pfkmb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pfkmb
Entrez Gene ID	568001
Full Name	phosphofructokinase, muscle b
Synonyms	im:7137110,wu:fc58g11
General protein information	Preferred Names phosphofructokinase, muscle b Names phosphofructokinase, muscle b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001089417.2	NP_001082886.2	phosphofructokinase, muscle b isoform 2
NM_001365336.1	NP_001352265.1	phosphofructokinase, muscle b isoform 1
XM_009302553.2	XP_009300828.1	phosphofructokinase, muscle b isoform X1
NM_001089417.1	NP_001082886.1	phosphofructokinase, muscle b

Pathways from BioSystems

Homologies

Homo sapiens (human)	PFKM	NP_001160158.1
Rattus norvegicus (Norway rat)	Pfkm	NP_113903.1
Mus musculus (house mouse)	Pfkm	NP_067489.3
Xenopus tropicalis (tropical clawed frog)	pfkm	XP_004911968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007642	XP_564589.3
Pan troglodytes (chimpanzee)	PFKM	XP_509029.2
	PFKM	XP_001096244.1
Canis lupus familiaris (dog)	PFKM	NP_001003199.1
Bos taurus (cattle)	PFKM	NP_001068736.1
Drosophila melanogaster (fruit fly)	Pfk	NP_724891.2
Caenorhabditis elegans (roundworm)	Y71H10A.1	NP_741738.1
Gallus gallus (chicken)	PFKM	NP_989554.2
Danio rerio (zebrafish)	pfkmb	NP_001082886.1
Danio rerio (zebrafish)	pfkma	NP_001004575.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Zebrafish embryos as a screen for DNA methylation modifications after compound exposure.
Bouwmeester MC, Ruiter S, Lommelaars T, Sippel J, Hodemaekers HM, van den Brandhof EJ, Pennings JL, Kamstra JH, Jelinek J, Issa JP, Legler J, van der Ven LT
Toxicology and applied pharmacology29184-96(2016 Jan)

Global protein expression profiling of zebrafish organs based on in vivo incorporation of stable isotopes.
Nolte H, Konzer A, Ruhs A, Jungblut B, Braun T, Kr?ger M
Journal of proteome research13(4)2162-74(2014 Apr)

GLUT10 is required for the development of the cardiovascular system and the notochord and connects mitochondrial function to TGF? signaling.
Willaert A, Khatri S, Callewaert BL, Coucke PJ, Crosby SD, Lee JG, Davis EC, Shiva S, Tsang M, De Paepe A, Urban Z
Human molecular genetics21(6)1248-59(2012 Mar)

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pfkmb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pfkmb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36293	NM_001089417.1 Latest version!	Danio rerio phosphofructokinase, muscle b (pfkmb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa45302	NM_001365336.1 Latest version!	Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa45302	XM_009302553.2 Latest version!	Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa159716	NM_001089417.2 Latest version!	Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pfkmb ( NM_001089417.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001082886.1 Danio rerio phosphofructokinase, muscle b (pfkmb), mRNA.

Price & Availability↑

CloneID	ODa36293
Clone ID Related Accession (Same CDS sequence)	NM_001089417.1
Accession Version	NM_001089417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Danio rerio(zebrafish)
Product	phosphofructokinase, muscle b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC139532.1. On or before May 23, 2007 this sequence version replaced XM_001336133.1, XM_691312.2. ##Evidence-Data-START## Transcript exon combination :: BC139532.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCAGCATT CGGCACCTGT GGACCCCACA AAGATGGGGG TTGGAAGGGC TATTGCTGTC 
CTCACCTCTG GAGGAGATGC ACAGGGTATG AATGCTGCTG TTCGAGCCAC GGTGAGAGTT 
GGGATCTACA CTGGTGCAAA GGTTTATTTC GTGCATGAGG GCTATCAGGG CCTGGTTGAT 
GGCGGAGATA ATATCCGACA AGCCACTTGG GAGAGCGTGT CAATGATGCT GCAGCTGGGT 
GGCACAGTCA TTGGCAGTGC CCGCTGTCAG GACTTCCGCA CTAAAGAGGG TCGAGCTAAA 
GCCGCTTGTA ACCTGGTGAA GCTGGGCATC ACTAATCTGT GTGTGATCGG TGGAGACGGC 
AGTCTGACCG GTGCCAACGA GTTCCGCAAT GAATGGAGTG AACTGCTCCA GATCTTGCAG 
AAAGCAGGAA AGATCACAGC AGAGGAGGCC AAATGTTCAT CTCATCTAAA CATCGTTGGC 
ATGGTTGGAT CCATAGACAA CGATTTCTGT GGCACGGACA TGACAATCGG CACAGATTCA 
GCTCTGCATA GGATCATGGA GGTAGTGGAT GCCATCACTA CAACAGCTCA GAGTCATCAA 
AGAGCCTTCA TTCTGGAAGT GATGGGGAGG CATTGTGGGT ATTTGGCCTT AGTCACTGCC 
CTTTCATGTG GTGCTGACTG GGTATTCATC CCCGAAATGC CCCCAGATGA AGGATGGGAG 
GACCATCTGT GCAGGAGACT CACTTACCAA AGAAGCATTG GCAATCGTTT GAATGTCATC 
ATTGTGGCCG AAGGAGCTTT GGATCGACAC GGCAAACCCA TCACCTGTGA CATTATCAAG 
AACCTGGTGA CTAAGAAGCT TGGTTTTGAC ACTCGGGCCA CTATTTTGGG CCACGTGCAG 
AGAGGAGGAA CACCTTCAGC CTTTGACAGA ATATTGGGCA GCAGGATGGG TGTGGAGGCT 
GTGATGGCGC TCCTGGAAGC CACTCCTGAC ACCCCTGCCT GTGTGGTCAG TCTGTCTGGA 
AACATGGCTG TTCGTCTGCC CCTCATGGAG TGTGTGCAAG TGACAAAAGA GGTGACCAAA 
GCCATGGCCG AGGGCAGATT TGAGGAAGCT ATTAAGCTCA GGGGAAAGAG TTTTGAGAAC 
AATTGGAACA CATATAAACT CTTAGCCCAT GTGACGGCCC CAGATGTGAA GAGTAATATA 
AACATAGCTA TTCTGAATGT GGGAGCTCCG TGTGCAGGAA TGAACGCAGC GGTCCGTTCA 
GCAGTCAGGA TAGGCATCCT TCAAGGACAC AACATGCTGG CTGTTCATGA CGGCTTCGAG 
GGACTCGCAA ATGGAACTAT TGAACCAATG GCATGGGGTT ATGTGGGAGG CTGGACGGGC 
AAAGGTGGAT CTATTTTAGG AACCAAGAGG TCACTGCCGG CTAATATGAT TGAAGAAATC 
AGCCTGAATA TTGCCAAGTT TAACATCCAT GCTCTGGTCA TCATTGGAGG ATTTGAAGCC 
TTTGTTGGAG GCTTGGAATT AGTGACTGCC AGAGAAAAAT ACGAGGAGTT GTGTATTCCC 
CTGGTTGTTA TTCCAGCAAC TGTGTCTAAT AATGTTCCTG GATCAGACTT CAGCGTTGGT 
GCTGACACTG CGCTCAATAC TATCACAACT ACATGCGATA GGATCAAGCA ATCTGCTGCT 
GGAACGAAGC GCAGGGTTTT CATCATTGAA ACAATGGGAG GATACTGTGG TTATCTGGCA 
ACCATGGCAG GACTGGCTGC AGGGGCTGAT GCTGCGTACG TCTTTGAAGA CCCCTTTGGC 
ATTCATGACC TGGAGGCCAA CGTGGAGCAC TTACTGGAGA AGATGAAGAC AACAGTGAAA 
AGAGGCCTCA TCCTCAGAAA TGAGAAATGT AATGCAAACT ACACTACAGA TTTTCTCTTC 
AACCTCTACT CAGAAGAGGG CAAAGGAGTG TTTGACTGCC GCAAAAATGT TCTTGGCCAC 
ATGCAGCAGG GTGGAACCCC TACACCGTTT GACAGAAATT TTGGCACAAA GATGGGAGCT 
AAAGCTGTGC TGTGGCTGAC AGATAAACTG AAGGAGTGTT ACAGACATGG CCGTATCTTT 
GCCAACACGC CGGACTCTGC CTGTGTCCTG GGAATGAGGA AGAGAGCCAT GCTCTTCCAG 
CCTCTCTCTG ATCTCAAGGA GGACACTGAC TTTGAACATC GTATACCGAA GACTGAGTGG 
TGGCTGAAGC TTAGACCCAT TCTCAAGATT CTGGCCAAAT ACAAGATCAG TTTGGACACC 
TCAGAGACTG CTACGATGGA GCATGTTATC AAGAAGAGAG GAGGAGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082886.1
CDS	65..2395
Translation	MQHSAPVDPTKMGVGRAIAVLTSGGDAQGMNAAVRATVRVGIYTGAKVYFVHEGYQGLVDGGDNIRQATWESVSMMLQLGGTVIGSARCQDFRTKEGRAKAACNLVKLGITNLCVIGGDGSLTGANEFRNEWSELLQILQKAGKITAEEAKCSSHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIMEVVDAITTTAQSHQRAFILEVMGRHCGYLALVTALSCGADWVFIPEMPPDEGWEDHLCRRLTYQRSIGNRLNVIIVAEGALDRHGKPITCDIIKNLVTKKLGFDTRATILGHVQRGGTPSAFDRILGSRMGVEAVMALLEATPDTPACVVSLSGNMAVRLPLMECVQVTKEVTKAMAEGRFEEAIKLRGKSFENNWNTYKLLAHVTAPDVKSNINIAILNVGAPCAGMNAAVRSAVRIGILQGHNMLAVHDGFEGLANGTIEPMAWGYVGGWTGKGGSILGTKRSLPANMIEEISLNIAKFNIHALVIIGGFEAFVGGLELVTAREKYEELCIPLVVIPATVSNNVPGSDFSVGADTALNTITTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYVFEDPFGIHDLEANVEHLLEKMKTTVKRGLILRNEKCNANYTTDFLFNLYSEEGKGVFDCRKNVLGHMQQGGTPTPFDRNFGTKMGAKAVLWLTDKLKECYRHGRIFANTPDSACVLGMRKRAMLFQPLSDLKEDTDFEHRIPKTEWWLKLRPILKILAKYKISLDTSETATMEHVIKKRGGV

