAGT GAAGAAAATC 
AAGCTGAATA ATTGTGAGCT GACAGAGAAA AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC 
TCCAAAACCA TCCTGAAAGA GATGAACCTG AACAACAGTC GTCTGCTGGA CTCAGGAGTC 
AGAGAAATCT GTGAGGGACT GAAGAATCCT GTGTGTGAGC TGAAAATACT CAGTCTTTCA 
GACTGCCGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGACT TACTGGAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG AAGGCTGTCA CTTTGTGACT GAAATGGTGG GTAAAAACCC ATTACTCCTG 
AAAGAACTGA ATCTGAGTGA TCGTGTACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT 
CTGCTGCAGG ATAAACACTG TCAGATCCAC ACACTCATTC TGTGTTATTG CAGTATTACA 
GAGGAACAGT GTGTGATCCT GACTTCAGCT CTGAAATCAA ACCCATCACA CCTGAGAGAA 
CTGAACCTCA GTGGAAACAA TCTAGGAGAC TCTGCAGTGA AAAAACTCAG TGATTTACTG 
ATGAACCAAC AATTCAAGCT GGAGAAACTA CGTCTGAGTG ATTGCAGTAT TACAGAGAAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAATGGGA ATAGACTAGG AGACTCTGGA GCTTATGGAC TTGGCCATCT GCTGAGCAGT 
GTGGATTGCA AGCTGAATAA ACTACATCTG TGTGATTGCA GTATTACAGG GAAACAGTTG 
CACTTCCTGA TTGCAGTTAT GTGTATGGAC CCATCACACC TGAGGGTACT GGACCTCAGT 
AAGAATCAAA TAAAAAGCAG AGGAATGAAC ATGCTGTGTG ATGTGCTGAA TAAACCACGC 
TGTAAACTCA AGAGTTTAAG ACTAAATGAC TGTGGCATTA CAGATGTTAC ACACTTAACT 
CAGTCTTTGG CTGAGAACAA AGCACTGGAG TTCTTAAAAG AGCTTGAACT GAGTAAAAAT 
CAAATGGGAG ACTCCGAAAA GCAAAAACTC AGTGACCTGC TGCGAGACTC AAACTGTAAA 
CTGAGGTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_009298426.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296701.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X8, mRNA.

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CloneID	ODa45495
Clone ID Related Accession (Same CDS sequence)	XM_009298426.2
Accession Version	XM_009298426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC568664 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGATT TACTGCAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG ACGCCTGTCG GTTTGTGACT AAAATTGTGG GTAAAAACCC ATTACTCCTG 
ACAGAACTGA ATCTGAGTGA TCGTAAACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT 
CTACTGCAGG ATAAACACTG TAGAGTCAAC ACAATAATGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT AACTTCAGCA TTTATTTTAA ACCCATCACA CCTGATAGAG 
CTCAATCTGA GTGGGAATAA ACTAGGAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG 
GAAAATACAC AATGCCGATT AGAGAAACTG AGTCTTTCAG ACTGCAGTAT CAGTGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGAAGC TCACTGATTT ACTGCACAGT 
AGAAGCTGTA AATTCAGATT TCTGAAGAGT TCTGCTGCAC AGGAAGCATG TGACTATCTT 
ACTAAAGTTC TGGGTATAAG TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGATAAA 
TTAGGAGATC TGGATGGAGA GAAACTCTCT GCTCTTCTGA TGGACTCTCA TAGCAAAGTG 
AAGAAAATCA AGCTGAATAA TTGTGAGCTG ACAGAGAAAA GCTGTTCACT TCTAGCTGCA 
GTTCTCTCCT CCAAAACCAT CCTGAAAGAG ATGAACCTGA ACAACAGTCG TCTGCTGGAC 
TCAGGAGTCA GAGAAATCTG TGAGGGACTG AAGAATCCTG TGTGTGAGCT GAAAATACTC 
AGTCTTTCAG ACTGCCGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGACTT ACTGGAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA AGGCTGTCAC TTTGTGACTG AAATGGTGGG TAAAAACCCA 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTGTACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TGCTGCAGGA TAAACACTGT CAGATCCACA CACTCATTCT GTGTTATTGC 
AGTATTACAG AGGAACAGTG TGTGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC 
CTGAGAGAAC TGAACCTCAG TGGAAACAAT CTAGGAGACT CTGCAGTGAA AAAACTCAGT 
GATTTACTGA TGAACCAACA ATTCAAGCTG GAGAAACTAC GTCTGAGTGA TTGCAGTATT 
ACAGAGAAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAATGGGAA TAGACTAGGA GACTCTGGAG CTTATGGACT TGGCCATCTG 
CTGAGCAGTG TGGATTGCAA GCTGAATAAA CTACATCTGT GTGATTGCAG TATTACAGGG 
AAACAGTTGC ACTTCCTGAT TGCAGTTATG TGTATGGACC CATCACACCT GAGGGTACTG 
GACCTCAGTA AGAATCAAAT AAAAAGCAGA GGAATGAACA TGCTGTGTGA TGTGCTGAAT 
AAACCACGCT GTAAACTCAA GAGTTTAAGA CTAAATGACT GTGGCATTAC AGATGTTACA 
CACTTAACTC AGTCTTTGGC TGAGAACAAA GCACTGGAGT TCTTAAAAGA GCTTGAACTG 
AGTAAAAATC AAATGGGAGA CTCCGAAAAG CAAAAACTCA GTGACCTGCT GCGAGACTCA 
AACTGTAAAC TGAGGTTAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296701.1
CDS	182..6565
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_009298426.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298426.2

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGGAG 
CTCACTGATT TACTGCAGGA TCCACACTGT ACACTGAAGA CACTGAGGTT TTTGAGTCCT 
GCTGCTGATG ACGCCTGTCG GTTTGTGACT AAAATTGTGG GTAAAAACCC ATTACTCCTG 
ACAGAACTGA ATCTGAGTGA TCGTAAACTA GGAGACACAC GAGTGAATCA ACTCTCTGCT 
CTACTGCAGG ATAAACACTG TAGAGTCAAC ACAATAATGC TGAATAATAA CAGGATTACA 
GCAGAAGGTT GTGCTGCTTT AACTTCAGCA TTTATTTTAA ACCCATCACA CCTGATAGAG 
CTCAATCTGA GTGGGAATAA ACTAGGAGAC TCAGGAGTAG AGAAGATCTG TCCTCTGCTG 
GAAAATACAC AATGCCGATT AGAGAAACTG AGTCTTTCAG ACTGCAGTAT CAGTGAAGAA 
GGTTATAAAG CTCTGGCTTC AGCTCTGAGA TCAAACCCTT CACACCTGAT AGAGCTGGAT 
CTCAGAGGAA ATGATCCTGG ACAATCAGGA GTGAAGAAGC TCACTGATTT ACTGCACAGT 
AGAAGCTGTA AATTCAGATT TCTGAAGAGT TCTGCTGCAC AGGAAGCATG TGACTATCTT 
ACTAAAGTTC TGGGTATAAG TCCATTATTA CTGACAGAAC TGGATCTGAG TGAAGATAAA 
TTAGGAGATC TGGATGGAGA GAAACTCTCT GCTCTTCTGA TGGACTCTCA TAGCAAAGTG 
AAGAAAATCA AGCTGAATAA TTGTGAGCTG ACAGAGAAAA GCTGTTCACT TCTAGCTGCA 
GTTCTCTCCT CCAAAACCAT CCTGAAAGAG ATGAACCTGA ACAACAGTCG TCTGCTGGAC 
TCAGGAGTCA GAGAAATCTG TGAGGGACTG AAGAATCCTG TGTGTGAGCT GAAAATACTC 
AGTCTTTCAG ACTGCCGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGACTT ACTGGAGGAT CCACACTGTA CACTGAAGAC ACTGAGGTTT 
TTGAGTCCTG CTGCTGATGA AGGCTGTCAC TTTGTGACTG AAATGGTGGG TAAAAACCCA 
TTACTCCTGA AAGAACTGAA TCTGAGTGAT CGTGTACTAG GAGACACACG AGTGAATCAA 
CTCTCTGCTC TGCTGCAGGA TAAACACTGT CAGATCCACA CACTCATTCT GTGTTATTGC 
AGTATTACAG AGGAACAGTG TGTGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC 
CTGAGAGAAC TGAACCTCAG TGGAAACAAT CTAGGAGACT CTGCAGTGAA AAAACTCAGT 
GATTTACTGA TGAACCAACA ATTCAAGCTG GAGAAACTAC GTCTGAGTGA TTGCAGTATT 
ACAGAGAAAC AGTGTGTGAT CCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGAACC TCAATGGGAA TAGACTAGGA GACTCTGGAG CTTATGGACT TGGCCATCTG 
CTGAGCAGTG TGGATTGCAA GCTGAATAAA CTACATCTGT GTGATTGCAG TATTACAGGG 
AAACAGTTGC ACTTCCTGAT TGCAGTTATG TGTATGGACC CATCACACCT GAGGGTACTG 
GACCTCAGTA AGAATCAAAT AAAAAGCAGA GGAATGAACA TGCTGTGTGA TGTGCTGAAT 
AAACCACGCT GTAAACTCAA GAGTTTAAGA CTAAATGACT GTGGCATTAC AGATGTTACA 
CACTTAACTC AGTCTTTGGC TGAGAACAAA GCACTGGAGT TCTTAAAAGA GCTTGAACTG 
AGTAAAAATC AAATGGGAGA CTCCGAAAAG CAAAAACTCA GTGACCTGCT GCGAGACTCA 
AACTGTAAAC TGAGGTTAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_009298427.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296702.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X10, mRNA.

