pfkmb cDNA ORF clone, Danio rerio(zebrafish)

The following pfkmb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pfkmb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa36293 NM_001089417.1
Latest version!
Danio rerio phosphofructokinase, muscle b (pfkmb), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa45302 NM_001365336.1
Latest version!
Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa45302 XM_009302553.2
Latest version!
Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa159716 NM_001089417.2
Latest version!
Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa36293
    Clone ID Related Accession (Same CDS sequence) NM_001089417.1
    Accession Version NM_001089417.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-07-02
    Organism Danio rerio(zebrafish)
    Product phosphofructokinase, muscle b
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC139532.1. On or before May 23, 2007 this sequence version replaced XM_001336133.1, XM_691312.2. ##Evidence-Data-START## Transcript exon combination :: BC139532.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGCATT CGGCACCTGT GGACCCCACA AAGATGGGGG TTGGAAGGGC TATTGCTGTC 
    CTCACCTCTG GAGGAGATGC ACAGGGTATG AATGCTGCTG TTCGAGCCAC GGTGAGAGTT
    GGGATCTACA CTGGTGCAAA GGTTTATTTC GTGCATGAGG GCTATCAGGG CCTGGTTGAT
    GGCGGAGATA ATATCCGACA AGCCACTTGG GAGAGCGTGT CAATGATGCT GCAGCTGGGT
    GGCACAGTCA TTGGCAGTGC CCGCTGTCAG GACTTCCGCA CTAAAGAGGG TCGAGCTAAA
    GCCGCTTGTA ACCTGGTGAA GCTGGGCATC ACTAATCTGT GTGTGATCGG TGGAGACGGC
    AGTCTGACCG GTGCCAACGA GTTCCGCAAT GAATGGAGTG AACTGCTCCA GATCTTGCAG
    AAAGCAGGAA AGATCACAGC AGAGGAGGCC AAATGTTCAT CTCATCTAAA CATCGTTGGC
    ATGGTTGGAT CCATAGACAA CGATTTCTGT GGCACGGACA TGACAATCGG CACAGATTCA
    GCTCTGCATA GGATCATGGA GGTAGTGGAT GCCATCACTA CAACAGCTCA GAGTCATCAA
    AGAGCCTTCA TTCTGGAAGT GATGGGGAGG CATTGTGGGT ATTTGGCCTT AGTCACTGCC
    CTTTCATGTG GTGCTGACTG GGTATTCATC CCCGAAATGC CCCCAGATGA AGGATGGGAG
    GACCATCTGT GCAGGAGACT CACTTACCAA AGAAGCATTG GCAATCGTTT GAATGTCATC
    ATTGTGGCCG AAGGAGCTTT GGATCGACAC GGCAAACCCA TCACCTGTGA CATTATCAAG
    AACCTGGTGA CTAAGAAGCT TGGTTTTGAC ACTCGGGCCA CTATTTTGGG CCACGTGCAG
    AGAGGAGGAA CACCTTCAGC CTTTGACAGA ATATTGGGCA GCAGGATGGG TGTGGAGGCT
    GTGATGGCGC TCCTGGAAGC CACTCCTGAC ACCCCTGCCT GTGTGGTCAG TCTGTCTGGA
    AACATGGCTG TTCGTCTGCC CCTCATGGAG TGTGTGCAAG TGACAAAAGA GGTGACCAAA
    GCCATGGCCG AGGGCAGATT TGAGGAAGCT ATTAAGCTCA GGGGAAAGAG TTTTGAGAAC
    AATTGGAACA CATATAAACT CTTAGCCCAT GTGACGGCCC CAGATGTGAA GAGTAATATA
    AACATAGCTA TTCTGAATGT GGGAGCTCCG TGTGCAGGAA TGAACGCAGC GGTCCGTTCA
    GCAGTCAGGA TAGGCATCCT TCAAGGACAC AACATGCTGG CTGTTCATGA CGGCTTCGAG
    GGACTCGCAA ATGGAACTAT TGAACCAATG GCATGGGGTT ATGTGGGAGG CTGGACGGGC
    AAAGGTGGAT CTATTTTAGG AACCAAGAGG TCACTGCCGG CTAATATGAT TGAAGAAATC
    AGCCTGAATA TTGCCAAGTT TAACATCCAT GCTCTGGTCA TCATTGGAGG ATTTGAAGCC
    TTTGTTGGAG GCTTGGAATT AGTGACTGCC AGAGAAAAAT ACGAGGAGTT GTGTATTCCC
    CTGGTTGTTA TTCCAGCAAC TGTGTCTAAT AATGTTCCTG GATCAGACTT CAGCGTTGGT
    GCTGACACTG CGCTCAATAC TATCACAACT ACATGCGATA GGATCAAGCA ATCTGCTGCT
    GGAACGAAGC GCAGGGTTTT CATCATTGAA ACAATGGGAG GATACTGTGG TTATCTGGCA
    ACCATGGCAG GACTGGCTGC AGGGGCTGAT GCTGCGTACG TCTTTGAAGA CCCCTTTGGC
    ATTCATGACC TGGAGGCCAA CGTGGAGCAC TTACTGGAGA AGATGAAGAC AACAGTGAAA
    AGAGGCCTCA TCCTCAGAAA TGAGAAATGT AATGCAAACT ACACTACAGA TTTTCTCTTC
    AACCTCTACT CAGAAGAGGG CAAAGGAGTG TTTGACTGCC GCAAAAATGT TCTTGGCCAC