Target ORF information:

RefSeq Version	NM_001089417.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphofructokinase, muscle b (pfkmb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089417.1

ORF Insert Sequence:

ATGCAGCATT CGGCACCTGT GGACCCCACA AAGATGGGGG TTGGAAGGGC TATTGCTGTC 
CTCACCTCTG GAGGAGATGC ACAGGGTATG AATGCTGCTG TTCGAGCCAC GGTGAGAGTT 
GGGATCTACA CTGGTGCAAA GGTTTATTTC GTGCATGAGG GCTATCAGGG CCTGGTTGAT 
GGCGGAGATA ATATCCGACA AGCCACTTGG GAGAGCGTGT CAATGATGCT GCAGCTGGGT 
GGCACAGTCA TTGGCAGTGC CCGCTGTCAG GACTTCCGCA CTAAAGAGGG TCGAGCTAAA 
GCCGCTTGTA ACCTGGTGAA GCTGGGCATC ACTAATCTGT GTGTGATCGG TGGAGACGGC 
AGTCTGACCG GTGCCAACGA GTTCCGCAAT GAATGGAGTG AACTGCTCCA GATCTTGCAG 
AAAGCAGGAA AGATCACAGC AGAGGAGGCC AAATGTTCAT CTCATCTAAA CATCGTTGGC 
ATGGTTGGAT CCATAGACAA CGATTTCTGT GGCACGGACA TGACAATCGG CACAGATTCA 
GCTCTGCATA GGATCATGGA GGTAGTGGAT GCCATCACTA CAACAGCTCA GAGTCATCAA 
AGAGCCTTCA TTCTGGAAGT GATGGGGAGG CATTGTGGGT ATTTGGCCTT AGTCACTGCC 
CTTTCATGTG GTGCTGACTG GGTATTCATC CCCGAAATGC CCCCAGATGA AGGATGGGAG 
GACCATCTGT GCAGGAGACT CACTTACCAA AGAAGCATTG GCAATCGTTT GAATGTCATC 
ATTGTGGCCG AAGGAGCTTT GGATCGACAC GGCAAACCCA TCACCTGTGA CATTATCAAG 
AACCTGGTGA CTAAGAAGCT TGGTTTTGAC ACTCGGGCCA CTATTTTGGG CCACGTGCAG 
AGAGGAGGAA CACCTTCAGC CTTTGACAGA ATATTGGGCA GCAGGATGGG TGTGGAGGCT 
GTGATGGCGC TCCTGGAAGC CACTCCTGAC ACCCCTGCCT GTGTGGTCAG TCTGTCTGGA 
AACATGGCTG TTCGTCTGCC CCTCATGGAG TGTGTGCAAG TGACAAAAGA GGTGACCAAA 
GCCATGGCCG AGGGCAGATT TGAGGAAGCT ATTAAGCTCA GGGGAAAGAG TTTTGAGAAC 
AATTGGAACA CATATAAACT CTTAGCCCAT GTGACGGCCC CAGATGTGAA GAGTAATATA 
AACATAGCTA TTCTGAATGT GGGAGCTCCG TGTGCAGGAA TGAACGCAGC GGTCCGTTCA 
GCAGTCAGGA TAGGCATCCT TCAAGGACAC AACATGCTGG CTGTTCATGA CGGCTTCGAG 
GGACTCGCAA ATGGAACTAT TGAACCAATG GCATGGGGTT ATGTGGGAGG CTGGACGGGC 
AAAGGTGGAT CTATTTTAGG AACCAAGAGG TCACTGCCGG CTAATATGAT TGAAGAAATC 
AGCCTGAATA TTGCCAAGTT TAACATCCAT GCTCTGGTCA TCATTGGAGG ATTTGAAGCC 
TTTGTTGGAG GCTTGGAATT AGTGACTGCC AGAGAAAAAT ACGAGGAGTT GTGTATTCCC 
CTGGTTGTTA TTCCAGCAAC TGTGTCTAAT AATGTTCCTG GATCAGACTT CAGCGTTGGT 
GCTGACACTG CGCTCAATAC TATCACAACT ACATGCGATA GGATCAAGCA ATCTGCTGCT 
GGAACGAAGC GCAGGGTTTT CATCATTGAA ACAATGGGAG GATACTGTGG TTATCTGGCA 
ACCATGGCAG GACTGGCTGC AGGGGCTGAT GCTGCGTACG TCTTTGAAGA CCCCTTTGGC 
ATTCATGACC TGGAGGCCAA CGTGGAGCAC TTACTGGAGA AGATGAAGAC AACAGTGAAA 
AGAGGCCTCA TCCTCAGAAA TGAGAAATGT AATGCAAACT ACACTACAGA TTTTCTCTTC 
AACCTCTACT CAGAAGAGGG CAAAGGAGTG TTTGACTGCC GCAAAAATGT TCTTGGCCAC 
ATGCAGCAGG GTGGAACCCC TACACCGTTT GACAGAAATT TTGGCACAAA GATGGGAGCT 
AAAGCTGTGC TGTGGCTGAC AGATAAACTG AAGGAGTGTT ACAGACATGG CCGTATCTTT 
GCCAACACGC CGGACTCTGC CTGTGTCCTG GGAATGAGGA AGAGAGCCAT GCTCTTCCAG 
CCTCTCTCTG ATCTCAAGGA GGACACTGAC TTTGAACATC GTATACCGAA GACTGAGTGG 
TGGCTGAAGC TTAGACCCAT TCTCAAGATT CTGGCCAAAT ACAAGATCAG TTTGGACACC 
TCAGAGACTG CTACGATGGA GCATGTTATC AAGAAGAGAG GAGGAGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkmb ( NM_001365336.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352265.1 Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa45302
Clone ID Related Accession (Same CDS sequence)	NM_001365336.1 , XM_009302553.2
Accession Version	NM_001365336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-20
Organism	Danio rerio(zebrafish)
Product	phosphofructokinase, muscle b isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL954698.9. On Aug 17, 2018 this sequence version replaced XM_009302553.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGAGACTG TGTGGGACAA TTGCGTGTGT GTCTGCCAGC TGTGTGTATG CGTTTGGGAT 
GTGTTTGTAT GCGTGTGCCA GATTAGTGTT TGTTTGTTAG AGCTGTGTGT GTGTTTACTA 
CGACTTTCTG TACTGCTGTT CCACTGGATC CCTCATATCG GACTGACGGT GTGGATCTGG 
CAACGCACAC ATAGGAAGAC TCAGTTTGAT GCTAATCATG TTTACGGATC AAAAGATCCA 
AGTTACTGTG ATGAGTCAAA GACTGAAGTA CTTGCTGATG GAAAGACTAC TTCTACAACG 