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CloneID	ODa45496
Clone ID Related Accession (Same CDS sequence)	XM_009298427.2
Accession Version	XM_009298427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC568664 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAT TTTAAACCCA 
TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAGAGAAG 
ATCTGTCCTC TGCTGGAAAA TACACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG 
AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG 
GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC 
ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA 
GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT 
GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA 
CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG 
TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296702.1
CDS	182..6220
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_009298427.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298427.2

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAT TTTAAACCCA 
TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAGAGAAG 
ATCTGTCCTC TGCTGGAAAA TACACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG 
AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG 
GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC 
ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA 
GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT 
GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA 
CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG 
TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_009298428.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296703.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X11, mRNA.

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CloneID	ODa45497
Clone ID Related Accession (Same CDS sequence)	XM_009298428.2
Accession Version	XM_009298428.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC568664 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAATC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGACGCC TGTCGGTTTG TGACTAAAAT TGTGGGTAAA 
AACCCATTAC TCCTGACAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAGAG TCAACACAAT AATGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAT TTTAAACCCA 
TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAGAGAAG 
ATCTGTCCTC TGCTGGAAAA TACACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG 
AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG 
GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC 
ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA 
GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT 
GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA 
CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG 
TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296703.1
CDS	182..6220
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_009298428.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298428.2

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAATC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGATTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGACGCC TGTCGGTTTG TGACTAAAAT TGTGGGTAAA 
AACCCATTAC TCCTGACAGA ACTGAATCTG AGTGATCGTA AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAGAG TCAACACAAT AATGCTGAAT 
AATAACAGGA TTACAGCAGA AGGTTGTGCT GCTTTAACTT CAGCATTTAT TTTAAACCCA 
TCACACCTGA TAGAGCTCAA TCTGAGTGGG AATAAACTAG GAGACTCAGG AGTAGAGAAG 
ATCTGTCCTC TGCTGGAAAA TACACAATGC CGATTAGAGA AACTGAGTCT TTCAGACTGC 
AGTATCAGTG AAGAAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCCTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GAAGCTCACT 
GATTTACTGC ACAGTAGAAG CTGTAAATTC AGATTTCTGA AGAGTTCTGC TGCACAGGAA 
GCATGTGACT ATCTTACTAA AGTTCTGGGT ATAAGTCCAT TATTACTGAC AGAACTGGAT 
CTGAGTGAAG ATAAATTAGG AGATCTGGAT GGAGAGAAAC TCTCTGCTCT TCTGATGGAC 
TCTCATAGCA AAGTGAAGAA AATCAAGCTG AATAATTGTG AGCTGACAGA GAAAAGCTGT 
TCACTTCTAG CTGCAGTTCT CTCCTCCAAA ACCATCCTGA AAGAGATGAA CCTGAACAAC 
AGTCGTCTGC TGGACTCAGG AGTCAGAGAA ATCTGTGAGG GACTGAAGAA TCCTGTGTGT 
GAGCTGAAAA TACTCAGTCT TTCAGACTGC CGTATCAGTG AAGAAGGTTA TAAAGCTCTG 
GCTTCAGCTC TGAGATCAAA CCCTTCACAC CTGATAGAGC TGGATCTCAG AGGAAATGAT 
CCTGGACAAT CAGGAGTGAA GGAGCTCACT GACTTACTGG AGGATCCACA CTGTACACTG 
AAGACACTGA GGTTTTTGAG TCCTGCTGCT GATGAAGGCT GTCACTTTGT GACTGAAATG 
GTGGGTAAAA ACCCATTACT CCTGAAAGAA CTGAATCTGA GTGATCGTGT ACTAGGAGAC 
ACACGAGTGA ATCAACTCTC TGCTCTGCTG CAGGATAAAC ACTGTCAGAT CCACACACTC 
ATTCTGTGTT ATTGCAGTAT TACAGAGGAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAGTGGAA ACAATCTAGG AGACTCTGCA 
GTGAAAAAAC TCAGTGATTT ACTGATGAAC CAACAATTCA AGCTGGAGAA ACTACGTCTG 
AGTGATTGCA GTATTACAGA GAAACAGTGT GTGATCCTGA CTTCAGCTCT GAAATCAAAC 
CCATCACACC TGAGAGAACT GAACCTCAAT GGGAATAGAC TAGGAGACTC TGGAGCTTAT 
GGACTTGGCC ATCTGCTGAG CAGTGTGGAT TGCAAGCTGA ATAAACTACA TCTGTGTGAT 
TGCAGTATTA CAGGGAAACA GTTGCACTTC CTGATTGCAG TTATGTGTAT GGACCCATCA 
CACCTGAGGG TACTGGACCT CAGTAAGAAT CAAATAAAAA GCAGAGGAAT GAACATGCTG 
TGTGATGTGC TGAATAAACC ACGCTGTAAA CTCAAGAGTT TAAGACTAAA TGACTGTGGC 
ATTACAGATG TTACACACTT AACTCAGTCT TTGGCTGAGA ACAAAGCACT GGAGTTCTTA 
AAAGAGCTTG AACTGAGTAA AAATCAAATG GGAGACTCCG AAAAGCAAAA ACTCAGTGAC 
CTGCTGCGAG ACTCAAACTG TAAACTGAGG TTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_009298429.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296704.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X12, mRNA.

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CloneID	ODa45498
Clone ID Related Accession (Same CDS sequence)	XM_009298429.2
Accession Version	XM_009298429.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC568664 isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAT 
TTACTGCACA GTAGAAGCTG TAAATTCAGA TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA 
TGTGACTATC TTACTAAAGT TCTGGGTATA AGTCCATTAT TACTGACAGA ACTGGATCTG 
AGTGAAGATA AATTAGGAGA TCTGGATGGA GAGAAACTCT CTGCTCTTCT GATGGACTCT 
CATAGCAAAG TGAAGAAAAT CAAGCTGAAT AATTGTGAGC TGACAGAGAA AAGCTGTTCA 
CTTCTAGCTG CAGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT 
CGTCTGCTGG ACTCAGGAGT CAGAGAAATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG 
CTGAAAATAC TCAGTCTTTC AGACTGCCGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGGAGG ATCCACACTG TACACTGAAG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAAGGCTGTC ACTTTGTGAC TGAAATGGTG 
GGTAAAAACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGTACT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTGCTGCAG GATAAACACT GTCAGATCCA CACACTCATT 
CTGTGTTATT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGAAACA ATCTAGGAGA CTCTGCAGTG 
AAAAAACTCA GTGATTTACT GATGAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT 
GATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTTATGGA 
CTTGGCCATC TGCTGAGCAG TGTGGATTGC AAGCTGAATA AACTACATCT GTGTGATTGC 
AGTATTACAG GGAAACAGTT GCACTTCCTG ATTGCAGTTA TGTGTATGGA CCCATCACAC 
CTGAGGGTAC TGGACCTCAG TAAGAATCAA ATAAAAAGCA GAGGAATGAA CATGCTGTGT 
GATGTGCTGA ATAAACCACG CTGTAAACTC AAGAGTTTAA GACTAAATGA CTGTGGCATT 
ACAGATGTTA CACACTTAAC TCAGTCTTTG GCTGAGAACA AAGCACTGGA GTTCTTAAAA 
GAGCTTGAAC TGAGTAAAAA TCAAATGGGA GACTCCGAAA AGCAAAAACT CAGTGACCTG 
CTGCGAGACT CAAACTGTAA ACTGAGGTTA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296704.1
CDS	182..6217
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_009298429.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298429.2