    ATGCAGCAGG GTGGAACCCC TACACCGTTT GACAGAAATT TTGGCACAAA GATGGGAGCT
    AAAGCTGTGC TGTGGCTGAC AGATAAACTG AAGGAGTGTT ACAGACATGG CCGTATCTTT
    GCCAACACGC CGGACTCTGC CTGTGTCCTG GGAATGAGGA AGAGAGCCAT GCTCTTCCAG
    CCTCTCTCTG ATCTCAAGGA GGACACTGAC TTTGAACATC GTATACCGAA GACTGAGTGG
    TGGCTGAAGC TTAGACCCAT TCTCAAGATT CTGGCCAAAT ACAAGATCAG TTTGGACACC
    TCAGAGACTG CTACGATGGA GCATGTTATC AAGAAGAGAG GAGGAGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001082886.1
    CDS65..2395
    Translation

    Target ORF information:

    RefSeq Version NM_001089417.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phosphofructokinase, muscle b (pfkmb), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001089417.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGCATT CGGCACCTGT GGACCCCACA AAGATGGGGG TTGGAAGGGC TATTGCTGTC 
    CTCACCTCTG GAGGAGATGC ACAGGGTATG AATGCTGCTG TTCGAGCCAC GGTGAGAGTT
    GGGATCTACA CTGGTGCAAA GGTTTATTTC GTGCATGAGG GCTATCAGGG CCTGGTTGAT
    GGCGGAGATA ATATCCGACA AGCCACTTGG GAGAGCGTGT CAATGATGCT GCAGCTGGGT
    GGCACAGTCA TTGGCAGTGC CCGCTGTCAG GACTTCCGCA CTAAAGAGGG TCGAGCTAAA
    GCCGCTTGTA ACCTGGTGAA GCTGGGCATC ACTAATCTGT GTGTGATCGG TGGAGACGGC
    AGTCTGACCG GTGCCAACGA GTTCCGCAAT GAATGGAGTG AACTGCTCCA GATCTTGCAG
    AAAGCAGGAA AGATCACAGC AGAGGAGGCC AAATGTTCAT CTCATCTAAA CATCGTTGGC
    ATGGTTGGAT CCATAGACAA CGATTTCTGT GGCACGGACA TGACAATCGG CACAGATTCA
    GCTCTGCATA GGATCATGGA GGTAGTGGAT GCCATCACTA CAACAGCTCA GAGTCATCAA
    AGAGCCTTCA TTCTGGAAGT GATGGGGAGG CATTGTGGGT ATTTGGCCTT AGTCACTGCC
    CTTTCATGTG GTGCTGACTG GGTATTCATC CCCGAAATGC CCCCAGATGA AGGATGGGAG
    GACCATCTGT GCAGGAGACT CACTTACCAA AGAAGCATTG GCAATCGTTT GAATGTCATC
    ATTGTGGCCG AAGGAGCTTT GGATCGACAC GGCAAACCCA TCACCTGTGA CATTATCAAG
    AACCTGGTGA CTAAGAAGCT TGGTTTTGAC ACTCGGGCCA CTATTTTGGG CCACGTGCAG
    AGAGGAGGAA CACCTTCAGC CTTTGACAGA ATATTGGGCA GCAGGATGGG TGTGGAGGCT
    GTGATGGCGC TCCTGGAAGC CACTCCTGAC ACCCCTGCCT GTGTGGTCAG TCTGTCTGGA
    AACATGGCTG TTCGTCTGCC CCTCATGGAG TGTGTGCAAG TGACAAAAGA GGTGACCAAA
    GCCATGGCCG AGGGCAGATT TGAGGAAGCT ATTAAGCTCA GGGGAAAGAG TTTTGAGAAC
    AATTGGAACA CATATAAACT CTTAGCCCAT GTGACGGCCC CAGATGTGAA GAGTAATATA
    AACATAGCTA TTCTGAATGT GGGAGCTCCG TGTGCAGGAA TGAACGCAGC GGTCCGTTCA
    GCAGTCAGGA TAGGCATCCT TCAAGGACAC AACATGCTGG CTGTTCATGA CGGCTTCGAG
    GGACTCGCAA ATGGAACTAT TGAACCAATG GCATGGGGTT ATGTGGGAGG CTGGACGGGC
    AAAGGTGGAT CTATTTTAGG AACCAAGAGG TCACTGCCGG CTAATATGAT TGAAGAAATC
    AGCCTGAATA TTGCCAAGTT TAACATCCAT GCTCTGGTCA TCATTGGAGG ATTTGAAGCC
    TTTGTTGGAG GCTTGGAATT AGTGACTGCC AGAGAAAAAT ACGAGGAGTT GTGTATTCCC
    CTGGTTGTTA TTCCAGCAAC TGTGTCTAAT AATGTTCCTG GATCAGACTT CAGCGTTGGT
    GCTGACACTG CGCTCAATAC TATCACAACT ACATGCGATA GGATCAAGCA ATCTGCTGCT
    GGAACGAAGC GCAGGGTTTT CATCATTGAA ACAATGGGAG GATACTGTGG TTATCTGGCA
    ACCATGGCAG GACTGGCTGC AGGGGCTGAT GCTGCGTACG TCTTTGAAGA CCCCTTTGGC
    ATTCATGACC TGGAGGCCAA CGTGGAGCAC TTACTGGAGA AGATGAAGAC AACAGTGAAA
    AGAGGCCTCA TCCTCAGAAA TGAGAAATGT AATGCAAACT ACACTACAGA TTTTCTCTTC
    AACCTCTACT CAGAAGAGGG CAAAGGAGTG TTTGACTGCC GCAAAAATGT TCTTGGCCAC
    ATGCAGCAGG GTGGAACCCC TACACCGTTT GACAGAAATT TTGGCACAAA GATGGGAGCT
    AAAGCTGTGC TGTGGCTGAC AGATAAACTG AAGGAGTGTT ACAGACATGG CCGTATCTTT
    GCCAACACGC CGGACTCTGC CTGTGTCCTG GGAATGAGGA AGAGAGCCAT GCTCTTCCAG
    CCTCTCTCTG ATCTCAAGGA GGACACTGAC TTTGAACATC GTATACCGAA GACTGAGTGG
    TGGCTGAAGC TTAGACCCAT TCTCAAGATT CTGGCCAAAT ACAAGATCAG TTTGGACACC
