slc9a3.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc9a3.2
Entrez Gene ID	568314
Full Name	solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2
Synonyms	nhe3b,si:dkey-3n22.6
General protein information	Preferred Names solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 Names solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 sodium hydrogen exchanger 3b solute carrier family 9 (sodium/hydrogen exchanger), member 3, tandem duplicate 2 solute carrier family 9 (sodium/hydrogen exchanger), member 3.2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113479.1	NP_001106951.1	solute carrier family 9 member A3, tandem duplicate 2 precursor
XM_021468124.1	XP_021323799.1	solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 isoform X1
XM_009294056.2	XP_009292331.1	solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 isoform X1
XM_009294056.3	XP_009292331.1	solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Slc9a3	NP_036786.1
Gallus gallus (chicken)	SLC9A3	XP_004935132.1
Pan troglodytes (chimpanzee)	SLC9A3	XP_003954578.1
Homo sapiens (human)	SLC9A3	NP_004165.2
Danio rerio (zebrafish)	slc9a3.2	NP_001106951.1
Danio rerio (zebrafish)	slc9a3.1	NP_001106944.1
Xenopus tropicalis (tropical clawed frog)	LOC100492870	XP_004915375.1
Bos taurus (cattle)	SLC9A3	NP_001179083.1
Mus musculus (house mouse)	Slc9a3	NP_001074529.1

Gene Ontology
Bibliography

Related articles in PubMed

A role for sodium-chloride cotransporters in the rapid regulation of ion uptake following acute environmental acidosis: new insights from the zebrafish model.
Kwong RW, Perry SF
American journal of physiology. Cell physiology311(6)C931-C941(2016 Dec)

Oestrogen-related receptor ? is required for transepithelial H+ secretion in zebrafish.
Guh YJ, Yang CY, Liu ST, Huang CJ, Hwang PP
Proceedings. Biological sciences283(1825)20152582(2016 Feb)

The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models.
Shu Y, Lou Q, Dai Z, Dai X, He J, Hu W, Yin Z
Scientific reports618597(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rhcg1 and NHE3b are involved in ammonium-dependent sodium uptake by zebrafish larvae acclimated to low-sodium water.
Title: Rhcg1 and NHE3b are involved in ammonium-dependent sodium uptake by zebrafish larvae acclimated to low-sodium water.

expressed in gills and colocalized with V-H(+)-ATPase but not with Na(+)-K(+)-ATPase
Title: Gene expression of Na+/H+ exchanger in zebrafish H+ -ATPase-rich cells during acclimation to low-Na+ and acidic environments.

The following slc9a3.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc9a3.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03417	NM_001113479.1 Latest version!	Danio rerio solute carrier family 9 member A3, tandem duplicate 2 (slc9a3.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa45399	XM_021468124.1 Latest version!	Danio rerio solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 (slc9a3.2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa45399	XM_009294056.3 Latest version!	Danio rerio solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 (slc9a3.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa45399	XM_009294056.2	Danio rerio solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 (slc9a3.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc9a3.2 ( NM_001113479.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001106951.1 Danio rerio solute carrier family 9 member A3, tandem duplicate 2 (slc9a3.2), mRNA.

Price & Availability↑

CloneID	ODa03417
Clone ID Related Accession (Same CDS sequence)	NM_001113479.1
Accession Version	NM_001113479.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-22
Organism	Danio rerio(zebrafish)
Product	solute carrier family 9 member A3, tandem duplicate 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF591980.1. On Jan 16, 2008 this sequence version replaced XM_691636.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF591980.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTTTT CTACTCTTCT TCTAGCATTT TTGGTGGTCT CCGGTGCATT ACATGAGGCT 
GCTGCGGGGT TAGATTTCTA TGGTGCTGAA GGAAAGCAGA ATTACTCTTC AAGATCTTCA 
GCTGAAGGTT CTGCTTCTTC AGGCAACAGC AGTCATTCTG CCACTATCAC CACATTACCC 
ATCGTCACCT GGAAATGGCA TCATGTAGAG ACTCCATACC TGGTGGCCCT GTGGATTCTG 
ACCTGCTGGA TCTGTAAATT AGTCACTGAG CTGAACCACA ACATCACCAG TGTGATTCCG 
GAAAGTGGTC TTCTCATCAT CCTGGGCTTT ATTTTGGGAG GAATCGTCTG GGGAGCAGAC 
AAAGCGCAGA CTTTTAAACT CATCCCTGTT AACTTCTTCT TTTACCTGCT GCCCCAGATC 
ATCCTGGATG CCAGTTACTG CATGCCAAAC AAACTGTTCT TCGGCAACCT GGGAGCCATT 
CTTGTCCATG CCATTATTGG GACGTGCTGG AATGCGGGCA CAGTTGGCAT TGCTTTATGG 
GCTTGCTATG AAGGGGGCGC AATGGGGACC TTGAACATCG GCTGTCTGCA GTTCCTGCTG 
TTTGGAGCCC TGATGTCTGC TGTGGACCCT GTGGCTGTGA TCGCTGTGTT TGAAGAAGTG 
CATGTGAATG AAGTGCTTTA CATCCTGGTG TTTGGAGAGT CACTGCTCAA TGATGGAGTC 
ACAGTGGTGC TTTATAATGT GTTTGATGCA TTTGTGTCTC TGGGAGGCCC AAAGATCAAT 
GCTGCGGAGA TCATTAAAGG AATCGTTTCA TTCGCTGTGG TGGCTTTTGG AGGTTCATTT 
CTCGGTGTTG TGTTTGCCGT CCTGACATCC ATGCTGACTC GGGTCACCAA AAACGTCCAG 
ATCATTGAAG CCGGTTTCAT CATCGTGCTG GGCTATCTGT CTTACCTGAC GGCTGAGATG 
CTCTCGCTTT CTGCCATCCT TTCACTGACT TTCTGCGGTG TGTGCTGTCA GAAATACATC 
AATGCCAACA TGGATGAGAA ATCCGTCACA TGCCTGCGTT ACTCTCTGAA AGTGTTGGCC 
AACGGCTCCG AGACCATGAT CTTCGTTTTC TTGGGAGTTT CGGCCATTGA TCAAACCATC 
TGGGTTTGGA ACACAGGATT CATCCTCCTC ACCCTCCTCT TCATCGTTGT ATTCAGGATG 
ATGGGGGTTT TCTTCCTCAA CTGGATCCTC AATCAGTCTA GACTGATCCC TATAGACCTC 
ACTGATCAGT TGGTAATGGG CTACGGTGGG CTGCGAGGAG CAGTGGCGTA TGGACTCGCA 
GCCTCTTTAG ATGAGAACAA GATCCCGGAG AAAAATCTAA TGCTTGGCAC TACACTCATT 
GTAGTGTATT TCACTGTCAT TCTGCAGGGT ATCACAATGA AGCCATTGGT TAATTGGCTA 
AAAGTTAAAA AGGCAACGCA GTCCGATCTG ACCCTCAACG GAAAACTGAA CAACAGGGTT 
TTTGAACACA CATTGACAGG AATGGAGGAC ATCTGTGGCC GAATGGGAGA TAACTGGTGG 
ACGAGACATT GGAACCATTT TGAAGAAAAA TATGTCTGCT GGCTTCTAAT GACGAGTGAG 
GCCAGAAAGC GCAATGACAA AATCTTGGAT GCCTTCCACA AACTCAATGT TGAGGACGCC 
ACCAAATATG TCAATGAGGG TGAAAGCAAA GGTTCTCTGG CGTTCATTCG TAGTTACGAC 
AGTGCCTCTG TAGACTTTAA GAAGAAGCTT GCTTTGGAGT ACGCAGACAT CATTCCCGAT 
ATCATGGCTG ACATGTCCGA ATATGACTTT GACATTGACA GTGTTCCCGT GACCTCTGTC 
ATGAAGAACC CGATTCCATC TGTATCTCTG GATATCCATG AGCAGGAGCG GAGGGGCATG 
TCAAACGACC TCAACGCCCA TCATCTACTA GAGCAGCACT TGTACAAGAG CAGGAGAAAT 
AACCGTGCCA CTTACAGCCG CAGTCACTAC AACACCTCAG AGAATCCAGA CGAAATGCAG 
GAGATCTTCC AGAGAACCAT GGGGAACCGT CTGGAGTCCT TTAAGTCAGC TAAGATGGGA 
GTCAACCCTG CCAAAAAAGT CTCTAAACAT CCCAAGAAGG ATGCACCACA TAAGCCTAAT 
GGAAAGCCAG CAGACCCAAA CAGAGACCAT CTTTATGGCG ATGAAGACTT TGAGTTTTCT 
GCAGACAGCG CCTCTAGCAG TGAAAATACC TGTCAGTTCC CCAGAAGAGT CATAAACACG 
ACTGGAGCTG GTGTGGATAA TCCAGCATAT ATCCCTGAAC TGGACACCTC TCCATCCATG 
CGAAACCCAC CCTGGCAAAC AGAGACAGGC CACAACACAG CTGTAGCACC TTCTCAGAGA 
GCTCAGGGCC GACTGCCCTG GACGCCCACT AACCTGAGAC GTCTGGCGCC GCTGAGAGTG 
AGCAGTCGCT CCACAGACTC CTTCGCCATG CCTGAACCTG CAGCTGACGA GGAGACGCCT 
GAGGAAAAAC CTGCAACACA TCATACTAGA CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106951.1
CDS	100..2655
Translation	MAFSTLLLAFLVVSGALHEAAAGLDFYGAEGKQNYSSRSSAEGSASSGNSSHSATITTLPIVTWKWHHVETPYLVALWILTCWICKLVTELNHNITSVIPESGLLIILGFILGGIVWGADKAQTFKLIPVNFFFYLLPQIILDASYCMPNKLFFGNLGAILVHAIIGTCWNAGTVGIALWACYEGGAMGTLNIGCLQFLLFGALMSAVDPVAVIAVFEEVHVNEVLYILVFGESLLNDGVTVVLYNVFDAFVSLGGPKINAAEIIKGIVSFAVVAFGGSFLGVVFAVLTSMLTRVTKNVQIIEAGFIIVLGYLSYLTAEMLSLSAILSLTFCGVCCQKYINANMDEKSVTCLRYSLKVLANGSETMIFVFLGVSAIDQTIWVWNTGFILLTLLFIVVFRMMGVFFLNWILNQSRLIPIDLTDQLVMGYGGLRGAVAYGLAASLDENKIPEKNLMLGTTLIVVYFTVILQGITMKPLVNWLKVKKATQSDLTLNGKLNNRVFEHTLTGMEDICGRMGDNWWTRHWNHFEEKYVCWLLMTSEARKRNDKILDAFHKLNVEDATKYVNEGESKGSLAFIRSYDSASVDFKKKLALEYADIIPDIMADMSEYDFDIDSVPVTSVMKNPIPSVSLDIHEQERRGMSNDLNAHHLLEQHLYKSRRNNRATYSRSHYNTSENPDEMQEIFQRTMGNRLESFKSAKMGVNPAKKVSKHPKKDAPHKPNGKPADPNRDHLYGDEDFEFSADSASSSENTCQFPRRVINTTGAGVDNPAYIPELDTSPSMRNPPWQTETGHNTAVAPSQRAQGRLPWTPTNLRRLAPLRVSSRSTDSFAMPEPAADEETPEEKPATHHTRL