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTC AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAT 
TTACTGCACA GTAGAAGCTG TAAATTCAGA TTTCTGAAGA GTTCTGCTGC ACAGGAAGCA 
TGTGACTATC TTACTAAAGT TCTGGGTATA AGTCCATTAT TACTGACAGA ACTGGATCTG 
AGTGAAGATA AATTAGGAGA TCTGGATGGA GAGAAACTCT CTGCTCTTCT GATGGACTCT 
CATAGCAAAG TGAAGAAAAT CAAGCTGAAT AATTGTGAGC TGACAGAGAA AAGCTGTTCA 
CTTCTAGCTG CAGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT 
CGTCTGCTGG ACTCAGGAGT CAGAGAAATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG 
CTGAAAATAC TCAGTCTTTC AGACTGCCGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAC TTACTGGAGG ATCCACACTG TACACTGAAG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAAGGCTGTC ACTTTGTGAC TGAAATGGTG 
GGTAAAAACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGTACT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTGCTGCAG GATAAACACT GTCAGATCCA CACACTCATT 
CTGTGTTATT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGAAACA ATCTAGGAGA CTCTGCAGTG 
AAAAAACTCA GTGATTTACT GATGAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT 
GATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTTATGGA 
CTTGGCCATC TGCTGAGCAG TGTGGATTGC AAGCTGAATA AACTACATCT GTGTGATTGC 
AGTATTACAG GGAAACAGTT GCACTTCCTG ATTGCAGTTA TGTGTATGGA CCCATCACAC 
CTGAGGGTAC TGGACCTCAG TAAGAATCAA ATAAAAAGCA GAGGAATGAA CATGCTGTGT 
GATGTGCTGA ATAAACCACG CTGTAAACTC AAGAGTTTAA GACTAAATGA CTGTGGCATT 
ACAGATGTTA CACACTTAAC TCAGTCTTTG GCTGAGAACA AAGCACTGGA GTTCTTAAAA 
GAGCTTGAAC TGAGTAAAAA TCAAATGGGA GACTCCGAAA AGCAAAAACT CAGTGACCTG 
CTGCGAGACT CAAACTGTAA ACTGAGGTTA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_009298432.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296707.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X20, mRNA.

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CloneID	ODa45501
Clone ID Related Accession (Same CDS sequence)	XM_009298432.2
Accession Version	XM_009298432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 12 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298432.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC 
GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT 
CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT 
GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA 
TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA 
TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG 
GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG 
CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC 
AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA 
GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC 
TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT 
GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA 
GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG 
CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG 
GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG 
AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG 
GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC 
TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG 
AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT 
AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG 
TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA 
ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG 
AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296707.1
CDS	182..5353
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_009298432.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298432.2

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GTCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CTGCAGGATC CACACTGTAC ACTGAAGACA CTGAGGTTTT TGAGTCCTGC TGCTGATGAC 
GCCTGTCGGT TTGTGACTAA AATTGTGGGT AAAAACCCAT TACTCCTGAC AGAACTGAAT 
CTGAGTGATC GTAAACTAGG AGACACACGA GTGAATCAAC TCTCTGCTCT ACTGCAGGAT 
AAACACTGTA GAGTCAACAC AATAATGCTG AATAATAACA GGATTACAGC AGAAGGTTGT 
GCTGCTTTAA CTTCAGCATT TATTTTAAAC CCATCACACC TGATAGAGCT CAATCTGAGT 
GGGAATAAAC TAGGAGACTC AGGAGTAGAG AAGATCTGTC CTCTGCTGGA AAATACACAA 
TGCCGATTAG AGAAACTGAG TCTTTCAGAC TGCAGTATCA GTGAAGAAGG TTATAAAGCT 
CTGGCTTCAG CTCTGAGATC AAACCCTTCA CACCTGATAG AGCTGGATCT CAGAGGAAAT 
GATCCTGGAC AATCAGGAGT GAAGAAGCTC ACTGATTTAC TGCACAGTAG AAGCTGTAAA 
TTCAGATTTC TGAAGAGTTC TGCTGCACAG GAAGCATGTG ACTATCTTAC TAAAGTTCTG 
GGTATAAGTC CATTATTACT GACAGAACTG GATCTGAGTG AAGATAAATT AGGAGATCTG 
GATGGAGAGA AACTCTCTGC TCTTCTGATG GACTCTCATA GCAAAGTGAA GAAAATCAAG 
CTGAATAATT GTGAGCTGAC AGAGAAAAGC TGTTCACTTC TAGCTGCAGT TCTCTCCTCC 
AAAACCATCC TGAAAGAGAT GAACCTGAAC AACAGTCGTC TGCTGGACTC AGGAGTCAGA 
GAAATCTGTG AGGGACTGAA GAATCCTGTG TGTGAGCTGA AAATACTCAG TCTTTCAGAC 
TGCCGTATCA GTGAAGAAGG TTATAAAGCT CTGGCTTCAG CTCTGAGATC AAACCCTTCA 
CACCTGATAG AGCTGGATCT CAGAGGAAAT GATCCTGGAC AATCAGGAGT GAAGGAGCTC 
ACTGACTTAC TGGAGGATCC ACACTGTACA CTGAAGACAC TGAGGTTTTT GAGTCCTGCT 
GCTGATGAAG GCTGTCACTT TGTGACTGAA ATGGTGGGTA AAAACCCATT ACTCCTGAAA 
GAACTGAATC TGAGTGATCG TGTACTAGGA GACACACGAG TGAATCAACT CTCTGCTCTG 
CTGCAGGATA AACACTGTCA GATCCACACA CTCATTCTGT GTTATTGCAG TATTACAGAG 
GAACAGTGTG TGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAGTG GAAACAATCT AGGAGACTCT GCAGTGAAAA AACTCAGTGA TTTACTGATG 
AACCAACAAT TCAAGCTGGA GAAACTACGT CTGAGTGATT GCAGTATTAC AGAGAAACAG 
TGTGTGATCC TGACTTCAGC TCTGAAATCA AACCCATCAC ACCTGAGAGA ACTGAACCTC 
AATGGGAATA GACTAGGAGA CTCTGGAGCT TATGGACTTG GCCATCTGCT GAGCAGTGTG 
GATTGCAAGC TGAATAAACT ACATCTGTGT GATTGCAGTA TTACAGGGAA ACAGTTGCAC 
TTCCTGATTG CAGTTATGTG TATGGACCCA TCACACCTGA GGGTACTGGA CCTCAGTAAG 
AATCAAATAA AAAGCAGAGG AATGAACATG CTGTGTGATG TGCTGAATAA ACCACGCTGT 
AAACTCAAGA GTTTAAGACT AAATGACTGT GGCATTACAG ATGTTACACA CTTAACTCAG 
TCTTTGGCTG AGAACAAAGC ACTGGAGTTC TTAAAAGAGC TTGAACTGAG TAAAAATCAA 
ATGGGAGACT CCGAAAAGCA AAAACTCAGT GACCTGCTGC GAGACTCAAA CTGTAAACTG 
AGGTTAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_009298433.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296708.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ODa45502
Clone ID Related Accession (Same CDS sequence)	XM_009298433.2
Accession Version	XM_009298433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298433.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT GAGCTGACAG AGAAAAGCTG TTCACTTCTA 
GCTGCAGTTC TCTCCTCCAA AACCATCCTG AAAGAGATGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA AATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAA 
ATACTCAGTC TTTCAGACTG CCGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGACTTACTG GAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCACTTTG TGACTGAAAT GGTGGGTAAA 
AACCCATTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTG TACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTGCT GCAGGATAAA CACTGTCAGA TCCACACACT CATTCTGTGT 
TATTGCAGTA TTACAGAGGA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAGTGGA AACAATCTAG GAGACTCTGC AGTGAAAAAA 
CTCAGTGATT TACTGATGAA CCAACAATTC AAGCTGGAGA AACTACGTCT GAGTGATTGC 
AGTATTACAG AGAAACAGTG TGTGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC 
CTGAGAGAAC TGAACCTCAA TGGGAATAGA CTAGGAGACT CTGGAGCTTA TGGACTTGGC 
CATCTGCTGA GCAGTGTGGA TTGCAAGCTG AATAAACTAC ATCTGTGTGA TTGCAGTATT 
ACAGGGAAAC AGTTGCACTT CCTGATTGCA GTTATGTGTA TGGACCCATC ACACCTGAGG 
GTACTGGACC TCAGTAAGAA TCAAATAAAA AGCAGAGGAA TGAACATGCT GTGTGATGTG 
CTGAATAAAC CACGCTGTAA ACTCAAGAGT TTAAGACTAA ATGACTGTGG CATTACAGAT 
GTTACACACT TAACTCAGTC TTTGGCTGAG AACAAAGCAC TGGAGTTCTT AAAAGAGCTT 
GAACTGAGTA AAAATCAAAT GGGAGACTCC GAAAAGCAAA AACTCAGTGA CCTGCTGCGA 
GACTCAAACT GTAAACTGAG GTTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296708.1
CDS	182..5191
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_009298433.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298433.2