    TCAGAGACTG CTACGATGGA GCATGTTATC AAGAAGAGAG GAGGAGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45302
    Clone ID Related Accession (Same CDS sequence) NM_001365336.1 , XM_009302553.2
    Accession Version NM_001365336.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2700bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-20
    Organism Danio rerio(zebrafish)
    Product phosphofructokinase, muscle b isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL954698.9. On Aug 17, 2018 this sequence version replaced XM_009302553.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGACTG TGTGGGACAA TTGCGTGTGT GTCTGCCAGC TGTGTGTATG CGTTTGGGAT 
    GTGTTTGTAT GCGTGTGCCA GATTAGTGTT TGTTTGTTAG AGCTGTGTGT GTGTTTACTA
    CGACTTTCTG TACTGCTGTT CCACTGGATC CCTCATATCG GACTGACGGT GTGGATCTGG
    CAACGCACAC ATAGGAAGAC TCAGTTTGAT GCTAATCATG TTTACGGATC AAAAGATCCA
    AGTTACTGTG ATGAGTCAAA GACTGAAGTA CTTGCTGATG GAAAGACTAC TTCTACAACG
    TGTTTCGATA GTGGCCTGGA TGATCTCAGC TGTTCCTCCG CCACGCACAA TAGCTGCCGT
    CTCTTCGAAA TGCAGCATTC GGCACCTGTG GACCCCACAA AGATGGGGGT TGGAAGGGCT
    ATTGCTGTCC TCACCTCTGG AGGAGATGCA CAGGGTATGA ATGCTGCTGT TCGAGCCACG
    GTGAGAGTTG GGATCTACAC TGGTGCAAAG GTTTATTTCG TGCATGAGGG CTATCAGGGC
    CTGGTTGATG GCGGAGATAA TATCCGACAA GCCACTTGGG AGAGCGTGTC AATGATGCTG
    CAGCTGGGTG GCACAGTCAT TGGCAGTGCC CGCTGTCAGG ACTTCCGCAC TAAAGAGGGT
    CGAGCTAAAG CCGCTTGTAA CCTGGTGAAG CTGGGCATCA CTAATCTGTG TGTGATCGGT
    GGAGATGGCA GTCTGACCGG TGCCAATGAG TTCCGCAATG AGTGGAGTGA ACTGCTCCAG
    ATCTTGCAGA AAACAGGAAA GATCACAGCA GAGGAGGCCA AATGTTCATC TCATCTAAAC
    ATCGTCGGGA TGGTTGGATC CATAGACAAC GATTTCTGTG GCACGGACAT GACAATCGGC
    ACAGATTCAG CTCTGCACAG GATCATGGAG GTAGTGGACG CCATCACTAC AACAGCTCAG
    AGTCATCAAA GAGCCTTCAT TCTGGAAGTG ATGGGGAGGC ATTGTGGGTA TTTGGCCTTA
    GTCACTGCCC TTTCATGTGG TGCTGACTGG GTATTTATCC CCGAAATGCC CCCAGATGAA
    GGATGGGAGG ACCATCTGTG CAGGAGACTC ACTTACCAAA GAAGCATTGG CAATCGTTTG
    AATGTCATCA TTGTGGCCGA AGGAGCTTTG GATCGACACG GCAAACCCAT CACCTGTGAC
    ATTATCAAGA ACCTGGTGAC TAAGAAGCTT GGTTTTGACA CTCGGGCCAC TATTTTGGGC
    CACGTGCAGA GAGGAGGAAC ACCTTCAGCC TTCGACAGAA TATTGGGCAG CAGGATGGGT
    GTGGAGGCTG TGATGGCGCT CCTGGAAGCC ACTCCTGACA CCCCTGCCTG TGTGGTCAGT
    CTGTCTGGAA ACATGGCTGT TCGTCTGCCC CTCATGGAGT GTGTGCAAGT GACAAAAGAG
    GTGACCAAAG CCATGGCCGA GGGCAGATTT GAGGAAGCTA TTAAGCTCAG GGGAAAGAGT
    TTTGAGAACA ATTGGAACAC ATATAAACTC TTAGCCCATG TGACGGCCCC AGATGTGAAG
    AGTAATATAA ACATAGCTAT TCTGAATGTG GGAGCTCCCT GTGCAGGAAT GAACGCAGCA
    GTCCGTTCAG CAGTCAGGAT CGGCATCCTT CAAGGACACA ACATGCTGGC TGTTCATGAC
    GGCTTCGAGG GACTCGCAAA TGGAACTATT GAACCAATGG CATGGGGTTA TGTGGGAGGC
    TGGACGGGCA AAGGTGGATC TATTTTAGGA ACCAAGAGGT CACTGCCGGC TAATATGATT
    GAAGAAATCA GCCTGAATAT AGCCAAGTTT AACATCCATG CCCTGGTCAT CATTGGAGGA
    TTTGAAGCCT TTGTTGGAGG CTTGGAATTA GTGACTGCCA GAGAAAAATA CGAGGAGTTG
    TGTATTCCTC TGGTTGTTAT TCCAGCAACT GTGTCTAATA ATGTTCCTGG ATCAGACTTC
    AGCGTTGGTG CTGATACTGC GCTTAATACT ATCACAACTA CATGCGATAG GATCAAGCAA
    TCTGCTGCTG GAACGAAGCG CAGGGTTTTC ATCATTGAAA CAATGGGAGG ATACTGTGGT
    TATCTGGCAA CCATGGCAGG ACTGGCTGCA GGGGCTGACG CTGCGTACGT CTTTGAAGAC
    CCCTTTGGCA TTCATGACCT GGAGGCCAAC GTGGAGCACT TACTGGAGAA GATGAAGACA
    ACAGTGAAAA GAGGCCTCAT CCTCAGAAAT GAGAAATGTA ATGCAAACTA CACTACAGAT
    TTTCTCTTCA ACCTCTACTC AGAAGAGGGC AAAGGAGTGT TTGACTGCCG CAAAAATGTT
    CTTGGCCACA TGCAGCAGGG TGGAACCCCT ACACCGTTTG ATAGAAATTT TGGCACAAAG