Target ORF information:

RefSeq Version	NM_001113479.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 9 member A3, tandem duplicate 2 (slc9a3.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113479.1

ORF Insert Sequence:

ATGGCGTTTT CTACTCTTCT TCTAGCATTT TTGGTGGTCT CCGGTGCATT ACATGAGGCT 
GCTGCGGGGT TAGATTTCTA TGGTGCTGAA GGAAAGCAGA ATTACTCTTC AAGATCTTCA 
GCTGAAGGTT CTGCTTCTTC AGGCAACAGC AGTCATTCTG CCACTATCAC CACATTACCC 
ATCGTCACCT GGAAATGGCA TCATGTAGAG ACTCCATACC TGGTGGCCCT GTGGATTCTG 
ACCTGCTGGA TCTGTAAATT AGTCACTGAG CTGAACCACA ACATCACCAG TGTGATTCCG 
GAAAGTGGTC TTCTCATCAT CCTGGGCTTT ATTTTGGGAG GAATCGTCTG GGGAGCAGAC 
AAAGCGCAGA CTTTTAAACT CATCCCTGTT AACTTCTTCT TTTACCTGCT GCCCCAGATC 
ATCCTGGATG CCAGTTACTG CATGCCAAAC AAACTGTTCT TCGGCAACCT GGGAGCCATT 
CTTGTCCATG CCATTATTGG GACGTGCTGG AATGCGGGCA CAGTTGGCAT TGCTTTATGG 
GCTTGCTATG AAGGGGGCGC AATGGGGACC TTGAACATCG GCTGTCTGCA GTTCCTGCTG 
TTTGGAGCCC TGATGTCTGC TGTGGACCCT GTGGCTGTGA TCGCTGTGTT TGAAGAAGTG 
CATGTGAATG AAGTGCTTTA CATCCTGGTG TTTGGAGAGT CACTGCTCAA TGATGGAGTC 
ACAGTGGTGC TTTATAATGT GTTTGATGCA TTTGTGTCTC TGGGAGGCCC AAAGATCAAT 
GCTGCGGAGA TCATTAAAGG AATCGTTTCA TTCGCTGTGG TGGCTTTTGG AGGTTCATTT 
CTCGGTGTTG TGTTTGCCGT CCTGACATCC ATGCTGACTC GGGTCACCAA AAACGTCCAG 
ATCATTGAAG CCGGTTTCAT CATCGTGCTG GGCTATCTGT CTTACCTGAC GGCTGAGATG 
CTCTCGCTTT CTGCCATCCT TTCACTGACT TTCTGCGGTG TGTGCTGTCA GAAATACATC 
AATGCCAACA TGGATGAGAA ATCCGTCACA TGCCTGCGTT ACTCTCTGAA AGTGTTGGCC 
AACGGCTCCG AGACCATGAT CTTCGTTTTC TTGGGAGTTT CGGCCATTGA TCAAACCATC 
TGGGTTTGGA ACACAGGATT CATCCTCCTC ACCCTCCTCT TCATCGTTGT ATTCAGGATG 
ATGGGGGTTT TCTTCCTCAA CTGGATCCTC AATCAGTCTA GACTGATCCC TATAGACCTC 
ACTGATCAGT TGGTAATGGG CTACGGTGGG CTGCGAGGAG CAGTGGCGTA TGGACTCGCA 
GCCTCTTTAG ATGAGAACAA GATCCCGGAG AAAAATCTAA TGCTTGGCAC TACACTCATT 
GTAGTGTATT TCACTGTCAT TCTGCAGGGT ATCACAATGA AGCCATTGGT TAATTGGCTA 
AAAGTTAAAA AGGCAACGCA GTCCGATCTG ACCCTCAACG GAAAACTGAA CAACAGGGTT 
TTTGAACACA CATTGACAGG AATGGAGGAC ATCTGTGGCC GAATGGGAGA TAACTGGTGG 
ACGAGACATT GGAACCATTT TGAAGAAAAA TATGTCTGCT GGCTTCTAAT GACGAGTGAG 
GCCAGAAAGC GCAATGACAA AATCTTGGAT GCCTTCCACA AACTCAATGT TGAGGACGCC 
ACCAAATATG TCAATGAGGG TGAAAGCAAA GGTTCTCTGG CGTTCATTCG TAGTTACGAC 
AGTGCCTCTG TAGACTTTAA GAAGAAGCTT GCTTTGGAGT ACGCAGACAT CATTCCCGAT 
ATCATGGCTG ACATGTCCGA ATATGACTTT GACATTGACA GTGTTCCCGT GACCTCTGTC 
ATGAAGAACC CGATTCCATC TGTATCTCTG GATATCCATG AGCAGGAGCG GAGGGGCATG 
TCAAACGACC TCAACGCCCA TCATCTACTA GAGCAGCACT TGTACAAGAG CAGGAGAAAT 
AACCGTGCCA CTTACAGCCG CAGTCACTAC AACACCTCAG AGAATCCAGA CGAAATGCAG 
GAGATCTTCC AGAGAACCAT GGGGAACCGT CTGGAGTCCT TTAAGTCAGC TAAGATGGGA 
GTCAACCCTG CCAAAAAAGT CTCTAAACAT CCCAAGAAGG ATGCACCACA TAAGCCTAAT 
GGAAAGCCAG CAGACCCAAA CAGAGACCAT CTTTATGGCG ATGAAGACTT TGAGTTTTCT 
GCAGACAGCG CCTCTAGCAG TGAAAATACC TGTCAGTTCC CCAGAAGAGT CATAAACACG 
ACTGGAGCTG GTGTGGATAA TCCAGCATAT ATCCCTGAAC TGGACACCTC TCCATCCATG 
CGAAACCCAC CCTGGCAAAC AGAGACAGGC CACAACACAG CTGTAGCACC TTCTCAGAGA 
GCTCAGGGCC GACTGCCCTG GACGCCCACT AACCTGAGAC GTCTGGCGCC GCTGAGAGTG 
AGCAGTCGCT CCACAGACTC CTTCGCCATG CCTGAACCTG CAGCTGACGA GGAGACGCCT 
GAGGAAAAAC CTGCAACACA TCATACTAGA CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc9a3.2 ( XM_021468124.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323799.1 Danio rerio solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 (slc9a3.2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa45399
Clone ID Related Accession (Same CDS sequence)	XM_021468124.1 , XM_009294056.2 , XM_009294056.3
Accession Version	XM_021468124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCAT TTTTGGTGGT CTCCGGTGCA TTACATGAGG CTGCTGCGGG GTTAGATTTC 
TATGGTGCTG AAGGAAAGCA GAATTACTCT TCAAGATCTT CAGCTGAAGG TTCTGCTTCT 