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT GAGCTGACAG AGAAAAGCTG TTCACTTCTA 
GCTGCAGTTC TCTCCTCCAA AACCATCCTG AAAGAGATGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA AATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAA 
ATACTCAGTC TTTCAGACTG CCGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGACTTACTG GAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCACTTTG TGACTGAAAT GGTGGGTAAA 
AACCCATTAC TCCTGAAAGA ACTGAATCTG AGTGATCGTG TACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTGCT GCAGGATAAA CACTGTCAGA TCCACACACT CATTCTGTGT 
TATTGCAGTA TTACAGAGGA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAGTGGA AACAATCTAG GAGACTCTGC AGTGAAAAAA 
CTCAGTGATT TACTGATGAA CCAACAATTC AAGCTGGAGA AACTACGTCT GAGTGATTGC 
AGTATTACAG AGAAACAGTG TGTGATCCTG ACTTCAGCTC TGAAATCAAA CCCATCACAC 
CTGAGAGAAC TGAACCTCAA TGGGAATAGA CTAGGAGACT CTGGAGCTTA TGGACTTGGC 
CATCTGCTGA GCAGTGTGGA TTGCAAGCTG AATAAACTAC ATCTGTGTGA TTGCAGTATT 
ACAGGGAAAC AGTTGCACTT CCTGATTGCA GTTATGTGTA TGGACCCATC ACACCTGAGG 
GTACTGGACC TCAGTAAGAA TCAAATAAAA AGCAGAGGAA TGAACATGCT GTGTGATGTG 
CTGAATAAAC CACGCTGTAA ACTCAAGAGT TTAAGACTAA ATGACTGTGG CATTACAGAT 
GTTACACACT TAACTCAGTC TTTGGCTGAG AACAAAGCAC TGGAGTTCTT AAAAGAGCTT 
GAACTGAGTA AAAATCAAAT GGGAGACTCC GAAAAGCAAA AACTCAGTGA CCTGCTGCGA 
GACTCAAACT GTAAACTGAG GTTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_009298436.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296711.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ODa45505
Clone ID Related Accession (Same CDS sequence)	XM_009298436.1
Accession Version	XM_009298436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC568664 isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTC ATAGCAAAGT GGAGAAAATC AAGCTGAATA ATTGTAAGCT GCTTGAGAAA 
AGCTCATCAG TTTTAGCAAC AGTTCTGTCT TCCAAAACCA TCCTGAAAGA GCTGAACCTG 
AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAGAGATCT GTGAGGGACT GAAGAATCCT 
GTGTGTGAGC TGAAGATACT CAAGCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGAGT TACTGCAGGA TCCACACTGT 
ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AAGGCTGTCA GTTTGTGACT 
GGAATTGTGG GTAAAAACCC ATTACTTCTG AAAGAGCTAA ATCTGAGTGA TCGTGAACTA 
GGAGACACAC GAGTGAATCA ACTCTCTGCT CTACTGCAGG ATAAACACTG TAAACTCAAC 
ACACTCATTC TTAGTAAATG TGGTGTAACA GTGGAAAGCT GTTCAGCTCT GGCTACAGTC 
CTGAGATCAG AGACCAGTCT AAAAGAGCTT GACATGAGCA ACAATAATCT GCAGGAATCA 
GGAGTGAAGA AACTCCAGAG CGGATTAAAA AACACAAACT GCACACTGGA AAAACTCAGT 
CTTTTAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG 
AAGAAGCTCA CTGAGTTACT GAAACCAGAC TATACACTGA AGACACTGAG GTTTTTGAGT 
GATGATGCAG ATGAAGCCTG TACACATCTG AATAAAGTTC TGGGTGGAAA CCTGTTACTC 
TTGAGAGAGC TGAATCTGAG TAAACTTGAT CTTGGACCAT CAGGATTGGA GAAACTTGCT 
GCTGTACTGA AAGATAAACA CTGTAAACTC AACACACTGA TTCTGAATAA CAGCAGTATG 
TCAGAAGAAG ATTGTCGTGT TTTGGCTGAA GCTTTTAATT CAAACCCATC AAATCTGACA 
GAGCTGAATT TGAGTGGGAC TAAACTCACA AACTCAGGAA TGAAGAGCTT CACCACTCTG 
TTTCAAAACC AACAGTGTAG ACTGGAAAAG CTGAAGTTCA GCAGCATGTG TATTACAGCA 
GAAGGTTGTG CTGCTTTGGC TTCAGCATTT AACTCAAATC CTTCACACCT GATAGAGCTC 
AATCTGAGTG AGAATAAACT AGGAGACTCA GGAGTAACAG AAATCTCCAG TCTACTGGCA 
AACTCGCAGT GCATGCTGAA GATACTCGGT CTTTCAGACT GCAGTATCAG TGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTCA CTGATTTACT GCAGGATCCA 
CACTGTACAC TGAAGACACT GAGGTTTTTG AGTCCTGCTG CTGATGACGC CTGTCGGTTT 
GTGACTAAAA TTGTGGGTAA AAACCCATTA CTCCTGACAG AACTGAATCT GAGTGATCGT 
AAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTAGA 
GTCAACACAA TAATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTAACT 
TCAGCATTTA TTTTAAACCC ATCACACCTG ATAGAGCTCA ATCTGAGTGG GAATAAACTA 
GGAGACTCAG GAGTAGAGAA GATCTGTCCT CTGCTGGAAA ATACACAATG CCGATTAGAG 
AAACTGAGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGAAGCTCAC TGATTTACTG CACAGTAGAA GCTGTAAATT CAGATTTCTG 
AAGAGTTCTG CTGCACAGGA AGCATGTGAC TATCTTACTA AAGTTCTGGG TATAAGTCCA 
TTATTACTGA CAGAACTGGA TCTGAGTGAA GATAAATTAG GAGATCTGGA TGGAGAGAAA 
CTCTCTGCTC TTCTGATGGA CTCTCATAGC AAAGTGAAGA AAATCAAGCT GAATAATTGT 
GAGCTGACAG AGAAAAGCTG TTCACTTCTA GCTGCAGTTC TCTCCTCCAA AACCATCCTG 
AAAGAGATGA ACCTGAACAA CAGTCGTCTG CTGGACTCAG GAGTCAGAGA AATCTGTGAG 
GGACTGAAGA ATCCTGTGTG TGAGCTGAAA ATACTCAGTC TTTCAGACTG CCGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGACTTACTG 
GAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGAAGGC 
TGTCACTTTG TGACTGAAAT GGTGGGTAAA AACCCATTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCGTG TACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTGCT GCAGGATAAA 
CACTGTCAGA TCCACACACT CATTCTGTGT TATTGCAGTA TTACAGAGGA ACAGTGTGTG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGGA 
AACAATCTAG GAGACTCTGC AGTGAAAAAA CTCAGTGATT TACTGATGAA CCAACAATTC 
AAGCTGGAGA AACTACGTCT GAGTGATTGC AGTATTACAG AGAAACAGTG TGTGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAA TGGGAATAGA 
CTAGGAGACT CTGGAGCTTA TGGACTTGGC CATCTGCTGA GCAGTGTGGA TTGCAAGCTG 
AATAAACTAC ATCTGTGTGA TTGCAGTATT ACAGGGAAAC AGTTGCACTT CCTGATTGCA 
GTTATGTGTA TGGACCCATC ACACCTGAGG GTACTGGACC TCAGTAAGAA TCAAATAAAA 
AGCAGAGGAA TGAACATGCT GTGTGATGTG CTGAATAAAC CACGCTGTAA ACTCAAGAGT 
TTAAGACTAA ATGACTGTGG CATTACAGAT GTTACACACT TAACTCAGTC TTTGGCTGAG 
AACAAAGCAC TGGAGTTCTT AAAAGAGCTT GAACTGAGTA AAAATCAAAT GGGAGACTCC 
GAAAAGCAAA AACTCAGTGA CCTGCTGCGA GACTCAAACT GTAAACTGAG GTTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296711.1
CDS	139..3618
Translation	MDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_009298436.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298436.1

ORF Insert Sequence:

ATGGACCCTC ATAGCAAAGT GGAGAAAATC AAGCTGAATA ATTGTAAGCT GCTTGAGAAA 
AGCTCATCAG TTTTAGCAAC AGTTCTGTCT TCCAAAACCA TCCTGAAAGA GCTGAACCTG 
AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAGAGATCT GTGAGGGACT GAAGAATCCT 
GTGTGTGAGC TGAAGATACT CAAGCTTTCA GACTGCAGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGAGT TACTGCAGGA TCCACACTGT 
ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AAGGCTGTCA GTTTGTGACT 
GGAATTGTGG GTAAAAACCC ATTACTTCTG AAAGAGCTAA ATCTGAGTGA TCGTGAACTA 
GGAGACACAC GAGTGAATCA ACTCTCTGCT CTACTGCAGG ATAAACACTG TAAACTCAAC 
ACACTCATTC TTAGTAAATG TGGTGTAACA GTGGAAAGCT GTTCAGCTCT GGCTACAGTC 
CTGAGATCAG AGACCAGTCT AAAAGAGCTT GACATGAGCA ACAATAATCT GCAGGAATCA 
GGAGTGAAGA AACTCCAGAG CGGATTAAAA AACACAAACT GCACACTGGA AAAACTCAGT 
CTTTTAGACT GCAGTATCAG TGAAGAAGGT TATAAAGCTC TGGCTTCAGC TCTGAGATCA 
AACCCTTCAC ACCTGATAGA GCTGGATCTC AGAGGAAATG ATCCTGGACA ATCAGGAGTG 
AAGAAGCTCA CTGAGTTACT GAAACCAGAC TATACACTGA AGACACTGAG GTTTTTGAGT 
GATGATGCAG ATGAAGCCTG TACACATCTG AATAAAGTTC TGGGTGGAAA CCTGTTACTC 
TTGAGAGAGC TGAATCTGAG TAAACTTGAT CTTGGACCAT CAGGATTGGA GAAACTTGCT 
GCTGTACTGA AAGATAAACA CTGTAAACTC AACACACTGA TTCTGAATAA CAGCAGTATG 
TCAGAAGAAG ATTGTCGTGT TTTGGCTGAA GCTTTTAATT CAAACCCATC AAATCTGACA 
GAGCTGAATT TGAGTGGGAC TAAACTCACA AACTCAGGAA TGAAGAGCTT CACCACTCTG 
TTTCAAAACC AACAGTGTAG ACTGGAAAAG CTGAAGTTCA GCAGCATGTG TATTACAGCA 
GAAGGTTGTG CTGCTTTGGC TTCAGCATTT AACTCAAATC CTTCACACCT GATAGAGCTC 
AATCTGAGTG AGAATAAACT AGGAGACTCA GGAGTAACAG AAATCTCCAG TCTACTGGCA 
AACTCGCAGT GCATGCTGAA GATACTCGGT CTTTCAGACT GCAGTATCAG TGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGAGATCA AACCCTTCAC ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAGCTCA CTGATTTACT GCAGGATCCA 
CACTGTACAC TGAAGACACT GAGGTTTTTG AGTCCTGCTG CTGATGACGC CTGTCGGTTT 
GTGACTAAAA TTGTGGGTAA AAACCCATTA CTCCTGACAG AACTGAATCT GAGTGATCGT 
AAACTAGGAG ACACACGAGT GAATCAACTC TCTGCTCTAC TGCAGGATAA ACACTGTAGA 
GTCAACACAA TAATGCTGAA TAATAACAGG ATTACAGCAG AAGGTTGTGC TGCTTTAACT 
TCAGCATTTA TTTTAAACCC ATCACACCTG ATAGAGCTCA ATCTGAGTGG GAATAAACTA 
GGAGACTCAG GAGTAGAGAA GATCTGTCCT CTGCTGGAAA ATACACAATG CCGATTAGAG 
AAACTGAGTC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGAAGCTCAC TGATTTACTG CACAGTAGAA GCTGTAAATT CAGATTTCTG 
AAGAGTTCTG CTGCACAGGA AGCATGTGAC TATCTTACTA AAGTTCTGGG TATAAGTCCA 
TTATTACTGA CAGAACTGGA TCTGAGTGAA GATAAATTAG GAGATCTGGA TGGAGAGAAA 
CTCTCTGCTC TTCTGATGGA CTCTCATAGC AAAGTGAAGA AAATCAAGCT GAATAATTGT 
GAGCTGACAG AGAAAAGCTG TTCACTTCTA GCTGCAGTTC TCTCCTCCAA AACCATCCTG 
AAAGAGATGA ACCTGAACAA CAGTCGTCTG CTGGACTCAG GAGTCAGAGA AATCTGTGAG 
GGACTGAAGA ATCCTGTGTG TGAGCTGAAA ATACTCAGTC TTTCAGACTG CCGTATCAGT 
GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT CTGAGATCAA ACCCTTCACA CCTGATAGAG 
CTGGATCTCA GAGGAAATGA TCCTGGACAA TCAGGAGTGA AGGAGCTCAC TGACTTACTG 
GAGGATCCAC ACTGTACACT GAAGACACTG AGGTTTTTGA GTCCTGCTGC TGATGAAGGC 
TGTCACTTTG TGACTGAAAT GGTGGGTAAA AACCCATTAC TCCTGAAAGA ACTGAATCTG 
AGTGATCGTG TACTAGGAGA CACACGAGTG AATCAACTCT CTGCTCTGCT GCAGGATAAA 
CACTGTCAGA TCCACACACT CATTCTGTGT TATTGCAGTA TTACAGAGGA ACAGTGTGTG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGGA 
AACAATCTAG GAGACTCTGC AGTGAAAAAA CTCAGTGATT TACTGATGAA CCAACAATTC 
AAGCTGGAGA AACTACGTCT GAGTGATTGC AGTATTACAG AGAAACAGTG TGTGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGAACCTCAA TGGGAATAGA 
CTAGGAGACT CTGGAGCTTA TGGACTTGGC CATCTGCTGA GCAGTGTGGA TTGCAAGCTG 
AATAAACTAC ATCTGTGTGA TTGCAGTATT ACAGGGAAAC AGTTGCACTT CCTGATTGCA 
GTTATGTGTA TGGACCCATC ACACCTGAGG GTACTGGACC TCAGTAAGAA TCAAATAAAA 
AGCAGAGGAA TGAACATGCT GTGTGATGTG CTGAATAAAC CACGCTGTAA ACTCAAGAGT 
TTAAGACTAA ATGACTGTGG CATTACAGAT GTTACACACT TAACTCAGTC TTTGGCTGAG 
AACAAAGCAC TGGAGTTCTT AAAAGAGCTT GAACTGAGTA AAAATCAAAT GGGAGACTCC 
GAAAAGCAAA AACTCAGTGA CCTGCTGCGA GACTCAAACT GTAAACTGAG GTTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_017354578.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210067.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X2, mRNA.