    ATGGGAGCTA AAGCTGTGCT GTGGCTGACA GATAAACTGA AGGAGTGTTA CAGACATGGC
    CGTATCTTTG CCAACACGCC GGACTCTGCC TGTGTCCTGG GAATGAGGAA GAGAGCCATG
    CTCTTCCAGC CTCTCTCTGA TCTCAAGGAG GACACTGACT TTGAACATCG TATACCGAAG
    ACTGAGTGGT GGCTGAAGCT TAGACCCATT CTCAAAATTC TGGCCAAATA CAAGATCAGT
    TTGGACACCT CAGAGACTGC TACGATGGAG CATGTTATCA AGAAGAGAGG AGGAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001352265.1
    CDS223..2922
    Translation

    Target ORF information:

    RefSeq Version NM_001365336.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001365336.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGACTG TGTGGGACAA TTGCGTGTGT GTCTGCCAGC TGTGTGTATG CGTTTGGGAT 
    GTGTTTGTAT GCGTGTGCCA GATTAGTGTT TGTTTGTTAG AGCTGTGTGT GTGTTTACTA
    CGACTTTCTG TACTGCTGTT CCACTGGATC CCTCATATCG GACTGACGGT GTGGATCTGG
    CAACGCACAC ATAGGAAGAC TCAGTTTGAT GCTAATCATG TTTACGGATC AAAAGATCCA
    AGTTACTGTG ATGAGTCAAA GACTGAAGTA CTTGCTGATG GAAAGACTAC TTCTACAACG
    TGTTTCGATA GTGGCCTGGA TGATCTCAGC TGTTCCTCCG CCACGCACAA TAGCTGCCGT
    CTCTTCGAAA TGCAGCATTC GGCACCTGTG GACCCCACAA AGATGGGGGT TGGAAGGGCT
    ATTGCTGTCC TCACCTCTGG AGGAGATGCA CAGGGTATGA ATGCTGCTGT TCGAGCCACG
    GTGAGAGTTG GGATCTACAC TGGTGCAAAG GTTTATTTCG TGCATGAGGG CTATCAGGGC
    CTGGTTGATG GCGGAGATAA TATCCGACAA GCCACTTGGG AGAGCGTGTC AATGATGCTG
    CAGCTGGGTG GCACAGTCAT TGGCAGTGCC CGCTGTCAGG ACTTCCGCAC TAAAGAGGGT
    CGAGCTAAAG CCGCTTGTAA CCTGGTGAAG CTGGGCATCA CTAATCTGTG TGTGATCGGT
    GGAGATGGCA GTCTGACCGG TGCCAATGAG TTCCGCAATG AGTGGAGTGA ACTGCTCCAG
    ATCTTGCAGA AAACAGGAAA GATCACAGCA GAGGAGGCCA AATGTTCATC TCATCTAAAC
    ATCGTCGGGA TGGTTGGATC CATAGACAAC GATTTCTGTG GCACGGACAT GACAATCGGC
    ACAGATTCAG CTCTGCACAG GATCATGGAG GTAGTGGACG CCATCACTAC AACAGCTCAG
    AGTCATCAAA GAGCCTTCAT TCTGGAAGTG ATGGGGAGGC ATTGTGGGTA TTTGGCCTTA
    GTCACTGCCC TTTCATGTGG TGCTGACTGG GTATTTATCC CCGAAATGCC CCCAGATGAA
    GGATGGGAGG ACCATCTGTG CAGGAGACTC ACTTACCAAA GAAGCATTGG CAATCGTTTG
    AATGTCATCA TTGTGGCCGA AGGAGCTTTG GATCGACACG GCAAACCCAT CACCTGTGAC
    ATTATCAAGA ACCTGGTGAC TAAGAAGCTT GGTTTTGACA CTCGGGCCAC TATTTTGGGC
    CACGTGCAGA GAGGAGGAAC ACCTTCAGCC TTCGACAGAA TATTGGGCAG CAGGATGGGT
    GTGGAGGCTG TGATGGCGCT CCTGGAAGCC ACTCCTGACA CCCCTGCCTG TGTGGTCAGT
    CTGTCTGGAA ACATGGCTGT TCGTCTGCCC CTCATGGAGT GTGTGCAAGT GACAAAAGAG
    GTGACCAAAG CCATGGCCGA GGGCAGATTT GAGGAAGCTA TTAAGCTCAG GGGAAAGAGT
    TTTGAGAACA ATTGGAACAC ATATAAACTC TTAGCCCATG TGACGGCCCC AGATGTGAAG
    AGTAATATAA ACATAGCTAT TCTGAATGTG GGAGCTCCCT GTGCAGGAAT GAACGCAGCA
    GTCCGTTCAG CAGTCAGGAT CGGCATCCTT CAAGGACACA ACATGCTGGC TGTTCATGAC
    GGCTTCGAGG GACTCGCAAA TGGAACTATT GAACCAATGG CATGGGGTTA TGTGGGAGGC
    TGGACGGGCA AAGGTGGATC TATTTTAGGA ACCAAGAGGT CACTGCCGGC TAATATGATT
    GAAGAAATCA GCCTGAATAT AGCCAAGTTT AACATCCATG CCCTGGTCAT CATTGGAGGA
    TTTGAAGCCT TTGTTGGAGG CTTGGAATTA GTGACTGCCA GAGAAAAATA CGAGGAGTTG
    TGTATTCCTC TGGTTGTTAT TCCAGCAACT GTGTCTAATA ATGTTCCTGG ATCAGACTTC
    AGCGTTGGTG CTGATACTGC GCTTAATACT ATCACAACTA CATGCGATAG GATCAAGCAA
    TCTGCTGCTG GAACGAAGCG CAGGGTTTTC ATCATTGAAA CAATGGGAGG ATACTGTGGT
    TATCTGGCAA CCATGGCAGG ACTGGCTGCA GGGGCTGACG CTGCGTACGT CTTTGAAGAC
    CCCTTTGGCA TTCATGACCT GGAGGCCAAC GTGGAGCACT TACTGGAGAA GATGAAGACA
    ACAGTGAAAA GAGGCCTCAT CCTCAGAAAT GAGAAATGTA ATGCAAACTA CACTACAGAT
    TTTCTCTTCA ACCTCTACTC AGAAGAGGGC AAAGGAGTGT TTGACTGCCG CAAAAATGTT
    CTTGGCCACA TGCAGCAGGG TGGAACCCCT ACACCGTTTG ATAGAAATTT TGGCACAAAG