TGTTTCGATA GTGGCCTGGA TGATCTCAGC TGTTCCTCCG CCACGCACAA TAGCTGCCGT 
CTCTTCGAAA TGCAGCATTC GGCACCTGTG GACCCCACAA AGATGGGGGT TGGAAGGGCT 
ATTGCTGTCC TCACCTCTGG AGGAGATGCA CAGGGTATGA ATGCTGCTGT TCGAGCCACG 
GTGAGAGTTG GGATCTACAC TGGTGCAAAG GTTTATTTCG TGCATGAGGG CTATCAGGGC 
CTGGTTGATG GCGGAGATAA TATCCGACAA GCCACTTGGG AGAGCGTGTC AATGATGCTG 
CAGCTGGGTG GCACAGTCAT TGGCAGTGCC CGCTGTCAGG ACTTCCGCAC TAAAGAGGGT 
CGAGCTAAAG CCGCTTGTAA CCTGGTGAAG CTGGGCATCA CTAATCTGTG TGTGATCGGT 
GGAGATGGCA GTCTGACCGG TGCCAATGAG TTCCGCAATG AGTGGAGTGA ACTGCTCCAG 
ATCTTGCAGA AAACAGGAAA GATCACAGCA GAGGAGGCCA AATGTTCATC TCATCTAAAC 
ATCGTCGGGA TGGTTGGATC CATAGACAAC GATTTCTGTG GCACGGACAT GACAATCGGC 
ACAGATTCAG CTCTGCACAG GATCATGGAG GTAGTGGACG CCATCACTAC AACAGCTCAG 
AGTCATCAAA GAGCCTTCAT TCTGGAAGTG ATGGGGAGGC ATTGTGGGTA TTTGGCCTTA 
GTCACTGCCC TTTCATGTGG TGCTGACTGG GTATTTATCC CCGAAATGCC CCCAGATGAA 
GGATGGGAGG ACCATCTGTG CAGGAGACTC ACTTACCAAA GAAGCATTGG CAATCGTTTG 
AATGTCATCA TTGTGGCCGA AGGAGCTTTG GATCGACACG GCAAACCCAT CACCTGTGAC 
ATTATCAAGA ACCTGGTGAC TAAGAAGCTT GGTTTTGACA CTCGGGCCAC TATTTTGGGC 
CACGTGCAGA GAGGAGGAAC ACCTTCAGCC TTCGACAGAA TATTGGGCAG CAGGATGGGT 
GTGGAGGCTG TGATGGCGCT CCTGGAAGCC ACTCCTGACA CCCCTGCCTG TGTGGTCAGT 
CTGTCTGGAA ACATGGCTGT TCGTCTGCCC CTCATGGAGT GTGTGCAAGT GACAAAAGAG 
GTGACCAAAG CCATGGCCGA GGGCAGATTT GAGGAAGCTA TTAAGCTCAG GGGAAAGAGT 
TTTGAGAACA ATTGGAACAC ATATAAACTC TTAGCCCATG TGACGGCCCC AGATGTGAAG 
AGTAATATAA ACATAGCTAT TCTGAATGTG GGAGCTCCCT GTGCAGGAAT GAACGCAGCA 
GTCCGTTCAG CAGTCAGGAT CGGCATCCTT CAAGGACACA ACATGCTGGC TGTTCATGAC 
GGCTTCGAGG GACTCGCAAA TGGAACTATT GAACCAATGG CATGGGGTTA TGTGGGAGGC 
TGGACGGGCA AAGGTGGATC TATTTTAGGA ACCAAGAGGT CACTGCCGGC TAATATGATT 
GAAGAAATCA GCCTGAATAT AGCCAAGTTT AACATCCATG CCCTGGTCAT CATTGGAGGA 
TTTGAAGCCT TTGTTGGAGG CTTGGAATTA GTGACTGCCA GAGAAAAATA CGAGGAGTTG 
TGTATTCCTC TGGTTGTTAT TCCAGCAACT GTGTCTAATA ATGTTCCTGG ATCAGACTTC 
AGCGTTGGTG CTGATACTGC GCTTAATACT ATCACAACTA CATGCGATAG GATCAAGCAA 
TCTGCTGCTG GAACGAAGCG CAGGGTTTTC ATCATTGAAA CAATGGGAGG ATACTGTGGT 
TATCTGGCAA CCATGGCAGG ACTGGCTGCA GGGGCTGACG CTGCGTACGT CTTTGAAGAC 
CCCTTTGGCA TTCATGACCT GGAGGCCAAC GTGGAGCACT TACTGGAGAA GATGAAGACA 
ACAGTGAAAA GAGGCCTCAT CCTCAGAAAT GAGAAATGTA ATGCAAACTA CACTACAGAT 
TTTCTCTTCA ACCTCTACTC AGAAGAGGGC AAAGGAGTGT TTGACTGCCG CAAAAATGTT 
CTTGGCCACA TGCAGCAGGG TGGAACCCCT ACACCGTTTG ATAGAAATTT TGGCACAAAG 
ATGGGAGCTA AAGCTGTGCT GTGGCTGACA GATAAACTGA AGGAGTGTTA CAGACATGGC 
CGTATCTTTG CCAACACGCC GGACTCTGCC TGTGTCCTGG GAATGAGGAA GAGAGCCATG 
CTCTTCCAGC CTCTCTCTGA TCTCAAGGAG GACACTGACT TTGAACATCG TATACCGAAG 
ACTGAGTGGT GGCTGAAGCT TAGACCCATT CTCAAAATTC TGGCCAAATA CAAGATCAGT 
TTGGACACCT CAGAGACTGC TACGATGGAG CATGTTATCA AGAAGAGAGG AGGAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352265.1
CDS	223..2922
Translation	METVWDNCVCVCQLCVCVWDVFVCVCQISVCLLELCVCLLRLSVLLFHWIPHIGLTVWIWQRTHRKTQFDANHVYGSKDPSYCDESKTEVLADGKTTSTTCFDSGLDDLSCSSATHNSCRLFEMQHSAPVDPTKMGVGRAIAVLTSGGDAQGMNAAVRATVRVGIYTGAKVYFVHEGYQGLVDGGDNIRQATWESVSMMLQLGGTVIGSARCQDFRTKEGRAKAACNLVKLGITNLCVIGGDGSLTGANEFRNEWSELLQILQKTGKITAEEAKCSSHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIMEVVDAITTTAQSHQRAFILEVMGRHCGYLALVTALSCGADWVFIPEMPPDEGWEDHLCRRLTYQRSIGNRLNVIIVAEGALDRHGKPITCDIIKNLVTKKLGFDTRATILGHVQRGGTPSAFDRILGSRMGVEAVMALLEATPDTPACVVSLSGNMAVRLPLMECVQVTKEVTKAMAEGRFEEAIKLRGKSFENNWNTYKLLAHVTAPDVKSNINIAILNVGAPCAGMNAAVRSAVRIGILQGHNMLAVHDGFEGLANGTIEPMAWGYVGGWTGKGGSILGTKRSLPANMIEEISLNIAKFNIHALVIIGGFEAFVGGLELVTAREKYEELCIPLVVIPATVSNNVPGSDFSVGADTALNTITTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYVFEDPFGIHDLEANVEHLLEKMKTTVKRGLILRNEKCNANYTTDFLFNLYSEEGKGVFDCRKNVLGHMQQGGTPTPFDRNFGTKMGAKAVLWLTDKLKECYRHGRIFANTPDSACVLGMRKRAMLFQPLSDLKEDTDFEHRIPKTEWWLKLRPILKILAKYKISLDTSETATMEHVIKKRGGV