TCAGGCAACA GCAGTCATTC TGCCACTATC ACCACATTAC CCATCGTCAC CTGGAAATGG 
CATCATGTAG AGACTCCATA CCTGGTGGCC CTGTGGATTC TGACCTGCTG GCTCTGTAAA 
TTAGTCACTG AGCTGAACCA CAACATCACC AGTGTGATTC CGGAAAGTGG TCTTCTCATC 
ATCCTGGGCT TTATTTTGGG AGGAATCGTC TGGGGAGCAG ACAAAGCGCA GACTTTTAAA 
CTCATCCCTG TTAACTTCTT CTATTACCTG CTGCCCCAGA TCATCCTGGA TGCCAGTTAC 
TGCATGCCAA ACAAACTATT CTTCGGCAAC CTGGGAGCCA TTCTTGTCCA TGCCATTATT 
GGGACGTGTT GGAATGCGGG CACAGTTGGC ATTGCTTTAT GGGCTTGCTA TGAAGGGGGC 
GCAATGGGGA CCTTGAACAT CGGCTGTCTG CAGTTCCTGC TGTTTGGAGC CCTGATGTCT 
GCTGTGGACC CTGTGGCTGT GATCGCTGTG TTTGAAGAAG TGCATGTGAA TGAAGTGCTT 
TACATCCTGG TGTTTGGAGA GTCACTGCTC AATGATGGAG TCACAGTGGT GCTTTATAAT 
GTGTTTGATG CATTTGTGTC TCTGGGAGGC CCAAAGATCA ATGCTGCAGA GATCATTAAA 
GGAATCGTTT CATTCGCTGT GGTGGCTTTT GGAGGTTCAT TTCTCGGTGT TGTGTTTGCC 
GTCCTGACAT CCATGCTGAC TCGGGTCACC AAAAACGTCC AGATCATTGA AGCCGGTTTC 
ATCATCGTGC TGGGCTATCT GTCTTACCTG ACGGCTGAGA TGCTCTCACT TTCTGCCATC 
CTTTCACTGA CTTTCTGCGG TGTGTGCTGT CAGAAATACA TCAATGCCAA CATGGATGAG 
AAATCCGTCA CATGCCTGCG TTACTCTCTA AAAGTGTTGG CCAACGGCTC CGAGACCATG 
ATCTTCGTTT TCTTGGGAGT TTCGGCCATT GATCAAACCA TCTGGGTTTG GAACACAGGA 
TTCATCCTCC TCACCCTCCT CTTCATCGTT GTATTCAGGA TGATGGGTGT TTTCTTCCTC 
AACTGGATCC TCAATCAGTC TAGACTGATC CCTATAGACC TCACTGATCA GTTGGTAATG 
GGCTACGGTG GGCTGCGAGG AGCAGTGGCG TACGGACTCG CAGCCTCTTT AGATGAGAAC 
AAGATCCCGG AGAAGAATCT AATGCTTGGC ACTACACTCA TTGTAGTGTA TTTCACTGTC 
ATTCTGCAGG GTATCACAAT GAAGCCATTG GTTAATTGGC TAAAAGTTAA AAAGGCAACG 
CAGTCCGATC TGACACTTAA CGGAAAACTG AACAACAGGG TTTTTGAACA CACATTGACA 
GGAATGGAGG ACATCTGTGG CCGAATGGGA GATAACTGGT GGACGAGACA TTGGAACCAT 
TTTGAAGAGA AATATGTCTG CTGGCTTCTA ATGACGAGTG AGGCCAGAAA GCGCAATGAC 
AAAATCTTGG ATGCCTTCCA CAAACTAAAT GTTGAGGACG CCACCAAATA TGTCAATGAG 
GGTGAAAGCA AAGGTTCTCT GGCGTTCATT CGTAGTTACG ACAGTGCCTC TGTAGACTTT 
AAGAAGAAGC TTGCTTTGGA GTACGCAGAC ATCATTCCCG ATATCATGGC TGACATGTCC 
GAATATGACT TTGACATTGA CAGTGTTCCC GTGACCTCTG TCATGAAGAA CCCGATTCCA 
TCTGTATCTC TGGATATCCA TGAGCAGGAG CGGAGGGGCA TGTCAAACGA CCTCAACGCC 
CATCATCTAC TAGAGCAGCA CTTGTACAAG AGCAGGAGAA ATAACCGTGC CACTTACAGC 
CGCAGTCACT ACAACACCTC AGAGAATCCA GACGAAATGC AGGAGATCTT CCAGAGAACC 
ATGAGGAACC GTCTGGAGTC CTTTAAGTCA GCTAAGATGG GAGTCAACCC TGCCAAAAAA 
GTCTCTAAAC ATCCCAAGAA GGATGCACCA CATAAGCCTA ATGGAAAGCC AGCAGACCCA 
AACAGAGACC ATCTTTATGG CGATGAAGAC TTTGAGTTTT CTGCAGACAG CGCCTCTAGC 
AGTGAAAATA CCGGTCAGTT CCCCAGAAGA GTCATAAACA CGACTGGAGC TGGTGTGGAT 
AATCCAGCAT ATATCCCTGA ACTGGACACC TCTCCATCCA TGCGAAACCC ACCCTGGCAA 
ACAGAGACAG GCCACAACAC AGCTGTAGCA CCTTCTCAGA GAGCTCAGGG CCGACTGCCC 
TGGACGCCCA CTAACCTGAG ACGTCTGGCG CCGCTGAGAG TGAGCAGTCG CTCCACAGAC 
TCCTTCGCCA TGCCTGAACC TGCAGCTGAC GAGGAGACGC CTGAGGAAAA ACCTGCAACA 
CATCATACTA GACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323799.1
CDS	210..2747
Translation	MRAFLVVSGALHEAAAGLDFYGAEGKQNYSSRSSAEGSASSGNSSHSATITTLPIVTWKWHHVETPYLVALWILTCWLCKLVTELNHNITSVIPESGLLIILGFILGGIVWGADKAQTFKLIPVNFFYYLLPQIILDASYCMPNKLFFGNLGAILVHAIIGTCWNAGTVGIALWACYEGGAMGTLNIGCLQFLLFGALMSAVDPVAVIAVFEEVHVNEVLYILVFGESLLNDGVTVVLYNVFDAFVSLGGPKINAAEIIKGIVSFAVVAFGGSFLGVVFAVLTSMLTRVTKNVQIIEAGFIIVLGYLSYLTAEMLSLSAILSLTFCGVCCQKYINANMDEKSVTCLRYSLKVLANGSETMIFVFLGVSAIDQTIWVWNTGFILLTLLFIVVFRMMGVFFLNWILNQSRLIPIDLTDQLVMGYGGLRGAVAYGLAASLDENKIPEKNLMLGTTLIVVYFTVILQGITMKPLVNWLKVKKATQSDLTLNGKLNNRVFEHTLTGMEDICGRMGDNWWTRHWNHFEEKYVCWLLMTSEARKRNDKILDAFHKLNVEDATKYVNEGESKGSLAFIRSYDSASVDFKKKLALEYADIIPDIMADMSEYDFDIDSVPVTSVMKNPIPSVSLDIHEQERRGMSNDLNAHHLLEQHLYKSRRNNRATYSRSHYNTSENPDEMQEIFQRTMRNRLESFKSAKMGVNPAKKVSKHPKKDAPHKPNGKPADPNRDHLYGDEDFEFSADSASSSENTGQFPRRVINTTGAGVDNPAYIPELDTSPSMRNPPWQTETGHNTAVAPSQRAQGRLPWTPTNLRRLAPLRVSSRSTDSFAMPEPAADEETPEEKPATHHTRL