    ATGGGAGCTA AAGCTGTGCT GTGGCTGACA GATAAACTGA AGGAGTGTTA CAGACATGGC
    CGTATCTTTG CCAACACGCC GGACTCTGCC TGTGTCCTGG GAATGAGGAA GAGAGCCATG
    CTCTTCCAGC CTCTCTCTGA TCTCAAGGAG GACACTGACT TTGAACATCG TATACCGAAG
    ACTGAGTGGT GGCTGAAGCT TAGACCCATT CTCAAAATTC TGGCCAAATA CAAGATCAGT
    TTGGACACCT CAGAGACTGC TACGATGGAG CATGTTATCA AGAAGAGAGG AGGAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45302
    Clone ID Related Accession (Same CDS sequence) NM_001365336.1 , XM_009302553.2
    Accession Version XM_009302553.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2700bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product phosphofructokinase, muscle b isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGACTG TGTGGGACAA TTGCGTGTGT GTCTGCCAGC TGTGTGTATG CGTTTGGGAT 
    GTGTTTGTAT GCGTGTGCCA GATTAGTGTT TGTTTGTTAG AGCTGTGTGT GTGTTTACTA
    CGACTTTCTG TACTGCTGTT CCACTGGATC CCTCATATCG GACTGACGGT GTGGATCTGG
    CAACGCACAC ATAGGAAGAC TCAGTTTGAT GCTAATCATG TTTACGGATC AAAAGATCCA
    AGTTACTGTG ATGAGTCAAA GACTGAAGTA CTTGCTGATG GAAAGACTAC TTCTACAACG
    TGTTTCGATA GTGGCCTGGA TGATCTCAGC TGTTCCTCCG CCACGCACAA TAGCTGCCGT
    CTCTTCGAAA TGCAGCATTC GGCACCTGTG GACCCCACAA AGATGGGGGT TGGAAGGGCT
    ATTGCTGTCC TCACCTCTGG AGGAGATGCA CAGGGTATGA ATGCTGCTGT TCGAGCCACG
    GTGAGAGTTG GGATCTACAC TGGTGCAAAG GTTTATTTCG TGCATGAGGG CTATCAGGGC
    CTGGTTGATG GCGGAGATAA TATCCGACAA GCCACTTGGG AGAGCGTGTC AATGATGCTG
    CAGCTGGGTG GCACAGTCAT TGGCAGTGCC CGCTGTCAGG ACTTCCGCAC TAAAGAGGGT
    CGAGCTAAAG CCGCTTGTAA CCTGGTGAAG CTGGGCATCA CTAATCTGTG TGTGATCGGT
    GGAGATGGCA GTCTGACCGG TGCCAATGAG TTCCGCAATG AGTGGAGTGA ACTGCTCCAG
    ATCTTGCAGA AAACAGGAAA GATCACAGCA GAGGAGGCCA AATGTTCATC TCATCTAAAC
    ATCGTCGGGA TGGTTGGATC CATAGACAAC GATTTCTGTG GCACGGACAT GACAATCGGC
    ACAGATTCAG CTCTGCACAG GATCATGGAG GTAGTGGACG CCATCACTAC AACAGCTCAG
    AGTCATCAAA GAGCCTTCAT TCTGGAAGTG ATGGGGAGGC ATTGTGGGTA TTTGGCCTTA
    GTCACTGCCC TTTCATGTGG TGCTGACTGG GTATTTATCC CCGAAATGCC CCCAGATGAA
    GGATGGGAGG ACCATCTGTG CAGGAGACTC ACTTACCAAA GAAGCATTGG CAATCGTTTG
    AATGTCATCA TTGTGGCCGA AGGAGCTTTG GATCGACACG GCAAACCCAT CACCTGTGAC
    ATTATCAAGA ACCTGGTGAC TAAGAAGCTT GGTTTTGACA CTCGGGCCAC TATTTTGGGC
    CACGTGCAGA GAGGAGGAAC ACCTTCAGCC TTCGACAGAA TATTGGGCAG CAGGATGGGT
    GTGGAGGCTG TGATGGCGCT CCTGGAAGCC ACTCCTGACA CCCCTGCCTG TGTGGTCAGT
    CTGTCTGGAA ACATGGCTGT TCGTCTGCCC CTCATGGAGT GTGTGCAAGT GACAAAAGAG
    GTGACCAAAG CCATGGCCGA GGGCAGATTT GAGGAAGCTA TTAAGCTCAG GGGAAAGAGT
    TTTGAGAACA ATTGGAACAC ATATAAACTC TTAGCCCATG TGACGGCCCC AGATGTGAAG
    AGTAATATAA ACATAGCTAT TCTGAATGTG GGAGCTCCCT GTGCAGGAAT GAACGCAGCA
    GTCCGTTCAG CAGTCAGGAT CGGCATCCTT CAAGGACACA ACATGCTGGC TGTTCATGAC
    GGCTTCGAGG GACTCGCAAA TGGAACTATT GAACCAATGG CATGGGGTTA TGTGGGAGGC
    TGGACGGGCA AAGGTGGATC TATTTTAGGA ACCAAGAGGT CACTGCCGGC TAATATGATT
    GAAGAAATCA GCCTGAATAT AGCCAAGTTT AACATCCATG CCCTGGTCAT CATTGGAGGA
    TTTGAAGCCT TTGTTGGAGG CTTGGAATTA GTGACTGCCA GAGAAAAATA CGAGGAGTTG
    TGTATTCCTC TGGTTGTTAT TCCAGCAACT GTGTCTAATA ATGTTCCTGG ATCAGACTTC
    AGCGTTGGTG CTGATACTGC GCTTAATACT ATCACAACTA CATGCGATAG GATCAAGCAA
    TCTGCTGCTG GAACGAAGCG CAGGGTTTTC ATCATTGAAA CAATGGGAGG ATACTGTGGT
    TATCTGGCAA CCATGGCAGG ACTGGCTGCA GGGGCTGACG CTGCGTACGT CTTTGAAGAC
    CCCTTTGGCA TTCATGACCT GGAGGCCAAC GTGGAGCACT TACTGGAGAA GATGAAGACA
    ACAGTGAAAA GAGGCCTCAT CCTCAGAAAT GAGAAATGTA ATGCAAACTA CACTACAGAT