Target ORF information:

RefSeq Version	NM_001365336.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365336.1

ORF Insert Sequence:

ATGGAGACTG TGTGGGACAA TTGCGTGTGT GTCTGCCAGC TGTGTGTATG CGTTTGGGAT 
GTGTTTGTAT GCGTGTGCCA GATTAGTGTT TGTTTGTTAG AGCTGTGTGT GTGTTTACTA 
CGACTTTCTG TACTGCTGTT CCACTGGATC CCTCATATCG GACTGACGGT GTGGATCTGG 
CAACGCACAC ATAGGAAGAC TCAGTTTGAT GCTAATCATG TTTACGGATC AAAAGATCCA 
AGTTACTGTG ATGAGTCAAA GACTGAAGTA CTTGCTGATG GAAAGACTAC TTCTACAACG 
TGTTTCGATA GTGGCCTGGA TGATCTCAGC TGTTCCTCCG CCACGCACAA TAGCTGCCGT 
CTCTTCGAAA TGCAGCATTC GGCACCTGTG GACCCCACAA AGATGGGGGT TGGAAGGGCT 
ATTGCTGTCC TCACCTCTGG AGGAGATGCA CAGGGTATGA ATGCTGCTGT TCGAGCCACG 
GTGAGAGTTG GGATCTACAC TGGTGCAAAG GTTTATTTCG TGCATGAGGG CTATCAGGGC 
CTGGTTGATG GCGGAGATAA TATCCGACAA GCCACTTGGG AGAGCGTGTC AATGATGCTG 
CAGCTGGGTG GCACAGTCAT TGGCAGTGCC CGCTGTCAGG ACTTCCGCAC TAAAGAGGGT 
CGAGCTAAAG CCGCTTGTAA CCTGGTGAAG CTGGGCATCA CTAATCTGTG TGTGATCGGT 
GGAGATGGCA GTCTGACCGG TGCCAATGAG TTCCGCAATG AGTGGAGTGA ACTGCTCCAG 
ATCTTGCAGA AAACAGGAAA GATCACAGCA GAGGAGGCCA AATGTTCATC TCATCTAAAC 
ATCGTCGGGA TGGTTGGATC CATAGACAAC GATTTCTGTG GCACGGACAT GACAATCGGC 
ACAGATTCAG CTCTGCACAG GATCATGGAG GTAGTGGACG CCATCACTAC AACAGCTCAG 
AGTCATCAAA GAGCCTTCAT TCTGGAAGTG ATGGGGAGGC ATTGTGGGTA TTTGGCCTTA 
GTCACTGCCC TTTCATGTGG TGCTGACTGG GTATTTATCC CCGAAATGCC CCCAGATGAA 
GGATGGGAGG ACCATCTGTG CAGGAGACTC ACTTACCAAA GAAGCATTGG CAATCGTTTG 
AATGTCATCA TTGTGGCCGA AGGAGCTTTG GATCGACACG GCAAACCCAT CACCTGTGAC 
ATTATCAAGA ACCTGGTGAC TAAGAAGCTT GGTTTTGACA CTCGGGCCAC TATTTTGGGC 
CACGTGCAGA GAGGAGGAAC ACCTTCAGCC TTCGACAGAA TATTGGGCAG CAGGATGGGT 
GTGGAGGCTG TGATGGCGCT CCTGGAAGCC ACTCCTGACA CCCCTGCCTG TGTGGTCAGT 
CTGTCTGGAA ACATGGCTGT TCGTCTGCCC CTCATGGAGT GTGTGCAAGT GACAAAAGAG 
GTGACCAAAG CCATGGCCGA GGGCAGATTT GAGGAAGCTA TTAAGCTCAG GGGAAAGAGT 
TTTGAGAACA ATTGGAACAC ATATAAACTC TTAGCCCATG TGACGGCCCC AGATGTGAAG 
AGTAATATAA ACATAGCTAT TCTGAATGTG GGAGCTCCCT GTGCAGGAAT GAACGCAGCA 
GTCCGTTCAG CAGTCAGGAT CGGCATCCTT CAAGGACACA ACATGCTGGC TGTTCATGAC 
GGCTTCGAGG GACTCGCAAA TGGAACTATT GAACCAATGG CATGGGGTTA TGTGGGAGGC 
TGGACGGGCA AAGGTGGATC TATTTTAGGA ACCAAGAGGT CACTGCCGGC TAATATGATT 
GAAGAAATCA GCCTGAATAT AGCCAAGTTT AACATCCATG CCCTGGTCAT CATTGGAGGA 
TTTGAAGCCT TTGTTGGAGG CTTGGAATTA GTGACTGCCA GAGAAAAATA CGAGGAGTTG 
TGTATTCCTC TGGTTGTTAT TCCAGCAACT GTGTCTAATA ATGTTCCTGG ATCAGACTTC 
AGCGTTGGTG CTGATACTGC GCTTAATACT ATCACAACTA CATGCGATAG GATCAAGCAA 
TCTGCTGCTG GAACGAAGCG CAGGGTTTTC ATCATTGAAA CAATGGGAGG ATACTGTGGT 
TATCTGGCAA CCATGGCAGG ACTGGCTGCA GGGGCTGACG CTGCGTACGT CTTTGAAGAC 
CCCTTTGGCA TTCATGACCT GGAGGCCAAC GTGGAGCACT TACTGGAGAA GATGAAGACA 
ACAGTGAAAA GAGGCCTCAT CCTCAGAAAT GAGAAATGTA ATGCAAACTA CACTACAGAT 
TTTCTCTTCA ACCTCTACTC AGAAGAGGGC AAAGGAGTGT TTGACTGCCG CAAAAATGTT 
CTTGGCCACA TGCAGCAGGG TGGAACCCCT ACACCGTTTG ATAGAAATTT TGGCACAAAG 
ATGGGAGCTA AAGCTGTGCT GTGGCTGACA GATAAACTGA AGGAGTGTTA CAGACATGGC 
CGTATCTTTG CCAACACGCC GGACTCTGCC TGTGTCCTGG GAATGAGGAA GAGAGCCATG 
CTCTTCCAGC CTCTCTCTGA TCTCAAGGAG GACACTGACT TTGAACATCG TATACCGAAG 
ACTGAGTGGT GGCTGAAGCT TAGACCCATT CTCAAAATTC TGGCCAAATA CAAGATCAGT 
TTGGACACCT CAGAGACTGC TACGATGGAG CATGTTATCA AGAAGAGAGG AGGAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkmb ( XM_009302553.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300828.1 Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant X1, mRNA.