Target ORF information:

RefSeq Version	XM_021468124.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 (slc9a3.2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468124.1

ORF Insert Sequence:

ATGAGAGCAT TTTTGGTGGT CTCCGGTGCA TTACATGAGG CTGCTGCGGG GTTAGATTTC 
TATGGTGCTG AAGGAAAGCA GAATTACTCT TCAAGATCTT CAGCTGAAGG TTCTGCTTCT 
TCAGGCAACA GCAGTCATTC TGCCACTATC ACCACATTAC CCATCGTCAC CTGGAAATGG 
CATCATGTAG AGACTCCATA CCTGGTGGCC CTGTGGATTC TGACCTGCTG GCTCTGTAAA 
TTAGTCACTG AGCTGAACCA CAACATCACC AGTGTGATTC CGGAAAGTGG TCTTCTCATC 
ATCCTGGGCT TTATTTTGGG AGGAATCGTC TGGGGAGCAG ACAAAGCGCA GACTTTTAAA 
CTCATCCCTG TTAACTTCTT CTATTACCTG CTGCCCCAGA TCATCCTGGA TGCCAGTTAC 
TGCATGCCAA ACAAACTATT CTTCGGCAAC CTGGGAGCCA TTCTTGTCCA TGCCATTATT 
GGGACGTGTT GGAATGCGGG CACAGTTGGC ATTGCTTTAT GGGCTTGCTA TGAAGGGGGC 
GCAATGGGGA CCTTGAACAT CGGCTGTCTG CAGTTCCTGC TGTTTGGAGC CCTGATGTCT 
GCTGTGGACC CTGTGGCTGT GATCGCTGTG TTTGAAGAAG TGCATGTGAA TGAAGTGCTT 
TACATCCTGG TGTTTGGAGA GTCACTGCTC AATGATGGAG TCACAGTGGT GCTTTATAAT 
GTGTTTGATG CATTTGTGTC TCTGGGAGGC CCAAAGATCA ATGCTGCAGA GATCATTAAA 
GGAATCGTTT CATTCGCTGT GGTGGCTTTT GGAGGTTCAT TTCTCGGTGT TGTGTTTGCC 
GTCCTGACAT CCATGCTGAC TCGGGTCACC AAAAACGTCC AGATCATTGA AGCCGGTTTC 
ATCATCGTGC TGGGCTATCT GTCTTACCTG ACGGCTGAGA TGCTCTCACT TTCTGCCATC 
CTTTCACTGA CTTTCTGCGG TGTGTGCTGT CAGAAATACA TCAATGCCAA CATGGATGAG 
AAATCCGTCA CATGCCTGCG TTACTCTCTA AAAGTGTTGG CCAACGGCTC CGAGACCATG 
ATCTTCGTTT TCTTGGGAGT TTCGGCCATT GATCAAACCA TCTGGGTTTG GAACACAGGA 
TTCATCCTCC TCACCCTCCT CTTCATCGTT GTATTCAGGA TGATGGGTGT TTTCTTCCTC 
AACTGGATCC TCAATCAGTC TAGACTGATC CCTATAGACC TCACTGATCA GTTGGTAATG 
GGCTACGGTG GGCTGCGAGG AGCAGTGGCG TACGGACTCG CAGCCTCTTT AGATGAGAAC 
AAGATCCCGG AGAAGAATCT AATGCTTGGC ACTACACTCA TTGTAGTGTA TTTCACTGTC 
ATTCTGCAGG GTATCACAAT GAAGCCATTG GTTAATTGGC TAAAAGTTAA AAAGGCAACG 
CAGTCCGATC TGACACTTAA CGGAAAACTG AACAACAGGG TTTTTGAACA CACATTGACA 
GGAATGGAGG ACATCTGTGG CCGAATGGGA GATAACTGGT GGACGAGACA TTGGAACCAT 
TTTGAAGAGA AATATGTCTG CTGGCTTCTA ATGACGAGTG AGGCCAGAAA GCGCAATGAC 
AAAATCTTGG ATGCCTTCCA CAAACTAAAT GTTGAGGACG CCACCAAATA TGTCAATGAG 
GGTGAAAGCA AAGGTTCTCT GGCGTTCATT CGTAGTTACG ACAGTGCCTC TGTAGACTTT 
AAGAAGAAGC TTGCTTTGGA GTACGCAGAC ATCATTCCCG ATATCATGGC TGACATGTCC 
GAATATGACT TTGACATTGA CAGTGTTCCC GTGACCTCTG TCATGAAGAA CCCGATTCCA 
TCTGTATCTC TGGATATCCA TGAGCAGGAG CGGAGGGGCA TGTCAAACGA CCTCAACGCC 
CATCATCTAC TAGAGCAGCA CTTGTACAAG AGCAGGAGAA ATAACCGTGC CACTTACAGC 
CGCAGTCACT ACAACACCTC AGAGAATCCA GACGAAATGC AGGAGATCTT CCAGAGAACC 
ATGAGGAACC GTCTGGAGTC CTTTAAGTCA GCTAAGATGG GAGTCAACCC TGCCAAAAAA 
GTCTCTAAAC ATCCCAAGAA GGATGCACCA CATAAGCCTA ATGGAAAGCC AGCAGACCCA 
AACAGAGACC ATCTTTATGG CGATGAAGAC TTTGAGTTTT CTGCAGACAG CGCCTCTAGC 
AGTGAAAATA CCGGTCAGTT CCCCAGAAGA GTCATAAACA CGACTGGAGC TGGTGTGGAT 
AATCCAGCAT ATATCCCTGA ACTGGACACC TCTCCATCCA TGCGAAACCC ACCCTGGCAA 
ACAGAGACAG GCCACAACAC AGCTGTAGCA CCTTCTCAGA GAGCTCAGGG CCGACTGCCC 
TGGACGCCCA CTAACCTGAG ACGTCTGGCG CCGCTGAGAG TGAGCAGTCG CTCCACAGAC 
TCCTTCGCCA TGCCTGAACC TGCAGCTGAC GAGGAGACGC CTGAGGAAAA ACCTGCAACA 
CATCATACTA GACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc9a3.2 ( XM_009294056.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292331.1 Danio rerio solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 (slc9a3.2), transcript variant X1, mRNA.