    TTTCTCTTCA ACCTCTACTC AGAAGAGGGC AAAGGAGTGT TTGACTGCCG CAAAAATGTT
    CTTGGCCACA TGCAGCAGGG TGGAACCCCT ACACCGTTTG ATAGAAATTT TGGCACAAAG
    ATGGGAGCTA AAGCTGTGCT GTGGCTGACA GATAAACTGA AGGAGTGTTA CAGACATGGC
    CGTATCTTTG CCAACACGCC GGACTCTGCC TGTGTCCTGG GAATGAGGAA GAGAGCCATG
    CTCTTCCAGC CTCTCTCTGA TCTCAAGGAG GACACTGACT TTGAACATCG TATACCGAAG
    ACTGAGTGGT GGCTGAAGCT TAGACCCATT CTCAAAATTC TGGCCAAATA CAAGATCAGT
    TTGGACACCT CAGAGACTGC TACGATGGAG CATGTTATCA AGAAGAGAGG AGGAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300828.1
    CDS223..2922
    Translation

    Target ORF information:

    RefSeq Version XM_009302553.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009302553.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGACTG TGTGGGACAA TTGCGTGTGT GTCTGCCAGC TGTGTGTATG CGTTTGGGAT 
    GTGTTTGTAT GCGTGTGCCA GATTAGTGTT TGTTTGTTAG AGCTGTGTGT GTGTTTACTA
    CGACTTTCTG TACTGCTGTT CCACTGGATC CCTCATATCG GACTGACGGT GTGGATCTGG
    CAACGCACAC ATAGGAAGAC TCAGTTTGAT GCTAATCATG TTTACGGATC AAAAGATCCA
    AGTTACTGTG ATGAGTCAAA GACTGAAGTA CTTGCTGATG GAAAGACTAC TTCTACAACG
    TGTTTCGATA GTGGCCTGGA TGATCTCAGC TGTTCCTCCG CCACGCACAA TAGCTGCCGT
    CTCTTCGAAA TGCAGCATTC GGCACCTGTG GACCCCACAA AGATGGGGGT TGGAAGGGCT
    ATTGCTGTCC TCACCTCTGG AGGAGATGCA CAGGGTATGA ATGCTGCTGT TCGAGCCACG
    GTGAGAGTTG GGATCTACAC TGGTGCAAAG GTTTATTTCG TGCATGAGGG CTATCAGGGC
    CTGGTTGATG GCGGAGATAA TATCCGACAA GCCACTTGGG AGAGCGTGTC AATGATGCTG
    CAGCTGGGTG GCACAGTCAT TGGCAGTGCC CGCTGTCAGG ACTTCCGCAC TAAAGAGGGT
    CGAGCTAAAG CCGCTTGTAA CCTGGTGAAG CTGGGCATCA CTAATCTGTG TGTGATCGGT
    GGAGATGGCA GTCTGACCGG TGCCAATGAG TTCCGCAATG AGTGGAGTGA ACTGCTCCAG
    ATCTTGCAGA AAACAGGAAA GATCACAGCA GAGGAGGCCA AATGTTCATC TCATCTAAAC
    ATCGTCGGGA TGGTTGGATC CATAGACAAC GATTTCTGTG GCACGGACAT GACAATCGGC
    ACAGATTCAG CTCTGCACAG GATCATGGAG GTAGTGGACG CCATCACTAC AACAGCTCAG
    AGTCATCAAA GAGCCTTCAT TCTGGAAGTG ATGGGGAGGC ATTGTGGGTA TTTGGCCTTA
    GTCACTGCCC TTTCATGTGG TGCTGACTGG GTATTTATCC CCGAAATGCC CCCAGATGAA
    GGATGGGAGG ACCATCTGTG CAGGAGACTC ACTTACCAAA GAAGCATTGG CAATCGTTTG
    AATGTCATCA TTGTGGCCGA AGGAGCTTTG GATCGACACG GCAAACCCAT CACCTGTGAC
    ATTATCAAGA ACCTGGTGAC TAAGAAGCTT GGTTTTGACA CTCGGGCCAC TATTTTGGGC
    CACGTGCAGA GAGGAGGAAC ACCTTCAGCC TTCGACAGAA TATTGGGCAG CAGGATGGGT
    GTGGAGGCTG TGATGGCGCT CCTGGAAGCC ACTCCTGACA CCCCTGCCTG TGTGGTCAGT
    CTGTCTGGAA ACATGGCTGT TCGTCTGCCC CTCATGGAGT GTGTGCAAGT GACAAAAGAG
    GTGACCAAAG CCATGGCCGA GGGCAGATTT GAGGAAGCTA TTAAGCTCAG GGGAAAGAGT
    TTTGAGAACA ATTGGAACAC ATATAAACTC TTAGCCCATG TGACGGCCCC AGATGTGAAG
    AGTAATATAA ACATAGCTAT TCTGAATGTG GGAGCTCCCT GTGCAGGAAT GAACGCAGCA
    GTCCGTTCAG CAGTCAGGAT CGGCATCCTT CAAGGACACA ACATGCTGGC TGTTCATGAC
    GGCTTCGAGG GACTCGCAAA TGGAACTATT GAACCAATGG CATGGGGTTA TGTGGGAGGC
    TGGACGGGCA AAGGTGGATC TATTTTAGGA ACCAAGAGGT CACTGCCGGC TAATATGATT
    GAAGAAATCA GCCTGAATAT AGCCAAGTTT AACATCCATG CCCTGGTCAT CATTGGAGGA
    TTTGAAGCCT TTGTTGGAGG CTTGGAATTA GTGACTGCCA GAGAAAAATA CGAGGAGTTG
    TGTATTCCTC TGGTTGTTAT TCCAGCAACT GTGTCTAATA ATGTTCCTGG ATCAGACTTC
    AGCGTTGGTG CTGATACTGC GCTTAATACT ATCACAACTA CATGCGATAG GATCAAGCAA
    TCTGCTGCTG GAACGAAGCG CAGGGTTTTC ATCATTGAAA CAATGGGAGG ATACTGTGGT
    TATCTGGCAA CCATGGCAGG ACTGGCTGCA GGGGCTGACG CTGCGTACGT CTTTGAAGAC
    CCCTTTGGCA TTCATGACCT GGAGGCCAAC GTGGAGCACT TACTGGAGAA GATGAAGACA
    ACAGTGAAAA GAGGCCTCAT CCTCAGAAAT GAGAAATGTA ATGCAAACTA CACTACAGAT