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CloneID	ODa45302
Clone ID Related Accession (Same CDS sequence)	NM_001365336.1 , XM_009302553.2
Accession Version	XM_009302553.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphofructokinase, muscle b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG TGTGGGACAA TTGCGTGTGT GTCTGCCAGC TGTGTGTATG CGTTTGGGAT 
GTGTTTGTAT GCGTGTGCCA GATTAGTGTT TGTTTGTTAG AGCTGTGTGT GTGTTTACTA 
CGACTTTCTG TACTGCTGTT CCACTGGATC CCTCATATCG GACTGACGGT GTGGATCTGG 
CAACGCACAC ATAGGAAGAC TCAGTTTGAT GCTAATCATG TTTACGGATC AAAAGATCCA 
AGTTACTGTG ATGAGTCAAA GACTGAAGTA CTTGCTGATG GAAAGACTAC TTCTACAACG 
TGTTTCGATA GTGGCCTGGA TGATCTCAGC TGTTCCTCCG CCACGCACAA TAGCTGCCGT 
CTCTTCGAAA TGCAGCATTC GGCACCTGTG GACCCCACAA AGATGGGGGT TGGAAGGGCT 
ATTGCTGTCC TCACCTCTGG AGGAGATGCA CAGGGTATGA ATGCTGCTGT TCGAGCCACG 
GTGAGAGTTG GGATCTACAC TGGTGCAAAG GTTTATTTCG TGCATGAGGG CTATCAGGGC 
CTGGTTGATG GCGGAGATAA TATCCGACAA GCCACTTGGG AGAGCGTGTC AATGATGCTG 
CAGCTGGGTG GCACAGTCAT TGGCAGTGCC CGCTGTCAGG ACTTCCGCAC TAAAGAGGGT 
CGAGCTAAAG CCGCTTGTAA CCTGGTGAAG CTGGGCATCA CTAATCTGTG TGTGATCGGT 
GGAGATGGCA GTCTGACCGG TGCCAATGAG TTCCGCAATG AGTGGAGTGA ACTGCTCCAG 
ATCTTGCAGA AAACAGGAAA GATCACAGCA GAGGAGGCCA AATGTTCATC TCATCTAAAC 
ATCGTCGGGA TGGTTGGATC CATAGACAAC GATTTCTGTG GCACGGACAT GACAATCGGC 
ACAGATTCAG CTCTGCACAG GATCATGGAG GTAGTGGACG CCATCACTAC AACAGCTCAG 
AGTCATCAAA GAGCCTTCAT TCTGGAAGTG ATGGGGAGGC ATTGTGGGTA TTTGGCCTTA 
GTCACTGCCC TTTCATGTGG TGCTGACTGG GTATTTATCC CCGAAATGCC CCCAGATGAA 
GGATGGGAGG ACCATCTGTG CAGGAGACTC ACTTACCAAA GAAGCATTGG CAATCGTTTG 
AATGTCATCA TTGTGGCCGA AGGAGCTTTG GATCGACACG GCAAACCCAT CACCTGTGAC 
ATTATCAAGA ACCTGGTGAC TAAGAAGCTT GGTTTTGACA CTCGGGCCAC TATTTTGGGC 
CACGTGCAGA GAGGAGGAAC ACCTTCAGCC TTCGACAGAA TATTGGGCAG CAGGATGGGT 
GTGGAGGCTG TGATGGCGCT CCTGGAAGCC ACTCCTGACA CCCCTGCCTG TGTGGTCAGT 
CTGTCTGGAA ACATGGCTGT TCGTCTGCCC CTCATGGAGT GTGTGCAAGT GACAAAAGAG 
GTGACCAAAG CCATGGCCGA GGGCAGATTT GAGGAAGCTA TTAAGCTCAG GGGAAAGAGT 
TTTGAGAACA ATTGGAACAC ATATAAACTC TTAGCCCATG TGACGGCCCC AGATGTGAAG 
AGTAATATAA ACATAGCTAT TCTGAATGTG GGAGCTCCCT GTGCAGGAAT GAACGCAGCA 
GTCCGTTCAG CAGTCAGGAT CGGCATCCTT CAAGGACACA ACATGCTGGC TGTTCATGAC 
GGCTTCGAGG GACTCGCAAA TGGAACTATT GAACCAATGG CATGGGGTTA TGTGGGAGGC 
TGGACGGGCA AAGGTGGATC TATTTTAGGA ACCAAGAGGT CACTGCCGGC TAATATGATT 
GAAGAAATCA GCCTGAATAT AGCCAAGTTT AACATCCATG CCCTGGTCAT CATTGGAGGA 
TTTGAAGCCT TTGTTGGAGG CTTGGAATTA GTGACTGCCA GAGAAAAATA CGAGGAGTTG 
TGTATTCCTC TGGTTGTTAT TCCAGCAACT GTGTCTAATA ATGTTCCTGG ATCAGACTTC 
AGCGTTGGTG CTGATACTGC GCTTAATACT ATCACAACTA CATGCGATAG GATCAAGCAA 
TCTGCTGCTG GAACGAAGCG CAGGGTTTTC ATCATTGAAA CAATGGGAGG ATACTGTGGT 
TATCTGGCAA CCATGGCAGG ACTGGCTGCA GGGGCTGACG CTGCGTACGT CTTTGAAGAC 
CCCTTTGGCA TTCATGACCT GGAGGCCAAC GTGGAGCACT TACTGGAGAA GATGAAGACA 
ACAGTGAAAA GAGGCCTCAT CCTCAGAAAT GAGAAATGTA ATGCAAACTA CACTACAGAT 
TTTCTCTTCA ACCTCTACTC AGAAGAGGGC AAAGGAGTGT TTGACTGCCG CAAAAATGTT 
CTTGGCCACA TGCAGCAGGG TGGAACCCCT ACACCGTTTG ATAGAAATTT TGGCACAAAG 
ATGGGAGCTA AAGCTGTGCT GTGGCTGACA GATAAACTGA AGGAGTGTTA CAGACATGGC 
CGTATCTTTG CCAACACGCC GGACTCTGCC TGTGTCCTGG GAATGAGGAA GAGAGCCATG 
CTCTTCCAGC CTCTCTCTGA TCTCAAGGAG GACACTGACT TTGAACATCG TATACCGAAG 
ACTGAGTGGT GGCTGAAGCT TAGACCCATT CTCAAAATTC TGGCCAAATA CAAGATCAGT 
TTGGACACCT CAGAGACTGC TACGATGGAG CATGTTATCA AGAAGAGAGG AGGAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300828.1
CDS	223..2922
Translation	METVWDNCVCVCQLCVCVWDVFVCVCQISVCLLELCVCLLRLSVLLFHWIPHIGLTVWIWQRTHRKTQFDANHVYGSKDPSYCDESKTEVLADGKTTSTTCFDSGLDDLSCSSATHNSCRLFEMQHSAPVDPTKMGVGRAIAVLTSGGDAQGMNAAVRATVRVGIYTGAKVYFVHEGYQGLVDGGDNIRQATWESVSMMLQLGGTVIGSARCQDFRTKEGRAKAACNLVKLGITNLCVIGGDGSLTGANEFRNEWSELLQILQKTGKITAEEAKCSSHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIMEVVDAITTTAQSHQRAFILEVMGRHCGYLALVTALSCGADWVFIPEMPPDEGWEDHLCRRLTYQRSIGNRLNVIIVAEGALDRHGKPITCDIIKNLVTKKLGFDTRATILGHVQRGGTPSAFDRILGSRMGVEAVMALLEATPDTPACVVSLSGNMAVRLPLMECVQVTKEVTKAMAEGRFEEAIKLRGKSFENNWNTYKLLAHVTAPDVKSNINIAILNVGAPCAGMNAAVRSAVRIGILQGHNMLAVHDGFEGLANGTIEPMAWGYVGGWTGKGGSILGTKRSLPANMIEEISLNIAKFNIHALVIIGGFEAFVGGLELVTAREKYEELCIPLVVIPATVSNNVPGSDFSVGADTALNTITTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYVFEDPFGIHDLEANVEHLLEKMKTTVKRGLILRNEKCNANYTTDFLFNLYSEEGKGVFDCRKNVLGHMQQGGTPTPFDRNFGTKMGAKAVLWLTDKLKECYRHGRIFANTPDSACVLGMRKRAMLFQPLSDLKEDTDFEHRIPKTEWWLKLRPILKILAKYKISLDTSETATMEHVIKKRGGV