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CloneID	ODa45399
Clone ID Related Accession (Same CDS sequence)	XM_021468124.1 , XM_009294056.2 , XM_009294056.3
Accession Version	XM_009294056.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294056.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCAT TTTTGGTGGT CTCCGGTGCA TTACATGAGG CTGCTGCGGG GTTAGATTTC 
TATGGTGCTG AAGGAAAGCA GAATTACTCT TCAAGATCTT CAGCTGAAGG TTCTGCTTCT 
TCAGGCAACA GCAGTCATTC TGCCACTATC ACCACATTAC CCATCGTCAC CTGGAAATGG 
CATCATGTAG AGACTCCATA CCTGGTGGCC CTGTGGATTC TGACCTGCTG GCTCTGTAAA 
TTAGTCACTG AGCTGAACCA CAACATCACC AGTGTGATTC CGGAAAGTGG TCTTCTCATC 
ATCCTGGGCT TTATTTTGGG AGGAATCGTC TGGGGAGCAG ACAAAGCGCA GACTTTTAAA 
CTCATCCCTG TTAACTTCTT CTATTACCTG CTGCCCCAGA TCATCCTGGA TGCCAGTTAC 
TGCATGCCAA ACAAACTATT CTTCGGCAAC CTGGGAGCCA TTCTTGTCCA TGCCATTATT 
GGGACGTGTT GGAATGCGGG CACAGTTGGC ATTGCTTTAT GGGCTTGCTA TGAAGGGGGC 
GCAATGGGGA CCTTGAACAT CGGCTGTCTG CAGTTCCTGC TGTTTGGAGC CCTGATGTCT 
GCTGTGGACC CTGTGGCTGT GATCGCTGTG TTTGAAGAAG TGCATGTGAA TGAAGTGCTT 
TACATCCTGG TGTTTGGAGA GTCACTGCTC AATGATGGAG TCACAGTGGT GCTTTATAAT 
GTGTTTGATG CATTTGTGTC TCTGGGAGGC CCAAAGATCA ATGCTGCAGA GATCATTAAA 
GGAATCGTTT CATTCGCTGT GGTGGCTTTT GGAGGTTCAT TTCTCGGTGT TGTGTTTGCC 
GTCCTGACAT CCATGCTGAC TCGGGTCACC AAAAACGTCC AGATCATTGA AGCCGGTTTC 
ATCATCGTGC TGGGCTATCT GTCTTACCTG ACGGCTGAGA TGCTCTCACT TTCTGCCATC 
CTTTCACTGA CTTTCTGCGG TGTGTGCTGT CAGAAATACA TCAATGCCAA CATGGATGAG 
AAATCCGTCA CATGCCTGCG TTACTCTCTA AAAGTGTTGG CCAACGGCTC CGAGACCATG 
ATCTTCGTTT TCTTGGGAGT TTCGGCCATT GATCAAACCA TCTGGGTTTG GAACACAGGA 
TTCATCCTCC TCACCCTCCT CTTCATCGTT GTATTCAGGA TGATGGGTGT TTTCTTCCTC 
AACTGGATCC TCAATCAGTC TAGACTGATC CCTATAGACC TCACTGATCA GTTGGTAATG 
GGCTACGGTG GGCTGCGAGG AGCAGTGGCG TACGGACTCG CAGCCTCTTT AGATGAGAAC 
AAGATCCCGG AGAAGAATCT AATGCTTGGC ACTACACTCA TTGTAGTGTA TTTCACTGTC 
ATTCTGCAGG GTATCACAAT GAAGCCATTG GTTAATTGGC TAAAAGTTAA AAAGGCAACG 
CAGTCCGATC TGACACTTAA CGGAAAACTG AACAACAGGG TTTTTGAACA CACATTGACA 
GGAATGGAGG ACATCTGTGG CCGAATGGGA GATAACTGGT GGACGAGACA TTGGAACCAT 
TTTGAAGAGA AATATGTCTG CTGGCTTCTA ATGACGAGTG AGGCCAGAAA GCGCAATGAC 
AAAATCTTGG ATGCCTTCCA CAAACTAAAT GTTGAGGACG CCACCAAATA TGTCAATGAG 
GGTGAAAGCA AAGGTTCTCT GGCGTTCATT CGTAGTTACG ACAGTGCCTC TGTAGACTTT 
AAGAAGAAGC TTGCTTTGGA GTACGCAGAC ATCATTCCCG ATATCATGGC TGACATGTCC 
GAATATGACT TTGACATTGA CAGTGTTCCC GTGACCTCTG TCATGAAGAA CCCGATTCCA 
TCTGTATCTC TGGATATCCA TGAGCAGGAG CGGAGGGGCA TGTCAAACGA CCTCAACGCC 
CATCATCTAC TAGAGCAGCA CTTGTACAAG AGCAGGAGAA ATAACCGTGC CACTTACAGC 
CGCAGTCACT ACAACACCTC AGAGAATCCA GACGAAATGC AGGAGATCTT CCAGAGAACC 
ATGAGGAACC GTCTGGAGTC CTTTAAGTCA GCTAAGATGG GAGTCAACCC TGCCAAAAAA 
GTCTCTAAAC ATCCCAAGAA GGATGCACCA CATAAGCCTA ATGGAAAGCC AGCAGACCCA 
AACAGAGACC ATCTTTATGG CGATGAAGAC TTTGAGTTTT CTGCAGACAG CGCCTCTAGC 
AGTGAAAATA CCGGTCAGTT CCCCAGAAGA GTCATAAACA CGACTGGAGC TGGTGTGGAT 
AATCCAGCAT ATATCCCTGA ACTGGACACC TCTCCATCCA TGCGAAACCC ACCCTGGCAA 
ACAGAGACAG GCCACAACAC AGCTGTAGCA CCTTCTCAGA GAGCTCAGGG CCGACTGCCC 
TGGACGCCCA CTAACCTGAG ACGTCTGGCG CCGCTGAGAG TGAGCAGTCG CTCCACAGAC 
TCCTTCGCCA TGCCTGAACC TGCAGCTGAC GAGGAGACGC CTGAGGAAAA ACCTGCAACA 
CATCATACTA GACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292331.1
CDS	210..2747
Translation	MRAFLVVSGALHEAAAGLDFYGAEGKQNYSSRSSAEGSASSGNSSHSATITTLPIVTWKWHHVETPYLVALWILTCWLCKLVTELNHNITSVIPESGLLIILGFILGGIVWGADKAQTFKLIPVNFFYYLLPQIILDASYCMPNKLFFGNLGAILVHAIIGTCWNAGTVGIALWACYEGGAMGTLNIGCLQFLLFGALMSAVDPVAVIAVFEEVHVNEVLYILVFGESLLNDGVTVVLYNVFDAFVSLGGPKINAAEIIKGIVSFAVVAFGGSFLGVVFAVLTSMLTRVTKNVQIIEAGFIIVLGYLSYLTAEMLSLSAILSLTFCGVCCQKYINANMDEKSVTCLRYSLKVLANGSETMIFVFLGVSAIDQTIWVWNTGFILLTLLFIVVFRMMGVFFLNWILNQSRLIPIDLTDQLVMGYGGLRGAVAYGLAASLDENKIPEKNLMLGTTLIVVYFTVILQGITMKPLVNWLKVKKATQSDLTLNGKLNNRVFEHTLTGMEDICGRMGDNWWTRHWNHFEEKYVCWLLMTSEARKRNDKILDAFHKLNVEDATKYVNEGESKGSLAFIRSYDSASVDFKKKLALEYADIIPDIMADMSEYDFDIDSVPVTSVMKNPIPSVSLDIHEQERRGMSNDLNAHHLLEQHLYKSRRNNRATYSRSHYNTSENPDEMQEIFQRTMRNRLESFKSAKMGVNPAKKVSKHPKKDAPHKPNGKPADPNRDHLYGDEDFEFSADSASSSENTGQFPRRVINTTGAGVDNPAYIPELDTSPSMRNPPWQTETGHNTAVAPSQRAQGRLPWTPTNLRRLAPLRVSSRSTDSFAMPEPAADEETPEEKPATHHTRL