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CloneID	ODa53641
Clone ID Related Accession (Same CDS sequence)	XM_017354578.1
Accession Version	XM_017354578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC568664 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG 
TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA 
GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT 
CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT 
CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG 
TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT 
TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT 
AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG 
CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GACGCCTGTC GGTTTGTGAC TAAAATTGTG 
GGTAAAAACC CATTACTCCT GACAGAACTG AATCTGAGTG ATCGTAAACT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GTAGAGTCAA CACAATAATG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTATTTTA 
AACCCATCAC ACCTGATAGA GCTCAATCTG AGTGGGAATA AACTAGGAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GGAAAATACA CAATGCCGAT TAGAGAAACT GAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGAAG 
CTCACTGATT TACTGCACAG TAGAAGCTGT AAATTCAGAT TTCTGAAGAG TTCTGCTGCA 
CAGGAAGCAT GTGACTATCT TACTAAAGTT CTGGGTATAA GTCCATTATT ACTGACAGAA 
CTGGATCTGA GTGAAGATAA ATTAGGAGAT CTGGATGGAG AGAAACTCTC TGCTCTTCTG 
ATGGACTCTC ATAGCAAAGT GAAGAAAATC AAGCTGAATA ATTGTGAGCT GACAGAGAAA 
AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC TCCAAAACCA TCCTGAAAGA GATGAACCTG 
AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAGAAATCT GTGAGGGACT GAAGAATCCT 
GTGTGTGAGC TGAAAATACT CAGTCTTTCA GACTGCCGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGACT TACTGGAGGA TCCACACTGT 
ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AAGGCTGTCA CTTTGTGACT 
GAAATGGTGG GTAAAAACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGTACTA 
GGAGACACAC GAGTGAATCA ACTCTCTGCT CTGCTGCAGG ATAAACACTG TCAGATCCAC 
ACACTCATTC TGTGTTATTG CAGTATTACA GAGGAACAGT GTGTGATCCT GACTTCAGCT 
CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGAAACAA TCTAGGAGAC 
TCTGCAGTGA AAAAACTCAG TGATTTACTG ATGAACCAAC AATTCAAGCT GGAGAAACTA 
CGTCTGAGTG ATTGCAGTAT TACAGAGAAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAATGGGA ATAGACTAGG AGACTCTGGA 
GCTTATGGAC TTGGCCATCT GCTGAGCAGT GTGGATTGCA AGCTGAATAA ACTACATCTG 
TGTGATTGCA GTATTACAGG GAAACAGTTG CACTTCCTGA TTGCAGTTAT GTGTATGGAC 
CCATCACACC TGAGGGTACT GGACCTCAGT AAGAATCAAA TAAAAAGCAG AGGAATGAAC 
ATGCTGTGTG ATGTGCTGAA TAAACCACGC TGTAAACTCA AGAGTTTAAG ACTAAATGAC 
TGTGGCATTA CAGATGTTAC ACACTTAACT CAGTCTTTGG CTGAGAACAA AGCACTGGAG 
TTCTTAAAAG AGCTTGAACT GAGTAAAAAT CAAATGGGAG ACTCCGAAAA GCAAAAACTC 
AGTGACCTGC TGCGAGACTC AAACTGTAAA CTGAGGTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210067.1
CDS	182..7426
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADDACRFVTKIVGKNPLLLTELNLSDRKLGDTRVNQLSALLQDKHCRVNTIMLNNNRITAEGCAALTSAFILNPSHLIELNLSGNKLGDSGVEKICPLLENTQCRLEKLSLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTDLLHSRSCKFRFLKSSAAQEACDYLTKVLGISPLLLTELDLSEDKLGDLDGEKLSALLMDSHSKVKKIKLNNCELTEKSCSLLAAVLSSKTILKEMNLNNSRLLDSGVREICEGLKNPVCELKILSLSDCRISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLEDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_017354578.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354578.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG 
TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA 
GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT 
CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT 
CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG 
TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT 
TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT 
AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG 
CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GACGCCTGTC GGTTTGTGAC TAAAATTGTG 
GGTAAAAACC CATTACTCCT GACAGAACTG AATCTGAGTG ATCGTAAACT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTACTGCAG GATAAACACT GTAGAGTCAA CACAATAATG 
CTGAATAATA ACAGGATTAC AGCAGAAGGT TGTGCTGCTT TAACTTCAGC ATTTATTTTA 
AACCCATCAC ACCTGATAGA GCTCAATCTG AGTGGGAATA AACTAGGAGA CTCAGGAGTA 
GAGAAGATCT GTCCTCTGCT GGAAAATACA CAATGCCGAT TAGAGAAACT GAGTCTTTCA 
GACTGCAGTA TCAGTGAAGA AGGTTATAAA GCTCTGGCTT CAGCTCTGAG ATCAAACCCT 
TCACACCTGA TAGAGCTGGA TCTCAGAGGA AATGATCCTG GACAATCAGG AGTGAAGAAG 
CTCACTGATT TACTGCACAG TAGAAGCTGT AAATTCAGAT TTCTGAAGAG TTCTGCTGCA 
CAGGAAGCAT GTGACTATCT TACTAAAGTT CTGGGTATAA GTCCATTATT ACTGACAGAA 
CTGGATCTGA GTGAAGATAA ATTAGGAGAT CTGGATGGAG AGAAACTCTC TGCTCTTCTG 
ATGGACTCTC ATAGCAAAGT GAAGAAAATC AAGCTGAATA ATTGTGAGCT GACAGAGAAA 
AGCTGTTCAC TTCTAGCTGC AGTTCTCTCC TCCAAAACCA TCCTGAAAGA GATGAACCTG 
AACAACAGTC GTCTGCTGGA CTCAGGAGTC AGAGAAATCT GTGAGGGACT GAAGAATCCT 
GTGTGTGAGC TGAAAATACT CAGTCTTTCA GACTGCCGTA TCAGTGAAGA AGGTTATAAA 
GCTCTGGCTT CAGCTCTGAG ATCAAACCCT TCACACCTGA TAGAGCTGGA TCTCAGAGGA 
AATGATCCTG GACAATCAGG AGTGAAGGAG CTCACTGACT TACTGGAGGA TCCACACTGT 
ACACTGAAGA CACTGAGGTT TTTGAGTCCT GCTGCTGATG AAGGCTGTCA CTTTGTGACT 
GAAATGGTGG GTAAAAACCC ATTACTCCTG AAAGAACTGA ATCTGAGTGA TCGTGTACTA 
GGAGACACAC GAGTGAATCA ACTCTCTGCT CTGCTGCAGG ATAAACACTG TCAGATCCAC 
ACACTCATTC TGTGTTATTG CAGTATTACA GAGGAACAGT GTGTGATCCT GACTTCAGCT 
CTGAAATCAA ACCCATCACA CCTGAGAGAA CTGAACCTCA GTGGAAACAA TCTAGGAGAC 
TCTGCAGTGA AAAAACTCAG TGATTTACTG ATGAACCAAC AATTCAAGCT GGAGAAACTA 
CGTCTGAGTG ATTGCAGTAT TACAGAGAAA CAGTGTGTGA TCCTGACTTC AGCTCTGAAA 
TCAAACCCAT CACACCTGAG AGAACTGAAC CTCAATGGGA ATAGACTAGG AGACTCTGGA 
GCTTATGGAC TTGGCCATCT GCTGAGCAGT GTGGATTGCA AGCTGAATAA ACTACATCTG 
TGTGATTGCA GTATTACAGG GAAACAGTTG CACTTCCTGA TTGCAGTTAT GTGTATGGAC 
CCATCACACC TGAGGGTACT GGACCTCAGT AAGAATCAAA TAAAAAGCAG AGGAATGAAC 
ATGCTGTGTG ATGTGCTGAA TAAACCACGC TGTAAACTCA AGAGTTTAAG ACTAAATGAC 
TGTGGCATTA CAGATGTTAC ACACTTAACT CAGTCTTTGG CTGAGAACAA AGCACTGGAG 
TTCTTAAAAG AGCTTGAACT GAGTAAAAAT CAAATGGGAG ACTCCGAAAA GCAAAAACTC 
AGTGACCTGC TGCGAGACTC AAACTGTAAA CTGAGGTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_017354579.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210068.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X9, mRNA.

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CloneID	ODa53642
Clone ID Related Accession (Same CDS sequence)	XM_017354579.1
Accession Version	XM_017354579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC568664 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG 
TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA 
GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT 
CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT 
CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG 
TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT 
TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT 
AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG 
CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAAGGCTGTC ACTTTGTGAC TGAAATGGTG 
GGTAAAAACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGTACT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTGCTGCAG GATAAACACT GTCAGATCCA CACACTCATT 
CTGTGTTATT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGAAACA ATCTAGGAGA CTCTGCAGTG 
AAAAAACTCA GTGATTTACT GATGAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT 
GATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTTATGGA 
CTTGGCCATC TGCTGAGCAG TGTGGATTGC AAGCTGAATA AACTACATCT GTGTGATTGC 
AGTATTACAG GGAAACAGTT GCACTTCCTG ATTGCAGTTA TGTGTATGGA CCCATCACAC 
CTGAGGGTAC TGGACCTCAG TAAGAATCAA ATAAAAAGCA GAGGAATGAA CATGCTGTGT 
GATGTGCTGA ATAAACCACG CTGTAAACTC AAGAGTTTAA GACTAAATGA CTGTGGCATT 
ACAGATGTTA CACACTTAAC TCAGTCTTTG GCTGAGAACA AAGCACTGGA GTTCTTAAAA 
GAGCTTGAAC TGAGTAAAAA TCAAATGGGA GACTCCGAAA AGCAAAAACT CAGTGACCTG 
CTGCGAGACT CAAACTGTAA ACTGAGGTTA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210068.1
CDS	182..6397
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLRLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLQQDPHCTLKILRFLSPAADEACQFLTGVVGKNLLLLKELNLSGHELGLSDSFQMLVLLLHDKHCTLNTLILSDCSITEEGYKALASALTSNPSYLIELDLRGNDPGQSGVKELTDLLLDERCSLKIIRFLKSPAAQESCDFLTEVLDVNPLLLTELDLSEDKLGDLDGEKISAFLMDPHSKVEKIKLNNCKLLEKSSSVLATVLSSKTILKELNLNNSRLLDSGVREICEGLKNPVCELKILKLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTELLQDPHCTLKTLRFLSPAADEGCQFVTGIVGKNPLLLKELNLSDRELGDTRVNQLSALLQDKHCKLNTLILSKCGVTVESCSALATVLRSETSLKELDMSNNNLQESGVKKLQSGLKNTNCTLEKLSLLDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKKLTELLKPDYTLKTLRFLSDDADEACTHLNKVLGGNLLLLRELNLSKLDLGPSGLEKLAAVLKDKHCKLNTLILNNSSMSEEDCRVLAEAFNSNPSNLTELNLSGTKLTNSGMKSFTTLFQNQQCRLEKLKFSSMCITAEGCAALASAFNSNPSHLIELNLSENKLGDSGVTEISSLLANSQCMLKILGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSPAADEGCHFVTEMVGKNPLLLKELNLSDRVLGDTRVNQLSALLQDKHCQIHTLILCYCSITEEQCVILTSALKSNPSHLRELNLSGNNLGDSAVKKLSDLLMNQQFKLEKLRLSDCSITEKQCVILTSALKSNPSHLRELNLNGNRLGDSGAYGLGHLLSSVDCKLNKLHLCDCSITGKQLHFLIAVMCMDPSHLRVLDLSKNQIKSRGMNMLCDVLNKPRCKLKSLRLNDCGITDVTHLTQSLAENKALEFLKELELSKNQMGDSEKQKLSDLLRDSNCKLRLD