Target ORF information:

RefSeq Version	XM_009302553.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302553.2

ORF Insert Sequence:

ATGGAGACTG TGTGGGACAA TTGCGTGTGT GTCTGCCAGC TGTGTGTATG CGTTTGGGAT 
GTGTTTGTAT GCGTGTGCCA GATTAGTGTT TGTTTGTTAG AGCTGTGTGT GTGTTTACTA 
CGACTTTCTG TACTGCTGTT CCACTGGATC CCTCATATCG GACTGACGGT GTGGATCTGG 
CAACGCACAC ATAGGAAGAC TCAGTTTGAT GCTAATCATG TTTACGGATC AAAAGATCCA 
AGTTACTGTG ATGAGTCAAA GACTGAAGTA CTTGCTGATG GAAAGACTAC TTCTACAACG 
TGTTTCGATA GTGGCCTGGA TGATCTCAGC TGTTCCTCCG CCACGCACAA TAGCTGCCGT 
CTCTTCGAAA TGCAGCATTC GGCACCTGTG GACCCCACAA AGATGGGGGT TGGAAGGGCT 
ATTGCTGTCC TCACCTCTGG AGGAGATGCA CAGGGTATGA ATGCTGCTGT TCGAGCCACG 
GTGAGAGTTG GGATCTACAC TGGTGCAAAG GTTTATTTCG TGCATGAGGG CTATCAGGGC 
CTGGTTGATG GCGGAGATAA TATCCGACAA GCCACTTGGG AGAGCGTGTC AATGATGCTG 
CAGCTGGGTG GCACAGTCAT TGGCAGTGCC CGCTGTCAGG ACTTCCGCAC TAAAGAGGGT 
CGAGCTAAAG CCGCTTGTAA CCTGGTGAAG CTGGGCATCA CTAATCTGTG TGTGATCGGT 
GGAGATGGCA GTCTGACCGG TGCCAATGAG TTCCGCAATG AGTGGAGTGA ACTGCTCCAG 
ATCTTGCAGA AAACAGGAAA GATCACAGCA GAGGAGGCCA AATGTTCATC TCATCTAAAC 
ATCGTCGGGA TGGTTGGATC CATAGACAAC GATTTCTGTG GCACGGACAT GACAATCGGC 
ACAGATTCAG CTCTGCACAG GATCATGGAG GTAGTGGACG CCATCACTAC AACAGCTCAG 
AGTCATCAAA GAGCCTTCAT TCTGGAAGTG ATGGGGAGGC ATTGTGGGTA TTTGGCCTTA 
GTCACTGCCC TTTCATGTGG TGCTGACTGG GTATTTATCC CCGAAATGCC CCCAGATGAA 
GGATGGGAGG ACCATCTGTG CAGGAGACTC ACTTACCAAA GAAGCATTGG CAATCGTTTG 
AATGTCATCA TTGTGGCCGA AGGAGCTTTG GATCGACACG GCAAACCCAT CACCTGTGAC 
ATTATCAAGA ACCTGGTGAC TAAGAAGCTT GGTTTTGACA CTCGGGCCAC TATTTTGGGC 
CACGTGCAGA GAGGAGGAAC ACCTTCAGCC TTCGACAGAA TATTGGGCAG CAGGATGGGT 
GTGGAGGCTG TGATGGCGCT CCTGGAAGCC ACTCCTGACA CCCCTGCCTG TGTGGTCAGT 
CTGTCTGGAA ACATGGCTGT TCGTCTGCCC CTCATGGAGT GTGTGCAAGT GACAAAAGAG 
GTGACCAAAG CCATGGCCGA GGGCAGATTT GAGGAAGCTA TTAAGCTCAG GGGAAAGAGT 
TTTGAGAACA ATTGGAACAC ATATAAACTC TTAGCCCATG TGACGGCCCC AGATGTGAAG 
AGTAATATAA ACATAGCTAT TCTGAATGTG GGAGCTCCCT GTGCAGGAAT GAACGCAGCA 
GTCCGTTCAG CAGTCAGGAT CGGCATCCTT CAAGGACACA ACATGCTGGC TGTTCATGAC 
GGCTTCGAGG GACTCGCAAA TGGAACTATT GAACCAATGG CATGGGGTTA TGTGGGAGGC 
TGGACGGGCA AAGGTGGATC TATTTTAGGA ACCAAGAGGT CACTGCCGGC TAATATGATT 
GAAGAAATCA GCCTGAATAT AGCCAAGTTT AACATCCATG CCCTGGTCAT CATTGGAGGA 
TTTGAAGCCT TTGTTGGAGG CTTGGAATTA GTGACTGCCA GAGAAAAATA CGAGGAGTTG 
TGTATTCCTC TGGTTGTTAT TCCAGCAACT GTGTCTAATA ATGTTCCTGG ATCAGACTTC 
AGCGTTGGTG CTGATACTGC GCTTAATACT ATCACAACTA CATGCGATAG GATCAAGCAA 
TCTGCTGCTG GAACGAAGCG CAGGGTTTTC ATCATTGAAA CAATGGGAGG ATACTGTGGT 
TATCTGGCAA CCATGGCAGG ACTGGCTGCA GGGGCTGACG CTGCGTACGT CTTTGAAGAC 
CCCTTTGGCA TTCATGACCT GGAGGCCAAC GTGGAGCACT TACTGGAGAA GATGAAGACA 
ACAGTGAAAA GAGGCCTCAT CCTCAGAAAT GAGAAATGTA ATGCAAACTA CACTACAGAT 
TTTCTCTTCA ACCTCTACTC AGAAGAGGGC AAAGGAGTGT TTGACTGCCG CAAAAATGTT 
CTTGGCCACA TGCAGCAGGG TGGAACCCCT ACACCGTTTG ATAGAAATTT TGGCACAAAG 
ATGGGAGCTA AAGCTGTGCT GTGGCTGACA GATAAACTGA AGGAGTGTTA CAGACATGGC 
CGTATCTTTG CCAACACGCC GGACTCTGCC TGTGTCCTGG GAATGAGGAA GAGAGCCATG 
CTCTTCCAGC CTCTCTCTGA TCTCAAGGAG GACACTGACT TTGAACATCG TATACCGAAG 
ACTGAGTGGT GGCTGAAGCT TAGACCCATT CTCAAAATTC TGGCCAAATA CAAGATCAGT 
TTGGACACCT CAGAGACTGC TACGATGGAG CATGTTATCA AGAAGAGAGG AGGAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfkmb ( NM_001089417.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001082886.2 Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa159716
Clone ID Related Accession (Same CDS sequence)	NM_001089417.2
Accession Version	NM_001089417.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-20
Organism	Danio rerio(zebrafish)