Target ORF information:

RefSeq Version	XM_009294056.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 (slc9a3.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294056.2

ORF Insert Sequence:

ATGAGAGCAT TTTTGGTGGT CTCCGGTGCA TTACATGAGG CTGCTGCGGG GTTAGATTTC 
TATGGTGCTG AAGGAAAGCA GAATTACTCT TCAAGATCTT CAGCTGAAGG TTCTGCTTCT 
TCAGGCAACA GCAGTCATTC TGCCACTATC ACCACATTAC CCATCGTCAC CTGGAAATGG 
CATCATGTAG AGACTCCATA CCTGGTGGCC CTGTGGATTC TGACCTGCTG GCTCTGTAAA 
TTAGTCACTG AGCTGAACCA CAACATCACC AGTGTGATTC CGGAAAGTGG TCTTCTCATC 
ATCCTGGGCT TTATTTTGGG AGGAATCGTC TGGGGAGCAG ACAAAGCGCA GACTTTTAAA 
CTCATCCCTG TTAACTTCTT CTATTACCTG CTGCCCCAGA TCATCCTGGA TGCCAGTTAC 
TGCATGCCAA ACAAACTATT CTTCGGCAAC CTGGGAGCCA TTCTTGTCCA TGCCATTATT 
GGGACGTGTT GGAATGCGGG CACAGTTGGC ATTGCTTTAT GGGCTTGCTA TGAAGGGGGC 
GCAATGGGGA CCTTGAACAT CGGCTGTCTG CAGTTCCTGC TGTTTGGAGC CCTGATGTCT 
GCTGTGGACC CTGTGGCTGT GATCGCTGTG TTTGAAGAAG TGCATGTGAA TGAAGTGCTT 
TACATCCTGG TGTTTGGAGA GTCACTGCTC AATGATGGAG TCACAGTGGT GCTTTATAAT 
GTGTTTGATG CATTTGTGTC TCTGGGAGGC CCAAAGATCA ATGCTGCAGA GATCATTAAA 
GGAATCGTTT CATTCGCTGT GGTGGCTTTT GGAGGTTCAT TTCTCGGTGT TGTGTTTGCC 
GTCCTGACAT CCATGCTGAC TCGGGTCACC AAAAACGTCC AGATCATTGA AGCCGGTTTC 
ATCATCGTGC TGGGCTATCT GTCTTACCTG ACGGCTGAGA TGCTCTCACT TTCTGCCATC 
CTTTCACTGA CTTTCTGCGG TGTGTGCTGT CAGAAATACA TCAATGCCAA CATGGATGAG 
AAATCCGTCA CATGCCTGCG TTACTCTCTA AAAGTGTTGG CCAACGGCTC CGAGACCATG 
ATCTTCGTTT TCTTGGGAGT TTCGGCCATT GATCAAACCA TCTGGGTTTG GAACACAGGA 
TTCATCCTCC TCACCCTCCT CTTCATCGTT GTATTCAGGA TGATGGGTGT TTTCTTCCTC 
AACTGGATCC TCAATCAGTC TAGACTGATC CCTATAGACC TCACTGATCA GTTGGTAATG 
GGCTACGGTG GGCTGCGAGG AGCAGTGGCG TACGGACTCG CAGCCTCTTT AGATGAGAAC 
AAGATCCCGG AGAAGAATCT AATGCTTGGC ACTACACTCA TTGTAGTGTA TTTCACTGTC 
ATTCTGCAGG GTATCACAAT GAAGCCATTG GTTAATTGGC TAAAAGTTAA AAAGGCAACG 
CAGTCCGATC TGACACTTAA CGGAAAACTG AACAACAGGG TTTTTGAACA CACATTGACA 
GGAATGGAGG ACATCTGTGG CCGAATGGGA GATAACTGGT GGACGAGACA TTGGAACCAT 
TTTGAAGAGA AATATGTCTG CTGGCTTCTA ATGACGAGTG AGGCCAGAAA GCGCAATGAC 
AAAATCTTGG ATGCCTTCCA CAAACTAAAT GTTGAGGACG CCACCAAATA TGTCAATGAG 
GGTGAAAGCA AAGGTTCTCT GGCGTTCATT CGTAGTTACG ACAGTGCCTC TGTAGACTTT 
AAGAAGAAGC TTGCTTTGGA GTACGCAGAC ATCATTCCCG ATATCATGGC TGACATGTCC 
GAATATGACT TTGACATTGA CAGTGTTCCC GTGACCTCTG TCATGAAGAA CCCGATTCCA 
TCTGTATCTC TGGATATCCA TGAGCAGGAG CGGAGGGGCA TGTCAAACGA CCTCAACGCC 
CATCATCTAC TAGAGCAGCA CTTGTACAAG AGCAGGAGAA ATAACCGTGC CACTTACAGC 
CGCAGTCACT ACAACACCTC AGAGAATCCA GACGAAATGC AGGAGATCTT CCAGAGAACC 
ATGAGGAACC GTCTGGAGTC CTTTAAGTCA GCTAAGATGG GAGTCAACCC TGCCAAAAAA 
GTCTCTAAAC ATCCCAAGAA GGATGCACCA CATAAGCCTA ATGGAAAGCC AGCAGACCCA 
AACAGAGACC ATCTTTATGG CGATGAAGAC TTTGAGTTTT CTGCAGACAG CGCCTCTAGC 
AGTGAAAATA CCGGTCAGTT CCCCAGAAGA GTCATAAACA CGACTGGAGC TGGTGTGGAT 
AATCCAGCAT ATATCCCTGA ACTGGACACC TCTCCATCCA TGCGAAACCC ACCCTGGCAA 
ACAGAGACAG GCCACAACAC AGCTGTAGCA CCTTCTCAGA GAGCTCAGGG CCGACTGCCC 
TGGACGCCCA CTAACCTGAG ACGTCTGGCG CCGCTGAGAG TGAGCAGTCG CTCCACAGAC 
TCCTTCGCCA TGCCTGAACC TGCAGCTGAC GAGGAGACGC CTGAGGAAAA ACCTGCAACA 
CATCATACTA GACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc9a3.2 ( XM_009294056.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292331.1 Danio rerio solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 (slc9a3.2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa45399
Clone ID Related Accession (Same CDS sequence)	XM_021468124.1 , XM_009294056.2 , XM_009294056.3
Accession Version	XM_009294056.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGCAT TTTTGGTGGT CTCCGGTGCA TTACATGAGG CTGCTGCGGG GTTAGATTTC 
TATGGTGCTG AAGGAAAGCA GAATTACTCT TCAAGATCTT CAGCTGAAGG TTCTGCTTCT 