Target ORF information:

RefSeq Version	XM_017354579.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354579.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GGCTTTCAGA CTGCAGTATC 
AGTGAAGAAG GTTATAAAGC TCTGGCTTCA GCTCTGAGAT CAAACCCTTC ACACCTGATA 
GAGCTGGATC TCAGAGGAAA TGATCCTGGA CAATCAGGAG TGAAGGAGCT CACTGATTTA 
CAGCAGGATC CACACTGTAC ACTGAAGATA CTGAGGTTTT TGAGTCCTGC TGCTGATGAA 
GCCTGTCAGT TTTTGACTGG AGTTGTGGGT AAAAACCTGT TACTCTTGAA AGAGCTGAAT 
CTGAGTGGAC ATGAACTAGG ACTCTCAGAC AGCTTTCAAA TGCTTGTTCT TTTACTGCAT 
GATAAACACT GTACGCTCAA CACACTGATT CTTTCAGATT GCAGTATCAC AGAAGAAGGT 
TATAAAGCTC TGGCTTCAGC TCTGACATCA AACCCATCAT ACCTGATAGA GCTGGATCTC 
AGAGGAAATG ATCCTGGACA ATCAGGAGTG AAGGAACTCA CTGATTTACT ACTGGATGAA 
CGCTGTAGCT TAAAAATCAT CAGGTTTCTG AAAAGTCCTG CTGCACAAGA GTCATGTGAT 
TTTCTCACTG AAGTTCTGGA TGTAAATCCA TTATTACTGA CAGAACTGGA TCTGAGTGAA 
GATAAATTAG GAGATCTGGA TGGAGAGAAA ATCTCTGCTT TTTTGATGGA CCCTCATAGC 
AAAGTGGAGA AAATCAAGCT GAATAATTGT AAGCTGCTTG AGAAAAGCTC ATCAGTTTTA 
GCAACAGTTC TGTCTTCCAA AACCATCCTG AAAGAGCTGA ACCTGAACAA CAGTCGTCTG 
CTGGACTCAG GAGTCAGAGA GATCTGTGAG GGACTGAAGA ATCCTGTGTG TGAGCTGAAG 
ATACTCAAGC TTTCAGACTG CAGTATCAGT GAAGAAGGTT ATAAAGCTCT GGCTTCAGCT 
CTGAGATCAA ACCCTTCACA CCTGATAGAG CTGGATCTCA GAGGAAATGA TCCTGGACAA 
TCAGGAGTGA AGGAGCTCAC TGAGTTACTG CAGGATCCAC ACTGTACACT GAAGACACTG 
AGGTTTTTGA GTCCTGCTGC TGATGAAGGC TGTCAGTTTG TGACTGGAAT TGTGGGTAAA 
AACCCATTAC TTCTGAAAGA GCTAAATCTG AGTGATCGTG AACTAGGAGA CACACGAGTG 
AATCAACTCT CTGCTCTACT GCAGGATAAA CACTGTAAAC TCAACACACT CATTCTTAGT 
AAATGTGGTG TAACAGTGGA AAGCTGTTCA GCTCTGGCTA CAGTCCTGAG ATCAGAGACC 
AGTCTAAAAG AGCTTGACAT GAGCAACAAT AATCTGCAGG AATCAGGAGT GAAGAAACTC 
CAGAGCGGAT TAAAAAACAC AAACTGCACA CTGGAAAAAC TCAGTCTTTT AGACTGCAGT 
ATCAGTGAAG AAGGTTATAA AGCTCTGGCT TCAGCTCTGA GATCAAACCC TTCACACCTG 
ATAGAGCTGG ATCTCAGAGG AAATGATCCT GGACAATCAG GAGTGAAGAA GCTCACTGAG 
TTACTGAAAC CAGACTATAC ACTGAAGACA CTGAGGTTTT TGAGTGATGA TGCAGATGAA 
GCCTGTACAC ATCTGAATAA AGTTCTGGGT GGAAACCTGT TACTCTTGAG AGAGCTGAAT 
CTGAGTAAAC TTGATCTTGG ACCATCAGGA TTGGAGAAAC TTGCTGCTGT ACTGAAAGAT 
AAACACTGTA AACTCAACAC ACTGATTCTG AATAACAGCA GTATGTCAGA AGAAGATTGT 
CGTGTTTTGG CTGAAGCTTT TAATTCAAAC CCATCAAATC TGACAGAGCT GAATTTGAGT 
GGGACTAAAC TCACAAACTC AGGAATGAAG AGCTTCACCA CTCTGTTTCA AAACCAACAG 
TGTAGACTGG AAAAGCTGAA GTTCAGCAGC ATGTGTATTA CAGCAGAAGG TTGTGCTGCT 
TTGGCTTCAG CATTTAACTC AAATCCTTCA CACCTGATAG AGCTCAATCT GAGTGAGAAT 
AAACTAGGAG ACTCAGGAGT AACAGAAATC TCCAGTCTAC TGGCAAACTC GCAGTGCATG 
CTGAAGATAC TCGGTCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCACTGAT TTACTGCAGG ATCCACACTG TACACTGAAG 
ACACTGAGGT TTTTGAGTCC TGCTGCTGAT GAAGGCTGTC ACTTTGTGAC TGAAATGGTG 
GGTAAAAACC CATTACTCCT GAAAGAACTG AATCTGAGTG ATCGTGTACT AGGAGACACA 
CGAGTGAATC AACTCTCTGC TCTGCTGCAG GATAAACACT GTCAGATCCA CACACTCATT 
CTGTGTTATT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGACTTCAGC TCTGAAATCA 
AACCCATCAC ACCTGAGAGA ACTGAACCTC AGTGGAAACA ATCTAGGAGA CTCTGCAGTG 
AAAAAACTCA GTGATTTACT GATGAACCAA CAATTCAAGC TGGAGAAACT ACGTCTGAGT 
GATTGCAGTA TTACAGAGAA ACAGTGTGTG ATCCTGACTT CAGCTCTGAA ATCAAACCCA 
TCACACCTGA GAGAACTGAA CCTCAATGGG AATAGACTAG GAGACTCTGG AGCTTATGGA 
CTTGGCCATC TGCTGAGCAG TGTGGATTGC AAGCTGAATA AACTACATCT GTGTGATTGC 
AGTATTACAG GGAAACAGTT GCACTTCCTG ATTGCAGTTA TGTGTATGGA CCCATCACAC 
CTGAGGGTAC TGGACCTCAG TAAGAATCAA ATAAAAAGCA GAGGAATGAA CATGCTGTGT 
GATGTGCTGA ATAAACCACG CTGTAAACTC AAGAGTTTAA GACTAAATGA CTGTGGCATT 
ACAGATGTTA CACACTTAAC TCAGTCTTTG GCTGAGAACA AAGCACTGGA GTTCTTAAAA 
GAGCTTGAAC TGAGTAAAAA TCAAATGGGA GACTCCGAAA AGCAAAAACT CAGTGACCTG 
CTGCGAGACT CAAACTGTAA ACTGAGGTTA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC568664 ( XM_017354580.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210069.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X22, mRNA.