    TTTCTCTTCA ACCTCTACTC AGAAGAGGGC AAAGGAGTGT TTGACTGCCG CAAAAATGTT
    CTTGGCCACA TGCAGCAGGG TGGAACCCCT ACACCGTTTG ATAGAAATTT TGGCACAAAG
    ATGGGAGCTA AAGCTGTGCT GTGGCTGACA GATAAACTGA AGGAGTGTTA CAGACATGGC
    CGTATCTTTG CCAACACGCC GGACTCTGCC TGTGTCCTGG GAATGAGGAA GAGAGCCATG
    CTCTTCCAGC CTCTCTCTGA TCTCAAGGAG GACACTGACT TTGAACATCG TATACCGAAG
    ACTGAGTGGT GGCTGAAGCT TAGACCCATT CTCAAAATTC TGGCCAAATA CAAGATCAGT
    TTGGACACCT CAGAGACTGC TACGATGGAG CATGTTATCA AGAAGAGAGG AGGAGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa159716
    Clone ID Related Accession (Same CDS sequence) NM_001089417.2
    Accession Version NM_001089417.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2331bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-20
    Organism Danio rerio(zebrafish)
    Product phosphofructokinase, muscle b isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL954698.9. On Aug 17, 2018 this sequence version replaced NM_001089417.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC139532.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGCATT CGGCACCTGT GGACCCCACA AAGATGGGGG TTGGAAGGGC TATTGCTGTC 
    CTCACCTCTG GAGGAGATGC ACAGGGTATG AATGCTGCTG TTCGAGCCAC GGTGAGAGTT
    GGGATCTACA CTGGTGCAAA GGTTTATTTC GTGCATGAGG GCTATCAGGG CCTGGTTGAT
    GGCGGAGATA ATATCCGACA AGCCACTTGG GAGAGCGTGT CAATGATGCT GCAGCTGGGT
    GGCACAGTCA TTGGCAGTGC CCGCTGTCAG GACTTCCGCA CTAAAGAGGG TCGAGCTAAA
    GCCGCTTGTA ACCTGGTGAA GCTGGGCATC ACTAATCTGT GTGTGATCGG TGGAGATGGC
    AGTCTGACCG GTGCCAATGA GTTCCGCAAT GAGTGGAGTG AACTGCTCCA GATCTTGCAG
    AAAACAGGAA AGATCACAGC AGAGGAGGCC AAATGTTCAT CTCATCTAAA CATCGTCGGG
    ATGGTTGGAT CCATAGACAA CGATTTCTGT GGCACGGACA TGACAATCGG CACAGATTCA
    GCTCTGCACA GGATCATGGA GGTAGTGGAC GCCATCACTA CAACAGCTCA GAGTCATCAA
    AGAGCCTTCA TTCTGGAAGT GATGGGGAGG CATTGTGGGT ATTTGGCCTT AGTCACTGCC
    CTTTCATGTG GTGCTGACTG GGTATTTATC CCCGAAATGC CCCCAGATGA AGGATGGGAG
    GACCATCTGT GCAGGAGACT CACTTACCAA AGAAGCATTG GCAATCGTTT GAATGTCATC
    ATTGTGGCCG AAGGAGCTTT GGATCGACAC GGCAAACCCA TCACCTGTGA CATTATCAAG
    AACCTGGTGA CTAAGAAGCT TGGTTTTGAC ACTCGGGCCA CTATTTTGGG CCACGTGCAG
    AGAGGAGGAA CACCTTCAGC CTTCGACAGA ATATTGGGCA GCAGGATGGG TGTGGAGGCT
    GTGATGGCGC TCCTGGAAGC CACTCCTGAC ACCCCTGCCT GTGTGGTCAG TCTGTCTGGA
    AACATGGCTG TTCGTCTGCC CCTCATGGAG TGTGTGCAAG TGACAAAAGA GGTGACCAAA
    GCCATGGCCG AGGGCAGATT TGAGGAAGCT ATTAAGCTCA GGGGAAAGAG TTTTGAGAAC
    AATTGGAACA CATATAAACT CTTAGCCCAT GTGACGGCCC CAGATGTGAA GAGTAATATA
    AACATAGCTA TTCTGAATGT GGGAGCTCCC TGTGCAGGAA TGAACGCAGC AGTCCGTTCA
    GCAGTCAGGA TCGGCATCCT TCAAGGACAC AACATGCTGG CTGTTCATGA CGGCTTCGAG
    GGACTCGCAA ATGGAACTAT TGAACCAATG GCATGGGGTT ATGTGGGAGG CTGGACGGGC
    AAAGGTGGAT CTATTTTAGG AACCAAGAGG TCACTGCCGG CTAATATGAT TGAAGAAATC
    AGCCTGAATA TAGCCAAGTT TAACATCCAT GCCCTGGTCA TCATTGGAGG ATTTGAAGCC
    TTTGTTGGAG GCTTGGAATT AGTGACTGCC AGAGAAAAAT ACGAGGAGTT GTGTATTCCT
    CTGGTTGTTA TTCCAGCAAC TGTGTCTAAT AATGTTCCTG GATCAGACTT CAGCGTTGGT
    GCTGATACTG CGCTTAATAC TATCACAACT ACATGCGATA GGATCAAGCA ATCTGCTGCT
    GGAACGAAGC GCAGGGTTTT CATCATTGAA ACAATGGGAG GATACTGTGG TTATCTGGCA
    ACCATGGCAG GACTGGCTGC AGGGGCTGAC GCTGCGTACG TCTTTGAAGA CCCCTTTGGC
    ATTCATGACC TGGAGGCCAA CGTGGAGCAC TTACTGGAGA AGATGAAGAC AACAGTGAAA
    AGAGGCCTCA TCCTCAGAAA TGAGAAATGT AATGCAAACT ACACTACAGA TTTTCTCTTC
    AACCTCTACT CAGAAGAGGG CAAAGGAGTG TTTGACTGCC GCAAAAATGT TCTTGGCCAC