Product	phosphofructokinase, muscle b isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL954698.9. On Aug 17, 2018 this sequence version replaced NM_001089417.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC139532.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCAGCATT CGGCACCTGT GGACCCCACA AAGATGGGGG TTGGAAGGGC TATTGCTGTC 
CTCACCTCTG GAGGAGATGC ACAGGGTATG AATGCTGCTG TTCGAGCCAC GGTGAGAGTT 
GGGATCTACA CTGGTGCAAA GGTTTATTTC GTGCATGAGG GCTATCAGGG CCTGGTTGAT 
GGCGGAGATA ATATCCGACA AGCCACTTGG GAGAGCGTGT CAATGATGCT GCAGCTGGGT 
GGCACAGTCA TTGGCAGTGC CCGCTGTCAG GACTTCCGCA CTAAAGAGGG TCGAGCTAAA 
GCCGCTTGTA ACCTGGTGAA GCTGGGCATC ACTAATCTGT GTGTGATCGG TGGAGATGGC 
AGTCTGACCG GTGCCAATGA GTTCCGCAAT GAGTGGAGTG AACTGCTCCA GATCTTGCAG 
AAAACAGGAA AGATCACAGC AGAGGAGGCC AAATGTTCAT CTCATCTAAA CATCGTCGGG 
ATGGTTGGAT CCATAGACAA CGATTTCTGT GGCACGGACA TGACAATCGG CACAGATTCA 
GCTCTGCACA GGATCATGGA GGTAGTGGAC GCCATCACTA CAACAGCTCA GAGTCATCAA 
AGAGCCTTCA TTCTGGAAGT GATGGGGAGG CATTGTGGGT ATTTGGCCTT AGTCACTGCC 
CTTTCATGTG GTGCTGACTG GGTATTTATC CCCGAAATGC CCCCAGATGA AGGATGGGAG 
GACCATCTGT GCAGGAGACT CACTTACCAA AGAAGCATTG GCAATCGTTT GAATGTCATC 
ATTGTGGCCG AAGGAGCTTT GGATCGACAC GGCAAACCCA TCACCTGTGA CATTATCAAG 
AACCTGGTGA CTAAGAAGCT TGGTTTTGAC ACTCGGGCCA CTATTTTGGG CCACGTGCAG 
AGAGGAGGAA CACCTTCAGC CTTCGACAGA ATATTGGGCA GCAGGATGGG TGTGGAGGCT 
GTGATGGCGC TCCTGGAAGC CACTCCTGAC ACCCCTGCCT GTGTGGTCAG TCTGTCTGGA 
AACATGGCTG TTCGTCTGCC CCTCATGGAG TGTGTGCAAG TGACAAAAGA GGTGACCAAA 
GCCATGGCCG AGGGCAGATT TGAGGAAGCT ATTAAGCTCA GGGGAAAGAG TTTTGAGAAC 
AATTGGAACA CATATAAACT CTTAGCCCAT GTGACGGCCC CAGATGTGAA GAGTAATATA 
AACATAGCTA TTCTGAATGT GGGAGCTCCC TGTGCAGGAA TGAACGCAGC AGTCCGTTCA 
GCAGTCAGGA TCGGCATCCT TCAAGGACAC AACATGCTGG CTGTTCATGA CGGCTTCGAG 
GGACTCGCAA ATGGAACTAT TGAACCAATG GCATGGGGTT ATGTGGGAGG CTGGACGGGC 
AAAGGTGGAT CTATTTTAGG AACCAAGAGG TCACTGCCGG CTAATATGAT TGAAGAAATC 
AGCCTGAATA TAGCCAAGTT TAACATCCAT GCCCTGGTCA TCATTGGAGG ATTTGAAGCC 
TTTGTTGGAG GCTTGGAATT AGTGACTGCC AGAGAAAAAT ACGAGGAGTT GTGTATTCCT 
CTGGTTGTTA TTCCAGCAAC TGTGTCTAAT AATGTTCCTG GATCAGACTT CAGCGTTGGT 
GCTGATACTG CGCTTAATAC TATCACAACT ACATGCGATA GGATCAAGCA ATCTGCTGCT 
GGAACGAAGC GCAGGGTTTT CATCATTGAA ACAATGGGAG GATACTGTGG TTATCTGGCA 
ACCATGGCAG GACTGGCTGC AGGGGCTGAC GCTGCGTACG TCTTTGAAGA CCCCTTTGGC 
ATTCATGACC TGGAGGCCAA CGTGGAGCAC TTACTGGAGA AGATGAAGAC AACAGTGAAA 
AGAGGCCTCA TCCTCAGAAA TGAGAAATGT AATGCAAACT ACACTACAGA TTTTCTCTTC 
AACCTCTACT CAGAAGAGGG CAAAGGAGTG TTTGACTGCC GCAAAAATGT TCTTGGCCAC 
ATGCAGCAGG GTGGAACCCC TACACCGTTT GATAGAAATT TTGGCACAAA GATGGGAGCT 
AAAGCTGTGC TGTGGCTGAC AGATAAACTG AAGGAGTGTT ACAGACATGG CCGTATCTTT 
GCCAACACGC CGGACTCTGC CTGTGTCCTG GGAATGAGGA AGAGAGCCAT GCTCTTCCAG 
CCTCTCTCTG ATCTCAAGGA GGACACTGAC TTTGAACATC GTATACCGAA GACTGAGTGG 
TGGCTGAAGC TTAGACCCAT TCTCAAAATT CTGGCCAAAT ACAAGATCAG TTTGGACACC 
TCAGAGACTG CTACGATGGA GCATGTTATC AAGAAGAGAG GAGGAGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082886.2
CDS	78..2408
Translation	MQHSAPVDPTKMGVGRAIAVLTSGGDAQGMNAAVRATVRVGIYTGAKVYFVHEGYQGLVDGGDNIRQATWESVSMMLQLGGTVIGSARCQDFRTKEGRAKAACNLVKLGITNLCVIGGDGSLTGANEFRNEWSELLQILQKTGKITAEEAKCSSHLNIVGMVGSIDNDFCGTDMTIGTDSALHRIMEVVDAITTTAQSHQRAFILEVMGRHCGYLALVTALSCGADWVFIPEMPPDEGWEDHLCRRLTYQRSIGNRLNVIIVAEGALDRHGKPITCDIIKNLVTKKLGFDTRATILGHVQRGGTPSAFDRILGSRMGVEAVMALLEATPDTPACVVSLSGNMAVRLPLMECVQVTKEVTKAMAEGRFEEAIKLRGKSFENNWNTYKLLAHVTAPDVKSNINIAILNVGAPCAGMNAAVRSAVRIGILQGHNMLAVHDGFEGLANGTIEPMAWGYVGGWTGKGGSILGTKRSLPANMIEEISLNIAKFNIHALVIIGGFEAFVGGLELVTAREKYEELCIPLVVIPATVSNNVPGSDFSVGADTALNTITTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYVFEDPFGIHDLEANVEHLLEKMKTTVKRGLILRNEKCNANYTTDFLFNLYSEEGKGVFDCRKNVLGHMQQGGTPTPFDRNFGTKMGAKAVLWLTDKLKECYRHGRIFANTPDSACVLGMRKRAMLFQPLSDLKEDTDFEHRIPKTEWWLKLRPILKILAKYKISLDTSETATMEHVIKKRGGV