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CloneID	ODa53643
Clone ID Related Accession (Same CDS sequence)	XM_017354580.1
Accession Version	XM_017354580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 12 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008805582.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GTTTAAGCAG CTGTTCTCTT 
ACAGATGAAA GTTATTCTGC TCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGGACC TCAGTCAGAA TAAACTGGGA GACTCTGCAG TGAAAAACCT CAGTGATTTA 
CTGATGAAGC CTCAATTTAA GCTGGAGAAA CTACATCTGT ATGAGTGCAG TATTACAGAG 
AAACAGTGTG AGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAGTT GGAGTCAAAT AAGAAACACA GGACTGAATC TCTTCTGTGA TGTGCTGAAG 
GATTCACGCT GTAAACTGGA GACACTGAGA TTGTTTGGTT GTGGCATCTC AGACGTTTCC 
TCTTTAACTC AGTGTTTGAT GAACACAAAA GCCCTGCAGT TTCTAAAAGA GCTTGATCTG 
AGATTCAATC ACATTGGAGA CTCAAAGCAA AAGCTCATTG AAGGGATACG AGACTCAAAC 
TGTAAACTGA GCTTCAGAAT AGAGGATCAG TTGCCGAAGC CTGATGACTC GAGATGTTGT 
ATATCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210069.1
CDS	182..3850
Translation	MIFVFLRLRSENSLDCFIVAGLISSSGLRERSCYILKLLHIISTLNFDMCFEVPPVVTFYSLFCVISVRSGSYVSSSVSVKSDDSKKGGQPNFSRETLSPTTSVQNESSDTDIQTQKKHMSFKDNLQGIFQDLENQILKFLKNELEKFKKILHEENTEYFVKDFGENRCRIKEAALDLTLYFLKEMKQDEAADTLEDELFLIHQLKWDLQKKYQRVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTEHEVRQIEVVSRRHEAQEIQVECRRLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFFPETKGLNLTRRNQFKVLFILDGLDECRLPLNFAGNETCLDVASPASLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEIRGFNDEQKEEYFRKRFTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNILEEKRNTVVKNNQADDAFKTLQESNTEDTPKTLTQMYTHFLHFQIQQSRRKYDGEYTPDVSWDKDAILSLGKLAFDNLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKEETGIVLGTMYCFVHLSVQEFIAALYAHLLLDINKKSVFDQDSKNRNESMIDLLKTAVDKTLESDKGHLDLFLRFLLGLSLQSNQRLLRGLLTQQDHNNQIKEEIVQYIKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTHLSKGSLSSVDLSPAQWSALAFVLLTSEEKMEEFELQKFKKSDECLIRLSAVIKTTRKALLNDCNLTDRSCSALPAVLGEENNLKELNMSNNNLQDSGVKLLCTGLKDIKCELEILRLSDCSITEEGYKALALALRSNPSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSTAAEEACQFVPGVVGKNPLLLKELNLSDCKLGDRRVNQLSALLQDKHCQINTLFLYNCSITEEQCLILSSALKSNPSHLRKLNLSENQIRNTGVNLLCDVLKDSHCKLKTLSLSSCSLTDESYSALTSALKSNPSHLRELDLSQNKLGDSAVKNLSDLLMKPQFKLEKLHLYECSITEKQCEILTSALKSNPSHLRELNLSWSQIRNTGLNLFCDVLKDSRCKLETLRLFGCGISDVSSLTQCLMNTKALQFLKELDLRFNHIGDSKQKLIEGIRDSNCKLSFRIEDQLPKPDDSRCCIS

Target ORF information:

RefSeq Version	XM_017354580.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12 (LOC568664), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354580.1

ORF Insert Sequence:

ATGATATTTG TTTTTCTAAG ATTGAGAAGT GAAAATTCAC TTGACTGTTT CATTGTAGCA 
GGTCTGATCA GTTCATCGGG ACTTAGAGAG AGATCTTGCT ATATTCTTAA ATTGTTACAC 
ATCATTTCCA CACTAAATTT CGACATGTGC TTTGAAGTTC CTCCTGTAGT CACTTTTTAC 
TCTCTTTTTT GTGTCATCAG TGTAAGATCA GGTTCGTATG TATCCAGCTC TGTGTCTGTG 
AAGAGTGATG ATTCAAAAAA AGGAGGTCAG CCAAACTTCA GTAGAGAAAC ACTGTCACCT 
ACCACAAGTG TTCAAAATGA GTCTTCAGAC ACAGACATCC AGACTCAGAA AAAACACATG 
AGTTTTAAAG ACAATCTTCA GGGGATCTTC CAGGATCTTG AGAACCAAAT ATTAAAATTT 
CTGAAGAATG AGCTAGAAAA GTTTAAGAAA ATATTACATG AAGAAAATAC AGAATACTTT 
GTAAAGGACT TTGGTGAGAA CAGATGCAGA ATAAAAGAAG CAGCTCTTGA TCTCACACTC 
TACTTCCTGA AAGAAATGAA GCAAGATGAA GCAGCAGATA CTCTAGAAGA TGAGCTGTTT 
CTCATTCATC AGCTGAAATG GGATCTACAA AAGAAGTATC AGCGTGTGTT TGAAGGAATT 
GCACAGCAAG GAGACTCCAC ACTCCTGAAG AACATCTACA CAGATCTTTA TATCACTCAG 
GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGTTTCTAGA 
CGACATGAAG CTCAAGAGAT ACAGGTTGAA TGCAGACGTT TGTTTGAAGC GTCTGAACAA 
GACAAGCAGA TCAGAACTGT CCTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT 
GTGCAAAAGT TTGTTCTGGA TTGGGCCGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA 
TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG 
CTTATAACTC AGTTTTTCCC AGAGACGAAA GGACTGAACC TTACAAGAAG GAATCAGTTC 
AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT 
AATGAGACGT GTCTAGATGT TGCATCACCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC 
ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC 
AGTAAGATTC CTCCTGACTG TATCGACAGA GTGACAGAGA TACGAGGATT CAATGATGAG 
CAAAAGGAGG AGTACTTCAG AAAAAGATTC ACAGATGAGA ATCAGGCCAA AGAAATCATT 
GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGTTGG 
ATTTCAGCCA CTGTTCTCCA GAACATTTTA GAGGAGAAGA GAAATACCGT TGTGAAAAAC 
AATCAGGCTG ATGACGCCTT CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG 
ACTCTGACAC AGATGTACAC ACACTTTCTG CACTTTCAGA TCCAGCAGAG CAGACGAAAG 
TATGATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG 
AAACTGGCAT TTGATAATCT GCTAAACAGC AACCTGATCT TCTATGACAC CGACCTGGAA 
GCCTGTGGTA TTGATGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT 
AAGGAGGAAA CAGGGATTGT TCTTGGCACC ATGTACTGCT TTGTTCACTT GAGCGTTCAA 
GAGTTTATTG CAGCCCTTTA TGCACATCTG TTACTAGACA TCAACAAGAA AAGTGTGTTT 
GATCAAGACT CTAAAAACAG AAATGAGTCC ATGATTGATT TGCTAAAGAC TGCAGTGGAC 
AAGACACTAG AGAGTGACAA AGGACACCTG GACCTTTTCC TTCGCTTCCT TCTCGGTCTG 
TCTCTCCAGT CCAATCAACG GCTCTTACGA GGTCTGTTGA CACAGCAAGA CCACAATAAC 
CAGATCAAAG AGGAAATAGT TCAGTACATC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG TTGATCTTTC ACCTGCCCAG 
TGGTCTGCTC TGGCCTTTGT GTTGTTGACA TCAGAGGAGA AGATGGAGGA GTTTGAGCTT 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTTAT CAAAACTACC 
AGAAAAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TCTGCCTGCA 
GTTCTCGGAG AAGAAAACAA TCTGAAAGAG CTGAACATGA GCAATAACAA TCTGCAGGAT 
TCAGGAGTGA AGCTGCTCTG CACTGGACTG AAAGATATTA AGTGCGAGTT AGAAATACTA 
AGACTTTCAG ACTGCAGTAT TACAGAAGAA GGTTATAAAG CTCTGGCTTT AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACAATCAGGA 
GTGAAGGAGC TCACTGATTT ACTGCAGGAT CCACACTGTA CACTGAAGAC ACTAAGGTTT 
TTGAGTACTG CTGCAGAGGA AGCCTGTCAG TTTGTGCCTG GAGTTGTGGG TAAAAACCCG 
TTACTCCTTA AAGAACTGAA TCTGAGTGAT TGTAAACTAG GAGACAGGCG AGTGAACCAA 
CTCTCTGCTT TACTGCAGGA TAAACACTGT CAGATCAACA CACTCTTTCT GTATAATTGC 
AGTATTACAG AGGAACAGTG TTTGATCCTG TCTTCAGCTC TGAAATCAAA CCCATCACAC 
TTGAGAAAAC TGAACCTCAG TGAGAATCAA ATAAGAAACA CAGGAGTGAA TCTCTTATGT 
GATGTACTGA AGGATTCACA CTGTAAACTG AAGACACTGA GTTTAAGCAG CTGTTCTCTT 
ACAGATGAAA GTTATTCTGC TCTGACTTCA GCTCTGAAAT CAAACCCATC ACACCTGAGA 
GAACTGGACC TCAGTCAGAA TAAACTGGGA GACTCTGCAG TGAAAAACCT CAGTGATTTA 
CTGATGAAGC CTCAATTTAA GCTGGAGAAA CTACATCTGT ATGAGTGCAG TATTACAGAG 
AAACAGTGTG AGATCCTGAC TTCAGCTCTG AAATCAAACC CATCACACCT GAGAGAACTG 
AACCTCAGTT GGAGTCAAAT AAGAAACACA GGACTGAATC TCTTCTGTGA TGTGCTGAAG 
GATTCACGCT GTAAACTGGA GACACTGAGA TTGTTTGGTT GTGGCATCTC AGACGTTTCC 
TCTTTAACTC AGTGTTTGAT GAACACAAAA GCCCTGCAGT TTCTAAAAGA GCTTGATCTG 
AGATTCAATC ACATTGGAGA CTCAAAGCAA AAGCTCATTG AAGGGATACG AGACTCAAAC 
TGTAAACTGA GCTTCAGAAT AGAGGATCAG TTGCCGAAGC CTGATGACTC GAGATGTTGT 
ATATCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC568664 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

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