TCAGGCAACA GCAGTCATTC TGCCACTATC ACCACATTAC CCATCGTCAC CTGGAAATGG 
CATCATGTAG AGACTCCATA CCTGGTGGCC CTGTGGATTC TGACCTGCTG GCTCTGTAAA 
TTAGTCACTG AGCTGAACCA CAACATCACC AGTGTGATTC CGGAAAGTGG TCTTCTCATC 
ATCCTGGGCT TTATTTTGGG AGGAATCGTC TGGGGAGCAG ACAAAGCGCA GACTTTTAAA 
CTCATCCCTG TTAACTTCTT CTATTACCTG CTGCCCCAGA TCATCCTGGA TGCCAGTTAC 
TGCATGCCAA ACAAACTATT CTTCGGCAAC CTGGGAGCCA TTCTTGTCCA TGCCATTATT 
GGGACGTGTT GGAATGCGGG CACAGTTGGC ATTGCTTTAT GGGCTTGCTA TGAAGGGGGC 
GCAATGGGGA CCTTGAACAT CGGCTGTCTG CAGTTCCTGC TGTTTGGAGC CCTGATGTCT 
GCTGTGGACC CTGTGGCTGT GATCGCTGTG TTTGAAGAAG TGCATGTGAA TGAAGTGCTT 
TACATCCTGG TGTTTGGAGA GTCACTGCTC AATGATGGAG TCACAGTGGT GCTTTATAAT 
GTGTTTGATG CATTTGTGTC TCTGGGAGGC CCAAAGATCA ATGCTGCAGA GATCATTAAA 
GGAATCGTTT CATTCGCTGT GGTGGCTTTT GGAGGTTCAT TTCTCGGTGT TGTGTTTGCC 
GTCCTGACAT CCATGCTGAC TCGGGTCACC AAAAACGTCC AGATCATTGA AGCCGGTTTC 
ATCATCGTGC TGGGCTATCT GTCTTACCTG ACGGCTGAGA TGCTCTCACT TTCTGCCATC 
CTTTCACTGA CTTTCTGCGG TGTGTGCTGT CAGAAATACA TCAATGCCAA CATGGATGAG 
AAATCCGTCA CATGCCTGCG TTACTCTCTA AAAGTGTTGG CCAACGGCTC CGAGACCATG 
ATCTTCGTTT TCTTGGGAGT TTCGGCCATT GATCAAACCA TCTGGGTTTG GAACACAGGA 
TTCATCCTCC TCACCCTCCT CTTCATCGTT GTATTCAGGA TGATGGGTGT TTTCTTCCTC 
AACTGGATCC TCAATCAGTC TAGACTGATC CCTATAGACC TCACTGATCA GTTGGTAATG 
GGCTACGGTG GGCTGCGAGG AGCAGTGGCG TACGGACTCG CAGCCTCTTT AGATGAGAAC 
AAGATCCCGG AGAAGAATCT AATGCTTGGC ACTACACTCA TTGTAGTGTA TTTCACTGTC 
ATTCTGCAGG GTATCACAAT GAAGCCATTG GTTAATTGGC TAAAAGTTAA AAAGGCAACG 
CAGTCCGATC TGACACTTAA CGGAAAACTG AACAACAGGG TTTTTGAACA CACATTGACA 
GGAATGGAGG ACATCTGTGG CCGAATGGGA GATAACTGGT GGACGAGACA TTGGAACCAT 
TTTGAAGAGA AATATGTCTG CTGGCTTCTA ATGACGAGTG AGGCCAGAAA GCGCAATGAC 
AAAATCTTGG ATGCCTTCCA CAAACTAAAT GTTGAGGACG CCACCAAATA TGTCAATGAG 
GGTGAAAGCA AAGGTTCTCT GGCGTTCATT CGTAGTTACG ACAGTGCCTC TGTAGACTTT 
AAGAAGAAGC TTGCTTTGGA GTACGCAGAC ATCATTCCCG ATATCATGGC TGACATGTCC 
GAATATGACT TTGACATTGA CAGTGTTCCC GTGACCTCTG TCATGAAGAA CCCGATTCCA 
TCTGTATCTC TGGATATCCA TGAGCAGGAG CGGAGGGGCA TGTCAAACGA CCTCAACGCC 
CATCATCTAC TAGAGCAGCA CTTGTACAAG AGCAGGAGAA ATAACCGTGC CACTTACAGC 
CGCAGTCACT ACAACACCTC AGAGAATCCA GACGAAATGC AGGAGATCTT CCAGAGAACC 
ATGAGGAACC GTCTGGAGTC CTTTAAGTCA GCTAAGATGG GAGTCAACCC TGCCAAAAAA 
GTCTCTAAAC ATCCCAAGAA GGATGCACCA CATAAGCCTA ATGGAAAGCC AGCAGACCCA 
AACAGAGACC ATCTTTATGG CGATGAAGAC TTTGAGTTTT CTGCAGACAG CGCCTCTAGC 
AGTGAAAATA CCGGTCAGTT CCCCAGAAGA GTCATAAACA CGACTGGAGC TGGTGTGGAT 
AATCCAGCAT ATATCCCTGA ACTGGACACC TCTCCATCCA TGCGAAACCC ACCCTGGCAA 
ACAGAGACAG GCCACAACAC AGCTGTAGCA CCTTCTCAGA GAGCTCAGGG CCGACTGCCC 
TGGACGCCCA CTAACCTGAG ACGTCTGGCG CCGCTGAGAG TGAGCAGTCG CTCCACAGAC 
TCCTTCGCCA TGCCTGAACC TGCAGCTGAC GAGGAGACGC CTGAGGAAAA ACCTGCAACA 
CATCATACTA GACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292331.1
CDS	213..2750
Translation	MRAFLVVSGALHEAAAGLDFYGAEGKQNYSSRSSAEGSASSGNSSHSATITTLPIVTWKWHHVETPYLVALWILTCWLCKLVTELNHNITSVIPESGLLIILGFILGGIVWGADKAQTFKLIPVNFFYYLLPQIILDASYCMPNKLFFGNLGAILVHAIIGTCWNAGTVGIALWACYEGGAMGTLNIGCLQFLLFGALMSAVDPVAVIAVFEEVHVNEVLYILVFGESLLNDGVTVVLYNVFDAFVSLGGPKINAAEIIKGIVSFAVVAFGGSFLGVVFAVLTSMLTRVTKNVQIIEAGFIIVLGYLSYLTAEMLSLSAILSLTFCGVCCQKYINANMDEKSVTCLRYSLKVLANGSETMIFVFLGVSAIDQTIWVWNTGFILLTLLFIVVFRMMGVFFLNWILNQSRLIPIDLTDQLVMGYGGLRGAVAYGLAASLDENKIPEKNLMLGTTLIVVYFTVILQGITMKPLVNWLKVKKATQSDLTLNGKLNNRVFEHTLTGMEDICGRMGDNWWTRHWNHFEEKYVCWLLMTSEARKRNDKILDAFHKLNVEDATKYVNEGESKGSLAFIRSYDSASVDFKKKLALEYADIIPDIMADMSEYDFDIDSVPVTSVMKNPIPSVSLDIHEQERRGMSNDLNAHHLLEQHLYKSRRNNRATYSRSHYNTSENPDEMQEIFQRTMRNRLESFKSAKMGVNPAKKVSKHPKKDAPHKPNGKPADPNRDHLYGDEDFEFSADSASSSENTGQFPRRVINTTGAGVDNPAYIPELDTSPSMRNPPWQTETGHNTAVAPSQRAQGRLPWTPTNLRRLAPLRVSSRSTDSFAMPEPAADEETPEEKPATHHTRL