    ATGCAGCAGG GTGGAACCCC TACACCGTTT GATAGAAATT TTGGCACAAA GATGGGAGCT
    AAAGCTGTGC TGTGGCTGAC AGATAAACTG AAGGAGTGTT ACAGACATGG CCGTATCTTT
    GCCAACACGC CGGACTCTGC CTGTGTCCTG GGAATGAGGA AGAGAGCCAT GCTCTTCCAG
    CCTCTCTCTG ATCTCAAGGA GGACACTGAC TTTGAACATC GTATACCGAA GACTGAGTGG
    TGGCTGAAGC TTAGACCCAT TCTCAAAATT CTGGCCAAAT ACAAGATCAG TTTGGACACC
    TCAGAGACTG CTACGATGGA GCATGTTATC AAGAAGAGAG GAGGAGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001082886.2
    CDS78..2408
    Translation

    Target ORF information:

    RefSeq Version NM_001089417.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001089417.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGCATT CGGCACCTGT GGACCCCACA AAGATGGGGG TTGGAAGGGC TATTGCTGTC 
    CTCACCTCTG GAGGAGATGC ACAGGGTATG AATGCTGCTG TTCGAGCCAC GGTGAGAGTT
    GGGATCTACA CTGGTGCAAA GGTTTATTTC GTGCATGAGG GCTATCAGGG CCTGGTTGAT
    GGCGGAGATA ATATCCGACA AGCCACTTGG GAGAGCGTGT CAATGATGCT GCAGCTGGGT
    GGCACAGTCA TTGGCAGTGC CCGCTGTCAG GACTTCCGCA CTAAAGAGGG TCGAGCTAAA
    GCCGCTTGTA ACCTGGTGAA GCTGGGCATC ACTAATCTGT GTGTGATCGG TGGAGATGGC
    AGTCTGACCG GTGCCAATGA GTTCCGCAAT GAGTGGAGTG AACTGCTCCA GATCTTGCAG
    AAAACAGGAA AGATCACAGC AGAGGAGGCC AAATGTTCAT CTCATCTAAA CATCGTCGGG
    ATGGTTGGAT CCATAGACAA CGATTTCTGT GGCACGGACA TGACAATCGG CACAGATTCA
    GCTCTGCACA GGATCATGGA GGTAGTGGAC GCCATCACTA CAACAGCTCA GAGTCATCAA
    AGAGCCTTCA TTCTGGAAGT GATGGGGAGG CATTGTGGGT ATTTGGCCTT AGTCACTGCC
    CTTTCATGTG GTGCTGACTG GGTATTTATC CCCGAAATGC CCCCAGATGA AGGATGGGAG
    GACCATCTGT GCAGGAGACT CACTTACCAA AGAAGCATTG GCAATCGTTT GAATGTCATC
    ATTGTGGCCG AAGGAGCTTT GGATCGACAC GGCAAACCCA TCACCTGTGA CATTATCAAG
    AACCTGGTGA CTAAGAAGCT TGGTTTTGAC ACTCGGGCCA CTATTTTGGG CCACGTGCAG
    AGAGGAGGAA CACCTTCAGC CTTCGACAGA ATATTGGGCA GCAGGATGGG TGTGGAGGCT
    GTGATGGCGC TCCTGGAAGC CACTCCTGAC ACCCCTGCCT GTGTGGTCAG TCTGTCTGGA
    AACATGGCTG TTCGTCTGCC CCTCATGGAG TGTGTGCAAG TGACAAAAGA GGTGACCAAA
    GCCATGGCCG AGGGCAGATT TGAGGAAGCT ATTAAGCTCA GGGGAAAGAG TTTTGAGAAC
    AATTGGAACA CATATAAACT CTTAGCCCAT GTGACGGCCC CAGATGTGAA GAGTAATATA
    AACATAGCTA TTCTGAATGT GGGAGCTCCC TGTGCAGGAA TGAACGCAGC AGTCCGTTCA
    GCAGTCAGGA TCGGCATCCT TCAAGGACAC AACATGCTGG CTGTTCATGA CGGCTTCGAG
    GGACTCGCAA ATGGAACTAT TGAACCAATG GCATGGGGTT ATGTGGGAGG CTGGACGGGC
    AAAGGTGGAT CTATTTTAGG AACCAAGAGG TCACTGCCGG CTAATATGAT TGAAGAAATC
    AGCCTGAATA TAGCCAAGTT TAACATCCAT GCCCTGGTCA TCATTGGAGG ATTTGAAGCC
    TTTGTTGGAG GCTTGGAATT AGTGACTGCC AGAGAAAAAT ACGAGGAGTT GTGTATTCCT
    CTGGTTGTTA TTCCAGCAAC TGTGTCTAAT AATGTTCCTG GATCAGACTT CAGCGTTGGT
    GCTGATACTG CGCTTAATAC TATCACAACT ACATGCGATA GGATCAAGCA ATCTGCTGCT
    GGAACGAAGC GCAGGGTTTT CATCATTGAA ACAATGGGAG GATACTGTGG TTATCTGGCA
    ACCATGGCAG GACTGGCTGC AGGGGCTGAC GCTGCGTACG TCTTTGAAGA CCCCTTTGGC
    ATTCATGACC TGGAGGCCAA CGTGGAGCAC TTACTGGAGA AGATGAAGAC AACAGTGAAA
    AGAGGCCTCA TCCTCAGAAA TGAGAAATGT AATGCAAACT ACACTACAGA TTTTCTCTTC
    AACCTCTACT CAGAAGAGGG CAAAGGAGTG TTTGACTGCC GCAAAAATGT TCTTGGCCAC
    ATGCAGCAGG GTGGAACCCC TACACCGTTT GATAGAAATT TTGGCACAAA GATGGGAGCT
    AAAGCTGTGC TGTGGCTGAC AGATAAACTG AAGGAGTGTT ACAGACATGG CCGTATCTTT
    GCCAACACGC CGGACTCTGC CTGTGTCCTG GGAATGAGGA AGAGAGCCAT GCTCTTCCAG
    CCTCTCTCTG ATCTCAAGGA GGACACTGAC TTTGAACATC GTATACCGAA GACTGAGTGG
    TGGCTGAAGC TTAGACCCAT TCTCAAAATT CTGGCCAAAT ACAAGATCAG TTTGGACACC
    TCAGAGACTG CTACGATGGA GCATGTTATC AAGAAGAGAG GAGGAGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.