Target ORF information:

RefSeq Version	NM_001089417.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphofructokinase, muscle b (pfkmb), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089417.2

ORF Insert Sequence:

ATGCAGCATT CGGCACCTGT GGACCCCACA AAGATGGGGG TTGGAAGGGC TATTGCTGTC 
CTCACCTCTG GAGGAGATGC ACAGGGTATG AATGCTGCTG TTCGAGCCAC GGTGAGAGTT 
GGGATCTACA CTGGTGCAAA GGTTTATTTC GTGCATGAGG GCTATCAGGG CCTGGTTGAT 
GGCGGAGATA ATATCCGACA AGCCACTTGG GAGAGCGTGT CAATGATGCT GCAGCTGGGT 
GGCACAGTCA TTGGCAGTGC CCGCTGTCAG GACTTCCGCA CTAAAGAGGG TCGAGCTAAA 
GCCGCTTGTA ACCTGGTGAA GCTGGGCATC ACTAATCTGT GTGTGATCGG TGGAGATGGC 
AGTCTGACCG GTGCCAATGA GTTCCGCAAT GAGTGGAGTG AACTGCTCCA GATCTTGCAG 
AAAACAGGAA AGATCACAGC AGAGGAGGCC AAATGTTCAT CTCATCTAAA CATCGTCGGG 
ATGGTTGGAT CCATAGACAA CGATTTCTGT GGCACGGACA TGACAATCGG CACAGATTCA 
GCTCTGCACA GGATCATGGA GGTAGTGGAC GCCATCACTA CAACAGCTCA GAGTCATCAA 
AGAGCCTTCA TTCTGGAAGT GATGGGGAGG CATTGTGGGT ATTTGGCCTT AGTCACTGCC 
CTTTCATGTG GTGCTGACTG GGTATTTATC CCCGAAATGC CCCCAGATGA AGGATGGGAG 
GACCATCTGT GCAGGAGACT CACTTACCAA AGAAGCATTG GCAATCGTTT GAATGTCATC 
ATTGTGGCCG AAGGAGCTTT GGATCGACAC GGCAAACCCA TCACCTGTGA CATTATCAAG 
AACCTGGTGA CTAAGAAGCT TGGTTTTGAC ACTCGGGCCA CTATTTTGGG CCACGTGCAG 
AGAGGAGGAA CACCTTCAGC CTTCGACAGA ATATTGGGCA GCAGGATGGG TGTGGAGGCT 
GTGATGGCGC TCCTGGAAGC CACTCCTGAC ACCCCTGCCT GTGTGGTCAG TCTGTCTGGA 
AACATGGCTG TTCGTCTGCC CCTCATGGAG TGTGTGCAAG TGACAAAAGA GGTGACCAAA 
GCCATGGCCG AGGGCAGATT TGAGGAAGCT ATTAAGCTCA GGGGAAAGAG TTTTGAGAAC 
AATTGGAACA CATATAAACT CTTAGCCCAT GTGACGGCCC CAGATGTGAA GAGTAATATA 
AACATAGCTA TTCTGAATGT GGGAGCTCCC TGTGCAGGAA TGAACGCAGC AGTCCGTTCA 
GCAGTCAGGA TCGGCATCCT TCAAGGACAC AACATGCTGG CTGTTCATGA CGGCTTCGAG 
GGACTCGCAA ATGGAACTAT TGAACCAATG GCATGGGGTT ATGTGGGAGG CTGGACGGGC 
AAAGGTGGAT CTATTTTAGG AACCAAGAGG TCACTGCCGG CTAATATGAT TGAAGAAATC 
AGCCTGAATA TAGCCAAGTT TAACATCCAT GCCCTGGTCA TCATTGGAGG ATTTGAAGCC 
TTTGTTGGAG GCTTGGAATT AGTGACTGCC AGAGAAAAAT ACGAGGAGTT GTGTATTCCT 
CTGGTTGTTA TTCCAGCAAC TGTGTCTAAT AATGTTCCTG GATCAGACTT CAGCGTTGGT 
GCTGATACTG CGCTTAATAC TATCACAACT ACATGCGATA GGATCAAGCA ATCTGCTGCT 
GGAACGAAGC GCAGGGTTTT CATCATTGAA ACAATGGGAG GATACTGTGG TTATCTGGCA 
ACCATGGCAG GACTGGCTGC AGGGGCTGAC GCTGCGTACG TCTTTGAAGA CCCCTTTGGC 
ATTCATGACC TGGAGGCCAA CGTGGAGCAC TTACTGGAGA AGATGAAGAC AACAGTGAAA 
AGAGGCCTCA TCCTCAGAAA TGAGAAATGT AATGCAAACT ACACTACAGA TTTTCTCTTC 
AACCTCTACT CAGAAGAGGG CAAAGGAGTG TTTGACTGCC GCAAAAATGT TCTTGGCCAC 
ATGCAGCAGG GTGGAACCCC TACACCGTTT GATAGAAATT TTGGCACAAA GATGGGAGCT 
AAAGCTGTGC TGTGGCTGAC AGATAAACTG AAGGAGTGTT ACAGACATGG CCGTATCTTT 
GCCAACACGC CGGACTCTGC CTGTGTCCTG GGAATGAGGA AGAGAGCCAT GCTCTTCCAG 
CCTCTCTCTG ATCTCAAGGA GGACACTGAC TTTGAACATC GTATACCGAA GACTGAGTGG 
TGGCTGAAGC TTAGACCCAT TCTCAAAATT CTGGCCAAAT ACAAGATCAG TTTGGACACC 
TCAGAGACTG CTACGATGGA GCATGTTATC AAGAAGAGAG GAGGAGTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Zebrafish embryos as a screen for DNA methylation modifications after compound exposure.
Toxicology and applied pharmacology29184-96(2016 Jan)
Bouwmeester MC,Ruiter S,Lommelaars T,Sippel J,Hodemaekers HM,van den Brandhof EJ,Pennings JL,Kamstra JH,Jelinek J,Issa JP,Legler J,van der Ven LT

Global protein expression profiling of zebrafish organs based on in vivo incorporation of stable isotopes.
Journal of proteome research13(4)2162-74(2014 Apr)
Nolte H,Konzer A,Ruhs A,Jungblut B,Braun T,Kr?ger M

GLUT10 is required for the development of the cardiovascular system and the notochord and connects mitochondrial function to TGF? signaling.
Human molecular genetics21(6)1248-59(2012 Mar)
Willaert A,Khatri S,Callewaert BL,Coucke PJ,Crosby SD,Lee JG,Davis EC,Shiva S,Tsang M,De Paepe A,Urban Z

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

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pfkmb cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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