Target ORF information:

RefSeq Version	XM_009294056.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3, tandem duplicate 2 (slc9a3.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294056.3

ORF Insert Sequence:

ATGAGAGCAT TTTTGGTGGT CTCCGGTGCA TTACATGAGG CTGCTGCGGG GTTAGATTTC 
TATGGTGCTG AAGGAAAGCA GAATTACTCT TCAAGATCTT CAGCTGAAGG TTCTGCTTCT 
TCAGGCAACA GCAGTCATTC TGCCACTATC ACCACATTAC CCATCGTCAC CTGGAAATGG 
CATCATGTAG AGACTCCATA CCTGGTGGCC CTGTGGATTC TGACCTGCTG GCTCTGTAAA 
TTAGTCACTG AGCTGAACCA CAACATCACC AGTGTGATTC CGGAAAGTGG TCTTCTCATC 
ATCCTGGGCT TTATTTTGGG AGGAATCGTC TGGGGAGCAG ACAAAGCGCA GACTTTTAAA 
CTCATCCCTG TTAACTTCTT CTATTACCTG CTGCCCCAGA TCATCCTGGA TGCCAGTTAC 
TGCATGCCAA ACAAACTATT CTTCGGCAAC CTGGGAGCCA TTCTTGTCCA TGCCATTATT 
GGGACGTGTT GGAATGCGGG CACAGTTGGC ATTGCTTTAT GGGCTTGCTA TGAAGGGGGC 
GCAATGGGGA CCTTGAACAT CGGCTGTCTG CAGTTCCTGC TGTTTGGAGC CCTGATGTCT 
GCTGTGGACC CTGTGGCTGT GATCGCTGTG TTTGAAGAAG TGCATGTGAA TGAAGTGCTT 
TACATCCTGG TGTTTGGAGA GTCACTGCTC AATGATGGAG TCACAGTGGT GCTTTATAAT 
GTGTTTGATG CATTTGTGTC TCTGGGAGGC CCAAAGATCA ATGCTGCAGA GATCATTAAA 
GGAATCGTTT CATTCGCTGT GGTGGCTTTT GGAGGTTCAT TTCTCGGTGT TGTGTTTGCC 
GTCCTGACAT CCATGCTGAC TCGGGTCACC AAAAACGTCC AGATCATTGA AGCCGGTTTC 
ATCATCGTGC TGGGCTATCT GTCTTACCTG ACGGCTGAGA TGCTCTCACT TTCTGCCATC 
CTTTCACTGA CTTTCTGCGG TGTGTGCTGT CAGAAATACA TCAATGCCAA CATGGATGAG 
AAATCCGTCA CATGCCTGCG TTACTCTCTA AAAGTGTTGG CCAACGGCTC CGAGACCATG 
ATCTTCGTTT TCTTGGGAGT TTCGGCCATT GATCAAACCA TCTGGGTTTG GAACACAGGA 
TTCATCCTCC TCACCCTCCT CTTCATCGTT GTATTCAGGA TGATGGGTGT TTTCTTCCTC 
AACTGGATCC TCAATCAGTC TAGACTGATC CCTATAGACC TCACTGATCA GTTGGTAATG 
GGCTACGGTG GGCTGCGAGG AGCAGTGGCG TACGGACTCG CAGCCTCTTT AGATGAGAAC 
AAGATCCCGG AGAAGAATCT AATGCTTGGC ACTACACTCA TTGTAGTGTA TTTCACTGTC 
ATTCTGCAGG GTATCACAAT GAAGCCATTG GTTAATTGGC TAAAAGTTAA AAAGGCAACG 
CAGTCCGATC TGACACTTAA CGGAAAACTG AACAACAGGG TTTTTGAACA CACATTGACA 
GGAATGGAGG ACATCTGTGG CCGAATGGGA GATAACTGGT GGACGAGACA TTGGAACCAT 
TTTGAAGAGA AATATGTCTG CTGGCTTCTA ATGACGAGTG AGGCCAGAAA GCGCAATGAC 
AAAATCTTGG ATGCCTTCCA CAAACTAAAT GTTGAGGACG CCACCAAATA TGTCAATGAG 
GGTGAAAGCA AAGGTTCTCT GGCGTTCATT CGTAGTTACG ACAGTGCCTC TGTAGACTTT 
AAGAAGAAGC TTGCTTTGGA GTACGCAGAC ATCATTCCCG ATATCATGGC TGACATGTCC 
GAATATGACT TTGACATTGA CAGTGTTCCC GTGACCTCTG TCATGAAGAA CCCGATTCCA 
TCTGTATCTC TGGATATCCA TGAGCAGGAG CGGAGGGGCA TGTCAAACGA CCTCAACGCC 
CATCATCTAC TAGAGCAGCA CTTGTACAAG AGCAGGAGAA ATAACCGTGC CACTTACAGC 
CGCAGTCACT ACAACACCTC AGAGAATCCA GACGAAATGC AGGAGATCTT CCAGAGAACC 
ATGAGGAACC GTCTGGAGTC CTTTAAGTCA GCTAAGATGG GAGTCAACCC TGCCAAAAAA 
GTCTCTAAAC ATCCCAAGAA GGATGCACCA CATAAGCCTA ATGGAAAGCC AGCAGACCCA 
AACAGAGACC ATCTTTATGG CGATGAAGAC TTTGAGTTTT CTGCAGACAG CGCCTCTAGC 
AGTGAAAATA CCGGTCAGTT CCCCAGAAGA GTCATAAACA CGACTGGAGC TGGTGTGGAT 
AATCCAGCAT ATATCCCTGA ACTGGACACC TCTCCATCCA TGCGAAACCC ACCCTGGCAA 
ACAGAGACAG GCCACAACAC AGCTGTAGCA CCTTCTCAGA GAGCTCAGGG CCGACTGCCC 
TGGACGCCCA CTAACCTGAG ACGTCTGGCG CCGCTGAGAG TGAGCAGTCG CTCCACAGAC 
TCCTTCGCCA TGCCTGAACC TGCAGCTGAC GAGGAGACGC CTGAGGAAAA ACCTGCAACA 
CATCATACTA GACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A role for sodium-chloride cotransporters in the rapid regulation of ion uptake following acute environmental acidosis: new insights from the zebrafish model.
American journal of physiology. Cell physiology311(6)C931-C941(2016 Dec)
Kwong RW,Perry SF

Oestrogen-related receptor ? is required for transepithelial H+ secretion in zebrafish.
Proceedings. Biological sciences283(1825)20152582(2016 Feb)
Guh YJ,Yang CY,Liu ST,Huang CJ,Hwang PP

The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models.
Scientific reports618597(2016 Jan)
Shu Y,Lou Q,Dai Z,Dai X,He J,Hu W,Yin Z

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slc9a3.2 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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